LOCUS       DACJGB010000001       113717 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.001, whole genome shotgun sequence.
ACCESSION   DACJGB010000001 DACJGB010000000
VERSION     DACJGB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 113717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 113717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..113717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.001"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(218..1015)
                     /locus_tag="IYI34_000001"
     CDS             complement(218..1015)
                     /locus_tag="IYI34_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAR0557908.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IYI34_000002"
     CDS             complement(1012..2196)
                     /locus_tag="IYI34_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAR0557909.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IYI34_000003"
     CDS             complement(2221..3468)
                     /locus_tag="IYI34_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAR0557910.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IYI34_000004"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IYI34_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAR0557911.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IYI34_000005"
     CDS             5448..5762
                     /locus_tag="IYI34_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAR0557912.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IYI34_000006"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IYI34_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAR0557913.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IYI34_000007"
     CDS             6447..7091
                     /locus_tag="IYI34_000007"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAR0557914.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IYI34_000008"
     CDS             7106..7435
                     /locus_tag="IYI34_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0557915.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IYI34_000009"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IYI34_000009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAR0557916.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IYI34_000010"
     CDS             8423..9247
                     /locus_tag="IYI34_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAR0557917.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IYI34_000011"
     CDS             9240..9587
                     /locus_tag="IYI34_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAR0557918.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IYI34_000012"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IYI34_000012"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAR0557919.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IYI34_000013"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IYI34_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAR0557920.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IYI34_000014"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IYI34_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAR0557921.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IYI34_000015"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IYI34_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAR0557922.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IYI34_000016"
     CDS             15244..16011
                     /locus_tag="IYI34_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0557923.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IYI34_000017"
     CDS             16001..18043
                     /locus_tag="IYI34_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0557924.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IYI34_000018"
     CDS             18324..18473
                     /locus_tag="IYI34_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAR0557925.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IYI34_000019"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IYI34_000019"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAR0557926.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IYI34_000020"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IYI34_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAR0557927.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IYI34_000021"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IYI34_000021"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAR0557928.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IYI34_000022"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IYI34_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAR0557929.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IYI34_000023"
     CDS             22890..23972
                     /locus_tag="IYI34_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAR0557930.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IYI34_000024"
     CDS             23972..24775
                     /locus_tag="IYI34_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAR0557931.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IYI34_000025"
     CDS             24845..25585
                     /locus_tag="IYI34_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR0557932.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IYI34_000026"
     CDS             25596..26600
                     /locus_tag="IYI34_000026"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAR0557933.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IYI34_000027"
     CDS             complement(26645..28489)
                     /locus_tag="IYI34_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0557934.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28700..29218
                     /locus_tag="IYI34_000028"
     CDS             28700..29218
                     /locus_tag="IYI34_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAR0557935.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29346..30029
                     /locus_tag="IYI34_000029"
     CDS             29346..30029
                     /locus_tag="IYI34_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAR0557936.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30338..30411
                     /locus_tag="IYI34_000030"
     tRNA            30338..30411
                     /locus_tag="IYI34_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30372..30374,aa:Arg,seq:tct)
     gene            30570..30929
                     /locus_tag="IYI34_000031"
     CDS             30570..30929
                     /locus_tag="IYI34_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAR0557937.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31052..32683
                     /locus_tag="IYI34_000032"
     CDS             31052..32683
                     /locus_tag="IYI34_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAR0557938.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32745..33386)
                     /locus_tag="IYI34_000033"
     CDS             complement(32745..33386)
                     /locus_tag="IYI34_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAR0557939.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33668..33976
                     /gene="rplU"
                     /locus_tag="IYI34_000034"
     CDS             33668..33976
                     /gene="rplU"
                     /locus_tag="IYI34_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAR0557940.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34003..34347
                     /locus_tag="IYI34_000035"
     CDS             34003..34347
                     /locus_tag="IYI34_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAR0557941.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34360..34653
                     /gene="rpmA"
                     /locus_tag="IYI34_000036"
     CDS             34360..34653
                     /gene="rpmA"
                     /locus_tag="IYI34_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAR0557942.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35060..37894
                     /locus_tag="IYI34_000037"
     CDS             35060..37894
                     /locus_tag="IYI34_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0557943.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38085..38576
                     /locus_tag="IYI34_000038"
     CDS             38085..38576
                     /locus_tag="IYI34_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAR0557944.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38679..39668
                     /gene="manX"
                     /locus_tag="IYI34_000039"
     CDS             38679..39668
                     /gene="manX"
                     /locus_tag="IYI34_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAR0557945.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39701..40504
                     /locus_tag="IYI34_000040"
     CDS             39701..40504
                     /locus_tag="IYI34_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAR0557946.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40525..41439
                     /locus_tag="IYI34_000041"
     CDS             40525..41439
                     /locus_tag="IYI34_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAR0557947.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41522..41896
                     /locus_tag="IYI34_000042"
     CDS             41522..41896
                     /locus_tag="IYI34_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAR0557948.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42035..42280
                     /locus_tag="IYI34_000043"
     CDS             42035..42280
                     /locus_tag="IYI34_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR0557949.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42452..43516
                     /locus_tag="IYI34_000044"
     CDS             42452..43516
                     /locus_tag="IYI34_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAR0557950.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43762..44187
                     /locus_tag="IYI34_000045"
     CDS             43762..44187
                     /locus_tag="IYI34_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR0557951.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44180..44629
                     /gene="nusB"
                     /locus_tag="IYI34_000046"
     CDS             44180..44629
                     /gene="nusB"
                     /locus_tag="IYI34_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAR0557952.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44726..45580
                     /locus_tag="IYI34_000047"
     CDS             44726..45580
                     /locus_tag="IYI34_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAR0557953.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45581..46918
                     /locus_tag="IYI34_000048"
     CDS             45581..46918
                     /locus_tag="IYI34_000048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAR0557954.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46923..47147
                     /locus_tag="IYI34_000049"
     CDS             46923..47147
                     /locus_tag="IYI34_000049"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAR0557955.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47149..48039
                     /locus_tag="IYI34_000050"
     CDS             47149..48039
                     /locus_tag="IYI34_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAR0557956.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48048..48866
                     /locus_tag="IYI34_000051"
     CDS             48048..48866
                     /locus_tag="IYI34_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAR0557957.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48973..49422
                     /locus_tag="IYI34_000052"
     CDS             48973..49422
                     /locus_tag="IYI34_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAR0557958.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49440..51122
                     /gene="recN"
                     /locus_tag="IYI34_000053"
     CDS             49440..51122
                     /gene="recN"
                     /locus_tag="IYI34_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAR0557959.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51177..51293)
                     /locus_tag="IYI34_000054"
     CDS             complement(51177..51293)
                     /locus_tag="IYI34_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAR0557960.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51366..52322)
                     /locus_tag="IYI34_000055"
     CDS             complement(51366..52322)
                     /locus_tag="IYI34_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAR0557961.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52493..52861
                     /locus_tag="IYI34_000056"
     CDS             52493..52861
                     /locus_tag="IYI34_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAR0557962.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53031..53462
                     /gene="mraZ"
                     /locus_tag="IYI34_000057"
     CDS             53031..53462
                     /gene="mraZ"
                     /locus_tag="IYI34_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAR0557963.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53483..54442
                     /gene="rsmH"
                     /locus_tag="IYI34_000058"
     CDS             53483..54442
                     /gene="rsmH"
                     /locus_tag="IYI34_000058"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAR0557964.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54447..54848
                     /gene="ftsL"
                     /locus_tag="IYI34_000059"
     CDS             54447..54848
                     /gene="ftsL"
                     /locus_tag="IYI34_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAR0557965.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54849..57041
                     /locus_tag="IYI34_000060"
     CDS             54849..57041
                     /locus_tag="IYI34_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAR0557966.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57067..58029
                     /locus_tag="IYI34_000061"
     CDS             57067..58029
                     /locus_tag="IYI34_000061"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAR0557967.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58043..59404
                     /locus_tag="IYI34_000062"
     CDS             58043..59404
                     /locus_tag="IYI34_000062"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAR0557968.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59430..60518
                     /gene="murG"
                     /locus_tag="IYI34_000063"
     CDS             59430..60518
                     /gene="murG"
                     /locus_tag="IYI34_000063"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAR0557969.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60533..61753
                     /locus_tag="IYI34_000064"
     CDS             60533..61753
                     /locus_tag="IYI34_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAR0557970.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61909..63234
                     /gene="ftsA"
                     /locus_tag="IYI34_000065"
     CDS             61909..63234
                     /gene="ftsA"
                     /locus_tag="IYI34_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAR0557971.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63257..64498
                     /gene="ftsZ"
                     /locus_tag="IYI34_000066"
     CDS             63257..64498
                     /gene="ftsZ"
                     /locus_tag="IYI34_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAR0557972.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64515..65192
                     /locus_tag="IYI34_000067"
     CDS             64515..65192
                     /locus_tag="IYI34_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAR0557973.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65206..65805
                     /locus_tag="IYI34_000068"
     CDS             65206..65805
                     /locus_tag="IYI34_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAR0557974.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65820..66107
                     /locus_tag="IYI34_000069"
     CDS             65820..66107
                     /locus_tag="IYI34_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAR0557975.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66156..66938
                     /locus_tag="IYI34_000070"
     CDS             66156..66938
                     /locus_tag="IYI34_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAR0557976.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            66959..67666
                     /locus_tag="IYI34_000071"
     CDS             66959..67666
                     /locus_tag="IYI34_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAR0557977.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            67928..70726
                     /gene="ileS"
                     /locus_tag="IYI34_000072"
     CDS             67928..70726
                     /gene="ileS"
                     /locus_tag="IYI34_000072"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAR0557978.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70775..72301)
                     /locus_tag="IYI34_000073"
     CDS             complement(70775..72301)
                     /locus_tag="IYI34_000073"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAR0557979.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72316..72963)
                     /locus_tag="IYI34_000074"
     CDS             complement(72316..72963)
                     /locus_tag="IYI34_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAR0557980.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73147..73476)
                     /locus_tag="IYI34_000075"
     CDS             complement(73147..73476)
                     /locus_tag="IYI34_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAR0557981.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73653..74381
                     /gene="sfsA"
                     /locus_tag="IYI34_000076"
     CDS             73653..74381
                     /gene="sfsA"
                     /locus_tag="IYI34_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAR0557982.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74397..75410
                     /locus_tag="IYI34_000077"
     CDS             74397..75410
                     /locus_tag="IYI34_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR0557983.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75410..76687
                     /locus_tag="IYI34_000078"
     CDS             75410..76687
                     /locus_tag="IYI34_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAR0557984.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76750..79452
                     /locus_tag="IYI34_000079"
     CDS             76750..79452
                     /locus_tag="IYI34_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0557985.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79604..79921
                     /locus_tag="IYI34_000080"
     CDS             79604..79921
                     /locus_tag="IYI34_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0557986.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            79951..80271
                     /locus_tag="IYI34_000081"
     CDS             79951..80271
                     /locus_tag="IYI34_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAR0557987.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80379..81839
                     /locus_tag="IYI34_000082"
     CDS             80379..81839
                     /locus_tag="IYI34_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0557988.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81907..82128
                     /locus_tag="IYI34_000083"
     CDS             81907..82128
                     /locus_tag="IYI34_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0557989.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82159..82341
                     /locus_tag="IYI34_000084"
     CDS             82159..82341
                     /locus_tag="IYI34_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAR0557990.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82341..82754
                     /locus_tag="IYI34_000085"
     CDS             82341..82754
                     /locus_tag="IYI34_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAR0557991.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82877..84058
                     /locus_tag="IYI34_000086"
     CDS             82877..84058
                     /locus_tag="IYI34_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0557992.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84447..84520
                     /locus_tag="IYI34_000087"
     tRNA            84447..84520
                     /locus_tag="IYI34_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84481..84483,aa:Arg,seq:cct)
     gene            complement(84589..85728)
                     /locus_tag="IYI34_000088"
     CDS             complement(84589..85728)
                     /locus_tag="IYI34_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0557993.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(86027..86662)
                     /locus_tag="IYI34_000089"
     CDS             complement(86027..86662)
                     /locus_tag="IYI34_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0557994.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(86775..87410)
                     /locus_tag="IYI34_000090"
     CDS             complement(86775..87410)
                     /locus_tag="IYI34_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAR0557995.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(87444..87905)
                     /locus_tag="IYI34_000091"
     CDS             complement(87444..87905)
                     /locus_tag="IYI34_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAR0557996.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(88035..88466)
                     /locus_tag="IYI34_000092"
     CDS             complement(88035..88466)
                     /locus_tag="IYI34_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAR0557997.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(88484..88804)
                     /locus_tag="IYI34_000093"
     CDS             complement(88484..88804)
                     /locus_tag="IYI34_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0557998.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            89103..89879
                     /locus_tag="IYI34_000094"
     CDS             89103..89879
                     /locus_tag="IYI34_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAR0557999.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            89894..90097
                     /locus_tag="IYI34_000095"
     CDS             89894..90097
                     /locus_tag="IYI34_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558000.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            90113..90451
                     /locus_tag="IYI34_000096"
     CDS             90113..90451
                     /locus_tag="IYI34_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAR0558001.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            90438..90617
                     /locus_tag="IYI34_000097"
     CDS             90438..90617
                     /locus_tag="IYI34_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558002.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(90660..91130)
                     /locus_tag="IYI34_000098"
     CDS             complement(90660..91130)
                     /locus_tag="IYI34_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558003.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            91217..91915
                     /locus_tag="IYI34_000099"
     CDS             91217..91915
                     /locus_tag="IYI34_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAR0558004.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            92093..92434
                     /locus_tag="IYI34_000100"
     CDS             92093..92434
                     /locus_tag="IYI34_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558005.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            92427..93098
                     /locus_tag="IYI34_000101"
     CDS             92427..93098
                     /locus_tag="IYI34_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAR0558006.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            93104..93796
                     /locus_tag="IYI34_000102"
     CDS             93104..93796
                     /locus_tag="IYI34_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558007.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            93793..94542
                     /locus_tag="IYI34_000103"
     CDS             93793..94542
                     /locus_tag="IYI34_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558008.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            94554..94823
                     /locus_tag="IYI34_000104"
     CDS             94554..94823
                     /locus_tag="IYI34_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558009.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            94986..95285
                     /locus_tag="IYI34_000105"
     CDS             94986..95285
                     /locus_tag="IYI34_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558010.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            95285..95587
                     /locus_tag="IYI34_000106"
     CDS             95285..95587
                     /locus_tag="IYI34_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558011.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            95627..95992
                     /locus_tag="IYI34_000107"
     CDS             95627..95992
                     /locus_tag="IYI34_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558012.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            96619..96801
                     /locus_tag="IYI34_000108"
     CDS             96619..96801
                     /locus_tag="IYI34_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558013.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            96814..97008
                     /locus_tag="IYI34_000109"
     CDS             96814..97008
                     /locus_tag="IYI34_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558014.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(97041..97253)
                     /locus_tag="IYI34_000110"
     CDS             complement(97041..97253)
                     /locus_tag="IYI34_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558015.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            97378..97581
                     /locus_tag="IYI34_000111"
     CDS             97378..97581
                     /locus_tag="IYI34_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558016.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            97578..97850
                     /locus_tag="IYI34_000112"
     CDS             97578..97850
                     /locus_tag="IYI34_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HAR0558017.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            97851..98036
                     /locus_tag="IYI34_000113"
     CDS             97851..98036
                     /locus_tag="IYI34_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558018.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            98023..98370
                     /locus_tag="IYI34_000114"
     CDS             98023..98370
                     /locus_tag="IYI34_000114"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558019.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            98330..98806
                     /locus_tag="IYI34_000115"
     CDS             98330..98806
                     /locus_tag="IYI34_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HAR0558020.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(98952..99680)
                     /locus_tag="IYI34_000116"
     CDS             complement(98952..99680)
                     /locus_tag="IYI34_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAR0558021.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            99728..100006
                     /locus_tag="IYI34_000117"
     CDS             99728..100006
                     /locus_tag="IYI34_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558022.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            100008..100394
                     /locus_tag="IYI34_000118"
     CDS             100008..100394
                     /locus_tag="IYI34_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558023.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            100391..100771
                     /locus_tag="IYI34_000119"
     CDS             100391..100771
                     /locus_tag="IYI34_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAR0558024.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            100883..101335
                     /locus_tag="IYI34_000120"
     CDS             100883..101335
                     /locus_tag="IYI34_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HAR0558025.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            101332..103059
                     /locus_tag="IYI34_000121"
     CDS             101332..103059
                     /locus_tag="IYI34_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAR0558026.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            103123..104310
                     /locus_tag="IYI34_000122"
     CDS             103123..104310
                     /locus_tag="IYI34_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAR0558027.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            104276..104851
                     /locus_tag="IYI34_000123"
     CDS             104276..104851
                     /locus_tag="IYI34_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAR0558028.1"
                     /translation="MEMKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWD
                     DLSEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDT
                     TVARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYED
                     TEAALRSRDELEKNVEERKKLIKKINQALEA"
     gene            104864..106228
                     /locus_tag="IYI34_000124"
     CDS             104864..106228
                     /locus_tag="IYI34_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAR0558029.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            106230..106508
                     /locus_tag="IYI34_000125"
     CDS             106230..106508
                     /locus_tag="IYI34_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAR0558030.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            106489..106815
                     /locus_tag="IYI34_000126"
     CDS             106489..106815
                     /locus_tag="IYI34_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAR0558031.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            106805..107143
                     /locus_tag="IYI34_000127"
     CDS             106805..107143
                     /locus_tag="IYI34_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558032.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            107133..107498
                     /locus_tag="IYI34_000128"
     CDS             107133..107498
                     /locus_tag="IYI34_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAR0558033.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            107505..108131
                     /locus_tag="IYI34_000129"
     CDS             107505..108131
                     /locus_tag="IYI34_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR0558034.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            108131..108595
                     /locus_tag="IYI34_000130"
     CDS             108131..108595
                     /locus_tag="IYI34_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558035.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            108790..111099
                     /locus_tag="IYI34_000131"
     CDS             108790..111099
                     /locus_tag="IYI34_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAR0558036.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            111111..111815
                     /locus_tag="IYI34_000132"
     CDS             111111..111815
                     /locus_tag="IYI34_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR0558037.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            111812..>113717
                     /locus_tag="IYI34_000133"
     CDS             111812..>113717
                     /locus_tag="IYI34_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558038.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWRIYDILCNNGFTKQ
                     SACGILGNIQQ"
BASE COUNT        37720 a        19052 c        23760 g        33185 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaatttat cgttattata tgattaagaa ggattgaata
    28681 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
    28741 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
    28801 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
    28861 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
    28921 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
    28981 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
    29041 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
    29101 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
    29161 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
    29221 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
    29281 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
    29341 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
    29401 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
    29461 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
    29521 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
    29581 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
    29641 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
    29701 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
    29761 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
    29821 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
    29881 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
    29941 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
    30001 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
    30061 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
    30121 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
    30181 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
    30241 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
    30301 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
    30361 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
    30421 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
    30481 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
    30541 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
    30601 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
    30661 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
    30721 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
    30781 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
    30841 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
    30901 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
    30961 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
    31021 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
    31081 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
    31141 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
    31201 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
    31261 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
    31321 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
    31381 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
    31441 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
    31501 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
    31561 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
    31621 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
    31681 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
    31741 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
    31801 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
    31861 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
    31921 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
    31981 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
    32041 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
    32101 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
    32161 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
    32221 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
    32281 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
    32341 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
    32401 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
    32461 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
    32521 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
    32581 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
    32641 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
    32701 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
    32761 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
    32821 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
    32881 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
    32941 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
    33001 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
    33061 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
    33121 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
    33181 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
    33241 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
    33301 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
    33361 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
    33421 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
    33481 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
    33541 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
    33601 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
    33661 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
    33721 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
    33781 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
    33841 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
    33901 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
    33961 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
    34021 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
    34081 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
    34141 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
    34201 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
    34261 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
    34321 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
    34381 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
    34441 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
    34501 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
    34561 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
    34621 gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg
    34681 acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg
    34741 aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt
    34801 gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata
    34861 tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc
    34921 ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata
    34981 gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta
    35041 tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga
    35101 aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc
    35161 tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga
    35221 aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac
    35281 gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga
    35341 aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg
    35401 cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt
    35461 aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta
    35521 tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg
    35581 tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg
    35641 tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat
    35701 gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt
    35761 tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt
    35821 acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg
    35881 gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt
    35941 cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt
    36001 gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt
    36061 gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga
    36121 aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc
    36181 aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc
    36241 accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata
    36301 tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat
    36361 caatcatttc atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg
    36421 tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt
    36481 gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt
    36541 tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga
    36601 acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt
    36661 tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga
    36721 agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat
    36781 cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca
    36841 acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa
    36901 aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat
    36961 actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg
    37021 aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa
    37081 ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg
    37141 ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa
    37201 agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat
    37261 cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt
    37321 ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg
    37381 gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa
    37441 tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat
    37501 ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat
    37561 ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca
    37621 gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc
    37681 aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac
    37741 aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga
    37801 tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa
    37861 tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata
    37921 gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa
    37981 agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat
    38041 gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg
    38101 cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag
    38161 gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc
    38221 tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga
    38281 ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc
    38341 cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg
    38401 gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag
    38461 atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac
    38521 ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag
    38581 aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt
    38641 tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa
    38701 gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag
    38761 aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat
    38821 tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat
    38881 ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg
    38941 caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa
    39001 tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag
    39061 taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac
    39121 ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag
    39181 cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc
    39241 aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac
    39301 taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga
    39361 tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc
    39421 ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt
    39481 caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag
    39541 acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac
    39601 ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa
    39661 aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat
    39721 tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt
    39781 tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg
    39841 tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc
    39901 tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg
    39961 acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg
    40021 gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc
    40081 ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt
    40141 acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt
    40201 gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg
    40261 aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg
    40321 gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct
    40381 aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc
    40441 atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta
    40501 ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat
    40561 cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg
    40621 caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa
    40681 gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt
    40741 gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct
    40801 gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt
    40861 ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta
    40921 gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt
    40981 tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat
    41041 gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc
    41101 gtattagctg cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact
    41161 gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga
    41221 atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca
    41281 acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg
    41341 ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc
    41401 gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg
    41461 cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa
    41521 aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg
    41581 tctgacagat tacgggcaaa tcatgatagg taataatggt tttgagtttt accatgcgcg
    41641 agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt
    41701 catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata
    41761 cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga
    41821 agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat
    41881 gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta
    41941 ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat
    42001 gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt
    42061 tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg
    42121 tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga
    42181 atggggacca aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt
    42241 ggtagcagtt gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac
    42301 taaaaggagt tgcagcgaga ctgtgactcc tttttggctt tatttattta aaataaaaag
    42361 aagttttccg gaaattttta tttaactgtc gtgtacgaag aaaagtttgc tataatggaa
    42421 aaaagcgtgc aaagaaagag ggagtttcat tatgatgcta cgtgtagaaa aattgcgcaa
    42481 aaaaatgcaa gaagaaaatt tggattcatt tttggttacc agtccgtaca atcttcgtta
    42541 cttaacgaac tttacaggta cgacaggatt agctgttatt actttggaaa aagcattttt
    42601 tattactgat tttcgttata ccgagcaggc ggcagcgcag gctcaagggt ttgaaattat
    42661 taaaaatgtc ggacctattt ttgatgaggt agcaaactta gtacgcaaag aagaactgca
    42721 ctcattagct tttgaggaaa caacagtctc atttttagaa tattcggttt tagaagaaat
    42781 catcgatgcg gaattagtac ctgtttcaca gatgatcgaa gaactcagag aagtgaaaga
    42841 tgaagaagaa atcgctatta tcgaaaaagc atgtcatatt gcggatatgg catatgatca
    42901 catcctgaaa atgatccaac cgggcatgac tgagattgaa gtcgcaaatc aactagactt
    42961 ttatatgcgt tctcttggcg cttcaggtgt ttcttttgat acgatcgttg ctagcggatt
    43021 gcgttcggct atgccgcatg gtgtagcaag caaaaaaatc atcgaacaag gtgacttgat
    43081 cacattggac tttggttgct attacgaagg gtacgtttcc gatatgacac gtacattcgc
    43141 tattggtgat cctggggaaa aattaaaaga aatttatcaa atagtcttag atgctcaatt
    43201 agcagttatt gatgctgcaa aaccaggaat gactggtgtc caattagatg cagttgctag
    43261 agattatatt tcaaaacatg gttacgggga agcatttgga cattctacag gacatgggat
    43321 cggtttagag atccacgaag gaccaaacgt ttcttctcgt gcagaaaagc aatttgtacc
    43381 aggaaatgtg atcactgatg aaccagggat ctacttacca ggaatcggtg gagtacgtat
    43441 tgaggatgac ttgttgatca cagaagatgg aaatcgtgtg ttgactcgtt caccaaaaga
    43501 attgatcatc ttatagtttc atcaatagaa gaagtaagcg taaaaatcag tgttcacaga
    43561 ctttgttata gaaaatcact cgatttaatg atacactata gaagaacttt taagctgtaa
    43621 gagataataa agttgggtgt tttctccaat tcatagcagt ttgcagctga tcggcggcag
    43681 ttttcttacc acgtgatcac tatgccgcat catagagaat tcggatgtat ctcgtgctta
    43741 ggcaaaggga ggaataaata aatggcagat gaaaaaaatt tgattttaaa cgcaaaccaa
    43801 gatttaggag agatcgtgat cgctcctgaa gtcatcgaag taattatcgg gattgcagct
    43861 tctaaagtag aaggcgtata cggaatgcgc ggaacttttg ccagcaatgt cactgaatta
    43921 ttaggacgtg cagctcacgg aaaaggtgtt tacttagcaa acgaggaaga tggcttgaaa
    43981 gtagatcttt actgttatct agaatatggt gtttcagttc ctaaagtagc aatggaaatg
    44041 caggaacggg tcagccagca agtccttttc atgacggata tcgatttatc tgaagtcaat
    44101 atccatgtag tagctgttgt tcctgaaaaa ttagaacaac caacaatcga agaacttttt
    44161 gaagacgaag aggaagagaa tgaataaaga attatcaaga cacgagattc gggaaatggc
    44221 attgcaggct ttatttccat tagacttcaa tgcagaccta acaaaagaag atgcgatttt
    44281 caacgcaatc gaattagacc accgcgacat gatcaacgaa gatgaatcag agtttgttcc
    44341 tgtttatctt gatacgctag ttggcggtgt ctgtgcaaaa aaagatgaat tggataaagt
    44401 gattgaaaaa catttaaaaa acaattggca catcaatcgg atctcaaaaa tggatttggt
    44461 gatcttgcgt atcgcgattt ttgaaatgac ttatgtgaca gatgtaccag cggctgttgc
    44521 tttaaatgaa gcaatcgagt tggcaaaaac atttagtgat gaccgttcac gtaaattcgt
    44581 caacggtgtt ttatccaatg tattgaaaga attagaagct ggggcgtaag tcctagcttt
    44641 tttctatgaa tcatcataaa cctgttataa atcgggaaaa ttctatggta gaatgaaaac
    44701 acaaaaaata tctgggagtg gttagatggc agaattgatt aacggaaaag aattagctga
    44761 aaaaatgcag gcagagattg ctgaaaaaat ccaaaaattg aaagataatg gtatccatcc
    44821 cggattagtc gttttattgg taggggaaaa tccagccagc caaatctatg taagaaataa
    44881 agaacgtgta gcaaaagaaa tcggaatcca ttcattggta gagcgttatc ctgagacgat
    44941 ttctgaagac gaactacttg cagaaattga aaaatacaat caagatccta gttttcatgg
    45001 tatattggtc caattaccat tgccaaagca tatagatgaa gaaaagatcc tgttagctat
    45061 ccgccctgaa aaagacgtag acggttttca tccgatgaat ttaggcagac tccttgcggg
    45121 taaaccggac aagattcctt gtactccata cggcatcatg aaaatgtttg aagcttattc
    45181 tatcgatccg gcgggtaaac gagcagtagt tatcggacga agcaatatcg tcggtaaacc
    45241 gatggctcag ctactcttga tggcagatgc aactgttact gttgctcatt ctaaaaccga
    45301 aaatctggct gaactggcac aagaagcaga tattttagtc gtagcaatag gacgtggaca
    45361 ttttgtcaca aaagaattcg taaaaccagg tgctgtagtg atagatgtag ggatgaatcg
    45421 tgatgctaac ggtaagctga tcggtgatgt gaaatttgat gaagtggagc ctattgcagg
    45481 ctggatcact cctgtgccaa agggtgtagg gccaatgact atcacgatgc tgatgtatca
    45541 aaccgtaaaa tctgccgaag cttttggagc aggtgaatag atggcacaag aatatttaac
    45601 tgtcactaca ttaacgaagt atttgaaaag aaaatttgat gccgatcctt atttagaacg
    45661 cgtatattta acaggcgaaa tatcgaattt ccgtttacgt gccaatgccc atcaatattt
    45721 cagcttaaaa gatgaccatg caaaaatatc tgctattatg ttcaaaggag cattccaaaa
    45781 attacgattc cagccaaaag aaggaatgaa agtccttgtc gttgggcgta tttcattata
    45841 tgaagcggga ggttcttatc aaatctatgt agagcacatg gagcctgatg gaataggtgc
    45901 tttatatcaa gaattggaag aacgaaaaga aaagctgatg aaggaaggcc tttttcaagg
    45961 gcctaaaaaa atgctgccac gtttcccaaa gcgaatcgcg gtccttacca gtccaagcgg
    46021 agcggttatc cgcgatatca tcacgacggt gaaaagaaga tatccaattg tccaattggt
    46081 tttgttccca acagttgtac aaggaaacca agcagcggac aatgttgtca ggaatatcca
    46141 gagagtagaa gagatgggca atttcgatac aatgatcatc ggtcgtgggg gtggttcgat
    46201 tgaggattta tggccgttta atgaagagcg ggttgctcgg gcgatttatc aagctaatac
    46261 gccggtgatt tcatctgtag gacatgaaac agataccacg atcgctgatt tagtagcaga
    46321 tgttcgggct gccactccaa cagcagctgc agaattagct gttcctgttt tgtcagaaga
    46381 gttgatgcgc attcaagaac ggcaagctag attgcagcag gcgtttgtac gccagatcca
    46441 gcgcaaacaa gaacggttcg agcgtgctcg acgttcctat gtatttcggc agcctgaaag
    46501 attgtatgaa ggtcagacca tcaaattaga ccagttgaaa cagcgattat atcaagctac
    46561 acagcaaatt tatcatgcga aagaaaaaca aacgatcgca ttaagccacc aattggaaca
    46621 agtggctccg ctttatcgag ttaaagcagc tcgccaagaa actgcttatc tagaaaaacg
    46681 cttagaagag aaaatccaac aatatatgaa acaacagcag cagcgttttg ctcaagctgt
    46741 tcaatctctt gatttgttga gtccattgaa aatcatggga agaggctaca gctatacgac
    46801 gttaaatgat caagtagtaa aaagtgtcac cgagattcat caaggggatt tgctgacgat
    46861 ccattatcaa gatggaatcg ttaaaggaca agtacaagag atagacaaag gagaataaaa
    46921 atatggaaaa tccaactttt gaagagtcac tgcaagaatt agaaaaaatt gtcatgcagt
    46981 tagaacaagg agacgttcca ttagaatcag ctctagattc gtttaaacga ggaatggaat
    47041 taagcaaaca ttgccaagat acattagcta aagcagaaga gaccttgacg aaaatgatga
    47101 cagaatcaaa tgaagaagtt gattttgacg gaaacgagga aagctgagat gagattaact
    47161 gacttttcag ctattcatct acctgctgta gaaaaagaaa tcttgtcttt tttagatgag
    47221 catacaacgg atagaggact ctatgatgcg atgagttatt cagtgaaagc aggcgggaaa
    47281 agaatacgtc ccttattact tttaacggcc gttgcttcct ttgatgagcc tattgatgtt
    47341 cccgtctatc aagtagcagc tgctttagaa atggtgcata cgtattcctt gatccacgat
    47401 gatttgcctg cgatggacaa tgatgatctt cgtagaggaa aacctaccaa tcataaagta
    47461 ttcggcgaag cattagctat attggccgga gatggtttgc ttacaggagc attccagcta
    47521 atcagtatgg cacacttagg taattctcct aagcttctgc tgcttcagca attagctgtt
    47581 tgtgcgggat cacaaggtat ggttgctggg caagctgcag atatcgaggg cgaatcgaaa
    47641 aagctttctt tagaagaatt agcatttatt catgaaagaa agacagggca tttgattcgt
    47701 tatgccttgc tggctggtgg catattagcg aagcaacctg aagaaatctt attgctactt
    47761 caacgattag ccgaacattt aggtttagct tttcagataa gagatgatct actcgatgtg
    47821 attggtacaa cgaaaacatt agggaaaaca gctggtaaag atgaacgtat ggaaaaaaat
    47881 acttacccgc gtttattagg cctagaaaag acacgtgagg cgttggagat tgagctgtta
    47941 tccgccaaca agatcataga caaattagaa gaggacgttt tttcttttga tggcgggctg
    48001 atgcgccaga tgatcaagca atttgatgtg gagaaatgag ggaaagaatg gaaaaagaac
    48061 gcgtagatgt attagcggtc aaacaaggat tatttgaaac aagagaacaa gccaaacgtt
    48121 cagtgatggc tggtttgatt tacaacgaaa aaaacgagcg gtttgataaa ccgggagaaa
    48181 aaattccagt gagtagtgaa ttaagagtca aaggtaaaaa acttccttat gtttctcgag
    48241 ggggattaaa attagaaaaa gcactcaaag catttgatct atctgttgaa ggaaaaacat
    48301 tgttagatat tggtgcatcc actggcggat ttactgatgc agctttgcaa aatggcgcaa
    48361 aaatgagtta tgctttagac gttggctaca atcagctagc ttggaaaata agacaagatc
    48421 ctagagtcgt cgtaatggaa agagtgaatt ttcgttatgc aaaacccgaa gactttacct
    48481 taggaattcc agaagttgct gttatcgatg tttcttttat ttcactaaaa ctgatgctgc
    48541 ctccattaca tgctatttta aaagaagagg gagaagtgat tgctttaatc aaaccccaat
    48601 tcgaagcagg cagagaagct gtagggaaaa acggaattgt tcgtgaccct cagacccatc
    48661 aaaaagtttt agaagacatt ttgtcatttg cggcacaagg tggatatgat gtgctggaat
    48721 taagttattc tccgatcacg gggggagaag gaaatatcga gttcttggct cacttgagga
    48781 aagtacccga aagcggaaca atcaattcag ctattaatat ggcagaagtg gtttcaaatg
    48841 cacacgaaca gtttgaccat aaataaattg taactgtgaa aatcaagtga gaagtatgcg
    48901 gattcttctt tattatacca aggaaataag ctatcattga ttaagaagaa tatgctaagg
    48961 agcgaagacg taatgagaaa aaaagatcgg catcgtttga ttactcgatt gttgagtgag
    49021 caagatatcc gaaagcagga agaatttgtt gaaatcctga aaagcaaagg aatatctgtt
    49081 actcaagcta cgatttccag agatataaag gaactgaaac tgatcaaggt accggcttca
    49141 gatggcggct accgttatag cataccagct gaaacaaacg aagatgtaaa tactaaattg
    49201 gaaaaactat taaaagacgc tttcgtggca gttgaccaaa tggaaaaatt tgttattttg
    49261 aaaacattgc cgggaaatgc ttcggcagca gcaaatttga ttgataaacg ttacaagaaa
    49321 gaattatttt cgattatcaa tgatgacgat aatgttttga tgattacacg aactgaagac
    49381 caggcagttt tattgaagaa agacttcctg cattaccttt aagttgagga gcgaatgtga
    49441 tgctacaaga aatcagtatt actaactttg cgattattcc ggaattgcgg ctttcttttc
    49501 acgaaggaat gaccgcgctg accggagaga ctggtgcggg taaatccatt attatcgatg
    49561 ctttaggtct gttagcagga ggaagaggtt caagcgatta tatacgtcaa ggagcagaaa
    49621 aatgtgtgct tgaagggttg tttgaactac caaaacaaga aggattctct gaactgatgg
    49681 ttgaattagg gatcgaaaca gatgaggaca atctgattgt tcgacgggat atgtccttaa
    49741 ctggaaaaaa tgtttgtcga gtgaatggtc atattatcac attagctaat ctgagaagaa
    49801 tcggcagtta tttagtagat atccaaggac aaaacgaaca tcaggaactg ctccagcctg
    49861 aatcacatct tgctttattg gatcgttttg gagatgtagc tttccaacag aagaaaaaga
    49921 gttatcagca agaatatatc tcttatcgtg agctggaaaa aagggtacga aaagtgcagc
    49981 aaaatgaaaa atcctatgtt cagagaatag atatgctcca tttccagcaa gaagaaatag
    50041 ctgctgctga tttgcaagtc ggagaagaag agaaattaaa agaagaaaga gaaaaactta
    50101 gcaattatca aaaaattgct gatggattag ctgcaggtta cggagcactg acgaataatg
    50161 aacaaaacag tgtagatggt gttggcttgg ccgtttcaga actgcaaggg atcgcccatc
    50221 tagatgtgga gtatgaagca atttatgaaa atatccaaag tgcttactat ttgctgcaag
    50281 atgcaatcgg cgatatgagt cgtcagattg atttattgga gttagatgag agtcgtttag
    50341 aagaagttac gcaacgttta gaattaatcc gacagttgaa acgtaaatac ggcgaatcca
    50401 ttgagtcaat attggcttac tatgacgaaa tcaccgaaga gctagcttct tctgattttt
    50461 cagaaggtca attggacaaa atgaaagagg aactagagca aaaagagctt ctacttcagc
    50521 aacaagccgc agatcttcat gaagcgcgta aaaaaatcgc aaaagaattg gaaaaatcca
    50581 ttctgcatga attgaaaagt ctgtatatgg aaaacactga gtttgaggta cgtttcttaa
    50641 aagaagaaaa tagacaattg aatcatgatg gatttgacca aatcgaattt tacattacga
    50701 ccaatccagg agagccactg aagcctttgg tgaaagtcgc ttctggtggc gaactgtctc
    50761 ggatgttgct tgctttgaaa actatttttt cttctgagca aggtgtgaca agtattattt
    50821 ttgatgaagt ggatacaggt gtgagcggaa gagttgcaca ggccatcgca gataaaattt
    50881 ctcaaatatc gaaatactct caagttttgt gcatcactca tctgcctcaa gttgctgcag
    50941 tcgccgacta tcaatattat attgtgaaag cagtgattga tggacgtacg caaacttctg
    51001 tttcagagtt gaaaacaaaa gaaagagaag aagaaattgc acgtatgctg gctggaagtg
    51061 agattacaaa actcacactc gaacatgcaa aagaactatt gaaactcgca aaaaggacct
    51121 gagccacctc gcagtggttc aggtcctttt cagcagctga tagctatatt ttatttttac
    51181 atgacactca ttacagcagt caatacaaca gatccaatcg ttaaaatcag cattgtccaa
    51241 atgacaaatt ttgttacttt agcaaaagta cttgtttgtt ttttttcacg cattgttttc
    51301 aacctcgtca ttaattagtt gattcaagtg tacaatcttt tttccatttc cacaagaggg
    51361 cttttttata aaaacttccc tttttttaaa taataggtag ttacgataca aataaataat
    51421 gttaatagaa ctagccatat aaaggcatca tccctttcag caataggtaa cttaatgttc
    51481 attccataga tactgccgac gattgtcggt atagttaaaa taatcgttaa agaagttaaa
    51541 attttcataa tattattcag attattcgat acgattgctg aaaaagtctg attcatttgt
    51601 tctactagtt ttaattgaat gcgagaagta gtcattgcct gctctgtttc caccaataca
    51661 tcacgtaaat gatttttcaa agcatgattt tcttggaaaa gtttattttg cgctagttct
    51721 ttcaatgcat ctaaattagc agaagtcgct tctctgaaat aaacgaggct tttttggata
    51781 tccatgattt gataaagttg tttgttttca gtggatactt gaagtttggc ttccaaagta
    51841 tcgcattgag attgaagcgt agctaaataa ctgttgtaag agagtgtgac ctgccaaatg
    51901 aattgaaaga agatattgag attaggcgaa atatcacttt ttggaaaggt ctggctaaag
    51961 actttcttta aaaacagtgg ttcatgattc gtaattgtga taagcttctt tttgggcgtc
    52021 aaaataatag agagcgggta tgtatccact tgaaaataac cgctaggact tgttttagca
    52081 aaaggatatt gtaacaataa taacgctggc tttgtcaact ttgattgatt gaattcttct
    52141 gtacgggaat tttctgcatc gtccaatatg gaagtgatgt aatctgacgg aatttcaaat
    52201 tgatgtgtca gctgatgaat ctcttcttga ttaggacgtt ctacacaata ccagatcgtt
    52261 tggtcagttt cctttccggt attacgcagt ttctcattat ctaatgtaaa atatttgatc
    52321 atgattttcc tcctgttgcc tgtttctttt attataacgg atagggaatt ggaatacatg
    52381 gaatgtgttt gtaagatata gaatataact gcataaattt aaataaaatg aaacagttgt
    52441 gtaaagtata tacagtatac ttgaagcaac gataaaaaag gagtttacgt tcatgggcac
    52501 attcacacca cttatcttgt tttggtatat ttacccggta atcgttttgt ttggttgcca
    52561 atttcttgta aaattatttt cgttaaatcg tcgttttaaa ataaaagccc ctgatttggc
    52621 gattcctttt ttgtggggcg gtatgcatgc attgtctaga aatacaggga caatttctat
    52681 cttaccgttt ttctttattt ctgttttgct aatgggaatt tgtattgctg ttttccaagc
    52741 atattattat gaagaaatta tttatccgcg ttatttcaag atgacttggc gtttggtttt
    52801 tctgttgaca atcgtcttgt acgctgtctt gatcattgtg aacatacttt cttacctatg
    52861 aaaaaaatag aaaagctgta aaaagtcttt taaaaggctt tttgcagctt gatttttaaa
    52921 aaaatgttta tgaataaaaa aagttgttcc cggatttccc agaaaaggtg gtagaaagtg
    52981 ggggattgtg gtagactaat tttagttagt ggaggaatgg ggggctttca atgttgatgg
    53041 gtgaattcca acataatatt gatgcaaaag gacgtttaat cgtgccctca aaacttcgtg
    53101 aagaacttgg cgaaaagttt gttctgacac gcggattgga tggctgtctt ttcggctatc
    53161 cgatgagcga atgggaaaat ctggaagcta aactgaacga aatgccgttg gctaaaaaag
    53221 acgcacgtac ttttgtgcga ttcttctatt cggctgccac tgaatgcgaa cttgataaac
    53281 aaggacgaat caatataccc agcacgttaa gaaattatgc agctttgaca aaaggttgcg
    53341 tagtcatcgg tgtatctaac cgaattgaaa tttgggatga gacacgatgg caagattttt
    53401 ctgcagcagc agaagagaac tttgacgaaa ttgctgaaag tatgattgat ttcggactat
    53461 aagaatagga agaggctgac taatgactga aacatttcaa cactatacag tcatgttgaa
    53521 agaaacggta gatggattga acatcaaacc ggatggcgtt tatgtagact gtacactcgg
    53581 cggtgccgga catagtgaat atttattaag ccaactaagt tctaaagggc atctgtatgc
    53641 gtttgaccaa gatcaaaaag caatcgatca cgccaagctg cgtttagctt cttatgtcga
    53701 acaaggtatg gtcacattta tcaaagcgaa cttcagagaa ttagaagaac gcttgagtga
    53761 gcatgtagat aaggtagacg gcattttgta tgatctaggg gtttcctctc ctcagctaga
    53821 tgaagcagaa cgtggattta gttatcatca agatgcaccg ttggatatgc gcatggatca
    53881 gtcagctcct ttatccgctt atcacgtaat taatgaatat tcctaccatg aattagtaaa
    53941 gattttcttc cgctatggcg aggagaaatt ttccaaacaa attgctcgtg aaatcgaacg
    54001 agtacgaaaa acaaaaccaa tccaaacaac tggtgagctt gtcgaaatca ttaaacaagt
    54061 catcccagct cccgcaagaa gaaaaggcgg gcatcctgca aaaagaattt ttcaagctgt
    54121 gcggattgca gtaaatgacg aactcggtgc cgaagaggcc tctttagaac aggcaatccg
    54181 gttattgaaa gtcaatggaa gaatcagcgt aatcacattc cattcattgg aagacagaat
    54241 tgtcaaaagt atgtttaaag aatatagtac ggtacaagat ctaccaccgg gacttcctgt
    54301 catacctgac gaattccaac cagaactgaa agtcattact cgtaaaccaa tccttccttc
    54361 agaggaagag ctaacagaaa acaatcgatc acgcagtgca aagttacgta ttgcagaaaa
    54421 aataagaatg aaagaggagt agaatgatgg cagaattaaa gaaaatgcaa gattacgaat
    54481 atgatttacc tgaattggtg gatcagccgg aacaacaaat gcctgattct tcttcgaatc
    54541 aagaaatcat tcttccacaa tcaccggcaa aacgattaaa gcagatttct gctttggaaa
    54601 aaattgctgt ggcaagcgtg gcgattgcta ttgttgcttt atgtgtttta acagttatgc
    54661 tgcgcacaaa tatcagcgga gctgaaaaag aaatcacgtc aatacaaaca gaaatcaatg
    54721 ataaaaagca agaaaaaacg agtttagaac aagaaaaaaa cgaactttct cgtactgaac
    54781 ggattaaaca aatcgcagaa gaaaaaggat tgtctattaa tgacgacaat ttaaggaaag
    54841 tgaagtaaat gagtcttaaa aataaaattc ggcggtttat ggaaaagaaa aatctaaatc
    54901 cgatgaacaa tcgcaaaaaa gtaggtatca ttttatttgc tacgagtatt ggattgttct
    54961 ttttatttgc cgtccgtttc tcgtatatcg ttattggagg tcatgttgct ggtacctcat
    55021 tagctgaaaa aacaaaacag ttgtaccaag gaagtgaagt agtaaaagca aaaagaggaa
    55081 cgatttatga tcgtaacggt gtggcgctcg cagaagatgc ctcctcttat tcaattaaag
    55141 cgatactctc aaaaacatat acctctgggg ataaaaaact gtatgttgaa gaaaagaatt
    55201 ttgataaaat agcagaaatc ctgcataaga atctttctat agacaaaaaa gatgcactga
    55261 atattttaga ggacggcgca aaaaaagagc tgtatcaagt tgaatttggt agctatggga
    55321 aaaatatctc ccaagaaacc aagcaaaaca tcgaagcgga catgaaaaaa gaaggtgtcg
    55381 ctggtcttta ttttgtcgac catcaggctc ggatgtatcc aaatggcgtc ttttcttctc
    55441 attttattgg atacgcagtt ccagacaaag atgagaatgg cttagttggt aaactaggtt
    55501 tggagtctgc ttacaatgat atcttgagcg gtaaagatgg taagattatt tatcaaaaag
    55561 ataattttca aaatcctctg ccaggtacag tggcagaaga agaaaaagct gtcgacggac
    55621 aagatatcta cacgacttta gacagcagac tacaaagtta tttagaaaca ctgatggatc
    55681 aagtaaatga agaatatcag ccagaagaat tgacagcagt cctgatgaaa gcaaaaaccg
    55741 gagaaatctt ggcaatggga caacgaccta ctttcaatcc tgaaacaatg gaaggattga
    55801 ctggaaaaga tgctatctgg cgtaatttct tggtacaaga tagttatgaa ccaggatcaa
    55861 ccatgaaagt tttcactact gcagctgcta tcgaagaagg agaattcaac gagaatgaaa
    55921 ccttccaatc tggaaaaatc caagtagaag atgctacgat caatgaccat gactttggtg
    55981 aaaaaggtgt gctgaccatg cgtcaagctc tttcatggtc aagtaacgtc gggatggtca
    56041 tactggaaca gcgattaggc ggacgatggt ataactattt acaaaaactg ggatttggac
    56101 aaagtactca ttctggatta gatgatgaag taaacggtgc tttacctaca ttaaatattg
    56161 tcgaccgagc aatgagtgct tatggacaag cggtaggtgt gacgaatttc caaatgatga
    56221 aagctttcac ttctattgcg aataacggta caatgattca acctcgatat atcagcaaag
    56281 tggtggatcc ccaaactgga gaagagcgaa caactcaaac agaagttcta gggcaacctt
    56341 tttcaaaaga aacaacagaa aaagtccgtg aatatatgag agatgttgtc gaaagcgaaa
    56401 actacggtag tgcttatggt gtatatagtg tacctggata caatgtatcg gcaaaaaacg
    56461 gaacagccca aatcgcttct gatacagggg gatatcaaac aggagataca gcctacctgt
    56521 attcgattgt ggagatggtt ccttctgaag atccagacta tgtcttgtat ctaacaatga
    56581 aacaccctaa gacatatgat cgaatggcat tggctaaaat cgcaaatcca ttgatgaaac
    56641 gggcaatgga ttttaaagaa actgaagaag attcagatac agaaacaaaa acagaaaaag
    56701 tatccgtagc cgattatcgt aatcttgaag cggacgttgc agcagctgat gctcaaaaaa
    56761 gtggactgca acctgtagtg atcggtaatg gtaaaaaagt acaaaaacaa tctactgcca
    56821 acggtgatca gttgatctct ggagaaaaac tgatcctata tactggtggt gataagctga
    56881 tgcctgacgt aacaggatgg tcaaaagcag atatcatgaa gctggggaaa atcctcggta
    56941 ttgaagttag ttttgatgga gatggttatt gtgtcaaaca agaacttgct ccatatgaaa
    57001 aaatcactga agacaaactt agttttactt tagaagaata agaagagaaa taggagagaa
    57061 aagagcatgg aatggacaca agcattgatt cccattgtca gcagttgtgc aatgacaatt
    57121 gccgcaatgc cgctatttat tggttatttt caaatgaaaa agcaaggtca ggcgattcgt
    57181 gaggagggac caaagtggca caatagcaaa gccggaactc ctacaatggg cggcttagtg
    57241 tttctgatcg gctctatttt gacaggaatt tgggttggag cttggcaaaa acagctaact
    57301 cctactcttt tcatcttgct ttttgtatta gcgttatacg gagtaatcgg ctttcttgat
    57361 gattttataa aaatattcaa gaaaagaaac atgggactga actcgaagca gaaattgatt
    57421 ggacagattc tagggggaat catcttttat cttgtttacc gttctgaagg ttatccaggt
    57481 gtattgaatt tcttcggact agatctacct ttaggaatca tttatggtat tttcgctctc
    57541 ttctggcttg ttggattttc aaatgcagta aatctgacag atggcatcga tggattagtt
    57601 gctggcttag gaagtatctc ttttgcagct tacggtctaa ttgcttggca tcagcatcag
    57661 tatgatgtat taatcatttg cttaagtgtt ttaggaggtt tactgggttt cttcgtctac
    57721 aacagaaaac ctgccaagat ttttatggga gatgtcggtt cattagcgtt gggcggactt
    57781 ttagcggcaa tttctataat gctaaaccaa gaatggacat tgcttttgat tggtttgatc
    57841 tatgttatgg aaacagcaag tgtgatgctt caagttactt cctttaagct tacaggaaaa
    57901 cgaattttta aaatgtctcc aattcatcat cactttgaaa tgtgcggatg gtcggagtgg
    57961 aagattgata cagttttttg gttgatcagc attgtgactt cgttgatcac attatggatc
    58021 gtttggtagg agatgacggg aaatgaaaaa aatatcaaca tatgaaaata agaaagtgct
    58081 tgtcttagga ctcgcaaaaa gcggagtaag tgcggccaaa ttattacatg aattgggcgc
    58141 acttgtcacc gttaatgatg gaaaaccttt tgacgaaaat ccggaagcac aagagctgct
    58201 ttctttaggg ataaaagtaa taacaggtag tcatccaatc gaattgttgg atgaagaatt
    58261 ttcattgatg gtcaaaaatc cagggattcc ctacagtcat cctttagtag caaaagccca
    58321 agaaatgggt attccggtaa ttacggaagt tgagctagct tacgaagtag cggaatgccc
    58381 aattattggg attacaggaa cgaacggaaa aacaacgaca acaacgatga caggactact
    58441 actgaatgca ggagcagatc aaggaattgc acgtttagcg ggtaatattg ggtatccagc
    58501 aagtggtgta gcgcaagaag caaaatcaga agataaaatt gtgatggaat tgtctagctt
    58561 ccaactcatg ggaatcacag atttccgccc tcatatagcg gtgattacca atatctatga
    58621 agcacatatt gattatcatg gtacaagaaa agaatatgtc aaagctaaat ggaaccttca
    58681 aaaaaacatg acagaaaaag attatctgat ccttaactgg aatcagtcag aactacaaga
    58741 attagcgcaa agaacaaaag cacgcgtact gcctttctcg acaaaagaag tgttggaaga
    58801 cggtgtgtat gctgacgatt actcaatata ctataaaaaa gaaaaaataa tggaaatatc
    58861 tgaacttggc gtaccaggta agcataatgt agaaaatgca ctagctgcta tttctgtagc
    58921 aaaattgtat ggtatatcaa atgaagctat acgagaaacg ttgcattttt tccatggcgt
    58981 accacatcgt acgcaatatg taggagaaat ccaaggcaga aaattttata atgattcaaa
    59041 agcaaccaat attttagcga ctaaaatggc tctaagtgga tttgaaacgt ctaaggtcgt
    59101 gttattggct ggcggattag acagaggaaa tacatttgat gaattaattc cttcattaaa
    59161 aggaatcaaa gcaatggtcg tatttggcca aacaaaagaa aagttgatgg atgctggaaa
    59221 aaaagcaggc atcgagacaa tagtaacagc cgattctgta gaacaagcag ttcctttagc
    59281 attagaaaac agtacagatg gtgatgtagt tttattatca ccagcgaatg ccagctggga
    59341 tcaataccca aattttgaaa ctagaggaaa cagattcatg gaagctgtga accgattaaa
    59401 atagaaaaag tagaagcgag cgataaaaaa tgaaaatttt ggttacaggc ggaggaacag
    59461 ggggacatat ttatccagct cttgcctttg tcaattatgt aaagactcaa gaaccgaatg
    59521 ctgaattcat gtatgtggga gcaaaacgag gtctagaaaa caaaatcgtc cctgatacag
    59581 gaatgccttt ccataccttg gagattcaag gatttaaacg aaaaatttcc atgcataaca
    59641 tcaagacggt ccgattgttt cttaagagta tccgtcaagc aaaaaaaatc ctcagtgatt
    59701 ttcaaccaga tatcgttatc ggtactggag gatatgtttc cggagcggta gtgtacgctg
    59761 cagccaaaat gggtattcca acagttatcc atgaacaaaa cagtgtacca ggtgttacca
    59821 ataaattttt aagccgatat gtggatcgga tcgctctttc atttaaagat gcggcttcct
    59881 tttttcctga aaacaaagca gttcttgtag gtaatcctcg agcacaagaa gtagcagata
    59941 ctaaaaaatc agatattttg aaaacatttg atttggatcc agaaaagaaa acagtcttga
    60001 tttttggcgg tagtcaagga gcacttaaaa tcaatcaagc agtatcagtt tttttaagtg
    60061 aatatattcc ttcttcttac caagttttgt atgcttcagg agatagatat tatgaagaga
    60121 tcctaaataa aataaatcct gaaatacaaa atattagtgt ccagccttat atcaaaaata
    60181 tggcagaggt catggcttcc agtgatctgc tggtggggcg tgctggtgca acttcgatcg
    60241 cagaattcac agctctgggg ttaccggcag tgttggttcc cagcccatat gttacgaatg
    60301 atcatcagac taaaaatgca atgagtcttg ttcacgcagg agcagcaaaa atgattgcag
    60361 ataatgaact aacaggtgaa tcactaagtc agacagtcaa cgaaatcatg ggtgatgaag
    60421 aattgcaaaa gcaaatgtgc cgagcttcta aagaacaagg aatccctgat gcttcaaaac
    60481 gtctttatga cttagtaaaa caaataatca aaaaataaag gcggtggtct tagtgagtaa
    60541 gaaaaaagaa ccaaaaaaat tgtctcatcc gtcttctgat gaaaacaatt tgactccttg
    60601 gcaaaaggct aaccgaaaat atctggcaga acaagagatg aaaaaccaag agccaaaaaa
    60661 agaagacata acattagata cacccctttc agattctgaa gaagaaaaaa cggttcaaga
    60721 ggaacagcaa gaagaaagcc agtccgagaa aaaaatcgaa ttttttgaag agatcaagca
    60781 aaagaaaaac ggtggaccat ataacggttc ttttttgaac cggctgccca atttaaaaag
    60841 tcaacggcac aaagtactat ttcgtcggtt agctttgatc atcactgtat tgggtatacc
    60901 gctgattttt ttgatctatt atgtttctcc ttatagcaaa ttacaagcag ttgcagtatc
    60961 aggcaataag acagtcaaca gccaagaaat catctctgat acaaaattgt cattaggaga
    61021 aaatgtttgg ggacagtatt ttcatcgatc tacttatatc gatcgcctta aaaaagcaca
    61081 gccaagaatc gaaacagcaa atatacattt caaagggatg aatgaattcg atttggatgt
    61141 aacagaatat aaagagattg ctttgatcgc aaaaaacaat caatactacc cagtgattga
    61201 aaatggaaca gtattagatg aaaaagtagc aaatcctaca aaaaatctcc ctatcttgga
    61261 agagttcaaa gactctgcaa aaatcaaaga attaacaaag caatacaatc agttatcatc
    61321 agaattacaa aaagcaattt ctgaaatcaa gtacacgcca agagcaagca ataaaaatct
    61381 gatccaattg aatatgaatg acggcaatca agttattgta aatatcaata atcttgcgaa
    61441 ccaaatgaaa tattattcgc aagtagccaa agatatggat gaaaaaggtg tgatcgacat
    61501 ggaagttggt atcttctcgc atccttacgg tagcactcaa gaaacggaag aatcgggcca
    61561 atcggaagaa acaactgaaa gtgaagaaaa tgcggatcaa acaaacgaat caagtgaaga
    61621 ggcagaaacg caaaaagcac aaacacctgg atcgtctagc acaaacggat catctgattc
    61681 aaatgattca agtgaaacaa gtcaaataga agataatcca agtacaactc cgtcaagttt
    61741 aaatgaaaat tgataaaaca actttaaaaa gtaagaatgt actttctaaa aaccagtaat
    61801 ttatggtaga attggaagca gtgaatatat ctattaataa gtatatttta aaacggacgc
    61861 tagttgttcg cttgtatatg gaatagtaag taggagggaa tcccattcat ggcaaaaaca
    61921 ggaatgtatg taggccttga tattggaacg acatctgtta aagttgtcgt ggctgaatat
    61981 atcgacagcc aaatgaatat tattggagta ggaaacgcaa aatcagaagg gattaaccga
    62041 ggcattatcg ttgatatcga taaaacggtc caagcgatac aacgagcagt acgacaagca
    62101 gaagaaaaag caggtatcca gatcaaaggc gtaagtgtcg gtttgccagc aaacatgctt
    62161 gaagtagaaa attgccaggg aatgattgca gttaacggag attcaaagga aatcaccgat
    62221 gaagatgtca gaaatgtggc ttcagctgcc cttgttcgtt caatacctcc tgaacgtcaa
    62281 atcgtgtcaa tattgccaca agattttact gtcgatgggt tcgagggaat caaagatcct
    62341 cgtggaatga tcggcgtaag attagaaatg tacggtctgt tgtttacagg acctaaaacg
    62401 atcatccata acatacgtaa atgcgtggaa aatgccggat taatcgtcaa cgaaatggtg
    62461 attactcctt tagcgctgac tgaatcaatt ctttctgacg gagaaaaaga ttttggtacg
    62521 atcgtcattg atatgggtgg cggccaaaca acaacggctg ttatgcatga taagcaattg
    62581 aagtttacca atcttgacca agaaggtggc gaatttgtca ctaaagatat ctctattgta
    62641 ttaaatacgt catttaacaa tgcagaagcg ttgaaaataa actacgggga tgcataccca
    62701 gaacggacat caccagatga agaatttcca gtagatgtga ttggtcagtc agaaccagtg
    62761 aaagtcgatg aacgctactt gtcagaaatt atttcagctc gaatggaaca gattttcaac
    62821 aaagcaaaag atgcattgga ccaaatcgaa gcattagagt tgccaggtgg cgtcgtattg
    62881 acaggcggag ctgctagtct tcctggtgtt gtggacttgg cacaagaaat cttcggcgta
    62941 aatgtaaaat tgtatgttcc aaatcacatg gggctgagaa acccggtatt tacaaatgta
    63001 ataagtatcg tggattattc agcaaacttg agtgaagtgt atcaattggc aaaaattgct
    63061 gttacgggtg aaacctcagc agctcgtcaa atggtagttg aacaagaaac aacaaacaca
    63121 tatgagagct atgaagcacc tgaagaaaca atctacgatg agccggaacc aaaagagtcc
    63181 ggagaaaatg taaaaaataa aatcaagggt ttctttacaa atatttttga ttaattcatc
    63241 ctgggaggaa taagatatgg aattctcaat tgataataat atcaatgacg gcgcagtaat
    63301 caaagtcatc ggagtcggtg gtggcggtgg aaatgctgta aaccgaatga ttgaagaaaa
    63361 cgttaaaggt gttgaattca ttacagccaa cacagacgtg caagcattaa aaaattcaaa
    63421 agctgaaaca gttatccaat taggccccaa atatactcgt ggtttaggtg ctggttctca
    63481 gccagaagtt ggacaaaaag cggcagaaga aagtgaacaa tccttacgcg aagctttaga
    63541 cggtgcagat atgattttca tcactgcagg aatgggtgga ggaaccggta caggtgctgc
    63601 accaatcgtt gcaggtatcg cgaaagaatt gggcgcattg actgttggtg tcgtgacacg
    63661 tccattcact tttgaaggac cgaaacgtgg ccgttttgct gcagaaggta tcgctcgttt
    63721 gaaagaaaat gtggatacat tattaattat ctcaaataac cgtttattag aagttgtaga
    63781 taaaaagaca ccgatgcttg aagctttccg tgaagcagat aatgttttac gacaaggtgt
    63841 acaaggtatc tctgatttga tcacagcgcc aggttacgtt aaccttgact ttgctgacgt
    63901 gaaaacagtg atggaaaacc aaggtacagc attgatgggg atcggtgtgg ctagtggaga
    63961 agatcgcgta gtagaagcaa cgaaaaaagc aatctcctct ccattgcttg aaacatctat
    64021 tgatggagct gaacaagtac ttcttaacat cacaggcggt ttggatatga cattgttcga
    64081 agcacaagat gcttcagata tcgtagcaaa tgcagcaact ggcgatgtaa atatcattct
    64141 aggtacatct atcaatgaag aaatgggcga tgaaatccgt gtgacggtca tcgcaacagg
    64201 tatcgatgaa tcaaaaaagg aacgcaagtc tagccgacca gctagacaag cacaaatgca
    64261 atcacctgct caaaagactg tattagatat ggatcaagct aaaccaactt cttcagaaga
    64321 agaaaacagt tttggcgatt gggatatcag acgtgaacaa aatgttcgtc ctagagttga
    64381 tgattccaat tttgacaata tcgaaaaaaa agaatttgat acattcaacc gcgaagaaac
    64441 aaaatctcat ggcgatgatg aattaaatac gccaccgttt ttccgtcgta aaaaataaag
    64501 gagaggaaga gcacatgatt gctgacaact tgcagaaaat caagcaagaa attcaggatt
    64561 cgtgtgctct tgttcatcgt gatccgaaag aagttacatt agtagctgta acaaaatctg
    64621 ttgaatccgc tcaagctgaa gaattggtta gagctggcgt tatgaattgt gcagaaaacc
    64681 gagtagataa attattagaa aagaaacaag atttatttga ttttcctgaa ataaagtggc
    64741 atttaattgg taatttacag cgcagaaagg taaaattgat tgtaaatgaa attgattact
    64801 ttcatgcgtt ggacagttta agattagcag aagaaatcca gaagcggtta gagaaagaat
    64861 tatcctgttt tattgaagta aatgtttctg gtgaggaatc aaaacatgga atttcaccag
    64921 atgagctttt accgtttgtg gaatctttag ctgcctgcac aaaaatcaag gttgttgggt
    64981 tgatgactat ggcgcctaaa gaagcttctg atgagcaaat ccataaagtg tttggtacgc
    65041 tgaaagaatt gcgtgatctt gttcaagcaa aaggttatgc ccatgcacca tgtagtgaat
    65101 taagtatggg gatgagtcaa gactttcaaa ttgcgattga agaaggtgca acatttatcc
    65161 gtgtaggaac agcgctattc aaagacgaat aaaggaggaa caaaaatgcc gcttttaaac
    65221 agaggagcaa ttgctaattt tttcggatta gctgatgagg aagagtacga ataccaagat
    65281 tatcctgaaa caacaaaaaa ggctgtacag ccaactaaaa aaaatacaac acagaatcaa
    65341 tcaactcatt ctgtatctgc tgcgaatact catgaaaaac aaaaaacggc gacttcacgt
    65401 cctgtaacgc atgaaagtat tcagccggct gaaaagaaaa aagtaattga actacatcaa
    65461 tcacctacaa aaactgtagc gaaagaaaga acagcttcaa gaacctctac acaacctaaa
    65521 gcaaatcttg ctggaaaaat cactgtaatg gaaccaagag cttattctga agctatggcg
    65581 attgctaaac gaatcattgc tggagaagca accttagtga attttcattt agtagaagaa
    65641 aaacaagcga gaagaatcgt tgattttcta acaggaacag tttatgcact agatggagat
    65701 atcaaacgtg ttggagatga gatttttctt tgtacaccga ctgggatgga gattgacagt
    65761 tcaaccgctc aatcttttgc agatgcgaat ttgtttgatc tgtaagagga ggaactacga
    65821 tttatactat tgcactatta cttcatttac ttcaacgggc agcctatttc tatagcatta
    65881 tcttgatggt atatgcattg ctctcttggt ttccaggcgg gtatcaatca gggctaggac
    65941 gattattagc taagatctgc gaaccgtata tcagtttgtt cgatcgtctg cctttgagga
    66001 ttggaccctt ggatttcaca attttagggg caatcctgat cttgaactta gccgtacaag
    66061 gtttagcaca gatcgttata tggataactg tccatttagt ttattgattg aagcaacaaa
    66121 atctttattc tcagaataga taggagggga aaattttgga tgcaaatgta taccagcact
    66181 ttcgggcaga tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag
    66241 gacaatatgc accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa
    66301 cactgattcg acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag
    66361 aaagaaaaag atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa
    66421 cagcattgta tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc
    66481 ttggaacttt agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg
    66541 gtgatcgctg gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa
    66601 caaagattgg aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc
    66661 cgaaagatag ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga
    66721 tctctgagat ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag
    66781 taaaagtgaa ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta
    66841 tttcagtcag aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag
    66901 aaaagtacag ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat
    66961 ggctttaact ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa
    67021 tcaagacgaa gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca
    67081 aagaaatcga gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa
    67141 tgaattaaaa gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt
    67201 aaaaacaagt gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga
    67261 tgagttagtt gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa
    67321 agcaagaaaa atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga
    67381 agatcttaag aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca
    67441 attagaacaa gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt
    67501 gactgacagc catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga
    67561 tactgatgta aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca
    67621 ggctattgat atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact
    67681 ttgttattct taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa
    67741 gatcctctac ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt
    67801 tattggtcac agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca
    67861 tgttcgtccc ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta
    67921 tggttgaatg aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg
    67981 taatttgcca aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca
    68041 aaaaagacaa gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata
    68101 tgccaatggt aatatccatt taggacattc attgaataaa atcagtaaag acattatcat
    68161 tcgttcaaaa tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg
    68221 attgccgatt gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtccatggc
    68281 agaatacttg gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga
    68341 tttcaaacgc ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc
    68401 ttatgaagct gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa
    68461 aggattgaaa ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat
    68521 tgaatataaa gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa
    68581 agatattctt gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc
    68641 agcaaaccaa ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg
    68701 ccgtaagttt gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga
    68761 aaatgtaaca gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca
    68821 tccattctat gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg
    68881 tactggatta gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa
    68941 atataaacta tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc
    69001 tggatttgaa ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga
    69061 aaaaggagct ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac
    69121 gaaaaagcct gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg
    69181 tcagaatatt ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg
    69241 tttgtataat atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg
    69301 agtaccactt ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat
    69361 cgaacatgtg gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc
    69421 gaaagaactt cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa
    69481 agaaaaagat atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg
    69541 tcagcgacca gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg
    69601 tggctggttc aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa
    69661 atcagttatt tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt
    69721 aggaaacatc attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt
    69781 atgggtatct agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca
    69841 agtggcagaa gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga
    69901 agatttcgat cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata
    69961 tatgaccgtt cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata
    70021 taacttcatg catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt
    70081 ctacctggat tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg
    70141 ttgcatgcaa actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat
    70201 cattccgcat acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca
    70261 gttaagcgag tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg
    70321 gactgctttc atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag
    70381 caaattgatt ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg
    70441 tgaaatgtta aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta
    70501 ctttgaagtt atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt
    70561 tgctattaca gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa
    70621 agatgtagga gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga
    70681 agacttctat cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg
    70741 tgtgacatgt tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac
    70801 gataccagtt atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag
    70861 ctgctttagg tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc
    70921 aagcttttcg aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga
    70981 agttcgttcc atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat
    71041 tttcaaccat ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt
    71101 gccctacttc tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg
    71161 ttagaacatt aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata
    71221 cattaacagg aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt
    71281 tgcctgtacg tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc
    71341 ctgcgacgaa tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct
    71401 tgaattgtac accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca
    71461 cttcttcttc gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt
    71521 ctttttcgga aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat
    71581 gattttgcac catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta
    71641 caccgaggtt ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag
    71701 cctcaaaaat attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta
    71761 aataatgatc aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca
    71821 attcaaaagc agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc
    71881 cattttttgt cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag
    71941 gtgccatagc caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt
    72001 cagacaattc ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt
    72061 aaaaatggct tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt
    72121 ctttgacaac ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag
    72181 caaaatgctc gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac
    72241 gctgggctag gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca
    72301 tgtggataca ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac
    72361 ggggattttc tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac
    72421 gatctcatat tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt
    72481 ttcatcaagc actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg
    72541 tcggcgttct tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg
    72601 ttcaggatga ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc
    72661 caatacttca gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa
    72721 aacttcccat aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt
    72781 caattgtaca ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat
    72841 catttcactg acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac
    72901 tttcgttgta tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt
    72961 catattcgat catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg
    73021 ttaattgact atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa
    73081 acgaaagatg atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg
    73141 agggatctat ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt
    73201 ttgttcggga taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata
    73261 ttcatctatt tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc
    73321 agatgtaatc tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata
    73381 ctttgagagt ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga
    73441 gagaacggta atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct
    73501 ttatgagaaa ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa
    73561 agctaaaatt tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat
    73621 aggatacata gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt
    73681 tttttattga ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa
    73741 ttattgcgca tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag
    73801 tggctattgt atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa
    73861 aaaagaaggg tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg
    73921 gtattttaga cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc
    73981 gtgaagtaac ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa
    74041 aagcatttgt tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg
    74101 atgcgcccac actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg
    74161 gttatgccgc ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc
    74221 atgaagaaat gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc
    74281 aaatcctcgc ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc
    74341 catttgatgt agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga
    74401 tcaacttagg tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa
    74461 gcacaaaaaa atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat
    74521 ttggttctca ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata
    74581 ttactcatat ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta
    74641 agcaagcaat tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa
    74701 aagaaatgga tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag
    74761 caagaaatat ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg
    74821 agataatcgg tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag
    74881 atggagaaga accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag
    74941 gtgtttacct tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt
    75001 tcccaagaaa gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga
    75061 attttgatgt atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt
    75121 attttgactc aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc
    75181 atgatcgcgc gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg
    75241 cagatgaagc agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat
    75301 tgttgtatga agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa
    75361 gaaaaaatgg cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt
    75421 tgtacaagta tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc
    75481 ttctcttatt caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt
    75541 ttctcctaca ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga
    75601 accaggtgaa gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt
    75661 agcagatgtt gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg
    75721 cggagccaac gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg
    75781 tacaccagga agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa
    75841 aacaatggta tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga
    75901 tcaaattctt catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga
    75961 tcgttccctt gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac
    76021 agcagaggac gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa
    76081 gaaagatgac tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt
    76141 taatcaactg actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt
    76201 aggaagtctg gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa
    76261 aaagaaaatg gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac
    76321 aggtcttcct tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg
    76381 ttcgggaaga actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa
    76441 taatctaaga gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt
    76501 tttatacgga ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt
    76561 aaataaaaag tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga
    76621 aaaaataaga aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg
    76681 caaataagcg cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg
    76741 aggggttatg tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa
    76801 ggattgacag caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga
    76861 tatttaagtg aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt
    76921 tactctttgc caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa
    76981 gtgacttttg aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca
    77041 aaagctgcga ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt
    77101 acagggaagt cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt
    77161 tctgcagatg cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg
    77221 ctctttggac acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt
    77281 cccggtctaa tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta
    77341 ccgccggaag ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt
    77401 ggagaaagtg cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct
    77461 tcagaaagtt tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat
    77521 cttgcttcac gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa
    77581 gcgaatcgtt tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt
    77641 tatgatgcgg aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa
    77701 tcttttttac attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt
    77761 tcccttcaag tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga
    77821 ttcatggaca gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg
    77881 tcacaagatc cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct
    77941 ctttccgaaa ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat
    78001 gcttatttcc agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg
    78061 ccagatgaaa tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac
    78121 cgtatttata atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat
    78181 cgtgtgagag aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt
    78241 aagagggaat tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt
    78301 gagattcctt ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa
    78361 agtgagcctg ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact
    78421 gcttcaagca tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt
    78481 aatatgccac tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa
    78541 aactatcaag agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac
    78601 accttcagtg atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca
    78661 agtaccatga ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat
    78721 atttatcaaa atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa
    78781 aagtctgaaa ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga
    78841 gtagctgcca aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg
    78901 atccagatgc agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa
    78961 gaatatgaga tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt
    79021 ttttcagcct atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat
    79081 gatgagatcc cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt
    79141 tctattgatg aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg
    79201 tcgattatcg tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta
    79261 gacggattga tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa
    79321 atttatcgtt tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat
    79381 caggtaaaaa ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa
    79441 ataaaaaaat gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat
    79501 aaaaaagagc taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc
    79561 ttgcgttata taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat
    79621 catgttagtt tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc
    79681 agcagaagaa aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa
    79741 taatttagaa tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa
    79801 agctcaattt gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca
    79861 agactatggc atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata
    79921 aatcaagtat gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc
    79981 atggggttaa tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct
    80041 gctaaaatcg gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt
    80101 atacaagcgc atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg
    80161 caagtgacat tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact
    80221 gatttagcaa aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt
    80281 cttttttgaa cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa
    80341 ctctctttat ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga
    80401 tggagaaata catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt
    80461 tgcgtgatgc ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga
    80521 tcaatgcaat tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag
    80581 cagcagacaa aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct
    80641 ggaacggtac actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg
    80701 cccgagctta tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc
    80761 aaggtatttc ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg
    80821 tcgcagatgc aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag
    80881 ttggattatg gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta
    80941 tgatcatggg agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga
    81001 tcaaaatgcc tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag
    81061 cgacgatcgc tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg
    81121 ggcaactgat cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc
    81181 aagggttggg agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta
    81241 ttcatggatc aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa
    81301 tcaatattga tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag
    81361 atgcaatcga agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat
    81421 ggtttggcgg atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac
    81481 gtgcagatta tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg
    81541 aaccagtgat gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga
    81601 ttgctccagt agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc
    81661 cggtatcgca gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag
    81721 gagcggattg gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt
    81781 gggcaccatt tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag
    81841 atgaacgtga ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga
    81901 actgaaatga ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa
    81961 ggatacaatg aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc
    82021 tttgaaacaa gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa
    82081 gcacagcctt ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa
    82141 aaagatagga tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg
    82201 atcgtgttgt atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca
    82261 ggtctttttc taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa
    82321 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    82381 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    82441 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    82501 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    82561 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    82621 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    82681 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    82741 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    82801 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    82861 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    82921 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    82981 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    83041 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    83101 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    83161 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    83221 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    83281 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    83341 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    83401 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    83461 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    83521 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
    83581 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
    83641 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
    83701 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
    83761 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
    83821 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
    83881 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
    83941 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
    84001 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
    84061 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
    84121 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
    84181 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
    84241 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
    84301 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
    84361 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
    84421 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
    84481 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc
    84541 tttgcggtac aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt
    84601 tttatataaa ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg
    84661 aacaatatga agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga
    84721 tacgctaagt atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc
    84781 atgcaaagta aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct
    84841 gacggcgttt gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt
    84901 tttgatttca aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc
    84961 aatcgtatgt ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca
    85021 ttcatttaat ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat
    85081 atcttcccat gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa
    85141 cacgagcata ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga
    85201 tttaaattga tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt
    85261 gattactgct tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa
    85321 cgacgctcta atatggttat taaacttaat aacggtagat tttacatggt ctttagcata
    85381 cttatttaaa aagcgttgat atcccactct atctaaatca gaaatcaata ccgctggcat
    85441 atatttcttt atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt
    85501 tccttcttta taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg
    85561 atccccttta aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc
    85621 tccttttgtc ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta
    85681 tgaaatttcg tattgccaac tatttccacg tttgactaat cttgccataa ttgaatcagc
    85741 tctctttctc tgatacaata ggaactataa agaagcccat tgtttgggtt ttattatttt
    85801 ttgcacatcc tttttgcttg ccggcgcaag gatgtgctat ttgtcgtttt aatggtataa
    85861 ttggatgtgt attagatcat ccaatcagag gtgtttatta tgattaaagt gatttttaaa
    85921 agaataactg aacttccaga gtttagatta tttgttgatt attggtatgt ctggtttttt
    85981 attgttgcat tgctgatagg tctagatgtc atattagaaa gaaaacttaa gtaagagttg
    86041 agttaataaa ttgaattcct aaatgagata ttttgtagcc catattttgt ttgccgacat
    86101 cacctaatac tatggagcta atcgtttttt ctaacagacc aaaatcagat aatctattta
    86161 agctaccatc tattagctct tgtgaatagc cagacatgct gatatttcca gtgaaatttt
    86221 cgtttccacg actcattaag catgctaaca attgaatctc aatcgatgtt aattgattca
    86281 gtacaagaag ataatactcc tcgttatccc atgaagcgtt gttaattcga agtaaacaat
    86341 tcttataaag atttttatac atatctattt tattttgtgc ctttgatttt tctacctcgt
    86401 catttatccc ttcgaatatt ccaatgagtt ggtccttgtt ctctaagttg aaaacatctt
    86461 ctggaatttg atcgcgaatg ttttctaatg attcgtttaa atttttgtag aatttctcga
    86521 tgcgtttgaa acgcttctcg tttttagacc caaaataaag tgactgcaca acgccaccaa
    86581 tataaggaat tgctccaata ccgccttcaa ctattaattc aagatattct tttttggtta
    86641 gtttttcatt gctattatcc atagttacct cctattttgt catttccttt acaagcattg
    86701 tgactcctga tggcactctt ttgtataggt tttattattg tgcacaatct aactcttagt
    86761 ggggcgtggt tcatttattt tttacttact ttactattaa tcatatccca ttgagcatag
    86821 gatttttcta tgttcgatcc tcttgaacta atttctcctg taaaagttac tttatcgccc
    86881 tttgtaaagg tatcagcatt gtttaagtcc ttcgcaaaaa ttacatatga aactttaggt
    86941 gttgcagaaa tatcattcca acttttatca tcgtactctt tgtctgctat aatggctatt
    87001 ctagtagtca ttgattcgat aacagttcct tcaaaagtat atgttttacc actaatatat
    87061 ttagtaaaag ctgtattttg ctcagtagct ggagtaatag aataataact atctaagaaa
    87121 gcaccccagt catgattatt tgtctctaaa aattcttcaa attgagttag gcttttattg
    87181 ctgctgttag atgtagtagt ttgagtatca gatgatgagc tttcctgttt gttattgtca
    87241 tttgcctcgt ttgatgagga aagcgagaaa cctacggtag taaaaataat cccgataata
    87301 aggagaatca taggtgtttt cttatttgtt tttttgataa atcttacaat taacatgatt
    87361 aaacctataa taaataaaac aataccagta aaaccaaata taccgtccat taatagatcc
    87421 tctttctctt ttgatattga atattataaa ccaagcaatt cttttttctt aaaatcgaat
    87481 tcttcttgat taataatccc ttcatctaac aactctttat actttttaat ttcatctgca
    87541 cttgaaattt gtgtttcttt ggaattatta tctaatatag attgttgctt ctctatatat
    87601 ttttttaaat tttccatttc attccaatat ttttttgtaa acataacggt gttttcatct
    87661 ttcgtggccg ctaatatacc tccgcgactt tcgtttccgc ctagcaaggt aaattgtata
    87721 taaccattac tcatacctgg tttttttaat tgtacggctg atatattttt aaaggggata
    87781 gttttttctc ctttaatacc ttgattaact aaatttataa atccttttct aataattgaa
    87841 atagaatcat cagttacttt aatcaaggtt tttcctggag attttattaa aatctctttt
    87901 tccatttttt cttcctcatt tctgtgatat gatttttttt gtagaagctc atatatgagg
    87961 aaagagcccg tgttgcagca cgggcttttt taatgttttg ctgaaatcgg tttttttaat
    88021 aattcttgat gtattcatac atattgcctt gcgtaagaat attttttctt aaaatagcat
    88081 tggcagacag cataacaagt gtatctgagc ttattctgaa tagaataata ctccataaat
    88141 ttttcgagat taaactgaga ttcatcagtc agttcgttct caatatagat atttaataaa
    88201 attagaatag ctattttatc tgcttctgtt tcaaattttg agtgaaaagt tgtagaagta
    88261 tcgtacaaaa ctgaaaattc aaaatgagag gcgctgaaat gtgcaagttc atgagataaa
    88321 tgaaaggctt ctgcagtttc accgtataga ttttcattca aaaaaatgat tctgggtttt
    88381 ggatagtaga aaccaggctc tttcatctcc atataaacta ctttcaaatt gtattcgctc
    88441 aacatttctt tcaatttcaa atacatacaa accatcactc caactattca ttttcctcta
    88501 aagctttagc aattgcaatc gctttacgca ttgtctcctt agatatttct tttccatcaa
    88561 aagaaaaaac agtatcgtct tctgataaat ccacatgttt aggggtttct ctttcttctc
    88621 tacctagaag gtagtctaca gagacatcga aatagtcagc aatccgactt aattcatctg
    88681 aattaggagt attatttttc cactttgcta gataaccgtt agaatatcca aaattaattt
    88741 ctaactgccg tatagatatc tttttcctct tcgccaagtc ttttattatt tcataggtat
    88801 tcatcgataa aacaaccttt ctaaatgttt acaaaaaaag tttagaaaaa tacgcagaaa
    88861 taatttgact aattctgagt aatgagctat actatatctt gtaaacagat ttaacaacta
    88921 aaaagacaac aaaaaacact attgatatgt aaatgcagac cgccaagaaa gctttaaaat
    88981 caatgttttt atgtcttatt taattatgct cttagtatag agtattactc agatactgtc
    89041 aattgaattt agaaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggaatgagaa
    89101 atatgaacac accgcaaatt tttaatttcg aacaaaacga agttcggaca attttagtaa
    89161 atgatgaacc atattttgta ggcaaagacg ttgcgagtgt tttgggttat tcaaacacta
    89221 aagatgcttt gtcgcggcat gtagatttgg aagataagat ggggtcgcga atcacgacct
    89281 caggtcaatc aagagagatg acaatcatca acgaatctgg tttgtacagt ttaatcttaa
    89341 aatcaaaact tccctctgcc aaaaaattta aacgttgggt aacaagtgaa gtgttgccag
    89401 caattagaaa acatggaggt tatctaactc cagaaaaagt agaagaagct ttgcttaatc
    89461 cagatacaat cattcaatta gcaactcaac taaaagaaga aagaactgga agattaatcg
    89521 cagaacaaaa gattgcagag tacgaaccta aaatctccta tttagatagc atattatctt
    89581 ctacagattc agtaacaatt agtcagattg cagcagatta tgggatgtct ccacaacaga
    89641 tgaataaatt acttcataaa ctaggtattc agaaaaaagt cggtaaccaa tggttattgt
    89701 gcaaaaaaca catgaaccaa ggatacacaa aatctcatac aactgagatc ccgaaagccg
    89761 atggtggcac taaaattgta atgaatacca aatggacaca gaaagggcgt ctatttatct
    89821 acgaattact aaaaaaagaa ggatattacc ctcaaatgga tttagaggaa attggttaga
    89881 aaggagtttt agtatgactg acattgcaga aatcactcaa cgagatagag aaaaaatcaa
    89941 agaatatgtc gaaagttcaa agttcttaac ttacaccatg cttgctgaaa gatttggaat
    90001 tagcaaaagc tacttatctt taattttaaa cggtaaaaag acttctgcag aagcaaacag
    90061 aattatagat tcgattatca ctatgtacga attgtaaagg gaggaacagc taatgcaata
    90121 tctagaagca aaaatcccaa ttccagaagg ctatgtaatt atctcccaag tggattatga
    90181 ggagttaaaa aaagctgatg atactggtag atggatgacg ttgccagaag tgctagaacg
    90241 gattaacaga aaatatgatt ggtttacttc tagagtttta aagaacccaa gatatagaaa
    90301 tattatcgat atcgagaaaa acaaaaatgg atttgtctat tatccagttg aaggaagaga
    90361 cacatatcta tttttaagaa gtaaaacact tgaattttta gaaacaaatt tttcggaaat
    90421 cttaaggagg caagcggatg ggaaaattta atagagcgct agtgttcagc gcaccgctaa
    90481 tcatctacgc tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca
    90541 tggtttggat ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa
    90601 ccgagggggc tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact
    90661 cagtttaatt ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt
    90721 ttgtggattt gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta
    90781 ttaaaattaa ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga
    90841 atatttagcg gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca
    90901 attaatttct taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct
    90961 aaattaacta acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc
    91021 atatctgcag cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt
    91081 atttttatcc ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa
    91141 attgttaagt gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa
    91201 aaggagagaa gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac
    91261 gagcttacaa gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga
    91321 tgcactaaaa aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga
    91381 ttcatatggc agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc
    91441 tatggggttt acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa
    91501 cgaaatggaa gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat
    91561 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
    91621 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg
    91681 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
    91741 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
    91801 gagattcgat gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat
    91861 ggaaattgat caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg
    91921 aagcgctaag aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca
    91981 tcctaagcca acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc
    92041 gccggcaagc aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa
    92101 agaactttcc actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga
    92161 ggaaacaagt ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga
    92221 tcaagatgat ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc
    92281 gaagcattcg aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct
    92341 agaagagtac gacagagata acttgattga tatctcttat tacggaccag gtaagaaata
    92401 cattgctgaa atggaggcag aatttgatga gtgaaatcaa agggacaacg aactttgaaa
    92461 aactttttag tcgtaagtta aataaaattc tcaagaaaaa aggaaatttt gattatttat
    92521 cttgggctca cgcgtgggag attatgaaaa agaatgatcc acaggcaacg gtaactatta
    92581 atgagtacaa acactacagg gttgtttctg gaactcatca agactttctt gttgaggaat
    92641 ataaaccttt tcttatggat gaaactggga cttatgtatc tgtctcagta acggttaaag
    92701 gacacacgga aaccgaatta tttcctgttt tagattatcg aaaccaacca gttgttaaac
    92761 caaatgcaat gcaaatcaat aactcattga agcgatgctt tgtgaaagca ttggctctac
    92821 acggactggg attatatgta tttcaagggg aagatattcc aacaccacct agaatcgata
    92881 caaagaaatt aaacatgcta gagacgattc tagaagcttt caatgagcag atgggtaaag
    92941 atatgaccaa aaccttaatc gaatatgtta atgagcagac agataaatta gggctcttag
    93001 ctgataacgt tgaaactatt gaacagttaa gctatgagca atgtgccttg atggagcgag
    93061 caatagcagc taagagaaaa gaattagata agaagtgata tgagtgttta aaccattaat
    93121 cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa gacataagcg caaccatcaa
    93181 tgaggaagtg aacattgatc gactaaagac gatgtatgac ggctacgatg gtgatcgagt
    93241 cattgaaatt cgttttattg atcctagacg tttcaccgta cagcaacgaa acttcatcta
    93301 tgcgctgata ggcgatattt ttatcgatac aggcatgcca acggacttct ggaaggaatt
    93361 cttctacttt cgttttgaag gtgtcacagg gcgcaaaata agcctgaaag acgaatcgaa
    93421 tacaactgtg agtgatgcca atgtcttagc aaatatcatc ttagatttca tctttgaaca
    93481 tcatattcct ttcaaagaag gctatgagat tttacctgcg aatcaagaat attacttcta
    93541 caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa acaggagctg acatcgatca
    93601 ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt gatcatgcag agtacacatt
    93661 tgcagcactc tgcagaatcc atcacacaga gaagcacaaa ataggtgtga ttaatttcaa
    93721 aaataaatat caaatcaaag ggatcaaatt aaaccagaaa acaatcaaaa agttaaatat
    93781 tggaggggaa aaatgacaga acatcgaagt tattatgcga ttataccagc caacgtaagg
    93841 tacgacaaaa gacttaaacc aaatactaag ttgttatacg gagagataac ggccttgtgt
    93901 aatgaaagag gcttttgttg ggcaggcaat gagtactttg cagatttata tggtgtgaat
    93961 aaagagacca tatcgcgatg ggtaagtgat ttgattaagt ttggatactt gaatcgggaa
    94021 atcatttaca aagagggtac caatcaaata atcaataggt acctacgaat taatcaatac
    94081 cctattgacg aaaaacgcaa tacccctatt gacgaaaaag tcaaagataa taatacatct
    94141 attaataata catttaataa tacaaaagaa tatataagag agttaccgcc ttcgaaaaaa
    94201 tcgaaggcta agcccgtccg tcataaatac ggagagtata aaaatgttct tttgtcagat
    94261 gagcagatgg agaaactcaa aacagaattc cctaatgatt accaagagcg aatcgaacga
    94321 ctgtcagagt attgtgaatc atctggtaag acttataaaa actatttggc aactattcga
    94381 agttgggcaa ggaaagaaaa aagtgaacct aagaacgcaa gcagtggata caagcgcaca
    94441 ggaagacgag agaagcttcc tgaatgggca atcgaccaag aagcctatct caagaaaaaa
    94501 gcgctagaac gagctaatag acaatcaaaa gcaccattct aagaggtgga aaaatgaaga
    94561 tcgattatct agaactaatt aatgaaatag cgaattataa aaagggcgag gaattagacg
    94621 tcctgagaga cgtatatgat caactcgaag aagctggaat tgaaggaatt aagaatgatc
    94681 gttcgagttg gagtaaactc agatactatt tcgcactcta tatcgatgga acgcaattaa
    94741 gaaatttagc atataccaaa ttactattca ttgattgtgt taaaggcttg caaaaacatc
    94801 ttaatgaact tgagcaggtg taaacaagat ggacctaaag acatttacag cacagatcga
    94861 attaatgcat caagaagctt taagacaaag tgtgtcgtac gaagacaagt ggctcaacac
    94921 atttcatggc ggacgtgaga gcgcacttga tcaagtactc aaattactga aaggagaatg
    94981 tcgggatgga taagaaagcg gcaatgcagc gaattatcga attgacttat tcagaagatt
    95041 ggcaggaaga taaaaaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg
    95101 aaaagcccaa acggagaacg ccgagaaaga ttgcaatctg gcatggtgat cgaattctag
    95161 taacgggtac cgctaaacag ttatctgaaa ttactggtct gagcaaaaat attatttggg
    95221 atagagctag gagcttatgg attgattcaa aaggaagaca gtttaggtat gtggaggaga
    95281 aataatggac gaactaatcg cgaaagtaga gcagtgggca aaagataagg gattggatca
    95341 agcggatttc agcaagcaaa tgttaaaaac ggtcgaagaa atcggggaag ttgccgcttc
    95401 tctagctaga aaagatgaac atggtttaag agacggaatt ggcgatgtag tagtaacctt
    95461 gatcatcttg gcaatgcaaa atgatatgga tttatacgag tgcttggatt ttgcttatga
    95521 cgaaatcaaa ggtcggactg gaaagatggt caatggtgtt ttcgttaagt ccagtgacct
    95581 gaaataattc cattaatgga agaaataact gaataggagg atagacatga ttgatgcaaa
    95641 acaagtgcaa aaacagaaag atggaatgtt gatgtttgaa gcatatgttc taccatttct
    95701 taatcaattt gaagttttgg aatgctctgc cagcggagaa gaattggagt atgttgtaat
    95761 tcgtgaaacc aaagaaaacg ttcagaagct aaacgaattt ctttgcacta ttaactgttg
    95821 ggatatgata gctccaggat ttttatgtcc agctatggga gaatttttgg aatattgtcg
    95881 tcttgaagat gcaggcactt tggatttagc ttatctggta tacaactacc taaacatcaa
    95941 cacagatcat ttgtggtttg gcactgccga aagaaaatgg gtagtccgct aaccaccaat
    96001 atcaatgaaa aaaggcggtt tcctgaaagt ggaattgccg ccattgctgt aaggttcttt
    96061 acgcgactca aggtcacaga aatataaatg caaatctctt ttgaagtgat ccaaagctct
    96121 cggaaaatgg aagttttttg tttcaaagaa taaatgagat ttgcgtcaag aacctgagag
    96181 ttatttccaa aaaagcttcc aaagaaccga atgagctaag gacatcatgt gtaaacacat
    96241 aatatagctc tcctttcacg gctaatcata acatgaaaaa tattcgatgt cagtaggaaa
    96301 aaaggtgaat tatgaaaaga atattaaatt atcccggcag taacagattc tgcaattaaa
    96361 ctccgaagct gatgtatcag tatcaactgt agaatattat gagcgtggag aaagtcaagc
    96421 agtcacaaat gtaaaaatcg ataacgtcaa cggtcttatc tgtttagatg aaggcgatag
    96481 tatgaatgaa attgctgcag accatgaatt atcagaaact gctttaattg atcaaatcga
    96541 attagctaga caatactttg ttccatatgc agatacctgg tccaagcata agaatgacat
    96601 catttttcca gaggaacaat gagagcaact gatccagtaa ttatccttga ggaagccaaa
    96661 tttatttgga ctcacgaaga gatagagcaa gcacgcttgc tcttttctca aggagttaag
    96721 ccgagcaaag tagctgaaat aatgggtcaa aagattcttg atgtcggatt gcttttgctc
    96781 catctagcag aaaaaatttg atttgaggtg aaaatgatga ttcaacttgc aggcatccaa
    96841 acagggaaaa tttatttttc tggagaaagc aaaagcgaag ccaaccaatg gctattgaaa
    96901 acttacacga acaataagaa gctaaggaaa aaatatcctg atttgttgct aaaggatgat
    96961 cagattatgc cggaaccgat gattttgacc cgtaaggagg gaagttgaaa ctaaaaaacc
    97021 agccgtgtgg gctggagttg ctatttcttt ttaataatga gtttttctaa atttaattca
    97081 ttctcacgag tttgcgtcca ataatcagaa taatatttta attgtttaag agtaggaatg
    97141 tcgtcttcgg taaaattaga aaataattct ggagaattat ctttttttaa ttgttctaat
    97201 cctgattctt taaaacgttg ttcaacgagt tttactaaat tgttgatatt catgataaat
    97261 caccttttta aatttaattt cgatttgatt aatcgataca agtattatat caaataaaaa
    97321 agctgattta gggggtctta caaaaacact aaaatttttt caaaaagagg aggaacggtg
    97381 gtgaaagtca gagaacaatt caagatagtg agcgacgaag gtaatgttta caaaataaag
    97441 atcgttaata ttaatgaata tcgagaagca gatcacaaat atttggttga cttatacatg
    97501 aacgataaat atttgggaga aggattttgt ggagattcac tttttgatga tgagcgaatt
    97561 ttgaaggagg aaacagaatg atccacgaac taaaaatact accagaatat tttgaagcag
    97621 tgacgagcgg acgcaaacaa ttcgagatcc gcaagaatga tcgtgacttc aaagttggtg
    97681 atcagttaat actacgtgaa tataaaaaat acattaaaca tgaattacct ggactaacga
    97741 gaccaacatt tagttacact ggtgactcat acaaagcaga gatcacttat attactgatt
    97801 acatgcagaa gtccggatat gtagtgttgg ggattaaagg ggtgaattaa atgaaagtat
    97861 acatcttagc tatcacagag ggcacatgga tgttcccagt cggttccgga aaaatctata
    97921 aatcaaaaac agctgcttat aaagcatttg aaaaatacaa aaaagaaaac ggtggcggca
    97981 ctaacgcaaa gattctagtg gctgataact ggcatgaaga aggtgaacgt aattgattta
    98041 cctgttcatt ttcgtggtac taactatttt tatgctagta atcttattca aaaaaagatg
    98101 agtaaagacg aacgaaaaat tctggccaac ttttatttag tattaaaaat cgaacaaaat
    98161 gtgttttcgg aaaggagaat tgtatgggaa aaacaaaatc aaagattaag aagaaaaaac
    98221 gacgtttgga acaaaaggca atccagaatg gaacggctaa gaaaaaataa aaaagctacc
    98281 cctttcgaga tagcagcgca atactattct accataaggg gtggcgtttg tgagatttca
    98341 ttggttaaaa gattaccaag aactagatga gcagattctt tacttaaagt ggaatcttaa
    98401 taaaagtaag cttgaattga atcgatgggt ctgtggtgat ttagcaaacg tccgcatcga
    98461 aaagaattcg agaacttctt ctttagaaga gaatatccaa aagatagaaa atgaattgga
    98521 attgttgatc gagcaaaaag aagaaatgtt attgctgata gatagctttt ctggtatcga
    98581 taatcaaatt gttaagatga aatatgttga tcaaatgagc ttagaagata ttgctgaatc
    98641 tgttgggtat agctcatcgt atgtcagaca gcgacatgca gaaattagaa agacattgaa
    98701 ctttttagat gagtatgaac acagacaagc tgaccgattg aaaaaagaaa acgaaattga
    98761 tttttacaat agcgagaagt acaaagaaca actctcttta ttctaaaatt acactgttca
    98821 ctaaatgttc ggtcattgta gctatgtaaa cattgttagc agcatgatat tctattagtg
    98881 tcaaaaaaat atgaaagagc caagatatcc caaccgtttt attaattggt atctgtggct
    98941 cttttattct tttaattagt agttggcata aagttatttc catttataaa ttgatcagct
    99001 aaatcagcat cataatcagc cgcgataaat tgcgctgctg atttaataaa tgcatccata
    99061 tcatgaacat ctttattagt atgtctacga acatagtgcg tttcatcatt accaatccaa
    99121 gtagcagctt tggctaatct ttggagttta ggaaaatcat tcaaataatt ttcaattgtt
    99181 ttacctaata acattttttt tattttatct tcatcctctg aatttttaga tattgcataa
    99241 tctttaatta gaaattctag agcttttcta taaccaactc cagcaatttg ttccaattgc
    99301 tcagatttag cttgtgtggc ttgagtataa atatcaacaa atgtaggaga aactttttca
    99361 atattttctg gtaaatctat tttaatcggc ggacggtaat aatctttaat taaaatattt
    99421 tcagaatatc cttcatcttc attaaacgta tttataaatt gtaaactata aaatttgttg
    99481 cactcatcat ttatacactg tactaatacg ccaaagattc tatcattatc gaacgcacta
    99541 tatgatgaaa ccccaaaata cacatacgga gtcattatat ttccgcaatg cggacaacta
    99601 tttggcaaat aaattgtctc tgtattttga cgatcacgat acttaatatt aacttcttga
    99661 agcattaaaa cacctcgcat cttttttata aatcatacca caaaatatct gataggagag
    99721 taaacaaatg aatgattttc atgaagcggt acttagtatt gaagtagaat caagtttggc
    99781 taaagtttat aagaaagcaa ttgaagcaga gaatagtccg tacagagaaa attggaatgg
    99841 taatcatgca catgtgcaag tggagaatga tgattacaga actggaatga atacgttagt
    99901 tatttcttta ttgtcacaca ctctacccaa tctgcaggaa acgattgaat ggtatgagcg
    99961 aatgggcgca aaagtaatcc gtacaaatta caaaggagag aattaaaatg gcaatgatca
   100021 aaattagaac atccattagt ggaacagagt attgggattc agaaaagaaa aggacagtag
   100081 ttgttccgaa agatcaagaa ccaaattttg aagtaaaaga agaaaaagga attttgagtg
   100141 atggcaaatc atttgttgct gtcaatggcg aactcatagc tcatagcagt gaaattcttg
   100201 atagcgatgg aaacactgct gctaattttg atggagacga agctactaat gatcagtcca
   100261 ctgaagatac ggatgaactg gacaatatga ctgcaaaaga attgcgtgca tatgctaaga
   100321 aacatggtat tgatattcct ggtgctatcc gtgcaaaagg cgatatcata aatcttattc
   100381 gtgaagtaga atgaaatact gtcagtttga cggatgcacg aacaaaatag caaagggtat
   100441 ctactgtact gaacacaaga gatcaagcag atcacgcaag aagaagcagc aagcaaagtc
   100501 tgtttatcat catgagaaca aaccattcta tcgaacgcaa gcatggaaag atatgcgtca
   100561 atttatttat gaaagagaag gtggtcattg tcagcggtgt ggtcagttca tatttggaaa
   100621 gagagcacac gtccatcaca ttgtaccaat caaagacaac gaactgctta agcttgatcc
   100681 aaacaatctc atgctattat gttcaaaatg tcatccaatt gttgaaaacg aaacggaaga
   100741 caaaaaagtt tttccttcgt atttcaattg aagcccccct attcattttc aaaatttttt
   100801 cgcgtgggga gatagggtag cggggagtca cgcgcatcgt taggtcaaat ttttcaaaaa
   100861 acaaaggggg gtgtatacaa aaatgacgac taaagcgcaa cgtaaagcga ttattgatga
   100921 aaaagtaagt gctgaaaaag ctcgtatctt agaaataatg aatttgtctg atttgtacac
   100981 catcactctt gatccattaa tcgaatcata cttggatatt tttgaaattt accaacacaa
   101041 atatttattg tggaaggaaa aaggatttcc ggagacgcaa aaattcacga ataaatcagg
   101101 agctactaat caatcaaaac atccattggc acaacaagta gaaacttggg cagataagaa
   101161 gatgaaagct ctagatttat taggattaac aaacaaagca aaaactggta gactaattgc
   101221 tggaggatcg accgcaagaa aagatgaaga aattacacgc ccagaagtaa agccagtaga
   101281 tgaactagca gcgcacagaa ataaatggcg taagaaggca ggtgctgaaa aatgattgaa
   101341 cctggtgtaa attatgctga tttatttgca aaagaagtaa gaaagaaacc taagaagtat
   101401 cctaagaccg ttcgtttagc aatagatcgc tggtatcagt ggaaaaaaag aaaagatatt
   101461 tggtttgatg tagatcgcgc gaatgaaatg atggattggg tagaatcatt tattgttcac
   101521 acaaaaggag aaatggtggg aaaaccgttt cttttagaac cctgggaaaa atttatttat
   101581 tcttggattt atggttgggt taaagaaaat gaaaaaggcc aaattgtacg cgttactcgt
   101641 gaggcttatg tccagattcc aaaaaagaat ggtaaaacat tgattgctgt cggttcatta
   101701 ggatatgcaa tgtacggaga aggggcgtta tcagttgatt gctatgcttg cgcatctgat
   101761 tttgctcaag cgcaatatgc tgccaaacct tttgcagtta caatcttaaa caatccaatc
   101821 ctacttgaag gaactaaaat atttaggggg ccaaagggaa ctgtttctag tattacttat
   101881 gactatattc atgaagatat ggcttactca aataaattta ttgttcaaac caagaatatt
   101941 gataatatcg agggatccaa tccatatttt gtattgaacg atgaattgca taaacaggag
   102001 aaaatggaac agtatgacaa ttttaagtct gctcagatat ctttaccaca gccattgatg
   102061 tttaatatct caactgctgg gaaaggttcg tcgtctgttg gaatgcgtgt atatcgcgaa
   102121 gcaaaagaag tgttgaaacg tgatgataat gattcaaact ttgttctaat ctatgagcca
   102181 aataaaaatt atgattggac ggatagaaaa gtctgggaaa tgtgcaatcc taactgggga
   102241 atatcagtcg atttatctgc tttagaatcg gcatttaaaa cagcgcaacg gtcagctcat
   102301 tccaaagctg aatttctaac gaaacacttg gatgtatttg tgaacggcgc ggataatttc
   102361 tttgaacagg atcaagtaga gccatgttta gttaccacac aagaacttgg tgatttaagt
   102421 ggcgaaccat gttatatcgg tttagattta tcacgtacac gagatttgac ctgtgtatct
   102481 ttaaacttcc caacatggga tgaagatggt aaagcggttc ttaaagtaaa gcaactttat
   102541 tttattccga atgaagattt agagtttcga gaaaaagaag ataatgttcc ttacagtgat
   102601 ttggctgaac aaggtttcgt tgaattttgc gacggaaaaa tgattgatca agatcagatt
   102661 ttgcagtaca tagaggactg catgaattta tatgatattc agcaagtgaa ttatgatcca
   102721 gcgatgagtg acaaacttgt tgagaaatta gaaaacttag gattggaatg cgttgaagtt
   102781 gctcaatatc ctaaagtatt gaatgctcct tttgacgatg tcgaacggtt gttttatgag
   102841 aaacgaattc aatttgataa tccattattc ctttattgca ctttgaacgt tgtagcaatt
   102901 actaacatca atggacaaaa agcgccaagt aaacgtcaat caaagaaaaa gattgatggg
   102961 ttcgtggcgt ttttgtgtgg tcataaggaa acgatgaatc aaatgacaga tattgattca
   103021 gatgagttag atgattacct aagttccatc tatagataaa tagaaaggcg gtgaaaaaaa
   103081 ttgaaattac gtgatcggtt atcgaatgca gtctattctt ttatggaaaa acgtggatat
   103141 atcgaggata tttttggaca ctacacacgt tatggtcaaa gatatgtgac ggattcatct
   103201 atcatggaat cttctgatgt ttatgaatta gttcaagaca tatctaatca ggtggcattg
   103261 gccacaccaa ttgttattgg tcccgacggc aacgaagtca aagatcatca cttactaaat
   103321 attttgaaga gtcccaatga ttatttgaca ggattcgaat ttaccaaatt ggaaacaaat
   103381 actttattga tcaatggtga gacattccca ttaacggata gggatcagct tcatttggcg
   103441 tacggtgtaa caactaaaat caatgaacga cttcaagaag aatttgaaat gaatggtcaa
   103501 aaaatacctg gtcaaatgat tcgacacatt aagaacatcg gaactgattc attaaaaggt
   103561 gctggaataa ttgatcttgc aagaaacact ctagaaggcg ttctgagcgc tgaaaaagtt
   103621 ttgacggaca aatatactaa aggcggttta cttgcgttca tgcttaaact agacgcccac
   103681 atcaatccaa ataatagcgc ccaaacaaaa attgtcaaag ctatattgga tcaactggaa
   103741 ggaacgcaaa atgagagtga tcattctgtt aagatgattc ctttgggaaa aggatattcc
   103801 atcgagacat taaaaagtcc tgttgatgac gcggcaattt tgaattattt aggtgtttac
   103861 aaaaaagact taggaaaatt tctaggaatc aatgttgata cgtatcaatc gctgatgaag
   103921 acagatattg aaaaagcgat gatgtatctg cacaacaaag caatcaaacc aatattgaaa
   103981 aacaagagcg aacattacac cgctcttttt tttatgccta attctggcta tagagtggaa
   104041 tggaaaatta atattttgga ttttgttcct tactcaacaa aaacaaatat tggctacaac
   104101 atcgttcgta ccgggattac aagtccagat aacgtggcag aaatgcttgg ttttcctaaa
   104161 cagaatactc cagaaacaca agctatctat atttcaaatg acttatctag gattggccag
   104221 aaaaatgcaa cagatgattc cttaccaacg aatgatcaag acttgaaagg aggtgatgga
   104281 aatgaagaag aaggaaattc gcacgattga catcaccaat ctttcaacac gttccgatga
   104341 agaaactcat acgaggacca ttagcggcta cgctgctgta ttcaacagtc aaacactttt
   104401 atgggatgac ttaagcgaag tgattgttcc tggtgcattc tcaaaggcaa ttagtaattc
   104461 ggatgttcgt tgtttgttca atcacgattg gtccaatgtg ctaggacgta ctaaaagcgg
   104521 aacccttcgc cttgaagaag atgaacgtgg tttgaaattc gaagttgatt taccggacac
   104581 aacggtagca agggacttag ttaaatctat ggaacgagga gacatcaacc aatgtagttt
   104641 tggttttgtg ccgactgaag aaacgtggga ctacaattct gaaccaatgc ttcgaacaat
   104701 ccatgaagtg gaattatacg aggtttctat tgtgcctttg ccagcatacg aagatacaga
   104761 agctgcatta agaagtcgtg atgaattaga aaaaaacgtc gaagaaagaa aaaaattaat
   104821 caaaaaaatt aatcaagcgc tagaagcgta ggaggaaaaa tacatggata aagaattatt
   104881 gaaaaaaatg aaggcacgtc gtgagcaacg attgactgaa ttacgtgaaa aagttgaatc
   104941 aggagaatta cgcgaagcag atttagaagc cgtgaaagaa gaaattgaca gtgttattga
   105001 tgaattgaac ggaattaaag acgaattagg cgcagattct ggaactgatg aaacagacga
   105061 caatacagat gatcaatcga atagtacgga ttcagacgaa agccgttctg gtgaagacaa
   105121 cgatcaagaa gaagattcag acagtgaaga tagttcagaa aatcgttctg gaatgattac
   105181 tcaacagcaa cgagatggat tacttggatc aattaagaac ggattggagg cacgtgcaaa
   105241 aatgaccaag aaacaaaaag atcaacaact acgaaaagca tttgctaatt ttgtagctgg
   105301 aaatatttct gaagcagaag ctcgagcttt agggattgaa gctggcaacg gttcagttac
   105361 tgtcccagaa gtaattgcat ctgaagttat tacttatgct caagaagaaa atttacttcg
   105421 taaatacgga acagtggtgc gaacatcagg agatgtcaaa tatccaattc ttgtgaagaa
   105481 agcagatgcg aatgtaaaca agaaagagcg ttcaactgat attgctgaaa cagctattca
   105541 gtttgatgaa attttgctta atcctgccga attcgatgct ttggcaacag taactaaaaa
   105601 attattaaaa atgtctggtg ttccagttga agatattgtt gtggaagaat tgaaaaaagc
   105661 ttatgtgcgt aaagaaatca attatatgtt caatggtgat gacgctggaa atgaaaatcc
   105721 tggtgcatta gccaaaaagg ctgtagcatt taaaaaacct ttagatctaa ctgcttcagg
   105781 tgctgggcaa aaattatatg atgcattaat cgaatttaaa aatacaccag tgacagaagt
   105841 gatgaaaaag ggacgcttta ttattaatcg agctgctttg actgccattg aaaaaatgaa
   105901 aacagatgat ggatttcctt tgttgcggcc attcacacaa gcagaaggtg gaattggtta
   105961 ccaattagtt ggctatcctg tggattggac agatgcagca gataaaaagg gtgaaccaga
   106021 cacgccagtt ttatattttg gcgatttttc tgcatttaaa attcaagaag ttattggtgc
   106081 cttggaaatt caaaaacttg ttgaaaaatt ctctggtaaa aatcaaattg gattccaaat
   106141 ttacaacttg ctagatggtc aattggttta ttctccattt gaaccggcag tatatcgcta
   106201 cgaaattaca aaaccagttg gtggttaaga tggaagagca aactaaagaa ttgtctttag
   106261 aggaaaaatt caaatcacat attcattttg aagagggcat ggatgattct ttgctctctt
   106321 tttatttaaa tatggcaaaa gattatgtca aaacagcaac tggtggccaa caagaatatc
   106381 ttattttgat ggttgccggc attgcctatg aatatagagt ttcagaggat gaactcgaca
   106441 aagctatgaa tgccatgacg ccatttatcg tgcaaggagc gattcaaaat gccgaagaga
   106501 cagactaata atctcagatg gaaagccgaa ttgctagaca tcaaaacagg aacagacgga
   106561 aacgatcgtc caactacaat ttacgaattt aagcgtctaa tattctatga agaactcggt
   106621 gtgacttctc aagaaaaata tttatcacag caagccaaga cagacgttgt cagacgaatt
   106681 aaagtcagat gggataaatc catcacagag aaattaagtg cgctcaaaat tgattctgta
   106741 acgtataaca ttactcgcat ttatacgaat cccgatgcaa gagaaatgga gttgagttta
   106801 gcttatgtcg attagctttg atgaattgaa aacagcgctg aaatcaacaa agttagcagt
   106861 gttcagagac aaagccagat tagggacgat gtatccatac attgtgtact caaatgtgag
   106921 taacagcaaa aaaatggcat ccggtaaagt ctataaaaaa ttaccttatt accaagtctc
   106981 tttttttaca cttggaacag aacaggattt agttgttatt gaacaagcgc tgcaaaatgc
   107041 tggcattcca tattcagatt ttacaggtat acaaggtgat gagaacgacg ataccgtaac
   107101 caactattac acatatgtga ggtgtgtgga aaatgccaag taataagaat ggtttttctg
   107161 aaatatcgga ttatttagga aacctttcaa aagttgatcc gaaaaaatta tcgttggaat
   107221 ctttagaaga agcagctaac ttttatctaa agcagttgct tcctaatatt cctaagtcgc
   107281 tcttaaaaaa gaaacatatg cgcgatcaaa taaaagtagt agttgaaaac gatcaagtga
   107341 aagttcaatt tgaagagacg gccttttact ggcgctttgc cgaaaatggc acaacaaatc
   107401 aacgtgccca acattttgca agtggtacgt atgaacaaaa caaagaaaaa attgaagaaa
   107461 ttatgactag aaaaatactt gatttatggg aagggtgatt ttaattggga aaacaagata
   107521 ctttttattt tgaaggattg gacgacattt taattgcgat gatggcaacg ccagattctg
   107581 ttggaactgc accgacttat agtgaagttt tacgattgcc aattgccaca aaaatcggtg
   107641 tcaaagggaa tggaacagca ttagaaaaat gggcatccag caaaatgttt cgtcgtgtgt
   107701 cgcgagaaac aaaacatgaa cttgcgttgg atcacgtggg tattccgata gaagtgatgg
   107761 atgaaatcaa aggattagtt gctaaaagcg gagtaacgtt tagcaaaaat accgcacgag
   107821 aatttccgta cttcgccttt ggatttattg gaaatattga aaatggcggt aaaaaagcag
   107881 tttggtatcc taatacacaa ctatccaatg tgattgacga agagtatgcc actgcagagg
   107941 aagaaacaaa aattgacgat gtaactgcaa atctggtttc catcggactg aaatacaata
   108001 atgtgatgta tgcaagtttt gattcaaata gagacggagc ttctctagaa ttatttaata
   108061 aattcattgc tcaacctgta tacgatgaag aacagtggaa aacattggct aaagttggag
   108121 gtgcgggtta atggctcggt tatctgatta tggaattcac gttgaagact taaaaaattc
   108181 tgctgttgta attattcaag gcgcagaatt ccctatttca tttactatgc aaacgatgga
   108241 atttatagca gatgtgtatg gtggagatta ttcgcaattt gaatctgata tgaacgccat
   108301 gctatctaaa aaagaaggac aaatttcttc tgctaactta tcgcctagtg atctaaaaat
   108361 catgcgtgcc ttgatttatg ccatgctgcg tactggtggt ttagaggaag atccagaaac
   108421 tattttcaaa tttttgggaa tgagtggaga ggtgttgtct gcttatagta cctgtatgga
   108481 aatttttgct agccagacat ttcaggtgga agacctaaaa aaatccaaga agccacaaga
   108541 ctttcaaaaa gcgcaagcaa aaagaaagaa aaacaaaaag aatcggaaga gataggaact
   108601 ccttggagtt tttatattta cgttgctctc actctattga attggagtga gagtttcttt
   108661 ttaaagtcta cacctaactt gtggctcaaa tcttatttac agtggttaca acaaaacacc
   108721 gattttgaac cacctcaatc tgtaactatg gataaatcgc cttggtggta gaaaggagcg
   108781 ctaagataaa tggctggtaa agaatctgat gtcgttctta attttaaaac gaatggcgaa
   108841 gtcagttatt cgaaaacaat caaagaaatc aacaaagaaa tgaacttagc cgctgccgag
   108901 tacaaaaacc aagtgtctgc gatggacaag gatgcaactc aaacagaaaa attgcgagcg
   108961 gctaaacaaa aattagaaaa acaattagga ttggctgaac aacgatctca aatgctgaga
   109021 gaggaatatg aaaaatctgt caaagaaacc ggtgaatatt ctgctgaatc agaaaaactt
   109081 tataaacaat tactcaattc tgaaaccgga gaaaataaac ttcgtacagc attagaacag
   109141 acgaacgatg cacttaaaga acaaggtgac gtttctgttg atacagcaaa aaaactccag
   109201 aaaatcgaag aaacaggtga gaaagtaaaa ggcgttggtg aaaaaatgtc tgttggagta
   109261 accgcgccta ttgtagcggc aggagcagca ggacttgcag catttggtga agttgacgag
   109321 gcacttgata ccatcattac aaaaaccgga gcaacaggtg atcaagctga tagactttca
   109381 cagtctttca aaaacgttgg ttcaaatact catttacctt tacaaacggt tggggaagct
   109441 attggtgagg taaatacaca atttggattc atggataaaa aactggaaga ttcaaccaat
   109501 tatctcctac agtacgctga aatcaatgat acagatgttt cgcaatcagc aatatctgct
   109561 cgacaagcta ttgatgctta tggactagaa tatgatgatt tgaattctgt ccttgatgta
   109621 acaacgaaaa catcgcagaa tactggtcaa tctgtagacg acttgatgca aaaagcaatt
   109681 gatggcgcac cacaaattaa acaattaggg ttgagctttg gtgaaggggt cactttactt
   109741 ggacaatttg agcaatctgg tgttgattca agcgcagctt taagtagttt atctaaagca
   109801 acagtggctt atgcgaaaga cggaaaatct ttaagtcaag gactagaaga actacaagac
   109861 aaggtaaaaa atgcaggttc tgaaacagaa gccatcaatg cagcagcaga agtattcggg
   109921 actaaaggcg gtcctagaat ggccgatgca attcgtagag gtactttgaa cctggaagat
   109981 ttagcaaaaa ctgctggaga aagtggggga gctgtaggag acacattcga tgctactctc
   110041 gacccaattg atcaagccga tcaagctatg aacaatgcaa aattagcaat ggctgacgta
   110101 ggtgaagcag tccaagtaag ccttttgcct ttttttgaaa aagcaacttc cttattacag
   110161 gaattctcta aatggtgggg gtctctagat caagatacaa agaattggat cataacaatt
   110221 gctggtatag cggcagcagt tggaccagta ctaatcgttc ttggttcact tatgggatct
   110281 gttacaaaaa tcgttgggga agttaaaagt tttataggag tttggcaagg acttgctggt
   110341 ttatttggaa tgtcgggagg ctggtttgca ttagcagtta tagctattgg cgcattagtt
   110401 actggactaa tttgggcata taataacgtt aaatggtttc atgatggagt gaattctttc
   110461 ttcaaaggtg tttcagatgt agctgttgaa atatttaatt ttttaagtgg ttattttggt
   110521 ggatttttcg atggtgttat agcaaatttc aataattttt ataatgctgg aaaacgaatt
   110581 ttcagtggtt ttctagattt tatcactgga gtatttacag ggaattggtc aaaagcatgg
   110641 caaggtctag ttgatatttt tggaggtatt ttcgatggta ttgttgctgt agggaaagct
   110701 cctattaatg ccatgattgg attgatcaat ggatttattg gtggattgaa taatataaag
   110761 ataccaaaat gggttcctgg tgtaggtgga aaatcatttt ctatttcaaa actgccttat
   110821 ttagcacaag gcggtcatct aatcaatgga caagcgattg ttggtgaagc tggacctgag
   110881 ttactgactg ccaagaatgg taaaaccaca gttactccat tatcagacga agaaaaacgc
   110941 aaagggatca gtggaagagt ttctggaggt agcattgaac agcatataca cattggcaaa
   111001 gtcgatgcaa acaatccatc tgagttagat cgtatgaatc gtaaatttgc taaagcaaac
   111061 aaacaggcta ttcgtgattt gggaggtgtt cctatttgag tcgacaattt atgaatccag
   111121 atgaaccaaa ttttatttgg aaaaatcgga atgcagtaat tgatatgaat tgcatcattg
   111181 aggcagagct tccagatatt atgccgaaca agcgatacga aacttacacg attcagggcc
   111241 gaagcggaga attgacggag acgtttgatg actatgaacc ttttgatttg gaaatagaag
   111301 ggatcactat tccacattcg aaactgagag aagtcaaacg atggctcact ggtaaaagcc
   111361 aattaatcac acataatgat ccagacaagt atctagaggc tatctgcagc atggataaag
   111421 aagtcccttt cgaaaacgag tggggcttct tttatacatt cgatgttact tttcgttgtc
   111481 agccactcaa aagaaaaatc ggtgaagcac ctaaaatttt tcattctagt gttatggata
   111541 tttttgatca tggtgacgaa attgcacatc cctatctgga aatagaatcg aacggaggag
   111601 atataacgat cgctgtcggt gataaaagcc taacaatact taatacgtta gctgcaacaa
   111661 tcgtggttga tactcagctt ggaaaagcaa tacaagaagg tttgaattta tttacgcgtg
   111721 gtgattggcc agtattgcag cctgaatgga atcaggtaaa aatatccgga aaatttaaag
   111781 aagtgcggtt ttggaatagg agtgtgtatc tgtgacgcaa gaatttattt atgcttataa
   111841 aaaaatgcca gatgatttga gcgtgaacgg agcgtcgtta gttgattggg aagatttgcc
   111901 agaaatcaat cgtgtgctga atggccaata tcgtttttat ggtaactatt caagaagcgg
   111961 acagtatcgc tcttatttaa aaaaaggaaa ttttataaaa gcgaaggtac cagatggatc
   112021 atggcaatat tttgaaattt ataatgtcaa aaagaatttg acctccgttt ctgtaacagc
   112081 tcggcatatt ggttttatgg ccaataagaa ctttattgtc aagtcgttta cggataacgg
   112141 caatgggtct cagatcatga ccaatcttaa aaacagtttg gcatttgatc aaaaatttaa
   112201 ctatctttcg aatgtaggga caacgcacca atttacagca agacaagtcg cgccagttga
   112261 agcaatcatt ggttcaaata acggtaatca aaatttgact ggcgtgacta gcgcggaact
   112321 tgatatggat aactatgatt tgaaactggt aaaacagatt ggatcagata atggttttcg
   112381 gattgatttt ggcatcaatt tagaagcaat cgaagaagaa attgatgaag aatcaattgt
   112441 caatagtttg tatttggttg gcggtgttcc tgataacgat tatgatgaag acaaagaacc
   112501 tatcgaatat ggttatttag aaattgatgg agtaaccaac gagaatcgac gtattgccaa
   112561 acgtgaaaac ggagattgta aaacagttga tgaattgatc aaatggggca aaaccttatt
   112621 tgacaatgat cgtatccatg agccaaaagc tactcataca gtcagtatgg tggcgttgga
   112681 acacacactc gaatacggtg acatgtatca agagctagcg tctttacact ttggtgatgt
   112741 cgctcatgtt agggcgaaag agctagatat cgagataaaa gagcgtatgg tcgaatacac
   112801 ttatttccca acgcttggga aatataagga tattgtttta ggtaatgatt tatcactgta
   112861 cacttcggca gtaaatactc aagcacaaga attgaaaaag aaaatcgaca atcgaacaga
   112921 aacgttagtt caaaatgtgt tgaatgcgac ggcatggatc actggtaatt ctggtgggca
   112981 tgtcgttttt cgtccagaaa aagctccatc agagattctt atcatggata ctccaaatgt
   113041 ggcgaatgca aagcgagtgt ggcgttggaa tttaaatggc ttaggttatt cagacaacgg
   113101 agtaaatggc ccgttcggta ttgctatcac atcaaaagga gaaattgttg ctgacttcat
   113161 caaagtagga acaataaatg cagaagtatt cgaaacttcc tttaatgctt atggtgatgt
   113221 gttaaaactt gtaaaaggca cgcttcaaat ttggaatgaa aacaaaaaaa tcatggagct
   113281 aaccaaaaaa ggtatggaat tctggaatag aaaagaatcc attggaacga tcggaacaac
   113341 agattcagca ggaaatccat ttccaaatgc tgtaacacct acacctcttg aggaaaattc
   113401 tttggtaata cgtacaaatg gcgatggaaa atacatttta atttctccaa ctgcagaaaa
   113461 aggatttgtg ttattaggaa acggaaaagc atattatttc ggtgatttag atattcaggg
   113521 aaaacttact gttagaggta aagaagtgat accaggacaa aatggcggtc catctggcgg
   113581 aggagaaaca cctggtgggt atcctgatga attaaaaaca gatgcagaaa agagagcttg
   113641 gagaatatac gatattttgt gtaataacgg atttacgaaa caatctgcat gtggaatatt
   113701 ggggaacatt caacaag
//
LOCUS       DACJGB010000002        89485 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.002, whole genome shotgun sequence.
ACCESSION   DACJGB010000002 DACJGB010000000
VERSION     DACJGB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.002"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..420
                     /locus_tag="IYI34_000134"
     CDS             <1..420
                     /locus_tag="IYI34_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAR0558039.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="IYI34_000135"
     CDS             complement(504..1226)
                     /locus_tag="IYI34_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAR0558040.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="IYI34_000136"
     CDS             complement(1216..1953)
                     /locus_tag="IYI34_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558041.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="IYI34_000137"
     CDS             complement(1965..2957)
                     /locus_tag="IYI34_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0558042.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="IYI34_000138"
     CDS             complement(2972..3715)
                     /locus_tag="IYI34_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558043.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="IYI34_000139"
     CDS             complement(3956..5278)
                     /locus_tag="IYI34_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR0558044.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="IYI34_000140"
     CDS             complement(5265..6230)
                     /locus_tag="IYI34_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558045.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="IYI34_000141"
     CDS             complement(6227..7765)
                     /locus_tag="IYI34_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAR0558046.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="IYI34_000142"
     CDS             complement(8181..8468)
                     /locus_tag="IYI34_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558047.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="IYI34_000143"
     CDS             8602..9270
                     /locus_tag="IYI34_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR0558048.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="IYI34_000144"
     CDS             complement(9561..10301)
                     /locus_tag="IYI34_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558049.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="IYI34_000145"
     CDS             complement(10431..11378)
                     /locus_tag="IYI34_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0558050.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="IYI34_000146"
     CDS             complement(11507..12271)
                     /locus_tag="IYI34_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR0558051.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="IYI34_000147"
     CDS             complement(12275..12664)
                     /locus_tag="IYI34_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558052.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="IYI34_000148"
     CDS             12799..13341
                     /locus_tag="IYI34_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAR0558053.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="IYI34_000149"
     CDS             13489..14121
                     /locus_tag="IYI34_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558054.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="IYI34_000150"
     CDS             complement(14255..14380)
                     /locus_tag="IYI34_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0558055.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="IYI34_000151"
     CDS             complement(14514..15407)
                     /locus_tag="IYI34_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAR0558056.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="IYI34_000152"
     CDS             complement(15589..16515)
                     /locus_tag="IYI34_000152"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAR0558057.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="IYI34_000153"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="IYI34_000153"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAR0558058.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="IYI34_000154"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="IYI34_000154"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAR0558059.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="IYI34_000155"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="IYI34_000155"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAR0558060.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="IYI34_000156"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="IYI34_000156"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAR0558061.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="IYI34_000157"
     CDS             complement(24786..25220)
                     /locus_tag="IYI34_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAR0558062.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="IYI34_000158"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="IYI34_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAR0558063.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="IYI34_000159"
     CDS             complement(26117..26992)
                     /locus_tag="IYI34_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAR0558064.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="IYI34_000160"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="IYI34_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAR0558065.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="IYI34_000161"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="IYI34_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAR0558066.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="IYI34_000162"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="IYI34_000162"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAR0558067.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="IYI34_000163"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="IYI34_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAR0558068.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="IYI34_000164"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="IYI34_000164"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAR0558069.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="IYI34_000165"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="IYI34_000165"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAR0558070.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="IYI34_000166"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="IYI34_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAR0558071.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="IYI34_000167"
     CDS             complement(35443..36210)
                     /locus_tag="IYI34_000167"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAR0558072.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="IYI34_000168"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="IYI34_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAR0558073.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="IYI34_000169"
     CDS             37323..37721
                     /locus_tag="IYI34_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAR0558074.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37958..38500
                     /locus_tag="IYI34_000170"
     CDS             37958..38500
                     /locus_tag="IYI34_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAR0558075.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="IYI34_000171"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="IYI34_000171"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAR0558076.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="IYI34_000172"
     CDS             complement(39286..39513)
                     /locus_tag="IYI34_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAR0558077.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="IYI34_000173"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="IYI34_000173"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR0558078.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="IYI34_000174"
     CDS             complement(40048..40761)
                     /locus_tag="IYI34_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAR0558079.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="IYI34_000175"
     CDS             complement(40764..41660)
                     /locus_tag="IYI34_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAR0558080.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="IYI34_000176"
     CDS             complement(41818..42660)
                     /locus_tag="IYI34_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR0558081.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="IYI34_000177"
     CDS             42792..43445
                     /locus_tag="IYI34_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAR0558082.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="IYI34_000178"
     CDS             complement(43508..44035)
                     /locus_tag="IYI34_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR0558083.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="IYI34_000179"
     CDS             complement(44055..45002)
                     /locus_tag="IYI34_000179"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAR0558084.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46900)
                     /locus_tag="IYI34_000180"
     CDS             complement(45014..46900)
                     /locus_tag="IYI34_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR0558085.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(47060..49138)
                     /locus_tag="IYI34_000181"
     CDS             complement(47060..49138)
                     /locus_tag="IYI34_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAR0558086.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="IYI34_000182"
     CDS             complement(49151..49426)
                     /locus_tag="IYI34_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558087.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="IYI34_000183"
     CDS             complement(49788..50243)
                     /locus_tag="IYI34_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558088.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="IYI34_000184"
     CDS             complement(50397..51605)
                     /locus_tag="IYI34_000184"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAR0558089.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="IYI34_000185"
     CDS             complement(51717..52049)
                     /locus_tag="IYI34_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAR0558090.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="IYI34_000186"
     CDS             52197..53072
                     /locus_tag="IYI34_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR0558091.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="IYI34_000187"
     CDS             complement(53154..53735)
                     /locus_tag="IYI34_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAR0558092.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="IYI34_000188"
     CDS             complement(53741..55000)
                     /locus_tag="IYI34_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAR0558093.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="IYI34_000189"
     CDS             complement(55002..56048)
                     /locus_tag="IYI34_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558094.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="IYI34_000190"
     CDS             complement(56283..56558)
                     /locus_tag="IYI34_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAR0558095.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /locus_tag="IYI34_000191"
     CDS             complement(56801..58111)
                     /locus_tag="IYI34_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAR0558096.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="IYI34_000192"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="IYI34_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAR0558097.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /locus_tag="IYI34_000193"
     CDS             complement(59657..60337)
                     /locus_tag="IYI34_000193"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAR0558098.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="IYI34_000194"
     CDS             complement(60386..61021)
                     /locus_tag="IYI34_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR0558099.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="IYI34_000195"
     CDS             complement(61087..62490)
                     /locus_tag="IYI34_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAR0558100.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="IYI34_000196"
     CDS             complement(62477..63508)
                     /locus_tag="IYI34_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558101.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="IYI34_000197"
     CDS             63539..63760
                     /locus_tag="IYI34_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAR0558102.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="IYI34_000198"
     CDS             complement(63925..64662)
                     /locus_tag="IYI34_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAR0558103.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="IYI34_000199"
     CDS             complement(64815..66155)
                     /locus_tag="IYI34_000199"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAR0558104.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="IYI34_000200"
     CDS             complement(66392..67915)
                     /locus_tag="IYI34_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAR0558105.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68292..68903)
                     /locus_tag="IYI34_000201"
     CDS             complement(68292..68903)
                     /locus_tag="IYI34_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR0558106.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69026..69151)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69233..69949)
                     /locus_tag="IYI34_000202"
     CDS             complement(69233..69949)
                     /locus_tag="IYI34_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAR0558107.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69955..70524)
                     /gene="scpB"
                     /locus_tag="IYI34_000203"
     CDS             complement(69955..70524)
                     /gene="scpB"
                     /locus_tag="IYI34_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAR0558108.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70508..71305)
                     /locus_tag="IYI34_000204"
     CDS             complement(70508..71305)
                     /locus_tag="IYI34_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAR0558109.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71275..71646)
                     /locus_tag="IYI34_000205"
     CDS             complement(71275..71646)
                     /locus_tag="IYI34_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAR0558110.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71665..72552)
                     /gene="xerD"
                     /locus_tag="IYI34_000206"
     CDS             complement(71665..72552)
                     /gene="xerD"
                     /locus_tag="IYI34_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAR0558111.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72648..73121)
                     /locus_tag="IYI34_000207"
     CDS             complement(72648..73121)
                     /locus_tag="IYI34_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAR0558112.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73226..74089)
                     /locus_tag="IYI34_000208"
     CDS             complement(73226..74089)
                     /locus_tag="IYI34_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAR0558113.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74285..76384)
                     /locus_tag="IYI34_000209"
     CDS             complement(74285..76384)
                     /locus_tag="IYI34_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAR0558114.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76604..77713)
                     /gene="rpoD"
                     /locus_tag="IYI34_000210"
     CDS             complement(76604..77713)
                     /gene="rpoD"
                     /locus_tag="IYI34_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAR0558115.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77737..79578)
                     /locus_tag="IYI34_000211"
     CDS             complement(77737..79578)
                     /locus_tag="IYI34_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAR0558116.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79760..82153)
                     /locus_tag="IYI34_000212"
     CDS             complement(79760..82153)
                     /locus_tag="IYI34_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR0558117.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82260..83120)
                     /locus_tag="IYI34_000213"
     CDS             complement(82260..83120)
                     /locus_tag="IYI34_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAR0558118.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83179..83700)
                     /locus_tag="IYI34_000214"
     CDS             complement(83179..83700)
                     /locus_tag="IYI34_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAR0558119.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            84020..84739
                     /locus_tag="IYI34_000215"
     CDS             84020..84739
                     /locus_tag="IYI34_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR0558120.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            84765..87356
                     /gene="mprF"
                     /locus_tag="IYI34_000216"
     CDS             84765..87356
                     /gene="mprF"
                     /locus_tag="IYI34_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAR0558121.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKC"
     gene            complement(87544..88731)
                     /gene="tuf"
                     /locus_tag="IYI34_000217"
     CDS             complement(87544..88731)
                     /gene="tuf"
                     /locus_tag="IYI34_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAR0558122.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            89099..89455
                     /locus_tag="IYI34_000218"
     CDS             89099..89455
                     /locus_tag="IYI34_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR0558123.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT        24683 a        18814 c        15349 g        30639 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt tataaacaaa cattttcgtc ttttaaaagg agaagaattt cacatttgca
    68161 aagaattgat tgatttttat gcataaatca aaaaaatact caaaacaaac aaataaaacg
    68221 tttacattat tgcatgtctt gtattttttt ccaaaaaaaa agaccaagaa atttcttgat
    68281 ctttttgata tttatttgac tgctgtatga cgttgttcga actgatgttg ttttcgactt
    68341 aaccatggaa gaagttgcgc atgcagcact aaaaagacag cactcacgat gaatcctttg
    68401 atcaagttga aaggaacgat cccaatcagt acatagtttg ctaaagacat gcccagcatc
    68461 tgtgttgcgt tcagccctaa aaacattaag taaagaggcg taataacgaa ataatttgcg
    68521 atactcataa aaatagttag agcgatggtt cctgtaatga cacctaaaaa tttgtttctt
    68581 cctttatgct gtttctttac actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt
    68641 gataagaaac tagcagtgtc tcctaccaaa ttatccggag aaaaacctgt agtcagcaga
    68701 tgtaatactg aacgcaagaa cgctgttgca actccggccg ctggaccgaa taagaacatg
    68761 ctgatcaata ccgagatatc actaaaatcg atcttcaaaa aactaaaagc cggcatgatc
    68821 ggaaaagcaa aatactgtaa aactaatgcg atcgcagcaa acatcgctac tgcaaccatt
    68881 ttctgtacac gggtgttctt catttgaaat cctcctgtta ggactcctct tcagcgaact
    68941 ttttgacgtt tctctccaaa tgaaaaaact ctattttcca gggaaaatag agttagagtt
    69001 atttgatcaa gcgtcgtcaa ataagctcta tcttctttat ccagactata ctgtcggtat
    69061 cggaatttca ccgattcagc tatttttttg caaaataggt cgtggactat aaccaccggt
    69121 cgggaatttc accctgcccc tgaagacgaa ccttatataa tttttgttta catattcatt
    69181 atacatatct attcaaaaaa cgcaacatct aacacttata aaaagtaagt gttcattttg
    69241 agtggttcag caaactgctg acttcttttt tgttcagttc acggtaatcc ccaggacgta
    69301 ggccttgtaa agtcagatct ccgtattttt cacgttttag tttttgcaca ggaagaccaa
    69361 ctgcttgaag catatttttc acttgatgat tacgtccttc gtggatcgtc aattccacga
    69421 tactgtggtt tgtcttagga tccactgata aaatttggaa tttagctgga gatgtttttc
    69481 gtccttcgat ctttattcct tttgtcaaag gtgcaagcat cgattttgta gcgatccctt
    69541 ttacctttgc aacgtaaact ttatccactt catgttttgg atgtgtcagg cgctgagcaa
    69601 aatctccatc atttgtcagt agcaagatcc ccgaagtatc gtaatccagt cgacctacag
    69661 gatagatacg ttcttttacc atcggcaaaa gatccgtaac aacttttctt cctttgtcat
    69721 ctgcaacagc agaaataact cctttaggtt tgtacaataa atagtaaccg tattcttctt
    69781 gataaattgg gacttcatcc acttccacag tgtcatgttt cccaactttt atccctaatt
    69841 cccttacaac ttgtccgttt acttttacac gtcctgcaag gatgaattct tctgcttttc
    69901 gacgagaagt gatgccagcg tgggcaatta ctttctgcaa tctctccatt acaattattc
    69961 tcctttattc attccatcaa agaatagatc tggtgacacg tcttcttcaa gtgaaatttc
    70021 catttcttgt gtatcaggca gttcttctaa tgatttcaaa ccaaaataat ctaagaagta
    70081 gccagttgtt ccatataata tcggccgacc tggtccttct actctacctt tttcttcaat
    70141 cagtttatgg gcagctagac gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc
    70201 gatttcagct cttgtgattg gctgtttata agcgatgatt gctaaggttt cgacggcggc
    70261 ttgagataat gtgtttgatg tcccttgcgc atatcgctta agtaagggag ccagctcttt
    70321 tttagtcgtt aatacataat ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt
    70381 ttgagtgtaa gattctttta attctttcac taaacttgtg acttctgctt cgctgagatt
    70441 taacagtccg cttaattctt cattactgat cccttcctcg ccaacaataa aaagaagggc
    70501 ttcaagttca cttaatttcg tcatgttcgg tatctcctac tgctggataa agcataattg
    70561 attcatagtt ttctttttgt tgcgcaatga tctgcccttt tttcatcaat tctagcaaag
    70621 ccataaaagt agtgacaact tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac
    70681 agccatgcgc atttcttttt atacgctctt cgataaatac cattcgttct tcgatcgttg
    70741 tgtcatcggg cgtgatcgtt ttttccactt tttgagaaaa tttcttacgt tctaagattt
    70801 tctgcatggc acggatcaaa tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag
    70861 catccgctaa cccatcgatg ttcataggtt cttttgtgta ataaagactt cgttcctctg
    70921 ctttttctgt cagtctttca gctgcatatt tatatttacg gtattctaaa agctgagtga
    70981 ccaattcttc tcgaggatct tcttcccata attcttcatc gtctgtgacg aattcttgga
    71041 caggaagcaa tgtcttgctt ttgattgcca tcaatgtagc agccatcact aagtattctc
    71101 ctgcaacagc tagctcgagt gttttcattg tatggatata attcatatat tgctccgtga
    71161 tcgtagcgat cggtatatca tatatatcga tttccatttg ctgaatcaga tgaagcagca
    71221 gatcgagagg accctcgaat acatctaatt taatattgat ctccgtcaac tcattcactc
    71281 tttcttcttt ttctttgcta gccattttcc aatgtaaggc tctgtgattt cagttgcacg
    71341 gatggagaca tccgggtact tttcctgtaa ttcatctaac atttgaaagc ccatattctc
    71401 accgcgataa gacggattca aagaaatgtc atggatgatc agctgatcgt tcgttgccca
    71461 gtaaatacca ataatccctt gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc
    71521 ataatcgtct tcagattcgt acgtatcaat ctccttcaac aaagaagttt tacctgtcag
    71581 tttcttatgg aaactcaata gtcccatagc aattttacgg ttttctttcc ggtaatgtgt
    71641 taacatcttc tcaccttcct atgactaagc ccttggaaaa tgctgcttat aaacatccgt
    71701 cattcttttc ttcgtgatat gtgtataaat ctgagtcgta gagatatccg cgtgacctaa
    71761 caattcctgt actgtccgca aatcagctcc attttctagc agatgtgtgg caaagctatg
    71821 ccgcagcgta tgtggtgtta cttctttgta aatcccagcc tctcgtacta actgcttcag
    71881 atttttccag attccttgcc gagaaagacc tttcccatga ttattgacaa atacatgcat
    71941 ttcagatgcg tcctttacaa gtaatggacg tgcttcgtct aagtagcgtt ccagccattg
    72001 gatcgcataa tcacctaaag gaatgatccg ctctttatct ccttttccaa ctgtctgtac
    72061 cagtccaaga gaaagatgaa gatctcccaa tttcaatcca atcaattcac tgacgcgcat
    72121 cccagtagca tacatgactt ccaagattgc acgatctctt atccctaacg tttttgtcgt
    72181 atcaggtgct tcaatcagct tttctacttc tgtcagagtc aatgtacttg gtaatttctg
    72241 tgctttcttc ggcgtatcga tatgttgcat cggatcatga tcagtgatac gctcttgccg
    72301 taaaaactga tggaatcttc tgagacttga gatcatccga gtaacagttg ctgtcgcttt
    72361 tttttcttca tgcaacttct ccagaaaacg gatgactaaa aagcgatcga catcctgcca
    72421 tgaagcaatt ttctgctcct gtagaaaatg caagtactgt tccaaatcac gttcatagct
    72481 ttgtcgtgta ttggcggaca atccccgttc aatattcaag tagtggagat aatcgttgat
    72541 ttgctctttc ataataatcc ccctcatgcc tctctatcat atcagatacc gcttcattct
    72601 gatagtcttt ttccataatc tgagcaaaga aaaagccgtc tagacactca gacggactgg
    72661 tttctttttt ctttttcata gcattcctga cagattccat ggaacgttag ccgatgatct
    72721 tttactaaaa aatgataacg ttcttctact acttgctcca cttcaagtaa aagatcctct
    72781 tctacttcct cgatcgtgcc gcagttcaag catagtaaat gatgatggaa atgtttggcg
    72841 ccttcttttc tcaaatcata acgggctaat ccatcattaa aacttacttt atcgacaatt
    72901 ttcaattctg tcaagatttc aagggttcga taaacagtag caagtccaat ttccggactc
    72961 ttttttttca ctaaaaagaa gatttcttct gcagataaat gctctttctc attttccaaa
    73021 agcacaagca gagtagcttc tctttgcggt gtcagcttaa accccgcttc atgtagctgc
    73081 tgttttgttt ttttcatcgc cgaaatagca ttcatgacca tttactcaac cccaacaatt
    73141 tataataatt acaaataaaa aaagatataa cactatgtta attaaaatta ttataagcta
    73201 gttaagaaaa acatgcaagg gattctcaat tagatttttt gactagtttt gtcacaccgt
    73261 tttcttgtgt aatcaatcct tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt
    73321 tgctgatacc aaaagctttc atgatttctt ctggatccga tttatctgta aagttgattt
    73381 gctgatctgc agaacgttct aaaatggtta aaatcatttg tgcatcatct gatatagctt
    73441 catatgctct tggcttcaat gaaagattca gtacaccgtc tggtcggaca ccaatcactc
    73501 gaccttttat ctgctcgcct agacgaggtt cattatatcg ttcagaagga tggataaacc
    73561 caagatagaa atcttccgtt aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc
    73621 ctatcacatc tttgtttttc atttcttctg ttccatattt gcttaaagat ttgaatattt
    73681 tttcatctgc taaactgccc caaatacggt cttttgcatc aactctaagg gaaatcatca
    73741 agcgatcccc ttttttaggc catagctctg tcattgtcgg caattcatca agagatacga
    73801 caaagtcttt atctggaaga ccgatatcga caaacgcacc taaatctttt cgtgtagcag
    73861 taacgattcc aaaagcgtaa tgaccgatac ggcttttagg aatttgtgta gtgatccgat
    73921 tttcatgttt ttgattttgg taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac
    73981 cttctgattt gcttaagcca aaggtttgtc cgtttttttg gacaaagtag tttttttcat
    74041 tttcatcaat aataatacct gtaaatatag ttgctaataa atcgttcatt tttttctccc
    74101 tctatagtga aaggttttct taatacattt ctttgctatc ataacataat gttatctact
    74161 tgaatagctg agccttggtt tggaaaggtg aaaaaaggag tatgacaaca gttgtgtcat
    74221 actccccttt ttcgaataaa cggtgttaaa cggcaggctc acagcctctt tcatttgcct
    74281 aaaattaatt gctcccattt cctccgaacc atccagtttt acagttgatg gttacgatgt
    74341 aaaatggcaa agcactttct ccaatgcctt tcatcaatgc agattttgtt gaagtggcat
    74401 atagattata atagccttcc ccattttcga ttctggcttt ttctaatctg taacctcctt
    74461 caacgatata tccacgttgg atacatgtag caatcacttt gctttggaca cctggtgccc
    74521 acgtccatgc tggattagat gcatcggcaa tatcacttgc tctggcagat ttgattggct
    74581 gattcgctga gtttgaagag gaaggggtcg aggatgccgc ttcttgctgt gctgcttgtg
    74641 cgaccgcagc ttgttgagcg gcttgagctg cagctgctgc ttgagcggct tgttttgctt
    74701 gttcttcagc tgcttttttc gcttcttctg cttttttctt ttcctcttct tcttttttct
    74761 ctttatcagt gagcatctta tctacttgtt tcaattgttc attcaactga gcagatagat
    74821 ctgtattttt cactttttct actgcttctt ttgccgtctt gtattggtcg cgtgttgctg
    74881 aatccactac ttttccgtct ttaaagacta tagctgtttt ttctttagct gtttgcaatt
    74941 cagcatattg atttttagct tctgtttgtg cttgttgaat caattttcca aaatctgtgt
    75001 tatccgtttc gattgcttgg atctctttat ctgctttcaa cttgatgtca tctgctagat
    75061 gatcatcgac gatggcaggt gctatgaacc actcattcac tttttggata tctcggatct
    75121 tagattggat atcttggtaa gttgtttcta actctgaata tcctttttca ttcttaagac
    75181 tggataaatc attttttagt tttgataaat cttgatgaat catatctgca cgaataaatt
    75241 gatggtgatc atctgtataa aaggcagcaa gtttctcatg aagttcttcc atttgttgct
    75301 gtctttttgc ttctgctgct acttcggctt ctactttttg atgatggtca taataagccc
    75361 agcttcctcc accgagtaga agtactgctg ctaaaacgag ataaggtact ttttttgatt
    75421 ttttcttttg cgctttatct ggatctgttt tttctaagtt tgattcttga tgtgtttttt
    75481 cagtatctgg cacgattgtt attgatttcg cttctgcttc aaacagctta ggctggttct
    75541 cttctttttc tatttttgct tcaagatcag taccctcttt gcttacagat tgattttcag
    75601 catcggattc actatttatt tcatcgggct ctgttgactt tttgtctgtg ttttcgagta
    75661 tttctttttt ctctgcttcc ggcagtttat tcgttttttc cttttctaat tgtttttcag
    75721 taaatacttc agttttttct gtgatatccg tttttacgtc ttttattttt actgtttctt
    75781 tacctgtggc gttttctcta tcagagtctt tttcagtttc ttgttccttt tctggctcta
    75841 ctttgttttc ctgtgcaatt ttttcactga tcattttgag cagctctttc gcgtgctctt
    75901 cttttgtatc ttcacgatgt gctttgatat aagcggatag tatcgaattt tcttcctcat
    75961 tatcgacgat ttctttcagt tcagctactc ttcttttttg ttttgattca agcgtttctt
    76021 cttgattctt tcctgattct tccgaagtct catgaacagt tgctttcttc tgttcatttt
    76081 tctctatatt agttacttga tcgtgagcag gtggttcttc gtcttctggt tctgcttttt
    76141 cgaataattc cattacagat tctagaggta aatctttcaa ttccgaccat tcgattggat
    76201 cgtttaattc tgtatcagga aagactgcat cttcagttga tttatcctgc atagctgcat
    76261 ctgtatttgt ttcttcaatt gattctttta tttgctgatc ttctgcagtt atttgatgac
    76321 cgcagtctgg acagatagta ttctgctcca tttttttcga tccgcaattt ggacattttt
    76381 tcatcactta gcgatcggct cctttactag gtttacgcta ccatttaatc tatcatattc
    76441 tatacgtcac gccaatagct tatttttagg atttacttaa aaataatgaa tgaatttcct
    76501 tcctatagaa aaaaaagctt gagactgcgt agtctcaagc tttttttctt ttaaaataaa
    76561 ccaaacagtt ttactgattt ctcttctggt caatagtcca cgcttattct aagaaatctt
    76621 ttaattgttt tgaacgagaa ggatggcgca attttcttaa tgctttagcc tcgatctgac
    76681 gaatacgttc ccgagtaaca ccaaatactt tccctacttc ttccaatgtt ctcgtccggc
    76741 catcatctaa accaaaacgt aagcgaagga cattctcttc ccgatcagtc aatgtatcta
    76801 gtacatcttc caattgttct tttagtaact cataagctgc gtgttctgct ggacttgttg
    76861 cttcctggtc ttctatgaaa tcacctaagt gagagtcatc ttcttcaccg attggcgttt
    76921 caagggaaac aggttcttgc gcgattttca agatttctcg gactttttct gttggaagat
    76981 ccatttccgc accgatttct tctggcgtag gttctcttcc aaggtcttgc agcaattgac
    77041 gttggatacg tatcaattta ttaattgttt caaccatatg aactggaata cggatcgtac
    77101 gtgcttggtc agcgattgct cgagtgatgg cttgacgaat ccaccatgta gcataggttg
    77161 aaaacttaaa tcctttgcgg tagtcaaatt tttcaactgc cttcatcagt cccatgtttc
    77221 cttcttggat caagtcaagg aactgcatgc ctcgtcctac ataacgttta gcgatactta
    77281 ctactaaacg aaggtttgct tctgccagac gctgtttcgc ttcttggtcg ccttcttcga
    77341 ttttcaatgc taacgctact tcttcttccg cagttaaaag ggagacacgg ccaatttctt
    77401 tcagatacat acgaaccggg tcattgattt ttacaccggt tggcgcagaa agatcttctg
    77461 tttttgcttg ttcagctttt ttctcagcac tcttcaagct gtgaattgat ggatcaccat
    77521 tttcatccac cacactgata cctgcgtctt caatcttttg gatcaatttg tccatttcat
    77581 ctgcattcaa agtgaatggt gtagcaagct gattggataa atcatcatat acaacttgtc
    77641 gtttgggttt gttttctttg atgaaagctg ctactgctgc ttcatacgtt tttttatctg
    77701 ttccatttgc cataaaagaa ggcccccctt gagtagctat ttggcttgtt ttaactgttt
    77761 cgttaaattg atgatttcaa tcgtcaattc aagctcaagc tgtttgtttc cagttcgtct
    77821 ggcttcttgc tgcatttgtt tttttaatgt tatttcatca gctaagccag atttgctgat
    77881 cacatctagc aagtcttgga tttctcgtgg cgaactttct tctgccaccg gtaaatattc
    77941 gatttccacc accaaccgcc ttaaattttc atcttgcaaa aagtctaaaa actctgcaag
    78001 gttgaattca taatgttgtg agacatatgc atcgaataaa aaataaactt ctgcatatgc
    78061 atcatgaata aactgaaatt ctcgttcttt taaaatatta cggacagaaa cttctttaaa
    78121 tgcacgatat aatagaatct gctctgcctt ttctacttgc gtcaaaggtc tctttttccg
    78181 tgcagtttct tgtctcgtaa cctgagtgtg ttgactttcc tgaggttctt gacgattttg
    78241 ctgatttgtc cttcgaagat cgcgcagttg tttttgtatc gtctctctgc cgatctgaaa
    78301 ctctgttgac agttgagata gatacatatc ttgttcgatt ggtgaaacaa ctcggctaag
    78361 ttcaactagt aattcttcga ggtattcgat cttgtctttt tcattatcta aatttttccc
    78421 ttgttttaaa taacgtgatt taaaagtaaa gactgtttcg cgcccatgag ccactaattc
    78481 ttggaaagat gcttctccat acttacggag atattcatca ggatcaagct tttctggaac
    78541 gactacgaca gaaagtgcta atcgactgtt ttgccgaagc aattcaatcc ctcgattggt
    78601 tgcttcaaaa cctgcgttgt ctccgtcata gcaaaagacc aattctttcg tcagcttttc
    78661 catttgttgg atctgttgag ctgttagact tgtccccata gaagctaatc cagttttgat
    78721 accagcctgc catgcagcaa tcacatccat aaacccttcg aacaacaaaa tttctccgtt
    78781 tttccggata tcgcttcttg ctttatcaag attaaataaa acttgtcttt tattgaacag
    78841 ttctgtttct ggactattca agtatttggg ctgatctttt tcctgccctt tttcaggtgc
    78901 aagccagcgt cctgaaaaac cgatcgtctg gccttgagaa ttacgaagtg gaaacatcac
    78961 tctttgataa aaacgatcag acatacggcc atcatcatgc gtgacaaaca atccagagcg
    79021 ttctaacaat tcatttgaaa ttgattcatt cttgaatact tgctgaagaa aagaacgctc
    79081 attaggtgca aaacctatgt tgaattcttc aattaattct aaagacaacc cacgactttg
    79141 caaatattct aaagctggct gtccagcttt tgtatgcaat agcatgtgat gatagatttc
    79201 cgctgctttt tcatgagcag caatcagttt ccccgtagca gattcaggat tttggacagc
    79261 tatagtacgc tgacgccatt gatcttctaa cggtatctgt tcgaagtctg ccacttttac
    79321 cactgcttct ggaaaactta gaccttctag ttcttgaaga aaagtaaaca cgtttccgcc
    79381 tttaccacaa ccaaagcaat gaaaaatctg cttgtcttct gcaacagaaa aagacggtgt
    79441 acgttcttca tgaaaaggac acagccccaa gtaattttta ccagactttt tcaactggac
    79501 atattgtcca atcacctcaa cgatattcgt ttgactgcgt actgtttcga taacctcttg
    79561 aggaatccgt tgagccattt tttcaccccc tgctctattg atatcttttg ggtttaacag
    79621 aagatatttc aggagaataa ccggtaaaaa atcgcactaa ctgaaatcag tgcgatttta
    79681 aaggacacaa ttatacatgt ttgattgtat catatttcat tgctttttca agtataacac
    79741 atttctatct cattaagcat caatttgcta ctcttttcag taactgattt tgaatatcag
    79801 ccttccagta caatactcga agaacaatat ttacgagcat gaatacaaag aagtatccta
    79861 cgctctctcc caaccctaag tttacttcca aaattccatg gaacagcatt gcagctacat
    79921 atatccctac gatcgtcgtt actgctagga agatgcgcaa tgggttgaag ggataacaag
    79981 cttttacaac tgccatgcaa ctgatcccaa tcagcaagta atacatcaaa gtagtagttt
    80041 cagcatttcc ccatgcgaat gtttttccaa gtagatacac cgcaatgata ttcactacta
    80101 caagcaaggc atttggtaaa gcattaataa gtgctgtagg caaataacgg taagtgattt
    80161 ttcttttatc ttgttcaaaa gataaaaaga aggaaggata cccttcaatc gctaaatcaa
    80221 tcagcgtgat ttggataggg ataaacggaa atccaattgc tgtaacggca catataatcg
    80281 ataaaataaa tgaataaatc gttttgataa aaaagatgcc tgagactttc gtcacattgt
    80341 tgaccactcg gcgtccttca aacaggactt ctggcaatgt agtaaaatca gagtccaaca
    80401 ataccagatt cgcaatctga cgtgttgctc catcaccttc tgccatagca atgctgcaat
    80461 ctgcttctct taacgccaaa acatcattca caccgtcgcc tgtcattgca accgttctgc
    80521 cgctttcttt caattcattt accagcaatt tcttttgctg cggactcact cgtccaaaaa
    80581 ccgtatagcg atgagcagct tctcgcactt ccatttcttc agtaatttga gaaagatcga
    80641 catacgcatc ataattcggt aatcctgcac gtctagcgat attggagaca gtaaaaggat
    80701 tgtctccaga aatcactttc agatccacac cttcctcatg aagataagct agtgtttctt
    80761 gagcgttttt tcggattgga tcatcgattt caaacagagc aaccggctca acaaatgctg
    80821 gtttttcttc gttcaattgc tgatttggag cgatccccaa cataagtacg cgatagccat
    80881 tcgcttgggc tgtttttaga ctgtccggaa gccgatcttc ttccaccaaa cgttcaggcg
    80941 ctcctacgta cacgactccc agttctggaa attccatcgc gccccatttg cgatcagaag
    81001 aaaatgcgac tttgtttgtc acatcatacc gattagaaag ctcaaaataa tctctcatag
    81061 cccgcatagt gatgttgtta tcactgcttt ctgccagata cgtccccata atttctggga
    81121 tggctttctc gtacagcgga tgtagtgctt ctacccgctg gatactcatt ttcccttcag
    81181 taatggtccc tgttttatcc aagcataatg tatcgacatg ggccagcgtt tcaattgaat
    81241 acatgtcttg aacaaggatt cgtttttttg ccagtttggt tacccctgtc gttaatgcaa
    81301 tacttattag caataccaaa ccctttggaa gcatgcctaa caaagctgct gcagatgcaa
    81361 taacagaaat tttgatagaa gcatcgcgga tgaacagtgc ttccaaaaat agtatgatcc
    81421 ccaaaggaat aatcacgaaa ctagtgaatt tggatacttt gcgaatcgaa tctacaagtt
    81481 cagaacgaat agctttgtga gtctttgcct cgctagtcaa gcggacagca taattatctg
    81541 cacctactcg gataacttca gccatgacag aaccgcttgt caaaaagctt ccagaaagca
    81601 attcagctcc agtttctttg acaatcaaat cagattcgcc tgtcaaaaga gcttcatttg
    81661 cttctgcccg tccagatata acctgtaaat cggctggtat ctgttctcct gctccgattt
    81721 ttaccaaatc tcctaatacc agctctttag tagaaatcga ttgttcttta ccgtcccgga
    81781 tcactagtac ttctgattgg gaaacaatcg ataatttccg tacaagattt cttgcatgta
    81841 tttcttgata aattccaata acgatattga cgacaataat tgctaaatac gccatgttgg
    81901 aatacgcacc tacaaataac agacaaatcc caataccaaa gttcagaaaa ttgaataacg
    81961 tcaatatatt atcgctgaat atttgttttg tactttttga tgcatcttcc tggtagtcat
    82021 tttgctggcc agaagccatt ttctgaacta cctcttgctg tgtaagtcca ttcattttct
    82081 ttttctccat tttccgccaa ctttcaaaaa taatcacgtg tttaagtcta gcgtgttcca
    82141 agattaaaaa caacttgttt ttataaaatt tatagaaaaa cacacgaatg attttacaaa
    82201 aaagatgcca acctttttgt ttgaatcacc cgtgtgctgt ttttattatc aatctatctt
    82261 tacttatatt gttcctccaa acggctcatc caccaaccta gtgctgtacc ggctataaaa
    82321 agaacaggac aagctagtac cccagcaact cccaatcccg cgtaatcggt aggaatgatc
    82381 ataatcgttg aagcaaaaac tacacctaaa atgaaatgga atagcttgct atatgccttt
    82441 gagaaaatgt aatccatgat ttttgacaat ccaagaacac aaagaatccc accaattccg
    82501 atcggcacaa tcacttcaag tcgtacttct ttaaatccgt cagccatcgc tttatacaat
    82561 cccatataca ccaaaaaatt agaagggctc aatcctggga cgatcatacc caaaccaatc
    82621 aatccgccgg caatcagcca tgttccaaaa ttctgatcga cttgcccgtc aaataattga
    82681 gcgccgtata ataaaaacaa ataagccaga acaaaagtaa caaccatgat gatcacatca
    82741 gaagtatctc ttcctttttt ccctgcttct ttccataacg caggaacagt tccagcgata
    82801 catccgacaa aaaaccataa tatcgttgtt tcatacgcac ctaataaaaa actgactaaa
    82861 aaagaaagta gaaagatgcc tgtcactccg cctagaccaa caggcagaaa aaacaaaaca
    82921 ttttctttga aattttttgt aaggtgggct aaaaaagaaa tcagccgctc atacatgcca
    82981 aagacagctg ctaatgcacc accgctgact cctggcaaga taaatcctga accgataaac
    83041 attcccttca caaaccgtaa catccagtct ttggttgtgt accccacttc ttgattttcc
    83101 attttttgcc ttttttccac tatttgcgta ctccttcttt catttaattt tattcatgat
    83161 tgaacatttc ctgctatttt acttaaagac tttttcatag gtctctatta ttttttcatt
    83221 cattttgaca atatcaggat aaaacatgtt acctccatta tgatagagat agccgccatt
    83281 gtaaagcaag gactggatcc gccaatatcg gtattgtttc ccttcctcat ttcctaaaag
    83341 tgggctcagc acatctttag aatacgcttc tgctaattct actgtgtttt ctccgccatg
    83401 attttttaca taggcaatat aatctttccc aaaattatat gcttggacag ctgtccataa
    83461 atcgcatcct tgtttctttg cttctactat catttccgcc aaatatttga caccttgatc
    83521 aatactgtca gctggattat caatgatctc agcttctcca tgcagacttt ccgagctctg
    83581 catgagatct gtcccttttc cttttgattc ggtcaatatt atcgccttga tcaaattctc
    83641 gtagttttcc atatgataat tttttgcagc tgttgttatt tctatttgat agctttccac
    83701 tcgttttacc gaacgataag ttcttcctca taaaaacaac accgcaccaa taaccgtcag
    83761 aatgatcatg taaaaaagaa tacggataat agataatcgt tttcgcttca tttcatcacc
    83821 tagttatttt gacatctttt tttatcttac tatttcttta tcttcttggc tatttcttcc
    83881 ttgatttctg tgtacgcctt aagaaaaaat gaattttcct gtattttttg acatcttacg
    83941 aaaatgatcg ttgtttttca gtaaaaacaa ttgcccaatg actgatataa tcgttatact
    84001 ttacacagta ggtgattatg tgaaaaaaga aaagaaatat gcaaaaatgc cagatgggag
    84061 cgaaatttac tatgaaaaga gcggtcaagg cttccctctc tttttactgc atggcaatga
    84121 cggcagcggc cgcttttttt cagaacaggt ccctgtactc gaacgctact ataccgtgta
    84181 tctagtagat agccgaggac atggtcgttc gacaaacgaa gcatctatgt tgaattttca
    84241 gttaatggcc gaggatctga atacgatcat gttgctggaa aaaatcgatc aggcagattt
    84301 tcttggattt agtgatggag caaatctagc actggtattt gccagcagct tccctaaaaa
    84361 agttcatcga ttgattttaa attctggaaa cacgcttgtt aaaggcgttc gtttttctgc
    84421 cagagtcatc agtaatattc attatgcatg ggtatggtta ctctctttat ttcgcccaag
    84481 tcttcgcaaa aatcttttag ttatcaaact tttgctgcat gacatcgggc ttacagaaaa
    84541 tgatttaaaa aaaatcaact cccccacttt gatcatcgtg ggaaaaaaag atgtaatcaa
    84601 gctaaaacat tctttgtata tagctaaaac tataccaaaa gcttcctttg tgcttgtgaa
    84661 agaacaagga catgaattag ctagaaaaga tcctgaacgc ttcaatcgag aagttcttca
    84721 atttctttct gaaacataac tattaggaat agaaggtgat ttatttgtta aaaaatacca
    84781 tacaatggct gaaaaaccac ctaggactgt tcaaaacaat ctttttgatt tctgtcattg
    84841 tcattattgt cggtgaattg atgtccatcg gtaaaactct atctatccag caattaggag
    84901 aaacgtttgc cactatacct gtatggaaaa gcggactaat gctattgatt ggtcttatct
    84961 ctgtgttacc tatggttggt tatgatatta ttttgaatcg gatgcttgaa caaaagcaga
    85021 atcctcgtta cctgtttgag acaagctggc tgatcaatac gatcaacaat attgccggtt
    85081 tcggcgggtt tgttagtgtg gggcttcgct ctgaactata tggacaaaag aaagagagca
    85141 gaaacgtgat ccaagcatta tcgaaagttt tcttgttttt gatggctgga ctttctattt
    85201 acagtctgct ttctttttta cttgtagtct tcacgccagt tgctcctttt ttgaagcaat
    85261 attggatatg gttgatcggc ggcggtctat attttcctgt cgtcttttta tttactactt
    85321 tcaaaaagaa aggattcctt ggtggtacct cacttaaaac tcaaggacag ctattgctag
    85381 tttcattgtt ggaatggacc ggtgtcctgt ttagtttcct ttctgttggt tatttaatgg
    85441 aaatccggat tgatctgtgg caaacaattc cattattcat tgctgcttca gtgataggaa
    85501 tcgtgtctat gattccagga gaaattggga gtttcgatgt aatgatgatt atcgggttgt
    85561 ccgctattgg tgtgccaaga gaaatagtcg ttgtgtggat cttgctctat cgcctgttct
    85621 actacatcat tccgtttttg attggaatcg tctttttctt caaaaatatc ggttccactt
    85681 ttgatcagcg ttattctggt atccctaaac aattagcaac agaaattgcg cataaaatcg
    85741 tggttgtctt attatacttt tctggaatca tgctagtact ttctgccact atcccgcaag
    85801 cttttacaga gtttcgctgg cttcatagtt tgaacccatt gaaatttcat tttattatcc
    85861 aatttcctag tatactattg ggatttcttt taatcattat gggcagaggg atcgcagcac
    85921 gtgtgaaacg agcatatttg cctacaatct tcttgattgt tctagcttta ctttatgttt
    85981 tattaagtga ttttagtttc acaccggtgg ttttcttgtc tattctgctt ctgatcattc
    86041 ttgcttctaa aaacgaatta tttcgggaac aattgatcta ttcatgggag tggcgtacga
    86101 tcgatggaat cattattggt gcactgtcct tgctttacat cgttattggg gtatacaatc
    86161 tgccggattt ccctcatcgg cgtcaccatt ttatttcctt tttcttattt ccttctgaaa
    86221 agatttggtt ttcgggattg ttggccatca tcgctgttag tttcatgatc gttctatttg
    86281 ttcatttcct tcaaggagaa aagaaacaga ttggcgaagc ctttaatgaa gaaaaggccc
    86341 tgaagatcct tacaacttat ggaggaaact ccgatagtca attgatcttc ttaaaagata
    86401 agcggatgtt tgcctatgaa aaagacggtg aaccaactgt tcttctccaa ttcgcttgct
    86461 tcaataataa atgtattgta atgggtgatc cttctggtaa gaaagaagat tttccagaag
    86521 caatcgaagc ttttatcgaa gaaacagatc gactatgcta tttaccagtt ttctacgaaa
    86581 caagcgaaga gattgttatg attttgcatg aattcggcta cgatttcatt aagatggggg
    86641 aagaagctta tgtagacctg aatagtttta caacttcagg gaaaaaaatg aagggcactc
    86701 gtgctgtctt gaatcggatc gaacgagaag gatttacatt tgatgtctta caaccaccat
    86761 tttctactga gcaaatgagt actttcaaaa atatctctga taattggttg ggttcacgaa
    86821 aggaaaaagg gttttcttta ggctttttct ctgaagatta tctacaaaga gcgcctattg
    86881 cagttgtcaa aaatacccaa gaagaaattg tcgcttttgc aacgatcatg cctacttaca
    86941 cgaacaataa agtaggaacg attgatctga tgcgatatga ttctaaaaaa gcaccttctg
    87001 gcagtatgga tttcttgttc ttgcatttgt ttgcctatat gaaagaagaa catatccaat
    87061 ggttcaattt agggatggcc cctctttcaa atgtcggaac ttccagaaag agttttttac
    87121 aggaaaggat tgcatacttg gtttacgagt ttggttctca tttttattct ttccatggat
    87181 taagagaata taaaagcaag tacgctacaa actgggtctc acgttatact ctatattcta
    87241 gagatagttg gattgcatat gttatgattg ctttgctgat tattgataat gcgccagttg
    87301 agcagacaca aggtagaata agtggtttga aaaaatggat acgaagaaag tgttaaaaac
    87361 tgcataccga tagatgaaca ctctcatgat gaataaaaga gactgaagta aaacgaaagt
    87421 tcatgcttct acaatctctt cttatacaat tgaagaacaa agagcggtcc aaacagaaat
    87481 cctgtttgga ccgctctttt attttaaaca tttttcatta ccaaaatatt cgagtcgcgt
    87541 ttcttatgct tcgatctctg taacgatacc tgaacctact gtccgtccgc cttcacgaat
    87601 tgagaatgtc gttccgtttt caacagcgat tggatggatc aagtcaacat caatcgtcac
    87661 gttgtcacca ggcatgacca tttctgtatc ttctggcaat gtgattgttc ctgtaacatc
    87721 agttgtgcgg aagtagaact gtggacgata gttgttgaag aatggagtat gacgtccacc
    87781 ttcttctttt gtcaacacgt acacttctgc tttgaatttt gtatgtggag taatggaacc
    87841 tggtttagca agtacttgtc cacgttcgat atcgtcacgt tggatccccc gtaacaacac
    87901 gcctacgtta tccccagctt ccccgtaatc taacgtttta cggaacattt ctacacctgt
    87961 tacaactgct ttttgtgttt caggtttgat ccctacgatc tctacctcat caccgacacg
    88021 aacagcacca cgatcaatac gacctgaggc aacagttcct cgacctgtaa ttgagaagac
    88081 gtcttccact ggcaatagta atggtttatc tgtatcacgt tctggtgttg ggatatattc
    88141 atctactgta tccatcaatt ccataatagc agcttcagca tctggatcgc cttgcaatgc
    88201 ttttaatgcg gaacctttga tcacaggagt gtcatcgcct gggaaaccat attcgctcaa
    88261 taactcgcga acttccattt ctaccaaatc gatcaattct tcatcatcga ctaaatcaac
    88321 tttgttcaag aaaacaatca ggtattttac accaacttgg cgtgacaata aaatgtgttc
    88381 acgtgtttga ggcatcggac cgtcagttgc agaaacaact aagattgcac catccatttg
    88441 tgctgcccct gtaatcatgt ttttcacata gtccgcgtgt cctggtgcat caatatgcgc
    88501 gtagtggcgt ttttctgttt catattctac gtgtgcggta ttgatcgtaa tcccacgttc
    88561 acgttcttct ggagctgcat caatactagc atagtcttga ggatttgcta ggcctttttt
    88621 gcctaatact gtagtgatcg cagcagttaa agtagttttc ccatggtcaa cgtgtccaat
    88681 cgtaccaatg ttaacgtgtg gtttacttct gtcataatgt tcttttgcca taatttacaa
    88741 acctcctaaa taatatagat cactgaaaaa tttcagctct caccttttta ttatagtctt
    88801 tttttgacta aatcaaaaag aatacgtccg acttactttt tgtaagattc gaactaagct
    88861 tattatacgt caatattggt caaagtcaac taagagcact cgtctgtctt atacgcgaaa
    88921 aaagtacctt ttataaataa aaaaatgtgt tttttgagaa aaacttcaag atagtcctta
    88981 actttctttt gaagcaataa gaacaaaatg aggaaaagtt taaaattcag ctgttgccaa
    89041 caactttcaa tagacgaaat cttctatttc gttaataata aggaaagagg tgaagtacat
    89101 gaaaaaatta attggcgtac ttgtcgtttt aggcatattt ctcggcggaa gttttgcagc
    89161 ttatcattat ttttacggcg gcgaagctta ttatacaaaa atcacgacga atggcgaaat
    89221 gtcagctacc caagcagata acggagagaa attcacgact tatacctaca agcaagcggc
    89281 ttataataaa gatggtgaaa aaaaacaggt gacgcttcgc gaagaaagag aacatccatt
    89341 aaagatgaat gcgtatctta aactcaaagt caatcctcgt aaaggtgtat taagctggga
    89401 agaaataaag gcttctgaag tgcctaaaaa tgcggcagaa aaaatcgata attagtaaat
    89461 ggtcttaaaa ttttattaga taaaa
//
LOCUS       DACJGB010000003        82992 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.003, whole genome shotgun sequence.
ACCESSION   DACJGB010000003 DACJGB010000000
VERSION     DACJGB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.003"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..562)
                     /locus_tag="IYI34_000219"
     CDS             complement(<1..562)
                     /locus_tag="IYI34_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAR0558124.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(749..1399)
                     /locus_tag="IYI34_000220"
     CDS             complement(749..1399)
                     /locus_tag="IYI34_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558125.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="IYI34_000221"
     CDS             complement(1942..2877)
                     /gene="whiA"
                     /locus_tag="IYI34_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAR0558126.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2911..3909)
                     /locus_tag="IYI34_000222"
     CDS             complement(2911..3909)
                     /locus_tag="IYI34_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAR0558127.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="IYI34_000223"
     CDS             complement(3906..4790)
                     /gene="rapZ"
                     /locus_tag="IYI34_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAR0558128.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4925..5656)
                     /locus_tag="IYI34_000224"
     CDS             complement(4925..5656)
                     /locus_tag="IYI34_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAR0558129.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5660..6925)
                     /locus_tag="IYI34_000225"
     CDS             complement(5660..6925)
                     /locus_tag="IYI34_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAR0558130.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="IYI34_000226"
     CDS             complement(7188..10004)
                     /gene="uvrA"
                     /locus_tag="IYI34_000226"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAR0558131.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="IYI34_000227"
     CDS             complement(10014..12008)
                     /gene="uvrB"
                     /locus_tag="IYI34_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAR0558132.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12267..13769
                     /locus_tag="IYI34_000228"
     CDS             12267..13769
                     /locus_tag="IYI34_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR0558133.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13771..14508
                     /locus_tag="IYI34_000229"
     CDS             13771..14508
                     /locus_tag="IYI34_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR0558134.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14553..14726)
                     /locus_tag="IYI34_000230"
     CDS             complement(14553..14726)
                     /locus_tag="IYI34_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAR0558135.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14702..15367
                     /locus_tag="IYI34_000231"
     CDS             14702..15367
                     /locus_tag="IYI34_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAR0558136.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15449..16615)
                     /gene="dnaJ"
                     /locus_tag="IYI34_000232"
     CDS             complement(15449..16615)
                     /gene="dnaJ"
                     /locus_tag="IYI34_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAR0558137.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16766..18595)
                     /gene="dnaK"
                     /locus_tag="IYI34_000233"
     CDS             complement(16766..18595)
                     /gene="dnaK"
                     /locus_tag="IYI34_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAR0558138.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18646..19209)
                     /gene="grpE"
                     /locus_tag="IYI34_000234"
     CDS             complement(18646..19209)
                     /gene="grpE"
                     /locus_tag="IYI34_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAR0558139.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19303..20346)
                     /gene="hrcA"
                     /locus_tag="IYI34_000235"
     CDS             complement(19303..20346)
                     /gene="hrcA"
                     /locus_tag="IYI34_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAR0558140.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20580..21746)
                     /locus_tag="IYI34_000236"
     CDS             complement(20580..21746)
                     /locus_tag="IYI34_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAR0558141.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21762..21989)
                     /locus_tag="IYI34_000237"
     CDS             complement(21762..21989)
                     /locus_tag="IYI34_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAR0558142.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22473..23063
                     /locus_tag="IYI34_000238"
     CDS             22473..23063
                     /locus_tag="IYI34_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAR0558143.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23253..23966
                     /locus_tag="IYI34_000239"
     CDS             23253..23966
                     /locus_tag="IYI34_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558144.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24081..25091)
                     /locus_tag="IYI34_000240"
     CDS             complement(24081..25091)
                     /locus_tag="IYI34_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAR0558145.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25128..25610)
                     /locus_tag="IYI34_000241"
     CDS             complement(25128..25610)
                     /locus_tag="IYI34_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAR0558146.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25760..26128
                     /locus_tag="IYI34_000242"
     CDS             25760..26128
                     /locus_tag="IYI34_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAR0558147.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26593..27045
                     /locus_tag="IYI34_000243"
     CDS             26593..27045
                     /locus_tag="IYI34_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558148.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27169..28020)
                     /locus_tag="IYI34_000244"
     CDS             complement(27169..28020)
                     /locus_tag="IYI34_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAR0558149.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28033..28818)
                     /locus_tag="IYI34_000245"
     CDS             complement(28033..28818)
                     /locus_tag="IYI34_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0558150.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29170..30111)
                     /gene="ribF"
                     /locus_tag="IYI34_000246"
     CDS             complement(29170..30111)
                     /gene="ribF"
                     /locus_tag="IYI34_000246"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAR0558151.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30115..31038)
                     /gene="truB"
                     /locus_tag="IYI34_000247"
     CDS             complement(30115..31038)
                     /gene="truB"
                     /locus_tag="IYI34_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAR0558152.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31406..33768)
                     /locus_tag="IYI34_000248"
                     /pseudo
     CDS             complement(31406..33768)
                     /locus_tag="IYI34_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34216..34389
                     /locus_tag="IYI34_000249"
     CDS             34216..34389
                     /locus_tag="IYI34_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0558153.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34546..34893)
                     /gene="rbfA"
                     /locus_tag="IYI34_000250"
     CDS             complement(34546..34893)
                     /gene="rbfA"
                     /locus_tag="IYI34_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAR0558154.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34920..37226)
                     /gene="infB"
                     /locus_tag="IYI34_000251"
     CDS             complement(34920..37226)
                     /gene="infB"
                     /locus_tag="IYI34_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAR0558155.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37239..37550)
                     /locus_tag="IYI34_000252"
     CDS             complement(37239..37550)
                     /locus_tag="IYI34_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAR0558156.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37547..37840)
                     /locus_tag="IYI34_000253"
     CDS             complement(37547..37840)
                     /locus_tag="IYI34_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAR0558157.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37862..39037)
                     /gene="nusA"
                     /locus_tag="IYI34_000254"
     CDS             complement(37862..39037)
                     /gene="nusA"
                     /locus_tag="IYI34_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAR0558158.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39060..39533)
                     /gene="rimP"
                     /locus_tag="IYI34_000255"
     CDS             complement(39060..39533)
                     /gene="rimP"
                     /locus_tag="IYI34_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAR0558159.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39678..44036)
                     /locus_tag="IYI34_000256"
     CDS             complement(39678..44036)
                     /locus_tag="IYI34_000256"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAR0558160.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44248..45957)
                     /locus_tag="IYI34_000257"
     CDS             complement(44248..45957)
                     /locus_tag="IYI34_000257"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAR0558161.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46025..47293)
                     /gene="rseP"
                     /locus_tag="IYI34_000258"
     CDS             complement(46025..47293)
                     /gene="rseP"
                     /locus_tag="IYI34_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAR0558162.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47454..48254)
                     /locus_tag="IYI34_000259"
     CDS             complement(47454..48254)
                     /locus_tag="IYI34_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAR0558163.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48251..49063)
                     /locus_tag="IYI34_000260"
     CDS             complement(48251..49063)
                     /locus_tag="IYI34_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAR0558164.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49258..49815)
                     /gene="frr"
                     /locus_tag="IYI34_000261"
     CDS             complement(49258..49815)
                     /gene="frr"
                     /locus_tag="IYI34_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAR0558165.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49818..50540)
                     /locus_tag="IYI34_000262"
     CDS             complement(49818..50540)
                     /locus_tag="IYI34_000262"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAR0558166.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50676..51557)
                     /locus_tag="IYI34_000263"
     CDS             complement(50676..51557)
                     /locus_tag="IYI34_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAR0558167.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51656..52438)
                     /gene="rpsB"
                     /locus_tag="IYI34_000264"
     CDS             complement(51656..52438)
                     /gene="rpsB"
                     /locus_tag="IYI34_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAR0558168.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52797..53276
                     /locus_tag="IYI34_000265"
     CDS             52797..53276
                     /locus_tag="IYI34_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAR0558169.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53493..54584
                     /gene="phnW"
                     /locus_tag="IYI34_000266"
     CDS             53493..54584
                     /gene="phnW"
                     /locus_tag="IYI34_000266"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAR0558170.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54577..54705
                     /locus_tag="IYI34_000267"
     CDS             54577..54705
                     /locus_tag="IYI34_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAR0558171.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54709..55254
                     /locus_tag="IYI34_000268"
     CDS             54709..55254
                     /locus_tag="IYI34_000268"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAR0558172.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55708..57399)
                     /locus_tag="IYI34_000269"
     CDS             complement(55708..57399)
                     /locus_tag="IYI34_000269"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAR0558173.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57819..58766)
                     /gene="arcC"
                     /locus_tag="IYI34_000270"
     CDS             complement(57819..58766)
                     /gene="arcC"
                     /locus_tag="IYI34_000270"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAR0558174.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58881..59900)
                     /gene="argF"
                     /locus_tag="IYI34_000271"
     CDS             complement(58881..59900)
                     /gene="argF"
                     /locus_tag="IYI34_000271"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAR0558175.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59991..61220)
                     /gene="arcA"
                     /locus_tag="IYI34_000272"
     CDS             complement(59991..61220)
                     /gene="arcA"
                     /locus_tag="IYI34_000272"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAR0558176.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61681..62382
                     /locus_tag="IYI34_000273"
     CDS             61681..62382
                     /locus_tag="IYI34_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAR0558177.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62991..64007)
                     /gene="tsaD"
                     /locus_tag="IYI34_000274"
     CDS             complement(62991..64007)
                     /gene="tsaD"
                     /locus_tag="IYI34_000274"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAR0558178.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(64004..64468)
                     /gene="rimI"
                     /locus_tag="IYI34_000275"
     CDS             complement(64004..64468)
                     /gene="rimI"
                     /locus_tag="IYI34_000275"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAR0558179.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64475..65017)
                     /gene="rimI"
                     /locus_tag="IYI34_000276"
     CDS             complement(64475..65017)
                     /gene="rimI"
                     /locus_tag="IYI34_000276"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAR0558180.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(65001..65825)
                     /gene="tsaB"
                     /locus_tag="IYI34_000277"
     CDS             complement(65001..65825)
                     /gene="tsaB"
                     /locus_tag="IYI34_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAR0558181.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(65914..66894)
                     /locus_tag="IYI34_000278"
     CDS             complement(65914..66894)
                     /locus_tag="IYI34_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR0558182.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66918..68402)
                     /gene="galT"
                     /locus_tag="IYI34_000279"
     CDS             complement(66918..68402)
                     /gene="galT"
                     /locus_tag="IYI34_000279"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAR0558183.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68414..69403)
                     /gene="galE"
                     /locus_tag="IYI34_000280"
     CDS             complement(68414..69403)
                     /gene="galE"
                     /locus_tag="IYI34_000280"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAR0558184.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69651..69818
                     /locus_tag="IYI34_000281"
     CDS             69651..69818
                     /locus_tag="IYI34_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAR0558185.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69880..71691)
                     /locus_tag="IYI34_000282"
     CDS             complement(69880..71691)
                     /locus_tag="IYI34_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAR0558186.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71688..72053)
                     /locus_tag="IYI34_000283"
     CDS             complement(71688..72053)
                     /locus_tag="IYI34_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAR0558187.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72216..72611)
                     /locus_tag="IYI34_000284"
     CDS             complement(72216..72611)
                     /locus_tag="IYI34_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAR0558188.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72629..73591)
                     /locus_tag="IYI34_000285"
     CDS             complement(72629..73591)
                     /locus_tag="IYI34_000285"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAR0558189.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73591..73803)
                     /locus_tag="IYI34_000286"
     CDS             complement(73591..73803)
                     /locus_tag="IYI34_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAR0558190.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73824..74522)
                     /locus_tag="IYI34_000287"
     CDS             complement(73824..74522)
                     /locus_tag="IYI34_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAR0558191.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74542..75084)
                     /locus_tag="IYI34_000288"
     CDS             complement(74542..75084)
                     /locus_tag="IYI34_000288"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAR0558192.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75216..76220)
                     /locus_tag="IYI34_000289"
     CDS             complement(75216..76220)
                     /locus_tag="IYI34_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAR0558193.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76217..77206)
                     /locus_tag="IYI34_000290"
     CDS             complement(76217..77206)
                     /locus_tag="IYI34_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAR0558194.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77203..78009)
                     /locus_tag="IYI34_000291"
     CDS             complement(77203..78009)
                     /locus_tag="IYI34_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558195.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78175..79131
                     /locus_tag="IYI34_000292"
     CDS             78175..79131
                     /locus_tag="IYI34_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0558196.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79208..79726)
                     /locus_tag="IYI34_000293"
     CDS             complement(79208..79726)
                     /locus_tag="IYI34_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR0558197.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79814..79963)
                     /gene="rpmG"
                     /locus_tag="IYI34_000294"
     CDS             complement(79814..79963)
                     /gene="rpmG"
                     /locus_tag="IYI34_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAR0558198.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80191..80637
                     /locus_tag="IYI34_000295"
     CDS             80191..80637
                     /locus_tag="IYI34_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAR0558199.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80832..82727)
                     /gene="asnB"
                     /locus_tag="IYI34_000296"
     CDS             complement(80832..82727)
                     /gene="asnB"
                     /locus_tag="IYI34_000296"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAR0558200.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23579 a        17672 c        14333 g        27408 t
ORIGIN      
        1 ctataaacat caccccaaat aaaacagaaa acagtataag cattataaaa attatcaaca
       61 accttgatcc ttcttttata gattcaggaa acagtataat tttagtacta gataaattgt
      121 ttacaccgat tagtgttaaa atatctttat tcttcttgct aacataaccc tttatgcaca
      181 aaagaacagc tatacttaaa gtaacaaata aataacgtat tccaatagaa aaatcactat
      241 aaatactctc tagcatagga ccgacaattg aagaagtcac tactgcaaat gctggaccta
      301 tccaagcaac attagaatgt tctttctctc ttttcctttg taaatctttg ttgagtatta
      361 atctatttat ttcttctgca ggcaagttaa ttttcataac cttatggggg attatccaat
      421 taaaaatcgg aaaaaggtag cctccccaaa aagtatagtg atctgtgtca atcagataat
      481 aacctgattt tgattccaga ataacatatc tcaaagccga acgaaaattt ttcatgaata
      541 tccataatct cactgtttcc acacctaatt cctccaattc caattaaatg gatttatagc
      601 atcaccaatt atgttaactg cctgaccaac ctcattgata aaattggcac cactatccca
      661 tgcttcaaca atgctatata tctatcaaac aaaaaatgga aaactaaata ctcgcgcaca
      721 taaattttac attctgtttt ttataagact aataataatc attgaaaaaa tttcttatcg
      781 gtggcgcata ttcccaattg attaatgtga tcccatattc tgcaagtatt ttacgagctt
      841 cttctaagtc atagtgagaa aaatgcataa aacctgcaat acgatcacgg actgtgagct
      901 gtggcttgct tccaccatca ttcataaaat atccagcaga gccactactt aaagtgattg
      961 ttacatcttc actttctatt attaagaatt cataatgaaa tgctttcaac tctccactat
     1021 tatttaaaag ttttatactc caacttcgtc taggttgagt tgtcacattc cctctaaaac
     1081 ttcctataaa ttctgcaatc accgtatctt caatcaaaat tctttcctca tctatttcat
     1141 attcaagact aaacggaaat tctccatatg taatctctgg caatggtggt gctgtagttc
     1201 tccaaataaa tacaataact gtgaatagaa aaaatccaac gatcgatata aatgttatga
     1261 gacctgctcg aagtaaaacc cgatataata aaaatgctgt atttcgcttt gctatatcta
     1321 gaaaaataaa aagtactgca atcataatga tagtaaaagt tgtaaaaaag cacatttttt
     1381 gccctccctt tataaacatc gattctcaaa ttctaaacca aatcttgata gttcaagttc
     1441 aggtataacg tataccaacc atacatccca attactattt atgaacttat gattatcact
     1501 gaataaatca ctccatcccc ttgttaaaac ttctacatta attacacctg gttgttttcg
     1561 actagctaat ccaatgaact tatcaaccat ccttatccac caaccacgag aatcttgacc
     1621 agaagtaaga aaaacagttc tcaaggttga tcgagcagtt ccttccagtt tttaattaat
     1681 ctccagttat agataaatac ttgatcctat ctatatcagg acttctatat taagcaaaag
     1741 tttatgtata aaactaaaat cttcattcag ttgtctcccc gctttttata cgattgtatt
     1801 aaaaaacaat agaagccttt caacattatg ttactccagt aaagttggaa ttatagtttg
     1861 acggaagaaa agcaagtata gcttttaaaa aaaactgata cattcgcatc tcctgcttca
     1921 tgtatcagtt ttttctttgg attaaactgc ttcttcacgc agtttttccg caaattcatt
     1981 tattttgcga attcggtgat taattcccga ttttgatatt gcacctgagg gaatcatttc
     2041 ccctaattct ttcaagctga cctctggatg ttgaagccga agttctgcga tttcctgaag
     2101 tttttcagga agggcatgta gaccgacctt tgcttcgatc aattcgatgt tttcgatttg
     2161 tttagaagcc gcatcaatcg tcttgttcag atttgctgtc tcacaattga ctagacgatt
     2221 gaccgaattt ctcatatccc gtacgattcg aacatcttca aatcggagca ttgagttggt
     2281 tgcaccaatc aatgttaaga aatcagcaat tttttctgct ccttttaaat acgaaatata
     2341 gccattgcgc cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca tatcgcaaat
     2401 gtcttggtta tgctgttcat aaatagaaaa tatttccaaa tgataacgac tcgtttccgg
     2461 attattgact gaacctgagg ccataaaagc accacgtaag taagaacgca ttttttgcga
     2521 atttcccata atctcgtccg aaacgtgtcc attgaacatc ataccatcca tgatatcaag
     2581 atccatcaac acatcttttg tgccttgctt caaccggacg atatagacat tgttcttttt
     2641 taatttcatt tttttacgaa cgagtagctc acttcgtacc tgataatgat ctttcaaaag
     2701 agaatagata cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa taaactgctg
     2761 gttcacaata cctaaagagc cgttcattcg cagcaaagca gctaattccg ctttcgcatg
     2821 ttctcgatga acttccaaac cagttagttc tttcttcaca tctgcagcaa atgacatcct
     2881 attctcctcc tttctccaac aataaattgc ttaatatttt gaaccgaaaa ctaaacggaa
     2941 caattcttct actactttgt ctccatcgtg gaagacacca ccatctttca attctaaaaa
     3001 gtcggtggaa atcactcgac atccttcatt tctcaatccg ttgaagtcgt gtgcgacttg
     3061 tactagatac tcatcataga tctctgggtc catataatct tcaggcactt tttccgtatt
     3121 gaccaatact gtatcgataa aaggctgatt caaatgcttg tgtaaaacac gtatatgatc
     3181 ggcatctgtg aaatgttccg tttccccttt ttgggtcatg atattacaga tatagacggt
     3241 ttcagcagtt gtcttgagca ttgcttcgcc gatttctggt atcactaaat ttggaaggat
     3301 actggtgaat aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa ttgctgaaac
     3361 gacttttcga gccgctttgg cttcactttt atcatttgtg ttgcagacat acacacggtc
     3421 aatggtttta cggttgatcg cgattttaga ttctcctact gcagtcgttc cgtctttaaa
     3481 aacagcatgc aaggtcaaag gagtttctga agaaggataa acatgtccat ctacatgcat
     3541 catttttgct aataattgaa tcgcctcata agtgcttccc cgcatctcgg aaactgcggc
     3601 aatgatcaga ttaccgatcg tatgattggc taaaaactga tcttcctttt tgaaacggta
     3661 ttggaaaata tcttcataga attgtggcat atccgataac gccactaaga catttctcaa
     3721 gtcaccagga ggagccatag cgatcgattc tctgattgca ccgctgcttc caccatcatc
     3781 tgcaacagtc actactgctg tgatatctgc tccttgattt cgtaaacttt tcaggataac
     3841 tggcagacca gtgccaccgc cgaccactac gatctttggt tttcttatcc ggtaggtttt
     3901 cattttcacg accggttcac cgtctctttc cttttatcct tgtcccgatg tgtcacattg
     3961 actttatagt ttttccctaa agcatttgcc aaacgttcag tcaatgccac agaacggtgt
     4021 tgaccgcctg tacagccgat tgcaactgtc aagctgcttt ttccttcttt aatgtatcca
     4081 ggcaatacag tttccagaag atccaaaaat ttatggtaga atgattgcgt ttcatcaaag
     4141 gacatcacat aatcatagac tggctgatct tttccggtca atggtcttag ttccgggata
     4201 taatgagggt tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat cggcaacccg
     4261 tatttaaaac caaatgatac aaattcgatc cgaaatcctt ggtctccctt tgatttgaat
     4321 gactcattga ttttttcacg taattgtcgt ggtgtcaatg ttgtcgtatc aatgatcacg
     4381 gaagcttggg ctttcaagtc gtctagtatc gcccgttctt tacgtatccc ttcagtgatc
     4441 aaaccgtcca tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc cactagttct
     4501 tcgtctgaag catctaaaaa taagatagaa gtgtcgatga agttcgtatt ttcgatttcg
     4561 atcagcatgt tttggatttc ttcaaagaag gaacgagaac gcaaatccac tcctaaagcc
     4621 atttttgtga ctttacctga ttctttgatc aattcccaga attttggaat caatcgaggc
     4681 ggcatattat cgatacaaaa atagcccata tcttcaaaac tttggatagc tactgttttc
     4741 cctgcaccac tcatacctgt tatgatgact aattgcaaat tatctgtcat atttacgaac
     4801 ccctctcaca ttgtgcataa taaaattata tcatcccggg cgtatcatta ccagttttct
     4861 ttttctttta caaaaagatt tggaccaaat atcttttagg atatttgccc caaatccaat
     4921 ttttttactt ttccgatgct gtatccaatg cctcgcttct ttcagctaat gcacgttcga
     4981 tcgttcgatc cgctaaaata gattgagcat cgatcactgg gtaatgatta tcttcagcaa
     5041 attcatgcat caaagataat tctgtgcaac ctaggatgac tttttcacag tttaggcgct
     5101 ctaccgcttc ctctaagatt tcgtaataga gctcttgatt caaataatcc gattccttga
     5161 tctcatgata gatcaggtaa ttgatttttt cttgaagcgc agtatcagga atcactgttt
     5221 cgaaaccaag atttttgact tcgcgttcat agattccagc tttcatactt ccttctgttc
     5281 ctagaatcgc tactctccct gtcgtatgct gacggacaag ttcatttgct gcttctcttg
     5341 gcatatgcaa gataggaata tccgttgccg cttgtaactc ttcaaaaaag tagtgtgcgg
     5401 tgttacaagt caacacaatg aagtttggtc gcaataaatt ttgtttctca atatcatcca
     5461 gcagaaacgg cattggattt tcttctgatc gatctaaaat ataagctgta cgatcaggaa
     5521 ctgttgcatg attgaataag acataattca aatattcttg atcttttgta gctttcgttc
     5581 ggtggttgat caagcgaaca aaactttctg tcgccatcgt gcccattccg cctaaaatac
     5641 tgaaaaaatt ctccatgaac tagccttctt tcacatgaaa atactttttg aatcttggaa
     5701 tatagttatg gaacatatat ttcatcaaca accagcgctt gattgatcgg tctttttcat
     5761 aaaatacagt agtaccgtaa cgattttctt tgatcatttg ctcagctagt tttttgtctt
     5821 cattttcccg agcatattct aaaaagattt ttttgggcac acccatccat aatcttgctt
     5881 tatctgattg attcgttcca taggtcgttt caacaaaggg tttgttgaac actcgatctt
     5941 ctgtgacaaa acgagccaga ttcaagccgt tcaatgtcac aaagaaactg ctacgacctt
     6001 gtctcaaatt gatttcaaat aatttatatt ctccatctct aggatcatac ttcatatcga
     6061 aattggcaaa gcctgtatat tcgatttttt ccaaaaatgc cttaatggtt tgatagattt
     6121 tttcgttata atctggcatg atcacaacgt aattaccaat cgatgctggt gtaggatctt
     6181 ctaatagtgg atgaccaagg cacatcatcc gaacttgatg atcttcatct acatacgcat
     6241 ttagcacacg catattacta tcatcacccg ggataaaatc ttgtacaatc atttcacttt
     6301 tataaccggc ttcgtatatc cggcctaaaa ttaaatcgaa ctcttctcta gtctctaaaa
     6361 taaaagcttt ttttcttcct tcaaattgga cagaaagata ctccacactg tttgcaggtt
     6421 tcaaagcaac tgggaattca aaaggcaatt cctgttccaa atgtccattt actaacattt
     6481 cttctctgac gatcaatgtt ttaggatacg gaagatcata ttcttcacaa acttcataaa
     6541 agcttacttt attgatcagc ctttcaaata aagaataatc aatatatgga cagataaaat
     6601 attctgacag ctcttgttta tgttgggaga tcaattctgc atatccatca ccacaagcaa
     6661 tcaaaagata ttttttactc ttatcagaat atctttcttt tgccaaacga agcatcgtct
     6721 cgataaatac agggtcttta tcaaatcctg gaataacttc tacattgaca attttactgt
     6781 agcgggtagg ggctaactga tccgaagcat atgcttggca gattttgccg tatgcttcat
     6841 taaaagaacg tgccatacca taaacgttca tgtcgcttcc taataatata gggataaatt
     6901 cttcattttc aatactgttc atcattatct ctcacttact cataatctct tgtttttctt
     6961 ttccaactac acaaggaata tcataccata aagaaaaaag caatcaaagg aaagtcctcc
     7021 gattgctcaa ttcttttttg aacggattga acttatagga agtttttatc tataccaatc
     7081 agtgtagaaa gaactaccat gtaatgattc tagaacactg atattaataa aacaagcaaa
     7141 aattcatttt tttatgtttt tttaactatc tcagctatct atcctcttta taccattatt
     7201 tttttcaaat agtgacctgt ataactttct tttacgttaa tgatttcttc tggtgttcca
     7261 gtcgcaagga tcgttcctcc accttctcca ccttctggtc ctaaatcaat gatataatct
     7321 gctgttttga ttacatctag attgtgttca atcactaaaa ctgtgttacc agcatctact
     7381 aatctttgta atacatgcaa caagcgggcg atgtcatcgc tatgaagtcc tgtcgttggt
     7441 tcatctagta tatagaaatt ctttccatta gagattttgt gcaattcact ggcaagtttc
     7501 atccgttgtg cctcaccacc ggacaatgtc gttgctggtt gccccatcgt cacataacct
     7561 aagccaacat caacaatcgt ttgcagtttg cgatgaatct ttggaatgtg cttgaagaat
     7621 tctacagcat cctctactgt catttccaaa atatcagcaa tgctttttcc tttgtaatgc
     7681 acttctaatg tttcagaatt atatcgttta ccatgacata cttcacaagg aacatagaca
     7741 tcaggcaaaa agtgcatttc gatcttaata attccatccc cgcgacaagc ttcacaacga
     7801 ccgcctttta cgttgaaact aaaacgccct ttcttataac cccgcatttt tgcctcgttc
     7861 gtttgagcaa ataacccacg gatatcatca aatacacttg tataagtcgc tggattactt
     7921 ctcggcgtcc ggccgattgg gctttgatcg atatcgatga tcttttcgat actttcgtag
     7981 ccggaaattg tcttgaattt acctggctta gcagaattct tatttaattt ttgcgctaac
     8041 gatttcttta agatactatt gaccaatgta ctctttccag aaccagacac acctgtgact
     8101 gctacaaatt ctcctaatgg aaattcaaca ctaatatttt tcagattgtt ttctgaagca
     8161 ccagtgatct tgattgcttt cccattacct ttacggcgtt cttttggtac agggattgat
     8221 tttttccctg aaagatattt ccctgtcaaa gaattttcat cattagctac ttcttcagga
     8281 gtgcctgcag ccacgatttg tccgccttgt tctccggccc caggtccgac gtcaatcaaa
     8341 taatctgctg cccacatcgt atcttcatca tgttcgacta caacgagggt gttgccaaga
     8401 tcacgcattt tctttaatga gccaatcagt cgatcgttgt cgcgttgatg caatccgatc
     8461 gatggttcat ctaaaatata caaaactcca gaaagattcg atccaatttg tgtagccaat
     8521 ctgattcgct gcgcttctcc cccagataat gttccggaag cgcgactcaa agttagatag
     8581 tctagtccca cattttccaa gaatgaaagt ctgtcattta cttcttttaa aatcggtttg
     8641 gcaattactt tctcttgttc agataacgtt aagtttgaga agaaatctac tgctttttga
     8701 atagacaact catttgtttg cccgatattc gttccattga tttgaacaga tagagcttgg
     8761 ggattcaatc gataaccttt acatgtctga caagttaatt ctgtcatata aagacgcatt
     8821 tgctcacgcg tgaaatcact gttcgtatca tgataacgac gctcgatatt cttcaatacc
     8881 ccttcgaaag tcgtttctac atcgcgaacg ccaccaaaat cattttcata atggaaatgg
     8941 aaaagatcat ctgtcccatt caagatgatc tcctgatgct cttccggtaa ttcttcgaat
     9001 ggtgtatcta aatcgatacc gaaactttca caagcctgcg ctaacatttg aggataatac
     9061 tgagagctga ttggattcca tggaacgata gcaccttgac taagtgtctt cgttctatct
     9121 ggaatcacta aatcttgatc gacttctaat ttgacaccta aaccatcaca gtctggacaa
     9181 gctccaaaag gcgcattgaa tgagaacagt cttggttcta gttcgccaac agtaaatccg
     9241 caataaggac aggcatagtg ttcgctaaac aacatttctt cttcaccgat cacgtcaacc
     9301 agagcatagc cgtctgctaa tcgcagggcc gcctctaaag aatcaaatag gcgagaacga
     9361 atcccttctt tcacaatgat ccggtcgatg acgatcgcga tatcatgttt tttgtttttc
     9421 tccagctcag gcacttcact taaatcatag atctcaccat cgactcgaac gcggacgtat
     9481 ccctcttttt ggattcgttc gaatattttt ttatgttgtc cttttttctt taccacgatc
     9541 ggtgccaaca cttggatctt tgtgcgctca ggcaaagcca ttacttgatc tgccatttga
     9601 tctactgatt gactggtaat ctcgatatga tcgttgggac aaatcggatg tcctacacgt
     9661 gcaaacaaca agcgtaaata gtcattgatt tccgtcacag tacctactgt cgaccgagga
     9721 tttttacttg ttgttttctg atcaatagaa atcgccggac tcaatccgtc gatgctatct
     9781 acgtccggct tatccatttg tcctaaaaac tgacgagcat aagcagaaag gctctctacg
     9841 tatctccgct gcccttcagc gtacagcgta tcaaatgcta gggaactttt tccagaaccg
     9901 gacaaacctg taacgaccac gaatttatct cgtggaatcg ttacatctat atttttcaag
     9961 ttatgggcac gtgcaccatg aatcgtaatt ttatcgttag ccatgttgtc ctcctacttt
    10021 gaagctttta attctaaaat catatctcgc acattcgcgg ctgtttcaaa atccaaagct
    10081 tttgccgcat ctttcatttc tttttctaat ttcatcaata acgtgtcttt ttcttccttc
    10141 gtcatttctt cgtacgagac ttgtaccgct tcttttgtat catcttccga ttctttcgta
    10201 atagaaatca aatctcggat ttctttaatg atcgtttttg gaacgatccc atgttcttca
    10261 ttgtacttct gctgaatcgt acgacgtctt gacgtttcat ccatcgctag acgcatagaa
    10321 tctgttactt tgtctgcgta catgattact ttaccttctt cattccgagc agctcgtcca
    10381 atcgtttgga ctaaggaacg ttcactacgt aaaaagcctt ctttatccgc atccaagata
    10441 gcaacaaggg aaacttcagg tacgtctaat ccttcacgaa gcaaattgat tccaactaac
    10501 acatcaaatt cacctaatcg aagatctcga atgatttctg ttcgttccag cgttttgata
    10561 tcactatgca aatatttgac tttgatccct aattctttga aataatctgt gaggtcttct
    10621 gccatttttt tcgtcaatgt cgttacaaac acccgctggt ctttttcgac tcgttcattt
    10681 atttcgccta ctagatcatc gatttgtccc atgattggac gaacttcgat cactgggtca
    10741 agcagccctg tcggacggat gatttgttgg ataacggtat ctgtttgttc atgttcatat
    10801 ggtcctggcg tcgcagagac ataaatgatc tgattgacat gtttttcaaa ctcttctaac
    10861 cgaagtggcc gattgtctaa agcagatggc aaacggaaac cgtaatctac tagcatctgt
    10921 tttctcgctc ggtcaccatt atacattcct ctgatttgag gcatggttac gtgcgattcg
    10981 tccgccacga tcaaaaaatc ttcaggaaag aaatccaata gcgtataagg cggttcacct
    11041 tctttacgtc cgtccatatg ccgagaataa ttttcaattc ccgacgtgta gcccatttct
    11101 aacatcattt caatatcgta attggttcgc tgttctaaac gttgagcttc cagaagtttg
    11161 ttttcatttc gcaagacagt taaacgctgt tctaattccg ctttgatatt cgctacggca
    11221 tgctccatat gttcatcatt cgtcacaaag tgagtcgccg ggaagattgc tacgtgttcc
    11281 gtttctccta aaacttcacc tgtcagtgca ttgacttctc ggatacgttc gacctcatct
    11341 ccaaaaaact ccacacgtaa ggcacgttca tctcttgaag ccggaaaaat ctccacgaca
    11401 tcaccgcgca ctcgaaaacg accgcgctga aaatctatat catttctttc gaattggata
    11461 ctgactaagt cacgaatcaa ctgattgcga tctagttctg ctccctgtcg aatcgaaact
    11521 acttgttttt ggtactcaaa aggcgagcct aatccaaaaa tacatgaaac ggatgcaatg
    11581 acgatcacat catttctttc taataatgaa ctagtagccg aatgtcgtaa tttgtcgatc
    11641 tcatcattta cacttgaatc tttttcgata tacgtatcgc ttgaaggcac ataagcttca
    11701 ggctgataat aatcataata agaaacaaaa tattcaacag cattgttagg gaaaaattcc
    11761 ttgaattccc cgtacagctg accagccaat gttttgttat gggcaataat cagtgttggt
    11821 ttattcactt tctcaataag attggaaatc gtatatgttt tccctgttcc agtcgcacct
    11881 aataagattt gtgctttttt cccgccgact acgccatcca ctaattggtt aatcgcttca
    11941 ggctgatcac cagccggttg gtatttcgag acaagctcaa agtgtcggga ggtatctctc
    12001 tcaatcattc ttcgtcctcc ttttcattca ttccttttca ttttacgacc aataaaaaga
    12061 aaaggaaagc aaaggcattc cttactaaaa aattatacca caccgaacat actttcgcca
    12121 cttttttatc ttattataca tgttatgttt tataacataa aatgttagaa actcgcattt
    12181 ttttcatttt ttcgagctta cagtcttgtc tgattcagca cttttcgcct ataataataa
    12241 aatattggaa atttaggagg ttgattatga aaagaaagca tttatttcac tcatttgttt
    12301 tcttaatgac aggacttgtg acacttgcgt ttggtcatgt tgcacaagct gacaacacta
    12361 caacggcaga gtcaagcaca agcgagacaa tgcaaaaaat agagcccaaa aaagacgtat
    12421 acgtaattgc cagtgactct gcttttgctc catttgaatt tcaaaacgca aagggcgagt
    12481 acgaaggcat cgatgtaaaa ttgatggaaa acatcgccaa actgcaagga tttaacatcc
    12541 gaatggacta ccgtggtttc tctgctgctt tgcaagcttt ggaatcagga caagcagacg
    12601 gaatgattgc cggaatgact gtaacagatg aacgaaagaa aagctatgat ttttctgatt
    12661 cttactttga tagcgggatc caattagctg tcaaaaaagg agatacttcg atcaagtctt
    12721 atgaagattt gaaaggaaaa acagtcggag caaaagttgg taccgaaagt gctgaatttt
    12781 taagcgacca tgaatctgaa tatggctatt ctatcaaata ttttgatgct gctgatcaat
    12841 tgtacgacgg gttaaaagtt ggcgcaatcg atgcaatgat ggatgattat ccggtaatcg
    12901 gttatgcgat caaacaagga caagaattgg aaacaccgat caaaaaagaa tctggcggaa
    12961 aatatgcatt tgccgttaaa aaaggacaaa atcctgagct tcttaatatg ttcaatgaag
    13021 gattgaaaga attaaaaaga acaggcgaat acgataagat tattaaagaa tatgtagcgg
    13081 atggttcaga aacttccagt gagtcttcca ctgctgatga atcaacattt cttggactta
    13141 tccaaaacaa ctatcaagca ttgttgaaag gactttggag aacaattctc ctaactttga
    13201 tctcctttgc tttggctata gtaatcggtg tgatttttgg attattcagt gtagcaccaa
    13261 tacgcgctct tcgcacaatt gctagtattt atgtcgatat catccgagga attccaatga
    13321 tggttcttgc gttctttatt ttctttgggt tgcctggtat cgttggattt acgattcctg
    13381 acttccttgc tggtgttatc accttgactc ttaacgcaag tgcttatatc gcagagattg
    13441 ttcgaggcgg gatcaacgcc gttcctgtcg gacaaatgga agcttccaga agtttaggtc
    13501 tttcttataa tcggacaatg caaaaaatca ttttgccgca agcgatacgc atcatgatcc
    13561 catcattcgt caatcagttc gttatctcat taaaagacac aacgatcatt tccgcaatcg
    13621 gtgtagtcga attgctgcaa actggtaaga tcattgttgc acgtacaaca caaagtacgt
    13681 atgtttactt gatcattgcg atcatgtact tgattttgat cacagcattg acaaaattag
    13741 caaaagtttt agaaaagaag gtgaagtaag atggcagaaa aaatcttagt tgagcattta
    13801 gtcaaaaaat acggcgataa cacagtatta aacgacatca atgtatccat caacgaagga
    13861 gatgtcgtct gtatcattgg cccttcaggt tcaggaaaaa gtacattctt gcgttgcttg
    13921 aatcgtttag aagaaccttc tgacggtgaa attattattg acggcgccca cttgatggat
    13981 aaaagcacag atatcaactt ggtgagacaa catatcggaa tggtattcca acactttaac
    14041 ctattccctc atctttctat tttggaaaac atcattttag caccgatgga cctgaagaaa
    14101 gaatctagag aagaagcaga gaaaaaagca atcaaattac tgcagacagt tggtctagaa
    14161 gataaaaaag atatgtatcc tgaaatgctt tctggtggac aaaaacagcg tgtggctatt
    14221 gctcgtgctc tagcgatgaa ccctgacatc atgctttttg atgagcctac ttctgcactc
    14281 gatcctgaaa tggtcggtga tgtcttgaac gtcatgaaaa agttagcaga acaaggtatg
    14341 acgatggtta tcgttacgca cgaaatgggg ttcgctaaag aagtggccaa tcgcgtcatg
    14401 ttcattgacg acggaaactt tttagaagat ggaacgcctc aacaaatctt tgaaaatccg
    14461 caaaatgaac ggacaaaaga tttcttgaat aaagtattaa atatttaata aaaaccaaaa
    14521 atgcacggtc gccatgaagg gaccgtgtat ttttatcttc ttattttctc acctaatcgt
    14581 atcagccgca tcgcttgttt tcctgcacaa acaagcaatc gctctacctg ttccatcgca
    14641 taagaaagta ccatcggttc attgatgata tcaagtacat agtcgatacc accctcactt
    14701 aattgagctt gatttttctt ctccatatta tgtagttgtg ggacaacttt ctgacaatca
    14761 gcaattgtgt caagaacgga tcaatccttt attggagttg ctcgaaaggg tcttctctta
    14821 ttacggacag gctttgctca ctgtacatag acaacaaatc attatcctag tcaatcggat
    14881 ttccagagcc tcattgctat cattgcttga taaaatccag acaaagttta ataaaatgta
    14941 tcagttacag ctaaactttg gaattgggtc gctttgttat acagagcaag agacaccgca
    15001 atctttcctt catgccaaac aagtttgcga atggatagct ttccatcaat ctgtgaacga
    15061 gatccgcttt tttgaagatt tagacctcgg gatcgtttta ccagcaatcc cgtcagatca
    15121 acgaacatta tatgtaaaac gtattttgaa aagcctgaca gaagaagaag tacatttgtt
    15181 caagaagact ttggcatgct tcagtaaaaa taacggaagc atcaaaaatt gctcagaaga
    15241 attatttatt cataaaaaca ccttgcaata tcgattgaac aagttccatt ctctcactgg
    15301 ttatacccct agaaactatg acgattatca tattctaaag ctggcttttt tgttggtaca
    15361 aacatgacaa ctacaaaaaa accaggcgaa catatgattg ttcgtctggt ttttaattat
    15421 taatgactta gcgtttttac tagaaaaact acttccgctt tttcccgcca aaagcatctt
    15481 taaacttatc gaagaaaccg tcttcttgct gttcttcaac tttgatgccg ccagcttcag
    15541 cgaatgcacg taatgcttct ctttgttgtt catttagatt tttcggtgtg atcagtttta
    15601 ctttgacatg ttggtcgcct gttgcaccac cacgtaactt cggagcgcct tttccacgta
    15661 aacggaagtt cgtacctgtc tgtgttccag cagggatctt caatttcaca tctccatgga
    15721 cagttggtac ttggacttca tcccccaaag ctgcttgtac aaaacttaaa ggcaattcat
    15781 agtaaatttc agaaccttca cgatcaaaga tatcactttc ttctacacgg aagaccacat
    15841 acaaatcacc aaatggtccg ccgttttctc cagcttctcc ttgaccagct aaacgcattt
    15901 gttgtccttc ttctacacca gctggtacat tcactttaac tgaatgtgct ttcttctcat
    15961 gtcctgagcc atgacaggtt ggacaaggtt ctttgatttc tttccctgtc ccatgacaaa
    16021 catcacaagt ttgacggctc atcactcgtc caagtggtgt ttggcgttct acattaatcg
    16081 tacctgcacc atgacattta tgacaagtag ttggttgtgt gcctggtttt gcaccgttcc
    16141 caccacaagt atgacagatt tcttcccgat tatacttgat ttctttttct acaccaaaaa
    16201 tcgcttcttc aaaagacaag tcgaccgtat attgcaaatc agcaccttga cgaggcgcat
    16261 ttggatcgac tgaccgaccg cctcctccaa agaaagagtc gaagatatct tcaaaaccac
    16321 cgaagccgcc accagagaag ccgccgaatc caccaaatcc gccaccagct ccaccagcac
    16381 cgtaatttgg atctgtgcct gcatgcccgt attggtcata tgcagcgcgt ttttgcgggt
    16441 cacttaaaat ctcatatgct tctgatactt ctttgaattt ttcttccgca tcaggttctt
    16501 tattgatgtc aggatggtat tttttcgaaa gcttacgata agctttcttt atttcatctt
    16561 ctgatgctcc ttttgagagc cctaagactt cataataatc acgttttgtt gccataggct
    16621 ttcctcctat tggttctcat cattttcacg tcgcttgtta tcatatcata ttttgtttga
    16681 aacaaaaacc gatacttcaa actatttttc aaaagaaaaa gccaaagtag caactttggc
    16741 cttctctcga aattcagtga actgattatt tatcgtcgcc atcgacttct tcaaaatcag
    16801 catcaacgac atcatctgct ccgccttgag ctgcttctgg gttttcttga gcttgttgtt
    16861 gtgccgcttg ttcgtatagt ttaacagtca agttttgaac gatttcgttc aatgcatcgc
    16921 gtttagtttt catttcttcg atgttgtttg cttcaacagc ggctttcaat tcatcgcgag
    16981 ctgtttctgc ttttttcact tcttcttcgt ccactttgcc ttctaattct ttcaatgttt
    17041 tgtccacaga gaatagcaat gcatctacat cattacgtag gtcaacttcc tctttacgag
    17101 ctttatctgc ttctgcattt gcttctgcat cttttaccat acgttcgatt tcttcgtctg
    17161 ttagacctga agaagatttg atcgtgattt tttgttcttt ttgtgtacct agatctttcg
    17221 cagatacatt tacaatcccg tttttatcaa tatcgaatgt tacttcgatt tgtggaatac
    17281 cacgaggagc tgctggaata tctgtcaatt ggaatcttcc tagagttttg ttgtcagcag
    17341 ccattgggcg ttcaccttgt aatacatgga tgtctactgc tggttgatta tctgccgcag
    17401 ttgagaatac ttgtgattta cttgttggaa tcgttgtatt gcggtcaatc aatttagtga
    17461 acactccacc cattgtttcg atacctaatg acaatggtgt aacgtcaagt aaaacaacat
    17521 ctttcacatc accagtgatc acgccacctt ggatagcagc acccatagca actacttcat
    17581 cagggttaac tgatttgttt ggttcttttc ctgtttcttt acgtactgct tctacaactg
    17641 cagggatacg agtagatcca ccgactaaaa taacttcgtc gatttctgat tgtgataaac
    17701 ctgcatcttt tagcgcttga cgtactggta cttttgtacg ttctactaag tcagctgtta
    17761 attcatcaaa ttttgcacgt gtcaatgtca tttccaaatg caatggtcca gattctcctg
    17821 ctgtgataaa tggcaagctg atttgtgtgc ttgatacgcc agataaatct tttttcgctt
    17881 tttcagcagc gtctttcaag cgttgtaaag ccattttgtc ttgagacaag tcaatgctgt
    17941 tttctttttt gaattctgct accatgtagt cgatgatttt gttatcaaag tcgtccccac
    18001 ctagatggtt gtcgccagct gttgacaata catcaaagac accgtctcct agttcaagga
    18061 tagatacgtc aaaagtaccg ccaccaaggt cgaatactaa gattttttcg tcgcgatctg
    18121 ttttatctaa accataggca agagcagctg cagttggttc attgacgata cgttctactt
    18181 ctagaccagc gatcttacca gcatcttttg ttgcttgacg ttgtgcatca ttaaagtaag
    18241 ctggcacagt gataactgct ttttcgactt tttcacctaa atagtcttct gcgaatcctt
    18301 tgatatattg aaggatcatt gctgagattt cttgtggtgt atatgattta ccttctacat
    18361 ctactttgta acctgcttca ccgatatggc gtttgattga tgaaatcgta tttgggtttg
    18421 tcacagcttg acgttttgca acttccccta cttggatttc tccatttttg aatgatacaa
    18481 cagaaggtgt tgtacggttt ccttctgggt tagcgatgat ttttgcttcg tttccttcca
    18541 atactgctac tgcagagtta gtcgtaccta agtcaatacc gataatttta ctcataatta
    18601 acgtctcctt ctttaattga aagtgtaagt tttatatccg cacaattatt gtgcaacaat
    18661 gaccatggtt ggtcttaaca cgcgatcatg taatttgtag ccttcttgta gtacttctac
    18721 aatcgtatcg gctggtgtat cttcagtcgc tggaacagtt tgtactgctt gatgaagatt
    18781 aggatcaaag gcttctcctt tagcagggat tttttcaatt ccttcttctt ccagtgcgtt
    18841 acgcaaactt tccaagacca tttccacgcc ttttttcaaa cctgcccctt gatcatctga
    18901 cacttctgtg gctaatgcac gttccaggtt gtcaattgat ggaagtaatt ttttggctaa
    18961 atcttgtgaa cggtattttt gtaattgctc acgctcattt tttcctcgat ttgccatatt
    19021 tgcgatttca gcacgagccc gcaagtattt atcttccatt tcttctaact cagcttttaa
    19081 tttcgctgtt tcgaattctt ccgcttcagc ttctgaaata cccgaatctt ctgcagcttc
    19141 gacatcgatt tccggttctg gctgagcatc tgtcatttct ttatctagtt cttcttgatt
    19201 ttctttcacg atacagtctt cctttcatta ttcagtcagc tgtttgattg ctcttactaa
    19261 gaaagagaga acgatcagct gcaaatagct tcgttattca cattacgagt ttgacaagtc
    19321 aagcgaacgg tagtaatcag caagtttagc tgctagttct cttcgataaa catcgactag
    19381 accgaacatc cgagaataag gcatactagt aggccctaac aaggctattg tacctcttcc
    19441 atgaccatct atctcgtaac ttgcttgaat catactcata ttctgcaata aatcattgcc
    19501 taattcagca ccaatcttga tatgaatatt gctatccatc ggagtgatca tttgtgtaag
    19561 tccgtctgag tctttcataa aagaaaacat cgatttgaac tgatcaagat cttgacgggg
    19621 ctcataatcc aagagattca tttgtccact gacataaacc ttatcttcaa aaacatgacc
    19681 aagaacggtg tcaaataact gagacatccc ttctgtcgtt tggaaatatt tatgaagaat
    19741 catcggaatc tccgtacgaa gtttatggta caccgtcatc agaggatctc cgactagacg
    19801 gtcgtttaca atctgtacca ttttttctag ttcctgactt cccatatatt cgggaagagt
    19861 gaacacttgg ctttctacat tccccatatc tgttacgacg atagcaataa tctgtcgatt
    19921 attcaagggt acgatacgaa agccagtcag tcggcggtct tttatttcag gtcccaatga
    19981 aaaagtcgta tagcttgtca gctcagataa tatctcagca gattgcttaa taatctcatt
    20041 gatttcatga aattctctgc ctagagactg acgaatcagc agcaaatcat cttctgctac
    20101 gcgagcaggt tttaacaaat gatccacgta ataacgatag cctaaagcag atggaatccg
    20161 cccagaagaa gagtgagtct tcaacagcaa tccttcatct tctaatgctt tcatatcatt
    20221 gcgaatcgtt gctgagctgg ccttgactcc ctcagacatc aatgttttgg aaccaacggg
    20281 cacgccacta ctcgtatagt gttggatgat cagacgtaaa atgtcgtttt gtcgctttgt
    20341 taacatcttc ctcacctctc attagcactc atatattcta agtgctaatc cacttataaa
    20401 tatagcaaga agcccttctc ctgtcaagag aaagactcca ttttttagca ctctaaacac
    20461 aagagtgcta attctaaata attgatgtaa atccagttat atcaacgttt caatagtcct
    20521 ttattgtatt tttgaacttt ttttatttaa attcagataa aaaacaaata ttctttaaac
    20581 tatttttcaa acaaaaagcg ctcaaacaca ttgttcccca aaaaggtccc tcttggtgtc
    20641 aaataaacac ggtcttcttc attaacaagt aacccttctt gctctaattc ccttagcaca
    20701 tcaccgtaca ccgattcaat tgttttttga tagcgatcat agaaatgttt tttagtaatc
    20761 cctgtttttt ttcttagtcc taagaacatt tcttcttcta tttgattttt tcgtgtcaat
    20821 ttttcttctt ccaataccgg gcgttcattt gctcgtaatg ggcgtaaata gtgttggatt
    20881 ggtcctttgt tgcggtaacg aatatctcca agataaccgc tagcaccagc tccaaaccca
    20941 aaatagtggg cattgttcca gtacatgagg ttatgttgag attcatgtcc ggagagccca
    21001 aagttactta tttcatattg gtgacgtcca tgcttttcca ttgcttcaat cgtctcttca
    21061 aacattctgg tttccgtttc ctgatcaggc agttcgagtc ttccttgtcg tacccagttc
    21121 atgaacattg ttttgttttc gaggatcaat gagtacaaag aatagtgagg cagatccaaa
    21181 gccaaagctt tttccaaagt atccagatat ccttctagtg tttgtcccgg taatgcataa
    21241 atcaaatcga tgcttacatt ggtaaaattt tctgcttcta aaaattgcat agtttgatac
    21301 acatcttctg cggtgtgttt acgtccaatt tttttcaata agcgattatc aaatgtctgc
    21361 actcccatgg ataaacgatt gacgccatag tttttcatta cttgaagttt ttcacgggtc
    21421 aaatcacctg gattcgcttc tacagtaaac tcattaccat tttgaaaagg caggatctct
    21481 ctagcacctg ataacagtcg atcaagttgt ttagcagata aagaagttgg tgtcccgccc
    21541 cctacgtaaa gtgtttctaa attcgctgtc ggatgacttt ctagcattat tttcatttca
    21601 ttcagcagca tttccacata ctcatctact ggctgccctt ctaaaaaaac tttattaaaa
    21661 tcacagtaat agcagatatg ctcgcaaaat gggatatgaa tgtacgcaga aaccttgttt
    21721 tctgcgggat ttattgtttc tatcattgaa attctttctc cttacgtcga aaagtcgtgc
    21781 ctgttttcga ccttcttaaa atcgcgttca agcaccaact cattgaaata agccgcatat
    21841 ttagaatcaa ggaaacctag ctctaaaaat gcttgcttat tgtcatcgga gctacacggt
    21901 gcttttacag cctctcgata aacggtaatc gcaaagctga ccttcggcat taagtcaaaa
    21961 tctgcaaaaa tatgaaaagc tattttcaca gattcctcct taagtgctca gaccacaacg
    22021 ctttgcttac aacctctcat atacgcatac taaacgtatt tggcgaacaa atccactaaa
    22081 aaatccaaga atcgacttct tggatgattt tttctttcat taaactggtt acctagttat
    22141 atatctctag ggttgttttc atgtctgagt gcccgatatg atttattttt cgtttctata
    22201 attacttttt gccttccagt ttcttttaat attgtactca agcaagtggg aattggttct
    22261 tttgtcatcc atgtatgcaa aatgttataa tgctggtaaa caaaaatgat tacctgtcat
    22321 ttttatttag ttcgtattta attaagttga ctatctgtat actctgtcta aaacttaaga
    22381 attgttgttt tcgctgtcgc tacccaattt taatgaatgt ctgtaggtta aaactgttgt
    22441 ccttgttata gatagtaaag gagagcttga aaatgaaaac tgcgattacg tttctattag
    22501 aggaatatgc cgattgggaa ggtgcttata ttactagcac actaaaccaa aatgagaaat
    22561 ggcaaaacaa aactgcttct atccaatcag aggtcacttc cattggagga ttaaaaacaa
    22621 aaatcgatta cttaatcgaa caaatacctg aaaaagttga cctttttatt atgatcggtg
    22681 gcaattcatg ggacataaaa aatcatcatt tatatcaaat aatcgataaa ttgttaaaaa
    22741 cagacgtacc agtagctgct atttgtgggg cagttgatta tttagctcag agcggattat
    22801 tgcaaggtta caaacacacc ggaaatgcgc aatttccttg gttagaatcg gaaaattatc
    22861 agaataaaga agattttata gaacaacaag ctgtgaaaga tcgtaatttg attactgcga
    22921 atggaacagc tcctatagaa ttcacagact tgattcttca agcagtccat tttgaaacag
    22981 aagaaataag agaaaaaacg attcgtctat acaaacttgg attctatgat tactataaaa
    23041 agtacggaga tcctttctcc taatcattct cttcaattaa atactcctta tcaatagtgt
    23101 gacaagtaat tttactgatg attatttgtc acactttttc tgacatttat caaagaagat
    23161 acactctatt tatattcata gacaaggttt tacggattca aaaattcagg attatgctat
    23221 actggtatta aaaaaataat gaggtatttt atatgaaaaa gttaggtttt attttattgt
    23281 tggtctcttt attccttgta ggatgtacgt ctcctagtaa agaaaatagt gatccatctg
    23341 cgaccacaga caaaagttca atcagacaaa ccagttcagc tgaaaaatca agtactcaga
    23401 aatcagcttc tactgaaacg aatacatctt caacaagcag taaagtctca gagcccaata
    23461 cttcttctac atctagtagt acagttgaga caaatatttc ttcttcaaca atagacacaa
    23521 cctcctcaca gtcttctcag tcaatagatc cgttagcagg atattctgat ctacaaattg
    23581 agtacgcacg tgtttggcta gctattagag gtaatacttt tagagataca tcgggtgatt
    23641 tcgaattaca tgttattcat tatcctgcag gaacacctat cgccccttat gatgcaggga
    23701 gtgcagtcta tccgaaagat gtggttatgc tcactgggaa atacagttac cagggagttt
    23761 tagtgtattc aagtaaccat gacgggacaa tcactagtta tccagttcct tcacattggc
    23821 aaatgcctgc agatcaaaca tcttatccag cgttcattag agaaacaact caagaaatca
    23881 ttgataatgc atctgtagta gctattccta caggaaatcc caatgacgtg aaacagttaa
    23941 tcgatgtgac agttatagat aattagactt tctaatatgt tatacaaaaa gaagctgaca
    24001 gcattttgtc agcttctttc actttctttt ctatttagat ttattggtct ttgatagact
    24061 ccaaggcagg aaaaaatttt ttaaataaac gtttgcaaaa cagtcgcttt tctatcttct
    24121 accgctactt ctgcataggc accattaatc aatgtcagtt gtggcggtac atgaccacaa
    24181 tcaatatcat acaagatcgg tagttttaat tcctttgaca gatcttcgta tacatccttc
    24241 attttataat ctaacaaatc ctctgccgca cttctaccga acatgatccc cgagctgcca
    24301 tcaaaccagc ctgccagttt catctgaaca agtgatcttc ttaaatcaac tgacgatagc
    24361 tcacagtttt ctagaaacca caagatcggc tttccatcat tatactttct tttaaaagat
    24421 tggacatctc cataaggagt accgattaaa tgcctgatcg tatcaataca accacctaat
    24481 aatcgtccct tcatccttac tgcatctccg ctgactgatt gccaatttgt tggttcagtg
    24541 agatggaaaa cagaagatga tgaatgctca tggggccaca ctttttgata cttttcagaa
    24601 gattgctgta gaatcgattc tccttctctt gttttcagta cactttccca cattgctgtc
    24661 gtttcatcca agtactctcc tcttaaatca ataaggttag ttccatgtgc agtggctatc
    24721 cctgttttta atgtgacagc aagtagcaac gtactaatat ctgaataacc tactatccat
    24781 tttggttgta gagcatcaaa atcgacaaaa tcgagaatct caatcaataa ttctcctccc
    24841 caagtcggaa agatcatttg gatattttca tcttgcatca tttgattgaa ttcatctgct
    24901 cgttttcttg ctggacctga tttttcctta tcttgcgtcc aaagcgtctt tccagtaact
    24961 acttggaaag atttttcttc cattctcttt aaagctgatt cgagtaacga atggtgttcc
    25021 tctgatacgc ctgaggatgg tgcagtaacc ccaatcgttg catttctttc taataatgga
    25081 taacttatca tgttgcttca cctccaagta tgcactattt tacttggtta attgtttttt
    25141 tcgtttgccg gcatcaaaaa tatcggcatc tcaagtagct ggatgatttt gtttaagtca
    25201 accgtttgga tgaccatcgt ttgatctggt ctacctggtg caaaaacaag tttttcattt
    25261 gataagagct cttcatcaat aataatcgga atctgttttt cataaccaaa cggagaaaca
    25321 gcgccctgct tttgtcccgt ttgttcttcc atgcgttctg atgaaacaat agaaagcctt
    25381 ttaccgacga tcttccctaa tgctttgaaa tccgtttgtt tcgtagtaaa agtcagatat
    25441 acgtagttat tatctttttt atcttttaag aataagcttt tggtctctgt acctgtaaat
    25501 ccttgttctt tctttattac caaagcatct tcatttgtat aaatggctcg atgatgatac
    25561 aactcaaaat atatattttc gtgtaagaat ttctttgcca agtcaatcat ttttatgtct
    25621 cttttctatt ttattatttg agacatctta tcatataaat atagcggtta caacattact
    25681 tgtgttttgt tcgtttttta tacataacta ttgtttttct ggtaaaataa atcaaaccac
    25741 ttaatggagg cgttttagaa tggaaaaatt tcagccgatc atcaatcaaa tcaaagaaaa
    25801 agaacatcaa caaaagatca aagacctttt tgctaagata acagaaacgt atccccagct
    25861 agacacaact gtaaaatgga accaacctat gtttactgac catggtacct ttattattgc
    25921 atttagtttt gctaaaaatc atttttctat cgcaccggaa aaagcagcga ttcgtgccct
    25981 tgaaaaaaat atccaagaag caggctacgt atatacggat aacgtcatca aagttccttg
    26041 gaaaagtgta atcaactggg aattgattga acaattgatt tcatttaata ttgaagataa
    26101 aaaaggtcat gataaatttt ggcgataaag ctttatacga ataaaccttt tactggcgag
    26161 gaaactattc atcaccttat acaggttttg ttcaatccta tcctttttat catccctggt
    26221 taaacactta attgacaaag tatctgctat ttttataagg agtctttctt gtgctgaaaa
    26281 aaatcactga ttgaatggct ttgaattttc tacttagtaa aattattttt tcagcaattt
    26341 tcctgcaaaa cttttgaata aggaaaaaat acgagaaaat atcagtgatc tttcataata
    26401 ctgcccatta ttttcaggta tatttaaata taatatcact ttaggagaga acgaatttct
    26461 ttagagaaat aaaagttaga attttctgac ttgattttta caatgacctt aattaaaatc
    26521 gttctttcta catttcgtga ttaagcaaat atcaactaag tcagtagaac cattcacata
    26581 ggagggtgaa cattgaaaga tttttactca gtaaatgaat tagccgaaca attaggtgtt
    26641 actacacgtt ctatcagaaa ttatcttcat gaaggaaaac taaaaggaac aaaggttggc
    26701 gggcaatgga aattttctga acgtaatctt tttgagttcc tatacggaga tcaagcagag
    26761 gaagcagcaa aagaaatgca gcgattcatg ctaaatgcac caatcacgat gcgtttcaac
    26821 cttcaatatc gtgattttac agcaatcaat caattccgag aacaattagt ccagtaccat
    26881 aacgatgttt acgcaaataa aaaagaccgt cttttacaat acgatcttta taaagacaat
    26941 catgcggaga tactaatcgg tggtaacttc aattatgtcg taaactttag tcaatggatc
    27001 aatgaaaaat tattgatcca gacagatatt tcccttgtat cttaaacaat taaaaagcgc
    27061 tagaaattcc agagatgttg gaaattctag cgctattttt ttataagtac ttactgtttg
    27121 gttcaaacaa atattttttt aataaatgaa attttaccat ctttatcctc aaataaagac
    27181 cgtgaccatg cttcctacta cgatcagcaa taatccgata atcgttaatt ttttttcttt
    27241 tggtgtctta ttttcatgca ggatgtacat accgcctaat gtagagataa ctacgtttag
    27301 ctgtgtaatg atatatgcag tagcaacccc attttcattt gctgaaaaaa tataagccaa
    27361 tgctgacaca ctaaagatgg ctccaacgat cgttgctttc caactcttgt cttcagaaaa
    27421 tgcggttggt ttacggaaaa tcacataaat cactgcccct aaaaagacac cgatcatttg
    27481 cggaaagaaa atggaaagtc cgcttgcatc aactagttta ggcaatgcac tgtataccca
    27541 ataaccaata cttgttgatg ctaatagact aattcctttt gtgataccgc ctttttttcc
    27601 aggatcatca ctgattgccg ttaaagcaga accaaataca agaagaatga ttgctgctgc
    27661 tccaatgatt ttccccatac ttccagacca ttctccaaaa gcgaagacac caatcaacga
    27721 ggttccgact aactgtaatg cagtcgaaat tggcatcgtt ttagaaacac caatcaagtt
    27781 gaaagatgta tactgtcctg tttgtccaat aacccaaaat gcaccagaaa gtaaactaat
    27841 caaaaatgta gtcactgaaa taccagctgg agaagcgaat aattgaacga gtaagccgac
    27901 aagcaccgct ccaataccag ttcctaatat ctcatttgtt ggtctgccac caatcttttt
    27961 caaaatcagt ggctggatcc cccagccaac tgccgggatc aatgcaatca ataaacccat
    28021 ttttattcac ccttatcctt ttccaccttg gaaagaagga tataaagtca tcccaccatc
    28081 tgcaaatagt gtaatacctg taacataact ggattcgtct gaagccaacc atgcagctgt
    28141 cgccgctatt tcttttggtt cacctacgcg tcctagtggg atcatacttt ccaagagttc
    28201 tttttgttca ggatcatcaa attttgccgc attaataggt gtacggatag caccagggcc
    28261 tatactattg attctgattc ccttcggtgc atactccatt gctaccgttt cggtaaataa
    28321 ttttaccccg ccttttgaag cagcgtaatg agcaaacgtt ggccatggga tctgttcatg
    28381 aacagaagac atattgatga tatttccttt tttattattc tctgtaaagt aatttaaagc
    28441 cgctttcgtt ccgataaaaa cgccagtcaa attgactttt aacactcttt cccaatcttc
    28501 aaggctaagt tcatgggttg gatgctgatt ttccatccca gcattattaa cccaaacgtc
    28561 cagtgtccca aaattttcta aagccgtatc aagtaaacgc tgtattcctt cttcagaact
    28621 gacatctgct tgtacagaga ctgcttttcc gcctgcctct tcgattgctt tgactgcttt
    28681 ttctgctcct tctttatccg agttatagtt gacaactaca ttcatttttt ctttcccgaa
    28741 tcgttgagca atcgctgttc caatcccctt tgagccacca gttactactg cgactttgtt
    28801 ttctaattcc ttgtacatag tacccactcc tttttcatac ctaaacctgt tattacggat
    28861 aacttttatt tccgccttaa agtgagtgta caccaagata tatagtctaa agcaaatgat
    28921 acgctctagc aatcgttttc atgaattgct agtgtccttt tatttctata aagaaagagg
    28981 ttgtgacaga agtggtcagc ttcaagaaat ttcggcttat ttccgaagaa gctacttctg
    29041 aaaaaccatt tatttggaaa aaaaggctgt gacaagactt ttgtcacagc ccctttcctg
    29101 ggcttattta tcacccattt tattgtaatt gctagttata cataaacaag agattttatg
    29161 cataatgttt tattgctgag aaaaatattc agctgttgct tgttcatcct ccttgagttg
    29221 ttcaatcaat tcctctacac tagaaaattt cacttgatct cttaaaaatt gattccagcg
    29281 taccgcgaca tgttcaccat agatatcttg attaaagtcc aaaatataca actctacagt
    29341 cagctcccgt ccttcaccga atgtatcatt atgtccaatt gaacccatgg ctggatacca
    29401 tgtttcccca atttttattt ccgacacata aacaccttct ttaggcaaat gaaccgtgct
    29461 ctttactttg acatttgctg tagggaatcc tagtagtctg cctcgtgcat cgccatggac
    29521 aactacccct tctacctcat atgtgcaacc taatagtttt gctgcttctt ttacttgtcc
    29581 gctgtctagt aatccacgaa tacgggtaga gctgattttt tcaccttgtt cttcttcttt
    29641 agaaactgtg acgattttga atcttccttt ggcatattca ggaaaatgag cgacatctgc
    29701 aatgtctttt ggtccatacg tataatcaaa accggaaaca gcatattttg catgtaaccc
    29761 tacgatgtat tgatcaacaa attcctgcgg tgctaaatga gcaaaagaag aagtaaaatc
    29821 aacttcatat aaataatcta tacccaaagc ggccattttt tcttcttttt gcttaaggct
    29881 agtcaaatat ttcacgctgc taggatcgat ctttttaaag acaatagatg gatgctgatt
    29941 gaaagtcatc aatgctaatt tcaatccttc tttttgtgca atttccatac ctgtctttat
    30001 gactttctgg tgtccgatat gcacgccgtc gaaaaatccc agcactaaaa cgacatcgtc
    30061 ttctggaatt tgttctgatt ggtaaggatg tctgatcttg atgatattca ttcgttattc
    30121 ctcatttctc aatactttac taggttttaa tttgtttttt tctttcgggt ttggctgata
    30181 aatactgaca acttttcctt tataaaacaa agcaatttct tctgacggca tttctgggag
    30241 cgcaaataca gtataatcaa gtctcatacc gttttttact ttctgccata cttcatcagt
    30301 aatatctact cgtttgaacc gttccactcc tttttctatt ggcagcaaac aatcatcgat
    30361 tgtcccattt ttcacagctt gagctacttc ttctaatgtc agagcatcct tctgggacat
    30421 accggcactt gctgttcggg tcagatcaga catgtgcgca gggtaaccaa gtttcttgcc
    30481 tgtatctaca gctaatgtgc ggacataggt tcctttcccg cattctactt cgaagcgcca
    30541 agaaatggtt cctgtcactt cgtctcttgt caattcactt gtccggataa aacggtaaat
    30601 ctgagccttt ctaacaggac gctccactgt ttcattattg cgggcatatt catataaccg
    30661 cttaccgttg acttttaccg ctgagtacat tggtggtatc tgggtgatct caccaataaa
    30721 tgaagccatc acttcatcta tttttttttc agataaagac atatctactg gttttgtttc
    30781 tacccgttct ccggatcgat cttccgtcgt tgtactgaaa cctagagtta tctcgcctac
    30841 atagattttc ccagaatctg tcaaatattc gataactttt gtggcttttc caatacaaat
    30901 gggcaacaca ccgtctacgt ctgggtcaag cgtaccccca tgtccgattt ttttcgtatg
    30961 taagattttt cttaatttga atacgcaatc atgacttgtc attccgcgtt ctttccataa
    31021 aggtaataac ccgtccatgt cttttctcca tttcattttt gatttaactg aattattata
    31081 acacattgca tcaattactg acgaatttga catcataatg aacggttttt atttttcggc
    31141 aataaaaacg taacaaaatt catgctcacg taaatagaaa tcatgttatg cttttttaaa
    31201 ggagggaata gaatggaaga ttttttcttt gcaccatttg attggattta tgggtggctt
    31261 acaggtaaat agtttggttt tcacttccca aaaacgaaaa aaaagactgg ccattcttcc
    31321 agtctttttt ccgttttttt tatttatttt ttcagtttct taatcaatgt aaaaaattct
    31381 attttgcggg atcgatttta tcattttatt ggtcaagatt tttccatttc caatccaata
    31441 cttctggcag atcaatacca ttttcacgaa tatagttttg atgttcttgt aatttctgat
    31501 ccattttttt cgcaaaatct tctgcttgac ttcctttgat ttttaaagct gcgtcttttg
    31561 ccaaatgata acgatcaagt tcatttacta cacgcatgtc aaatggtgtc gtgatatctc
    31621 cattttcttt gtagccatga atcattaaat gatggttatg acgatcaaag aaaatcgtgc
    31681 ggaccaatgt ttcataaccg tggaaagcaa ataagatcgg tttgtctttt gtaaaatatt
    31741 gatcaaattc ttcatcgctc aacccacgcg gatcttgact tggatgacgt aatttcaata
    31801 aatctacgac attgatgaac cgaattttta gttttgggaa ttcttccagc agcaaagtta
    31861 cagctgctaa tgcttctaag ttagattccg ttcctgcagc agcaatgacc aattcaggtt
    31921 cctcctcttt tgctgtcgaa gcccaatcaa tgattttcaa gccttcattc actaagacgg
    31981 ctgcttcttc tgctgagtaa aattgagcac gcggatgttt ggaagagatg atcaaattga
    32041 ttttttctga cgagcggaat gctttatcca tgactgcgag caatgtattt gcatccgcag
    32101 gcagatattc acggatgaac tccgccttct tttctgccaa atgtgtcaat attcctggat
    32161 cttggtggct atatccattg tgatcttgct ggaagactgt tgagcttgca atgagattta
    32221 gcgaaggata atcatttcgc caagaaaggt catgtgattt ccgcatccat ttgaagtgct
    32281 gagtcaacat agagtcaacc actcgcaaga atgattcgta acttgcaaaa aaccatgccg
    32341 tccagtcaga acgtaccctt ctaagaaccc ttcatcttgg tgttccgata attgactgtc
    32401 gatgacgcga ccagatggcg aaagccattc atcattttct ggttcgattt tttccatcca
    32461 ttggcgattt gtcgttttga aaacttgatt caatcggttt gattttgttt catctggtcc
    32521 aaatatacgg aaattttctg gatttttctc aatgatctcg cgaacgaatt taccaagttc
    32581 ggtcatatct tctgctttaa cggagcctgg tttttcaaat tgcaaagtat attttttcca
    32641 atctggcata ataagcggtc ttggatcgat tccaccattg gtgataggat tttttgccat
    32701 tctttggtca ccagtaggcg cgatttctct gatttcttct aaaatacgtc cttgtgcatc
    32761 aaataatttt tctggttcat aggatttcag ccaacggagt aaagcatctg catgatccat
    32821 atgttcttga tctacaggaa tcggtacttg gtgtgctcgg aaggttcctt caattggttc
    32881 cccgtcccat tcttttggac cggtccatcc ttttggtgtg cgtacgatca atactggcca
    32941 ttttggcatc acgacatctg ttgctgtctt caggcgagct tctttttgga tggatttaat
    33001 tttctcgatt gcttgatcca ttttttcagc cataacagga tttaattttt ctgaatcatt
    33061 tccttcaata aagaaaggtt cccagccaag tccgttaaag taatttgcta attctccatc
    33121 tgatttacgt gccaagatcg ttgggttggc tatttttgca ccatttaaat gaaggatcgg
    33181 taaaacagct ccatctatca ctggattgat aaagacattt gagaaccagc ttccagctaa
    33241 aggaccggtt tctgcttcac catcaccaat cacgacagca gagattactt caggattgtc
    33301 taatacagct ccgactgcat gtgaaagaga gtatccaagc tctccgcctt catggataga
    33361 acctggtgtt tctggtgctg catgagaagc aatgcctcca ggaaatgaaa atcgtttgaa
    33421 taatttttgc attcctgtct catcttccgt tacttctgga tagatctctg tgtaacttcc
    33481 atctaaataa gcattcgata ccatgacttg tccgccgtgt cctggccctt caatataaaa
    33541 catgttcaaa tcatatttat tgatgactcg gttcaaatgg acataaataa aattttggcc
    33601 aggaatcgtt ccccaatgac cgattggatg ttttttgaca tcttcgggtt tcagtgtacg
    33661 tctcagtaag gggttatctt taagataaag ttgcccaaca gaaagataat tagcagcacg
    33721 ccaccaagcc gtcattttat caaagtattc tttactagaa taatccatac ataacactcc
    33781 ttttcattat tactcttatt ctagcgcaga tactcccaat tgaaacctaa taagcacttc
    33841 tgttcatttg tttggatttt atatctagcc aaatgaacag gtataaaaaa ccacgaaatt
    33901 tttcctttcg ctgacgtttt ttacattgat ccaaaaaact tgtcgttatc acaatggctg
    33961 ttccgcagtt catttcgctc ctcatcatgc ataatttgct gaatgacgct gggccagtta
    34021 acacattgct ggaaaactgg ggctggatcg agcaattcct ttcctgattg atccgtttta
    34081 tgtaaaagtc tctgttatct ttgtcaatat gtgaattggt atcccagcta ctatgctaat
    34141 ttctacaggg attatccaaa atttggatcg tgaccaaatc gaagcggcgt aatcggaaat
    34201 atcaataact tcaacgtgat ttttctttta actgtcaacg gaccgacaaa ctcaaacttt
    34261 tatggtgcag gttccactga tcttctagtt acttggctat acctgactgt cactaaaata
    34321 gattacaatc tagcttccgt tatcggtatc ttgatcttta tctatatgca aaaatttata
    34381 tactactaga aaaaagaaag tcgaattttt tacttttctt tttctctttt ttggttagct
    34441 gacacgccgc aagactaaag tcatggaact cttaggtaat acgtcttaat gaaaacaaaa
    34501 aaatcccgca acccctgatt tcacaggatt tgcgggagtt tttgattagt ctttgttcaa
    34561 gtcgcgaagc aattcatcga tatggttgcc atagacaact gattcatcaa gttcaaagaa
    34621 caattcaggt gtcttataaa tacttagatt gtgccctaac tctcgacgaa tcaatcctga
    34681 tgctttattc aatccatctt gtgctgtttt cttatctgat gctaagtcag ataaaatgct
    34741 gtaatagatc gttgcttgct gtaagtcccc cgtaactttc acatctgtga tcgttacgtt
    34801 ctccacacga gggtcacgga cctttttgcg caaaatatca ttgacttctt ttaagatttc
    34861 ttgaccgact cggcggtcac ggtagttagc cataatgatt ggcctccttt tatcagtttt
    34921 tagtcatttt tgacttcttc catgataaat ccttcaatta cgtcatctac tttgatgtca
    34981 ttgaattttt caatcattgc accacattca taacccattt tgacttcttt cacgtcatct
    35041 ttgaatcgtt tcagacttgc aagttggcct tcatagatta cgattccgtc acggattaca
    35101 cggacaccgc tatctctacg aatatatcct tctgttacat aggcacctgc aatcgtacca
    35161 actttagaaa ctttgaaggt ttcacgaacg atcatttgac cggtgatttt ttcttcaaat
    35221 tcagggtcta gcatcccttt catcgcagtt tcgatttctt cgatggcttt atagatgata
    35281 cggtgaagac gaatatcgac ttcttcagta tcagcttgca cttttgcctg cggtgtagga
    35341 cgaacgttga aaccgatgat aatagcgttg ctggctgctg ctaaggttac atcactttca
    35401 ttgattgctc cgacagctga atggacgatt tttacacgga caccttccac atcaatcttc
    35461 ttcaatgatg cagccagtgc ttctgctgaa ccttgtacat ctgctttgat gattacgtta
    35521 acttctttca attcgccttc tttcaggctt tcaaataagt tatccaaagt cacacggctt
    35581 gtggcagcac ggttttctaa taatgcacgt ttcgcgcgtt cttcacccgc tgcacgtgct
    35641 gtcttttcgt cttcgaagac tacgaaacgg tcacctgctt gaggtacatc attcaagcca
    35701 gtgatttcta ctggcgttgc aggtcctgct tctttatcgc gacggccaag atcgtttgtc
    35761 attacacgga cacgtccgta agtattaccg acaacgattg ggtcaccaac atgaagtgtt
    35821 ccttcttgga caagtaaggt tgacacagga cctttacctt tatcaagacg tgcttcgatc
    35881 actgttccga ttgcgcgttg tgttggatct gcttttaaat cttctacttc tgcgactaac
    35941 aggatcattt ccagcaattc ttcgatgttt tggccgaatt tcgctgagat ctcaacaaag
    36001 atcgtttctc caccccatgc ttcaggaatt aactcatact cacttaattc ttgcataaca
    36061 tgttgagggt ttgcacctgg tttatcaatt ttatttacag cgacgatgat tggaacgcct
    36121 gcagctttgg catggttgat tgcttcaact gtttgtggca tgacgccatc atctgcagca
    36181 acaactaaga tcgtgatatc tgtgatactt gctccacgtg cacgcatact tgtaaaggcc
    36241 gcatgtcctg gtgtatccaa gaaagtgatt ggtttaccat caatatcgat ctggtaggca
    36301 ccgatatgct gtgtgattcc gccggcttcg ccgcttgtta cacgtgaatg gcgtaatgta
    36361 tccaataaag ttgttttccc atggtctacg tgtcccataa tcgttacgac tggcggacgt
    36421 gagactaatt tatctggatc gacttcttct gcctcgaaga atttatcgat atcagcaatg
    36481 tcaacttgga ctttttcttg tggttccatg ccatagtccg tcgctaacag ttcaatcgta
    36541 tctttatcca gtgcttggtt ttgattgacc atgacaccaa gcataaataa ttttttaatg
    36601 atttctgctg gctcacgatg aatttttttc gcaatatctg ctacgttcat accttcagta
    36661 tactctaata catctggcaa ttcacggaat ttgcgtggtg gtacagctgg tttagttgat
    36721 tgttgctgtt tgcctttttt acctttttta ttataacgat tacggttatt attattaaat
    36781 ttgccgcgat tgttattgtt tggtttgttg ttattgttag tacggttttg attgccgcct
    36841 tggctcgttc cttgacgatt ttggctgctg gtatttcccg tattattttg gtttgttgtt
    36901 ggtttttttc cttgattcac ttgaccgctc ccttgaccgc gatcttgata actgcggtta
    36961 tttttatttt tttgttgatt gttcgttgtt tcttttttac gttgatcgtt cggctgttga
    37021 ttggctggtt ttctgcttgc ttgttgtgca gcttgttgtt tcgtgcttgg gtttttgaac
    37081 gcttgttgca atttcttctc atcattgcta gtaatcgcac ccatatggtt tttcacatcg
    37141 atgccaagtt gttgtgcttt ttccacgacg tctttactag attttttgag ttcttttgct
    37201 aattcataga ttcttttgtt gcccatgcaa tcaccttcct aaccttcaat tagctccttg
    37261 gtcttcttcg caaatccggc atccattatt ccgaatacct tgcgaggttt tccgatcatc
    37321 tgcgtgattt ctgcttcaga aaatagctca aagcaaggaa cttcataata tgaactctta
    37381 tcttttatct tttttcttgt attctcgctt gcatcggttg ccacaaacac caattttgct
    37441 ttttgtttgc ggatttcagc aatggtcagt tcttcacccg tgattagttt tcccgcgcgc
    37501 atcgctagac cgatgagatt catggctttt tgccgattaa ctgtattcat tttccaaata
    37561 actctttacg tgctttttgg tgagtgacat agtcaagaag ttcttgataa aactcatctg
    37621 ttaatgttgt ttcgagtact cgatcgagaa tttttttatc ccaagcttgt tgtacttctt
    37681 ccggctcaag tgagacatac gctcctcgtc caggcatttt tccagttgga tcgatcgata
    37741 cttccccttc ttttgaacgg gtgattcgga ccatctcttt tttaggtttc atttcacctg
    37801 aaaccacgga tttgcgtaaa ggaattttcc tttgtttcat ctctttgcct ccttactaaa
    37861 gttaagcttc tggtttttct tcgaccgttt cattgttgaa tgcaattgtt tcatattcat
    37921 cgtctgttaa atctgactga acgattgctt catcatgcag ttcttctgtt tctttttgtt
    37981 gttcttgatt ttcataaaat tccgtcatgt cagattctga tttgatatcg attttatggt
    38041 tagtcaattt agcagctaaa cgtgcgtttt gtccgcgttt accgattgct agcgataatt
    38101 ggtagtctgg aacgacaact gtgcaggcct ttggatttgt ctcatcgaag atcacatcca
    38161 tgacttgtgc aggattcaat gcatttgcaa tgaatacagc tggatcttca ttccattcga
    38221 cgatatccat gttttcgcct tttaattcat tgacgattgc ttgaactcgt tgtccctttg
    38281 gcccgacaca tgtaccgact gcatcgatat ttggatcatt tgagcgaact gccacttttg
    38341 aacgatcgcc agcttcacgg gcgatgctga caatttcaac gattccatca tagacttctg
    38401 gtacttcttg ctcaaataag cggcgcaata ggtctggatg gctgcggcta acaaatactt
    38461 gcggaccttt agacgtattt tctactcggg aaacataaac tttgattcga tcatgtggtt
    38521 gataaaattc atttggcatt tgatcttgtt tagaaagaac tgcctcaatt ttaccaagat
    38581 tgacataaat ataacgttta tcctggcgtt cgacgattcc ttgcatgatg tctttttcat
    38641 aagcactgaa ttcgttataa ataatcgttc tttctgcttc acgtacacgc tgtaaaataa
    38701 cttgtttagc cgtttgcgca gcgatccgcc caaaatcttt tggtgttact tcaaaacgga
    38761 tcgtatcacc gatctcatat gctggattga ttaaaagtgc atctttcaat gagacttcca
    38821 actgtgaatc catcacttct tccgttactt cttttaccgc atagacatgg atctttcctt
    38881 ttttctgatc aaattccact tcgacgtttt gtgcttggcc atagtgacgt ttgtatgcag
    38941 aaaccaatgc tgcttctaaa gcatcgatca cgatttcttt tgagatccct ttttcagctt
    39001 ctaaagcgtc taacgcattt aacatttctt tactcatttt ttctgattcc tctccgtgat
    39061 taaaattgga tagctaagcg agcttttgca atattttttc ggtcaaaagt caattctttt
    39121 tctcgtgttt ttatgcgtat tttcaatgta agctgttcag catcaaatga ttgaagaaat
    39181 ccttcatatt gtttttcgcc atctactggt tggtataagg agacatggac ataatcacca
    39241 agtgcctttt catagtcgct ttcttttttc aatggccgct ctgctcccgg ggaggatacc
    39301 tccaggaaat aagcttgagg aatcggatct ggatctgctg catcaagttt ttcactcagt
    39361 ttctcactga caaaggcgca ttcttcgata tcgataccgc cttctttgtc aataaatact
    39421 cgtaagaacc agctttttcc ttcttttaca aattcgactt cgacaagttc aaaattttgt
    39481 tcatctaaaa tcggtgtcac catttctgtg actgtttcaa cgacgctact caaaagtttc
    39541 gcctccttaa aaaattaagt gcgtaattcc attaaaaaga gtgagcgaaa aatccgctca
    39601 ctcacagtta gtatcattac ctataagaac tataccatac tctgcttaaa attacaagca
    39661 gacaggaaaa cagatgctta tagcatatcg aataacgata attgattttc atctggtaga
    39721 tctttcaata cgccattgtc gttcatatat tcgatcaatg tttttgaaac tttaccgcgg
    39781 gtggcaagat cttctttgga taaaaatgga ccgtcttttc ttgcttcaac gatttgtttt
    39841 gccacgttgg ttcccaaact tgggacagct ctaaatggtg cgatcaacgt atcaccttca
    39901 ataacaaagt taacggcatc cgatttatac aaatcaatca ttccaaattt gaatcctcgt
    39961 tccagcattt cattggctaa ctccagcact gtcagttgat tcttttcttt tacggaagca
    40021 tctaatcctt tgtccgtgat ttctttcatt gcttgtttca cagcatcttt tcctttgcac
    40081 atggagacta gatcaaagtc gtctgcacgt acagaaaagt atgcacagta atacaaaata
    40141 gggaaataaa ccttgaaata agcgacacga agagccatca atacgtaagc tgcagcatgg
    40201 gctttcggaa acatatattt gatttttgaa caagaatcga tataccagtc gggcacatta
    40261 ttttctttca tcgccgatag ataggtttct cgcaattcat cagggatctt gttccattgt
    40321 cctttacgaa cagtttccat gattttaaat gccatcccgc tatctaatcc agcatggatc
    40381 aaataaacca taatatcatc acgacagccg attacttccg ccagtgtcgc atctcctcgt
    40441 cgaatcaatt cttccgcatt tcctaaccat acgtctgtac catgagatag tccggaaatt
    40501 tgcagtagtt ctgcaaaagt tgtaggatgc gtttcttcta gcatccctcg aacaaaacga
    40561 gtgccgaatt ctggaatacc taatgtccct gttttggaat aaatttgttc ctgacttact
    40621 ccaagtactt caggaccggc aaatatccgc atcacttccg ggtcatccgt agggatcgtt
    40681 tgtggatcga tccctgataa atcttgaagc atccgaataa cagttgggtc atcatgccct
    40741 agaatatcta atttcaatac attatcgtga atcgagtgaa aatcaaaatg agtcgttttc
    40801 cattccgaat tttgatcgtc tgctggatat tggatcggcg taaaatcata gacatccata
    40861 taatcgggaa taacgatgat cccccctgga tgctggccgg ttgtccgttt gactccagta
    40921 gcgccttttg ctaatctgtc gacttctgca cttcgaaact gcagattatt gtccctttcg
    40981 tatcctttta catagccata tgctgttttg tcggcgactg taccaatcgt tccggcacgg
    41041 taaacatagt cttcgccaaa caatactttc gtatagttat gggcttctgc ttgataatca
    41101 cctgagaagt tcaaatcaat atcgggcact ttgtctccat ggaatcctaa aaatgtctca
    41161 aatggaatat catgaccgtc tttgtttaag cgtgcaccac attttggaca ttgtttttct
    41221 ggcatgtcaa atccagaacc atacgtacca tcttcaaaaa actcggaata ttggcactct
    41281 ggacaataat aatgaggggc taacggatta acttcagtga ttcctgtcat cgtcgcaaca
    41341 aagctagagc caacagagcc acgtgaacct actagatagc catcttcatt acttttatgc
    41401 actagttttt gggcgatcag ataaataacc gagaagccat taccattaat agaattcaac
    41461 tcttttttta atcgtttttg aatgatctca ggtaacggat ctccgtacat ttgttttgct
    41521 tttgtgtaac tcaattctga tatttcatct tctgaacctg gaatcttcgg tgtataaagt
    41581 tcatctttga ctggaatcac ttcttcgcag atatctgcga ttttattcgt attttctact
    41641 acgatctctt tcgctgtttc ttcccctaaa aagtgaaatg cagttagcat ctcatcggtt
    41701 gttcggaaat ggacatccgg caaactatga cgattcaacg gattcgctcc gcccatagaa
    41761 ttgatcaaaa tttttcggta aatggcatct tcttcgttca gataatgtac attacctgtt
    41821 gcaacaacga ttttatccaa actttttccg atttctacca aattttggat aatttcttcc
    41881 aaatcatgtt cattttttac caattcttgc tcgatcaaag gcgcatagac cgcttttggc
    41941 ataacttcaa tataatcgta gaattttgct cggtttcttg cctcttctac ccctttttgc
    42001 atcatggctt cgaatatttc tcccttatca caagctgagc cgatcaatag attttcacgc
    42061 atctttttca attgggagcg tggtattcgt gggacgcgtt cgaagtattc gacattcgac
    42121 attgaaatca gtttgaacaa atctttcagt cctgcctgat tttttgctaa aatcgttaca
    42181 tggaaaggtc gagccctttt gtaagaatct ccttctccga tatgctcatt tagctgatca
    42241 tgataaagca tgttatgatt gtccattgcc tctttcacaa agatccatgc taagtgacca
    42301 gttgcttcag catcatagat tgctcggtga tgctgttcca agctgacacc aaatttttta
    42361 gataacacac ctaatccgaa acgtttgaat tgaggataca gatatctagc taattccaac
    42421 gtatcaatga ctggattggc cgcttcagga ataccatacc ttgcgtagct agtattcaag
    42481 aaccccatat caaaagccgc gttgtgggct accaaaattg tatcttttga aaattcaagg
    42541 aacatccgca atacttcttc ttccgacttt gatcctcgaa ccattccatc cgtgatacca
    42601 gtcagatcta ccgttgttcg tgacaaagga tgtcctggat cgataaattc atcaaagctt
    42661 tcgatcacat ttcctttata cattttgact gcggctagct cgatgatcgt gtcatatact
    42721 gcagacaatc ctgtcgtttc cacgtcaaat acgacatatg tggcttcact caatgcttca
    42781 tgtgcatcat tataagcaat tgggacacca tcatctacga cattcgcttc cacgccgtat
    42841 aagattttca cacctgcttt ttttccagca ctatgtgctt ccggaaaggc ttgcgcacca
    42901 ccgtggtctg taatggcgat agctcggtgt ccccatttcc ctgcttgtgc cacaagatca
    42961 gagatgctgt ttgttgcatc catcgtactc atattgctat gaacatgaag ctctactcgt
    43021 ttttcccctt caggagcata atctttgcga ggtgtatgtt tgacttctat gatatcttgc
    43081 gcattcatga ccaaatcacg gacaaacgta tcttcttgaa tacttcctcg tacctttaac
    43141 cagctaccga cactgatcgc atcaaatact tgttcatctt tttcgccgtt cgagaatttt
    43201 ttgacgataa atgatgaagt ataatctgtg attttcaaag tcaagatctt gcgttttgaa
    43261 cgtagttctc tgacttcttt gtcgaatacg tacccctcaa gtgtgactct tcgttcttct
    43321 tcaacgatac tgatcattgg tgtcgttggt tcatcggctg gaatatttcg tcctagctgg
    43381 atgtggatgg gtccttcaag agctggcgat tgctcttttc tttccttttt cttttgttcg
    43441 tggacaacta gactttcagc tgcttgcttc ataaaggctt ctgcctgttc ttgtttgcgt
    43501 tcttcaaaaa gtttcaatac tcgttccgct tgctgctcat ccacttctgg ttcgatacga
    43561 aattttggga atccgtaact gacgaacaac tcttctacta atggcaagta ttgttgcttc
    43621 aaataagaaa tagctcctgt actgtcgatt ggcaagatga ctttacgatc ttttatcaca
    43681 ggtacctgcg ttttcaatac ttgctgtgct agtggtgtat cgcattgctg attttccaaa
    43741 gcttgggccc agtaatcttg caagagctgt tcatcgaatg tctggtcatc tgctaagata
    43801 ttcagtttga cttgtgcaat atctttaaaa gcgatagtca gatgctgagt caatgaccga
    43861 tatagcataa taggcaaaat tttggaaaaa tgcagcgtaa attcccataa acgcgattta
    43921 cgatggacca ctacactatc catttcacca gcagtaagca gtggatgatt tttttctgct
    43981 tcctctagtt ggatttgttc caacagtttt tcaaataaat ctcgtttttc tgacaaagat
    44041 atacgccctt tctgatgaga aacaatgcaa ttgatcgcat cttatcctct tctttttcag
    44101 taccttttag tatactcgtt ttttctgagt tttacattaa aaaattggaa ctgcgattca
    44161 aatcgcagtt ccaattttaa aagcgaatca tcagattgtc aaaaacagct gattcagcta
    44221 tttttatttt ctgtgtttta acgcttatta ttcgccttgg ttcaagagga ttggtaaagt
    44281 attcaccaat tcttctttac gtacttcaac catttcgcca gttttcttga ttttcacttc
    44341 tacgattcca tccactgctt ttttaccgac agtgattctg attggacagc caatcaaatc
    44401 agaatccgcg aatttcactc ctgcgcgttc atttctgtca tcaaccagta cttcatagcc
    44461 agcttttgtc atcgcttctt ctagttcatt cgtcaggttt gtttggtatt cgtcttttag
    44521 attcatttgg acgatatgca aatcaaatgg cgcaattcct tttggccagt tgattccttg
    44581 ttcatctgaa tgttgttcta cgattgctga taataaacga cttacaccga taccataaca
    44641 gcccataatc acaaattttt ctcgtccatt ttcgtctagt acagttgctc ccatagattc
    44701 actgtaacga gtgccgattt tgaagatatg tccgatttcg attcctcgag taaaggcaag
    44761 aacaccattg ttgtctggtg aaggatctcc ttcttgtaca aaacgaagat cttcataaga
    44821 aagtacagtg aaatcgcgtt ctggattcac atttttatag tggaatcctg tttcgttcgc
    44881 tccagtaatg gcattcgcta aatcttggac ataacggtct gcatatactt ttacgtcatc
    44941 tgaaacaccg actggtccga cagaaccaaa ctcagcacct agatattttt gtgcttcttc
    45001 ttctgttgct tcttctaaaa agtcagcaga taaaaagttt ttcagtttta cgtcattgac
    45061 ttcatggtct ccgcgtacca aaacaaggac aggttgttca tccgcaatga acaatacaga
    45121 cttgatgatt ttttgaggtt caacagaaaa gaaatctgct acttcgtcaa ttgattttac
    45181 gttaggtgta gctactttct ccaattcaag ctgtgtttca tgcgattttt ttgatacata
    45241 gtagcttgta gccatctcca agtttgcagc ataatcactt tcagtcgaat aacagatcgt
    45301 atcctccccg atttctgaga tcgccatgaa ttcttttgag tctttcccgc ccattgcgcc
    45361 accgtctccg ataatcgagc gaaattctaa gccacaacgc ttgaatactt cggtgtaggc
    45421 tttttcgtaa tctttatatg tctcgtccaa actttcttcc gttgcatgga aagaatacgc
    45481 atctttcatg ataaactctc ttccgcgaag caaaccgaag cgaggtcgtt tttcgtcacg
    45541 atatttaggt tggatttgat aaagatttaa aggtaatttc ttatatgaat tgatttcatt
    45601 acgcatcaaa tcagtaaaag tttcttcgtg agttggtcca aggatcattt ttcgatcatt
    45661 gcggtctttg aaacgataca agtttggtcc atatgtttcg taacgcccgg attcttccca
    45721 aagttctgct ggcaagatgg caggcatcag catttctacc gctccgattt tttcaaattc
    45781 ttcccgcata atgcgtttca gtttttccaa tacacgattt gctaatggca gatatgaata
    45841 aatacccgct gatacttggc ggatatatcc agctcttagc aagatttgat ggcttaatac
    45901 ttctgcatca tttggaactt cgcgtaatgt tggaatcaac atttttgatt gtctcattct
    45961 atatactcct ttatttcctc aaaatagtta tcgtttgtta ttatacccta tttgtcaact
    46021 ttttctagaa aaagaatcgt tgaatatcgt tccatgtaac tagcaccatc aacaacatta
    46081 aaaatccaaa gccagctaat gtcaatattc cttctttttc ttgacttaat ggtttcccgc
    46141 gaatcccttc aaaaatattc aagactaatt tcccgccatc taaggcagga atcggaagca
    46201 gattgacgat tccgagattc attgaaagaa gcgccatcaa actaataaca gttgttacac
    46261 cttgatttgc tgcttctgaa gacaattggt acatcatgac aggtccgcct aatttatcta
    46321 aactgaaacc tgtaaatagt gatcctaagg ctttgaaaat ttccaatgaa ccgctgaaag
    46381 cttgacgcgt ccctccaatg atcttatcca taaaacctgt attcattggg gcttttatac
    46441 caagttggcc aactttttcg ccatttgact ctacagattt cggtgtcacg tcgacagatt
    46501 gcacctgacc ttctcgttcg atcttaaagt ctaatgtttt atccggattt ttcgtaatga
    46561 ctgttgtcag atcattccat gaatggattt cttttccgtc cacactgact acctcatcat
    46621 tttcttttaa gcctgcttct gccgcagctc cattaggcat gatctctcct acgcgattag
    46681 tgttcgtcac ttgaacacca ccttgcataa atgccagaat gatgaataac aggattgcca
    46741 aaataaagtt attcatgggt ccagcaaagt tggtcagcat acgctgccac aatttagccg
    46801 attggaactg tacgtctttt ggcgcaattc tgatttctgt tccgtcgttt tcaataatcg
    46861 tcgcatcgtg atcaacaggg taggttacta cttctgtttc gtctccattc acataaccag
    46921 tgatagtcag ctcatcttct aaatcataac ggctcaattc catcgggata gcatttgtca
    46981 attggatttt cttacttaaa tttatcttct ctacgatgcc gtcagaattt agtaaaagag
    47041 aaagaggcat tcctggtgcc atttctgttt catcatcccc attgcctgcc attcgaacat
    47101 aaccgccaat aggtaatagg cgaagcgtat atgttgtccc gtcttttgct tgatgcccat
    47161 aaattttcgg acccataccg atagcaaatt cgcgaacgag gattcctgat cgtttcgcaa
    47221 agaagaaatg accaaactca tgaacaatca ctaatatacc aaaaacgatg ataaatgtca
    47281 gaatcgtttt catagctttt ttctccttta cttgtttttt gtgtttttta taaaacaacg
    47341 ataaaacact ataattttac catagtatac caaagacacc aatagtatca atataatgaa
    47401 aaataaaaac ctgcgtcttt tatgaaattc acaaagaaac gcaggtatac ttactaaata
    47461 atgccaaata aatgcatgat aggaaataca aacagcaaac tgtcgaaacg gtctaagatt
    47521 ccaccatggc ctggtaagat atttcccgag tctttcacat cataatgacg tttgattgca
    47581 gattcgacca aatcaccaaa ttgtccgact gctgaaaaaa taatcgtcat cagaagcatg
    47641 acaaacatac catgattaaa tagttctttt gctgggtata gcaataaata aagaaatgcg
    47701 actacaacag cactaccaat accaccgagc gcaccttcaa tcgttttgtt gggcgaaata
    47761 tcaggactta acttatgctg accataacgt cgaccaacca tatatgctcc gatatcagtt
    47821 gcccaaacaa taaatagacc aaatatcaaa acagccaagc tttctgttcg tgcattgaca
    47881 aaattctgga aacctattcc cacatacaag ctgacaagca caggaaaacc tgcttcatca
    47941 atcgtatacg tatttttaga aataactgat gttcctaaaa gaatcataac tataagataa
    48001 aataacataa atgtacttac attctctgga agaaagaaaa accatctttc ttttggcaaa
    48061 acaagtaaca ctgcaccaac ggcggataaa actccttcaa aacttaagag tgtcaatcct
    48121 ttcatccgaa acagctcgta aactcctatt actgctaaga ttgctgctgt aagctcaacc
    48181 gctatgcctc cgatccatat aatcggaata aacaagacta gcgccacggc tgctgtgacc
    48241 acacgttgtt tcatgactga tcccctttct catcttctgt tttttttaca ccgccgaatc
    48301 gacggtctcg attttgataa gaggcgattg cttcttcaag atgcgcacca tcaaaatcag
    48361 gccaaagcgc ttttgtaaaa tacagctcac tatatgcgat ctgccataac agaaaattac
    48421 taattcgctc ttctccactt gtacggatca tcagctcagg atcttgtaac tctttaggca
    48481 agaaacctgt cattaaatgc ttagcaatca attcatcatc gatttcttct gcgtgtattt
    48541 ctccttttgc tacttcttcg gcaatttctt ggacagcagt cacgatctct gctctgcttc
    48601 cataattcaa agcaaaattc aataccattc cagtattgtt ttttgtttgc tcaattgcac
    48661 gtcttacagc atcttgcgta tgttctggca atacgttttc ataacccatg acatgaactt
    48721 tgacattttc tttaatcaat tctggtacga acgtatcaaa gaagtcgaca ggtagttgca
    48781 tgagaaaact tacttcatct tttggtcgct tccagttttc agtcgaaaag gcataaagcg
    48841 tcaacacttt cactccaagc cttgatgctt tttttgtgac tttctttact gtttccatgc
    48901 cttctttatg gccagcaact cgtggcaaac gacggttttg cgcccaacga ccattgccgt
    48961 ccatgatgat ggcaatgtgc tgaggaattg gtccatcggt attaaattga aattcgcttt
    49021 tttcttgaat atatttgttt ttttgcggaa aaaaacgtaa cattcttttt cctcctatac
    49081 ttaaaagatt cccactatct taagtattat agcaatatct aactgttttt cctatgaaat
    49141 cattgttttt ttatttaaaa actgaataat tttttctttt tttaattttt aaataaaaga
    49201 aatataaata aaaacagagg aagcggaatg atccgcccct ctgtttttat gattttatta
    49261 aacttccaat aattcttttt ctttatcagc agcgatctta tctacttctt tgatgctgtt
    49321 atctgtcaat gtttgtacgt ctttttctaa tccgcgcaaa tcatcttcgg taatatcacc
    49381 atttttttgt tgttttttat attcatcgat cgcatcacga cggatgttac gaatcgcgat
    49441 ttttgcattt tctgcttctt ttttaacatc tttagctaat tcttttctgc gttcttctgt
    49501 cagttgcggg ataactaagc gaatgacatt tccatcattg gttggactga ttccgatatc
    49561 acttgccata atagcttttt caacatcttg aagaatgctc ttgtcaaaag gtgtgatcat
    49621 taaaacacgt gcttctggaa tttggattga tgccatttgg ttcaatggtg ttggcgcacc
    49681 gtagtagttc acagtaatac ggtccaaaag gctagcgttc gcacggcctg cgcggatttg
    49741 cccaagttca cgttgaagat tttgtgctgc ttttttcatt ttttcttttg cttctgtcat
    49801 aattgcatct gccattttta tttccccctt acagttgttc cgatattttc gcctaagatt
    49861 gcacggcgaa tatttccgtg ttcatttaaa ttgaagacaa gcagtggaat atcattatcc
    49921 atgcttagtg aacttgcagt tgaatccatg acttgtaatc ctttggcaat aacttccaga
    49981 tgagtcagtt cttcaaattt tactgcattt gcatctactt tcggatcagc agaataaaca
    50041 ccgtctacat tgtttttagc catcaggata acatctgcgc cgatttctgc cgcgcgtaat
    50101 gctgctgttg tgtctgtcga gaagtacggg ttgccggtac cgcctgcaaa gataacgatc
    50161 cgtccttttt ctaaatgacg ttctgcttta cggcgaatat atggctctgc gatttgacgc
    50221 atttcaatcg aagtttgcac gcgtgtcggt acacccacgt tttccaacgt atcctgtaaa
    50281 gctaaggcat tcataactgt agctaacata cccatgtaat cagcttgagc acgttccatg
    50341 cccatttgtg ctccgatttg tccgcgccag atatttccgc cgcccacaac gattgccatt
    50401 tcaacgccca gctcgtgaac ttctttgatt tcttggatga tttctttgat tactggtggt
    50461 ttgattccaa atcctgcgtc cccagctaaa gcttctccac tcaattttaa gactacacgc
    50521 tgatatttcg gtgtgaccat gttcattcct ccaatatttt ctattgccat tttaccatac
    50581 ttagatgatt tcgacactct aagactggtt tttcttgttt tgcttaaaaa aagggagcgc
    50641 acaaaccgtg cgttcccgtc tgttattcaa cagaattatt ttttcacttg gctcataact
    50701 tcttctacaa agttatcttc gcgtttttcg ataccttcgc caacttcgaa acgtacgaat
    50761 gttttaacag ttgcgccttt tgatgcaacg tatttttcaa cagtcatatc aggatcttta
    50821 acgaatggtt ggtccaccaa agcgatttct gctttgaatt tcttcaagcg tccttctacc
    50881 attttttcaa cgatattcgc tggtttgcct tcgttcaatg cttgttctgt aagaacagtt
    50941 ttttcatgtt ctaattcagc ttctggaatt tgtgattcgt ttacataacg aggattgata
    51001 gctgctacgt gcatagctac gtctcttgca actgtttcgt cagttgttcc atctagaaca
    51061 gtcaatacgg caatacgtcc gcccatatgc aagtatccac caaatgcagc attgtcgtct
    51121 ttttctacaa cttcaaagcg acggaagctg attttttctc cgataacttg agttgcttcg
    51181 atcaagtcag attcaattgt acctttatct gttttgattt tcattgcagc ttccatgtct
    51241 gctggtttgt tttcagcaac aagttctgca atttctttta ctaaatcttg gaacatttcg
    51301 tttttagaaa caaagtctgt ttctgagttt acttcaacga ttgctgcagt gttacctttc
    51361 acagcaactg aagccaatcc ttcagctgca atgcggtcat ttttcttcgc agcctttgcc
    51421 attccttttt cacgtaaaag atcaactgct ttttccatgt cgccttctac ttcaactaat
    51481 gcttttttcg catccatcat tcctacgcca gtcatttcgc gtagttcttt taccatttta
    51541 gctgtaacgt ctgccatgtt taactcctcc tgtgtgtttt tctttttaaa aaaagctgct
    51601 tcaaaggaga gtgaggcttg tcgcccagcc tttgaaacag ctccatcatt caaaattatt
    51661 ctgctgaaga attgtcgcct tcaacaacat ctacgatttc ttcgattgat gttgctgtgt
    51721 tttcttctgt gaaatcttct tcaactactt gatcttcacc ttggtttcct tcgatgaaag
    51781 catcagccat ttttgaagta atcaatttaa cggcacgaat tgcatcgtcg tttgaaggga
    51841 ttactacgtc gatttcatct ggatcgcagt ttgtatcaac catagctacg attgggatat
    51901 ttaatttgtg tgcttcttga acagcaatac gttctttacg tgggtcaacg atgtacatta
    51961 catctgggat tcttggcata tcagcgatac cgcctaagaa tttttcaaga cgttcacgtt
    52021 ctttgttcaa accagcaact tcttttttag gtagaacttc aaaagttcca tcttcttcca
    52081 ttgcattgat ttgtttcaaa cggctgatac gtttttggat agtatcccag tttgtcaatg
    52141 ttccgcctaa ccaacgatgg ttaacgaagt attgtcctgc acgagttgct tcttctttga
    52201 ttgcttcttg tgcttgtttt tttgtaccta cgaataatgc tacgccgcct tcttcagcaa
    52261 cgctcttcat gtaatcataa gctgcatcta ctaatttaac tgttttttgc aagtcaatga
    52321 tgtagattcc gtttctttct gtgaagatat atttcttcat ttttggattc cagcgacgag
    52381 tttggtgacc aaagtgtacg ccggcttcta gtaattgttt catagaaatt actgccatgt
    52441 ttgtttcctc cgatttggtt ttatttttcc tcttcttatc ttctaccttg ccggcaaact
    52501 ttttcaagca cctaccgaca atccaataag aatgtggatt tgctgatgca taaagcaccc
    52561 tcagctatta tacagacaaa agatccgtat cgcaagagca aatatacttt tctgctcaac
    52621 attcaatagt ttacctgaaa gataaaaaga attcaagaaa aacagtagat ttctctttgt
    52681 ttctttatta tcctttttcc ataaaataat tttaggtata aaagacgaat attattgggc
    52741 aatccttctt ttttttgtta tgatacatgt gaaatctttc gtaaaggaga aagaaaatgc
    52801 gcaaagctca aagacatttg ctgattaaac aagtcattga agaattcccg atccgtacgc
    52861 aagaagaact attaaacaaa cttgggcatt atggcgttac cgccacgcag gcaacgattt
    52921 ctcgagatat ccgtgatcta aaaatcgtga aggcacctga tgaaaatggc gtttcccgct
    52981 tcgtattgtt tcgcgaaaaa ggaactcctg agactaggac tgaggaagag gatcggctgg
    53041 ttcaaatgat tgaagacatt gtattgaaag tcgaacgagt ccactttttg acgatcgtca
    53101 ataccttgcc agacaatgct catttatttg catctgtttt agatgagatc aaacctccct
    53161 ctattgtcag tacaattgct ggattcgata cgatcatggt catctcggaa aatgaggagg
    53221 acgcaaaacg agtagaagaa tttcttcacc atccaactga agtatcactt ttttaatcag
    53281 agcacgtatt cctaatatgg aatgcgtgtt tttttatttt caatattttc cttattgaat
    53341 aaacctttac actctttaca aaaatacatc attttttatt caaatttttc aacaccttga
    53401 ttgaatcaca ataatcatca tttatattgg atgttaacaa gagcagcttc ttgctctttg
    53461 tgtatttcaa aaaagaaatg aaaggaaatc atatgtataa attattaact tctggaccat
    53521 tgacgacttc tgaaacagtg aaaaatgcta tgctgatcga tcattgtact tgggatcaag
    53581 aatacaaaga aattacacaa tggataagaa aaaaattatt ggatcttgct caagtatcgg
    53641 aaggaatata cacaacggtc ttaatgcaag gtagcagctc cttcggggta gaatcggtat
    53701 taagttctgt gatcaggcca gaagatactg tactgatttg ttcaaacggc gcatatagac
    53761 aacgaatgat caacatgtgc gaatgtctga aactgaacta taccgtttat gaggtatcgg
    53821 aacatcaaat cctccaagct catgtgatta cacagctgat tgaagagaat ccgtctatct
    53881 ccgcactagc aatggtccat agcgagacta cttccggtat tttgaataat ttggaagaga
    53941 ttgctgctgt cacacaagaa agagatcttt tgtttatagt ggacgcgatg tcaagctttg
    54001 gtgggatacc gatttgtgta gccgaactag ggattgattt tttgatttcc agctcaaaca
    54061 aatgtattca aggtgttcca ggcttttctt ttgtacttgc taataaagcg gaactggaaa
    54121 aacgaaaaaa tgttagccga agtttgtcac ttgatctata tgaccagtac cgcgtgatgg
    54181 aaaacgagaa tggcaaatgg cgtttcactt cccctaccca tacggttttg gcttttcaac
    54241 aagcgctgaa ggaactagag gcagaaggag gaatcgaaac tcgatattca cgttatctcg
    54301 ccaataatca tgtgctacga caaaaaatga cagagatggg gttccagcct ttcattcaag
    54361 aatatcaggg gccattcatc acctcttttc tttaccctaa ggactggcga tttgatttta
    54421 atcattttta tcagtattta aagcaaaatg gatttgtgat ctatcccggg aaaatcagcg
    54481 atcatgaatg ttttcgtatc ggaaatattg gcgagatcta tccggaagat attttggaac
    54541 tgacagcaat tatcaaagac tataaggaga acgttaatga ttaaaactgt aatttttgat
    54601 tgggcaggaa caactgtgga ctttggctgt atggctcctg tccacgcttt tagaaatgct
    54661 tttctagaaa aaggaatcca actgactgac aaagaaatcc gctaaccgat gggaaaatta
    54721 aaatgggatc atatccaaca gcttctttcc ctcccttctg tcaaaaaaca atggatagct
    54781 atttatggac aactacctca ggaaaaagag gtggaggaat tgtatcaaca atttgaacat
    54841 tatctgttta aagatttggt caagcattct accttgaaac ccgacacatt ggaaacgatc
    54901 gaacggttga gaaacgctgg gatcaatatt ggttccacga atggatttaa tgctcctatg
    54961 atgacaatcg tagcagaaac cgcaaaaaaa gaaaggtatt caccagactt catggcaact
    55021 tttgaagacg tcgaagggta tggtcgaccg tttccttata tgatccataa aaacatgcag
    55081 tacttcaaaa acaagtcggt tgatgaagta atcaaagtcg gagatacggt ctcagatatc
    55141 caggaaggga aaaatgccgg agtgttgaca gtaggtgtaa ttgaaggtag ttcgctaatg
    55201 gggatttctt atgatgagta ccaaaaatta gagatgggga aaaagatcgt ttaaaacagt
    55261 ctgttgctct tagattccaa caagccggcg ctgatatttg tgtaaacaat ttaaaagagt
    55321 taacagaata cattttgtac gctgacatga atccagaatg acttttatac aaaataagag
    55381 gttgtgaaaa agctgtcctg tatcaagtaa taaaaacagc caagcaaaaa tagctcctca
    55441 tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac cgtttattcg
    55501 aaaaaaaaaa gggagctgtg acacgacttt tgtcacagtc cccttatttg caaacaaaca
    55561 gtgtttaaga gacaacctct ccactaaacg gtgttcattc agctgactct cggtatttgc
    55621 tcaaaaaacg ataaaatttg aaaagctatt ttctcttttg tttctctaat actcggatca
    55681 gaacgctgat ttcacattct ctttttctta catcttatct ggtgcatgaa gtcctagcaa
    55741 acgaaggtct tctttcaata atacagtcac tgcataaact agagctaagc gtgcttcttt
    55801 ttgttcatca tcagctaaaa tcttcgtatg agcatagtat ttgttgaatg cttgtgctaa
    55861 tttgattgca tgtttcgcaa tcactgacgg ttcgtatttt tccccagcag acaagaccgt
    55921 ttcagggtat ttttgaacta atttaaccac ttcccagctg gtatcgtcat tcaatgcata
    55981 gtctgctgtt tctgaaggga caaatcctgc tttttccaac aggctgactg cacgagcatg
    56041 tgtatattgc acataaggtc cagtttctcc ttcaaatcga acaacttctt ccaggttaaa
    56101 gtcaaatgta ttcagtcgat cgtttttcaa atcgtggaaa atgactgccc caaccccaac
    56161 ttgtttagct acagcatctt tgttttccaa atcagggttt ttctcgctga tttgttcttt
    56221 agcagattgg attgcttcat ttaatacttc ttctaataag acgatctttc ctttacgagt
    56281 tgataatttt ttaccacctt gcgtgatcaa accaaatgga atatggtgca tgtcgtcaga
    56341 ccagtcaaag cccatttcct tcaagacagc tttcaactgt ttaaaatgat agctttgttc
    56401 attccctact acatatagag attgtttgaa gtcgtaagta cgtttacggt acaatgcagc
    56461 agctaagtca cgtgtaatgt atagtgtcgc accgtctgac ttcttaatca acgctggatt
    56521 caggtcataa gcagataggt caacgatctc tgcaccttta tcctcgttca gcaaatgttt
    56581 ttcttctaaa agtttgacga tttcatccat cttgtcattg tagaaagctt caccgttcaa
    56641 cgaatcgaaa cgtacttcca gcagatcata gattttattg aattctttca tcgattcatc
    56701 acggaaccat tgccataatt gaatagcctc tttatcgcct tcttccagac gtttgaacca
    56761 agcacgagct tcttcattta attctggttc tgtttcagct acttcatgga attggacgta
    56821 taaacgaagc aattcattga ttggttctgc tttaacagct tcttctgtac cccatttttt
    56881 gtaagcgacg atcagttttc caaactgtgt tccccagtca cctaaatggt tgatgcggat
    56941 tggctgataa ccgatttttt ccataatgaa accgatcgaa ttaccgataa ctgtcgaacg
    57001 caaatgtccc attgagattg gtttggcaat attaggagaa gacatgtcga ttggtactgt
    57061 tccttgattc ccgatgttgc tatccccata atgttctttt tctttcacta ctgtttgcaa
    57121 aacttttttg ctgataagtt ctttattcat gaaaaagttc aagtaaggtc cgacaacttc
    57181 gattttttca aagttggcag aatcgatttt ttctgctaaa tctgcagcga tttgctgtgg
    57241 cgcttttcgg taaacttttg ctaatgaaaa ggctggaaat gcaacatctc cgtgttcagc
    57301 tgattttgga ttttctagta attgctcgac ttgttccagt gttaaatcat ctttgacagc
    57361 gtcatagaca gcttttgcga caagatcttt attattcatc tttattcctc ctaaagtgtt
    57421 cccttgattg ttaaacaaaa aaaatctcta gcgacggcat acttagtatg ccatcactag
    57481 agacgagatt tcccgcggta ccactccaat tgtcctaaaa aaggaccagc tccatctgat
    57541 atcggtagac gaccgtctgt ttaaaacaga attctccaag tgcgcttcgt tgtatcttct
    57601 tcattcggct tccaccactt ccgaattcgc tagccaagaa tgatacaagt actctcttgt
    57661 tcaacaaatc ttagtcttat taaactttct tgaattatat cagaaaaaaa aacgaaaggc
    57721 tagtcgtttc ataaaaaaac aatccagatg gcctaaagga gaagaaatac cacctggata
    57781 gctataaatc atttcgtaaa tacgaaagac ttaacaaatt aatcagctac gataatcgtt
    57841 ccttcttcag aagctaaaag attctcgata ttttctaaag aagtgatgat tgccttagcg
    57901 tttggttttg cttcaacaaa tgcaatagct gcttctactt tcggaagcat gcttcctgga
    57961 gcgaattgct tttcatcaat gtattgtttc atttctgaaa cagtaacagt ttctaatttc
    58021 ttttgatctg gtttttggta attgacatac acattgtcta ctcctgtcaa tacgatcagc
    58081 aaatccgcat cgatgatttc ggctaatttt tctgaagcaa aatctttatc gattacggct
    58141 tctcgtcctt ctaatccagt agctgtttct acaacaggaa tcccaccgcc accaacagag
    58201 actgtgataa ctccttgatc gaccagcgtt tcgatgacac gtgcttcttt aatcgagatg
    58261 ggtttagggg aagcgactac ttttctccat ccacgtccag catcttcttt gaatgtcaca
    58321 gtggagtcag cattcatttg ttctttggct tcttcttccg tgtagaatgg accgactggt
    58381 ttacttggat ttttgaatga aggatcattt tcatccacaa taacttgagt gactaaagaa
    58441 accacatctt tatcgatccc tttttccttc agtacttcgc ccatggcgtt ttgtaaccag
    58501 taaccgatag atccttctgt cattgcaaca catgtatcta aaggcattgc cggtgttttc
    58561 tcagaatcag cagcttgctg ctgaatcaat aggttaccga cttgcggacc atttccgtgg
    58621 gagatgatca attcatctcc ttgttcgata aatttcacta aatactttgc tgtttccatt
    58681 aacgcttctt gttgggcttt tgcacttgca tccgtagaca agatggcatt gccccctaaa
    58741 gcgacaacta cttttcgttt ggccatttca ttcgtccttt cttcagttct accgaatttt
    58801 ctagataaca aaacgagcca gcgttttgtg attttccctc aatgctgact cgttttttcg
    58861 tgagatgaat gtggttatgc ttaaacgcga gggataaaca agttacctaa tgttgctgcc
    58921 atgatcgctt tgattgagtg catacgattt tctgcttggt caaattgacg agcatatttg
    58981 ctgcggaaga cttcatctgt tacttccatt tcagtgatgc caaagcgttc tttcatttgt
    59041 tcaccgtaaa cagtgtttgt atcatggaaa gctggcaagc agtgcaagaa gatcaaacga
    59101 tctgtgttgt gtgttttttc taccatttcc atattgattt ggtaaggttt caacaatttg
    59161 atacgttctt cgaatttatc ttcttcaccc attgataccc atacatcaga ataaaggacg
    59221 tctgctccgt ctacaccttt atcaacatcg tcggtaatca tcaattgtgc accggatttt
    59281 tcagcaaagc cttctgccat tttgacgatt tcttcttcag gttgtaattc ttttggtgca
    59341 acgatacgca tattcgctcc taaaattgca cctgtgacta ataaagagtt agccatattg
    59401 tttcgaccat ctccgcagta agcaacagtg ataccttcta ctgttccaaa gttttcttgg
    59461 atagttaaaa agtcagcgat catttgtgtt ggatgccatt catcagtcaa gccattccaa
    59521 actggaacac cagagaattc tgctagttcc tctaccattt tttgactgaa tccacggaat
    59581 tcgattccgt caaacatacg tcctagtact ttagctgtat cttctgtgga ttctttttta
    59641 cctaattgga tatcatttgc acctagatat tcaggatgtg cacctagatc gatcgctgca
    59701 gtagtaaatg cagaacgagt acgtgtagat gttttttcaa ataaaagcgc aatatttttt
    59761 ccttctagat agtgatgtgg gataccacgt tttttcaagt ctttcaagtg ttctgaaaaa
    59821 tcgatcaaat attgtaattc ttcttttgta aagtcttttt ctgctaataa gcttcttcct
    59881 tggaatactg attctttcat attaaacatt ctcctttaga ttgtttttat ttaagattaa
    59941 agaggaaatc ccgacggcag ctgacagtaa gactgccgtc aggaaacaaa ttacttcaag
    60001 tcttcacgga caagtggttg gctcatgcag cgtggaccac cacgacctcg tgacaattca
    60061 cttgaattga tttctaacac tttgatgcca tagctacgaa gcaattcgtt cgatacataa
    60121 tttcggttat aagtgactac aacacctgga gcgattgcca atgtgtttga tccatcattc
    60181 cattgttctc gaggtgcaac gatctcatct ccattaccag ttgggatcag tacaagatca
    60241 tctaatccaa gagcagcttt catcgtttcg tgtaagttat ctgaatgagt aatcttgata
    60301 tcatcaccat ctggttcgat tgtaaagaca tccatcttac cgtctttact ttgaattgct
    60361 ggatggatag tgaatttatc gtgatctacc attgtaaata cggtatccaa atgcatcatt
    60421 gcacggttgt tcggaatttt gattgccaac actttttcaa atcctgaatt ttttgcgaat
    60481 aggtggcgtg ctaatttttc taaagctttc gcattagttc gttgagaaat cccaacagct
    60541 actactttat cgcttaagat caattcgtct ccgccttcga tatgatctgg attttcacga
    60601 tccaaccata cttcgattcc tttatttgca aatcttggat gatgtttcaa aatatattct
    60661 gtaaacatcg attcacgacg acgtgcttca aaagtcatct tattgactgt catcccattt
    60721 cccattgatg cggaagggtc gcgtgtaaag tacaagtttg gcattgggtc catgtaaaat
    60781 ggatagtcat cgtctgctga cagatcatat agactgctag aacgtacttc gatatctttt
    60841 gtacgaaccc cagccatgat tttatctacc atttcttgcg tttccattga tagaaggaac
    60901 tcatgaagac cctctcttac agcattcgat gcaatatgag attcatccaa cattttattc
    60961 aagaattgtt ctttgacatt gcctgcgtca atcgcttctg ccgctaattt ttccaagtaa
    61021 agagtctcga cgccttcatt ttgcaatgtc ttcgcaaagt tgtcatgttc ttcttgagca
    61081 atcggaagat atggaatatc atcgaacaac agacgatcca taatatctgg tgtcaaattt
    61141 tccacttctt gtccaggccg tttcagtaaa actgtcttta atttccctat ttcagagaaa
    61201 acgtgaatag gtttatccat gttgtgttgc ctccttcttt ttgattacat gtataggata
    61261 tcctcctttt gtaatcactt tcataatttt gtctcattca aaatgagaca ttcctcacat
    61321 taatattcga taattaaata attaaacgtt tttctatttt taagcgtaat ttattcattt
    61381 tttattctgt atacactttc aaaaaaatat ttattcattg aaaaaatttt tttgaataaa
    61441 ttaaaaaaat aaattatatt atcgttgtta gcgtttacta tatgtattat tgttgttttt
    61501 tatacacaaa tcattcgctt caaaacaaat tattcaatac cattgtatat tttttacata
    61561 ataacaaatt atatttgtaa agtattaaaa cttgtttcta atgttggtat gttaaaactt
    61621 tacaaattct ttaaaaaaag tgacaataaa tatagtgaac aataaggaga ggacttttta
    61681 atgcgtgtta ataaaatcga ttttcacttt tcaaaactca gatatcaacc tgattttgaa
    61741 catttctcgg atgaagagtt ccaacagatc aaggaacata ctgttcttcg ttcttataaa
    61801 aaaggacaga tccttttcga cgatggtgat cccagacaac ggttgtatat cttgaccaaa
    61861 ggactcgttc gtttagaacg ttatgatgaa agtgcaactt attactacta tgactatgtg
    61921 aatacaaata acctttttcc tatgtcagga atgtttggag aagagaccta cacttataca
    61981 gctcaagcga tgactgatat tgaaacattc taccttcctg tttcctacta tgaaaaatta
    62041 gcacgaaaaa atcccgaaca gttgatctat atgatacaaa aaatttctaa agtagttgaa
    62101 atacatgaat tgcgtatcca aatcggattg acctctagcg cctttgaccg ggtaaaacaa
    62161 aatttattca ttttgaaaga agaattgggc gtaccaacaa aagatggtca tatcaccatt
    62221 ccttatccga tcaccttaaa agaattagca gtgaatagcg gcacgacacg tgaaactgct
    62281 ggacaagtaa tcaaacaact acgtgattcc gggctgttag attatgacaa aaagcgtttt
    62341 actttctgta agacagctac tactccagct atgagagaat aacgcgaaca aaaataagca
    62401 gtgaccctga taaatatcag ggtcactgct tattttcctc cctaaaactt ctgaaagatc
    62461 cggcagtttt agatcactgg acaagactca aagattttct atgtgtgttt cagttatgta
    62521 atattcactg tttgcaaaaa atactgttag attaactaga tgaagcgtct tgtcctcatc
    62581 cgatctagat tattcgatat tcctcgacaa taaacgatta ctcagtgagg gcaatcagag
    62641 gaacggataa tgataaataa gctgtaaaaa atagcaaaat aacaataatg attaaaagta
    62701 tgtaaaaaat agggctttga caaaaacgtg tcacagccct atttcgatta aacggtgtgt
    62761 acctgtcaac tcttcggtca caagtcataa tgttcataaa catgaggagc tgttttttga
    62821 aattgttcct tgtgaatcag agttaaacga caggcttacg aactcttgat taaacggcgt
    62881 tcaaaagctt gcatacgcgg taataagaac agccaggtca aaaacatgag aagccgtttt
    62941 tcctgggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct ttattcagat
    63001 aaaaccaagc ttggatcagc attcaactga tagctagcaa aatttctttt ttgcatttcc
    63061 acatgtgctg ctgctccaat cattgcagca ttatctccac aaagtcgaag agggggaatc
    63121 actagttcta cctcagggag tttagctgcc aaagcatcct gcaatccttc acgcaaccct
    63181 tgattggccg ctacacctcc agctataatc agttgcttga ctggataatt ttgacaagct
    63241 cgaagtgttt tagtaatcaa tacatcgatc acacttgctt gaaagctagc tgctaaatca
    63301 tttttgtcta gatcttctcc acgctgttgt gcattatgca ccaaattgat aaatgcactt
    63361 tttaatccgc taaaactgaa atcataatta tcttcatgga tcatcgctcg cggaaaatgg
    63421 taattatctt ttccctgatg agctagctga tcgatttctt ttccgcttgg gtaagaaaga
    63481 cctaatactc ttccaacttt gtcataagct tctcctgctg cgtcatctct tgtctcccca
    63541 atgatttcat atgatccatc ttcttgcata tagactaatt ctgtatgtcc tccgctaaca
    63601 agaagtgcca tcaatggaaa ctggaatggt ttaaccagac gggctgcata gatatgccct
    63661 gccatatggt ttacaggaat aagcggcaat tggtgtgccc aagcaaaagc cttagctgca
    63721 gaaataccaa tcagcaacga gccgacaagt cctggtccat aagtgacagc gactgccgat
    63781 aaatcttctg ccgacacacc agcttctacc agtgcatctt ccaagcataa ggtgatttgt
    63841 tctacgtgat ggcggctagc tacttcaggt actacgccac caaaacgttt atgactgttt
    63901 atttgagaag caacgatgtt agataagatc tctgttccat ttctaacgac cgctacactc
    63961 gtttcatcac aacttgattc aatcgccaga atcaattctt cattcatttc atcgtctccg
    64021 tttttaactt tctgctcata atgatcgcat cttccgtcgg attgcgataa tatgatttcc
    64081 gtttacctaa tatacgaaac ttttctgatt cataaagttt tcttgctgct tcattagata
    64141 gacgtacttc taaaaaaaca tgcagcgaat cttttatttg ttccgtaatc agtaattgta
    64201 acaaatgccg tgcatgccct tttccttgtt ctgaaactgc aacagcaata tttgtgatct
    64261 cgacttcatc tattactttc gagtatccaa taaagccaca gatctggtta tcttcagcta
    64321 aaagcagata atcagagtga ggctgttgga tgtctgccaa aaactgcatt ttcgtccaag
    64381 gtgaaccatg tggataagca ttttcgctaa tcatccatac gcattctgcc aagtacgctt
    64441 catcaaaatc tttttttctg caaatcaact aatctcactc attttctaaa agttttatca
    64501 tatccaacgc atcttctttt gtttcagtgt aatatctttt tttgatcgtt gttgcttgaa
    64561 accccaattt ccgataaagt ctttgtgcat cttgattact catacgcact tctaatgaca
    64621 tcgtctcaca gcgattcatt atagcaaagt tctcgatttc gtcaataagc agcgaaccga
    64681 ttcgtttacg ctgatatgcc ggtcgaacgg caatattcgt gatatggcaa tctaaaccga
    64741 acgctcttga accgataaaa ccaatcattc ttccattatc aaagatacct agatatagat
    64801 gaggaagcgg tgacttcaat tctgatataa aggcactttt tgtccatggc aattcgccat
    64861 tatagacttc tcgttcgaca tccagcagct ctttgatatc ttctgtagtc aattctctta
    64921 gtacgtaacg acgatcgatt tgttcgactg ttttttctgt ataaatcatt ttatcttgaa
    64981 aaaagcttct ggcatacgct ctaaattttt tcaacataac tttcgtctcc aggtgtgtgc
    65041 gtctccaacc atttttcttc tgcttctact cgttttaaat agcgaggaag aaaagcttgt
    65101 ttctctgcaa ctggttcaac taaagcacct aattgagcca cagttacacc acttggaata
    65161 tcccatgctg gaactgtatt aatcatcgca ctaggcaata tttcctttat ttcagagaag
    65221 aatttgtgac aatcactccc gacgaaataa atagcttcat tcagcttttg tagttgtgtg
    65281 aacaaatcag aagcagaaat atgtacatct tcaagtacag tttctaatct attttctttc
    65341 cagcggtatg ccccagcgta tacatttttt cttcgagcat caaacatggg cacaataata
    65401 ttatccacac cgatgcaatt agccgcgatg gttttcagac tagatactcc tactagctct
    65461 ttatctaatg tataagcaat cgtttttgct gtcgttacac ctatacgaag ccctgtataa
    65521 gaaccgggac catcagaaac cgcgattcgg tcaatgtctt ttggacttat ttgaagatct
    65581 gcaaacaatt ggtcgattgc aggcatcagt gttacactgt ggttgcgttt gatatttgtt
    65641 tgtatttgtc caagaatttg ttcgtcttct accacaccga tcgccaaagt ttggtttgca
    65701 gtatctattc ctaatgtaat catatatttg atcctttctg ctatgacatt ctcgccttcc
    65761 attgtagcat acttttttat tctgaggatg aaaataccaa aggcaaaact agtacaataa
    65821 tacatgatta tctgctggcg ggcaaatttt gccccttcct gaaacacaaa aaagctgcgg
    65881 tatcactcca ttttcccaca gcttttccat cttttatttc gttgattctc gaataatcaa
    65941 ctctgtttcc acaataatct ttcttgaaac cggtgctttg gaattactta attgaagaat
    66001 cgtctccact gctagctctc ccatccattc ggtataaact tttacagtag aaagcgaggg
    66061 cgttacatat ttcgctacac tgacatcatt gaagcccatc acagaaatat cttctggtac
    66121 cactaaacct ttttcttgaa tcgctttcag tgcgccaatc gctaatgcat cattagctgc
    66181 aaaaagcgca tcaggaacat gcggattcgt ttttaaatat tgcttcactg cttgatatcc
    66241 tgactcgaca gaaaaatctg cttcgataat tggaccttcg tcgtagcctt tttctgccaa
    66301 aacttgttga aatgccataa ttctgggatc ttttagccgc tgatggtttt tcttcgtata
    66361 ttctgttcca gcaagcatag cgatatgttg cgctttatgg gagacaaagt gttcaatgac
    66421 tttttgaaca cttgacttaa agtcgacaac tagagagtcg attccttgag aagtgccgtc
    66481 agaatctacc aaaagcaagg cattttggga tcctttcatt tctaataact gagcttcgtc
    66541 gaattttcct agtgcgatcg ttccatctgc cggaagatca ctaatggaat cccacgtttc
    66601 tttcaataaa gtaacgtttg cttcttctgc ctttttttcg attcctaggc gaatagataa
    66661 ataataaaga tcttcaagct cttccgtatc gtcgtaccac tggatcaggc gaagcgtttg
    66721 cttagtattt gctgcatttt tacgatgttt cgtataattt aagcgttctg ctgcttcaaa
    66781 aattttttgc tttgtggcta agccaacgga aagatcagga tcatagttga gaactcttga
    66841 aacagtagca ggagaaactc ctgccaactg cgcaatgtct ttaattgttg ccatttgtct
    66901 ctcctccttt tttacgttta taaaatcgca gtaaatcgtt caaacgctgc ctgtcctttt
    66961 tcatctcgct tgaatactcc agcgtcttcc agtacacgtg caaaaacctg acctacagat
    67021 ttttgtacga catcggtgac cgtatctttc gtcaaatcag gatgttcttt ttttaattgt
    67081 tctgcccacg gacgatgata ttcagccatc tggttttctt ctcctaataa gaaatgctct
    67141 acttctacta gttctggttt caaacgaggt ggtaaaacag caagccccat tacttcgatc
    67201 aacccaatgt tctccttttt gatatgttgg acatctttat gtggatgaaa aatcccatca
    67261 ggatgttctt ctgaaacatt gttgtctcga aggacaaggt ccaactcaaa gtaattgtcc
    67321 cttcttcgtg caattggcgt aatcgtatgg tgtggtgtac catctgctga aaaagctctg
    67381 atagatactg tttcatccga atatgctttc catttttcca atactgagtt agctgcttca
    67441 ataagcaagt ttttctctgt tgattgcaaa cggatcacag acatcggcca tttcacgact
    67501 cctgcgatga cttgcggata atcttctagt ttgataagtt tttctatcgg tgcttttgcc
    67561 ataggaaatt catgacgtcc tgcctgataa tgatcatgag aaagaatcga tcctccaaca
    67621 attggtaagt cagcattcga accaacaaag taatgaggca agacttcagt aatccgaagc
    67681 agtctagcaa atgtttcctt tgtgattttc atcgggcggt gttgttctga taaaatgatc
    67741 gaatgttcat tgtaataagc ataaggggaa tattggaatc cccagctttc accgtctaga
    67801 ttcattcgaa taatccggtg attggtgcgg gctggatagt tcaagcgacc tctatatcct
    67861 tcgttttcca tgcaaagcat gcaggtagga tagtctactt ttaccgcctc gcgttctgct
    67921 gcgatttgtt ttggatcttt ttctggctta gataaattga ttgtgatttc cagttcacca
    67981 tactccgact ctgccggaaa tttgatgttt tttgcgatgg ctcttgtttt gatgtagtca
    68041 tttttcttac ttaaattaaa gaaatactca gttgcttcct ctgggctttt cgaatagtgc
    68101 tgtgcaaaga atgcattgac gactgaaggt ggcggtgtga gaaaatccat taattgcgct
    68161 tcaaaaatct cttgttctgc tggcaattct gaaagaacac cattttctct tgccttagcg
    68221 acaagctgat ccaataagtc tacagattct tccttaactg gtcttacttc aatgctttct
    68281 agagcatctt ctcctaccat ccctaaaaga cgattctgta aatataagcg gtccatttcc
    68341 atccatccgc cagcttcgat cgcaagtgtg gcaaaatcac aaatcgtttg actgattgac
    68401 atatctcatt cccctattct tcataaccat ttggatggct gacatgccaa tcccatgctg
    68461 ttttgataat cgctttgata tctgtgtatt caggtttcca gcccagaatc tctctcgctt
    68521 tttcacttga agcgactaat cggctaggat ctccagctct tcttggtgct atttctgctg
    68581 gaatttcttt tcctgtcact tctctagctg cttctagcat ttccttgact gagtatcctt
    68641 tgctgctgcc taaattgaag aagttgcttt catttccttc tttcaagtat tccaaagcta
    68701 aaatatgtgc agcaatcaaa tcttctactt gaacataatc tctgatacat gttccatctg
    68761 gtgtatcata atcgtctcca taaacagcca aggcttttct ttgaccaaga gctacttgta
    68821 aaatgattgg gacaagatgc gtttctggtg tatgatcttc tccgatagat gcatctgctt
    68881 ttgctccggc tacattgaag tagcgcaaag cgacatagcg catgccatat gcttggtcac
    68941 accacttcat cattttttcc atcatcaatt tactttcacc atacggattt ttaggattag
    69001 ctggtgtatc ttctgtgatt ggagactctt tcggctctcc ataagttgct gcagtagaag
    69061 agaatacgat tttattcacg ttgaactcgt gcatgacttc aagtaagatt tgcataccgt
    69121 atacgttgtt gttgaaatac atcaatggtt tttcgacaga ttctcctact aaagagcttg
    69181 cagcaaaatg gattactcct tcaatcggtt ccttttcaaa tacggaacgt agaaattctt
    69241 tatcccggat gtctccttca taaaaatgag catctggatg tactgcttgt ttgtgacctg
    69301 tgagcaaatt atctaccaca gctacttgat accccttttg aactagctgg tctacagcgt
    69361 gcgaaccgat ataaccggct ccgcctagta ctaaaattgt cataaaaaac actcctttga
    69421 ttagtaaaat tgtgtttgtt gattctatat aatttaaaaa acgaagttta cttttatggt
    69481 gaaaacaaac aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag
    69541 taaacatttt caagggtttt ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt
    69601 tctcttttgt tccataatag atataacaaa taataaatga ggtggattca atgaaaaaat
    69661 ttgtatctgg aatattagta ggaagtctgg caacagccgc tgccgttgct ggtttggttg
    69721 cttctgtgaa aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa
    69781 accgtaaaaa agcaatgcgt aaacgcgttt ctcgttaata attatttaaa aatacgcatg
    69841 aagactttct aaaaaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag
    69901 ttgtgctatt ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc
    69961 tgcagttagc caatccacac cttcaacagg tattccatag caataaaagt cagactggat
    70021 ttcccctgaa gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg
    70081 ataacttcct gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt
    70141 ttttatgaca ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa
    70201 atagcgactg ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg
    70261 aatatcaaat ggcggttcga tcaaatgaaa aatacccgca tctatcagtg ctgctaattc
    70321 tttcgtccga atgattggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc
    70381 ccatagtatt tctttgtatt ccttagcaga aaaaacgtgc cattcaatag cctgccggat
    70441 tggatcacgc cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc
    70501 tttttccgct tcttttatct gcccttcaat aaatattcgg ctagcttctg aaaagggctc
    70561 attctgatag gcaagaggag tttctaaata cgaccaatcc catctataag ggattagtgc
    70621 tggatgcttt ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt
    70681 ctttggaatc ggcaagtgat attcttcgat gattttcttg taataaaaca actcaacgtc
    70741 ttttttcaga tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc
    70801 caggttttct tttgttagta accgtggcca tgccattgct ccgccttgct tttgatttct
    70861 tcctcgaggg taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc
    70921 tgatggatga tagaccagtt ttcccttttc gttctcgaag cgccctcctc ttgatactgt
    70981 aaacaatccg acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt
    71041 aggcggtatc ttcgaaagat ctacatctgc aggattagaa ggaagctgat acagtaaaac
    71101 atttttcttt gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg
    71161 accgcttgct aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata
    71221 accatctttt cttttgctta ctttttttac taaagaataa acaaactgaa tagaagctgg
    71281 aaattcttct tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt
    71341 agattgctga tctttttcca gacgattgat ttcttctata attagttgct tatttttggt
    71401 taccgtctgt tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc
    71461 ctgtgttacc aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga
    71521 aacagaattc attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc
    71581 attgggatcg aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct
    71641 ttctgctaca cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc
    71701 tccatttcga taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca
    71761 taaacaaaag taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc
    71821 ttcttttctt cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt
    71881 tctggtacat ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac
    71941 agatctaact gcgctttttc ttttgaaatt cctcgtggcc agaatctatc cacgagtatt
    72001 ctatagccat cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt
    72061 tccttctttc taccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa
    72121 atgaacggca caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg
    72181 attcggtttt taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt
    72241 ccagaccatc cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt
    72301 aatttatttg ctgtacggat gctcaaactt ttcttttgat cgtaaatata gacaggctgg
    72361 tcttttcgaa tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct
    72421 aaaatatgcc cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc
    72481 attccttctc gaaattcttc ttctgtcaaa gtcgttgctg aacgtttggc cattactcgt
    72541 agatagactt cccaaaggat gattgcaagc acaatcaata gcaaaatgat attaattacc
    72601 cataaaacca tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac
    72661 caatgacacc agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt
    72721 gcgggaaagt aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg
    72781 ccgatactcc tccacctaac acgatacttg acggatttaa tgtattcccc aagtttccgc
    72841 aagctaaacc taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt
    72901 cttgagccat ttcaaagata tctttacttg ttacttcttc tccgttgtct agcattgctt
    72961 tcagttttga atcccctgcg tattcttctg ccaagaaacg acctaggcgt accacacctg
    73021 tagcgctaga tacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag
    73081 gatccacagt gatatgtccg atttctcctc cagctccgct cacgccgtga atcaaatttc
    73141 cgccagcaac gatacctccg ccaacacctg ttcctagagt aacgaataca acgtctggat
    73201 cgttttctcc agcacctttc catcgttcac ctaaagcagc aacattcgcg tcattatcga
    73261 tagcgaattt gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc
    73321 agtttaagtt atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac
    73381 ccatgccgat tccgataaag tcttcggctt tcatattata taaattcaaa cgatgattga
    73441 ttgattcaat gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc
    73501 tccatttttg ctgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac
    73561 ctaaatcaat cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc
    73621 aattcggtgc tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa
    73681 acggctatca tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc
    73741 acccacatag ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc
    73801 catctttaag cacttccttc atattataca agtaaaccat atttttttaa acccagtaaa
    73861 atacatatta caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa
    73921 tagcttgtct gttgtttcgg tagacccaaa acattcccta acactaatcc gccaattagc
    73981 ccaccgacat gtccccaaac atctacagag gtatccagaa gaccaaaaac aaatgtcaac
    74041 acaataaata aaagaaaatg tctgactagc tgctggatcg ctgggttatg tttaaaatga
    74101 aaccctaaga taaacaacgc accgaacaaa ccgaatatcg aagtacttgc accaccagac
    74161 agaacattcg cttcgttaaa agcaaagctt gctgtatttc ccattatccc gctgaacata
    74221 taaatcaaga aaaaacgcca atggccatag attgcctcga tttgctgccc cataaaatac
    74281 agtatcagtg agttcattac gaaatgcatc agtccaaaat ggatgaagat tggtgtcact
    74341 aaacgccacc attcattgaa gtgaacaaga taaggaccat acattccggc ataataaggg
    74401 atctcaagtc gcggaaataa ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa
    74461 gcataggtcc ataacggtcg tcttaaccat gcacgcaatt ttatttgctg ttgataattc
    74521 atgaaaacct cccatttcat tttagtcaat aaacaattgt tggatcttct gatcaaagct
    74581 ctccggttgc cattgttcat tcaattgttc acgaaaaaca aggctacatg tttttttatg
    74641 aaaattattt aaataccggt cgtaataacc accgccaaaa ccgattcgat aaccggctct
    74701 attgtagata atacctggta caatgaggag atcgatcatc tccggcaaat agagcttctc
    74761 tgattgtggc tcaagaacac caaaactcga acgtacaaga acggactgtt catccatttc
    74821 ataaaacgcc atctgacgtt tcggcaaagt tttaggtaca actattttct tttgttcttt
    74881 ttctgctcgt tggataaccg gctgtgtatc cagttcaaat tcattcgata aagtcgtccc
    74941 tatcactttt gctgacttcc agtactcgct ggcgaatagc tgtttataga ttcgctgttc
    75001 tttttccttt ttcttgccac ttttggccag atttttcagc ttttcgattc ctttccggcg
    75061 taatcttact ttcaattggt ccaaaaactc acccttcctt ctctcataaa atggtatagg
    75121 aaaaaaagaa aaatagcaat cgaactttca aataaatgca taaatagaat aaaaagaaga
    75181 cctagaaatg attggccttc cttttttaat gaatttcaac tgttcagcgc gtgtttttgt
    75241 ctgataaaaa gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta
    75301 ggtacttccc cacttggtag aatcactcta gcaaccatat ctgcactaag cagaaaaatg
    75361 gaaccgaaca aaccgctcag aacaaaggtc cagttatttt ctttacgtac gactagcttc
    75421 gcagcatgag gagctagtag tcctacaaaa gataaactac ctgtcaccgc tacactaccg
    75481 cttgccaaag caacagctaa caacaaaagg attttttgac ttttagccac gttaaggcct
    75541 aatccttgcg cgcgttcttg accaaatgat agcaatacaa tcctctgtgc ataaagtcct
    75601 cctagcggaa ttaagataac cgcccatgga agcaatgccc aaacataagg ccagctcgca
    75661 ccccagattg atcccgctaa ccatgcattc acaaatccat aattttcctt agataattgg
    75721 atcgtgcata gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc ggacaataag
    75781 attcgattca tacccaaaaa gccatttcga tcataggccg caagatagac acagcaagca
    75841 gctaaaacac cacctagaaa ggctacaact agcaatagaa acggcagcga atagtgcgaa
    75901 aagaaaccta aataaagcat cactaaaaat cctgctccgg cattaatgcc caatatacta
    75961 gaatctgcca attcatttct tgttactcct tgcaagatat aaccagaaac gccaagacaa
    76021 gctcctgcta ataaactgat cagtaatcga ggaactcgaa attctctaac aatcagcgcc
    76081 aaagaagatg attctttccc tatgaacgtg gcaaaaagct gatccaggta aataaaaggc
    76141 gtaccaatca ttaaactacc tatagacagg atgacaagta caaaaagcaa gaaatagaac
    76201 gtccaatata tttttttcac cgttgttccc tccttacttg tatcagtaaa aacggaacgc
    76261 caatcaatgc aatgactaat ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg
    76321 taaaatccgc caacataacc aatccaccgc caactaaaaa agagacaggt agaacataac
    76381 gatagtccgt tccaaccatt ccgcgaacaa ctggcggaac caacagccca ataaatgaaa
    76441 caggtccgac taacgaaacg gcaatggctg ctaacactgc aactagacag aaaacaatca
    76501 accgcgttcg tttaggatga cttccaagag ctaatgcatg tgtttcactc aaatgaatca
    76561 aagatattgc ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca
    76621 atactgctaa ttgtttccat gtgatattgg cagctccgcc tacaaaccaa aaagcaagat
    76681 cctgctgcag attaaagaat tgggtaagtg cttggctgat agcagtaaaa aaagaactaa
    76741 tagctacgcc agctaaaatc agctgtaaag gattcatgcc cattttcgaa aactgcaaaa
    76801 aaccaaatac gagcaaagtt acagcaaaag aacctgccaa tgacattaga aacacagaaa
    76861 aatcagaagc tttaggccaa aaagcaaaac taagggccat tgccaacgaa gcacccgcgt
    76921 tgattcctag taagcctgaa tctgctaaag gatttcttgt cactccttgc atcaatgcac
    76981 cgctcaatgc aaaacaactc cctacaagaa atgctcccgc cacacgaggc aaacgaatat
    77041 ttcgtatgat ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat
    77101 ctgctttttc cacagtcgct gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa
    77161 caaaacagac caaaagcagg agtctatttt tcttcatagt cctcataatt gatcctcctg
    77221 ctcttttggc aacaaatcat aggttagaaa aactggttta tttgttcttg gaagcgtgac
    77281 aatcgttgca tcaatctcaa atattttttt catacactct tttgtgatca cttcttcagg
    77341 tgtaccttct gtgatcaact taccgttttt cattgctaat aaataatctg aaaaacgtga
    77401 agcatgatta atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt gattgatttc
    77461 agtcaataac tgtaggatct ctaattggtg tgccggatct aaaaatgttg tcggttcatc
    77521 caagatcaag atatctgttt cctgagcaag cgccatagcg atccagacac gctgggtttg
    77581 tccaccagac agcgttgcga tatcccgcat acgaaatcca gctagacctg tagcatccaa
    77641 cgcccactca attgcttcat gatcttgcgt gtccatgcca ctgaacagtt tttgataagg
    77701 gtatcgtccg taagatacta cttcttctac tgtcatacct gtagggtgtt cactagtttg
    77761 tgctaataat gcaatttttt ttgcaagttc ttttgaactg tatgtagaga tatcagagtt
    77821 attgacaatg atctttcctg atttcacagg tagtatacgg gtaaaacttt tcaataaagt
    77881 agattttcca cttccatttg gcccaatcaa actagtgatt ttcccttcag gaatcgtaac
    77941 agacatgttt tcaatgattt ttttgttttc gtagccggct gcaatgtcag ctgctcttaa
    78001 tctcgtcatt tcacccatcc tttcgctgaa aatcattctc aattatgacg ctgtttcaac
    78061 atttcgtcaa gcgctacttt catatctata gccaaatgaa gcggttgaca gcgttcgaaa
    78121 tatcaggaat aataaccaat gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa
    78181 ttaaaaacaa aagtattcac attatctagc atattgctgc tggctggagt actgagcgct
    78241 tgctccccct cttcagataa agaaacagct acaactgcca attcgacgac ttcatccaat
    78301 agtactcata cagtcacaga tacacttgga cataaagtag agattccaaa cgcgcccaaa
    78361 cggatcatcg gcagttactt agaagactat ctgattgcac taggggaaaa acctatcgcc
    78421 cagtggactg ttggcagtgg ttccattcaa gattatctgc aagatgattt aaaggatgtc
    78481 ccaacgattt cttatgacct tccttatgag aaagtgttaa gctttgagcc tgatttgctg
    78541 ttgatttctt cttccgctac tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg
    78601 ccaacttatg ttgtaaaaaa cggaacagat gtcacatggg aagaacaact aaaagatgtc
    78661 ggcaaagctt tggcaaaaga aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc
    78721 atcaagaaaa ctcgagaaga actagcagat aaaatcgaga acaagacagc tgcagtgttg
    78781 tgggtgacca acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta
    78841 tacgacgatc tggaatttgg tgttcctgat ctagtggaag aggtctccaa aaaagccact
    78901 tctgactggt ctgctgtttc ctctgaaaag ctagctgaat tagatgctga ctacatcatc
    78961 cttgtgaaca gtgacaaaga tgctgccatg ttcaatgaac caagctggaa gaatctaaaa
    79021 gcagtgacag acaatcatgt actgcaattc ggacctgaat ctagttggct atataacgga
    79081 cctattgcca gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg attttaacta
    79141 aaaaaagcat cttcacgatt agtcgtgaga tgcttttttt cttattaaca aactagacgc
    79201 atagatttta ttcttctttt gaagcaaccc tattttttac tctttcccat ttttgaaaac
    79261 gatgggcata tagattttct tgatccgtta tcccctcata ctcctctata agggcgaagt
    79321 ctgaaaaatc aatattccca atatacgtat cgccttcaaa cgtgctatcg atcaatgttc
    79381 gccagatccg accagtgtaa ggaagaagtt cttgaaaaat ccgactacca cccgaaacat
    79441 agatatcttc tgagatcgtt tgactatacg acaaaagttc atcgatcgaa tgcattactt
    79501 ccgcattttc tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata tggcgataag
    79561 gaagtgaaag atcccccatt ccttcatagg tctttcttcc catcactaaa attcgatcca
    79621 ttgtatgttc gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac aaaccatttt
    79681 tgccgatcag accattttta tcttgtgccc acatggaaat aaacatatta tccgctcctt
    79741 atgattttag atgttttgag ataaaaaaag tctcaattct ttgaccagca aggaattgag
    79801 aactttaaaa acattatttt gtttcacggt gcaaagtaac tttgcgttcg cgtgggcaat
    79861 atttttgttt ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt agatagttac
    79921 gttctttaca agaagtacac tctaaagtga tgtttacgcg catgtttttc cctcccattt
    79981 ttacaagaat cctctaagat gactctcaga cttgattatc ttaccatgat tttttctaga
    80041 ttgctagtgt ttttcacgat tttgttaagt aaaactactt gacgataaat acaacggttt
    80101 tacttctcaa acaaaatctt tttctttttg tataaatacc cttacacttg tttatataga
    80161 ttatgttttt taataaaggg ggatactaat atgccatgga acatgaagga ctttcctgca
    80221 tcaatgaaaa acttagataa attgacaaga aaaaaagcaa tcgacatcgc aaatgcatta
    80281 ttagatgaag ggtaccctga taaccgtgcg atcccaattg ccattgatca agcaaaaaaa
    80341 tggtatgaag atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa aaatccaagc
    80401 aagacggatg aacacgatac gaatcctcgt gctggaaaac tgcttgattc agatgtgatc
    80461 gtagaatatg aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa ggcaagcaac
    80521 cattttgata caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca aaacaaagaa
    80581 tcaactttgt tcatttataa aaaagatggg acaaaagaaa aagaaatcga ttattgaaca
    80641 tatgtaaagg ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga aacaatggtg
    80701 ttcctctgcc aactccgaac cataagtcat aatatctata aaacgggaag agctgttttt
    80761 tgaaattgtt tcttatcact cagtgttaaa cggtaggaga aaacctcttt tttattgata
    80821 tttgattttc atcaaacagt cacatagtcg attgcttctg cttgcggaat cgtttcatct
    80881 acaaagtaga ctttatacca tgtcaagaaa ctgaagattg tccagatttt tctttgctct
    80941 tctttacgtc cttcgtgatg gtcatctagc aacttcatga ttttttcttg atcaaaaaat
    81001 tcttttgcaa aatctgcttc gaataatgct ttgacttgtt cgtagccgtg ttcttctttc
    81061 aaccaagctt tgattggcac agggaatcct aatttcacac gattcgacca ttcttccgga
    81121 agatgtttgt tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt caataaaaac
    81181 ttacttggta tttgttgagc caatttcatg acttcgatat ccaataaagg tactcggact
    81241 tccaaagaac ttgccataga aagtttatct gcctttaaca agatatcttt tggcatccat
    81301 tgatgaagat cgacatattg catcttattg acttcatctt tgattccttc tgttttctcg
    81361 taatgaacag tcatgatttc atccacagat ggtgctgttt gatattttgg tgttaacact
    81421 tctgatgctt ctgattcttc gaacactcga gcatgaccga taaaatattc ttttgcagga
    81481 gcaagtgatt catataaatg gatccgtcca tggaagtttt tcatttttcc gattttacgt
    81541 cctaaattat agcgtgttcc ttttggtaat tttttcaatc cttgagcaat cacacggatg
    81601 aattttgaat tcgtatggaa tccatacgtt tggtatcccg caaacagttc atccgcccct
    81661 tctccagatt ggactacccg cacactttgt gaagcaagat ttgctaaaaa gtacaaaggt
    81721 acgcaagaag gatttgaatc aggttcatct aagtgatact ggatcaaagg gaatgctttg
    81781 aacgacatgt ctccgtcgat cactgctgct gtattatcta agtccaatag ttcggttagt
    81841 tttctggctt caatcgcttc attataggtt ttatcatcaa acccgattga gaaagaatga
    81901 tctggtttca atacagaggt tacataactg gaatcaaccc cgctggataa gaaagaacca
    81961 acttctacat cactgaccgt atgtgcttca atagaagctt gaacggtttc gtcgattttt
    82021 tcgatccatt cttgtcggga atgcgtttct tttgtttcaa aatctgcatc ccaatattgc
    82081 tgaatgtcta tttttccatc tttatacgta taataatggc cttcaggcaa acgatagaca
    82141 tctttgaaga atgtttcgcc gtttaatgga gaatattgga aagtcatata aggtttcaac
    82201 gcttctttgt tcaattcttt gttaaaatca ggatgaggca gaaaactttt gatttctgaa
    82261 ccaaacataa atgttccgtt catttctgca taataatatg gtttgatccc aaagtggtct
    82321 cttgcaccga ataactcatt cttttcatta tcccaaatag caaaagcaaa cattccgcgg
    82381 atcttttgca ataattctgt tccccattct tcgtaaccat gcaaaaggac ttctgtatct
    82441 gcatgtgttt gaaaaacgtg gccagctgca atcaattctt ctctcaaagg ctggtaatta
    82501 tagatttcgc cattaaaaat aatgatcttt gtccgatctt cattgtaaat aggctgagta
    82561 ccgccctcta agtcaataat acttaatctt ctgaatccta aagcaacatg tttatcgata
    82621 tattctcctg aactattagg tccacggtga acgattcgat ccatcattgt attaataatt
    82681 gtttttttgt tgtccttgtc gttaacaaaa cctacaatac cgcacattta actttcacat
    82741 cctaatataa aattactgtt tatttcattc atatgcatct tatcatagaa taaatagcgg
    82801 acgaatattt tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa gtttctgttt
    82861 ttacagaaaa taaagtccca atatctaatt taccatgttt tcttttttga ggctacctta
    82921 ttctcttcgt gttaaaaaaa tattagtgga tgctatttcc aaaaaaacca gcaaaaaaag
    82981 acacgaccta aa
//
LOCUS       DACJGB010000004        66966 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.004, whole genome shotgun sequence.
ACCESSION   DACJGB010000004 DACJGB010000000
VERSION     DACJGB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.004"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(501..1055)
                     /locus_tag="IYI34_000297"
     CDS             complement(501..1055)
                     /locus_tag="IYI34_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAR0558201.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(1181..3110)
                     /locus_tag="IYI34_000298"
                     /pseudo
     CDS             complement(1181..3110)
                     /locus_tag="IYI34_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(3416..4132)
                     /locus_tag="IYI34_000299"
     CDS             complement(3416..4132)
                     /locus_tag="IYI34_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAR0558202.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(4133..5956)
                     /gene="glpO"
                     /locus_tag="IYI34_000300"
     CDS             complement(4133..5956)
                     /gene="glpO"
                     /locus_tag="IYI34_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAR0558203.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5970..7466)
                     /gene="glpK"
                     /locus_tag="IYI34_000301"
     CDS             complement(5970..7466)
                     /gene="glpK"
                     /locus_tag="IYI34_000301"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAR0558204.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7602..9062)
                     /locus_tag="IYI34_000302"
     CDS             complement(7602..9062)
                     /locus_tag="IYI34_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0558205.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(9052..10347)
                     /locus_tag="IYI34_000303"
     CDS             complement(9052..10347)
                     /locus_tag="IYI34_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR0558206.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10679..11356)
                     /locus_tag="IYI34_000304"
     CDS             complement(10679..11356)
                     /locus_tag="IYI34_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAR0558207.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11386..12477)
                     /locus_tag="IYI34_000305"
     CDS             complement(11386..12477)
                     /locus_tag="IYI34_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAR0558208.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12578..13012)
                     /locus_tag="IYI34_000306"
     CDS             complement(12578..13012)
                     /locus_tag="IYI34_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0558209.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13596..15053)
                     /gene="ascB"
                     /locus_tag="IYI34_000307"
     CDS             complement(13596..15053)
                     /gene="ascB"
                     /locus_tag="IYI34_000307"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAR0558210.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(15050..16945)
                     /locus_tag="IYI34_000308"
     CDS             complement(15050..16945)
                     /locus_tag="IYI34_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0558211.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(17003..18778)
                     /locus_tag="IYI34_000309"
     CDS             complement(17003..18778)
                     /locus_tag="IYI34_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0558212.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18828..20324)
                     /locus_tag="IYI34_000310"
     CDS             complement(18828..20324)
                     /locus_tag="IYI34_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR0558213.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20518..22140)
                     /locus_tag="IYI34_000311"
     CDS             complement(20518..22140)
                     /locus_tag="IYI34_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAR0558214.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22468..23781)
                     /locus_tag="IYI34_000312"
     CDS             complement(22468..23781)
                     /locus_tag="IYI34_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAR0558215.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(24026..24250)
                     /locus_tag="IYI34_000313"
     CDS             complement(24026..24250)
                     /locus_tag="IYI34_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558216.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24270..24638)
                     /locus_tag="IYI34_000314"
     CDS             complement(24270..24638)
                     /locus_tag="IYI34_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAR0558217.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24834..25544)
                     /locus_tag="IYI34_000315"
     CDS             complement(24834..25544)
                     /locus_tag="IYI34_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558218.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25562..26059)
                     /locus_tag="IYI34_000316"
     CDS             complement(25562..26059)
                     /locus_tag="IYI34_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0558219.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(26096..26548)
                     /locus_tag="IYI34_000317"
     CDS             complement(26096..26548)
                     /locus_tag="IYI34_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0558220.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26683..27288)
                     /locus_tag="IYI34_000318"
     CDS             complement(26683..27288)
                     /locus_tag="IYI34_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAR0558221.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27738..28358)
                     /locus_tag="IYI34_000319"
     CDS             complement(27738..28358)
                     /locus_tag="IYI34_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0558222.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28360..29229)
                     /locus_tag="IYI34_000320"
     CDS             complement(28360..29229)
                     /locus_tag="IYI34_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAR0558223.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29252..29659)
                     /locus_tag="IYI34_000321"
     CDS             complement(29252..29659)
                     /locus_tag="IYI34_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAR0558224.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29678..30967)
                     /locus_tag="IYI34_000322"
     CDS             complement(29678..30967)
                     /locus_tag="IYI34_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0558225.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(30994..31296)
                     /locus_tag="IYI34_000323"
     CDS             complement(30994..31296)
                     /locus_tag="IYI34_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0558226.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31321..33414)
                     /locus_tag="IYI34_000324"
     CDS             complement(31321..33414)
                     /locus_tag="IYI34_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR0558227.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33903..34667)
                     /locus_tag="IYI34_000325"
     CDS             complement(33903..34667)
                     /locus_tag="IYI34_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAR0558228.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34778..35647)
                     /gene="mdcB"
                     /locus_tag="IYI34_000326"
     CDS             complement(34778..35647)
                     /gene="mdcB"
                     /locus_tag="IYI34_000326"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAR0558229.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35672..36748)
                     /locus_tag="IYI34_000327"
     CDS             complement(35672..36748)
                     /locus_tag="IYI34_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAR0558230.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36772..37365)
                     /locus_tag="IYI34_000328"
     CDS             complement(36772..37365)
                     /locus_tag="IYI34_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAR0558231.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37369..39045)
                     /gene="mdcD"
                     /locus_tag="IYI34_000329"
     CDS             complement(37369..39045)
                     /gene="mdcD"
                     /locus_tag="IYI34_000329"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAR0558232.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(39035..39334)
                     /locus_tag="IYI34_000330"
     CDS             complement(39035..39334)
                     /locus_tag="IYI34_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAR0558233.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39436..41082)
                     /gene="mdcA"
                     /locus_tag="IYI34_000331"
     CDS             complement(39436..41082)
                     /gene="mdcA"
                     /locus_tag="IYI34_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAR0558234.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(41089..42000)
                     /locus_tag="IYI34_000332"
     CDS             complement(41089..42000)
                     /locus_tag="IYI34_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAR0558235.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            42120..42977
                     /locus_tag="IYI34_000333"
     CDS             42120..42977
                     /locus_tag="IYI34_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR0558236.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43164..43526)
                     /locus_tag="IYI34_000334"
     CDS             complement(43164..43526)
                     /locus_tag="IYI34_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558237.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            43872..45311
                     /locus_tag="IYI34_000335"
     CDS             43872..45311
                     /locus_tag="IYI34_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0558238.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45318..47870)
                     /locus_tag="IYI34_000336"
     CDS             complement(45318..47870)
                     /locus_tag="IYI34_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAR0558239.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(47984..49315)
                     /locus_tag="IYI34_000337"
     CDS             complement(47984..49315)
                     /locus_tag="IYI34_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0558240.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49281..50759)
                     /locus_tag="IYI34_000338"
     CDS             complement(49281..50759)
                     /locus_tag="IYI34_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0558241.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(51064..52899)
                     /gene="tdc"
                     /locus_tag="IYI34_000339"
     CDS             complement(51064..52899)
                     /gene="tdc"
                     /locus_tag="IYI34_000339"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAR0558242.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            53369..54796
                     /locus_tag="IYI34_000340"
     CDS             53369..54796
                     /locus_tag="IYI34_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAR0558243.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(54888..55253)
                     /locus_tag="IYI34_000341"
     CDS             complement(54888..55253)
                     /locus_tag="IYI34_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAR0558244.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(55250..55615)
                     /locus_tag="IYI34_000342"
     CDS             complement(55250..55615)
                     /locus_tag="IYI34_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAR0558245.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55807..56055)
                     /locus_tag="IYI34_000343"
     CDS             complement(55807..56055)
                     /locus_tag="IYI34_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558246.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            56054..56182
                     /locus_tag="IYI34_000344"
     CDS             56054..56182
                     /locus_tag="IYI34_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAR0558247.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            56239..57933
                     /locus_tag="IYI34_000345"
     CDS             56239..57933
                     /locus_tag="IYI34_000345"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAR0558248.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            57937..58056
                     /locus_tag="IYI34_000346"
                     /pseudo
     CDS             57937..58056
                     /locus_tag="IYI34_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            58138..58329
                     /locus_tag="IYI34_000347"
     CDS             58138..58329
                     /locus_tag="IYI34_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558249.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            58707..59840
                     /locus_tag="IYI34_000348"
     CDS             58707..59840
                     /locus_tag="IYI34_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAR0558250.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            59871..60191
                     /locus_tag="IYI34_000349"
     CDS             59871..60191
                     /locus_tag="IYI34_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558251.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(60220..60684)
                     /locus_tag="IYI34_000350"
     CDS             complement(60220..60684)
                     /locus_tag="IYI34_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0558252.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(60815..62068)
                     /locus_tag="IYI34_000351"
     CDS             complement(60815..62068)
                     /locus_tag="IYI34_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0558253.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLPIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(62097..62414)
                     /locus_tag="IYI34_000352"
     CDS             complement(62097..62414)
                     /locus_tag="IYI34_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAR0558254.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(62440..62760)
                     /locus_tag="IYI34_000353"
     CDS             complement(62440..62760)
                     /locus_tag="IYI34_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0558255.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(63224..64639)
                     /locus_tag="IYI34_000354"
     CDS             complement(63224..64639)
                     /locus_tag="IYI34_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR0558256.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(64656..65972)
                     /locus_tag="IYI34_000355"
     CDS             complement(64656..65972)
                     /locus_tag="IYI34_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0558257.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(66122..66820)
                     /locus_tag="IYI34_000356"
     CDS             complement(66122..66820)
                     /locus_tag="IYI34_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAR0558258.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT        19674 a        14230 c        11275 g        21787 t
ORIGIN      
        1 tttgacctaa gtattagaga aaaaaacggg aaaatagctc ctcatatttt gccgtttttt
       61 gagctaatac cgaaggtcag ctgattgaac accgtttaag gaagagattg tgaaaaagtc
      121 gcttagtttc gagtattaag aaaaattttg tgaaaatgga tttctacatt tttgcataaa
      181 tttggcttaa taccgagaaa ctggcttttg aataccgttt aaggaagagg ttgtaaaatc
      241 agcggtttga cctgagtatt agagaaacaa acggaaaaat agctgctcat attttgccgt
      301 tttttgagct aataccgaag gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc
      361 taccatttaa ctccgaatca caagaaataa tttcaaaaac aacttctcct gtttttggtt
      421 cggttggctt attatcgaga agtagctact gggagtctat ttatcaaaaa aaggaatgcg
      481 acataggtca cacccctttt ttattttctt tgaaacatct ttcggtactc gacagcatac
      541 aagacaaaag attgtaaaag accgtaagtg atgacggcaa aaatgactct cacccacagc
      601 gttggtaatt ctgggaaaag cagcaaaagg atcatgccaa catagatgac agcagtcgct
      661 actttgccat accacttcgc tccacttaat ttgatatttt tagatagccc gattaatcca
      721 gctacaaaca taaagccttc ttttatcaag aatagaccaa acaacagcca aagccacggt
      781 cgataccatg ccatagacaa aatcagaaaa agttgtgtca atttatctgc aaacggatca
      841 agcaccttcc ctaagtctgt tacctgatta tatctccgag caatgacccc gtctagataa
      901 tcagacaaac ctgagacaac aatgaccgct cctgctatat aaaatgactg tctcagcaca
      961 aaataaagat aaatcggcag caaaaaaata cggaatatcg ataaaagatt cggaatcgtt
     1021 tgtatttctt tacgccagtc tgtgcttaat ttcattttat cgccctacct cctcagcact
     1081 cttttctttt tattttagct tacacgggga ataaaaccaa tgatttcact gtttttattt
     1141 ttctttacag acactgatat tatgagctaa tttgagataa tcacttgctg ttttcatcgt
     1201 ttactcttct ttcttcctgt cgtttgcgca tttcttttac tagtacttta tgagcttttt
     1261 ttgatcctcg ctctaggtca cgctgccgtt tacgtgtttt ataatcgata aacagactgt
     1321 ctaaatcgta acctgtcggg tacagatctt ctgctgaacg caccagacga acacgctgac
     1381 gaggcacgtg gattattttt ttgtctaaat atacatcaat cgtatcttca cctatatctt
     1441 cataaactaa cgcttctttt tggtattcat tgacaaatat ccgatcacct tttgcaaaga
     1501 gctgacgttc ttttttattc tccttcctat ttgtaactgc tgtcttagga agaaattctt
     1561 ttttctgtgt ccgataggct ttttccgcca agtaacgctc tgcttgatgg atcaatttca
     1621 tcgacatatc cattttatga gcaatccaca aagcttggct ttctcctgtt tcttccaacc
     1681 cgcaactgat atttaggacg aagtgcttcc cgatcgaaag ccatagcagc cggcacaaaa
     1741 tcttcatgat ctccggcgaa tttcttgatt tctccataat gggtcgtggc aatgatcaat
     1801 gccccttgct catacataga ttccatgatt gcgatcgcta gtgcagcccc ttcatttggc
     1861 tctgttccac tgccaatctc atctaaaagt accaacgtat tcctttttat ctttttcaaa
     1921 atagcagcga tgttttgcat atgaccagaa aatgtactca acgcattttc taaattttgg
     1981 tggtcaccga catcgacgaa aatctcatca aagacagcaa gctctgtccc ttcttttgcc
     2041 ggaacttgca taccgaacat cgccatcaaa gtcaataaac cgactgtctt caacacgact
     2101 gttttgcctc cggcatttgc tccggtgata atcaagcctc gatagtcttt accgatttca
     2161 acatccaatg gtaccgcatg ctcaggtaaa aatggatgtc tgccttgctt gataatgata
     2221 tgttccgatt tattgattct aggtgtgctt ccattgattt ctcggctaaa ttttgcccga
     2281 gcaaagatga tatctaacac cgtgatcgtt tcgatcaata aatcgatcac ttcttcgttt
     2341 tctgcgatag caccagtcaa tgccgccagg acttgatatt cttcactgat ttcttcagct
     2401 tttaacaact gatagtgttc attcaattta gaaatcacag ttggttcaat aaaaaccgtc
     2461 gttcttttat ttgattcatc gataatcgtt ccatctattt tatttttata gctggctttg
     2521 ataggaatcg tatagtattc gcctttttga acaatcattg cttcttggat catttctttg
     2581 tttttcggat gacgcaagaa tttcagtaat tttgactgga tttctttttc gattagttgt
     2641 aattgtttgc gtactttacg caaattacgt gacgcgtcgt cactgacttt attatttttt
     2701 attttctggt caatcaactc ttctacattt atcaaatcag gaagatgttt gctataagca
     2761 aacaacaagg gtgtctggta ttgattctta tcaaaaaact ttgtgatcat ccgactgctt
     2821 ctaagaaaat ccgcatattc gatcaaatcg gttggctgca gaatcaaccc ttttttgact
     2881 tgttctgtca gtgcatcgat cctcggcaac cccataaatg gaacatgctg actgctttct
     2941 aagatcaagc gtgcttctct tgtttcttct tgacgatcca agacagtttg taggtttgtc
     3001 gagaccgtca tatcgctgat tcgttttttg ctatagtttc caattgctct tgcttggatt
     3061 tcctttttga tccgatcaaa ttgtaattga ctaatcattt cgttgtccat attctctcct
     3121 tttcttgaag agatacctgt cccaaaataa ataagggcct agagataaat aaaaggatag
     3181 aacacagctt aaaaaagtca tgtcctatcc ttattagaat acaaagatat tgttttcctg
     3241 acaggtaccg tttcataagc acaccaaata aactttgttt attggctatt cgacttactg
     3301 attagatacc agctacactc acaaaacata ccccattctt ctaggtctta tttattttcc
     3361 acttaactat agcaagacac attttggttg tcaaacttta gtatgtttta tagatttaca
     3421 ccattaatga atagacacct accgcgataa ttgctccgat cattggacct gcgattggta
     3481 cccatgaata tgcccaatct gaagttcctt tattagctat tggcaatacc gcatgtgcta
     3541 atcgaggacc aaagtcccga gcagggttga ttgcatatcc tgttggccct cctaatgaaa
     3601 gaccgatggc taagatcaat ccacctacag cgaatacgtt cgttccaggt gcaaattcgt
     3661 tttgtccaaa agctagcaac ccaagaacta atacaaatgt accaattaat tctgtgatca
     3721 catttgctgg ataattccgt atagcaggac ctgttgcaaa cgttcctaaa atcgcgcttt
     3781 catctttcgt tgcattccaa tgtggcaaat aagaaagcca aacaagaatg gcccctgcaa
     3841 aagctcctaa aacttgcgca atgatgaaag gaacgacaag attccaacta attgcccctg
     3901 tcatcgccat agctagagag actgcaggat tcaaatgagc tgggcccatg aatccagaaa
     3961 tatagacagc cattgttaca gcgagtcccc aaccaaatgc gatgacaatc cagccagaag
     4021 cctgtgcttt acttttgttt aaattcacag ccgcacacac accatctcca agcaaaacta
     4081 ggatcagtgt cccaataaac tcccccatga tttgagtcat atcactgttc atttatttct
     4141 ccccctttag ttcccgcaga tctgattctg caatcaatgc ttctaaacgt ttagtctgtt
     4201 gcgccttctc ctcttctgtc cattcaaaat atccagcaat tgcatcgatc acaggttgct
     4261 tgatctcatc aagctgatct cgttcaaaca acagatgatt tgtacgacga atgagataat
     4321 cgcctggggc caaaaccatt tcttcttcta aaccataacg taaccgagct gactcagcga
     4381 gactcaaacc aggataaggt gccatttctt ttgctaattc aaagatacgt cgggcattag
     4441 tcccgtaaaa atcagcgata taagtagcat cttcttcctc taaaccggct gctactcctt
     4501 cttttgctaa ttctgtcact gtttcttcta atttagttgg atcgaagttt ccacctgaaa
     4561 tctgatattt tttagagtcg atttctttcg tctctattcc gtattcttct tttaataatt
     4621 ggcgtatcaa tcgcaaagct ccttctgcca tcttacggta atcggtgatt ttcccaccag
     4681 ataaagtcag caaaccatct ggttctcttt ctaaagaact acctctggaa atcgtagaag
     4741 gtgctttttc atcacggctg ttttccaaat ggttcaacac gtcttctact tcagcacgag
     4801 aaactttgtt ttccttatat tcacttactg tatcgacaac tttattgaag ctcttgtctg
     4861 aaatcgatcc attatctcca ccattgtaat cagaaccaga attaccaatc aacagtggac
     4921 gaagtcctgc ccagcttgct tcgatatcat tcaatgtgat atttgcttct ggatagcgat
     4981 gattgatcac atccaataga taatctacat cttcttgtgt gactttcgga tccgtaaaat
     5041 ctccttggta atccgtatct gtcgtaccaa agtaagtctt gttttctctt ggaatagcaa
     5101 aaaccatccg cccatcttgc tttcctgtat cgaagtatgt gggttgcggt acaggcagtt
     5161 ttttcgcatc tacaactaaa tggatccctt tagttggacg cattttagga gagactggac
     5221 gcgtaaaatt caagtttcgt actttatcta cccaaggacc actcgtattg atcactaatt
     5281 ttgctttgat ttcgatcact tcatctgtca gcaggtcacg agctttgacg ccaacgattt
     5341 gatcgccatc atataaaaag ccaaccgctt tcattttact tactagataa gccccatctt
     5401 cggcagcctt tttgatatta tcaatcacta aacgggcatc attgttgcgg aaatctagat
     5461 agacacccgc gccttttagc ccttcttttt tcaaaaacgg ttctctttcc aatacttctt
     5521 ctggtgtcag cgtataattc tcatatttgg ttcctgtcac atttgccagt ttgtcataaa
     5581 ggtccattgc tactttgacc gagaacatat tgaaggttgt tgctccttcg ccctcataga
     5641 ttggcaaaag cattggatct ggttttggga tgtgtggggc aatactttgt acgactgcac
     5701 gttcaccaac tgtgtcagct actacttcca catcaaatgt cttcagataa cgaataccgc
     5761 catgaacaag tttggtcgag cgagaggaag tcccttctgc aaaatcctgc atttcgatca
     5821 atcctgtttt gattcctgat gctgctgact ggactgccac accggcacca gtgattccgc
     5881 caccgatgat caacagatcc aattcttctt gctgcatttt ttgaatgcta tcttgtcttg
     5941 tcttgtttga aaacatatgg atttcctcct tattcaaatg cttgggtagc ttttactgct
     6001 tttttccaac cgttgtaaag tttttctcgg cgctcttcct ccatttgaac ttcaaatagc
     6061 ttgccagctt cataaaattc gcggatctcg tctgtatctt tccaataacc gacagctaat
     6121 cctgctaaaa atgctgcccc tagcgccgtt gtttccaagt tttctgctcg ttggatcggt
     6181 acattcaaga tatctgtttg gaattgcatc agatattcat tgtttgctgc accgccatct
     6241 actttcaata caggaatctt gattccagtg tcatcttgca tcgtatcgat gatatctctc
     6301 acttgataag caatcgactg taaagtagct ttaatgatat cttcttttgt cgtaccacgt
     6361 gtcaagccaa acatagcccc tcgtgctgcc tgatcccaat aaggagcacc tagcccaacg
     6421 aatgcaggaa caacataaac ctcatcttcg cttgtagagc gtttcgctgc gtcttctgaa
     6481 tcgctagctt tttgcaacat ttgaagtcca tctctcagcc attgaatcga agaaccagcc
     6541 acaaaaatac ttccttctaa agcatagtaa acctttccat ttatgccgta accaattgtc
     6601 gtcaataaat tatttttgga taattgcggt ttttcaccgg tattcatgac aataaatgaa
     6661 cctgtaccat aagtattctt gaccatgcct ggttcgaatg ccatttgtcc gaataatgcg
     6721 gcttgctggt ctcctgccat ccctgaaatt ggtgcttcgc ttccataaaa atgataacct
     6781 tgcgtataac cataaacttc tgagttagaa gtgacttttg gtaacatcac tcgaggaata
     6841 tttagtaaat ctaaaatctc ctgatcccaa tctagctgat ggatattgaa caacatcgtc
     6901 cgactggcat tcgagtaatc agtaacatgt gcttgtccat cggtcagttt ccatactaac
     6961 caagaatcga tcgttccaaa catcaattcg cctttttcag ctcgttcttg cgcgccttct
     7021 acatgatcaa gaatccagcg tatcttcgtt gctgagaaat aggcatcgat cactaaccct
     7081 gttttttcgt ggaaaaattc ttgatagcct tcatttttta agttatctgc aattccagct
     7141 gactgtctgg attgccaaac aactgcatga tagattggac gtcctgtatc tttttcccat
     7201 attactgttg tttcacgctg gttcgttatg ccaatcccag caatctgatt tggtttgatc
     7261 cctgattcga tgaacgctcc ggcaataact gactgtacag aattccagat ttcatttgca
     7321 tcatgttcca cccaactttc ttgcgggaaa tattgcgtga actctttttg agaactgccg
     7381 atgtgtcttc ctttcttgtc aaagataatt gctcgtgagc tagttgtacc ttggtcaatc
     7441 gccatgatat acgttgattc tgtcattgtg attccctcca ttatatgcaa acgctttctt
     7501 taactactct tatcatacta ctgttttaaa aaaagaaact gtcaaaattt tattcttttt
     7561 ttcttttttt gacagttttt tattatgaag ctatttttta atcaattctt ttcttttttc
     7621 agcgtctcta ttcttctttt caatcgaata atgtcgtaag gtgacgcaaa ttctgacaga
     7681 taatattcac actgtttttt gtttctctgg caaaatgtga tcaccaaatc atattcttta
     7741 ccttcttcat aacttccaat ctcaataccg ccgattcccc tcagttctct gatcagatat
     7801 tgttgaaaag caatacgata aatcggcaga ctttctaaat cgattccaat cactactggt
     7861 tttgcaatat aaaaatcgat catcaaaaga acattggcat actggatcat caggtaacta
     7921 agctcactat cttcagctct tttcctcggc aattgctgca ccgctgtttc ctgtaatttc
     7981 gccagtaact gtgttaagga atgcccgagc attttctgct gctgttccaa aagccgatca
     8041 cgctcataga cttcgatctt tccaacaaag aaaaaccatt cattgttgat ctgggaaatt
     8101 tggtagccta ctgccttttc ttctttgatt ttcaaacgac gaggacgata atgccataac
     8161 atcgtttccc gaatgtaagt atctaaaaca gcggttggta actttttgct ccgtgtcaaa
     8221 tcatactgat aaaatatttc ttcgtctact atcgaaaaag aaacaaaaaa gctgtaaaac
     8281 atcattgttt cgtatacatt cagttctgct gctgttcgac taagatgcat cacgatgatt
     8341 ggatcgattg cttgataaag ccggtccgac tgatagagta atttcttttc tttcaaagtt
     8401 gcgtacgtac ttttttcatt caatagtctt tttttcgtaa tcccgagcca gcaagcaatc
     8461 tgtgcttctg ctgatgccgt aaacgttgtt tctaatacac ggtccaatcc tagaatgaac
     8521 ggtctttttt ctggtgtatt ttgcaaaaaa agcggtcgct gatcttccgg gatatattgg
     8581 aatagttcat aataaaaata tcgtatctgt agctcttctc cgtataattg accgtttttg
     8641 atttgtaatc caaattcctc taagagctgg ttcaattctt tgatctttcg aaaaacagaa
     8701 gactcactga tattaaacgc tgtcgctaat cgaacgatgg atacttgctg atgttctaat
     8761 aaatagacga gcatcttaaa cttcaagctt tcctgcaata aaaacaatag aattttttcc
     8821 aaacttacat cttcgtccaa cacaagcagc gcctgatact catttcgctg tacttctact
     8881 tttttttcca tcattcggcc aatcaattca atatcttcca aatattgatc aaacgaggct
     8941 tttgacagtt ggacatgctc tctcaaacca ttgatagagg ctgttccccc ttgcagaaca
     9001 aggtttcgca aaatcaggat ctgtaactgc tcttttcttt ctaataatgc ttcaatcttc
     9061 ataggcagta ttcgccacct ttgaccacac catatctggc acacggtatt ctggctggaa
     9121 aaatagctca tgttcttcta actgttcaaa cgtccatcct tcttctactg caattgtcag
     9181 ttggtcaaga aggtggaaca tctctttttt gctttggagc tgtgctccaa gaatcttttt
     9241 ttcagaatca aatagaaatt tgagtttaac tttgctttga tttattgctg taagcttgta
     9301 gtctacgtat cctgtttcaa ttttttgggg ataaaaaatg ctctcttctt ctgtcaaacc
     9361 tacgcttcct aagaaatagc caaaccactg attgccgact ggacgatagg ttccctgatc
     9421 ctttgtctgt gagccactaa ttgtcttgct gacatttcgt gctgtacgga cagcgttatt
     9481 tactagagaa acatacattt tttcatttgt tggacgaaaa gttacttgga tcgcatcgcc
     9541 aatcgcataa acgtcttttt gagacgtttg caagtagtca tctacccaga tcgttccatc
     9601 atcattcaat gttaattgtt cttcccacat ctgattgtcg ggacgagaat gattagccag
     9661 caaaacaaga tcatagttct cctttctttt ttccgtctct acaatagcgg tttcattttc
     9721 ggtcaaagac acaacttgtt cttctaaaaa aagtgaaagc ccttgatttt tcatttcctt
     9781 tactaacggt tctgtcattt caggatctag ataacgaaaa agaattgact ttcggctttc
     9841 gtaaagatgg atttgttttt ttcttgatgc caacccttca gctatttcga ttcctgcttg
     9901 tcctgcacca ataattgcaa ttttctttgc attcatgatc ttttgctgaa gagccgttac
     9961 gttttgtatc tttttaactg ggtgaataag cgttgattct gcagaaatat ttcgaaactt
    10021 ttgatttgaa ccagtcgcta gaatcaaacg atcaaaatcc aatttttttc ctcctgctaa
    10081 aacgactttt ttttctttca atcctattac tgtcttttct gtgtaaacat ggattcgata
    10141 gcggtcagtc agttcttctt tcgtgatcca agaaagttgt ttttcactgg taacctttcc
    10201 ttttaatatc aaattgaacc cgctgggaat ccatcctaac ttcgattgct tttcaatcaa
    10261 atatatttca cttgttggca tcaattggcg aagataaata gccgcatgaa tccctgcaaa
    10321 cgacccgccg acaattacaa tgttcatctg cattcctctt tcttataagg tgttctactg
    10381 ctaacttctc aatcagaaaa cacagtattc ataactcgct ttcaacctta tgatacatag
    10441 cgatgcagca gaatcaaccc tctccatcat accgtgttct aggtctgctt ctcaataatg
    10501 tactagtgga cctaattgaa gagtatcatt tctgaagaga tgtttcaaaa aatgtactct
    10561 tataaaagaa aaaaactcgc cttttacgta cctctgtcca aataggaaac agaaaagaac
    10621 gaaaagacga gttttagcgt gtgtaatgta atagttagaa tagacacatc atttattttt
    10681 aattctttaa tgcttcaaca actgcttcgc ctaattgatg agcagattga ggattttgcc
    10741 ctgtaagaag ccgcccatca atttgaacca cttctgaaaa agctgctcca gactgatagt
    10801 ttgctccttt ttctttcaaa gcatcttctg ctaagaatgg tacttcattc gttgtaccat
    10861 ttgcttcctc ttcttcatta ctgaacccag tcactgtacg tccagctaaa atagattttc
    10921 cagactcatc tgtaactggc aacaatccta caacaccgtg acaaacagct gagaccagcc
    10981 cttcatttcg atagatttgt gaagcgactt tcccaagatc tattgcttct ggaaaatccc
    11041 acattgttcc gtggccacca gcataataaa ccgctgcata atcatttgca tccacatcat
    11101 caggacgtaa ggtatttccc aaggcccgat ttctgaaatc ttcatcttca taataggccc
    11161 aatcgatttc atccatattc aaaacacttt gcggttctaa aggtacatat ccgcctttag
    11221 gactgacaaa atctgcatcg atcccatttt ttactaatac atcataaaaa tgagtcagtt
    11281 cacttaacca caaacctgta ggacgctcga tcttttcgta cttttctaca tttgttaata
    11341 cgatcaatac tttcattttc ccatcctctt tctataaaaa ttgcgttatt gataacgtgc
    11401 tttcaagaaa ttatataatg ggcttggcaa ataaagctct ttatatgctt cttcaaaagg
    11461 tttcgttaca aattcatcct cacggttttc caataatttc tctgcccagt caggatcgac
    11521 gatcatttgc tgtccaattg ctgcaagttc tgcatatttc aatacctgct ctacatcttc
    11581 gcgattgcgg atcccaccga caccaactaa agggatcttt ccttgaatcg tgttatggac
    11641 cacttcaaga atcgatttat cgttgtattt atcagaacga gctattcgat catacatatt
    11701 taatgaaaca tgcagataat ctagttttga ctcacgtaat cgttcaagaa gccagatcgt
    11761 gtcttcaaaa cggatacctg gtgtttcata ttcttctggt gagaaacgat accctacgat
    11821 gaatggtttt ttagcatatt gttcaaccgc tgagaaaacc gttgttaata attcttcaat
    11881 aaaacgataa cgattttcca agctaccgcc ccattgatcc tctcgtctat tcgaatgcgg
    11941 agagaaaaat tgttggatca aatacgtatt tgctccatgc aactcgattc catcaaatcc
    12001 tgattcaatc gctctacgtg tggctttgcc aaatgcttgg attgtttcaa cgatttcttc
    12061 ttcactcatt gctcgtggtg tttccgcatc aggacgtttt gctgcaactg cacttgcaga
    12121 tacaggctgt tctccagata aagttgctcg acttgtcatt cgtccaccgt ggaaaatctg
    12181 aataatcgct tttgcgcctt ctccttggat tgctttcgct agctcactta atcgaggaat
    12241 catttcatca tgagcgatgc tgagttcacc tggccagcct tttccgatat cttggacatt
    12301 tgcagcacca gtaataacag caccaagacc tttagaacgt tgtttataat aagtgatctc
    12361 atcttcggtg atcactccat tgaaaaagct gagttgtgtc gtcataggcg acagcatcaa
    12421 tcggttcttc aattgaactc ctgaaggtaa agtaattgat tgatcaaatt gtgacattgt
    12481 cattcctccc ataattcgct ctgattaacc tatcgagaat catctatcat taaaccactc
    12541 gattagttgt acatgcaact aatttgctta aaaaatttta ttttttttgt aacttatctg
    12601 ttgcatcatt cattgtctgg gctaattctt tacttgcttc atttcccaaa actgctttgt
    12661 atgactgatg aagttcttcc cagctaccat cgacttcctt agcaaattgg cgtccttgtt
    12721 ctgtaagata aactaatgac aagcgacctt cgctaactgt tttgacgtat cctttatatt
    12781 ctaatttttc aacaaaacga gcaatcgttg atggggcgga atagagcatt tgtgcaatat
    12841 ctttttgtgg tattccatct ttttctttta atatcaataa aataaaacca taagttggtg
    12901 ccagacctgt ccttctaaac gcttcatcag ctaatttatt caacatgcga tctagctttt
    12961 tcacgctaaa gaaaagacat tcatctaacg ctttttcaat tggcttttcc atttcatccc
    13021 ctccattggc gtacatatca tagtatgatt tttatttttt gtcaaaaaac acggaataat
    13081 gtccgcctcc tctgtctaga tttgaaaaaa agaagtagcc atcttcaaag aatttcggct
    13141 tgttttcgac aaaactcctt ctggaacacc gtgaatcaga ggttgtgaaa aagtcgttta
    13201 gttttgagta tgaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg
    13261 cttaataccg aaaaaatggc ttttgaacac cgtttatatc agaggttgtg aaaaagccgt
    13321 cctgcatcaa gtaataagaa cagccaaaca aaaatagttc ctcatatttt gtcgtttttt
    13381 gagctaatgc cgatggtcag ctgattgtac accgtttatt gagaggtcgt gagcctgccg
    13441 ttgaactccg agtcacaagg agcaatttca aaaaacagct tctcatgttt tatgactatg
    13501 gtgacttgtg atcgaggagt tggcaggaga atactgttta ttcgtaaaga aggggctgtg
    13561 acaagacttt tgtcacagtc ccttatttcc ttttattata ggatttcgcc atttgattgg
    13621 atgacttgtc tgtaccaatc aaatgatttt ttctttaacc gtttctttgt gccattccct
    13681 tcgtcatctt gatcaacata gatatatcca taacgttttg acatttgtga agtagaagcg
    13741 ctgacgatat caatgcagcc ccacgacgta taaccaaata aatctactcc atctttgata
    13801 gcttccttca tctgctcaat atgctgcttc aaatagtcaa tgcggtaatc gtcttgaacg
    13861 gttccatctt cttctacttt gtctatagct ccgagtccgt tttcagctat gaacagtggt
    13921 ttttgatacc tatcatataa ttgattcaaa gcaattctca aacccttagg atcaatctgc
    13981 cagccccatt cacttgatgg aagatacgga tttttaattc ctccagttat taagtttcct
    14041 tggacccctt cccaattttc tggatgagca ctgactgcac tagacatgta gtagctaaaa
    14101 gaaacaaaat caacagggta ggctgctaag atttcttcat cacctacttc tatttttaag
    14161 ttgacacctt tttcttcaaa cattcgttgc gtatacgctg gataatatcc tcttgcttga
    14221 acatcactaa agaacaaaga agttctttgc tgctcaattg ttttttgaat atcatccgga
    14281 tcacaagaat acggaacaag aagctgatag ctgatcatac aaccgacttg tgaacctttg
    14341 ataatttcat gacaagcttt tgtagctaaa gcacttgcta taaactgatg gtgtgcacct
    14401 tggtatttcg cttgttccaa atggggatgg ttttcttcaa ttactcctac gctaacaaaa
    14461 gggtgatgtt ttacacagtt gatctcattg aaagtcatcc aatattttac tttattttta
    14521 tagcgtttga atactgtttc agcgaaacga acgtaaaact gaataagctt tcgattaggc
    14581 catccaccat attctgtaac caaatataac ggcatttcat aatgggaaag agaaacgact
    14641 ggctcaatcc catattttag acattcgtca aacacttgat cataaaaaaa taatcccgct
    14701 tcattgggtg tttcttcatc accatgagga aaaattcttg tccatgcgat agacatccga
    14761 tagcatttaa aacccatttc agcaaataaa cggatgtctt ctttaaagtg atgatagaaa
    14821 tcatttccgt gtcttttagg ataataatac ttgctgttgg gatcctgcgc cgcttttact
    14881 tgctgatgcg tcatcatatg ccattgatct gtccattttt ctttaggtaa atcgctgtcg
    14941 aatgtataca catcagacac agataatcct ttttcatcta catcccatgc accttcacat
    15001 tgattggccg ctgtcgctcc gccccataaa aatccttctg gaaatttttt catatcgatc
    15061 cccctaaact tgctagtttt cttgtttgat tatttttctt atgactagta atgatatttt
    15121 tgaatctact actatttgta acaataacca ttgtggtcgg atcatatccc tctttgatta
    15181 attcttgcaa atctacttcc atcaataaat cgcctttacg tacttttttt ccatcagata
    15241 ctgcactttg aaagtatttg ccatttaaat tcactgtatc aataccgata tggatcagta
    15301 tctctgctcc ttcttcactg actaccccaa ttgcatgttt cgttggaaaa actgttgaaa
    15361 cttccccgtt tacagaactg tagatttttc cgtccgttgg aatgatcgca aacccttctc
    15421 ctaaagatcc actagcaaat gttggatcgt ttacttcaga taaatcaatt atttcacctg
    15481 ctgcaggagt acttaacact atatcctttg aggattcttc ttcgatttca tccttgtagc
    15541 cgaaaagaaa agttagcata caggaactga tcatcgctaa gaaataacta gcaactagcc
    15601 aaacaaatcc ttcaccaata taaatcggga aagacaaaat acttcttgta gcaactgcca
    15661 aagcaccggc tcctgcggca ccagctactc caccaaatat agctccacat atacaagcaa
    15721 tgtaaaatgg tcgtttatat ttaagagtca caccataaac agcaggttca gtgattccaa
    15781 ataatgctgt gataaatgca gataaagata aactttttac tttcttattt ttcgttttca
    15841 aaaatacccc aagtactgct cctgcttgcg cagctacggc agtcatacat gctggaccta
    15901 gcgtatcacg accgtagaaa gaaagattgt tggtcattac tggtacaagt ccccaatgta
    15961 acccaaaaat cactaacact tgccaaaatc cgccgataac agcaccagat aaaacagggc
    16021 taaagttata aacagctaaa tatccagctg caatcagctg acttgcataa tctgcaattg
    16081 gacctacaac taataaaata attgggacca tcacgatgat acaaatgaat ggtgtcaata
    16141 aatttcgaat actttcgtgc aagcttttgc gaatcttagg ctcaatataa gaaagaaccc
    16201 atacagctaa aattgctgga ataaccgtac ttgtatacct tgctagaatc acgggcatcc
    16261 ctagaaatcg caaggttatg ctatcactat atgcactgac aatcgtagga taaacgagag
    16321 ctgctgcaat aaccatagcg atgaaacgat ctgtattgaa cttttttgca gcagtatatg
    16381 ccaaaaatat aggcaagaaa tagaaaaatg catcagctgc tgcattcaaa atgatataag
    16441 ttcctgattc agtagttaat aaattcaaac tcgtacataa ggcgagtact cctttgatca
    16501 aaccacttcc tgccaagacg cctaaagtcg gggcaaaaac agcggcgatc atgtctataa
    16561 aagagtttaa aatgcctttt ttctcagttc ctctctttcc actagatgca tcatcttcta
    16621 gtttcatttg tttggataat acgtcataca cttcatttac tgcatttcct actacgactt
    16681 gaaacattcc tccagcttcg actacagtga tcacaccttc tagttgctct accttctcct
    16741 tatctgcctt cttattatct tttagggtaa agcgtaacct tgttgcacaa tggtaaacat
    16801 tgctgacatt ttcttcgcct ccgacaaatc ggacaatatc tttcgctaat tgtgaatagt
    16861 ccatttttac ttcccccttg ttaattaatg ccgtagcaat ccttcttaca agataagtat
    16921 agttcttaga aaacggtttc tccattcaat tagtacccct tctaatagcg aacgaaactg
    16981 tacaatagtg tgtatggttt acttagccga gcaaatcact cactgcttga aagaaaacaa
    17041 gtatatcgtt tctcttatag tcaaaggaga caaaaaaaac acgcttgatc ttcctttgct
    17101 gatgtataag tggtatctca agctgatact ctgtgatttt tgtttctact tggcttttga
    17161 taagacagca caacaaagtc tcggcattta acggaagcat aactaggtca gaactaggag
    17221 agattagttg ctgaatggaa tctatctttt tatggatttt tatttgattt tcttctttgt
    17281 agtaatgatc aagcagtttt gttttctttt tttcttcaac tatggtcagc ttttcacgca
    17341 aaatctgata aatctcccaa gattcaaact catctagaac actaggtaga taaatcaaat
    17401 catcccactc aaaaataagc tcttgctcag tagtcaaaat tgcatcatat tccgttattt
    17461 tgtttgattg atttttgaaa agatacactg gttctatccg gcattctttg atccacatgt
    17521 tcatactttg ttgaatctgt ttttttagtg tattcaaaat actaattggc tggttactaa
    17581 caatcagtat ctccccttgt tttcgaaaag gattcaacgc ttcggcaaga tacaaaacaa
    17641 aataagcgct ttcagctaaa ttgggacgta attgaaaatg tattgggaag aaacgccgaa
    17701 ttaaataaat ttctaaagga taacgggaga tgatcttttg tacaaaatga ttcatggtat
    17761 tatgtccggc atccatcctc gttttcattt gtttgatatg ggttagcaaa aattctgttt
    17821 catggaacaa taacgaaaca ggaagatcta aaaaacgaat aatcgtttgc tcaaatgctc
    17881 ttagattctt ctctatttct ttgttttttt catcattaat catcaaataa tttaactcta
    17941 atagtcgatt ttcaattgct tgtttttctt gattggaaaa agaatagggt agttctgatg
    18001 ataacttttt tgtgagggtc tctaccatcg gaataaataa tgtatacctg tttgtttttt
    18061 ctaaaagttc ttctcttcga agaatggtca aaacgagata ccgagaaaat cgtatgaagt
    18121 cctctcctga aacaatataa ttatatgata taagaacctc ttttaatgtc ttttggacag
    18181 tcggcatgat attggaaaaa attgtactga tttgttgagg aaaccttgcg tgttggatca
    18241 ccgtttctgt tccaacaaga gataagaaaa ttctttttga attttcttca gcaatgattt
    18301 ttagtccttt tgtcgacgaa acttccagtt ctaatttagg attgcgctcg atccaacgca
    18361 aaatcgtttt tatttgtaat gatacggctg tttttgaaag aaagaaaatg tctgctatct
    18421 tttgcatact gacataccct ttttgaaaca ataagtacgt cacaatagtt gaagctcgga
    18481 aatccatgct ggaatagatt ttatgtttcg tcacctcttg tatgatttgc tgtttagttt
    18541 cagattcaat tgattctaaa aaataacctg aacgtttatt tgactggata atgattttct
    18601 tctcgtagat agcaagcata tttttgattt cagtttggat cgttttcttg ttggtaccta
    18661 aagcttcagc taattcttcg cttgttatcc aatgattctt atttcttaat actacaagaa
    18721 tttggagttg tcgtttattt aagtccattt gcttacacct cctctttgtt ataaacatac
    18781 aggaaaaacc tctcaaatga caatagtcac ttgcagaggt ttaaaaatca tttgaagaaa
    18841 gatctatatt caaggttttt accattagac tggataattt gtttataata ttcaaaacta
    18901 tcttttttca atctcctaaa gcttccgctc ccatcatttt gtctatccac ataaataaag
    18961 ccatatcgct tcctcatttc ccctgttcct gccgatacga tatcgattgg tccccaccat
    19021 aagtaaccaa tgatttggca accatctttt attgcctcag caatttcctg aagatgtatt
    19081 tttagatact cacaacgttt tggatcgtgg attcgattca attcatctgg tgcttcatct
    19141 agtccaatgc cattttcaac tatgaataat ggtaattcat aacggtctgt caattcattc
    19201 atgacataac gaaaccctag aggatcaatt ggccaactcc aaggttcggg agatttttcc
    19261 ttcaaaaagg gattatcgaa accatttgct ccgccagtat ctacgcgcat ttctgcttct
    19321 tttttataga cagcgctacg ataataacta aatgcaatat aatctgccgt gttagcttgc
    19381 aagcactgtt cgtctccatc taacattact ggtgcacagt cttcttcctt ccatgtcctg
    19441 taaatataag ctggataatg ccctcggcac atgacatcgg agaaaaacca tgaattgcgt
    19501 ttaaattgca gtgaaccaaa tacattctct ggatcacagt catatgcata agtcgctaat
    19561 gcagaaaagc tacacataat acccatctta gcatttggat cgattttctt taataattga
    19621 gtagcacggg cgttcgctag aaacatatga tgacaggctt gataaatcac tttttttgtt
    19681 agtgtagcaa tgggttcaag tttatctcta gcagggaatg taaccaatcc agcagctgca
    19741 taaggcatac gaaaagcatt attgatctca ttaaaggtca tccaatattt cactttccct
    19801 tggtagcgtt caaagacggt ctgcgcataa aattcaaaaa agtcgattaa ttttcgattt
    19861 tgccaccccc catactctcc taccaatgct aagggggttt cataatgact aagagtaatt
    19921 actggctcga tatcatatcg atttaatgta ttgattagtc tgtcataaaa tacgagacca
    19981 gcctcatttg gcttctgttc gtctcctctt ggaaatattc gtgcccatga gatgcttgtt
    20041 cgaaaagctt ttaaacctaa ctccttcagt aaacgaatat cctcttcaaa tcgatgataa
    20101 aaatcaatcg cttcatgaga aagataatgg agtgattcgt ctaaagcaat ctcccaaatc
    20161 tttttttctt cattccactg aatactagga tgctggttca tgatcccgat aagcgtatct
    20221 gtcacattag gtaatttgcc gtcttccaac catgcacctt cacattgatt agcagctact
    20281 gctccgcccc ataaaaaatt ttctggaaat atttcctgat ccataactat ctctcctcgc
    20341 tatcaacaat ttcttacttg aagtatagct cgattcatca aaacaagata ttctaactat
    20401 ttcggcagaa gagaagaaaa aaattgaatg ctactcttca tttttaatat ttatatataa
    20461 caaatacata agacgatccc cgaaagaaaa cactcgttaa ccatctaagc aaacgattta
    20521 ttttacttcc aacacaaatg cttcataagg tcgtaaatca actttgccgt ctggtaactg
    20581 tttcagagga taatttgaca caataaccct taaagcttca cgtggaatag tgaacgtctg
    20641 cttttcactg gacatattac aaataacgag ccattgctgg tcttcatata tgcgagaata
    20701 ggcaaagaca tgctcaggcg tcttttccac taattcatag tctccccaga cgatgatgtc
    20761 atgttttcta cgcaaatcga tcaatttctt gtacatataa aaaacagaat cttgatctgc
    20821 taacgcttct tctgcattga tttctttata atttggattg actcgaagcc atggaattcc
    20881 agtagtaaac ccagcatgtt cacgggcgtt ccattgcata ggcgtcctag cattgtctct
    20941 tcctttcgcc ttgattgatt ccaagatttc ctcttttgta aatccagaag aaatcctttc
    21001 gttatacata ttgattgtct caatatcttt tgcttccgcg atatcatcga ttgggcaatt
    21061 cgtcatgcct aactcttctc cttgatagat ataaggggtg cctttcatca tatggagcaa
    21121 gatagcaaac atcttggcac tttcttttcg atacacatcg tcatttcccc aacgagaaac
    21181 aattcgcgga agatcgtgat tattccaaaa taaactattc catccttcat cacctaaaga
    21241 tgtttgccat ttggataata cttttttcag ttcagagata ctcaagggtt tcaaatccca
    21301 tttgtctttg ccttcttgct gatctaatcc tatgtgttcg aattggaaga ccatggataa
    21361 ttcatttctt ttaggattgg aataaagttt agcgatttcg gtagttgctc cccatgtttc
    21421 gccaacagtc atcagatttt tgtcaccaaa agttgcttga ttcatttcct gaagatactc
    21481 atgcagttta ggtccattac ttgtgatttc tttatccggt tctttgccaa tcatatcgat
    21541 cacgtccata cgaaagcccc caataccttt atcgatccag aaattcatca tttgataaat
    21601 ctcttggcga acctgtttat ttttccaatt caaatcaggt tgacgcttgc tatacaagtg
    21661 cagaaaatat tgccccgttt tttcatccaa ctgccaagca gaaccactga atattgagcg
    21721 caatctatta ggctcttttc cattgaccgg atcacgccaa atataatagt cccgataagg
    21781 attttcttta tttttacggg cctcaatgaa ccacggatgt tcatcagaag tatgattcac
    21841 caccagatcc atgatgatac ggatatttct tttctttgct tcttcaatca gttcttccat
    21901 ttcttccatc gtaccgaatt ctgccatgat tgcttgataa ttactgatat catacccatt
    21961 gtcatcattc ggagactcgt atactggaga aagccaaatt gcatcaatac ctaaatacgc
    22021 cagataatcc aacctggaaa taatcccccg caaatctcca ataccatctt gatttgaatc
    22081 ttgaaaactt cttggataga tttgataaac aactgaatgg tgccaccatt tttgttccat
    22141 gattttcctc ctataaatgg atgtttctat tttttgctta atcatttatt ttagtctctc
    22201 tcactaaaaa ttgtaacgct ttcaaatagc tattgcacct tgttatcggt aacagaataa
    22261 aacaaaaaaa cagctgagga aattccctca actgtttttt gaaaggattg atttatgcag
    22321 ggaagaatgc tgatttcaca acttctttat aaacggtgtt cactcagctg tcccttggta
    22381 ttagctcaaa aaacggcaaa atataagaaa ctattttttc gtttttttct ctaatactca
    22441 ggccagaacg ctgatttcac aacctcttta tttcacattc aatatccaca tgaatcccac
    22501 aaatacaaag aacagaccat acatgattgg agatacttct tttcctcttt tagctgctaa
    22561 catggtaatt ggatagaaga taaaacctaa tgcaatccca tctgaaatac tataagtcaa
    22621 aggcatgccg atcaagatca agaatgaagg gatcgcaatc tctaagtttc cccattcgat
    22681 ttgtcgcaaa gattgtgcca tcaatactcc taccataatc aatgctggtg cagttacttg
    22741 tgaagtaaca actgatagta atggagaaaa gaacaagcct aacaagaaga aaatccctgt
    22801 agtaatagct gtgatacctg aacgtcctcc aacggcgata ccagctgatg attctacaaa
    22861 tgcaccaaca ggagaagtac caaataagga acccgcaagc atagctgttg aatcagaagc
    22921 taaagctttc cctacacgag gcatcttgtt gtctttcatg aaacctgctt ggtttgctaa
    22981 tccaactaaa gttcctgctg tatcaaagaa agtcacaagc aagaatgtca gcactacgac
    23041 ccacatttga agagaattga tgtcccctac atgttttaat gcaactaaaa atgttggttt
    23101 caaacttggt gctgcggcaa caatttcaga aggcattgga atcaaacctg tgaccagacc
    23161 taaaatcgtt gtagctgcca tcccgatgaa gatgccacct ggtacacgcc gaacaagcaa
    23221 gatagcagtt acgactaatc caaaaatcgt caaccaagta gaaccaacgt tgagtgatcc
    23281 taatcctact aatgtagatt cattcgaaac gatgatccca ccttcgctca gaccaaggaa
    23341 agcgataaat agaccgatac cgccagaaat cgcatatttc aaatctgctg ggatcgcatc
    23401 aatgatcatt tcccgcagct tgaagattgt gatcaaaata aaaatcaaag aagcaacgaa
    23461 tacgccggct aatgctgttt cccatggaat ccccattcct aaacaaacag aataagcaaa
    23521 aaaagcattg atcccaagag caggtgccgt tccaattgga taacgggcta atacccccat
    23581 taaaacacag ccgatcgcac tggctaatgc agtcgcagta aacactgctc cttcatccat
    23641 tccagaagca cctaatacag ttggatttac aaataagata taagccattg agataaatgt
    23701 tgtaaatcct gccagtacct cacgttttac cgtggtgttc aatttttcta tttcgaagta
    23761 ggaaagtatt ttttccttca tcttccatcc ccctaaatcc gaacattata aataaagtcc
    23821 gttaaaattt cgatttatta ataaaacaac gtgttttatc atatactaaa acaaaacata
    23881 agtaaacaaa aaattcggat tttaagcaaa atccgaacgt tccatctgat taaatccctt
    23941 tatctcaaag ttcatgaaca ttcactttgg attacaagaa tttctttatt gtgttaataa
    24001 agtttcttca ttctagtctc aaaaatcaat agtgtgattt gattttacca tttcgtttct
    24061 gttcttcttt gatgaattct tttgtatcga caggattcag attatttagt ccgccaaaac
    24121 ctgtatcgac catttttccc cagccttttg tcttcttttt tcctgatttg agattctttg
    24181 ctcgaagctt ctcactatgt tttgctcgcc gatacttcaa ataaccttct aatcttccca
    24241 ttagatccac tgtttttcct cctttttatt caatcagacg actcagcttt tcctataaca
    24301 aaataattgc cttcagggtc agaaaaattg aaagtcaaga tgccattgtt atctgtgatt
    24361 tctcctggtt cttccatgtt ctcgtagata gtttcaaatt catccgtaaa aaatactaaa
    24421 gaaggcggtg gtcctaatac ctctggtgaa aacttctgca caaatacttt tgggaatacg
    24481 cctaattcta attcttttga gatgctgagt acaatactct taaattcttc tggcaattcg
    24541 attgtttcag aaatttctgc accaattttt tctacaaaga atcggcaaat catctctacg
    24601 tcattgacat aaagcatcac acgtgttttt tgtatcatca tttgaccctt ctttctattt
    24661 gattttcttt cctgtctata aactgattcc attatatcac gaaagcgcaa acaccttgtg
    24721 tttgatacta ttcaagaaaa ataaagcaat agtttctagg attgctttcc tccacaaact
    24781 ttttttacaa aaaaaaaacg gcaatgaatg ccgctcttct tatttcgatt cttttataat
    24841 tctgcttctg attcatcttt ggttagtgtg tgtctaacac tagttacgcc aaatgtttga
    24901 tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac gtgccgcttg ttcagcattg
    24961 atggattcaa tgaccagcac cgctgcttgt gtttcttccg tcagcatcgt tctttgggct
    25021 tcacgccagt tgagacagcg acagatcgct ccttgttcat catagtagat gatttcttcc
    25081 ggaagtgccg gagcatcgga atctgcacct aaaggcagga atgattcgcc accttgggct
    25141 tttcctaaat gcagatttcc tgcgatcttt tcgatatctt ctccgccgca aggtacgcca
    25201 aaagacaagg aaatactatt atagatatct acaagcggat tgattgggaa aaattcccgt
    25261 tcttgatttg cacgtttcag taaagcttca atagaagaac gtgcaccttt tttagtcttg
    25321 aatgtggtaa atgcctgacg ccactgagca atgacttcgt tttggctgaa tgtttcttct
    25381 gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata attgatgata cgtttcttca
    25441 ctttcttttg ttacgtggtt atcgatacca tttacggata agatttcgat ggttgcttca
    25501 ggaaatactt cccagaaatt ggtatccact attacttttt tcatctgttc acacctcttg
    25561 attagaataa ttgcttgatg aatagatagt ctgtttgggg atcatctccc atataaaaaa
    25621 catgttcgct gtatttgata aatccttttt tacgataaaa agcgagcgca acatcgttgt
    25681 gttcccaaac accgagccaa atggctgttt tttgcttttc ttttgctttt tcaatagctg
    25741 tatctagtaa aaatgtaccg ataccttgat gtttaaattt ttttcgtaca tagattcgct
    25801 cgatttctaa aaaatgatca gccatttcct cagattgact cgttcctgta tttagtttca
    25861 ggtaacccgc aggctcttga tcgatactca ctaggaaaaa atcggtttct ttatctttta
    25921 gctcttctgt aagtttttct agtgcataag cttgtgtcaa gtaatcagct aaatgctccg
    25981 gtgaattatc ttttccaaat gtatctgtaa atgtttcgat actaatttgt tgtagtaagt
    26041 ctacatctga aggcgtcatt tttcgaattt cgtatttatt cacggcttct tcctttcaat
    26101 agatcctttt attgcctttt ttgacaaaat tccaatcttc gctaacattc ttgcatactt
    26161 tatttaaaag atattccaat tgttttgctt cttcgtcaga aagaccattt aatgcgactg
    26221 cattggagta gttgttttct cgaatgatga aaggatagat ttctgctcct tttttagtcg
    26281 gatagagttt cttgattttt ttattttctt tatcttccaa acgttcgatc atcccattgg
    26341 attctaattt cttgattgct cgggcagtcg ttgtacgatc tactttgatc atttctgcta
    26401 gtttttcttg gatgattcca ggattttcgc aaatacgtac taaataaaga tactgtcctc
    26461 ttgttaaatc gacttctttg aattcgatat tgctaataga atctagcgct cgggcaatca
    26521 ttccgatgtc tcgaaggatt tcagccattt catcccacct ttattttgtt tgtaagacta
    26581 gttcacctct ttttgttgca aatgcaacta ttttcaataa aaaaatcaag ttctttttta
    26641 ataactagta aagtatattc ttttcttctt ttacgaaaaa gctcaatcac aatagatctg
    26701 gtttttgttt tctaaaataa gtaactgttt cttcgttgtt cgcttttctg atagctttgg
    26761 caactcttct atactaaaaa atcccatgtt ggaagtttca ttattctcaa taaacttggc
    26821 ctcttcaccg tggatcgcac aagcaaaaac aagtttataa tactgaaaaa gctgtggaat
    26881 atctttttgt ttattcgtat caaaaactgc tcgcaactga gtagtttcta ctgttaatcc
    26941 tgtttcttca tagacttctt tacgcacatt ttcttcagga gacaatccta tttcagcaaa
    27001 tccaccagga agtgaccatt ctttcgtccg tttatcttcg attagtagga ccttgccatc
    27061 ttttttgata taggcacgaa catctacttt tggtgttgga tgtccttctc caatcgttgt
    27121 gagcgctttt atttcttcga aagtttcttt tcctatttca ctgatcagtt ccaatgaaat
    27181 cgtccggatc tcctcaaaac gctctttatc aaatacatct tttccataat atagaccagc
    27241 ttgagaaagt gcgagtagtc ttttgtattg agccaacttc ttgttcattc tcttcccttc
    27301 ttttttagca aatgtatgac tatgtaatca agtatagaag actggtttaa ttacggcaaa
    27361 taaatataat gaaatctgca ataaataaaa gaatgttaaa aaactcgaga acaaaagaca
    27421 ttcgacttct ctggggctgt gaataaatgg cgttcaaaag cttgcatctg cggtaataag
    27481 ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg ctgttcttat taattgaggc
    27541 aggacagctt tttcacaacc tccccactaa acggtgttct cctaccaact cctcggtcac
    27601 aagtcacaat attcatcaaa catgagaagc tgtttttttg aaattgctcc ttgtgactcg
    27661 gagctgaacg acaggctcac gacctctcgg taaacagcgt tcaaaagcca atttttcgat
    27721 attaagccat ttctttgtta cttattgtca ttgatttctt tttctaattt atccaatagt
    27781 ggagtgtaat ccacttgagt atagtctgtt acatcaaatt catatagtgt tcctaactgg
    27841 aatttgtcat attttcggtc ggcgatgatc tttttgaatg cttctttcgt aatatgattg
    27901 gtccctttcg ttcgatcttc caatacgtca ccatagtgat aatgatccat gatatagctc
    27961 aaatgacgat ctgattcacg atcacgtttg taccgacgtt gccataacac ttcaaagtct
    28021 gctgttaggc gaatcgtgat cacctgatat ccgtatctat ctgctaattc ttggagtcgt
    28081 gctttttgct tatcactaaa aggatactcg gacacaatca ctttctttcc tgcttccata
    28141 tacaaatcaa gtgctttgta atagaaagga taaactttcg tcctttcttg ttccgcccgt
    28201 tcttccaaat tgttaaaccc gatggattct gcatagtatt ccttgatttc atcgagcgtg
    28261 atggcataca tatctggaaa tcgttcccgt agtttattca taagatatgt ttttcctgta
    28321 ccaggactac cagccagaag aatgaaatac tttttcatct catttatttc ctaagttaat
    28381 tggactttcc aagttcataa acagtccaat cttatgcttg acaatttctt ttactgcttc
    28441 atttgctgga atcacattat ggcgaagaga cttgttgatt tctgtttcac taaaggtttc
    28501 tttagctact ttcgtccagt tgactaaaag ctccgttcca acattgaatt tgcggatccc
    28561 gttattgatc aattcaggat aatcttcttc tttcacacct gttcctccat ggataactag
    28621 ggggacatca actactgcat cgatttcttt cagcaaggga atgtttactt ctgtttttga
    28681 tttgaattgt ccatgattcg taccgatagc aatagctaat gcatccacgc ctgtagcttc
    28741 tacaaatttc actgcgtctt ccggtcttgt atataccttg tcattttcag cgacatggat
    28801 tccttcttct gtaccgccga ttgttccaag ttctccttct acggaaacac cgtgcgcatg
    28861 ggcatattct actactgctt ttgtcttcag gatattttct ttgaaaggca gatgggaccc
    28921 atcatacatg acagaagtat atccgctgtc gattgcttct ttgatatcat caaagtctcg
    28981 ggcgtggtct aagtgcaaga ctgcatcaac catttcttct tctgcaatag ctgtcattac
    29041 attgactaac acatgcatgc cgatatattt agcggtatcg acacttgttt gaataatgat
    29101 cggcgaaccc atttcttttg ctgcgcctag catagcagga agcatttcta ggttatgcgt
    29161 attaaaagct ccaactgtaa aattcaactc ttctgctgtt tttgttactt ctttcaatgt
    29221 tgtatacatc ttttattgcc accttatctt tttatttgtt tcctttgatc gttacttgtt
    29281 ttgcaatttg tcgcatctca tccattttgc ccatgtagta atcgatccct tgatcatcac
    29341 cagcacgagc agcatctgca cgcttctggt tatagaggct catcagtttt ttatacccat
    29401 ctccgatcgt ctgtttattt tccaaactct tttctaaata ttggatggct tgatcgttca
    29461 catacaattc agaaaattta agtccaactt gttcataaag tttcgctaat ttatgcttat
    29521 cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg
    29581 cttctggcga tagggctttt tcttcttttg ccagaacttt tttcggttct ttttcttttt
    29641 tcttaaaaaa tccaaacatc attttcccta ctttcaatta aaatgctttc aaaactgggc
    29701 ttaagatcca ggcatataag agtgctgcgg ctactgtatc tacatcggtt gctgtagcat
    29761 tgttgaatcc catcgaatta aagatcgtta ctagcaaagc gggcaataat gtaataaaga
    29821 aaccatgagc aataccgcca atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata
    29881 tccctgctgt ccctccggca aagaaattgg ttagcatacc aggtaaaatc atcgctaagc
    29941 cgaacatggg caaagtaaac atggcaataa tcgtaccaat cgttgtcgta atgaaaccta
    30001 aaatgacagc atttgggcta taaggaaaga aaactggaca atctaaggca gggatcgcat
    30061 caggaactaa tttcatagct atccctcgaa atgccggcac gatttcacct aacagtagac
    30121 gtacaccagc tagcaataca tacacaccca ctacgaattg gatggcttgt aaaaaggcaa
    30181 acatgatata gttttgatct cctgataatt cagaagcgaa tggttcgcct gcaaataaaa
    30241 cagtgatgat atatagcggg accatcacga ccatgacaga caaataagta tcttgcagaa
    30301 attcgaaatg tgtcggcaac ttgatatctt cgatcgactt tttattttcg cctttctcac
    30361 caaaaagctt agcaacaccg gcttcaaata agtacccgat cgtacaaaaa tgacctaaag
    30421 caatatcatt tgatccagtg attttacgaa tgatcggttg cgctactgcc ggcatagcaa
    30481 tggcgaaaca agctccgata aaaccaccga ccaagatcaa tacgatccct cgcagaccgg
    30541 caaaatagcc aaagactgtc gtcatcgttg ccatccataa aattgcttga ccagttaaaa
    30601 aaatgtactt ccattttgtg aaacgagcca aaataatatt gaatacaaaa atggctaaga
    30661 acgtcagtgc aatatctcta cctaaaccca aatcattcat tgcttgacca ttgatagatt
    30721 caatagaagg gatgatccct tgcatatgaa atccttcagt aaagattttc ccgaaataag
    30781 ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac ttgaaatcct aggagtgtct
    30841 ttaacgtgcc agagatgact tgtcctgctg atttcttctg cagtagtaga cccaacatag
    30901 caatcaatgc aatggtgatc gatgcctgag tcaaaatatt ttcgatgata aaattgataa
    30961 cgcccatgtt tttaccctct tttctttttg tatttagata actcccttat cttgcagaac
    31021 tggggtgatt ttttcttcaa tttcattttt tgaaacaata tttttcaaat agatcatcgc
    31081 cgttttagga tcaactgcga acttttcgaa ctgcgaacga aaattttctg ctgtgatgat
    31141 cacatctggt ttcatggaaa ctgcggaaga aatatcacaa tggtccagtt tggcttctac
    31201 tcctaatttg ttcaaaacat cctcagcaga catctgtgct gcaaaactac ttcctaaacc
    31261 tgcaccgcat acgaataaaa tgttcaattt gttcatcttt gttctccccc gtatcattta
    31321 ttatttctca actactgaaa aaagcagttg tttgaaatct tcgatatttg tgcattcaat
    31381 caatcgatcc aacttctctt cgttgttgat caattcaatt agttctttca tgatgttcag
    31441 atgcgagtag gagtctactg ctgccaaaca aaaaataatc tttactggat ccatttcagg
    31501 attaccgaaa tcgattggtt gctcaatcgt agctacgctg actcctaatt tattgacccc
    31561 atcttctggt cgggcatggg ctagtgccat gtgtttgcca atcacgatat acggtccgta
    31621 atcattcact gcacgaatca tcgcttcgac atagcgctct tcgacaatgt tttctttaac
    31681 caatggtcta gcagctagtt caatcgattc ctcccaagta gtggcttttt ctttgatcat
    31741 gatattgctg tccgtcaaaa tatcttttaa cataggctgg atctcccttt tgttgatttc
    31801 taaattgttt gtttcgaata atttttctaa cttgccatag atgattcgat ctactcggcc
    31861 accactttct ttcatgacat ctattatttg agtgaataac tccgtcgatt catgactgtt
    31921 aggctctaac cgctgtagtt cttcatttct tttcaaaaag tcttctataa ccaagatatt
    31981 cgtctcagtt aaaatcggat cgacaactaa aagcggctta tttcgataat tcaattgaaa
    32041 ggtgctgaaa accagattga catccaattt atctactaat tcgatttctt ttgaacttaa
    32101 aactgcagta atctcgatct gaggaaactt ttctttcaga ttctctgcta acagatttcc
    32161 agtagccatc ccatggttac agacaacgac tgatttataa acataattca agtctcgctt
    32221 gattacactg gcgatcgtcg aaaagtggat gacaagaaaa gctatctcgt cctccagtat
    32281 cttgccacca attacttctt cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg
    32341 gaaataattt gttcggatgt tttctttcaa tggattcatg acttggatat gattcttgat
    32401 tcttacaatc agtgcagcca tgtgcttata gagattttcg aataaagctt cttcctttag
    32461 atggaaaggt acgtgtgtct cgttctctac aaattggatc aattggatcg ttagtaattg
    32521 agcttgtacc cactcgattg aattggagat atctctagca ttgaacgtct caatagtgaa
    32581 acgaagataa tttctttcta cttgcgggac attgtccaat tgaaattcct cgatcatgtg
    32641 gtcaataaat gcagaaaagc tactgtcttt tatttctgta tttttccaat taatggcaga
    32701 gataaattca tgtttcctga ttcgctggat ccagatcaat gtaaaaagca ggatttgttt
    32761 tttataaata tcatcttctt gtttcaacaa cttattggaa tagatctcct gtattttttt
    32821 gatttccacc aaaggaaaat aatggagaaa gattttttga gccggtgtta attgtgcatc
    32881 ttgattcata aagaagtcgg tcattcccag attctgattg ataaaatcat agatcatcgt
    32941 acggatagat cgttctgaac ctttataaac cagtccttgc tttccataac tgacgatctc
    33001 tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata tcttcatcta atgtactttt
    33061 agagatttga aattcttctt cttttttgtt tagatggatc gggattcggc tgtaagcaac
    33121 ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt
    33181 tatttcagtt tctaatcgag cttgcttttc cttttccagc ttcaacaaaa aacctttgtt
    33241 gcgaattgta cggatctccg gaagcttttt actgctcaac cagatattcg cttcaagaac
    33301 ttcctgacgg atcgtccgct gactgacatt gaactcactt gctagatgtt ttgcttttac
    33361 tggatattgg gctgtcatga ttctgtgaag tatttcaatt gttcttgttt tcatagaaat
    33421 gatctccttc tgatttcaag tatatggttc cgcttacata ataaaaagta gatatttctg
    33481 ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa ataagccata tgctttttca
    33541 tctcctcaac gaaaaagaca tacgcctatt attacaaaaa ttttgcccat ctatacaaaa
    33601 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acaactaaac taaaatagtg
    33661 cttcctattt ttcgtttggt tgttcttatt atcgcagatg caagctttta aacaccattt
    33721 agtggagagg ttgtgacaga gatgttcagc cttgagcgat aagaagcgat tttgaaaaat
    33781 cgcttcccat attttacaaa atcgtgcctt attgccgata ggctatctct ggcacaccgt
    33841 ttattcagaa aaaggagctg cgacaaggct tttgtcacaa ctccgttcat aaatatttcc
    33901 tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc atcttttttc tttctcagaa
    33961 aaatcaaaat accatatctg agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat
    34021 cgcccatctt ttcgatcgac tgaataatca cagcatctga aatcaattcg gatggcaaaa
    34081 ctattttcgt ggatctgtcg ataatttcct acttcctgaa tagttatttt tttagtatga
    34141 tttcttacac cgtctgcttt gtttttttgc agaactttct gatgttcggc aaacagcttg
    34201 tctgtataac agcttctggc tttttcagga gtttcttctt cccaagcgga ttggatcttc
    34261 atgaatatct tttggatttc cagaaactgt gctttctttt ctttagccgt tcctgacatg
    34321 cgggacaata tctccgcttt cagccttttc ttttgtcttt tattcttgac gatagaaaaa
    34381 gcagaaaccc ctcctactga caaaacagct aatgcaagaa atcccgtttg ctcataacta
    34441 gaaggacctg tacctggacc ataataccca taagaatctc tgccgtaaga acgactgcca
    34501 aaataactgc ccccatatga acgaccacca aatgaacctc tgcccgaact tcgaccgcta
    34561 gatgtacctc caccatgacc acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa
    34621 cataggccca ctaacaaaat gattgctaaa taattgattt ttttcattgt cttcctcgtt
    34681 atcttttatt acaaaaaact atttttatga tagccttgct aaaaggaaga gtcaataacc
    34741 agatgcataa actgtttttt tagcaatttt gtcttattta gtcattgagt aacatcggac
    34801 aaatgtatgt gtcggcagtc cataattcct ctatcttcag catgaacaag cttgcagaca
    34861 agagatctgc gcttcctcca ggagagattt ggttttcctg acaaaagtgc aacagtgctg
    34921 taaatggacg atttggtaaa gcactttgac tagcagcatg agcaagtttt tgcatttcca
    34981 ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc atctagcgaa ctcatcaaaa
    35041 ctaacaacat atgcatttgt ttctgtacat atgaatcatt atctagctcc ttagctgctt
    35101 gaattgcaag cctgatatgc ggatacccta atatcgcttc ttcatatgca ccatttacgc
    35161 catatttttg cttcacccta tgaccatgag tggttttttt atgacgatat tttgtatcag
    35221 gaaaagcagc aagcttgctg acatcatcaa gtattttcga taaagaaaaa ggctgttcca
    35281 tactgatttt ttgtgcaatg acactgatca atagccccat tgcccaaatc gctcctttat
    35341 gggtattgat gccatttgta gcaatcagca ttgccttttc cgcttttcgc ccaatctcag
    35401 caattctttc tcttaactcc tgatcaatag catgatgcca tgctatttga gccatttctt
    35461 caaaaaaagg agttagcgtc ttagcagact taagaaataa agcatagtcc atatcggaat
    35521 gtgcacccgc atccagtgca tccactaatc caggttttgg acttaatgtt acttctgcga
    35581 tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac tgcttttttt cgttcgtcat
    35641 ttatcatgac actcactcct tcttcctact attacttgct gaaacctttt ttgatagcta
    35701 cccaaatgat atgaagtaac cagcttaaag caaatgcaca acttgccgtc attagataag
    35761 ctacaagaat catgactcct gaaggtaact gcagccaact atctttaaac aataataaca
    35821 gtacttggaa aacaaataca ttggctaaat acgtacgata agcataaaca gacaaccatt
    35881 tgaacaatgg caattttggc gaatcgttta caatcatttt ggaagcgcct atgaagacag
    35941 caaagatgat gactaaactg tataaacttt ggatcgtatt caagtaaggt gcattcgcaa
    36001 agctcaagtc tcctggatgt gcgagcaatg aacggacaga aaagaacgcg ataactaagg
    36061 caacaggtaa gaaaagataa cgtactctgt ccaaatattg atagactttt tcgtgataaa
    36121 caaatgcggc taccccataa atcatgaaac tagcaacaaa ccgatctaat aagtaccagc
    36181 tgtcatgata aggcccagtc aatacttgtg tttgatagaa cagataccat gcgacatata
    36241 aaatcgttga accaatcaat actggccata catatttttt attagcaaat actttgtaac
    36301 cgagccaaac aaaaaacggc atgaacaatt ggatctgcag catcataacg gtataccata
    36361 aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa gcttgcagta tccgtatacg
    36421 gagtttgttg ttggactgct ggaaatagcc aaagataagc cgtcgtccat agaagatacg
    36481 gaacgactag ttcaaaaaac ttttctttca aaaattccca ataagacaaa tgctgacttg
    36541 tttttaccat attataaaca attgcaaaaa taaacattgg tgctgtgtat ttaacgatga
    36601 cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg gctgtttgcc aaagcaaagc
    36661 tcaatatcgt ttgcatcata acagccgtta cagctgatac tttctggaag tcacttacgt
    36721 tgattttgat ttttttgttt tcaaacatct ctttctctct ctttttcttt attaccaaag
    36781 ttctttggtt aatttaggtc cttcgactgt tttcaataga atttttttct ctggctctct
    36841 caaatactct tttagtgaaa aaccgccttg ccgagtcact acttgggtat cgaattgctc
    36901 aaaaaaagca ggatgcgatt gaattacctg aattggcaat tcaatcggtg aatttgcgta
    36961 cagcagcaaa tcaaaatcac tggtttcttt tactgccgga atcccagtcg ataattcaaa
    37021 tcctaagctg ccgccgattc cccatttaca tcctgataat atgtttctag cagcatgaaa
    37081 ttgctgataa acaggataat ccatgatttt ctcttgtcta tacgattctt gttcgatgat
    37141 gtcccacgga tgaatgactt gtttgacggc atgttttgag atttctgttg accatctctg
    37201 attgcgggca ggtccacgta aaccaatcgg tagtatttct gcgtgatccg tcgacgcacg
    37261 acgaactgtt ccgaacatct tttcagttag aacgacccat tcaggcaagg tgaacccttc
    37321 tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga atcatttttt atgcatccca
    37381 ttcttcgtcc attttggcac ggactttatt tgtttcgaca cgtccgaatg ttttggctgt
    37441 ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta ttgcggacat cttgaatggc
    37501 ttcattgatt gcttcgatga ctttgctgac attttgttct gaagcagctt ggtcggtgat
    37561 atcttctagg aaatgataga tcgctcctag ttttgtataa ttattgatat cgtaagcgat
    37621 agctggaact ttttcagcag ctgcttcgat ttcagcaacc gaacgttttg tcacacgtgc
    37681 agcactttct ttagacatcg cttggacggt aatttcttta ttgtttaaag caatcaagcg
    37741 gcttgattgt aatccatgtg ctaaaaatgc acctgaaata gcatttcctg caatcaatcc
    37801 aataactgga tgtccagctt gacggagttt tgcataagca gcagcactat tagccaaagc
    37861 cacatggata ccgatcaatt cttcgttata gccgtatgct tggcttggta catcgatcac
    37921 aacgatgatt ggccgtttca cttttttgtc tttatcttct tcgtaaacgt gatccaaaat
    37981 ttgtcccatg cggaaacctt caactaaacc aacttcaccg tttcgtacac ggtacatcgg
    38041 gtttttagga tcaggtacga ttgatgtcac gatggcttct tctatgaatt tacttgattt
    38101 accataatac acagtactaa tagaagaagt tggatttttc atgccagtta gtgccgcaaa
    38161 ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct tctgctactt ctggtaagtc
    38221 aattgatttt gcttgatggt tagcaattgc ttcattgtaa tcgtcaatgg ccattggttt
    38281 tgaaagatct aaagaatcaa gtaatgataa ataaaatgct gagctttcac tgcgatgcgt
    38341 gtcttttcgt tcgtccattg ctgcgatgac agcagctttg attgcttcta cactatcagt
    38401 cactaattca tcaatgattt ttgattctac ccgttgacga gatcctaatg tattccaaat
    38461 caagtctttg tcactagaat caaattctcg aacgcctgct tcttgttcaa taacttctgg
    38521 tccattcaaa ccgactctcg ctttttttgt tgcgattaaa taactcatta atgctgaagt
    38581 aatcgacata ccgccaaatg atcctacacg tcctggaatc aaaccgatga ctggtacata
    38641 ttgttttaga gcaatgatta catttccgat ttctgaaata gataacagtc cgtagtttgc
    38701 ttcttgcaaa cgaacaccac ctgtatctag tacaatgact ggaaagatct catttccttt
    38761 tttattttct tctaacacat gttcaagtgc agcaacgatt tttgcaccgg aaacttcacc
    38821 gataccgcca ccttggaatt ttccttccat agcgattact actgcttgtt tgccattgat
    38881 cgtgccacgg ctgacaacga tcccgtcatc actttcaggg acgattcctt gtgctggtaa
    38941 acttggcgcg atgatttgat cgaatggatc aatcaattca cgtccattgt caagtagagc
    39001 gttcactcgt tctctggcgt ttaattctac aaaactattt ttcatctgaa agttcctcca
    39061 ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc accgaagtca ttgatcacga
    39121 tatctgctga aattggataa cgatcaaaaa atctcgttaa tacattgttc catacttcgc
    39181 cgaagccatc gcttcctgta caaattttta cagtagattc tgttttttct gttggaaaca
    39241 tcaagatttc caaatctcct gaaccgacca ctcctacatg gattttctta ttgatcggct
    39301 gtttcgttgg atatttataa gctaattgtt ccatggatct ctcccctttt tgtaaaacag
    39361 agcatacacg ttctgcttat tcgtctttct tcttccatct gggtaaatgg cgacaaaagg
    39421 tttttctcgc caattttatg accaagttct gaatttcatt ggtggttcat acaggccatc
    39481 tgaccattcg atcaaatcat cgatggtttg tgcagctaat aatgaacgtt tcgcttcact
    39541 acgtttgatg cctagatctt ctggtaattt cacgatgccg gctgcacgca agctatctag
    39601 tgattctttc gtgctagtca ttccaagagg tgtaacaccg ccaattgctg caattgctgc
    39661 ttgtcgttct tcttttgttt ttgctttgta caaataagca attccttctt ctgtcacgat
    39721 atgtgtagta tcttcgctat aaatcatcac tggtgcattg gctaagcctg cttgtttttg
    39781 aacagctagt gcatctaatt cttcgacaaa gactggtttt ttatttgaac catatgtttc
    39841 aaccatttga acaactaatt tttggccttt ccctaatgga tcatcattgt cgcgaagtga
    39901 ttgccatgct ggtgtagaat gacgacgacc gccaggatta tgtcccatgt taggcgcacc
    39961 gccaaaacca gataaacgtc cttttgttac ggtagaagag tttccttcgt aatctaattg
    40021 caaggttgaa ccgataaaca tatcgatggc atattgtcct gcagcttgtg ataatgttcg
    40081 gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca tctgggcgag cagcaatata
    40141 tttttccata ccaacttcgc caccaaaact atggatgctt tcaacccaac ctgattcgat
    40201 ggctggaatc atcgttggat gtgggttcaa tacccagttc ttcgcgattt tcccttttaa
    40261 ccctagtgat tcaccataag ttggcagtaa caattcgata gctgctgtat tgaatccaat
    40321 accgtgattc aaagattgta cgccgtgttc tgcgtagatt cctttgattg ccatcattgc
    40381 cattagaatt tgcaattctg tgatgttttg aggatctcgt gtgaataaag cttctaattg
    40441 ataaggttta tctgctttca cgacaacgtc tacccagtca ccagggatat ctacacgtgg
    40501 taaagaatcg acaacttcgt taacttgaac gatcacgata ccatctttga aagctgcagc
    40561 ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg tacaagttgc cgtctttatc
    40621 tgctttatct gcagctacta aaacaacatt tggaatcaaa tcgatgaata agcgtccata
    40681 aagttccaaa taagtatgga tatcacctaa tttcattgtg ccgtcttcaa tcatttgggc
    40741 gacacgcaag ctttgtgcac cagcataaga gaaatcgatc ttgcttgcga ttcctttttc
    40801 aaaaatattc aaatgttctg gtcgtgagat actagacatg atcaaatgca gatcattcac
    40861 tttttctgca tctacttttt caagactttc tgaaagaaaa cttgcttgtt tttgattgtc
    40921 gccttctaat acaactttgt cgccgggagc aatcagtttt tccattactt ctacgatttt
    40981 ttcagtttct acatacttgc cattgagcaa gttgctgatt ttttccaatc gctgcgcttt
    41041 tttttcgcgt ttagaagccc agttgcggtt tggtttttcc ataattagtc atctccattt
    41101 ttgatataga aacgtggcgt catcaatacc atgttggtct agattgatcg tacggatccc
    41161 tttatctaca ccaaattttg tatgaatcag ctttgtcaaa gtattgccgg ggggaaattc
    41221 aatcgtcaga tccattccta attcttttgc caagtccatc atttggttcc actgcacagg
    41281 gaaagctagg ttttccaaca aatctttacg aatctcttct gcgtcctgag tggctcttcc
    41341 atccgtattt tttatataca aacatctagc atcagataga tggacagact gcatatacgg
    41401 cttgaatttc ttgaccgtat cattcatcaa gagacagtga gatggcacag ggactttcag
    41461 cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc aacgtaacgg ataaaccttc
    41521 gatactccca gataacgtat gttgcatcgg acaattttca tttgacagat aaactggatg
    41581 gtcaggatca aatgtttttg ctacaatttc ctcagcttcg cttctcgtca cacctactac
    41641 tacgcccatt gcataaccgc ttggataagc ttctttcatt aagactgcgc ggtgatagac
    41701 tagttccgct gcattctcaa atctcaaaga tcgacaagca acagctgcac aaaaagcacc
    41761 aatcgaatgt cctgctgcaa tatcaggctg aataccgttt ttttccattt catctaaata
    41821 aaatactgct tttgtcagta atgctagctg gataaaaacc gtatcttgat agttttcttc
    41881 tttatcaact aaccgatacc ccgtcacatt ttctaaacgt tccatgtaca tttcagggat
    41941 atccgctaac atccctaact tctgactgcc ttgtccagga aataagaaac ctgtactcat
    42001 gagcctcgcc tcctcagcac ttactttttc cactgctatc ttagtcaatt tctgctataa
    42061 taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt atggatggga tggataatta
    42121 tgaaattgct tcatctgcag tattttaaaa aagtattcga aacaaaaaat gttacccaag
    42181 cagccaaaga attatttatt tctcaacctg ccttgagccg tgcgatcaaa catttggaaa
    42241 acgaattagg tgtccctttg ttttaccata gcggcagaaa cattgagacg accgtttatg
    42301 cagaagaatt ttatccatat gcagtcaaga cattagacac attagaagaa ggaatcaaat
    42361 tattgtcttc ggtcaatgag aagatcgtta cttccgtcat attgtattta gaggtagcct
    42421 ctgtttctat tccgaacttg gtccgaattt ttatggaaaa gcatcctgat atccaattaa
    42481 cgatcatgca acatcattta ccagatgata ttaccgatcc tgtactgtac atcacttcgg
    42541 agatgaagcc tggcatgacc aatattccta tcatgaaaga acctgtttat gtcgccattc
    42601 ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt agaagatatc caacgcaccc
    42661 ccatcttgat gctgtcaaag agcaacgcct tccgtcatac actggatgca gcacttgaag
    42721 cgaaaagtat cgaattgacg atcagttcga cgacagatga tcctgcgaca ttacgttcta
    42781 ttgtcaagca aggattagct gttagctttt tcccaaaagt ttcttggtct tacgataaaa
    42841 atgatccttt cgtcttgcgg gaaatcaaag atttgccctt gacacgaaca atttatttat
    42901 cttctagctt cacagaagat catcctttga tcaaaatgat tgcaaaaacg atgcgggaat
    42961 tttatttagg aaaataaaaa aaaaagagct gggacaaaat tcttgtcgca gctctttttt
    43021 cgaacaaacg gtgttccaga agtaatttct tagaaaataa gccgaaattt cccaaaaatt
    43081 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctaaacattt ctgtcacaac
    43141 ctcttctcta ttgtcctaga cattcattgc gttgatttaa aacttccatc aatcaaatct
    43201 gctagttcat ctagcttttc tattttacgt agatcaatag agatccaatg ttctttattc
    43261 atgtgatagg ctggaaaata cccctctttt cctcgcaaaa ttccgccaag ttcaggatcg
    43321 accttcaaat caagaatatt gatgatccta tggtcacttg catcatcata gattttttct
    43381 tctggaacag acatcaggac cccaaaccat tttttgcttt tttgatgtct gaatacgcaa
    43441 taatcaggaa attttttaaa aatatattct ggagacaaat tgtatgtttc attgatgtga
    43501 tcgattagtt cttttttcgt tagcatctgc ttccatctct ttcttattga ttcgctttat
    43561 tcaaagatac cacgtaagaa tagaaaaatg aatacaagta gttgctaatc aaatacttcg
    43621 atatttttct tttttatcaa ctcttaaatg ttcaatccgc ctgtgttggt cttgctacca
    43681 tctactatta aaatgtttat tattgtttgc ccacctattt tttgatttta gtggtatcat
    43741 ttttcaagtt catgatgaaa ttcttatcat cagtaaatgc tccagaaaaa taaacaatga
    43801 ttgaaaaatc agttgaaact tgaccaccgt ttattttctc cgtattctta gaatagaagg
    43861 gagtctgatt gatgtattca ctaatgaaaa aaatcattac cgaaaaagat attcgacgtc
    43921 atgtttcttt agtagaacaa ttgctgaatc atacaaagat aaccgtaaat gaattagctg
    43981 aaatcatagg gacaaccgaa cgaacgattt tttctgacct tcaatctatt cgctctcagc
    44041 tgcccgaagg ctgggacatc atcagcgatc aagcaggaat cagtttgcaa aaccagcaaa
    44101 atttattaac aaatgacctt tgggagattt ttttcaaaca atcagtcagt gtcgaactgc
    44161 tgaaaaatct gctttttaca aaaaaagttg ctgtccctga ttttctagcc gaccatgggc
    44221 tttcttatgg gacattgaaa agacatgtga ctaaaatcaa tcagcgttta gcaagttacg
    44281 acttgcagat cgatttgacg aaatatacag cttgtattct tggaaaagag cgtgcgattc
    44341 gtacttttta tcatcgattg ctgattcctt tcactcataa caactatttt ttcgaggatt
    44401 attcgatcca tgaatcccat tattttcaat ttttaagaaa cctcagtcaa actgaactag
    44461 cggttgaaac agaagagatt ttcggaacat gttggttctt tatcaatacg atccgtataa
    44521 aagcgaattg ccgattagat tcttctatcc ataccaacag cacactctcc tccctgtatg
    44581 actctgcatt gaaaaaattg tatttaacgg aaggtattta tcttaaagac actgagcttt
    44641 cttttgcttc tttctgcttt ctggaaagtt ggaattacaa caataactat ggacaagaaa
    44701 ccgctcgctg cctgcatcat tctccatttt tagaggtcct cgaaacgttt gtagaggaac
    44761 tagcttcaga gctgtctctt gatcaactaa aaaaaacatc tctgactgat aacttggctc
    44821 tcctcatatt aaaatatcat gaatcgccga tattgattga agcattggac cgtcaatacc
    44881 atcgtttttt agaaacatat gatcagcaat atgcacactt gtatcaacag aaagagaatt
    44941 tactgaacga tctaaaaaaa gaaatagaaa tcaatgaacc aaattacttt ctccaactct
    45001 tgtctctact gatccaacaa acgatttttt ctatcaaacc gaatttgttc aatgtctatt
    45061 ttattttcca aggagaacct tcatggaagg cgttcttgca gcaggaatta aaagattact
    45121 taggaaaacg tgtctgcttc ttacctatcg aactaaccga tctttccact atttcttttg
    45181 aaaaaacaga tatcttgatt tccaatttcc ctctagattc tctagatatc cctattgtat
    45241 acatttcttc tattccaacg aaaaatgagc tcaatcgttt gactgaactc actttcaaaa
    45301 cttttttata gtaacggcta gcgactgtat tctctagctg ttttttcttt tttgcgaaac
    45361 agtttgttca tgatcccttt ttctatttct acaactaaga atagaagaat cacatttgct
    45421 ccaatcaata actgttgtgt catcgttaaa cttactgtac cgatcagctg ctggccgact
    45481 ggtagaaaaa taaccatccc ttgcaataaa gcaaggatac ctaatgaaag aaaaagcgct
    45541 ttgttttgca aaagaccttg gttcaatgac ggcttggaca actctctgca attgatcatg
    45601 taaacagctt gagctaagac gatattttgc agaagaatgg tttgctgtaa gctttgccct
    45661 tcaaactgca tagccaataa ataggcagga accatgatca gtagggagac atagatgatc
    45721 cgaaacaaat gataaccaga taggattcct tgctttggat ctcttggtgg gcgcttcatg
    45781 atttctggat ctggcgactc gaaccctagt gcataagata aagtgattgt cgttaccata
    45841 ttgacccata aaatctgtac tggggacaaa ggtagcgggc gattcagcat aagtgcccaa
    45901 atcaagatca atccttgagc taatgcagtt ggcaagaaga aattgatggt tttttgcaaa
    45961 ttatccaaaa tccgccggcc ctcttttacg gcttttgcaa tcgtatggaa attatcatct
    46021 cctaaaacca tatcagctgc ctgtttgctt acttcactac ctttgattcc catagcaatt
    46081 cctacatcgg atcttttcaa agcaggtgca tcatttacgc cgtctccggt cattccgacg
    46141 atttctccat ttttctgcaa agctttaaca attcgcagtt tatgttctgg tgtcgttcga
    46201 gcaaagacat cgactattgg cacttgctga atcaactctt catcagacat aagatccaaa
    46261 tcgatccctt ctaaaacttt ggcagtatgt tttaagccaa tttgttccgc aatcgctttt
    46321 gctgtgtctt tgtgatcccc tgtaatcatt ttcactgaga tccctgcttg ttggcattcc
    46381 tttacagctt tgactgcact ttcttttggc ggatcgatca atccggcgat ccctgcaaaa
    46441 gttaattggg ttaaagtctc atgcgtgagt tcctgtttcg aatctacttt tttataagca
    46501 aatcctaaaa cacgctgacc ttttctcgca aattctgcag ctgtctgact ccatgcttct
    46561 tgttctgcag aagaaagagt tgatttagca aataatactt ctggtgcacc tttgacgtaa
    46621 atcaccgctc ctttttcctc cgcatgcatc gttgccatat atttgtattc agaactgaac
    46681 ggaatctttg cttctatttt tttcaaaggt agattggctg tttctgtgtg tcgaatcagc
    46741 gcaacttctg tcggattgcc acttaaattt tctgttttct gttcttcttt tgtttttaat
    46801 tcctgacagt ttttcataat ggacaacacg caggcttctt cttttgcagc tacatccaca
    46861 actgtcattt cattttttgt caatgttcct gtcttatctg aacagatcac tgtcatagaa
    46921 cctaatgttt ctaccgaagg aagtcctttg atgatcgcct tttcttttgc catttcatgt
    46981 actccaacgg atagaatcat ggtcaaaaca gctggcaatc cttcgggtac cattgacacc
    47041 accaaagcaa tcgtagccga aagaagaata tgccaatcca taccatatcg aaatgtcgta
    47101 aaaataacta agaataggct caaacaaaga atgccttgaa aaatttgttt gtttaatcgg
    47161 ttgatttttt taatcagagg cgtcgtttgt tgttcgaccg attgcaatgc ttgattgatt
    47221 ttaccgattt ctgtcgtgtt tcctgtttct acaacgacac cgactgcaga accagattgt
    47281 acaagcgttc ctgaaaaacc catatttttc tggtcgccga tcgattcttc ttctgtcaaa
    47341 acggcatgac ttttttcaga tgcttctgat tctcccgtca aaatcgattc ttccactaat
    47401 aaatcatgtg cttcgattaa tcggatatct gctggaacga catctccggc acgtaagaca
    47461 atgacatctc cttttaccag ttctttcgca gggatttttt cttgcatccc gtttgatagt
    47521 actatcgctt cttgtcccat cagctgcttc aaaccattca atgattcttc cgcttttctt
    47581 tcttgccagt aactaacgag tccattgacg atcactacga ataaaataat acttccttca
    47641 atatattcac tagaagcaaa tttcaaaaaa gaggcaatga tcaacacgat catcagcaga
    47701 tcagtaaaat gctttgctat ttttttccat cgttttgtct gctgccttgc ttttatttca
    47761 ttatatcctt gatcagtcag gcgtttctgt cgttcttctt gcgacaagcc ttctgttgat
    47821 gtttgatagt ttttcaatac atcttctacc gtcaatctgt accagttcat cctatcccct
    47881 cctgtttaat tgatactgtt attatacgca gggaagtttt ttcaggcgta tcgattattt
    47941 caggggggtc tgaaataatt cgttttgcaa aatgtatctg ggcctaactg atatttctat
    48001 tgcttattct tttatataca aaatttaaga atacaaacca gataaccaat aaaaaaggaa
    48061 taaagcgtgt cgtgctttcg aataacaagc tgaaagcaat tgaagtaaaa aacaccaaca
    48121 aagcaagatc tttatatctt tgtgtagtgt tccgtttctt ttctttaata aattgaacat
    48181 gtgcacacaa gatcgaagac cacacaaata gaaaacaact cgttgtcatg cctgccaaaa
    48241 gctgaaaaaa caatcgacta tcagctacaa aaaaatgtag acaaagaccg gcaaaaaata
    48301 acattcctgt aattaaaata ccgtgtaaag gtacgccttt cttagaaagc gcacaaaacc
    48361 aatggggcaa acttttttct tttgctgttt gatataaaat cctgctagta ctgtagactg
    48421 cactatttgc tgaggacaaa gaagcaacca aaatcattag attgacaatt gctgctccgc
    48481 cttggcaatg gatcacttgc agcatagtca ctaaaggact ttcttttaaa tctaaacaat
    48541 gccatggaac aacaaataaa atcgacaaaa tcgttcctat atagaaaaag agaatacgcc
    48601 aaggcagaca gttgattgct tttttcaaat tcttttgcgg atcacttgtc tcgccaacgg
    48661 ttaagccgat catttcaatt ccagcaaaac tgaataaaac cataggaaat gagactacca
    48721 ttccagcaaa gccaaacggt agtaattcat cgatctgtcc aaaattttct ttgattccga
    48781 tagactgggt atgaaaatct gagacactca atcctatacc caataagata aaaaggaaaa
    48841 tccccaagat cttgatgatt gagaaaaaag attccaattc accaaaccac ttgactgcaa
    48901 caaggttgac tgccattaag atcattagtg cgctacctcc gcttatcaaa gctggtacat
    48961 gtacaaacca gaaatggata tacaatccta ttgctgttaa ttccgcaagt ccagctccaa
    49021 gccagcaaag ccaataagtc caattgacaa aaaatgccca ctttttgcct aaataatagt
    49081 caacgaaatc actgatagag ttaaatgtac tatttgctaa aaataaatca cccatggccc
    49141 gcatcaaacc ataaatagcc aaaccgcata tcaaataaac gagaataaca gctggccctg
    49201 ctgatgcaat tgcctttgct gaacctaaaa ataatcctgt tccaattgcg ccacctaatg
    49261 cgattaactg gatatttcga ttagttagag tccgtttcat tgccggtttt tccatcaagt
    49321 tcaccttttt ctttttcaag cagcatggcg acttcctgtt cccaagaagg tttctttatt
    49381 ttttcaatac agagtggtac tacaaacaaa atgacactga tggctgtgac tgcaattaga
    49441 ttgatccatg ttccagcttt catcgtcagt ccgacagaaa atccgattgc cgcaaataaa
    49501 acaataacga cagtccaaac tccaagattg ctctttttct ctttttttcc aattctaaat
    49561 ggtcgattca actttgcatg tgtataacgc aatctcaaaa ctgcgatccc catgatgaca
    49621 tagacgatac agtagataac agtagttgaa tttacgagca tcaaaaatgc ttgattgacg
    49681 ccaggaatca gcaaataaaa aagtgcaaag attgagataa tgacagactg agtcaaaata
    49741 acggatttgg atacgtcaaa ttgattcgtc ttccaaaaat tgaattttgg tggatacgca
    49801 ccttttctag cactttctgt cactgtcttt gctggtcctg aggcccaagc agataattgg
    49861 actgctacac caatgaagac aagtaaacta aacagattca caataagatg tggcaaacca
    49921 agaatttggc aataaatcga aatagattgc gcgatattat tcaattccat ttgtcctttt
    49981 ggtacgacat ttgctacaac taaagcattc aaaatattga cgaaaaatat aaaaattaac
    50041 gcggcaaata ctccgcgaat atacgtttta actggatttt ccaatcgagt gatatacaca
    50101 gaggacattt cgataccagt aaaaataaac atgatcggcg caaaataaac aaatgaggat
    50161 gcagtcgtag tatctggtat taatttgtcc catgaaaaac tgcctaatgt tccggttggt
    50221 acgatcccaa ctttgatcca cgcagcaaac cctaataaga gcatcatcgt caaaggcacg
    50281 tacaatccta accaaacacc gacttttccg ccgatttttg ccatatcaaa tttcaaattc
    50341 aaaaaagtga tgatccaata aacgactaaa atgacaacta atgtaaattt gctgttcaaa
    50401 ccaagcggca cattatcgat aatattacca aacaacggag cgagtgctga agcgaccatc
    50461 accatcccag gaaacatttg gacccataac agccatgaga caacgaaccc ccattttttt
    50521 cctagagcat tcgataccca aagttctgga ccaccttttt ggggaaacat tccagcaaat
    50581 tcaccagaga tcaatgagat tggaaaagcg tacaataata ctgcaaccag catgtaaaaa
    50641 atcatcgtcc agctcgttgc tgctacatct ggaatatttc gtacacttgc tacaagggcg
    50701 caggtcattc cgataaaggt catggtcgtg atttgctttg atgaaccagt atctttcatc
    50761 tttattcctt ctttcaatag cgacatatgt ttttgttttc ttttattttt gttcaagaac
    50821 agaaatgaca aggtttcatc gattcagtac aaaacccagt atcaccgaaa catccattga
    50881 acgatcttag tgaactgggt ttgatacatg aaacgaacga tccaacttat agaacgtttt
    50941 ataaaaaaga atatcttggc gaggaatata tgaaaaacag ggggaaatac aattgttttg
    51001 atgaagcaaa taattgtctg ttctccatat gtctcttgtt attctttcac acaaaaatca
    51061 gttctattgg atgatttcgt ttagtttctt ttccattgct ttggcgattt tttttgtata
    51121 aaaattgaaa attcgatcat cattcaaata aggagtcatg atagccgcac gtagcagagt
    51181 gattttctgt tcttttttcc attcttcttt cgttagaccc attctttcaa caaaacgaat
    51241 cggagaatca ccgtaatctt cttgtgtaaa tgtagtatga gaagtaataa accgttcacc
    51301 ataaacatcg ccatccatat aagaagaccg atcgaacatt ttttcattta attcattgat
    51361 agcgttcaaa tctgtacagc cttgttcttt gaatacccaa ttgaccatat taaaatcagg
    51421 atgattcaat ggatagactt cgattgtttt tcctttaaca gtaaaagtca gatgatcgag
    51481 aaattctctg aatctttgag ctgcctcgat tgaagcccca atcaattgac catatccggt
    51541 cacattcaat ggcaatacac gatgtgctgt ccagactgcg gcggcggttg ctccagcttt
    51601 tgatccttcc aaaatataag ctcctaacat atctggagcc tttactgatt tttcaaacac
    51661 atatggcgcg aaataagaaa tgatatttct catattttta tgtttgatta cgattcctcc
    51721 agcagcataa gggacatagc ccatcttatg aggatctatc gtgacagaat ccgcttctga
    51781 aatagctctg aacccttcat atacctcttt gtcaatcgtt acgcagtggt ggaaaacatg
    51841 atgttcttca aagaattcag ctaaggaagc atacggcaca aattctcctg cttcgtttag
    51901 aaacagtgaa cgcgcatagc cgccatatgc ggcatcaaca tgtaaataga aatagatccc
    51961 ttcatctttt aacctttctc ttaattggac gattttatct acactgtcta cttgaccttc
    52021 ttctgtcgtt cctacgacag caaccacacc tagaattggg attttttgtc cagctaattc
    52081 acgaattgtt ttttccaaag cattaatatc cattcgataa tcctcttgaa ctgggatagc
    52141 aaccatctga tctaagccga ctccgcaaat atctaacgcc ttcatccaag aatagtgttt
    52201 tgtttgaggg accagccatt tccctaatct ttggatattt tttccgctac gggaagaagc
    52261 tgctttgacc tcatccattt cttcgtccgt aaagctttcg gtcatttcca aaatttcttc
    52321 aacagataaa ttcaaaagtt cccactcaga catttttttc actttttcag gcagcacttc
    52381 cttgacagct aaaggaatcg atttgatgca acgggcgtac cataatcctt caagattagc
    52441 aatagaacca tctgctgcaa tgtgtcccca tccgtctgtc atattgaata atgaagcaaa
    52501 atcctgtcct acttctgctt ccatttgcga agttgccata gaagattcca atgcaacatt
    52561 atttgggttc cacagcatcg catagttgta agctaataaa gcaggcatca atgtttcagc
    52621 attcatttgt ccccagtaac ggcctgctga atgccatggg atcgatcctg cgcgtaatcg
    52681 ctgactgact tcatccaaga cctctatcat atggtcgcgg gtggctagat aatctggagt
    52741 gagcttatct ccttcgctaa ttgcaggcat atctgaagga atataatttt ctctccatcc
    52801 aagatgttca tctaccatct tgttcaacaa cattttataa actggtccat tttcagcttt
    52861 gtcgcctata aagacggcct tgatatccat atctttcatt tttcttccct ccatattttg
    52921 ttggtcaatg tcttgcagat acatgtgttt atctgtaaca cctcttcact atagcgccaa
    52981 aaatgagaat ttttttttta taacatcaaa tccacgtaga tttttacaaa caaaaataaa
    53041 taaagtcgga tttttctgat aattcaacgc aaaaaagcga acaatttttc aataaatcag
    53101 tcttctaaaa tagataacct aaaaaaagcg aacaaacaaa taaaatgact gtccttcatt
    53161 cactaaacat atttttattt ttttatattg ttaaaaattc gcaatttatt ttttaataga
    53221 attatctgtt ttccaaattt tataacataa aaaacctttt ttaacggcat ttatatgctt
    53281 tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg
    53341 caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt
    53401 aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct
    53461 ctccaatctc ctgcatgttt gcaaaaccac tttactcaat gatatcaata acataaaaaa
    53521 agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt
    53581 ttcagaacat atacctcgat gcaagatcat gcagcaattg tctcaaaatt cgttgttttt
    53641 aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg
    53701 tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag cttattttaa
    53761 tgactgcggg atcgaaatcg accgctactc ccctcgattc actgaaatgg aaagacgtct
    53821 gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc
    53881 gttttttgaa cgtgccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta
    53941 tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca
    54001 agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta
    54061 caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga
    54121 attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt
    54181 gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa
    54241 agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt
    54301 cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc
    54361 agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat
    54421 tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg
    54481 ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta
    54541 tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac
    54601 tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta
    54661 tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt
    54721 ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc
    54781 tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag
    54841 atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg
    54901 acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc
    54961 aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca
    55021 gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca
    55081 ttgttctcgc tgactcggtc gctttgcact tttgtatgga caaataaata tttcaaaaaa
    55141 tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc
    55201 agtacaagtt tttcgaaata tggaagaaga aactgcatca actctgtcgt catcctacct
    55261 ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg
    55321 ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg
    55381 actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca
    55441 gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc
    55501 aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa
    55561 tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg
    55621 ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc
    55681 gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc
    55741 catttttctc cctccatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa
    55801 taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc
    55861 caaatttcta aaaatagaac catacacctc ctcaaatctt ttcttcttac ctaccctatt
    55921 tttatttaca ggaaaatcag acacttcttc ttttttgtca aaatttcctt tttcttctcg
    55981 tcctatcatt tggcattcta ataaaaacga actatccttg gatatagaaa gagaaaacaa
    56041 ggaggcgttt ttcatgagta aaaaattaat caaagtcggc atcggtttag gtttgcttgc
    56101 ccttggcgct gcatatctag gaaaaaagac aggactattc gaagatgaca gtcatttata
    56161 tgatgaattc gaatcaatct agcatcctat ttagttgaat cactacggac atacttggaa
    56221 gacattggag gaatcacaat gagaaaaagt aaagcaataa tgattggtgc cggtattgcc
    56281 aatatggcag cggctgttta tttgattcaa gaaggcaaat ggaaagggga tcagatcacc
    56341 ttttattcat tggatgatca cggttcaaat gatggtgcac cgaccgacac tgtagtggat
    56401 gaatattgga ataaaaacca tccattagaa aaccaaaaag gctatattgc tcgaggcggt
    56461 cgcatgctta attaccgtac ctatgtagat ttgatggatc tgttaagtcg aataccttct
    56521 gttactgagc cagggatgac tgctgaagaa gatacgagag attttgatgc aaaacaccga
    56581 acctttgaca aagcacgttt gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg
    56641 ggattcaata ataaagaccg tattcttctg actaaactga ttgctatgcc tgattcagaa
    56701 gaagaaaaac tagacaatat cactatcgaa gaatatttca aagatgatcc acactttttt
    56761 gaaacaaact tctggtttat gtgggaaaca acatttgctt tcagaacacg tagttctgct
    56821 caagaattgc gtcgctatat gcatcaaatg atttatgaat tcactcaaat cgaacatcta
    56881 gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag
    56941 tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc
    57001 aaagaaacac cgatgcaaga tgaaatcact gtgactggtt tgaaaattac caatacactg
    57061 actcaagaag aagaatacgt tgtggtcgat gaagatacag cagttatttt cactaatggg
    57121 tccattacag attcagctac tttaggagat tacgaaacac cagctgtaga aaatatggat
    57181 tatggtgcag cttctagtct ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat
    57241 cctgacaaat tctttgctga tcgagatgca agcgaatggg tcagctttac cctgacgacg
    57301 aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc
    57361 ttgaattcat ttatttcaac cacaccgatc acgccgttag gccaaaaaga tgtcaatatg
    57421 tctatcgtcg tgcatcatca accgcatttt acgtctcaga aaccaaatga aacagttatt
    57481 tggggttatt tcctttatcc aagacgacgt ggcgaatttg tggataaaca gtatattaaa
    57541 atgaatggaa aagaaatgct agaagaactg attggacaac tttcaaaagt agatcctgga
    57601 ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca gcgtaataaa taatatccct
    57661 gtctatatgc cttatgcttc cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa
    57721 gtcattccta aacattcgac caatctggcc tttacaggag aatttgtcga gcagccttat
    57781 cagatgatct ttactgaaca aagtgctgta cgttctggag aaatcgctgc tttccatttt
    57841 gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt acgacaaaga tattcctact
    57901 ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag gttgtgaaaa agctgccctg
    57961 catcaagtaa taagaacaac caggctaaaa cagcttctta tgtttttgat ttggttggac
    58021 ttattaccgc agatgcaagc ttttgaacac cgtttaccaa gaggttgtga aaaagctgcc
    58081 ctgcatcaag taataagaac acccaagcta aaactgcttc ttatgttttt gacttggttg
    58141 gacttattac cgcagatgca agcttttgaa caccatttac caagaggtta tgaaaaagct
    58201 gccctgcatc aagtaataag agcagccaag ctaaaacagc ttcttatgtt tttgacttgg
    58261 ttagacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa
    58321 ggcgtttagt ttcgagtatg aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat
    58381 aaatttgact taataccgag aaactgatcc cttctgtcat aacctctttt tatttatgtc
    58441 tgtttctttt tgaaaacaac tattttttca acgtttactc tcttgccaat ccaacatcat
    58501 ctaacggata caactatttc aatgattacc attacactaa aaaatcggaa acacacctct
    58561 ccgaacacaa atacataaga ctatagaatg tttcatttaa gaaaaaaagc gtttcattat
    58621 caaaaaggta agatctcctt taaaatgaaa ttatctgtag tactaataat gcagacaatt
    58681 ttatgaatct gaaaggagca tctccaatga aagcagtcac ttggcaagga aaacagaaaa
    58741 tggaaatcag aaacgtagat gatccaaaaa tattagaacc aacagatgca atcatccgta
    58801 tcacagcaac agccatttgc ggctccgatc tgcatcttta ccatcatggt gaagcagttt
    58861 tggaaaaagg gtatgttgta ggacacgaac caatgggaat cgttgaagaa gtgggatctc
    58921 aagttcaaac attgaaaaaa gggcaacgtg tggttatccc ttttaatatt agttgtggtc
    58981 attgtcattt ctgcctgaat cacatggaaa gccaatgtga tgaatcaaat ccagatcagt
    59041 tatatggcgg gcttttcgga tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg
    59101 aatatctaag agttccttat gcagactcta cttcatttgt tgtaccagat aatgatcttc
    59161 ctgatgaaaa agttttattt ctatcagata ttttgcccac agcttattgg agcgttgaaa
    59221 acgcaggagt aaaaaaaggc gatacagttg tgattctagg ttctggtcca gtcggtctat
    59281 tcgctcaaaa attcgctgtt atggctggag cagagcgcgt aatcgcagta gatccagtac
    59341 agcaccgtct agataaagcg gctaattaca atggggttga aacttatttc ttagaagaca
    59401 cggcacaagc tggaacagaa ttgtatgaac tggctaaagg tggcgcagat gtcattatcg
    59461 attgtgtagg aatggacgga ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct
    59521 tacaaagcgg cacaatctct ccattacaga tggcatcgca agctgtcaaa aaatttggaa
    59581 cagtccaagt aactggtgtt tatatgactc cagcttcttc ttacccgctg caagaatttt
    59641 ttatgcggga tatcgctgtc aagcatggac aggcaccaat tatccatcta atgccaaaaa
    59701 tttacgcaat gattgcagaa ggactattcg atcctacaca aatcattact cattcgctgc
    59761 cactatcaga agcagcacaa gcatatgaga tctttgacaa aaaagaagat aaaaatataa
    59821 aagttgtttt gaaaccttga attcagtaaa aaatacaggt gattttcttt atgcccaaaa
    59881 tcaaacgaaa tacccatgag atcgaaatag gaaaagaaga agatatcgaa atacgaggac
    59941 gtcgcttccg cttctatttc caaaatcgtt acacattgat ctcactggct gtcgatttat
    60001 taaccgggat tttctatatt tttggaagcc ttgcttcctt aactaaaatc cccgatcgat
    60061 atggcaccta tttttatttg gctggtgcta ttttcttgac gattcgtcct attctcagaa
    60121 tcgtaaggaa catttttatt tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa
    60181 aagaggcatg agaatttttc aaatctcatg cctctttttt caccctatcg tatcactttg
    60241 atacagacga gcggtcgtcc cactttcttc cttgaccatt gtcaaaaatg tccactcgta
    60301 tcgttcataa aacgtggtat gatctgtaat taagtaaagt ttttcgtacc caagttgatg
    60361 catttctttt ctgatttccg ctaatatccg cttggctatc ccctgcttac gcttttccgg
    60421 ttcaacaaac agggcacaaa gatttggtgt cagatcaggg cggttatgga agtcgttttc
    60481 aatgacgccc gctcctccga cgatggtttc tttttcatct aagaccacat accattgagg
    60541 aatactttcc ttattagcga ttccagtacg aatactttcc tcatacacaa cagcaggaac
    60601 tccccatttt gatgaaaacc aattcgcgct ttgttttact aattctgggt gttctcttaa
    60661 tttgatccag ttatatgtaa ccaatgttta aacctccgac taaaataaaa cgtttacaaa
    60721 aattatacaa gtctttatga aaattagcaa tgacaacaat agtgatgtaa aaaaaaaacg
    60781 gaaaacaatc acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc
    60841 tttcttggat cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat
    60901 tgacagcggc tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg
    60961 gtggagttac ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa
    61021 agtaagcaac actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt
    61081 tcaacacgat aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa
    61141 tcggcaagtt tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta
    61201 acagtaaacc taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt
    61261 tgttcacgat aaacggtgcg ctatgcccag ctgcgattgc attaacgttt gcttcaatgg
    61321 ctggcgcatt gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga
    61381 accataagaa tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc
    61441 ccataaatgg ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg
    61501 cagcaaaaat tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg
    61561 aagcaaatgc ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt
    61621 catttccgct taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa
    61681 acaaaccgtt tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa
    61741 tcacacctgc ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga
    61801 aagcaatcaa gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt
    61861 tccatgattc accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt
    61921 tattgatcat tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt
    61981 cccgaatcgc aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt
    62041 tctccataaa tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac
    62101 gcgttcaatc gacgatacag atcaatgatc tcttttgcta aatctgtaaa agctatagat
    62161 gtcatcagat gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt
    62221 gcttcttgtg tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca
    62281 tctttgattt tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc
    62341 attgcatcgc tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt
    62401 ttttgatctt ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc
    62461 tgcttggtcg agtacttttt caccattcat catgccatag tcagccatat tgattacatc
    62521 taaaggaatg cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg
    62581 cggtcctaaa agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc
    62641 agatacagca aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt
    62701 gactaggaga cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat
    62761 tttaaacacg ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta
    62821 aaagcgcttt aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac
    62881 taaaaaagaa agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac
    62941 gtgcacaaaa aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc
    63001 aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta
    63061 ctttgctatt cttattactt gatgcaggac agctttttca caacctctca ataaacggtg
    63121 ttcaatcagc tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc
    63181 aaataaccag taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt
    63241 caggcaaatc caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca
    63301 atgttctgct tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg
    63361 cattcattgg tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc
    63421 cttcttctgt tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat
    63481 catcttgcac gattcctttc tcatccatat actgttcttc atattctctt cccataccgt
    63541 tttctgttat cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat
    63601 acatgatttt cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat
    63661 caaagacttc ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag
    63721 gggcgcgaac gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct
    63781 gaatcgtttc taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact
    63841 ccaggaactc ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa
    63901 acaaatcata catataagca gctttttggt cttctggaga attggatcga gcatagacca
    63961 tttcaggatt taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta
    64021 ctgctttagc cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt
    64081 gttcatgcgg ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt
    64141 tgaatgtaaa ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact
    64201 tagcataaaa atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag
    64261 ctgggacttc ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt
    64321 cgatcatttc acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag
    64381 taaaaaaacg agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca
    64441 gatccacatc ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat
    64501 tatgccaaac aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt
    64561 tttttccttt ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt
    64621 ttggaatagt aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga
    64681 tttgctactt ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc
    64741 gtaataactg caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt
    64801 ggccaaggga ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca
    64861 atcgttccag tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg
    64921 atcggcatac caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac
    64981 ttggtgattt gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg
    65041 atcgtgactc cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg
    65101 ttcggtactt cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc
    65161 aacggttccc gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact
    65221 tgcgcaataa acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg
    65281 cgttgtacga cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata
    65341 aatcccgcag ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta
    65401 gcagaaacat tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc
    65461 aaccagcaat aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc
    65521 gtatctgtat attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact
    65581 aattcataaa cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat
    65641 acaccatcca ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg
    65701 gtcaagaaac ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa
    65761 aaacgcaaga aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac
    65821 aatgtgccaa aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc
    65881 accatgatat accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat
    65941 ttatccataa aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc
    66001 tttttgtcta ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt
    66061 taccaaatat aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact
    66121 gtcaattagt ttctttttct cctattattt tctgtaattc tctccgattt attgccatat
    66181 ggacagtcat tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct
    66241 ctgtatgaat atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca
    66301 aagcaattga ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt
    66361 ctgcaatcat cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa
    66421 aagaagtttc aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat
    66481 attccgctgc caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc
    66541 gtatcatccg atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc
    66601 gcttatcact gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta
    66661 gagcccgcac agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat
    66721 attcattcgg cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct
    66781 tgtcagtagt tacttctact agggttttat tttcttccaa accacatacc tccttttcat
    66841 ctgatgtatt ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata
    66901 caaaattgat taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga
    66961 ctagga
//
LOCUS       DACJGB010000005        64972 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.005, whole genome shotgun sequence.
ACCESSION   DACJGB010000005 DACJGB010000000
VERSION     DACJGB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.005"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            299..1960
                     /locus_tag="IYI34_000357"
     CDS             299..1960
                     /locus_tag="IYI34_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0558259.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IYI34_000358"
     CDS             complement(2030..2377)
                     /locus_tag="IYI34_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAR0558260.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IYI34_000359"
     CDS             2461..3402
                     /locus_tag="IYI34_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558261.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IYI34_000360"
     CDS             3403..4446
                     /locus_tag="IYI34_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558262.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IYI34_000361"
     CDS             4452..5522
                     /locus_tag="IYI34_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558263.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IYI34_000362"
     CDS             5512..6465
                     /locus_tag="IYI34_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0558264.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IYI34_000363"
     CDS             6477..7487
                     /locus_tag="IYI34_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0558265.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IYI34_000364"
     CDS             complement(7772..8593)
                     /locus_tag="IYI34_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAR0558266.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IYI34_000365"
     CDS             complement(8690..8968)
                     /locus_tag="IYI34_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558267.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IYI34_000366"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IYI34_000366"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAR0558268.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IYI34_000367"
     CDS             complement(11724..12311)
                     /locus_tag="IYI34_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558269.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IYI34_000368"
     CDS             12486..13808
                     /locus_tag="IYI34_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0558270.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IYI34_000369"
     CDS             13963..14739
                     /locus_tag="IYI34_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAR0558271.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IYI34_000370"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IYI34_000370"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAR0558272.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IYI34_000371"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IYI34_000371"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAR0558273.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IYI34_000372"
     CDS             16629..17258
                     /locus_tag="IYI34_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558274.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IYI34_000373"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IYI34_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAR0558275.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IYI34_000374"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IYI34_000374"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAR0558276.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IYI34_000375"
     CDS             19445..19981
                     /locus_tag="IYI34_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAR0558277.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IYI34_000376"
     CDS             complement(20021..21784)
                     /locus_tag="IYI34_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558278.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IYI34_000377"
     CDS             complement(21787..23502)
                     /locus_tag="IYI34_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558279.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IYI34_000378"
     CDS             complement(24281..24505)
                     /locus_tag="IYI34_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558280.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IYI34_000379"
     CDS             24705..27473
                     /locus_tag="IYI34_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0558281.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IYI34_000380"
     CDS             27554..27973
                     /locus_tag="IYI34_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAR0558282.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IYI34_000381"
     CDS             27985..28455
                     /locus_tag="IYI34_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0558283.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IYI34_000382"
     CDS             28472..29251
                     /locus_tag="IYI34_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0558284.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IYI34_000383"
     CDS             29238..30059
                     /locus_tag="IYI34_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR0558285.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IYI34_000384"
     CDS             30076..31125
                     /locus_tag="IYI34_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR0558286.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IYI34_000385"
     CDS             31138..32142
                     /locus_tag="IYI34_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR0558287.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IYI34_000386"
     CDS             32775..33794
                     /locus_tag="IYI34_000386"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAR0558288.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            33837..34718
                     /gene="galU"
                     /locus_tag="IYI34_000387"
     CDS             33837..34718
                     /gene="galU"
                     /locus_tag="IYI34_000387"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAR0558289.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            35018..35395
                     /locus_tag="IYI34_000388"
     CDS             35018..35395
                     /locus_tag="IYI34_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558290.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            36001..36615
                     /locus_tag="IYI34_000389"
     CDS             36001..36615
                     /locus_tag="IYI34_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR0558291.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            37388..39127
                     /gene="yfcC"
                     /locus_tag="IYI34_000390"
     CDS             37388..39127
                     /gene="yfcC"
                     /locus_tag="IYI34_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAR0558292.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPEGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            39284..40468
                     /gene="iadA"
                     /locus_tag="IYI34_000391"
     CDS             39284..40468
                     /gene="iadA"
                     /locus_tag="IYI34_000391"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAR0558293.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            40579..40782
                     /locus_tag="IYI34_000392"
     CDS             40579..40782
                     /locus_tag="IYI34_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAR0558294.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(40883..41545)
                     /locus_tag="IYI34_000393"
     CDS             complement(40883..41545)
                     /locus_tag="IYI34_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558295.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(41558..43060)
                     /locus_tag="IYI34_000394"
     CDS             complement(41558..43060)
                     /locus_tag="IYI34_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558296.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            43608..45287
                     /locus_tag="IYI34_000395"
     CDS             43608..45287
                     /locus_tag="IYI34_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAR0558297.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            45414..45893
                     /locus_tag="IYI34_000396"
     CDS             45414..45893
                     /locus_tag="IYI34_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAR0558298.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            46112..46792
                     /locus_tag="IYI34_000397"
     CDS             46112..46792
                     /locus_tag="IYI34_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAR0558299.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            46800..48131
                     /locus_tag="IYI34_000398"
     CDS             46800..48131
                     /locus_tag="IYI34_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR0558300.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            48146..49108
                     /locus_tag="IYI34_000399"
     CDS             48146..49108
                     /locus_tag="IYI34_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAR0558301.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            49219..49557
                     /locus_tag="IYI34_000400"
     CDS             49219..49557
                     /locus_tag="IYI34_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAR0558302.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            49636..50343
                     /locus_tag="IYI34_000401"
     CDS             49636..50343
                     /locus_tag="IYI34_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAR0558303.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(50446..51927)
                     /locus_tag="IYI34_000402"
     CDS             complement(50446..51927)
                     /locus_tag="IYI34_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR0558304.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            52236..52772
                     /locus_tag="IYI34_000403"
     CDS             52236..52772
                     /locus_tag="IYI34_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0558305.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            52850..54220
                     /locus_tag="IYI34_000404"
     CDS             52850..54220
                     /locus_tag="IYI34_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAR0558306.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            54287..54622
                     /locus_tag="IYI34_000405"
     CDS             54287..54622
                     /locus_tag="IYI34_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558307.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(54703..55560)
                     /locus_tag="IYI34_000406"
     CDS             complement(54703..55560)
                     /locus_tag="IYI34_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAR0558308.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            55667..56182
                     /gene="tadA"
                     /locus_tag="IYI34_000407"
     CDS             55667..56182
                     /gene="tadA"
                     /locus_tag="IYI34_000407"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAR0558309.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            56205..56289
                     /gene="ffs"
                     /locus_tag="IYI34_000408"
     ncRNA           56205..56289
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IYI34_000408"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            56499..57884
                     /locus_tag="IYI34_000409"
     CDS             56499..57884
                     /locus_tag="IYI34_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAR0558310.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            57921..58598
                     /locus_tag="IYI34_000410"
     CDS             57921..58598
                     /locus_tag="IYI34_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAR0558311.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            58606..59370
                     /locus_tag="IYI34_000411"
     CDS             58606..59370
                     /locus_tag="IYI34_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAR0558312.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            59372..60031
                     /locus_tag="IYI34_000412"
     CDS             59372..60031
                     /locus_tag="IYI34_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAR0558313.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(60061..60570)
                     /locus_tag="IYI34_000413"
     CDS             complement(60061..60570)
                     /locus_tag="IYI34_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAR0558314.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(60682..61680)
                     /locus_tag="IYI34_000414"
     CDS             complement(60682..61680)
                     /locus_tag="IYI34_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR0558315.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            61881..62465
                     /locus_tag="IYI34_000415"
     CDS             61881..62465
                     /locus_tag="IYI34_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR0558316.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(62542..63717)
                     /locus_tag="IYI34_000416"
     CDS             complement(62542..63717)
                     /locus_tag="IYI34_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR0558317.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            63799..64179
                     /locus_tag="IYI34_000417"
     CDS             63799..64179
                     /locus_tag="IYI34_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAR0558318.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            64253..64615
                     /locus_tag="IYI34_000418"
     CDS             64253..64615
                     /locus_tag="IYI34_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558319.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT        21233 a        11189 c        13382 g        19168 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gccacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgtcgt ttaggaggaa cagcaagtga
    33841 aggtcaaaaa agcagtcatt cctgccgctg ggctaggaac acgttttttg ccggcaacaa
    33901 aagccatggc aaaagaaatg cttccaatcg tggataaacc aacgatccag tttattgtgg
    33961 aagaagcatt ggattcggga atcgaagaca tcttgatcgt aacgggaaaa gaaaaaagac
    34021 ccattgaaga tcactttgat gcaaatatcg aattggaaaa caatttgcgg gaaaaggaaa
    34081 aaacagaatt actcgcttta gttgaagaaa cgactcaagt caatcttcac ttcatccgtc
    34141 aatctcatcc aagaggactg ggggatgccg tgcttcaagc aaaagcattt ataggaaatg
    34201 aaccgtttgt cgtcatgctg ggggatgatt tgatgaaaga tgatatccca ttgactcaac
    34261 aattgatgat ggattatgaa gaaacacaag cttccgctgt tgccgtcatg cgtgtaccag
    34321 aaaatgaaac atctaaatac ggaattattg atcctaaaac agatagcgaa aagggtcgtt
    34381 gcagagtaaa agggtttgta gaaaaacctg aaatgggaaa agcaccaagt aatttagcca
    34441 ttatcggacg ttatctactg actcctaaaa tatttgagat attagaaact caagaaccag
    34501 gagcaggaaa cgagatacag ctaacggatg cacttcagac tcttaaccag acagaggcgg
    34561 tctatgcgag agagtttaaa ggaaaaagat acgacgtagg cgataagtta ggatatatga
    34621 agacaaatat tgaatatgga cttcaacatt cggaaattgg gacttcatta agtgcatata
    34681 ttatccagtt gagtaaaaag ctaagaacag aaagttaact ggaaataagt ggcctgctga
    34741 gaatgaaagg ctatttgtta ttaaagcggc agcttgtgcg tcatttgcgc aactgtcgtt
    34801 ttagtttttt gactctagat cagttgagtc gtggaaaacg aaagatagaa aaagactttc
    34861 gtgaatagat tcagaaaata ttatgctaat ggtttaattt ttttttaagt tgtataaaag
    34921 aaaatgatta cataagagaa atgaaagcca agccagaaga ttcatagtag aataaaaagg
    34981 cgaatttttt aattataaaa aaaggtgtgt gttcactatg ggaaaattag tcgctgttgc
    35041 acagtcgctg cttgatatac tcaagtggct gatggaagga agcaattgga tatttgtgtt
    35101 gccactattg cttattagtc tatttatcat gtttcgcatg gtcagaaaaa aagaaacgtt
    35161 tctgattttt atctggtatg cagcatccgt tggggttgct accttgatgg ttgaattggc
    35221 gttaggtagt tgggaagctt gcgtatttgc tttatttttt gctgtactgt atctaatcat
    35281 ttatatccca tatatgaaaa gaaagtctcg gataggcaga aaaggtagac atacaactac
    35341 tttttctagt acagattctc tcgaacaaag tagaagaacg agaagaagac ggtaatattt
    35401 agtaggtata cgcaaaaaaa gataaaataa tggataaact attgagatga gagatttatt
    35461 taattggtgg aaaaaagctt ttttcactta tataaaagaa tcttttttaa aggaatgctt
    35521 gttgtatcgg gataagccat tccttttttt atgttattta tatatatata aatcatacca
    35581 atgttttttt gtattttctt gcttgcactt ggataagaga ttacttacaa taaattcaga
    35641 tagaaacagc tgaataaaaa aaggctccct gacccttagt tggcgctaag aatcagggaa
    35701 tgaccagtca cacacataca ccctgaccga tcattttgcc atagtcaatg cagtgcatcg
    35761 acctaattta ttgtacctaa tctagggtga aaattctacc cctatgtaga tattttccat
    35821 ggtctgcacg ttgttttatt tgctatgtca aggtattggg cgtattcgtt caatgccttt
    35881 tttgtgtttg aaacagcaaa gaccacatgt gttactgtct aactactgtt tttttatgta
    35941 cttccttaga ttaggcagct gtttctattc atgtcaagta tagctaggcg ggagagataa
    36001 atgagtttat gttattttga ctattatcac caattggaag agaaattaag gatagaagag
    36061 ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa ttaatcaagg tggtagattg
    36121 cagatatatg ctagccgcac actaagtggt gtagcatttg aattttggga tcaaaccccg
    36181 gatcttgtac caagtatatt gattgaaaat ccagccggag gtgtctatga aacgctagaa
    36241 ggtgcaggac aggcaattat tgatcagctt tctgtcaaat cagaagacat ttttcttctt
    36301 ctttcgaatg aaggaagaaa cccggcaatc atcgaactag ctcagtggat caaagaaaat
    36361 ggtcatccat taatcattgt tactggattt gatttgtcac gaagtatcaa atcgcaacat
    36421 agcagtggtt tgcgattatt tgaatttgct gatttggttt tggacaacca cgccaattta
    36481 catgatgcag tcttgacttt gccaaatcta gatccagcga tctgcggaac agcctctctt
    36541 gcaacgatct ttttgctgca gcaagttttg tattttacag caaaacagct agtccaggac
    36601 acaagtggaa aatagttatc cacatgttga caaagttcct taaaaaaata atgttccaca
    36661 taaaaatgga gcgtttgaaa aaaagaaaac gattattaat gaggattaac aacgactact
    36721 ttttttgttt tagttatttt aagatggatc atctttgatt ttaagcttgt tttgtttaaa
    36781 gtttgttatt tttataagtt gtttcacagt tatgagaaat gagttataag aatatgtagt
    36841 gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag aatccatatg gattctttct
    36901 tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa ctctttacaa agtaattata
    36961 gtacatttgt tatcattatt gcaatgaagt ttacctgata taacaaaatt gtccaacagg
    37021 ataccttttt ctaatagaaa taaaatacac aaaaattgaa ggatagataa tggatattta
    37081 taagtgttta ttattttttt tgcaatgaaa ttcaattttt caatgattgt aaaatgaaaa
    37141 aaacaggacg agattttctc taatctaaaa ataaaaaaag aattttaagg aaaaacagag
    37201 aaataaaata cttaatataa atggaaagag aaaaaaataa tcatacatag gagaaaaaaa
    37261 acaaaataga aaaaagtgat tttctaataa attattcatg tggaaatgtc atttttactg
    37321 tataattcat ttgattatcc attttttatt catcaaaatt actgaaaaaa gaaaggaaga
    37381 aataaatttg aagaaaacaa attcttcagg aaaaccgaaa cgcgacttca ctaaattatc
    37441 gacaccccat acgtatgtca ttatttttgg tgtagtcatc tttgcatgga tactgacttt
    37501 tgtcgttcca gaaggaaagt tcagcacgca agatattgaa tacaaagatg caaatggcga
    37561 aacaagtact cgtactgttt tacgtcaaga ttccttccgt tatgcttacg agctggataa
    37621 gtcgtatgtg tttgatcaat tagaagaact gcaagatcat ccagcagaac gtgaaaaact
    37681 ggatgttccg gaaaaaggat tggaaaaagt cattgctgac ggagaaaaaa atcttacgca
    37741 ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa
    37801 tatctatgat acttctaaaa aattgcataa aacagccaaa atatggggaa cagatgattt
    37861 tggcggcttt ggtttcttaa attttgtttt tgaaggacta gtatctggag ataagtatgg
    37921 atctgctgta gggatcgctg cattgatcct tgttgttgga ggtgcttttg gtattattat
    37981 gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc agtaaaacta agggactgga
    38041 gcgtttagct ttaccgctat tattctttgc tttttcattc ggtggcgcta cattcgggat
    38101 ggcagaagag gtaattccat tctcaatggt gatggttcca tttgtcattg cgttaggata
    38161 tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct caagtcggaa atgcgacctc
    38221 ttggatgagc ccgtttagcg tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc
    38281 tggtgcgaca ttccgattga ttatgtgggt agttgttact gcattagccg ctggttatat
    38341 gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt tcgttgactt acaaatcaga
    38401 tgattatttc cgttctcata tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg
    38461 gcataaattg atcttattag agatgttagt tgtccttgtc tggatcgttt ggggcgtgac
    38521 tcaaaaaggc tattacattc cagaaatcgc ttctcaattc tttgtgatgg gattagttgc
    38581 tggaatcatt gctgttcttt tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc
    38641 tttccaatct ggtgcagctg atttagctgg tacagcaatc gtagtaggta tggccaaagg
    38701 gattcttctt gtcttgggag gatctgatgc atctgttcct tcagctttga atacgatttt
    38761 gcatgggatc gggacggcgt tgaccggtgt tccagctgtc attggtgctt gggcgatgta
    38821 catcttccaa agtttgttca acttggttgt tacatcaaat tcaggacaag cagctttgac
    38881 tatgccaatc atggctccac tggcagattt agtaggtgta tccagacaag tagcagttct
    38941 tgcttatcag cttggttcag gttttatgga cgcttttaca cccgtatcag ccagcttgat
    39001 tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat
    39061 gcaaggattc ctgtttgttt taggaactat tttcatcatg attgccattg caataggtct
    39121 gcagtagtac tggagacctg taaataaaaa agtaatccaa acagtagata aggactccaa
    39181 gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc tatcgtttgg attatttttt
    39241 aagatcctat catcagttat acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca
    39301 aattgaagtg tttgcaccag attacttagg aaaaatggat gtactgattg ccggtgggaa
    39361 gattttggct gtagaagaac agctagaagg cggctacgaa ggagtggaag tagaagaact
    39421 ttcaggtgag ggaaaagtac taaccccggg tttcattgac tgtcatttcc atattttagg
    39481 tggaggagga gaaggcggtt atcaaaatcg aacgccagaa gtcacattga gccaattgac
    39541 tacagctggt gtaacgacag ttgtcggttg tttgggaaca gacggagaag gtagagatat
    39601 gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa ggaatctcca cttatgtata
    39661 cgaaggttcg taccgattgc cggtaaaaac agtaacagat tctatcatca aagacttttt
    39721 gacaattgat aaaattatcg gaatcggaga aatcgcagta tccgatcacc gttcttctca
    39781 gccaagtttt gaagagttcg ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc
    39841 cggaaaagct gggatcatca atgtccactt aggcggagga aaaagaaagc tagagctatt
    39901 gacacgaatc gtagaagaaa cagaaattcc gatcacacaa tttttgccta ctcatgcaaa
    39961 tcgcacacca gaattatttg aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga
    40021 cttcactgct agcgaagatc ctgatttttg ggagaagaca gatggtgaag ttcgcttcag
    40081 caaagcactg aaacgattga tcgaggaaga tgtttcttta gataatttca cgatgacctc
    40141 agatggacaa ggaagcttgc cttattttga tgaaaacaat catttcctag ggttaggagt
    40201 aggaagtgcc aaggcattgc tagtagggat caaagaagct gtgcaaaaag aaagtatccc
    40261 tttggaaatc gccttacgag ctataaccag taatccagca cgtatattga aattggataa
    40321 aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt atcttggata aagaaacgct
    40381 tgatattgat acagttatcg ctaaaggaga aataatggtc caagaaaaag aagtcaaagt
    40441 atgggggact ttcgaaaaaa gtttttaatc aacagctcta actatctttt ttagaaaacg
    40501 agcgttctgt ttgtctgtat ctgttgttga tgttaccctg aaggtagatc aataaacaaa
    40561 ctaagaggag gaagcacaat ggcagattta aaaggacgtt ctaaagatat gaaagataaa
    40621 gtagtcggtg aaggaaaaga agcatacggc aaagcaacag acgacaagag caaagaaaca
    40681 gaagggaaag cacaatcact aggtgctgac atcaaagaaa aagctcgaaa tttaggcgac
    40741 gaagtcaaag acaagttttc tcgaaaagac gaaaaaaaat aaaaaccact tattactaat
    40801 tgaatcaagc aaataaaaaa tccgaaccat atctgcgaga cagatatggt tcggattttt
    40861 tatttgatca ttatcctgtt ttttaaactt cgattttttg aaatttttta ttttttagtt
    40921 caaagacttc atcacagctt tttgctactt caatctcatg agtcacgatg ataatacagc
    40981 gattttgttc atgggcgatt tcttggaaaa gtttgacgat atcttgtgaa gttgtttcat
    41041 ctagatttcc agtaggctca tccgctacga ttagttgatg ttcacagcag atagcacgaa
    41101 cgatagcgac tcgctgctgc tgacctcccg aaagtttggt cacctgtttt tttgctaata
    41161 tttcatcaat tcctacacga gccagtcgat ctaaagcaac ttgtcgataa ttctcttgct
    41221 tggtcttagt gatttccatt gctgctatga cgttgtctag agccgataaa taaggaagca
    41281 agttgtaagc ttggaaaata atcgagacat catttttacg ataacttctt aacccgattt
    41341 tgttcagtga acgatcttga tagtatacct ttccttcttt tggtgcatct agtccagaaa
    41401 taagcgaaag aaaagtcgtt tttccagaac cacttgctcc taaaatagcg tacatttttc
    41461 cagcttcgaa attcagttct acatctttat aaagcgcatc ttgcggatct tgataccaaa
    41521 atcccaattg ttctgtccgt aacatcaaat tcctccttta gtttaatagg atttttcttg
    41581 ggtttaatct taatatccct atagaagcta aaaagacaga aaacagactg attccaaatg
    41641 ctactccggc taatagtccc aattgtgcag gtttgacatt gatttccaat tcgctgactt
    41701 gatcagaaac agcaaatgga ttattcgtgg ttccttgttg accatcgcgt tgaccgcctt
    41761 gttcttgtct gttacctggc atttgtccac cgccaggagc ttgctggttt tgtgtcgtgg
    41821 tttctgtctg ttggtcaagt agctgctgtc ccacggcatt cgcgacgacg tttccgctaa
    41881 gcgaagcaac tcctaatgca agaatcatac aaatagccac ttcggtaaac aattggagaa
    41941 tgattttact tctggattct cctaaagata gcaagacgcc taactcatgc cgacgctccc
    42001 gaatggtaat cataatgatc agagtaagta tgataatacc ggctacagcc actagtaaaa
    42061 caacattttt agaaaaactt gccacgttgt ttaaaggttc caacattgac tgatacatgg
    42121 aatcatttga ttgtaagcta aatgtatctg tgtcgatcag cttctcagct tctttgagaa
    42181 actcatccat tttatctgga tcagatagag tgtaaatagc agaatctatc gtgttatcgt
    42241 cagatgtccc atttagttca tttgcaaaag tgtaagaagt gtaaagtgta ttcgacggat
    42301 tcatgaagtt gaatttcata cccatactgc cgcttgtttc gctggattca taaattcctt
    42361 tgatcgtcat ttcataagtg gtatcttcat cttcagtact cgtgattgtg aaagtatctc
    42421 cgactgccag gtcatttgtt tctgctagag tagaatcaat gactacattg tttgtgcctt
    42481 tgtcgctgtc atctattcct tcaccatccg tgatttttgc tgttccatca gaaaaattac
    42541 tattttgtgc agttgctgac acaccactga tttgaaagtc tgcttgagtc atttgcggat
    42601 tcccgcccat tcctccagca ggttcaccgc ctgcatcagt agtttctgaa ctgtcttcac
    42661 ttgaatcaga actagtgatg gcagttattc catcttttgc catagctgag gcggaacttt
    42721 caaaggaata actctttaca ttatctaatt cagctatttt ttccgcgtcc gttaaggaaa
    42781 ctggtgtaag agaaaaactg cctggatctg gacgttcttc agtggtagaa ccgcttgaag
    42841 aagtttgttc ctgtttttta aatgaagctt ctcgattggc acttaaagtc actgttgctc
    42901 caacttcttt ttgtgcttga ttcgctgctt cttctgcagc gcttcgaatc gtgaggccag
    42961 ctagcacaaa aataagaatg gctgtgaaca cggcaaacaa taacaatgaa cgtccccatt
    43021 ttacttttgt acttttccat gcacgtttca taaaattcat attttgcatc cctcctagta
    43081 gatgcatttg ataatagaca acctatctta aagctagctt aaatcttatg ctgtttgcac
    43141 accatcaaca tgaggttctg tatatataaa aagtcactat agttattgca aaaaatgtgg
    43201 taaaatgaaa aaagtatgtt tcaaaccgac gaagaagaaa tgaaagagga atagcaaaag
    43261 aaatcctaga tgctacagag ggatttttac gtgcatagtt gttctaacaa ttgctgatac
    43321 tagcgattta gtttatttgt tgtataataa tggggaacag ctgaatactt atataagtat
    43381 tctggctata gcgtttcgca aaagatccat tttgttaatg aagtatatcc aaaataaaat
    43441 gcccatccaa ctgtcagaac gcggatgtgc tggtaatttt cctgctgatt ctccataagt
    43501 tgagaaatag tgattttgca tatgcttcat tggaatggct catatattta gataaagaag
    43561 aaaatcgaca tctttaaact tggatgtcat aagaagaggt gaagtttgtg agtaccatta
    43621 aaattatccc attaggcggc gttcgtgaaa atggaaaaaa catgtatgtc gcagaagtgg
    43681 gcgaggatat ttttatttta gattgtgggc taaaatatcc tgaaaatgag ttgttgggga
    43741 tcgacgtcgt aatccctgat tttacgtatt tagaagaaaa cattgatcgt gtagctggtg
    43801 tctttttaac acatggacat gcagatgcga tcggggcact tccttacttg ttgtcaaaaa
    43861 tttctgtacc agtattcggg acagagttga cgatcgagtt agcaaaattg aatgtcagtc
    43921 gtaatgaagc agccaaagga tttaaggatt tccatgtggt agatgagcat acagaaatag
    43981 actttggtga aacagtagtg agctttttcc gtaccactca tacgatacca gattcagtgg
    44041 ggatcaatct gaaaacaaaa gaaggtagta tcgtttatac gggagatttt aaatttgacc
    44101 aaacggctat cccaatgtat caaacagatt atgcacgatt ggcggaaatc ggcaaagaag
    44161 gagtcttagc cttactaagt gaatcggcaa atgcagaaaa tcctcagcct gtagcttctg
    44221 aagcccaaat agcagatgaa gtatttgata cgatcaaata ttgggaaggg cgtatcatcg
    44281 tcgcttgtgt agcaagtaat ttgcaacgag tgcaacaagt attggatgca gcagaaaaag
    44341 cacaacgaaa agttgtcttg actggtcaag atttcgaacg aattattcgc acagcgatgc
    44401 gtttagaaaa acttcaatta ccaagtgaag atctgcttgt tacgttgaaa gacatgaaaa
    44461 actatgatcc agaagaactg atcattttag aaactggccg aatgggtgaa cccatcaagt
    44521 ctctgcaaaa aatggcaaac ggtacacacc gtaatgtcaa gattgaagaa ggagatttgg
    44581 tttatatcac tactacgcca agcatcgcga tggaaaccat cgtggcgaaa acggaagata
    44641 tcatttatcg agctggtgga acagtgaagt tgatctctga gaatatgcgc gtttctggcc
    44701 atgccaatcc taatgacttg caattaatga tcaatttgat gaaaccaaca tattttgtgc
    44761 cagttcaagg agagtatcgt gaattagctg ctcatgcaga tttggctcat gcagtaggga
    44821 tgccatacaa aaatatctat attactggac gcggagacgt gctggaatat aagaacaagc
    44881 gtatgagtgt tgcaggaact acaagtgcag aaaatgtaat gattgatgga ttaggcatag
    44941 gtgatatcgg aaacattgtt ttacgtgatc gaaaaatctt gtcagaagac gggattttcg
    45001 tagcggttgt cactatcagt cggcgagaaa agaaaatcat ttcaaaacct caaattactt
    45061 cacggggatt cgtttacgta aaagctagcc gtgatctgat gaaagaaagc agcaatatga
    45121 ttgaaacaat cgttgagaaa catttagaaa gcaatgattt tgagtggagc aaattaaagc
    45181 aagacatccg agatcaatta ggccgttact tatttgaaca aacaaaacgc cgcccagtga
    45241 tcttgccagt cattatggaa gctactcaac gtcgaaataa aaaataaaca aaaagagtaa
    45301 gatgaacctc tctatagagt gctcgtcttg ctcttttttt gcgaaaaaca aataattttt
    45361 gaatacagca tccctcgctt tataataaac gatagaataa agggagtgaa tcaatgatga
    45421 gtcaaacaaa agtcgaacgt tatttagaag aacaaaagat cagctatcaa ccacttgatt
    45481 ttagtcatgt ggtagaagaa tctttcacag aagaactaca aaaaagaggg attgatccag
    45541 ctttgatatg taaaactctc gtagtgaaag gaaacaaaac tggggtgatc atcgcattga
    45601 ttcctttgaa cgatcactta gattacaaaa aaacgagaca agtgacgaaa gatcgtaaag
    45661 ttggttttcc tggaatggat tttgtattaa tgcatacagg atatccccac ggagcaaaca
    45721 cccctattgg aataaaactc gcacatcctg attattgttt tttagcagac agttctttaa
    45781 aagaaaaaga agaagtaatt gtgtcaagcg gtgaaatcgg gagaagcctt cggataaaag
    45841 tagccgattt agaaaagatc gtccatccta tttacgctga tttaattagt taagggacga
    45901 gaagagaagc taagcagagc agagacctca aacttgagat tatatctgct tggtacttgg
    45961 tgcatcctgt attatctgtt acaattgctt tgtacatact gtactatcaa tccaatgaaa
    46021 atatatttag cagctatatt ttttcattga tgtgcaagtg atgaaatcgt ttttaataaa
    46081 aaagatacag agaaaacgcg aggtgtaaaa tatgaaaatt acgtatcatg gacattcctg
    46141 tttaacaatt gaaatggaaa atggccaacg cgtgatgatt gacccgttta ttacaggaaa
    46201 tccattgaca gacttaactg cagaagaagt aaagacagat tggttgctga ttactcatgg
    46261 tcatagtgac cacataggag atatgattcc tattgcaaaa gaaaatcaag ccacaattat
    46321 cagtattgtt gagattgctg attatgcgaa cagcagagga gtagattcat tcggtatgaa
    46381 tatcggtgga aaacatgcgt ttccattcgg cgtggtaaaa ttcgtacatg cacagcatag
    46441 ttcaagctat gaagtagacg gtatcgttca gtatatgggc gagcctagcg gaattattat
    46501 tcaagcagaa ggaaaaacga tttatcatgc aggggatact gcttatttca gtgatctagg
    46561 attgcttgca gaagaattcg atattgatgt cgctttcttg ccgattggcg ataactatac
    46621 gatggggcca gaagatgcag caaaggctgc gcaaacaatt cgtgccaaga aagtagttcc
    46681 aatccattac aatactttcc ctgtcattga acaagatccc gaatcttttg tagagttact
    46741 ggaagatagt gaaggaaaaa taatgagcgt ctcggaaacg ctggaagtgt aggcagaaaa
    46801 tggcgacaaa acatgatttg attttagaat atattgaaag tttacctgtt ggcaatcgaa
    46861 tttctgtaag aagcatcgca aaagaattga atgtcagtga aggaacagcg taccgtgcaa
    46921 tcaaagatgc agaaaatgtc ggactagttt caacaattca acgagtaggg accatccgta
    46981 tcgaacgcaa attaaaaaaa catattgaac gtttgacatt tggcgaggtc gtcaggatca
    47041 ttgaagggga cgttttaggc ggggcggcag gattagacaa ggtcttgaat aaattcgtca
    47101 tcggcgcgat gactaaacat gccatggaac gctatattac gccaggatca ctaatgatcg
    47161 tcggcaaccg ggcggaagtc caaaaattag cattggaaga tggcgccgca gtattgatca
    47221 caggaggatt caatacgact gctgagattg caaagctagc tgatgatctt gaaatgccga
    47281 tcttacgcac ctcttatgat acatttacag tcgcaaccat gatcaaccga gcgttaagcg
    47341 accaattgat caaaaaagat atcatgcttg tcagtgatat ctatatgcca gctgaaaaga
    47401 ctcattattt gcagcagaca gatacgattt ctgattatca gcgtttgtca gaagaaacgc
    47461 agcattcgcg ttttccagtc gtcaaccgcc atcatcgttt gatggggatt gttacggcaa
    47521 aagatgtttt agggaaatcg ccaaaccaga tcatcgatcg ggtgatgacg aaagaaccaa
    47581 tcagtgtaaa gaaaacaatg agtattgcct ctgttagcca tcaaatgatc tgggatgggc
    47641 tagaagtcat gccggtcgtt tccgatgact tgacattgga aggcttgatt acccggcaag
    47701 atgtaatgaa agcgatgcag cttgtccagc gacaaccgca gattgcagat acgatttcgg
    47761 atcagatatc tggaaatatc catacgattg atactgaccg tgaaggaaat cgtttggaaa
    47821 accctaagtt caaattcgta gtagcaccac aaatggtcaa tggcgttgga accatttctt
    47881 ttggcgtact tagcgaaatc atttctgatg tcgcacaaaa aacaatggtg atgaaccaaa
    47941 aacgaaatat cttgattgaa caagtgaacc tacattattt gcggctgatc caattggaaa
    48001 gcgaattaga cattcgtcca cgtgtattgg aaatcggcag acgttccgct aaactggata
    48061 tcgaagtgtt tattgaaaac accatcgttt ccaaagcaat cgttgtttgt caagtaatgg
    48121 aacgttctta gaaagcagga gtgaaatgac agtacagcaa gaaatagtag atacaataaa
    48181 aaaatacgat aaaatcatta tccacaggca tttgcgccct gatcctgatg cattaggatc
    48241 acaagtagga ttagccgaac tattacggga aagtttccca gaaaaagaaa tacgtcaagt
    48301 aggtgaaacg attgaagggc ttcgcttttt agcagaaatg caagaagttg aagatgtctt
    48361 ctaccaagga gcgctagtca tcgtgactga tacagctaat tcacccagaa tcagtgatca
    48421 aagatatcag ttggcagaaa aactgatcaa aatcgaccat catcccaatg atgaccctta
    48481 tggagatctt gtgtgggtaa atacaaaagc aagtagctgt agtgagatga ttgcagaatt
    48541 tgctttgatg tttcctgaat ctttaaagat gaacgttaag gctgcacggt tactttatgc
    48601 aggaatcgtc ggagatacag ggcgcttttt gtatccctcc acgacgtccc gtacattaga
    48661 gattgcttcg atattgagaa attatccatt tgatgcaagt gctttgaaca gagagattga
    48721 acaaatgccg atgaaagtcg ctaaactttc cggttatctt tatcaaaata tccaggtgga
    48781 tgaaaatggt gcaggaagaa taatccttcc gcaagaaatt cttgataaat atggaatcga
    48841 tgattcagag acagcttcga tcgtttcact tcctggagtg attgacgaag tgctggcttg
    48901 ggggattttt gttcagcagc cttcagggta ttaccgcgta cgtttacgtt ctaaggggcc
    48961 gatcatcaat gaattggcta aacgccatca tggaggaggc catccattag caagtggtgc
    49021 gaatgcaaaa gatttagcag aagtagagga aatctataaa gagatccaag atatatgcca
    49081 atcgtataag agcagaaaaa cagaatgata cttcgttcga aaacgtgcct tttgtagtaa
    49141 aaatcacaaa tgtatctttt caagatcttc tttttccgtt acaatagtgc tcactaaaga
    49201 ttttaaagga gaaagaagat gacattacca aattgcccag aatgtggctc agagtacaca
    49261 tatgaagatc gaggcttgtt gatttgccca gaatgcggga acgaatggaa caaagaagaa
    49321 caagaaactg tcatagaaga cggattagtg gtattagatg caaatggaaa ccgtctgtta
    49381 gagggcgata gcgtgacagt gattaaagac ttgaaagtaa aaggtgcgtc aggtgccatt
    49441 aaacaaggaa caaaagtcaa gaacatccgt ttagtagaag gcgatcataa tatcgattgc
    49501 aaggtagaag gatttggacc gatgaaactg aaatcggaat ttgtgaaaaa gaattaaaga
    49561 atattcaagg tcttttcaca ttcttttcct actactaatg ataaaataaa gtaaacgaaa
    49621 aggagtgaaa cgaaaatgta tggttatgga atgtatggtt ttttagatcc gacatttctt
    49681 ttggtcattc ttggattagt tatttccgga attgcgtcag cgtacgtcaa tagtactttc
    49741 cgaaaatacg atcaagtgag aagcaaaaac aatgtgactg gtacacaagc agcacaatat
    49801 atattacaaa gtcaacagat aaataatgtc ggtgttcaac aaattgccgg agatctcaca
    49861 gataactaca attcaggtaa taagatgttg agtttgtctc aagcaactgc acagtctact
    49921 tcagttgctg cgataggtgt agcagctcat gaatgcggac atgcggtaca agatgcttcg
    49981 ggttatgtcc cattgaagat tcgtgcagca cttgttccag cagctaactt tggttcgatg
    50041 atttcgttcc ccttgattat ggtaggtgta ttgcttagct ggaactctac gttaatcaat
    50101 atcgggattt tcgctttttc tttagcatta cttttccaat tggtgacatt accagtcgaa
    50161 tttaacgcgt ctcggcgagc aatccaaatt ttgagtgaag gcggactact gacggaagaa
    50221 gaagtaccga tggcaagaca cgtattattt gcagcagcac tgacttatgt tgcagcagcg
    50281 ctttcgacat tcctgcagct gttgcgcttg atcttattgt tcggcggcaa ccgaagagat
    50341 taaaataaag aagttgtgaa atcagcgctt tgatctgagc taataccgag agttagctga
    50401 ttgaatacca tttatttgga ataagaggat gtgacaagaa ttttgtcaca tcctctttgt
    50461 tttgttctca taaccttttt tcatatacag ttcatgaacc gcttttatta acttgccaaa
    50521 taacactgta ttgtgcgttg tatccagata gaaagtctcc gtttcatctg aaattttctc
    50581 atgctgatct gtaatgatca aatcagcatg ttctgaatat gcggtaattt taacagcatc
    50641 tttgcgaaat aactgctgca aatggctttg aagatgataa tttcctgtaa actgtttggt
    50701 tatctgcaaa tggatgagta actgctcttt cgtttctgtt tgtagtaagt gttggatcaa
    50761 ttgactaagg tattgactga tttggtttct ttccccattt tttgaaaaaa ttgtatcgat
    50821 tttttctctt aatttctttt cctgtataaa agaactagaa tgattggacg tggcttctgg
    50881 aaagcaaagt gtagaaaaac tactgctgga ttcacccaac agttcgaaaa aaacaactgc
    50941 caacacaaga aaataaaaat aaattggtat tttattttct aaatggttga gaggaaactg
    51001 ttgagttaag taagtcatca attgaccgac ttgtcgacaa ataaggtttt tgcttgtgga
    51061 aaataaaaaa ccaatctcct gtttttgtcg aaaagagatt tgatttggaa aaaaacaata
    51121 gaaaaaataa ttaaagtagg ctctctctgt ttctgtctct tcacaagggc gactcactga
    51181 aaaagaagta aagtattctg aataatcatg tgtatgctgc aataagtcca tcaattcagt
    51241 gatttcagaa gaagtttttg gaaaatatgc ctgttgtttg tttcgtaata tgaataaagt
    51301 taacagcaag tagatatttt tttcttgttt tgaaattttt tctatttgct catctgtact
    51361 gttcattttt agtgcattta attcaagtga ggaaaatggc caatcgttac ctccaaatgt
    51421 atctgaaaga aaaaagtaaa gatagagacg gatagataac tcatttcctt caagagtaca
    51481 gcaatttttt acagatagga tccgtaaatg gaattttatt aaggaatgat tgagctgttt
    51541 tgtcagtttt tcgagatagg agggcgatat ataaagttgt tcaagtaaat atttttttgg
    51601 tacagcagtt tgtggataat tgattagtaa catcaaatag ttaaaacgat tggattcttt
    51661 taggtagcat aattttaact tagaaaaaac agctaacttt ttagtttgat cggtttcttt
    51721 ggtttggatc tctcctttat gattagaata aataaattca tcaatcaata aaaattgctt
    51781 gagtgataca tttatttttc ggatcagttc tttgattgtc cgttttggaa tatgtaagtc
    51841 attggataat tcttgtaaag aacaattatt tttttgaatg accatttgta gaacaagtaa
    51901 ctcttttttt cttgcttcaa ttagcataaa tacctccttt ttattctgtt tttgtatata
    51961 gttagacaaa aactgtgagt ggaaagtcat ttcagtctgt gttattctca cagagtgtcg
    52021 ccttcttttt tatataacgt tatttaattt ttttgttacc ttgtttattc tattgtttat
    52081 ttcctagggt attttgtcaa gatattcttg tttttaaggc ggaaaaattg atttttaatg
    52141 gatataacta aatatgtttt gataattatc aataatattt ttagaattat ttatgttatt
    52201 tatttatggg tagatgaaaa gagacggagg taagaatgat ggggagagta acaaattata
    52261 gtaaagaata tttgctgttc aaaagtatgg tatacgtgga agaatatgga atgaagtcaa
    52321 ttactgcaag agagttagca gagttctgcg gttgttctac atacccgatt tatacacatt
    52381 tcaaatcaat cagcgggtta aaagagaaga tattagaaga aatcactgtt tattttgaaa
    52441 actacttagc tgaatgcagt tccgatgatg ttttaagcat tgtctactta ttgaaagatt
    52501 ttttttctat ccatgaaagt attcgtgaaa tcattgcaaa atctgattta gacatggatt
    52561 cgctatttaa gcggccattt ttctcatatg tcaaaggtca cacgaagatc aaagaagaaa
    52621 agtcgcttga attggtatgg cttaatgcat tgggaagttt gcgctcagat gattcttctc
    52681 aagattttct tgaaatgatg aacgctgttt gggataaaaa cgaggtgacg gatgaatact
    52741 tggaaaatat cttaaaggaa cagtcacctt aggcagcaac atgaaaaagt agatcagcca
    52801 cctttatttt tcgtaaaaaa gattgtaaaa tgtctaacga aacattggaa tgaaggagga
    52861 agcgacaacg ttaccacagc aatttatcaa aaaatatcgt cttcttttag gcgaagaagc
    52921 atccgatttt ttttctgcgc ttgaacaagg aagcgtgaaa aaaggatttc ggtggaatcc
    52981 actaaaaccg gcaggattgg acatggtcca aacttaccat agcgaagaat tacaacccgc
    53041 tccctacagc aatgagggat ttcttggcac tgtcaatgga aagtctttcc tccatcaagc
    53101 tgggtatgaa tatagccaag aaccaagtgc aatgatcgtt ggaacagctg ctgcagcaaa
    53161 acccggtgaa aaagttttgg atttgtgtgc ggctcccggt ggtaaatcaa cccaattagc
    53221 tgctcaaatg aaggggaaag ggttattagt gacgaatgag atctttccga aacgtgcgaa
    53281 gattttatca gaaaatatag aacgatgggg agtttcgaac gcaatagtga caaatcatgc
    53341 acccgcagaa cttgtccctc atttttcggg cttttttgat cggatcgtgg ttgatgctcc
    53401 ttgttcagga gaaggaatgt tccgaaaaga tccaaatgca ataaaagagt ggacagaaga
    53461 atctccgcta tactgtcaaa aacgtcagca agagattttg tcttcagcaa taaaaatgct
    53521 aaaaaataaa ggacaactga tttattcgac ttgtacgttt gcaccagaag aaaatgaaga
    53581 gatcattagc tggcttgtag aaaattatcc agtaacgatt gaggagattc ctttgactca
    53641 atccgttagt tcaggacgtt ctgaatgggg aagcgtagct ggtttggaaa aaacaatacg
    53701 tatatggcct cataaagatc agggtgaagg acattttgtt gcaaagctga cttttcatgg
    53761 acaaaatcag atgcataaag aaaagaagac acgaaaaaaa agtaaagtgc aaatgacaaa
    53821 agagcaggaa aaactctgga cggagttttc aaatgatttt cattatgaag ctactggaag
    53881 acttttagtg ttcaacgacc atctttggga agttcctgag ttagcccctt ctctcgatgg
    53941 tttaaaagtc gtacgtacag gtttgcattt aggagatttc aaaaagaatc gatttgaacc
    54001 tagctatgca ttagcgcttg ctacaaaaaa gatagagaat ataccttgct tgccgatcac
    54061 tcaaaaggaa tggcaatctt acacagcagg agaaacattt caacgtgatg ggaatcaagg
    54121 atgggttttg ctagtattag ataagatacc tgtcggattt gggaaacaag taaaaggaac
    54181 agtaaagaac tttttcccta aaggattgag atttcactga gtggattagt tcgtttattt
    54241 ttttgtctat gctatgatgg agatagataa aaaggagctg atacttatga aaacatcaac
    54301 aaaagtaaca atcggattaa gcgtagcagc cgctgccagt gtagcgacag cagtcgttgt
    54361 atcgggaaaa gtaatggaaa agatccatca tatgactact cggacgaaag taaaaaaatt
    54421 cgtctatgac aaatttgatg gaaatgaaaa attactagaa atcgtagatg gtctttcaga
    54481 tagtgacttg gattctttga tgggaatgtt agcaaaaatc aaatcaggaa aaaagaaaat
    54541 ctctgtttat ggtgattcaa tcaaagattc aaccgaagat gtaaaagacc ggctaatgca
    54601 ttttgttgaa aaaatgatgt aagagaaact agcttttgaa cactattcat tcgaaataag
    54661 gggttgtgac aaaattattg tcacaacccc tttaatctat ctttaattat gttcatgaag
    54721 caataaaaga tagcaaaaca gtaaatcgtt ggcttttttt agattgaaac cagactgttc
    54781 ttgaaatttt tcgattcgat attttagagt attacgatga agaaacagtg tttttgcagc
    54841 agagctgaca ttgcctaatt cattccataa ttcttgaatg atcacttcca tttcattttg
    54901 tttgatcagt tctatgtaac ttttgatcaa cttactatct tttacagtat cttttgtata
    54961 gtaatgtaaa gccacatctt gcatagaaaa agtacggctg tgagtataaa gattgttttg
    55021 ctctgttaaa aaaatttttc tttcttcagc aaacaaaggg acgaggtcgg tgccaggtga
    55081 atggaaagaa ccaacgaata cagacgttgt cgtatcaaaa tcagcatcta aagatagaaa
    55141 aataccgttg atttcagtag catcaagata atcacggttt atttgttcga ctaagagcgc
    55201 atctgtgtct gtataaaaaa agaaatcagc agaatagcga aataattcca aaatagtatc
    55261 ttgccattct ttttttaaga attctccttt tgattcgacg tgaagctgga tcaaacgaaa
    55321 gcttttttct tgaacacaag aagcatcttt aaaaagatag tgataccatg ggtgtttttt
    55381 ttgatcgttg attacaggga ataacgcttt caaaaggctg gtttcttgtt gagataatga
    55441 atcttgtttg atccagataa aagcatcatc aattgcaaaa gaaaggatat ccgcttctga
    55501 tgctggagag ttttttttct gtgcttgtgg gtaaagagca agtaatttat ccattttcat
    55561 cttgttcacc tcttgtattt attttaccat agaaaaaaaa gttagttaca gcctttttat
    55621 catatgctaa agtaaaacaa gaaaaaacaa aggcagggaa gggtaattgg taaatacaaa
    55681 tcttgatgca gtagaaaaag aaaaatggat gcgccttgca ttggcagaag caaagaaagc
    55741 agaaatgctt catgaagttc cgattggagc agtggttgta ttggatggaa aagtgattgg
    55801 aagaggatat aaccttagag aaaccacgca agatgctact acacatgcag aaatgcttgc
    55861 aatcaaggaa gcctgtgaaa aagtgggaag ctggcgcttg gaagatgctg cactttttgt
    55921 taccttagag ccttgtccta tgtgcagcgg cgcgatgatt ttgtctcgtg tagcagaagt
    55981 ttatttcggc gcgtatgatc caaaaggcgg aacagccggt accttgatga atcttttgga
    56041 ggacgagcgg tttaaccacc aagcatatgt agaaggtggc attttagaag acgaatgtgg
    56101 gatgattttg actgactttt tccgaaagct ccgccagcga aaaaagaaat taaaaaaaga
    56161 ctggtcaacg aatgaaaaat aaggtatact attctttgcc gaaaggctag gacaatggtg
    56221 ggcttgtgaa gcgtgtcagg tctggaagga agcagcacta agcaggttcc gccatgtgtc
    56281 tgattataat gaaacgacaa cagactttcg ggtctgtttt ttttattttt atagtactgt
    56341 aaaaatgaat gttcctgatt ttttagccag actgatttat cgaatattgt acttatacaa
    56401 tttgcttcaa ggcaggaaaa actctgaaat agagcttgtt tcaatggaag aaaaagctgc
    56461 ctatggtatg ataaagataa agagaggcga gagggtacat ggaaaaaata gtgattttac
    56521 atacgaatga tctgcattct catttagaaa attggccaag gatcagaagg tttttggatc
    56581 aacgtaaacg agaaaacgaa aagaaagaaa aaacgacaac gattgcagta gatctaggtg
    56641 attttgtgga tcgttggcat ccattgtcag aagcaacgaa tggacaggaa aatatcttgc
    56701 tgatgaatga agttggttat gatgcagcga cgataggaaa caatgaaggc gtaggaaatt
    56761 ccaaagaaga gttgaatcat ttatacgatc atgctgagtt tgatatcatc ctggataact
    56821 tattcgataa aaatacgctc cagccaccgg catggacgaa accatataaa atcattacaa
    56881 cgaaacacca gacgaaaatc ggactaattg catttactgc tccatttccg cttacttata
    56941 atccgaatgg ctgggacata cggaatcctt atgatatttt gccagaatta gtcgaagatt
    57001 tacgttcaca agtggatgtt ttagttttga tgagtcatct cgggattcag gatgatttcc
    57061 aaattgcaaa agaaatacca gcaattgatg ttattttagg atcgcacacc catcatttat
    57121 tcagagaagg tcacgtaatc aatgatgtcc aaatagcagc cgctggaaag tacgggcagt
    57181 atgtcggtga agtccattta accatcgatg aagagaaaaa aatccttaag cattcagcca
    57241 aagccatccc taccgaaaca atgacagctt ttacagaaga tgaacaagaa ataaatagtt
    57301 atctagaacg tgggcatgaa ttattaaaag aaaaagaagt agcagatttg ccattttctc
    57361 tgagtctaga tatcttcaat gagcattcgt ttatccaagt agcctttgag gcggtgaaag
    57421 aacgaggaaa gacggaagca gcaatattaa acagtggtct gtttttaact gaaattccaa
    57481 aagggcgggt caatcaggat cagcttcaca ctgcgttgcc gcatccgatg cacttgttaa
    57541 atgtcacatt gggtggaaat gatctgatcc gcttagtttt agaaatagaa aaaaatcgta
    57601 actttttacg taactatcct atgaagggaa tgggatttcg cggtaagatt tttgggcaga
    57661 tggtctataa tggaatcaca tatgattcta ttaaccatca agtcttttgg aagaacaagc
    57721 caatcgatct gaataaaaat tatactttta ctaccgtaga tcatttgatg tttgttccat
    57781 tttttccaac gattgagatt gctggaaaaa atgaattttt gttccctgaa tttatccgaa
    57841 gtgtggtagg agattacttg aaagctcact acccaatcaa ataaaaacaa ggtataataa
    57901 aaaagatagg tggtgaacgc atgtctgcaa aacaaaatga acagcagaac gttgagcata
    57961 aaacagaaat tgctaagaaa aaatcatcag ctgaacagga aacaacagtc accgaagaac
    58021 tgacagctgc tttggaagaa atcgttgagg agcctaaagc agaaaaagtc aaaggcgagc
    58081 tagtgacact gttagatgac agtaattttt tgataggtga aagacattat cggcttgtgt
    58141 ccgattatcg tgaaggattt aatgcagaaa agcttggaga acgttacagt gatgtactag
    58201 cccgatatga ttatatcgta ggagattggg gttatgaaca actacgccta aaaggatttt
    58261 tccaagcgga taatcgacgg gcgcatccag atcagcgaat cgattccttg gaagactatc
    58321 tttacgaata ttgcaatttt ggttgtgcgt attttgtcat cgagagaatc ggcggtaaaa
    58381 aagaaaagac aactcagcgc cgaaaaaaga aaaaaaacca taatcgtgcg tatatcgatg
    58441 agaaaaaagg accagtcgca tctaatcgca aaaaaccggt tattaaaaat cgaaaaacag
    58501 agtcagcaaa aacctctgaa aaagtaaaaa aacaagaaca acctaaacaa gtagaaaaaa
    58561 caaaaacagc atttacgatc agaaaaaggg aagagtagtg gatgaatgga ctataaagga
    58621 tatttaattg atttagacgg aacgatttat cgtgggacac aacccatacc ggccggtaaa
    58681 cgatttgtgg aagaattgca aaaaagaaaa ttacctttct tgtttgttac caataacacg
    58741 actaagtcac cagaaacagt agctaatcgt ctagcagatg agtttgacat tcatgtagct
    58801 ccagagacag tctacacagc tactttggca acaatcgatt ttatgaaagc agatggaaaa
    58861 gggaaaaaag tctatgttat cggagaagca ggattgattg acttgatttt agctgccgga
    58921 tttacatggg aagaagaggc tcctgactat gtagtggtag gcttagataa ttatctgacc
    58981 tatgaaaaag tagttaaagc aacacttgct attcaaaaag gtgctacttt cattggaacg
    59041 aacccagata agaatatacc tactgaacgc ggccttcttc ctggagcagg gtcagtcatc
    59101 tcttttgtag agacagcaac ccaaacgccg ccgatctaca tcggaaaacc agaagccatc
    59161 attatggata aagccgttga agtattaggg ctgcaaaaag aagaagtaat catggttgga
    59221 gataactatg aaacagatat ccaagctggg atacgcaata atatagatac tttgctcgtt
    59281 ttatcaggtt ttacaaaaga agaagatgta cctgggttgc ctatcccagc tacctatgtg
    59341 aaacaatcac tggatgaatg gagcttctaa tatgaaaaat tcgagatggc agtggatgga
    59401 atatgctgga ttgttcagcc tatttctgac actgatcagt ctggcagttg gtgtgacgat
    59461 caactttcga ccactatacg tatttgatat cggacatttg caaattttgg attatacttc
    59521 tttagatcaa gagacgttac tgaaaaattt tgatcaccta atgaattatc tcaataatcc
    59581 cttcaaaacc atcctcagct tgccagattt cccagtttct gctagcggag cccatcactt
    59641 ttatgaagtg aaaattttgt ttttagtgga ttatgctgtg tttttcatca ctttgatccc
    59701 gagtattttg tttatcaaat atttgcaaaa gaatgatcgg ctttggcggt tgattcgtcc
    59761 gtttcaaatc ggcatgttac tgccagttgt tttcggtttt ttcatgatga taggatttga
    59821 tcgctttttt atcctttttc atgaaacgtt tttcaacaac gatgactggt tattcgatcc
    59881 cgttactgat ccaatcatca atgtacttcc agaagaattt ttcatgcata gctttatctt
    59941 attttttgta ttattagaat tattttttgc tgtttttctt ttcttaggaa aaaatagctt
    60001 gaaacaaaca aaaaagaaag aactcgtcta aatagacgtt ttctttcttt ttttgttgaa
    60061 ttattgagta aggcgacttc ttttttctgg ttctttgtgt gctaatcgac tggcagcagc
    60121 agcggcgatc gctccgacga tatcatctaa gaaggtatgg acatgaacgc catcgtggct
    60181 gtttagttct tttaaaatac caggtttcac cttgtcgata taaccataat tcgtaaagcc
    60241 gatcgtaccg taaacattca cgattgacaa ggctaggatc tcatcgatac cgtaaaggcc
    60301 ttcgtcatct tcgataatat gttgcaacgg actcatcaat tttttctttt ctgctaatat
    60361 atctaattgg attccagtga taatggcgtt atgtacttca cgctttgtta gcacgctgtt
    60421 gacactttca gtacaatccg ccaaagtcaa atcagaaata tagtcttttt gtaaaaacat
    60481 gacaagttgt gcgatatctt ctacagttac gccacgttcc ttcaatagtt ctcttgcctt
    60541 ttcttctaaa gtctcagttt tgacgaccat attattgctc cccctttatt ttttctttct
    60601 ctatttcatg ttaacggaac atgaaaaaaa tgcaagaaac tgctttcggg tttaacttga
    60661 aatgcaaaga gatataaaaa atcaactagg acgaagattt ttatcgtata agcttgtgct
    60721 gtgaccaggc tgagttcgtt cctttggatt aatatagtgt aaagcgttgt tcactgcagt
    60781 aggtgcttca ccgagcccag tagcaatcag tttcactttt ccttcatagg tacagatgtc
    60841 accagctgca taaacgcctg gaatagaagt agacatatct gattttacag taatggcatt
    60901 tctggtacta tcaagtcccc aagaagaaag atgttctaaa ttagaagaga atccataatt
    60961 gacgatcaga gaatcaatca tcaaatcaat cgtctcgtca cttttcactt tttttaaccg
    61021 gatatctgtc agttccccat tattgcctga taatccatcg atgagatagg gagtaagcaa
    61081 gttgacagat gaagatttca agcgtgatac actatgttcg tgcgcacgaa attccgggcg
    61141 acgatgaatc aagtaaactt cgcttgcaat aggctctaac atcaacgtcc aatcgatagc
    61201 agaatcacca cctccagcaa tagcgacttt ttttccagca tacttcatca aatcattcac
    61261 gaaataatcc aatccgtggt tttcaaatga ttcagcatta tctaaattca attttcttgg
    61321 ctggaatgaa ccatttccta aagctaaaat gactgctttg gaataatgga tgcctttatt
    61381 tgtagtaatc tcgataactt cgtcttcacg agtaagcgag agaacttctt cttttaaatg
    61441 gaatgtatgg ttaaaagtag ttaattgttt ttctaattga tcgattaatt cacttgcttt
    61501 tattgctgga tacccaggaa tatcgtaaat atatttttcg ggatacaaag ttgctaattg
    61561 accaccaagt tgaggtagac tgtcaatgat ttttgtttta gcttggcgca ttcctgcgta
    61621 aaaagcggca aacatcccta caggacctgc accgatgatt gtaatgtcat agatttccat
    61681 aaaaattcct cccttttcac actatatcag aatttaattg agaatgcttc tcaaaaatgt
    61741 ttgaattcga aatcgaaacg gtaagttata gaatgtatct agtgatgaaa aataaaagaa
    61801 aacaaatcta gtatttttac gagaggttag gtattatgtt attattatta tgaagtatgc
    61861 aaatattagg aggaaacaat atggcattcc cacaactaga tttaaaaagt gaaaaaggac
    61921 caaaagctgt tatcaaaaca aaccgtggtg acattactgt tcaattattc cctgaactag
    61981 caccgaagac ggttcaaaat ttcattgaac tttccaaaaa aggttattat gatggcgtga
    62041 tttttcaccg cgttattcct gatttcatga tccaaggcgg cgatccaact ggaacaggaa
    62101 tgggtggcga aagtatttat ggcgaaagtt ttgaagatga attcagtcgt gaactgttca
    62161 accttcgcgg agctttatct atggcaaatt caggtccaaa cacaaacgga agccaattct
    62221 ttattgtaaa taacacaaat gtgccagcta atatgcttgg acaactagaa ggtgctggat
    62281 tcccatcaga aatcattgaa gcgtataaag gtggcggaac tccttggcta gatttccgac
    62341 atacagtctt tggacatgta ctagaaggga tggacacagt agatgaaatc gcaaatgtac
    62401 aacgtggtcc acaagaccga cctgtacatg atgtagtgat tgaaacaatt gaaatttcag
    62461 aatagtttgg tatggtacgg agtaattatg aaaaaggacg aaacctagta aggtctcgtc
    62521 cttttatttt gaataaatga tttaatcgat tgtctctaaa gcatacttga ttcgattaag
    62581 tccttccagt aaagtttctt ctggacaagc aatattcaag cgcatatgcc catgaccgga
    62641 aggaccaaac gtgatgccag gatttaatac gacttttcct ttccgaacaa gagtgttctc
    62701 caattcttgg tcgtctgaaa tataggcggt aaaatctaac cacattaagt aagttccttc
    62761 agggttcatt attctcactt tcggaagata ctgttcaaaa aactgattaa ctgtttgaac
    62821 atttttttct agataaggaa tcagttggct taaccattct ccacccgtct cataagccgc
    62881 ttgggttcca atcagtccaa aagtgttgat ttcgtgctgt tggttcatca ctaaatgttt
    62941 ttcaaaatca gctttcatct ctggattttt gataaatacc attgaatttt tgattgcagc
    63001 tagattgaaa gtcttggttg cggaagtaaa agcaactagt agatcatcta aattaggatc
    63061 aatcgttagc atggaagtaa atgtatgatc gaataaagtt aaatcttgat ggatctcgtc
    63121 actaaacaga atcacttgat gttttaaaca caattcgctt aactgtttca attcttcttt
    63181 gctccaaaca cgccctccag gattgtgagg attgcataaa atcattgctt ttacattgtt
    63241 ttcttcgatt tttttctcca tgtcagacaa gtccatgatg aaatgattat ttttttctag
    63301 caaagagctt cgaacgatgt gacgttgatt tgtttctata atagaggaaa atggaggata
    63361 aactgggtca tgaatcaaaa tagaatcgcc tggttttgta aaagtttgta ctgctgcagc
    63421 taaactggtt aacacaccac ttgtaaaaac gatattttct ttcgtcaact ccacagcatg
    63481 gtgttggcgt tcccaatgaa tgatagcctg atatagttta tcagacagag tggcataacc
    63541 aaagattcca tgtttgatgt attcactaaa cgctttagct accccaccgg gagacaagaa
    63601 gtccatatct gctacccaca aaggaagtaa gtcatccatt tgatatgtct cagcaattgc
    63661 gtcccatttt acactttctg tatcttttct tgaaatctgt ttatcaaatt cgatcatctt
    63721 aagacctcca gtcattttcg tcgcttcttt ttttgagcta tttacagtat aatgataaat
    63781 gcgaagagag ggaagtagat gacttataaa attggacaaa taattgaagg aaaagtgaca
    63841 gggatacagc cttatggcgc attcgtttct cttgataacg agacgcaagg acttattcat
    63901 gtttcagaag tacaatccgg ttatacgaaa aatatccatt cattgttaaa agtcggacag
    63961 ccagtaacag ttcagatcat cgacattgat gaatattcca aaaaaatcag tttatcttta
    64021 agaacgttag aaaaaacaaa tccagctgtt ccatatcgca gaaaaaggta tttcacgaat
    64081 aaaaatcgta agatcggatt taccactatt gcggagcagt tacctatttg gatagaagaa
    64141 gcattatctg aactagaacg gaaagaaaat aagaaataaa aagtgtattt tttctgaaaa
    64201 tgtgctagaa tttttatgta agccatagaa caagttgatc gaggtggaaa ttttggcaag
    64261 caaaagagtt gctggtacaa ttatgttgca tttggaggat ggtactaaaa aatttttggt
    64321 ccattcaatt gatgacaagt tggaatttgc gttagcggaa ctttctgaag gaaagactgg
    64381 tttagcgaat atacttaatt tcttaaaaga aatagttcat atcgatgtta gcaagattag
    64441 tttgatggaa ttgacaaata cacatattaa agaggaaaat gttccgttgt ttgtctttga
    64501 aactgaacaa aagaatcaaa gagaagaatt aggcgaagga tatatttggg aagaaccagg
    64561 tcaaatgcgc tctgttcttg gaacttacga agtagaggga gtgcctttct tctaaaatac
    64621 agattaagcg aacaaagaag tttgaatttt tttcaaactt ctttttttat tgtttggtcc
    64681 aaaaaataca atgaaatgtt ctgctaaaga tagtttttta gtgcatttta ttttcaaaat
    64741 atgttgaaaa cggttgtata acgaataata ataattgatc gaaaaatcat ttcacaaagt
    64801 aatgcttaat ttatagttga ccaaggaaga aatacatgat aaatttaaca gcgttgattc
    64861 tgaagtggat gttatatcac atttctaaaa aatttaaaaa atagttattg acatgttact
    64921 aacgacatga taagataact gagtcgttaa aaaagaacca cttgcgaagc tt
//
LOCUS       DACJGB010000006        63006 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.006, whole genome shotgun sequence.
ACCESSION   DACJGB010000006 DACJGB010000000
VERSION     DACJGB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.006"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..109
                     /locus_tag="IYI34_000419"
     CDS             <1..109
                     /locus_tag="IYI34_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0558320.1"
                     /translation="YIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            237..1451
                     /locus_tag="IYI34_000420"
     CDS             237..1451
                     /locus_tag="IYI34_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAR0558321.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            complement(2161..2349)
                     /locus_tag="IYI34_000421"
     CDS             complement(2161..2349)
                     /locus_tag="IYI34_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558322.1"
                     /translation="MAVREYHYLTVQVMAIKEDKSFTRTQIGFRSLLQGKGRGVFVST
                     ASCLSNVLNSFIQLSKND"
     gene            2440..2649
                     /locus_tag="IYI34_000422"
     CDS             2440..2649
                     /locus_tag="IYI34_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HAR0558323.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2761..4374
                     /locus_tag="IYI34_000423"
     CDS             2761..4374
                     /locus_tag="IYI34_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4368 domain-containing protein"
                     /protein_id="HAR0558324.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4409..5620
                     /locus_tag="IYI34_000424"
                     /pseudo
     CDS             4409..5620
                     /locus_tag="IYI34_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5643..6569
                     /gene="glsA"
                     /locus_tag="IYI34_000425"
     CDS             5643..6569
                     /gene="glsA"
                     /locus_tag="IYI34_000425"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAR0558325.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6777..8237
                     /locus_tag="IYI34_000426"
     CDS             6777..8237
                     /locus_tag="IYI34_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558326.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8458..10221
                     /locus_tag="IYI34_000427"
     CDS             8458..10221
                     /locus_tag="IYI34_000427"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAR0558327.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10305..10763
                     /locus_tag="IYI34_000428"
     CDS             10305..10763
                     /locus_tag="IYI34_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAR0558328.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10872..11555
                     /locus_tag="IYI34_000429"
     CDS             10872..11555
                     /locus_tag="IYI34_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAR0558329.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIIDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11702..12316
                     /locus_tag="IYI34_000430"
     CDS             11702..12316
                     /locus_tag="IYI34_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAR0558330.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12332..12538
                     /locus_tag="IYI34_000431"
     CDS             12332..12538
                     /locus_tag="IYI34_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558331.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13079..14209
                     /locus_tag="IYI34_000432"
     CDS             13079..14209
                     /locus_tag="IYI34_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAR0558332.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            14301..15056
                     /locus_tag="IYI34_000433"
     CDS             14301..15056
                     /locus_tag="IYI34_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAR0558333.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15114..17153
                     /locus_tag="IYI34_000434"
     CDS             15114..17153
                     /locus_tag="IYI34_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAR0558334.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQVTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17262..18413)
                     /locus_tag="IYI34_000435"
     CDS             complement(17262..18413)
                     /locus_tag="IYI34_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAR0558335.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(18567..19097)
                     /locus_tag="IYI34_000436"
     CDS             complement(18567..19097)
                     /locus_tag="IYI34_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558336.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(19196..19546)
                     /locus_tag="IYI34_000437"
     CDS             complement(19196..19546)
                     /locus_tag="IYI34_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAR0558337.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(19703..21232)
                     /gene="ahpF"
                     /locus_tag="IYI34_000438"
     CDS             complement(19703..21232)
                     /gene="ahpF"
                     /locus_tag="IYI34_000438"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAR0558338.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(21242..21805)
                     /gene="ahpC"
                     /locus_tag="IYI34_000439"
     CDS             complement(21242..21805)
                     /gene="ahpC"
                     /locus_tag="IYI34_000439"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAR0558339.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(21949..22611)
                     /locus_tag="IYI34_000440"
     CDS             complement(21949..22611)
                     /locus_tag="IYI34_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0558340.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            22795..24441
                     /locus_tag="IYI34_000441"
     CDS             22795..24441
                     /locus_tag="IYI34_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAR0558341.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            24432..25514
                     /gene="uxuA"
                     /locus_tag="IYI34_000442"
     CDS             24432..25514
                     /gene="uxuA"
                     /locus_tag="IYI34_000442"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAR0558342.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(25622..27175)
                     /locus_tag="IYI34_000443"
                     /pseudo
     CDS             complement(25622..27175)
                     /locus_tag="IYI34_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            27323..28464
                     /locus_tag="IYI34_000444"
                     /pseudo
     CDS             27323..28464
                     /locus_tag="IYI34_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(28590..29669)
                     /locus_tag="IYI34_000445"
     CDS             complement(28590..29669)
                     /locus_tag="IYI34_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR0558343.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(29772..30212)
                     /locus_tag="IYI34_000446"
     CDS             complement(29772..30212)
                     /locus_tag="IYI34_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0558344.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(30269..30679)
                     /locus_tag="IYI34_000447"
     CDS             complement(30269..30679)
                     /locus_tag="IYI34_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAR0558345.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(30859..31293)
                     /locus_tag="IYI34_000448"
     CDS             complement(30859..31293)
                     /locus_tag="IYI34_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAR0558346.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(31393..32241)
                     /locus_tag="IYI34_000449"
     CDS             complement(31393..32241)
                     /locus_tag="IYI34_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR0558347.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            32630..34396
                     /locus_tag="IYI34_000450"
     CDS             32630..34396
                     /locus_tag="IYI34_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAR0558348.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            34498..35757
                     /locus_tag="IYI34_000451"
     CDS             34498..35757
                     /locus_tag="IYI34_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR0558349.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            35904..37205
                     /locus_tag="IYI34_000452"
     CDS             35904..37205
                     /locus_tag="IYI34_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0558350.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            37208..38059
                     /locus_tag="IYI34_000453"
     CDS             37208..38059
                     /locus_tag="IYI34_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0558351.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            38072..38887
                     /locus_tag="IYI34_000454"
     CDS             38072..38887
                     /locus_tag="IYI34_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAR0558352.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            39038..40204
                     /locus_tag="IYI34_000455"
     CDS             39038..40204
                     /locus_tag="IYI34_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0558353.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            40434..41081
                     /locus_tag="IYI34_000456"
     CDS             40434..41081
                     /locus_tag="IYI34_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAR0558354.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            41165..42109
                     /locus_tag="IYI34_000457"
     CDS             41165..42109
                     /locus_tag="IYI34_000457"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAR0558355.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            42244..43695
                     /locus_tag="IYI34_000458"
     CDS             42244..43695
                     /locus_tag="IYI34_000458"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAR0558356.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            43716..44552
                     /locus_tag="IYI34_000459"
     CDS             43716..44552
                     /locus_tag="IYI34_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAR0558357.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            44552..45211
                     /locus_tag="IYI34_000460"
     CDS             44552..45211
                     /locus_tag="IYI34_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR0558358.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            45337..46026
                     /locus_tag="IYI34_000461"
     CDS             45337..46026
                     /locus_tag="IYI34_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558359.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            46032..46988
                     /locus_tag="IYI34_000462"
     CDS             46032..46988
                     /locus_tag="IYI34_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAR0558360.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            47032..47544
                     /locus_tag="IYI34_000463"
     CDS             47032..47544
                     /locus_tag="IYI34_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0558361.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(47800..48348)
                     /locus_tag="IYI34_000464"
     CDS             complement(47800..48348)
                     /locus_tag="IYI34_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0558362.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(48437..49618)
                     /locus_tag="IYI34_000465"
     CDS             complement(48437..49618)
                     /locus_tag="IYI34_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAR0558363.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(49854..51362)
                     /locus_tag="IYI34_000466"
     CDS             complement(49854..51362)
                     /locus_tag="IYI34_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAR0558364.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            51521..51712
                     /locus_tag="IYI34_000467"
     CDS             51521..51712
                     /locus_tag="IYI34_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558365.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            51915..52832
                     /locus_tag="IYI34_000468"
     CDS             51915..52832
                     /locus_tag="IYI34_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAR0558366.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            52825..53898
                     /locus_tag="IYI34_000469"
     CDS             52825..53898
                     /locus_tag="IYI34_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558367.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            54046..54564
                     /locus_tag="IYI34_000470"
     CDS             54046..54564
                     /locus_tag="IYI34_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558368.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            54545..54937
                     /locus_tag="IYI34_000471"
     CDS             54545..54937
                     /locus_tag="IYI34_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAR0558369.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            55013..55948
                     /locus_tag="IYI34_000472"
     CDS             55013..55948
                     /locus_tag="IYI34_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAR0558370.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            56081..56860
                     /locus_tag="IYI34_000473"
     CDS             56081..56860
                     /locus_tag="IYI34_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAR0558371.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            56853..57170
                     /locus_tag="IYI34_000474"
     CDS             56853..57170
                     /locus_tag="IYI34_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAR0558372.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            57414..59024
                     /locus_tag="IYI34_000475"
     CDS             57414..59024
                     /locus_tag="IYI34_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR0558373.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            59181..60029
                     /locus_tag="IYI34_000476"
     CDS             59181..60029
                     /locus_tag="IYI34_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR0558374.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            60260..60739
                     /locus_tag="IYI34_000477"
     CDS             60260..60739
                     /locus_tag="IYI34_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558375.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            60772..63000
                     /locus_tag="IYI34_000478"
     CDS             60772..63000
                     /locus_tag="IYI34_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAR0558376.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
BASE COUNT        21118 a        11078 c        12408 g        18402 t
ORIGIN      
        1 gtatattcct ataaaagata ttatcctaca ctttgaaaaa tacgattatc gttttcattg
       61 gaatagtgat aattcagtta ttgaaatttg gatacctatt caagattaga agcaatttta
      121 tatccataca cacaaagcag ttagtcttag gattgactgc tttttctata cagattttta
      181 atgacaggca attatcaatt atggtagttg ccttttttga ttggaggaat tgaagaatga
      241 atgataaaaa ctttattgaa gaactaagac aaaagcgtga ggaatacgga gtaacacaaa
      301 caaggcttgc tgttgcctgt ggtatcagcc gtgaatatta caaccgcatt gaaaaaggaa
      361 aacagccatt gaatgacgaa ttaagagaaa tcatagaaaa acagattgag cgtttcaatc
      421 cgcaagaacc actattctta ttgattgatt actttcgtgt ccgctttcct actacggacg
      481 cattggcgat tatccgtgat gtattacaac tgaaatctaa ttacatgctt tatgaagatt
      541 atggaaaata cggatatgaa agcaaatatg tacttggcga catcaatatc atgtgttcca
      601 tgcaggagca tttaggtgtt ttattggaac tgaaaggcaa aggctgtcgg caaatggaat
      661 gttatctgct ggcacaggaa cgctcatggt atgacttcat gctggattgt atgacggctg
      721 gtggtgtgat gaaacggctt gatttggcta tcaatgataa agcaggaata ttggatattc
      781 caaagttaaa ggaaaaatac aaggctggcg aatgtatgtc ctacttccgt atgcagaaag
      841 attacagcgg tacagaaaaa tgcggtagcg atttaccaaa gaatacagga gaaaccttat
      901 atctcggttc aacaagtagc gaattatata tgtgtgctta tcagaaaaat tatgagcagt
      961 atgtcaagaa tggcacagaa attgaagata cggagattaa gaaccgtttt gaaatacgca
     1021 tgaagaacga acgagcctat tatgcggttg tagatttact gacctatcgg gacgctgaac
     1081 gcaccgcttt ttctatcatc aatcattatg tccgctttgt tgacagagag gacgacaagc
     1141 caaaaagtca atggataaca aatgatgatt gggcatggtt tgtaggggaa aacagagagc
     1201 cgatacgctt aaccacaaag ccagagcctt acactttaca aaaagcgttg cattggctac
     1261 aaagacaagt cgcaccaacc ataaaaatgg tacaggcatt agacagagaa aatcgtacaa
     1321 cgatactgaa agatatgatt gagcaggcag aactaaaaga caagcataaa catttattac
     1381 aattagagaa atcaaccata gaagaacgta tagataccgc tgttccacaa gagaatgacg
     1441 gtatttttta atgtaattga aaaatttcag atttagtcag caaagagctg ttttcattcc
     1501 ctatgtgaag tgaaagaaga aaaataaatt ttttattaaa tctgcaacaa aatggctact
     1561 tgcgtacaga tatggtgcga gaaaaggaaa tctaaacttt ttttgaaaat aggtcattaa
     1621 atcggggctt gctgtcccta tatgaagtga aagaagaaaa aagttttgaa aaaatgacgg
     1681 aaattcgtga ttagtggaca gtcttctgtg aaaagaggaa aaacatcatc attttgtatt
     1741 caaacaggca ttagcagttg agtgttgatg tgaaaagatg aaaatacttt gttctcaact
     1801 taacaattta aggtttccgt tccctatatg gtgcaaagaa aatatttcat tctattgttg
     1861 gcagtaacgg tatggtttgg gtagttaggg tgtgaaaagg aaaatattct tttttcatca
     1921 tcaacttagc aaaatgacat tttctatccc tatatggtgc atgaaaagaa gttaaaattt
     1981 ttccatttaa acttagcaaa tggacgtttg ctgtacagat agtagtgaaa aaggagaaaa
     2041 tcaaccgttg attgccaaaa tgaaccgttc catttgtact aataatggaa aaagttttgg
     2101 attttggtta cgtttctcct cttttccaac gcgatatata ggaaagacga tttttttctt
     2161 tcaatcgttc tttgacaact gaataaagct attcaatacg tttgacagac agctagccgt
     2221 tgaaacaaat acgccacgac ctttcccctg caagagcgag cgaaaaccta tttgtgttct
     2281 tgtaaaagat ttgtcctctt ttatcgccat gacctgtact gtcagataat gatactcccg
     2341 tacagccaca gcttgagcat taagagcgtc gcacgcaatg ggtacggcta cataagaact
     2401 atgcagaggt ggaactcctg tgggctatga caaaagccgt tgaattgctt agaaagattt
     2461 tacagaaagg gggtatgtgt tattgataat gctgtaaaaa caattcaaaa gaaaaatggc
     2521 aagtgtggca ttggcaacag aggaaagaca aagattgtag taaaagagca tttttccgaa
     2581 aagggcaaaa caatggaaga acttctaact gatgtcatgc tggaaaaagc aaagcagaca
     2641 atcgcataaa atcggtgcag ttgtaagaaa tagattgaat gacaaaaagt acccgtgtta
     2701 tactttactt gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca
     2761 atgaaacaac agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta
     2821 cagggcgaaa gttccagcat taccacacag agaagtatgt tgcgtttata tgcaaaagaa
     2881 catcatttga atgtcattga tgaatatatt gatgacggct ggtcgggaac aaattttgac
     2941 agaccgagtt ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta
     3001 acgaaagacc tttcccgtct tggcagaaat tatattatga caggacaata tacagaattg
     3061 tatttcccta gtcataatgt ccgctacata gcgattgatg acggtgtaga cagcgaaaaa
     3121 ggcgaaagtg agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca
     3181 agccgtaaag tcaaatcagc atttaagaca aagtttgcgg agggggctca ttacggtgct
     3241 tatgctccgt taggatataa gaaacaccct gacatcaaag gaaaactgtt ggttgatgat
     3301 gaaacaaaat ggattattga aaaaatcttt tccctagcct atcaaggtta cggaagtgca
     3361 aaaatcacaa agcagttaag agcagaaaaa gttccgacag cgtcatggct aaattttaca
     3421 agatacggta cttttgcaca tatctttgag ggaaaacccg aaagtaagcg ttatgagtgg
     3481 acgattgctc atgtcaaggc gatattgaaa agtgaggttt atatcggaaa cagtgttcac
     3541 aatatgcagt ctacggtatc gttcaagagc aaaaagaaag tgcgtaagcc cgaaagtgaa
     3601 tggtttcgag tagaaaacac tcacgagccg attattgaca aggaagtgtt ctatcgtgtg
     3661 caggagcaga taaaatcaag acgcagacag acaaaggaaa aggcaacgcc gatttttgca
     3721 gggcttgtca agtgtgcgga ttgtggttgg tctatgagat ttggaacgaa taagacaaat
     3781 aaaacgcctt acagttatta tgcttgcagt tactacgggc agtttggcaa aggtaattgt
     3841 tctatgcact acattcgtta tgatgtgctg tatcaagccg tattggaacg cttgcagtat
     3901 tgggctaagg cagtacagca ggacgaagaa aaggtattga acaagataca gaaagctggc
     3961 aatgcagaga gaatacggga aaagaagaaa aaggcaagta cattgaagaa agccgagaac
     4021 agacaaaatg agattgaccg tttatttgcg aaaatgtatg aagatagagc ctgtgagaag
     4081 ataacagagc gaaattttgt gatgttgtcc agcaagtacc aaaaagaaca gatagaactg
     4141 gaacagcaga taacaagcct aagagaagaa ctaagtaaaa tggaacagga tatgataggt
     4201 gctgaaaagt ggattgagtt aatcaaggaa tattccgtac caaaggaact gacagcacca
     4261 ttattaaatg ccatgataga aaaaatcctc atacatgaag caacaacgaa tgaggataac
     4321 gaaagaatac aggaaattga gatatattac cgatttattg gaagagttga gtaatcaaca
     4381 agagtaatat ttttaactaa gggaaaccgg cggtgtctat acttgggtca gtcgcacatt
     4441 gggaaaacga ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta
     4501 cttgaccatg ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat
     4561 acaaaacaat aaatatctga aattggcaat cctattgatt attttctggg ctattttact
     4621 ctcacagtta aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg
     4681 tatcttgttg ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc
     4741 tccactacaa acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac
     4801 gctggttgtt tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca
     4861 tgcaaatgaa atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat
     4921 tctggcaatc attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca
     4981 tggtttgtct cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga
     5041 tccaaatctt gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg
     5101 agaaattgct tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg
     5161 attacttcct gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat
     5221 cgcaaatggg atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa
     5281 taatctttca tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt
     5341 tatgtttttc gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta
     5401 tcaggttcca ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc
     5461 actttttgct ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg
     5521 gatttatgaa acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat
     5581 ctacgcatgc cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc
     5641 aaatggaagt aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag
     5701 gacaagtggc ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg
     5761 cgatttatga tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg
     5821 ccatcgaaag catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag
     5881 aaaaagtctt tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga
     5941 atatggaaat caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg
     6001 caacaacctc gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat
     6061 ttatgaaaga gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg
     6121 aatcaagaac aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga
     6181 tcgaaggaga cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca
     6241 ccgctacagg catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga
     6301 atggcaaaca aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg
     6361 ctggtttgta tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg
     6421 gtgtaggcgg cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc
     6481 cggcactaga tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca
     6541 aagaactaga cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc
     6601 aaacggctca aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc
     6661 agtttctttt tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac
     6721 gatttacaga tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc
     6781 tggaaaatta catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg
     6841 aattccaaga aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga
     6901 tccttcacga tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa
     6961 gagagaagca taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac
     7021 agtttctgta cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat
     7081 tcacgagtac agaactggct gatcttgaat ttatttcttt gagtaaagta tatacaatca
     7141 aaaaaatcgt attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta
     7201 tcccagaatt tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag
     7261 gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag
     7321 agactcattt ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgagaaa
     7381 tagagatcgc aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa
     7441 gcgcagaaac gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa
     7501 ttctagactt acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt
     7561 ataccaatga gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg
     7621 ttctcaatga cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg
     7681 acgatgcaga aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg
     7741 cagatggaca agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc
     7801 tgtacggaaa aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa
     7861 gatggaatga aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac
     7921 aagcggggag acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt
     7981 tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt
     8041 tatttcgttc attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt
     8101 tgtgcgatgc tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa
     8161 tctcattaaa aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa
     8221 aaaaacatat ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt
     8281 ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt
     8341 tctctgaaat ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat
     8401 aaagtgtaac aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg
     8461 agtacagtat tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct
     8521 gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc
     8581 gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat
     8641 tatgtatttg attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt
     8701 cataagcttt taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc
     8761 ggatattcca accaacatga aagtaaacat gactatttta cagtggggca cacatctaca
     8821 tctatcagct tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat
     8881 gtggtagcat ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac
     8941 aatggtggtg ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt
     9001 gatgagaatc atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa
     9061 agcagcaata acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac
     9121 gatctggaaa cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta
     9181 cttcacgtcc atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa
     9241 tatcattggc atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga
     9301 gaaacattta atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct
     9361 cctattgccg cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa
     9421 aaacatccag atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct
     9481 tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa
     9541 cgggcatatg atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta
     9601 gtcggtggcg gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct
     9661 tacttatcaa gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc
     9721 tctatgctag attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca
     9781 catggtgtag aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca
     9841 gtaaataatg gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa
     9901 gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg
     9961 atcacagaaa atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc
    10021 acattagaag atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt
    10081 gattcttcaa tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct
    10141 gtagggcagc tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg
    10201 cgtgtcttga atattcaata attcattatg ctcaccatcc agataattgt ttatactagt
    10261 atctggatgg ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag
    10321 aagaatatgt caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt
    10381 tcttttcatt tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa
    10441 agataaagac tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac
    10501 caaaagttgg tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca
    10561 aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag
    10621 aaggagaagg ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac
    10681 atgcttataa agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag
    10741 aatttgaagt aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt
    10801 ctcctgaata gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg
    10861 gaggagatat tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag
    10921 ctttggaaaa cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa
    10981 aagaaggcgg agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc
    11041 gaatcattga tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt
    11101 ttattactgc gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg
    11161 cactggatgc aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa
    11221 tgattcacga acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc
    11281 aagcgcaaga agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt
    11341 cacgaaagac gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata
    11401 tcccagtaat tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat
    11461 taggtgcttt ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa
    11521 gatatgtcga agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg
    11581 acaaaaaaga aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt
    11641 acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac
    11701 catgtttgaa cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg
    11761 ttcagtcatc cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa
    11821 tatcgcaccc ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc
    11881 gatccagaga aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga
    11941 agctgttatc catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa
    12001 tctgcctaat gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat
    12061 catttcagta ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca
    12121 tgtcgagata cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa
    12181 atatcattta aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt
    12241 gaatgctgta agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat
    12301 tgcacgtccc gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac
    12361 agattcaaaa gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta
    12421 cttagaagtt ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa
    12481 ggaaatagaa atgccagatc aaacgattag cggattgttt aattacttta acaaataaat
    12541 atttattccg aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa
    12601 gaatcctggt gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt
    12661 ttctaaaaaa tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa
    12721 tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc ttgttaggtg
    12781 aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa
    12841 cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata
    12901 gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga aaaggtttct
    12961 gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg ctcctcacct
    13021 gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt ggtggatttt
    13081 gggtccttat ttgactttgt ccatgatcgg tcttttagaa gtatacagtg ccagttctta
    13141 tcgtttattg caagccgatg aaaatacaaa aagtctatta ttgcgtcaac taattttcat
    13201 atttttgagt tggagcgtga tcttcttagc tcgttcagtc aaactacact atttacttca
    13261 ccctaaaata gcaggatacg gtttagcctt atcgattttc tttttagtat tagtaagaat
    13321 agggatattc ggtgtcactg tcaacggcgc acaacgttgg atctctctgt ttggcattca
    13381 attccagcct tctgaactgg caaatctttt tttgattttt tatttaagct ggttttttcg
    13441 tgacggaaat agtagcccaa aagatctaaa aaaaccattc ctgattacag taggtataac
    13501 ttttctgatt ttatttcagc caaagattgc tggagcattg atgatccttt cgattgcgtg
    13561 ggtcatattt tgggcagcgg cggttccatt taaaaaaggg atctatctaa tcgttacttt
    13621 ttctgcattg ctgattggag cagcaggcgg ggtattatat ttaggaaata aaggttggct
    13681 tccacagatg tttaatcatg cttatgaaag gatcgcgact ttaagagatt cttttatcga
    13741 tagtcatggt gcgggctatc aaatgaccca ttcgttttat gcactatata atggtggcat
    13801 atttgggcgg ggattaggaa atagtatcac aaaaaaaggg tatcttccag agacagaaac
    13861 agattttatt ttttcgatta ttacagaaga gcttgggcta atcggtgcat tgtgtgtgct
    13921 tttcttattg ttctcattat gcatgagaat cttttgttta agcagtcgct gtaaaaatca
    13981 gcaagctgga ttgtttcttt taggattcgg aactttgcta ttcgttcaga cgataatgaa
    14041 cgtgggaagt atcgcggggc tgatgccaat gactggtgtg ccattgccat ttgttagcta
    14101 cggaggaaca agttatctga tcctttcttt aggaatcgga atcacgttga atatttcttc
    14161 aaagatacag gccgaagagt tggcgctcta tcgacctgaa aaacaataaa gagaaactga
    14221 gaaactctca gtttgaatta tcataaagta caatctttta tttttcaagc gagaggttct
    14281 tttagaaaga ggaattcgca atgaaagtat ctcaaaaaat catattatct attttgttac
    14341 tgcttacatt agtagttggc tatttttcga ttgagtttat ccatggattt tcgtctgcaa
    14401 aacagagttc aactgtgaaa aaggtagagc cgaaaagtat ccctaccaca ctaaatgtgg
    14461 ctttgattgg ttcagatgct cgttcgaaag acgaaaacgg tcgctcggat tcacttctgg
    14521 ttgcacaata caaccagaaa acacaacaag caaaactgat ctctatcatg agagattcat
    14581 atgtcgatat tccaggttac ggaatgaata agataaatgc agcttactct tatgggggag
    14641 ttgatttatt aaatcaaaca ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa
    14701 gtatcacatt tcaagatttt atcgattgcg ttaatgaact gtttcctggt ggagtcaaga
    14761 tcgatgcaga aaaatctcta gatttagatg gcgtatatat aaacaaagga gagcaagtga
    14821 tggatggtaa tactttactc cagtatgctc gatttcggga agacaaagaa ggagacttcg
    14881 ggaggatcag aaggcagcag caagtgatca aagctatctc tcagcagctg aaagacgtaa
    14941 cttcaatatt caagctaccc aaagcggttg ggaaattact tggtagtatc caaacgaacc
    15001 ttccagaaag tgtgcttctg gattgtggta tggatttcct caaagacata tgttagaata
    15061 aacaggtata aatagtgaaa taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa
    15121 gaagtgacaa gcacggcaaa aatcgaacag gcgcttatat tgccggcgca gtgatcttaa
    15181 tagcagctgc gggtggcggt tatttttact accagcacta tcaagaaacc caagcagtag
    15241 aagctggaga aaagacagtt gagcaatttg tccaagcttt gaacaaggga gattataaca
    15301 aagctgcaga aatgacatca aaaaaggcgg caaataaaag tgcattatct gaaaaagaga
    15361 tcttagataa ataccagaat atatacggtg ctgctgatgt caaaggactt cagatatcga
    15421 atctaaaagt agataaaaaa gacgattcta cttacagttt ttcatataaa gcaaagatga
    15481 atacctcatt aggtgaattg aaagatcttt cttataaagg aacattagac agaaatgatg
    15541 gtcaaaccac gatcaactgg caacccaact tggtttttcc agaaatggaa ggaaatgaca
    15601 aagtaagcct gaccacacaa gaagcagcaa gagggaatat tatagatcga aatggtgaac
    15661 cattagcaac aacaggcaaa ctaaaacaat taggagtcgt tccaagtaaa cttggagatg
    15721 gaggcgaaaa aacagccaat atcaaagcga ttgcttcctc cttcgactta acagaagatg
    15781 ctatcaatca ggcgatttca caaagctggg tacaacccga ttactttgtc ccattgaaaa
    15841 tcattgatgg agcaacgcca gaacttccag ctggagctac cattcaagaa gtagacggca
    15901 gatattatcc tttgggtgaa gcagctgctc agctgattgg ttacgtggga gatatcacag
    15961 cagaagatat tgataagaat ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt
    16021 tggagatggc ttttgataag gatcttcgtg ggacaacagg tgggaaatta agcattacag
    16081 atgcagacgg tgtagagaaa aaagttctga tcgaacatga agttcaaaat ggcaaagata
    16141 tcaaattgac aatcgatgcg aaggcacaaa aaacagcttt tgacagtcta ggaggaaaag
    16201 ctggatcaac tgttgcgaca acgccaaaaa ccggtgatct tcttgcgctt gctagctctc
    16261 caagctatga tccaaacaaa atgacaaacg ggatctcaca agaagattac aaagcttatg
    16321 aagaaaatcc tgaacatcca ttcatcagcc gatttgcgac aggttatgct cctggatcta
    16381 cgtttaaaat gattacagca gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag
    16441 tgttgacgat caacgggctt aaatggcaaa aagacagttc ttggggatct tatcaagtaa
    16501 cgcgtgtcag tagtgatgta tcacaagtag acttaaaaac tgctttgatc tattccgata
    16561 atatatatgc ggcacaagaa acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag
    16621 ataaattcat ttttggtgaa gaccttgatt tgccaattag tatgaatcca gcacaaattt
    16681 ctaatgaaga tagctttaac tcagatatct tgctagctga tactggatat ggacagggcg
    16741 aacttctaat tactcctatc cagcaagcag caatgtattc tgtttttacc aacaatggca
    16801 cacttgtcta tcctaaattg attgcagata aagagacaaa agataagaag aatgtcatca
    16861 gcgaaacagc agtacaaacg atcgtgccag atctgagaga agttgtgcaa gatgtaaatg
    16921 gtacagcaca ttctctttct gctttaggga ttccgttggc agcgaaaact ggtacagcgg
    16981 aaatcaaaga aaaacaggat gtaaaggggc aagagaacag tttcttgttt gctttcaatc
    17041 ttgataacca aggttatatg atggtcagca tgttggaaaa taaagaagat gatgattcag
    17101 caaccaaacg agcatcggaa ctattgcaat acctcaacca aaattatcaa taaaaaattg
    17161 ctttttaagt acagtaaaag gactgtggcg ttatcgtcac agtccttttt tcaaataaac
    17221 ggttttcacg atgtctcttt cacaacttct ttttgattta ctcacacttc ataaactttt
    17281 tttgtaaaat atttgagaac aagtggagaa ataagtgtac ttaataaaat cacgataact
    17341 aaaggtgaat agtactgtgg ttcgatcaat tgactttgct gtccaatttg taaaatgatc
    17401 aaagccattt ctccacgcga aatcatacca gcaccgacca tcaatgaact atgttgagaa
    17461 aaaccggcaa tctgagcacc aaaataacct ccaagcaatt tggtcaaaat cgctacaagc
    17521 gtcaatatag aaataaagag tagttgttca ctaaagtcag aaaaatcaac ttctaatcct
    17581 acactgacaa aaaatactgg tataaatact gcatatccta aagcctctac attgtggtat
    17641 acttcttctt taacctttgt ctgactgaca gctatcccag caaaaaaagc accaatgaca
    17701 gagctaagcc caacaagatc cgctacgtaa gacattccta aacaaataac tagcgatacg
    17761 atgataatag aagaatttgc gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc
    17821 catttgacta ataagaaaat aaaaacaaaa taaattagct gttcaatcaa aacaagcggt
    17881 aaagaagcat tgctagaagc ttcccccgtt agaaaagata ggctgaaact tagaactaaa
    17941 acgactaaaa tgtcatctac cacagaagca cccaaaattg ttgatccttc ttttgtattc
    18001 actacgttta gttcttttaa tacttctact gaaatactga cagaagttgc cgcaagaata
    18061 atcccaaaga aaatcgcttc atttcccgaa acttggaatg ctttgcctgt caaccagcca
    18121 aaaacaaccg ggaagatgat cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt
    18181 ttcaaaagag ataaatcact ttccagacca gctaaaaaca ttaataagat cacaccaatc
    18241 tccgaaaagt catggactag aatatcaggt ttgacccaat tcaatccagc ttggcctagc
    18301 aaaacaccaa caagtaattg tccaatcact gctgggatac ctaatctccg cgagatatgt
    18361 gagccaatcg ttgttgaaat caatatcagg cataaaatac caataaattc cattctcatc
    18421 attcctttgt ataaaatata gcccgaactt agcataacat acattattga aaaaatagga
    18481 aaatatactc acaaaaaaac caacaatcca aaagattttt ttggattgtt ggtttttata
    18541 cggtctattt taataactaa aaatgattaa cgtatagtca tttttcttat tctgatgaaa
    18601 ttgataatct cctggatgcc catgaaaatc aaaataaagc cgaatatttg gaatagaagc
    18661 atcaaacttt tgaatggatt gaacaataag acgaatccgc caatcaacaa cagaatacta
    18721 ctataaacac tccaactttt agcttttgta ttcatgctga agaacaactg gaacaagctg
    18781 ttgatgataa cagctaatcc aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa
    18841 gcatagacga tcagagctaa gatcaggaaa aaaagaccac tgaacagacc gatactccaa
    18901 gaacctgttt tccttttaaa acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca
    18961 agataggcgg tgatcagata accgataaaa tgaaaaacag cagaagggtt tagcacaatc
    19021 gcaatcccag aaataatata aacagcagct cttaataagg cgtatttttg aatattttgt
    19081 ttaatcgtct caaacatata agaaaacctc ctttgactta ttttaacgtg aactagtcat
    19141 tttaccaaat taaatgttac aaaatgagcg aaggagaaga atatctctgg aaaaatcaac
    19201 ttctgaggat cttttccatt tttttgcctt ttgctagctc atcgatcatt ttatctaaat
    19261 aacgaatttc tttcatcaag ttatcctcga tgtcttctac tcgtacacca caaacaacac
    19321 ctgtaatcaa ggaacgtgaa ggattgggga caggggcttc cgcaaaaaat tgttcgaaag
    19381 tcacttgttt ttgtagttga tattctagtt ctgtttggga ataacccgtc agccaacaaa
    19441 tgatttcatc tacttcctct tttatacgat cttttctctc cgcttttgct acatacatag
    19501 gataaacgct tgcaaaactt gtcgtaaaaa tacgattttg cgccataatc agtcaccttt
    19561 ctttttttca aattatttac tacttatagt atataagaaa aagagaagaa aaagcgatta
    19621 ttcaaaaaaa caaaaaggat tgtgacaaaa ttcttgtcac catccttttt tccgctctct
    19681 gactttttac aatcttcata gctcaagtgc gcatcagata atcgaatgct cctaaagctg
    19741 cagtagctcc tgaacccatt gaaacaatga tttgtttgta tggactatct gtacaatcac
    19801 cagctgcgaa aacgccgggg atattcgttg cgccattttt ctcggtcact atttcatttc
    19861 ttggattcat ttcgatcgtt ccatctagcc aatccgtatt agggagtaag ccgactagaa
    19921 tgaatactcc tgaaacagct agtgtatgca tctccatcgt acggcgatcg atataggaga
    19981 gagattctac ctgattcgta ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga
    20041 cattcgcaag agagactagt ttatcctgta agatttgatc tgcttttaat tctggtaaga
    20101 attctagaac atatacatgt tttgctaacc cagccaaatc gattgctgct tcaacaccag
    20161 aattaccacc accgataaca gcaatctctt ttcctttaaa taaagggccg tcacagtgtg
    20221 ggcaataagc aattccttta tttttaaact ctttttcgcc tggtacattg atagaacgcc
    20281 agtgcgcacc tgtcgcgatg atggcggttt ttgctttaag tgatataccg ttagctaagc
    20341 caatattaat aaaatgttgc tctttattta aggaaacggc ctgttgattc atcataatgt
    20401 cgatagggta actacgcaca tgactttcta cttgctgcat caattttgga ccttctgtgt
    20461 aagaggtacc aatcatattt tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat
    20521 cagcgacaag tcctgtacgg attcctttac gtgctgcata gatagctgca ctactagctg
    20581 ctggtccacc gccaatcact aatacatcat aaggttcttt ttcaaacaac tcttcttcaa
    20641 tcaaagggcc agtgattttt tcaacaattt gttcgatcgt catgcgtccg ctactaaatt
    20701 cttctccatt taaaaacaca gttggcactg ccatgatttt tttatcatcg atttcttttt
    20761 ggaacatccc accttcaatc atcgtatgag taatatttgg atttaataca gctaatatat
    20821 tcaatgcctg tacaacgtcg gggcaattat gacaagtcaa gctaacataa ctgtcaaaat
    20881 gcaatgtttg tgttattttt ttgattttaa gactcaaaga ttcactgatt ttcggtgccc
    20941 gtccactaat ttgtaacata gccagtaaaa aagaggaaaa ttcatgaccg agggggatac
    21001 cagcaaaaac gattccactt tctttgccaa ctggattgat agaaaaacta ggtgtgcgca
    21061 tcgaatctct tttttcccaa gagattttag gagacatttc tgctatttcc ttcgtaaatt
    21121 cagctagttt ttgactattt tcactattat ccaaactaac agaaaaaaca ataggagatt
    21181 caatcagtgc taaatattgt tcgagatttt gtttagtttc tttgtctaac atatccacca
    21241 cctaaatctt tccaacaaga tcaaaacttg gtttcaatgt ttcgccactt tctttccatt
    21301 ttgccggaca aacttcacct gggtgggtac gtacatattg agcagcgcga atcttatcaa
    21361 tcagtgcact agcgtcacga ccgattccat ctgcattgat ttccatagct tgaacgattc
    21421 cgtctggatc aataataaat gttcctcgct gagctaatcc ttgctcttca tccaaaacat
    21481 caaaaatacg agaaatctga tgagacggat caccgatcat cacgtattcg attttaccga
    21541 tagcatctga tgtatcatgc catgctttgt gagtaaagtg ggtatcggta gagcaagaaa
    21601 agacttcaac acctaaagat ttcaatgtac tgtattgttc ttgtaagtct tctaattcag
    21661 taggacaaac aaaagtaaaa tcagctggat agaagcaaag aatactccag ttccccataa
    21721 gatctttttc actgacttct aaaaattctc cttgatgata agcatgtgca ttaaattctt
    21781 cgattttttt cccaatcaat gacatagtaa aacctccaat aataattatt tatttataat
    21841 tattgtaatt tagttggagt tgcaaagtca atggatattt tgttatttaa taaagattac
    21901 tcaaaaacga aggttaatta taaaaataga agaggtcgca agaaaatgct actgctggaa
    21961 atactcggga taacgttgag taacagtttc ctgttctttc agcatcatgt gtaagtgcaa
    22021 tcccgtttga aatcgagctt cttctggttt tttatcaact aaagccttga gtaaatcata
    22081 atgttgttcc atgatcgttt cccatggaag cccctgaatg gtcactcgta accagcggaa
    22141 acgttctaaa tgggtattat ggtcatctaa ccagttccaa acttcttttc ttcctgcaat
    22201 ttcgaaacaa gcttcatgaa ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat
    22261 tttgactgct ttttcttgtt cttctaaaat cgtttctagc actattcgtg tttgtgtgtc
    22321 caatttggaa caacattcaa tcatgatttg ctcttccaac tgttctcgga caaagcgagc
    22381 attttctgca gaaacaagat taattttgga aacatacgta ccactttgag gtatcgtata
    22441 cacgagttct tgctgacgta gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc
    22501 aagatgttcc tccagtgcct tttcagatat tttccttcca ggttctaagt cagcatagat
    22561 aatttgttta cgaatcgctt gataagcttg tgattgcaag ctgcttttca tctttattcc
    22621 tccaaaatca tttctatgaa cgtagtataa cagaagaggt ctgaaaaaca atctggtatg
    22681 tcacattaac tagtaaattt gtataaatag taaaattttg gttgacatag cgatttttat
    22741 tgcataaaat tcacttgaat acaaaaacta atatgttagt ttaggagggg aaaaatgatt
    22801 gatttaaaca actggaggaa ctttatccct gaattatctc aaaaagaagt agaaatacct
    22861 cattttgacc gtgcatcatt aagagaaaat ggacaaaaaa atcctgtctg gatccatttc
    22921 ggtggcggaa atctttatcg aggatttcat gcagaaatcg ctcaacgttt agcggatcta
    22981 ggcgaattgt ccactggtgt cattgtttgt gaaacattcg atgaagaaat aattgaaagg
    23041 gcttaccaag attttgatga tttgattctt gaagtggtca tgccaagcag tggagagttc
    23101 gtcaaacgta tattagctgc gacagctgac agtctgtatt gtcatccaga ttgttctgaa
    23161 cactttgata agatgattcg acactttgaa aatcctagtt tacaattagt tacttttgct
    23221 attactgaaa aaggttatcg attgaaagat acaaataata aatatatagg accgattgca
    23281 gtcgatataa aaaatggtcc aaagaagcca tcacatacaa tgagtattct tgctgtgcta
    23341 ctttataaac gttttttatc tggagcttgg ccgattgcac ttgtctctac agataatttt
    23401 tcacaaaacg gtagtcaatt acgtacttct ttgctaacga tcgcaaaaga atggcaaaag
    23461 gaaggttatg tacaaaatac atttatccaa tacttatcga atgaagaaat tgttgcgttt
    23521 ccatggacga tgattgatcg tatcaccccg aatccttcct tgtcagttag caaaatgttg
    23581 gcaaaagagg gcttttcaaa tatgtcgatt ttccaaaccg caaaaggaaa taatattgct
    23641 ccatttgtca atacagagga catccattat ttaatcatgg aagatcattt tcccaatgga
    23701 cgtccgaaat tcgaaaaagc tggggtattc ttgactgatc gtgcaacggt agataaagca
    23761 gatacgatga aggtgacgac ttgccttaat cctttgcata cgtctttagc tattatgggt
    23821 tgtttactgg ggtataggag catagctgag gagatgaaag atccagattt atcgctgcta
    23881 gtttctgaaa tcgggtacaa tgaaggcctc ccagttgtgg agtctccagg tatcattgat
    23941 ccaaagatct ttctggaaga agtaattact gtgcgctttt ctaatccaaa tattcctaat
    24001 atgcctcagc gaatcgccac ggatacctct caaaaggtgg cgattcgtta tggtgagacg
    24061 attaaaaaat atatggaaat agaagttaaa gacccgcaga cacttgtatt cataccattg
    24121 actctcgctg cttggcttcg ataccttatt ggcattgatg atcaaggaaa accattcatc
    24181 ccgagtccag acccactatt gacagacctt cagcataagc tttccgatat tcaactgggt
    24241 acgcaagata catcacatat tcatgagaaa atggctccta ttttgtcaaa taaagaaatt
    24301 tttggtgtag atctttacca agcagagctt ggcagaaaaa tagaagaaat gttcatcaag
    24361 atggtagcag gaccaaatgc cgtacgaaaa acattacagg attacttaca agaacatgga
    24421 ggaaatttat catggaaatg acttttagat ggtacggatc acaggaagaa aagattacct
    24481 tagatgaaat cagacagatt cctgcggtca aagggattgt aggcacattg atggatatac
    24541 cagtaggaga ggtatggcct agggagagga tcaaagaact aaaagaagaa attgagtctc
    24601 gtgggttaac actaaaagta attgaaagcg tcaatgtgca tgatgccatt aaaattggat
    24661 taccttcccg tgagcagtat atcgaaaact ataagcagac aatccgaaac ttggcagaat
    24721 atggaataga agtcatctgc tataatttta tgcctgtatt tgactgggtg aaatctgacc
    24781 ttgactaccg cttagaagac ggctcatcca cgttagcttt tatatctgat gatattccag
    24841 atgatccaag agaaatcgtc gcgcgcatcg aacattccag caatgaattt ggattgccgg
    24901 gatgggaacc tgagcgctta gctcctatca aatccttgct tgaagcctat gcatcagtag
    24961 acgaagaaaa acttcgagaa aattttgcct attttctcca atcaatcatc ccaacatgtg
    25021 aagaggtagg ggtcaaaatg gctgtacatc cagatgatcc gccatactcg atttttggat
    25081 tagctcgaat cgtaaagaat cgagaagatt tagattggtt gtgtcgcata gtagacagcc
    25141 cttataacgg aatcactctt tgtacaggga gtattgcaga agatccagaa aatgatgtat
    25201 atgggatttt agcagaattt tgccaaagag atcgaatccc atttgcacat gttcgaaata
    25261 ttaaattttt agctaatcga gatttctatg aggcaccaca taaatccgaa tacggttcat
    25321 tagacatgta ccgcatacta aaaacaatgt atgacaacgg atttaacgga tacatccgtc
    25381 ctgatcatgg aagaatgatc tggaatgaga caggaagacc aggatacggc ttatatgatc
    25441 gagcattagg tgctgtttat ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa
    25501 gtaaagataa ttagtgaaaa aaactgtaat tttattatgt aaataagaag gatgttcaaa
    25561 ttcttgatac ataaatccgg aggttgttaa taagcattcg gatttatgta tcttatttgg
    25621 tctaggaagt aacttgattt tcttttttcg attgatccat gagtgcttct tcttttaatt
    25681 caaagatata ttctgagatc ctcataatca cttttttcca gtcttctggt gtgatgattc
    25741 cttttaaaat atagattttt ttgatatgag aaggcaattg tttcaaaggg cagtcgctga
    25801 taacgatgtt gcagttctcc ataaaatcat tttgtatttt gatcgattca tttgaaaaaa
    25861 tttgttctat cttaacgatc agatctttac tgatataaaa atcacgcgta tagttcacac
    25921 caatcacaat tcgcggctgt tttttgtagc gatcatacaa atgtactagg tcttcaacca
    25981 tccattgcac attttccttt tctaaaataa ctggtaacaa gtgttgattt tgttctctga
    26041 aatccatata aaattgttca atggcggttt ggacttcttt caatacgcta tgttcttgat
    26101 aaagcatgaa agtaggaagg ggcgtatttg gttgattgaa gttaagaaat ccattataaa
    26161 gtttatttcg tgcgaatgcc aatgagtaaa gaatcttttc tttttcaggt ataaacaaat
    26221 caaatacagt acaaagggac tccaccattt ctcttgaata aagaacatga ggaacgtttg
    26281 ctttttttaa aagtttgtat tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag
    26341 aaagtcgagc taaaatattc actaagatgc gttgttcgcg acaaattggc agattaggtc
    26401 gcaaaatatc tacagagaga tcaataaaaa agtcaatata ttcatactct gtgaattctt
    26461 tttgtacgtc tttcacagaa gttgcaggaa aattctcttt acatattttt aatagaaaat
    26521 ataatttatc tatcataccg ctatttagtc ggtcaagtac ttctttttta aaaaaagtat
    26581 tcatcataaa agccactact tgcggatctt catcataaaa ttctgtagca gtacttgaaa
    26641 agatgttcca ataaatatct aatgctgcat attgaatttg tacctctttt ccagaaaaag
    26701 actttctgcc attattagaa aaggagatag acaaaccgta ctatgctaaa aatgcattaa
    26761 tctttttcat tttcgtatac aaataagagg cactatagtt tgtttgctga ctgaaactga
    26821 taatcgaatt tgtctgacag ctgaacaaag aagtcaagat ctgataactg actgattcgt
    26881 tgcagtatct tccaaataga cgataatagc agtaagccgg atcaattcct tcaaaattca
    26941 actttaaaaa aggtttttgc ttatcaatat gcagttctgt tagattgaga cgctcaaagt
    27001 cttgttctaa ttctttgatg tatagtaaaa aatttgattc agacatatgg aaatgatctg
    27061 ctaaatattg tgtagtaata aagaaacgat gttcagatag gaaaagaaaa atatctaatt
    27121 ttgtattgct ttttttcttc aagataaacg ttttaatcgc ttcttgagtt tgcatgaaag
    27181 aggctccttt ttacttaaat tgttaaaact tgaaaatatt actgtttttt tcataaattt
    27241 agacaaaaaa acagtaaaca tataggtaga atctctacta tcttatttct tgcatacaaa
    27301 aaaatcaagg agtgagagag ttatgagata ttcaaccatc ttaatatcaa catttgcagc
    27361 cttaagctta tcgcttacta taaatcagac agtccacaca gaggagacag aaacagccaa
    27421 atggacagaa accaagtggg ccaacgtcaa tcgaattacc tttcaggatg aattttcatc
    27481 cttcaattca ggagtcgcaa aattaagaaa aggacaaaca gcgaccatca aagcaaaaat
    27541 tgactattcg ggagacttag cacctatact acaatcagct gttacatttg agaatgttga
    27601 tcccactttg tcgattggct atgacgagaa cactttgact atccaacaca agcaagcgat
    27661 atttaccttc accgttacat taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa
    27721 ggtatcagat ggactaccga acgatcatca tttaacgccg tatagtcagc ggcagacaat
    27781 tgagcaagat tcagccatcg actcaggagg agatcttgtt gaagaaccaa cagataaacc
    27841 agaaaatgaa aataagccag aagtaccgcc tacagaaaat ccagacggag aacagaaacc
    27901 ggaaatagaa cctggagaag aacccgatac agaaactcaa ccagagccag acaataaatc
    27961 aaaacctgaa ataacccctg gagaaaaacc agatgtaact ccagaaccag atacggattc
    28021 tggaaaccaa acagtaccgg aaacaaatcc agacacagat aatgaaactg aaaatccaga
    28081 aaaaccagag gtagatccag aagaaaaacc agatgtgact ccagaaccag atacggacgc
    28141 aagagaccaa ggaataccag aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa
    28201 agagagtaaa aaatcaaatc tagcaattct aaaaataaat gaagagcagc tgaataaaaa
    28261 atcacgaata tttgatagcg cccaatcagc tgaaacctta aaaagtagca aggatacaac
    28321 ctttgctgtc ctgaaacgaa aaataaacag ctaccaaaat ccggagagtc acaaaacaaa
    28381 gttatactgt ggagcggaat cattctatta agtatagcaa caatgctttc tgcaaaaaga
    28441 tttaaacaaa acagatctct ctaacatttg aataatgtct gtgaacgcat ttttcaaata
    28501 aaaagaaata tccagaaaat gaccccaaaa taccagattg aaatctgata tttggggtcg
    28561 ttcatacttt atctttcttc atatttattt taagggatat ctgtttcttt tggaagatgg
    28621 ttcgggatct ttttcacttt caatacatct aaaaagaagg taaagatcgg aatgccgaca
    28681 atcaagcccc aaacgccaaa gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa
    28741 atcggcagtt ctgttttgct ggacatcaac ttcggattca gcacataaga ctcaattaga
    28801 tggacaatca aaatcgtaat caggatataa acaacatctc ggatgcctcc ttgggaataa
    28861 gctatgaaag atagaggaat acaagaaatg ataacacctg ctactgggat cagacttaaa
    28921 atgaaaatca tgatcgctaa actcagcagc tgatgaaagc caaatacagc caaggcgatt
    28981 gtagtgagca acgtatttaa aagagcgaca acaaattgag cttctaatac caaaccaaat
    29041 gtattgacaa acttatcggc aaaatagtaa atatcttgaa agaaccaaga aaattcactt
    29101 tttaaaaaca atcttgagaa tacaattgtt tttttctttt caatcataaa aaagaaactt
    29161 aagatgaatg acatagcaaa cgccactgcc atttttccta tatcctgtaa atagccaagc
    29221 gcaatcgaag cgccattttg aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg
    29281 atatattgta aaacaggatc tgcatctttt ggctgatgct cgtaaaaatt aataacggac
    29341 tggatcatgt caaatgtttg atgaataagc atcgggatat actttgtgaa tgccagatag
    29401 ataaggtaaa cgatcagtgt ataaatcatg atagctaaaa cacctgtagg gattcggaca
    29461 aatcgctgaa tgatttgaac aagatgaatc atcaaataag taaagataaa tgtgagcaaa
    29521 atggttgtaa tcatacttct aactaagaaa agaacaaaaa cgacaaataa taaaacagtg
    29581 agacggcgta ttttttcatt cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc
    29641 aaataacaaa aaataaacac gttattcatt atatgatgga atatgaaaat aagaaagagc
    29701 aaaaagaaaa accgctaaat gacgttatct tatgagttgg ttttcctcat aaaatcgttc
    29761 atctagcagt ttcagccctt tttctggtct tgatgaaaat tatctagcat ttttcgggtc
    29821 agatgttgca gtgtatccaa ttcttcctgt tcaagtggaa gataagactt gatcttttgt
    29881 ggaatacctt ctgcttgttt ttgcaacgca gcaccttgag cagtcagcgt aacatttacg
    29941 attctttcat cacccgtttc acgctgacga ttaatataat ttttttctgc taactttttt
    30001 agtaagggag tcagcgtacc agaatccaga cacaaatcat ttcctagctc tttcactgaa
    30061 gtctttttct tttcccataa aactaacatc acaagatact gggtataggt aagattcagc
    30121 ggcttgagga ggggagtgta gtgtctaatg atttctttag aggcggcata taaaggaaaa
    30181 catacttgat taactaaata tagatcttcc atttttccac ctccgctgtt ttactatagt
    30241 gtatcatagc ggatttacac aaaaaagtct attcactaac tgctttcaga gtaacttcta
    30301 tatttccagc tgtagctttt gaatacggac acactttatg cgctgcctct aataattctt
    30361 gcgtttcttc cagagaaaga ccttcgatag atccttcaat ctctacacct aagacaaagc
    30421 tcgtttcact ttctgaataa agggaaactt gtgcactgac agtagaagcc ccagagatat
    30481 ttttttgctg tttgatcaat tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa
    30541 acaattgttc agggttggtt gcattttcga cgcgtttacc aggagctgca atattcaatt
    30601 cgaatgtacg atcgggatta tgcacttctc cagagcgtcc gcctgtgttg atcattttcg
    30661 tagaatacat ttttttcata aaattaccac ctttaattat attttataca attgaatttt
    30721 acacaatata attgtggaaa gcaaagaaat ggattggcat aagataaata agagaaagca
    30781 catgaaacag acgcgctttc tcgaatagca aacatataca aaaaatatca gagacaaaaa
    30841 ataatctcat cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaaat
    30901 tacttcacga tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga
    30961 gttagtcaca ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg
    31021 taatgcttta ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt
    31081 tggatgttca gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa
    31141 tctcgtacca tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc
    31201 aatcagttgt atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt
    31261 taatacaaaa tcgatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa
    31321 cagttactag attcttttat tataccagct aaagaaaacg cattcaaaaa aatagcggat
    31381 ttacttacca actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac
    31441 gctgctgaca atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga
    31501 aaattcatga tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc
    31561 ttttgcctct agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc
    31621 ttccaaataa tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat
    31681 ataagtcatg acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat
    31741 tccaaaaata aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga
    31801 agtattcaag tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga
    31861 agagttgttt ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac
    31921 agacaacaac cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa
    31981 aaccaagctt ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag
    32041 taccaacata acgatattca acactaaaat aactatagct cggtcaaagc ctaaagcagc
    32101 ttccagtaat attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa
    32161 catattgata gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat
    32221 aaattgtctc gtttctttca tttattcact tccttaattc attaataata ctcttatctt
    32281 attataactt acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta
    32341 aattattatg tttcattctt ctagaatata cttctacgcc aaaaactgtt aaaaaacttt
    32401 tactttagaa aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca
    32461 ggaagtagac taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata
    32521 aaaattcggt atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgcttc
    32581 tctatcactt atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc
    32641 tgcaatttat catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg
    32701 tattcggctt cgtactagga aaaatgatat caaaaaagta tatgtgctat gtggagatcc
    32761 atataccata acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag
    32821 taccaatgtt catgattatt gggaaattga agtgacgagc gaaacaagtc gcttacagta
    32881 tgcgttccat gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt
    32941 tccttatcga gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca
    33001 tcaaatcgat cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt
    33061 tcccgaaaga tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg
    33121 aagtaaagat ccagaccgag aagacttttt cgggggagat ttgcaaggtg tgatggatca
    33181 tttggattac ttgacggacc ttggcatcaa tggcatctat ttttgtccca tatttaaagc
    33241 cacatccaat cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga
    33301 taaaaaactt ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt
    33361 cgatgctgta ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa
    33421 cggagaaaaa tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta
    33481 tcagatgact gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac
    33541 tccattcatg ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat
    33601 cgcaacttat tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga
    33661 agtcgatcat catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat
    33721 ctatatcctt ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca
    33781 tgcagtgatg aattatgcat ttacagattc gattaaagat tattttgcaa aaaagaaaat
    33841 cacagcttca caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt
    33901 gaacgaagga acattcaact tattagattc tcatgatact gccaggatct tgaccctttg
    33961 ccaaggtaac aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc
    34021 tccttgtatc tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg
    34081 caaatgtatg atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga
    34141 acttgtttct ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct
    34201 aatagtaaat gatcgtgaag ataagctcta tttcacacgg aaactagagg gacagatcat
    34261 ctatgtttac tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat
    34321 tcttagtcaa aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt
    34381 tttgatcatg tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat
    34441 ttgtgcccat tataataagg gtgtaacagc acgttgaata caaaggagga aatgaagatg
    34501 aaaaaaagaa gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga
    34561 ttagcggctt gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg
    34621 ttaacagttt ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc
    34681 gaaaaagaaa acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa
    34741 tcattagcgt tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac
    34801 cggatcggtg cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca
    34861 gcttacgatg aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct
    34921 gcagtaatcg agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca
    34981 acgtttaaag atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca
    35041 ggaaaaaata caggattttt agctaaatgg actgactttt attattccta tggtttaatt
    35101 gcaggttacg gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg
    35161 aataatgcag gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg
    35221 ccaaagggaa tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc
    35281 aataaaactg cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat
    35341 atcaattatg gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt
    35401 ggcggaggaa aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa
    35461 tggcttgatt atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa
    35521 atccctgcaa accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca
    35581 gctgtgattg aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa
    35641 gtttggacag gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa
    35701 gaatccgcag atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta
    35761 aaatgataac cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag
    35821 agcgagatgg aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta
    35881 gcaagtttag agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa
    35941 aaatcacgct ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta
    36001 aaggtatcgt ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat
    36061 ttcatgcatt ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat
    36121 ttattttgat tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg
    36181 gtttgaatct tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca
    36241 atcggacgat gaaggaagtc atacataata taggtgatga aggttttcct tatctattga
    36301 cacttcccgc ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt
    36361 tcactgcatt tactaattat gacttcaatc atattccacc agccagttta attgattggg
    36421 taggggctaa aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg
    36481 cagtcttttc gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg
    36541 gtattctcac agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta
    36601 tcattttctt attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata
    36661 tattcaacga tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc
    36721 cttttgtaga tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa
    36781 ttatcttagt gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct
    36841 tacaatcgat ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc
    36901 agcgcttttc aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta
    36961 cacagtatac ggggaatttc aataatttct ccatgatcta tctgtttaac aatggcggtc
    37021 ccggaagcgt aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac
    37081 ttacgactgg aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg
    37141 tcatcgttat ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg
    37201 attaacaatg aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt
    37261 tacttacttt tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc
    37321 aagtactgcc ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg
    37381 gactttagga aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa
    37441 tacattgatc atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg
    37501 ctatacttat agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt
    37561 gattcagatg gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt
    37621 agaagcgctc gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat
    37681 gaatacttgg ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc
    37741 taaacttgat ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa
    37801 accgatgctc gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt
    37861 agctaagttc ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt
    37921 tatttctgat gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc
    37981 actaccgatt tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc
    38041 tggcggaaca aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc
    38101 cgcattacgt acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt
    38161 tttcttgata tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc
    38221 caatcagatc caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga
    38281 acaaggaacc caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca
    38341 acaaacgata gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca
    38401 atccgatcat ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga
    38461 taaatctggt atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt
    38521 acgtacgccc gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc
    38581 aacaatcatt ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct
    38641 cattttattc ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat
    38701 tcatacattc aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc
    38761 agcagcagtt gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac
    38821 gataactgtc ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt
    38881 cgaatgacaa gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt
    38941 agtttttgaa aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta
    39001 ttaacgtaat agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa
    39061 tcaattattt tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac
    39121 gaaacaatac ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat
    39181 cccaatgaaa gagattacaa gtaacgcatc tttggcacta ggagaagcct atatggatca
    39241 tcgaatcgag atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa
    39301 cagcttttta cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa
    39361 acaaaaaaac aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact
    39421 ttggctagat ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt
    39481 ggaacaagca caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg
    39541 cgaaacgttg cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga
    39601 gtataatgtc aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa
    39661 aagaataaaa gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga
    39721 actaaaaggc gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc
    39781 tgaaaatctt gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc
    39841 tttgatccat ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata
    39901 tattttccct ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa
    39961 cgatttgcaa ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca
    40021 ttggacaaag aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt
    40081 ctatcgaatg tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat
    40141 cgatgtgatt caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc
    40201 ataattgagg caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc
    40261 tccagaagtg aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt
    40321 ctttatattt tcataataaa agtttggttc tttaattttt aaacatgaca gaaaattttt
    40381 tcttcgttat acttgtaata gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa
    40441 aaactgttat ttatactagt ttatttgcag ctttgttttt agctgcttgt caatcaaata
    40501 ccaatcaagg cacatctggg aatacgtctt ccagtagcga acagcgctct tcacaggtat
    40561 cggactcctc aacaaaagca agcacatctt cgtctagcca gacaaaaagt tctagtgaag
    40621 caagtactca gtcagatatc aaagtgtctg cagctcgtgc gatcgaattg ttccaagata
    40681 aatacccaga tactgcaatt actagtttgg aattagatag tgattgggga tcttacttct
    40741 acaagataga aggtgtcgat gatcaaaaag aatacgaagc aagaattgat gctcaaaatg
    40801 aaaaaatgga gacagagaat ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga
    40861 aaaaagaaga tggtctggat gttgataaat tgatcacgat tgaggaagca gcaaaaatcg
    40921 ccgtcgacaa agtaggaagc ggtacggcaa cagattggga tttggataaa gatttaggta
    40981 ctacttattg ggaagtcaaa gtaaaagacg gcaatcaaag tacatcagtc aagatcaata
    41041 gccaaacagg cgaagtactg tcaactgaac aagatgatta agaatttctt cggaaaacaa
    41101 gagttgattt ttcttttagt catgatacac tagacaaaat aacttgtttg aaggagcgat
    41161 ttacttgagt ttagaaacga cctttgccaa tcatatcttt gccaatcctt tgatgaatgc
    41221 atctggtgtc cactgcatga ctacccagga attagatgaa ctggctcatt cagaagcagg
    41281 agcatttatc actaagagct gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta
    41341 ttttgatgtt cctttaggga gtatcaattc tatgggactt ccaaacttag gtttttctta
    41401 ttatctagag tatgctcttg cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt
    41461 tttttccatt gccgggatga gtgtccagga aaacttggaa atgcttgaga aaatagaaaa
    41521 aagtgggttt aatgggatca ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc
    41581 acaactagct tacgattttg aggccactta tgaaacactg aaagaagtat tcagcatctt
    41641 ttctaagccg ctaggaatca aattgcctcc ttattttgat tttgctcatt ttgatcagat
    41701 ggctgatatc ttgaatcagt ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa
    41761 tggtttgtat atcgatacag aacaagaagc agtagtcatc aaaccaaaag aagggttcgg
    41821 cggcattggc ggagagtata tcaagccgac tgctttggcg aatgttcgcg ctttttatac
    41881 acgtttgaaa ccagaaatcc aaatcattgg tacaggtggg attcggacag gacaagatgc
    41941 atttgaacat ctgctatgtg gcgcaagcat gttacagatt ggtaccgagc ttcataaaga
    42001 agggccggaa attttttcta gaatcataaa agaattaacg cagattatgt cagaaaaagg
    42061 atatacgtca attgacgaat ttaaaggcaa attacgcacc atttcatagc aaagagacga
    42121 aggtcatgat tgatcttcgt ctttttttat aaaaaacact cgtctgttca ttcctgtgca
    42181 agcgtactca ttgtatgtta gacgaacttg tgggaaaata acagaggcaa aaaggaggat
    42241 aatatgaatg atgcaacaga attagcaaaa gagcttaaag aaaaagaatt tagttttcaa
    42301 gaactagtta cggatataca gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt
    42361 gtcgcgcttg aaccattcga gagtaattcc cacgagctat tgattcccca gtcagaaaaa
    42421 gggccattgg ctggtatccc atttccttta aaaatgctcg gtcaagaaaa aaagggatgg
    42481 ctagctactt caggatctcg tttatttgaa acacaccgag cttctcatac atctaattat
    42541 gtgcggcaag cagaagcgat aggtctagtt ccttttggac aaaccaatgc gccagagttt
    42601 ggtttcaaaa atattacaga cccagtcatc tatggcccag caagaaaccc ttggaacctt
    42661 gaatgtacac cgggcggatc aagtggagga gctgctgctg cagtttcatc aggaatcgtt
    42721 cctttagctg gtgctagtga tggcggtggt tctattagaa tacccgcttc gttttgcgga
    42781 ttgattgggc tgaaaccttc gcgagggacg atgccagttg gtccatatgc ttggagagga
    42841 tggcaaggag ctgctattga tttcggcttg acagtttcta tgagagacac ggaggcttta
    42901 ttttatggga tgagaagtat ccattccggt gcgccatatc aagtatcgcc agttgagtgg
    42961 caaacacatc caagaaaaaa acggttgaag atagcttttt gtgttgattc accgatccac
    43021 tcacagatta gtgaagaggc agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa
    43081 gaagggcatg atttgacaga gattccttac ccactagatg gtcgtaaact gatcacgtct
    43141 tattatcaga tgaatggcgc agaaacagca gcaatgatcc gctcgattga aacaggcatc
    43201 cagcgagctg tcagaaaaga agaacttgaa cccatttctt ggactttgtt ccaatatggt
    43261 cagaagctct ctgccgctac ttacatcgat tctctgcatg tatgggatga agcagcaatt
    43321 gtcatggaaa acctacatca agaatacgat ctattccttt caccaacgac tgcatttacc
    43381 gcccctaaaa tcagcgaaga tttgcaaagt gatgcgataa gagcacagat ggaacacatc
    43441 agtgattgtt ctgaagtaga aggtttagac gttgtttctt ccatgtttga ggagagcttg
    43501 aagatcacac cttatacaca gttggctaat ctgactggac aaccagcaat cagcttgcct
    43561 actcatttga caaaagaaaa catgcctcta ggaatccaat ttatggctgc cagaggaaga
    43621 gaagatttgc tatttcaaat cggctatcta tttgaagcaa atcatcagtt ccatttacca
    43681 aatccatgga aatagcagaa acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc
    43741 taataactag tgcctttttt attttaagcg cttgcggtac agctgccgct aatcaggaag
    43801 ataacagctg gagtaatgta gaaaaaaaga aagaattagt agtagcgact tctggcacac
    43861 tgtttcccgc atcttattat aatgatcaaa atgaattagt tggttatgat gtagatttgg
    43921 caaaagaggt ggctaaacgg ttaaatgtca aaatctcctt taaagaatac aacgtggatg
    43981 gacaaatcac ttctgtttca aaaagagaat cagattttgc tgcgaacgat tttggtatca
    44041 caaaagaaag agaaaaaaaa ttctcccttt cagagacttt caaatattca tttgacagta
    44101 tgatcgtacg aaaaaaagat gattcaggaa tccactcact tgaagatctt aaagggaaaa
    44161 aagcagctgg cgaacccaat actagctata tgcgtttagc ggaaaaacac ggagctaaat
    44221 tagtcacata tgacaatgcc acgaatgatc agtatttaag cgatgtagca aatggtcgaa
    44281 cagatgttat tctaaatgat tattatctgc agaaaatggc cgtatctgct ttaccagata
    44341 tccctgtaaa aatactggag aacgtttatt tcaatgaaaa cgaaagtggt tttttattga
    44401 aagaaggaaa tgatgctctg aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag
    44461 atgggacgac aaagaaaata tctgaaaaat attttcaaac tgatgtttca gtcaaaccga
    44521 aagaaacgat ggctgagtcg gtgagtgagt gatgtacttt cctctaattg atttttccct
    44581 catgtggact tcacttcctt tcgtactgca aggcttggta tacacattag ggattggctt
    44641 cgtgtcgttt cttttaggaa atctggtagg gttgcttttg acagttttag gtcttttgga
    44701 ttggctgcct ctgaatgtat ttattcgttt ttatttatcg ttctttagag gcattcccgc
    44761 actagtcctg ctgtttctgt tatattttgg gttaccttat caattaagtg ccttaacggc
    44821 ttcagtcatc tgctttacga ttaccagcag tgcatttatt ggtgaaatct atcgaggatc
    44881 actagcaggc gtttcttcag gtcagtggga cgctggttta gcttctggtt tttctttctt
    44941 tggggtattg aaatatatcg ttttgccgca agcttttcga atctcagtac cggcactcag
    45001 taacgtagct atggatctag tgaaaggtac ttcgttagca gcaacaatca cagtgccaga
    45061 aatatttcaa aaagcaaaga tcataggcgg tcggacattc gattattttt ctctctatat
    45121 tttagttgca ctgatttatt gggtcatttg tatcgggatc gaaaaagtgc aacagtattt
    45181 agaaaaacgt tctatgcatc agtcaatctg aaaaatagaa atggtgctag cgtggctggc
    45241 accatttcta ttttttgttt cttgtatatt ctgcctagta ttttaaaacc atttcatgta
    45301 aaatgtaaat cgaaaatatg ttcggaggta gaaaacatga agaccccgct aaccatcgaa
    45361 atagaatcct cgttatacca atattgcatc gagcaaggcg gcttggttgt agaagaagtc
    45421 acgatgcctg atgaccaagg aattgtagat acgcttagct gcttgacaaa atttgatgga
    45481 agccgagaat ggcgatgcta tgagctgaaa gtgacaaaag cagattttcg ctctacagcc
    45541 aaactgtcat ttgttggaca ttataattat tttgttttac caagtaaact atatgaagaa
    45601 gtaaaaactg aaatccctac aggaataggt gttttagtgt atcgtccgta cacggttgaa
    45661 gaggagatgc ctgcctctgg tacatttctg attgcgaaaa aagcagcaaa gaaagagctt
    45721 gctgtaccgg aagaagcttt gacaaaccgc ttcatggctt ctttattcag ggaagtaaga
    45781 aaagcaaagc aaatggagcg aggcgtaaaa tattttccaa cagatcaatt gtacaaagaa
    45841 ttgaaaagac gttcagaaaa tcaagatcca ttttctaacg aacgatatta tgaacggttt
    45901 ttagaggaaa tagctagtca ggcaatcaat gagttgcaag aagaagttat cgcgcttcag
    45961 catgattatg aatatttgag gcaacaacag cagatccgtc ggttgcctac agagccattg
    46021 gagtgaaaaa tatgtactat ccttatttaa gaggaaaaca atttgaccta ttagcattaa
    46081 aagaagcact gtcgcgagga ttgctctcca ataaaatcca accagtgatc gaaccagtac
    46141 gtgattccgc tacgcttaaa aatgtgatcg aattatttca aaaaaagcac catcgaatcg
    46201 ctgtgatcca aaatccccaa gtaggacaat tcaaattatt cgatcagcat gtgcacagtt
    46261 ggacagtcaa agaaaatagc tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca
    46321 gtgaagtact ggattcacct ccagcgtttc ttgttttcga tggacagcac tacccaaaag
    46381 atacagaggt gtggaaacag ttggcaggag ttgattcgaa atttttgata ccagatacta
    46441 gtcgctttcg catctggctg cccgaaaaca aaattgtgat ccgtgactct tttcaaacga
    46501 gaaagcatgt tgaaagttat gcagataaaa cagatgactt ttttagtgat gactatcttt
    46561 ttttccacgc agacggatat attggatttt ctgatttcac gatcgaaggt agccgttatt
    46621 tcgataaagg gttccctagc cgagcgcttg ctatccatct aacgtatatt gatgcttacg
    46681 gcaatatacg cgtgaaacat tttgtctctg acagtaacga ctctgctaaa gaccaagcat
    46741 tgaagtttct tgaagcagga gataaaatga aaaaatggat catgcgtcac catcatcaac
    46801 tcttgattac ttcaggaatg attgaattat tggctcttta ccagcaacaa aaattccctg
    46861 gactgggagt gttgaaaaag tggtcactta tgcatcattt ggaacttatt agtcacctgc
    46921 tagatcatcc aaaagattgg cttaaatatt attcaaaagt tcaggaacta tatgaagtga
    46981 tccaatgatt agataatgta aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt
    47041 cggcaaacaa ggaaagaaga agtagaagaa gtggtagaaa tcattaatgc cgcagcggat
    47101 ttcattgctt cgcaaggctc tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg
    47161 attttgaacg atattgaaag aaaagaaagc tatgtattaa tagtggaaga tcaaattgct
    47221 ggaacagctg cattagtctc aagcgtggat cccgtttaca caaaaataga tggtagttgg
    47281 aaagaatcaa ctggtcctta tttatcgatt catcgtgtcg ctttagcaga aagtactcga
    47341 ggaaaaaaac tagcaaaacc gttcttagaa catttgttga cgattgcagc cagcaaaaat
    47401 atccaagata tccgtatcga tacttatcca gctaatgaac cgatggaaaa gaccatctat
    47461 tctgctggat tcacttatca agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa
    47521 gcatatcaaa taatcatcaa ttgactataa aatcatctga gcaaagataa agacattaac
    47581 actactgaaa aaaacaatca tatgatagcc aagaggttgt aacagaagtg gccagcttca
    47641 aaaaacaaga aagaatttcc gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt
    47701 tacgaaatag ctgctttctg aaactccctt catcgagagg tcgtgacata gcttttgtca
    47761 cgacctctcg gcttatccta cgagtattat tcgatacatt tatatcaata atcctttttt
    47821 tattttatct gcagcttctt ggaacgctcc atcgtttgac acgactaaaa gtgaggggat
    47881 agctttcaaa cgtttcaact gtgctaaatc ttctacataa accgccagtc tggctgaaat
    47941 ctgttcagat gaatctccac gttttttcag gcgcgattgc aatgtttctt tagagatcgt
    48001 aataaagata ggacggacta ccgtcccgaa atactcatat aaatgccaaa aaccatttgc
    48061 tgtcaaaacg gcgtaacaat ttcctactgt cagtttcttt ttaatttctg ctttactgga
    48121 accatattta tgttgagcat attggtccca ctctgcaaac tcatttccct tgatcatctg
    48181 attgaatgta ttgtcagaaa caaaataata atcctcttga tctttttccc caggtcttgg
    48241 tgatcgattc gtgtaagtaa tgatcttttg ttccggctga aaaatagctt ctgcgagtga
    48301 agtttttcca gaaccagaag gaccgataaa tacatagcaa taattcattg tcatttctcc
    48361 tttgatgatt gaacaagtga aaaagctttg gaaaatattt ccaaagcttt taaaagtaac
    48421 tttgcttttt tgtatattat accagcaagc cagtcacaat agcacttaaa aagctgacta
    48481 aagtcgctgt tagcaaaatt ttcaatccgt gttttgcgac aagatttcct ttttcttcat
    48541 ttaatccctt catcgcacca gaaatgatcc cgatagaaga gaagttggca aatgaaacga
    48601 ggaagacaga tataattgct gttgtacgct cagtaaaatg gaaattacca tttgctaatt
    48661 ctgtcatagc aacaaattca tttgtaacca gtttagtcgc catgatacta ccggcatcca
    48721 ctgcttcttt ccatgggatt ccgctgatga aggcaagagg ggcaaagatg taacctagga
    48781 tctcttggaa agtgatgccg aatatcccgt ggaagatagc attgatcatg gcaatcaaag
    48841 caacaaaacc gataagcata gcagcaaccg tgatcgccac atggaaacca tcaagaatgt
    48901 attctcccaa catttggaaa aatgtctgtt ctttgttttc ttcaatgatc agctcatctt
    48961 ctttttcttc taatacatag ggatttacaa ttgaagccag aatataaccg ccaaataagt
    49021 tcaaaacaag ggcagtgatc acatatttag aatcgatcag tgccatataa gatccgacga
    49081 tcgacataga aactgtagac atagccgata cactcatggt gaacaaacgt ttctcagata
    49141 ggaagggtaa ttcctttttt atggaaataa aaacttccga ttgtcctaaa atagcagaag
    49201 ctactccatt ataagattcc aatttgccca tcccgttgat tttacttaat cctagaccaa
    49261 cagctttcat aaataatgga agaatcttga tatagcgtag aataccgata atagcagaaa
    49321 taaatacaat tggcatcaat acggaaagaa agaaaggtgc acttccttta tttgctacac
    49381 cggcaaaaac aaagtcaaca ccttgcccag caaaagcaag aagatgatca aacatttttg
    49441 ccaatacaga aacaaccgct gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa
    49501 gctgtaaaac gaacatcaca attagtggtt tcaatttgat tgcttttttg ttactgctga
    49561 tcaaaaaagc aagccctaat gtgacaaata aaccaataat acctaataaa taactcaaat
    49621 aatcactcct attccaaatt ccgttttatt ttacctgttt tgcaaataaa atactaatct
    49681 ttagtgaaat tttcattact tttttagagt gaaacgcatt cattttccat tagaaaataa
    49741 agcgaacaaa ataaaaggtt taaaaagtgt aacgtcttag taaagtctat aaaaaaacga
    49801 aactatcgat taagctaata gtttcgtttt caacatttat aagtaaactt tttctattgg
    49861 taatatagat tttcggaagg gtagacaggt gcgcaggtaa tatcgattcc tagttttttc
    49921 aatgtttgtt catcattttt attgaccatc acagtagagt gggcttgtgc tccctcaagt
    49981 tcagctagtt tatcataagc aacttgagcg attggattcg ttactgcact gattgataaa
    50041 gcgatcaaga cttcattgat gcttagtgca tccaaagagg aatgaagtgt ctttgatttc
    50101 aatccttgta ttgtttcaag aactaatggt gacaataaag gaatgtcatc tgcgatatgg
    50161 gctaaatgtt ttactgcatt taagataact gccgctgacg catccatcaa tgaacttgta
    50221 cgcccggtaa tgatgacttc atctggtaat tcgattgcaa tcacagcaga aggatctttt
    50281 gtaggacttt gcgcttgaat cttatctgca tagtcatggg cagggactac cacgtttcgg
    50341 tcttcaggac gtaaaccgac ttcttccata atcagtttga ttcgattaac tgcttcttcg
    50401 tcgatcaatc cttttttgaa gtcgcattct gtttggaaat aacgacggat gatttcttgt
    50461 ttagaggctt cttggacaac ggtatcatcc acgatcccaa atcctacacg attcacaccc
    50521 atgtcagtag gagattgata aacagattct ttaccagtaa ttttttcaat aatacgtttg
    50581 attacaggaa atgtctcaac atcccgatta tagttcacag ctactttatt gtaggcatca
    50641 aagtggaagg aatcgatcat attgacatct tttagatcga ctgtggctgc ttcataagca
    50701 atgttcagcg gatgtttcaa cggaacattc catactggga aagtttcgaa cttggagtaa
    50761 ccagctgcat gtcctttttg gctttcatga tacagttggt tcaaggaagt agcgagcttt
    50821 ccacttcctg ggccaggagc tgtcactaca acgattggtt tagttgtttc aatatactca
    50881 ttttttccaa aaccatcttc tgagacgatt ttttctacgt tgactggata gtcttcgatt
    50941 tctgagtgtt tatagacctt gatgtttcgt ctttctagtt tattgataaa gactttcgtg
    51001 gcaggctgtc cactatagcg agtgataacg acactgttga ttaataagcc ataaccacgt
    51061 aattcatcga ttaaccgcaa gatatccata tcataagtaa tcccataatc accacggatc
    51121 ttattacgtt caatatctcc agcatagacg caaatcagta tttcagcttg atctttcaat
    51181 ttttgcagca atttgatttt cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc
    51241 ttgtcgtcaa ctagcttccc accaaattct aaatataatt tgtcatagtg gttcactcgt
    51301 tccaaaatat acgctgactg ctcttcaata tatttttctg aatcaaaacc aatttttttc
    51361 actaggttcc ctccttaagt actaaagcat tttttcattt ttttgaggat tcgtcaactg
    51421 gaagagcgaa attcctcaaa aaaacaaaga aaatggttta aaccaacttg tctagatagt
    51481 tattcattgg tatgatagtt tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa
    51541 gaaaaacgca tataaacact atatccgttc gatttttacc ggttatgaaa gaatgcttga
    51601 agatcccgaa ttggaacaat tgacgtttac ttatctaaac gaagaaacgc agctaactcg
    51661 tgatgagcat cagcgcattc atttcacaac aagagatctg cctagtaaat aaaaaataag
    51721 ataaaggtca tcggattcga ttctgtccta tcagatggaa agactagttg atcgaaaaaa
    51781 aggctgtacc aaattttggt gcagcctgtt ttatggagca ttgcccgctc ttttttataa
    51841 caatttttta cagaaaatgg tactattgaa gtattaataa gtgaaaaaca gatgagtaag
    51901 aggaggcaag tagtatgaaa gagccagata caaatgcaaa ttatgagcca gacgaactag
    51961 aggagacgaa tgcggatcag gatacaggac aagaatcgtc ttctttttac gatatcaaag
    52021 aaaaagcaag catgaatctt tcccaattat tagaagactt acaggctttt gaaaaagctg
    52081 tccgcgaaga agatatccct gagatctacc gtttatacaa tggccgattg aatgaagaat
    52141 taaaaagaag ctcaaatgaa aatcacgaga tcgatcaatt gctgatgcgt aagatccatg
    52201 atcgtttttt acaggaattt ccttttatgg aacaagtaga aaacatctcg ccaacgatga
    52261 gctattacaa aatcggcact tattaccatg atcggcccac ggtcggtatt gatgcaagtc
    52321 tgccagaaat ttttgttttg cctaagatcg atgaagagtg ggagaaatat tcccaaccgg
    52381 agtcaacgga ctatgacaaa aaaatcaatg agctagacgc aaaggtgatc actgctcaga
    52441 cagaaattga acgtttagat gaacaaataa aagaaatcaa tcgacaaatg actgatttga
    52501 acgacaataa aggattcttg aatcgtaaaa aaatcgagga agaaattcaa gaattagaga
    52561 agaagaagca agtactatct aatgaaaaat taggctggct tccttatatt gagacgcctg
    52621 aaacaatcca gcagcaaaaa gaagcattga agcaagaagc aagagcagac cagcttcgtg
    52681 cagccatcgt agaaaaagaa caaaggcaaa tcagacgcta tttcggtagc aaagagggat
    52741 tcggtcaagc aatccatgaa ttcttgatga attatcttgg caatgagaat cctaataatc
    52801 aatctaatga aggaggttcc gagtatgaat gaacacgatg aagcagaatg gacaaaaaat
    52861 gaagaacaaa cagaagatca tgaagaaacg aacacttcat tggaagaaca aacaagtaat
    52921 cctctggatg acacagaaga aatccctatg ctagaggaaa caaaaataca cgaaaaagaa
    52981 aaagtaactc cgacgaatac gaatactgca gaaaacaccc atgcttacga aataactgaa
    53041 aatcccgggt atttagaagg aaaagagcaa gatcatcaag atatcgtcag tttgattgaa
    53101 acagcggaag cagaattagc ggatttacgt atgagaaaag atttgatcga atcagatttt
    53161 ccacaattat tagaatcata tgatcgtttt atattgaatg aagaaccaat ctccacgatc
    53221 ggaaagacaa gcctgatcga atacttcacg tatgaattac tgaaaccgtt gaatgatatt
    53281 ggtctggaac ggtcaaattc agcttatgac acatggaaaa caagacattt ttcgatcaat
    53341 tatacgttga atggacaaaa cgaattagtc ttttcctttg tattaccgac catcgatcaa
    53401 cgataccaag tggaatcgat gagtctccta aacgtatcac cagagacaat ggaaatcaat
    53461 gtccaagatg accgggtgct ttctttgatc cgacactggt cagtggatcg agtattctct
    53521 gcagggcaga tcacgatatt caatcacaaa ttgaatcaat tattagccca tgcaagaaca
    53581 cttggtttta ccgttaatca gacattacta gacaatacga agccacttcg tttgaatctt
    53641 cagagcgaat ttgaattgac gaatgaagta ttggatgata tttttatcgt ggcaatgaaa
    53701 aatccatcct acgatatgga aaagatcaac gaaactactt atcaagtacg attagataaa
    53761 ggacagtctt taacgataac aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct
    53821 ggagactacc cacgatctgt cattgatttc tttatcaatt acgaattctt ggtcccatta
    53881 atggtgagaa agtcctgatt ggataagttg gattaccaga tgtcattcag ataattctgt
    53941 tccaaaggac ttgcggcagt tatccagaat aattgtcaca agtcttttct gtatgtacta
    54001 aatggacgtg aataacttgc tgatgaagga gggatttcat taggaatgtc cacaaataaa
    54061 atagcacagc ttagcttatt gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt
    54121 attccgaatt tccagccaat gactgctatt tttttattta tagtacttta cttaagctta
    54181 aaagatgcgt taattgtcat gagtctttct attgcgatca gcagttttta ttttggtgtt
    54241 ggaccatggg tcatcggcca atggatcagc tatactgttg ttctgagttt gttttcaatc
    54301 gtctgcttga gaaaaacagt gcaaaggaat ctatggttaa agggagtctg tttttttctg
    54361 gcgggcatgg tttacggaat aggtatgacc gtttttgata cgctgttata tcgtttgcct
    54421 caaccttgga tctattacct tcaaggggtg tcgtttgatt tgatgcacag tataggcaat
    54481 gttgtgttct ttcttgtttt tctgccagtc gtcaaacgtt tttacaatca ttcaggagga
    54541 aaaaatgaaa aaaataattt atagtttgct tgcaatgagt gtcagtatac tcattttgac
    54601 aagttgcagt acaacctata cacaatcggc ttccaaccaa aaggaggaag taaaaacatc
    54661 gcaaaaagca acaatcacgt tacaagaaaa tggtcaaaac ttttcggaaa aagaagtgtt
    54721 cttcaaaaaa ggcgatgatt tactgagtat tttgaaacga aattttgaag tcaaagaaga
    54781 taacggcttc atcacttcgc ttgaagggca ctcacaggat gagaaaaatc aactctattg
    54841 gatgtttact gtggacggga aaagtgctac tactggtgcc aaagagatca aattgaatga
    54901 tggacaatcg gtcattttca atttgtctaa actataagtt tcataaaaaa ctgttaaagg
    54961 gcgaatattg ccataattca gtgagttcag ttgatgaaag aaggaaaata acatggaaaa
    55021 ttttaaaaaa agcggaatgt tttccgtaat tgctgctcta ggggctaata ttctagttgc
    55081 tatcagcaaa tttgtcggct atgcaatcag tggcagtgcc gccatgctaa acgaaagcat
    55141 ccacagtatc gttgattgca gcaatcagat tttgctgtta ttcggtgata aacaagccaa
    55201 taaaggacag agcgagttgc atcaatttgg tgaaggacga gcaaaatact ttttcagtac
    55261 aatcgtcgca atgatgcttt tcttcggcgg tggggcactt ggggtgatgg aagcttttga
    55321 aaagttgacg catccatccc atgaagtggg taatccgatc attgttatag caatactgtt
    55381 atttggtttg gtaatcgaag gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt
    55441 gaatactgaa aaactttcca cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact
    55501 gattattttt acggaagact tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac
    55561 aatattgact tatgcaaccg ggattgctgc atttgatgcg atcagcggat tgctgatcgg
    55621 tttcttgctg atgggagccg ccattttcct agcaaaagaa ttttatagtc tgatcatcgg
    55681 tgaaagcgtg acagcaagcg atttatcaaa aatcaagtta gcttttgaac gtcctgagat
    55741 cagtcggctg atcgatataa aaacagttca tttaagtcca acggagattt tagttgcagc
    55801 aaaagcagat atcgttggta acaaagagaa tgaagcttac tcggtcatca atgatattga
    55861 aaaaaatatg cgtcagtctc ttcctgacaa gaagatgtat atatacattg aaacagataa
    55921 atatgaccca aattattctc gtaaataaaa cgtttattca tgaattgaag caaaaatgag
    55981 aaaaatataa tcttgttttt aaataaaacg gctacaattt agcgaattta tgttacaatg
    56041 agtccgctgt ttatttgttg taattaaagg agatatttta atgaacaaaa agaaaagtgc
    56101 gccatggctt caagctttgc atgcagtgat gccactttgt ataagctata taccagtagg
    56161 attagcttgc ggagtattgt tgcaaaaagt tggatttgat cctctcttat caggactgtt
    56221 atcgattctg gtcttttccg gtggcgcgca gtttttagtt gcatctatgc ttacgacgca
    56281 agcatcattt gcaacgacat tattgatggt tttcttttta gaattacgct atacattatt
    56341 aggagcaagt cttgccggtt ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc
    56401 tcaatcactg aatgatgaaa actatgcggt gaactactta aagttttcaa ctgacccttc
    56461 ctggaataaa agaaaggctt tatatgtcaa ttggttctca atgactgcat gggccggtag
    56521 caatatgatc ggtaattttt tcggctcggt gattcgagta gatgctgacc ttgtccattt
    56581 tgctttgacg gccatgttca ttttcatgtt tatcatgcag atgaaaagcg cactactgat
    56641 ctttacaggt ttattctcag gcgtattagg ggtcgtattc atggtattat tccaaaatac
    56701 tttcggattg attgcagcaa cagtcattgc ttcatttgcc ggatttatgc tggagaaggc
    56761 attcaaaaaa gaaaaagcta agaaactaag agaccgcggt aaagatgtca atgagccgct
    56821 cttcaacttc ccggaacagg aggccaatca ccatgactaa actatatttg ctacttttag
    56881 ttgcttgtct gttcatcgtg gcatatatcc cgcgcttgtt cccgatgctt tattttactc
    56941 atcgaaaagt gccatcctgg ttcagtgact ggatgaaata tgtaccggtg gcattatttg
    57001 cggcgttagc ttttaaagat gttttcatta ctcatgaaca tctggatatt gcttggaata
    57061 tcaaaatagc tgcaatgatt ctagtagcag gcgtggctta caaaacacgt tcgatggcgt
    57121 tgtcagtttt gacaggactt gcgtctgtct ttttgctgtc tatgctttaa actgttttat
    57181 tttattaaaa acagagattg cgacataagt tgttcaaact gatctatccg aactgcattt
    57241 gcaacagtca attctggatg caagtttatt cgtagggata aaaaagatta gtgttggctt
    57301 atgatgcaag ctctttttta ttttaggaat tagagaggga tttgtccttc tatgattttt
    57361 tgctataata gggaagatgt ttattttatg aaagaatagt tgagggaatt taaatgaata
    57421 aaaaattgat gcaaggaaca ttttggctaa cctttgctaa tcttctatgt aaagttttag
    57481 gggtagtcta tttgattccc tggctaagta tgatggggaa caatcaagat ggtatgcttg
    57541 ctacgacatt gtataatgtt ggctatcttc cttatggatt gttcctgatg cttggtacag
    57601 tcggatttcc aaatgcgatt gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg
    57661 ctgcttgtcg gctgattttt agaagtacga tcaatatcat gtttgtcatc ggtatcgcct
    57721 ctgctgcact gatgtattta tttgctcctt tactagccgg aatcagtccg attgctaacg
    57781 ttaataatgg gatactagct atcagaagtt tatgcccatc tttgattgct atcccaattc
    57841 ttagtgcaat gagaggatat ttccaaggaa aaaatgatct gcggccttat ggaacttctt
    57901 tgattatcga acaggctgtg cgagtgatcg ttatcttggc tggtacttat tacttgcgcg
    57961 tattgactga cggaaccata ttagaagctg tattgatcag tacagtcgct tctttcttcg
    58021 gtgggttagc tgcgattatc catatgtata tcgtcggacg cagaaaagac ttcttcgaac
    58081 ttaaagattt tttgatttcc agccgttatt tcgaaagaga aaaccgatct gcctctgtct
    58141 ctatcattcg ggaaacacta ccgtttattt ttgtcggctc agtcattact atcgttcaaa
    58201 tgatcgacca agtaacgatg aaaccactgc ttcacttttt ccgtccagag atcgcagatc
    58261 agcaactgga attcttattc agccgtgctt ctgtcaatcc aaataaattg acattgatcc
    58321 tgatctccat ggtaggaaca gtagctataa gcagcttgcc aatattaagc acgatgaaga
    58381 aaaaagatcg gatacaaatc gaaaaaacag taggagatag tttttcgatt gcgttattga
    58441 ttcttcttcc ttcattgact ggaatgtctt tattagctgg accactatac acattgtttt
    58501 ttggctatga tcctgaaagt gtcggatatt tccaaatggc tttgctagct tctttgtttt
    58561 tctctttatt cacgatccta tctacgatgc ttcaatcttt gaatcaccat cgttttgcga
    58621 tccgattaac tgtagaagca attatcctga aagtcatctt ccaagtaatc ggattaggat
    58681 tagttggcgg atacgggatg agcttatcaa atggcttagc attcggtatt gtctttgttc
    58741 gcggctatca ctttatgtgc aaagaatacc gaatagcacc tttagcaaag atcagcaatt
    58801 ttttcttaaa aactttccgc tccaccctga ttatgcttgc agtatgtttt gccgttttct
    58861 ttttgctgaa tcagcgattg tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg
    58921 ctgtaggaag tattggcggc ttagtatttt tattttctca attcggaaaa aatgggtttc
    58981 gaatgttgaa aaatgttaaa catcatagca gaagcaaaga ataggttaac taatcgtttt
    59041 ttgctgtcac ttgaaaaacg gcaactcgta tgattacgct tgtcgttttt ctattttcca
    59101 attttagttt catatctaat cttacttatg atatagttat aaggtctgga aaaattcttt
    59161 gaaacgagga aattaaacac atgaaacttg cagtagttac agatagttcg gcatatctgc
    59221 cggaacgtat caagaaccat cccgacttat tcgttattcc aatccctttg attatggatg
    59281 ggaaaattta taacgaagga atcgatatcg aagcggatga atattatgga cttttaaaaa
    59341 acagtaaaga ttttcctact acttcacaac ctgtcgtagg ggaagttttg ggtttatatg
    59401 aggaattgaa agagaaaggg tatgacacga ttttaagtat ccatctttct tcagggattt
    59461 ctggctttgt caacacatta tttgcgatga aagatgacat cagcggagta acggtcattc
    59521 cttatgactc taagatcact agtatgccga tgggacacat ggtagaagct gccttagact
    59581 taaatgatga aggtcagtcc cttgatgaaa tacttgcaca tatcgatcga atccgagata
    59641 atacctacgc ttatctgatt gtagatgatt tgaacaattt agtacgagga ggacgtttga
    59701 caaatggtgc agcgctgatt ggcagcttat taaaaatcaa accgatcctg acattttctg
    59761 aagggaaaat tgttttattc gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg
    59821 aagaaattat tggtagaaga aataacgaaa tacaaccgcc agtcaaactt tacgtgatac
    59881 atgcaaataa tttagaagta gcgattgcgg agaaagagaa attgcagaaa aaatatccaa
    59941 atgcagaaat cgaaattggt catttcggac cagtcattgg tacacattta ggtgaaaaag
    60001 ccatcggcat tgccatttca gcgcaataat tccttttatt caaaaaacat accaaaaata
    60061 ttccaacatc atcttggact ttctacatgt tactaatgag cgtgtaaaag aacgagatgg
    60121 tgtttttatt tctatcaatg aagctctttt tctttgcaat cctcacttca atcctctata
    60181 atgtcgcata tagtttagta aatgagtaaa ctactaattt acttatttac taaaaataaa
    60241 aggatgagga gttaaaataa tggaaatacc aacaaattta aaacataaac cagtaatcgt
    60301 tgtagaagac tatgataaag ttgatggaag aaatgcatta aatacggatg caaaagggct
    60361 ttcactgggc cttgctcagt ggaatgatcg tggaaaagtc gatatctcag gcaaggtatg
    60421 gcgacatact ggagaaaaat ggtcacgaca gtcggaagaa ttaccaatca cacgtatttt
    60481 agatttagcg atcttagctg cacaaggaag tttatacttc caagatgctt atcgttatga
    60541 aaaattttat gatccagaaa atccagtcgt agacatcatt ggattacaag ggaaccgaat
    60601 gacggtagaa gtagatacca aaaatccaaa gatcgatgaa gacatcattt tgtattacga
    60661 tacactacaa aaagacggag aactgattgg ggaacgattc cgtattttaa aacgtctttt
    60721 agatgagcta ggatattaat agacagagat gatcaaaagg ggaagagaag catggagaat
    60781 ttctcactaa cagaaaaccg aaccttttca cggctagaaa agcaaatgat ctggaagaaa
    60841 ccgaaaagcc atcggacaag tgcagaagaa tggcggatcg cctccgaaat caaagggaat
    60901 tggcaagatc ctgaaatgaa aatcagtaac gtacttttgg aaggagatgc cgggtcgggg
    60961 aagacacaat tagccaaagc actatctgct gatctgcaac ttccctatac aaaagtgacc
    61021 tgttttgcag atatggacaa atcagatgtt ttcggagcct tattacctgt agtagattct
    61081 aataaggaac aagaccaaga attactagaa gctatttacc aaacagatac acttgaagct
    61141 gtactgacac tgatccagaa ccattacggc attgatcctt tttctgctaa agaaaaatta
    61201 gcagagctag ttcaaaggat cgaagctgat tatccagatg caatccatta cagctattac
    61261 ccttctgaaa tcgttcgagc gattgaaaaa ggatatttat tagagattca agagccgact
    61321 gtcatccgag atgcctctgt tcttgtggca cttaactcag cgctcgaacc aaatgggatg
    61381 ttgaatttac cgacaggaat cattcgtcgc catccagatt gcgtcattgt gatcactaca
    61441 aatcgaaatt accaaggaaa tcgcccatta aatgaatctt taagagatcg gatgcaacat
    61501 gcggaaaaaa tggatcttcc tgccttagaa gtcatggcag aacgtgcgat ggcgaaaact
    61561 atgatccaaa aacaagaact tctcttaaag atggcagaga ttatccgttt gttggatgaa
    61621 actgcaaaag ctaacgccat caaaggtgta gcaggaatgc gttcttattt ttattgggca
    61681 aatacctata agcaaggaca agatttactc cagtcgattt atccaaaagt tttgtataaa
    61741 ttatccactg attcagatga attggcaatt cttgaacaag ctttgtctga cagtggattg
    61801 ctactggaac ttcaggacat attgagaaaa gaaaaatggg gtcatcctca atctgagcat
    61861 acaaaaggaa gaaccattag cgccgaagaa gcaaatgaac gaaacatcac tgaaaatgct
    61921 gaagaaattc tgcgcttaga tgaaaagaaa tcgttgtctg ctgaagaacc aaatgaggga
    61981 gaagagaaac aagaagaaac aatagaaaaa aacgagattc ctcctcaaat gcaaaaagaa
    62041 cgcagtagcg atatcgaagg aaaaaatcaa aaagaacaac aaacaggaga tgattctcaa
    62101 gaaatgtctg cttctgacat ggaagcacat gataaggaaa tcaagaagca actgaataaa
    62161 gaagcgcgac aaatcatgaa ggaaacgatt catgaaaaag aaggtctcat cgttcaccga
    62221 ccagctttcc aaggaaaaac gccggaagca cgtgagctac acatgcaagt aatgccaata
    62281 gtagaatcac ttagccggca aattttagag ttactggata atgaacaaac agagacgtac
    62341 caaaaaggaa aatacgaagg tcagcggttc aatgcaagca aagtagcata tggtgatttg
    62401 agaaattttg acaaaaagaa cccgccacat gagcaacctt ctttagcggt agctgtacgg
    62461 atcgatgaat ctgggtcaat gatacgggat gatcggatcg aagcggcaaa aaaagcagcg
    62521 attgccattg ccgaattttc taagaaagtc gatatcccat tgttgattta cggagataca
    62581 gcagaccgtt cgtcaagaga aaagacttct ttgttttctt ataaagaatt tgaagacgga
    62641 tttcaatggt taaatgaaaa attagtcact atgaaaccaa ggcaaaacaa tcgtgatggt
    62701 gctgcacttc gtttagcagc agaaaaatta aacagacaat ccgcaacaac caaactgttg
    62761 cttaatatta gtgacggtca gccaaaagcg ttgccggatt atacaggcaa aaaagcaaaa
    62821 gaagatatcc agtctgtttt gaaagaatat gaacgtcaag gaatcctatt tgtctccgca
    62881 gctattggcc aagacaaaga agaaatcaag tcgatctacg gtgaaagtcg ttttgtagat
    62941 attactgact tgaatgaatt tcccaagcaa atgattcaac tgattgcccg ctatctatga
    63001 taaaat
//
LOCUS       DACJGB010000007        61941 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.007, whole genome shotgun sequence.
ACCESSION   DACJGB010000007 DACJGB010000000
VERSION     DACJGB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.007"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            275..1111
                     /locus_tag="IYI34_000479"
     CDS             275..1111
                     /locus_tag="IYI34_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAR0558377.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            1175..1606
                     /locus_tag="IYI34_000480"
     CDS             1175..1606
                     /locus_tag="IYI34_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0558378.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(1668..2990)
                     /locus_tag="IYI34_000481"
     CDS             complement(1668..2990)
                     /locus_tag="IYI34_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAR0558379.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3144..4784)
                     /locus_tag="IYI34_000482"
     CDS             complement(3144..4784)
                     /locus_tag="IYI34_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAR0558380.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5117..5587
                     /locus_tag="IYI34_000483"
     CDS             5117..5587
                     /locus_tag="IYI34_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAR0558381.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            5809..6912
                     /locus_tag="IYI34_000484"
     CDS             5809..6912
                     /locus_tag="IYI34_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAR0558382.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7196..7876
                     /locus_tag="IYI34_000485"
     CDS             7196..7876
                     /locus_tag="IYI34_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAR0558383.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            7966..9000
                     /locus_tag="IYI34_000486"
     CDS             7966..9000
                     /locus_tag="IYI34_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAR0558384.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9014..9349
                     /locus_tag="IYI34_000487"
     CDS             9014..9349
                     /locus_tag="IYI34_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558385.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            9333..11813
                     /locus_tag="IYI34_000488"
     CDS             9333..11813
                     /locus_tag="IYI34_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558386.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            11869..13428
                     /locus_tag="IYI34_000489"
     CDS             11869..13428
                     /locus_tag="IYI34_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558387.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            13425..14789
                     /locus_tag="IYI34_000490"
     CDS             13425..14789
                     /locus_tag="IYI34_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR0558388.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            14960..16030
                     /locus_tag="IYI34_000491"
     CDS             14960..16030
                     /locus_tag="IYI34_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558389.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16045..16713
                     /locus_tag="IYI34_000492"
     CDS             16045..16713
                     /locus_tag="IYI34_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558390.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            16733..17311
                     /locus_tag="IYI34_000493"
     CDS             16733..17311
                     /locus_tag="IYI34_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0558391.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            17712..18623
                     /locus_tag="IYI34_000494"
     CDS             17712..18623
                     /locus_tag="IYI34_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR0558392.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(18711..19628)
                     /locus_tag="IYI34_000495"
     CDS             complement(18711..19628)
                     /locus_tag="IYI34_000495"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAR0558393.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            19830..20267
                     /gene="zapA"
                     /locus_tag="IYI34_000496"
     CDS             19830..20267
                     /gene="zapA"
                     /locus_tag="IYI34_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAR0558394.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            20326..20874
                     /locus_tag="IYI34_000497"
     CDS             20326..20874
                     /locus_tag="IYI34_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAR0558395.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            20989..23349
                     /locus_tag="IYI34_000498"
     CDS             20989..23349
                     /locus_tag="IYI34_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAR0558396.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            23436..23750
                     /gene="trxA"
                     /locus_tag="IYI34_000499"
     CDS             23436..23750
                     /gene="trxA"
                     /locus_tag="IYI34_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAR0558397.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            23932..25710
                     /gene="uvrC"
                     /locus_tag="IYI34_000500"
     CDS             23932..25710
                     /gene="uvrC"
                     /locus_tag="IYI34_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAR0558398.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            25855..26754
                     /locus_tag="IYI34_000501"
     CDS             25855..26754
                     /locus_tag="IYI34_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558399.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            26747..28009
                     /locus_tag="IYI34_000502"
     CDS             26747..28009
                     /locus_tag="IYI34_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558400.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            28170..30068
                     /locus_tag="IYI34_000503"
     CDS             28170..30068
                     /locus_tag="IYI34_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAR0558401.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(30398..31117)
                     /locus_tag="IYI34_000504"
     CDS             complement(30398..31117)
                     /locus_tag="IYI34_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAR0558402.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            31454..31786
                     /locus_tag="IYI34_000505"
     CDS             31454..31786
                     /locus_tag="IYI34_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558403.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            31773..33776
                     /locus_tag="IYI34_000506"
     CDS             31773..33776
                     /locus_tag="IYI34_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAR0558404.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            33795..34265
                     /locus_tag="IYI34_000507"
     CDS             33795..34265
                     /locus_tag="IYI34_000507"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAR0558405.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            34286..34870
                     /locus_tag="IYI34_000508"
     CDS             34286..34870
                     /locus_tag="IYI34_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAR0558406.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            34885..35871
                     /locus_tag="IYI34_000509"
     CDS             34885..35871
                     /locus_tag="IYI34_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAR0558407.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            35861..36172
                     /locus_tag="IYI34_000510"
     CDS             35861..36172
                     /locus_tag="IYI34_000510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAR0558408.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            36194..37975
                     /locus_tag="IYI34_000511"
     CDS             36194..37975
                     /locus_tag="IYI34_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAR0558409.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            37968..39344
                     /locus_tag="IYI34_000512"
     CDS             37968..39344
                     /locus_tag="IYI34_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAR0558410.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            39351..39986
                     /locus_tag="IYI34_000513"
     CDS             39351..39986
                     /locus_tag="IYI34_000513"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAR0558411.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            40016..41371
                     /locus_tag="IYI34_000514"
     CDS             40016..41371
                     /locus_tag="IYI34_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAR0558412.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(41563..42957)
                     /locus_tag="IYI34_000515"
     CDS             complement(41563..42957)
                     /locus_tag="IYI34_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAR0558413.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            43049..44020
                     /locus_tag="IYI34_000516"
     CDS             43049..44020
                     /locus_tag="IYI34_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAR0558414.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            44017..44799
                     /locus_tag="IYI34_000517"
     CDS             44017..44799
                     /locus_tag="IYI34_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAR0558415.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(44968..45339)
                     /locus_tag="IYI34_000518"
     CDS             complement(44968..45339)
                     /locus_tag="IYI34_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAR0558416.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            45473..45916
                     /locus_tag="IYI34_000519"
     CDS             45473..45916
                     /locus_tag="IYI34_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAR0558417.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(45959..46768)
                     /gene="yidA"
                     /locus_tag="IYI34_000520"
     CDS             complement(45959..46768)
                     /gene="yidA"
                     /locus_tag="IYI34_000520"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAR0558418.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(46787..48154)
                     /locus_tag="IYI34_000521"
     CDS             complement(46787..48154)
                     /locus_tag="IYI34_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR0558419.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(48138..48968)
                     /locus_tag="IYI34_000522"
     CDS             complement(48138..48968)
                     /locus_tag="IYI34_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAR0558420.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            49131..49568
                     /locus_tag="IYI34_000523"
     CDS             49131..49568
                     /locus_tag="IYI34_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAR0558421.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            49644..50264
                     /locus_tag="IYI34_000524"
     CDS             49644..50264
                     /locus_tag="IYI34_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAR0558422.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            50453..50959
                     /locus_tag="IYI34_000525"
     CDS             50453..50959
                     /locus_tag="IYI34_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAR0558423.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            50976..51875
                     /locus_tag="IYI34_000526"
     CDS             50976..51875
                     /locus_tag="IYI34_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAR0558424.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            51962..52846
                     /locus_tag="IYI34_000527"
     CDS             51962..52846
                     /locus_tag="IYI34_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAR0558425.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(53038..53109)
                     /locus_tag="IYI34_000528"
     tRNA            complement(53038..53109)
                     /locus_tag="IYI34_000528"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53074..53076),aa:Arg,seq:ccg)
     gene            53169..54503
                     /gene="rpoN"
                     /locus_tag="IYI34_000529"
     CDS             53169..54503
                     /gene="rpoN"
                     /locus_tag="IYI34_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAR0558426.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            54706..55743
                     /locus_tag="IYI34_000530"
     CDS             54706..55743
                     /locus_tag="IYI34_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAR0558427.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            55784..56785
                     /gene="gap"
                     /locus_tag="IYI34_000531"
     CDS             55784..56785
                     /gene="gap"
                     /locus_tag="IYI34_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAR0558428.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            56895..58085
                     /locus_tag="IYI34_000532"
     CDS             56895..58085
                     /locus_tag="IYI34_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAR0558429.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            58189..58944
                     /locus_tag="IYI34_000533"
     CDS             58189..58944
                     /locus_tag="IYI34_000533"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAR0558430.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            59060..60358
                     /gene="eno"
                     /locus_tag="IYI34_000534"
     CDS             59060..60358
                     /gene="eno"
                     /locus_tag="IYI34_000534"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAR0558431.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            60757..61896
                     /locus_tag="IYI34_000535"
     CDS             60757..61896
                     /locus_tag="IYI34_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAR0558432.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        20465 a        10838 c        13056 g        17582 t
ORIGIN      
        1 aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata
       61 agttcaacca aacgaaaatt atgaggagct atttttgttt ggctgttctt attacttgat
      121 gcaggacagc tttttcacaa cctcttttcg tttagcacta aatttcctta aaaagtagaa
      181 aagctttcga agcctcgttt gtttctttta ctgtataata aaagaaaaga gtaacaatcg
      241 ctaaaagata taaacaagag aaaggaaata caagatgatt ggtctgattt attttgtggt
      301 gatcgttctg gcgaacacag ttggcgctat ttccgggatg ggtggaggcg tgttgatcaa
      361 accgattctt gatcttatcg gcgcccattc tgttgcaggg atttcttttt actcaacagt
      421 tgcggtattc acgatgtcca ttgtttcgac tgtgcgacaa gtatcaagcg ggaaatcact
      481 gaattggcag attgtcggtt gggtatctag tggagctgtt gttggaggag tagtcggcaa
      541 cattgtattc gaagtttttt tacagttatt tgaaaacgag aagcatgtgc agctgatcca
      601 aatttttttg acggtattga ccttggtttt tgcgttcttc tacaccaagc atcatcagcc
      661 aaaatttcat ttgacgtcat ggacttggta ccttatatgt gggggggttc tgggcttttt
      721 agctagtttt ttaggtattg gtggtggacc agtcaatgtt tctttgttga tgctgatgtt
      781 tgcattgcca ataaaagaag caacactata ttctctatct acgattttct tttctcaact
      841 ggcaaaattg gtaacgattg ctttaacaag tagctttatg cgctttgatt tatctatgtt
      901 gttttatgtt atccccgctg ctgttattgg aggtttgtgg ggagcaagat ttagcagggt
      961 tttgtctcca aaaaaagtga cctttatttt tcaagtaatt gtcctcgtgg ttcttttggt
     1021 caattgttac aatgcctttg tcatcttaca aacattagga gaatctagac taacgcaagt
     1081 caaaatcact gaaacatatt gttctaaatg aaaaaaggag tatactgtga taaaaaaatt
     1141 catttaaaaa aataaaaatt ggctggtgat ttggatgttg cgggaaatgc gtttaggaga
     1201 tgcagaagca ctacgagaaa tcaatgcgga acaattagga tatgatgtac cattaaccgt
     1261 tacggtgaga caaatgaaga aactgttgaa ggagccagaa aaaaattttt tcttggtcta
     1321 tgaagatcct acttctaaaa aagcagctgg ctatgtacaa gcggagtcct atgaatcgat
     1381 tttcagcgat ccgatgttca atattatggc acttgctgtg tctaaacatg ctgaaaaaag
     1441 aggaatcgga aaggccttga tgactggcgt agaagaagcg aaaagacgag gaatatcagc
     1501 aataagactt aattctgcag aatacagagt agaagcacac cgattttatg aacgcatcgg
     1561 ttatcattcc gataaaatgc aaaaaagatt tttgaaaatc ctgtaaattt ttatcaaaaa
     1621 atacctggtc atcaagccga tacttgatga tcaggtattt tttttgacta agaaagtaat
     1681 tgcatcaaga aacctgagat gatcaaaacg atggcaccac caagccgatt gcccatttga
     1741 gcaaaagcaa taagttccat ccgattagct gcagataaga cagctacatt tcctgtacca
     1801 cccatcgagt tgttgcataa gccagcagta atagttgatt cgattggata taaaccaaac
     1861 aaacgaccga caaatcctga aactactgaa atcgcgataa cacttgtcaa acataagaca
     1921 acaaattgcc atgtcaaagc agaagccaat acatttagat tcaataacgc aataccgata
     1981 ccggcaagaa cagctgctgt caagtttttg acaatcaagt tgttgaacat gatagctgca
     2041 tcttcgtaat attctggtaa taggtttgcg attttacaga taacaacgat aatgatcatg
     2101 aatgcgtaag catggacttt aggaacaaaa tagttacaga tttgtcctaa gatgaagaaa
     2161 gtcaatgata ccatcaaacc gacacctaat tgtggaatgg aaggttttac atttctaggt
     2221 tttccatcgc caaggtcgcc tttttccatt aatttaccat taccgttgaa ttttggcatt
     2281 gattgtcccg cacgagcaac taacgctgca ccaatgatag ctaggacatt tgtcattgct
     2341 gtcgcaggaa tcaggcggga aatgatagaa gaagcgtccg tacctaaaac atttgcataa
     2401 atcgttgata aaggtacagc tccggctcca acaccaccag ccattgcggg caatgagata
     2461 tataaaacgg aatttgcaaa accatttccg ataagcatac ctacgagtcc tacggcaaaa
     2521 aaggagacga ccattgaaat aagtgcaacg ggaataaaac gaacagaagc tttcatcaat
     2581 agattacggt tcataccaag gatgctacca gtaatcaagg cagcaatata aaagtctaaa
     2641 aagcccatat tgctgacaaa gttttctgtg gattttacta catactcagg taagatccca
     2701 aatgtagcaa tcgcggcaga ggcaaagatc gcaaaaacgg agccaccacc taaataactt
     2761 ttgacgatag ggatttttgt tccgataaaa tgaaataaat taccgaaaat gaccaaaaca
     2821 gcgattgggc caatcatatt cgtcggtaat tttcctaaag ccattgcaat gatcaggaca
     2881 aggaccatga tcaaataaag gggcaaactt acgccggtaa tttttgttga ccaaaaccct
     2941 tcttttttct tttcctcaac tgcagttgcg gttgttgttt tttgtgccat ttttacagat
     3001 cctttcttat tctgtatcgt atgaaaaaag ccaaagagaa gcggcaaaag agcagtagtt
     3061 ggctggctgc tgttttgccg cagtcgcttt tttcttttta gacgtcatac aaagtttctg
     3121 tatgttcttt tactataaca atcttaatct tctaaagatt tatattcagg aacccattta
     3181 gcagattcaa cagcttcttt gatatccgtg atttcttcac gattcaattt ttgtgcaaca
     3241 actgattttg cgacagtttc agcaatcgtt tgagagaatt cagtgatttt tgctactgga
     3301 ggcaagatag ctgctcctgg ttttgttaca tcaacgattc cgcctaatgc tcgactagct
     3361 tgagaaatga tttcagcatt tactcgtgta gctgttgaag caatcaaacc aagccctaaa
     3421 cctgggtaca tcaacgcatt atttgcttga ccgatttggt aagttacacc gttatattcc
     3481 acatcagcag cagggatacc tgttccaact aaagcttttc cgtccgtcca ttcaatcaaa
     3541 tcttttgctt ttgcttctgc caattttgtt ggattagaca aaggcatgat gattggacgt
     3601 ggggtatgag ccgccatttc tttcacgatt tcttcagtga atgctccagg ttgggtagaa
     3661 gtaccgatca tgatcgttgg atggattgct tttacaactg cttctaagtt tgtcaattca
     3721 tcagcattag tgaattctga gcgtttacga gcgaatggga tttgaccagg agtcaatcct
     3781 tttgtatctt caaacaataa accctgttta tctacttgat agaaatgttg gcgtgcttct
     3841 tcttcaggta caccttgacg gatcatttca tctaaaagga tgtttgcgat acctacacca
     3901 gcagtaccag caccaaatgt caagatcgtt tgatctttca attgctcacc tgatatattc
     3961 aatccaccta atacaccagc tagtacaacg attcctgtgc cttggatatc atcattgaaa
     4021 gtagtgattt tatcttcgta tttttctaag atcgttgcag catttcctcg tccaaaatct
     4081 tcccaatgta ataacaattc tgggaataat tcagtagctg attgaacgaa ttgatcaatg
     4141 aattcgtagt aagtttcgcc ttctacacga gcatgacgat tccctaagta taatggatta
     4201 ttcaataatt cttcgttatt agtaccagcg tcgattgata caggcaagac ttgtgcagga
     4261 ttgataccag ccgcagcagt gtagaccatc aatttaccga tagcgatgtc aacaccgttc
     4321 acaccccagt cacccatacc taagattcct tctgcatctg tcacaacgat caaacgaatg
     4381 tcgcgtccat ctgctgcgtt tttcaaactt gctttgatat tttctggttc gtcgattgaa
     4441 agaaatgcag catcttgtgg tttcaagaaa atttcgttgt attgttcgat tgaatccgca
     4501 acgacaggat cataaacgac aggcatgaat tcaactaaat gttcgcccat caatttataa
     4561 aacaatgtcc ggttcgtatt gaatagattc attaagaaga tacgtttttc caaatcggtc
     4621 tgtttgctta agtattgacc gtaagcttgg acagattgtt gttccaatgt ttggacagta
     4681 cttggaagca taccagtgat tccgtatttt gcccgttctt cttttgtaaa agctgttcct
     4741 ttatttagaa aaggattatt taataagttt agccctgtaa gcatgaatga ttcctcctaa
     4801 gttttaatac aaacgaagta taggagtcag atgaggatat agtcaaaaag agcaggtata
     4861 ataaatttta tttatatgag taatagagtc aaaagaaatc aaaatagcgg tatttgttct
     4921 ttttatatca acaatccttg cttgattact atgcgtatct cttatatgct aaagaaagaa
     4981 aagtagaaga ataaaggata tgttagatag ataaatagaa tctctgaaga ataaatcacg
     5041 tgatcgtgac gataaaatac acttacgtca ggtgataaaa aatacataag tagaaaacta
     5101 agaggaggaa cagaaaatga aagaagttca attgatcaga aaaagcgaac tatcagaagg
     5161 tggttgcaat gcatgcggcg tagtagaagc aacaagctat acattaaagc ttggtgccaa
     5221 caaagcgata atctctgagt tgacagtagg cggtcttgtg gacagtctgg cgcttgctga
     5281 aggttttatt ggagaagata tttatgagat gttttcagaa atccgtcagt taaaaaaagg
     5341 ggaaaactgc atagaggttc atcatgagag cccgaatgtg agattcaaac gtggtgataa
     5401 tgagatgatc ttcaataatc atgtgtccga tcacaccgag ctatatggaa tcgtgaatca
     5461 aatactaact gagctttttg gattgggacc ttatgcgttc aaggaagaaa acggtaatcc
     5521 aaaattaaac gaagaatggc aagaaacgat tgaaatacaa agaaacaacc cgcacttatt
     5581 tcaataggca tccacagtat ttcgtaaaag ggactttgac acgacttttg tcaaagtccc
     5641 atcgtttttt atctatttgt gttctaaaca tttctttcag agtagtaaca actggaatga
     5701 ctgaaatgta aacagattat ttgcctgtat caatacggaa gagtaatata aagtgtgtaa
     5761 acgctatcat attattcatt caatttttta tagagggaag gcattgaaat ggaaaaaacg
     5821 tatcaagcag gtacaaatga aggaatagtg gattttatca atatggaaga tttagaaata
     5881 gccgcttcac aagtgattcc agctggtggt tatggttaca tcagcagtgg tgctggcgac
     5941 ttatttactt atcaagaaaa tgaacgggca ttcaatcatc agctgattat tcctcatgta
     6001 ttaaaagatg tagaattacc cgatacaact acacattttg acaaagaaac gctgactgca
     6061 ccaatcatca tggcaccagt tgctgctcat ggcttagctc atgtgaaggc ggagaaagct
     6121 tctgcaaaag gtgtagcaga ttttgggacg atctatacag caagttctta cgcttcttgt
     6181 acattagaag agataagaga agcaggcggg gagaaagcac cgcaatggtt tcaattttat
     6241 atgagcaaag atgatgggat caatttggat attcttgaag tggccaagcg taatggagca
     6301 aaagcaatcg tattgacagc ggatgcaaca gttggcggaa accgtgaaac agaccgcaga
     6361 aatgggttta ccttcccact gccaatgccg atcgttcagg cttatcaatc aggagtaggc
     6421 caaacgatgg atgctgtgta caaatcttcc aaacagaagc ttagtccaaa agatgtagaa
     6481 tttattgctg ctcattctga tttgccagtc tatgtgaaag gtgtccagtc agaagaagat
     6541 gtctatcgtt cattagaatc tggtgctggc ggaatctggg tatctaatca tggtggccgt
     6601 caactagatg gtggtccagc agcgtttgat tcgttgcaat atgtggcaga agcagtagat
     6661 aaacgtgtac caatcgtgtt tgacagcggt gtacgacgcg gacagcatgt tttcaaagca
     6721 atcgcatcag gtgcagatct tgttgctatt ggcagacccg ttatttatgg attgtcttta
     6781 ggaggaagta caggagtcca tcaagtgttt gatttcttta aaacagaatt ggaaatggtc
     6841 atgcaattag ctggaacaca aacagtagaa gatatcaaaa aaataaaatt gcgtgagaat
     6901 cgttttattt aagaaacaaa tgacgaaaaa aataggagtg cggcagatga tgccgtactc
     6961 ctatttttat agttcaagga tgtcactaat agaataattt gaaacgaaaa aataaaattg
     7021 aaatattttt tcatacttaa taaaaaagga agaatattca tgtaaacgat tttattgttt
     7081 ttttctcttt tatacaaaaa gggtcataat ttcaagtaag cggatgaatt catgaggatt
     7141 ttttgcatac tgtctctact atacttaggt cactaagaaa aaggagtgat ttattatgaa
     7201 aaagaacatg attgggacat tattactttc aacacttctt ctaggcggct tggcaacacc
     7261 agcttttgct gagggtcaag cgacttcaaa aggagatatt acctttactg aaccaacaaa
     7321 taccgtggag cctcttaacc ctactgatcc ttcaaaacca gtagagccag cagatccgga
     7381 aaatccagca actggccaga caggtccttt aacactggat gtagttcccg aattgccatt
     7441 tggtacacac gagattgaaa gcggaacaaa aacgtatcaa gtagacgctt caaaaaacga
     7501 tacaccttat ctccaagtat cagatcgtcg aggtgtaggt gcagatggcc aagcacaagg
     7561 gtggaacgtg acagtttcgg tttcagattt tgtaaacggt tcacaagttt tgcaaggcgc
     7621 tgaattagat tttggaacat ccactgttaa aagtacgtca gataacgaat ccactggacc
     7681 aactagtcaa actgtaactg ggttgagtaa agcaagtgca gcgacaccaa tatttacagc
     7741 agcaaaagat caaggtctgg gaacatggct ttctgtttat gatccagcaa atatcacact
     7801 gaaagtgccg aaagcagcag ctggaacatt cacggctgat ttgacttgga acctcgtggc
     7861 tggcccagta gcatagagta tgaaataaat tcaacaaact gcggtgaaaa tactataggt
     7921 gaaggatttt ttaccgcagt ttttgttgaa aggatgtggt ggacaatgaa tcaaagtaaa
     7981 cgactgtttg tttcgttttt ttctatttta agtctatttt tcatttttcc gtcaatcagc
     8041 caagcaaagg attcagcagg agactttgga atcaaaccgg tatttccaga gaaccaaata
     8101 gataaagcaa tcggttattt tgatttatta gtggcaccgg aacaaaatca gacactagaa
     8161 gtaattatca gcaattcttc agatgaagaa cgaacatttg aggtctctgt caatccggcg
     8221 gtaacgagtg atggtggaac gatcgattat agtcaaaaaa acccaacgtt agatgagaca
     8281 ttgccgtttg atgtccgtga cgttttgctc attgcgaaaa aggaaatcaa tgtatcagca
     8341 catgcagaaa caactgtacc tatagaagtg aaaatcccag caaaaagctt taagggacgt
     8401 gtattagcgg gtattcatgt ttcacctaaa gaagaagctg aaacagaaaa tgctacagaa
     8461 ggagcacaaa tcaaaaatcg tattgcttat aatctcgcag tcgttttaca agaaagtcaa
     8521 gagacgatag aaccggatct gaaactttta tcaggagatt tggacgaggt caacgcaaag
     8581 ccaactgttc agcttcgttt ccaaaatcct cagcctagga ttatcagcaa tttgatattt
     8641 acgagcaaga tcttttatga aaatcagctt tatattgaaa atacgtctaa tgcattttta
     8701 gttgcaccaa attcgaattt tcacttgaat cttgatttag ctggtgataa agcaaaagcc
     8761 ggcgattacc gcgcagaaat catagcaaaa tcaggcgaca gtaatgaatg gcgatttaca
     8821 caaaacttta cgatcaaaaa agaaaaagca caaaaagtaa atgaaaattc cgtttttgca
     8881 gtacaagaac aatctttccc atggctttat gcaggattgg ttgctgcagt tctagtgtta
     8941 tggttgattt tgctgttttt gtgggtccac aagaaaaaga aaaaaggaga aacaacatga
     9001 tgaggtgatt tcgttgaaaa catcgatctt attgggaggg attattgcaa gtttgctgtt
     9061 cttcagctgg caagagccag tccaagcaga taccaaaagt tcaggtacga ttacatttta
     9121 tgatctaagt ccaattccgc atccaagtgg ttcagtcaat ggcgcatcaa aacagcagac
     9181 acagctaccg atttataaag aatctaatca acatggcgta ttcccggaga cgaatgaaca
     9241 aaagatccgt tttctttatt tgatcattgg tatagaatta tggcttcttg cgtggttgtt
     9301 gttatggaga agaaaggaaa aggaggatga acatgttgct acaaaataaa ttttcatttt
     9361 ttcgctcctt cttttttact tcgctttttg ttttaagtat atttatcttt tcaccttcgg
     9421 cagaagctgt tggagcaacg cctcctgcca tttcttatga tccatacacg tatgaaaata
     9481 tcgtacctcc aaatggtgga cgtagtgcag tcaatggcgg gaagcttgtc caactaactg
     9541 gggctaacca attgcctaaa ttgactgcta cttatttgaa cgatggttca aatgatcctt
     9601 ggaaaggtgg cacggtccgt tttcattggg aaagccatgt gaaactaaca aatgtacttg
     9661 atttggtgaa tatcactgca caaggatttg catttgtggc gatcatcaat ttgccggaac
     9721 atgtatcaag cagtgatgta ttaaaagcaa ttgactggaa caacgcctac ttgtatattg
     9781 ccggtgcccc catcaaggtt actagcggaa gtttattatc agcaggaagc cataccttgc
     9841 gttttgggat ggggtcgtgg gatacaaata ctctattgac agatattatt gctattatta
     9901 cgcgaggcga attcaacgtg gacgatatcc cgcttcaatt tgatgttaca gctaatgtcg
     9961 cgaagatgac agaaaacgga gataaatatg atacgagtcc aaacaaaatt ttgaccaagg
    10021 gtaaattaca accttctatg tctaaaaagg ccgttttttc tgttgatttt tatgattcga
    10081 ataatattat ccaagatcgt atgttcaatt cgattttagc tccagctgca ggtagatttt
    10141 atcctaaatt aactggcgga aacttgaatc caggagcata tgcattttct gcaagtgcag
    10201 agataaatac gtggaatagt tacatctcac caagtgattc aacaggaaaa tataatacat
    10261 ttgctgatac cacagaatac gtggatggaa ataaccagaa ccttccaggc tctaccaata
    10321 gtcggacatt gacgatgggg ctgactgaag gacgttttga tcagttgcca tttagtcgct
    10381 ttgatcgcgt agtcaactat tttacaggaa aaaacgttac acaaggagca atactgactc
    10441 atactccagc aagcacaagt ggattgaatc agacagccaa tatcgtgtac agtgggaaag
    10501 atgcgcaagg cggattgctt tctcctgttg ctttaaaggt attggagcag tacaagctag
    10561 acggaacggt tttaccaaca aacttaacag ccgatagtca gtggggaaag ctcctttctt
    10621 ctgcatcgta ccaagtcacg gccaattggt cagcaactga actaaaaaag ggttcgatcc
    10681 attacaggct ttattcgaaa ggaacccaaa aattagcgac aggatggaca gatcaacttt
    10741 tccaaacgat ccaatcaaat aacgggggtg taaatacagc tacttctaca ctacctgcat
    10801 tggcttcagg gcaatattat tttgattatc gcttggtgga cgatgtactt actaacatgt
    10861 atccgtcttt tgcgtacaaa tggcagtctg aacaagctgg aatcaccact cttcctcaaa
    10921 taacagtggc taattttcca tctgtgagtg cagatagtca gctaaaaaac cttagcagac
    10981 aacctgagac aggagatccc ctgtctgctc tatcaggaga tacgattcaa gaaaatgtga
    11041 cattcacgtt aacaaaatct ggggatgcga taaccgacaa aaaaatcacg atctcacttc
    11101 ctgaaaatac tacttatatc aatggttcct taaaattaaa tggtacaact atcgcagata
    11161 ccggcattca acaaggagtt tctgtcccag cagatctatt gagcaaaata ggagatacca
    11221 ttcatcttac ttacaactat caattaaata ctgtagatac ttctttacaa tccgtcagca
    11281 tactgacaaa ggcagctgta ctttccagca atattacatt agcagatggt gctaagcttc
    11341 ctaacccagt tgtccaaaca tctgctaaaa caattcttgt ccccaaacaa gaattaacat
    11401 tagttaatgt accagatgat tttacatttg gtaacgatct ccccaaaccg ttgaagacaa
    11461 gttattatga atcaaaaggc gatttttctt ttgatgtgcg ggatactcgg cttccaagca
    11521 cgtctccttg gcaactgaca ggtacattga cttctttatt taagaatgat caaggtcaag
    11581 aattaagcgg aacaaaatta tacttcaatc attctggatc aaagcaattg attcagcaag
    11641 gtcaaaacac gttgatttat gagagtgatg gaacagctaa aggagaggtt ctcgttgact
    11701 ttcccgatac cgatggttta ctcttagagg taaatagcag tacgaatgct caacctggcg
    11761 caacgtatca aggcatggtc acatggaaac tgacagctgg gccaaccagc taagtgatta
    11821 gaatttgtat ggatctgtga taactgtcac agatcctctt tttcaaggat gagaaggaaa
    11881 cttctgctgt taaaatcaga aatgcttgaa ggagtggact tggaactttt cagtttgctt
    11941 gaaaaaaatg aacaacggca aatcaaactt ttccaacttt tattagagga aaaaggagag
    12001 gtttccgtta ataacgtact tacccgctta tcagttgatc gggctacctt gaaagaagat
    12061 ctgttttatt tacaagctaa cttggaaaat atcaaacaat tggaaattgt tatccaagaa
    12121 gggaatattt tattaatcag acatggaaat atagcagcag tcgatgttta ttatcattgt
    12181 tacttgcgct attctacaaa atatcagata cttctttatc tgttggaaaa tggctcattt
    12241 gatcggcaga aacttgtcat ggaacttaat atcagttcgg ctactttgtc tcgcagagtg
    12301 aaggaattaa atgattcttt aaatgagttt catctgaaaa taaaaagcgg gcggctgacc
    12361 ggtccagaaa gtcaaattcg ttatttttat ttccaattat tttggtttgg acgtccctat
    12421 cttgaaaaca aaaaagaata cagtgattta tcaggaaaca cttttttaga tctgctgcaa
    12481 aaagagttta gttttgctat tacagaagaa ggacgattga aatttgaaat atggctttcc
    12541 attatgaaga agcgaatgcg ctgtacgaaa aaatccaaac cagattatag caatcttcct
    12601 gttggattgt ttcaaacgac acaattttta ttggacctcc atacattttt gacccgttac
    12661 ttctttcaat atgcctttcc ttggaatcaa aatgaaacgt tggtttttta tcttttcatg
    12721 atcagtaact ttacattaga tacacataac tcgcatgtcc aggagttttt gataagcgaa
    12781 aaatacaaag acgacgtagt agctcaattg aatcgaacgt ttaaacaatc gatagaagaa
    12841 tatttctctt tttatgtttt tccagaagag atttcgcaaa agattgcgtt gacagtgatg
    12901 caaaatcacg ctcattttgc ctattttaga ggatggatga atatttttgg ccaagaagga
    12961 ttaaaacagc ggttaactga agtagaagat aaacaattcc tggttttatg tgaaagattg
    13021 gtcagacaaa catttgctat tgttgatttt actgaagtag acgaggaaaa tgcccgcttt
    13081 gaattatttg ctcgctatgc atctatttta cggacgctga tcaagcagtt agaattacgt
    13141 ctgcaaattg cttatgatct tccttatgaa aggctaatgg cggatatcgt gaaagaaatc
    13201 atccaggaac aattgctgaa tacgatcaaa tatgatttag tcgaatacga gaaagaaaag
    13261 cagtatgata tcattttgac tagccaattg aaagagtacc cgtcacagaa gtccgctaaa
    13321 gtttttgtat tcactagcaa cgaatataat tatgatttcc cctatttgaa ccaattttta
    13381 aaagaatgtt atttagaaaa attgaattta aggatgaata aaacatgaaa atcgtgatca
    13441 ttggtgcttc ccatgcaggc atcacagctg ccctaaatct aagaaaactt caaccgaaag
    13501 cagaagtttt actgatcgat gaagatcata aagatggact aggctatgtt tcaaatggaa
    13561 tcaaccttta tttaaaagga aagatacgta gtttagctga agttgcccat aacatgcgga
    13621 ctcttcaaga aagtggggca aaactgatca ccgaatggag agtaactgaa cttgatccag
    13681 acaaacatca gctcatcctc gcatccaaag aaggaaaaaa cgagaccata acctatgata
    13741 aattaatcgt agcgacaggt tcctctcctg tcaccttgta taaacaaatt gaagctgaaa
    13801 atgtctacac ctataaaaat ctagtgcaat ctaagcaagt acttgccgct ttaaaagaag
    13861 caaaagaagt agtgatcttc ggtgctggtt acattggatt ggaattagca gatgctttga
    13921 gaaataaagg atacattatt catctagttg attatatgcc gaatgtttta tcaagatatt
    13981 tcgataagga tatgatcaac agttttcaaa atcagctgca aactaagcaa ataaattttt
    14041 atccaaatga attcttgatc gattggaaaa aatcagaaga gaaagtagta tccgtacaac
    14101 ttctgtctca aacaataaag gcagatatgg tcattttttc tgcccaaact cgtcctaata
    14161 cgacattgtt aaaagaaaaa gtggctttgt atgaggatga gactgtgata gtcaatgaat
    14221 atctgcagac aagtgatccg gacatctatg cgattggaga tatcgtccca gtcagttttg
    14281 acaaaaataa acggcatctc tttttacctt tagttactcg agctgtacat atggcaagag
    14341 cagtagcgct aactttgagt ggtcagccaa ccgcttacga cctcagacaa aagataacag
    14401 cagcagtcat tatagactac tttttaggaa ctgtagggct gacagaagat gaagcgccat
    14461 tcttggaaca aaataccggt tcgtgttcag gagaatttga tctatttcca caatacgacg
    14521 aagaaaacaa aacagtaaat gcaaagctga tctaccatcc ggatacatta gaaataattg
    14581 gcggacagct gataagccaa gaattcttat tatcagattt gaacctgctt gcggatatcg
    14641 tgaaacacaa aactacgatt ccacagctag cagttgagga ttttggattt ctgtctgaat
    14701 acacctctcg ttttcactac ctcaatgagt tagcttttaa agtactagct aagagagcaa
    14761 atagcaatag agtagataaa agactgtgag aaatatcacg gtcttttatt gtgtggggat
    14821 ataataaata ttaatcaatc gagaataaaa aattcatata atgaaatagc ggaagaattt
    14881 tttgagcata atattcgcca aaaatttatc aagtgataaa attgttgtat aaaatgatta
    14941 tttcaggagg aggagttaaa tgtttttagc attaaatgaa attatgcatt caaagttacg
    15001 ttatgcgtta gttgcagggg tgatgttttt gatcgcctac ctggtatttt ttttgactgg
    15061 gttagcttat ggcttagctc aagataatcg aacagcagtc gacaagtggg aggcagattc
    15121 gattgtgctt tcgaaagatg caaattcaaa tctagggatg tccatgatta ctaagaagat
    15181 agcagaagaa gtcgaaggtg ggaaagtggc gtatcttgcc caaacacctg gagtcgtgac
    15241 tagtaaagac agtacagaag aaggaaaaat caatgttagc ttcttcggta tcgataaaaa
    15301 tcaattcatc atgcccaatc ttgttgaagg aaaagctttt gataacgatg atgaggcagt
    15361 aggcgatatt agtttgaaag aagaatatgg tctagccata ggagatacag ttaagttatc
    15421 tggtagtgac aagacgttta aactaactgg ctttacggat catgccaaat tcaatgtctc
    15481 accagttctc tatacaacca tcaatgctta ccaagaaatc cgcttcgaaa aagaagatac
    15541 aagtgaaaat gcacggatta acgcaatagt tgtcagaggt aaaatcagta atttacctga
    15601 agatttagaa cagatcaaaa tttctaaatt catcaatgaa ttacctggat ataatgcgca
    15661 agtattgact tttggattta tgatcggatt tttgatcgtt attgcggcta ttgtgatcgg
    15721 gattttcatt tatgtactga cgatgcaaaa gattaatatc tttggtgtaa tgaaagcaca
    15781 agggatcaca ggaggattta ttgcgcgttc tgtagtggca caaacattca ttttatcatt
    15841 tgttggtatt cttcttggtc tattaggaac agtgggaaca tcccttgttc tacctgatgc
    15901 ggttccattc caaagcaatt ggctgttttt tggcgtcatt agcttgttga tgttagtagt
    15961 agctgtgtta ggcgcactat tttctgttcg gacaatcgta aaaatcgatc cgttgaaggc
    16021 aattggataa gaaggaggaa gaacatgaaa gcaatagaat ttaaagaagt agaaaaaagt
    16081 ttccaagatg gcgaccaagt agttaaagca ttgaaagaaa caaccatgag tatcgataaa
    16141 ggggaatttg ttgcaatcat cggtccttct ggttcaggta aaagtacttt tttgactttg
    16201 gcaggcggat tgcagacgcc tagcagtggc tctgtattta tcaatgatca agaattcagt
    16261 aatcaaaaag aaagcgaacg cgttaaacta cgttttaaag aaattggctt tattttgcaa
    16321 gcttccaatc tggttccttt tctgacagta aaaaagcagt tattattagt agataagatt
    16381 aaacatgaac atcgtcaaaa agaagcaaac gaattatttg agcagcttgg cgtggataag
    16441 ttgatcaaca agtatccaga agaactatct ggtggagaac ggcaaagggt cgcaatcgct
    16501 cgtgcattgt acaatgatcc gtccattatt ctagcagatg aaccaactgc tagtttggat
    16561 tcagaaaagg cacgagaagt agtcgatatc ttggcaaaag aatcaaaaga caaaaataaa
    16621 gcaacgatca tggttactca cgatacacgg ctgattgaca aatgcgatag aatatttatg
    16681 atggaagatg gtgtattgaa agaaaaagaa taaccaaaaa gcaggtggaa aaatggtcca
    16741 tcgatacaac aaagatacac agcaaaaaat tttgaaagaa gcgactgact tgtttatgtc
    16801 caagggatat ctcggcactt caacaagaga aatcgcacaa aaagttggga tcacccagcc
    16861 caatctctat cattatttcg gtgataaaga aaaactatat acggcagttt tagaaaacca
    16921 cttaaaagat gttggaaaag ctttgagaga aatcgttcaa acaagcgaaa cgggatttca
    16981 agtaaccttg acaaaaatgg cgcagttttt gattgagaca catctggtcg atttgtttct
    17041 gatgcttcac gatcttgaaa gcaatctttc aaaggaaaca agagatcatc ttttctttct
    17101 atggaagaaa aattatcgtg aaccttttga agaaatcttt tcagcaaacc aatctgtgct
    17161 aagaaatggg atttcaaaag aaatcgcagc aaggcatttt ttcttagtgt tagcacctta
    17221 catcactcgg tcgaaaagac ctgtggaaga accgctaaat gtggcgcaat tgatcgattt
    17281 atatttgaat ggtgtattag gagaagcgta aagctgttat atagggagaa aatagttagg
    17341 tgaagaagca agatatgacg aagatcgtta tatcttgctt ctttatatag gtagtttttc
    17401 ataaagacaa atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg
    17461 gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga
    17521 aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc
    17581 aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc
    17641 cccttttttg cttacttaag ctgattttaa gttttggccc ttagtctgga tctacgaggt
    17701 gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta
    17761 tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta
    17821 cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa
    17881 tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg
    17941 caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga
    18001 tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa
    18061 agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc
    18121 atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa
    18181 cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt
    18241 tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa
    18301 aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa
    18361 ctggaatcac gacatattat tactatggac aagaggaaac attagcacag acaattgctg
    18421 actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc
    18481 ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg
    18541 atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag
    18601 cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat
    18661 ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt tgaacaccgt ttattttttt
    18721 agtagcttca aagctttttc tgtatttgca aagtgaagtt tggcatattt ttcaagcata
    18781 ggcatgccgc ctttctccaa tagttttgcc gctacttggt cagatttcgt tccagctcct
    18841 gatggaaggg tgattccagc ttcagctgat gctttgtgta actcttccaa aaagctggca
    18901 cgactgatga tagaagcagc agcaacagct aaatgatatt gttctccttt agtaacgaag
    18961 tacagttttt cttgtaattg atttttttct aaacgcgcat attttttaaa atttgcttcc
    19021 ggcgtaaact gatcgatcaa aatcgcttct ggtttttctg gctcgatttt ttttagcagc
    19081 aaatggatcg cttgattgtg caaagcgact ttcatccgaa cggcattgta ttttggttgg
    19141 atctcattgt atttttctgg agataagaca agcagtttat aggggatggt tttttttaag
    19201 atgtcagcaa gctggatgat ttggctatct tttaactctt ttgaatctct aacaccaagt
    19261 ttacgtaatt ctgcaagctg atcacgtttc acataagcag cacatactgt tagcggaccg
    19321 aaatagctgc catttccgac ctcatcacta cctaaaacgg ataattgact gatatttttt
    19381 ggcaaagaag aggctgtttt tgctggcttg gcttgtttat cctgtgcctc cccccattta
    19441 tcagcttcta ttgctgcatt cgtaccttga aacatcactt ttccggatgt ataagcggta
    19501 atggtcgtcc cgtctttttt tgcagcaaag ctagtataag gaatctgttt atttaacaaa
    19561 taagagcgat agtatgcttt catttgacta agttggtcgt ttgttgcttt gataatgacg
    19621 ttgctcataa aattctcctc gtttttatac ataaaactat accgcaactc acgacttttc
    19681 gttttctaag agattgcgag acatagcatt tttttaaagt ttatcatata atagttaagt
    19741 atgtgaaaaa tatttgtgga gaacggtgaa agaaccaact tcatggtaca ctaaataaaa
    19801 ctttcatcaa ataggaggaa atgattcgta tggcgcacga aaaaacacgc tacaaagcgg
    19861 tgattgccaa ccaaacatat actattatcg gacgggaaac caagcatcat atggatatcg
    19921 taacgaagct gatcaatgaa cagttagcag aattgaaaca attatcacca caaatggata
    19981 atgaacaagc agcaatcttg atggctgtca atgcgctttc ggatcaattg aagaaacaag
    20041 aaagaatctt ggagctggaa gaagaaacag cagaattaaa gaaaaaaatg atcaaattta
    20101 cagagcttga aaatcgtgtg aaacgcattg aagcaatcga gaacgaagct cgggaagtat
    20161 tgaaagaaaa tggtcaagca gatcatacca ttaaaaatca tgtggaagca caacagattt
    20221 taaacgagaa acgaaaagga caaattaaac aaaaatcttc taattaagat ccttttatca
    20281 aaataagaaa aatttaaaaa aagttagaag agaaaggaac gtagcatgct gagtttgctg
    20341 attttatttt tattgctaat tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa
    20401 atcatattcg cgattggata tgtattgtca tttattgctg cccagcattt ttacaaacca
    20461 ttagcaagtc atttggaatt atacattcca tacccagctg tcacgccaac atcaaagtta
    20521 gcattttttg atcaggtatt tgcctttcac ctagatgaag ctttttatgc aggaactgct
    20581 tttctgatta ttttgctaat tggctggctg cttacccgat ttgtgggggt ttttgtccat
    20641 ggattgacgt atgtgccgat cttgagacag gtagactgga ttgcaggagg catcctgtca
    20701 ctaatcatgg cttacgttac tatatttttg attttgcagt tacttgcctt tgttccatta
    20761 gatgtggtcc aaaatcaatt cggcgagcat agtttcgctc gctttatcgt tgagagaacg
    20821 ccatttctta ccaacaagat gtatgagctt tgggtaacga atattttgaa ttaaggaaaa
    20881 tagtgctgga aaatatttcg ttttttgtac agagtagatt atttaagaag gctgcgtagc
    20941 gtatgtccgg cacagctctt ttttcatagt gaaagataag gtgataaaat gaataaccga
    21001 atccttgaaa cattggaatt tgaaaaagtc aaacagatgg tcagacaatt tgtagtgact
    21061 gcacaaggaa aagaagaact ggcagaattg gtaccagtga gcgaggaaca aacgatcaca
    21121 aactggcttc aagaaactga ggatggattg aaagtgcaac gtctgcgagg cgggatccct
    21181 atacctaaac ttgaaaatat ccgtccccat atgaaacgga ttgagatcgg agcagacttg
    21241 aatggagtgg agcttgcaca agtttccaga gtattgtcta cgacaagcga attgaatcgt
    21301 ttcattgatg atctttatga cagtgaaatc gaatttgcac gtttgtatat gtgggcagat
    21361 cagttagtca cgatccctgt tttgagccgc cgtttaaagg aagcaatcga tgaagacgga
    21421 cgcgtgactg atgacgcttc tcctgagtta aagagcataa gacagaacat tcggcgaagt
    21481 gaacaagctg ttcgtgaaca gttagatggg atcgtccgtg ggaaaaatgc aaagtattta
    21541 agtgatgcga tcatcaccat gcgaaatgac cgctacgtta tcccagtcaa acaagaatac
    21601 cgcggtgtat ttggcggtgt agtccacgat caaagtgctt caggacagac tttatttatt
    21661 gaaccaaaac aagtggtgga tttgaataat cgtttgcgtc aataccaaat cgctgaacga
    21721 aatgagattc aacgtatttt aagtgaactt tctgctgaac ttgttcccca tcgtcaagaa
    21781 atcatccaca atgcttatgt gattggtaaa atggatctga tgaatgcaaa agcacgattt
    21841 ggtaaagaag taaaagctat cgtgccggga atcagtgaag acaatcatgt cgtcttgaaa
    21901 caagcaagac atccactgat cgatcaggaa aaagtagttt ctaatgacat cacaatcgga
    21961 aaagactatc aagccatcgt cattacagga cctaacacag gtgggaaaac gatcacgttg
    22021 aagactttag gtctgttaca actgatgggg caagcaggat taccgattcc tgcaggtgaa
    22081 gaaagtcaaa tcggtatttt tgaggaagta tttgcggata taggagacga acagtcgatt
    22141 gagcaaagtc tatcgacctt ttcttctcat atgacgaaca ctgtagatat tttatcaaaa
    22201 gtcaatgaaa aaagtctggt attatttgac gagctcggtg caggaacaga tccgcaagaa
    22261 ggtgctgctc tagcaatcgc aatcttagat gatcttggta aaaagtctgc ttatgtgatg
    22321 gcaacaacgc attatcctga attgaaagtt tatggatata atcgggcaaa tacgattaat
    22381 gccagcatgg agtttgatgt ggatacatta agtccaacgt atcgtttgct gattggcgtt
    22441 cctggacgaa gcaatgcgtt tgaaatctct agccgattag gtcttgatac agaagttatt
    22501 gatgaagcga agcagttgat gaatgatgag agccaagatt taaacgaaat gatcacagat
    22561 ttagagaatc gccggaaaat ggcagagacc gaatatctgg aaatgcgtca ttttgtttct
    22621 gaggcacaag aacttcacga tgatttgaaa gaagcttaca gctatttctt tgaagaacgt
    22681 gaaaaagaaa tggaaaaagc aaagaaaaaa gcaaatgaag tcgtgtcaga ggcggaagaa
    22741 aaagcagaaa aaatcattgc tgatattcgt aaaatgcaac agcagattgg gcaaggaaat
    22801 gtgaaagagc atcagttgat cgatgcaaag acccaattag caaatctaca tcaagaagaa
    22861 actttgaaga aaaataaagt attgaagaag gcgaaagaac aaaaaacgtt gaaaccggga
    22921 gacgaagtgt tggttacgac ttacggccaa agagggacgc ttttacgtaa aaacgggaat
    22981 cagtggcaag tagaaatcgg tatattaaaa atgaatgtgt ccgaagacga gctgacgcct
    23041 gttgctcctc aaaaagagcc gacacaacga gttatccatg ctgtacgatc tgaatctagt
    23101 agtcacgtgc cgaatcaatt ggaccttcga ggaaaacgat acgaagaagc gctaagtgag
    23161 gtagatcaat atttggattc tgccattcta gctggttatc ctcaagtgac tattgtccac
    23221 ggaaaaggaa caggcgcact tcgcaaggga attactgatt atcttaaaaa ccatcggagc
    23281 gtcaaaagct ttgagtttgc accggcaaac caaggcggaa atggtgcgac aattgttaaa
    23341 tttaagtaaa gcttactaaa ggtaagcaga tggttcgcta tccaagtata gaaatgctat
    23401 actagtcata gattttaaaa atggaggtct ttgatatgtc acaagtaatt acagataaga
    23461 catttaatga agaaacagat aaaggacttg tattgattga tttttgggca acttggtgtg
    23521 gtccatgtcg tatgcaagca ccgattttgg atcagttgga acaagaatat gatgaagaag
    23581 aattcagaat tgctaaaatg gatgtggatg aaaatccaga aactcctcaa caattcggta
    23641 tcatgagcat tccaacatta atgttgaaaa aagatggtca agtagtagaa aaagctgttg
    23701 gtgttcattc aaaagaacaa ctacgtcaaa tgatcgatca atatttataa aaacttacag
    23761 caaaaataag ctgagggtgg gactgaatcc agttccgtcc tcggtttttt tgcggttgag
    23821 ttaaggttat tcaaattaat attttttgag tataattacg ttgtcaggct ggattgtttt
    23881 tttgacagac atgctataat aactttaatt gaatatgaag gggagtacca gatgaacgaa
    23941 cgaatcaaaa acaaattagc tttacttcct gatcagcccg gctgttactt gatgaaagat
    24001 aaaaatggaa cgatcattta tgtcggcaaa gccaaaatat taaaaaatcg tgtccgctca
    24061 tattttactg gtagccataa tacgaaaacg gaacggcttg ttagtgaaat cgaagacttt
    24121 gaatacatcg ttacagaatc gaatatcgaa gcattgctct tagagatcaa tctgataaag
    24181 aaaaatgatc ctaaatacaa cattatgtta aaggatgata aaacctatcc tttccttaag
    24241 ataacgaatg aaaaatatcc ccgtttggtg attactcgta aggtactaaa agacaaagca
    24301 ctgtattttg gaccatatcc cgatgtcggt gcggcgaatg agacaaagaa gatcctcgat
    24361 cgattatttc ctctgagaaa atgcaaacct agtcagacga aggagccttg tctttactat
    24421 catttgggac aatgtctgtg tccttattat ttcgacgtag atccgaaagt ctatacgaaa
    24481 atcgttgatg aagtgaaaaa ctttttaaac ggaggacacg aaaaaatcga gtcagagatc
    24541 catcagaaaa tggaaaaagc tgctgaaaac atggagtttg aaaaagctgc agaataccga
    24601 gaccagatcc gtgccattga gacaatcatg actcgccaaa aaatgacaaa tactgattta
    24661 ctggatcgag atgtatttgg ttatgcgatt gataaaggat ggatgtgtgt ccaagtgttt
    24721 ttcgtaagac aaggaaagct aattgaacga gatgtttcaa tgtttccttt ctataacgaa
    24781 gcagaagaag atttcttgac ttacctcggt cagttttatc aggaaaatga gcatttcatt
    24841 cctaaagaag tcttgattcc tgatacgatc gacaagccca gtgtagaagc tttgttagct
    24901 acgaaagtgc tgcaaccaaa acgaggagaa aagaaaaaat tagttgaatt ggcgagtaag
    24961 aatgcctcag ttgctttgaa tgaacggttt gatctgattg ccagaaaaca ggaacggacg
    25021 atcggagcag ttgaacgttt aggaaacgcg atgaatatcc cgaccccaat acgtatcgag
    25081 tcttttgata actctaatat catggggacg aatcctgtgg ctgcgatggt cgtattcatt
    25141 gatggaaaac cggcaagaaa agaatatcgg aaatataaaa taaaaacagt caacggaccg
    25201 gatgattatg catcaatgag agaagtcatc tatcgcagat attcgcgagt gattcgcgaa
    25261 gggttgcctt tacctgattt gatcttgatc gatggaggaa aaggacaagt agatgtggca
    25321 aaagatgtct tagaaaatca attaggaatc gatattccta ttgctggcat ggcaaaaaac
    25381 gataaacata aaacaaatga gttgctattc ggtacagatt tggctgtagt accgcttgag
    25441 cgaaattcac cagagttttt cttactccag cgaatccaag atgaagtcca tcgattcgca
    25501 atcactttcc atcggcagct acgcagtaaa aacagttttg cttcccgatt agatgaaatt
    25561 gaagggttag gaccaaaacg aaaaaaaatg ctgcttaaag agttcaaatc gttgaaaaat
    25621 atcacagcag ccacgacaga agaattacaa gaaatcggac tgccgaaaaa tgtcgcacaa
    25681 aacgtatttg ataaattaca tcaggaatga ggaaagggca ctttacaaag aaagtaaagt
    25741 gtcctatcat tttatgtaca acaaaaagag cgtttacgtt ttgttggtaa ataataaatc
    25801 gcttacgtct ggtgtctaag gatatatgtg atttcttgaa gaaggagtga agaaatgtta
    25861 gaagtcaagg atcttgttaa aacttttggg aactacacag ctgtcgatca tgtctctttc
    25921 caaatccccg atggaaaaat catggggcta attggccaaa acggggctgg gaagacaacg
    25981 acttttcgtt tgatcttgga ttttttgaca caagaccaag gagaagtgtt atggaatggc
    26041 catcatcttg gggagaaaga ttatgatgtc atcggttatc ttccggagga acggggactt
    26101 tatccgaaag taacgattca agaccaactg ctttattttg cagaacttag agggaaatca
    26161 agaaaagaga ttgaaccaaa aatcgacttt tggatggaaa aatttcaggt aaaagggaaa
    26221 aagacggata aagtaaaatc tttgtctaaa ggaaatcagc aaaaagtcca gctgatcgcc
    26281 acgttaatcc atgaacctaa attgatcatt ttggatgagc cattcagcgg gttagatccg
    26341 gtaaatgctg aattgctaaa agatggaata ttggaattga aaaaacatgg ttcatgtgtc
    26401 attttttcta gccacaatat ggataatgta gaaaaaatct gcgatcattt gatcatgttg
    26461 cgaaacggaa aaatggtttt gaatggaaaa gtacatgaga ttcgtgaatc atttggccgt
    26521 acgaagttat tcttggaatc tggtttaagc caacaggaag tagaagaaat cgaaggcgtg
    26581 aaaaaagtga tccttcatga agacaatacg ttagagatta ccttagaaga tcctgaagct
    26641 gggaaagaaa tctttactcg tgcgactcag ttcggctaca ttcctatgtt taaccagcaa
    26701 ccgccaactt tggaagagat tttcaaattg aaagcaggtg aaatcgatgc gtaagttttg
    26761 gattattacc aaagatgtct atttaaaaaa tgttaagtcg atttctttct tgatcatgat
    26821 attagtccct tttatcatgc tgggtatcat ttatgtagca ggaaactttg ctcaacaaaa
    26881 tagtgatgtc aataaaatcg gcgtattggc tgataatgag caaatcagtc agcaaatgag
    26941 ccaagtcaaa acagatgatt ttgagttcaa agcagttgat tctgaagaaa aagcacaaaa
    27001 agagctttcg gaagaaaaaa tcgatgcgta tttagtagtc tcaactgaga atgataagat
    27061 aaatgggatg ctttatagcg aaaattcatt aggacaaagt acacaactac tgatacaaca
    27121 acaactgact ggcttgcaat cgatgcttcg agcgagtcaa ttaggcattt caccagaaga
    27181 agtagctagc ttgagccaac aagcaagttt gactcgacaa aaagtcaatt ttgattcaaa
    27241 aggaaaaatg acagtcggag aagataactc gacagtccaa tacgtcgtaa gttatgtagc
    27301 aacgattgtc ttgttcatta ttattttgac gtatgcgcag atcattgcac aagagatcgc
    27361 ttctgaaaaa ggcacgcgga tcatggaagt gatcctgtcc agtacgactg cacaaaagca
    27421 tttttacgga aaattaacag gtgttttact tgttgcatta acgcaaatgg cattatacgg
    27481 tattattttt ggtataggat acagtcaatt caaagatatg gaaatggtca aaaatatatt
    27541 agacggtatc tcattggata acttgattgg accattcttg tggtattctc ttctattcat
    27601 gatctttggc attttgattt tttctgtttt agcagcttta tgtggctctt tggtcaacaa
    27661 agcagaagat acagcaaaag caattttgcc agtgacttat ctatctctag gcggatacat
    27721 gcttggattg atactaggag cttcagatcc aaacaatatc gttattcgga tcacttcgta
    27781 tattccattc ttatcttcat atattatgcc ggttcgctta gcaaatgaga cagtcggtat
    27841 gggtggagca ttgatttcct tgttggtact tgtagccgta acattcttat tgatgatctt
    27901 gtctgcaaac atgtacaaat cgaatgtact cgtttatagc gaaggcggat tatggagttc
    27961 attgaaacaa tcgatttcaa ttatgaggaa tgaacgaaaa aaagcataaa acaatcctaa
    28021 aatacagaag gaagctggac atctattgtt cccagcttct ttttgtatag acctatttta
    28081 tttattttac tatattggat atatattcat tgaaaatagt gtataacaac tttataatat
    28141 tgagtatacc aatttttgga ggcgttttaa tggaccgtta tttagattta ctttcagaaa
    28201 aattcccctc aacggaggat gtcatcacgg aaatcatcaa tctcgaagca attttgcagc
    28261 ttccaaaagg aacagagtta tttttgagtg atatacatgg cgaatttcct gcttttgatc
    28321 atattctgcg tatcggttca ggaaatctta aagaaaagat caaagacctt ttttctgaac
    28381 aaatgacgga aacggatatc agccaattca ctatttttac ggcttatcca gaatatgcat
    28441 taacgactga ttggtacaaa gaacaagata aatcgcagct gatccatcat ttgatcgatc
    28501 ttttacggtt tacgacagtc aaatataccc ggtcaaaagt tcgaaaagga ttgccgaaag
    28561 aatatagcta tatcattgag gaactcttgt atatcgatga ccgtatcaat ggaaaaaaag
    28621 actatgtaaa aaaaatcatt gaacaacttg ttttgatgaa ggaagaagaa aaatttttaa
    28681 cgaaacttgc acagacgatc caaaaatctg tcatcgatca tttgcatatc gttggagata
    28741 tttttgaccg aggaactcaa gcagccaaag tgatggatcg aatcatggat ttttcttctg
    28801 tagatatcca atggggaaat catgatattt tgtggattgg tgcgtattgc ggctctgagg
    28861 catgtttact gacactttta cggattgcag cacgctataa ttatttattt gaattagaga
    28921 aagaatatgg gttgaattta cgtccattgt tttcttttgc tcatcaaact tatcagaaga
    28981 atccagtatt tgcgcctaaa aacagtaaaa atctatccga aagagagcaa gaagaattag
    29041 aaaaaatcca tcaggcactt tctatccttc aattcaaatc agaacatcag ttattggatc
    29101 gtcgacctga atttaaaatg gacgatcgta aattgctgga aaaaatcgac tatgaagcat
    29161 caacgattga tttagaaggt cagctctatg agttgaaagg aacttgtttt caaacgatcc
    29221 agccagatga tccaaaaaaa ttcttggaag aggaacaaaa agtgattgat agtttgatgt
    29281 attcattcca gcactctttg agaatgcaaa aacatatgac gtttttgatc gaaaaagggg
    29341 gaatgtatct cacgaacaat cagcatgtgc tgtttcatgg ctgtatacct gtagacgaga
    29401 agggacagct cttagcattt gaattagacc aatcttatcg cggaaaagaa ctgctgggct
    29461 tttttgaaaa acagataact gaaagcgcaa aagatctgac tgcaaaagaa gatttagcaa
    29521 ccgatttgat ttggtatgcg tggagtgggc cattttcgcc tttattcggc aagagtaaaa
    29581 tggcgacttt tgaacgttat tttctcacag agcctcatac gcacgtcgag aaaagcaatc
    29641 cttattatca cttgcgagat gaagagtggt tcagtgaaaa aattttagca gaattcgaca
    29701 caaaagaaga agggtcagca atcattaatg ggcacacgcc agtaaaagta aaaaaagggg
    29761 agtcaccgat caaagcaaat ggaaaagtat ttgtcattga cggaggttta tccaaagctt
    29821 atcaaaaaac gacaggaatt gccggttatt cactattatg caattcttat ggtttccaaa
    29881 tcgtgacaca ttatccgttt ttgcctattg aacaacaatt tgcaaaaaaa tccgaccaaa
    29941 caaatgtgaa aaaagtaatc gaacagcaac taccacgcaa actcaatcgt gatacaacaa
    30001 aaggtatgga actgcagcag cagattaccc agctcaaaag gctgttggac tatcgaaagg
    30061 acgattgaga agaattccag tgaacattgt tccatagact ggagactgag acaaaagtcg
    30121 tgtcgcagtc tctttttccg gataaacggt gttcttctgc caactcctcg gtcacaagtc
    30181 acactattca acaaacatga aaagctgttt tttgaaattg ctccttgtga ctcagagttg
    30241 aacggcaggc tcacgacctc ttggtataaa cggtgttcaa aagccagttt ctcggtatta
    30301 agccaaattt atgcaaaaat gtgaaaagcc attttcacaa aatttttctt aatactcgaa
    30361 actaaacagc tttttcacaa ccccctcaga aagtctatta aaattcattc acaaagaaat
    30421 cgaataagcg ctgttcagat ggatgtacac ggtgggtaag ttccggatgc cattgaacac
    30481 cgaggatttt atgcatatca tctttgcttt gaaccgcttc aatgagtcca tcgttagaaa
    30541 aagcgatggc ttttaataaa ggtgaaagat ctttgactgc ctgatgatgg taagaattca
    30601 caaaatatgg atcggggaga aggttgctta acaaactgtc tgaaacaatc ttgacttcgt
    30661 gagtcgtaaa ttgcggggca gtgggctgtt gttcatgttt tactgaccac tttggatata
    30721 atgaaagatc ttgataaagt gtaccttcca aaacgacatt caatagttgg agtccccggc
    30781 agactgcaaa aattggtttt ctttgtttca atgcttccaa gatcagtgcc tgttcaaatt
    30841 ggtctcgttg aatatttgtt tccgttagct ttggatgagg atcttccatg taaagttgtg
    30901 gactgacatc ttgaccacct gcaagaataa gtttgtcgat ttgtccaatc agttgaggag
    30961 ctgaatctgg agacatgact ggcagaataa gaggcaatcc ttgagcatct aaaactgcat
    31021 ccacgaatcc ttgcggtgta tagctcactt gatttccttg gaaagtatct gttgctcgaa
    31081 tcagttgatt tccggcaatg ccgatgattg gtttcatttg tattccccct aaagtagact
    31141 tattgtaaca aggatttaaa aaaaattaaa ctatgtaaga cttgccccta tacttatctg
    31201 ttcttgctgt gttactatct gtaaagagaa atttcgtgtg aaattcatga cgatagttga
    31261 aaatattgct taatttgtta taataaaaag taagcagaaa taaaaaggtt gggcgtaagc
    31321 ttcaaggatc tagttcttca aggggtgaga aggacatttt attgaagggg cctgcctata
    31381 ctaagttatc tttagaggta catactttca atgaaaaaag aaagcatgta cctctttttt
    31441 aaatcgaaag gaagtgaagc aagtgttaga agctttggaa caaatccgaa aagcagaaga
    31501 agaaaatgaa aagcgtgctc aaacactgcg gcagcagctg aagatctatg caacagaaaa
    31561 agagcaggaa ttggaaacga tccaaacaaa ctatcgacaa caactaaaac agttggtgga
    31621 agaaaaagaa caatttgaaa tacaagaatt agaaaaagaa aaaatgaaat tgaaagcaga
    31681 agcagacaag gtgcaagcaa ctttgaaaga tcaatatcaa aagcatcaga gtcaagcgat
    31741 cggtgcgatc attgaaaggg tgaaagaaac ttatggccgt cactaagatg gaaaaagtga
    31801 ccctcatttc agataaaaga aatcaggaga ggattttaca agcagtacaa ggaatccaaa
    31861 acgttgaaat ccgggatctg tatcaaaata gaacaaacaa ccagtgggtg gagaagtatt
    31921 ttccagatac cacgattatt gataaagaag caaaattaag cgctttaggt aatcggttga
    31981 aagacatcca agaagctatc caattcatac aacaccacgg cgatagccgt caaaagaaac
    32041 tccatttgaa acgtcaagaa ctttccttac aagatttgga agccaattat tccgaagaaa
    32101 atttcataaa taaattgaaa gaagtccttg atttaaaaga aaagtgggaa caactaactg
    32161 aagaacgtga tcgtttagaa gaagaggaag aatggcttct tcattggcaa cagctggata
    32221 ttgctccaag tatgtatgca agtaagacga ccatttttga gatggggact gtgagtgcag
    32281 caaattttga aagtttcaag gaagaactga gtaagcttga tgacgtttat atggaagaaa
    32341 tagattatac gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa tcagttgctg
    32401 ttatagaaga aattgctggg aaatacgggt tcaataaaga aacgtatcct tatgaacaaa
    32461 atcctaaaga aaaactgcaa gagaccaagc agcaattatc cattgttcac gagcagcaaa
    32521 agaagataac gacagcgtta ggtcagtgca gtggctatat ccaagaattt gaatgggtag
    32581 aagaagtcac acttgccgtt gcagaacgag aaaaaataaa agaacggttt atccatgcga
    32641 cttatctgat cgttttgcaa ggttgggtag atgcagaaga aaaacagaat ttgctgcatg
    32701 tgttaaacga aaccgtatca gaagaagata tatacgtcag tttcgaaaca ccaaactcag
    32761 aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttgtc gaaccttttg
    32821 aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca acaccttgga
    32881 tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt tatggtttgc
    32941 tgatgctcat tgggacaata ctagctcaaa aactacttgt tctaccacgc gggatgaaac
    33001 gtttcgtaaa attctttgag attctctcga taccatcgat catctgggga ttgatctaca
    33061 gttcattttt cggacagtca ttaccaaaag aactgtttgg catccgcttg cctttcccaa
    33121 tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt ttcggtctga
    33181 tacagatttt ggttggatta tttgtggcag caaaggaaaa tatcaggaga aaagattact
    33241 tgagtgctgt cagtgatgga tttgcctggc aagggatcct aattgggatc gttattgcgt
    33301 tggttggtgc catgttgata aaaaatacag catttgttta ccttggcgct gggctatcga
    33361 tcattgctgc cttatgtatc ttgatcgttc cgattatcca aacgccgtct aaagtcaaag
    33421 gtgctgccaa aggtgcgtat aatctttacg gactgaccgg ctatatcggt gatttagtca
    33481 gttatacacg tttgatggca ttgggtattt caggcggaag tattggagcg gcattcaata
    33541 tgctagttgc tttcatgccg ccagctgctc gattcagtgt agggatcctt ttgatcatcg
    33601 ctttacacgc actgaatatg tttttgacac tgctaagtgc ctatgtccat ggcgcacgat
    33661 tgcagtatgt cgaatttttt gggaagtttt atacaggagg cggacgtgcc tttgatccgc
    33721 tgaaaacggc ggaaaaatac gtcaacatca atcacaagaa aaaaaacaat aaataattgg
    33781 aggattttta tataatgatg gattacttaa ttagtcaaaa tggtggaatg gtatttgcgg
    33841 tattagcgat ggcaacagcg acgatttttt cagggatcgg ttcagctaaa ggtgttggaa
    33901 tgacaggtga ggcagcagca gcattgacaa caagtcaacc cgaaaaattc gggcaggctc
    33961 tgattctgca attgcttcca ggtacgcaag gtttgtatgg attcgttatt gcctttttga
    34021 tttttatcaa cttaggaaat gacatgtcag ttgtacaagg actgaacttt ctaggtgctt
    34081 ctctgccgat tgcttttaca ggcttgtttt caggtattgc ccaaggaaaa gtcgcagcgg
    34141 caggtattca aattttagcg aaaaaacctg aacatgctac aaaaggaatc atttttgctg
    34201 cgatggttga aacatacgcg attttaggtt tcgttatttc cttcctatta gtgttgaatg
    34261 cctaagcata aaggaggaaa agcagatgaa cgccattgaa aaaatcatct ctcagatgaa
    34321 cgaagcagca gagcaggaaa gagcggcgtt agaacaagaa gagcgaatga agatcgatca
    34381 aaactttgaa caaaaacgta cgcaagttga aacagaacat caaaaacaaa aagaaaaaca
    34441 aatcgaatta ctcgaaaaaa aataccgtca gctaaggaat cgccagcaag tagaagtacg
    34501 acaagaaaat ttgaatgcga agcaggaatt tttaagacga ttgtttgcag atgcggtaac
    34561 tgaaatggaa aattgggatg aatctgaaca aatccagttt atcaaaaatg cattgtattc
    34621 attacctttg acaggaaaag tagcattcat tgctggagaa aaatcagcag cctatctttc
    34681 tcagacgttg ttggatgaat ggaacaatga acttcccttt atgatggttt taagtgatga
    34741 aactgttgct gatcaagcgg gttttctgat aaacgatcaa ggagttcagt ataattttct
    34801 tttcagcagc cttgtacaag atatccaagg aacgatgagt tttgagattg ccaatcaatt
    34861 attcgaataa ggagggatca gtgtatggaa taccacgaac taaatccctt gattcgcgga
    34921 agagagcttg aacttttgtc caatgagaca tttgaacgga tgattcaaac aaattctgtc
    34981 gaagcacttg gtgaaatatt gaaatccacg atttacagtc catatattta tgacggatat
    35041 gaatatgact tcgaacagaa tcttttaaag gagcagagcc gtctatttcg ctggttaaag
    35101 gaatcagcac cggagcctga aatcgtttgg atctatacga tgcgttatac tttccataat
    35161 ctgaaggttt tgacaaaagc tgagatgaca aatcaaaatc tggaccattt gtatatcgac
    35221 gatggatttt attcattaga aaccttgaag gatgccattc atacgcaagt ttcaaatgaa
    35281 cttcctgaaa gcattatgga cagtatacga gaagtacgtg attattttga agagtctacc
    35341 attcttcagg gaattgacgt gatttatgat cgacagtttt tgacagagca gcgacggctt
    35401 gccgataagc taggatatca agaattgctg acagaagtca ttgcttttat tgacttgacc
    35461 aatatcacaa cgatggctcg aggaatattg cagaaaagaa catatgggtt catgacgacg
    35521 gtactttcca gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt agataaagat
    35581 atggaagatt acacgaattt tctattagag acagattatg ctgattttct gcgccctgtc
    35641 atccgtgagg gaaatattga tctcgtaaag cttgaacagt tgaaagatga ttatctaagt
    35701 tcattgtatc aagaagcaca aacccaggct ttcggaccac tgccactact agcctttttg
    35761 aacgcaaaag aggtagaaag caaaaacctt cgtttactgg ttatcggcaa aagaagccat
    35821 ttcgaatcag aaacaatcag aaagcgggtg agacaagtct atgacgcata agatcggtgt
    35881 agttggcgac aaagattctg ttttgccatt caaattgttt ggctttgatg ttcgttatgg
    35941 tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg agtatggcgt
    36001 gatttatgtc acagagcaat gtgcagcatt gagcccagaa acgatcgaac gttacaagga
    36061 acaaatgata cctgctatcg tattgattcc caatcatcaa ggatcattag ggatagggct
    36121 ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat agtatgaaaa
    36181 ggagatagaa attttgcaaa ttggaaaaat cataaaagtc tccggacctc ttgtaatggc
    36241 tgagaacatg tctgatgcca gtatccaaga catgtgtcta gtgggagatc taggagttat
    36301 cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt caagtatatg aagagacttc
    36361 tggaattggt ccaggcgaac cggttcgctc tactggtgaa gctttatctg ttgaattagg
    36421 accagggatc atttcacaaa tgtttgacgg aatccaacgg ccattagata cctttatgga
    36481 aatcacacaa agtaatttct tagggcgcgg agttcaattg cccgcattgg accatgagaa
    36541 aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg gagatgttat
    36601 cgggatcgta gaagaaacaa aagttataaa acataaaatc atggtgccaa atgggataaa
    36661 aggaacgatc aaaacaatcg aaaaaggttc attcactatt gatgaaacag tatgtgtcat
    36721 tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa aaatggccag taagacgtag
    36781 tcggccaatc aaagaaaaat taaacccgga tgcacccatg atcactggac agcgagtgat
    36841 tgatacattc ttcccagtga caaaaggagg agcagcagct gtacctggtc cttttggtgc
    36901 agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca tggttgtcta
    36961 tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat tccctgaatt
    37021 gattgaccct aatacaggag aatcgttgat ggaacgtacg gtattgattg ctaatacatc
    37081 aaacatgcca gtagcagctc gagaagcttc tatctatacg ggaatcacaa ttgcggaata
    37141 tttccgtgac atgggttatg acgttgctat catggcagac tccacttcac gatgggcaga
    37201 agcacttcgt gagatgagcg gacgtttgga agaaatgcct ggtgatgaag gttatccagc
    37261 ttatcttggt tcacggcttg cggaatacta tgaacgatct ggaagagtca ttgcattagg
    37321 atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct gtatccccgt ctggagggga
    37381 tatttcggaa ccagtcacac aaaatacatt gcgggtcgta aaagtattct ggggtcttga
    37441 ttccagttta gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa gctattctct
    37501 ttattcaaca gaagtaggga agtatatgga tcatatcctt caagctgact gggcatccat
    37561 ggtcaccgag ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa tcgtgcgttt
    37621 ggtagggatt gattcccttt cagataatga ccgtctgaca ttagaagtgg caaaatccat
    37681 tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc gatacattca cttcaagaga
    37741 gaaacaattc aaaatgttga agatgatctt gacctttggt gaagaaggac gccgtgcgtt
    37801 gtcgttaggt gcttatttaa gtgagatcat ggacggtacc gtatcgattc gtgaaaggat
    37861 cggacgaagc aaatatatcc cggaaacaga attagaaaaa ctagatcaaa ttattggtga
    37921 aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg attaaagaat
    37981 atcgtacgat cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt gctggtgtca
    38041 aatatgaaga attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt ggacaagtat
    38101 tggaagtcca agaagataaa gcaatggtcc aaatattcga aggaacaagt ggtatcaatt
    38161 tacgtgactc ttctgtacga tttttaggcc atcctttaga actaggtgta tcggaagaca
    38221 tgatcggtcg agtattcgat ggtttgggac gaccgaaaga taacggacca gagatcctgc
    38281 ctgaaaagca tttggatatt aacggagaag taatcaatcc agtagctcgg gattatccag
    38341 atgagtttat ccaaacaggt atctctgcaa tcgatcattt gaatacatta gttcgtgggc
    38401 aaaaattacc agtattttct ggctctggtc ttcctcataa agaattagct gctcagatcg
    38461 cgcgacaagc aacggtactc gattcatcag atgatttcgc cgtagtcttt gctgctattg
    38521 gaatcacttt tgaagaagct gaatttttca tggaagattt tcgtcaaacg ggcgcaatcg
    38581 accgttctgt catgtttatg aatttagcaa acgaccctgc aatcgaacgt atcgctactc
    38641 ctcgtatggc tttgactgct gcagaatatc ttgcttatga aaagggaatg catgttttgg
    38701 ttatcatgac agacatgacc aactatgcag aggcattgcg agagatttct gcagcacggc
    38761 gagaagttcc aggaagaaga ggatatcctg ggtatcttta cacgaactta gctactttat
    38821 tcgaacgagc aggaagaatc cgcggactaa aaggatcggt tactcaaatt ccgattctaa
    38881 caatgccaga agacgataaa actcatccaa tccctgactt gactggttat attacagaag
    38941 gacagatcat cttgactaga gagctttaca agagtgggat tcagccacca attgatgtac
    39001 tcccgtctct ttcgcggtta aaagataagg gaactggacc cggaaagaca agagaagacc
    39061 atgcagctac gatgaatcag ctttttgcag cctatgcaca aggaaaacag gcaaaagaat
    39121 tagccgtagt tttaggtgaa tctgctttat ctgatgtaga caagatctat gcgaagtttg
    39181 ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt tcgattactg
    39241 aaacacttga tctagggtgg gaactgctat cgatgttgcc aagaaccgaa ttaaaacgaa
    39301 tcaaagacga catgctggac aaatatctgc cagaagggaa gtgagttctg atggcacgat
    39361 tgaatgtgaa tccgacccgc atggaattga ctcgattgaa aaaacagttg acaactgcca
    39421 caagagggca taaattatta aaagataagc aagatgaact gatgcgtcaa ttcatcctgt
    39481 tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca gaagcaatgt
    39541 ctgattttgt attggcaaat gcttcgatca cggaaccttt catcgaagaa ttatttgttt
    39601 tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt gtaaaagtac
    39661 ctgtgatgaa ttttgaatat gatgaacagc tgacagacac acctttggaa tacgggtatt
    39721 tgaattccag tgccgaactg gatcggtcaa tcgatcggtt tactaccctg cttccaaaac
    39781 ttttggaact gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa atcgaaaaga
    39841 cacgtcggcg agtcaatgca ttagaatata tgacgatccc tcaattagaa gaaacgatct
    39901 attatattcg tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg ttgatcaaag
    39961 taaaaaatat gggtaccact gactaagtgg tatctttttt taaaagagga aaacgatgat
    40021 taaacaaagc aaacaaataa agaaacgttt gccggctgtt cagcttctcg cattaggatt
    40081 ttttctcctg attttagttg gcggcggcct acttacccta ccatttttta gtaatagtgg
    40141 gcatgggaca aattttatcg atgcgctttt cactgcaact tctgctgtct gtgtaacggg
    40201 attgacaaca ttgaatacag cagagcattg gaacagtgct ggtcaatttt tgatcatgct
    40261 tttgattgag atcggcggtc ttggttttat gatggttccg attctatgct ttgcattgat
    40321 aaagaagaaa gtcagtctca gcacacgtat tatattaaaa gaagcgctga atctagaaga
    40381 agtatcaggt gtgatgaagc tcatgcttta catcctaaaa ttggcctttg tgatccaatt
    40441 gattggtacg gttgctttat cttttgtatt tgtgcctgaa tttggctggt tgaaaggtat
    40501 ctggtacagt ttgttccatg caatctctag tttttgtaat gcagggtttg atttgttagg
    40561 agacagttta gccaatcacc agcaaaatgt ttatttgatc atggtggtat ctgctttgat
    40621 catttctggt gggttaggct tcatcgtatg gcgtgattta ctggaatatc atcagaaaaa
    40681 gaagatgacg attcattcaa aaatcgcatt aaccgttaca ggtacccttt tattaggcgg
    40741 attcgttgtg ttctttttca cagagcgcaa tggggcaacg ctagtggaag ggaatatgtt
    40801 tcaacgattg gcaaacacct tttttatgag tgtgacacct agaacggctg gatattattc
    40861 agttgattac ctccatatga gccatgcagg tctgatttta acgatgtttt tgatgtacat
    40921 cggcggaaca tctgggtcga cggccggcgg attgaaaaca actacgttag gtgtgttgct
    40981 gatccagatg cgttcgatgt tgaaaggacg tacgagagcg gaagcatttg gccgtaccat
    41041 ccgacaaggc gcagtgttgc gggcacttac tttatttttt gttacgttga cgttgtgtgt
    41101 agtcgccatc atgattttat cggtgactga aacgatacct aaaacttctg gcatcgaata
    41161 tattgctttt gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg gattgacgcc
    41221 agacttgact gcaataggga aattgatcat tatagcgctt atgtacattg gacgagttgg
    41281 tgtattgact gtggtcttct ctctgatgac aaaagcacaa agaacacaat caaaatacaa
    41341 atatccagaa gaaagtgtca tgattggata atttcatctt ctgacagaaa cctagtgatt
    41401 tagtaaaata agaaaataaa ataatttttc ttgcttcttg gatactttaa gtgcaaaatg
    41461 ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg ggtagtgcct cttttttatt
    41521 gaaaggaatc aataagattt gattccttta tttatcaata ggttataatt catatttttt
    41581 tattttttct tttacgatac ttagtccaga agctaaaatt tcaaggttat tagaggatag
    41641 ggaaattcga acgaatgcat ctggttgcgt acgtgtagta aagcgtctgg aatgataaag
    41701 tcggataccg ttagcaagaa agtcattctc aattatctta gggtcaatct caggatcaac
    41761 tggaagaatt cgatagtaag ggacacgatt cgttggtttt tctactttga aaagagaatc
    41821 aaaaagctga ttggctcttt ttgtaagact taatttagtt tctaagatcc tatttgcttc
    41881 atttgaatgg aggatttgag tgatgacttc cacatctaat ccagaagttt tcacatttat
    41941 attaaaaatt gcttgttcta tcttgctttg gacatgtact ggaactacaa gataagcaac
    42001 tcgcaatcca gaacaaataa attttgtcat tccagctaag tagattgttt ggttaggtaa
    42061 taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa atgaatggat
    42121 atcatcttca ataacccata gaccttcttt ttcaatcact tcggctaatt gctctcgtct
    42181 tttgtgagag atttgaaacc ctataggatt attacaagaa ggcataagaa agacaccatg
    42241 aatcttcttt tttctacatt cctgaatcaa tatatcaggt cgcattcctt gctcgtcaaa
    42301 atcaattgga acgatctcta aatgatataa ctgagccaat tcaatgaaat tagcgtatgt
    42361 atatcggtct gttgctattc tatcacctgg agagaaaagt gcagaaagtg tgatagccaa
    42421 tccattttga acaccggaaa caatagatag agattctaag gacggaatga ctccttgagt
    42481 ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg cctttgggat cttggtaatt
    42541 aagcaaacca acaagatttg ggttattact agttgattga ataaacggga caacgaattt
    42601 attacattct tcaaaagaac taaccagacc aaaatcaatg atatgttcgg ttacttcatt
    42661 ggtagagatg gtgatggact caaagacatt gggagaaata aatgtgccac ttccaatatt
    42721 cgtatacagc aaacctttct caattccata tttataagcc ttgccaacgg tagtaaaatt
    42781 taaatctaaa taatcagcta gctctctttg ggagggtaac tgagtgtttt tctgtaattt
    42841 attctttaaa atatcttgtt ctaattgttg aatcaacgat cgataataag gacgcttcaa
    42901 tttttgtttg tctggtcgcc aagttaacgg gtaattgtca taagaattaa taggcatttt
    42961 ctccactcct taaaattgta taccatacaa ttttaccatt gattgtattg attttagaag
    43021 ttaatctttt gtgaggaggg aattgtctat gaaaattatt aattttgagg aagtagtagc
    43081 ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg attatgaaag
    43141 tggtaaaatc agccaaaatc ctcgattggt ggaaatttta cctgacggaa aaggcaaaaa
    43201 tatgtttgcg atgatgcctg cttatttggg ggagaacaga tattttggca caaaagtcat
    43261 taccgctttt ccggataatc atcagtatca tttaccttct catttaggag agattttctt
    43321 gtttgatgct caaaacggac tacccaaagc attgataaac gcaaatgcag tcacctggat
    43381 tcgaacagct gctgtatctg cgctggcaac agattactta gcaaatccgc atgcagaaac
    43441 acttgcttta attggagctg gtcaacaagc attgtcgcat ttgactgcga tttctaccat
    43501 tcgtcccatc actacagtgt ttgtttacga tattttagaa gaaagacgaa atacttttat
    43561 cgctaaagct aaaaaacagt atcctaaaat aacgtttata aatgcaatat cagtagaaga
    43621 tgcagtacgt ccagcggaaa tcatttgtac gttgacctct agtaaagagg catttcttca
    43681 aaaagagtgg gtaagggaag acgcacatat caatgctgtg ggcacattta ctcctgatac
    43741 aagagagata agtacatcgt tgatggaaac ttctcaaatc tatgtagatg attactcagc
    43801 tgccttaaca gaaagcgggg acctgcttat agccatggca gaaggagatt tagaggaatc
    43861 ttgtattgtt ggtacattag gcgaattagt ttctgagaaa aaaacatttt ataaaggaga
    43921 atcgaggccg acgattttcg acgcagttgg tctagctatt gaagatcttt gttgtgcaga
    43981 atatatttat aacaaattag agaaaggggc tttaaaatga acttagatta ttacgtagtt
    44041 gatgcatttg cagatgaggt gttcaaagga aatccagcag cggtttatgt gctagaagaa
    44101 tggctgccag aaggtacgat gcaaaaaatt gccattgaaa acaatttatc tgaaacagca
    44161 tttacagtga agaaaaacca agaatttgag cttagatggt ttacgccaga tcgagaaatc
    44221 gatctttgtg gtcacgcgac attagcaaca gcatttgttt tatttaacta ttacaaaata
    44281 cctgatgaaa cgattaaatt ttctactcaa agcggtaatc tatttgtgac aagaattaaa
    44341 gattattatt acatggattt tcctagtatt atgccgaaaa aagtgccgat attgtctgaa
    44401 tatgaagaag cgattggggc taagattaaa gaagcctact tagcacgtga tttatttttc
    44461 gtattagaag atgaaaaaac ggtggctaaa ttgcagccag attttacagc tatcaaaaat
    44521 tttgaattag gaataggagt catcgtgaca gcagaaagtg tgcaaaaaga ctttgtttct
    44581 agaaccttct ttccaaaatt aacaatcaat gaagatccag tatgtggatc agcacattct
    44641 aatcttattc cttattgggc agaaagaatg aataaaacaa aattgtcagc ttatcaaatg
    44701 tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag atcgtgtgat aataggagga
    44761 aaagcagttt tgttttctaa aggtacctcg tttatttgat ttctttcaaa tgaattaaaa
    44821 ataaattggt ggagagtggc aaccttatag ctacaatggt tgttaaaaaa agacgcaaac
    44881 tttttcaatt taatgaaaag tttgcgtctc attttaatta ttggtgtttg ttttttattc
    44941 aatcaagagt aaaatgcccc atactaacta tcctcggatg acttcgattt tataaccatc
    45001 agggtcaatg acaaagtagt aagaaggcgc agtgccaggt aatcctttta agtcagtaat
    45061 ctcaagacca gcagctttat gttgctcgtg taatttttct agatcatcag ctgctatagc
    45121 gatatgacca tagccgtctc ctagatcata cgcaccatga tcatagttgt aagtcaactc
    45181 taattcatag tcatccccag gaagtgtcaa ataaacaagt gtaaatttgt tttctggaaa
    45241 atcacggcgg cggctttctt caaaaccaaa tgccttttga taaaattcga ttgatgcttc
    45301 aagatctttc actcgaacac atgtgtgagc cattttcata gattcatctc tccttttcct
    45361 tttactcaat cataacaaaa agaagtaagg aaatagttga tttgtgcttg ttcatgagaa
    45421 atttctcatg taaaatggat agaaaataaa actgttaagg tggaaactat tcatggataa
    45481 accaattttt ggtgaaaaaa agcaatttgc tgattatcaa acccgttatg cagcatatat
    45541 tgtcatagaa agagatcagc agattgcagt aatcgaggct ccaaatggtg ctttttttct
    45601 tcctggcggg gaaattgaag gagaagagac gaaagaagaa gcgattgtcc gtgagctgat
    45661 agaagaaatg ggaattgtgg cagaaatttc ttcttatctg ggagaagctg atgagtattt
    45721 ttactcgaat tatcgtgcaa cttattatta taatccagga tatttctttg ttgctgattc
    45781 ttggaagcgt ataggagaac caactgaaaa gaccaataaa atttggtggg tgagccctca
    45841 agaagcgctt gtgaaattga aacgtggtag ccaccgatgg gcagtcgaga gatggatcga
    45901 aaaaaataaa aactgaaaga aagcgagtag atcagctttc tctcagtttt tttatttatt
    45961 acagtacata ttttttgatt gcttcagcta caccgtgctc gtcgtttgat gaagtgatga
    46021 catttgctgc ttctttgaca agagaaacgg catttcccat tgctactcct agaccagcat
    46081 attcaatcat ggataagtcg ttctcgttat ctccgatagc catgatttca tcttgatcaa
    46141 taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct ttatttagta
    46201 cttcgaagta atagggcgca cttttgaccg ttgtgtattt atcacggaaa gtttgcggta
    46261 aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat catcatcatt ttgacgatgt
    46321 tcatgtctgg tgtcatttct tcaggtgttc gatattttag cggcatcttg actaaacttg
    46381 cttcgtggat cgtataagcg ctgatatctc gattaggcgt gtagatcgct ttgtcatcga
    46441 tcgaatggag atgactgccg acacggcgtg ccatcacttc gatctctagg aaatcttcat
    46501 gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt ttgttgtacg agtgccccgt
    46561 tataagtaat aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac tgttcctgga
    46621 cacctggtaa gggacggcca gtgcataaaa cgatcgaaac accttttgca acagcagcgg
    46681 caatcgcttc ttttacttct tttgttactt ctcttttctc atttaataaa gtaccatcga
    46741 tgtcgatagc gactagtttg atagacataa tttcctccaa tatattttaa tttatttcaa
    46801 tcaatgcgcc attacggata tggctggcaa attcttgata agtttcgtca aataaatcat
    46861 aatgatcacg caaggacgga tcaaccattt cttttggaaa gaaaaatcgc tcatctcctt
    46921 gttcttgtcc cgctaatgca gcaacaagct gactaacctg tgataattct atcaaagttc
    46981 cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg cgataaaaat
    47041 cataaggtag atcgtagcta gaattcacag cagtgtaata aacaggatta tatccgacct
    47101 tttctactaa caaagtgagt tcttggacta attttgaatc ccggttgcca gaaaaagtag
    47161 cagatttcag tggtttacga ttcaaaaaac gtttagctaa atcatttaaa ataggatcag
    47221 cagattcaga ccattgagta aagtaagtgc ccaagacccc atcatcaagt ttgaggtatt
    47281 cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg agttcaaaac
    47341 tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc aaaattactt
    47401 ccataccacg ggaaacagga tggaagtaca cttgcacata catttgatat cggctaacga
    47461 tataatcttc cactgcatgc atgccactca tggaaaaagc gatcccgcct tcataaggac
    47521 gaatcacacg taatattcgg gtaagatcaa atgttccata ttctgttcca gtgaaataag
    47581 catcccgaag caaatagtcc attcgatctg catcgatctg actagaaatc atttggacaa
    47641 cttgtgggtt ggggtaagtt ttttggatca cactagctac cttttcagga aaaccttctt
    47701 ctactcggtt aagaatctga tacacttctg tttctggaga agtgatgatc tctacagtga
    47761 tggcttcatg attcgtatgg aagatatgtt caaatgtatg agagtaagga ccatgaccga
    47821 catcatgcaa cagtgccgca cataaagtca ccagccgttc ctgatcatcc cagcctccgt
    47881 tgcctatttt ttcttttgaa aaatttcgag aaaagatatc gcagattcgt cttgaaatct
    47941 cataaacgcc taatgaatga gtaaaacggc tatgttctgc tccgtgaaaa gtgaaagaag
    48001 aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc tttcgaattg atcaaatcta
    48061 agattacttg atgctggaca tggatatagt tatggactgg atcacgaaaa actttttcca
    48121 taggcagcat ttgatattta taaggggttg tcataatgta gctcctttat gttattacgt
    48181 tgttccattc tctggatttg gttgttccat tgctccagct ggatttcttt ttttagccag
    48241 tcggtagaat cctgtaaatc acttgcagaa tacagctttt caaaagcttt tgcaaatgct
    48301 tctttgactt cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt tgccattgta
    48361 gcaggatcca ctgcaggata gccatttgta ccaaagtctt cttttaaagc agattgataa
    48421 aatcgcttca tgacttgccc gcgtttttcc tgattaccgt tgacacttaa gtacatcata
    48481 acagaaatcc catttttgat ccgacgctga gcgataccgg caaatttttt gccatggatg
    48541 cttaaatcat aagttccagg acaataagaa tgggtgattt cgaatgcttg tatcggtgcc
    48601 tttggaaaag cgtatttagt caataggagc atttgctcgt aagcgtcatc tatggttatt
    48661 ttttttgtgt cactttgggg gaaaatcaaa gaaatattca aaattccttc atctgatacg
    48721 atccctaatc cacctgaatt tctgatcact ggagcgtaac ctgcatcttt cagtgtttgg
    48781 atcccgtcac ttagaaaagg agttcgaaca tccttcatac ctaagatgac tgtgcagtca
    48841 tattgccaga aatgtaaaaa gatttgctgg tgttctttag aatattccgt taatatatct
    48901 gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt gctgatctag tcgaatcgca
    48961 gggttcattc aaccggctcc tttgtataaa ttcatatttt ctacaatatt atactctaaa
    49021 atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg gttgacaaaa
    49081 caaaagaaac taggaacaat gtagctatga gaagtaaagg agaatgaata atggaattat
    49141 cacaaggaac accgatttca atcaaattgc gtacaaaagt aaaacagcaa ggggaagtgc
    49201 aagattttta ttttgatttg aaagggcaaa tggtgacaat tggcgatacg ctgtacatac
    49261 gttataaaga gattcaggag gagacgggcg atgagattcc tgtaacgatc aaattagttc
    49321 cagatggtca tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg aaatttggct
    49381 ataaagaacg gttagaaaca tcctatcgaa caccatacgg tatgatccag attgctactt
    49441 ttacgaaaga actgcatgtt agtttgaaag accgtcccac agcagggaag gttcgaatcg
    49501 attatgatct gttcatggga ccggaaaaaa tcggcgaata ttatttgacc ttggatttta
    49561 cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg attttgtatg ataaggagta
    49621 gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca aacaaaagcg
    49681 aattatcaat gatcgaagta gcacacgcga tcttagaaca acgtggcgat gtcatggatt
    49741 tttctgattt agctaaccag atccaaaact atcttgggaa atcagacagt gaaattcgtg
    49801 attcactcgc acaattttat actgacttga atattgacgg cagctttatc tcattagggg
    49861 ataatcgttg gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa gaagtaaacc
    49921 atggaatgga cgaagaggat gaagatactc cacgtcgccg taaacgcaag aaagtcaacg
    49981 cctttatcaa tgatgatgaa gacgcgatcg attacaatga cgatgaccca gaagatacag
    50041 atctgacaga agaagatgac gatgatctgt ttgatgacga tgatgatgaa gatgaagaaa
    50101 tcgctgctta taattctgac ttgcaagaaa tcggagcaga tgacccgaca gatgatgaag
    50161 aagacttgcc aggaaatatc gaagaagatt taagtatcat agacgaagac gatgatgatg
    50221 aagattatga agaggaagaa ttttctgact ctgaaaaaga ataaacatta ttttcttacg
    50281 agaggttgtg acagagacga tcagcctatc agaataagga ggtgtgacac gatttttgtc
    50341 acaccttctt ttttgtatat aaaaatccca agtaaataca taaaaaataa aaaaattgaa
    50401 acttttagcg caccttgtac gtcatattaa gtgaggcaga aaggagtgga gaatgatttt
    50461 tactggatat aagaaaaaac ggcagatcga aaaataccgt cacctgctga atggactcat
    50521 cgaaactgat tatcaaaaaa tgttccgtat cacgatgaat tacgtacatg ataaagaaga
    50581 ggccttagat gtgatgcaag atagttttca taaagccctg aactattttg aaaaagaaaa
    50641 agagatcgag cattttagtg catggtttta tcggatattg atccgcacgg cattagatag
    50701 ttggcgcaga caaaaaagga cgcctgtgga cttatttgaa attcaagata tcccccaaca
    50761 gttaccttta gaaacttccg ttgcagagct ccatatgatt ttagataaaa tagacagtcc
    50821 ggaaaaagag attctgatcc tctacttttt tgaaggattt cgtttgaaag aaattgcgac
    50881 tatcttggat atgaatgaaa acacagttaa aacgaaaatg taccgctcat tgaatgcttt
    50941 acgtcaaatc ctagaatgaa ggagagaaga gtgccatgta ttcccaaaaa gaaaatttaa
    51001 aaaaactaaa gaaaacttat aaagaacaag caattcccaa agggcttaaa gaggagatcc
    51061 gccagcgttt tattatcgaa gagcaatcat ttataaaagc aaagcggaga aagagacgtc
    51121 tccaagggtt cggagtatct ttggctatcg taatgattac aacaagtagc cttcttttta
    51181 atagtcaagt tcgaagcttt gcagaagatt tacccatctt aggatcagtc attgaattga
    51241 tcttaggcga acgctttact gatcgatctg aaaaaatcga tattcaggta ccgaagatca
    51301 atacacaaaa tgagaaagaa aataaaacaa ttcatggatt aaatcaaaaa tactttcgcg
    51361 aaggacaagc agattttgaa aaagctgaaa aagaatatgg aaattttgaa acggatcatt
    51421 accaagtttt gggagattat cagaaaatcg tagacgataa ccggtttctc gtgattgaaa
    51481 ggcagatcac ccaaacagca gcagatagcc atgttgaaaa aagatacgac acaatcgaca
    51541 agaaaaacag tgtccagctc tcacttccac ttttattcaa agatgacacc tatctatctg
    51601 ttttgacaaa agaagtcaaa cggcagatgg ccgaacaagt caaggaagat ccatctaagt
    51661 actattggac tgagcaagat attcaagaag ggacgataga aaaaccaaca cttgttacgc
    51721 ctacacgaag tttttatttg aacaaagagc atcaattagt tttgactttt tctcaatacg
    51781 aaattgctcc tggttatatg ggtacaccag aatttgtcat tcctaagtcc gtgacaaaaa
    51841 cgattttggc atcggaagat tatttggatc attagacgag aagtagttgc gactagcagt
    51901 ttcaattttc aagtaagtac aagtaaataa taagaagaga aaaagagaaa gaagagatat
    51961 agtgaaaaga aattatttag ttatcagtat tattgcttta tccgtgcttt tgtttttcga
    52021 aattacttcg gtggcctcct tgatcaccga agtaaaagga caaacacact cttcggagca
    52081 ttcgtcacaa tctttcaaac aatcagaaca atcaaccatt aaaagccaat caactgaaac
    52141 agctaacgtc aaagaagcca ttcaagaatc agcggaaaag gtacaaagtc agtcatcaca
    52201 atcaacacaa gacagtacaa aaacgagccg agatccggct gtcaaaagcg tcgcaatcag
    52261 ctttgatgat ggaccaggag cgaccacaac accgcagctt ctgcgaatct taaaagaaaa
    52321 aaacgtgcat gccacttttt tcgtcttagg agaaaatacg gcgcagcatc ctgaaatcgt
    52381 gaagcagaca gctgaagcag ggcacgagat tggcaatcac acatacgatc atcagaatct
    52441 ggccactctt tctgcccagt cgatgacgga ggaagtgacg aaagcagaca ctgagatcaa
    52501 aaaagtcact ggcaaaaccc ccacttttgt tcgtccgccc tatggttctg tcacgaatgt
    52561 aggagcaacg atcatccagc gaccgatcat cgaatggtct gttgattcag aagattggaa
    52621 gacaagaaat cctgatttga ttttgcagaa gatccaagca acagtatacg atggggcaat
    52681 tattttattc catgatatct atcctgaaac gattcgtgcc gttcctcaag tgatcgatta
    52741 tctgaaagag caagggtatc gcatcacgac agttggggat ttattagggc atccaacggc
    52801 tgtcgagaac tattatggca gaaacgatca tcggccagtt cagtaagtga gacaagtatc
    52861 tttaataaat aacacatttt aataagtagt tgaacacata actaccttac ttgcaaaggc
    52921 ttaacaatat ctatacaggt acaacaaaaa actgtccacc aattttcttt ggcggacagt
    52981 tttcacttaa aaataaagaa gcaactattc gtaaattatg ctaaaatagc agtagaacgc
    53041 gtcctgaggg aatcgaaccc ccgtctcaag aaccggaatc ttacgtgata tccactacac
    53101 taaggacgca agcactggga taattgtaac taaacagata aaaaattaca aggggattgg
    53161 acaaaaaaat gaaatttgag caaaattttt cacaaaaaca gcagcagaca cagaaactag
    53221 ccatgacgca acagttgcaa cagtccatcc aagttttaca atataacagt gaggagctgt
    53281 tggcattcgt tgagaatcaa gcaatggaaa atccattggt ggaagttgtt gagccggaat
    53341 ggcagccaga ttacataaaa gcttcttcat cttcttatga aggagaagag acaaattacc
    53401 taaatcagat tcctgataca aaagggtctt tatttgattc attgatcgaa caagtccatt
    53461 tgaattaccg agatactttt ttgaggaaaa tcgttcttta tcttgtagaa tacatcgatt
    53521 tgaacggatt tttgacgatt agtttagaag aagccggaaa agagattgga gctacgccta
    53581 tccaaatgct cgatgcgttg actctgattc agcaattaga accagctgga gtaggtgcac
    53641 gtagtttgca agaatgtctc atgcttcaaa cagaacgaga tgattatgcg ccggaattag
    53701 cttatatcgt gttggaagaa tgttttgagg aactggtgga gcgaaaatgg aaagaaatcg
    53761 cacaacgttt ttctgttgat ctacatgctg ttcagcagat attcgactat ttacaaacgt
    53821 tgagtccaag ccctggccgg attttcgacc gtagcagcga attgtacatt attcctgatg
    53881 tccgggtatt agtggacgac gataaaaatg tacaagttat ctcaaaccga aaaaatcagc
    53941 caaatatacg ctttcaagag agttacttca aacaaatgtc acaacaagca gataaagaaa
    54001 ctgaaaatta tttaaaagaa cggaaacagg aattcgaatg gttgaagaaa acgatcctac
    54061 aacgtgggga tacaattttg cgggttgctc aagtcattgt ttcccggcaa aaagctttct
    54121 ttatcgacaa ggaacgtcca atcaagcctc ttacgttaaa ggaagttgca actgagatag
    54181 atgtgcatga atccacggtc agtcgtgcag tcaacgggaa atatttggaa acagacttcg
    54241 gtgtatttga attaaaaaaa tttttcacta cacgaattcc tacgaatagt acagaacaga
    54301 cagaagacct ttcagcagat acagcgaaga aaaagctgca ggaattagtc gatcaagaag
    54361 acaaaaacaa gccgttgtct gaccagaaat tggttgaatt gctgaaaaaa gatgagatag
    54421 cgatctcaag acgaacagta gcaaaatatc gtgatttatt aggaatcccg agttcatcaa
    54481 aaagaaaacg ttatgataac taatcagtca aaagactaga aaaagtattt cactttttct
    54541 agtcttttta gttgaaaaac gagagcaaaa ctgttagact tacagtcgtg aggttgagat
    54601 ttccctttat tgatgtgaaa ttcgaatagt taatttcttt ttttatcgta tatgggtcgt
    54661 tttaagtcta cattggacgt aaagtgtcca actaaggaga tcgctatgct ggatgaaata
    54721 aaaatgattg aatctgttgc accagatatt atcgaagtgt tgcaagaacg ctataaaatt
    54781 ttacgaaata tctattggat gcagccgatt ggacgtcgaa gtttatcgga aagtatgggg
    54841 atcacagaac gagtattgcg aacagaaaca gatcttttga aaaatctgaa tttgatcgac
    54901 acctcaaaaa gcggtatgac gctgacaact aagggcgaag aagtttatca aagccttgaa
    54961 aacttcatgg atcagctttt aggtacgcat caaaccgaac aacagttggc agaatatttt
    55021 ggtaaccaac gctgtatcgt tgtttcagga aacagcgaag agcagaccaa agtggctgat
    55081 gcattcggcg aagcgctgag cgaagcccta gaccgactac ttccagaagg cgagaatatc
    55141 atcgctgtga tgggaggaac tactatggct gtggtagccg aacaattgtc taatctggaa
    55201 aataagaaac ggcataacct gtttgtccct gcaagaggcg ggattggtga agcgataact
    55261 gtacaggcaa attctgtcag tgcaagaatg gctgctaaag caaacggtaa ccaccgtgca
    55321 ctttatgttc cggaacaatt aagtctggca acttacaatt ctttactcaa tgagccatct
    55381 atccaagaag tcttaaactt gattagtgaa gcgaattgtg ttattcatag tattgggcgt
    55441 gcattacaca tggcagcacg tcgtaagatg actgaaaaag aattagtcat gctgaaacaa
    55501 gcaaatgctg ttgcggaatc ttttggctac tttttcaatg aaaaaggtga agtcgtttat
    55561 aaggtcccta gaatcggcat agaattgcaa gatttggaaa aagtcccgat catcatggca
    55621 atcgcgggcg gaaaatcaaa agctaaagca attcgagctt atatgaaaaa tgcaccaaaa
    55681 caaacgtggc tcatcactga tgaagctgct gcaaatgaga ttttaaaagg ggtaaccctt
    55741 taaaataaat atttttttga ttttcataag gaggaaatct taaatgacag ttaaagtagg
    55801 tattaatggt tttggacgta ttggacgtct agcattccgt cgtatccaag atgtagatgg
    55861 aatcgaagta gtagcaatca acgacttgac agatgctaaa atgttggcac acttgttaaa
    55921 atacgacaca actcaaggac gtttcaacgg aacagttgaa gttcatgaag gttcattcaa
    55981 cgtaaacgga aaagaagtta aagtattagc taaccgcaac ccagaagaat taccatgggg
    56041 cgaactaggc gtagatatcg ttctagaatg tactggtttc ttcacttcta aatcagcagc
    56101 tgaaaaacat ttaacagctg gtgctaaacg cgttgttatc tctgctcctg gcggaaacga
    56161 tgttccaaca atcgtttata acacaaacca cgaaacatta actggtaaag aaacagttat
    56221 ctcaggtgct tcatgtacta ctaactgttt agctcctatg gctaaaacat tgaacgacaa
    56281 atttggtgtt gttgaaggat taatgacaac tatccacgct tacacaggtg accaaatgac
    56341 tctagacgga cctcatccta aaggtgactt ccgccgtgca cgcgctgctg ctgcaaacat
    56401 cgttcctaac tcaacaggtg ctgctaaagc tatcggttta gtaattcctg aattgaacgg
    56461 taaattagac ggagctgctc aacgtgttcc tgtaccaaca ggttcattaa cagaattagt
    56521 aacagttctt gaaaaagaag taactgttga cgaaatcaat gcagcaatga aagaagcttc
    56581 aaacgaatct tacggataca acacagacga aatcgtttct tcagatatcg ttggtatgac
    56641 ttacggttca ttatttgatg ctacacaaac taaagtaatg acagttggcg acaaacaatt
    56701 agttaaaact gttgcttggt acgacaacga aatgtcatac actgcacaat tagtacgtac
    56761 tttagaatac ttcgctaact tataagatag acaaacgtac aagtgtaaat tatagacatg
    56821 tacatttaaa agcggggaag caatcgcgct tcttccgctt tttttgataa taagtttatt
    56881 caggaggaaa taaaatggct aagaaaacag tgaaagatat cgatttaaaa gacaaaaaag
    56941 ttcttgtccg tgttgacttc aacgttcctt tgaaagacgg cgtgatcact gacgacactc
    57001 gtatcaaagc agcattgcca acaatcaact acgttctaga acaaggcgga aaagcaatcc
    57061 ttttctctca ccttggacgt gtaaaaacag aagaagataa agaaggaaaa tctttagctc
    57121 cagttgcaaa acgtctaggc gaattattag gtaaagaagt aacattcgta cctgaaactc
    57181 gcggagaaca attagaagaa gcaatccgca acatgaatga cggcgacgtt gtcgtattcg
    57241 aaaatacacg atttgaagat attgacggta aaaaagaaag caaaaatgat acagaactag
    57301 gtaaatactg ggcttcatta ggcgatgtat ttgttaacga tgctttcggt acagctcacc
    57361 gtgcacatgc ttctaacgta ggtatcgctt caactggtat cccaacagtt gctggattct
    57421 tgatggaaaa agaaatcaag ttcatcggtg aagcagtaga agaaccaaaa cgaccaatga
    57481 ttgcaatcct tggtggcgca aaagtttctg ataaaatcgg cgtaatcgaa aacttgattc
    57541 ctaaagcaga taaaatcttg atcggtggcg gaatgactta cacattctac gaagcaaaag
    57601 gaatcaagat cggtaactct ctagtagaag ctgataaagt ggaactagca aaagaattaa
    57661 tcgaaaaagc aggcgacaaa cttgtcttgc caatcgacaa tgtctgtgca ccagaatttt
    57721 caaatgatgt tgaaacacaa gtaatcgaag gagacattcc tgatggtttg atggctttag
    57781 acatcggccc agcatcagta aaattatttg ctgatacatt acaaggtgca aaaacagttg
    57841 tttggaacgg acctatgggt gtatttgaaa tgagtaactt tgctaaaggt acaatcggtg
    57901 tttgtgaagc aatcgctaac ttagaaggcg caacaacaat catcggtggc ggagactcag
    57961 ccgcagcagc agaacaatta ggatttgctg acaaattcac acacatctca acaggtggcg
    58021 gcgcaagctt ggaattgctt gaaggtaaag aattaccagg actagcagca atcaacgata
    58081 aataaacgac caagtttgct caattctgac gagtaatcga acaaaaggtg aagggagttt
    58141 tcatgactcc ctagccattt ttcaataaat taaggagtgt gttcctcaat gcgtaaacct
    58201 attatcgcag gtaactggaa aatgaacaaa actgcatcag aagcaaaaga atttgctgaa
    58261 gcagtaaaaa acaaaatccc ttcaaatgac aaagttgatt cagtcatcgg atctcctgca
    58321 ttattcttac aagaattaat gacatcagct gaaggtacag aactaaaaat ctcagcacaa
    58381 aactgctact gggaaaactc aggtgcgttc actggtgaaa cttcaccagc agctcttgct
    58441 gatctaggtg ttcattatgt gatcatcgga cattcagaac gtcgtgaata tttccatgaa
    58501 acagacgaag acatcaataa aaaagcgaaa gctatctttg caaatggtat gacaccaatc
    58561 ctatgctgtg gcgaatcttt agaaacctat gaagcaggca aaactgctga atggatcgaa
    58621 ggacaaatta cagctggttt agctgatctt tcagctgaac aggtgtcaaa catggttatt
    58681 gcttatgaac caatctgggc aatcggtact ggtaaatcag ctgacgcaca aatcgctgac
    58741 gaaatttgtg gagttgtacg tcaaacagtt gaaaaacttt atggaaaaga agtatcagaa
    58801 gcagttcgta tccaatacgg cggttctgta aaaccagaaa atattgctga atacatggca
    58861 aaagaaaatg ttgacggtgc tttagtcggt ggcgcaagct tgcaagcaga ttcattcttg
    58921 tcattgttag acgctgtaaa ataattgttg aaaatttgcg aaaaatgcaa gaaatgtttg
    58981 cactaatgag caaaattaac taaaatcgta tatgagggaa atcccttagg aaaaaacaaa
    59041 ctcaaaggag agacaaaaca tgtcaattat tactgatgtt tacgctcgcg agatcttaga
    59101 ctcacgtggt aacccaacaa tcgaagtaga agtatacaca gaaagcggag cttttggccg
    59161 cggaatggtt ccttcaggtg cttcaactgg tgaatacgaa gcagttgaat tacgtgacgg
    59221 agacaaagct cgttatggcg gtaaaggtgt aactaaagct gtcgacaacg taaacaacat
    59281 cattgctgaa gcaatcattg gctacgatgt tcgtgaccaa atggctatcg acaaagctat
    59341 gatcgcttta gacggaactc ctaacaaagg taaattaggt gcgaacgcta ttttaggtgt
    59401 ttctatcgct gttgcacgtg cagctgctga ctacctagaa gtacctttat accactacct
    59461 aggcggattc aacacaaaag tattgccaac accaatgatg aacatcatca acggcggatc
    59521 acatgctgat aactcaatcg acttccaaga attcatgatc atgcctgttg gcgctccaac
    59581 attcaaagaa gctctacgta tgggtgctga agtattccac gcattagctt caatcttgaa
    59641 agctcgcgga ttagcaactt ctgttggtga cgaaggtgga tttgctccaa accttggttc
    59701 aaacgaagaa ggttttgaag taatcatcga agcaatcgaa aaagctggct atgtacctgg
    59761 taaagacgtt gttcttgcta tggatgctgc ttcttcagaa ttctacgaca aagaaaaagg
    59821 tgtttacgta ctagctgatt caggcgaagg cgaaaaaaca actgacgaaa tgatcaaatt
    59881 ctacgaagaa ttagtttcta aatatccaat catctcaatc gaagacggat tagacgaaaa
    59941 cgactgggac ggattcaaga aattaactga cgtattaggc gacaaagttc aattagttgg
    60001 tgacgacttg ttcgtaacaa acactcaaaa attatctgaa ggtatcgaaa aaggaattgc
    60061 taactcaatc cttatcaaag tgaaccaaat cggtacatta acagaaactt tcgaagctat
    60121 cgaaatggct aaagaagctg gctacacagc agttgtatct caccgttctg gtgaaacaga
    60181 agattcaaca atctctgata tcgctgttgc aacaaacgct ggccaaatca aaactggttc
    60241 tctatcacgt actgaccgta ttgctaaata caaccaatta ctacgtatcg aagaccaact
    60301 tggtgaagtt gcagaataca aaggtttgaa atctttctac aacttaaaaa acaaataatt
    60361 cttttctaat gaagatacat gatcgactat tgaaagatag tcgagtatga tcacttagtt
    60421 aagtaatcga actctctact tgctgaaata tcagcaggta gagagttttt ttgcaactag
    60481 cttctgtata tgatgtttac aaagaatggt acaagaaaag atcatccttg aaaaaggaaa
    60541 gctaacaata actagcatca tataagagct tttaaggata gaacagtaga ataggaatct
    60601 atgagcaagt cctctcgtat aaataaaata ggagggaagt agcagcatat ttttcataaa
    60661 gtggctctct ttcttcattt ttactttggt tgctcctatt tgaaaattcg agcacactca
    60721 aatacaataa aaagaaatgg caagggagag gattagatga aaaaagaaga aaaaatcgcg
    60781 attttacaag agatcatccg aattaaatca gtgaatggaa acgaaggtga agtagcagcc
    60841 tatttgaata agcttttggc aaagcatgac ataacaggag agattgtttc ttacagagat
    60901 ggtagagata atttgattgc tcgttaccaa aagggccaat caggaaaagt gttaggccta
    60961 tctgggcata tggatgtcgt ggcggctggt gatgaatcgt cttggaccta tgcacccttt
    61021 gcagcagaaa tccatgggaa ccgtttgtac ggacgtggtg ctacagatat gaagagtggc
    61081 ttggcagcaa tggtgattgc catgatcgaa ctgaaagagt caggaaaacc atttaacgga
    61141 acagtcaaat tattagccac tgtcggagaa gaagtgggtg aactaggtgg ggaacagctc
    61201 acgaaagcag gctatgtaga tgacttagat gccttgatca tcggagagcc aaccaattac
    61261 agtttgatgt atacgcacat gggatcaatc aactatacag tcacctcgca cggaaaagaa
    61321 gcgcatagct ctatgccaga ccaaggttat aatgcaatca atcacctaaa tgaattcatt
    61381 acgaaagcaa atgctgaaat gaatcacctc gctgaaacaa tcgagaatcc tgtcctagga
    61441 aaaacaatcc ataatgttac attgatcagt ggcgggaatc aagtcaacag catcccaagc
    61501 catgcgcagc tccaagggaa cattcgatcg attccagaat acccaaatga taaaatcatt
    61561 gcattgctgc aatcaatcgt caatgaattg aatcaagaga cagactatca tttggaattg
    61621 atgatcgatt acaacaaaat acctgtcaaa gctgatccag attctccttt gattcattgc
    61681 attcagcaac aattcagcca gccattacct ttagttggag cggcagcaac aacggatgca
    61741 gccgaattta cgaaagctaa ccattccttc gactttgtcg tctttgggcc tggtgtcgtt
    61801 actttgcctc atcaagtaga tgagtatgtt gaaatcgata attatttgga tatgatcgaa
    61861 aaatatcaag gaattatttt atcctactta gcgtaagatt tttttaataa atgaaaaaac
    61921 agagccagat tagcattatt t
//
LOCUS       DACJGB010000008        61858 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.008, whole genome shotgun sequence.
ACCESSION   DACJGB010000008 DACJGB010000000
VERSION     DACJGB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.008"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(233..1081)
                     /locus_tag="IYI34_000536"
     CDS             complement(233..1081)
                     /locus_tag="IYI34_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR0558433.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IYI34_000537"
     CDS             complement(1305..3245)
                     /gene="thrS"
                     /locus_tag="IYI34_000537"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAR0558434.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3582..3761)
                     /locus_tag="IYI34_000538"
     CDS             complement(3582..3761)
                     /locus_tag="IYI34_000538"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAR0558435.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IYI34_000539"
     CDS             complement(3808..5631)
                     /gene="cadA"
                     /locus_tag="IYI34_000539"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAR0558436.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5909..8188
                     /locus_tag="IYI34_000540"
     CDS             5909..8188
                     /locus_tag="IYI34_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0558437.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8281..8928)
                     /locus_tag="IYI34_000541"
     CDS             complement(8281..8928)
                     /locus_tag="IYI34_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558438.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9107..9826)
                     /locus_tag="IYI34_000542"
     CDS             complement(9107..9826)
                     /locus_tag="IYI34_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR0558439.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9957..11108)
                     /locus_tag="IYI34_000543"
     CDS             complement(9957..11108)
                     /locus_tag="IYI34_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAR0558440.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11325..12065)
                     /locus_tag="IYI34_000544"
     CDS             complement(11325..12065)
                     /locus_tag="IYI34_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0558441.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IYI34_000545"
     CDS             complement(12069..12392)
                     /gene="rsfS"
                     /locus_tag="IYI34_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAR0558442.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12449..13042)
                     /locus_tag="IYI34_000546"
     CDS             complement(12449..13042)
                     /locus_tag="IYI34_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR0558443.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(13026..13673)
                     /locus_tag="IYI34_000547"
     CDS             complement(13026..13673)
                     /locus_tag="IYI34_000547"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAR0558444.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IYI34_000548"
     CDS             complement(13686..13997)
                     /gene="yhbY"
                     /locus_tag="IYI34_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAR0558445.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IYI34_000549"
     CDS             complement(14013..15122)
                     /gene="yqeH"
                     /locus_tag="IYI34_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAR0558446.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15119..15646)
                     /locus_tag="IYI34_000550"
     CDS             complement(15119..15646)
                     /locus_tag="IYI34_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAR0558447.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15826..16611)
                     /locus_tag="IYI34_000551"
     CDS             complement(15826..16611)
                     /locus_tag="IYI34_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAR0558448.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16604..17473)
                     /locus_tag="IYI34_000552"
     CDS             complement(16604..17473)
                     /locus_tag="IYI34_000552"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAR0558449.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17474..18853)
                     /locus_tag="IYI34_000553"
     CDS             complement(17474..18853)
                     /locus_tag="IYI34_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAR0558450.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IYI34_000554"
     CDS             complement(18861..19280)
                     /gene="fabZ"
                     /locus_tag="IYI34_000554"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAR0558451.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IYI34_000555"
     CDS             complement(19305..19781)
                     /gene="accB"
                     /locus_tag="IYI34_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAR0558452.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IYI34_000556"
     CDS             complement(19784..21019)
                     /gene="fabF"
                     /locus_tag="IYI34_000556"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAR0558453.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IYI34_000557"
     CDS             complement(21038..21775)
                     /gene="fabG"
                     /locus_tag="IYI34_000557"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAR0558454.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IYI34_000558"
     CDS             complement(21787..22704)
                     /gene="fabD"
                     /locus_tag="IYI34_000558"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAR0558455.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22811..23035)
                     /locus_tag="IYI34_000559"
     CDS             complement(22811..23035)
                     /locus_tag="IYI34_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAR0558456.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(23088..24050)
                     /locus_tag="IYI34_000560"
     CDS             complement(23088..24050)
                     /locus_tag="IYI34_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAR0558457.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(24060..24500)
                     /locus_tag="IYI34_000561"
     CDS             complement(24060..24500)
                     /locus_tag="IYI34_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0558458.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24806..25771
                     /locus_tag="IYI34_000562"
     CDS             24806..25771
                     /locus_tag="IYI34_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAR0558459.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25828..26058)
                     /locus_tag="IYI34_000563"
     CDS             complement(25828..26058)
                     /locus_tag="IYI34_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAR0558460.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(26144..27031)
                     /locus_tag="IYI34_000564"
     CDS             complement(26144..27031)
                     /locus_tag="IYI34_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAR0558461.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(27120..28343)
                     /locus_tag="IYI34_000565"
     CDS             complement(27120..28343)
                     /locus_tag="IYI34_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAR0558462.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28360..29439)
                     /locus_tag="IYI34_000566"
     CDS             complement(28360..29439)
                     /locus_tag="IYI34_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAR0558463.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IYI34_000567"
     CDS             complement(29570..31297)
                     /gene="ptsP"
                     /locus_tag="IYI34_000567"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAR0558464.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31297..31563)
                     /locus_tag="IYI34_000568"
     CDS             complement(31297..31563)
                     /locus_tag="IYI34_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAR0558465.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            32042..34276
                     /locus_tag="IYI34_000569"
     CDS             32042..34276
                     /locus_tag="IYI34_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAR0558466.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34424..34744
                     /locus_tag="IYI34_000570"
     CDS             34424..34744
                     /locus_tag="IYI34_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAR0558467.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34949..35173)
                     /locus_tag="IYI34_000571"
     CDS             complement(34949..35173)
                     /locus_tag="IYI34_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAR0558468.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35371..36951)
                     /locus_tag="IYI34_000572"
     CDS             complement(35371..36951)
                     /locus_tag="IYI34_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAR0558469.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37352..38728)
                     /locus_tag="IYI34_000573"
     CDS             complement(37352..38728)
                     /locus_tag="IYI34_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAR0558470.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38856..39974)
                     /locus_tag="IYI34_000574"
     CDS             complement(38856..39974)
                     /locus_tag="IYI34_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR0558471.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            40116..40550
                     /locus_tag="IYI34_000575"
     CDS             40116..40550
                     /locus_tag="IYI34_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0558472.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40628..41161)
                     /locus_tag="IYI34_000576"
     CDS             complement(40628..41161)
                     /locus_tag="IYI34_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAR0558473.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41255..42433)
                     /gene="mutY"
                     /locus_tag="IYI34_000577"
     CDS             complement(41255..42433)
                     /gene="mutY"
                     /locus_tag="IYI34_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAR0558474.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42426..43223)
                     /gene="recX"
                     /locus_tag="IYI34_000578"
     CDS             complement(42426..43223)
                     /gene="recX"
                     /locus_tag="IYI34_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAR0558475.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43330..44712
                     /gene="rlmD"
                     /locus_tag="IYI34_000579"
     CDS             43330..44712
                     /gene="rlmD"
                     /locus_tag="IYI34_000579"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAR0558476.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44886..45464)
                     /locus_tag="IYI34_000580"
     CDS             complement(44886..45464)
                     /locus_tag="IYI34_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAR0558477.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45786..46256
                     /locus_tag="IYI34_000581"
     CDS             45786..46256
                     /locus_tag="IYI34_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAR0558478.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46364..47590)
                     /locus_tag="IYI34_000582"
     CDS             complement(46364..47590)
                     /locus_tag="IYI34_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAR0558479.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48344..48544)
                     /locus_tag="IYI34_000583"
     CDS             complement(48344..48544)
                     /locus_tag="IYI34_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR0558480.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48678..49286)
                     /locus_tag="IYI34_000584"
     CDS             complement(48678..49286)
                     /locus_tag="IYI34_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAR0558481.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49371..49841)
                     /locus_tag="IYI34_000585"
     CDS             complement(49371..49841)
                     /locus_tag="IYI34_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAR0558482.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49889..50803)
                     /locus_tag="IYI34_000586"
     CDS             complement(49889..50803)
                     /locus_tag="IYI34_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAR0558483.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50949..51752
                     /locus_tag="IYI34_000587"
     CDS             50949..51752
                     /locus_tag="IYI34_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAR0558484.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51930..52877
                     /locus_tag="IYI34_000588"
     CDS             51930..52877
                     /locus_tag="IYI34_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAR0558485.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52945..53238)
                     /locus_tag="IYI34_000589"
     CDS             complement(52945..53238)
                     /locus_tag="IYI34_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAR0558486.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(53266..53931)
                     /locus_tag="IYI34_000590"
     CDS             complement(53266..53931)
                     /locus_tag="IYI34_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAR0558487.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(54071..54703)
                     /locus_tag="IYI34_000591"
     CDS             complement(54071..54703)
                     /locus_tag="IYI34_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0558488.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54710..55777)
                     /locus_tag="IYI34_000592"
     CDS             complement(54710..55777)
                     /locus_tag="IYI34_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR0558489.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55774..56505)
                     /locus_tag="IYI34_000593"
     CDS             complement(55774..56505)
                     /locus_tag="IYI34_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAR0558490.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56674..57153)
                     /gene="greA"
                     /locus_tag="IYI34_000594"
     CDS             complement(56674..57153)
                     /gene="greA"
                     /locus_tag="IYI34_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAR0558491.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57289..58464)
                     /gene="mltG"
                     /locus_tag="IYI34_000595"
     CDS             complement(57289..58464)
                     /gene="mltG"
                     /locus_tag="IYI34_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAR0558492.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58668..59837
                     /locus_tag="IYI34_000596"
     CDS             58668..59837
                     /locus_tag="IYI34_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAR0558493.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(60104..61726)
                     /locus_tag="IYI34_000597"
     CDS             complement(60104..61726)
                     /locus_tag="IYI34_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558494.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        18132 a        12841 c        10358 g        20527 t
ORIGIN      
        1 aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa tcaacttttt
       61 gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa aaaataaact
      121 tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca tttatttgtg
      181 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
      241 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      301 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      361 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      421 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      481 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      541 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      601 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      661 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      721 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      781 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      841 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      901 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      961 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
     1021 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
     1081 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
     1141 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1201 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1261 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1321 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1381 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1441 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1501 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1561 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1621 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1681 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1741 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1801 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1861 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1921 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1981 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     2041 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     2101 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     2161 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2221 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2281 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2341 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2401 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2461 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2521 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2581 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2641 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2701 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2761 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2821 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2881 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2941 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     3001 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     3061 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     3121 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3181 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3241 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3301 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3361 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3421 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3481 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3541 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3601 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3661 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3721 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3781 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3841 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3901 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3961 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     4021 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     4081 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     4141 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4201 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4261 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4321 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4381 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4441 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4501 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4561 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4621 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4681 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4741 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4801 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4861 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4921 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4981 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     5041 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     5101 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     5161 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5221 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5281 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5341 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5401 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5461 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5521 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5581 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5641 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5701 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5761 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5821 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5881 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5941 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     6001 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     6061 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     6121 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6181 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6241 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6301 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6361 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6421 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6481 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6541 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6601 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6661 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6721 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6781 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6841 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6901 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6961 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     7021 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     7081 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     7141 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7201 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7261 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7321 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7381 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7441 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7501 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7561 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7621 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7681 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7741 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7801 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7861 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7921 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7981 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     8041 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     8101 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     8161 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8221 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8281 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8341 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8401 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8461 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8521 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8581 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8641 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8701 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8761 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8821 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8881 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8941 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     9001 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     9061 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     9121 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9181 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9241 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9301 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9361 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9421 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9481 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9541 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9601 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9661 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9721 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9781 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9841 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9901 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9961 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
    10021 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
    10081 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
    10141 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10201 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10261 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10321 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10381 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10441 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10501 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10561 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10621 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10681 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10741 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10801 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10861 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10921 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10981 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    11041 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    11101 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    11161 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11221 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11281 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11341 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11401 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11461 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11521 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11581 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11641 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11701 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11761 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11821 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11881 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11941 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    12001 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    12061 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    12121 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12181 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12241 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12301 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12361 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12421 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12481 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12541 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12601 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12661 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12721 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12781 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12841 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12901 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12961 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    13021 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    13081 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    13141 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13201 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13261 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13321 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13381 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13441 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13501 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13561 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13621 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13681 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13741 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13801 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13861 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13921 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13981 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    14041 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    14101 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    14161 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14221 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14281 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14341 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14401 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14461 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14521 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14581 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14641 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14701 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14761 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14821 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14881 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14941 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    15001 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    15061 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    15121 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15181 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15241 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15301 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15361 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15421 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15481 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15541 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15601 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15661 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15721 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15781 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15841 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15901 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15961 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    16021 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    16081 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    16141 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16201 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16261 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16321 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16381 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16441 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16501 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16561 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16621 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16681 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16741 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16801 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16861 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16921 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16981 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    17041 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    17101 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    17161 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17221 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17281 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17341 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17401 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17461 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17521 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17581 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17641 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17701 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17761 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17821 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17881 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17941 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    18001 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    18061 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    18121 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18181 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18241 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18301 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18361 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18421 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18481 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18541 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18601 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18661 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18721 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18781 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18841 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18901 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18961 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    19021 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    19081 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    19141 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19201 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19261 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19321 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19381 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19441 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19501 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19561 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19621 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19681 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19741 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19801 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19861 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19921 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19981 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    20041 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    20101 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    20161 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20221 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20281 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20341 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20401 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20461 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20521 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20581 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20641 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20701 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20761 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20821 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20881 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20941 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    21001 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    21061 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    21121 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21181 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21241 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21301 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21361 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21421 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21481 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21541 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21601 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21661 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21721 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21781 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21841 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21901 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21961 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    22021 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    22081 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    22141 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22201 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22261 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22321 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22381 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22441 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22501 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22561 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22621 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22681 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22741 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22801 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22861 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22921 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22981 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    23041 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    23101 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    23161 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23221 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23281 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23341 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23401 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23461 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23521 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23581 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23641 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23701 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23761 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23821 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23881 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23941 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    24001 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    24061 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    24121 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24181 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24241 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24301 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24361 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24421 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24481 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24541 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24601 tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa
    24661 tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg
    24721 aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt
    24781 tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct
    24841 gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa
    24901 acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt
    24961 attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga
    25021 tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat
    25081 gaattatttc agtaattata attatgtaca tattaccaat aagggatttg tattaacaga
    25141 tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt
    25201 gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt
    25261 gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca
    25321 agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca
    25381 tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt
    25441 tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac
    25501 cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg
    25561 tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa
    25621 gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca
    25681 aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa
    25741 caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc
    25801 aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac
    25861 gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt
    25921 atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg
    25981 aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac
    26041 taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa
    26101 gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg
    26161 gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac
    26221 ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat
    26281 ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa
    26341 aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag
    26401 ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg
    26461 catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca
    26521 tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt
    26581 tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg
    26641 taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt
    26701 tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa
    26761 ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt
    26821 ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt
    26881 ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc
    26941 gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca
    27001 tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc
    27061 ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt
    27121 tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt
    27181 cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg
    27241 ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga
    27301 acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt
    27361 ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa
    27421 ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca
    27481 ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct
    27541 gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca
    27601 taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga
    27661 aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc
    27721 atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg
    27781 atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct
    27841 attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc
    27901 gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat cacttttcct
    27961 ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt
    28021 ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga
    28081 ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca
    28141 actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc
    28201 aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct
    28261 aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta
    28321 tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac
    28381 tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc
    28441 ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact
    28501 cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc
    28561 catatagcag ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag
    28621 tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga
    28681 agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta
    28741 accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg
    28801 aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc
    28861 aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac
    28921 tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg
    28981 gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata
    29041 gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta
    29101 caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatctga taaaggaatt
    29161 tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc
    29221 tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc
    29281 ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc
    29341 aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac
    29401 cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa
    29461 acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa
    29521 aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat
    29581 attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat gcacgatcag
    29641 caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag
    29701 atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt
    29761 gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa
    29821 ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc
    29881 gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga
    29941 agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca
    30001 tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt
    30061 cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca
    30121 tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca
    30181 tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca
    30241 tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa
    30301 caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat
    30361 cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg
    30421 cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta
    30481 attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg
    30541 ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag
    30601 gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt
    30661 tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag
    30721 cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta
    30781 attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca
    30841 ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac
    30901 gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga
    30961 tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat
    31021 tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata
    31081 cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt
    31141 cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg
    31201 ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac
    31261 cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt
    31321 cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt
    31381 aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac
    31441 tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa
    31501 tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc
    31561 catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt
    31621 aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg
    31681 ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt
    31741 tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt
    31801 ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa
    31861 ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata
    31921 gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct
    31981 catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt
    32041 catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa
    32101 tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca
    32161 aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga
    32221 tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc
    32281 aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga
    32341 aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga
    32401 tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac
    32461 caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg
    32521 tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat
    32581 tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg
    32641 tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga
    32701 agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat
    32761 tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga
    32821 gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga
    32881 tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga
    32941 ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg
    33001 ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc
    33061 taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga
    33121 agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta
    33181 tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac
    33241 acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt
    33301 gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc
    33361 tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg
    33421 tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg
    33481 tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc
    33541 aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg
    33601 ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag
    33661 aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca
    33721 tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag
    33781 ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa
    33841 cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa
    33901 ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc
    33961 aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc
    34021 agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg
    34081 ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga
    34141 tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga
    34201 taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga
    34261 agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg
    34321 tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat
    34381 aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt
    34441 aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt
    34501 caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg aacataatga
    34561 aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa
    34621 ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga
    34681 attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt
    34741 gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag aacacttttg
    34801 tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg gaaataagct
    34861 gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc ttggagttaa
    34921 acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt ttcttttgta
    34981 cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt tcctctttat
    35041 ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct tttacatcta
    35101 ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca cgtctttgcc
    35161 attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa aataattttt
    35221 atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc gtcacggaaa
    35281 atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat tcttattact
    35341 tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt ttacattggg
    35401 atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca acggttggtc
    35461 gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca aatcttctgg
    35521 atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg cgttatactc
    35581 atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc caatgatata
    35641 ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta attgatagat
    35701 tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca taaacagctc
    35761 tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga acaatgtatc
    35821 cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta ctgctttttc
    35881 gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca gttttttatt
    35941 tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga ttcggacaaa
    36001 agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata cgaaacctga
    36061 aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc cataaggagc
    36121 tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag tcaatgcaga
    36181 accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa atgcatctcc
    36241 agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat tttcagttag
    36301 aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt catcctgata
    36361 attttccgga cgataaaact ctacacgatt atggtagata tcataaagac cttctagccc
    36421 tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata gctcttctaa
    36481 ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga taaaagtaaa
    36541 tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg cttcgatccc
    36601 ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac gataagtatc
    36661 ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc cttgataatc
    36721 aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt ccatccagtc
    36781 tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac ggatagcccc
    36841 accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat gggaaatgat
    36901 cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca taacctaact
    36961 ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg atctcttaaa
    37021 gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt tttccgagta
    37081 aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa atacgaaaag
    37141 ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac aacctttgtc
    37201 aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc accaaaccat
    37261 aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct tgtttattca
    37321 agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca tcttcgaatt
    37381 cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc ggatcatgaa
    37441 aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc agcgtaatat
    37501 tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat gctgtaatca
    37561 aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa gcttcattga
    37621 actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt ttttcataaa
    37681 gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct aaaaggtctt
    37741 ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc atctgattct
    37801 gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga acgaataaag
    37861 gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg gcagctttta
    37921 gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta taaacaggga
    37981 ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg tcgttgatat
    38041 caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc gtatcaagag
    38101 aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg ttttcaagca
    38161 tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc atcggcgtga
    38221 tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta gctactacac
    38281 caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta tttaaagcaa
    38341 tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata gccagcacaa
    38401 tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct aaccgttccg
    38461 ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt cctacttgga
    38521 ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga gatttcacat
    38581 ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca gcaatttcgg
    38641 aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc tgcgctaaca
    38701 gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa attaattttt
    38761 agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc acaaagcaat
    38821 tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc aattttcttc
    38881 ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat gatcgtcttg
    38941 cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat atttccagct
    39001 actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc aattacatta
    39061 ggataaatga tattcccatc caactgttgg acgattagca caaccacaca acaaagtaat
    39121 gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc tagataaggt
    39181 cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa agcataatcc
    39241 acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca ttcgattgct
    39301 tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc cacaatttgg
    39361 tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc tccgtctttc
    39421 aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga agcgatcgtt
    39481 ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat gattgtcccg
    39541 taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa caaaggcaat
    39601 tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat attagatgcc
    39661 aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca aataattgct
    39721 gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt tgctttcatc
    39781 aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag tacaggtgca
    39841 aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc tgatgaaatc
    39901 atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt agactgtcta
    39961 agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta tattcgttaa
    40021 aaattctttt attctttttt tatttttgaa aacttgccat actttttatt tgttttattt
    40081 tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa acaaaagata
    40141 caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt gtagtcgaac
    40201 agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc cacttcgtcc
    40261 tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta gaaaacggaa
    40321 aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg ttaggaaaaa
    40381 tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg attttattag
    40441 gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct tacggcgatc
    40501 cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa aaatttattt
    40561 cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt actgttcttt
    40621 tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca atatagcctt
    40681 ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca ttttctttta
    40741 ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca tattcatcga
    40801 catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca tagtcgatat
    40861 atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa taggaaaccc
    40921 ccttttctct gatcattgct attacattga accagtattt tttatgaaaa taaacaatcg
    40981 ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg tgatcattca
    41041 cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac gttcgatgca
    41101 atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt ggaactccca
    41161 tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat tacaccgaca
    41221 tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta ttgtttcgat
    41281 ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc gaatcaaatg
    41341 catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct gctccctcgg
    41401 ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata tgagtaactt
    41461 ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg agattttgct
    41521 cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc cattcttttt
    41581 tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac atattcgcca
    41641 gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt tgaagagctg
    41701 cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga aaactcgttt
    41761 ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca cactttggag
    41821 aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat tcacctggat
    41881 gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta gaagcttttg
    41941 cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg ccgtcaactg
    42001 caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat ggtcctattc
    42061 ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat tctgacatga
    42121 tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg ccctcccatg
    42181 cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga aaccattcca
    42241 tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc atgatctcag
    42301 agatccagat acgataagga tcacgattat agcgccaagg caggtttctt ttttcttgtt
    42361 cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc catttttccc
    42421 attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat atccaaatca
    42481 aatccttttt gatataagct ttgctttact tttagatttc tttttttagg ttcaaacctt
    42541 aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc tatttcttga
    42601 tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga aaagcctttc
    42661 gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc gtgatttttt
    42721 ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa tgtatattgg
    42781 ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg ctgcgctaat
    42841 ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc cccatagacc
    42901 aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg atcttcttga
    42961 aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa ctgataactt
    43021 aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt gatctcttgg
    43081 atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa tacatcttca
    43141 gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc tttgccttta
    43201 ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc tatcatacca
    43261 cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa aagaaaaagg
    43321 gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt gaaaatcaag
    43381 cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat atttgttcca
    43441 tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc tcgctatgca
    43501 gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc accatgtcct
    43561 gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc tcaattggat
    43621 tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg ctttcgatct
    43681 tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa caaagcacaa
    43741 tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata cgaagcaaac
    43801 tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac acaaaaagta
    43861 atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga tgaacggaca
    43921 aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg ggagttacaa
    43981 gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat ccgtgagatc
    44041 aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca aaaaacgtct
    44101 ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga agaacaaatc
    44161 aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc tcagcagacc
    44221 gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga aacgatcgtg
    44281 gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc caaagaagta
    44341 cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc ggaaagatta
    44401 ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc taaatggttg
    44461 aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg tttggataac
    44521 gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat ctcctgtaat
    44581 gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat tgaatattta
    44641 cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa gatgacgaga
    44701 ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt aataagaaca
    44761 gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg aagagttaac
    44821 aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac agcccttttt
    44881 gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt atcgctgata
    44941 ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt gatcgtttgg
    45001 ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa ttcaaaatca
    45061 ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta atcccaagta
    45121 cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg attaaccatg
    45181 tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct gactcctgca
    45241 ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg cggttcccat
    45301 tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag gtatgcacgg
    45361 cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc gaacaaccct
    45421 aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa acctccttca
    45481 tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc ttttataatt
    45541 ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga agtagacgag
    45601 aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa aacatcattc
    45661 atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc ttacttcaca
    45721 aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag aaaggagtta
    45781 gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga attgtcatat
    45841 taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat tctttgcaac
    45901 ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta ctgtatatat
    45961 tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag cgaaacaatc
    46021 ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct ctatttttta
    46081 atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt ttcttgtcag
    46141 tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg attccttatt
    46201 atttatgggt actattcgca actctattga attattcaat ttatttttta aactaagcgc
    46261 taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt tagatttttt
    46321 gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac ccacttgttg
    46381 ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca gcgtaaggat
    46441 cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct gtttgctgat
    46501 aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa gaataatcgg
    46561 tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc gaatcatcaa
    46621 agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt gtttttatat
    46681 ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct aaaatttgat
    46741 agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg attgtttcaa
    46801 acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag tccgtcgtca
    46861 cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct aaacttaaaa
    46921 ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg atatcgcctt
    46981 ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc aatgtttgtt
    47041 cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc actttgatgc
    47101 caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca taatgatcaa
    47161 taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca ccaaatgcgt
    47221 aggcatgatt taatttatct accgttccat aacctacgat attcgtataa atatctcggt
    47281 ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc atcatcgtat
    47341 cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat agcaaaaccg
    47401 aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat attcgattag
    47461 agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg attccgtatg
    47521 ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga actttttgcc
    47581 atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat ataactgttc
    47641 aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt gtgtaacgat
    47701 tattattttt taaaatttct caaaattctt aactatcata ccataccgtg aaaaagatcg
    47761 aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt ttttccagaa
    47821 aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc acataccttt
    47881 cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc acgacaattt
    47941 cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt ccagctcact
    48001 gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt taatggatag
    48061 atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg acatggtaat
    48121 gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa aactgaaatt
    48181 tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag ttaggacatg
    48241 tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa atagacaaaa
    48301 agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat ttagtcactt
    48361 cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct tgaccttctt
    48421 ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg tcatctccgc
    48481 cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc gttccgtgtt
    48541 ccataaatgt atatcctcca aaaaagttct tgcaagtaac cttgcaagaa cattataccc
    48601 aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt ttaagactga
    48661 tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc atcccaatta
    48721 ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt caaataataa
    48781 gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa tggagaatct
    48841 tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca agcccatcca
    48901 gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga aaaatcacca
    48961 aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc atttggtgcc
    49021 atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg tacagctgtc
    49081 ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt tttttcgcct
    49141 aattctgggt atttctcaat tgctgaattt aaattcgtca cataagtatt gtgatgctta
    49201 tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc atatgcataa
    49261 ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt cttaataatg
    49321 tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc ctagtcgtct
    49381 ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat aaacaacttg
    49441 ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc cacatactca
    49501 ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc gtaagaaagg
    49561 atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt cggtgatttt
    49621 gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca aaaccacgaa
    49681 atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc aaaactaaca
    49741 aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc ccaatatcct
    49801 tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc taccttatta
    49861 aaaattctta tttatcatac tctatatttc aataactttc atctttattt aaaaaaatta
    49921 attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta ataccatatt
    49981 ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt agtagctgat
    50041 cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc caaatatgga
    50101 catgatgaac atcctttacc ccatcgattg cttcaatttc ttgttccatt ttttcatagt
    50161 cgatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc attatcgctt
    50221 ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag tagatatcga
    50281 aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta tcacttaaca
    50341 aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt aataataata
    50401 ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa tgactattta
    50461 tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag gataatatga
    50521 tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag ccatacgtac
    50581 gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa ataattgcca
    50641 atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct gacataatcc
    50701 ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca aatagttttt
    50761 ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt atattggata
    50821 gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagct gtttccttaa
    50881 agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact actagatgag
    50941 gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc aaagacctaa
    51001 aggaggcgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt ttgagtataa
    51061 ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt caattggatg
    51121 ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag cttcaaaccg
    51181 aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt gatccagagg
    51241 gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt agtgtcggga
    51301 tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct gctttgttga
    51361 acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta acaggtaagc
    51421 agctccgttc ttttttaagt gaagcatcta taccactttg gataaaagtc ctgacattta
    51481 tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt accaacattg
    51541 ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat tgtttaaaaa
    51601 cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg gtctttcttc
    51661 cttcttttga cactagcgcc ttcattgctt ttgcaggaat ctttattttt gctcaagctg
    51721 tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt tatttctact
    51781 tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt ttatttgaaa
    51841 aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta tgcgctacac
    51901 ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat gcagatcgtc
    51961 catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat atctttaaaa
    52021 cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg aactcagact
    52081 accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc tcagctggtg
    52141 ttgatcagct accccttgag actttttcaa agaaacatat tttactttca aatggtagtg
    52201 gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg tattctcgtg
    52261 gtctttttca agcacaaaag gcacagagtg acatgaaatg gttaggttca tcggtccatt
    52321 accaatctat ccaagggaaa aaaatactaa tcattggaac tggccatatt ggaaaaatga
    52381 ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca tccggtcatc
    52441 cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt cttcctgaag
    52501 tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc tttgatcagc
    52561 atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt ggtgcttctg
    52621 ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt gcagcattag
    52681 acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg gacaatgtac
    52741 tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta atggctattt
    52801 ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat caagtaaaat
    52861 tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaaaaga gaaggtgaca
    52921 aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg gctcctccag
    52981 gttgggatca taatcaacaa gtaaatcata tcctttaaag aaccattcat ctgcttcctc
    53041 gataaaaaaa agtgtcccat caatttccac tttctttaca ggatgttctg gctgttcaac
    53101 tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt ttccaaaaaa
    53161 acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt gtgcttttgg
    53221 cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt gattcatctt
    53281 atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat cattttcacg
    53341 aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc gtatggcgac
    53401 tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt tgaaaaattt
    53461 aggattggag actcggattt ctgctaaaga atatttatct gacaactcta ggtaatctaa
    53521 aatattcttt gacactaaat tatgcgcaat ccttattccc atgtctcgct cgggatggac
    53581 gacgcgatca gctccgatct tttcaagtac tctggcatga tactcatttt gggcttttgc
    53641 taaaatattt ggtacgccca tttcctttac catcagagta actaagatac tggcttgaat
    53701 atcttctcca atagcgacaa ttacatgatc gaaatttctt atgcctaatg atcgaagtgt
    53761 catttcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat attcattgac
    53821 ccgatcttca ttactatcaa tagccagtac ttcttgacca gcttcgatca gtgttcggca
    53881 gatacttccg ccaaatcgtc caagacctac aattgcaaaa ttttgtttca ccgttttcct
    53941 cctttagtat cttcttttct cagtataaca aagcctttac aaaaacaaaa gcaagcatca
    54001 ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat tatcctcttt
    54061 atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct tgtgtacggt
    54121 cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt ttcagtgtaa
    54181 taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc attaaaattt
    54241 cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga ctcatttttt
    54301 ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc gttgccctta
    54361 tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct gcacctgctt
    54421 caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg attttagctt
    54481 ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct tccatgacta
    54541 agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct tgtcgtccat
    54601 tttcagcttc tccaattact tcaatgtctt cttgaataga taaataaaaa gagacgccta
    54661 agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc tatgcctcct
    54721 tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta atgattttta
    54781 ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa cttcccgttt
    54841 tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa agattattgt
    54901 ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat gtatttgata
    54961 acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt tttacatctt
    55021 ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat aactgttcga
    55081 taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga agcagcgcac
    55141 gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc ttgcgaaaca
    55201 tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc atcattgctg
    55261 caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt ctttctaatt
    55321 ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt acaatttgta
    55381 attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta tcaatatctt
    55441 tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg gattcataat
    55501 tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc ttttgaataa
    55561 gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac aaatggaaga
    55621 taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga ctttgattgc
    55681 tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca atccccgaat
    55741 aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct cgacatctcc
    55801 aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat cactgctgta
    55861 gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga catttcctac
    55921 taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc gtgttctgcc
    55981 tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat tgcccaaatc
    56041 tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat agacgtcgtt
    56101 tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat gtgtttttaa
    56161 ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt tggttaaatc
    56221 tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata cagccatcat
    56281 ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta atatcaattg
    56341 aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat tgaaaatacc
    56401 gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag caaataacag
    56461 cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc tttctccctc
    56521 ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag gactttagat
    56581 gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga gtgtatcatc
    56641 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
    56701 aactaacata tcgccacctg gagtagcaat agttacttca tcatttttac gtttgccaat
    56761 caatgcttgc gcaataggag aatcattgga aattttgcca gaaaacggat ctgcttcagc
    56821 gcttccgaca atcgtgtact cttcttcttc tccatctggt agttcgatga atgtaactgt
    56881 tttaccaatt gatacctcat ctgcatctac accgtcgtta tcgatgattt gagcaaaacg
    56941 aatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttcgctga
    57001 ttcatattca gagttttcgg aaagatctcc gaaaccgcgg gcaattttga ttcgttcaat
    57061 gatttctccg cgtttgactg tttttagttc ttctaattct ttttctaatt tttctttacc
    57121 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
    57181 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttcttc
    57241 aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc ctgtttttct aagaattatt
    57301 tacatatttt tccactaatt ccatatgttc ttcataagtt ttagcgaaat aaacatttcc
    57361 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
    57421 gattgcctct tcgcttggat tgtcaaaagg tccaggtcca tagccggtat tggtatacaa
    57481 attatatggt gaatctacag ctgtatcttc atacgtcact aattctttgt gttcacctaa
    57541 tgcgtataaa acagagatat ctgactgaag tggcatattt gctttgattc ggttaaagaa
    57601 gacttgtgca atgtttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
    57661 tagagttaaa acttcttgta cagtcaaatt tttctgcttg atagctgaat aatagttttg
    57721 catgaccgta ttcgtcttgt tcaccatttc aatcactaga tccttaagcg aagtactcgt
    57781 gtaataatca tatgtggctg ggaataagta accttccaaa cggtattttg tgttctcagc
    57841 tttagatgca ctttccaaca gttctgggaa tttttggtgc aactcgttaa agaaagtctc
    57901 atcattcact aaatctaaaa attctttctt gtctttacca gttaccttcg ctacccgttc
    57961 agcaatctga tcgatatcgt acccttctgg aatggcaatt ttcccatctg caacttttgt
    58021 tggctcagaa gtaccgcctt cttgcaactg tttgccaatc tcgtcaagag tcatattagg
    58081 cgctaactga taatagccag cttgaaagcc agttaaattt ttaaattttg tataataatt
    58141 gaaaacaatc ccgcttttaa taatattgtc cttctccaaa atctcaccaa tctgcttgtt
    58201 agaagagcca ctcgggatct cgacttggac taattggtca tctgatttat ctaaaggctt
    58261 caacccgcta tctacataac gatacacggt aaatccaaga aatccgccaa taatcaataa
    58321 tgtaagagca atcactaaaa tgatcttcct cacaatctta tcttcttttt tacgcagatt
    58381 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tatctttact
    58441 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcaagttc gtaactttat
    58501 tcattataca tgatatttga attggcgtga atgattttat ttaaatattc tatcaaaaat
    58561 gtaagtagta aatccattac tataagatta aaataaatgt tatcaagata caagctattt
    58621 tagctttatt ctttctgtaa aaaagaaagg agactaaata aactattatg aaaaaaggaa
    58681 aacatgctaa aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg tttagcctta
    58741 gtgcgttctc tacaggaact attcttggat ggcaatttta caataatgaa cactctcaga
    58801 tagttgccca tgctcaatca gctcaaaaaa agttgatact gactaccaat gaacaacagc
    58861 tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta tattggctca atttacgtaa
    58921 agaaaaataa tcgcatcatc atggaaaaag gctatggcta tgcgaataaa ctcacgcaac
    58981 aaccaaatga tccttctctt tattatcaga ttggttcggt tcaaaaagca ttgactactc
    59041 ttttgatttt aaaacaagtc gagcgtgggc acctttcttt agatactaag ctggctcaat
    59101 tctatccaca aattgctggc agccagcaaa ttactatccg tgacttgctg tatatgcgct
    59161 caggcttgca ccgaactgct tctccaacca taccgatgac tgatgaagaa gtcgttcaat
    59221 ttgcgattca gcacttaaaa tttacagatt atcagacctc tcattatgat tcattgaact
    59281 ttaccatatt gactggaatt ttgatcaaat taacaaatca accgtatgaa aaattattga
    59341 ccgatgatat catcgaacct ttaggattac agcatacaaa attttatgac caagtcaaaa
    59401 attcatccaa tcatgcacta tcctatcaaa tgtctaaaga ggatgattac tttaaaccac
    59461 tgacagaatc tgaaacagag atccataacg aattagggac tggtaatatc agtatgtccg
    59521 tctatgattt aaatacgctg tttacacaag ttttatctgg aaaactagtt tccaaagact
    59581 tactcttctc attatggcgt gaaaatgcga atggacatcc atatagtggt ggggtatata
    59641 gcggagataa ttatatcctg tcgcaaggaa atatcgactc ttttgataca tctgctatat
    59701 ttagcaaaga ttcacaggat gctatcatca tggaaagcaa tataaaagct gacaaaacta
    59761 tcaaactgcc tactgctgat ctaagaaacc aaatctatga cctgatggaa ggaactacta
    59821 agttggcaaa tagctgatat gatgaaaaga tagcaaatac tacttctttg aatagcattt
    59881 gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg ggacaaatct aaaaatcaat
    59941 ttttgctaca gactttaatt ccaaataaac agaacagaaa gaggtgttct tctttctgtt
    60001 ctgtccaaat tattctaaga tttctcttta ccaacattat ttgacaaagg gcctattaaa
    60061 tctaaaatag tatctttaga ttgctaaaat atgtgctagc aaatcattca tggcttgttc
    60121 ttatttttaa ttttatgcaa taaaaaagaa gaacaattag cgtgattata gaaataaata
    60181 atgagatttt agaaaaaaat gtgtatgaat acctgattgt tactttattt attccacttt
    60241 cactttttac tgtaacaaaa ggacctcctt gttttagtac tgtagcattt tttgaattaa
    60301 cagtaaccgt taatccttta taccggaata caggtaaaac tattgttttg ccttttgaag
    60361 atttaaacgt aaatgtaatt tcatctttat ttggagaaaa gctactataa ataacttgac
    60421 catcttctgt aacttgatgg tttttcaaaa gcaattgatc ttttttatag cctataggta
    60481 cataatcaaa attctcaaat tcttcaagtt tattttccat ttgttcatta gaataagttc
    60541 cttgaaaatt catttctttc accatgttaa ctactgatga ataatttaaa attgaaatag
    60601 ctaatattaa taatagtata aaatttcttc tccttgtata agaaaatttt tccaactgtg
    60661 gagaaataaa tagagatact gaaaaagata tgaacaatgt tgaaaatatc ataaaacgcc
    60721 aaggagcttg aataatattt aaaggagtat tttgtaaggc aaaccatgga aatatttttg
    60781 tacaaagtag aatagatatt atacccatta tataagtgat tttagataag attcctaatt
    60841 ttttaaagtt aaatgttagt ataattaaac aaataagcaa agttaagcca ataccaaatg
    60901 atagtaattc tgaatctata cttccaatca aaaactcttt acccagataa attgctcttt
    60961 tttgtaatat atccacataa ggtctattca gatcttgata caatgtctgc tcaatcatag
    61021 gacctataaa cgctagtgaa agaagtaaag taactatagc agcttttatt aatttaataa
    61081 ttaagaagat ttctattttc tttttccgat aaaattgaat aattaaagca attataatca
    61141 ttaccatagt catagctaca gaaagaacat gagtgtaaat taataaagtc atgctaatcg
    61201 ccaacataac ccaagattct ttttttgagt cgtaaagctt tataaacccg agtaaaataa
    61261 gaggaagtat tgacattgcg agtaattccc ccattgcccc tcgaagaaat atttctacac
    61321 tcctaaaaga agatgttgta tatataatag cgaaaaagga gctagtaaca tgttttttgg
    61381 taatttcata cattacataa tgacatataa atagagtaat gagcgtaaca atataaagaa
    61441 aaaggttata gcctattgct aaattagtaa acttgatcaa taaaaataaa ggatatagag
    61501 ttaaccatgg gtaaaaatta tttacttgca tgccatatga tctaaacgtt tcaaaattaa
    61561 taggggagct aaaaatagat tctaaactta acgcacgatt aatgtgaaat gccgtatcat
    61621 aaaagccaaa tatttccatg tttttaaatg ctggaaataa atataataaa ctaacaaatg
    61681 cataaactat aaatattttt acagaaaaaa tttttttcaa attcatatac cacctcatgt
    61741 ttttttatat tacatatagt atcatttatt ttttttaaac actagattag tcactatata
    61801 aaatgtgatt aattttatta ttaataaaat attttttata ttacaaaagt agtgtttc
//
LOCUS       DACJGB010000009        58376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.009, whole genome shotgun sequence.
ACCESSION   DACJGB010000009 DACJGB010000000
VERSION     DACJGB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.009"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..1199)
                     /locus_tag="IYI34_000598"
     CDS             complement(<1..1199)
                     /locus_tag="IYI34_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558495.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADK"
     gene            1321..1905
                     /locus_tag="IYI34_000599"
     CDS             1321..1905
                     /locus_tag="IYI34_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558496.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1916..2161
                     /locus_tag="IYI34_000600"
     CDS             1916..2161
                     /locus_tag="IYI34_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558497.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            2167..2889
                     /locus_tag="IYI34_000601"
     CDS             2167..2889
                     /locus_tag="IYI34_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558498.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            3020..3763
                     /locus_tag="IYI34_000602"
     CDS             3020..3763
                     /locus_tag="IYI34_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558499.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            3769..4134
                     /locus_tag="IYI34_000603"
     CDS             3769..4134
                     /locus_tag="IYI34_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558500.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            4150..4680
                     /locus_tag="IYI34_000604"
     CDS             4150..4680
                     /locus_tag="IYI34_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558501.1"
                     /translation="MSQNISAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            4842..5210
                     /locus_tag="IYI34_000605"
     CDS             4842..5210
                     /locus_tag="IYI34_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558502.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            5401..5661
                     /locus_tag="IYI34_000606"
     CDS             5401..5661
                     /locus_tag="IYI34_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558503.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            6279..7079
                     /locus_tag="IYI34_000607"
     CDS             6279..7079
                     /locus_tag="IYI34_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558504.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            7116..7298
                     /locus_tag="IYI34_000608"
     CDS             7116..7298
                     /locus_tag="IYI34_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558505.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            7488..7775
                     /locus_tag="IYI34_000609"
     CDS             7488..7775
                     /locus_tag="IYI34_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558506.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            7866..8105
                     /locus_tag="IYI34_000610"
     CDS             7866..8105
                     /locus_tag="IYI34_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558507.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRMFSYYESMRCLNKSRSEIIE
                     SSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            8233..8562
                     /locus_tag="IYI34_000611"
     CDS             8233..8562
                     /locus_tag="IYI34_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558508.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            8615..9446
                     /locus_tag="IYI34_000612"
                     /pseudo
     CDS             8615..9446
                     /locus_tag="IYI34_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9461..9778
                     /locus_tag="IYI34_000613"
     CDS             9461..9778
                     /locus_tag="IYI34_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558509.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            9781..10013
                     /locus_tag="IYI34_000614"
                     /pseudo
     CDS             9781..10013
                     /locus_tag="IYI34_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10128..10604)
                     /locus_tag="IYI34_000615"
     CDS             complement(10128..10604)
                     /locus_tag="IYI34_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558510.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            10730..12061
                     /locus_tag="IYI34_000616"
     CDS             10730..12061
                     /locus_tag="IYI34_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAR0558511.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            12086..12613
                     /locus_tag="IYI34_000617"
     CDS             12086..12613
                     /locus_tag="IYI34_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558512.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            12600..13163
                     /locus_tag="IYI34_000618"
     CDS             12600..13163
                     /locus_tag="IYI34_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558513.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            13581..14318
                     /locus_tag="IYI34_000619"
     CDS             13581..14318
                     /locus_tag="IYI34_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558514.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(14335..14751)
                     /locus_tag="IYI34_000620"
     CDS             complement(14335..14751)
                     /locus_tag="IYI34_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558515.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            15144..16391
                     /locus_tag="IYI34_000621"
     CDS             15144..16391
                     /locus_tag="IYI34_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558516.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSNPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            16661..19051
                     /locus_tag="IYI34_000622"
     CDS             16661..19051
                     /locus_tag="IYI34_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAR0558517.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            19100..20935
                     /locus_tag="IYI34_000623"
     CDS             19100..20935
                     /locus_tag="IYI34_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAR0558518.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            21010..21867
                     /locus_tag="IYI34_000624"
     CDS             21010..21867
                     /locus_tag="IYI34_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR0558519.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNVNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            21857..22936
                     /locus_tag="IYI34_000625"
     CDS             21857..22936
                     /locus_tag="IYI34_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR0558520.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSESVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLKVLILLIKEFRKA"
     gene            23275..24075
                     /locus_tag="IYI34_000626"
     CDS             23275..24075
                     /locus_tag="IYI34_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558521.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            24163..24969
                     /locus_tag="IYI34_000627"
     CDS             24163..24969
                     /locus_tag="IYI34_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558522.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            25201..26070
                     /locus_tag="IYI34_000628"
     CDS             25201..26070
                     /locus_tag="IYI34_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAR0558523.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            26090..26437
                     /locus_tag="IYI34_000629"
     CDS             26090..26437
                     /locus_tag="IYI34_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558524.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            26450..27712
                     /locus_tag="IYI34_000630"
     CDS             26450..27712
                     /locus_tag="IYI34_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558525.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            27792..29168
                     /locus_tag="IYI34_000631"
     CDS             27792..29168
                     /locus_tag="IYI34_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAR0558526.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            29165..29794
                     /locus_tag="IYI34_000632"
     CDS             29165..29794
                     /locus_tag="IYI34_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558527.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            29806..30276
                     /locus_tag="IYI34_000633"
     CDS             29806..30276
                     /locus_tag="IYI34_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558528.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            30273..30755
                     /locus_tag="IYI34_000634"
     CDS             30273..30755
                     /locus_tag="IYI34_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558529.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            30752..31414
                     /locus_tag="IYI34_000635"
     CDS             30752..31414
                     /locus_tag="IYI34_000635"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_002351343.1,
                     RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAR0558530.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            31401..31769
                     /locus_tag="IYI34_000636"
     CDS             31401..31769
                     /locus_tag="IYI34_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAR0558531.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            31850..32296
                     /locus_tag="IYI34_000637"
     CDS             31850..32296
                     /locus_tag="IYI34_000637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558532.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRITEQAIEKQKDKEKKEIMSIYLSSGASFFHEID
                     SMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            32948..33001
                     /locus_tag="IYI34_000638"
                     /pseudo
     CDS             32948..33001
                     /locus_tag="IYI34_000638"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33005..33604
                     /locus_tag="IYI34_000639"
     CDS             33005..33604
                     /locus_tag="IYI34_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAR0558533.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            33620..34096
                     /locus_tag="IYI34_000640"
     CDS             33620..34096
                     /locus_tag="IYI34_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558534.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            34113..35015
                     /locus_tag="IYI34_000641"
     CDS             34113..35015
                     /locus_tag="IYI34_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558535.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            35088..35279
                     /locus_tag="IYI34_000642"
     CDS             35088..35279
                     /locus_tag="IYI34_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558536.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            35443..36030
                     /locus_tag="IYI34_000643"
     CDS             35443..36030
                     /locus_tag="IYI34_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558537.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            36030..36929
                     /locus_tag="IYI34_000644"
     CDS             36030..36929
                     /locus_tag="IYI34_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAR0558538.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            36929..37843
                     /locus_tag="IYI34_000645"
     CDS             36929..37843
                     /locus_tag="IYI34_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558539.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            37895..38110
                     /locus_tag="IYI34_000646"
     CDS             37895..38110
                     /locus_tag="IYI34_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558540.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
     gene            38295..38549
                     /locus_tag="IYI34_000647"
     CDS             38295..38549
                     /locus_tag="IYI34_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAR0558541.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            38728..39771
                     /locus_tag="IYI34_000648"
     CDS             38728..39771
                     /locus_tag="IYI34_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAR0558542.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            39944..40168
                     /locus_tag="IYI34_000649"
     CDS             39944..40168
                     /locus_tag="IYI34_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558543.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            40171..40428
                     /locus_tag="IYI34_000650"
     CDS             40171..40428
                     /locus_tag="IYI34_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558544.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            40530..40883
                     /locus_tag="IYI34_000651"
     CDS             40530..40883
                     /locus_tag="IYI34_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558545.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            40936..41730
                     /locus_tag="IYI34_000652"
     CDS             40936..41730
                     /locus_tag="IYI34_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558546.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            41759..42877
                     /locus_tag="IYI34_000653"
     CDS             41759..42877
                     /locus_tag="IYI34_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558547.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            42910..43230
                     /locus_tag="IYI34_000654"
     CDS             42910..43230
                     /locus_tag="IYI34_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558548.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            43485..44174
                     /locus_tag="IYI34_000655"
     CDS             43485..44174
                     /locus_tag="IYI34_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAR0558549.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            44211..44378
                     /locus_tag="IYI34_000656"
     CDS             44211..44378
                     /locus_tag="IYI34_000656"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558550.1"
                     /translation="MVNEVETLLASFFENGHVNDEWRKEEPKRVNAYIARLKRFIDKY
                     KENSDKFSKDK"
     gene            44398..46026
                     /locus_tag="IYI34_000657"
     CDS             44398..46026
                     /locus_tag="IYI34_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558551.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            46077..46448
                     /locus_tag="IYI34_000658"
     CDS             46077..46448
                     /locus_tag="IYI34_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558552.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            46533..46733
                     /locus_tag="IYI34_000659"
     CDS             46533..46733
                     /locus_tag="IYI34_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558553.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            46867..48093
                     /locus_tag="IYI34_000660"
     CDS             46867..48093
                     /locus_tag="IYI34_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558554.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            48690..49085
                     /locus_tag="IYI34_000661"
                     /pseudo
     CDS             48690..49085
                     /locus_tag="IYI34_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            49163..49675
                     /locus_tag="IYI34_000662"
     CDS             49163..49675
                     /locus_tag="IYI34_000662"
                     /inference="COORDINATES: protein motif:HMM:NF024954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0558555.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLLHRLGLSWTRPTYTLKKADSKKQQVFLE
                     ETFPSLKKNC"
     gene            49933..51153
                     /locus_tag="IYI34_000663"
                     /pseudo
     CDS             49933..51153
                     /locus_tag="IYI34_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352449.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
     gene            51146..51493
                     /locus_tag="IYI34_000664"
     CDS             51146..51493
                     /locus_tag="IYI34_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558556.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            51844..52665
                     /locus_tag="IYI34_000665"
     CDS             51844..52665
                     /locus_tag="IYI34_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAR0558557.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            52685..53245
                     /locus_tag="IYI34_000666"
     CDS             52685..53245
                     /locus_tag="IYI34_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAR0558558.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            54134..54901
                     /locus_tag="IYI34_000667"
     CDS             54134..54901
                     /locus_tag="IYI34_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAR0558559.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            54927..55472
                     /locus_tag="IYI34_000668"
     CDS             54927..55472
                     /locus_tag="IYI34_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558560.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            55473..56039
                     /locus_tag="IYI34_000669"
     CDS             55473..56039
                     /locus_tag="IYI34_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558561.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            56054..56302
                     /locus_tag="IYI34_000670"
     CDS             56054..56302
                     /locus_tag="IYI34_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558562.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            56321..56560
                     /locus_tag="IYI34_000671"
     CDS             56321..56560
                     /locus_tag="IYI34_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558563.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            56577..57161
                     /locus_tag="IYI34_000672"
     CDS             56577..57161
                     /locus_tag="IYI34_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558564.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            57268..57549
                     /locus_tag="IYI34_000673"
     CDS             57268..57549
                     /locus_tag="IYI34_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAR0558565.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            57539..57880
                     /locus_tag="IYI34_000674"
     CDS             57539..57880
                     /locus_tag="IYI34_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR0558566.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
BASE COUNT        22094 a         7771 c        11679 g        16832 t
ORIGIN      
        1 tccttatctg ctaacctgaa tgaactagca tcggtaagat gttcgatcat aaaatctatg
       61 aacatctctt tttcaccaaa atataaatat tgattatata ttgtttctaa tgttgaagat
      121 ggtagcgaag aaactctcat tttttgctgg agtataggat caactagact atttaagtga
      181 ttttgtaatt cttcattgtt gtttcctaga actgcaaggg aacgtaaacg gtagtaatat
      241 tctatttctg ttagtacttg ttgtttcggt ttaaaatcat ttattgatat ttgtaaattt
      301 tgaattgtct cgtctgaaaa atcatgttgt aattctggat gggtagctat aaaagtaagt
      361 aaaaagaacc cttttgtata cgtgatctgt tgattttcac caagtgattt attaatttcc
      421 tctcttgatt cagctgtgaa cctatacttt gcaatttctt tttttagaat atttaaatag
      481 aattctttat tatcaaaaat tagggcatta agtagtctga aagtactttc cctatttcta
      541 tctgcttcct catttccata agataattga aaaacaaact taaaggctgt cttacatagg
      601 ttttctttgg ctttttcgct tagtttattt agatagctat tttttagtgc agagtgaaat
      661 tcttcaggtg taacagacat agcatgtatg cgacttgcat attgtataat ttcgatcgtt
      721 actttttttc ctaatattgg attttttagg aatataattt cataggactt ccgtataaca
      781 tcctgacaag tttcttttgt tatatctctt aattcccaag tattaccgtt gtaagtgact
      841 acaggatgag ctgaataatt tctttgatcc ttaatatatt ttaaatcctc atattcatag
      901 ctatcaatta gctcagttga actataagct ttttctagta gttccatttc ccattttgga
      961 gagtttggat cactttttct tagtctagct atatcttcta aaattttctt agaagtatta
     1021 tcctcgtaaa tatttgctaa atcagataat ttatcaatta aatctaaaat aagcgcactg
     1081 taattgacag ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct
     1141 aataatggaa taattctagg atttttaact ttttttattt catcatctaa tataaacata
     1201 taaatctcct ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga
     1261 catatttatg ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa
     1321 atggatttga aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg
     1381 gctgatattt ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat
     1441 ttaatcgggt acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt
     1501 gctaacaatc aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat
     1561 gtgatccatg aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca
     1621 atgacaacgg atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg
     1681 cgtgatggtc aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa
     1741 atttcttttt gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt
     1801 aaaacagcct ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac
     1861 ccatataatt ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa
     1921 aaaaattata aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat
     1981 gtattttact gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct
     2041 attatttagt ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt
     2101 gttttattgg atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg
     2161 aataaaatga aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataact
     2221 cttacagcat gtggtacaac taatagtgct gataataaca aagacacaaa gtctagtagt
     2281 acggtggtta ctaaaaacag ctctacatcg gcatcaacaa caatttcttc aatgactgaa
     2341 aataatgctg aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggaagaa
     2401 gtttataccg actttattaa tacagtaaaa aatgatgaag attatgggaa tgatccattg
     2461 caaaataaaa atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca
     2521 gaatcaaaaa tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat
     2581 gatattacca taaataaacc agcttacgag tatcacgcag ttgctgtaga tattactgat
     2641 ggtgtacctg agtggataca aaacattcct ggtctggctg atgagaacaa tcaatatcat
     2701 acgccaacag ttgcagtagt aacaccaaag aaagttaatg gcacactata ctgggatgta
     2761 atgggaatga caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg
     2821 gatttgggag catataaatt atatcaacag tcagaacctg ttatggacga tcaatccaaa
     2881 gttaaatagt tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa
     2941 tgatttctga aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga
     3001 ttataaagga gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa
     3061 gaatcaggtt ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact
     3121 taccggttac aataacggtg caaatgggaa gtggttaaag gcaaaaagta ttcgtgaact
     3181 taaaactgaa gtagaaaaaa taagacttgg gttaacaagg gaagaaataa gtagactgag
     3241 tgaaaattct tcttttgtaa tccaaaaaga aaaggagaaa catctacttc atcgggaact
     3301 aataattgaa aaatactacg atgttgattt acctgatcag ttaagaatta ttgacgaatt
     3361 ttcagagaaa aagtggaaat ctgaaatata cccaagctta attcaataca aggcatcagg
     3421 aaagcccatg tataactaca caggcttttt aaactacaca caatataaat atgcctttcg
     3481 caaattaaaa ggcattaaac aagctgacat tgccctgtat gagatgaaaa aaatggatag
     3541 attgaaattg attcaagagt tagtgcaaaa tacatggaat caagcgattg tcgaactaga
     3601 aaaagaaaat gggaaaattt taactaatga atatctgatc gtaaatgaaa ttgaagaatt
     3661 aattggttta ttttcatgtg aaaatggtga agtcttcttg aagtcatcac caatctattc
     3721 aattttggac agtccgtatc agtttataca ggtaaggaga tgagttacat gtctagcttg
     3781 aaagatgact atatccaagc aaaacaagag tatgaaaaaa tacagatgtt aaaatctgag
     3841 aaagtcgaat tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct
     3901 aaaactttag aggaatatga tcagcagttt aatcagacac atcacaatat cattttgctt
     3961 gttgatcagg caaagagaag acttgaaaaa gcggaatatg aactattaga tcaaggaatt
     4021 atatgcttgg ataaagggat tcgaagatat tatagtgcag agttaaaaaa gcaaaatgta
     4081 gacagacatg cgttaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat
     4141 agaaagtaaa tgtcacaaaa cattagtgct gtaaaacaat taatagaaaa agatttagag
     4201 acgacagttc agaggttagc cgaacgaatc cattttgcaa attatcaaac catctctaat
     4261 tggtcaagca ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga
     4321 aaagaatatg gtgttgatcc gataaaaatt ttcgaaaaga ccatgattta ttactatcat
     4381 ggaacaccta acgaagtgtt ggaacatctt ttggaagaag cagaagaaaa atcatcaata
     4441 ttagagatat cacaaaatga caaagctatt caagtgaaat gtgttgatcc aacaggaaag
     4501 attattgttg gatttgatat tgaaagaaaa tctaaaatag attttattac tcaattaaca
     4561 aagataaata atttaaatat tttaacactt agaccagatc atgaacagtt gtttatgagt
     4621 tttaaaaaac aattgaaaga aatacaaaaa agatataaca aggtgttttc taatgcgtga
     4681 aaagctaaaa gaaatcagag tgttttggat aattaacaaa gaatttatta tcttgtatac
     4741 acttggattt atcagcatga tcttgttttg gtggttatat ttaaaaaagt aatttaaaag
     4801 tgtcaaggag atttttatcc agagaaggat gtggaatttg catgtggaat aataaattaa
     4861 aaacaggctt gttattaata gttatttcct gcgctatgat ggttggcatg ataatacaaa
     4921 gggaacaatc ttattttgaa gtttctgcta acaatgtaat agaaagatgc cattatggtc
     4981 aacatttttg gactggggaa gtcagagaaa atatagatcg tgagtatgtt caaagaatcg
     5041 tttgggatgc atattctatc aaagattatc ctaaaagttt aacgagtaga ttattttaca
     5101 gcgaaaaaga taatcaaaaa ctctcgaatt tgatgatgaa aaaagtaagg aaacttgcac
     5161 aaaactattt ggaagaaaaa gaaggtaata gtccaattga caaaaattga tgacactgca
     5221 aaaaaatata gagttcgaat tagctttttt cggagcattg ttttttaggt gaactatatt
     5281 tcatcactaa aatcagagat aactaacttg aaacatggtc agatgtgtta cttgaagtct
     5341 ctgaataatc aatgttatct agtagaatta agcaactaaa aaagaaagaa gtaagaaaat
     5401 atggcatacg aaaatttaca attagtagtc gcagaagtta gtggggatat ataccttaca
     5461 aaagtaaaag atggaattat ggacacgaat tataggagag ttgctacaga tgatgtgcta
     5521 agctcatcga cagaatggtt tcgaaaaaat gaagaatctg ctgttcactt tgaaggaatt
     5581 gatggaaaca ttcatagttt gttctataca aaagacaaag aaaaagcaaa aaaaattctt
     5641 gatattttga aaggaaggta gttaattaac caatgatata agagaatgtg agaccaagta
     5701 aatacttggt ctctataatt ttagccacta gtcaattaaa tagctaaaca attttaataa
     5761 taataaaata tgactgcttc ataattaaga agtgcagaat ttttctaaga acgatatttc
     5821 cttgttgaaa tgagtattgc taacaatttg atcacgaaca gcatcgttac aaatgttgta
     5881 tcaatatttg atagattaca tattctatta aaaaagaaaa gaaaattatt gatattatac
     5941 acattagtgt gtataataaa agagagggag tgattagaac tttgagcaat tcactaattg
     6001 tttaataata gaacgattcc taagaagttc attgttcatt ggcaaattgc tttatcagat
     6061 tgaaagttgg cgaataaggt ttgtagaata acaccttttc aagcaaagcg atttttgaaa
     6121 gctatgaaat ttgtttaata aaaaaattca ccagataatc tgatagagac taacccaatt
     6181 actaacagtt gtaattggtg ttagcttaca aattaattat tgtgtttagt caattacaaa
     6241 ttgtgaaacg gcactaataa ttggaaagga aaaatgaaat gtctaaagat ttacgagagc
     6301 ttaaattgcg aaaaaagcaa tataatcaat atttttctag tttgttgaat aaacatattg
     6361 aagaaggact tgatcatgat tttggaaaag atgaatattg tttatattgc gaacgttaca
     6421 atgaaaaaat gtctgaatat ctacagctga aaaaagagat acagaaatta gagaaaaaag
     6481 tacatgcttg gaagtataaa gattccaggt ctggttatgt tgaatctcaa aataagccaa
     6541 gaaaaaagaa gcaagcgtat gttctaaaga tggtaaatgg ggaagagtta attgtattag
     6601 cgtacagtga agaagaggtt cgagaactcg ttgaaacacc tgtcaggaaa aaggttgaaa
     6661 caataaatga gatagacgac agccaataca atagcatcta tattgaaaca agtcatggtg
     6721 gatataagaa attagcaact ttccttagaa gttctaaagt tgggataatt tctaaaaaag
     6781 acataagtgc tgttaccgat agtttttgta ctttatctgt tcctaaagct tatttccaat
     6841 acgatagtaa aagatttgaa cgcttagaga aattctgtag accgtatact gtattgaaaa
     6901 ctaaaaaatt taataaaact aaaagagttt cgatacagtt accgactggt caaatctcat
     6961 taggtgaaaa agactatctg ataaaagtag gaaaagagtt aattataaaa gtaccggaac
     7021 acttatttaa agaaaatttt gagatacgtg aaaccttggt atggaaagaa gaaaaatagt
     7081 ttggaagaca tagaatggta taaggggaaa ttgaaatggc agtaaataaa atattgcagg
     7141 tattaatcat agacgaattt ggcggaggag aatggtttga tgtgcagtcc atagactttg
     7201 aagaagaagt cgtagttata gaaaatattt acggcgaagt atactcatat tcgcttgaag
     7261 gtgagaatgt tgaggatcta cggttgattg gtgagtagaa aaaagcagaa atatcaaaat
     7321 ggagataaaa attaatagca tgtaaagata gtcatgctta catgctttat ccaaaatggt
     7381 atgcagacaa aaatatagct gaaataaacg attatctaac tagtataaag gaacgcaatg
     7441 caattaatta acaatagacg aaaaatgaaa ttaaaggaga taggaaaatg gtagaagttg
     7501 aatttggatc aaaagaatgg tttgaagagt taaactcaat gattaagttc ccagaaaagt
     7561 ttacttttta ctacaataaa agagacaata tcattcaagt agctttactt gaaactaagg
     7621 gaagattaga aaatactcga tggagtatga taaaaccatc ggcacttaga tttcatccta
     7681 aagacttgac agcggttgta gcggtaaaag aggttcaaga ctcacaagaa tattatgaag
     7741 tagtaaaaaa tctgaaggat atttggtgta aataattcta aattgaccag ttatttggtc
     7801 aaaaatagta tgtgattatt tcatccaatg tggcatcata attggtatgg aaggggataa
     7861 attatttgaa agaaatagaa ggtattaaga aaagaaatca ggaaagattg gaactgttaa
     7921 atgaaaatag aaatagaatg tttagttatt atgaatctat gaggtgttta aataaatcta
     7981 gaagtgaaat tatagaatca tcaaagtata aacaattgat ggaagagaga gtttctctca
     8041 tccaagaaat agaaacagag tctatttttc cagatcattc tcctaaagtc cgattttata
     8101 attaaacatt aacaaatgaa acgatgctaa ataggcatcg ttttttatct atttataaaa
     8161 ttaatttatt tacaaaatgt gtatcggtat gtatggtgaa tttgtagata agttgagaaa
     8221 gggtgatctt aaatgaaatt agaaggtagc ttacaaaata gaatgatgga aggcagtaca
     8281 acaaacaagg agattgtagt tggaatggga gcaaccgagc ttctatatac cgacagaaat
     8341 ccttatacag taattgaagt aaaaagcaaa aacagaattt tggttcaagc ggatggagca
     8401 atcaataaag ctacttttcc agatcaagag tgggaatata gcagaaatcc tgaaggtcaa
     8461 atattggagt tagttaagac aaaaaatgga tggaaagtgc ttaaagaaga tattcatttt
     8521 tatataggtg atagagaaaa atactacgat cctagttttt aaacaacctg aagatgagcg
     8581 aggttttatc ctcgctcaga aaggagtatg gaggatgttc agtcaagaag aaatcatcag
     8641 caaatcgcaa ccggataaaa atggaactat aatcatgaag aaaaaaatcc cttgctatcg
     8701 ttgtagcgga tcaggtcagt acaagtttat gtctatgggg aaaattacct atggcacatg
     8761 ttttaagtgc gatggtgatg gatttcttgt tgtaactgaa aaatccttta cgccagaata
     8821 tagaactaag ctaaatgttt taaataaaaa aagagctgat cgaaaaataa ggatcgcaag
     8881 agagaaagaa tctcagaaaa attttgatcg ttatggcttt gaaaagggtt atatttacgt
     8941 ggttatagag aaaaatagct atacgatcaa agatgaactg catcatcaag gagctactta
     9001 tgatccttta ttaggttggt ttttttaagg aaaaacaaga acaatacaaa actcaaaaag
     9061 tttttgtaga agaattttct gtaatcaatg atgaaggaat tattgagttg aagctagaag
     9121 aatacagcaa aagacaacag gagattttaa gtgcgaagcg tagagaagag ggtgagttta
     9181 ttggtacaat cgctcaacga ttggaattgg atttgaaact taccaatacc gtcaccatag
     9241 atacacaatg gggcaatgtt tacctgcatt tatttgaaga tcaaaaccat aattcattga
     9301 tttggaagac aaataaattt ctttattatg atgaatggga tgttggagaa ataagaaaag
     9361 ttaaggcgac aataaaagcg catgactact acagagatac caagcagact tggattaaaa
     9421 atgttaaaat tcaaaaaaat gagtaagaag ggataaaagt atgaaaagag aagaatacaa
     9481 acaaagatta aacgaactgc ttgaagagga cgaaacatta actcatggtt cgccggatga
     9541 aattctatac atgattgata atatggtaat ttttggtgga tatgaacttg gtaataggtc
     9601 agttgatcat aatattttag aatttgatga tgtatcatgg gaagaaattc ttgattgggg
     9661 aattttggca gtacctgaaa ctaaaaccta tattagtgac actatggttc ctttttttga
     9721 ggaattggat tataaaagac ttcccaaaaa tgaaaatcat atactgggag gaaactaaca
     9781 atgttaagtg acttacttat tttactgatc tatctaggtg gaactgaaat tattgcgtat
     9841 tttttgcttg taattggtga atatggtatt tccagtgcaa tcactcaaag gtttagatta
     9901 ccagtaagtg aactaaaaaa atgatgagat tatcagcata tcttttaatg tttttgaatg
     9961 tggtcatgtt aatctttgta gtaatcggtg tgactttcaa tttaacggaa tagagggaaa
    10021 tctataatgg aaaaaagaga aaagaatgaa catatttttt gagtcaatat tgttgatggt
    10081 ttttgcgatc agcgcttacc agaaatctaa aaaagagtaa tgactgttta gtcatcactc
    10141 tttttttgta gggaaattga aatatgtgga gcttgtattg gtttagttag gattgcttgg
    10201 ctataaggag ctagttcttt atcaaaacca tcaaagtagg ttttcagtag cgatttaact
    10261 gatcgctcat cttgtttgat ttggataatt tcagcatcca aatcggttgt gaggtcagga
    10321 gcttttgaag cgattacctg atttttttta tcttccaact ttcttaggct gtttaacaag
    10381 ttgtaaaaat aatcattttg actgttcatt ccaacgtgag atgcaatttt aacagaagtt
    10441 tcatccgtat tggcatcaag tatttcatca aaagaactca ttggaatatc aagtagtaac
    10501 gaggatgttt cctttacagc atttttagtt tttccatctt ttttgctctt aacttgagat
    10561 atttttggat ctagtttagg taaatcactt gttttatcag tcatttatta gcttttcctt
    10621 tctattaatt tatgttatta tattatcata ataacataaa aaagaacact ttttgggggc
    10681 gtagttattg aaaaaatcta agagaaagat tgccacgttg cttacgttaa tggtgcagtt
    10741 tacagggatt gcaatgagtg taatcacaat aaatccgcaa gtgttacatg ctgatgggat
    10801 agctacttat gatgcacaat ttaactgtac tactgaaggt agtgggtcag gaacagatag
    10861 cgatggaaac gatactggaa gtggcgctgt aacaggtgat gtagccaaaa atattgaaga
    10921 tatttacaaa tacatgaatg gagaatatgg cttatcaggt gctagtattg caggtatttt
    10981 aggtaactgg aaacaagaat caggtattga tcctcgaaca gtagaagggt attacggcga
    11041 tgggcagaga gaagttgact tcatcaaaca atttactgat ggtctaatgg gtattggcta
    11101 tggtcaatgg acagccgggc gacatacagc attagttgat tggtctaaga aaaataatgg
    11161 cggttcttgg tggactacag aagcccaatt ggactttatg ttcaaaggtg atgggggatt
    11221 cgtaggaata ttaaaagatt atgctttaaa tgcttctgat gatgtagtag aaaatgcagt
    11281 aacttttcat aaaaactggg aaatttcagc tgattccgaa tcagctgtaa tagctgtacg
    11341 tggtgaaaat gcaaaagaaa tctggaagta catgaaaagc aagggtatgg acggtgcaaa
    11401 agatgaaagt aagatcaata aaatatccgg aggttcaagt accggagatg gatcgaataa
    11461 ttcatcaagt tcagcatcag gaacagtaga agatgcctgt gcaactacgg atgatgctaa
    11521 ttctggttct ggatcaattg ggaaagaagg aaaaattaac ggtaaaaaag gtcaagtaat
    11581 agcaaatatg acttatgaag aagcattaaa aaaatatggg aaagcaatca cgctaccaaa
    11641 atttaaagaa ccagactggt caacatcacc ttttcaaggt gaattacaag gacaatgtac
    11701 tgaattaaca tgggcttata tgagtcaatt atatgatgga aatcaaccaa ctttgggaaa
    11761 tggcggttac gtttgggaaa gctacaaatc ggcaggagca aaggtgacga acaaaccaat
    11821 tgtaggttat gcgtttagtg ctttagatgg atggttatgg gctgttggat cagcaggaca
    11881 tacaggtgta gttatagcgg tgtttgacga tggatcattt ttgtgcgcta atttcaatgt
    11941 tccacctacc caagcaccaa caagaggtgt ttcagtaact ttgattgatg gtgtggatgg
    12001 gtcagataat gtgcatttct ttagcggtgt aggcaaagct aaatttgaaa ttgaaaaata
    12061 aaaatttgaa tggagggcga cagagatgaa tagtaaaacg aaagatgcaa ttatcatttc
    12121 tttgctttct ggtatgctat tagcaatgct tgttggaatt ctatatttca ataagcagaa
    12181 tcaagacata gaagctgaaa agcacagtgt gcaaattgaa aatgaattga ccttctttaa
    12241 agaggttaaa ccaaatgaag tggatggatt gcaaagagat ttagaaaatg atattcaaga
    12301 gatgtttaaa ggatcagacc gaagcaaatt tgcgaatcag tcaggtgaag aatttttgcg
    12361 aaagttattt attgtgacag gtaagccacc agttactaac gagagtagcg agcaagaaaa
    12421 aatagaatac tataaacctt ttacttttaa gctaacaaac tttggaatta agacatctag
    12481 tagtgggttg tatgaagcat tgactgattt cgaagttcaa tacaacggaa aaaaagtgta
    12541 tgataccaca ttaaccatca cactaaataa tgataagaaa gtgactgggg gtgcttttta
    12601 tgcaaaatca tgaaattact actgctaaaa aggcgatcgg aatactttcc ataattttga
    12661 tcctttgttg tgtggtacta tttcaaaaga aaaatgctta tgataataat gtcaaagaat
    12721 tggaagacct taaaagtaaa caaatcgaag tagtcaagaa taaccagaaa gtgaaagcta
    12781 tgcaagaagc ccaatacaaa gaattgggaa atgctgaaat caaggaacaa agtaggaaat
    12841 tctttaattt attttacaac tggaattcat gggaaaaata tacgaagaat atgaaggaaa
    12901 tacaaaaaga atttcctcag atagtaaagt ctagtcctgt tgatatctct ggcacaatgg
    12961 taggtactgg taatagtcca atatctagct atactgctga ttactataca acaaatcaaa
    13021 aaggtaaaat ggttgaattg attacacaga caagaaatac tccaacaaca gcaacaacaa
    13081 caatttggcg tggagtaaca agcataggag aaaatcaatt gttttacttg gaggatttag
    13141 atagctatgt aagagcagag taaaataaaa aaagagctaa cggtaccaac gttagctctt
    13201 tgtgctgacg atgataacaa aacctaccga aaaaggattg tttcatcaca aacacaaaca
    13261 gaaaagaatt gataaaaaac ccgaacctca gaatttttat taatcaattc attttgatta
    13321 taataaattg agctgaacat tacaatacgt atatgttgta aaacgtaaaa aaagtcaaaa
    13381 gtaaatactt tcttaaaaag tcaaataaaa ctattaaaga caatatatat ggtttatttg
    13441 acataagaaa atggtctaga tgataattag aatatagaat tgtagactta aataaatcat
    13501 aaaaaacctt ctatgataag ttatagctct taattttaag cagttttaga tataagaaag
    13561 taaagtgggg gagaataata atggcagatc gtaaattaga aaagttatta gaagaaacgt
    13621 gggatccaaa agaatttagt gaatttttta tggagaattt tgaaactgac ttagcggtga
    13681 ttgttaaaga tgctcttaga gagcaagggt atcctgaaac tgcaaattat ataaacatta
    13741 attttacttt gtatactgaa aatagaggaa cttgggattt ttgggctaac ttagcaaata
    13801 aggagttaag tgataaatca gatacaggaa ttcgtaactt ttttgaatca aacagagatg
    13861 attatatgta cgctaacaat caaaataaat tgaactttag agtagagttt gatgaaacac
    13921 ctgaagaatt tatagaaaga caaccgccta aagaaaatgt agcaaaggta ttggaagatc
    13981 gttggaattc agatgaaata gtttcaacaa ttagcgaaca agacggacaa tatgaaccgc
    14041 tagtagaagc ggttagagaa gagctaagac tgaataagtt tcctgatgtt caaaatattg
    14101 atgtttctca aattgaaata aacgtcaata tcaccaatag acttgattat gatagttggg
    14161 cagatatagc attagaaaaa tatatttact caacgttaaa agagtttatc gaaaatagaa
    14221 tgaatatcat gtatctacag catccacagt atttaaactt tggtgttgaa atagctacac
    14281 ctcttgaaga atggaaaatg gagcaagggc ttgactagcc tttgctccat ttttttattt
    14341 tacaaatgaa tacagttctt cgtctttacc aagaatgatt gtttgcccaa ctttgagatt
    14401 atcaggtgat ccttcagcgg tttgtaagga gaacgttcca ttgaacttat cggataccgt
    14461 tataggtagc tttaattctg atccttcctc atctttgaga attgctgttt tccgttcatc
    14521 aaaataaaca gtctttatta gataattttt agtagtggtt tcatcagatt tgtctttgtt
    14581 tttagtagta ctaatttctt tagaattaga tgtattttct atgactattt cgtttttggt
    14641 atttgcgctt gtatttattt gattgaaaat tttaatcgac aagatcccac taagaatagc
    14701 caacatagaa acaagtgtaa ttaatactga taatttcctt tttttgttca tttcttctta
    14761 acttcctttt ttgttttgat tagttagata ttccttatct aataagcctt tttaggctat
    14821 taaatgaaga taaaccccaa aaaattattg caataatgat agtgcaatgg tactattatt
    14881 ataaaataaa ttttgcgaaa ggggtaggtc taaatcgcaa ataaaaagaa attgaaccta
    14941 gacaaataca acttgtatac cgcaagggaa gcaagtctaa ttttgggtaa aggtagaaat
    15001 tatgttacaa acataatcaa ctattatcca aatcgtttgc ctaaaggttc gttcaagaaa
    15061 gtgggtgaca aaacctatat tataacggaa gaaggaatag aagcattgcg atcacaaaca
    15121 gaaaataaaa acaaaaagaa ggaatgaata tgaagaaaaa ttcgcttgct atggttttat
    15181 ttgcatcagc tgtacttgcg gggttatcag taccagtaac ctcacatgct gatgaatgga
    15241 ctgcaaacac accggatcaa atccaaatta caacaggaca atcaagttat gcgttcaaaa
    15301 aaggagatac tatttggtca actgtagaga agcttaaaag cttaggttac aagactactg
    15361 ttgctgagtt tgccgaattg aacaatgttg atttggcaaa aggagaacaa cggactatcc
    15421 cgattggacg aactttcaac atcaataaag atacattaac agttaaggaa gcagatggcg
    15481 aagtagttag tcaaactgtt caaaaagata aacctgatcc tgttgaaaat acggagcaag
    15541 agaacaaaaa atccgagact acaaaagaat cggaaccaac agcaaacact tctgataaga
    15601 cagaaacaag tgcttcaagt gttgatggag caagttcatc taatacaaat aattctacaa
    15661 gtgagaaaga agcatcacag ggaacaagtg aaagtagcac tacggaagct gatactggaa
    15721 catcaacaaa taactcacaa tcaacatcaa atgatgcaaa tgaatcaacc agtacatctt
    15781 ctagtgatca atcacaaaca ggttcaagtg atgaaactga cagttctact tcaccaagta
    15841 accccgaaca accaactaat gttgtgctag gcgttccatt tattagtcaa ggtaacacaa
    15901 tgctttgcga aggaacatct ttgctagaag cgttacatta taaaggtgtt gcaacaaatc
    15961 aagatttaat gacttttgtt aaatcaatgc cactatctcc tgataataat ccaaataatg
    16021 gtttttctgg tgaatggaga cacaatgtgg atggaactta tcaaggtatg aatccggctc
    16081 ctgtagtttc atggggtgtt gctaatggcg gaaatgttgt agatatttct ggttcatcgg
    16141 tacaaggatt aaaagatgag attgtaaatg gaaatcctgt agtagcgtgg gtaacttatg
    16201 cctttgaacc tgctcaacat aaatcaatgt tttggggcga tgctatttgg aatcgacatg
    16261 tagtagccgt tgatggattt aaagacggtt tttaccatgt tgtcgatcct gtgtttgggg
    16321 caagttggat taatgcagga tcatttgaaa cagcatataa cgtatctcaa ttagcggttt
    16381 cggtacgtta ataaaagaga tcgtttatat actaagtagt taagtatagt ctttatttgt
    16441 ccttgcttaa ctacttagaa aacttgttgc tcatggttgg tagaactaaa aggggtgatg
    16501 ctatgagtac tagagcgttg aaacagcttg ctttcaacga aaaaagtgat taataggtgt
    16561 ggtgatttta acttaggaat gggcgcgaag gtcattacca caccgacata tcgtggctca
    16621 ttttcttcgc ctaactggta taaagaagga gtaacgagag atgaagcatg aaaaaacgat
    16681 aattttaaaa tcaaaaataa tttattgttt tgcgacttta gtaatggtgt tatctacgct
    16741 tataatgcct ttttcagcaa taagagctga agcagatgtc acaaaaccga caagtgcaaa
    16801 atttacagaa actggcgtaa aaacagacgg ttatattagg gttaatatgc cgaatcatcc
    16861 aaatgaatgg atgatttcaa gtcaatttaa agattctcat ggaaatattg gatattgtat
    16921 ggattcagaa ttgccatccc caacaggaag tggagccgga agcttgaagt acaagggagc
    16981 tggcagtgat gagttctatc gtatgtttaa aggtggtttt ccatcaaaaa cagcaaagga
    17041 actgggtgca ggaaatgata cagaagcatg gtatgcaaca caattagttt catgggtttt
    17101 agccggaaac tttaaagttt cacagattgt atggtcacat ccaaaccata ccgccgcaga
    17161 aacagctcgt gtaaaaaaag catttgaaaa aatttatgac tatgctaaga atggaaaaga
    17221 tacgcccaac actgagtttt ctatcacagc ttctaagaca gctgacgaag gaaaatatca
    17281 cactttcaca tataaaacag catcaaataa gactggtaat gcgaagctca cttttacatc
    17341 agcaaaacca gctggtatga agatttatga tgcagatggt aaagaaatta ctaacaacac
    17401 tgtgaaatta aattcaagtt ttactattaa agtacctgtg actacaccaa gtggtacttt
    17461 gtcatttaaa ggtactgcta atgttagcac aaccaatcct tttacttttg atggacgagg
    17521 tgtgtatcaa gatgccgtag ttatgatcac tacttcagaa acaaaggact ctaaatcttt
    17581 gagtgctaaa tggacaagag ctgttggtca ggtaacagtt aagaaagttg atcaagatgg
    17641 aaatgcttta aaaggagcgg agtttactct aactgacaat aacaatgcta aatctgttaa
    17701 gacaaccgga gatgatgggt tagctacgtt tgatttgcaa caaggcttaa catacagctt
    17761 agaagaaaca aaagttcctg aaggttatca aggttcattt aaaaaggatg gtattactgt
    17821 taataccgat ggacaagaat ttagttacac tgctacgaac acaattaaaa aaggtaaagt
    17881 aaaagttaaa aaggttgacc aagatggaaa accattagct aatgctgaat ttaccttgac
    17941 ggatgattca tcccaaacac aagcagaaaa aacagatgaa aacggacttg caagcttcga
    18001 tattgtagca aacaagacct acagccttga agagaccgga gtaccaactg gttatacagg
    18061 cgtattcaag caagaaggaa ttaccgttaa agatgatgga caaacttttg aatatacagc
    18121 tacaaacact ttgaaaaaag gtttagtaaa agttcataaa gtcgatcaag atggaaaacc
    18181 tttggcaaat gcagagttca ctttaaccga tgaaaaaggt gcaacacaag tagaaaaaac
    18241 agatgaaaat ggacttgcaa gcttcaatat tgtagcaaac aagacgtata gcctagaaga
    18301 aactggagta ccagtaggtt acacaggtgc atttaagcaa gagggtatca ccgttaaaga
    18361 cgatgggcaa acttttgaat atacagctac aaataatttg aaaaaaggtg tagtaaaagt
    18421 tcataaagtc gatcaagatg gaaaaccttt agcagatgca gaattcacct taacggatga
    18481 aaaaggtgta aaagaagtta aaaagacgga tgcaactggt ctggctacgt tcaacattat
    18541 tgcgaacaaa acttacaact tagaagaaac aaaagtacca acaggttaca caggcgcatt
    18601 caaacaagaa ggaattaccg tcaaagatga tggacaagtc tttgaatata ctgctaaaaa
    18661 cactttgaaa aaaggtgtga tcaaagttca taaagtggat gatcaaggga aagcattagc
    18721 aaacgcagaa ttcactttga cagacgacaa aggtgtaaaa gttgtacaaa aaacggacag
    18781 caatggcttg gcaacattta atattgttgc caacaaaacg tacaaaatcg aagaaacaca
    18841 aaaccctaat ggttatacag ggaaatggtc taaagaaggc atcaccgttg ctgacgatga
    18901 acaaaccttt gaatatacag taattaatca ccttgtacct gaaacagtaa gaacaggcgg
    18961 aatcggtaca tcagccttcc ttcttgtatt tctaattgca ggattgagta tggtgctact
    19021 gtattatcga gaacagcaca aagcaaaata aaaaagtgtt aatggaaaag aagtaaatat
    19081 gaaaaaagaa ggctgacgta tgaaatcaaa aaaaattttt tcaaatttaa tgctatttgg
    19141 tactttgcta ggagtgggat tgggcgcaag ctcaatctct tctagccttg gactaggggg
    19201 gcaagtagca tatgcacaac aaaactataa ttatcaagga actggaacga ttcatatcca
    19261 taaagtaaca aatactaatg gggatgggaa ccctgtctca gatattgata acaaagggaa
    19321 agaaatagct ttaccaaatg gtgtaaaacc tcttgaaggc gttgagttct cttatgtgaa
    19381 acttactaaa gatgaagcta aatccttggg atggacggat agtgatacta caaaaggtca
    19441 acatattgat gatgctaaag cacgattatt actaaacaaa aaatacagtg aagtaagtag
    19501 aatgaagact gattcacaag ggaatataga catacctata caagtaaata cagacgattt
    19561 aacagataac ttgtatttga ttgttgaaac aggaacagtc aaaggtgtag ttgaagaagc
    19621 gtatcctatg attgttaacg tacctgaact aaacggatca gatgatcaat actttaatta
    19681 tgatgtacat gtctatccta aaaactattt ccaattaaac tcagcagagc ttgataaaaa
    19741 agacgaagat ggaaaggctc ttgccggaac tgtttttgaa ttgcgaaaag atgatggatc
    19801 tgatcaggtt gttggaactt ataaaacaga cagcacaggt aaattaactg tagatggttt
    19861 ggaattaggt gactattatt tcctagaaac cgacttggga acagatgcta ataaaacaac
    19921 ctatttacta gatggtaaaa aattcaaatt tagcgttaaa cgtggggaga ctgctaaagt
    19981 agttcttgat gttaccaact acaaacgacc tgtaattact aaggtaattg ataaagaaac
    20041 cgttgatact ggcgaagatg ttcaatatac attgaaacca tcaattcctg gtaatattgg
    20101 taattacgaa aaatatgttg tcaaagatgt tctaggtgca gagttaactt atgattcatc
    20161 agaagtgatc gaaggaatga ctttagatac tgattacagt atcgaacgtg acggacaaac
    20221 gacaaactat gtctttactg aatcaggtat ggataagctt cagaaacaat ttgatgcagg
    20281 ggatcgcttc tttacaattt tgatgaacgc tcatactaat agtaaagcta aaatggatac
    20341 accacttaag aatgatgttt caacgatcta caacaatgga tatgaacccg aagacgaaac
    20401 accagttgat cacgcttata caatcattgg tgatcaccaa ttcattaaaa cagatggaga
    20461 tacaagcaaa gcattgagtg gagcaaaatt tgtagtgaag aacaaaattg atccgaataa
    20521 agttacttac ctaaaacaag atccaacaac gaaagctgtt acttggattg cagataaatc
    20581 taaagctact gaattcgttt ccggatcaga cggtaagttc caaattgaag gcttgcaata
    20641 tacctatgat gtagattttg ataaacaagg taatccaaca atcacagata aaaaaatcaa
    20701 tgattatgct cttgaagaag ttgttgctcc ttcaggatat acaccaacta gaaatgatgt
    20761 tccatttgtg gtatcgaatg atgatgcttc tagcgaagta acagacatca agaaccttaa
    20821 aacacctgaa acagttcgta caggtggaat tggaacagta gtatttctag tggttgcagg
    20881 tgtggcagga acagggctat atgttgtcta caaacgagaa caacacaaag cttaatgaaa
    20941 taaaattccc tagagtgttt ttggctctag ggtttctttt taaaaagtgt gtgaaaagta
    21001 ggtatgagca tgaaaaagaa gaatatcaag ttgatcatct taaaaattgg gatgttgcta
    21061 tgtttaggag taggtctata tcctgtaatt actcaggctt atacctacta ccaaactcaa
    21121 caaacactaa actcctataa ggaaaaatat gatgaagctt ctgaaaagga gaatgaagaa
    21181 gtcttcaaga gatattcaga agttaatagg aaacgaaaag acacagaaca aacctcagtc
    21241 acgattcctg aagataataa caatattccg tcaaatgtaa atcttcagaa ggataaatta
    21301 ggtgaattag tgggtgaaat tcttatacca aagattaatg tggatttgcc aatttatgat
    21361 ggtgtaaacg attttcaact atcagcagga gcaggaatat taaccggaac tgattatcca
    21421 actggtggga ttggaaacca ttctgttata tcagctcatt cgggcattcc taataaaaaa
    21481 tattttacag atttagagtt gctcaaactt ggtgataaat tttttattga tatttcaggg
    21541 aaaaagcttg cttatcaagt agatcggata aaaacaatcc taccaaccga atttgaggat
    21601 ttgcttcctg tggcaggaaa agattatgta acacttatga catgtacacc tgttggagat
    21661 aattctcata ggttactcgt tagaggggtt agcatacctt accaaaaaag tattgttgag
    21721 aaagaaaaga agaaagaaga tttaaaggta aaggcgcaga tgaactggta tcctatcgta
    21781 gttgtagggg ttttattaat aatagctgtg atagtcatta ttaaaaaaat agtagcaaag
    21841 agacgaggaa tgaaaaatga agaataaaat actacttttt ttgactctta tttgcacttt
    21901 ggttttaaca atttatcctg caaatcagct ctatcatcaa ttcaaaacaa aagagaatgt
    21961 ggcggaaatc caagagaaaa ttgagaaaaa acaaactcct tcaaaagagg aagtggcaaa
    22021 aaaagtcgaa caaataaaat cgaacagcgg taaccaagga ttagacaata ctcagaaaac
    22081 agacggcgga tcatttgata ttggtgaagc tgaaggaaag gtcaatcctt acacaggaga
    22141 tgttttttca aaaaatcaaa cgaaagattt tgaaccatct acctattcag gggcggttgg
    22201 ttatgtctat attccaaaaa taaatgtgct agagcttttg tttctaggcg cttcacagga
    22261 acatttatat agaggtgtgg cacagatgag tgctactagt ctacctttat caggtaaagg
    22321 tcatcaaaca gtaatagcag gtcatcgtgg cggttatacg tcaccacaat ttctttatgt
    22381 tgatcaatta gtagccggag accacattta tatagattat cttggtgaaa aagcagttta
    22441 tacggttttc cgaacagacg ttattagaaa ttacggtcag aattcaacac tgactgatat
    22501 tcctgattta gatatgttaa cactttatac ttgtcatcca tatcctcaga actacgatcg
    22561 tttattagtt agaagtctaa gagatgaaaa agcaaaatac atcacgccac aagtaacagt
    22621 tggcaatacc gcatacgctg atgccattga tagcgagtcc gtttcagata agacagaaac
    22681 tgttacatct tctaatgatt tatttgagga tgttgatcgt gccaacaagg aattagatgg
    22741 aaataatgat cttaatattc cggaacaagg tagcgtagag atgcaagaca gaacatttgc
    22801 tgaaaaggca agttcaatat tcacaggaat taccaataaa catcagaaaa tgcttttagt
    22861 catttggact gtaataggag cattagcaag tctaaaggtt cttattcttt taatcaaaga
    22921 gtttcgaaaa gcataatgaa gattataaaa attgatagag tacagtaaaa agggattgat
    22981 actattattg atctcttttt tattttgaat agagctagat tcacttttaa ataaaatcaa
    23041 aactgatttt aattctaaag aaaaagtcaa gaaaaaaata caacaaattt ttttatcaga
    23101 atatttatca gaatattatt agaaaagata aactcaaaag cttaatttaa tagggttatt
    23161 ggttgtattt agaaataatg tatggaaaag ctataaagaa atagataaga tgtagaaaat
    23221 cttatttgtg aatgaaaaac aagtcatcgt aaaatattaa taggaggtgt agtaatggtg
    23281 ggaatatcaa aaacagcttt agactcatta gctaaactat ttagtagcag gtttgatagt
    23341 gcagaatttg ttgaatatct aagccaaaac gaaagtggaa aaactctgat cgaaacaatg
    23401 aaaaaagact atttgaatga aaatgacttt ccgacatgga cgaatattga agatgctaca
    23461 tgggatgtag tgtgtacgat atattctcct tgggtaacga ttgctgattt tgttttagat
    23521 aaaattggag gggatacata ccctgatgcc aaatctcttg ctacaagtga ctttaaaacg
    23581 ttctatggtt atctaatagg tctaataggc aatggaatag atttgaaagt tacgccaaaa
    23641 caaacacctg aagaaattga aaaagaaaaa ggagaggttc aaatggctta tgcaaatgac
    23701 agagaaaaag taaaattatt ggaaggtaaa ttatctgaag aagaacttgg aaaaatggtg
    23761 caagaaatca atgaagacag caactatttt cctgaattga aggtatatca aaatgacgaa
    23821 gatttcttta atgaaatgtt tagtaatagt ccatgggaag cagtgagtaa aacaaattta
    23881 ggagaatgga actttcaaga tgatctagtt gcatttagca atgatgggta tttacgatca
    23941 tattcagaaa gtgagtatag agctgaatta aaaacgtatg aaacggatat tctgatgagc
    24001 tatttaaatc tattagagag tggtcaggga gagttaagag ataaagttga agaactatta
    24061 aaagaagagc tatagggatt atttcaacat aaacacaagc agaaaactac caaacaaaac
    24121 gtaccatgca aaaagtcggt taaaccggct ttttgctcta ttatgagagg agatcaaacc
    24181 atgagtgcaa caaccgatat ggctttagaa gcagtaataa aagataatac aggaaatgtc
    24241 gaaaatgtag aagactttag cgatattgca atcaatcttt cagcatggat ttcagctgta
    24301 ggagaagaaa atatcgaatt tgagatcaag aatatggatg acttaaaagc aaaatttcct
    24361 tttatcgctt cgaaactaag tcaatttgcc gaggataaat cgtggaatga attagttagt
    24421 ttttccgaaa aaatttatga tattacgcaa gaagatgagt gtgtttcatg ggaaatgaat
    24481 aatcaataca agaaaattga tgctttcgtc aaatcacgta tagaaggaac agcttttgag
    24541 tttgaaggta tagataccgc agataattgg ttaaataaag aatcctttat taaaagtata
    24601 gcggatgatc ttgaaaaaca atggtctaag gaagactatg cagagtacct aaaaacagaa
    24661 agagatacag aggtgaaaga aaaactcact gaggtatgtt tggatgaaca tattgatccc
    24721 aatatcgtaa aacaagaaga ctatgaagtg gttattgata aagttcctac tgattttgag
    24781 gaagttgtaa agtatatagt tgaagaagca ttatcgcaat actcttcaga tgaggaagtg
    24841 gacgaagaaa ctgttcgtac cagagtttgg caactggttt ttgatgacaa agattataaa
    24901 tatttgagtg atcaaaactc ggttaattgg agtgtacgat tattaattga tcaaaatgat
    24961 attgaataaa atagagctga aaagctctat tttttttgat aaaaaaacta gctatttttt
    25021 taaaaataat ttggttcaaa atttattttt ttaacttgat aattttttgc catccttttt
    25081 taaattggct atatatcaag gtttatagtg gggataaata aaaatacatt tgacaaaatt
    25141 tacaatatag tttattatat atttatctta ttatgataac acaataaaag gaatgatggt
    25201 atgaaaaaga agaagtttag tcgatcgttg ttaggaattt tagttttagt tgcggttggt
    25261 cttagtggca agggttactt tcagaataac agcactcagg aacatgattt tcagatcaaa
    25321 agtagtgttc aggtaggtaa taaagcagac agtgaatcaa aagaattttt agggatacgt
    25381 tatgatccaa acaaatatcc aaataatatc gtaaacatta atgatgggaa atatacactg
    25441 acaaaagaag accatgaagc attatcagaa aaaggcagtc aaaccttttg ggtaaattat
    25501 gcagatttag atcaacttgg aagagcagga gatgttgaag cgttgatcac ctatgatgct
    25561 ataaattctc actcatctaa aaatgtggaa cgtccatctt tcaaaagttc tgtccatatt
    25621 gcaggagaat acaaggatgg agtatttgat caagagagag atacttgggt cggcaaagat
    25681 tcaaacaatg aaatcactca gctagaagga tataaaggtt atctctataa caaaagtcat
    25741 tcattagcat ggtcgctagg aggagatatg gaggtacaca atttaacact tggaacgaga
    25801 gcgcaaaatg ttgggaaaaa tgatcagaat ggagggatgg cttactcaga aacgattgta
    25861 cgtgatgcaa tctataaaaa taagaatgaa aaagtattgt ataaagttga tccaatttat
    25921 aaagggaatg aattggttcc tcgtggttct catgtcgaag ctttctcagt taacgataat
    25981 ggaaaaacgg taaaccttaa tgtttatgta tttaacaatc aaaaaggaat caacatcaac
    26041 tatcaaaatg gtacttgggt aagagattag aaattgagaa ggtgaagaat tggaaacaat
    26101 aagcatcaat ggattagata aatcagttgc aaatcgaata aaaaaaggaa atggctacta
    26161 tcttctgaag aagggtaaat attcagttgc tagagagtta gaaacaatga gtatcgtgta
    26221 tgtagctaag aatcattttg acgtagaaca ggctattttt gatccagatt ctgaaatagg
    26281 gaaatgtgtt gaattatcag agaaaactgt tagtacaaaa ggggcgatga tcattgagag
    26341 agaaatgtta gacgaaatca gagtaatcat tcctaaagat acgcagatgt gtgaggacag
    26401 cataagtgac gatgaaccaa aatcagaaag aaaataagga gatattcaga tgagcgaaaa
    26461 atccattgaa ttaaaggaat ataagcgaag cgatggagaa aaatattatc ctgtagccag
    26521 tttttctaaa tcagtatatc tggttggacg tgcattggta gttggtgcaa ttggcatata
    26581 tattttgcat tgggtaacaa aggttgtaca actgcttgta gataccttag taagtattct
    26641 tgtctcgtta aatgatgtta acggaacaga tgatttagga ttaaaagaaa tgtttaaaga
    26701 gttactcaat gcgttttttc ccaattattt aagttattca tataatgtac aaagcttttt
    26761 tgcaaagcta cacttggtag acatctctat tttagtatta gaagcaggaa cggtattctt
    26821 tgttggatat ttggttactt tgatgttcag gcatcactat ggagaacaaa gaccattttt
    26881 tgatgatatg gaaagtagaa ggttgaaaag agaagctcta gaagctatgg atgtgaagta
    26941 tgatccaaac aagattatag gagatgaaac gaacaacaaa aaaagaaaac gaaacaaaac
    27001 gcaattagaa tccgatgtgc tatcaacgct taaaaatatc aaaatttctg ttcatacaag
    27061 acgtgatcta agtgtcaatg aagatattcg agaatatatc gtaaaaatcc cgcaaccaaa
    27121 agggaagaaa gttagagaaa cagttttacg acaaattcaa gatttagggt cgatcctgac
    27181 aagagcaaca agcggacgag ccaaatttgg tgaaaaagaa gatgaagttt caggcgatta
    27241 tttcattttc cgagcatcag taaatattga aagagaacgt aaggaaagag taagtaccaa
    27301 aaacaaagaa acggaaagag caacatcttc aatcgcagtt tcaaattcac aattttcttt
    27361 tccaatcaat ctctttgccg ataattccaa aaatatccaa aaccaaaccg aaaaagctag
    27421 aaaatatgcc gaaactcaaa agcaatcttt agaactttat ctatcttctg ctgacgtacc
    27481 tgcaagtcta acagatatgc acgttgggaa tacatctatt gaatatttgt ttactttatc
    27541 tcgtaccaga aaaattcaac taagctcaat gaaagaagaa attgaaactt acttgcatat
    27601 atcaggtgta atcgtgcgga tcaacgcagg gcgtattcaa attactctac cattaggaga
    27661 aaatgatcgt attccaattg atgtaccatc aatgatcaat agcgtgtttt aaaagacatc
    27721 acaaatagaa acagaaaata caaactacca gttcacaaac agaaaagtac cgagaaaagt
    27781 gaggaataga aatggctatg aaattattag agaaagtcaa aaagagagcc gaaaaagctg
    27841 aatctatttt gctagataaa gaacctaata taaagcttaa tggaaaattc agtgtcaata
    27901 tgttgaacac aaaggctaaa gacccgacag atgctatttt tggaattaag cctgatggca
    27961 caattgctca tttttgcatc agcgaaatgc cacacatgtt ggttgcagga acgacaggat
    28021 cagggaaatc agtattactg aatgaaattc ttgttacagc aatgtgtcac tcaacgcctg
    28081 atgaattaaa aataggaatt gtagacccaa aaagggttga atttggaaga tacaaaaaat
    28141 tgccgttcat gttggctgat ccaattacag atatggatga agcttatgat ttttttgaat
    28201 atcttgtgat cttgatgcat gaacgttaca aactgatgga aaaagcaggg ttacaaaaca
    28261 tcacgctata taacgaatat gccgaaaaaa atggattaga tagatttccc tatgtgatct
    28321 tattagttga tgaatattca cagttggttg gaacacataa agaagtagaa ggtttgattg
    28381 ttcaattagg acaaatggca agggcagcag gtattcatgt tatcttagca acacaatctc
    28441 ctcgatcaac agttgtcact ggtattatca aagccaattt tccgtcacgt gtttgcctta
    28501 tggtagcaag tgatttagaa agtaggattg tattagatga aggcggtgga gaaagtctaa
    28561 gtccaaaggg agatatgctt attaagcttg ttgatggaag tatggttaga gcgcagggag
    28621 cttacatcag taacaaagaa attgaaacaa tcttcaatca ccttagaaat actatgccag
    28681 aaccagaata tgttgattac cgagcaatcg tagctgaaac tcgtggtgag ggcggtgaag
    28741 acgaggattt tggtggaatt ggtaaagctc ctgtaagaaa atctgctaaa agaaatttga
    28801 acacaccttt agcaaatcca tcatctaaag agccatcaat tgaaaagaag agtgcgtttg
    28861 ctattaagct tgaaaaaaat aaagaagaaa aagctaatca gggagctaag tcactagaaa
    28921 aacttcagca agaaaaatta gaaaaactag aaaaagcatt atcatcatca acttctgaaa
    28981 ctcaatcttc aaagggtata gataatggac aatcaattac aactaaaaac aagcctaaaa
    29041 atagtaattc aaatctctcg gtaaatgttg ttatcacaac tgataaatct gaaaaaaaac
    29101 aagtgaacgg aactacttca cccatcatgc aaaaaatgat aaaggcaaaa aaacaggtgc
    29161 agaaatgatt atcattagtt cctttttgag atggctaaca ccatataaat atacagatga
    29221 aatagttgag aagcgagcga agaaaaaagt taaaaaaacg atgttaactc ctaaagaaat
    29281 gctagatcgt ggaatattgg gagtgaaggt tttagaagta aaccaaccgt ttgaattgga
    29341 taatgcgaga gataggttgc ttagagctga agttgatgtc gcatactttt atcatgataa
    29401 aagcaagcat caaatagcta tcaaattcaa tccaaaatcg gcatggcgat accaagcaag
    29461 agatgaccta aagcgtagga agctgaaacc gatgctctat cctgaaggaa aagaggcatt
    29521 tgatagaacc catattgttc ccattggatt tcatggtaca gagtcagata accgcctatt
    29581 ggtagggtgg aacagctcac aaaatagagt tcagatcaat aaatttgaga agaaggtaag
    29641 aaaatttaac gatcggaaaa taatactttg gtttgccaat attcagaagc aaccagatgg
    29701 gacagcaaag tgggaagcgg tcatatttga tgaagatggc aaaaaagtaa tgtctgaagt
    29761 atggcatgat aaagctaaat ttgtttggca gtagaggtga aaattgtgtt aactaaccta
    29821 aaactatata gagagggatt taacgaaaaa gtaagtatcg gtgaatctat tgaatattta
    29881 ggaaagaagt acattattat tcgtattctt gaaattaaaa aactttattt tagtaaatat
    29941 aataatttag cagttagagt tctcgtccaa aaggttggaa atccaagtga ctatagaagt
    30001 gattaccata actataaaac agaatcatct gtaattaagc gttataatca atcaaaaaat
    30061 gatgagagaa tattcaaagt aggagaaatt attatttcta agtatgggat agcatacgaa
    30121 atagtttcta ttaaaaaatt ttattatgaa tttgttgatt tggttgtaga atattctgtt
    30181 cgattgatcg ttccgtggag taaagaagaa attaatgagg cattaaaaca tgaacgaaaa
    30241 agtacattca aagttttaga aggtgaaaaa aagtgaaaac agaccttact aggcaagctg
    30301 agaagtgctt atggcatgaa acaaatcgca tgggtgtatt tggttgtttc gaagtcacta
    30361 tcggttggga tggcagtgaa agagtagatt atataactta ttcagtagat aacacagttc
    30421 gatgttatga gatcaaagtt actttagctg atttaaaaag caaggcgaaa cagactttta
    30481 tcggtgatta taactattta gttgttacta gtgatttgtg ggagaaaatg caacaagacg
    30541 ataaacttat gcggaaatat tactatcaag gcgtatacgt gttcaatgaa aaatatccta
    30601 gcataataag tgttaagaaa gcaaggaaac aaaaagtatc gctaggtaca agatcaactg
    30661 tattagaaag catggttcgt tctatgaatc gtgaagttaa gaaactctat aaagtaaaac
    30721 cattttggga gatagcagag gtggaaaaag gatgattcaa acatttgcaa aaatgagtag
    30781 aagtgcttgg attgaaaagc tggaaaatga tttgagtgtt atgcgattag ctccaaagaa
    30841 attcaaacga gagattgcag aaaaagaagc aattttgtcg gtattgaatg cggtagaact
    30901 ggacgaaccg aaaaagtcag ttgatgtacc tgaaaatgta gcagagttac tagattatta
    30961 tcgtaattcg tcagatgtcg atttgttagc tctgatatta acatttcacg attggtatta
    31021 ctgtcaaaat gaagatactc agcatgataa acatgataaa gcagttgact ggttaataga
    31081 gaatccagaa aagttcatgc gtgcttggct agatggctat gatagcaaga aaaagccact
    31141 ggaaagtaat gaggaaacgt atttaacggc agaacaatat gactatgcta gaaaaacagg
    31201 agattgggaa tctggtaaga ctttcaatca cagaaaagat caaccaaaaa ttagtttttc
    31261 agatatagcc gcaattatcg aaggtatcta ctatggacaa gccgatgaag agatcttttc
    31321 tatacaagat ttattagaat ttttgaacaa aaataaggat agctttgtag aactaactga
    31381 ttatgaggag gatacagcaa atggaaaatt atgatccaaa tatgcgatgg ggtacacata
    31441 cgcttaaagt ttcttttcaa cgttgggatt acaagggatt tgtgactttt agaaaagctg
    31501 gaaattgtaa aggattagat gtcttagcgt tggatgaaga ctatctatat gaccatccat
    31561 tgacggataa tccaatcgga tttggattgc ttcctgaaga tgatgagggc aatgaatggt
    31621 tcaaaatgat tcttacaaac gataaaggtg atcaattatt tgtagaagat acttggtcat
    31681 atttgagtga atatattgtt agcattaaaa tcattgattt tgtagcagat aaagaaaaag
    31741 agattggaga aggtaaatct aattactagc taatgaatag caactttgga ggataagtca
    31801 aaagaataaa aaaagaatac tgtgtgtcat tgcagggatg tatctttgca tgatagtatc
    31861 tgcggttacc atttattggt taggaaatca gcaaaacagt ttaattgttg atctggggga
    31921 gttaaaggtt ggattacgaa tgactagatg gcttttatta gctcaaacag ttggtttagc
    31981 catattgact tggatatgtg tggaaaaaat aacatctaat aataatcaag agattgttga
    32041 atcaacaaac ctaagaataa ctgaacaagc aattgaaaag caaaaagaca aagaaaaaaa
    32101 ggaaataatg tcaatttatt tatctagtgg agcatcattt tttcatgaaa tagattcgat
    32161 gaaactgagt agtcaagaaa tcattgatag atggaaaaac atgcctctag gtggtgagtt
    32221 aatcgttgaa gatagtgatg gttggtcagc ttataagaag gacgagattg tagctatcac
    32281 gattttgttt gaatagtaat atattaccac tagataatct gaatatgttg atctgctagt
    32341 gacgtaccaa atgcgtttaa tagaaccttg gtcacaggca gaaattgata aagcagtaaa
    32401 attgaaccga ttatcaaaat ttaacgtatt aggaaatgcc ttttaaaaat cgcaatacac
    32461 aaacagaaaa ataccaaaaa attagcaatg aacgtaggta agaacatgac aataaataat
    32521 aataacccta acaaatataa tgctcttgtc caatgctata tcgaaaacaa aattacaatg
    32581 caagaagtcg ctgatgaatt aggcaaatca aaaagcctag tatcacacga ctttaagcac
    32641 aatgtttttc cgaacccgat tgagctgagg aaaaaatttc aaaagagagt ttatcaagaa
    32701 atagggaagt ggattgatga agggaatgca attgaccctt taattgaaaa atatcctttt
    32761 ttaaaaagca aaaataaaat tcaaaggtta attcgtcaag gcgatattca agctaaagaa
    32821 cctgttttga caaccaatga tttggctaaa ataaatcttc ataaacagat tattgaagac
    32881 tttgaaaaaa atctttcaac aactgaaatt ttaaaaaaat acaatattcc gagaggaaag
    32941 ctaacatcga ctctttttag agaatatgga gaatcggcag ttactaaagc aaaggaagta
    33001 gcgaatgaga gaaaaaaaca aaaatagtag tcgatactta gtagcgactg tagtttttgt
    33061 ttgtgtattg attttcctgt ttgatttttt acagattgga aaaagtataa ttggaaatac
    33121 attgagtgca aaggaaatgg ttgctttagg tggtttgtat gaaggacatt tgttcccgaa
    33181 tatattcatt tctatcgttt ctcatattaa cttgtaccat ttaatttcta atttattgtt
    33241 cttagtagtt cttgaagaag cactgaatga tcagtatgag tggttaacat tattgatcgt
    33301 tttaatcata tcaggatttc taggaagctt agttcctttt ctgctacaaa gcaacatcgt
    33361 aagtatgggg cttagtggag gtatttttgg atggattgct ttatttgcga ttgcatacat
    33421 ccattttgat agcaaggaac atttattact aagtgttttt gtattattga taatcggcat
    33481 tttattggta tctacttttg aatcaggcga ggcaaatgtc ttgtcacatg taatcggatt
    33541 tgtctgtgga atgatcttgg gatttattct aaagaacgtt tcattgatca aacaaaagaa
    33601 ttaaaaggga gtgctaaaaa tgccaaaaag aaaagaaaag aaaattccag tgtttttgtc
    33661 tgtctcagaa atgttatcac ttcaaatact gttaagaaaa aagattgaaa caatagacga
    33721 agaagttaga aataaaaggg ctatgtacga acaacgtgaa gcgctaagag aacgaatcca
    33781 ctattttgat gatatacaca ctaaaatcta tgaagcacgt acaaatcacg aagacgaaag
    33841 taaattagga ttagctcaac aacggatact tgaatggctt aatacgaata ctgatcaaga
    33901 accgttaaga gtgattagta atctttttga atttggaacg aagcattggt ttaatgctgt
    33961 agagaaggat gctgacttac aagaagcgtt tgatacgcta agtaaggata gcaaatccat
    34021 gcgagattta ttgattactt atctaaatga gcatgagttt gaacaaacga aaaccaaaaa
    34081 gaaaaataaa gaatagaaag ttgtgatgaa tcatgctatt aaccctgtta ggttatttat
    34141 gtttaattct tgcttttttt ggggcaatcg ctataattat tggtcaattt caaacaaata
    34201 atccgattat gcgtaacata ggcattggaa ttgtgattgt aacctttatt gtgtttaatg
    34261 ccactaagat ttttggaagt aacgataaac aatttggtat atcgggagtt aataatagtt
    34321 atgctccaga ttctttggga agggtagtaa tagatggaac atttgaaaat gtaacgagtt
    34381 ataaaaatgt ttcgctgaca gtgaaggaag atggcaagaa agcatcagac gacgttctcg
    34441 ctactattaa gccgtctcaa tcacaatttg aagtgtttta tacagttaaa gagaatagcc
    34501 ctgtacacaa gctgacattg gtttttaacg ttgatggaga agaatactat aaagatgtaa
    34561 aaataaataa aacatcaacg aaaaagatta tcgaatcagc gaaaaaagaa gtagctacag
    34621 aagaaagtac aactgaatca agttctactg tcgaagcgga actaccagat aaaattgatt
    34681 ttgatcctga aaaagtcaaa tcgtttgaat ctgtttctgg ttctatggct gatcggttta
    34741 acactgaagt cttcaaagct atcaaaactg ttaaattgga tactgaaaag caaatgatta
    34801 atgttacctt attcaattca gttgaatcta tggatcagga taaaaaagtt acggttgcaa
    34861 gagaactagg aaaaagagta attggtttgt atcgtggtac tgctaaacca gattcagacg
    34921 gtgcagattt gccagttcat gtagtttatg aagacggtaa agtttttgga gattctacag
    34981 caaacgaagc attaacgatt aaattggcta aatagccaga aagttataat aaacaaatgg
    35041 tgtcacacca ataatatata ggtgtgacac cttggagatg atagctgatg gtagtaaata
    35101 aagaaaaagt tagaaagtat gtgactttaa aaatagcttc tgttaaatat ttagaaaagc
    35161 gagctaaaga agaagaaaga gcaagaggta agaaagtaac agtttcagag ttgttagaag
    35221 aattgatcga gaatgatcaa acaataagac gtgcatttac taataggtct atcagataac
    35281 aatataatta ataaaaaaga aagagtgatt tggtcttaga ctaaatcact ctttttatat
    35341 aaagtttctt tacactcatt ccgaataatg ttaaaattct ttatatgata tatagttcct
    35401 tgtcagagga agagataacc agcaatattg gagggataat aagtggaaaa caataatgat
    35461 attagacatt ttagcattgg ggaaagagtt acaacagtaa gtgaattatc aaaggattat
    35521 agagataaag agggaacgat tatttctctt aataagaatc gaagaggcgt ttggcaatat
    35581 gttgttgatt taggtgatgg cgttacagtt gagtgtaaag atagtcaact gatcagaaat
    35641 aatggcagtc attccattga aaaagcaatt cttgtatacc caaaagtatc aagtaagatc
    35701 atgaatgact ggttgcttgg aaaatttggt gagtacaata aaaaagaaat cgcagaagtg
    35761 atcggcaaat cttatccaac agtattaggg tatctaaaaa agataacgag taaagaagca
    35821 aaaattgagg atgttttaac agtaagagag cttcaatcat taatcgagta tcggaataaa
    35881 caaataacaa atcctgagta ccttagaaaa gaagatggtc tattgaaaca attggaacaa
    35941 gtaaataaaa tccggaagat gcaaaaggta gctcctttaa gccttgatac ctttaaaaaa
    36001 atctatttga aagagatcaa aaaagactaa tgtggactgt aactttaccg ttccaacctg
    36061 tatctgcacc aagaccaagt gcaagaagga atgctgctga tgaaactaat gacgtagcag
    36121 agaaaaggat ttcaacctac tatgataaaa agtatcttga ttatttgaaa aaaataaaga
    36181 gctttatcga agatcaagat ttattagatg atgaatttta ctcgattgta aatgatccta
    36241 tgggcgcaat tatggaagtg gacttttact accagatacc aaaaagctat aaaaaagtct
    36301 ataacattat gaagacaaca gcgccagatt tagataattt agtaaaaagt gctatggatg
    36361 gtatttttaa catctcagct atacgagata gccatatcac aggcttaatt gcttttaaat
    36421 ataatgtggc gaacgaagga aaaacagttg tgaggatcaa acgatattca gaatttgagt
    36481 gggatacttc tgaaggattg aacggagata tatgggaagc tacatttgat tttaaaccag
    36541 tagcaacgcc aagaccaaag tctaaattta gaggaaatgg gattatcact tactatccaa
    36601 aagagtacaa cgactatttg gaaaatatca agaatacgct taaagagaaa gatttggtaa
    36661 atgatcaact gaaaaaggta atgaatgctc ctatgggggt tatagctcag atagattatt
    36721 tttaccaagt gagaaaagat aagaaaaagc ttgattcact gatgcgtact tcacagccgg
    36781 atattgataa tttagtcaaa ggaacattgg acggaatttt taacaaggaa ataggcatca
    36841 aagatagcag ggttgtaggg ttaattgctt ttaaatacaa tacttttgaa aagtcgaaaa
    36901 caaacgtgag actacaggag atgggctgat gaataataat gaaaaaattc ttcatgtact
    36961 tgatagcttt gaaataattc aagaagaact aaaaaaatat cgggatgttt tggaacaaag
    37021 atatgatttt gtcaatcagc aaaagagtaa tcacatggat ttcattctca atatgaatga
    37081 cttgaagaag aaattagttg aacgaaaaga acaagaaaag ttgatcaaag cttactttga
    37141 actcggtgaa aaagaagtta aaaatgcaat ggaactgaat gaagagcgca gggttttaga
    37201 tcagctgtta gaacaattat tggtgatgtt tcaaaaagga cgaatagatg aggatttaat
    37261 tgaagaagga ttgagaaaat atccttcaaa ctcaggtatc ggaatagtgc tgaaagcgat
    37321 tgatgaagaa gaaattgaag ctttcatacc gccagaggat tttgaatcgg caatggaata
    37381 tataaaatat tatagccaag ggatcactgc ttttagagag tttgatcctg aagatgttat
    37441 tcatgacttg aacaacctaa aagaatggtg tgaaagttat ggagtcgatg attccggatt
    37501 ggactacttg atcagtatta tggaaatcga agaagagatg ccggataagc ctgatccaac
    37561 agatatttta gaattgatcc atgaagctag aaatccaatt gcctatattt ctagaggata
    37621 tactgtattg gaatactata agccttatat atcagcgatg aaccatttaa gacgtgtatt
    37681 aagggaaaaa cgtgaatacc gctcagtgtt gaatgcaacg aatagattag aaaaagctgt
    37741 ttcagagcta gatgcttatt atcgtgaaca ttacctgcaa gcaggtggta tgccaagaaa
    37801 tacgaaagca aatatcagta ggtatataaa aaaagctgag taggcggagt tagcccataa
    37861 aatggcaaaa ggtaacctga aaggggcatt ttgaatgaaa agggatgttt ttgaattgta
    37921 cgtattagaa cgtctagaag acggcatgat ggcacaagag ataattgatg aaattgatga
    37981 tatgatcagc agatttggtc aattggagtt gaatgatgga aacaaaaatt cggtagtgct
    38041 aaaacaattt tggttgaaca atttttcgta taaggatttt atcaaagaaa aggcaaatac
    38101 attgtactga ctaaattcac gaggagcagt gatgagagct tattgctaag agcagttagg
    38161 ctcttttttg tttgtgtaaa attatttgtt ttataataca cgtttataca caaaaaatat
    38221 tgacagatag tacacaaatg tggtaggata tatttgtaag caagttagat taacaagtta
    38281 aaggaagagc agttatgaaa aaagaagaac taatagatat atttcaaatt gttgaacgag
    38341 caaataatat gggaattatg ttttttgatc gtatttcttt aaagatggat ttaagcgttg
    38401 cccaccaaga atttaatctg cgattaaaag ccttgttgaa ttctgatgat gttaatttcg
    38461 ctcatgatgt tgtaggtata cagaaccata tagatagaga aaacaaacgt atgggtgatg
    38521 aattcttacc aagatattca agtttatgat ttggcgaaag attcaagagt atgtgtcatt
    38581 attgataggt actcttgaat tggaaaggag gaatgaattt aagacaacaa cgattatgat
    38641 cttaaaacaa tcgtttatgt aattggcaat cttacgaata attttcatgc aataatgaca
    38701 ataaaaaatg aaaaggggta aaatcaaatg aaaaaatttt tagtaggaat tctattcaca
    38761 atggggctag tattattatc accttcagtt tcagcagaaa gctctatttc catgtatcgt
    38821 atgtataatc ccaatagcgg agagcatcta tacactagaa gcgttggaga aagagacaat
    38881 ctgaaacgtt atggatggca ttacgaaggt gtcgcatggc aagctccaac aagtggtact
    38941 cctgtttatc gtttgtataa tcggtataac ggtgaacatt tttacacagt tgatgccgga
    39001 gaaagagata agatttcaca cacaggatgg acgtacgaag gtgttgcgtt ctattcctac
    39061 aacggagcaa atagagttcc tgtaacacgt ttatataaca aatacgtgaa ttggcatcac
    39121 tacacgttag atcaaaatga aaagaacgta attagtaaac aaggttggaa gattgaaggt
    39181 acaggctggt atgcaatgcc aaacagcgta ccaaatccta cacctgctcc aagcactgaa
    39241 aaagtgttga acgttcctgt tgtatggcaa ggaaatacaa tgttatgcga aggagcttct
    39301 ttattagaag gtttacacta caaaggtgta gccacaaatc aaagcttaac cagttttgta
    39361 aactatatgc caaaagctaa tgacaataat ccttatcatg gttactcagg agagtggcgt
    39421 cataacgtta atggaacgta tcaaggcatg atggctgacc cagtagttaa atgggcaaaa
    39481 aacgttggcg gtaatgccgt caatatcact ggttcaggaa cgaatggaat caagaatgaa
    39541 attcgcaatg gtaatccggt tgtaacttgg attacttata actatgctac gccagagttc
    39601 aaacaaatgc cttggggtcg tgcagtatgg aatggacatg tagttctcgt ggatggcttc
    39661 agaaatggtg cataccatgt tgttgaccct gtatttggcg caaaatggat caatgcaagc
    39721 acgtatgaga gagcatttaa tgtgactgga atggcggttg ctgtacgtta attgatagaa
    39781 ttatcaacta aatgaaataa gagcttaatt actgaaatgg taaataggct cttatttttt
    39841 tagaggaaat ttttgtaata cgaatctata acaaaaaata ttgaatgtag tacacaaaag
    39901 tggtatatta aaatcgtaga gattatgcga agaggtgatt ttgatgaacc aaagaagaag
    39961 aaaaaggtta gaagaaataa gcgagcgact ttccgagcta aaggatgagt tagaagtaat
    40021 tcaagaagaa gagtacaaag aaaatatacc taaaaatcta cagggatcag aaagatatga
    40081 aaaagcagaa gaatccgttg atcaacttga agaagctgtt gatggtttat ccgacatatt
    40141 agaattgatt gaagatatag ctagctagag atgattaatg aagatttcag gagtgagaat
    40201 aaaatgattg atatttcact gatagagaag ttacttttct caaataagta caccgcaaaa
    40261 gagctggcta caagatggca aatatccgaa tcagctatta aagcctatcg tcaaaataag
    40321 ggaagcgtag gatggcgtga ttggaaaggg atcagcattg gaaaagcaga agacataatg
    40381 aaacgtgaag gacttcgatc ctataccact tctgaaaaag acaaataact taatgtttga
    40441 aaagataaaa tatctgtctt aaagctatgt caaattcaaa tggtgaatgt ttgctaaata
    40501 taatgcaaaa aaaggaaggg aaatattaaa tgataagcga aaatttgtct tatgagaata
    40561 ataaacatct agttaaagaa aataagaaag tagaagatta taagaacatg tatatagaaa
    40621 tgaaaaaatc accactgtgt attcaatata aaaatgataa ttggaagcaa gtagtcgaat
    40681 ttgtaaaacc tcatagtata cgaagagtac gaagggggaa gaaaaaaagt gaacttgtaa
    40741 atagcggaag agcggtaatt agagttataa ccaaagatta tccagttgaa tatggagatt
    40801 atattttaag gttaagttat catgcttttg ttgtgttgaa agaagagcat ttcaacgagt
    40861 ttttcaattt aaaaagtaag taatcatatt tttttgtaga cgtgtataaa gttaactaaa
    40921 ttgcgaggtg atgacatggt agcaaaaaag aatactctaa aagaagcttc tgaaaaatat
    40981 caagatatga aaagacgagg attaataccg aaagacaaaa aaggtatatg gagagaattt
    41041 gttaaggaag tcagtagtga tcgaagtggc ggtgactttg ttggacgata tacagcaaaa
    41101 ggctatagca aacgattaag gatcactgtt gaatatggaa cgaatgaatc agctacagag
    41161 ctgaagcaaa ggtacttaga tacacgaagg gaagcttcaa aaaaatataa tgatttcaag
    41221 agcagaggtc tgattcctaa aaagaaaaag ggaatttggc gtgagttttt caaggaagaa
    41281 gatagcaatc ggaataaggg agacttcatt ggacgataca cagcaaaagg ctacactaag
    41341 aatttaggga tcaaggttga atacggaact ggtgaaagtt catgggaatt gagaaatcgc
    41401 tatttaaata cattgttaga gaaatataaa gcagaattat tggagttgtt cgatgttaat
    41461 gattttatcg agatggctga aaagtatggt catgaagaag gttttatgat caatgacatt
    41521 atggttattg ataattttac ggatttatta gcacttgcga aaaaagcaag ggatttaaga
    41581 gaaacgcaga tgtatctatt cagaaataaa aacggaagaa accggagaac tgaatcgagt
    41641 gagattgaag attactacga tgcctttaca cacgatctgg gagcctttgt agacgagata
    41701 agtggtgtaa tgaacaaata tagggtctag aataaaaaag ccggagatga gcagataaat
    41761 gggaaacaat agcatataca cacctgaaat gcagaataaa attaaaagac ttgtcggatt
    41821 agggtggatg acaagaggaa aattagattc ttttagtacg atcaaatatc ttttgatgga
    41881 catttaccgt cagaacaagc aaacaggaat acattttttg ggctatgata agccggaaga
    41941 tgcacctttc tttgttccaa gttcagccta tagagccagt acctccggta gcatgtataa
    42001 cttggatttg tttgaggaag gagcaaagat gatcgaatat gatttcaacg aagcctattc
    42061 gaggatcatg agaaattatc ctttgccgtc aaacacttat ttgaaagcaa aatataatac
    42121 agatcaagct attttgagag cgataaatag accaaaagta agaccgtatg aaaatttaag
    42181 aacgtatatg ttctttttgg ttaattttga aggaacagcc aaagaaggaa catatgctaa
    42241 tccaaaatca cagtcgatgt ttgcggatta tggcgaaaat cccaaaacag ctaatgagcc
    42301 tatttggtta tcggagattg agctaacact tatccttgat tattatgata ttaaagagat
    42361 ggaagtccta gatagccaca catttttgac gaaaaaagga ttactcaaag aatattttga
    42421 aagaatcgag ccattaaaaa ctgatcctga tacgttgaag ctttataaaa atcttagaac
    42481 gaaagtattt ggtgctatcg gacaaaaatc attaagagaa ggagaaacaa gcaccttcga
    42541 taaatacagt atgtataatc gagcgtattc ctccatggta gcaggcgtat ttagagatcg
    42601 tatggttaga tatgaacaga aatacgttca tagcgcatat gggctattaa aaattcgtac
    42661 tgatggattg tatttcagat ccgaagtacc agagtttgaa gagctgtata aacttggagt
    42721 agttaagaaa aaagttcata caatatccaa agaagaagtc gaaaaagctt tagaatttaa
    42781 aaacaaagga tttgaaaata agggctcctt tgggaaatct aaagatgaag ttaagggtgt
    42841 tgtgatagac gatgatttcg agtttgattt tgactaaata ctctttggaa aagatggtag
    42901 gaggtaaata tgaagaagaa aattgtggaa ggaaatgtag gtgttattat tctaaaagat
    42961 tcaactgact atgctaaatc aatggcacta aaagacgaaa aagttgcacg catggataaa
    43021 gatttagttg aatttctgat ggatgataca gaaagattaa aaggggatca gtttttgagt
    43081 ttaatgaaag acaaaggctt aaacacatat ggtttatttg attataagaa attagctata
    43141 gagtgggtaa aagaaggaga aatatttttt attgaagaat gggaaggtac tgagaactta
    43201 gttcttcatc agtatctttt gaaagcttaa tctaagtaaa acagttctga ctagttatgc
    43261 ttgtcagaat ttcaaaggaa gttttccata accatcatca ttcaaaaata aaggatttga
    43321 aaataagggt tcctttggga aatctaaaga tgaagttaag ggtgttgtga tagacgatga
    43381 tttcaagttt gattttgact agattttttt gaaaggttta agagtaaaca aggtaagaac
    43441 taagatcatg aagattgaga gtaaagaaga tagaggacga atgtatgaga acacactata
    43501 tttatttaga agataaaatt gtagatgaaa ttccggcaaa aagtcgagaa caagctagaa
    43561 aattatttat tgaaaaatac aaagagatca atggagatag tgaagttcca aaagtggtcg
    43621 taacagttag aaaaaggcaa acaaaaaaga gaagtacgtt tcatgtcagc cctgatatat
    43681 ataatcttat agagcgacat agaagtgaag ataatatttc tatctctcaa tatattgcta
    43741 cagttattga tgaattgatt tcaggaaagt atgactatgc ttttgatttt gagggtgaaa
    43801 aggattacaa aagtgttgct gtctttcaat ctgattttga gaagctaaaa aactatgctg
    43861 gcaggagaaa tgttaatttt gcaagactac tagaagagtt ctactccgaa gagttgggag
    43921 taggaaatga atacaagaga gaaacttatg cgaaattgtc tcataagtta tcccaagcgc
    43981 ttaaagaagt gacagaagtt aaagcggaac tagatgctaa acttgaagaa gtgaaaaaat
    44041 atcaaacaga gaggaagaac aacatgaaga atcaagtgga atttgacgaa cagaaagaat
    44101 ttgaaaaagc aagaaaacgc tataacaaaa ttatgaatga acttagctat gaacacttaa
    44161 cagttcatga ataattgagt gagggtacag aaaaatatac gatcgcagat atggttaatg
    44221 aagtggaaac tcttttagct tctttctttg aaaatggtca cgtaaacgat gaatggcgaa
    44281 aagaagaacc taaaagggtt aacgcttata ttgctcgatt aaaaaggttt attgataaat
    44341 ataaggagaa tagtgacaaa ttttctaaag ataaataagg agagagtgtg attacatttg
    44401 ccagaaatcg aaggaatatt ttcgccaaaa ataggtaaaa aacagttaga tttcagcagt
    44461 tttccgagcg taaatagaga ttataatcta ggtgatgagg tacagatact aaatattgat
    44521 ttcaaagaac tagacttcgc tgttgctgtt tccaacaaac ccaaggcatt ggaacgagtg
    44581 aaaaaaatat accaagagaa tgatcaaaat atcaaaaata tgatcaaaaa gtatagacga
    44641 gataattcaa aagttgaaga cgttttattc aacggtacgg caaaacatga tttgaatgtt
    44701 tatatggcat taggagtggc tttgtactgt aattcaatta atcagacaga tattttggtt
    44761 aatatcgtga ggaatagcta tcaaaaaata ttaaaaataa gtagcgaaac aaacccttat
    44821 tatgcaatga aatatcttgg ggataattta ttaaatgata aaaaattgat gcacaacact
    44881 gaagagctgt tatctaattt ggctgcgatt aactttctat tcaaaacagg ttctgaagat
    44941 tattttatag ataacgtact aaaaggtgct tacggaacag cattttatcc gctttacaat
    45001 atgaaacagc ataaagccga acaaaaggat aacaaacttg aaattaaaaa gatgaaagaa
    45061 ctattaggta taggatttgt aaaacccaat tcaaaagtca ctattgcttc gttagcaact
    45121 actaacaatg atgggaaaat agtaaatggg tttgacgatt cagatataga tatattgtca
    45181 aaatcatatt tttctttgta tggagatttt gcagatttga tgggtattga tagttactta
    45241 gtcaataatt ttgagatcaa gggtagcgag ctgaatgata ttgtgtattt cgttttaatc
    45301 tctctatatg caattgatca gaataccgag taccgacaaa ttgactattt gctagttttt
    45361 tatagtttgt tatatcagtt cgttttaact cggttgttta aaaatgctaa ggaagaattg
    45421 atcctgaatt ctgattatga aatagcaaaa caaattgcaa gtattagaaa agaaaacaaa
    45481 tctgaagtaa tcaaattaag aaaagaatta agtattgcga gtaaagaaaa cggtcggtta
    45541 gaatctttag tcaaatcggc aaaaaaagaa gaggaacttt tgaagcaaga gattaaagaa
    45601 ttaaaaggaa tcagaaaaga acttgaacag ctgaggtttg aaaataaagt attaaagcaa
    45661 ttgcaggagc aagaacatag acaatcatta aatggtaaaa ctgaagagcc ttcactgtct
    45721 gaaatgatag cttatctaaa acataaaaac attcttttag tgggaggaca tgtaaatttt
    45781 caaaataaat taactgaact attaccaaat attaaaatgt ttgatgtaag cgaaaataat
    45841 ggaaagatac ttaaaaattt gaatacgtgt gatgcgattt ttctatatac agattacttg
    45901 aatcatggtc tatattatcg tgttatgagt gaaatttcta ataaagataa gatattttat
    45961 ttggataatc atgtgaatat gaacttaaca attaaacgta tttacgatct tttatcactt
    46021 atataaagag agaagtgata aataggacac aaaatttgga aagtaggtaa gtcactatga
    46081 gagaactaac tttatcttct aaggaattag aaaagaaaac tgctttatta aacgaaatat
    46141 ttagtgctga ttgtataatc caaagtttaa aagaaagttc attttcaaaa ctaacagatc
    46201 aggaaaaaat ttcttatatg ctaagtttag ataatctagg aactaaatat gatgaatcag
    46261 caagtagcga taagcttttg attgaattag tatcaagtct tagtagtttt ggaaaaatga
    46321 aaagatttaa tgtgatatta gaaatttttg cttggttaat cccttcattt atttggtttt
    46381 tttatatttt catagttgaa tctgagttaa tagccaaggt ctctcttgga ctgttaattc
    46441 cgatataaat aggttcagca atttacttta ataacttgta tacacctatt caaataaaaa
    46501 agcagttatt acatgaagga ttaagtggta atatgttaaa aagaattgca tgggataaag
    46561 ataataaaac agatattgag cttaaagaat ataaatacaa ttatttctcg agatgtatag
    46621 atagaaagtt actgtttaaa aaagaaggat tagaatttag ttttgatgaa gatgaatttg
    46681 aaaaattagt gatggactgg cgtaagaatt tactggatac ggtacaattc taaaagcaat
    46741 tgaggtcgaa attccgatac ttgaatcatg atctatatta tcgtgttatg agtgaaattt
    46801 ctaataaaga taagatattt catttggaca gtcatgtgaa tatgagctta acaattaaac
    46861 gtatttatga tcttttatat gtttataaac attaatgagg tgctaaaatt gacaacaata
    46921 actatgacaa aaaaaataga agtatatgtt gatggatcac aagaagaaag ggattccttt
    46981 tttgattttt tttggaaaaa tagtagagaa tatgtaaaag cgttgaatct ggtatataaa
    47041 cagctaatgt ttgaagagtt gataaaagat gcaattaaag aaatggatca aggttatgct
    47101 gagagagaag aaagtattca aaatcggatt gaaaagttag catatactga tccaatagat
    47161 caaaaaaaac ttgataaagc aaaagctgaa ttatctaaat tgagaaatga aaaaaataaa
    47221 gaagcgagag aaatattaac gcaagcgata gggttgaaga gtcaaacgag aacacgagat
    47281 gtagcaaaaa aattagactt tgactttgga gattttgttg attccgcaaa tatgaaagct
    47341 agtaaagatt ttggcaatga ttttatcgga attgtgaccg gacaaagaac gcctagaata
    47401 tataaaaata gaaacaaata tggattatta ccttttagag gtcgagatat taaaatccat
    47461 aaagatgata agttttatat ttatttacct aaaggcttta ggtttaaagt gaatttaggt
    47521 agcaagccaa gaaaagcaag agatgttgct tcagttttac aacacattat ggatggcgat
    47581 tacaaattaa atcaaagtaa tcttattttt gatggaagaa aattattttt taatctaaca
    47641 atttcttttg aaaaaagcta taatgaaatg gaattcaaga aaggtaatga attgatcgta
    47701 aactttgcag atttgtgtag cggtgaatgt ttaattaatg ataaatctgt aatgtctttt
    47761 ggtgatagcc aatatataag aaacttcaaa agtaaaattt tagcaatgaa atcaaaagaa
    47821 caaagccgag gagtcttttc taagggcggt catggtcgaa aaagaaaatt agatcaagat
    47881 cgatgggaag caataaagaa aagagaaagt aattttgtta aaacttataa taatcaatgt
    47941 tctgcaaaaa tcgttaaaac tgctattcgc aataaagctc aaattattcg tatcattgaa
    48001 ccagataagg cttatggcga atgggcttac tatcaattga aagaacaact agtaaacaaa
    48061 ggaaaaagat atgggataga agtcagaagt tagtaaatta aacaatcgtt gatttggcaa
    48121 cgaaatagtg agggaaaatc cactcactga agggtaaact ccgtcaagtt gtgtgaccag
    48181 acggaaggac gaatgttgtt gaggttttag aaccatataa aacagcatgt ttctcaaatt
    48241 tttaggataa gggaaattgc tagcagtttg ttttagaacc atataaaaca gcatgtttct
    48301 caaatgagta ctttaaatta attgatggcg ttcctgcttt agaaccatat aaaacaacat
    48361 ggatttaaaa caagaagtaa gtcagttctc cttttacttt agtaaggaaa aataaagtat
    48421 tctcaataaa acttaaaata agcaaacctt tgttttaaaa tgattgtcaa gttaaaaaaa
    48481 tctgggatca tataaaatag tatagttctc aaatatgatt agaaacccta ttaaagagca
    48541 atattaagcc aacttactta aatcgtttaa ttaagaacaa taatctcttt tagtcagact
    48601 tttagtctga cttttttgta tttccaaaat ttattcttta caaaaacgaa catacgtttg
    48661 tataattact tgtgaatggg ggaataaaaa tgtttgatta cagtagagaa ccggtgaatg
    48721 actatttttt aatcgacatg aagtctttct atgctagcgt ggaatgtatt gaacgaaatt
    48781 tagacccaat gactacaccg ttaattgtca tgagtagatc ggacaacact ggttcaggtc
    48841 tgactctagc ttcttcacct actgcaaaga aaaaatatca tattactaat gttagtagag
    48901 ctactgattt accttatcca ttaccaaaag aacttcatat tgttccacca cgaatgaact
    48961 tatatataaa aaaaaatatg gaagttaatc agatttttag aaagtatgtc gcagatgaag
    49021 atttattgat ttattcgatt gatgaatcaa ttttaaaagt gacaaaatcc ttaaatttat
    49081 ttactatata aattgagtta aattgtattc gtttttcctc ctataggcgt atactaaaga
    49141 aaacacttga ggagtgggga acatggaaac agaacgccaa atccgtgaat tggaaaaagc
    49201 gatgaatacg acagacaacc gccggttgta cgagcgtttc ctggcagtca agttggttct
    49261 tgaaggccac acccggaaag aagccgggca caccatcggc cgcgacgagc atactgtcag
    49321 ccactatgtg aaaaactact gccataatgg aatccaaggt ttagtctcca aaaaacaaag
    49381 cggacgacct tcacggctga cagatgaaca ggaagcggaa ctggtccaaa tcgtcgccta
    49441 ccacactccg gaggaagtag gtttcaaatc ccgtgccaat tggacattgg cgattgtgtg
    49501 tgacctgatt ctccgggaat ggagttatgg atatacgcaa aaaggcgtgg cggacctcct
    49561 tcaccggcta ggactaagct ggacccgtcc gacgtatacc ctcaaaaaag cggattccaa
    49621 gaaacaacag gtatttttgg aagaaacctt cccgagccta aaaaaaaatt gctgaatagc
    49681 gatattgacc acctcctatt cgaggacgaa accatgatcc gggattacca agccattggc
    49741 tgcacctggt ttctccgcgg ccaccagcga aaaatcccca cttatggaca acacaaaggg
    49801 gtaaaattga taggcacctt ggattatgaa accggtcgga tcatttgtac agaagaaggg
    49861 gcatacgacg cagcggcctt cctccgtttt ctggagcgga cgttgcggga ataccccacc
    49921 ggaaaaatcg tgatggtatt ggataatgcc cttatccacc acgccaagct gatccagtct
    49981 tttcttgaag aaaacgctag gcgcctggca ctcatcttcc tccctcccta cagccctcag
    50041 ttgaacctaa tggaaggcgt ttggaagtgg ctcaaggaat cggtcatcaa caatgttttt
    50101 tttgaccacg tccagaaaat caaacagtcg gtccgcggtt tcttagccga tgtaaacgag
    50161 cgtccccttg aggtgattga ccgactgtgc gtgcggatgt aaagtattta attcaaccta
    50221 tatagttcag gaacaagaag cgagagaaga agagaattag ctaaaataat caaagatgag
    50281 attcagaaag aaacaggtct aatagcaaca gtcggaatag gagataaccc attattagct
    50341 aaacttgcac tggacaatga agcgaagagt agtaaagaat ttatagctga gtggacttat
    50401 gagactgtac ctgaaaaaat atggaaaata aacgagatga ctgacttttg gggcattgga
    50461 tcacgcatga aaaaaagatt gaatcatatg ggaattatga gtatagaaga tttagcaaat
    50521 tggaatccct acacaattaa aagtcagtta ggtgtaatcg gactccagtt atatttccat
    50581 accaatggaa ttgatcgtac ggatattgct attccaccag aaccaaccaa agaaaagtct
    50641 tatgggaatt cacaagttct accaaaagat tatacgagga gagaagaagt agaactagta
    50701 gtcaaggaga tggcagagca agtagccatt cgaattagac aacacaattg caaaactggt
    50761 tgtgtacgct tagcaattgg tacgtcaatt ttggaagaaa aatcaggttt ctcgcaccag
    50821 atgaaaatac caattacaga taatacgaaa gaattacaaa attattgtct ttttttattc
    50881 aataagtttt atgaggggca ggaagtacga catataggag taacttatag cagattagta
    50941 tacacagatt ccctacaatt agatttattt agtgatccaa gaaaaaagat tgctgaagaa
    51001 aatttggata ggattattga taagattaga caaaaatatg gatttacagc tattatacat
    51061 gcttcatcta tgttagaagg tgcaagaagt attacgagaa gcactttaat aggaggacac
    51121 gcaggaggat taggaggttt gcataatgat tgaatataac gatagaggac gtttaaaatg
    51181 gtcgggattt tatttgtctg atcatactga aagaatcaat gcagacaatg tacaacgtgc
    51241 taatcataat ttggcaaaag aacaaatgac gactgaagag attgcgaaaa tactgagaac
    51301 tgctgtcata aaaaatagac ccgtctgcat tcaaaaagag gaacgaaacg ctgaaggtta
    51361 ctatccagat gatgtagtag gaaaaattat tggatatgac gagcttggtg tatttgtgga
    51421 agaagaaaaa gttcactatg atgaaattag gaatgtttct ttctcggaat tattaaaatg
    51481 gagtcagata tgaacattat taaaaaatac gaattcggtt atattacgta tgaacagcta
    51541 atagaggaat tatggggata tggtcaacaa ttaattaatg aagtggggat tgattgcttt
    51601 tgtttctata ttgaatccgg agcaggatac cataagaaca gatactatat atttccatat
    51661 ccaccaaaat agtctcttag atatttttaa attttctcca aatactttca attattgtga
    51721 aatatttccc tttaattaat caaatttttt ataaaagtaa tatataaaat aaattgcttg
    51781 aagtattata tttagatatc ataatttatg gaagaaaaat taattaaaaa ggatgagatt
    51841 ataatggggt tactggttcc gagagctaat acttatacta tacctttaac gaaacgccaa
    51901 ttaattgcta aaagatatca aaggattact agagccatca atagagagtt ttggaattct
    51961 gaaagtgata ctgctcacag tttatacgta ggttcttatg ggagaggaac ggcaattagt
    52021 actagtgata tagatataat agttgaatta ccgatggcag aatttgacag attcaaaaat
    52081 tatttatcaa atggcccgtc taagttatta caagttataa aaaacgcatt tcaggaaata
    52141 ctaccaaata gtgatattag agctgatgga caagttgtta aaataaattt ccatgatgga
    52201 atcaaatttg aaatagttcc agcttttaat gaaaaagatt attggggtga gtcaaaaggg
    52261 tttatttatc cagattcaaa catgggaggg aactggaaag ctacaaatcc caaaaaagag
    52321 caagaagcca tgaaattaaa gaatacaaaa agtaataatt tattatatgc tacgtgtaaa
    52381 cattttcgac atgtacgaga tacagaattt acgagttatc atctttcagg aatagtaatt
    52441 gacagtttcg tatatgaggc tatgggaaat tggaaattcg ttgaaaataa cagtgggggt
    52501 caaaacatta gttctgtatc ttatgagaca gctttattag aatactataa ctcacataaa
    52561 gttatgggcg gtttaaattt atactctcct ggaagcaatc aatttgttaa ttctgatagt
    52621 agtatcatat gtttagaaaa agtattaaaa aaaatagctt tataaagttt tatggaggaa
    52681 caacatggga gacgaaaaaa agcgcttgga agttcaatta cgtgaagcat atggacgagt
    52741 aacgtatact tacaccactc atttaaagca agccgataat attatttctg acaataaaat
    52801 tttaaggtgt cttcaaattg gtttgtcagc ccttactact ggaggggtac ttagtaccat
    52861 tattactgat aatagtgagt taacctttgt tgctggatta tgttcaacaa tttctcttgg
    52921 attaaactta tactttaaga attttgatct tgctgaagaa gctaaaaatc atcaaattgt
    52981 tgccgataaa ttatggttga tcagagaaga atacgtaagc ttactaacag atttaaatcg
    53041 tttaagtgtt gaagatattg tgaaaaaaag agatcaatta atgaacgaaa ctcatgaagc
    53101 atataaaaat gcccctaaaa caaatagtaa aagctatgaa tcagctcaga aagcattaaa
    53161 atctgaagaa gagcaatttt ttacagaaga ggaattggat aagatgttac cagagcatct
    53221 tagaaaaaag agttctaaat attgaactct ttttttatgc ttaaaattct cttgtaattt
    53281 ttctattcat ctatatttaa gaagaaatat gatgatagaa acatctccta aagccaccgc
    53341 aagccgttct aagcatagct tagcccgaca ttgctagata tgctcatctc accatgaacg
    53401 tttcctaaag ccaccgcaag ccgtcctagc cattgcctag tccgacatcg ctagattagt
    53461 gcatctcgcc ataaaagttt tctaaagcca ccgcaagccg ttctaagcat agcttagccc
    53521 gacattgcta gatatgctca tctcaccatg aacgtttcct aaagccaccg caaaccgtcc
    53581 tagccatcgc ctagtccgat tttgcttgat cggttccatt tctgtttatt tagtgggtca
    53641 acaccgtaat cttaaatagc catcgcctat ttaagattac taggtctgct ttgaaggtaa
    53701 aaagtctttc attcttcaag acttttaggc tttcaagcta cgcctgaaag cattgaaatt
    53761 catcgctctt tatttggttt tgccatttat aattccttac gcccattatc tttttcgcct
    53821 gtcatacctt gtcagaagtg tttagagctt atctcatctt tggattaata caagtggcgt
    53881 aaattttttt ctaaacgcaa gcgttacgaa aataatttac ttaaacactt gtattaatcc
    53941 ggcagataag aacgcattct aatcactccc gacaaggcaa gacaggcgaa aaagataaat
    54001 aaatgaacga aaggaagaaa cataaaatgc aaaaccaaat agcgatgaag aaaaaaccac
    54061 ctccctctaa aacaatccaa acaaaaaaga attttgaaaa accaaaaatc aaaaacagaa
    54121 agaagcgttc aacatgagag atagaatttt agaagttaat caagaaatgg taaacaagtt
    54181 aacaaatatc agtgggaact atgaaaagta cgatggtact ttacttgata actatatttt
    54241 tcacgataca caaaatatca gaattggcag aagtaaaccg agagcgttca ttctcatcaa
    54301 agagaaatat ttgaatgaat ggaatagcac ctatgaaatg gttatgactg ataactttga
    54361 aaaggtattg gatttcagaa atgctttgtt gggttctgat atgggaaata gcagtcttga
    54421 atctattgaa gttgaaaaca agaccgagaa tgaaggtgaa ggattgatta agaatgtctt
    54481 gaaaatcgaa caaaaatata tcagtgaaga tagaagtttt tatagagtca caagctcggt
    54541 tggtctagca aactttgcta ctaacttaat tggaaacaat gctagtgaat ctttgctagt
    54601 gatgtgtctg aatacaaaga atgaggtcat tgcttatagc gaagttttca aaggtggaat
    54661 taatcaatca gtagcgatac caagagagat ttttcaacta gctttgctaa acaattcagc
    54721 aaaaatcgca gtatcacata atcatccgag cggatatgaa tttccaagtg aaaatgacaa
    54781 aagttttact acgaatttga aaaacgcagg aaaaacaatt ggtattgaat tgttagacca
    54841 tatcatcgta acaagtaaaa acacaagcta ttactcattt caagaaaatg gttctttata
    54901 aaaattagag aaaagaggaa aacaagatgg gcgaaagaac acaagcgtta attacagtca
    54961 aatatggtaa cagcaaaacg aaagcaagcg tgtcgattca ttatcagtgg gggtatggtc
    55021 gatatatgct gatggacgta ttgaatcaag gaatttattt aatcaataaa gcatacgact
    55081 tacgagataa atcaagagaa gaattagcaa aaggaatctt tgatagaagt acaggagtta
    55141 tcaattttgt cacctatgaa tctggagtga gtgcttatga tgaaacaggg tttggtgggc
    55201 gctatgaacc ttatcaagaa acgattgaag atgtgatgaa gttttcagat aacaatgatg
    55261 gctatgctca tttagagatg gtgttcgatg aaaatggatt tagtctagat gataaaacca
    55321 gattgcgatt gtttgatttt gagggtcaac agataggact aaacaagttt tgtgaaagcg
    55381 gaaaagagtt tgctacgaaa gattttatta agggatacag attattgcta gaaaattatg
    55441 gaataaacct agaagaagag agcgtgaaat aagtgtttga aaaaagaaaa gaccagttcc
    55501 ctaatttaga agcgaaaagt caaaaagtga ttgatggtga aatgattgaa ttattccaaa
    55561 aaagttatgg aaccatagaa actaccgcaa gttgcaaata ctactttcaa ggaaagagaa
    55621 acacgtttaa aggctttgcc gattctgatg aagaattggc aaagaaaatc attgattggc
    55681 ttgtcagcag tgaacacgta gcaagagaag ataccgttag aacaacgatt aatggggaag
    55741 tcctaacagg tactagatgt gtttgttgta atcatgaatt tgtagaagat gaaggttgct
    55801 accgaatcaa tgaatatggt gattttattt gccagagttg ccacgagata gaatttccaa
    55861 ccgaaagaag ttggtttgaa tacatgctac aagattatga gatggaaaca gaaaatggtg
    55921 aagtagtttt ttgtaccaaa gaggaattga gtaagagaag cgatgatgaa attgaagatt
    55981 ggtgtgtgtg gttagtaggg acagatgatt gcgttgcata ttacacaaca gcagaatagg
    56041 agtgtgaatc agcatggaag gaatggattt tatagatcat gaagatttga ttgattttgg
    56101 ttatacatgg aaaggaatgg taggtattag cagaagtctt gctaatgcct tttacgaaag
    56161 aaattatgca gtctatgtcc tttatgatga caatacagaa agccttgttg atgaagagta
    56221 taaattggat ttagagaatg ttttatatgg gatagaaaaa gaagatttag caaaatatat
    56281 tttttcatgg ttgggtcaat aaacgaaaga gggtagcaac atgacaaaat tcaaaagtaa
    56341 aggtatgcaa tggtgttctt attattatgt tgggaatgat gaagagcttt tgagaaaagc
    56401 caaacaaaaa ggttatccag ataagatgtt aaaacagatt ggctattaca tgccagaaga
    56461 gagaatagaa aaagaatctt gtaaggttct ttttgatagt gaacagctat tagttttgtt
    56521 aactcaaaat aaattgattt gtcaatttga aaataattaa tatggggtga agaaccatga
    56581 atgagaataa gatggtatta agtgaatacg caagattgag aaccattaat tggttgaatg
    56641 gtatgcaaag tagtggcgct tttgatggat ttgatgagga gaatatggac tttgaaagtt
    56701 tatcgaatga ggatattaga tattatttta tcgaatggta ttttacagaa ccagatgaag
    56761 aggtggaaga tgaaccagaa ttaattttag aacgtcaaag aattttattc agtgaacaag
    56821 atagtaagag gttgtatggt ttatatgact tacaactgaa aggattaaaa gcgcagtatg
    56881 ttgctgatag ttacgatgat atggtagaac agggggcaga tagggttgtc acttattcag
    56941 aagatgatga agaggaatgg gaaaactatt cagatgatga gatatgcttt aatgatgggt
    57001 atatgcctat tgaaatcacc gaagaagaat atgataaagt gtcggatagc gatgagattg
    57061 gtatgtgtac ggttgtaagt ggattggata tagatgtcca aagagtagag gttgcatacc
    57121 agaagatgaa agaacgtata aaaagtaaag tagaatcttg atttgattgg attgtttgca
    57181 aagtagctaa ttaagagtat aatataatca taaatgtagt taatggatat aataatgagt
    57241 atattatact caaaagaggt gaacgaaatg aaggtaaaaa atccaaaaga aaaaagcaga
    57301 attcaagtcg gaatagacaa ggatttaaaa gaaaacgcag aaatgatatt ggaagagcta
    57361 gggttaaatc caacaacagc aatcacaatc ctttataagc aagtggtcgc tagaggtgag
    57421 tttccagtag agataaagct aagcgaagag gaaaagcaag gaatcagatt gcaacagcta
    57481 actatggata tgccagttga tgttctagat acagatgaaa agttagaaga gtggttcaat
    57541 gaagcctaat gagatagtaa cggtctatgt tgcatttaca gataaaaatg gtggtaaaag
    57601 aagacctatt ttggtggtta gtgataaaga agatagagta gaattttttg gaatcacaag
    57661 tcaatatgag aataaatcag accaaataaa gagagtgtat tttcctattg cagaatggga
    57721 aaaagtaggt ctaaagaagc aatcatggat tgacgtaggg tctttaaagg ctattcccaa
    57781 aagccgagag aacatatcat ttaaaaaggt tggtatgcta tcagctattg atacgaaaaa
    57841 gctaaatagg tttatcgaga gattacaaag aaaagtctaa caaggctttt ctttttttgt
    57901 ttatatgtat attaagatat acgtttatat aaacgtattt atgtatatta aaatacacgt
    57961 aaatatatac gtgattataa aaattaagaa aatattaagt attgttaaat agtgataatt
    58021 agtaatatac gaattataag atacatttta tatttaatgt atgtaaataa ataaaatgac
    58081 tgacggatat tgaatagtaa taaataacaa taattcagac ggatacagtg agtgaagtag
    58141 cttgctacga acgaacaggc agaatgaaac ggtagtttca ttagagcaag tcgcttgcga
    58201 cgtagcgtta ccactgatgt aactggcaag ttacatagag agagtttgct tgcaaacgaa
    58261 cgttacccac ttgaatagaa aacaaaaata acatattagt gaattttgaa taggagccac
    58321 aactcaacca accgcttgcg gtgggtgagt cccacggagg aaaagacgaa cgaacg
//
LOCUS       DACJGB010000010        55502 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.010, whole genome shotgun sequence.
ACCESSION   DACJGB010000010 DACJGB010000000
VERSION     DACJGB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.010"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            558..3197
                     /locus_tag="IYI34_000675"
     CDS             558..3197
                     /locus_tag="IYI34_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR0558567.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(3365..4063)
                     /locus_tag="IYI34_000676"
     CDS             complement(3365..4063)
                     /locus_tag="IYI34_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAR0558568.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            4281..5426
                     /gene="tgt"
                     /locus_tag="IYI34_000677"
     CDS             4281..5426
                     /gene="tgt"
                     /locus_tag="IYI34_000677"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAR0558569.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            5523..5903
                     /gene="yajC"
                     /locus_tag="IYI34_000678"
     CDS             5523..5903
                     /gene="yajC"
                     /locus_tag="IYI34_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAR0558570.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            6208..8805
                     /gene="adhE"
                     /locus_tag="IYI34_000679"
                     /gene_synonym="adhC"
     CDS             6208..8805
                     /gene="adhE"
                     /locus_tag="IYI34_000679"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAR0558571.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            9034..10140
                     /locus_tag="IYI34_000680"
     CDS             9034..10140
                     /locus_tag="IYI34_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAR0558572.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            10157..11185
                     /locus_tag="IYI34_000681"
     CDS             10157..11185
                     /locus_tag="IYI34_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAR0558573.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLEGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            11191..11670
                     /locus_tag="IYI34_000682"
     CDS             11191..11670
                     /locus_tag="IYI34_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAR0558574.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            11686..12981
                     /locus_tag="IYI34_000683"
     CDS             11686..12981
                     /locus_tag="IYI34_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAR0558575.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            12992..13816
                     /locus_tag="IYI34_000684"
     CDS             12992..13816
                     /locus_tag="IYI34_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAR0558576.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPETP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            13981..14880
                     /locus_tag="IYI34_000685"
     CDS             13981..14880
                     /locus_tag="IYI34_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR0558577.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(15006..15875)
                     /locus_tag="IYI34_000686"
     CDS             complement(15006..15875)
                     /locus_tag="IYI34_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR0558578.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            16028..17488
                     /gene="rhaB"
                     /locus_tag="IYI34_000687"
     CDS             16028..17488
                     /gene="rhaB"
                     /locus_tag="IYI34_000687"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAR0558579.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            17485..18741
                     /gene="rhaA"
                     /locus_tag="IYI34_000688"
     CDS             17485..18741
                     /gene="rhaA"
                     /locus_tag="IYI34_000688"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAR0558580.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            18755..19600
                     /gene="rhaD"
                     /locus_tag="IYI34_000689"
     CDS             18755..19600
                     /gene="rhaD"
                     /locus_tag="IYI34_000689"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAR0558581.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            19602..19913
                     /gene="rhaM"
                     /locus_tag="IYI34_000690"
     CDS             19602..19913
                     /gene="rhaM"
                     /locus_tag="IYI34_000690"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAR0558582.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            19995..21146
                     /gene="fucO"
                     /locus_tag="IYI34_000691"
     CDS             19995..21146
                     /gene="fucO"
                     /locus_tag="IYI34_000691"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAR0558583.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEVSVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            21273..22031
                     /locus_tag="IYI34_000692"
     CDS             21273..22031
                     /locus_tag="IYI34_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAR0558584.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            22131..22775
                     /locus_tag="IYI34_000693"
     CDS             22131..22775
                     /locus_tag="IYI34_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAR0558585.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            22782..23789
                     /locus_tag="IYI34_000694"
     CDS             22782..23789
                     /locus_tag="IYI34_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAR0558586.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            23805..24647
                     /gene="kduI"
                     /locus_tag="IYI34_000695"
     CDS             23805..24647
                     /gene="kduI"
                     /locus_tag="IYI34_000695"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAR0558587.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            24667..25470
                     /locus_tag="IYI34_000696"
     CDS             24667..25470
                     /locus_tag="IYI34_000696"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAR0558588.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25719..26783)
                     /locus_tag="IYI34_000697"
     CDS             complement(25719..26783)
                     /locus_tag="IYI34_000697"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAR0558589.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26780..27865)
                     /locus_tag="IYI34_000698"
     CDS             complement(26780..27865)
                     /locus_tag="IYI34_000698"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAR0558590.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="IYI34_000699"
     CDS             complement(27878..28855)
                     /gene="mvaD"
                     /locus_tag="IYI34_000699"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAR0558591.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="IYI34_000700"
     CDS             complement(28848..29792)
                     /gene="mvk"
                     /locus_tag="IYI34_000700"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAR0558592.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            30114..30767
                     /locus_tag="IYI34_000701"
     CDS             30114..30767
                     /locus_tag="IYI34_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAR0558593.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            30852..31757
                     /locus_tag="IYI34_000702"
     CDS             30852..31757
                     /locus_tag="IYI34_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAR0558594.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(31759..32391)
                     /locus_tag="IYI34_000703"
     CDS             complement(31759..32391)
                     /locus_tag="IYI34_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAR0558595.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            32711..33235
                     /gene="infC"
                     /locus_tag="IYI34_000704"
     CDS             32711..33235
                     /gene="infC"
                     /locus_tag="IYI34_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAR0558596.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            33307..33507
                     /gene="rpmI"
                     /locus_tag="IYI34_000705"
     CDS             33307..33507
                     /gene="rpmI"
                     /locus_tag="IYI34_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAR0558597.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            33560..33919
                     /gene="rplT"
                     /locus_tag="IYI34_000706"
     CDS             33560..33919
                     /gene="rplT"
                     /locus_tag="IYI34_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAR0558598.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            34171..35508
                     /locus_tag="IYI34_000707"
     CDS             34171..35508
                     /locus_tag="IYI34_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAR0558599.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            35556..37685
                     /locus_tag="IYI34_000708"
     CDS             35556..37685
                     /locus_tag="IYI34_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAR0558600.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            37660..38349
                     /locus_tag="IYI34_000709"
     CDS             37660..38349
                     /locus_tag="IYI34_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558601.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(38757..39370)
                     /locus_tag="IYI34_000710"
                     /pseudo
     CDS             complement(38757..39370)
                     /locus_tag="IYI34_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(39506..39700)
                     /locus_tag="IYI34_000711"
     CDS             complement(39506..39700)
                     /locus_tag="IYI34_000711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558602.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(39750..40190)
                     /locus_tag="IYI34_000712"
     CDS             complement(39750..40190)
                     /locus_tag="IYI34_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAR0558603.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(40194..41039)
                     /locus_tag="IYI34_000713"
     CDS             complement(40194..41039)
                     /locus_tag="IYI34_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAR0558604.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            41188..42606
                     /gene="pepV"
                     /locus_tag="IYI34_000714"
     CDS             41188..42606
                     /gene="pepV"
                     /locus_tag="IYI34_000714"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAR0558605.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(42663..43610)
                     /locus_tag="IYI34_000715"
     CDS             complement(42663..43610)
                     /locus_tag="IYI34_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR0558606.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(43833..44183)
                     /locus_tag="IYI34_000716"
     CDS             complement(43833..44183)
                     /locus_tag="IYI34_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR0558607.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            44383..45462
                     /gene="pepA"
                     /locus_tag="IYI34_000717"
     CDS             44383..45462
                     /gene="pepA"
                     /locus_tag="IYI34_000717"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAR0558608.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            45606..45926
                     /locus_tag="IYI34_000718"
     CDS             45606..45926
                     /locus_tag="IYI34_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAR0558609.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            45948..46412
                     /locus_tag="IYI34_000719"
     CDS             45948..46412
                     /locus_tag="IYI34_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR0558610.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            46617..47222
                     /locus_tag="IYI34_000720"
     CDS             46617..47222
                     /locus_tag="IYI34_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAR0558611.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(47261..48550)
                     /locus_tag="IYI34_000721"
     CDS             complement(47261..48550)
                     /locus_tag="IYI34_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAR0558612.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            48978..49457
                     /gene="rlmH"
                     /locus_tag="IYI34_000722"
     CDS             48978..49457
                     /gene="rlmH"
                     /locus_tag="IYI34_000722"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAR0558613.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            49542..50051
                     /locus_tag="IYI34_000723"
     CDS             49542..50051
                     /locus_tag="IYI34_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAR0558614.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            50151..50801
                     /locus_tag="IYI34_000724"
     CDS             50151..50801
                     /locus_tag="IYI34_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558615.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            51252..52190
                     /locus_tag="IYI34_000725"
     CDS             51252..52190
                     /locus_tag="IYI34_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAR0558616.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            52203..53255
                     /locus_tag="IYI34_000726"
     CDS             52203..53255
                     /locus_tag="IYI34_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0558617.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            53521..54156
                     /locus_tag="IYI34_000727"
     CDS             53521..54156
                     /locus_tag="IYI34_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAR0558618.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFIGTSKTLAILAVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            54347..55138
                     /gene="msrA"
                     /locus_tag="IYI34_000728"
     CDS             54347..55138
                     /gene="msrA"
                     /locus_tag="IYI34_000728"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR0558619.1"
                     /translation="MLDVEQMEIDLYNIILNRNTREWERTQFEEAKEDLAKMSIKQVI
                     EKLEVNLRPLALRDNLTPDAMDFYLRITGESASAKAYDYAKHKITSPLYQERAIFAGG
                     CFWCMVEPFETKPGVISVLSGYTGGETKKPNYDQVSGGYTGHVEAVEIIFDTRQITYE
                     ELLEIYWQISDPTDAFGQFQDRGKQYRSILFVVNEKQRQLAEKSREQLIQSNRFSRPI
                     VTEIRQAKTFWPAENHHQQFYQKQPKRYKKIKHSHQLHLFFQRHR"
BASE COUNT        17619 a         9922 c        11894 g        16067 t
ORIGIN      
        1 aaaaagccga gctctcgata agctcggctt tcttgtacct cggaaggatt caaataaaca
       61 gaaatgattg taactttcca tcatacatgc aaagcacggt gttgaatttc ttcatgttaa
      121 acaaaaattt tgttttcgtt acatcttcaa catttgaaac atctcttgtt cctattcaat
      181 tttcagacgt ttacgttatt caattgttac gtatcgcatt acttcttgtt tcggatactt
      241 cgtgatccaa acatccatac gcattcacta tttataggtt tgtactctac acgcatgtct
      301 gggtgaaacc tgtacttcta acaactttgt ttgcttacac tgttctatcg agaaattctt
      361 ctgtctattt gatggtatcc tccttcctta catctatata atagcatcct ttggtagcgt
      421 ttgcaaatat aagcacttta aattttacgc tttcttaaca aataatcgat aagcccttta
      481 tttgaagtaa ataaaaaaag gatgttatag taaaataagt accttttttt gtacgaagat
      541 tttaaaggag gaataggatg gaagaataca agcagacaat agaagaagta aagaaggtaa
      601 cagaagcaga tccagatact gggttgtctt cacaagaagt ggatgaacgg cgaaaaaagc
      661 agggattgaa taaattcgat gaggcgccga aagaatcgat gatcaaaaaa ttctttcgaa
      721 gcttgtccga ttttacaacg atcatccttc ttgtagcagc ggtgatttcc ttttataccg
      781 catttgctac agaacatggg gatttatttg aaggactgct gatcatcgct atcgttgtga
      841 tcaactcggt attggcgatt gtccaagaag gaaacgcaga aaaagcactg gaatccttgc
      901 aagatatgaa taaacaaacg gcaactgtca tacgagacgg caaagtgacc actgttgagt
      961 cagagcaact ggtagtgggt gatattcttg ttttagagtc cggtgatgct atttctgccg
     1021 atgcccgttt gatcgaagca tcacaattac gtgtagaaga atcggcgttg actggagaaa
     1081 gtgaagcagt tgaaaaagat gcagcattta ttgctgaaga agatgagtca ttaggtgacc
     1141 aacttaatat ggtatttaaa ggatgtacgg ttgctgcagg tcgtgggaaa gcgatcgtca
     1201 ctgccattgg catggctaca gaaatgggta agatcgcgga tcttttgaac gaaaatacta
     1261 tgcaaaaaac acctttacag gtccgattga atcagctagg caaacgaatc agtatgatcg
     1321 ctttagcggc tgcggcttta gtatttgtga ttggtgaact gcaaggagaa ccactccttg
     1381 agatgttcat gacttcgatt tccttggctg ttgcagctgt accggaaacc ttgactgtta
     1441 ttgttacgtt gacacttgca tacggtgtgc agaagatggc gaggaaacat gcgatcatcc
     1501 gacagttgcc agcagttgaa acgctaggaa ccgctaatgt tatctgctca gacaaaacag
     1561 gaacgctgac acaaaatgaa atgcgcgtcc gccgggtatg gagcaaagaa gatgaagtaa
     1621 cgaatatcga agattcaatg acgaacagcg cgatggaaat attgaaaatg gctgcactat
     1681 gtacagatgt gacggtcgag caagaagatg acgaccttgt tatcaaaggt aatccaacag
     1741 aagtagccat cgtaagggct gttgaagaga attatcatac aaaggcagaa ttagaagaaa
     1801 aatatccacg agtaaacgag cttccttttg attctgaacg aaaaatgatg acaacagttc
     1861 accaaatggg cgaaaaatac atctcaatca ccaaaggagc attcgatgtt cttgctccgc
     1921 gctttagttc aggcgatgta gaacaagcgg cgatcgttaa tgatcgcttt ggtaaacggg
     1981 cactgagagt gattgctgtt ggttatgcta cttatgacga agaaccgcaa gatatttctt
     2041 ctgaagcact ggaaaaagac ttacgtttga ttggtctgat cggaatgatc gatccaccac
     2101 gtccagaaag taaaggagca atcaagcgag cgaaaaaggc tggcatcaaa acagtcatga
     2161 tcactggaga tcatgtggtg actgccagtg ccatagcgaa agaattaggt atcttgaatg
     2221 atccgtcaga agcactttca ggatcagaac tgcatcaatt atcagacgaa gaattagatg
     2281 cgcgtgtcaa agctctctct gtctatgctc gggtaacgcc agaagacaag atccggatcg
     2341 tcaaatcttg gcaacgtacg ggtgcagtcg tagcgatgac tggggatggt gtgaatgatg
     2401 caccagcgtt gaaagcaagt aacgttgggt gtgccatggg gatcacagga acagatgtgg
     2461 cgcaaagtgc cgcagatatg attttgacgg atgacaactt tgcgacgatc gtagacgctg
     2521 tcagtcaagg acgttcggtt tatcagaata ttcgtaaagc tatcaacttt ttgctaagct
     2581 gtaatatttc ggagatcttt attgttttga ttgcgatgct gttaggatgg ggagcaccat
     2641 ttacagctgt gcaactactg tttgtcaatg tagttgctga tggacttcca ggatttgctt
     2701 taggaagaga gccagcggaa cacggcatca tggatcagcc acccattccg aaaaatgaag
     2761 ggatctttgc tcgaggttta ttgcaaaaaa ttgccataaa tgcaggggtc ttcaccatcg
     2821 taaccttgtt tggttattat ttaggtagtt atgtcgatac tatcagccca tgggtagacg
     2881 caagtcagca tgtcggtcaa actgttgcat tcttgatctt agcttattca tcgattctgc
     2941 atgtgttcaa tgtacgcagc agccaatcga tcttcaaagt caatttagcg accaataaag
     3001 ctttggtaga aatggcgtta ctggcattag ctattacaac ggctgttgct ttgctgccat
     3061 ttacgcaaga actattcggc ctagtacaca tcagtttgaa tcactggttc ttggtaggga
     3121 ttttatctat cgtaccaatc gcggtgaatg aactgatcaa gttccatcga ttaccggaag
     3181 cagaagaaga ggaatgatta actgtttcta agttgatttt ctttttactg gaactataca
     3241 aaaaggaggt gtgacatgaa tgttgtcaca cctccttttt gctaatgggc tgaatgtttc
     3301 ttttactgcc tctcgataaa cggtgttctc ctgccacccc ctcggtcaca agtcacactc
     3361 tcttttaaat atcttttggc aattgttcat aaaatgctgc ttttgttgca gaaatgtatg
     3421 ggctgagcca tagataacca ataccagcag ttgctaatgc taagatatgc cagccgataa
     3481 atgaaagatc taaccagaaa agttgtcctt tataaccatc catcaatttt cgacttgcag
     3541 tgatcgtatc cagtacttta ggtgtttgtc ctgtttcttg gacgatatcg tagtaaataa
     3601 aatagctttg agaataagaa tagcttttaa tgattccagg aataactaat agcaaacccc
     3661 ataatgttgt aaagatcgag atcagcacag ctaagctgat cactccgccg ataaacaaac
     3721 ctttgaatcc acgaaatgca tctttgaatg gttcgatctg atctttttgt ccacgtaaaa
     3781 tatcaagata agtccaagaa ataccgctca tgaacaaagc gctaatgatg ctggaaataa
     3841 ttccgccacc gcctcctgaa ctaccgcctc ctgaagttgt cgcttctgag acgctcgctg
     3901 atccatcata tatggtggtg gcaaacaaga taataggaat cataataaga agaatagtag
     3961 caataccaat caatgtaggg attagattta gtaaaacagc ttgtccccaa cgtcctttta
     4021 gactctcttt tgcttggcgt tttaattctg cagaagattg catgattttt ccctcctttt
     4081 ctttaataac accaatatac catgtatcaa aaaatatttc tgtcataatg tttttctttt
     4141 ttttgttatt cataaaaaaa ggataaaaaa aagaatatta aataaagtag aaggaagccg
     4201 agggaacttg caaaaagcac ttgaaacgat tatgatggtg ggagttgtca agatatttta
     4261 tatagaaagt agggaaatga atgacgcaac ctgccattcg ctatcgttta atcaagaaag
     4321 aaaaacatac tggcgcgcgt ttaggggaat tgatcacgcc acacggaact ttccccacac
     4381 caatgttcat gccagtagga acattagcta cagtaaaaac aatgtcccca gaagagttga
     4441 aagaaatggg tgctggcgtt attttaagta atacatatca tttatggcta cgccctggtg
     4501 aagacttagt agaagaagct ggcggactgc ataaatttat gaactgggat caaccgattt
     4561 taacagattc aggcggcttt caagtttttt cattaagtga tatgcggaat atcgaagaag
     4621 aaggcgtgca tttcaaaaat cacttgaacg gttcgcgtat gtttttatca ccagaaaaag
     4681 caatcaacat ccaaaataaa ttgggctctg atatcatgat gagttttgac gaatgtccac
     4741 cattcgatga aagttacgac tacgtgaaaa aatcagtaga acgtacgtct cgatgggcag
     4801 aacgaggatt gaaagctcat gctaatccag accgtcaagg attatttgga atcatccaag
     4861 gagcagggtt tgaagatctt cgtcgtcaaa gcgcgaaaga tctagtcggg atggatttcc
     4921 ctggttactc aatcggcggg ttatctgtcg gggaacctaa atcagaaatg aatcgtgtat
     4981 tagaattcac tacaccgttg atccccgaaa acaaaccacg ttacttgatg ggtgtcggtg
     5041 ctgctgattc attgatcgat ggtgttatcc gtggagtaga catgtttgac tgtgtcttgc
     5101 ctactcgaat cgctcgaaat ggcacatgta tgacttctca aggccgtcta gtagtgaaaa
     5161 atgcgaaata tgcccatgat ttccgtccaa ttgatgagaa atgtgattgt tatacatgta
     5221 aaaactatac acgtgcctat atccgccatt tgatcaaatg tgatgaaaca tttgggattc
     5281 gtttgacttc ttaccacaat ttatacttct tgttgaatct aatgaaacaa gtacgtcaag
     5341 caatcatgga tgataatttg cttgaattta gagaagcctt ttttgaagaa tacgggttca
     5401 ataaagaaaa cgcaaaaagc ttctaaagga aaatctttta aaaattaagt aaaaaactag
     5461 tgttatcagc atttttctgc taaagtgttt tttgtagata cagaattagt gaggtgaaaa
     5521 aaatgggaac attaccaatg cttgtgatgt ttatcgcgct attagcaatg tggtttttta
     5581 tgtcacgttc gcaaaagaaa caacagcaag aacgtcaaaa cttgttagat agcatgaaac
     5641 caggtgattc agtcgtaacg atcggcggat tacatggagt tgtttcagaa atcgacaatg
     5701 acaaacggac agttgtttta gactgcgaag ggatcttcct tgagtacgat cgtgcagcaa
     5761 tcaaaacggt caaaccaggg actgtcgtaa caaatgatgc aacgactact gttgaaacaa
     5821 cagaagaacc agctgagaca gttgaagtaa aaactgaaac aacagaaacg ctggaagaga
     5881 cagaagagaa ggaagaaaaa taagtatcaa agcttattta aagacgaggt agggcagaaa
     5941 gatgtcctac ctcatttttt attcaaaaaa gaaagagata agagcatggg atatattgct
     6001 caatattgaa taatcgggtg tgtcatataa ataataataa ataactttat atatttttct
     6061 atacaaacac ctgtaaataa agcaatttta tatttaaaaa taactgtgaa aaaaatcaca
     6121 aaaaagtgtt tacatagaaa aataaatatt gtatgatata catgtgaatt atttaacaaa
     6181 taaccatgac ccattaggag gataatgatg gctaaagtaa tagaaaaaga aaaaacgaag
     6241 acaattgacg tacaagcaat gattgatgat ttggcagaaa aagcaaatgc agcattgaaa
     6301 gaaatggaaa gctttgatca agaaaaagta gaccatatcg tgcatgaaat ggcaatggca
     6361 gctttggatc aacatatgcc attagcgaag atggctgtag aggaaacagg ccgcggtgtt
     6421 tacgaagata aagcaatcaa aaatatgtat gcgtccgaat atatttggca taatatcaaa
     6481 catgataaaa cagttggtgt gatcaatgaa gatgtccaaa aagggttgat tgaaattgct
     6541 gaaccagtcg gtgtggtttg cggagtaact ccaacaacga atccgacttc cacaacgatt
     6601 ttcaaatcaa tgatcgcgtt gaaaacaaga aatccaatcg tttttgcttt ccatccaagt
     6661 gcccaaaaat cttctgcaga agcagcacga gtggtacgtg atgctgctat cgctgccgga
     6721 gctcctgaaa attgtatcca atggatcgaa catccttcga tcgaagcgac ttctatgcta
     6781 atgaaccatc caggtatcgc gatcgtctta gcgacaggtg gagctggaat ggtcaagtct
     6841 gcttactcta caggaaaacc agctttaggt gtaggtccag gtaacgtacc agcttatatt
     6901 gaaaaaacag caaaaattaa acgtgcagtg aacgatttga tcgtttcgaa aacatttgat
     6961 aatggaatga tttgtgcttc tgaacaagct gtgatcgtgg ataaagaaat ctatgcagca
     7021 gtaaaagcag aattccaagc acaccaagtt tatatcgtaa aaccagatga attgcaaaaa
     7081 ttagaagatg cagtgatgaa cgaaggaaaa tatgcggtca atccatcaat cgttggacat
     7141 tctgcgatgg aaatcgcaaa attggcagga atcagtgtac caaaaggaac gaaaatgctg
     7201 attgctgaat tggaaggcgt aggcccagat tatccgctat caagagaaaa actttctcca
     7261 gttcttgcca tgatcaaagc taacaacacg gaccatgcgt ttgatttgtg tgaaggtatg
     7321 ttgaatcttg gaggattagg acatacagca gttatccatt cagaaaacga agagttgcat
     7381 gtcaaattcg gtcttcgaat gaaagcttgt cgtattttag tgaatacacc atctgcagaa
     7441 ggcggcatcg gtgatatcta taacgaaatg attccgtcat tgactttggg atgcggttct
     7501 tatggtaaga actctgtttc tcgtaacgtc tctgctgtaa acttaatcaa tgtcaaaact
     7561 gtagcgaaac ggagaaataa catgcaatgg ttcaaattac cgccaaaaat cttctttgaa
     7621 aagaactctc tattgtactt agagaaaatg gaaaacgttg aacgtgtcat gatcgtttgt
     7681 gaccctggta tggtccaatt cggttactgc gatacagttc gtgaagtatt ggcacgacgt
     7741 aaaaatgatg tgaagatcga agtcttctca gatgttgaac cgaacccatc gacaaatacc
     7801 gtatacgcag gaacgaaact gatggcagac ttcaagccag atacagtaat cgctcttgga
     7861 ggcggttctg caatggacgc agctaaggga atgtggatgt tctatgaaca cccagacaca
     7921 tctttctttg gtgcaaaaca aaaattcttg gatatccgta aacggacata taagatcgac
     7981 aaaccagaaa aaacacaatt cgtatgtatc ccgacaacat cagggactgg ttctgaagta
     8041 acgccatttg cggtcatcac tgacagtgaa acacatgtga aatatccgtt agcagattat
     8101 gcattgacgc cagatgtcgc aatcgtcgat cctcaattcg tcatgtcagt tccagcatct
     8161 gtcacagcag ataccgggat ggatgtgtta acacatgcaa tcgaatccta tgtttcagtc
     8221 atggcttcag attatacacg tggattgagc ctacaagcaa tcaagttagt ctttgactat
     8281 ttagaaaaat cagtaaaaac acctgatatg gaatcacgtg aaaaaatgca caacgcttct
     8341 acaatggctg gtatggcgtt tgctaacgca ttcctaggta tttgtcactc agtcgcacat
     8401 aaaatcggcg gggaatacgg gatccctcat ggacggacaa atgcgattct tttaccgcat
     8461 attatccgct ataatgcaaa agatccacaa aaacatgcga tgttccctaa atatgactat
     8521 ttccgtgcag atacagatta cgcagatatc gcaaaattct taggtctgaa aggcgaaaca
     8581 acagaggaat tagtcgaagc tttagcaacg gctgtttatg aattaggaaa atctgtcggc
     8641 atcaacatga gtctaaaagc tcaaggagtc acacaagaaa cactagatac aaccgtagac
     8701 cgtatggctg aattagctta cgaagaccaa tgtacgacag ctaatccgaa agaaccattg
     8761 atctctgaat tgaaacagat catcattgat gcttacaacg gataagcaaa agaaagcgta
     8821 gaaaaaagat aagaaaatag atgaaaaaga acttggaaaa cgttgtttct ccaagttctt
     8881 tttttaattt ccaagtaact gaaagttact tttgttattt aacatttagg tcaattttag
     8941 acagtttttg ttcaaaaagc gatatttata gtggacggta ttactgctat attaaggata
     9001 taaaatgaaa gcgtttaaaa aggtggcgta aagatgatta ttaaagaaga gatttttgta
     9061 cctaaagaat tagtgaaaga aaaaattgac ctgctcgttg aaaacctgac aacgatcaaa
     9121 gacgataatg gtgaattttt gttggacttt gatggactga aagtagatga caaaagctgg
     9181 acagtgtgga attggccaca aggtgtcggt ctatatggta tttataaaaa ttaccagatg
     9241 acaaaaaatc ctcgcgctta tcaagtggtc aatgaatggt ttgaggatcg tatggaagaa
     9301 ggcgctccgc ccaaaaacgt caatacaatg gcaccgctat tgactatggc ttacttgtat
     9361 gaagacacga aagattcacg ctatttgcct tatttagaac aatgggcaga atgggtcatg
     9421 aatgacatgc ctcggacaaa tgaagacggg ttacagcatg ccacatatgg accggaaaat
     9481 aagaatcagt tgtgggacga tacattgatg atgacagtgc ttcctttggc aaaaatcggg
     9541 aaactgttga atcgcccaga atatttagaa gaagctcgtt accagtttat gatccatatc
     9601 aaatacttga tggataaacg aacaggattg tggtatcacg gctggacgtt tgaaggaaat
     9661 cataactatg ccgaagcatt ttgggctcgt ggaaactgct ggttgacgat tgctattcca
     9721 gagatcattg aaatcttaga attatcgaaa gaagatgcat tgcgcaaatt attgatcgaa
     9781 acacttgaag cacaagtgcg tgcattaaaa acttatcagt ctgaaagcgg tttatggcat
     9841 acgttgttgg atgatccttc ttcttacgta gaatcttctg caactgcagg atttgcttac
     9901 ggtattttga aagcggtcca taaacgttat ttaccacaag aatataaaga agtcgcttat
     9961 cgtgctatcc aaggattgct tgaacaaatc gatgaaaaag gagaagttca aaacgtttca
    10021 atcgggacag gaatggggga cagtttagat ttttatcgga atatcggaat cacagcaatg
    10081 ccttatggac agtcgttaac ggtgctttgt ttgacagaat tgctcgtctc ttattgttaa
    10141 aagaaaggaa acccatatga aaaaaacatc actgctgttt gcagcaagtt cgcttagcct
    10201 aggtctgcta ggtctaagcg gctgcggaaa taattctagt gtggcaactg tcgcagatgc
    10261 agatgtaaat gagatagtta ccttgcgctt tgcttatgca agcaacagcc agccagtaat
    10321 tgatgcaatg aatgaatttg ggcgtttagt aaaagaaaaa acagatggtt ccgtacagat
    10381 ccagtatttc ccagatggac aactgggtgg agaacgtgaa ctgatcgaat tgacacagtc
    10441 aggagcagta gatttcacaa aagtaagtgc ctcagcgttg gaaagtttct ctaaagatta
    10501 ttcgattttt tcagttcctt atctattcaa tgatgaagaa catttcttta aggtgatgga
    10561 taatgaagaa ataatggagc caatctacca atcgaccaaa aatttaggat ttaccggatt
    10621 gacctattat gattctggtc agcgcagctt ttatatggta gacggtccga tccatacgcc
    10681 ggaagatttg gagggaaaga agatccgtgt gatgcaaagc gaaaccgcta tcaaaatggt
    10741 agagctttta ggcggttcgc ccgtacctat gggaagtgat gaagtgtaca cgtctcttca
    10801 atccaatttg atcaacggat cagaaaacaa tgaatttgtc ttgtatacag ctggacatgg
    10861 tggtgtggct aaatactatt cctatgatga gcatacacgt gtgccagata tcatcatcat
    10921 gaatgatgcc atcaaagaac agctaactga tgagcaggaa aaagccattg aagaagcagc
    10981 gaaagaatca acggtttttg aaattgaagc ttttgcaaaa gcaattgaag aagaaaaagc
    11041 tttagcacaa gaagaacatg gagtccaatt caatgatgta gataatacac cgtttcgaga
    11101 ggcagttgcc ccacttcatg atatattcaa aaacgatcct acctacaaag acctttatca
    11161 aaagatccaa gagcaaggag cgtagctgtg atgaaaatga ttttaaacaa ggtattagag
    11221 atcctgggag ctgttttgct tgttgggatg gtcgctgttg ttttatggca agtattttcc
    11281 agaacggttt taggaaatcc aaatacggtc acggaagagc ttgttcgatt cggtcttgtc
    11341 tggtttgcca tgttgtcttc cgcgtatgtt gtgggacaaa aagcacattt agccgtaacg
    11401 gttttaagtg aaaaattaac tggcaaaaaa agtaaaattt tagaagttgt tgttcaaagt
    11461 ttatttctgg tatttgctgc gatgatcatg gtgtatggcg gatggaatgc tgtcacattg
    11521 acaatggggc agatctcgcc ttctttaagt attcctatgg gatatgttta tctgtcagta
    11581 cctgtatcgg gggtactcat cattatctat agcctgatca atctaatcga tacattaagc
    11641 aacaagccag caattcaaga aaaaatataa aaatggagga ataaaatggc aatagaagcc
    11701 ggattagtgt tattttttgt atttgttgtg ttgcttgtat tagggatgcc gattgctata
    11761 agtattgcat tctcatcgat gatgacttta ttgttagtta taccgtttga tgtttctgct
    11821 tttagttcag ctcaaaaaat ggtgggaagt atcaatagtt tttcgctgat cgctattccg
    11881 tttttcgttc tctcgggcat tatcatgaat aatggaggga tagctaagaa actagtggat
    11941 tttgcgatgt tgttcgtcgg gcgcgtgccg ggggctttgg ctcatacgaa tgtgttaggg
    12001 aatgctttat ttggttccat ctctagctcg gccattgccg cttctaccgc gattggcgga
    12061 gtgttgatcc ctcagcaagt ggaagaagga tatgaccgga agtttgcgac agcggtcaat
    12121 atcgcgtcag ctccgacagg tatggtcatt cctccaagta ctgcttttat catgtattca
    12181 cttgttgctg gtggtgcttc gatctctgct ttgtttatgg gaggttatct cgtaggcgct
    12241 ttgtgggcgc taggaatcat gctagtagca ttcatcgttg ctaaaaagaa taaataccct
    12301 gttgtcgaaa aaggacagat ggctcatgct ggaaaaataa taaaagaggc gattcctagc
    12361 cttctattga ttgtcatcat tatcggtggt atcttgacgg ggatatttac agcagtagaa
    12421 gcttcagcga tagctgtcat ttactccctg ttgattgcta tgttttacta taaaacggta
    12481 aaactaaaag atatacctaa tatgttgttt caatcagtag caatgtcagg gacgatcatg
    12541 ttcttattgg ctacttcttc catgatgtct ttcgccatgg cgtttaccgg tattcctcag
    12601 gcaattagtt cgttgattat gggcgtaacg gataataaat ttattattct gttattagtc
    12661 aatcttgtgt tattactggt gggtatgttt atggatgtgg cgcctgctat cttgattttt
    12721 acaccaatat ttttaccaat cgtcacaagt gtaggggttg atcctgtaca ttacgggttg
    12781 ttttcaatca tgaatctgtg tgtaggctcc atcacgccgc cagtaggtac aggactatat
    12841 gtcggtgcaa gtgtaggagg cgtaaaagca gaggcaatgc tgaaaccgct tcttcctttt
    12901 tatggggtta tcttagtcgt cttgtttttg attacttact tcccgcaaat cgtcatgtgg
    12961 ctacccaata tgctgagtta ggagaatagt tatgttgaac attacaatta gaggacatga
    13021 tctttccgaa gtccaaacga tagaagatct tgcagaaaaa acaaaagaac aagggattca
    13081 tacactgcag ctcgccttag gtctttcgtt tcctgaaaca ccttctggcg caaatgagat
    13141 caattctggg atgggaaatt atgtcaaacg agttttagaa aagcaagaag tgaccgtagg
    13201 aattttaagt tgctatatca atatgattca tccagattta acgatacgac aagagttgct
    13261 gacaaagttc gagcagtatc ttcgctatgc ttcttccttt ggtgcgtcga tggttgcttc
    13321 ggaaactgga tgtgttttgc cggaaatcca atatacggaa gaaaatttca cagatgaagc
    13381 atttgctgaa gctgtgtccg ttatccgtcg cttggtaaaa gctggagaaa aataccaaat
    13441 gatggtgggg atagaaccag gtttgaatca tccggtgtat tctttagcaa gggtagagca
    13501 gctgatccaa gcagtagatt cggactatct aggaatcatt ttagatccga ccaatttgat
    13561 cacaagtagt gattatcaag aacaagtcca attggtagaa gaagcttttg aacgttttgg
    13621 tgaaaagata tgtgcggttc atttgaaaga ttttcgtgtc gaacaggaaa aaatcgtgcc
    13681 ggtaaatttg ggagacggtg tgatcgagta cacaaaaatc aaagaaatca tcatgaagaa
    13741 tcgtccgtac ctgtatgtgg ttcttgaaga aacaaaagat gatgggattc gttatggaag
    13801 aagtcttctt gaataatgta aagaagacgt tggttaacta atatttagaa tggagggggc
    13861 ggggctagaa gatgccacgt ctcttctttt ttataagagt atataaaagc agctcttttt
    13921 gattacaaaa aacgtggtat aataaaattg tgcataagtg aacaaaagga ggtgcggcac
    13981 atgctccaaa agaaaatcag cggtgaacaa gtcaatacga tggtttctga tcagtatgaa
    14041 atctatcatg tcaaagatgt cgtcagccaa aataaaacga tctatcatca tcatgatttc
    14101 tatgagatcc atgctacatt aaagggagaa gcgacttttt atttgaacgg acgacaattt
    14161 acgatcaaag caggaagtgt gctgctcatc cattccaatg atttgcatcg tatcgtccgc
    14221 caatcttcag atgtattcga gcgtgtctat atctttgtca ccccttcttt tctagaaagt
    14281 cgttccacgc catggtctaa tctatcggct tgttttcaac caatgggaga gaagaggagt
    14341 cgtgtattgc aaatagaaca ggaagtattg aagcaaaaat tatcatttat cgaccatcct
    14401 cctgataaaa aaatttacgg tgaagacata aaatatgaac aagctctggt agaatacttg
    14461 atttttctca atcaagttgt acaaaaagaa gagagtgcgc aagaacagaa tgctgtagtt
    14521 cctaatgagc gtgtggaaca aatgttacaa tatatctcaa aaaatttatc tcatccgctt
    14581 tctttagagg aaatggaaaa gcagtttttt gtcagcaaat accatatcac acgagaattt
    14641 aaaaagtaca ctggatttac gtttcatcag tatgtaatga agaaaaagct gatctatgcc
    14701 aagcacttgt tgcgagaata ccgcagctct agtgctgtat atagtaaaag tggtttttct
    14761 tcttatccgc attttctgaa agcattcaag agagagttcg gcataacgcc taaagaattt
    14821 ttgaaacgtg atacacagaa acagttggtt cattatgaac attttgaaga tgaacaatga
    14881 gaatgatcac taaaaaataa ccgcagtagc tgctgttgtt tacatagaag cagaaactgc
    14941 ggttatttta tttttatcta tatatttcaa aatattttga aaaaatgacg tacatattat
    15001 atgaatcagc tgaatttacg aaagtctctc ggcgatagtc ctgtttttgc tttgaacagg
    15061 cggctaaagt aagcaaaatt ctcaatccct atttcttcgg aaatcattcg aaccgtaaat
    15121 tctgtatacc gaagaaggtc tttagcgtaa gaaagttttt tgctgatcaa ataatcgatt
    15181 ggtggagaac cgataaattt ggaaaaatct ttgctcagct gatatttatt caataagaat
    15241 tgttgttcta attgatcaag tgtaatcgtt tctttgaaat gttcgtctaa gtattgcttc
    15301 agtttgtaaa cgtaggcggg aatatcctct tcttcgaaat ctagctgata cttttgcaaa
    15361 atcagttcat tcagcaactc gtggatagct acagaggact ggaaatcggt acgggcattc
    15421 gtgccttgtt gcatgtggat caattctgtg atcaaatggt ggatcttgtt ttctgatgga
    15481 tcccctgttg aatggaaggt gttatggccg tcttttgtga actcctgata aaatgattgg
    15541 acgttctgac cattcaaatg gatccaatcc atttcccatg gttcttcgct gatcgtttga
    15601 taacgctgat attcttggca gtcaataaaa aataagtctc cagcttttag ctgttgctga
    15661 ttatcattat atgtaagcga acctttgcca gacaaagtaa actttatcaa gtaagaaggt
    15721 aaatgtgctc gttcagtata gtaagggatt ggtgctttga agtaaccgat ttcttggata
    15781 taaaaaaata aattcctgct tctttgtgta ggcgtcaaga tcactctttt tgagtcttcc
    15841 gaccatgagt ggttggcttt ttcccagtaa tccatgtaaa cactcccaac ttgtcaagat
    15901 aatgcaatta tataccaaaa tagtgtgaag caaaatataa gattctgtat tatgatagca
    15961 ttgttaataa atgataacgc tttattaata aagtgctaat caacaataaa gtaaaagagg
    16021 tggagaaatg aatccaacat ttgttgccat cgatatcggt gcatcaagcg gacgactaat
    16081 gaaaagtacg agggatgcaa aaggttcttt gatcttggaa gaagtacacc ggttcaaaaa
    16141 cggctttcgg aacaaagacg gtcacgatcg ctggaagatc gattatctta tcaaagagat
    16201 tttgattgga tttgaaaaat taaagcagac agggattgat cgcctttttg cggggatcga
    16261 tacatgggca gtcgattact gtctgattga tgaacaaggg gatcggataa atgatccgat
    16321 tgcttatcgg gacaagcgta ccaatggagc agtagaacag ttttctgaaa tctattctct
    16381 agataagttg tatgagcaga cagggattca gatccagcct ttcaatacct tgttccaatt
    16441 gtttgctgaa gaaaaagaaa atttggcaaa agcacataaa ttgttactga tacctgatta
    16501 tttagggtat gtctttactg gtaacatggt gacggagaaa actaatgcat ctactatgca
    16561 gctgctgaat gcagggacaa aagaatggga aatagaactt cttgaaaaaa tcggtgtctc
    16621 gaaggaatta tttccaccat tgacagagcc gggaacgata ctaggagatc tgcaaaaaga
    16681 acaattttca aaatatgatt tgccagatgt gacctttatc acagtagcaa gtcatgatac
    16741 ggcgtcagct gtattaggaa cgccaggtca aggagaacgt tggggttata tcagcagtgg
    16801 gacatggtcg cttttaggga ttgaaacaaa gatcgccaat gtttctcgca atgctttttc
    16861 tgaaaattat acgaatgaat ggggtgccca taacacggtg cgttttttga aaaacatcat
    16921 ggggatgtgg ttgatccagg aagttgcccg aatgcaagag tacgagtaca gttatgcgga
    16981 attggcagag cttgctgcaa aagaaaaacc gctgcagcaa gtgatggaaa tcaatgaccc
    17041 tcgatttttg aatcccccaa atatgataga agagattcag caatactgtt ttgaaacaga
    17101 gcagccagtc cctgtctcat cgggagctat cgctcgttgt gtgtatgata atcttgctct
    17161 ttgttacgca caagagctga tcaaagtaga acacttaact ggaacgaaat tggaaaagct
    17221 gcacatcgta gggggcggat cgaataatcg tctactgaat caattgacag cagattatgc
    17281 cggaatcatt gtagaagcag gacctagtga agccacagca atcgggaatg ttgccatgca
    17341 aatgctcagt acaggtcagt tttcgaatat agaacaagca agaaatgtaa tcaaacagtc
    17401 gtttccatgt gaaacttttt acccagatga aaataagcaa aacacgatgg aatggtacgt
    17461 gaataaaatc aaaggagtaa tcgtatgaac acgattgaaa gaaattacga acaagcaaaa
    17521 gaaaaatatg caactatcgg tgtagataca gatgctgtac tagaaaagat acaggacatc
    17581 aaaatttcta tgcattgctg gcaaggagat gatgtcaaag gtttcttgac accggatggc
    17641 gaattgaccg gtggaatcat ggcgacaggg aattttccag gagccgcacg aacacctgaa
    17701 gagcttagac aagatctgga gaaggcttat tcgttgatac cgggcaaaca caaattgaac
    17761 cttcatgcta tctatttgga tacagaagaa tctgtcgatc taaatgaaat tgagccgaaa
    17821 cattttgaaa aatgggtaga gtgggcaaaa aaagaagaaa tcggtcttga cttcaatccg
    17881 actttctttt cgcatccaat gatgaaagac ggctttacat tggctcatcc agataaagaa
    17941 gttcgtgaat actggatcga acacgggaaa agaagccgca aaatcgcgga gtactttggg
    18001 aaagaaactg ggcaaacaag tatcaataac ttttgggtac ctgatgggat gaaagataat
    18061 ccgattgatc gttttacccc acgggaacgt ttaatggaat cactcgatga aatttttaca
    18121 gaagagttag atgaacggta tgtacaagat gcagtagaaa gcaaactatt tggtttaggc
    18181 gctgaagctt atacagtcgg ttcccatgaa ttttatatgg gttacgggtt gactcgtgac
    18241 aaacttgttt gtctagatgc tggacatttt catccaacag aagtgatttc caataaatta
    18301 tcttcattag ctttattcag caaaggaatc atgttgcatg tttcccgtcc agtgcgttgg
    18361 gattcagacc atgtggtttt gatggatgat gaattgcagg aaatcgctaa agaattagtc
    18421 cgtaacgaat tgttggaaaa aacaaatatc ggtctggatt tctttgatgc aacgatcaac
    18481 cgtatcgctg cttgggtcat cggtacgaga aatacacaaa aagcactgct gaaagctatg
    18541 ctggaaccag ttgagcgatt gaaagaaatg gagctggctt ttgactttac ttcaagaatg
    18601 gcttatacgg aagaattgaa agatttcccg tatgccgatg tatggaatta tttctgttac
    18661 aaaaatcaag ttccagttgg tttggattgg ctggaagaag tccaggaata tcaaaaagac
    18721 gtgttggaat taagaaaata ggagtgtggc tatattgaaa aaaatagata ttttacaagc
    18781 gccttatgtg aaagaaatga tcgaaacgac gtcgaatctt tatcgcttgg gatgggatga
    18841 aagaaatggc gggaatattt cttatttact aaaagaagaa gagatcacac cgttcttgga
    18901 tcctgaaaaa gtgatccgcc gcatctcgat gatttttgat gccagccgtt tagcaggatg
    18961 ctattttatt gtaactggtt caggaagata ttttaaaaat gtagcgagcg aacctgccga
    19021 gaatcttgga atcgttcgag ttgctgaaga tggcaaaaca ctggaacttt tgtgggggct
    19081 ggaaaacgaa tgccttccta caagcgagtt gccaagtcat ttcatgagtc atatcgcccg
    19141 tttagaagta gacccagata atcggatcgt tatgcattgc catgctagtc atctattagc
    19201 gatgagtttt acgcatgaat tagacgaacg ttcctttagt cgtacgctat ggcaaatgtg
    19261 tacggaatgc ttagttgttt ttccagaagg aatttccatc attccgtgga tggttccggg
    19321 aacaaatgag attggggaag caacagctga aaaaatgaaa gaaactcgat tggttttatg
    19381 gccacaacat ggaatctacg ggtcaggaaa agacatggat gaagtctttg gtttgatcga
    19441 gacagcagaa aaggcagcag aagtctatac gtacgtcaaa gcgcaaggac caatcttgca
    19501 aacaatcacg gacgaaaacc tgtggcattt agcagatgcg tttggtgtca ctccaaaagc
    19561 aggttatttg gaggaagttc atacgaaagc aggtgtctga catggtgaaa aaagcttttt
    19621 gcatgaaagt ttacccagaa aaacaagaag aatacaaaaa aagacatgat gaactttggc
    19681 cagaaatgcg tacagcactt cgtgaacacg gggtcatcga ctattcgatc tatcttgatc
    19741 cgagtacaag tttgttattt ggttacttag tgatcgaaga tgaaaagtta tgggaaaaaa
    19801 tggcaaatac atctatcaat caaaaatggt ggaagtacat ggaagatatc atggagacaa
    19861 atccagatgc ctctccagtc agttgggaat tgatgccagt ctttgaactg tagcaacaag
    19921 caactgggca accggttgct tggggttctt tcgtttttat tgtgaaaaaa aatacaaatt
    19981 aggaggaata gaaaatgagt aatcgaatga ttttaaatga aacatcgtat tttggaaaag
    20041 gagcgattga gtcgatcgtc cctgaatttc agcagcgagg attcggcaaa gcagctgtca
    20101 ttactgacaa aggattgatc gagcacggta tagcaacaaa agtaacagaa cttctagata
    20161 aagcagcaat cgcttatgct ttatacgacg ggatcgtacc taatccaacg atccaaaatg
    20221 taaaagatgg agtggctttt gttaaagagg cagaggcaga ctgtttgatc gcaatcggtg
    20281 gaggttcgcc aatcgatacg gcaaaagcca ttgggattat tttgacgaat ccagagtttt
    20341 cagacgtcat tagtttagaa ggtgttgctg atacgaaaaa tccatgttta cctatattag
    20401 ctataccaac gacttctgga acagcagcag aagtaacgat caactacgtc atcacagatg
    20461 aagtaaatca ccgaaaattt gtttgtgttg atcctcatga tatcccaatc gtcgcgttca
    20521 tcgacagcga tatgatgatg ggaatgccga aaaaattggc tgcatcaaca ggtatggatg
    20581 ctatgacgca tgctattgaa gggtttatca caaaaggcgc ttgggaaatg actgatatgc
    20641 tgcatttaaa agcaatcgaa atcatcggcc attctttgga agtttctgta gatggcgatc
    20701 aaaatgggcg agaaaaaatg gcattaggac agtatattgc gggaatgggc ttctcaaatg
    20761 tcggtttagg actggtacac gggatggctc acccgttaag tgcatggtac aacattcctc
    20821 atggcgtagc gtgtgcagca cttttgccaa ctgtcatgaa atataacaaa gaatatactg
    20881 gagaaaagta ccgtgagatc gctcttgttc tagggattaa aggagcagct gaaatgtctt
    20941 tagaagatgt aagagaagcg gcttgtggtg agatcgatcg gttgagcaaa gcggttggaa
    21001 tcccagaaac catttctgaa ttaggtgtaa aagaagcaga tattccagca attgcagaag
    21061 atgctttgcg agatgtatgc acaccaggaa atccaagaga aacgacagta gaagaaatca
    21121 ttgctttata tcaaagtttg atgtaaaaac gaagcggatc ttcaacatta aattgcctgt
    21181 aaagagaaat ggtagtttat atctggatat ctcttttaaa ttgatggtat ttccgctata
    21241 atttttctta taaagaaata ggagaatatt atatggaaaa taaaaagcct tatggaactg
    21301 tattgatcaa agcctctaaa atactagact atttagcgga aaaccaagat gtctctttgc
    21361 agacgatcgc aaagggtgtg ggaatgacct cttctacagt cttgaaaata ctagatactt
    21421 tgttgatgat cggctacgta aataaaaatt cagagaaaaa ctatcgttta ggtgcaaagc
    21481 ttattcgcta cgcgaataaa aacattgaac aaatcgattt ggtagagttg actttaccgt
    21541 ttttagaaca gctgcagcaa aagatcgatg agactatcca cttaggggta ttggacaaca
    21601 atgagatctt gtacgtgaac aaactagatc caaaacatca aacgatccgc atgtcttcaa
    21661 aaatcggaat cacccgacca ttatacaatt ctgcgatggg caaagccgta ttagcagaat
    21721 tcagtgaaga gcagtacact gattatcttg aaaagcatcc gttgatccct tacacagaat
    21781 atacgatcac caatgcatta cggttgaaaa aggaaatcca aacggtaaaa gaaacccaca
    21841 tcgcatttga cgatgaagaa atcgaacgtg atattttttg tataggagcc tcaatcgtga
    21901 aaaacgaaca aatcattggt gcatttagtg taagtatgcc gaaatatcgt ttaacagatg
    21961 agacaaaaga acaaataatc agtgccctca agcaaacaaa aagcgaaatc gagcaaagaa
    22021 ttcaaaaata atgaaaaaag cgtttgcaat ttttcttttc tgccgtataa tatgggtata
    22081 gatttccaag ttataaaata aaatttctaa ataggaaata ggtggagaag atgaaaaaaa
    22141 ttgatatttt atctcgattg aaaaacgccg gagtgattgc ggtggtacgc ggaaaaagca
    22201 aagaagaagc tttgaatgct tgccacgcaa tcatcaaagg tggtctaact ggtattgagt
    22261 tgacttttac agtccctcaa gcagatcaag taatcaaaga acttttgtca ttctacaaag
    22321 accaaccaga aatagtgatt ggcgcaggca cagtattaga cgctgtcaca gctcgcttgg
    22381 ctatcttagc tggcgcagag tatattgtta gcccttcctt tgatcaagaa acggcagaga
    22441 tgtgcaatct gtatcaaatt ccttacttac caggatgtat gactattacc gagatcaaaa
    22501 cagcgctgaa aagcggggta gatatcgtca aactatttcc tggaagcgct tatggaccaa
    22561 gtatcatttc tgccttcaag gcgccgatgc ctcaagtcaa tatcatgcca actggcggtg
    22621 tgagtctgga caatatgaaa gagtggttcg atgctggtgt cgtaacagtc ggagtaggtg
    22681 ggaacctttt agcacccgca gctacaggag attttgacaa agtaacggaa gtcgcgcaac
    22741 agtatgcagc aaaaatgaaa gaaatcaaga ggtagctgct tatgggaaaa gtagtgacac
    22801 tcggagaaat catgttgcga ttgtccacag agcagggaac ccgtatcgat tcaagtgaac
    22861 gattcgctgc ccattatggc ggaggggaag caaacgtagc aatttcttta gctaattacg
    22921 gacatgaagc gatttttgct acaaaagtac ctgataacgc attgggaaaa gcagtcaaaa
    22981 agcacttgca atgttatgga gtagatacga aacaggtatt atctggtgga tcaagactag
    23041 gtacttatta tatggaaact ggtagtggcg aacgcgccgc ctcagtgatc tatgatcgtg
    23101 ccggatcaag ttttgctgaa atggatcatt tggaatggtc gctagaggag ttattccaag
    23161 gaatagatat atttcatgtt tcaggaatca ccccagcttt gtctaaaaaa tggcaaaaaa
    23221 tgacgaaaca ggtgatagaa gctgcaaaaa aagctggctg cctgatcagc ttcgatatca
    23281 actatcgtgg aaaattatgg acacagcaag aagccagcga agtaatccat cagcttttac
    23341 ctttagtaga tatctgctca gctggaaaaa tggatgctct ttatttgtta ggtatcgaga
    23401 aagcgccaga agcagaagaa caacctctaa tttattatta tcaaaaaatg caggaaaagt
    23461 tcccgaatat tcaggtgttt tattcgacca agcgtaccgt tcattcagca agtgaaaatg
    23521 aattgaaggg gacattgtgg atgaaccatg aatatgtgga atcccaagtc catagaatca
    23581 ccccaatcgt ggatcgagta ggtggcggag atgcttttgc aggtggcgtg ctccatggga
    23641 ttttatccaa tatgtctccg caagaaatca ttgattttgc tacggcagct tctgccttga
    23701 agcatacgat ccatggggac tgcaaccaat ttagtcaaga agaagtcgca caatttttag
    23761 catgcggctc tggaaaaatc attcgataga aggagtagaa gaaaatgcaa gaaatggaaa
    23821 cacgttatac acacagtcca gaagatatcc gtcattattc aacggaacaa ttgagaaaag
    23881 aatttttagt agaaaaggta tttgaaccag ggaaaatcag tttgacttat acgcacaacg
    23941 atcgaatgat ctttggtgga gtgacaccta ctgaaaaaga attagaaatc acattagaca
    24001 aagaactagg tgttgattac ttcttagagc gtcgtgaact aggtgtgatc aatatcggtg
    24061 gaccaggatt catcgagatc gatgggcaaa aagaagaaat gaaaaaacaa gacggatact
    24121 atatcggaaa agaaacacga catgttgtat tttcttctca agatgctgaa aacccagcta
    24181 aattctatat tagttctgtt ccagctcatc ataaatatcc aaatgtaaaa atcagtatcg
    24241 accaagtaaa accaatggag actggtgaag gcctgacact gaatgaacgt aaaatctacc
    24301 aatacatcca tccaaatgta tgcgaaagct gtcaattaca aatgggttat acgattttag
    24361 aaccaggaag cgcttggaat acgatgcctt gtcatacaca tgaacgtcgt atggaagctt
    24421 atgtctactt tgattatgcc aatgaagata cacgtgtgtt ccacatgatg ggcaaaccag
    24481 atgaaacaaa acatttagtt gtagacaatg aacaagccat tatttcgcca agctggtcga
    24541 tccattcagg tgtaggtaca agcaattact cattcatctg ggcaatgtgc ggagaaaata
    24601 tcacctatac agacatggat atggtgcaaa tggatcaatt aaaataagca taagggagag
    24661 aaaatgatgg aatttagtat ggatatgttt cgtttagatg gaaaagtagc attagtcacc
    24721 ggtgctgtct atggtattgg atttgagatt gcacgctctt tagctacagc aggagcaaaa
    24781 atcgtattta ataacttgaa tcaagaatca gttgatgaag gtatagcgca ttataaagaa
    24841 gcaggcatcg atgcaaaagg ttatgtctgc gatgtgaccg atgaagaagc ggttcaagcg
    24901 atggttcaac agatcaaaga agatgtaggt tcagttgata ttctagtcaa caatgcaggg
    24961 atcatcaaac ggacaccgat gatcgaaatg gatgcggcag acttccgcca agtaatcgac
    25021 gtggatttaa atgccccatt catcgtttca aaagcagtta ttccggatat gatcgaaaaa
    25081 ggcggcggta aaatcatcaa tatctgctcc atgatgagtg aattaggacg cgaaacggtc
    25141 agtgcttatg ctgcggcaaa aggcggatta aaaatgctga cgaaaaacat cgcttctgaa
    25201 tacggtcaat acaatatcca atgtaatgga attggaccag gttatattgc tacaccgcaa
    25261 actgctccat tacgcgaaac acaagaaaac ggtgaacgtc atccattcga tcaatttatc
    25321 gttggacgta cgccagcagc acgctggggc gagccaatcg atttggctgg accatctgtc
    25381 ttcttggctt ctagcgcatc tgatttcgtt aacggccatg tcttgtatgt ggacggaggc
    25441 atacttgcct atatcggaaa acaaccttaa aagagcttta gtacactgtt tagtggagag
    25501 gttgtgaaat cagcggattg aacgaatacc gaaggacagc tgattaaaca tcatttattc
    25561 gaaaaaagag gctgggacag aagcatttaa ctccaagaaa taagaaggaa ttcacgaaaa
    25621 ttgcttttca aatttttgtg aatttcagct tatttccgaa ggagttgctt ttacttccgc
    25681 cgtttgtacg tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt
    25741 tctgtgcgtc ttccatactt cactaagtcg atccctcttg cttcgcacca atccttcact
    25801 ggtccggaaa agatcagaga ttgttggtgt agggccgcgg tattcgttgc gccgaccata
    25861 gtataaagca gacgaagctc ttcttgccat tgcttcatca gtataatagt ttcttctacg
    25921 ccgtgattca tcagatgcgt cagtacggtt cctgaagtac ccacggcatt tgcgccgaga
    25981 caaagggctt taaatatgtc ataggcattg cggatacctc cggaggctag gatttccata
    26041 gaagtgtctg cttcatttgc ttcaagcaat gaagagactg tcgattgccc ccagtcagct
    26101 aaataactca gttcccgctt ggagcgacgg gcgttttcga tctgggtgaa gcttgttccg
    26161 cttcgaccgc tgatatcaac tgtatgaact cctaaagcag ctagatcatt gagcgtttct
    26221 cttgtcatgc caaaaccgac ttcttttacg atgaggggga cgtcaatggc tgtttgagtt
    26281 tcttgaataa gtactttcca attggtaaaa tcacggtctc cctcaggcat aaccaactct
    26341 tgaggtgcat tcaaatgaat ctgtaaggca tctgcgtgaa acaatcggat agcttctttg
    26401 gctctttcga cagaagtccc tgcaccgatg ttggcaatga ttttcccgtg aggatattct
    26461 tgtcgcataa tcgtataagt atcggctaaa gaagggactt tcaaggcagc gctgactgaa
    26521 ccagtggcaa tcatcaaatc tgcttctctt gcgacgattg caagatcttg gttgattttt
    26581 tttgtttttt cgcttccgcc agtcatcgca ttgatataaa atgggctgct tagtgtaaac
    26641 ccaactgcct gtgtacttag atcgacatca gaaactgcta tttgcggcaa agggttatgg
    26701 atgatccgta cgaagtcaaa ttcgtttttt tgtttatcat gaaaggcttt agctagtgaa
    26761 acatgttcgt cttttcgatt catcggtttt cctttctttg atcgctatag acatgtaacg
    26821 gcagtggagt gatttctgct ttttcccatg cactcattaa aggaagaatg ccagatttct
    26881 ggtcaacaat tacgattccg caatcgcccc cacctgcacc agaagatttt gcggctcctt
    26941 catactgttc agctaaatta cacaatttgt tcaaagcagg cgtttcgatg acgaccccag
    27001 tgattgcaga taaatcatgc agtaattgtc ggttttttcg aatcatctgt tggatcaacg
    27061 ttacattatt ttctttaaac cctttgatca tttcgttgac acattctgta ctatttttta
    27121 agaaaagctg ataagccacc attttatctt ctctcgaacg gtgaacttga tcgaccaaat
    27181 cagaagtaga ggcagggcta cccgtccaac caatcaataa acgtaaatct tcaggagcaa
    27241 tcaatggctc aatggataga cctggccaat ccagggcaag taactcactg atagaatgct
    27301 gagtttcttg ttcttggagc caaggatggt cgaaggttga gaaagcgatc cagccaccat
    27361 aacagctagc agcgatgtcg ccgcaggaac cattatcttg aactgctaaa ttggctagtg
    27421 ctgcaatctt gaaaatctcc aactgagata aattcaacgc gtaaaaaaca ttcaaggctt
    27481 tgaccgttgc aacggtcacg gcaccgctag aacccaatcc atatttccgg ccatttgaac
    27541 tgtctaactc acttgttact ttcagatcat aaaaggataa aagaatgttt ttttcttgtg
    27601 catacttttc agtcaagcga atagcagcaa ggatataatg aaaaggattt tcccgaatat
    27661 ctaaaactaa ttctccgttg cgtcttgtcc aacgtacagg catcccacta tattgtgcag
    27721 attggatact tccgactttt cgtgcagatt ctacagtgac tgtcacgaat tgatcgactg
    27781 cagcgataac tgctggatgg cctgtttcaa caactgcata ttctccggca atatagagtt
    27841 tgcctggtgc agatacttca atcataaaaa tctcccttta ttcaataatc gcaattcctg
    27901 gaccagcatg tgcagtaatt acttgttctt tgctaaataa tttggtaaat gtttcttgta
    27961 cctcagataa atggctgttt tccactaaaa ctttgacatt agggccagca tccatcgtaa
    28021 agtagcatgg aatgccttgc tttctcaatt gtcgcaccgc atccatcgct tttaagctgt
    28081 caggagacca gtaagtgaat ggaggctggg cagctaaagt cgttccatgc atttttagtc
    28141 cgtttctttc catcgtttct ccgagaagtt ggaaatcttt tgttttgatt gcttgtttca
    28201 attgatatag atccccttca acggaatcta accagccttg ataaaaactg gatgtttcga
    28261 ctgtccgacg catcccattt ctgctggaca cttctttttt ctggtcgttg atcaaaacga
    28321 aaaccattgc taaatcgtct tcgaaagaat cggaagggac tggcttagcg taagaactta
    28381 agtcgtcatg gcctttttcc cattcgacga aaccaccgaa aatacttcgg caagctgaac
    28441 cagacccgcg tcgagcaaat cttgaaagag agagatcatc taatccaagc ttaagagcag
    28501 tattgcaagc gcctgctaaa gcagctagcc cgctggcaga ggaggctaat ccagctgcgg
    28561 taggaacgaa attctgactg attactgaag cttttagtga gaggccggct tctttacgaa
    28621 aaaggtcaag aaactgactg actttttttg ttgctttttc gtcttgcaag gtgccatcta
    28681 gataaaattc atctaccata tagctatcag aaaagatgac ttctgtctct gtataaaatg
    28741 catccaatgt taacgaaagg ctgttgttca ttggaaggat gagttcttca tttttcttac
    28801 cccaatattt gattagagca atgttggtat atgcgcgtgc tttgccttta aacataggta
    28861 tgtactccta atccttgtat ccaagtttca gcagcacccg catcttcaag tgcttgactg
    28921 atttcttggg cttttgtttt tgtttgtgct aatgcaatca tacagccacc gcgtccaccg
    28981 ccagttaatt tggcacccaa tgccccggta tcttttgctg tttggatcaa tagattaaga
    29041 aaatcattgc tgattgtcag cttttccaga agtgattgtg attcatccat tgtctgtgcc
    29101 aacgtttctg gtgaattttc aatgattgct tgttttgctt gcttcgtcaa gtatcctaat
    29161 ttttggatca tttgtccagt ttcatgaggc tgggtttcaa agaggtgagc gacatctttg
    29221 acggcttctc ttgtttgtcc tttgattcct gtatcagcga caatcaaaaa agcatcgatg
    29281 ttcaaagaaa agtagtcgaa aggatgccct tttgtaaaat aaatcggctg caagctgcta
    29341 gtagcggctg catcgattcc actaggatta ccgtgggcga ttttttccga aagctggaca
    29401 ttttctaata gtatttcacg agacaaagga aatgctaagt aatcataaaa agcacgagtg
    29461 actgctgtgg cgactgcagc gcttgatccc attccacgtt cagcaggaat cgtgctttca
    29521 atggtcagtt gcaagttttt ttggatgtca tgctgttttt ttaagtgaat gaataatttt
    29581 ttgatatttt ttaatgcatg aggagcttcg gctaaatttc ctgaatagta ggaagaaacc
    29641 agttgatcgt ccattgcatc cagctcttca aggaatgcgg tgacttttgt tgcatagaaa
    29701 ggaaaggcaa tcgctggttc tccataaaca accgcgtgct cgcccatcaa tatgatcttt
    29761 ccgcttgatt ctccttggcc atagtttgcc ataatctaac tccttctcac gtaaaagtga
    29821 tttttaagca aaaaataaag cgcccaaaac gagttgcgct gcttcacgaa tttttttgtc
    29881 cactaagggt ctcaaaaaac attcctcctt tattttacta aaaccatctc agaagtaaaa
    29941 ctaagaatac gttttctaaa gaaaaaacac ttttttctta agaatttttt gacttttttt
    30001 cttctttgac tagaaaaaaa tacaattgac actactagat attgggggta tatttaaaaa
    30061 ggttggagtt aaacacaata tttagtgatg aagtgagctg gaggtaaatg agcgtgatcg
    30121 ttttagcagg tacaattggt gccggaaaat caagtttaac agaaatgatg gctgaacact
    30181 ttgatagtca ggcattttat gaatcgattg atgataatga agtcttgccc ttattttatg
    30241 caaaccctga acagtatgct tttttgctgc aaatttattt tttgaataaa cgttttgcaa
    30301 gcatcaaaca agcaatgaaa gatgacaata atgtattgga ccgttcgatt tatgaagatt
    30361 cactgctttt ccatctaaat gcagatctag gaagagcaac agaaacagaa gttcgtgtgt
    30421 atgatgaact gctggaaaac atgatggaag aacttcccta tgctgcccat aaaaaacatc
    30481 cggatttatt agttcatatt cgtgtatctt ttgatacgat gttggagcgt atcgaaaaac
    30541 gtggtcgttc gtatgaacaa ttgtcctttg atccaagtct atacgattat tacaaagaat
    30601 tgaacagacg ttacgatcaa tggtatgaag aatacaaaga aagtccaaaa atccaaatcg
    30661 atggagaccg ctataatttt gtggaagatc ctcaagcaaa agaagaagtc ttgaaaatga
    30721 tcgaagaaaa attagcagag attcgccaaa ccaaagtagc catttgattt acagttgctc
    30781 aaataaaggg taaaccttta tttgagcgac ttttttcttt atactggaaa caaatgtcag
    30841 gaggatagag tatgcgggaa aaacactatt ttaatcaaga ccgtaatttt tggatcaatg
    30901 tacagcaaaa cgatgtgcta gctgttaaaa acttaaaaga agaatatggc attagtgacg
    30961 agatgctgac ttattctcta gataaaaatg aacgggcgcg cgtagagtac gattcttttg
    31021 atgaagcgtt actgcttgta tcaaatgttc cgcaccagca aaaagtcgat aatcattatg
    31081 aaactagtcc tatcgctttt attttaaaag aagacggttt gtttacattt accacgccga
    31141 ataccgagta cgtgattcga ctaattcgat cgcttttgga acgaatgcca gacatgagtg
    31201 tatacagttt attgtttcgt acattgtttt tgatttctga ttcctttttt cctttaatcg
    31261 aggaagtgaa tagtgaacgt cagcggctta atctgaaatt aagagaaaaa acaacgaaca
    31321 agaatctgct gcaactttca gatttagaga tcggtttggt ttacttagtt acaggaacaa
    31381 aacaaaatgt agtactttta gaacaaatca aggcattggc tatttatcgc aagttgtcag
    31441 aaaaggaaaa agaagagttg gacgatgcgc tgatcgaagc acgacaagca gttgaaatga
    31501 cgaacttagc ttcgcagatt ttagaccagc tctctggcac ttataataat ttgctgaata
    31561 acaacttgaa tgacacgatg aaatttttga cggtctggtc gctgatcttg accgttccaa
    31621 ctattgtgac aggattcttc ggaatgaatc tccagttacc ctttacccat tccgttttcg
    31681 gatggggaat cgccttgatc atcagtttgg tcttatctat ttggatgctt attgcactgt
    31741 ggcggcggat tcgttgatct agtctttgat ctgcaaacca agggcttggg cgatcacaag
    31801 tgcttgctct tggttgacga ttagtcccgc caaagaatct tgagataaag cgattgctgt
    31861 gaagaaatta gtcgtaaagt ctaatccctt cagagaagtt cgaaaccagt tcgaaccggt
    31921 caactgattg tcagacaaaa aaaggttttt ccaagtgacc tcataaaatt cgctgtcttc
    31981 taattgacaa tgatcgaagg agacagcttt caagtttgta ctactaaagg aagccatcga
    32041 agcaatacaa tcagtgaaag tacagtctcg aagataactt tcggcaaaat tcgtacctgt
    32101 taatttacat tgatgaaaca cgacttggtg gaaactgcca ccaatccact ccagattgga
    32161 aaaatcacat ttttcaaaaa tgacattact gcattcaaaa tgacgtaaaa ctgttttttg
    32221 catcgtaagt ttttggatat gactctctct taataccata tttttggcat cgagatagct
    32281 ttggtcaccc ccgttgatca gaagaccaga gaagatctcc tcatcttcta aataaaactc
    32341 tccctttgta agaagaggaa gagaaggtgg aataggttcg atttttttca tgtgggttca
    32401 cctcgttttt ctttagtata gcaaagaagc gggaatcact gctagaaagt ctgctaatct
    32461 aatcattttc agaaaaggaa gacaaactgt atgaaaaaca ttgacaaaaa gattagtttt
    32521 tgctatacta tgatggttga gaaataaaag cagaagcacc cgcttctcgc cttagttgat
    32581 aatgtcactg ggctagatag ttttcactgg ttctattagg tgaagtacgc gcgggttctg
    32641 taagagaact cgttttttta ttgccgttta ttcggtaagc ttttctttct ctcataatat
    32701 ttggaggtga atgaccatag caaaggatat gatggttaac gacggcattc gtgcacgaga
    32761 gttacgattg atcggttcag atggtgagca attaggagtt aagacaaaag cagaagcatt
    32821 gaatattgca gaacaagcaa acttggatct tgtgttagtt gcccctggtg cgaaaccacc
    32881 agttgcgcga atcatggact acggtaaata ccgtttcgaa caacaaaaga aagaccgcga
    32941 agcgcgtaaa aaacaaaaag tgatcaatgt aaaagaagtt cgcttaagtc ctacgatcga
    33001 cttgaatgac ttcaatacaa aacttcgtaa tgcacgtaag ttcttggaaa agggcgataa
    33061 agtgaaagct tctatccgtt tcaaaggccg tgccattacc cataaagaaa ttggtcaaaa
    33121 agtcttgaat cgcttagctg aagaaactgc ggatattgca acagtggaac aaaaagcgaa
    33181 aatggacgga cgcagcatgt tcttgacgct ggcaccgaaa aacgacagta agtaatttga
    33241 atgactaact ggctgttgga cagccgatag tcggataacg aaagattttg aggaggaaat
    33301 atagtcatgc caaaacaaaa aacacaccgc ggatcagcaa aacgtttcaa acgtactggt
    33361 aaaggcggat tgaaacgctt ccgcgcgttt acaagtcacc gtttccacgg caaaacaaaa
    33421 aaacaacgcc gtcaattgcg taaagcaagc atggtttcta gtggcgactt caaacgtatt
    33481 cgccaacaac tagcaaaaat gaaataagaa gtacaaataa cagaatctga agatttattt
    33541 ttattaggag gaattataca tggcacgtgt taaaggtgga acagtaactc gtaaacgtcg
    33601 taaaagagtg ctaaaattag caaaaggtta ctacggttca aaacacactt tatttaaaac
    33661 agcaaaagaa caagtaatga attcatacaa ctacgcatat cgcgatcgtc gtcaaaagaa
    33721 acgcgacttc cgtaaattgt ggattgctcg tatcaacgca gcagctcgta tgaatggctt
    33781 gagctactca aaattaatgc acggtttgaa agttgctgaa atcgacatca accgtaaaat
    33841 gttagctgac ttggctgtta acgatgcagc agcatttact gcattggctg accaagcaaa
    33901 agaagctttg gctaaataag ctaaacaatt ataaagagga aagaagataa cgaaaagtta
    33961 tgttctttcc tcttttttta tttacaatcc tccatcaaat gaaacaggac aataacgtat
    34021 aagtctaaaa tagcataatt aaggtttttt aattgaaaac tgaacattta tggtcaccct
    34081 ttaatgaaag cgctaactac ataagttaga atgagtttga ctgatatatt agtttgctta
    34141 tcagaaaaac aaaatggacg gaggaataac atgagtgata ttattattgg tattttgtta
    34201 gttatctcat tctttttcat ggtctggtac tgtgtaaaag gctacaactt gatggtcggc
    34261 tttgccatca tggctactgt atggatggga ttagcgctag tcggaaacac gttttcacca
    34321 aacccggcga tggaagggca aggagtaatt gatgtgttga cgcatatcta tacgacgggg
    34381 ccagctgagt acgccaaatc gatcttggta aatgtcttct ttggtgcctt tttcgggcgt
    34441 gttctagtag atagcggaat cgcagctacc ctgattcgaa aggtagttga acttggaggg
    34501 gacaagccaa gaatcacgat gagcttacta tgtattgtaa ccgctgttat attcatgtcg
    34561 atgacaggaa tcggaccagt gatttctatt gcagttattg tgttgccaat cttgatgtca
    34621 ttaggtattt cagtaccggt tgcactgttc tcatttatgg gctcgattat ggctggaatc
    34681 tttgctaata tcgtcaattt caaacaatat caaacgattt atgctggttt caatccagcg
    34741 gctgaaagct atacttataa tgactatttc caaattggta tgattgggat gattgtaagc
    34801 ttagttgtgg tactaacggt cgctaatata tcaatgaaca aaaagaaacg ctatgcaatg
    34861 gcagcaaacg taccagctga aggtggcgac gcaccaatga tctcatggtt agccgtatta
    34921 ttaccagttt taggggtcgt tcttttagat cttccaatca tcttaggttt tattctagca
    34981 ggtatctggg cattgctgtt tactggaaaa ttgcgcggag gttataaaga aatctgtcgt
    35041 cagtttgcga aattatttac agatggcgca gtagatgttg caccaatggt tggtttcttg
    35101 atgaccttag cgatgttcaa taattcagct gcgtatgctt ctccttattt ttcagctatt
    35161 tttggagatt ggataccgca atcaccatta gtcttggcaa tcgtatttgc tatattgaca
    35221 ccactaggat ttttccgcgg tccaatgaat ctagtcggtt caggctcagc tattttagct
    35281 gtcgtattag ctgttaatcc aacgatgtcg ccagcctttt tgttcccgtt atttgcaatc
    35341 acaacgattg ctcctcagca tttagatatt acacaatctt gggttgcttg gggattaggt
    35401 tatacaaaag taacttctcg agaatatatg aagaaatcca tcccaactgg ttggatcatc
    35461 ggagcgatct tatgcctgat cacgttctta ttgtacggga acgcctagta aacaaaacgt
    35521 cgataaaaac gattagatga atggaggaaa agaaaatgaa acgttttgtg cgaatgggaa
    35581 ttgatgttgg cggtacacat acgaaagcag tcgcaatcga taatgcgacg cacgaaatca
    35641 tcggaaaatc ttcagtgaaa acaacccacg atgatgtccg tggagtagcc gcaggtgtcg
    35701 ttcagtcttt tcaaaattgt ttgcgggaaa ataatatcag tccagaagat gttgtgtttg
    35761 ttgcacattc taccacacaa gcgactaatg cactgattga aggagacgta gcaaaagtcg
    35821 gcgtaatcgg gatggcaaaa ggtggattag aaggtttttt agcaaaacga caaactagat
    35881 tgaacgatat cgatttaggg aataagaaaa aaatagagat tgtcaatgca ttcttacctg
    35941 tcaaacactt aaatgtagac cgtgtttcag aaacgatttc ttctttagaa agagaaagag
    36001 cggaggtctt ggtttcttct atggcatttg gagtagataa tggggaacca gaacgagttg
    36061 tttatgaagc agcatcggta aaatcgatcc ctacaacgat ggcttctgac atcacaaaat
    36121 tatatggatt gacaagaaga actcggacag cagcaatcaa tgcatctatt ttaccaaaaa
    36181 tgctagatac ggcaacctct acagaagatt ccgtacgaga agcaggggtc aatgtttctt
    36241 tgatgattat gcgcggagac ggcggagtaa tggaaatcaa tgaaatgaaa aaacgacctg
    36301 ttttaaccat gctttccggt ccagcagcat cagtcatggg ctctttgatg tacttgagag
    36361 cgtctaatgg tgtttatttt gaagtaggcg gaacaacaac caatatcggg gtcattaaaa
    36421 atggacgtcc agcaatcgat tattcaattg taggtggcca cccaacatat atctcttcat
    36481 tagatgttcg ggttctaggt gtggcaggcg gttcgatggt acgggctaat cagtcaggaa
    36541 tcatcgatgt cggtccacgt tctgcacata ttgccggtct agattatgca gtatttacgg
    36601 agacagaaaa aatcaaaggt cctaaggttg aatttttctc tcctaaagaa ggagaccctg
    36661 ctgattacgt caaggtagtg atggaagacg gagaagaagt gactattaca aatacatgtg
    36721 cagccaatgt ccttggatta gtgcaagaag aacatttttc ttatggaaat gtcccatctg
    36781 cacgaaaagc catccaagca ttagctgatt attgtcatac gacggttgaa gacatcgcag
    36841 aacagatcat ggaaaaatcc tatgctaaga ttgaaccggt gattttagaa ttggcagaca
    36901 agtatcactt ggagaaagat cagatttctc ttgtaggtgt agggggcggc gctgcctcat
    36961 tgattactta ctttagtaat aaaatgggtg tgaaatattc gattcctgaa aacgccgaag
    37021 tgatttcttc tattggtgta gcacttgcaa tggtccgaga tgttgtagaa cgtatcatcc
    37081 cttcgccaag taaagaagat atacgatcgt tgaaaaatga agcgatgaat aaagcgattg
    37141 aatcaggcgc tacaccagaa tcgatcgaag tccacgtaga aatcgatcca cagacttcaa
    37201 aagtaacggc tatcgctact ggttctacgg aagtgaaagc aaccgatcta accaaggaga
    37261 tcacaacaga agaagcacta gagcttgctg cagaggatat gcgattaaat aaaaatgaag
    37321 tttgcttatt ggaaaataca cctttctttt atgtatgcgg cgaacagaat cgttcaaaaa
    37381 atgctggaag tcttcggatc atcgatcaaa aaggatttat caaggtccaa cgtggacatg
    37441 cttcatgtat gaaaacaact gcagccaatt atatgacagc tgtcgaacaa ctttgggaag
    37501 atatggcagt ttatcaaact gaattgattg cccgaccaga attttatctc tgcttaggag
    37561 caagagtctc tgattttaca gcgacggatc tggaacaact acagcttttg atggatctgg
    37621 aagtatctac tatggaaccg gaggaagaag tgatcgtcgt tgccggaaat atcaaacaaa
    37681 cataagttag aagagttact aaacgaatta caacagatcc ccgaagaaat ttggggattc
    37741 taccagtttc ggcgagattt attttggaaa aagattcctt tgtccaagca gaaaattctt
    37801 atacaacaat caatcgattg tggtatcgaa acggcatgta gcatcaaaaa aaagtaccct
    37861 tttgccgatg taggagaaat ttgtgaacaa atggctgttc caatcgtttc ctgtgaatca
    37921 gagcaaataa acgaacgaat cacttttgca acttatgcag aagatgaagg gattcgattg
    37981 atgacggagc cgttagaaaa attaaaatgt tcaggactag cttcgatctc taaagaaaca
    38041 gcgcaagctt tgattattgg acatgaacta tttcaccaca tcgaagcttc tgttaaggga
    38101 atttatacgc aaagcgaaaa aattgtgctg tggcggttgc ctttttatac acatcaatca
    38161 aacattcgtg cattgagcga gatagcagcg atgagttttt caaaagagat gaatcaaagc
    38221 gatttttcgc cttatgtatt agaggcagtc ttactttggc catataatga aattcgtagc
    38281 caagggatct tagaagaaat aaaaaaaata gaaaaaagat gtacggagta tgatttagat
    38341 tacaaataaa gagactgtga caaaagtcgt gtcacagtcc tttttttccg aataaacgat
    38401 gttctcctgt caactcctcg gtcacaagtc aaaatattca acaaacatga gaagctgttt
    38461 tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc ttggaaaacg
    38521 gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa gttaaaaaca taagaagcta
    38581 tttttacttg gctgttctta ttacttgatg cagggcagct ttttcacaac ctctcaataa
    38641 acggtgtgca atcagctgac cctcggtatt agctcaaaaa aaacggcaaa atatgaggaa
    38701 ctattttgcc gtttctttct ctaatactca ggtcaaaacg ctgattccaa aacctcttat
    38761 ttgatgggtc tgtcctgaat atcttcaaaa taatcggggt aattatgaat cacgtattct
    38821 tgctcctcga taatcatatg taaatgcatc gtgcaaagaa atccgacttc atcaaagtta
    38881 tgatcaatca ttgattgaag cattgtctga tgttcatcta atactcgacc ccagtctaat
    38941 tctgaaatcg tcaaacgtaa ccatctgaaa cggttcaagt gcgtactata actttctagc
    39001 caatcccaaa tttctcgttt tccagcaatt tcataacaag ttcggtgaaa atcagtatcc
    39061 aaatcaaaaa aagctttttt attttgagcg attactgcct gatcggtttt tccaactttt
    39121 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
    39181 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
    39241 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
    39301 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
    39361 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
    39421 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
    39481 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttgtatc ggtttgacac
    39541 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
    39601 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
    39661 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
    39721 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
    39781 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
    39841 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
    39901 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
    39961 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
    40021 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
    40081 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
    40141 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
    40201 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
    40261 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
    40321 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
    40381 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
    40441 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
    40501 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
    40561 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
    40621 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
    40681 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
    40741 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
    40801 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
    40861 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
    40921 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
    40981 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
    41041 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
    41101 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
    41161 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
    41221 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
    41281 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
    41341 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
    41401 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
    41461 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
    41521 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
    41581 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
    41641 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
    41701 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
    41761 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
    41821 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
    41881 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
    41941 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
    42001 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
    42061 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
    42121 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
    42181 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
    42241 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
    42301 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
    42361 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
    42421 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
    42481 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
    42541 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
    42601 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
    42661 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
    42721 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
    42781 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
    42841 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
    42901 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
    42961 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
    43021 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
    43081 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
    43141 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
    43201 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
    43261 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
    43321 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
    43381 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
    43441 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
    43501 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
    43561 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
    43621 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
    43681 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
    43741 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
    43801 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
    43861 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
    43921 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    43981 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    44041 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    44101 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    44161 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    44221 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    44281 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    44341 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    44401 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    44461 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    44521 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    44581 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    44641 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    44701 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    44761 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    44821 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    44881 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    44941 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    45001 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    45061 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    45121 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    45181 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    45241 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    45301 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    45361 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    45421 ttggataaat caacagtgaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    45481 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    45541 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    45601 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    45661 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    45721 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    45781 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    45841 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    45901 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    45961 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    46021 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    46081 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    46141 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    46201 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    46261 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    46321 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    46381 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    46441 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    46501 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    46561 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    46621 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    46681 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    46741 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    46801 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    46861 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    46921 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    46981 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    47041 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    47101 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    47161 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    47221 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    47281 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    47341 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    47401 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    47461 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    47521 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    47581 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    47641 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    47701 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    47761 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    47821 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    47881 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    47941 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    48001 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    48061 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    48121 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    48181 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    48241 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    48301 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    48361 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    48421 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    48481 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    48541 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    48601 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    48661 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    48721 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    48781 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    48841 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    48901 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    48961 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    49021 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    49081 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    49141 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    49201 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    49261 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    49321 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    49381 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    49441 cgccttatca taagtgatgc ggtttttatc attgatccta aaaatcttag caagacaaaa
    49501 agtacgtaag aaagtaaaga ctaaatactt cggaggaaca aatgaataac aatgataggt
    49561 tattgcgatt gcgttatgcg attgatttga aagatacgga tttgattaaa gcttttgagc
    49621 taggcggtgt accactgact aaagaagaag ctcaggcggt actgaccaaa gtacaggata
    49681 agaacaaaga taatgcagaa aataatgtgt atgaaaaaac catcaacaac caagtttttg
    49741 acgcattcct aaatggcctg attctcttgg cgcgtggtcc gcaaaaagat aagccgatag
    49801 tagacacgcc gcaaaaaagc aaggaaatca aatatatcaa caatgtgttg ctaaaaaaat
    49861 taaaaatcgc cttgtctatg accactgatg atattttaga cgtttttgct gaggcagaaa
    49921 tctatccatc aaaaggcgaa atcggggcat ttttgagaaa agaaggccaa cgaaatttta
    49981 agccttgcgg agataaatat atgcgtaatt ttctcaaggg gttaggaatt tataatcgga
    50041 gaaaagtata aagaaatttc atgagaaatc aaaacaatgg ttgttttgat gagagctagt
    50101 ttttgtatga gattaaaata ttatcttaat cgaggagctg gattggtttt atgaatgatg
    50161 aatataagaa tgatgaagat aagatgcttt ttgaagagat agagaatcga tgtaggctca
    50221 attttgagct tcggggaaag atgtctttaa tacaacaaaa aaagtattta gcgaataaat
    50281 cagagtttac tctgggacat gttgaaaaat taatctcgga ttggattagt agtagaagcg
    50341 aatttacaaa aataaaacag ccgattaaat ttgacatgaa aaaacttttg ttaaataaga
    50401 gtgagatagg taatagagat caatatatta gagctaaagg gcaagaaata attgattcat
    50461 taggtgaaat gaggtcatac aattatttgt atgtaacaca tagagctgat ggaatggtca
    50521 ttactgtggg gaaatcttct tctaatgata tttttttaga tggagatctt ttttatcaac
    50581 tcaatacaaa tcacttgtct ggcactgaaa atataatttt aaggacggaa tatggcaatg
    50641 aaatatttgc aaaatatgat gagattttaa aaaattattt ggactgggct tggataattc
    50701 cggttgagtc aggagatgct aagaaactgg agagattatt gggcgatgaa ctaattaata
    50761 aaaaagtacc aattttaaat tattattccc atagacaata aaaggctatt tttcatgata
    50821 atactagtta taactgtaaa aatggaagaa aatctgggaa agaagttttt aaataagtgt
    50881 attgttcgat tttaataaat aataagcaat accatccgtc tgtgttgcgc aacatcgtac
    50941 tacaatgaat attttttcac aaaaagaaaa tcatccatcg aatgaatgga aggttttagt
    51001 atacacgagg ttaatgcccc ctcttttata atcagaggag tgaggttttt tctatgaatg
    51061 tttctatttt cttattgcta gtctagtttc tcttacgatg aaattgggat tctattccag
    51121 tttttattga tttgctgata aaataacttg aatggtggat ataaacatga taaataagag
    51181 aagaataaca aaacaaggtt gtgaaatgat tctcaaatga atataataga aagaaaaagg
    51241 aggaagaatc aatgaaaata gcaattgcag gcgctggcgc aatgggaagt cgctttgggt
    51301 taatgcttca ccaaagtggg aacgaagtat tattaatcga tggctgggca gaacatgtcc
    51361 aacaaatcaa agagcatgga ttacaagcaa attttaatgg taaagaggta gaagcaaaac
    51421 taccaatcgt tcttcaatcc gaagtagaaa aagaagatca agttgatctg attattctat
    51481 ttaccaaagc gatgcagctg gaaaaaatgc tgcaggatat ccaatcatta atcaaaaaag
    51541 atacagaggt cttatgtcta ttaaatggta tcgggcatga agatattatt gagaaatttg
    51601 taccaatgga aaatatctat attggaaata ccatgtggac ggcaggtctt gaaggtcctg
    51661 gtcaggtcaa attatttgga agtggttcag tagaattaca aaatttaggt gatggaaaag
    51721 aagcagctgc aaaaaaatta gcagacaaat tgtctgaatc aggattaaat gctcattttt
    51781 ctgacaatat tcactattct atttatcgca aagcatgtgt gaacggaaca atgaacggac
    51841 tatgcacaat cttagacgtt aatatggctg agctaggaaa aacatcgact gctcataaaa
    51901 tggtggcgac gattgtcaat gagtttgcca aagtagcagc agtagagaag attgaactag
    51961 atgtcccaga agtcattgca cattgtgaat cttgttttga cccggaaaca attgggttac
    52021 attatccttc aatgtatcaa gacttgatta agaaccatcg attaacagaa atcgattata
    52081 tcaatggcgc aatttctaga aaagggaaga aatatggtgt tgcgacgcct tattgtgatt
    52141 tcttgactga gcttgtccat gcaaaagaag atagtttgaa cgtaaaataa aggagagatt
    52201 gaatggaaac ttcacaaaaa atatcaccga aaatttttct gaataaagtc ctcgctggta
    52261 cagcaacagg aatcattatt ggattgattc ctaatgcagt attaggtgcc attttaaaat
    52321 attttagtgc gtatcccttt gcggtaacga tcgcacagtt agcagttatt tttcaactag
    52381 caacaccttt gatcattggt ggtttgattg ccttacaatt tggatttgat ccgatgagaa
    52441 tgatggttgt agccggagct tcttttgttg gttctggtgt agtcaaattc acacctgatt
    52501 tgggtgaaac gggtgtttac gttggtgctg gtacaggcga tattattaat acgatgctga
    52561 cagcatcgat tgctgtcggg ctgattttac tgattggaaa tagttttggg tcagtgtcga
    52621 ttgtgttaac accgattgtt gtaggtgtag gtgcaggttt acttggtttt tatttattcc
    52681 cttttgtaac agcaattact tctgcaattg gatcattgat taataacttc actaccttac
    52741 aaccgctgtt aatgtctatc ttgattggtt gttcctttgc ctttttgatt atctctccta
    52801 tttcaacagt agcaatcgga atggcaatcc aactagatgg tgtttctgct ggtgcggctg
    52861 cgatgggcgt agctgcaacc acttttgttt tagtagtgaa ctcttggaag gttaataagt
    52921 caggtgtgac aattgcgatt gctttaggcg ctatgaagat gatgatgcct aatctattta
    52981 gaaaacctgt gattcttatt ccttgtttag ttagtgcagt gattacagct attcctgtcg
    53041 ctctattttc tatttcaggt acgcctgctt ctgctggttt cggcgtggtc ggacttgttg
    53101 gtccgttggc ttctttagat gcagggctaa atggctttct tgttgttctt tgttggttag
    53161 tcatccctgt ttttgcggca ctaattactc aatttttatg cgagaaagta tttaaactgt
    53221 atgatcgaga agaagtcttc aaatttttag gctagtagat tttactaaga aattttcaat
    53281 caatggatgc gtaagtgaga tgtcaacaac atctgactta cgtttttttg cctttttgag
    53341 taggagattg atgctgacaa ctgaactctt cagtgccatt cgatttttta aaatgtagaa
    53401 gaaactcacc tttatgttat tgattgttca aaaggcaact agaataaaaa tatgtgttaa
    53461 aatgagaaaa aatttgattg ttggatgtat cgaataatca ctaaaaagta ggagaagaaa
    53521 atgatttttt tgcaaaaata tggtttttat tttttattat taggtgtgat tagtgattta
    53581 tcgacaccgt atatccttgg ccttttttat cccaaactga atcaaatgac taccgtaatt
    53641 agtgtgtttg gtgacgtaga tagtccagtc agacgtgctt tcttggtctg gtcagttgta
    53701 tctggacttt tttttgttct ttcgttgcct gcgttgtatc acctatttat tggaacatca
    53761 aaaacacttg caatacttgc tgttgcaaca gttggtttat atgggatagg tgattgtatt
    53821 tttactgggt tattcagtat caatacgaat gaaagctctt ggaatctatc gacttggatc
    53881 cataatatag gttctgggct aggctatgca ggctttctac ttttcccatt gttacttgtt
    53941 ctgctttatc gtcaaagtgg tcaaggtacg cttagccatt tttatcttgt tttaacagtt
    54001 attagtttac taattgcagg tctttacgga ttagcacgga ttccgtcgat tagtcagttt
    54061 gccttcttta agcaattggg cttctttcaa cgcttaagtt ttttctttaa ctatttgccg
    54121 atgatttgtt taagtttttt tcaactacga aagtagaaaa agcagtgtga agctgattaa
    54181 gtaaaagaat ttctctatgg gtaaccattc gtgtggatgt gttcgactaa gaatagagat
    54241 gaggagagcg aaaagcaagt tagtcttaac ttgttatgcg ttgtgctaaa tttcaggtag
    54301 tcattttttg aatgaataag aagaaaactt gaaagagggt gacttcatgt tagatgtgga
    54361 gcagatggaa atagaccttt ataatattat tttaaatcga aatacaagag aatgggaaag
    54421 aacacaattt gaagaggcaa aagaagacct agcaaaaatg tcgataaagc aggtgataga
    54481 aaaactagag gtcaacttgc gtccgctagc actacgagat aatttgacac cagatgcgat
    54541 ggatttttat ttaagaatca caggggagtc agcgagtgca aaggcttatg attatgccaa
    54601 acataagata acttcaccct tataccaaga acgggcgatt tttgccggtg gctgtttctg
    54661 gtgtatggtt gagccttttg aaacaaaacc aggtgtaatc tcagtgttat ctggttatac
    54721 aggtggagag accaaaaaac caaattacga tcaagtcagt ggtggatata ctgggcatgt
    54781 tgaagctgtt gagatcatat ttgatacacg tcaaataacc tatgaagagc tgttggagat
    54841 ttattggcaa atttcagacc ctacagatgc ctttggtcaa tttcaagatc gggggaaaca
    54901 atatcgttca attctatttg ttgttaatga aaagcaaagg caattggctg aaaaatcaag
    54961 agaacaactg attcaatcta accgatttag ccgtcctatc gttacagaga ttcgccaagc
    55021 aaagactttt tggccagctg aaaatcatca tcaacaattt tatcagaagc aacctaaaag
    55081 atataaaaag atcaagcatt cgcatcagct tcatttattt tttcagcgac atagatagag
    55141 gaaggttgtg gcgcaaagcg ttcagctccg acaaaataat gagcaactga caaaaatagc
    55201 ttttcatatt tttggacagt tatgaattat ttcgaggagt tgctttgaga acaccgagga
    55261 aaaggttgtg gcgcaaagca ttcagcttca agtcgcaagg aaacaggagg tatgacaagt
    55321 cttttgtcac aactcctgtt tttttactag cctttatttc catgtgaaag agaattcgaa
    55381 ttgtagaatc ttgatttttc tatggaattt tgtagagaat catcttgata attaatagac
    55441 acctaatcga attccacatt aatgatgaac taaattccgt ataaaagcgc aaacaataac
    55501 ta
//
LOCUS       DACJGB010000011        51519 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.011, whole genome shotgun sequence.
ACCESSION   DACJGB010000011 DACJGB010000000
VERSION     DACJGB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.011"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            150..1208
                     /locus_tag="IYI34_000729"
     CDS             150..1208
                     /locus_tag="IYI34_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAR0558620.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1227..2246
                     /locus_tag="IYI34_000730"
     CDS             1227..2246
                     /locus_tag="IYI34_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAR0558621.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2348..3064)
                     /locus_tag="IYI34_000731"
     CDS             complement(2348..3064)
                     /locus_tag="IYI34_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAR0558622.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3033..3818)
                     /locus_tag="IYI34_000732"
     CDS             complement(3033..3818)
                     /locus_tag="IYI34_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAR0558623.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3809..4558)
                     /locus_tag="IYI34_000733"
     CDS             complement(3809..4558)
                     /locus_tag="IYI34_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAR0558624.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4856..5380
                     /locus_tag="IYI34_000734"
     CDS             4856..5380
                     /locus_tag="IYI34_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558625.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5396..6895
                     /locus_tag="IYI34_000735"
     CDS             5396..6895
                     /locus_tag="IYI34_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAR0558626.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6983..7585)
                     /locus_tag="IYI34_000736"
     CDS             complement(6983..7585)
                     /locus_tag="IYI34_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR0558627.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7689..8645
                     /locus_tag="IYI34_000737"
     CDS             7689..8645
                     /locus_tag="IYI34_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAR0558628.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8950..9906)
                     /locus_tag="IYI34_000738"
     CDS             complement(8950..9906)
                     /locus_tag="IYI34_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR0558629.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10080..10640
                     /locus_tag="IYI34_000739"
     CDS             10080..10640
                     /locus_tag="IYI34_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558630.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10633..11457
                     /locus_tag="IYI34_000740"
     CDS             10633..11457
                     /locus_tag="IYI34_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAR0558631.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11467..12084
                     /locus_tag="IYI34_000741"
     CDS             11467..12084
                     /locus_tag="IYI34_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAR0558632.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12102..12785
                     /locus_tag="IYI34_000742"
     CDS             12102..12785
                     /locus_tag="IYI34_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAR0558633.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13468..14751
                     /locus_tag="IYI34_000743"
     CDS             13468..14751
                     /locus_tag="IYI34_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0558634.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14767..15654
                     /locus_tag="IYI34_000744"
     CDS             14767..15654
                     /locus_tag="IYI34_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0558635.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15671..16507
                     /locus_tag="IYI34_000745"
     CDS             15671..16507
                     /locus_tag="IYI34_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0558636.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16510..17133
                     /locus_tag="IYI34_000746"
     CDS             16510..17133
                     /locus_tag="IYI34_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558637.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17130..18422
                     /locus_tag="IYI34_000747"
     CDS             17130..18422
                     /locus_tag="IYI34_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR0558638.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18781..18878
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18994..19575
                     /locus_tag="IYI34_000748"
     CDS             18994..19575
                     /locus_tag="IYI34_000748"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAR0558639.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19578..20885
                     /locus_tag="IYI34_000749"
     CDS             19578..20885
                     /locus_tag="IYI34_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAR0558640.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21301..21789
                     /gene="purE"
                     /locus_tag="IYI34_000750"
     CDS             21301..21789
                     /gene="purE"
                     /locus_tag="IYI34_000750"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAR0558641.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21773..22906
                     /gene="purK"
                     /locus_tag="IYI34_000751"
     CDS             21773..22906
                     /gene="purK"
                     /locus_tag="IYI34_000751"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAR0558642.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22919..24214
                     /gene="purB"
                     /locus_tag="IYI34_000752"
     CDS             22919..24214
                     /gene="purB"
                     /locus_tag="IYI34_000752"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAR0558643.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="IYI34_000753"
     CDS             complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="IYI34_000753"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR0558644.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26293..26670)
                     /locus_tag="IYI34_000754"
     CDS             complement(26293..26670)
                     /locus_tag="IYI34_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558645.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26926..27654
                     /locus_tag="IYI34_000755"
     CDS             26926..27654
                     /locus_tag="IYI34_000755"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAR0558646.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27654..27908
                     /gene="purS"
                     /locus_tag="IYI34_000756"
     CDS             27654..27908
                     /gene="purS"
                     /locus_tag="IYI34_000756"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAR0558647.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27910..28581
                     /gene="purQ"
                     /locus_tag="IYI34_000757"
     CDS             27910..28581
                     /gene="purQ"
                     /locus_tag="IYI34_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAR0558648.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28582..30804
                     /gene="purL"
                     /locus_tag="IYI34_000758"
     CDS             28582..30804
                     /gene="purL"
                     /locus_tag="IYI34_000758"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAR0558649.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30789..32228
                     /locus_tag="IYI34_000759"
     CDS             30789..32228
                     /locus_tag="IYI34_000759"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAR0558650.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32260..33303
                     /locus_tag="IYI34_000760"
     CDS             32260..33303
                     /locus_tag="IYI34_000760"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAR0558651.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            33300..33878
                     /gene="purN"
                     /locus_tag="IYI34_000761"
     CDS             33300..33878
                     /gene="purN"
                     /locus_tag="IYI34_000761"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAR0558652.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33875..35410
                     /gene="purH"
                     /locus_tag="IYI34_000762"
     CDS             33875..35410
                     /gene="purH"
                     /locus_tag="IYI34_000762"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAR0558653.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35426..36700
                     /gene="purD"
                     /locus_tag="IYI34_000763"
     CDS             35426..36700
                     /gene="purD"
                     /locus_tag="IYI34_000763"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAR0558654.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36822..37736
                     /locus_tag="IYI34_000764"
     CDS             36822..37736
                     /locus_tag="IYI34_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAR0558655.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37869..39434)
                     /locus_tag="IYI34_000765"
     CDS             complement(37869..39434)
                     /locus_tag="IYI34_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAR0558656.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39427..40878)
                     /locus_tag="IYI34_000766"
     CDS             complement(39427..40878)
                     /locus_tag="IYI34_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR0558657.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40892..41908)
                     /locus_tag="IYI34_000767"
     CDS             complement(40892..41908)
                     /locus_tag="IYI34_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0558658.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42128..43216
                     /locus_tag="IYI34_000768"
     CDS             42128..43216
                     /locus_tag="IYI34_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAR0558659.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43270..44796
                     /locus_tag="IYI34_000769"
     CDS             43270..44796
                     /locus_tag="IYI34_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAR0558660.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44798..46003
                     /locus_tag="IYI34_000770"
     CDS             44798..46003
                     /locus_tag="IYI34_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0558661.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46260..47237
                     /gene="guaC"
                     /locus_tag="IYI34_000771"
     CDS             46260..47237
                     /gene="guaC"
                     /locus_tag="IYI34_000771"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAR0558662.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47501..48925
                     /locus_tag="IYI34_000772"
     CDS             47501..48925
                     /locus_tag="IYI34_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAR0558663.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            49386..50495
                     /gene="pdhA"
                     /locus_tag="IYI34_000773"
     CDS             49386..50495
                     /gene="pdhA"
                     /locus_tag="IYI34_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAR0558664.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50498..51475
                     /locus_tag="IYI34_000774"
     CDS             50498..51475
                     /locus_tag="IYI34_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAR0558665.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        16256 a         8998 c        11273 g        14992 t
ORIGIN      
        1 aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat aagtaacaat
       61 gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt ttttattgtg
      121 aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt cgatcgactt
      181 tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata ttattagggt
      241 ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta tcgaaagtcg
      301 tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat atcaatacag
      361 aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat attattctta
      421 ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca acgttgcgtg
      481 tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt gcgatttatg
      541 gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc tttttgtatt
      601 cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc aaagcgatgt
      661 tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg ttccaagggt
      721 atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta gcgtttttgc
      781 gaattgacca gactgcaccg tggctatttc agccgatgga ttatttagct ggtttaagtt
      841 caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc aaaaaagctg
      901 catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta cctgcattga
      961 atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat tttgaagcac
     1021 tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca gcctatgcta
     1081 tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca actatttttg
     1141 ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc ataggtttat
     1201 tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt cgtccgaacg
     1261 agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt tatgaagaac
     1321 tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg ttgaggggaa
     1381 attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt cgttatgttt
     1441 ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat tttggtatga
     1501 cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct ttgacttttg
     1561 ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc aaagattttc
     1621 gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg atcataggaa
     1681 caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc aaagtgatcg
     1741 gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac tcgcttgaag
     1801 agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca ggtgaaaacg
     1861 atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc ttgattaata
     1921 cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa agcaatcatt
     1981 tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc cgatccttca
     2041 agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg taccctagag
     2101 ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat atgatttcga
     2161 caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat gaggtatctc
     2221 ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa aatgtttaaa
     2281 aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta tgggataatt
     2341 cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat tcataacagg
     2401 ttagcggccg gccgatttcc aaatatttga ttctttcgtt caatatatct tgttctacta
     2461 aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga gccagttctt
     2521 ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt aaagtcagtt
     2581 ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga gtggtgtggt
     2641 tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt gtctgagaga
     2701 aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt ttgatcaaat
     2761 aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct gcagcagtga
     2821 tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa tggataccac
     2881 tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt ctgaaatatt
     2941 tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg atgctttcca
     3001 caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg acatttgtca
     3061 tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc atttgtttgt
     3121 ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag attcaatcca
     3181 aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc tgagatttct
     3241 tctgatatcc cgacaccgtc atcgcagacg gtcacgatga atacttcagc ttctgtgtca
     3301 tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac ggcttctttt
     3361 gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc atccatctcc
     3421 attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc tttggcttcg
     3481 tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact tgagacttgg
     3541 ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac ttcttcatac
     3601 ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc atgggtttga
     3661 gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa tttcattact
     3721 tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct aaaaagaggt
     3781 gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg aacggtcgtt
     3841 tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat ttttttccta
     3901 cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt gtattgatca
     3961 agaggatagt tttatcagaa gagatggcta agattccttc atctacttca ttttcgataa
     4021 tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc agtacttttt
     4081 tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca ataatcaatg
     4141 aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg tttgttaagc
     4201 caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa tagcggaagc
     4261 cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc acttttttca
     4321 catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga gaatatcgaa
     4381 tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg atttcatttg
     4441 ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg atgagtttat
     4501 cattggcaac gattttagca atgctagaga ttttatctga cacactagat cggttcatcg
     4561 aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat gtggagaaac
     4621 aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa aaaggaaaca
     4681 taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa atttaacaaa
     4741 ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta aaagaagcta
     4801 tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg tgtatatgat
     4861 agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg caggattggc
     4921 agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg atccgatgct
     4981 tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc gtttaacaga
     5041 tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt tggtattttt
     5101 gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt cgtcttctga
     5161 aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac gaggtatggg
     5221 agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt tttacagccc
     5281 ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca aggaaagaat
     5341 ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt gttaaatgga
     5401 aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga tgttgctgat
     5461 cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca tcagtggaag
     5521 ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca aaattgtgcg
     5581 tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca gtctattatc
     5641 gagtaaattg gacgtctcca cccctgaagt gtatacggaa caaggcgtac cggtgatgtg
     5701 tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta cagcagcaga
     5761 acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag tattagaagg
     5821 acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa atcgcgataa
     5881 gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg ggttgatcat
     5941 cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact ctttagggaa
     6001 accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag ctctaaaaga
     6061 aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat tacagcgaca
     6121 aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct acaaacaaga
     6181 acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg cccgagcaca
     6241 acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgtcaaa aacaaatcga
     6301 actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag tgaagaaaaa
     6361 agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag tcaaagaagt
     6421 gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg aagccaataa
     6481 gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa aagcagaagc
     6541 cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag ctgtcttgag
     6601 tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc taggaaatat
     6661 cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg gagcgaaccg
     6721 agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa aagcgacaac
     6781 aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta ctagccaagt
     6841 aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag aataaagtag
     6901 tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc tctccatttt
     6961 tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt tggatcttag
     7021 cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt tcgaccaaat
     7081 cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa ttgataccga
     7141 ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac aaagcggatt
     7201 cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa atgataccgg
     7261 aattattgtt aataccatct gacacgacga tcacaataat aactagaata ggaagcaaga
     7321 tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa atgatagcta
     7381 atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg gttgtagtca
     7441 agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt cctcgttgag
     7501 tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc gtacgcatac
     7561 ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt actatcatac
     7621 ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa taaaaaaggg
     7681 ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc ttcttttcga
     7741 tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc attgcattat
     7801 taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga atcgcagcct
     7861 ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat actatttttg
     7921 gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa ttaaccgaag
     7981 ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac atgtcttggc
     8041 agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct ttgcttgcaa
     8101 atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag atcgaaaaag
     8161 aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat aaagaagatg
     8221 tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa gatgccagag
     8281 aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac ttctctatga
     8341 gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga atccagcaac
     8401 cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa acattttcag
     8461 agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta ttgtatcggc
     8521 aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg ttccagtttt
     8581 tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa aaaatccaaa
     8641 aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac aacctcttct
     8701 ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa caaacatgag
     8761 aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct cacaacctct
     8821 ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa acgaaaaata
     8881 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
     8941 cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt cgatgacatc
     9001 ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta ccatattctt
     9061 tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct ttgtaccatc
     9121 tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca cataaccgtt
     9181 ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc tgacagcagc
     9241 aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc ctgtttcaaa
     9301 aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac gagcgaaaga
     9361 tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat tgaagaaccg
     9421 gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt ttaaagcccc
     9481 cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt tgaagattgt
     9541 catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt cagccagttc
     9601 ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt ccaatgctgc
     9661 caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt cgatttcagc
     9721 atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac ggtgagcgaa
     9781 gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg tctcttgatt
     9841 gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc attgatattt
     9901 tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct attattataa
     9961 caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat gaaagcagcg
    10021 atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag gggataaacg
    10081 tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt gaaactgaaa
    10141 aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa tggaaagatg
    10201 gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc actgattatg
    10261 aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa aaacgaaacg
    10321 ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg cttagcagca
    10381 aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt agataccttg
    10441 acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg aattttcgtc
    10501 tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc gattgggtga
    10561 atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa gaacaacgaa
    10621 aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat gatcgcagca
    10681 atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa tcaagcagtc
    10741 gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac aaccagcagc
    10801 aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga tgttaaaaca
    10861 gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga aattgatgag
    10921 tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat tgctccagaa
    10981 tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat ggtctcagct
    11041 taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga tgtgatgagt
    11101 cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat tacggagcca
    11161 ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa ctggtacaac
    11221 agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc taaatggatc
    11281 gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca cgaagatata
    11341 actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa cgctgagtat
    11401 atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga aaagtaggaa
    11461 ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg ttggccagca
    11521 gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa gaatctgtct
    11581 tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca atttattgaa
    11641 cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc tagtatcatt
    11701 ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag caaatacaat
    11761 aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga aacaaaggaa
    11821 tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga ttcatgggaa
    11881 cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa tcctgcgctg
    11941 tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt gcaagatgca
    12001 ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga gacgtatcaa
    12061 ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa caatttacgc
    12121 cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc attttagaag
    12181 caagcgggat caagatattt ggaaaattga tccgttcgat catctttact acggatattg
    12241 cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt acacctcatc
    12301 cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc ggtgtaggtg
    12361 gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa gcgcaaggtt
    12421 cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa gtttgtcgag
    12481 tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata gatgaaaagt
    12541 tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca gaagcagtac
    12601 gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt ccaacggaag
    12661 aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg ccaccaagta
    12721 gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa aagaatcatg
    12781 aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc tcgtcttttg
    12841 tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt ttttttacct
    12901 gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa caaaaaaatg
    12961 tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta aaaaaggcac
    13021 ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga actgaccaat
    13081 caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat ttcatacaga
    13141 attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca tgcttattta
    13201 gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt aattatcctc
    13261 ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact atctaatttt
    13321 tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct tggtttttat
    13381 tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt atattgaaaa
    13441 ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat cttagcttta
    13501 agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa aatcacgctg
    13561 aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt aatcgagctt
    13621 tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta tgaaggatat
    13681 tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt ccaactgaac
    13741 aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt ggagcaaggg
    13801 ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta cgacgggcaa
    13861 ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa aaatatattt
    13921 gaacaattaa atatccctga accagcttca aattggacat gggatgaatt tgaacaaatc
    13981 agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga agattttata
    14041 gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta taatcgggaa
    14101 gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt tttccagctg
    14161 cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc tgcaagtacg
    14221 acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt tctatcaagt
    14281 aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca gttgattaat
    14341 ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc gcaaatgctg
    14401 agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga tttctttcaa
    14461 aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat catgtcgaat
    14521 attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt tgattattta
    14581 gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc tttgaatccg
    14641 gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga agtaacagca
    14701 gaagaagcag cagagagtct ttatgagttt gcgtcaaaca ttctaggata gcagcaggag
    14761 gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt ggtcggttac
    14821 gtatttgctt ctccattcat cctaggattt ctattgataa cagtgatacc gatgatcttg
    14881 tcactttatt attcgttcac cgattatcgt ttgggaaatc cagtctcatg gatcggcttg
    14941 gataattata taaagatgct gagtgatgcc cgttttttga actccatccg agtcactttt
    15001 ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg gtctattgat tgcttatctt
    15061 ttaacgagga atatcaaagg aacgactttc tttcgttctt tgtattatgt gcctagtttg
    15121 atcggtggca gtatttctat cgctctagtt tggcgggagt tgttttctaa aaacgggtta
    15181 gtgaatattt tcctggaacg attaggcgca cctgcgcttt cctggtttag cgatcaaaaa
    15241 ctggtcatga tccctcttgt tttgatgtct gcttggcaat tcggttcggc aatgatcatc
    15301 tttgctgcag gtatcaaaga gatcccaggc tcgtattatg aagcggcaga gatcgatgga
    15361 gcgaacaaat ggcagacgtt ttacaagatc acgttacctc ttctttcgcc gatcattttg
    15421 tataacttgg ttatgcagac aatcgcggcg tttatgactt ttacgcaggc atttatcatt
    15481 acccaaggtg gtccgaatga tgccacgaac ttttatgctc tttatgtgta taaccaagct
    15541 ttttctttta attctatggg gtatgctagt gctatttcgt ggatcatgct gttgattatg
    15601 ggagctgtca cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt gtgaatgaga
    15661 ggaggaagaa atgaataata cagtaacgaa atggtttcaa tacggtttta ttttgctatt
    15721 aggtctagtc atgatttatc cggtcttatg gatgatcgca ggttctttta aaacggatca
    15781 agagattatg agtggttcgc tgaatttgat tccagagact tttcaatggg aaaattacgc
    15841 aaaaggatgg gcggggtttg ctggaatttc attttttact ttctttagga attcgtttgt
    15901 catctctgca gtttcgacga tagggacagt tctgagctcg acttgtattg cttacgcatt
    15961 atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag taacgatgat
    16021 gattcctgca caagtgattt tgattccgca gtttattatt tacaatcggc tgaatttggt
    16081 tgggacgtat gtgccgctga ttttaccaca tttttttgga caagcatttt tcatttacca
    16141 gatcatgcaa ttcatggtca gcatccccaa agagttagat gaatcagcga ttattgacgg
    16201 atgcagcaag tatagtgtct ttaccaaaat tatttttcct ttgctgaaac cttcgatcat
    16261 cacgacgatc atcatccagt tttattggaa atgggacgac ttcatgggac cattgattta
    16321 tttaaacaag ccgagatctt acacggtatc gattgccatc aaattatttg cagacgctgg
    16381 atctagtacg acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt
    16441 tctgattttc ttattcttca ataaatatct tgtccagggg atcagtacaa gcggtctgaa
    16501 gggctgaata tgaaaaagcc aatagaagag atgtttcttt acaagctcaa ctactatgtc
    16561 tgtcggattt ttttaaccag tcttatcagt aatgctcttt tcgctttagt tctaggatgt
    16621 ttgttcactt taggattagt gctgtctgct cgtgttgtct atctgattta ttttttgatg
    16681 atgtatcctt cttattatct atctgtccat tacttggcag aaaaaaaggt atgttggagg
    16741 aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt ttcagctgtt tgtcattatt
    16801 tttgcagttg attggtattt attagtggga aagattggag cttggtatct gattcctttt
    16861 ctattgtgtc tgttgctttt tgtatcccat agttttttta gtacgctaat tgatatctgc
    16921 aaagaagata aagcagatag tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca
    16981 aaaaacgtca aggggtttct gtggtattta tgccttttgt atggactgtt ctggtgtata
    17041 cagcatgatt ttttactcac tgcattactg tatccagggc tgccatttgt cacagtattt
    17101 tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg
    17161 gtgggagagc gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac
    17221 tagtcggttt ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg atacttgtga
    17281 acaagtatgg acagaaaaaa gtaaagacat gtatgccatc acaatttgag caactactgg
    17341 atgacgtacg gccggctgaa gtcattatta cgtcagtaga tcgaacgcat catgattata
    17401 tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg actatcgatg
    17461 aggaaaaagc tcaagaaata ataacggtcc aaaaacagac gggaagaagc ctgcgagtca
    17521 cttttaatta tcgttatgca ccgtatcaca cgaagataaa ggaactgata gaaagtgatg
    17581 tgattggtga tgtgttttct atccacttcg aatggctgtt aaatacgcaa cacggggcag
    17641 attatttcag aagatggcat cgaaataagc acaatagcgg cgggcttctt gttcataaat
    17701 ctacccatca ttttgatcta gtgaattttt ggttgaacag ccgtccccaa aaggtatttg
    17761 cattcggtgg attaaacttt tatgggatgc ataatgcgga gaaacgcgga gtagaaatat
    17821 tttacagtcg atgctatgga agcgaactag ctaaagctga tccatttgct atagacctgt
    17881 cagccaatga ggaattgcga gaaatgtatt tagaagcaga aaaagacagt ggatatattc
    17941 gtgaccgcag tgtattttcc gatgatatct ctattgaaga tacactaggc gtaacggtca
    18001 aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat
    18061 tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt
    18121 acatcaatgc aggtggagat aaaaaggatg aaggagcagc cgttcttcag tccatccatg
    18181 tctatccgat gttcggtgag ccatatgagg taatcgtcga acaagaagcg ggcggacatg
    18241 gcggaggaga tccgcgttta ttagatgatc tatttggtga agaaaaagaa gaagatccgt
    18301 ggaatcgggc agcaactcac gtagatggta ttttgtcgat tttgacagga atcgcagcaa
    18361 atcattctat tgcttcagga aaagcagtag tgattgacga gcttgtcagc ttaaaagaat
    18421 aagaatagat ttacggaaat tacttcccgt agtttataaa atcttgcctt attgccgata
    18481 ggctgtcttt tgaacactat ttattcggaa aaaggggcta tgacatgctt tttgtcatgc
    18541 cttcttttta tgtgggctgc ttttttagat aggagaaaat aaaaataact atttaaaccc
    18601 gaacaaaaag gaataatgca ttaaaaatca ttagatttta caaaaaaata agtaaaccac
    18661 gttgtcagtt tcagaaagta gtgttagact gttttcaata tttgaaacaa cattttttat
    18721 tcaaatatgg caaactgcct tcacgtgcaa aacagttgca agaaaatgaa tttacactat
    18781 tattagtttt acatacctac ttatatatcg tcataatatg gatgacagtt tctagccagt
    18841 accgtaaatg ctggactata agtaaaagat tggctatttc acccttcttt tcttatgata
    18901 aagtggaggg tgtttttctt taacagaaac atagtaggtt tttgcacaaa agcggttatt
    18961 tcggtattaa taaatgataa aggagcaaat aaagtgaaag aattagttga acgtattcaa
    19021 aaagacggac gtatattagg cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa
    19081 gtagaccctg tattgatgga acaaatggga aaacggtttg cagaagtatt taaagaacaa
    19141 ggaatcacga aagttgtgac cattgaagct tctgggatcg caccagcttt atttgctgca
    19201 agagaattag atgttccaat gatcttcgct agaaaagcaa aaagtttgac gatggatgaa
    19261 gaattattga cagcttctgt ttattctttt acgaaacaag tgacaagtac gatttctatt
    19321 tctcgtaaat ttttatcttc agaagacaaa gtattgatta tcgacgattt cttggcaaac
    19381 ggacaagcgg caaaaggatt gatcgaatta tgccagcaag ctggagcgca agtagaagga
    19441 atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc agttacttga agaaatggga
    19501 ttgagagtcg tttctcttgc gcgtattgct tccttgacga atggacaagt tgagtttctt
    19561 gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc
    19621 tgcagcatct gctagcgatg tatgcaggag cagtagcggt gcctcttttg atcggtacag
    19681 gactaggctt taatcaagaa cagatgactt atttgatttc aattgatatc tttatgtgtg
    19741 gattagcgac attattgcaa ttgacagtta accgattttt cggaattggc ttgcctgtcg
    19801 ttttaggatg tgcgatccaa gcagtagctc cactgatctt gatcggaacc aatgaaggag
    19861 taggagctat ctacggatct attatcgctt ctgggatctt tgttgtgtta gtttctggtg
    19921 tcttttctaa aattaaaaaa ctattccctc ctatagtaac aggaacggtt atcaccgtta
    19981 ttggtttgac tttgattcct gtagcaatcg agaaaatggg tggaggtaat gcaacagcag
    20041 ctgattttgg tgacaaaaca aatctgctac ttgcatttgt gacgattctc ctaattgtag
    20101 gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc ggtattgatt ggtttagtcg
    20161 gaggaagcat cttagctgct ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc
    20221 cgttgttcca tgttccacaa cccttttatt ttggtaaacc aactttcgac gtatggtcga
    20281 ttttactaat gatcatcatt tccatggtca gcatggtaga atcaacaggt gtatattttg
    20341 cacttggtga cattactggg aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg
    20401 cagaaggact tgccgttatc ttaggcggta ttttcaatac attcccttat actggctttt
    20461 ctcaaaatgt cggattggtt caactttcgg gaatcaaaac aagaaaaccc atctatttct
    20521 ctgcattctt cttgattgtt ttaggattat tccctaaaat cggtgcgtta gcacaaatca
    20581 tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag
    20641 gaatccgtat gctgttggaa gttgatttca gcaatgataa aaatctattg attgttgctg
    20701 tttctgttgg atttgggctg ggtttcaatg ttacgccgac attattcagt caaatgccgc
    20761 aaacattgca aatgttcact ggaaatggca tcgtaatgag tagcattacg gctatcatat
    20821 tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat attggaagaa gccttcaaga
    20881 agtaatagag caaaagaaag gttgtgaaat cagcgttttg acctgattgt tagagaaaca
    20941 aacggaaaaa tagttcctcg tattttgccg tttgtttgag ctaataccga gggtcagctg
    21001 attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa
    21061 caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt tccttgtgac cgaggagttg
    21121 gcaggagaac accgtttatc cataaaaagg aatgtgacct aacttttgtc acattccttt
    21181 tttttatctt taataaacac gaacaataaa atgtttttct ttgttaacat tcgaaatttc
    21241 tttgacatca aaaaaagtga ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca
    21301 atgacagcaa aaatctctgt gattatgggg agtacttcag attgggaaac aatgaaagaa
    21361 gcatgtcaaa tattggatga attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat
    21421 cgaaccccag atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc
    21481 attattgcag gagcaggggg agccgcgcat ctgccgggta tggtcgcatc aaaaacaact
    21541 ttgccggtta tcggtgtacc ggtccaatca cgaacattaa atggtttgga ttcgcttctt
    21601 tcgattgtcc aaatgcctgg aggagtacct gtagctacta cagcaatcgg aaaagcggga
    21661 gcaattaatg ctggactact tgcgatacag atgctttcca tgtatgattt agaattggag
    21721 gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa
    21781 cttggttaaa cctttgttgc ctggttcaac gatcgggatc gtcggtggcg gtcaattagg
    21841 tcgtatgatg gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt
    21901 gagcgattgt ccagccgcac aagttgcaga ttggcacatt gaaagtgtct atgatgacac
    21961 ctttgcgctg aaagagcttg ccagaagaac agatgtcatt acctatgaat tcgaaaacgt
    22021 cagtgtagag gcgttaaatg cgattttacc catgtcattt attcctcaag ggacagactt
    22081 gcttgcgatt acgcaagacc gcctgttaga aaaatctttt ttggaaacga ataacatcgt
    22141 gattgctcct tatgcaacga tcgtcagtcc gacggatatc caagatgcga ttgacgggat
    22201 cggctatcct tgtgttttga aaaccactcg aggcggctat gatggcaagg ggcagtatgt
    22261 tttgaaaagt cgggcggatc tagctcctgc catggacctt ttaagagaag gaacctgtgt
    22321 tttagaagca tggatcccgt ttgaaaaaga gatttcgatc atggtggcag gaaatggtca
    22381 aggagatttc acgacttttc cggtcgtgga aaacatccac cacaacaata ttcttcatga
    22441 aacgattgct ccagccgcga ttgatcagga tgtgatcgag gaggctgagc gtattgcccg
    22501 tgtgattgca gaagcagtga gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa
    22561 aacaggcggg ctatatgtga atgaattagc tcctcgtcct cacaattctg gacattattc
    22621 tattgaagct tgttctatga gtcagttcga tgcccatatt cgaggtattt gtggttggcc
    22681 attaggagaa aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca
    22741 actgctggaa agctatcgct tgattgagaa taagccagac tggcaattcc atttctatgg
    22801 aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc actatcttga cagaagacat
    22861 ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg gattagagga gaaaaaacat
    22921 gttagaacgt tatacacgcc ctgaaatggg gaatatctgg acggaagaga acaaataccg
    22981 tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc
    23041 gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc ggatcttgga
    23101 aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt
    23161 gggagaagaa agaaaatggg tccattatgg tctaacaagt acggatgtcg tagatactgc
    23221 ttatggctat ctcatcaagc aagcaaatga tattttacgg gaagacttga aacgttttac
    23281 ggaaatcatt ggcgaaaaag caaaagaaca caaatataca gtgatgatgg gtcggacaca
    23341 cggtgtccat gctgaaccaa ctacttttgg attgaaactt gccacatggt attcagaaat
    23401 gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc
    23461 aggagcagtg ggaacgttcg caaatatccc gccatttatt gaagagtatg tatgtaaaca
    23521 attggggatc cgtccacaag aaatctctac tcaagttctc cctcgggatc tacatgctga
    23581 atatttctca gcgatggcgc ttattgctac aagtattgaa cgttttgcaa cagagatacg
    23641 cggattgcaa aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg
    23701 ttcttctgct atgccgcaca aaaggaatcc gatcggttca gaaaatatgg ctggtctagc
    23761 cagagtggtg cgcggacaca tggtcactgc ctatgaaaat gtcacattat ggcatgaaag
    23821 agatatttct cattcttcag cagaacgaat catcttgcct gataccacga ttttattgaa
    23881 ttacatgtta aatcgttttg gcaatatcgt taaaaatcta acggtctttc ctgaaaacat
    23941 gaagcggaat atgtctgcaa catttggttt gatttatagc cagcgcgtta tgctgaaatt
    24001 gatcgataag ggaatgacaa gagaagaagc ttatgatctt gttcagccaa aaactgcgta
    24061 cgcttgggac aaccaagtgc agttccgccc gctgttagaa gaagatccaa ccatcacatc
    24121 ggtattatct aaagaagaaa tagacgatgc ctttgattat catcatcatt tgaaaaacgt
    24181 tgacgtgata ttcaaacgag taggtttgga ataaagaaga gtgagttgca aaattcaatt
    24241 aaaatagaat agcaaaaata atcgagtaga ggggaaagcc aactgacctt ccgcccctct
    24301 cacaccaccg tacgtacgga cccgtatacg gcggttcaat aagttgagtg tacgaactgg
    24361 tacttcaagg agatgtcctc atatccttgg cgggccagtt ctttgtctgt taatgatcga
    24421 tgaagaatat gacttttgga gactgaccaa ataccttttc ttgtatttga ccattcatac
    24481 gctttcgctt tatcgatccc taaacgaatc agattttttc ttttggttct tggattcttc
    24541 cattgtttcc aaatatattg tctgattctt cgcttcaacc aaccgtttaa ctccctcatg
    24601 aattctttca tctcaccaat tccatagtag ttgatccaac cagtcattag ttgcttgatt
    24661 tcttttaaaa taagttggat acttcttcca cggtttcttt tagtgagttg tttgagcttg
    24721 cttttcacac gctgttttgc ttttgcgtga gggcgaattt taataccttt ctttgttggc
    24781 agaatgcaga atccaagaaa ctttcgtttc aatggacttc ctaccgcact cttttctcga
    24841 tttaccgtca actttagttg ttcttcgaga aattgaatgg atttcttcat cacacgctgt
    24901 cctgctcgtt tgcttctaac atagatattg cagtcatcgg cgtatcgaac aaatttatgt
    24961 cctagatttt ctagcacttg atcaaattcg ttcaggtaga tgttacttaa caggggagag
    25021 agattgcctc cttggggcgt tccttcttca gttgttgtga tgagtccatt ttccattaca
    25081 ccactcatta agtattgacg gatcagtttt aggacttgtt tgtcctttac tttccgctca
    25141 atgaaataca tcagcttatc gtgatttacg gtgtcaaagt aggctttcat atcaatgtcc
    25201 accacatatc ggtaaccttg ttcataataa gattttgctt tcttcactgc gtcatgtgcg
    25261 ctgcgatttg ggcgaaaccc ataactatta tctgagaatt ctggttcaaa tagcggttgt
    25321 aacacttgat tgattgcctg ttgaagcata cgatcaataa ccgtgggaat tcctagtttt
    25381 cttacaccgc cgtttggttt cggtatttct actcgcaaaa caggctgagg tttatagttc
    25441 ccttggcgta aattggatag taattcctcg cggtgctctt tcaagtaagg aagcagttga
    25501 tcgacggtca tcccatctgc accagccgcc cctttatttc tgactacttg aagatacgct
    25561 tgattgagat tattccgatc tagaattctc ccaaacagat tttctacacc atcttgtttc
    25621 ctctgttcac cgcaagaagt gctgcgcact tcatcatacc cttcgtgttc cacactagct
    25681 ctttgatgat agccaagttt tcttgttttc tgcggtcgtc gcatcttctc acacctccta
    25741 aattattcaa gattcttatt gttcagtcct tcgtaacggt cagtgtcgtt actactatga
    25801 cctcggctga cttctcatga ttcaccatca tgtcgccatg atcgttgtgc tggtttgttt
    25861 tcattcaacg cccacaacac cctcatgaga tctcccccgg taagagtgaa aactttcctc
    25921 ccatgtaact gctagattta ctgtataggt ttcgggcagt attggacttt gtcttgtgat
    25981 gaagacttat ccgacctaat tcagccttgt atctagtttc tgttcgtcag ttcaggagtt
    26041 tgcgtccgac ttccttcagc cacaacctca cgattgccgc cttgtctttc gctaacagtt
    26101 cctactgcca agcctgtagt ggactttcac caccaagttt tcacccatgc agggcgcaca
    26161 ccaaaaaaga agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat
    26221 caataatagg aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa
    26281 aaaatagagg aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag
    26341 atgttgcagc aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg
    26401 gatacgataa tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc
    26461 tttgtgttct gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc
    26521 tcggcggatt tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt
    26581 gatcgacagc aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa
    26641 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca
    26701 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
    26761 ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac
    26821 atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa
    26881 aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa
    26941 cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg
    27001 atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga
    27061 aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga
    27121 ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa
    27181 attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta
    27241 ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac
    27301 cagttggatg atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg
    27361 gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta
    27421 tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga
    27481 caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg
    27541 aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat
    27601 caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt
    27661 tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa
    27721 aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact
    27781 ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg
    27841 acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg
    27901 aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg
    27961 ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc
    28021 tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg
    28081 agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa
    28141 gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt
    28201 ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg
    28261 aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt
    28321 ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa
    28381 aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt
    28441 gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca
    28501 gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac
    28561 ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag
    28621 aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa
    28681 gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc
    28741 gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct
    28801 catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca
    28861 gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct
    28921 gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg
    28981 ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa
    29041 gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc
    29101 gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt
    29161 ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg
    29221 tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa
    29281 tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa
    29341 aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt
    29401 atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct
    29461 ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct
    29521 tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag
    29581 gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa
    29641 gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca
    29701 gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga
    29761 atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta
    29821 ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat
    29881 gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg
    29941 gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat
    30001 ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct
    30061 agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca
    30121 gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta
    30181 ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc
    30241 tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact
    30301 caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt
    30361 gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt
    30421 gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg
    30481 attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct
    30541 ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca attatttgca
    30601 gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa
    30661 atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt
    30721 tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat
    30781 gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt
    30841 ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag
    30901 gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt
    30961 tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag
    31021 ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc
    31081 cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca
    31141 atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg
    31201 atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat
    31261 taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg
    31321 caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa
    31381 acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta
    31441 taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt
    31501 gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac
    31561 cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt
    31621 tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt
    31681 cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa
    31741 atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc
    31801 gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg
    31861 attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg
    31921 caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga
    31981 ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag
    32041 aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg
    32101 gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc
    32161 cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt
    32221 tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa
    32281 gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa
    32341 aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta
    32401 tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa
    32461 ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg
    32521 tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca
    32581 acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt
    32641 gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca
    32701 ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg
    32761 acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat
    32821 tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca
    32881 tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc
    32941 tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc
    33001 actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa
    33061 atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa
    33121 attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc
    33181 caagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat
    33241 gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga
    33301 tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc
    33361 tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat
    33421 atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat
    33481 ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg
    33541 atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg
    33601 ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca
    33661 tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc
    33721 aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca
    33781 cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga
    33841 ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc
    33901 agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat
    33961 ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga
    34021 agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat
    34081 ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga
    34141 aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat
    34201 gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat
    34261 gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta
    34321 tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa
    34381 actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt
    34441 aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca
    34501 aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct
    34561 ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa
    34621 tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt
    34681 cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg
    34741 ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg
    34801 agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc
    34861 cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact
    34921 gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg
    34981 tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt
    35041 gacaaaacgt aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt
    35101 caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg
    35161 agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa
    35221 aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata
    35281 tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga
    35341 ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt
    35401 tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga
    35461 acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc
    35521 aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca
    35581 ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga
    35641 agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg
    35701 tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga
    35761 tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc
    35821 gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa
    35881 aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa
    35941 ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga
    36001 agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca
    36061 agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta
    36121 ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa
    36181 accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg
    36241 tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc
    36301 tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt
    36361 attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt
    36421 ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac cagattttct
    36481 ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg
    36541 agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa
    36601 aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat
    36661 tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata
    36721 aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg
    36781 tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag
    36841 gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg
    36901 aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg
    36961 cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat
    37021 caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt
    37081 ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa
    37141 tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag
    37201 tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca
    37261 atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta
    37321 atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata
    37381 tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg
    37441 atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg
    37501 ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa
    37561 cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga
    37621 tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata
    37681 tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat
    37741 tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt
    37801 tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat
    37861 ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa
    37921 atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca
    37981 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
    38041 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
    38101 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
    38161 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
    38221 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
    38281 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
    38341 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
    38401 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
    38461 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
    38521 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
    38581 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
    38641 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
    38701 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
    38761 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
    38821 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
    38881 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
    38941 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
    39001 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
    39061 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
    39121 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
    39181 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
    39241 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
    39301 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
    39361 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
    39421 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
    39481 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
    39541 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
    39601 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
    39661 gttgtctata actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa
    39721 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
    39781 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
    39841 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
    39901 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
    39961 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
    40021 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
    40081 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
    40141 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
    40201 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
    40261 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
    40321 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
    40381 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
    40441 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
    40501 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
    40561 cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag
    40621 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
    40681 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
    40741 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
    40801 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat
    40861 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
    40921 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
    40981 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
    41041 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
    41101 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
    41161 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
    41221 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
    41281 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
    41341 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
    41401 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
    41461 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
    41521 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
    41581 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
    41641 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
    41701 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
    41761 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
    41821 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
    41881 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
    41941 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
    42001 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
    42061 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
    42121 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
    42181 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
    42241 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
    42301 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
    42361 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
    42421 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
    42481 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
    42541 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
    42601 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
    42661 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
    42721 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
    42781 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
    42841 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
    42901 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
    42961 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
    43021 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
    43081 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
    43141 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
    43201 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
    43261 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
    43321 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
    43381 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
    43441 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
    43501 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
    43561 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
    43621 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
    43681 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
    43741 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
    43801 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
    43861 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
    43921 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
    43981 ggaatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
    44041 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
    44101 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
    44161 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
    44221 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
    44281 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
    44341 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
    44401 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
    44461 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
    44521 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
    44581 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
    44641 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
    44701 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
    44761 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
    44821 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
    44881 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
    44941 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
    45001 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
    45061 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
    45121 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
    45181 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
    45241 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
    45301 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
    45361 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
    45421 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
    45481 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
    45541 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
    45601 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
    45661 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
    45721 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
    45781 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
    45841 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
    45901 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
    45961 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
    46021 agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc
    46081 attgtttcgt ttgtatagac taaaaataat atatgttttt ttaccattcg tataaattct
    46141 ctttttgctc ttgatttttt tgcttaattg agttaaatta catagggcgc taaaaaaagc
    46201 cttgattaaa ttaataatgt tcgtgttttg ctggatgtga acatcaggag gataatgtaa
    46261 tgaaagtatt tgattacgaa gatattcaat taattcctaa taaatgtatc gtaaatagcc
    46321 gttctgaatg tgatacaacg gtcacattag gtaaacatac attcaaaatg ccagtagtgc
    46381 ctgccaacat gcagacgatt attgatgaaa caatcgcaga attcttagcg gaaaatggtt
    46441 atttctatat catgcatcgg tttgatgaag cagctcgaat tccgtttatc aaaaaaatga
    46501 aaaagcgtgg attgattacc tcgatcagtg ttggcgtcaa gaaagaggaa tattccttta
    46561 tagagaagtt ggcagaagaa tcgctaaatc ctgattatat cactatcgac atcgcgcatg
    46621 gacatgctaa ctcagttatt gatatgattc agcatattaa aaaatatttg cctgaaactt
    46681 ttgtgattgc cggtaatgtt ggtacaccgg aagccgtccg tgaattggaa aatgcgggcg
    46741 cagatgcaac aaaagtcggg attggtccag gaaaagtatg tattacaaaa atcaagacag
    46801 gctttgggac tggaggctgg caactggcag ctcttagatg gtgtgcgaaa gcagctcgca
    46861 agccgatcat cgctgacggt gggatcagaa cacatggcga tattgcaaaa tctgtccgtt
    46921 ttggtgcgac aatggtcatg attggttcat tgtttgccgg tcacgaggaa tctcctggcg
    46981 aaacaaaagt cgaaaatgga atcgtgtata aagaatactt tggttctgct tcagaattcc
    47041 aaaaaggcga aaaacgaaat gttgaaggaa agaaaatctg gatccaacac aaaggatctt
    47101 tgaaagatac actagtggaa atgcaacaag accttcaatc atctatttct tatgctggag
    47161 gaagagatct ggaagccatt cgaaaagttg actatgtcat cgtgaaaaat tcgattttta
    47221 atggagacac aatctaaaaa ataaataaaa tattaggatg ttgtgaaaaa gttgtcctgc
    47281 attaagtaat aagaacagcc aaacaaaaat agttctttat atgttttgtt tggctggaat
    47341 tattatcgca gatacaagct tttgaacatc attattcaga ataagggact gtgataagat
    47401 ttttgtcaca gtcccttatg ttttgcaaaa aaagtttttc ttttttctcg taaattcggt
    47461 aaaatcaaaa gaaaaaatag acaatagaaa agagaagctt atgtgggaaa aagtagagaa
    47521 aaaacaagga ccgatttatc gtcaaattat ggaacagatt atgagagaaa tcgaaaacgg
    47581 aaggatcaat cctggaagtc aacttgaatc agaacgaaaa ctagggcaac tttttggtgt
    47641 gaatcgttcc accgttgtcc atgcattgga agagcttagg gagcttgggg tactaacgag
    47701 caaacgtggc agcggatggt ttgttaatca gactgaatgg gggaaattcg ctgttccaag
    47761 aatcgattgg cggcaaatga tctctcctcg ttatgagcag acggactggt atgagcaaaa
    47821 aataagagaa gcaaagcaga tccaaaaaac atcgtttctt gatttatacg ctagcgagat
    47881 gccagatgag cttttgccga attttgagtt tccttcttat tctcttgaag aaatcttagc
    47941 ggaagaaaaa gaaatggatc ttttaggcta tactccgtta cgtaacaaaa tcaaacggta
    48001 tttggaaagt aaatttgaat tggcattttc ccccaaccag cttcttgtga ctggtggcgg
    48061 tcagcaagca atctttttga ttttacaaac gattctttca gctggagagg ggatagctgt
    48121 agagtcacct tctttttttt accgattacc tcttttcaaa gcttcaggaa cgcgtttatt
    48181 tgggataccg atggatgaag aagggatcga cttagaaaaa ctgaaagaat cgatccataa
    48241 aaataaattg aaagcagtgc ttatcaaccc gaattttcaa aaccctacag gaaaagtgat
    48301 gagtcaaaaa cgacgagaaa agctagtaga attgtgtcga cagtatcggc ttccaattat
    48361 tgaagacgat gtatttacgg atctttcatt tctaaaccaa gaagaagtcc ttcctatacg
    48421 aacgattgat ccggataatg tgttatacgt cggttcgtta tctagggttt taggaaaaac
    48481 aacgaaaatc ggttgggtga taggacctga gacttttatc tcgcaactag ctcaagcgca
    48541 agaaatgatg gagttttcga tgaatatcct tacacaaatc acagtggcta atgtatttaa
    48601 tgaagcaatt gatttgaaaa tgcaagatgt aagaaaaaag ttgaaagaaa aaagccgact
    48661 gataagcgct tggggagatt cacaatcgtt tttcaagctt cattcaattt taggtggtta
    48721 ctatgcctgg attacttggg atgggaaaaa actagacccc gaactagcag aaaaagtagt
    48781 agatatgggt cttggaattg caccaagtct actttttggg caagatataa atggaatcag
    48841 aatcaattac agtaggctgg atgaaaacca aataccgttg ttttattcaa agatgaaaat
    48901 tctagaaaag tggttaacag aataatttca caaagtaaat agattaaaga atataacagc
    48961 ttggatttaa atttcaagct gtttcttttt tttcggaaaa agaatacgat ataaagaaac
    49021 taagaaaaaa ccattatttg tacgtccgtt cacgatgtgt aaataaaata aatgtttgca
    49081 acaaaactgt ataacaccaa cgtttttaaa tagaaacaga tggttgtaag tttgattaga
    49141 aaatagaaaa aatgcagatg aaatataaaa cgctttcctt attgtgattt tattcactaa
    49201 tgctgttaat atgcggattg gcataatttc ctttcatcgt gaaaatttgt gcttttagct
    49261 tgtatttctc ataaatccat gataatttat taatgaagac ggttaaatag tgatacagtt
    49321 ttattcactg tttcaatcgc agaatgaaga gattttctta tagatgagag aggaatgtgt
    49381 gacaaatggc aaacaaaaaa gcaccgatcg acttccaagc actgctagat gaagtcaatg
    49441 cagatttccc agtatatcaa gcacttgatc aagatggaaa cgtagttaac aaagatttag
    49501 tacctgattt atccgatgat gaactcgttg aattaatgac tcatatggta tggtctcgtg
    49561 tattagacca acgttcaaca gcgctgaacc gccaaggacg cctaggcttt tttgcaccaa
    49621 ctgctggaca agaggcaagt cagctggcta gtgcttacgc atttgataaa gaagatgttc
    49681 ttcttccagg ttatcgggat gtacctcaac tagtaaaaca tggtttacct ttatctcaag
    49741 cttttttatg gtctcgagga catgctgcag gtaattctta tccggaagaa ttgaaagctc
    49801 taccaccaca aattatcatc ggtgcacaat atgtacaagc agccggtgtt gcattaggtt
    49861 tgaagaaacg aaacaagaaa aatgtcgtct tcacttatac tggtgatggc ggttcatcac
    49921 aaggtgattt ttacgaagca atcaatttcg ctggcgctta ccatgcaaat gccgtatttt
    49981 atatccaaaa caacgggtat gcgatctcta cacctcgtga aaaacaaact gcagcaaaaa
    50041 cacttgcaca aaaagctgtc gcagctggga ttcctggtat tcaagtcgat ggaatggatc
    50101 cgcttgcagt atatactgta tctaaaatgg cacgggaatg ggcgatcagt ggaaatggac
    50161 cagtcctaat cgaaacactg acttatcgtt atggaccaca tacactatct ggtgacgatc
    50221 caacccgcta ccgtacaaaa gacacagatg acgattggca gaaaaaagat ccattgatcc
    50281 gcttccgtaa atatttgacg gaaaaaggac tttggtctga agaaaaagaa gaacaagtca
    50341 tcgaagcaac taaagaagaa atcaaagcag ctattgctga agccgacaaa gtaccaaaac
    50401 aaaaggtttc agatttcttg aaaaacatgt tcgaagtatc tccacaatca atcaaagaac
    50461 aaattgcaat ttatgaagcg aaggagtcga aataatcatg gcacaaaaaa caatgatcca
    50521 agcaatcaca gatgccttag cacttgaact agaaaatgac gagaatgtcg ttgtttttgg
    50581 tgaagatgtt gggaaaaacg gaggagtttt ccgggcaact gaaggattgc aggaaaagtt
    50641 cggagaagac cgagtattcg atactccttt agctgaatct ggaatcgcag gattgagttt
    50701 cggtcttgcg ttagaaggat ttcgtccagt tcctgaaatc caattctttg gttttatttt
    50761 cgaagcaatg gacgaagtag tagctcaaat ggctcgtaca agatatcgaa tgagcggcac
    50821 tagaaatcta cccatcacaa ttcgttcgcc atttggtggc ggtgttcata caccagaatt
    50881 gcattcggat aatttagaag gtttgatcgc tcagtctcct ggtattcgag tagtgattcc
    50941 ttccaaccct tatgatgcaa aaggactatt gatttcttct attagaagta acgatccggt
    51001 tgttttccta gaacacatga aactatatcg ctcattccga gaagaagtgc cggatgaagc
    51061 ttacgaagtt cctttggata aagcagcagt tactcgtgag ggaacagatg tatctattat
    51121 cacatacgga gcaatggtgc gtgaagcaat caaagcagca gacaatcttg caaaagaaaa
    51181 tatatctgtt gaaatcattg atcttcgtac agttgcgcca ttagatgtag aaacgatcat
    51241 tcaatccgtg gaaaaaactg gtcgcgtagt tgttgtccaa gaagcacaac gacaagcggg
    51301 cgttgccgcg caagtagttt ctgagatctc agaacgcgct atcctttcat tagaagcccc
    51361 aatcggacga gtttctgcac ctgatacagt cttcccattt ggacaagcag aaaatgtctg
    51421 gttgccaaat gcaaaagata tcgaagacaa agtaaaagaa atcgcggaat tttaagaaaa
    51481 gaagggacga aacaaagtca tggcttatca atttaaatt
//
LOCUS       DACJGB010000012        49987 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.012, whole genome shotgun sequence.
ACCESSION   DACJGB010000012 DACJGB010000000
VERSION     DACJGB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.012"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..1008)
                     /locus_tag="IYI34_000775"
     CDS             complement(<1..1008)
                     /locus_tag="IYI34_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR0558666.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="IYI34_000776"
     CDS             complement(1188..1532)
                     /locus_tag="IYI34_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAR0558667.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="IYI34_000777"
     CDS             complement(1525..1959)
                     /locus_tag="IYI34_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAR0558668.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="IYI34_000778"
     CDS             2097..2834
                     /locus_tag="IYI34_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558669.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="IYI34_000779"
     CDS             2827..4038
                     /locus_tag="IYI34_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558670.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4245..5024
                     /locus_tag="IYI34_000780"
     CDS             4245..5024
                     /locus_tag="IYI34_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAR0558671.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="IYI34_000781"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="IYI34_000781"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAR0558672.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="IYI34_000782"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="IYI34_000782"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAR0558673.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="cpaF"
                     /locus_tag="IYI34_000783"
     CDS             7740..8669
                     /gene="cpaF"
                     /locus_tag="IYI34_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAR0558674.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /locus_tag="IYI34_000784"
     CDS             8626..9675
                     /locus_tag="IYI34_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAR0558675.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /locus_tag="IYI34_000785"
     CDS             9672..9950
                     /locus_tag="IYI34_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAR0558676.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /locus_tag="IYI34_000786"
     CDS             9925..10395
                     /locus_tag="IYI34_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAR0558677.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10412..10714
                     /locus_tag="IYI34_000787"
     CDS             10412..10714
                     /locus_tag="IYI34_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558678.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10731..11102
                     /locus_tag="IYI34_000788"
     CDS             10731..11102
                     /locus_tag="IYI34_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAR0558679.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /locus_tag="IYI34_000789"
     CDS             11074..11433
                     /locus_tag="IYI34_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558680.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="IYI34_000790"
     CDS             11605..12612
                     /locus_tag="IYI34_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0558681.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="IYI34_000791"
     CDS             12637..13821
                     /locus_tag="IYI34_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAR0558682.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /locus_tag="IYI34_000792"
     CDS             complement(14258..15841)
                     /locus_tag="IYI34_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR0558683.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16419..16562)
                     /locus_tag="IYI34_000793"
     CDS             complement(16419..16562)
                     /locus_tag="IYI34_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAR0558684.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="IYI34_000794"
     CDS             complement(16785..17264)
                     /locus_tag="IYI34_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR0558685.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="IYI34_000795"
     CDS             complement(17337..17621)
                     /locus_tag="IYI34_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558686.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="IYI34_000796"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IYI34_000796"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="IYI34_000797"
     CDS             complement(17965..19251)
                     /locus_tag="IYI34_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAR0558687.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="IYI34_000798"
     CDS             19389..20060
                     /locus_tag="IYI34_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAR0558688.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="IYI34_000799"
     CDS             20026..20514
                     /locus_tag="IYI34_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAR0558689.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="IYI34_000800"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="IYI34_000800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAR0558690.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="IYI34_000801"
     CDS             21475..22239
                     /locus_tag="IYI34_000801"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAR0558691.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="IYI34_000802"
     CDS             22472..24685
                     /locus_tag="IYI34_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAR0558692.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /locus_tag="IYI34_000803"
     CDS             24696..25142
                     /locus_tag="IYI34_000803"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAR0558693.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="IYI34_000804"
     CDS             25313..26572
                     /locus_tag="IYI34_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAR0558694.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="IYI34_000805"
     CDS             complement(26661..27608)
                     /locus_tag="IYI34_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAR0558695.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /locus_tag="IYI34_000806"
     CDS             28002..29294
                     /locus_tag="IYI34_000806"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAR0558696.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="IYI34_000807"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="IYI34_000807"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAR0558697.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="IYI34_000808"
     CDS             31187..32062
                     /locus_tag="IYI34_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR0558698.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="IYI34_000809"
     CDS             32328..34211
                     /locus_tag="IYI34_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAR0558699.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="IYI34_000810"
     CDS             complement(34286..34570)
                     /locus_tag="IYI34_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAR0558700.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="IYI34_000811"
     CDS             complement(34560..35294)
                     /locus_tag="IYI34_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAR0558701.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="IYI34_000812"
     CDS             35423..36046
                     /locus_tag="IYI34_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAR0558702.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="IYI34_000813"
     CDS             36238..37359
                     /locus_tag="IYI34_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAR0558703.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="IYI34_000814"
     CDS             37366..40494
                     /locus_tag="IYI34_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAR0558704.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="IYI34_000815"
     CDS             40513..43707
                     /locus_tag="IYI34_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAR0558705.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="IYI34_000816"
     CDS             complement(43853..44560)
                     /locus_tag="IYI34_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAR0558706.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45316..45714
                     /gene="spxA"
                     /locus_tag="IYI34_000817"
     CDS             45316..45714
                     /gene="spxA"
                     /locus_tag="IYI34_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAR0558707.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            46046..46699
                     /locus_tag="IYI34_000818"
     CDS             46046..46699
                     /locus_tag="IYI34_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAR0558708.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            46925..47923
                     /locus_tag="IYI34_000819"
     CDS             46925..47923
                     /locus_tag="IYI34_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAR0558709.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            48099..49907
                     /gene="pepF"
                     /locus_tag="IYI34_000820"
     CDS             48099..49907
                     /gene="pepF"
                     /locus_tag="IYI34_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAR0558710.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        16169 a         8740 c        10454 g        14624 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaagacaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagagga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggc
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    44761 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    44821 ttattttttt aatacagtct caatataact attcaaacat aaaataacgt tatttgattt
    44881 atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg cttatataac
    44941 agagaaaata agcgttataa taaagaacag tattatttaa aatgacagaa aacattacta
    45001 ttaaagaaat aaaaaatctt aagaaatcgg tttcatttag cttttgagat aaaataatag
    45061 tacagtttta tacgctgttt catatgttta gaaaatttga attagatttg aaaactctaa
    45121 tttttaaatg ataaaaaaag cgagaatgct ggttttactg tatattaacc ttagataact
    45181 ttcatttttt taaaaaaatt atgaaaaaaa ggtagctatt taaaagttga ttatgtataa
    45241 tagaaacaga tgggagatta ttatgatctt ctgttacaaa aaagtgattc ttatcacgaa
    45301 aaggagtggt gcacgttgtt aacattatat acttccccaa gttgtacttc ctgcagaaag
    45361 gctcgtgcgt ggttgcaaga acatcagatt ccatttgttg aacgtaatat tttttcagaa
    45421 ccattgaaca gttctgaatt aaaagcgatt ttacaaatga cagaagacgg aacagaagaa
    45481 atcatctcga cacgttccaa agtattccaa aaactgaaca tggatttaga tgatctgccg
    45541 ttacaagaat tattggaact tgtgcaaaac aatcctggat tgcttcgccg tccaatcatg
    45601 attgatgata aacgtctgca agttggtttt aatgaagatg aaattcgtcg gttcctaccg
    45661 cgcgatgtac gacaattaga attacgtcaa gcacaattga tggctggatt atagaaggat
    45721 aatacgaaac ttggaaaaga ctattacaaa gatagttttt ccaagttttt ttgtttttac
    45781 aaaaagtatt cttgattaga tgtataaaaa ggtagtagaa gatgatgatt gcacgaaaaa
    45841 tagagaggga aaattagctg aaaagtcggc tatatagtat tcgtctgcca aaaatgttat
    45901 agttaaataa gatagcttga aaggttgaaa aatccgctgg ttttattaaa ttcgcttaac
    45961 agcgagattt tttttcttta catttaagct gatttcgcta caatgaatct atattaaaca
    46021 ttgtggcaaa gtgaggtgta gcaaaatgga aatggaacat attaatgaaa atacgatccg
    46081 tgtactaatt ggtcatgaag atttagagga acggggcgtt tcatttttgg atttacttgg
    46141 caatcacaaa gaaatcgaaa atttctttta tagtattttg gaagaagttg atatcgaaga
    46201 ggagttccga ggaagcgaag ctgtcacttt ccaagttttg ccaaaaggag acggattaga
    46261 actgtttatc agtaaaaatc ttccggatga agaaatttct caatcagaag attctactgt
    46321 tgattcagaa gatgtgagtg actttcttcg tcagcagatt attggcagcg atccggagga
    46381 agaattatct ggaagcgccc tagaagaaca ggaaagacaa gcaacttttg cttttgaaga
    46441 ttttgaacaa gtgatccaac ttgcaacaga tgtcgaacta gagtctgctt ggactgattt
    46501 gtatttattc gaagagcgct attacctagt cgttcacttc tggatggaaa atctcaacca
    46561 agcggatgta gagaatcaga ttgctcgaat tttggagttt gctgaaaaga gcgatcgtac
    46621 accagaaaca ttgaatgaat atggacaatg tctgatggcg cgtaatgcta tcgagcgaac
    46681 gaaattttat tttgtataaa tcagcattat agtaagagac tgtgacaaaa attgtgtcgc
    46741 agtctttttt tcgtataaat gtgttcaaaa gcttgcatct gcggtaacaa gcccaaccaa
    46801 acgaaaaata ttgggaacta tttttgttta gctgttctta ttacttgatg cagaatagct
    46861 ttttcacgcc ctcttttttc gtgtaaataa attcttagac gtaaataagt tcagagggag
    46921 aaaaatgctg cttgcaatga atgaatataa taagtgctgt tttgctaaac atgcagaaaa
    46981 aggaaagaaa tattattgtc gtggctgtcg aaaaagagtg attttgaaaa agggaaagaa
    47041 aaaatgtgct cattttgccc atcaaaaatc agataactgt tcggtcttta gtgaaagaga
    47101 atcaaaagag cacttacagt taaaggaatg cttcatggat tggctgggac agtcagtaga
    47161 accagtattt ttggaagcct atttgccgag gttgcggcaa aggccggata ttttgttggc
    47221 gaatctagca atagaaatcc aatgttctag actgtctcac cagagattta ttgaaagaac
    47281 ccaaaattat ttgaataaca gttaccaagt atggtggata cttggtaatt cttttcttgg
    47341 acaatcgcaa ttctcattga tagaaaaaag ttgctgctac tataatagaa aaagaggagt
    47401 gcattgctgg aaagctgatc tcaagaaaca aaagctttat ttgtatcatc atattacaga
    47461 gactgtatca gggcatattt ccttttgcag tagctgctgg actttttcta gtagggacct
    47521 taaagaaata ttcactagta acgaaattaa aatgaaccaa atgaaaaaaa tagaaaaagt
    47581 gagtgaagat gggaagaaat ggctgacacg ccaacttatt cacaagcaaa agaatacagt
    47641 atccatacaa gaacaatgct acttgcgtca taaacatctt ttgcatctat catcgtggat
    47701 ctatcaaaaa agtcggtttt tcttttattt acaagaacag gtttttcttt atcgaatgtt
    47761 gtacgaagaa gccttggaaa accagaaagt tcctgatttc aactcatggc tttgccaaat
    47821 aaaagaacat aaaagggaat ggctttttcc tatgattgat gaagagatgg tctatcagca
    47881 gttttttaac gaatgcatac acttgtcttc tttgaaaaaa tagtaagtat aaaaataggc
    47941 taggaatcgg ttttctatgt aaaacaagat ttagttatgc taaaattgat ggaagcagac
    48001 acatgaatga gtagaaaagt tagatgctgt gacatgtgga tgttagacta tgatctattt
    48061 tgatagatag agctaaggga agaaaaggag tggatttcat ggaagtaaag cagttgccaa
    48121 aacgagaaga attgcctgaa aatttaactt gggacttgac caagatcttt tcaagcgacc
    48181 aagagtttga tgagaaatat ttggaattat cagaagagtt aaaacaatct gaaaaacaca
    48241 aaggaacact tgatcaaggc gcttctcaat ttttaaatgc gattgaattc gtattgaggg
    48301 tttatcgcca aactgaagtc atttatgtat atgcgcacct taaaaacgat caagacactg
    48361 gaaatacaga ttaccaagcg ctttatgcaa gagcaagcag tctgttttcg aaagttagtg
    48421 aagccgtttc ctggtttgaa ccagaaatat tgcaattgtc agatgaccag atttggcaat
    48481 atttcaaaga agaaccaaaa ttggaagtct atcgccatta tatccagcaa atagtagata
    48541 atcgagccca tgtcttatct gctgagcagg aatctcttct tgctggagca ggtgaaatct
    48601 ttgatgcttc aagtgataca tttgctgttt tgaataatgc agatctagtt tttccaacga
    48661 ttgaaggaga aaatggtgaa atagtccaat tatctcatgg cgtgtatggt cagttgctag
    48721 aaagcacgga tcgaagggtg cgcgaagcag catttaaggg gttgtacagt gtttacgaac
    48781 aatttagaaa tacatttgct tctactttag gcacacatat aaaaggacat aattttaaag
    48841 cgaaagtccg taattacagc tctgccagag aagcgtcttt gagcaataat catattcctg
    48901 aaagtgtata cgatactttg gtagacgtgg taaacaagca tttgcctttg ttacatcgat
    48961 acatggaatt acggaaacgt ttattagaag tggaaaaact gcacatgtat gatctttata
    49021 caccggtctt aggggaagct ccaattacct ttacgtacga agaagcaaaa gaaaaagctt
    49081 tagaagcact gaaaccaatg ggtgaagaat acatggccat cgtagaaaaa gcattctctg
    49141 aacgttggat cgatgttgtc gaaaataaag ggaaacgaag cggtgcttat tcttcgggaa
    49201 gctatgacac aaatccatat attttattga attggcatga tacgctggat cagctattta
    49261 cgcttgtcca cgaaatggga catagtgttc atagttattt cactcgttcg aaccagcctt
    49321 atgtgtacgg cgactactcc atctttttag cagaaattgc ttcgacaacg aatgaaaata
    49381 tcctaacgga gtatttattg gaaacagaaa aagaccctcg tgtacgggct tatgtactca
    49441 accattactt ggatgggttt aaaggaacag ttttccgcca gacacaattc gctgaattcg
    49501 agcatttcat gcatacggaa gatgaaaaag gtgtgccatt gactagtgaa tacctaagtg
    49561 atagttatgg taaattgaat gcaaaatatt atggtccagc agtcgaagaa gacccagaaa
    49621 ttaaattcga atggtcacgg ataccgcatt tttattataa ttactatgtt ttccaatact
    49681 cgactggctt ttctgctgct tcagcactgg cgaagaaaat actaaaccag gaaccagagg
    49741 cactagagaa ctacttggct tacctaaaag caggtaacag cgattatcct gtggaagtaa
    49801 tgaaaaaagc aggagttgat atgacacagg ctgcatatat tgaagatgca atgtcgatgt
    49861 ttgaacaacg tctaaatgaa ttagaagaat tgatcgatcg tttataaggc tttctatttt
    49921 tgattttctc gaatagaaac gaaaaaaata cgtcggctct cttgtaaggg agtcgacgta
    49981 ttttttt
//
LOCUS       DACJGB010000013        48564 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.013, whole genome shotgun sequence.
ACCESSION   DACJGB010000013 DACJGB010000000
VERSION     DACJGB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.013"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(23..2095)
                     /locus_tag="IYI34_000821"
     CDS             complement(23..2095)
                     /locus_tag="IYI34_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAR0558711.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IYI34_000822"
     CDS             complement(2028..2405)
                     /locus_tag="IYI34_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAR0558712.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IYI34_000823"
     CDS             complement(2534..3094)
                     /locus_tag="IYI34_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0558713.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IYI34_000824"
     CDS             3244..4536
                     /locus_tag="IYI34_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR0558714.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IYI34_000825"
     CDS             complement(4596..5714)
                     /locus_tag="IYI34_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAR0558715.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IYI34_000826"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IYI34_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAR0558716.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IYI34_000827"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IYI34_000827"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAR0558717.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IYI34_000828"
     CDS             8920..9519
                     /locus_tag="IYI34_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAR0558718.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IYI34_000829"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IYI34_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAR0558719.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IYI34_000830"
     CDS             complement(11214..11558)
                     /locus_tag="IYI34_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAR0558720.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IYI34_000831"
     CDS             complement(11695..12831)
                     /locus_tag="IYI34_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAR0558721.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IYI34_000832"
     CDS             complement(13020..13994)
                     /locus_tag="IYI34_000832"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAR0558722.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IYI34_000833"
     CDS             complement(14074..14949)
                     /locus_tag="IYI34_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAR0558723.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IYI34_000834"
     CDS             15079..16986
                     /locus_tag="IYI34_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAR0558724.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IYI34_000835"
     CDS             complement(17527..20805)
                     /locus_tag="IYI34_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAR0558725.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IYI34_000836"
     CDS             complement(21076..21621)
                     /locus_tag="IYI34_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR0558726.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IYI34_000837"
     CDS             complement(21633..22325)
                     /locus_tag="IYI34_000837"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAR0558727.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IYI34_000838"
     CDS             complement(22344..22628)
                     /locus_tag="IYI34_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558728.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IYI34_000839"
     CDS             complement(22641..23204)
                     /locus_tag="IYI34_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAR0558729.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IYI34_000840"
     CDS             23432..24094
                     /locus_tag="IYI34_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAR0558730.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IYI34_000841"
     CDS             24218..25414
                     /locus_tag="IYI34_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAR0558731.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IYI34_000842"
     CDS             complement(25515..26891)
                     /locus_tag="IYI34_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0558732.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IYI34_000843"
     CDS             complement(26928..27794)
                     /locus_tag="IYI34_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAR0558733.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IYI34_000844"
     CDS             complement(27883..28908)
                     /locus_tag="IYI34_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0558734.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IYI34_000845"
     CDS             complement(28926..29600)
                     /locus_tag="IYI34_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0558735.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IYI34_000846"
     CDS             complement(29784..30677)
                     /locus_tag="IYI34_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR0558736.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IYI34_000847"
     CDS             complement(30751..32448)
                     /locus_tag="IYI34_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAR0558737.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IYI34_000848"
     CDS             complement(32441..33754)
                     /locus_tag="IYI34_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR0558738.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37791)
                     /locus_tag="IYI34_000849"
     CDS             complement(33754..37791)
                     /locus_tag="IYI34_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAR0558739.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37804..38262)
                     /locus_tag="IYI34_000850"
     CDS             complement(37804..38262)
                     /locus_tag="IYI34_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR0558740.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38560..39078)
                     /locus_tag="IYI34_000851"
     CDS             complement(38560..39078)
                     /locus_tag="IYI34_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAR0558741.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(39080..39697)
                     /locus_tag="IYI34_000852"
     CDS             complement(39080..39697)
                     /locus_tag="IYI34_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAR0558742.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39694..40548)
                     /locus_tag="IYI34_000853"
     CDS             complement(39694..40548)
                     /locus_tag="IYI34_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0558743.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40545..41456)
                     /locus_tag="IYI34_000854"
     CDS             complement(40545..41456)
                     /locus_tag="IYI34_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0558744.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41497..42831)
                     /locus_tag="IYI34_000855"
     CDS             complement(41497..42831)
                     /locus_tag="IYI34_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0558745.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            43039..43869
                     /locus_tag="IYI34_000856"
     CDS             43039..43869
                     /locus_tag="IYI34_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAR0558746.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(44113..44362)
                     /locus_tag="IYI34_000857"
                     /pseudo
     CDS             complement(44113..44362)
                     /locus_tag="IYI34_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44553..44747)
                     /locus_tag="IYI34_000858"
     CDS             complement(44553..44747)
                     /locus_tag="IYI34_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558747.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45252..45437)
                     /locus_tag="IYI34_000859"
     CDS             complement(45252..45437)
                     /locus_tag="IYI34_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558748.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46287..46628)
                     /locus_tag="IYI34_000860"
     CDS             complement(46287..46628)
                     /locus_tag="IYI34_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR0558749.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46628..46858)
                     /locus_tag="IYI34_000861"
     CDS             complement(46628..46858)
                     /locus_tag="IYI34_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR0558750.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            47145..47483
                     /locus_tag="IYI34_000862"
     CDS             47145..47483
                     /locus_tag="IYI34_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558751.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47688..47927)
                     /locus_tag="IYI34_000863"
     CDS             complement(47688..47927)
                     /locus_tag="IYI34_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAR0558752.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(47903..48250)
                     /locus_tag="IYI34_000864"
     CDS             complement(47903..48250)
                     /locus_tag="IYI34_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAR0558753.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14110 a        10349 c         8512 g        15593 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgcggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacgtaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggaatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caaaatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccatgt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttgtt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttaatg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcgg gtagcgatgc tgttcgtttt caacttctcc
    37561 gctaggatag atatattcat aggggtcttg cgtagctgtt ggcccaagac gctccaaaat
    37621 accgttgaat aactggcgtt gaatgcgctg gagtgcttga ccgctgggta cgattggttc
    37681 gtccagcttt tccatctcat tttcttcgct acgttgaatt tcagcggaat cttctgtatc
    37741 agtttcattt tcttgtaatt cttggttatc tattctttca tcaaccacca cttgaagagg
    37801 tagttaccct tcgttcacat cggggatctt ttctgtttca aaaatcaatg tgccggtttc
    37861 tgcttctttt gaataaactt ttccgacata ccattcttga ccatcgatcg tctcgacttt
    37921 taggtcttct ccatcttcat cataggagga tagacctgtt ccacgaacgt tttttacggt
    37981 accaatcccg gcggctgccg catcgattgc taaacgtatt ctaccttcat tggcactttc
    38041 ctttttttca aaagccagat gccattcaag ggtttcttct ttttcaatat acttcccttc
    38101 aatttttgca tagtcctcat ctaagaatac ctcatcctcc tttgtttctg ttgactcagc
    38161 agctacatcc agggtcgtca tatgatagac tgactggaaa tatggtccaa atagcaaact
    38221 gacagttact aactgcaaaa gcttcaattt taaccctttc acatccttat actcctttca
    38281 aaaatatttg tcttctttca caacaatcaa caaaacaatg cccatctaat tataatttaa
    38341 tttaatttta attttaaatc aaatgatatc ctttttagtt aaaaaacacc aattatacaa
    38401 catttattat taaaattaaa ttataaaatt ttataacgtt ctcatttaac aaaacttaag
    38461 ttattaagac aaatgaataa acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa
    38521 tgcgcattta cgcactcatg ctttattcgt acaattaatt cagataacgg cagcgcatag
    38581 gatgccaata acacttccta aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag
    38641 taaaaaaatc ctcgaataag cgatcaaaca agcaaataaa aaggctaata ttcctgcaac
    38701 tggacttaat tcgaacaacg cagctactga taaaaaggct agcgctgtat gtcctgaagg
    38761 aaaagaagaa gtcttcggtt tacttgtgta aaggacaagc cgacgatttt tttcataagg
    38821 tcttttccgt cttgtcgttt ttttgataaa aagcaaacca acaaaaaaat agatcaaact
    38881 gccgataaga atggctagac catattctct agtatcaggt atcaagacta aaacagcagc
    38941 aaggagcaac caaataaaac caccattaga gatattactg ataaatttca aaaaattgat
    39001 ttttcgacta tccgttttcc gttgcttggc gaacttgttg ataatactat catcgatttt
    39061 agcaatcaaa cgcaacatgt tattcctcct ttttaagaac cttttcttgc gtacgatgga
    39121 taaaaagcag gacttgaaaa taaaacaaga aactgaaacc taataaagca aaatagattg
    39181 gtcctgcaaa ccagaatgcg acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc
    39241 ttttgaaatc attaaaaaac aagaaaaatg cagtaatcca agaagcttct ttttgttcca
    39301 gttgaagcaa gattttgtaa ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag
    39361 tatacatcaa tgtatagcca attcccccac cccaatagcg gaaaatctga taatcgatca
    39421 atagaagaag aaaataaaca cttgctaaac tatcttgctt tccataaaga gagaagttct
    39481 cacgaaactt tttccagaat agtgaaccga gctttttctc ttcatttttt ttgtaatgat
    39541 aaatcatata cagaactgtt cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca
    39601 ggaaccatag tagctgtaaa tggataagac gtggaatcca ttcaatgatt tctaagaaat
    39661 aatcattggc ttttttacca atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc
    39721 catcccttca atgaaataac gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac
    39781 aagtgttgct cctgccatca gataattcca ttgtgtcgtt gcttggttct gaaaaacttg
    39841 caagccaatc tgtaacgtgt atttttcttg atcttggata tataacagag gacctaagaa
    39901 atcattccaa gctgcgttga aggaattgat cgcaatggta atcaaggcag gtttcgtcaa
    39961 tggaagcatc aaatgactcc aaatatacaa atgattcgct ccgtcgattt cagcagcttc
    40021 gatcaattca ttattgatcg ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt
    40081 gccgaagaac gatggaacga tcaggggcaa ataagtacct acccagccga tcttggagaa
    40141 taatacatat tgagggatca tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa
    40201 agcaaacatc agctttttcc caggaaaatc aagcttcgca aatccataag caataaaaga
    40261 atttgaaagg acattaccga tcactactag cacagtgatg aataatgtat ttcttgcata
    40321 gcgccaaaaa ggaaaggcgg caatcgtatc cagatagttt tcccatacga ttttttctgg
    40381 gaaaaaggtc ggaggataac gaacgatttc ttccattggt ttcaatgaag taaacaccat
    40441 ccaaagcaat ggaatgatca aaatgattcc tattactgtc atgaggacat aacttagtat
    40501 tttttttgtt tgccgattca cactggtctt tttgaaataa tccatcattt gccgtcacct
    40561 ccagaataat agacccactt aggtgcaagc ttcaaattga caaacgtcag aaccaacacg
    40621 atcagaaaca ggatcattga catagccatc gcataaccca tatcaaaagc tacgaacgcc
    40681 ttgttccaca tatgcagatt ataaaaaagc aaagaatttg caggtccgcc tgttccattt
    40741 ttcgtcatga cataagcttc ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca
    40801 tcgtaaaaaa taataggagt gattaatggc aaagtaatac ttttgaactt tccccaaacg
    40861 cctgctccat cgatttctgc tgattcatat aaagatctag gaacattttg taatgtagct
    40921 aagtaaagta gcattgctcc tccagaactc catattttca tcaaaatcaa cgcaggtttc
    40981 gtccattgtg gatcaaacag ccaagctggt ccagggatat ggaaccagcc cagaaatgtg
    41041 ttgatcaatc ctgcagatgg agaaagcagc tgcatccata agatgtagac tgctactcct
    41101 gataagactg ccggtaaata gaaagctgta cggaatacac tgattccttt gattttttga
    41161 ttgagtactg tggctaaaaa cacactgaaa atcgtcgtca gcggtacatt gaagatgacg
    41221 aaataaacgg tgttgtataa cgatttccaa aataaatcat cgtgaacgaa cattcttttg
    41281 aaatttgcta gaccaatgaa attcatcttg gatgtcaaat tataatcggt gaaactacca
    41341 ataactgaaa aaagcatagg ccctagcata aataccaaga acccgataat aaaaggcgag
    41401 ataaataaat aggcccatct tgcttctttt ttctttcgga tagataaacg cttcaaattt
    41461 tcttgcctcc tctgtaccta gacagtcaaa aatggattat tgattttgct tgactaagtt
    41521 ttctactgct ttttgtgcat ctgccaatcc ttcttttggt gattttgttc ctaaaagaat
    41581 ctggtcaatc tgttcattca ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt
    41641 gatcaccgta tttttaaagt tttcgtccgc catttgatag atcatttttc ctttatccgt
    41701 taggttgtca ttattcacta aattctcatt ggctgtcttg ctcgccatga tatcaaaact
    41761 tttttcgccg aatttttctt gtacttctgg agaagaaaga tatttgatga attcataaga
    41821 ggcctcagga tcttttgctc cgtatggtac ttccaagacg aatccgccac cccacgacca
    41881 atggcctgaa ccttcttctt tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg
    41941 tgcattttcc atcaaactag agtaataatt gatattttgg gcacgcatgg ctactaatcc
    42001 ggaaataaat ggatcagata caccagaccc aaattcggct tctaatttat tgatcgtatt
    42061 tttcccataa taatcttgcc agtcttggat ccactccaag gcttccactt tatttttagt
    42121 atcaatcttg actttttcat ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc
    42181 ccatacatca gcccctaaat tccataatgg ataaaaaccg atgcgtgtaa attcttttcc
    42241 ttctttttta tctagtttac gtgctgtttc ttctaattca tcccaagttg tcggtagatc
    42301 ttgttcactg attccagctt ctttaaataa ctttttatta taaaaaatga cttgtgtatc
    42361 tgtattgaat gggatcgcat aaggatcacc ttcatattct actgtatccc aaagctgcgg
    42421 ataaaaatcc ttagaagtgt ctttttctac atatttggag agattagttg cttgctgtgc
    42481 ttctgctctt tgggcaacgg aatttatgtc ctgtacaata acatcgggtg catttccagc
    42541 tgttattgca gataaagatt tagtccagat gtcaccccat ggctgatact tataatttac
    42601 atggatctcg ttttgtgatt gattgaaatc ctcaatgatt tcttcgatag cttcttttcg
    42661 tgcaccggat ccccaaaagg accagaaatc aatatttttt cttccatcac ttgttttttc
    42721 agaagaattt gctgcacttg aactggcatc gcttgcatta cttgatccgc atcctcctaa
    42781 taaaaacaat gctgaacttg ccagcaaccc tgtaaacaat aattttttca tattttacct
    42841 ccaaatttta atgtaaccga tgccggtttt aatataacga tatcattttg ttctgaaaat
    42901 aacaatttat aaggtatgat tctaaaaaaa ttattaatga taccaatact ttatataatt
    42961 cgcgagcgct ttctttgaat tttttaatct ttcgtagcag aatgagaatg tattaaaaat
    43021 agattaggag gaattttgat gtttagacac cagaaagaat tgcagtttga agcaaaacca
    43081 gatcgtccga atccattaat cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc
    43141 gagatgagtg tagctatgca gtacctgttc caaggctgga gctgccgagg agaagaaaaa
    43201 tataaagata tgctgatgga cattgcaaca gaagaaatcg gccacgtgga aatgttggcg
    43261 acaatggttg ctcgtttatt agagggtgcg cctgttgaag atcaagaagc agcgatgcaa
    43321 aaagatccaa caatcggtgc cattatctct ggaatgaatc cacagcacgc gatcgtagct
    43381 ggcttaggac ctcgtcctgc tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt
    43441 gcgagtggca acttgttagc agactttaga gctaatttaa atgccgaatc acaaggaaga
    43501 cttcaagtag cacgtattta cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct
    43561 gttctccttg caagagatag ctatcatcaa aatctatggg aatgtgcagt gaaagaatta
    43621 gaagaaaaag aaggttcaat cttagtccca agtactttcc cgcgtgacaa agaacgaaaa
    43681 gacattgctt atgacttcta taacttctca gagggagacc aaagtagtga aggttcttgg
    43741 gctagtggca aagcactgga tggcgaaggc gaatatcaat ggttgaaaga acctaagatc
    43801 gaaggcgctg caccaaaatt agaaccagta acaccaaaaa tgtatgggac acctccattc
    43861 aagaaataaa tatctacccg tagaaacacc cgttttttgt tccacacgct ttgtgagcaa
    43921 aactgactaa agtcattaat cataaccacc cttcaaaagg gtggttttct ctagagctat
    43981 aagctcaaat caccggccag cgcctaaaga cgctggcttt catattttat gttcaagcca
    44041 tattgccttt gccagttttg cccacctctt aaaaggttta tgtactactc cttagccctt
    44101 aaaaggatcg tcttactctc gtacactcag cttatctaac gcactatcat gtttttcttg
    44161 ttcttgaatg tactttttga tggtcgcttc attttgtcct acttttgtca catagtatcc
    44221 ttccacccaa aagtgtcgat acccaaattt atattttaga tttgcacaat ctcttgatga
    44281 tctcctgaat attggctcga tattgattat aaattatttt tcttctatac tttggtgtga
    44341 atacaatgtg gtatttacac atctactttg tacgggctaa actatttccc tttattgctc
    44401 cacccgcata gcaagtggtt ttgtatttca atcgctcggc gcgctcaatt aggtctaaag
    44461 acaataaatc taaaaacccg ctgcttcact gacagaaaca acgggttttc agaaaaaaat
    44521 gaaatgaaaa agatctctta cggcaatcta ttttattttt ttgatttttt ataaatggaa
    44581 aatgcttcaa gaagtccaag aaataaagcc gtataaaaac aagaacctat gaaatcttta
    44641 tttattagat attcaacaga aatcccgata acactagcca acaatatatt tatcagaagt
    44701 gtaaaataag gatgcttttt atagaataga actagtgttg ttttcataga ctctcctttc
    44761 ttcatatttc caattaatca caaattcacg gcattactag aaaaattaaa ggggcgcaga
    44821 agtggaaaaa tactcttcga ctttccctac aaaccaggcc atcttgtgtt taataactat
    44881 accaaatcta aggtacctct aaatctaaat tccatttgtg gcggtgacct ccaattttta
    44941 actcttctat ctcccatcca gtatacataa ccttgatact tcgaccactt atatatcgaa
    45001 cacacaaata cctatccatt gcaggagtac tacctaactt agttgctact tgcatctaaa
    45061 taacagctga aaagatagga cttactctta gtaaagtatc tgaactatta atttttaaac
    45121 tatcattttt atcctcaact acatgcacat ctgttgcaac ttcactttct gtctcatcca
    45181 gataaatgat taaggaatcg ggaattgcaa tatcttcagc tgacgctgga atagttgagc
    45241 taaattagtc atcatatctt tattataaaa taaccaacag caaaaaatac aatgaaaaac
    45301 aaacttggaa ctaatgtatc attaataaat gttgaaaaat tcagcaactc attactgtat
    45361 gattttagca gcccaaccaa catataccct ataaaagcta ataagatggt taggaatcct
    45421 ttagaatttt tactcattca tagacacttc cttaaataac ttttattata gaaggtattg
    45481 taaataataa tattgacaaa aagtgactca aaataaaaaa aaccttacat tatactggaa
    45541 tgaactgtat tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa
    45601 gtaattttat taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta
    45661 cttttacatt cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg
    45721 cacattttcg atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt
    45781 agatttttca gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct
    45841 tttcattgtt tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa
    45901 attctttcct agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta
    45961 aaatgacatt actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt
    46021 ctttttcttc tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt
    46081 gaaaaaacaa gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt
    46141 cgtttaaaat gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac
    46201 tagtagaatg aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa
    46261 aatattatag agtttactta caatgcttaa aacatatact gtataatctg atcaatttta
    46321 gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga
    46381 gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa
    46441 cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta
    46501 cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct
    46561 acggagggat caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct
    46621 tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat
    46681 aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg
    46741 ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat
    46801 cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta acaagcatta
    46861 tcatcactcc cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg
    46921 taaactatct agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa
    46981 attgtagcca gttcattttt tctttaacta aaaaaataat ccaagaaaca ttgaacctat
    47041 ggatccaaaa gctacccaaa tactatagct aaccccccgc taccttcaat tttgcagata
    47101 aaacgctttg ggagaaaatt tcaaagacaa caggccatcc tatcatgccc gattataaac
    47161 aaaaagctgg ctattccttt tgctctgaca aatttcaatt gggaaagttc acgaaactag
    47221 caggtgaaga agtttcaact gttcggatca aagaatgccc gcttcaaata gaaacattgg
    47281 ttcaacgaat ttcaactaga caagactttg ctattgtaga acgtgagatc gtagctattt
    47341 ttgtagaaga agggatttta catgatccaa ctcatatcaa tgtggataaa tggcaacctc
    47401 ttatctataa attcagagaa tatacaactg ccaaccagtc gttaactttg aattttagat
    47461 tccaagaatt caaagagtca taatgataaa catcgttagg tataaataaa aaagactcgc
    47521 agcacgagta aaaaacaaat agaagctata cattagaaag gtcgactgtg accctataga
    47581 tgcagtatat gcagcttatg agcatttctt ctgttctctt tgtttttggg atctgtgctg
    47641 aagtcttttt cgttttttgc cgctctttct ttgaaaaatc gattatgtta taccttctcc
    47701 attactaatt caggcatctg ctgagtaatg atttttcgcg caaaagaaat atctgttacc
    47761 caaggttcga tttgttcttc agtcaagaca agcggcattc ggtcatggat tggtgcgact
    47821 gtgcggttag gttcagtagt cattatgatt gattctgcca ttttttcatc ctgatggcta
    47881 cgataaaaac cgccaatata cactattttt tccttatttg aaaacataaa tttgtctttt
    47941 tcttcattcc actcattggg aaaacacaac gattttgcaa aaatggttga tgaaaggtct
    48001 ttttttcttc tactgtctct gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg
    48061 taaatcccca cttcatgatt tccggagtga ggatgttttc ttcctctttt cttaacgtca
    48121 caacaagatt tgacggaaaa acttctccag ctgctaattc tgctgcctgc ttcggttgtt
    48181 tcgtctgggc ttctcgataa taattctgaa ggagctctga ctctagatca aaaaaataac
    48241 gaccacacat cctaatccct cccaatcttt ttttctatca tacactgcaa caagaagttc
    48301 gcaccaacaa aacgctttca atttttgtag aactaaaaaa gttgtgacaa gtgacaaact
    48361 ctccagtgaa tggtattctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac
    48421 atgagaagct gctttttgaa attgttcctt gtgactcgga gtgaaacggc aggcttacga
    48481 cctctgggta aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
    48541 atgtagaaag ctattttcac aaaa
//
LOCUS       DACJGB010000014        47223 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.014, whole genome shotgun sequence.
ACCESSION   DACJGB010000014 DACJGB010000000
VERSION     DACJGB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.014"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(28..1260)
                     /locus_tag="IYI34_000865"
     CDS             complement(28..1260)
                     /locus_tag="IYI34_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAR0558754.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1515..2084
                     /locus_tag="IYI34_000866"
     CDS             1515..2084
                     /locus_tag="IYI34_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0558755.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2262..2702)
                     /locus_tag="IYI34_000867"
     CDS             complement(2262..2702)
                     /locus_tag="IYI34_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAR0558756.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2860..3624
                     /gene="map"
                     /locus_tag="IYI34_000868"
     CDS             2860..3624
                     /gene="map"
                     /locus_tag="IYI34_000868"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAR0558757.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3656..4579
                     /locus_tag="IYI34_000869"
     CDS             3656..4579
                     /locus_tag="IYI34_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAR0558758.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4804..5043)
                     /locus_tag="IYI34_000870"
     CDS             complement(4804..5043)
                     /locus_tag="IYI34_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558759.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5048..5467)
                     /locus_tag="IYI34_000871"
     CDS             complement(5048..5467)
                     /locus_tag="IYI34_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAR0558760.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5694..6932)
                     /locus_tag="IYI34_000872"
     CDS             complement(5694..6932)
                     /locus_tag="IYI34_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0558761.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7039..7674)
                     /locus_tag="IYI34_000873"
     CDS             complement(7039..7674)
                     /locus_tag="IYI34_000873"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0558762.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7788..7880)
                     /locus_tag="IYI34_000874"
                     /pseudo
     CDS             complement(7788..7880)
                     /locus_tag="IYI34_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8016..8651)
                     /locus_tag="IYI34_000875"
     CDS             complement(8016..8651)
                     /locus_tag="IYI34_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0558763.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9163..10305
                     /locus_tag="IYI34_000876"
     CDS             9163..10305
                     /locus_tag="IYI34_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAR0558764.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10298..10504
                     /locus_tag="IYI34_000877"
     CDS             10298..10504
                     /locus_tag="IYI34_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAR0558765.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10583..11776
                     /locus_tag="IYI34_000878"
     CDS             10583..11776
                     /locus_tag="IYI34_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR0558766.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(11783..11941)
                     /locus_tag="IYI34_000879"
                     /pseudo
     CDS             complement(11783..11941)
                     /locus_tag="IYI34_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788611.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding domain-containing protein"
     gene            11981..13120
                     /locus_tag="IYI34_000880"
     CDS             11981..13120
                     /locus_tag="IYI34_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAR0558767.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13113..13913
                     /locus_tag="IYI34_000881"
     CDS             13113..13913
                     /locus_tag="IYI34_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR0558768.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13913..14740
                     /locus_tag="IYI34_000882"
     CDS             13913..14740
                     /locus_tag="IYI34_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR0558769.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14718..15452
                     /locus_tag="IYI34_000883"
     CDS             14718..15452
                     /locus_tag="IYI34_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR0558770.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15552..16418
                     /gene="rfbA"
                     /locus_tag="IYI34_000884"
     CDS             15552..16418
                     /gene="rfbA"
                     /locus_tag="IYI34_000884"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAR0558771.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16432..17004
                     /gene="rfbC"
                     /locus_tag="IYI34_000885"
     CDS             16432..17004
                     /gene="rfbC"
                     /locus_tag="IYI34_000885"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAR0558772.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17026..18054
                     /gene="rfbB"
                     /locus_tag="IYI34_000886"
     CDS             17026..18054
                     /gene="rfbB"
                     /locus_tag="IYI34_000886"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAR0558773.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18152..19003
                     /gene="rfbD"
                     /locus_tag="IYI34_000887"
     CDS             18152..19003
                     /gene="rfbD"
                     /locus_tag="IYI34_000887"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAR0558774.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19037..21070
                     /locus_tag="IYI34_000888"
     CDS             19037..21070
                     /locus_tag="IYI34_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558775.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21114..22394
                     /locus_tag="IYI34_000889"
     CDS             21114..22394
                     /locus_tag="IYI34_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAR0558776.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22604..23410
                     /locus_tag="IYI34_000890"
     CDS             22604..23410
                     /locus_tag="IYI34_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558777.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23422..24642
                     /locus_tag="IYI34_000891"
     CDS             23422..24642
                     /locus_tag="IYI34_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558778.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24632..26218
                     /locus_tag="IYI34_000892"
     CDS             24632..26218
                     /locus_tag="IYI34_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0558779.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26257..28395
                     /locus_tag="IYI34_000893"
     CDS             26257..28395
                     /locus_tag="IYI34_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR0558780.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28763..29746)
                     /locus_tag="IYI34_000894"
     CDS             complement(28763..29746)
                     /locus_tag="IYI34_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAR0558781.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30124..31515
                     /locus_tag="IYI34_000895"
     CDS             30124..31515
                     /locus_tag="IYI34_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAR0558782.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31531..32571
                     /locus_tag="IYI34_000896"
     CDS             31531..32571
                     /locus_tag="IYI34_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR0558783.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32590..33675
                     /locus_tag="IYI34_000897"
     CDS             32590..33675
                     /locus_tag="IYI34_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAR0558784.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33708..34670
                     /locus_tag="IYI34_000898"
     CDS             33708..34670
                     /locus_tag="IYI34_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAR0558785.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            34663..36090
                     /locus_tag="IYI34_000899"
     CDS             34663..36090
                     /locus_tag="IYI34_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAR0558786.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            36092..37162
                     /locus_tag="IYI34_000900"
     CDS             36092..37162
                     /locus_tag="IYI34_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558787.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            37149..38570
                     /locus_tag="IYI34_000901"
     CDS             37149..38570
                     /locus_tag="IYI34_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAR0558788.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            38583..39590
                     /locus_tag="IYI34_000902"
     CDS             38583..39590
                     /locus_tag="IYI34_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR0558789.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            39602..40606
                     /gene="neuB"
                     /locus_tag="IYI34_000903"
     CDS             39602..40606
                     /gene="neuB"
                     /locus_tag="IYI34_000903"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAR0558790.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            40603..41751
                     /gene="neuC"
                     /locus_tag="IYI34_000904"
     CDS             40603..41751
                     /gene="neuC"
                     /locus_tag="IYI34_000904"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAR0558791.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            41724..42401
                     /locus_tag="IYI34_000905"
     CDS             41724..42401
                     /locus_tag="IYI34_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAR0558792.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            42391..43533
                     /locus_tag="IYI34_000906"
     CDS             42391..43533
                     /locus_tag="IYI34_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAR0558793.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            43546..44523
                     /locus_tag="IYI34_000907"
     CDS             43546..44523
                     /locus_tag="IYI34_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR0558794.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            44523..45431
                     /locus_tag="IYI34_000908"
     CDS             44523..45431
                     /locus_tag="IYI34_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAR0558795.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            45432..46184
                     /locus_tag="IYI34_000909"
     CDS             45432..46184
                     /locus_tag="IYI34_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAR0558796.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            46189..47136
                     /locus_tag="IYI34_000910"
     CDS             46189..47136
                     /locus_tag="IYI34_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAR0558797.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        15785 a         7469 c         9073 g        14896 t
ORIGIN      
        1 attttttgca tgtgtgcgaa agtatactca agcccaatcg ccatttcgga aaatcggtac
       61 tgtagagcca tcttcacgga ttccgtcgat atccatcttg tcagatccta ccatgaaatc
      121 tacgtgggtt tgacttctat tcaatccagc ttctgctaat tcttcatcgg acatttctgt
      181 accgcctttc acactgaagg cataagcaga acctagtgct aagtgattcg atgcgttttc
      241 atcaaataat gtattgaaaa agatgatgcc agattgagaa ataggtgaag ggtcaggtac
      301 aagtgcgact tctcctaaac gacgcgcacc ttcatctgta tcaagcagtt ttgctaagac
      361 atcttctcct tgttcggcag aaaaatcaac aacttttcca tctttaaaag taaatttcat
      421 acccgagatg atggtgccag cataactcag tggctttgta cttgaaatgt aaccatctac
      481 tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc atatttgcca tgaatttttc
      541 tccacgagca ttatagcttc cagcgccttc ccaaagatgg tttttaggca atccgataat
      601 gatatctgta ccgggagcag tatagtgaag ggcagaaaat tgttctcgat tcaattcttc
      661 tgcttttttt gctaattttt catcatgctt cttccatgct aagacagggt cttcttcata
      721 gacacgagtc gttttgaaaa tctgatccca taatgcctca acttgttttt cttctggcag
      781 ttctgggaag acttttgctg cccattgttt accagccgca gccacgacag tccaactgac
      841 tttatttgct tgagttgcct tgcgtaaatt cattagtgct ttgccattcg ctgattgata
      901 agacgctact cgatggctat ctacaccagc aaatgcatcg ggatcggcag aaacaacgct
      961 gatcctgctt gctccttttt caagccattc atccgcttga tcgattttag attgtggtac
     1021 attctcaata cgatccgttg cagcatgtaa aagaaattct ctttggatct ggtcgtcagt
     1081 ccattgaacg ataacctcag cggctcctag tttataagct tcttgtgtga tcaatcgagc
     1141 aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg tgtccttttt ctgtggctac
     1201 gccagtttct gcaattaagc gtgcatattt ttttagaagt tcattaaaat cagataacat
     1261 aataaaaatt cctcctcacc attttttctg gtaactatta gcatgatacc atttaattat
     1321 gattcaaaca atgaggcttt aaataaaaaa aggaggttat ttttttaagg ttaagtttca
     1381 tgatatatgt tactacttgc tatttcagct tgtgaagaaa atcaaaaact aattttgcat
     1441 atcaaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa
     1501 ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg
     1561 cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca
     1621 aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac
     1681 aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata
     1741 cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga
     1801 agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct
     1861 cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag
     1921 agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga
     1981 tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc caagacagta
     2041 tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc
     2101 cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag
     2161 cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata
     2221 cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt
     2281 tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta
     2341 acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc
     2401 gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc
     2461 aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca
     2521 tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct
     2581 acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca
     2641 atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc
     2701 atgatgtgcc tcctcgttta taattattta acaattatga attataccaa aacatcattg
     2761 aaaagaaaat aaaacctaga gacacctggg gaatgtcatt tggctaaatg tatgccgcta
     2821 tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca
     2881 cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg
     2941 cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat
     3001 atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact
     3061 tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac
     3121 ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt
     3181 tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag
     3241 aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt
     3301 catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt
     3361 catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga
     3421 aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga
     3481 acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt
     3541 tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca
     3601 caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa
     3661 agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa
     3721 gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt
     3781 gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca
     3841 agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg
     3901 tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt
     3961 agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc
     4021 gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat
     4081 tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat
     4141 tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac
     4201 tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat
     4261 tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt
     4321 cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat
     4381 cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc
     4441 tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca
     4501 tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt
     4561 ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttatt
     4621 tgaaatttta tatctaattt tcaattatcc gcataaacac tatacattca agcattttga
     4681 agtatagaaa aacactcatc ttaccacgaa tttaccacga ttgaatgagc cttgatatga
     4741 ccgtaaaact actaagggag atagcacgaa actgtgtatc tcccgatttt tttatacagt
     4801 tgattacttc actccgattt caaatttcaa tattgctttc atcatgtctg tatgaatttc
     4861 tccttttagt tcttcatcaa cctgcataat gattttacca ctttcagagt ggtaaaaagg
     4921 acgcaagcat aatttagaaa tataaccgtc ataaaacttc aaaatctctt taactgccat
     4981 ttcatctccg cttgacgctt tgcaaatcaa ttcaaaagag ggatagccat aatctttact
     5041 catttcttta cttcctttcg ctcaaaagca tttttaactt ccttagtcct ctagttcttc
     5101 tactgcaaat agcagaacgt gatacatggt acagttctga aatttctctg tcgttcattc
     5161 cttgaaaata acgtaataaa atcgcactac gctgtttttc tgacagtcta tgtaaaactg
     5221 taccaagtgt ttcatcagat acataaattg taaagtgcaa gactttaaat gatacacaat
     5281 cataaaaaca ggtatcaatc gttccacgtt caacttgttt cgtttcggct atttcacaaa
     5341 acaatacttc acgtttagaa tgtcttgcaa gctgtctgtt tcgacttttc actttgttct
     5401 taatgacaat cagcattaaa gaattgaact gtaatctgac gatatgctcg aaagaagatg
     5461 tcctcataat ctcaccccct ttctgctaat gctgaaatat ataaacggaa taccgtttat
     5521 accctttaga aatacagaaa aatggtaaga aaaaagccca cacaaaacat atatttgtgc
     5581 gagccttaaa tactgcaata ttactctttc ttttataaca ctatcctttt agcagatttc
     5641 aaattttctt ttatatattg tttttagcct aacatactat ttaacccctt gtattatatc
     5701 tctaactttt gaaaaattct ttttgaatat attgaaatca tgattgaagc aatagaagct
     5761 acaaacataa caacccatgt ttcttgtata aatacatcga ataaaatacc gtaaaccatt
     5821 tgtccaatag gttgaccgca catagcaact gctaccagcg tagccattat ctttcctact
     5881 aattgcggtg gtgtttgtgt ctgaacaaga gtgtaaattt gaacaacaaa catagtagat
     5941 acacccatag ccacaaagca catcactgta actacccagt aggaaactat tgcaggcaat
     6001 tctaaaaata gcgaaattcc cataagtaaa acagaaacag aacaaataag taacaaggta
     6061 taaaggtttt ttagttttaa ctttgaagca acaagagctg ttaaaatacc accacataaa
     6121 ccacctaatg ataatgctga ttgtgaaaaa ccatatagtg tatctggcat ttttaaaact
     6181 tcagaaatta agataggcag accaacaatt aaaacagcac ttaaaaccaa attgaaaatg
     6241 caaaccaaaa agattacgga tataaaaatg ggtttctcct ttttcaaaaa ggtataacta
     6301 tcttttaaat cgtttttagc aactgcaatt acacctactt cacgaggtct tttttggtgt
     6361 ggaatatgga taaaaatttc cataatggct gaaaaagtaa aacatataat actaacaatt
     6421 aaaatgggat tgataccata aaattgaaaa actactccgc ttatagctgg tccgataaag
     6481 tttgctaaag tatttacttg atttattata gcattaccga cggtaagttt atctgatgat
     6541 accaatagag gaatacttgc ttgtaccgct ggttgatatg caccagaaat accataaagt
     6601 aacatcaaag caacaataaa caaaggtatg ataggaattt ttcccaatga caaataaaat
     6661 actaaaatta tgattgctgt tacaaaatct agtatgacca taatgtttct tttatttgtc
     6721 ctgtcagcaa taataccacc taccagcgac aaaataacca taggtaaaaa agagcaggct
     6781 gtagcaatac caaacaaagc tgatgaacca gtttctttta acaaatagta cggtaatgca
     6841 aatcgtagga tagcgtttcc aaataaagaa attatctgtc ctataacaac aagtataaaa
     6901 tcacgattaa aaagtttttt ttctgcttgc ataaaatctc tcctttttat ttaatcaaac
     6961 agaccgtcgg tttatataat ggtttaaata aactgctctt ttatagagca gtctatcgta
     7021 tagggataac aaatacagtt atttacaaaa ctgtataaat gcagaaattg tttcttcgtc
     7081 taataatgtg tctatgccta ttccatttaa aagggcattg aatgtattgc aacgtttttc
     7141 cataccctct ttatccgtca tttcaactaa tgggttgagc catacattac ttaataccat
     7201 gataacttct gcaatttcat gtgaattttc aatctccaaa gacttatcct tgataccttg
     7261 ttctaaaacg ggttcaacaa attcaggtac tacaatttca tacatttgtt ctatttgcat
     7321 agcaagaaaa cgaggattgt ttaatagttg aggagaaact gtcaataaaa cgctttggtt
     7381 agaattgaat accgctgtct taaatatttc tcttaacttt tctaaaccgt ttaaactatc
     7441 gttatctcta attttagcaa atattactgc attttcattt ccaattcttt gaaaaattgc
     7501 ttttaaaata tcttcctttg aatcgaaatg atgataaata gcacctttag aaagttttgt
     7561 ttcattgata atatcttgca atgatgtgtt atcatatcct ttttcacaaa acatttttgt
     7621 agaaacatct aaaatgagtt ttactgtttc ttctggatat ttatttcgtg ccataactta
     7681 cctccaatac aaaccgttgg tctgattaaa gtatatctta gtattttatg cttgtcaata
     7741 taaagacttg caattttata aaaatgcaga atgcaagcct ttttcattta ttctacaatc
     7801 ttccagttgc tttccttatg cggtgtcaat tcatactgtg atacctgcgt tgcctttgcc
     7861 tgatttacca caaatcagat tttatccctt tcttcctctt gattgcccac aacagaaaag
     7921 aaaaagccct gtcaagagct ttgccaccat tggtggtgct ttgcactctt gatcgggctt
     7981 tttgtttgct gtatcttctc gcaagaggaa aaaagttact cgtcctcatc atcaaaattg
     8041 ttattcctta atacttctcg taacagttcc atatcttctt ttgagttcgg gtttatcatt
     8101 ttcacgactt gataaggtgt cttatactta tgtccctcta tgctttcccc aaacatatcc
     8161 atactgtata aatcatcata gcggtctaat aatttttgaa acttcaaatg ctggataaat
     8221 tctttgattg ggtaaaggtc ggacgcttca atgcttttcc cgttgatata gtctgtaata
     8281 tattcccgta acttttctaa ctcatattcc agccattctt cctcgctgtc aaagaagtta
     8341 tcatagtgtc catgtttcag ttctgcattt agacgttctt cggctcttaa aaactgacag
     8401 acttcctttt cagattgatt gacatacttc cattctcccg acaacaattc attgggcgta
     8461 acgtccagca cttccattat cttttccagc gtttcatagg caggatagtt caaccctctt
     8521 tcaatcttgg aaaggctctg catattgata ccgattttgt ccgcaagttc ctgttgtttc
     8581 attcccctaa attttcttat ggtctgtata ttatttccta aatggcttat tttcattgca
     8641 taaccctcca tatctcgtta tatcttcgct attataagtc tatcatgctt aatatgattt
     8701 gtaaaggcaa aaaaagatat tgacaaataa gcatataaga attactatgt tcttgtaatc
     8761 gaataagcac aatagaatga aatataaaaa cattcaaaaa gaaataccta tctgattaaa
     8821 tccgtagata tgaatatcgg ttgaaatgga acttgttaga aaaggcaaaa cagaacttgc
     8881 tgacaaagta tttctgacac ggtggcggaa gcctctcagg cttaccgcca tgacaccttg
     8941 tgtcaagggg aagaaatgct ttgtcggctg gcgagcctgc gagccttgtc tgtgcccccg
     9001 ttatctaaca ggggggcact atttcacaaa agacaagtca aaaaccatac acccccaaac
     9061 ctgtgagtag taatgagagg ttttaagaag atagtacata tcacactttt tgaaaggtgg
     9121 tttacaggta tttcaaggct attgtgggat tggaggaata caatgaacaa tttagaattt
     9181 gcacttgaaa tgaagaacaa acgtcttgcc agcggtcttt cacaaggaga acttgcaagt
     9241 cttacccatg tatcaagata tagtatcaac cgctttgaga acggaaaagc taacgccagc
     9301 aaggaaacac agaacattat cctgcgttgt ctgaattact atatctgcga taagcctttt
     9361 tatctgctcg ttgattatct gtctgtccgt tttccaacga ctgacgcatt ggaagttatc
     9421 cgaaaggtgc ttggcatgaa agcagattat tttatccatt atgactatgg gtattatggc
     9481 tataaggagc attatgctta cggggaaatc aaggtcatgg cttctgatga tgaacacatg
     9541 ggcgtatttt tagaattaaa aggggcaggc tcacgcaaca tggaatatgt cttacaggca
     9601 cagaacaggg actggtattc attcttaaac cgctgtcttg actgtggcgg tgttatccga
     9661 cgttttgact tggcaatcaa tgatatgtgc ggtctgctgg atattcctgt gctatctgaa
     9721 aaatacagaa atggtggtgc ggaatgtcgc tgtaaaaact atgaaaatgt acaaggtgga
     9781 aaattaagcg gaaaaaaccg taatttagca agtacccttt atatcggctc aaaggcaagc
     9841 acaaaatatt tctgcctgta tgaaaaacaa aaggaacagg caacgaaaaa gaaacatacc
     9901 gatattatca accgttttga aattcgtctg cgtgacaaaa aggcggtaca ggcagttgaa
     9961 gaactgttat tgacgtataa ccctcatggg ctggtgtttt atctcattac cgattttgtg
    10021 gaatttcctg actatccatt gtgggagata ttcatttccc atgacagttt accttttgaa
    10081 atgaaccctg tacctgtcaa tatggaacgc actctgcaat ggctggaaag acaggtcatg
    10141 ccgtcaatcg tgatgataga ggaaatcgac aggctgacag gttcaaacta catgaaaatg
    10201 attgatgaat gtacccacct ttctgaaaaa caggaaatgc ttgtggaaca gatgtgtacg
    10261 gacatatcag acgtaatcga aagtgagggg gtgttttatg agtaatacac aggatattcc
    10321 cgtatgggaa aaatacaccc ttaccattga agaagcgtca aagtatttcc gcattggaga
    10381 aaacaagtta agacgattgg cagaggaaaa caaggacgct ggctggctca ttatgaatgg
    10441 caaccgcata cagattaaac gccgacagtt tgaaaaggtt attgataaat tggacgcaat
    10501 ctaatgcaaa tgagccttgt atgtgttatg atgaacacaa gtcatatcaa ggctctttcc
    10561 aacaaggaaa ggagcagaca ccatgaaaga aaaaagacgg gataacaaag gtcgtatcct
    10621 gcatactgga gagagccaac gaacagacgg aaaatactta tataaatatg tagacgcatt
    10681 gggaaacaca aaatatgtgt atgcttggag attgacaccc acagacccga caccaaaggg
    10741 aaaacgggaa aaaccctcac ttcgagaact ggaacagcag ataagacggg atattgagga
    10801 cggtatcgac agcacaggca agaaaatgac gctttgccaa ctctatgcca aacagaacgc
    10861 acagaggaca aatgtgaaga aaagcacaca gaaacaacgg gaacaactca tgcggttatt
    10921 aaaagaggac aagttaggtg ctaggagcat tgatacgata aaaccctctg acgctaaaga
    10981 atgggcgtta cgcatgaaag acaaaggctt ttcctacaat accattaaca accataaacg
    11041 ctcgttaaaa gcgtcattct acatagccat acaagatgat tatgtgagga aaaacccttt
    11101 tgatttcaag ctaagtgaag tcatagaaaa tgataccaaa gagaaagtcg cattgacaga
    11161 ggaacaggag caagccttat tgtcatttat taagacggac aacgtgtatc acaagcatta
    11221 tgatgatgtg ctgatactgt taaagacagg acttcgtatc tcggaactgt gcggactgac
    11281 agtagccgac attgatttca agaatgaagt tgtgattatc gaccaccagt tactaaagag
    11341 taaggaacag ggctattata ttgaaacgcc taagacgaaa agcgggataa ggcaagtgcc
    11401 attaagcaaa gaaacgatac aggcatttca acgggttatc aacagccgac caaagataaa
    11461 gtcgatagag gtagacggac acagcggttt tgtgtttatc aatccgaaag gtaatccaaa
    11521 agttgccatt gattataacg ccttatttat ccgtatggta aagaaataca acaagcacca
    11581 caaggacaat cccttgccac atattacacc gcatacgcta cgccatacat tctgcacaag
    11641 gctggcaagc aagaacatga acccgaaaga tttacagtat atcatggggc attcaaacat
    11701 cagtatcaca atgaactggt acgctcatgc gtccatagat accgcaaaat cagaggttca
    11761 gcgtctaatc gcatagaagt atttaccacg attttaacca cgattgacag ggaaaatata
    11821 agaagataga cctagatatg cgaggtttac cacaaaagca aaatgcccgt agagccgata
    11881 aaataaggct ttgcggacat ttgagaagat ataaaaagat agtcaaaaag acatatataa
    11941 tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt cttttagtat
    12001 ggttataaga ttttttctga caattttatt atcactgata ttaacacctg tattcaaaat
    12061 catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa ataaagttcc
    12121 aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga ctttattctt
    12181 attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt ctttagtaat
    12241 catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa aaagtatagg
    12301 gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg attccttcac
    12361 gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa cgattttttg
    12421 gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc tggcggcagg
    12481 gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc tccatgcttc
    12541 tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg gtttttttcc
    12601 ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat tcctagggtt
    12661 tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt catcgatttc
    12721 tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca ttaggagaaa
    12781 agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc gactaatcag
    12841 tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt tgatgttttc
    12901 tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat tgattattgc
    12961 catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg agaaacatca
    13021 gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc aagtaaaaca
    13081 gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac cagccgatcc
    13141 ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat aattttattt
    13201 caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg
    13261 agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc tattttcatg
    13321 atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc gattctcgct
    13381 atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca gcattggtct
    13441 tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt aatgaagatg
    13501 gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg
    13561 tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt gaatgccgag
    13621 gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt atggtagctg
    13681 atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg tactttgaag
    13741 acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac acaccctttt
    13801 atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt
    13861 ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc tgatgaaacc
    13921 agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc cagcttatgg
    13981 accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg ataatagtcc
    14041 tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc atgatccgca
    14101 aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg aaggaacacg
    14161 ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg aactattgcg
    14221 taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt ctgggagccg
    14281 acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag cagtagaagt
    14341 tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa
    14401 atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt tggatcattg
    14461 gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg tacgaatgga
    14521 acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc gttctatttt
    14581 acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca gtcaccgacg
    14641 acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg
    14701 gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta
    14761 tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt ttatctcagc
    14821 tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat acatgggata
    14881 tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg cctcgtcaag
    14941 gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta atctttttgg
    15001 cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac tattttgagc
    15061 agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc gagctgcaag
    15121 agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt aataatgtga
    15181 taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa cccgatattt
    15241 tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg ggaggaaaga
    15301 agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta aacgcgagtg
    15361 agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg
    15421 cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc ttaatatttc
    15481 catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat caaaaaggag
    15541 agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt ctgtaccctt
    15601 taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg atttattatc
    15661 caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca acgccacaag
    15721 atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg cagatcgaat
    15781 atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga gaagaattta
    15841 ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt ggcggtctgt
    15901 ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt ggctatcatg
    15961 tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa gctttgtcga
    16021 ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt tatttttatg
    16081 acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga gaactagaga
    16141 ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa cttatgggac
    16201 gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca tctaccttta
    16261 tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa attgcttacc
    16321 gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg aaaaaaaatc
    16381 aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca gatgaaagta
    16441 acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc
    16501 ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt gaaatatgac
    16561 tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg gcttcatttc
    16621 caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc agttttagat
    16681 gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg atttatctta
    16741 tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg attcgttact
    16801 ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc agaagcagat
    16861 gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga tccatcaaga
    16921 gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga agcagaaaat
    16981 ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa aaacatcatt
    17041 gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt aaacaatcat
    17101 ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa agagaacttg
    17161 gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga tgcagaatta
    17221 gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga atcccataac
    17281 gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg tacgtataca
    17341 cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac tgacgaggtt
    17401 tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga aggagaaaaa
    17461 ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa agctggttca
    17521 gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt
    17581 tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca agtaacgaat
    17641 atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg tgactggatc
    17701 catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag aattggcgaa
    17761 acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga attgatctta
    17821 gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc aggacacgac
    17881 ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa gcctgagttt
    17941 acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca tgaagactgg
    18001 tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca ataatacaga
    18061 gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt
    18121 ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag gaaatgggca
    18181 actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg tttcaacaga
    18241 tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca cagaattaaa
    18301 acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag aagaagaagc
    18361 aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac ttgcagcaaa
    18421 agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg gaaaaaacaa
    18481 aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg gtcgtacgaa
    18541 attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca tacgaacttc
    18601 ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc tagcggaaac
    18661 ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga cacgtacttt
    18721 ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt atcatttgtc
    18781 gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag atacagatgt
    18841 agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc
    18901 agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt ggcaagaagc
    18961 acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt ttatcaaata
    19021 gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag aaaagccaaa
    19081 agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc gctatcttat
    19141 tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt cacttggtgt
    19201 ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga tttttggaca
    19261 gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc tctcacaagc
    19321 tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa acatgatcat
    19381 tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca actggggatt
    19441 ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat tagtcgtcat
    19501 ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag ctctatcgct
    19561 aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca tgcaacatgt
    19621 gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt atttctacaa
    19681 tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt tcggtattgg
    19741 gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc tgatttttta
    19801 ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt tggatgtagt
    19861 gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct ggctaacgtc
    19921 aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg taagtactgg
    19981 aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa ttccttttga
    20041 agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt ttccaactgt
    20101 attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa agttactggg
    20161 acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg ggttgccaaa
    20221 atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca tccttacttt
    20281 tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga gagaagatgt
    20341 tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt atttctatgc
    20401 gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg cagttgctgt
    20461 gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt tttacctcgc
    20521 ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag aaggtacagg
    20581 agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac aagatcctgg
    20641 taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg gagtgaaatc
    20701 cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg atccgaaaga
    20761 agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg ctaaggaaga
    20821 tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt ttgaagacgc
    20881 agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt acaaccaatt
    20941 atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata tgatttttaa
    21001 agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa atcagctagt
    21061 cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg agagtggaga
    21121 taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc ttgcttttgt
    21181 tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca actttgttta
    21241 tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc attttaaatg
    21301 tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc cttttcattt
    21361 tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta ctgtttcctt
    21421 tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg gatcgcggta
    21481 tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta tttacagaga
    21541 tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct gatatttgga
    21601 tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc agtggatttg
    21661 ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt gttgcagctt
    21721 ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt gtttttgcga
    21781 tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt attgcttaca
    21841 cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct gaaaaagtca
    21901 atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta ggagaaaaat
    21961 ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac caatttttag
    22021 gaaaccacaa tagttataat tctattttga atatctatgg attgatagga tttgggattg
    22081 ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat ttatccaacg
    22141 ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt atggaggata
    22201 cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg gccgttcatc
    22261 gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca tatgacgata
    22321 tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct tccaggatcc
    22381 aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc gcagcctttt
    22441 ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag aaaatcttgt
    22501 atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata tggtaaaatg
    22561 gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta aagatttgta
    22621 tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt cgatcaatga
    22681 cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta tcaatccgct
    22741 atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac aaatgatcga
    22801 tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt tcttcttctc
    22861 agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag taaagaaagt
    22921 cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt acacccactt
    22981 attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc cttcagcaat
    23041 gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag gattgacttg
    23101 gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta cgatcgtgct
    23161 gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg attcaggtct
    23221 tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc gtgagtcatt
    23281 tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct gggttttcac
    23341 gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac atttttcaga
    23401 tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga tatcacaaaa
    23461 tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa tccttttaag
    23521 aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat aaaaaaaggt
    23581 gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt gaaaatcatt
    23641 acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat ctcagctttg
    23701 ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat ctatttaaat
    23761 ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga tatcattgaa
    23821 tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag cgggatgttc
    23881 gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat tgtggatgaa
    23941 gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt tacagaaatc
    24001 aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat tcgtcgtttt
    24061 tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga tacgatggaa
    24121 gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt ggatcggaat
    24181 aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa agcggaattg
    24241 ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact atacgttaaa
    24301 gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat tgcgctacga
    24361 acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat caagattcct
    24421 tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt gttagctggt
    24481 gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt ggtatataag
    24541 acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat cgtggtctta
    24601 gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa tggacctaga
    24661 tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta aagttttaga
    24721 gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac aatgtgaagt
    24781 gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg ctcaagatgg
    24841 attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac agacatttga
    24901 ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa cattgaagaa
    24961 agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta acttggcgca
    25021 taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt atggtctcct
    25081 agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt ttgaaaaagc
    25141 gggattattc attaatattg aagattattt gtacttagct gtaggtgata cagaattaaa
    25201 atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc gaccatttgg
    25261 tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa gttccattgc
    25321 agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa cgaaacaaga
    25381 tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga cactaagttt
    25441 ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac ctagctttat
    25501 cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga atgctgtagt
    25561 caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt tcgttctaac
    25621 ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg gggaattgac
    25681 accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca caaagcagct
    25741 ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg aaaatacaag
    25801 actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg aaagtgatga
    25861 atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa aaatccaaga
    25921 aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa aaatattaaa
    25981 aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata agttatcagc
    26041 tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca atcaagcaga
    26101 acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg agcaagaacg
    26161 ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg aaatgtaaat
    26221 aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga aattgccgta
    26281 tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt gggctgggca
    26341 attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa tgtgattgct
    26401 gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga tgttaagacc
    26461 caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat cttagacttc
    26521 caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata cccttatgat
    26581 gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg gataaattat
    26641 gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt gaagccagga
    26701 tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa agagcaaagg
    26761 aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc tgtctataac
    26821 gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac gtatgatcat
    26881 tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa gtgtctggaa
    26941 tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa tggacacatc
    27001 agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc actcttagat
    27061 aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct aaacgtacat
    27121 ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg caatcgcttt
    27181 gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa ttatatctct
    27241 cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg taaaggctat
    27301 gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc tgcagatcat
    27361 atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc aactgcaagt
    27421 aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac agatgccctg
    27481 tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt ttatgagatt
    27541 gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa gaatggctat
    27601 gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc aaattatgaa
    27661 atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt tgcagaatac
    27721 aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt caattattct
    27781 cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt cttgaataac
    27841 gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc acagtttgat
    27901 cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca gcatgcagga
    27961 gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga tcgaacacat
    28021 tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac tgcagcttgt
    28081 atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac attagaagta
    28141 gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta caatgtctat
    28201 gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga agatactcca
    28261 gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg gccagcatat
    28321 attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg cgacttttcg
    28381 ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc gcacataacg
    28441 gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa aataagtcga
    28501 ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa attgcttttc
    28561 aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca ccgtttattc
    28621 gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt attcatgctc
    28681 agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct gtcaaagaat
    28741 cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat tcctctacaa
    28801 aagccactct atcaattatc atgggtttaa aataagaagt gatggagata accagattgt
    28861 tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat atttcgttct
    28921 cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct tctaaagcac
    28981 tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt tttcctttat
    29041 acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc gataaacaca
    29101 aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg gttttaggag
    29161 acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa ttgacatggt
    29221 ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt gttagcttac
    29281 ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag ttgaattcgc
    29341 cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa tcttcacttg
    29401 aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt gttagcaagt
    29461 gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac tctggaaaat
    29521 aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca atgccaatca
    29581 gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct tttttataac
    29641 cattccattc atctgtataa acttctaccc catccttata tgcgacaatt tgacatatat
    29701 ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga tattacctca
    29761 ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga ttcttcacgt
    29821 aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc tgaaaaatat
    29881 aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc tttaatttac
    29941 caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt gtcttgatta
    30001 gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg cttataaact
    30061 aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat taggagtgta
    30121 tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat attaatggat
    30181 gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg tggagagatc
    30241 ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt gatttttatt
    30301 gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag tgacatcgtg
    30361 tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt gacttttgcg
    30421 ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct cggtacgctt
    30481 ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag tcgagataaa
    30541 aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa ttttggttca
    30601 aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta tgcaaacatc
    30661 aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga agaaactgaa
    30721 aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt gaatacaaca
    30781 tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga aagtattatt
    30841 gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt gtttgatatc
    30901 atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac agcaatttta
    30961 gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac aaaaaatcaa
    31021 aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc aaaatctgga
    31081 ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt tattcgagct
    31141 gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat gtcgatcgta
    31201 ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa cccttattac
    31261 tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta tggaaaatat
    31321 gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa atattcattg
    31381 atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa aatgtcctct
    31441 caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt tccgaatgaa
    31501 aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag cataatcgta
    31561 ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa agaattggat
    31621 tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac acaggaaatc
    31681 attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac caatcaaggg
    31741 cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct cttattcttc
    31801 gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt gctagaaaag
    31861 agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga cgagggaaga
    31921 atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca cgaagagttt
    31981 ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg gaacaaatgc
    32041 tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa tagttgtgag
    32101 gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat gaacccaaaa
    32161 gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata ccgacctaac
    32221 aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga cgatattaat
    32281 aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt ttctatttat
    32341 agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat attgagtgat
    32401 ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa aaaaataaca
    32461 aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt tggaagcttc
    32521 gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta gttaatggag
    32581 gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg tggagcagag
    32641 tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt tgattttttg
    32701 atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag attaggaggc
    32761 cgtatttttg ttgtacctaa tcctaagaaa aatattttta aatatttaat agaagtgaat
    32821 aagttactaa aaaaagaatc tttttctata gttcacaatc aggtttattt tggcggggga
    32881 attaatttat ggttagccaa aaagaatgga atcagacaga gaattgctca tagccatgca
    32941 actgaagatg gaaaatctca aaatattgta atgaatgtat tgcgtaaatt tttaaccaaa
    33001 ttgttgttac aaaatgcaac agactacctt gccgttagtc aagaagctgg tgaaagtctg
    33061 tttcaaaacc acccctttga gatagttcat aatggtattg atttagagct ttatagtaaa
    33121 aattcagaag ctaaggttaa taagagaaaa gaattggata tctcaatgag tacttttgtt
    33181 gtgggaaaca ttggacgatt agagaaacaa aaaaatcaat cttatttgtt agagattttt
    33241 ttagaaatac taaaaatgaa ttctgattct tacttgattt tagtaggtga agggtcactt
    33301 agaaataaac tgaaagccaa agcagtagaa ttggatatag agaaaagagt aaaattttta
    33361 ggagaaagaa acgatattcc cgagttatta tctgtgatgg atgtttttgt aatgccttct
    33421 ctttatgaag gtctgccaat ggttggatta gaagcacaag cagcaaaatt aaaattagtc
    33481 ttagcagata ctatttccaa agatacaaag ttaacaaata atgtggattt tgtatcatta
    33541 gatgaaacac cacaagaatg ggcaaaaaaa gtaataaaag agccgtatgc taataaaata
    33601 acacaagaac taaagagtta tgatgtttcg tacactactg aacaaatgga gagtatatat
    33661 agtagagatc attaaaaatg ttcaagatat tttaggagtg attatttttg aaaatatgtt
    33721 atttgatatt ggcatacaat gatattggca acttaaagag attggtcaat cgattgaacg
    33781 aaaatgcagt ttttattatt catatagata aaaaaactga tatagaacca tttattgaag
    33841 catttaaatc atatgataat gttgaatttt tatgtaataa aaaccgtgtt aaaatttatt
    33901 ggggtggttt ttccattgta caagcagaaa tcaatttagt aaagagagca ttacaaaatg
    33961 agaaatattt aaaatatgta ttattatctg gtgctgatta tccaatcaaa gataatgagt
    34021 atatttataa ttattttaaa aagaatagtt cagtggagtt tattcgtgga atagatctag
    34081 atcaaattaa acataaagaa ttttattata aacatataga tgtatatcaa aaacatgatt
    34141 atcctagaat taataggaat aacacaactg catttaaaat ttttagagct ataattaatc
    34201 gttgtctgag gatgattaaa cttcccccaa aaattagaca ccataaattt gatttgtatc
    34261 atggttctca atggtgggct ttgtcgaaag aatgcctaac agaattaata caaatgtatg
    34321 aacaaaatca acaagactac ctgaatttta aaataggtat gtttgcccca gatgaaaaat
    34381 tttttcatac actttttttt aattcctcgt tcaaaaataa aaatgttatt ggaggaccag
    34441 atttaccact tgaattgaaa aatattgaag aaactacttt acaaacttcg aaattagcaa
    34501 atattcatat tattgatcct tccatgaaca aatggtttga tgaaagggat atagatcaaa
    34561 taaaagaaag tgaaaaatta tttgtaagaa aagttaaatc ggattactct gataaactat
    34621 taaatagaat tgataaagaa attttgaaag ttggaaatat agatgaatag aatagtacct
    34681 gttttagaga agagtttatt aagtcgagat gcgtttcgag taataaattt aatcatttgt
    34741 ctgctttatt ttatgcctag tacttttaat gtaactaata tcccgatgaa aatagctttt
    34801 gcttggggtg gaattatagt tttgtatgag gtttttataa agaaaaattt ttttaaaatg
    34861 cgtttttctt ggctactgat tggagcaatg attagtttta tcttaactat aatcattaac
    34921 agagaaaatt ttttaattcc atctatttat aattttggat atttgctgat tactttgata
    34981 gtaatttttc ctgcagactt ttcacaaagt gaagaggaaa aaaagaaaaa aatggtgtat
    35041 tttaataatg ttttcattat cataattttt ttagctgcgc taatatctat ctatcaattt
    35101 atttttttga tttcttatca tgtcccaaca ggaacgccag gattacttgc aagacaaggt
    35161 tttattgaac atcggttatt tggtgtctat acaagtccaa atgttggagc tatgtttgga
    35221 tatacttcta ttattttaag tttaattagt gtattaattt caaaaataac aaaaacgatt
    35281 cgtgtttttt atatgatgaa ttttatcgta caaatatttt atattacact ttcttcgtct
    35341 agaggtgccc aggtagtaat ttgttctttt ttagtattat ttcttttgtt gtttgccaat
    35401 tcaaaattta gaaagagcat tagagaagta ctacctgtaa aaatcaaatg gatggtaagc
    35461 atattattag ttctattggt atatttttct tttggtacta attatgctaa ggatttttta
    35521 gcaacaattc ctgtatcgat tgatagaaaa gtagaagtta tagagtcaaa tgaatcagat
    35581 aaaactccac ttccagaaaa agaagtaata ttacagcatt ctagtgataa ctccgaaatt
    35641 tcagctggtc gttttacgat ttggaaagct gcgcttaaag ttataagacc aactttgtta
    35701 tttggttatg gagagaccga tttctatggg aatagcgatg caaaattttt gaaaaaagta
    35761 gatcttgata ctacagatgt gaatgaacta aaaagagcac atggaaacat gcataatgga
    35821 tttcttcaag tacttgtatc aagtggtata attgctttta tatgtatcta tgctttctat
    35881 gtgttaaata tctttagttt actcaaaaca ttttttagaa gtaaagtgaa ttatgcgttt
    35941 cttggtattg ttttaatttt tatactatct atttttatag atgaaatggt ggaggctcat
    36001 gtacttttca ataagcgcga tgtcatttca attgttttct ggtattattt gggaagcatt
    36061 tcttacttgt atttaaaaaa ggagaattaa tatgaagaca gctttcattg cttggactcc
    36121 tttacatttg attaatatat taaatgttta tcaaaatttt taccgagaat caaaaggaga
    36181 tttatttatt tattctgaat ttgaaaacgc tagaagatat agtgagcgta taaaagaatt
    36241 agggctattt aatgatgttg tttttgtgaa tcaccggaaa atcggttctt cctttaaacg
    36301 taaaattaac ttgttaacta accataatca atttttctcg gatgatttat ccgtttatga
    36361 tgaaattttt atacaggggg aaaattattt cacaaaaata cttttctcca atgtttacaa
    36421 aaaaaatcca gatgtaaaac taaattatat agaagatggt ttgggtactt atttgaagaa
    36481 gagtatattt tcaacaagta aaaacgttca aagagttttt aaaatattaa ataaaaatag
    36541 catcttcaaa aaaaaaattg acgattattt tgtctattgt ccggaattgt tactttatga
    36601 tgtaaaaaat ataaaaaaaa ttcccaaaat agatgaaaac agtaaattga tgaaaaacat
    36661 acaatcaatc tttgggatag aagaagtttt tgtcggagat aaatgtatct tttttgatca
    36721 acctttatta tctgatggtt atggcataga tgaaacagat attttcgcta aagtaaatag
    36781 tatattttct ggaaaaaaag aaactttaat taagcttcat cctagagcaa ggacaaatcg
    36841 ttatggtgaa aaaaacatat tgaattccag tttaccgttt gaagtcttgc tacttgaaaa
    36901 tgacttctct tcaacacttt tgattagtcc tatatctaca atttcttttt caccatctat
    36961 gatgtttgaa aagacgatca actctattat gttggctaaa ttaattttaa atgaatatac
    37021 agcattatct gaagataaaa aagaaacatt gacaaatatt gttcttttct gtgaacatta
    37081 taaccgtcta tcagaagaaa aaattttact cccagaaaac tgggcagaat taagaaagtt
    37141 ggtagagtat gggattattt aacacacttt ccaagtcaaa attagttaag tccagtttct
    37201 ggtatactat agggaatttt tttattaagg gaatttcatt cataactatt cctatttttg
    37261 taaatattac tactcaagca gaatatggtt tgattaataa tttcacttca tttgtatcaa
    37321 ttttttcgat tcttgttggg ctaagcctaa attcggcaat taataatgca agttttgact
    37381 ataaagatga cataaatgaa tttatgtcat cggtactttc tctagctaca ctttcgttaa
    37441 ttgtgttctt attaataggg aacatatatt ttttgttttc aaatgctttt tttgatatga
    37501 accaacttgt atttaatttg atgttattac aaagttttgc tagttttgta attagttata
    37561 tccagtctta ttttacgatt aactttaacc attttaaata tttgatttta tcttttatat
    37621 ctactttttt gaatattgca atctctgtag ctttgatgca gacattatat tttgatgata
    37681 aatatgttgg cagagtagtc ggcggaacac tgtcatttgt gttgataggt ttagtaattt
    37741 atttaagaat aatgataaag ggtgctcatt tttttgataa aaaacagtgg agatacgctt
    37801 taaaaatctc tttgccgcta atcccacatt ccttagcaaa tatagtttta gcacaattcg
    37861 atagaattat gattaattct tatttaggtc ctgctgcggg gggaatatat tcctacatta
    37921 gtaacatagg tattattcta agcgtaatat ggatgtctac taataacgcc tgggttccat
    37981 ggttctatgg tgagatgagt aaaaataatg aaaaaaaagt gaagaaagtt agtaactatt
    38041 atttagtagt ctttactttg attacgatga ttgtaatggt agtttcgatt gatttagcaa
    38101 aatttatggc tccgaaagac tattattctg ggttatcttt agtggttccg ataaccttag
    38161 gctatttctt tcagttttta tatagtttac cagttaatgc tgaattttat gagaaaaaaa
    38221 catcgtttat tgcgattggt acagtagcaa gtgctatagt taatatacta ttaaacatga
    38281 ttttcatacc taggttagga ataattgctg ctggatacac aaccgtattt acgtattttt
    38341 tactttttat ttttcattat aatatcgcta aaaaaattac gaataaacaa ttatttgaca
    38401 caaaaaaaat atgtatcata ataatttttg tatcaatttt ttctttgata cttcatattt
    38461 taatcaatca aatgttgatt cgttacgttt tgacatcatt attgataatt agtttagtta
    38521 tatatttttt aaagattaag aatcgaatta tagatgacta taattcatag gaggagatat
    38581 aagtgtttaa aggaaaaaca atattagtaa ctggcggaac tggttccatt ggaagtgaaa
    38641 ttgttcgtca gcttttaaaa tatgaaccaa aagctatcag aatttatagt agaggtgaag
    38701 aaaaacaatt ttatatgcaa caagagctct caaatcacac taatttgaga tttttaattg
    38761 gtgatatcag agactctcaa aggattgatt atgcatgtcg tgatgtggat attatttttc
    38821 atgctgccgc aatgaaacac gttccagcaa gtgaatataa tcctatggaa gctgtaaaaa
    38881 caaatgtgat gggaactcaa aatgtaatag atgcagcgat caaaaataat gtaaaacatt
    38941 ttgtaggtat cagtacagat aaagtagtga gtcctactaa caccatgggt gcgacaaagt
    39001 tattgagtga aaaattaata atagctgcca atcagtataa aggaaaaaca gaaacaatat
    39061 tttcttgtgt aaggtttgga aatgtcatgg ggtcaagtgg atctgttatt cctcttttta
    39121 gtaaacaaat aaaaaacaat aaagatatta ctttgacaga tgttgatatg acacgattta
    39181 tgatgtcaat accggatgct gcaagattag ttctgtctgc tgctatgaat tcaaaatttg
    39241 gagaaacata tgtcttaaaa atgccggcaa tcaatatttt aactttagct caatcattaa
    39301 taaaaatata taatgataca catgatgaaa aatatagagg tgaaataaaa gttgtaggta
    39361 tcaggccagg agaaaaatta tatgaagagc taatgactac agacgaaatt gaacgctcat
    39421 atgaaaatga taaaatgtat atcattccac cacactatca tgctactgac gaactgtaca
    39481 aagattatga aaaaattaaa atcactgcat actcttcaga tgcaacagaa ttaatgagtg
    39541 gacaggaatt tattagaatc gcaaaagaga ataatgtcgt gacttggtaa ggagttttat
    39601 gatgaaatca gaagtatatg taattgcaga aattggtgta aaccataatg gaagtctaga
    39661 tttagcttta gaaagtattg ataaagctaa agaatgtgga gctaacgcgg ttaaatttca
    39721 aacatttaaa acgaataagc tagtttccaa aaaggctcca atggcaaaat atcagaagga
    39781 taatcttaaa gaggacagtt ctcaatttga aatgctaaaa aaattagaat tatctcaatc
    39841 tgattttatt agaataaaag attattgtat acaaaataaa attgattttt tgtctactcc
    39901 ttttgatgaa gaaagtgcag actttctaaa acaaattggt gtacaaggat ttaaaattgg
    39961 ctcaggagat ttaacaaatt taccttttct tagaaaaata gatatgtatg ggttgccagt
    40021 tttattatct acaggtatgg ctgatattca tgaggtcagt gaggctgtag attgtttcat
    40081 agatagtcca gttactattt tacattgtac ttcaaattat ccggctagtt ctgaagatat
    40141 taatttattg gcgattaaca cattaaaaga aatgttcggg atacccgttg ggtactccga
    40201 tcattcattg ggatatgatg ttggtatttg tgctgttgcg atgggtgcta aggtaattga
    40261 gaaacatttt accttggata aggaactacc tggtccagat cataaagctt ctttagatcc
    40321 tcaggaattt gcagattttg taaagcatat tagaaacact gaaatatttt taggtgatgg
    40381 tgttaagcga gcaatgcctt ctgaacttag tacaaaagaa gtatcaagaa aaagcctagt
    40441 ggtcactgag aatttaaatg tgggagatat tcttacagag aaatctctta caataaaacg
    40501 tcctggaaat ggaatagctc ctaagttttt atatcaatat attggcaaaa aagttaaaag
    40561 accaataagt gcagatagtg ttctgcttga ggaagatgtt gaatgaaaaa aatacttata
    40621 gtaagtggaa cacgagctga ttatggaata tataaacctg ttgctgagaa tttactacaa
    40681 gataattggg atgtatcatt tgttgtttct ggaatgcatt tatcccataa atatggattt
    40741 actaaagaat tgatcttagc tgacggattt agaattagtg gagaagttca aagtttgttt
    40801 ttagagaaca ataagggaaa catggctcgg tctgtggcat tagagataaa tggtttcaca
    40861 aatatatttg aacaaactag ccctgatttt gtattattac taggggatag aggagaaatg
    40921 ttggcagctg cgatagcttg tttgtatctt ggaatcaaaa cagcacatat tcacggaggc
    40981 gaagtgtctg gtactgtaga tgaatcaata agacatgcca taacgaaaat agcatctgtt
    41041 cattttgctg ctacggaaaa aagcaaagag cgtttaataa aaatggggga agattcttgg
    41101 agagtttttc ccgtcggagc tcctagaata gatactattt taaataatag attacctagt
    41161 tttagagata taaaagaaaa atatcatcta aaatttgaaa agaaaaaata cgttttgcaa
    41221 gtatttcatc cagttgtaac cgaactagaa aatatagaaa aacaaatttt agctttgatc
    41281 aattcaatgg aacaacaatc tcaaccaatt atttgcattt tacctaatag tgatgctggt
    41341 agcgaaatta tcagaaaaat gtaccagaaa tatttaaaaa cggatattat ttatattacg
    41401 aatttagagc cggaggaata cctgactata ttaaaagagt gcaaatttat gatcggaaat
    41461 tcttcttctg ggataattga agctgcatca ttcaaaatac cagtaataaa tttaggaacc
    41521 agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa tgaaaatgaa
    41581 attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt gaaaaatgtt
    41641 aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt gaatcaaata
    41701 ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta aaatatacta
    41761 gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt gaaaccttaa
    41821 gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa atgcaagatc
    41881 tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa aaaaaaatct
    41941 ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata aaaaaattga
    42001 atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg gcaaaagaaa
    42061 tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca gatgctgtaa
    42121 ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt agaattaata
    42181 actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta ggtgtaggaa
    42241 ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct tggtgtatga
    42301 taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct tatggcaatc
    42361 cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt atcaagtcct
    42421 catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa ttggatagcg
    42481 ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac tggttctaag
    42541 ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt aagactgctt
    42601 aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc tacagtaaat
    42661 cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa tacatggaat
    42721 atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa ccaattacct
    42781 aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg aattttagct
    42841 attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt aggttctact
    42901 tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt taacggtaat
    42961 aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa atatgttgag
    43021 cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca acatagcgaa
    43081 ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg acaaatggaa
    43141 gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa ggaagctttt
    43201 tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc aaatcgttgg
    43261 ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa cgtaataaat
    43321 aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga aaccactaca tttgcaacca
    43381 gtatttcaag gggtaaaaat gtattaccat gataataatc ttagtgtgtc agaaaagtta
    43441 tttaatagcg gcttatgctt accttctgga agtaatatga cagatagtga acaagaattt
    43501 gttattgatg agtttaaaaa aatattcaag taggtgtgat attttatgaa cataggaatt
    43561 attggtttag gttccatggg gaaaagaaga cttagactca ttcaagaaca ctttaatgat
    43621 attttactat gtggtgttga tactaatgag gcgagacgaa aggaagttga aaaggaattt
    43681 ggaataccaa cttataatag tctaacagag gcaaaaaatt tgtttgaaat aaattcagtt
    43741 tttgtttgta catcccctat aacccatgaa tttattataa aagaggcttt aaatttagat
    43801 tgtaatatct ttacggaaat caatttatta aatgaatatt atgatgaaat aatcgaagta
    43861 gctaaaaaga aagatagatt actttatcta tcttctacat ttttgaagag aaaagaaatt
    43921 caatatatta agtctaaagt gacgcataat aaaaaaattt cttatcaata ccatgtagga
    43981 cagtatttac ccgactggca tccttgggaa gattatcgca atttttttgt ttcaaacaaa
    44041 aaaactaatg ggtgtagaga aatattcgct attgaattac cttggattat agatacattt
    44101 gggaaagtta aatcgtttac tgtaatgacc cagaaattat ccaatcttga tattgattat
    44161 tcggacagtt tcactcttat tattgagcat gaaactggta tagttggaac tttaagtgta
    44221 gatgttgttt caagagtagc taaaagaaat ttaactataa ttggtgaaaa gattttaata
    44281 gaatgggaag gtaacccgga ttctctattt tcatggaatg aagaaacttc tagtatgaca
    44341 gatattcaat tgtacgatga ggttatgcat gataataatt attcacatac tattattgaa
    44401 gatgcatatt tagaggagat taaagaattt ttcacgcttt tggaaaagaa aatggaaccc
    44461 caatatactt ttgaaaaaga taagtatatt attaacttaa ttaacgacat tgaggtaaat
    44521 taatgatgaa agtaattttt tttgggttag ggtcaattgg aaagagacat ctaaaaaatc
    44581 taataagcta tggaaaaaag aattctatta attttgatat aacagcttat agtagcagtg
    44641 gaaaaaaaga gtttgaagaa ataacgtata ttcaaaatag agacgatttg gatacacatt
    44701 atgatatagc ttttattaca aatccgactt ttaagcatct ggaaacacta aatgtaattg
    44761 ttgaaaaagt tgattacata tttttagaaa aacctgcttt ttctgagagt attgatctta
    44821 ctcaatataa ttatgattac aaaaatattt atgttgctgc tccactgagg tataaaaata
    44881 tcatggaagt tgttcgtaat agtattcaaa gtaaaaaagt atattctgta agagctattt
    44941 gttctactta tcttcctgat tggagaaaag gtactgatta tagaaataat tattctgcct
    45001 ttaaatctat ggggggagga gtagaattag actgtataca tgaattggat tatattataa
    45061 acctatttgg tttccctaaa tcagtaaaaa aacagtttgg taaatattcg aatcttgaaa
    45121 ttaacagtaa tgatttagca acatatatat tgagatacga tgataaaatt atagaaattc
    45181 atttagatta ttttggaaga gttcctacaa gaaaatttca attgtttact ttagatgaat
    45241 gtatcaatgt tgacttatta aataataaaa taagttttga agaaaacatt gttaaagaaa
    45301 tgcctgaatc tgctaatgat atgtatgaga aggagttgaa atatttcttt gaaaacgtag
    45361 taacaggcaa agaaaactgg aatgatttat atcatgcaaa cgaagtattg aaaatagctg
    45421 aggagaaata aatggaaatt ttatttacta tttgtggtag agctgggtct aaggggttta
    45481 aaaataaaaa tttgaaatat ttggcaggaa agcccctagt ccatttcgct ttagcaatga
    45541 tagatatcta tcaacaaaaa catcctgaac ataattgcac gacttgtttg aatacggaca
    45601 gtaatgagtt agtagaattg acgactaagt taaatccgtc tgtgaaaatt gtagaacgta
    45661 aacctgaatt agctacagat actgtaggga aaattgaagt gatacgtgat tcattcttac
    45721 aactagaaag agaagataat atatttgatt taattataga tttagatttg acttcacctt
    45781 tacgtgaagt ttctaatctt gaagaattaa tagatgaaca tctaaaaaat ttagataaag
    45841 acgtctactt ttcagttgtt tctgcaagac gtaatcccta ttttaatatg gtaaaaagaa
    45901 ttgaaaatga agttactata gttaataaat caagatacac atctaggcaa cagacacctg
    45961 aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta aaaaaaagta
    46021 attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta attttagata
    46081 ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt ttagaagatt
    46141 cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat gagctgttat
    46201 cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt aggagaagaa
    46261 catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct tgatatgacg
    46321 aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga agagatcatg
    46381 aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt ggcagattca
    46441 gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat gattttagaa
    46501 ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc agcggctgtt
    46561 tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc attgacgcct
    46621 tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca tttatatgat
    46681 gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa tccgaattct
    46741 ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat cgtaggagaa
    46801 gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt ttatatagat
    46861 gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg gaaacaattc
    46921 aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat caatgaaatt
    46981 ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat ccatgactct
    47041 ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg tattttaagt
    47101 ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata agattagagg
    47161 gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa atctgggata
    47221 gaa
//
LOCUS       DACJGB010000015        45761 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.015, whole genome shotgun sequence.
ACCESSION   DACJGB010000015 DACJGB010000000
VERSION     DACJGB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.015"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(125..1933)
                     /gene="uidA"
                     /locus_tag="IYI34_000911"
     CDS             complement(125..1933)
                     /gene="uidA"
                     /locus_tag="IYI34_000911"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAR0558798.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(1956..3722)
                     /locus_tag="IYI34_000912"
     CDS             complement(1956..3722)
                     /locus_tag="IYI34_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAR0558799.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3735..4529)
                     /locus_tag="IYI34_000913"
     CDS             complement(3735..4529)
                     /locus_tag="IYI34_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAR0558800.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4539..5915)
                     /locus_tag="IYI34_000914"
     CDS             complement(4539..5915)
                     /locus_tag="IYI34_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0558801.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGAAL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(6035..6682)
                     /gene="eda"
                     /locus_tag="IYI34_000915"
     CDS             complement(6035..6682)
                     /gene="eda"
                     /locus_tag="IYI34_000915"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAR0558802.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(6766..8193)
                     /gene="uxaC"
                     /locus_tag="IYI34_000916"
     CDS             complement(6766..8193)
                     /gene="uxaC"
                     /locus_tag="IYI34_000916"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAR0558803.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8267..9346)
                     /gene="uxuA"
                     /locus_tag="IYI34_000917"
     CDS             complement(8267..9346)
                     /gene="uxuA"
                     /locus_tag="IYI34_000917"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAR0558804.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9349..10980)
                     /locus_tag="IYI34_000918"
     CDS             complement(9349..10980)
                     /locus_tag="IYI34_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAR0558805.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11284..11955
                     /locus_tag="IYI34_000919"
     CDS             11284..11955
                     /locus_tag="IYI34_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0558806.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            complement(12680..13015)
                     /locus_tag="IYI34_000920"
                     /pseudo
     CDS             complement(12680..13015)
                     /locus_tag="IYI34_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13112..13564)
                     /locus_tag="IYI34_000921"
     CDS             complement(13112..13564)
                     /locus_tag="IYI34_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558807.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(13571..14119)
                     /locus_tag="IYI34_000922"
     CDS             complement(13571..14119)
                     /locus_tag="IYI34_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR0558808.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(14164..14496)
                     /locus_tag="IYI34_000923"
     CDS             complement(14164..14496)
                     /locus_tag="IYI34_000923"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558809.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(14510..14740)
                     /locus_tag="IYI34_000924"
     CDS             complement(14510..14740)
                     /locus_tag="IYI34_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558810.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(14962..15504)
                     /locus_tag="IYI34_000925"
     CDS             complement(14962..15504)
                     /locus_tag="IYI34_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558811.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(15525..16616)
                     /locus_tag="IYI34_000926"
     CDS             complement(15525..16616)
                     /locus_tag="IYI34_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAR0558812.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(16624..18834)
                     /locus_tag="IYI34_000927"
     CDS             complement(16624..18834)
                     /locus_tag="IYI34_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558813.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(18850..21378)
                     /locus_tag="IYI34_000928"
     CDS             complement(18850..21378)
                     /locus_tag="IYI34_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAR0558814.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(21430..21840)
                     /locus_tag="IYI34_000929"
     CDS             complement(21430..21840)
                     /locus_tag="IYI34_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558815.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(21853..22071)
                     /locus_tag="IYI34_000930"
     CDS             complement(21853..22071)
                     /locus_tag="IYI34_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558816.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(22068..23060)
                     /locus_tag="IYI34_000931"
     CDS             complement(22068..23060)
                     /locus_tag="IYI34_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR0558817.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            complement(23139..24050)
                     /locus_tag="IYI34_000932"
     CDS             complement(23139..24050)
                     /locus_tag="IYI34_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR0558818.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            complement(24069..24413)
                     /locus_tag="IYI34_000933"
     CDS             complement(24069..24413)
                     /locus_tag="IYI34_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558819.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            complement(24433..24989)
                     /locus_tag="IYI34_000934"
                     /pseudo
     CDS             complement(24433..24989)
                     /locus_tag="IYI34_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25070..25570)
                     /locus_tag="IYI34_000935"
     CDS             complement(25070..25570)
                     /locus_tag="IYI34_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558820.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            complement(25571..26116)
                     /locus_tag="IYI34_000936"
     CDS             complement(25571..26116)
                     /locus_tag="IYI34_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558821.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            complement(26113..27369)
                     /locus_tag="IYI34_000937"
     CDS             complement(26113..27369)
                     /locus_tag="IYI34_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAR0558822.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            complement(27730..28041)
                     /locus_tag="IYI34_000938"
     CDS             complement(27730..28041)
                     /locus_tag="IYI34_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558823.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            complement(28064..29374)
                     /locus_tag="IYI34_000939"
     CDS             complement(28064..29374)
                     /locus_tag="IYI34_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAR0558824.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            complement(29988..30440)
                     /locus_tag="IYI34_000940"
     CDS             complement(29988..30440)
                     /locus_tag="IYI34_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558825.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            complement(30442..30807)
                     /locus_tag="IYI34_000941"
     CDS             complement(30442..30807)
                     /locus_tag="IYI34_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558826.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            complement(30906..35189)
                     /locus_tag="IYI34_000942"
     CDS             complement(30906..35189)
                     /locus_tag="IYI34_000942"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAR0558827.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            complement(35336..35575)
                     /locus_tag="IYI34_000943"
     CDS             complement(35336..35575)
                     /locus_tag="IYI34_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558828.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            complement(35665..35937)
                     /locus_tag="IYI34_000944"
     CDS             complement(35665..35937)
                     /locus_tag="IYI34_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558829.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(36020..36271)
                     /locus_tag="IYI34_000945"
     CDS             complement(36020..36271)
                     /locus_tag="IYI34_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558830.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            complement(37267..38178)
                     /locus_tag="IYI34_000946"
     CDS             complement(37267..38178)
                     /locus_tag="IYI34_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558831.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKASRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            complement(38374..40209)
                     /gene="lepA"
                     /locus_tag="IYI34_000947"
     CDS             complement(38374..40209)
                     /gene="lepA"
                     /locus_tag="IYI34_000947"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAR0558832.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            40356..41576
                     /locus_tag="IYI34_000948"
     CDS             40356..41576
                     /locus_tag="IYI34_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0558833.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            41596..42090
                     /locus_tag="IYI34_000949"
     CDS             41596..42090
                     /locus_tag="IYI34_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAR0558834.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            42083..42754
                     /locus_tag="IYI34_000950"
     CDS             42083..42754
                     /locus_tag="IYI34_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558835.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(42905..45514)
                     /gene="clpB"
                     /locus_tag="IYI34_000951"
     CDS             complement(42905..45514)
                     /gene="clpB"
                     /locus_tag="IYI34_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAR0558836.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
BASE COUNT        13804 a         9486 c         6709 g        15762 t
ORIGIN      
        1 aaaaaactag aaaaaaaaca taaaaatatt tatcattctt taaacagaaa atctttctat
       61 aaaaaacaaa aaaaaacgat taggctaggc taatcgtttt ttgaacattc ttaaaaaatc
      121 caaattattt tttataactg aaattgggta ttgatgacca tcgtttctgt agatagtaag
      181 caatcatttt aggttttcta tctctagtaa agattccttt cttatttcct tgcactctta
      241 aaatcccttg acttgttgca aaatctgcaa aattccacac ttgttcacca acaaaatttt
      301 cacatgtatc tattacttcg tgatttgctt cgtaataagc cacttgatac tcttctgtaa
      361 acattgttgg tactgtatca tgaaatccag ctactgtatc tgctccatat tctgtaaaca
      421 ttattggcgt atcaggacat ctttccatcc atccatctag ctcttttttc aacagatatt
      481 ttgcatgtgc cagatcccct cccgaaacat accaaccata ataacgattt aaacataaaa
      541 catcgacaat atctcctacc ttacaaacat ctggcgttga ttttagataa gtaacgattg
      601 ttacaggtct tttctgcgga tcaagttctt tagttaattc aactaaaggt tcaaaatatt
      661 ctttagcacc ttcggaatcc gaatctggtt catttgcaat agaccacatt acaacacttg
      721 ggtggttctt gtcgcgttct atcaattcct tgatcaccgt tttatgagaa ttttctgttc
      781 gtatctcttt ccacgtatta tgtttttcca cattatcatc taataaagtg gccatgaaat
      841 ttaaatgtaa ccctactgca ggtacttcat caataactac aataccttct cgatctgcta
      901 atcgcataat ttcttctgag taaggataat gtgatgttct aaaagaatta gcacctatcc
      961 atttcattaa attaaaatct ttaatattta cagcctcatt caaaccacgt ccattaacat
     1021 acgaatcctc atgcttacca aatcctttaa aataaaaagg tttattatta atcaaaaatt
     1081 tgccattggt aacctgaaca ctgcgaatac caaattcttc tgtataggta tcgatcactt
     1141 tattatcatc aattaaacta attttcatac tatataaata agcgtctaga ggttcccaaa
     1201 aaattggatt cttaagctga atcattcctt tctttccttc agcttcagct acaagttgag
     1261 cctcttcgtc atatatcgct attaagattt ttccggtcca ttctgtatcg atattgaatt
     1321 ctacacttgc atccaaatca gttattcttg tattaatagt aatatcactt atatagtctt
     1381 tcggagttgt atataactta acaggtctat gaatacctgc ataattgaag aaatcaaaat
     1441 taactaagtt ttcctttttt atatccccat tttcctgtac tgtttccttc ataatcccca
     1501 caggtaaagt actatcgtca ataatattgt ttacagcaac tgtaattcga ttattcccgt
     1561 cttttacaaa ccgattaatt tcagactcaa atggtgtaaa cccacctttg tgttgagtaa
     1621 taaactttcc attcaaaaaa acttttgctt catgtgttgc agatccaaaa cgaagaacta
     1681 atctttcttc taataatctt ttaggaagag tcacttcttt ttcataccac acccatccaa
     1741 catgatttct aatgttctct gtttcaaata aatcattata agaagaggga actgaaatcg
     1801 gataactctc tttttcattt attggttcta aagcaagttc ttcctgtaag tcatcatttg
     1861 tatctaattt aaatctccag agaccgctta agtcaattac ttgcctcgtc tctgtcataa
     1921 tgggatacaa catataaacc actcctatct aaatattaaa taaatgtatc ctttaaacca
     1981 caataagcat ctattggact tactccctta aattcagaaa gacccattag tttgtccacc
     2041 aatatttcta aagtttcttg ttttccatca taagtattaa tataagtctt tacttgaggt
     2101 acatcagcca aatgaaacgg acactgtaca gatacaaaaa ttgttggaat ttcatgaatg
     2161 taaaacggta tatccggagt tcctttactt gctggccaga caattcgttg aactgtgcct
     2221 ggattaacat tagctatatt aatgatcaag tcatattttt tagttaaatt tttaattggt
     2281 ctcttagcag catatattcc agctatattt ttaatttttt cttcttgcga taatcctttc
     2341 aattcttctt caggtgaaat gtatttttca acttcaaatc cttttgctga caaaatctgt
     2401 ttcaataaat caatggcttg aggtctattt ccagcaacta cttcaccaaa cccacctttt
     2461 accccatgta catctactag taaaatcctt ttatattttt ctggtacaat aggccaaata
     2521 tcttgtttat ctttaactaa tgtaattgct tggtcagcca cctctttaaa aattgattta
     2581 ttttcaatta aaccaatttt ttccagagct tcttgcttag gtaataaaat ttgttctttt
     2641 gcttttttgt gtaatcccaa tttagctttt aaccctaaaa ttctttttaa ggcatctaat
     2701 aaccgatcat cagtgataat tcctttacga tagccttcca tcatataatt aaagtcttca
     2761 tctggatcat taaaaaatag aaatagatca catccagctg caatagaagt gggcaataat
     2821 tctgatcgtt tcattgcccc agtcattcct aacatatgac tagcatcagt aactactaat
     2881 ccattaaatc cgagttttcc tctaagtaag tcagtgatta attcttttga taaagttgca
     2941 ggaagtaaat cttcatcttt cagatcagga ttaaaatgtt ttgaataagc tggcatatgg
     3001 atatgacctg ccataattga aggtaatcca gcttctatta agcctgagta tacttttcca
     3061 aaagaattat cccattcttc cgtacctaaa ctattaatac taaaagatag atgctgatct
     3121 cgttcgtcaa taccgtcacc agggaaatgt ttagctgcag gcaaaattcc actctcctga
     3181 atacctttca tataagctaa actaagttct aaaactttat cagggttttg tccaaaagat
     3241 cgactagaaa tgataggatt tctccaattt ctattaatat ctacaatagg agcaaaactc
     3301 cagttacatc caatagccga tgcttcaatc ccagaaactc tccctaattc ataagcatat
     3361 ttcgcatcat ttgttgcacc aatttttact tctacaccaa cttcagtacc atctgtacaa
     3421 gctccgttac cacctgcttc cgtattagca gcaattaata gaggaatttt actttctgtt
     3481 tgtaagatat agttttgatc atatacctca tcagcagttc ctggattata tcgtacagct
     3541 ccaatatggt actgattgac catattactc aagtaatttt tatcacgact agatcccata
     3601 ttaacaaaca attgacctat tttttcttcc aaagtcatag aatcaatcgt ctcttcaatc
     3661 caagaaatag catcttcatc taaattatat ggtttttttc gtaaatctac ttttatagtc
     3721 attttctttc tcctctactt attagtaaga tttttccaaa aagtatctat atatttttgt
     3781 tcaatatcta cataatctcc cgataaacat ttaaataaaa catcagttct taagttttcc
     3841 catgattctt tttctttatt gaccaaaata ggataaaaga aaactttatt tttatttgca
     3901 gcttctagat caccaggaga atcaccaatc attaatatct tatccaaatc acaaccattt
     3961 tcaattacac ttcgaataat atcttctttt tttcctttat cttgacaaca cagttcatca
     4021 actaatgata ataattcatg tctttcccat tcttcttgaa cagcttcctt attagctgag
     4081 cttacaataa ttatcttccc atattttttc agagctttta tagtatctct agcacttaaa
     4141 aagggtttat ctaatccttt tgcttgacta atctgttgat tgacttcttc cgaccatttt
     4201 aaagctttta ataaatcctc tgactgagaa tattctattt ccctcttcaa agatggatta
     4261 gacaattctt ttgttagaga tacccagtgc tttaatcgat taatcgatgt tacatcattt
     4321 ttttctaatg ttttaactaa acctaaaaat ctgtttattc cacgagtctc tgaatataaa
     4381 ttaatattat tccaatcctg taggaatttt tctctatgat gaacatcaaa aatctctgca
     4441 gcaattggtc cgaaaaagag attgtgttta taagtcatcg tatccataac acagccatct
     4501 gaatcaatac aaaatatcca attattcata tttctctcct atttatgttt ttgttttaaa
     4561 agctcgcaat tttctaagac tttatcttta gataatggat aaaggaaaaa taaaattaat
     4621 cctacaatca gaaaacaaat tgatggaatt aatgttgaag cgttatataa cttttctaaa
     4681 acatctcccg tttgtacatt tgctaaagag tcatagccta taatagaaag agtccaacct
     4741 ccgacgcctc cagctagagc ttgacctatt tttctagcaa aagaatatac cgcatatacg
     4801 gtcccatctt ctctttgatc agactttact tcttgatcat caataacatc agtaatattt
     4861 gcccaaataa cagtattaaa cacacccaaa cccatatagc caaccgaact aagaattaag
     4921 aatacataca tattactagc ttttatcaaa aataagatca aatatactat tgctgaaaag
     4981 aacattccta ctgatgccag ttcttttttt ccatattttt tacttaaaaa caaagttaaa
     5041 ggcgaaacaa ctattaaaac caaaatagga ttgataaaat tcattaaaac tattccctgt
     5101 gcatcattat agtaatcagg aaatacataa ttattcatac tcataattaa taattgagca
     5161 aggagtaaga aaatagccgc accaattata gaaagtaatg ccctattttt aactaaccct
     5221 tttaaagatt ttaacaatga aactttgtat tgagattggt caattcttac tcgttcagta
     5281 gttagtttat aacatattaa ataacaaata agtgcacata tcgaaaatat tcccgcaaca
     5341 attgtaaaag ttgatccacc acgaactatt tgagcgcctg aatcatctct tgtataaata
     5401 aataatggtg ttaatacacc aatgaccaaa cttgctaaag cagctccaac atttcggaat
     5461 gtcgatagca ttgttctttc ttctgtaata gaagtaatag ctgatgccat tgatccataa
     5521 ggaatattta tagatgtgta aaaaatactc ccccataata tatacgtaac atacatgtat
     5581 actaccttaa cgctcataga agctgaaatc atagaagttt gatacattaa aaaacttgct
     5641 aaagcgactg gaccactcat tcttaaaatc cacggtttaa acttaccctg cttagttgct
     5701 ttcgatctat caacaatacg ccccatagtt acatcagtaa ctgcatcgat aaatcttgct
     5761 actaaaaata atgttccaac ggcagctccg cttatcccca atacttttgt ataaaagatc
     5821 attaaaaaag aactagcaaa aatgaaagta aaatcgtttc caaaatctcc aaacatatat
     5881 cctattttgt ctcttatacc aaattttctt tccatacaat tctctccctt ctgttgttaa
     5941 gcgttttcat tctgatgaaa gtacgtacta tactctgaag cgtaaagagt cagtatttat
     6001 tttttacatt tcagagtatt atttgttttt tctcctaata attctttatc tcataaaact
     6061 tatctatata tttactagct actcgactta cttcttcaaa atcacctttt tcaacagaag
     6121 ccaataaatt cccacctaca ccaactgcaa caactcctac ttcaaaccaa tcttctaagt
     6181 tctctatact tactccgcca gtaggcatta tatttacgtg tggtaatgga gcttgatagg
     6241 cttttacaat ggatggacca aaagcagaac ctgggaatag tttaatgata tctacgccag
     6301 cagtaagtgc ctcctttatc tcagtaattg tcatacaacc tggtaaataa ggaatttgat
     6361 ataaattaca aatctcagct gtttgtcgat caaaagatgg actaacgata tattctgccc
     6421 cagccataat tgcgatacga gcagtaaacg catctaaaac cgtaccggct ccgattatta
     6481 catctttttg atcagcatat tttttagcaa tttctttgat tatttcttca gcattaggaa
     6541 ctgtaaaagt tagttcaatc cctttaattc cacccgcaat aattgaattt attgctttaa
     6601 tggcctcatc cttatttttc ccacgtacaa ccgcaatgac tccagccgct tctaacttac
     6661 ttaaaatttc tacttttttc atatctatct ccttccttta tggatacata ctttgtctag
     6721 ctgtactaac ttacaaagaa taaatttaag cttttatttc aagtattatt ttctacttcc
     6781 gatttcaaaa aaattaaaat aattatacgc attacgataa caaatattct ccactatctg
     6841 actaagatac atttcatcat ctggaattcg gcctgcttta acccactcgc ttagtaattg
     6901 acataatact cgacgaaaat actcatggcg aggatagctt agaaaacttc gagaatcagt
     6961 caacatacct gtaaaattcg acaacaaact ttgttgtgcc atcgtagtca actgttgacg
     7021 cattccttca gctgtatcat tgaaccacca agcacatccc aattggattt tttgaatcat
     7081 acccccttgg aagctttgca ttgctgttgc tagtggaagc caatcagttg gatttaatga
     7141 atataaaatt gtttttggta agcacccact cttttcagcg gtatcgaaca atgctacaac
     7201 attttgtgtc aaactagctt gatcccccat cgaatcacca ccgacatcta ctccaatttg
     7261 ttcctttaat atacttgaat tatttcttaa agcatttaaa tgcatttgca tcgtccaacc
     7321 atatcttgta tactcagaca tcaaatgtaa ttgaatacct gtaacaaatt gtgcaatttc
     7381 tgataaatgg aaatccgttt ttcctgctaa agcttttatt aacagattgt cgagtatttt
     7441 ttcatctgct atttcataaa aataagtatt caaaccgtga tctgctaatc gacagccaat
     7501 ttgatgaaaa tgatccactc gttggctcaa tgcttgcttc aacccattaa agtctttgat
     7561 tggtatagcc gctgtttgac taagttttaa aatatagttc gaaaagcctg atgatttgat
     7621 actccagacc ttatctggac ggaaagtagg taataccttg aaagaattat cctgtttaga
     7681 aagctcagta tgataccaca agtcatctgt aggatcatcc gttgtacaga tggctttcac
     7741 atccatcatc tccaacaatt ctttcgggcg ataggaatcc gtttgaattt tttcgttagc
     7801 acgtcgccaa atttctggtg cattcttctc agaaattgtc aaatcaatat caaagtaacg
     7861 ttgtaattcc aaatgagacc attcaaaaat aggattacct ggcgccttct ccactgtttt
     7921 tacaaaagct aaaaattttt cataatcatc acctggacca ctgatcaact cttcttctat
     7981 accattagct cgcatcaatc gccatttata atgatctcca gctcctttat catttaacca
     8041 tacctgagtc aaattcttgt attgcttatt ttctagcaaa tatgctgggt ctaaatgaca
     8101 atgatagtcg ataattggtt ggtgttttgc aaatttatga aaaagtcttt tagctatttc
     8161 attctctaat aaaaagttct tatctaaaaa catatttaaa cactcctttc cttccgttag
     8221 tttttagaag agaaaatttt tcctctaaat aaactccctt atataattat ttactcgccc
     8281 ccttttcaag cgcttcccaa ataccattta aataaactgc acctagagct cggtcatata
     8341 atccataacc aggtctaccg gtttctcccc aaatcatccg accatgatct ggacgaatat
     8401 agccattaaa tccattatcg tacattgttt ttaatatctt atacatatct agagaaccat
     8461 attctgattt atgcggcgct tcgtaaaaat cgagtccatt gtcacttaaa tatttgatat
     8521 tacgaacatg tgcaaaagga atccgatccc gttgacaaaa ttcagataga atttcataga
     8581 catcattatc tggtcgttct gcaatacttc cagtacataa tgtaatacca ttagaaggag
     8641 aatccactac attacacaac caatctaaat cgtcacgatt tttaacaata cgaggtaatc
     8701 cgaaaatgga atatggtgga tcatctgggt gaactgccat tttaatccca acttcttcac
     8761 atgtcgggat aattgctttg agaaaatatg caaagttctc tcgtaatttc tcttcatcaa
     8821 cttctttata ggcttctaat aattgcttaa tttgtgataa ccgatgaggt tcccaacccg
     8881 gcaattcaaa attattagta gaattttcta cttctctaat aatttcctcc ggatcagtag
     8941 ggatattttt tgaaatatag gctaacgtag aagacccatc atctaaacga taatccaaat
     9001 ccgattttac ccaatcaaat actggcataa aattataaca gataacctca atcccaaatt
     9061 ctgccaaatt gcgaatcgtc tgtttataat tctcaatgta ttgatctcgt gttggtaaac
     9121 caattttgat atcatcatga acattaacac tctcaataac tttcaaggta agtccattgg
     9181 cttcgacttc atcttttaaa gatttaattc gttctctcgg ccaaacctct ccggctggta
     9241 tatccattaa tgttccaaca attcctttca ttgctgggat ctgacgaatt tcttgtagtg
     9301 agattttttc ttcatttgcc ccataccatc taaaagtcat ttccatagtt actttcctcc
     9361 aacttctgtt atatatttat tgattgtttt acgaactgct tgaggaccta ctagcatttc
     9421 gcaaaagatt ttttcagttt tctttcctac cccaacttga tataaatcta aaccaaagat
     9481 ttctttattt tctaaaattt cctgtaccgc acaatgaatc tttgaaggat cttgttcacc
     9541 taatttcaat actgataatt tatcctgcaa ctctaccaat aacggatcag gacttggcac
     9601 aaaattttcc ccttgatcat cgactgccag taaataacgt aaccaagcag caatagcgat
     9661 aggaataaat gttagatttt ctgcatttcc gtctttttcg acatactttt taattgtttc
     9721 accatagcga atcgctactt tttgagaagt atctgttgca attcgttgtg gtgtatctgg
     9781 aatataggga ttaggcaaac gtttattaat tacttcatca ataaattgtt ttggatcaat
     9841 aatgcctgga ttttcaacta ctggtaaacc ttctaaataa cctattcctt tgattaaacc
     9901 aaccaaatca tcatcctgca tctctgctga aattgattga tatcctagca gacaacctgt
     9961 tactgccaaa gcagtatgaa gtggattcaa gcttgtcgta accttcatga tgtctgcttt
    10021 atcaaccgtc tctctatctg tcataatcac accagccttt tccaatttag gccggccatt
    10081 tggaaaacta tcttcaataa ctaaataatg aactacctca gtattagtga aagcagcaat
    10141 attggtatgt ttttttgtgt gaatgatatc taaatcctcg agccccatca tcaatagatc
    10201 ttcctttaca ttctctgaag gatttggcgt aattcgatca atcatactcc aaggaaaagt
    10261 cactttttct ttatccttta aataagcaat aaatttttca gaaacatatc cttctttttg
    10321 ccaagcttct gcaatcgtaa aaatactatc ttgaaaacgt tgaccattct gtgagaaatt
    10381 atcagtagaa accatcgcaa tcggcaactc tccagcatta aatctgctat ataacaagga
    10441 tgttacaata ctcatcgtat gttgaggatt gctaggtcca gatttgatat cagctttgac
    10501 taccgaaaag aattgccctt tagaatcttt taaactgtaa cctttttcag taatagtaaa
    10561 cgttgtaaac tgtaatttag gatcttcaaa atatttaacc actctttgat agtccgctat
    10621 attcttcgga ttgcaatata aactatcagc cgttactgcc aaaagtcttt ttttgagttc
    10681 accatctcca ctcataacaa tttccaaaat gtcattgtta tagtcatgat aagctttttc
    10741 cactacttct tcatcaaatg tttcacacac aactactcct gtttctaatt cattttgatc
    10801 tgctaagcat tgagcaattt ctgcatgaaa tcctcgataa agatttcctc caccaaaatg
    10861 aagccatact ggatgttgct ggccttcttg cttaacttct ttgacatcaa aacacggcag
    10921 tagaatctgc atttcattga attcttcgat agaattttgc caatttttaa tcgaaaccat
    10981 aattaacctc ctcaaactga tatgttagtt ttgatacatt ttatatttta aaacccattg
    11041 taaccgttgt caatataaaa agggaataaa cttgcttttt tctaccatat cagaatgatt
    11101 gtatttttta ttcagaaaaa taaaatactc cttctttttg gactgatttt atcgaaactt
    11161 attttttctt tttttcatgt attcttttat ttttataact agtgtattac ttcaaaattt
    11221 gatattatat ggcatgaaca gaattaaaat atcagttaaa ataaaagtta aggagcattg
    11281 tgtatgacaa ttaaaactag tctccaaaat caagcttacc aatctattcg ccgacaaatt
    11341 atctatgctg acttagaacc tggaaaaaaa gtatcagaaa aaaaattaga agaaacactt
    11401 tctattggtc gtacaccaat acgtgaagca ctgattcaac tccgacaaca aggtcttgtt
    11461 gatacaattc ctcaaagcgg aaattatatc tcattaattg atttagattc ggccaaaaat
    11521 gctcgatttg tcagagaaca tttggaacga caaattatgt tagagtgttg tgccaaaatg
    11581 aataaccaaa aaaaacggat tttagaaact ataatttcag aacaagaaaa agctgttagt
    11641 caaaaaaatg ctcgtagttt ttttcataat gataatttat tccataaagc ttgttttgaa
    11701 attgcagagc gagaagaagt atggcaatgg ttagaaaata acaatacaca cttagaacga
    11761 tttcgatggc tacgagtaaa aacggaagga ttaaagtggg aaaaaattat ggaacaacat
    11821 tatcaactat ttgaggcatt aacaaaacaa gatccagaag aagctaactt tctaacatcg
    11881 ttgcatcttc atttaatgct taatgagcaa aatattgttt tagctactca tccaaaatat
    11941 ttcaaaaata tataactttt aactcttttt aaattaaatg gttgttctga tcagaaaaat
    12001 atcgcttgtt ttcttattat gtaatatatt tcataaacta ttctgcataa caactattgt
    12061 ttgtactttt tacattggat aactaattat gactgctgat aacttttgat attaaactag
    12121 tatctccaat acaaataaaa aatagatatc ttgttcatca aaattagttc gtggatactt
    12181 accttcattt acatcattca gcggtttccc attttctttt attctattat ttcattttct
    12241 aatagacttg gcttcatttc gcaatacttt ttttcaatag gcatcaaatt ggtgttgttt
    12301 agatatttag ttgtagtttt gtgacatgat aagtatgctc ctttttctct tttccactaa
    12361 tacaacgatc aaagggtgct tttttcaaaa aactagaaaa atcaaaataa gattcgtatt
    12421 ttgattctat atatcctgtt tcaactacat cttatatttc acaatagaat aaatagcctt
    12481 ttgcatagct gcttaactta taagaaattg caatttactt cttcaaagtg aaaacgaata
    12541 tgagtaaaat aaaattattc ttcttacaga taattaaaaa tagcgcgtaa caaaaatcgg
    12601 cattcaatca cataattagg attgatgccg attacttata ttctacgtat agttttagta
    12661 ttcttatttt ttatcaccag taaccaattg agaacagccc taaagcagaa tagtctttta
    12721 tctcaaaacg cttaatttca ggcttaggaa tatgaataat cttttgagta tattctttct
    12781 cttttaatct tactgaagaa ataccactgc ctatcttttt tacaaatatt tcatttggtg
    12841 tacagactaa taaaacaata tttgtaatca atccttgcac taaagaatta aaaggaagac
    12901 tatttaaaac agggtgtgca taactataac atgtgaagta gttattttga actttaactt
    12961 ttaaaaactt taaaaataat cttatttgtt cttctaaaca atcatctata ctcatataat
    13021 cactccacta attaatctcc ccaaaaagac acagcattaa gagatatacc aacacaacag
    13081 tgcaaataac tctacatctt ccttcgcaac cttatttcaa taaaccataa aaattatttt
    13141 ttaatagaaa ctgaaaatta aaactagaaa aagtagtttt accgaatgta agagcatcat
    13201 caccatcaat atagaaatac attcttttcc agttacattt aatttttaaa caaaattctc
    13261 cccaaaaagg aactttttct agtgaaaact tggtaatctc tttgtaatta attcttgtta
    13321 aagaataatt aaacacttca tttttacttg ttattttttc attgactata actagttcat
    13381 ctggtaaaaa aattaaccaa caaacatcga caaacagtaa atgatataat ccatttccac
    13441 ctccaagcat attcaatact ggtgaacatg ccttagaaac acgaatatag ttatggttta
    13501 aatagatatt ttctttaaac attgcttgtt taaaagccat atgctcttgt tctaaacttt
    13561 tcataaactt ttactctaat gcacagctag cagcagctgc tgcaattcct gctacaatat
    13621 ttaccttagc taactcttta gctgcagctt ttcctaattg tttttctgca atttttaata
    13681 ataaatccgc agctttttca aaattaaaag cttttaatgc ttcaataaaa tctccctgcg
    13741 tacctatagc ccaagccact cctcctccag gaagagaatt aatggctaca catccagcat
    13801 atcgtttcca atcaactttc tccattctag gaacaataat atgttcataa aagactcttg
    13861 ctgctcttcc ttgttcgtta tcttgactga ttcgttcacg aatagcacca aaatccttta
    13921 tatggtatac accatgttcg tctaaaaaac caactgtatc aaaataaaaa gttaattcat
    13981 ctactaattt attctttatt tctaaatcaa ctgtattcac tttttctgtg gcatacacac
    14041 ttgttccagt actaacaaaa gtacttaaac ctaataagaa aacacctaaa acaccaaata
    14101 ttttaattat tcttttcata atttctcctc cataaaaaat aagttacttt ttaaatatct
    14161 tgtttatgaa ttcattcccc aaaggccttt taagattgat acagcagtcc gttcacttct
    14221 tttagacttg tcatacaagt cttttaaatg atcccatatc agactagttt gtctatctag
    14281 cttcaatcca ttaaacgcta atgaacggtt cattttttct attactttag ataaagctac
    14341 agaaattttt ataccttcac aacgcgcatc ttggatttca ataatagtat catcaattag
    14401 tttttgaatt tcttgcctat caacatattc aatcttagaa agagaatctt ttaaagctac
    14461 taatgattca acgatttctt ttttctcttc agacattttc ttcctccttc tagtgacccg
    14521 taaagccatg tagagcagag tccattgaac cagtaattac actatctaca acgccattcc
    14581 aatttactct acattttcca tttttcagat ttgtcggtcc atacggatag cctttttgac
    14641 attcaatgtt cggattatac cagataggat tataccattc agccgcagaa gcagattcag
    14701 tcgatacaaa caatcccaca ccaacaaatc caatacacat cacactatag ccaattagtt
    14761 ttttcatctc gtatccatct cctaaaaaat agaatagttc ttatagttat tcataagaca
    14821 tattttttcc gtttttgttt tatcctttct gtcctataac aacttttttc aagtaaagaa
    14881 tgacatagtt tttcttaaaa aaacagtctg tttttttcta tttcttcggt gtataataaa
    14941 tatgagtgtt cattttttat tttaatacgc actccgtaca taataaatct tgatcgcatc
    15001 aatcttttga gcttcttgat ctactttcaa ttgaagtaat acaaattgag taaccttatt
    15061 cattgtttct tctccaaata acaaatattg atccggattc tctatatctt gtgtcacctg
    15121 gtaaagtttc ccagttgttt tcatttgtac atgcgggtct acatcaatac tattgtcttt
    15181 aatacaacga tctgtcatat agtccctcac agcttgattg cgtttataaa catctgtgaa
    15241 attactccat tttctagcaa aatcttttaa gaaatttttt gtcatttcat ctatttgcat
    15301 ttgttgcgct gtctcgtctt ctaatgaacc aatttgactg ttactttcag tactttgaag
    15361 agttccactt gtttctttta ctgcttcgat tgtttcttct gtcgttctca attgtgtttt
    15421 ttgtttttca gaagaaaacc aatgacaacc agtaagacct atcaaacata accaactaaa
    15481 tactaaaatt atcccttttt tcacgtttct agtcactgcc tttcttattt caccttacga
    15541 accagactac gaatttttgt ttgattgtac gtgcgttgat accgagccat aatccgtcct
    15601 tgctcggcat tctgttccac actagagaaa tgttgtccgc catatgaaac atctgttata
    15661 attccggtgt gaccatagat tccaggagac aatgcaccac ctgcttgcca attcaccaca
    15721 tctcctgctt gtaaatcact tggttttggg ttcatgatta cttgccaacc ataggttgtc
    15781 cagttataat cttcccctat taacatcgca aacatgtgtc cagaacccat taactgtggg
    15841 ccacctaatt gagatacata ccatgccgta ccaccataac attccccatt atttacggat
    15901 cgtccaatga tccgatccaa aaccttcgca ttgcctttac tttttcctgc aataagcgaa
    15961 gttttttttg ttggtaggct attccaatgc ttgatcgcaa aagtatcata ttccgttaag
    16021 ttataggacg ttatgatact cattgctcct cgataatacg gactcccttc ggtaatacct
    16081 gctgccgcat agcctccacg acctagaatc aataaacaac ttccaggatc tttattgccc
    16141 acacattgat taaagttgct ttggctcgcc accataaagc cataatagtc ataacaagct
    16201 tttaagttcg gaaacttcat atagaaacca ccttcagaac caccaattcc acgtgcagaa
    16261 ccttgtgggt agggacaacc aggaaaccag gtaatcccaa aaaagttcgt atcgtttctt
    16321 ccagattgtg actgtccata agctgattca tagcctaatt gagaaagaat cacactcggt
    16381 aataactgat actttacagc tgcaccaact gctgcttgcg catgttcatc tgtgattgga
    16441 ccacgtcctt taatctctga aaaaatcggt aatccttcgg tatattcccc attaaaagcg
    16501 acagctagac ctgtagcttc tcctccatgc tcagaaagtt ctcctgaaaa aaagaatggc
    16561 aatagaaaaa agggaagaat aaagaaaaga agacaaccta gacacccttt cttcatttct
    16621 tcctcatcct ttcttcgata atggtttttg gactgttcgt ccttttggta aagtaaaatc
    16681 agttcctggt tttgcgtcca aaatcggcag tgttttagga tccttttcta ttttttccga
    16741 ttgctctgtt ggcttatcta aagaagaaaa tgcaggttgt aacgattttg gtgcggaaat
    16801 cgacggttta ctttcagacg aacgttgccc cggttgttgc acagaacgat ttcctgatag
    16861 agtggctacc gacgaattag gaacagagta ttggttcaat aactgctgca ttgctggata
    16921 actcccattt ttcgcctgat ttttcacttg gcgaatctgt tgtgaaagag aaccagcagc
    16981 tgttaattgt ggcacgggta cttttccaga aggcaacgga atcgattgac caacaaaagc
    17041 gataccttta tttaacaatg gttttctttg attattccct tgttgcttac tcaattgtgc
    17101 caatgattgt acctgttgtt ttggtcttac tgaattcatg gacgaatttg gaatcaaacc
    17161 atttgaaaga ccagaattcc actctgtttt cgatttggta gaagacattt caggctctac
    17221 cccatgataa cgaacatgac gaccactata aggcaaggct tgatttgaac gcccacctag
    17281 ttcaaaggga ttaaccactt gcctcccttt taccgattct tgtggtgtac gtttttgttg
    17341 aaacttttga aatccatgta tcgctgcaga agcgagtgga ttcgttgctc gatacacatt
    17401 ccctgctaat cctgcacctt tgattgccaa attttgtcca gcagttgata gttgaccagg
    17461 cagttgtttc atttgagaca aagaatgact tgctatgttt ttcgtgttgt ttatagtttg
    17521 atctgcagct cctaacccta gtttctttaa taaatctttc cgttttacaa tgagtaaaat
    17581 tgataggagt gctttgacaa acatatttcc taaaaaacca aaaacagtat tcattggaat
    17641 taaatcgtct actatgttat agatcaaaaa tgccaccaaa ataatcacac tgtaaaaaga
    17701 tttttgaaac atcaagccca aaaaggactt taaggtattc atgagcatat aatcaaatac
    17761 tggaacaaaa gaagcgagta atgaaaaagg aatcacaagt aataaacaca acaccaacat
    17821 ttgaaagata aaacgaatca tgccaatcac tacaagtggt aatccaatac ttagattaag
    17881 caatggcacc atagaaccaa cgactgcttt ataaacagct ttagttggtt gaatatagac
    17941 acgataagcc ttctttttcc ctctatcttc tcctgcagca gtggtcactt ttgtctcaat
    18001 atcagataat tcttcacttg acgcttcctt ggctagaaat tcttgtggtt caatctctgc
    18061 ttgatacaat tcttctaaat ttgttgtccc ataatttaac aatatatacg gatcaacaac
    18121 agccgcttta taataaagtt ggcgcacttt ttgaatggct tccttttgtg tcgttggcac
    18181 taaaacacca ttgacatctt ttgtcgccaa ttcatctaga ctatttgtag cagaaaaaat
    18241 aaagccttct acctccacgg acaaatcatt taaccctttg gtaaaagctt ctcctttact
    18301 aggtacgtta ctaccatagc caaaccaaac cattgaaaaa atgacaacga agagaaatcg
    18361 aataaacacc atttttgcgt aatgtttccc tctaaatata cgaagagtaa atagatataa
    18421 cacatacaag acgacaatcc ccgcaccaat aggatcgtat atttttgaat aaatagtatc
    18481 cgaaaaagtg aaaaactggt gaattaactc gttcattgca gctccttcat agagcttctc
    18541 aatgacaaaa tcaaggcctt gccaaatcaa tttacttaaa ctaaagaaca tattcgtcat
    18601 catttttaga aaatctgtgc catttgcccc aaataacatt ttgcctaatg aaaattcact
    18661 atcagataaa taagaagaaa aggtatcaat atgaaatttc ccaatctcat agatcgattg
    18721 atccacctgc ttttctgccg catacaccaa tccaccaaca aaaagcagtc cgaatccaat
    18781 cgttaaaaaa aacaaaacgc accatttctt ttccttatgc tttatataag ccattgtttc
    18841 acctcatttc tattgcatga ttaacgtatc ctgttcttct acttgagagt gacttgtttt
    18901 ttccgttgtt tgtaatgcag cggtcatttc ttcaaacaaa ctgtgtacat tgagttttcc
    18961 tacacgtcca tagggatcta acatccaaca ctgtccttta atcatgtggc tcatttcagc
    19021 taagttcgct tcatttactt ccaaacgtaa atgattcaaa atcttttctc gctcactgtc
    19081 ttcatcaaaa gcaaagacga caccaaaatt ccctttttct ttatctgaac ctaagtcacc
    19141 aataaattgt gtatctaaca ctaattgatt catctgggaa cgccccacac gtgccattgc
    19201 tgcaataata ctacgtcctg cgttcgaatt attaaatacc catgcttctg taaaataaat
    19261 cgctgttttc ttactactat ctcgttttcc aaacatttca caaaaacgac ctaaactaat
    19321 catcaaacac aaagactttc gatccgcatc tgtatatagt tctggtcgga cattgtcatt
    19381 tggtaatttc aaatcttgga tctctaaaat cgttgttttg gcattaaagt ctaatcccgg
    19441 gttttctcca taagaaaatc ctaaacgtaa aatcgaatct tctaccgtca agcgtaaaaa
    19501 ctcaccatat tctcgaacct ctttttgttc catttgttct aaatagtcga ccacatgcat
    19561 caagccaact tgttgtccgg cttctttttg aactaaaact tcttggattc cctttaatac
    19621 cgcccctttt aaaaggtgat tggtacctaa cggacttaac tcatcaatca tatcctgggc
    19681 tacttgtttc gcatctactc gggataaaca aacaattgga tctaatacac cataattacg
    19741 gggatttgta gcatctaacg tgacataact cattttttgc aaatgttcta caaacaaagg
    19801 gtaatgtttc tgataatacg ggtcttgaat gagtttcgta aaccatttgc gcttttctcc
    19861 tttgggatcc acaaataaac tttgtacatt cataaacgaa ctatacaaaa agagcaagcc
    19921 aactaaaaag gtcttccctt taccggtttt tccagttact gctacatgag gcgcatcaaa
    19981 caacgcacct ttgattcctt ccgcaatggc tagaagattc aaaaagacaa aacgatttga
    20041 cgcataaatt aattcttcta atggtgtttt ttctcctcga gaaccaccat cccctaatgt
    20101 atcttgccga ccaatatacc agccggattc catacctaat tctgatactg ctgaaaacaa
    20161 ccattcgcca actgcttcta cagttgttcg gtgcttccaa tgtaactgcc gtcccaatgg
    20221 ttgccctaat aaacatttat agaacaattc tcgttgatca ttttttgcac ggtggatatg
    20281 gataatcgta tgaagttgtt gaataaccgt tgcactattt tgacgaactt cttcaaccgt
    20341 ttgaccgtat acaataaaac atgcgatcca atcgacgaca ggttgcccat tatctactcg
    20401 ttgctttaaa tgttttacta atcctcgaac agttaataag cgttcactag gttcttcatc
    20461 attttgaacc aattccttat gctctgtttt gaaccgttcc gatagccaat tcagttttaa
    20521 accaatacct ccctgtcctt tcaagcgtgg atagtgaatt ttcattcgca attctgctgg
    20581 gaaagaaaga tcctgagcaa attgaaatag atcacaattt ctcatatttt caggtgtttc
    20641 cccaataggc aacaatgcta aataactact ccctaactca tcttctaatt gaagaaggcc
    20701 tttatgttcc ttcggtatac gtacatacgt tccttgattg tctttcagta cttcccaatt
    20761 attttgttct tctactttgt gagccattcc tcgaatatat tgcagacgat tgagatacaa
    20821 cagctctttt tttactaagg gacgaataga aaagctataa agtaattgtg tcacttcttt
    20881 tacataagga tcactttcag aaaacaaagt ttccaaatca tctacttcta atccaaataa
    20941 gcgtgctaaa cttcctgcta aatctaacca gattagattt attacatcat gaaaactctc
    21001 caccgttgct ttttctttga ctttgattcc taaaataaat gaaggacgat agacttgacc
    21061 ttctcgttcc aacaatgtca ctgtttcttt tgcataatac gcaccaattg cttgactatc
    21121 gggagaaaat ccctttccta atgtgtcaaa cttcgtccgt aaattcattg ttcgtggata
    21181 catcaccaac tccacatctt cataatctgc taaaagcaac agtaaacgag ccatcatttc
    21241 ttttatttcc tgtgtctttt ttggtgtgtt ttttgaaatt gttcgtggaa aaacatgata
    21301 gtaggcccag atacaatcct ctccatttaa caaacaattt ttcttgaatg cttttactgg
    21361 ttcttttaca agcaccaatg aatttcacct cccaataaaa aaggaacaac cagttgattg
    21421 tccctttttc taatcctcta aataaacata cttattcaca aaaagtaatt cttccaatgc
    21481 attgtttgat accttgtccc agttataata cacacaatca ccattcatca tacgaatcat
    21541 ataacaaaac caatcccata aataaaaatg cacttttttt ccatcaggtt ttagttttgc
    21601 cagtgctaac acacataaat aggggagtcc tatataaaaa acaactcctg ctggtggaaa
    21661 aaaagaaatg acccaaccaa taaatgaacg taaaaataaa tattcaaaga ttacgaccaa
    21721 caaaaaagtt gctgcattga ctaatgcaac tggaaatgga agacgccaat tgcttgtaat
    21781 ctgttgtacg cgataaggtt ccgcataaac aatcgaataa tcataaggtt cccaatccac
    21841 ttcactatcc tcctaatcca taaataattt ttcaatgatt ttaccaaacg catcaaatac
    21901 acgtgttgga tcatttaaga agaaataaac tgcggcacct gctgcgatag atattaatgc
    21961 atttcctact tttgatttca taaaatttcg caatcccaga taaacaacca ccattattaa
    22021 aaaatagcca ccttgcgtta atataaaatc tacaattccc ttcagtgtca tttccgttct
    22081 tctcctttcc tatccaacca attatgtgtt aatttttcaa cataatactg atttgttttt
    22141 ttcgtaatga caatcgtcat ttgttctttt tgtaaaagtg acgtatcacc atctcgaaag
    22201 acgaccgttg cttttacaat aaaaggtttt ttcgtttctt tataacgcaa tactttcatc
    22261 tctacttgat caactttagc tgtttgattg agtccttcag gttctttcat catgtatgac
    22321 atatcttttg cattgttttt cgcatactta tctaagaaat cttccacaaa tccttcaaca
    22381 tctttcgctt ccgacgcatc aaccgtttcg tataatgtgt catcataagt aatcattcgt
    22441 tgtttttctg tattctttgg tagtgccgta aaataaggat aagcaataat acgacagccc
    22501 ttgtttgaaa tagaaaaggg aagattgagt acttgtgtga ttgtcacact tgtttctttc
    22561 ttactctctg gattcatcga gcgaatatca taagttactt tataactagc agttggaatg
    22621 gtttcaattt cttttaatcc aataaacgaa gcagcagtta attttcgaac acctgaaaca
    22681 cttcctggtt cttgaataaa gttttgtgca taaaactctt tttttaatgt ttcttgacgt
    22741 tgttgttctt gttcatgatt atcgaatgta ttcatataca aagcaacaaa acgattcata
    22801 aaggattcaa tttcaggtgt gtacgttaat tctgaccgac tttgtacttc tttcttttgt
    22861 aattgttgga tttcatcttt caaaaacgac acttgaacct tcgttttcat ggattgaaaa
    22921 aatgccaagc cccctgaaaa acaaagaaat aaaaccaaac accaaatcca tttcactgct
    22981 ttttttgaat ggattttctt ttgcttcggt actttctctg tataaacttt ttctttccgc
    23041 cgtagttgtc gttttttcat aagctactcc ttctagtatg tttttactag tacgtacgat
    23101 tgacaacgat aagcttgttt ttctgaaaaa tagtccattc aattaccctc attttctatt
    23161 ccttttctct ttcttttata tccaacaaat ccagcaatac ttgtcaacac tcctcctaga
    23221 acagaaagtg caacatgtat ttcttcacca gtctttggta agacttttgt ttttccatct
    23281 gctgttttga tttcaatatt gccacttgga agtttcttta caggaatcga caaatccttt
    23341 accaaattcc cttcactatc ttggattaat ggaataccat cttttacacc aacaattgtt
    23401 tgatcacctg ctacttttaa tggggtggtt ggtgttggaa tatgggtcaa ttcctctaca
    23461 ttactagtga caatcggtac actcattcct tgagaagtat tcgtcgtaat ttcaccgctt
    23521 ggggttaccg atactgtgac ttcttttgct ggaacaatag gtttattttc ttttggaatt
    23581 gcttctgatt tttcaacagg ttgtttcggt tcttcttttt gttcttcact ggtagacgac
    23641 atcgttggct gttccgtact tgacgaatca gtattctttt cagaagaact ggttgttcct
    23701 gattgttccg gagttgtgac tatacttgaa tccgttgttg ttgaactatg cacaccttct
    23761 tcttcgatcg aatcagtcgg taaagttgat tcaggaaact ctggttctgg tgtcactggc
    23821 tctttttcag gtttctctga ctgcaattgt tcaggttctg ctggtttttc cgttgttcct
    23881 ccatttagct ctgtctcaac atctggtaat ccttcccctg gcaatgtttc tacaatttct
    23941 gacttaggtg tttctacagt ttcatctgca taaaccaatg aactccctaa ttgaaacgaa
    24001 aataatgaaa ttcctagtgt tgttacgact gctaatgttg tttttttcat gattattcct
    24061 ccaatttttt atgcaattcg tgttttgttc atagacgccg cactaatatt atctgaacga
    24121 taaaagaact cccgtccgtt ttcttctaac ccaacatatg gattattttt gaataattga
    24181 atcaatagtt cactggtgtt gtattttgga tagtttgcaa aaaaataata ctgtgcttgt
    24241 cgcccatccg aaaattcaac aagtaattca taacgatacc gcttcaatcc ttttcgttgt
    24301 tttaaaggat ctctatacca acttgtccat cgttccattc gtggatagag atagacacga
    24361 tacacataga acaatgccat accacatacc accaaaaata ataatcgcca cataaaaatt
    24421 cccccttttc ttctaccttt cttgaatcat cgaacattca taccctctat agagatttga
    24481 aaaatccatc atcaattgtt catataaacg ttgttgcaaa tagcgattgt ttcgaataca
    24541 taattcttca tagccatcaa tcacttgatc ttctttttca tagcatcttg tagctctatg
    24601 acgctcagct tgcatgacat aaccaaactc tgcaccatat cgttctactg ttaataactg
    24661 ttgactacgc aataatgcaa tataacaatc acaaaaatgt tcaaccgaca tttgattttt
    24721 ctcacgtttg tgaaataggt attctttaga aagcacatta ttcgttaaac aatctgataa
    24781 atagcgataa tctggcggtc ggttattttc atctgtgaat ttttcccata ataaactgat
    24841 ttttattcgt tcagcggcta gatagtcttg ttcgcgtgga taaaattcat caatcgcaca
    24901 aagtttttta tcacctgaat aaacaaatcc atataatgcg aaacgaactc ttccttttca
    24961 attggctctc ctaaaaattc cagtgacata ccatcttctc cttcctaaaa aaaataaaaa
    25021 agcagaaacc gatttggctt ctacttttct ttccttttat agctcttttt tacggaagaa
    25081 tccgcaaatg acattttttc aaaaaatcaa tcggcacaag ttcacgaaaa cttggagcaa
    25141 ttttggatag aatcacttcc ccatcttgta aaatacaatc ataaaaatca tcagttactt
    25201 gatacttacg cttcttacaa taattgataa aatagtagcg atcattttct atttccattc
    25261 gttgttctac taagcgcata cgaacatcat catagaaata agccacaaat cgcccttcaa
    25321 gttcatgttt ttcaatggga tactcttctg gtaacaatct attttttcgt cgtttataca
    25381 ttgctggata ccttagaaga agttcatcta aacgtctgtc agtatatggt aactgacgaa
    25441 tcaacgattc taatcgttct ttatacagat agaataattg gcgcaagcaa tagtgtaccg
    25501 cctctggatt ttctaatact gttggatcac gtgaccaata cgcaccaaca taaatcataa
    25561 tttttttcat ttagacaatc acaccagcaa tactaatgac cgatccacaa taggtagaac
    25621 aagtggcata gcgaccaatt tcctctaatt ctatctcaat ctcttctaaa gaaagatatg
    25681 tctgttcttc tgttggctgg cgactcattt cccaagtgac agtcaccaat gaaatttctt
    25741 ccataagagg tttttcgctg ctaagtgctg ccacacagtt ttcatatatc ttcattttat
    25801 cgcagccttt tttaaccgct aaaatcgcag gtattggcaa tgaaaatcga taaaagcgaa
    25861 tgaattctag ccttcctttc cttcggctaa attccaactc ttcgagttct tgttcgtatc
    25921 ccttacgaac ttctaataag cgtgcaattt cttgccagtt ctctttttcc tgataacgtc
    25981 cagcttttac cgcttgttca atataaggaa ctcgaaaacg aaaataagac aaatcaacca
    26041 tcttttcatt taatgcttcg attgcagcct ttaaatccaa caggcgcagc cattctgttg
    26101 tcgtatatgc attcatgcaa ttttccctcc tgacgttccc atccattcta gatagttttg
    26161 ttgctcgtca tttagttcgg cattatcaat cccttcttgt acaaagtctc ttcctaaaat
    26221 acgatctgct tcttgtaaca actttaatgt cggagctact tgtttcaaaa accagcgttc
    26281 tgaacgatct aatgagaatt tttctggctt tactgtaaaa tccaattctt ccatcgaacg
    26341 aaataatttt gcccatcgtt tatcaacaat ccgtgcatca ctttccagtc tatcccaaac
    26401 aataagtttt gcattaatta aaccaacgcc aatctctcct aaacgtttcg gtgaatcacc
    26461 taaaataaat gcttctactg cttgtgttgc tttctcattg gctaaccgta actcatatcg
    26521 attataaata ccatatttcg ttaatgcttc taattctgaa attttttctc ggttcgcaat
    26581 ttcaaaacgt ttctcataaa aattaaaaaa gagaggactc tttcgcgatc caaaatacaa
    26641 agataatcct tcacttcgaa aatagccatc atctaaaaaa caaccgccaa tattctggaa
    26701 tctcttaaag tccatttcta ataaacctgc tttttgtttt tctagtaatt caaagagatc
    26761 gaaatgttct tccgtttcca atccccaaag ttcatctaac gcaatatcga atcggctaca
    26821 ttgcacacgt gtataaccag cttccaactt tccttgattt ttccaaatgg aatatagcca
    26881 tgttcgccaa tctgtttgtt gttcacgcat gacttcctca tattcccggc aaccttgtcc
    26941 tgaaaattgc acaagtacat tttctgttac agttcgtttt gaatgccaga acacccagat
    27001 attcccacga acagaaagac agtcaaaaag atacaagccc gttggacgtg tttcaaattc
    27061 tgtcagatcc attcgtaata cacgttgaat cactgttttg tagtctgtcc caaaaaagga
    27121 aagacgcaaa taatcaatca ttgctcgaag atctattccg cttttcatat acaatcgaaa
    27181 gcgttccttg attcgttgtt caaaatcctc tgggattttt cttttccctt gttccatctt
    27241 tctaatgtaa tcttttgagc agcccatcaa ttgcgcaaat cttgcttgtg atagcggctt
    27301 tttatctcca cttatattat gaattgcact taacttcttt ctgtagcttt ttagctcaat
    27361 cggtgtcatt ttctcgctcc ttttataaaa taacaaaatc gatttttttt aaccctttag
    27421 aaacaccgat atatcaacta gtccccctcg ttttttggga aaagtagtac aactgctgac
    27481 cttagataat cacttgatac aaaagggttt ataaaacttt tttctatgtg ttatgttatt
    27541 tctgcccccc atattatata tcggggggct ttacacaaag aggccgtgcg caagcgcacg
    27601 acctctttgt gagctgcaca cgctgctagc gagtgcgcat tataggccca tgcttcgcag
    27661 ccttaatgcg caccgccagg cagagtgcgg ctaccagact acgtctagag ccctcccgat
    27721 agataataac tacaaaatta aatgatctaa ttttgttatt tcactatatt tttcaggaga
    27781 aaaaacgcga taaattgaca aagttaaatc ctcttctaaa tctactcgat aatgatacaa
    27841 tcgagtcaat cgttctatat cttttttcgt tagcaatact tgattaataa cttttcctgt
    27901 aaaaacacgt tctttgcgca aaaagcggta atcagttact tgttcaatac gattcaacca
    27961 atcataaaac gttctggaag aaagagaagg attaatcatt cgtattgctg tagctactct
    28021 ataataacaa ctctctgcca tttaacttca cctccttagt tgattacaaa atatgcgcat
    28081 cagaaaaact tgttcctttt ggatcttttg ctaaatacat ttggcgctct agttgttctt
    28141 gtaacaactg attatcatct cccatcgttt gatcttgact atcctctttt actgtaaaat
    28201 catagacaat catttcagta aatttctcaa attctttgat aaagtgataa tctttgtaaa
    28261 caaatggcgc aaaaaactct acaggtacat caaatcctgg aaaagcatat ccacgaccag
    28321 gttgatcttt gttgtaaaat ggcttatttt taaaatcttc tccaaaagtc attaaataac
    28381 caatgggaga caatcgacca acagaaatac ggaatgttaa attatcccga acaccacttg
    28441 gaaaatattc tgcatctggt cgttgcattg ctaaaataat gtacatccct gcttgacgac
    28501 cctttaatac taatggctga atcaatttat cgactctatt tctcgcttcc atatccaacg
    28561 aaccataaaa agctgcccat tcatcaaaaa agataaaatg cggtggtaac ccataataag
    28621 cataattttt ccctggttca tatttaggat ctttcatggt tttcatatag gcataccgtt
    28681 gattcatttc ttttaatgca tttcgtaaac aaataatcat ttcttcattt attttgtaaa
    28741 acacatgtcc tttgaataca tcgacgtcac ctaatgctgc taaatctgcc gcttttccat
    28801 cgcaaatatc tactgtccca acacttaaac aagcttgaat taaagaatac aataaatagg
    28861 ttttcccacc accgattgca ccagcaatca acatatttgg tgattgatca tatacccaat
    28921 ccaaagtttt cattaaatga atacgtccat tttctgctat acattgatca ataggaatcc
    28981 gaccagaatc tgaaaggaat acataattga ttcgggcttt ttctcgttct tcacgaatta
    29041 aatcacgcaa aaatacttca gataacaaat gacccatttt taaaaatcgt tcgtgaaaac
    29101 gcccaccatc taaaggaaaa gaaaccacta attcttgttt cttttgataa aaataaactt
    29161 ttggtaatct cattttttcc cttgtttgtt tctcactaac ttgtttcttt gtataaacaa
    29221 accgattgtc aattaacatt cgaacaagta actgacgcca ataaacagag gcaaaatatc
    29281 cttcttttag tcgcgaccaa tgcatcaacc ctaaaattaa cccactaacc agcaacaaat
    29341 acaacacaat acttattcca ggaataagat acatttgcca attcgaaaac gtacttaaac
    29401 gtggataaaa tactttccaa ctaaaccaac ctaacagaat caaaaatgga aacaaaaaga
    29461 aaccataata gcaaaaccat aaataacgat tgaaatagcg aattcgccga ccacgatact
    29521 tgaaaaaatg agctttcaat tgtcacattc ctcctttctg tttttacttt tcctgtgcta
    29581 aaaacttttg acaaaaagca actaatacag gaatatcaca tttagtaaaa aaacataatc
    29641 gaaaaagatt taatcgattt ttataagggt gtgcacacat attacatatt cgtactatac
    29701 gaatccaaat cacttcctga tgatcttgtt tccactggta cggtaatgaa aaacgaacaa
    29761 gatcacgtaa aaacatacac tgcccaataa ccgcacaaat atatgcattt gtttgtacaa
    29821 aaatcgttgg attgtctcca aaatactcaa tcaattgttg gatattttga taacaagcac
    29881 aatctctcaa agtatttgat tggttttatc tgaattcaaa atctcccctt ccttctatct
    29941 tagataaaaa gaataggaag actccttcct attctttcaa aaaaacctta ttttttctct
    30001 ttttccttag aatcaacact tggttgtttt tgatctaaac ctttttcttg tgattgtttc
    30061 atattaggat tatttgttgg agcttctact ttctctaatc ctttcgcata aatcactaaa
    30121 tctgaatatt cacgattgcc atcttgtgct ggaatatata ccacatgata ttctggatca
    30181 attaatttca caggatcacg tcgtttaaaa ccagtcaaat ctacatctgc tggaaattta
    30241 actcgtcgtg caatctttgg ttcatctgga tttccaaaaa ctaattgtgc tcgctgtgat
    30301 cgaatttctc ccgtataatc gcctgtttca ttatcaaata cacgatcaga ctcttctaat
    30361 gcgccaaagc gtacggctcc taaacaagtt tcatctaatg cttctgtaat gtcataatta
    30421 tttggaaatt ttaatcccat cttacaaaac ctccccaatt tttacaccag ttaaaaagac
    30481 accaacatct tgatagccag ttgttcgacg ctctgtttgt cccatatcat ttgtttcacg
    30541 actattcact cgttctttac gaaaaacaaa ataagcttct tcaaatgaaa tacgcacctc
    30601 tttttctggt aatcgtaatc ttttatcctc ttcagctgtg agataaacaa aaaaagcagg
    30661 ctgttcttct cgcgaacact ctgcttgtaa ttgaacatat cgcccagaca caccattaat
    30721 ttcctttggt ggattaggtt cccgtgaacc caataaaaat acttgaccta aaaactcttt
    30781 cttcatacgc aatgcttttt ctgccataaa gtttttcctc ctcttaaaaa ccaaaataaa
    30841 ttctaaatat ttctacatta caatcaaaca agcggttaaa aatcctaacc gcttgttcct
    30901 catgactaat ctttggcttt acgtacttta taaagtgtta tacctaaagc aatgactaat
    30961 actactaatc caagaaccat aagtatacta cttgtttgtt cacctgtttt tggcaacatt
    31021 ggtacaacag tagctggttt aacccgttgt tctggtttca ctggttgcgg tggttttggc
    31081 gcttccacaa tttgaaccgt ttgatctttg tttgttagat ctacattgtg tttcgccact
    31141 tcttcaaacg tttctttctc atcttccgtt ttcgcttgga gccattccgc aaaactcaag
    31201 ccttttcctg caagattgcg accgtccact aagaattcaa ataagaattc catcatttct
    31261 ttatcggccg caaagacttt ttccgcatga gtgccttcaa atggtgtgcc gtctggtgtt
    31321 aagtagcctt gaacaattaa gcgatagatt tgaccaatcg caagaccgtc tactttgacc
    31381 cattcaagca cagtttgatt tggcaaggcc aagatctctt ttccgccatt tttattcgtc
    31441 gctttcgtcg taatggttgg attactgatt ttgatggttt ggtcattgtc ggtaatatct
    31501 gcgtgagaag ccaataatac gccttctcta aacagatctt caaagaccac gacttctttg
    31561 cctcgcaacg cacgtgcgtc tagaataaag tctagattga ctgtaccatc cgttgtttct
    31621 gccacaaacg ttaaggtact tgtgacttct tttccatcta ctagcactgg tttttccgtt
    31681 actttgtcca ttagaacacc gttgactgtg tatgtttttc caacgaccaa atcgtggtat
    31741 ttcacagcgt ctgtaatggt gactttcgtt aatggattaa cctctttcaa gccagtgggt
    31801 ttatgtgtag ccgttgtacg tacacttggg ttagttactc gtaccgtttg ccctttgtcg
    31861 ttgatgtctt tatggtttgc tagttcttca tcatttctaa aaagactttc aaacacgacg
    31921 atttcacgac catttatacc tgtcaaatca aaggtaaatg gtacgatcac cgtttcattt
    31981 tctgctttgg caataaagcc aacactcgtt tctaccgtat ttccatgcac aagcaagggt
    32041 tcaccggttg ctttatccat taagatccct ttgaccaagt atgcttttcc tggaagaatc
    32101 tcggtaattt caacggtatc gtttaaggta aacaatcctg ttgggtctgc gactgtcaat
    32161 ccgtcttcaa agacagccgt ggtatgaacg gttggattat tcactttcac actttgggct
    32221 tcatcgttga tatccgcatg tgctgcaact tcaatgccat cacggaaaag tttttcaaaa
    32281 acaacgatcg tttgaccttt cagaacacta gcattaacca caaattcaag gtcttttgtg
    32341 ccattgactt tgtctgcaat aaaggccaag gtacttgtga tttctttgcc atcttgtaaa
    32401 attggcgcat tcgttgcctt gttcatcaat actcctgaaa tggtgtattc ttttccgaca
    32461 aacaagttgg tataagaaac actgtcttta atgacgatcg tttctaatgg atcaaccact
    32521 ttttcaccat cggctttatt tgacgcttgt gtttctagtt ttggtggaat caagtaatta
    32581 cgtacagaaa ccactaacgt ttccccatca tctggcgcat tttgtttgaa tgcaaccgtg
    32641 aatgcttgtt tatctgtatt tggttggaaa ctttctaatg gggcattttc caagaagtaa
    32701 tagtcatcgg caactagacc tttcactaaa atttccccgt tttgatcagt ggtatattca
    32761 ccaattgctt gatttttacc gttatacaag gtaaagttca cacctgcgag cggcgttttc
    32821 ttcttgttcc attcttcata cttgaataat tttacacttt tccgtgcttg acggttcgca
    32881 aaatggatca ctttgatcgg ttctgtatca ttggtaatcg agaacactaa tgggttttga
    32941 ttcaaaacgt aatttacggg ggctttgatt tcttccaaac gatagcgatc ttttccataa
    33001 gtaagtgtac cgttagttag gaaaaagcct ttttcattgg ttttaaagat atccgttgtt
    33061 tctgtatcat ttggaatctc cattgtgaca taacgattgg ctaaagtatc aaaaatcttg
    33121 aattctgtat tagcaagtgg aatcactttt cctgtttctt gatccacttt caccacttta
    33181 agacgttgtt caattacttt atcttctaaa acataatgga aagtttgccc attttcagaa
    33241 attgtgactt caagtggttc aaatagttta tacccttctt ttcctttggt ttcagaaaga
    33301 atgtacgtat caaagacaac ggtattttta ccaaaagtag cataaccatc attgtctgtc
    33361 acaacggttt tcaccacttt gccagttgtt tttgacgtca aagtgatttc aatgccagct
    33421 aattttggtt tgttatctgg attcgttgaa tcgactaaat ctttattagc aactttcact
    33481 aaatcaatgg ttcctttgat cacttgatct tttgcggtcg cattgacagt ttttccaggt
    33541 tcaacaacaa tttcaattgc ttcatcagaa agcacataac cttttggcgc tttgatttct
    33601 tttaaaacat atttgccagg atcaagatct ttaatttctg cctgtacttt gccgttaatt
    33661 tttttcaagg taatttcttt aatctttttc ccatctgctt gatacaaacc atagaccgca
    33721 ttttctaaag aagctgcacc ttgtgctttc acaccagttt ccgcatcttc tttaaacaca
    33781 cgaatgtttc cttgttttag aatttgtaaa ttaataacag acgcattagg ttctgttgga
    33841 tgtaacacac caattgtttg agctcctgga cgacgatagg ctaatggcgc accttgaact
    33901 ttaattttat tcaattgaat ttttgcttta tccggtgcct cttttgtgac tgtaatccat
    33961 acttcattgc cgtgaataga tacattgaca ccttttggtt tttgaatgtc tttcacatat
    34021 ttcaaaaaat catttttatt ctttactttc aacgtttcgc caatttttac cgtatgtgtt
    34081 tgattattaa aagttgcttt ttcaccaaag ttattaatca aacgttgaat ttctgctttt
    34141 cgacgatcat aatcaacacg gatatttttg attgggattt ttgtgcccaa agcttcccaa
    34201 atcataaatt gtgccgcaat gtattgttca tcggatttgt ctttatttgt gacatatcct
    34261 aaataagaga tcaagcttgt ttttttccgt acatttgaat ttttcaaata gtttttcaaa
    34321 gcagtagctg tataaacttg accttctgcg gctgagacac caggctcaat acaaaacgcc
    34381 acttgtccat taatctcaaa acgaaccata taatttaaag tgaagtttag attgttcgga
    34441 ctagaagtaa caccaatatt ttcgccccaa tcaattttcc gaccacctaa aacagctgca
    34501 ttggttgcac ggcgaacacg aaccgaacga ttggttggta ctgttactaa cagtgtaggt
    34561 gtacctgttt ttggaatgtt ctgctctttt ttcttttgat tttcaatact agccgtagat
    34621 tctttgtttg gtttcgttgg ttgattttga ctagctgcgg ttgaagagat tgttttttca
    34681 acgttttcct cattttttcc ttctttgtca gcagtagccg ctggttgttc cactgtttct
    34741 tctattcgtt tcttttcttc cgcttgtttc tcagaagact ctggctgcac agaaggcgtt
    34801 gcttcggcag ctttttctcc ttctagagtg gacgtttctg aagcaggggt tgtaattgag
    34861 gcagtttctt caactggttg tgaagtacca ggactattcc ctgggtgttg tgtttcccca
    34921 atcgtctgat cgagagtcgt tttggaattg accgttggcg cttctgtcac aggggcctgt
    34981 tcaacagtgg cttcaccagc tgggaccccg gcttctgctg gttggacttc tgtcacttca
    35041 gaagcgactt gggcaatggc ttccatcgta ggttgtatag ccgtcgtttc ttctgcatgt
    35101 gcttgagcag ttcccaccaa gaaacagaat tgagaaaaag ccaccaatgc gagtgctttc
    35161 aaccaattca ttttcgtttt tgcatacaca agaattcctc cttttcctga gaattttata
    35221 taataaaaca aaacgccgtt accaaaagtg ataacgacgt gaactgtctc ttctattttt
    35281 atctggcgct cttatctgaa cacatttcat caatgatttt accctcccac tataattaca
    35341 gaacaaaaaa agaacctgct tcgtggaaat caactgtcca tttcccaatt ggaataccag
    35401 cttttttggc aacttcacca tcaccaaatc tcatgccata ccaattacta ccttcatcct
    35461 taatttcttt ccaaccatat gacatagctt catctaatga atcaaaaatc atgacactat
    35521 tgccaattgt tacaggatct ggcttaagat cttctggaag agaaatatca atcataccat
    35581 aatgatccga ttcctcagta ttattgtcat tactagctac tggtttggtt gttctactgg
    35641 cttattttga tacaacaagt actcttattc ttcttttact tctccgtttt caatttccca
    35701 ttcaaaccac tccgcaaagt cttgcttaaa accatctact cgatacctat tttgtaaata
    35761 ggcatcttct gacgtcgtag gatctaaata ggtaggttta ttctctggac gagcatagac
    35821 acctacccat gtttcgtaat ctccttcaaa ataactatct accaaatagc ctttttttct
    35881 aagaatctcc cgaacttgat ttttaattat tgatacttgt tcaaaaccat cactcattct
    35941 atcacctcaa ataactctag attctcataa atattcccga caatttcaat cctatcaatg
    36001 atctctaaca aatcgctact tatgcttccc cattcgtata agaatttccc ttccgcaaaa
    36061 taaattttcc ataaacttct tcatgatcgt cccaaccaat atcactctcg aaaaattcca
    36121 cgttgttctt atcttttagc tcagtaaatg ccataagttc aacatgtttt ccaacctcga
    36181 aacaatcaca ccacccatca accgttacac gtgtgccttc aatacctaag aaactttgtc
    36241 ctcgtgtatt aaacactaaa actttccgca ttttttgctc attaattaat cacgcgcgaa
    36301 atctaggaat cacaacttat tcgcttcttc tttataaata aaaaaactcc aataatcgga
    36361 aattactatc ttttcctcat tcctttttta aatcttcata cttcttctta tctttgtcct
    36421 aacctaataa cacaaatttt gaagaaaaac tcagtaaaac ctgattcttt ttttatcttc
    36481 acaacaacca actttttctt cagatccttt tactagaact tgtatacaaa aattgttgaa
    36541 tgctgatata atttgcggct acttgaaaaa acgatagatg taaaaagata aatagaatca
    36601 ttttttattc tatatcagtc tatgattcat tgcttatcaa tcacccaacc cattcgatgc
    36661 cgtttttttt agagaaattt aaaaaagacc gtacgaatct cgcattatcc gattactttt
    36721 ccagaaaaga tccccaattc tgtataaata accctatctt cttgttggtt tcattttctc
    36781 atataagaat tcagtcaatc tacccactat gaccattact acgtccaaca caactgacat
    36841 attaaccacg taagtattcc ctcacagatc tccgaatctc catggtatgg attcccatcc
    36901 tgacgctgca gaggacaaca tatttcatgt ggctctgtac ccttttgatt tggaaacact
    36961 acaaatcaat actcatctat aacttatcaa gcgaactttc gctaggcttc tagtgattac
    37021 taaattgttg gataacatac atagcccatt ttatctagct gttattcata acagttagtt
    37081 cttgtattac ttcttccgct tgtgttgcca ttgagcttgt caaaaagtta cttagaaaaa
    37141 agctgccagt aaagcaactt tcaacaaacc ctttttgttt cttatcatat caagaaacct
    37201 ccttttataa attttttcta taataaaaaa cgtcgataac ttaataagta tcgacgtaaa
    37261 ctatttttat atcccgtaaa tcaaaaattc taaagaaaga tttcctatat aagcatattg
    37321 gataattcga ttagtattgg gtatatctct accattctca tacctatcta tggaccattt
    37381 tgaaattgaa ggagaaaaaa gctttccaaa ctcctccctt tctagataat aatctttcct
    37441 aattttcctc atccttaatc ccatactaaa agaatttatc tttttaaaat cgctaactct
    37501 ttcatctttt ggaagtatat tcttataatc ttctccgtac ataaggtaag gaacagtgac
    37561 cttaccaaga taggcaatat tcattagttt ttcaatatct ggaatattaa tacctttttc
    37621 ccatctactt ataacatttt tgcctgtata gatggtaaag tttttaccaa atttttcaag
    37681 cgtctcttcc cggttctttc ggacttctcg tattcttttt cccataacaa ttttatcgtc
    37741 ttttgataaa ctaaatatct cactatcacg attaatctcc ttcaaaattt ctttttcatt
    37801 acttaataga aaaatatcga tttttttttg catttctttt gacggatttc tctttctatt
    37861 taacacataa ctgagtaata cttcactaat tcctatatta tcagctaact ctttgttaag
    37921 taacttattc atatctctgt atgcaattag tttaccgatg agaatctcaa tcttatcttc
    37981 ctcatctttt attagagttt ctatattcag aatcttgctt agagtattat ctacgtcttt
    38041 agggattttt cttgacgctt tttcatattt aactaaagta ctttgtgaaa tacctgctaa
    38101 atcagctaac tttttttgag atagtttttt ctcttttctt agagatttaa gaacccttcc
    38161 acttgaatta tcaaccatga aaaaagcacc tcctatgaga tgctttcatc atatactgac
    38221 agctaaaaat cttcaagaaa aatattccaa ttttgttaat tttagtatcg ccgtgataac
    38281 caaatgacag tttaaaatct tttatacaga taatcctcag tgttgcatct actcaaaaag
    38341 gtttatttaa taggtttttc gattgattat aagttatttc acactatctt gctcatccat
    38401 tttcagaacg gccatgaagg cttcttgcgg aacttcgacg gagccgattt gtttcatccg
    38461 cttcttccct tctttttgtt tctctaagag tttacgttta cgagaaacat ctccaccata
    38521 acatttagcc aagacgtttt tacgcaaggc tttgatgtct gaacgggcaa cgattttttg
    38581 tccaatagct gcttggattg gcacctcaaa ttgttggcgt gggattagtt ttttcagttt
    38641 ttcaacgatt gcttttccac gttcataggc aaagtctcgg tgaacgataa agcttaatgc
    38701 atcgactttt tctccattca gcaagatgtc catcttcact aacttgcttt tttgataacc
    38761 agacatttca taatccaatg aggcatagcc tttcgtgctt gatttcaatt tgtcgaaaaa
    38821 gtcaaagaca atttcagaaa gcggaatatt atagacgaca tttactcggt aatcatctaa
    38881 ataatccatc gtaatgaatt ctccccgttt tcgttgagaa agttccatta ctgcaccgac
    38941 aaaatcattt gggaccatga tctgtgcttt gacgaatggt tcttctacat cctgaatcgt
    39001 tacaggttca ggaaaatctg ccgggttatc tactgtagca gtcgtaccat ccgttttatt
    39061 aacatggtaa ataacggatg gtgcagtcgt gatcaattct agattgaatt cccgttccaa
    39121 gcgttcttgg acgacatcca tatgaagaag tccgaggaat ccacagcgga acccaaaacc
    39181 taatgcttga gaggtttctg gttcgaattg taaagcagca tcattcaatt gcagtttttc
    39241 taaagcttcc cgcaaatcgt tgtaacgaga agtatcaatt ggataaagac cacaataaac
    39301 catcggattc atttttcggt aacctggtaa agcttcagcg gcaggattgt ctgctaacgt
    39361 tactgtatcc cctactcgtg tatcttggac agttttgata cttgctgtga tataaccgac
    39421 atcaccgacc atcaaaaagt ctctagcaac tgcttttggc gagaagaccc cgacctctgt
    39481 aacatcaaat gttttgccat tactcatcag tttgattttg tctccgggtt tgaccatacc
    39541 gtccgtgatc cggacattta gaacaacacc gcggtaacta tcataaatag agtcaaagat
    39601 caaagctttt agcggtgcat ctaagtctcc acttggtgca ggaacatatt ccacgatctg
    39661 ctcgagaata tcttcaatcc caatccctga tttggcactg gctaatacag cttcactagc
    39721 atcgattccg attacgtctt cgatttcttg acggacacgt tcgggatctg ctgcgggtag
    39781 gtcgatttta ttgatgactg gcaggatctc caaatcattg tctaacgcta aataaacatt
    39841 ggccaatgtt tgtgcttcga ttccttgtgc tgcatcgacc actaaaacgg caccctcaca
    39901 ggcagcaagg cttcgagaga cttcataggt gaagtcgacg tgccctgggg tgtcaatcaa
    39961 gtgaaagata tatgtttctc catttttcgc tgtgtaattg agttctacag catttaattt
    40021 aatcgtgatt ccacgttcac gttccaaatc catcgagtct aacagctgat cttgcatttc
    40081 gcgagacgtc acagtgtgcg tcatttctaa aatacgatcg gccaatgtag atttcccatg
    40141 gtcgatatga gcgatgatcg aaaaattacg aatctgctct tggcgttctt tcattttctt
    40201 tatatccata gcgtctcctc ttttcatata atcagcactt tccattatac caacgaaaag
    40261 cagtaattta aagagttttt aattagtcag ctatagaagt tttttttgta gaattttgct
    40321 atactgttat caagctaata aatggagatg aaaaaatgga tcaaaaagat tttagagaaa
    40381 atcttgaatt aaaaccggtc actcaagaac atatcgatca attcaatgaa ttgctttcct
    40441 atgtgttcca agtgactgaa gcagacatcg aggaaagcgg gtttgagaat aaacgagctt
    40501 ttatccgttc caaaaaaccg attttggaag tatcgaaagt gttcggatgg ttccacgaaa
    40561 ataaactgat ctctcaaatc gctatctatc cttgtgaagt caatattcac ggtgcacgtt
    40621 ataaaatggg tggtgtgact ggagttggaa cttatccaga atatgctaac catggattga
    40681 tgcaagatct aattatcgtt gcattggata atatgcgcaa gaataagcaa tggatctctt
    40741 atttgtatcc ttacagtatc ccctactatc ggcgtaaagg atgggaaatc atgtctgata
    40801 aactttcctt taaaatcaga gatacacagc tacctaaaca aatcccagta agtggtatgg
    40861 ttgagcgatt accagttgat catccagacg tattttccgt ttatgacaaa tttgcccaac
    40921 aaaaccatgg tgctttattc cgcagtgatt ttcattggga agagtattgg cgtttcgaaa
    40981 atgaagatga gcgcacagcc gctgtctact acgatagcaa ccatgaacca agaggtgttc
    41041 tgttctactg ggtagctgaa gaaatatttc acgtaaaaga aatgttctat cttgatcaag
    41101 aagctcgaaa tggcttatgg aactttattt ccgcacattt ttccatgatc tactgggtac
    41161 atggagatat ttataaaaat gagcctttag catttttgat tgaagatagt cagatcaaag
    41221 agcagatcga accatatttt atggctcgga tcgttgatgt aaaagaattc cttcaacggt
    41281 ttccgtttgt tggaacagct gatgcttttc atttcatcat tgaagatcct gtcgctcctt
    41341 ggaataatgg tgtcttcgct ttaacatggg atgaacaggg acaagtccgt gtcttaaatg
    41401 aacctatagg aaaatccgtt cgactggata tccaaacatt gacttgcttg atgatgaatt
    41461 atcgccgtgc ttcttatttg gcaagaatcg aacggttaga aacagatgaa gaaacactga
    41521 agtcactaga gcgtatcatt ccaaacatgg aagcttactt tagcgattat ttttgataaa
    41581 aaggatgatg tttagatgaa aatttatttt gccgcaccaa tgtttgctaa aagtgatttg
    41641 ttatataacc aacagttagt taaagaaatc agagagctct cttctgagct ttcgatctat
    41701 cttcctcaag aaaatgaagc aatcaatgac aaaactgctt atgcagacag tcgaatgatt
    41761 gctcttgctg acacagaaaa agtgttggct agtgatttaa tgattgcttt actagatggg
    41821 ctgacgatcg atgccggggt agcatcggaa atcggtgtgg cttatgcgaa aggcatccca
    41881 gtgatcggct tatatactga tactcgacag caaggcggaa cccacccgaa aaaaatcgct
    41941 gcactacaag aaacagctga aaatcaattt cactacctga acttatatac aataggtttg
    42001 atcaaattga acggaaaagt ggtttcttca gaaattgagt tgctttcaga agtaaaaaga
    42061 tttttagatg gagggacttt cagtgattaa agaaatcaaa aaggtgcaaa tagctttact
    42121 tgcttttggt gtattcgttg tctgctataa tttttatgaa tttatcacac aaaaatattc
    42181 gacttctcaa ggaatcacat ttatcgtgga atctttatta gggattgcat tgattttcat
    42241 gccacaagtc attttgaaag ttttcaaact taaaatacca gcagcaatcg ttttgtttta
    42301 ctggtttttc ttgttcatct ctgtcttttt aggcacaggg atgcatttga tcagtatcat
    42361 ctctttttgg gacaaaatct tacatgcggt gagtccaatg gtcctgacag ctcttggcta
    42421 tggattgatt ggttacttga tgaaagatgc tgagatatca aaaaccagtc cttggttgtt
    42481 cctcttattc ggctttgcgt ttgctggact ttgcggcgtg ttttgggagt tctgggaatt
    42541 cttatgcgac cagttcttag gcatgaatct tcaacgtttt gctgcttctg atggcacact
    42601 gtttgtcgga cgagcagcat tgatggatac gatgggcgac ttgctgacaa atacgatagg
    42661 tgctgcctta atgggattgt ttgcatggtc tcaaagtaaa aaagatgagc gttactttga
    42721 aagttataaa ttagaaaaag tgaaaaatac ctaataaaag aggttgtgac agaagcgggc
    42781 agcttcaaga aatttcggct tatttttcga agaaactact tctggaacac tgtttatttg
    42841 aaaaaaggag gtctgacact agttgtgtca gacccctttt ttcttttata gtctcaatgg
    42901 aagattagac gatttcttct agctcttctg ttttgaaatg aagttgtcca tcaagtaaag
    42961 taatcgttac ttttgattta ggcataacat gtcctgcaac gatttcttta gctagtggtg
    43021 tttctacttc cttagtgata aatcgtttca atggtcttgc accatatgct ggttcataag
    43081 cattttcagc aatccacgtt ttggcttcat cactgattgt cagcaggatt tcttgatgtt
    43141 ccagacgttg tgccaattgc gccaccattt tgtcaacgat tcctttcacg ttgtctaggc
    43201 tcaatggtgt aaataaaatc gtgtcgtcga ttcggttcaa gaattctggt ttgaagtttc
    43261 cacgtaatag cgtgttgact tgttcagcca ctgcttctgg aatcgttcca tcagcagtca
    43321 cgccttccag cagtaactgt gaaccgatat tgctggtcat gatcagaacc gtatttttga
    43381 aatctactac tcgccctttt gaatcagtca agcgtccgtc atctaggact tgtaacaaga
    43441 tattgaagac atcgggatgc gctttttcga tttcatccaa caagacgatt gtataaggat
    43501 ttcgtctgac agcttccgtt aattgaccac cttcttcgta acctacatag cctggaggag
    43561 ctccgactag acgagacaca gcatgttttt ccatatattc actcatatcg atccgtacca
    43621 tatgatcttc tgagtcaaac aagttctcag ctaacgcttt cgctaattcg gtttttccga
    43681 caccagttgg tcctaggaaa aggaaagaac ctagaggacg atttgggtct tgcaatccag
    43741 cccgtgaacg aattaccgcg tcactgaccg catcaactgc ttcatcttga ccaatcactc
    43801 gtttatgaag tgtttcattc aatttaatta gtttttctcg ttcaccttct acaagtttcg
    43861 ttacggggat acccgttaat ctgccaacga cttgagcaat ctcattttcg gtaacggact
    43921 cttgaaccat cttaatctcg ctgtcttttg ctttagcttc taattccttc aattcttttt
    43981 ctaattgagg tatcgtacca tgacgcaata cagcagcgcg ttctaaatca tagttatttt
    44041 ctgcatcttc taattcgtgt tttgctttat cgatttcagc acgttttgct gaaacagaat
    44101 tgacttcttc tttttctgtt tcccactgca tcttcatcgc atttgcttct tcacgaagtt
    44161 cagctaattc ttcttgcaaa tttttcaagc gttttttact tgcatcatct gattcttttt
    44221 tcagtgcagc ttcttcgatt tccaactgca tcaaacgtct agtaacttga tctaattccg
    44281 ttggcataga gttcatttct acccgaatcg ttgcactggc ttcatcgatc aaatcgattg
    44341 ctttgtccgg taagaaccga tcagtaatat aacggtcaga taaagtagct gctgctacta
    44401 atgcgttgtc atggatgtta acaccatggt ggatctcaaa acgttctttt aatccacgca
    44461 gaatactgat cgtatcttcc actgttggtt ctttgactaa taccttttga aagcgacgtt
    44521 ccaacgcttt gtctttttcc atatattgac ggtactcatc taaagttgtg gcaccgatca
    44581 aatgtaattc accgcgagca agcattggtt tcaacagatt tccggcatcc atacttcctt
    44641 ctgtttttcc tgcccctacg atattgtgga tttcatcgat gaacaagagg atacgtccat
    44701 cacttttttt gacttctttc aagactgctt ttagtctttc ttcaaattca ccacggaatt
    44761 ttgctccagc gatcaatgca cccatatcta atgaaaaaat cgttttatct tttaaatttt
    44821 caggcacatc tttgcgtacg atacgttgag caagcccttc aacgattgcc gttttcccta
    44881 caccgggttc tcctatcaga acaggattat tttttgtttt tcgtgaaaga atacggatga
    44941 catcacggat ttcttcatca cgaccgatga ttggatccat ttttccgctt ttgacttgct
    45001 gtactaaatc aacgccgtat ttttctaatg ctttgtattg ttcttcttgg ttttgtgagg
    45061 tcactctttc tcctcctctc atctcttcga tgtttttacg caattctttt tcagataatc
    45121 cgtttttatt caaataaaca gttaacggat aattttttaa tttcatcaac gctaaaataa
    45181 cgacttccgt tgaaagaaac tcgtcgccga agctttcacg tatttggtct gcttcattca
    45241 ataatcgaaa gaaattctgg ctgaggtttt gtccatattg gacgtttcca ccagaaacaa
    45301 ctggatactc atccagtaga cgatcaattt catgttcaaa agattcgaca tccaatccgg
    45361 catccgtata gaaatttcga ccaaaatgat tagattgtaa aaagattttc catacatggg
    45421 caatgtcgat atcttgatgt tttcgagtga ccgcaatctg ttgagcttca gcaatcgctt
    45481 cttgaagcgt cgtagtcatt ttctcaatat tcatacaggt ataccctcct aattaaaggt
    45541 aagatcactt atttctttga tctttacaaa aactattata tcgctttggt caattttggt
    45601 caaaatatac acatagtttt ttgacttttt ttctcgactt atataaatct taaaaaaaca
    45661 aaattgattg tatgtatata aaaaaagagc caaacaaacg taaaattatc ttatccataa
    45721 cgtttttctt tgatttgata aaaagggtag atcttataaa a
//
LOCUS       DACJGB010000016        40814 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.016, whole genome shotgun sequence.
ACCESSION   DACJGB010000016 DACJGB010000000
VERSION     DACJGB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.016"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            23..646
                     /locus_tag="IYI34_000952"
     CDS             23..646
                     /locus_tag="IYI34_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558837.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IYI34_000953"
     CDS             1096..2145
                     /locus_tag="IYI34_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0558838.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IYI34_000954"
     CDS             2142..3023
                     /locus_tag="IYI34_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558839.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IYI34_000955"
     CDS             3026..3796
                     /locus_tag="IYI34_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0558840.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IYI34_000956"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IYI34_000956"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAR0558841.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IYI34_000957"
     CDS             5107..6270
                     /locus_tag="IYI34_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAR0558842.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IYI34_000958"
     CDS             6387..6797
                     /locus_tag="IYI34_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAR0558843.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9198
                     /gene="topB"
                     /locus_tag="IYI34_000959"
                     /pseudo
     CDS             6971..9198
                     /gene="topB"
                     /locus_tag="IYI34_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            9336..9653
                     /locus_tag="IYI34_000960"
     CDS             9336..9653
                     /locus_tag="IYI34_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558844.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9813..10049
                     /locus_tag="IYI34_000961"
     CDS             9813..10049
                     /locus_tag="IYI34_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR0558845.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10046..10444
                     /locus_tag="IYI34_000962"
     CDS             10046..10444
                     /locus_tag="IYI34_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAR0558846.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11111..12055)
                     /locus_tag="IYI34_000963"
     CDS             complement(11111..12055)
                     /locus_tag="IYI34_000963"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAR0558847.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12351..13295)
                     /locus_tag="IYI34_000964"
     CDS             complement(12351..13295)
                     /locus_tag="IYI34_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558848.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13516..14730
                     /locus_tag="IYI34_000965"
     CDS             13516..14730
                     /locus_tag="IYI34_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0558849.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14752..16767
                     /locus_tag="IYI34_000966"
     CDS             14752..16767
                     /locus_tag="IYI34_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAR0558850.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16850..18223)
                     /locus_tag="IYI34_000967"
     CDS             complement(16850..18223)
                     /locus_tag="IYI34_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAR0558851.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18249..18533)
                     /locus_tag="IYI34_000968"
     CDS             complement(18249..18533)
                     /locus_tag="IYI34_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0558852.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18535..20550)
                     /locus_tag="IYI34_000969"
     CDS             complement(18535..20550)
                     /locus_tag="IYI34_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR0558853.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20729..21034)
                     /locus_tag="IYI34_000970"
     CDS             complement(20729..21034)
                     /locus_tag="IYI34_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAR0558854.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21123..22154)
                     /locus_tag="IYI34_000971"
     CDS             complement(21123..22154)
                     /locus_tag="IYI34_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAR0558855.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22361..23236
                     /locus_tag="IYI34_000972"
     CDS             22361..23236
                     /locus_tag="IYI34_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR0558856.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23388..24230)
                     /locus_tag="IYI34_000973"
     CDS             complement(23388..24230)
                     /locus_tag="IYI34_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR0558857.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24322..26199)
                     /locus_tag="IYI34_000974"
     CDS             complement(24322..26199)
                     /locus_tag="IYI34_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAR0558858.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26196..27617)
                     /locus_tag="IYI34_000975"
     CDS             complement(26196..27617)
                     /locus_tag="IYI34_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAR0558859.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27620..31009)
                     /locus_tag="IYI34_000976"
     CDS             complement(27620..31009)
                     /locus_tag="IYI34_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAR0558860.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31266..32657
                     /locus_tag="IYI34_000977"
     CDS             31266..32657
                     /locus_tag="IYI34_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0558861.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32730..33377)
                     /locus_tag="IYI34_000978"
     CDS             complement(32730..33377)
                     /locus_tag="IYI34_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0558862.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33385..34071)
                     /locus_tag="IYI34_000979"
     CDS             complement(33385..34071)
                     /locus_tag="IYI34_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558863.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34077..35150)
                     /locus_tag="IYI34_000980"
     CDS             complement(34077..35150)
                     /locus_tag="IYI34_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAR0558864.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35372..36046
                     /locus_tag="IYI34_000981"
     CDS             35372..36046
                     /locus_tag="IYI34_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0558865.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36051..37391
                     /locus_tag="IYI34_000982"
     CDS             36051..37391
                     /locus_tag="IYI34_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0558866.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37540..38526)
                     /locus_tag="IYI34_000983"
     CDS             complement(37540..38526)
                     /locus_tag="IYI34_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAR0558867.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39250..40281
                     /locus_tag="IYI34_000984"
     CDS             39250..40281
                     /locus_tag="IYI34_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR0558868.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40390..>40814
                     /locus_tag="IYI34_000985"
     CDS             40390..>40814
                     /locus_tag="IYI34_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAR0558869.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
BASE COUNT        12669 a         7414 c         7663 g        13068 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct cacaagcaaa agcctatgcc
     7021 gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt
     7081 gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga
     7141 gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc
     7201 ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg
     7261 cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg
     7321 tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg
     7381 atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag
     7441 aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga
     7501 atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt
     7561 tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc
     7621 aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg
     7681 cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag
     7741 gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg
     7801 aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag
     7861 atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac
     7921 gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat
     7981 cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag
     8041 acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat
     8101 gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta
     8161 cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat
     8221 atctacgaag agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga
     8281 aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa
     8341 gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg
     8401 gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa
     8461 actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga
     8521 atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc
     8581 gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta
     8641 gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga
     8701 aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc
     8761 ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt
     8821 agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca
     8881 aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa
     8941 tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga
     9001 gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg
     9061 aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg
     9121 atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat
     9181 gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga
     9241 gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag
     9301 gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa
     9361 attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg
     9421 attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga
     9481 ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa
     9541 gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca
     9601 ttatttattt tctaccagtt attacacaag tatttcatca atattttggt taagaatacg
     9661 agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa
     9721 agcaaaataa aagtatatac attgattaaa agtatatact tggatataat gtacttacta
     9781 tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta
     9841 gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg
     9901 tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca
     9961 aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag
    10021 acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat
    10081 caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa
    10141 tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc
    10201 tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca
    10261 taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg
    10321 tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc
    10381 attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa
    10441 atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg
    10501 atttttttat gtttaatcat acaaaatagt tttttcataa aaatatggaa aatatggatt
    10561 aattttgaaa taattgattt aaaaggaata caaaagagtg aatagtagga aatagtggtt
    10621 tagaactagt gggctgattt tttatatatt ttagtttaca taatatatat tatacaaagt
    10681 agatttaaaa aatgataaac ctctctcgaa cggtttttta tcaaaaatcg tcatttttcc
    10741 atcaaaatct gcttatgcaa aattataaaa aaattttgca gtttttctta ctttttttgt
    10801 cgtttaattg aatgataaaa accagtttct ttattcagtt aatcaaatat gaacgaatta
    10861 gatatgaatc attgtatttg ccgttgcgga aggatttttt aattaaccac agataaacat
    10921 tgataacgat tcgcttattg taatcaacag attaaatttc atatgttcat atgtacggat
    10981 acattttcgg accgattaat tttaaccatt ctgtttattt gcttacctat tagatatcaa
    11041 taaaaaactg cttaacagct atttcagctg ttaagcagtt ttaggatttt tagttcatat
    11101 tcattgtttc ttataataca gtgttcaata cttctcttaa agcacttgtt gatttgctga
    11161 acattgcttt ttcttcttcg ttgattgata attcaacaac ttcacgaaca ccattttcgt
    11221 caacgattga tggcacacct gtaaagatat ctttttcgtc gtattcacca gttaaataag
    11281 cagatacagg caatacagct tgttcgttat tcaagatagc ttttacgata cgtgcagtac
    11341 tcataccaat accatagtaa gttgcttttt tacgatcaat gatctcataa gcagcattac
    11401 ggactttatc agcaatcaca tctaattcat cttgggcaat acgatggtct ttttctacaa
    11461 tttcaaacac tggtttacca ccgacagttg tatgtgacca tgcagcaact tctgaatcac
    11521 catgttcacc taagatatag ccgtggacgc tgcgtgggtc aacttttaat ttcaatgcga
    11581 tttctttacg gaaacgagtt gtatccaatg ttgttccagt accgataaca cgtgatgttg
    11641 gcagaccaga ttcgttccaa gcgatatatg tcaaaatatc tactgggttt gaagcaacaa
    11701 cgataatacc gtcgaatcct gatcccatga tttcttttac gatttggcgc atgattgacg
    11761 catttgtttt aactaaatct aaacgagatt gaccaggttt ttggttgata ccagctgtga
    11821 taacaacgat atcagcatct ttacattctt catagccacc tgaccataca gccacatttt
    11881 catcgcccca agccatacca tctaaaaggt ccaatgcttc cccttctgct ttttcttgat
    11941 tcacatcgat caatactaat tcattagaga ttccttggtt gatcattgca taagcgatac
    12001 ttgttccaac aaaacctgta ccgacgatta ctactttacg acttgttttt ttcatgaata
    12061 aacagctcct tttcataact atttgttact ttcatgaaaa gtatagtctt gtttattcat
    12121 tttatcaaca tgttaattat aatagttgtt attataattt ttgatggaaa cgaattcctg
    12181 aatacgcata acagataaat gacaaatgtt tacacctata tcccctgata atacaaccaa
    12241 aagtttagaa ctgaaaataa atccaaaaaa attcgctgca acgatataat aagacagcaa
    12301 tcgcacctaa aacaattttc cttcatatgc tttagtgaaa gattttcttc ttaatgttta
    12361 tttctccatt cattgggagt acatgttgaa tacttcttga ataatttgaa taaagaagtc
    12421 gtttgtttat atccgatacg attagcgatt tcttccaacg aaaaactcgt ttcttgaatc
    12481 agttccaacg caaagtcgaa ttttttcttt agaaccagtt cttgaaagga agcatcaaaa
    12541 tacattttca atttattact aagatagacc ggattgtagc caaaatgagc tgctgtttct
    12601 tttaacgtac aagaagtaaa atgcttatcg atataatcta atactctaag catctcattt
    12661 tctgcgcatc catctgctga tgaaaagctt tgttgttcta ataaatgagt caattcgatc
    12721 agtaatagtg atgtatagag atttaacgaa cggatcttat gactgtatgt atcaaaataa
    12781 atcaacagca tattttcaat caaatgcgtc aaacgacttg tttttccgcc gcgaaaatga
    12841 atgaattgtg tatgttcccc tttttctttt gatgcattta tcaaaaaaga agaaagtaga
    12901 ttatttgtgc ttgccaaatg attcaatatc tctgtagata aggtttcttc ttttaataag
    12961 atgttgatta aaatatcctc ttgccctgtg tattcgattc tttgagtagc ttctttatca
    13021 atgatcacca cgtcactttc ttccatctct atcctttctc cattaataat ttgtaaacat
    13081 gtaccgctat acatataatt gatttcaaca aaatcatgtg tatgctcggg aacttccgca
    13141 tatcgacggt gtttactgat atgaatattt ccctgttgaa atagatcccc tcggtggatt
    13201 tttggcggtg tctgaagatg gtaagctaag tttaaatcat tgatattcat tccttgttca
    13261 cgctgcagag tttcgattga ttcttcgtta cgcaaaaaaa atgtctaatt cagctagttc
    13321 caattgactc cctccttttt gtaagcggtt tctttcgctt attagttcac gctgtactgc
    13381 tattatatgc aaagctattt ttggtttcat aaagactttc tatggttggg acatgaactt
    13441 cctgtgattc cgctattgag agaataacca cttcatacta tatttttgat aagcgcttac
    13501 aaaaatgaaa ggaacatgct tatgaataaa aagatgatga aattttctct gctgtcagtc
    13561 tctcttattt tgacttctgc tggtgcagtc acgtctgcaa ttcccagtat gtcagctact
    13621 tatgccacgc gttctttatc tgctgttgaa ttattaacga ctgctccctc ttttatggtc
    13681 actatttttg ttttgattag tagttttatt gctaggaaaa tcggacaaaa acaaactgtg
    13741 atcatcggat tagtgattgc atcgatttgt ggtatcctcc ccctcttctc acaaagttac
    13801 agtattgtct tagcttcaag agctggacta ggtattggct ttggattgat caattctttg
    13861 gcagtcagca tgattgctgc cttttatcac ggagatgaac gagcctctat gattggtttt
    13921 caaagtgcat ttcaaggatt tggtgcagct ttaatgacat ttgttgcagg acagctagta
    13981 cagttcggtt ggcaatatac ctttttagtc tacgccatca gcttacctat tcttgtcttg
    14041 tttgttttgt ttgttcctac acctgaaaca actgaacttc aatcgcatgg cacagcccca
    14101 acaagacaag taatcaaaac gaatcctaaa atgattagtt atggtatttt tctcttgctg
    14161 gtcatcgtgg tatataatgc tgtatcgatt aaattagcca cagtcctgac gacttattct
    14221 ctaggaaacg aaactaatgc agcgaatatc ttaacaatca tgcaattggc aagtatggct
    14281 gccggagttt gttttggctg gcttcagaca aaagcacatc gctatatgct tacactttct
    14341 ttactactga tgggaatcgg ctttgtattg attgccactg tgataaacct gtatgtgatt
    14401 gggtttggtg cgatcctaac aggaatttct ttttcgttat tcattcctta cttattcaac
    14461 caagtaagta tccaagcgcc caagtacgcg gagagtttca atacttcaat cttattggtt
    14521 ggcgctaatt taggtacttt cattgcacca tatggtttgc tctttctctc ttttgcttct
    14581 cttgggagta aaacacttgc tgttttcgtc aatggcagta tcttgctgtt gattagcgcg
    14641 cttattagcc ttctagtagt acacaggacg aatagaaaga cagtgataag agtagatgaa
    14701 gaaataaaag aacaattgac aacaaaataa atttgatgga ggtcgtgtaa aatgacaaat
    14761 gaaaaacata aagatagatg gctttggtat cctggagact ttgagatccg ccatggactg
    14821 ctacaaaatt tccaacggga agaacgaggt tttgactggc cagcgtactg gtacatggat
    14881 gattgccatc ggaacgtcaa gtttaagaga tattactttc ttgatcaacc ttccatgttt
    14941 aaagtgacga tccaaggagt tgggtatgta gaaattaatg gacaaaaaca tccatgtgga
    15001 aaatggctga cttgtccagc tggtaaagca aaaattcgta tctttgtagg acatacatca
    15061 gggctaccag cgatgtttat cgagggagac gaagtaaaat cagatattgg ctggacagca
    15121 agtaatttta ttgaagaata tccagctggc tggagtccgc tttatgtcga tatcgcaaaa
    15181 gatccaaatc aaatctatta tcaaaaagaa tgcgtccatc cagtcactga aacggagatt
    15241 ggcggaggaa tcttattcga ttttgggcga gcagtgaatg gctgtgtaga actaatatct
    15301 ctggatgacc atatcgaatc tatcactcta tgttatggag agtccgatgt agaagctatg
    15361 gatatcgatt attgctacta taagcaaata ggaatgacaa gaaaaatgaa agctcgtaaa
    15421 cgcgcttttc gctatttgtt tataccagag gtttctactg gaatggttca acttcaagtt
    15481 tttcatgaat ttttatctct ttcgaaacaa gctgcttttt caagcgattc ttctttactg
    15541 aatcagatct ggtcagtatc agaagaagcc ttcaagttat gtagtgattt gttttttatc
    15601 gacggtatca aacgagatcg ctggatttgg tctggagatg cgtatcaatc attcatgatc
    15661 aatcaatatc ttttttttga cgaggaaatc aataaacgaa cgatattagc attaagagga
    15721 caaaatgaaa tcaagcagca tatgaatact atcgttgact attcgatctt gtggttgatt
    15781 ggtatcgaaa accactatat gatgacgaaa gactatgaat ttttacgcat cgtctaccct
    15841 aagatgcaat cattaatgag atacttgatg gagcaaacga atgaattagg gtttatttat
    15901 gggagagcaa aagattggat atttattgat tggtctgaaa tggataaaga aggaacgctt
    15961 gctgcagaac aagtcttatt actgaaagcc tatcattcca tgatcacttg cggagatatc
    16021 ttagctgaaa catatggaga gaacgaaaat tatcatactc atctctatca agaaaaatat
    16081 caactattga aacaaaacct atttgactac ttttgggatg aagaacttgg cgcttttatc
    16141 gattgctatg aatctgggag aagaaatgtg acgcgtcatg cgaatatttt cgctgtttta
    16201 ttcgattttg ctaacaaaga ccaacagcaa tcgatcttaa agaatgtctt attgaatgac
    16261 actatcacac agattactac tccatatttc aaattctatg aacaagatgc tttatgcaaa
    16321 ctcggacaga cgaatatcgt ctaccaaacg attcttgatt attggggagg gatgttatct
    16381 catgatgcgg taactttctg ggaagaatac gaccctactc aaacaggaaa tgaaaagtac
    16441 agtatgtatg gagatcctta tggaaaaagc ttatgccatg catggggagc aagcccaatc
    16501 tatcttttag gacgctattt tatcggtttg tatccaacaa agccaggtta tgaagaattt
    16561 atgatacaac ctaatctaga ttcatttgaa acactgaatt gtacgctacc tatcaaagat
    16621 ggaaagattt cgcttgaaaa aacaaaagat aagatcgtga ttacttctga tagaacaggc
    16681 ggaacagtga aaatgaatgg taaaagttat cctcttctag caaaacagcc acttgtctta
    16741 acaagctatt caaaaataaa tgcataaaac aaaaaactgc ttgcgacata tcagaagact
    16801 ttaaatcttc ttctgatatt gtccaagcaa tttttatttt gaagtttgct taattctgtt
    16861 ctttagaaac aatgtttgtt ctttttccgg caaaaagaaa gctagataat acgacaagta
    16921 aaaagaaagc gatgattcca actgtcaagg ctatttgacc gtattctcct agtttcccaa
    16981 gtacaagtcc tggtactaga taatcagtat cggcaaatgt agaagaagcg attttaagat
    17041 ctcctaatac tggaaggata aataatggca gccaactaat caataagcta ttgaaagcag
    17101 aacctaatac cgcccctctt ctaccgccag tcgagttgcc gaagacacca gaagcagctc
    17161 cgcagaagaa atgtcctact accccaggaa tgataactgt agtttgcaag cctatcatag
    17221 caaacatgga tagtgttcct acaaagaagc taacaaaaaa gccaattaat acagcattag
    17281 gtgcatatgg aaaaacgata ggaacatcta atgctggttt agaatcgggt acgagttttt
    17341 tagcaatccc ttggaatgct ggaacgattt ctgccaacac cattcgaacc ccttgaagga
    17401 cgataacaaa cccagctgtg aatgttccag cttggatcag tgcaaagatt acagcgttgt
    17461 ctccattgct tagttctctt tcaacaaatc caggaccagc taacaaagca agtgcgacat
    17521 atacgatcga catcaagagc atgatcgata cagtagaatc tcgcaaaaag ctgaaacttt
    17581 taggaatctt tatgtcctct gtagaacggt ctttgtttcc aaaaaacttt ccgatccatc
    17641 cactagaagc ataaccgata tttccggtat gccctaacgc aacttgattg tttccagtga
    17701 tgttcttcat aaacggttga caaagagcag gcgtaatagt taatagtgtt ccttcgaaga
    17761 ccccgcctaa taagatcatc cacaaatttg cagtaaaccc cgcgcttact aaaatgactg
    17821 ctgttagaca agaaacataa agcattgctt gacctgtaag aaaaatgaat ttatatcttg
    17881 tgaatctagc aagtaaaata ttcacgatca tccctactag catgatcaaa gcagtaggtg
    17941 taccgtattg ttgcaaagca atcgccacta ctgcttcatt gctaggaact actccttgag
    18001 tatgcagagc tgtttggaac atcgaagcaa aaggggttag tgaattactg attaaaccag
    18061 caccgccgat cagtactagg aatccgacaa atgttttgat agttccttgg atcacttctg
    18121 aaaatttctt cttctgaaga acgagaccaa gcagtgcaat caaagaaacg aggatggagg
    18181 gctgactgac gattgacaca aacaaattca acaatttatc cattgatagt tcctcccttg
    18241 aatcattttt attcttttga caagatcttc gttacttttt cttttacttc ttcgctgtca
    18301 atgatcgatt gcaaaggtac gagcttttca tcaggtatag aagttagcgt atctttcaaa
    18361 gaatgctcaa tgaagaaata atcagccata tcgctagata cacttccaac atccgtatga
    18421 cttacttcaa actcgtccac atctgctcca agatctgcaa gtgcttgttt gatgttcatc
    18481 tctaccataa aacttgttcc taatccactt tgacatactg caaaaatttt catatcattc
    18541 gtctcctttt cttatgatcg ctaatatttc ttcttttgtt gatgcgtgta ataaatcttg
    18601 aagattttgt ttattagaaa gtagctttgt taaatttgcc aaagcccgaa gatgtgcctc
    18661 attatcgata gcagctaaac agatgaacaa actaatggga tgggctggat tatcaagcag
    18721 atccacaggt tgttgaagtt tcagcaaaga catccctact tcgtttacgc catcttctgg
    18781 acgagcatga ggtaatgcga tgtgatcgcc aatatggata aacgcaccat actgctccac
    18841 gcgctcgatc atagctttga tataactttc ttctatttta ttttctgata atagcggctg
    18901 tgcggcaaat gtgattgctt cttgccagtt ttttggttga tccgataatt ggatcatttc
    18961 ttctgttaat aattcagata gcataggacg ttgatcctcc ttcttttcta aaagtttgtt
    19021 cattttacga tttaatactt gatagagctt ttcttcgtct acgccttctt ttaattcgat
    19081 ataaggcagt agcgccgaca acaattcttg ggcagaaggc attgaaaatc ctggtatcag
    19141 ccaatctctt tgtacttgat tgatcagctg atttttttcc aagtcagtca tgattggatg
    19201 cagaacatag agagactttt tcttatcagt aagtgtgaga ggtacagtag aaaaaataat
    19261 atcgtaagaa gtttcgtcaa cctcgtgtaa gcggtcagct gaattagtct ctgtaaaaaa
    19321 gatcatcggg aataattgct gaagttcact ttttaagatc aatgaagagc taatgccact
    19381 tggacagacg atcacggctc ttatttttct atttctttct tctgcgtccg cttttctgat
    19441 ctctccgcca aatagtatag tgaaaaaacc aatctcttct tcagggatgc tcttccctat
    19501 tcgtctttct aacggaagta atgcttttct tgtcatttcg tagatggaag catattgatg
    19561 cttaatttga tcgatcatca cattaggtaa ataaaaagaa taggaaatac gaaaataggc
    19621 agggaccaag tgataaaaga cattcatcaa taattcacga aacgaagcaa attctacggc
    19681 catcaggaat tccatgcgat ggatgacttc tgaagaacat tccagcaaaa aatcaagtgc
    19741 aggctcctgg acatctcctt caacgatcga tgctaagcaa agagagaaaa atataatctc
    19801 cgaattttga tgttcggaat agccaaggcc agtcaatact tgtcgactaa tgtcagtgac
    19861 tgcaagagaa gccaacagtt cagcatcttt taccagttcc atggatgttt ttccaattcg
    19921 aatggttgtc aaactcaaat aataagctaa ttcatccatt cgatttggtg cgaatcgaag
    19981 atctgatagt gaaatcgttt tatctagcaa atctctaact ttggcgtaat aatcaatgga
    20041 aagggttgcg gttaaatacg tgagcccaaa tcttccagca agtgactgca tcaaacgagc
    20101 agtgcgattt tttgcttctc ttcttaatat tgcttctgac cctagcagcc taaaaccagt
    20161 tttcctagta tacgacagct gacaatcttc cacttctaat tcatttctta tctttttgat
    20221 atctgacagg actgtccctt tagaaacctg aagaaaatct tgaaaatgaa aaacagaaac
    20281 agacgttgca gtcgaataaa ccatcaaata aagaagatct cttcgctctt gttcggtata
    20341 cacagcttca aatcgagtaa tatccaaaaa tttttctgcc cagtcgattg ctcggaattt
    20401 ctttatacgg atacgggtat catccacata ttcggcaaaa ggagttaatt gtttgatcgc
    20461 ctcgcttgta tttacttcga tgatctctgc tgctttttct agactgattt ctcttggtgt
    20521 tttcaggaga taactaattt tgtagttcac agcgctgttc acctcctact tatattctag
    20581 tatggtagcg cttttaaatc gaggggtgtt tggtacaact tacagttgga ttttttttac
    20641 gactgtaccg aacgatttct atcgaatgga tacagaaaaa aagcaggtga ccaaatgtct
    20701 cctgctttta tgaattgatt tcgtccgatc aatgatgatc atgtttattt tttgaggtca
    20761 gttcttcaaa tttttctttg acatcttcaa ccgctttttc gatatctttt tcaaagtgat
    20821 tgggctcatt aggctttgtt tcctcttcta cagaatttga accagataaa gctgtttctt
    20881 tattttctgt ttcaccagag tttgtgccag ctaatgattc ttctttgtct tctgtttcac
    20941 cagaattagt tcctgctaaa gccacttctt cttctgtttt aggggccatt gaacctctta
    21001 atcgtttgtg tttttcaccg tcataatttg tcatgttggt tcctcctagt agttctatac
    21061 tttttcatcg tacaataaga cagtttatgc gtcaaaaaac aaacccgatt cttacaaata
    21121 tgtcatattt ttccttctga ttcttgaatg cttttgattc cttcttccaa aattttcatt
    21181 tgtaagattg tttcttcatc tttgatgact ttttctgcgc cattaactaa tgtctcgtaa
    21241 atcccttcgt agacacggct gtaatcacca acctcggaaa ttactttttc ttcatgataa
    21301 ctcccttgtt catctatata ggtcaataca ccataatgtt cgggcaaatc gataccaaag
    21361 tcttcatgat caggcatgta aaacattttc agatgttctt cttgtcgatc ttttgtttgt
    21421 ttcacgaaca tccctttttt tccgtaaacg acgaaacttg gacgttcttt gatgcggaaa
    21481 taactggatt ttatcgaaat tttcatattt ccatagaaaa aatccagatc gaagtaatca
    21541 ttcattcgtc ctttccctaa aagttgacgt acatcatagc tgatatcatc tggttcgccg
    21601 aaataagaaa gaacttgatc tactgtatga caagcatgtc catataaata gctgttcgca
    21661 gtagaaaatt catgcgtatt ttctggtact tccggtctga aataatcata atgcatttct
    21721 acttctaaaa tatcccctaa ttttccgctt tcgattactt tttggactgt caagaagtca
    21781 gaatcgaacc gtctgttttg gtaacattgg ataaatagac ctttttcttt tgctaatgca
    21841 aataactctt ctgcttcctt tgacgtttct gcaaaaggtt tctctactag tacattctta
    21901 ccatgttcga gtgccatttt tccatattca taatgaaatt gactaggtgt agtgatgaca
    21961 actaattgga tctcaggatc atcccaaaga tcagacagct gatcagtata ttcgatgtct
    22021 tcgaatgttt tccacactgg ttcagatggg gaaaaaattt ttttgatatt gattttgttc
    22081 gtaagttttc ttgaaaaagg caaatgatag cgatttgtac tctttccatt gccaagataa
    22141 gcgattgtaa gcatgagtgg tgcctccttt tattgatgtt ttcagtatat cctagaaaag
    22201 cgcagaaagt atttataata gctattctgg taaaaaagtt cagaaaaaca cttgttatca
    22261 ttcggtttgg tgaaattaat gttcaaagta aggataaaaa tcaaaaagat agtagcggaa
    22321 ccaagtgttt atcagtaaga tagaatttgg aggtcagatc atgcttattg aagaaaaact
    22381 aacaaaacaa gagttgttta ccacgacaga gaaacggatt gtcgattaca tccgtaggaa
    22441 tatcgaagct gcagtctaca tgacgattga agaattagca aaagctacgt atacttccca
    22501 ttcagctatc attcgtttat gcaaaaaaat gggattcagc ggatttaaag aattccgttt
    22561 cgagctgggg cgggaagttc atcaattgat tgctcagttc aatcaaacga ttgatcctaa
    22621 ttttcctttt tcaagtgaag atagcactca gcaaattgca aagaaaatgg cggatttatc
    22681 gatccagtca ataaaaaaag cacaattgca gattgaacat gaagatttag atcaaatcgg
    22741 taaagtattg aacaaggcac agcgaatctt tctttttgca aaaggtgatt ctcaaatcac
    22801 ggcaaggaaa ttccagaata aaatggtcaa gttgaataag tttttgatca tggctgaaga
    22861 atacagtgat tcttcttgga atgcagccaa tttgactagc gaggattgcg ccatttttat
    22921 tagctacagc ggtcgcatcc atcattatga acgaataatg acctatttga agcatgtaga
    22981 ggcccctacc ttgctgatta ccggaaatca gcattcagaa atggccaaac aggctgatat
    23041 gtgtcttgtt atatcgcagg aggaatatga ttttgctaaa gtagcgactt tctcctctca
    23101 aattgccttc gattacgtat taaatacgtt attctcagtg atatattctc aaaattttga
    23161 agagaatatc cgcaacttga aaaacaaaca agaattgatg caaaaaggcc ttcttaaaga
    23221 atatcggaaa acttaagaaa agatcctcat ttagtaataa gaaagatagt gtttgatagg
    23281 ctctctttga tatcatcata aaaacaggct gaagttttct ttttcaaacc tcagcctgtt
    23341 tgtttacgga acgattcgaa tagatatatt atagaaatga ggaatagcta acttctgttt
    23401 cttattttga acgattgacg aaaaagatat ataagaagga ggaaaatcag tatacatgca
    23461 gctaacaatc catacaggaa aaatcgtttg ttctcggata ctttctgtac ttctttttga
    23521 acttcttttg catgataagg aacccggtgc cctctgacaa gtaagcgatg actgttgacc
    23581 atataaggtg tacaagttag gagtgtcacg aaatcctgcc ctttttcgat tctcagatct
    23641 tttgtatcag tcggttctac cgtcttgatc tgatcgactt gataagcgag tgtccgtcca
    23701 ttgatttcga tatagaaaga atcacctaaa gtcagtttcg gtaagtctgt gaacaacttt
    23761 gcttgaggca gtcctcgatg gctggaaata acggcatgtg tattttctcc ccccgtagga
    23821 taagacgttc cttccaataa tgaggcacct ttttctagca gcacagtatt cgtttcatca
    23881 aaaattggca aatggacatt gatctcaggg atcgtcaaaa taccgatcgt atgtttctca
    23941 aaataggttc tatccttttt cgcaggttct tttttcttag taaagggatc tgcacctgga
    24001 tttccacctt cttttgctaa ttgctggttt ttctttgtca tgttttcttg gattttagcc
    24061 atgacttctt cattttcttt gaccgcttgt tgctgataat gagagatgat ctgctgatcc
    24121 agataattat tcagcgcgtc gctaacaaat ggataagcta gtatccctat tcctgagaga
    24181 agcaagaaaa gcatcagcag atctagaagt ttccttttct ttggtttcat ttgtgttctc
    24241 cttgtgatga ctgtttgata ataaaagaaa aaggagcatt tgcgtaatcc gctgctcctt
    24301 tttccaattc ttgcaaaaag actaaatctg actgtgcttg cgtctagcaa agtacagtcc
    24361 agcaatcagt agaaggacta ctcctgcacc gatatacaca tagattccct taccgcctgt
    24421 tgaaggaagt gtacctttgt gtttatttgg tattttttca ggagaaatca actgtcctgc
    24481 tgttacataa gattgttcat cgatagtaaa tgctacacgg tttgttaatg gaacatattt
    24541 ttctggcgct ttcgtttctt ccagatagta cgtgccatat tttagacctg tcacatcgat
    24601 taaaccgtta cttgtggttg taaaaaccgt tgctgattct ttcgccgata cccagctgac
    24661 ggcttttgtg gatgggtcga tcgataaata tttcgctgtg tcactatctt gatcacgaac
    24721 gacgaattct gctccagcaa gtgtttggtc tgatgtaacg tcaccatcta ctttaacaaa
    24781 tcgtttgccc ccagtaacga catcgactga cggtggtgtt tgatcatttg tatgcccgtt
    24841 atcgacattt gcttggttag aaaatccttt ggttggatct gctttttcgt tcaaatgcat
    24901 atagtaaacg aatttcaatg taccgccagg agttaatgaa ggaatataat tcggatcaac
    24961 tgaaaccgtg aatccgtctg tttgctcagt gacagaataa tttactgggt cgatttcttt
    25021 atttccatca tataaggcat aagcatacgt tcctgaagaa tcattatcaa atgttaaagc
    25081 agcgtcatga gtatcgataa gtttgaatgt tgtgtacttg ttttgcgttc cttctttatc
    25141 agcgatacct aatgggatat tgacagaaat ctcatattga attttttcac cgattgcgac
    25201 atcttttcca ttcaattgag gtgttgtttt atcaacttta gacgtatcat ttttgatggt
    25261 cttttctact ggtgtttggc tatttgccag gatctcaaaa ggcgtgattg tttgcttttc
    25321 aatcatttcc gcattatcag gagcttttac ttcttctaaa ttatattttc caacttctaa
    25381 atgattaacg atcaactggc ctttttcaat agatgcctcg gcaaagtcat tgttgccgat
    25441 gtcataagaa tgaccagtaa tgaaatgttt ggctttggtt tgatcagttg tccaagtgta
    25501 caatccatct gtgacacttt ggatgtattt tttgacgctt ggtgttcctt cttctttaga
    25561 aataataaat tctgctccat ttagtgcttc gttttcggca gtaccgattt ttgtaacttt
    25621 caacgttcca tcattaccga ctgtattttt agggtagaga tggatagtat ctagttcttc
    25681 tgtcccgtat ttataagagc catctgcttg tttgatcatc tcatatacag ggaaagctaa
    25741 aaccatgttt gcggcagctg acactccgtc ttttggttct tctttgatcg tgtagactgc
    25801 atctttccca ttttgttttt taggtaaaga taaagtgaca tttccatcag catctgtcgt
    25861 tcctgaagca actggtgtac caggagtcaa agactggact gcttgttttg ctgcatccac
    25921 ggacgctcct ttatctcgtt gcgcataaaa ttcttgagtg acgttataaa ctgaaaatga
    25981 aatatcggct aatccttggt attgatcgaa ttcgctcatt tctttcccgc tgttttggat
    26041 taaaggatcg ggtaaatcag tcattttctt tttatgaagg atgacttgag ctgctgtctc
    26101 ctctgcaaac actttttttg cgccgaagct gtttgtgagt aatggtaaaa taaggaaaag
    26161 gactcctaac ataacgttta tttttttatg atttttcatt ttttcactcc tgttcttttt
    26221 ttgataaaca accagattgc aaagcttgcg aataccatac caacaacgcc taaaagtgta
    26281 tttttcgttt cgtttgtttt tggaagaaat ggacggctat tttctttttt attgactact
    26341 ttcaatatac tgtccgcctc tttatcctca gaaagaaaag ttgaaatggt aaacggaatt
    26401 tctgtttcac ttaaaatata accagaaggt gcttcgatct cttttaatcg ataacttcct
    26461 gttttcaacc cggaaattga aaaagcgcca tttttatcgg aaatcaggtg aagcgcatct
    26521 gattcgttct ttgtccactt gtatccgttg gctgtttgct ggaggtaatt tcctgctttg
    26581 tttttaataa ggaatactgc gccagctaat cgtttatctg gtttatttgt ctcgattttg
    26641 acaaattgct tactccctgt atatacagag actttctttg tactgacacc aaaaccaaaa
    26701 tccagtgttc cgttgttgtt gatttccttg ttcgcctgtg ctgtactgct tagctgcatc
    26761 tgataagaaa tggtcagttt ttttccagca tatttttcca acgaagggat cgaaaataaa
    26821 acatcaaatc cgtgaggttt cttgtgtatg acaaagcctt ctgtcagctt ttctccattc
    26881 gatgaaatcg ttaaactatt aggtaaaaac gttaatgctt catccgcact atctgacaat
    26941 tcgaactttt gatagtcaag gatatttaca ggaattgtcg tatgcaagga ataagtgatt
    27001 ttttcaccaa tcgtaaaatc atttctaggc tcttctaata ccttctcaaa aggtggatca
    27061 gggtagtcgt tttcttcatt tttaggatac aaatggatag ttgaaagttt ttgattgttt
    27121 tgtccatgta caggaagaac aactaccata ttctctgcct tttctttgac gacttctggt
    27181 actttggatt caatgaaaag atacgcttta tctctttttt cttgatcttt agcggccaga
    27241 gaaaaagaag cgataccatc ttcgttgtta agagttgtag ttgttgcttc tgcggtaaac
    27301 ataccggaag acgcaccgtt ttttgcgatc tctgcttgtg cttcttctac cgttttgccc
    27361 ttttcccgta gatggtaaaa agaatctgtg acatcataaa cttggaatgt gacaccattt
    27421 aatcctcgat acgtttgtaa taaagctttt tcttgtccgt catttggatg atttttcggc
    27481 agttgcccgt ttgggaaaag caatttgtgt aaagtaatct gaaccatttc tgtttctgtt
    27541 tctgttgcta cctgagtaaa agctggttta aatagtaaca agaagagaca catactaagc
    27601 caaccaagtt ttttcatatt tagctgcccc ctctttcttt tttcaaaaaa agataccaaa
    27661 tagaaaacgc acaaccaatc atccctacta gatagattcc taaacggcca attcctcccg
    27721 tttcaggtaa tggtactttt gcctgattcg tgatgtctaa agaaatctgg ttgtttttgg
    27781 ctcctggtga cagaacagat tcatgatctt gtccatccac ttgaattgac ccatcttcgt
    27841 gtataactat agtaactggc tcttttagac cttggtatcc ttctggtgta aaagtttcgg
    27901 tcagcgtata agttccaggt tttaaatttt cgaatagaaa ggtatctgtt tcttgtccat
    27961 cttttggcga ttctaagtcc atttctggtc cctgcagtct gaattttgct ccttttaatg
    28021 tctgtccgtt gtcttctttt ttgtgtaccg ttagatcaaa cgcttttagt cgattttgat
    28081 gagtgagcgt ccatacatta tttgtcatct cggtattttt tcccgtgtaa atgatttttc
    28141 catatttatc gatttggaat tcataatttc ccggcgaagt atcatatccg gcaggaccag
    28201 ctgtttcaac gactcgatat ttaccaggtg tgagtgaatc aaaactggca agaccttttt
    28261 cattcgttgt ttggctgtca attggctgat aagtaccatt tgcttctttt ttctgtaggg
    28321 aaaatgctgc tcctttaaga tttatctctt tgtccgtccc ttgcatcgca tacttacgta
    28381 ctgctacagg aacttcaaca aaaggatttt caatagtcaa ctggatcgta tcatcggaag
    28441 tagtgactgt ttttccatca atggttaccg taccatcaga agcgatcttg atttcccaag
    28501 tagtcttttt gcttaatcca tgcccttctg gagcttttgt ttctgtcagc gtataggtca
    28561 tttctttttg caatttgaca ttttctggaa gagaataggt tccgtcgcca tgatctgtta
    28621 gtaaagtatc aatagaatct cctgttaatt tgaaaacagc gccaataaga tccttttcac
    28681 ctgtagagga atttttcgtt attttcaagt ttaacggtgt gaattgttta gtatttttcc
    28741 atgtcattgc atctatttgt tgagaatcgt atcctggtac aggtaattct ttgattgttt
    28801 ggtaactgaa tgcttgacct tgattattgt attgaggtag tgataatatc tcttgataac
    28861 tttctccgct atttgcagat aatttgtcaa tgtctgcacg ttcccacgta tttgttgtat
    28921 ctttagctgg tttaatgatt cgaatatatc cgtttttcca agctgcagca tttgtcgtat
    28981 gttcccgttg aatctcaaaa gtaacttgat ctggacgatc agctagattg ttgtcaaatt
    29041 cttcccataa ctttttgatg tgaagactga ctccgggagc tttagcagat ggtataccga
    29101 attcagctaa ttcattggta tcaatgtttg gctggaaagt tgtcctgccg ttcatttgat
    29161 accagaaatt tggatggaag tcctcatttt ctgtttggat tctcacttgg taatggattt
    29221 ggatttcttg gtcttttcct aaatagatct gattgctctt gatggtattt ccttctatgg
    29281 aaatagatgg agtgaccgtt gtaggacttg tccccacact cttgactgaa agtgtaccag
    29341 gctgataaat gaatggttct gcgattggat catttatttg tccattgctt acagttgctg
    29401 agatctgtac agcttttttg gctaggtatt cagagatatc tgttgcatga tcagctgatt
    29461 catagtaaag atcgcctttt tcatctgatg aaaccatttg tcgcatacga gactctactt
    29521 ctgcttttga gagaccagct gccggatcgc tttgaagttg gatgccaaga ccatgtattt
    29581 cgattcctcg ttctttgagt gccatcgctt ctccgatggt tgcgataaac gtactgtcga
    29641 ttcttctgga tagattgttt tggtcaggtg catcatagat tcttgagatt agagaagtat
    29701 ttcccggccg atcttgcgta ttagaaaact gagttccgta ataattgctg cttgattgtg
    29761 cgtgtacccg ctgtacttta taggaaaatg ttggtacacc atccgtcagc aaaacgatca
    29821 cttttttatg tccatttgga acggatagca tgcttcctgc atctcttagt gctttttgtg
    29881 taaaagtacc accatttgtc cgtgaaggtg taatggattt tacttgattt ttcactgaat
    29941 caaatgaacc catctgtact gcaccgttac tgtagctata tccttcgctt gagtagccga
    30001 catatcccat attgattttg tctgtgatac cgctatctgc taaagtatcg acaaaacgat
    30061 cgacaccaat ctttacttca ccgatccgat tattgtcgtt catacttcct gaccagtcta
    30121 cgaccaatac gagatcaaga ggcgtgatat ctttttgtac atttccacga gcattcaaat
    30181 aaacatcaaa caatccgggt gtacttgttt cctttgcata ctttcgtatc gcatagtctg
    30241 cttgattacc ggttcctcca tattcgatat acgaatgggt ccgatcatta ggatctccat
    30301 tccaagaatt tataccatcc cattgatttg ttccgttttt attgccttga tgatcaagaa
    30361 catttgtatt tcctgtaggt tgccaactgg ctttaggata agtgccctga tcatctgttg
    30421 tatattctgt aggaatcgtt tgattagatg gcacactcac agctggcaag gataatgtag
    30481 gcaatgtaaa tgggctgaca gaattttcgt tttcttttga tgattcttct tctatttcgt
    30541 ctggctttgg tactgacaca tctgtatctt tcttattttc tgtctcatca ataatgtcat
    30601 attctttatt atcgacgatc agcttgtttg cctggctgtg gtaaagtgct ggatcgattg
    30661 gaaaactcag atcgatagtt tgtttagtgt ttgatgagat ttccaacgta attttcccgc
    30721 tatttacctg ataagcatag ccatctcctt cttttctttc cacatcactt aagtaatttt
    30781 cgactagacc aatttctttt tctatctttt cagcactggt gttattcgca tttagcttga
    30841 tgttcagtgc tttttttcca gactgatccg aaatgacttc agctgaaatt gtttctaaaa
    30901 ctgttggatc atctgtagtg tctgctattg cagataatgg cacaaagttt gataaactca
    30961 atattatcag catgaaaata actttcagtt tcttccctgt ggtcgtcatt tttcccctcc
    31021 tttttctttt tttctgtttt gacgtgaata gtgaaaatta ttaaaataaa caggcgttac
    31081 ataattttat ttattttaat atgataatta ttatacgaag ggcgctaaag ggaatcacac
    31141 caattttcat accaattggt ttcatttatg aagttgattc cttcgattaa tgagcgtttc
    31201 attgaaagaa aagggttgag ggagaaaaac atatgatcag gttgttggaa ggaaactata
    31261 gaaagatgga aaaaatcatt cgaattctgt ccatttctga caagtggatg actaaaaaag
    31321 aacttgccaa acgaattggt agttcagaat ctacatttgt aaggtatatc gaagagatca
    31381 aaatccgctg gggaacggat gtaacgattc aatcttcaca aaaattgggg tatcgtcttg
    31441 aacatttgaa tgtaccagct tatttaaatg ttctgatgga aatgtcgcaa agttctacaa
    31501 acagtcgttt attaaatgag attattgtca atccgggtaa aacgattgag tattattgtg
    31561 aaaaaatttc catcagccgg tcttctttta cacgtaagtt gaaacaatgc aatcaaattt
    31621 tagaaagcta tttattaaaa gtgatcgttg accaaggttt tcagttaact agccttaaaa
    31681 gtgagctcca gttgcgtatc tttgttacct ttttcttttt aagttactac ggaagacgca
    31741 cgttgccatt tccaatcgaa aaaaagaaga tcaaagaaat tatggtcaag aataattgtc
    31801 agatgaattt gtttcaggaa gaaagcccat atgagcaatc tttctttatt atgtattttg
    31861 ttgtgcatct catgagagaa tcccaaggaa ctccatttga atggaaagct ggagactctt
    31921 atgtacagtt gataaatatc gataaaaaag acttcttaat cttgaaaaat ctcttaggaa
    31981 aattgaagta cagcagctat ataaaagtag tgaactattt tgtcaatacg attttttcag
    32041 aattttcgga gaaagaaaaa aatgaaatca gtgagcagat acgaaaaaat atgtcctact
    32101 gttcgttttc atccgctgat tgcaaagaaa aaaacgaaga atatgttgtt caattattaa
    32161 cgcaaattta ttttttctcc cgtattttcc catacgatac gactcatttg acgatccgtg
    32221 caacttactt tgcacagcag atgcaacacc gccagatacg tctatatgct ggatttcgtg
    32281 cagaacttca attttattcc actctactga atcagaatct gatttattat ttatctagct
    32341 atatcttttg gatgcttatc ggtaatcctg aaattcacca ttatacttct gataaaaaaa
    32401 tactgattat cagcgactta agtcttcgac atagtcaata tattgaagaa tatatcagtg
    32461 atattcttgc tgtgcataaa attcactctg agacaactgc tattactgaa gatcagctgt
    32521 cgaaatacaa tcttctcgag tatgatctga tcgttacgaa tcaacccatt ttgaatgccc
    32581 atgttcctca tatattgatt gatgatagtg tgtcattcgc aaatgaagaa gaactcatcc
    32641 gcttattcga gttatgaata atcaaaaaac agctatgtat aaaccaccct gataaaaatt
    32701 tcaggacaaa tacacatagc tgtttctttt tattgtattc cttcagcaat ccaacgagat
    32761 ttatagcgaa agtcttttaa caactcttct tttgtccaat tatttgcaat gagatccgtc
    32821 aaagtgttca ttacgagggt catggaaaag tataggaaac taactgcttc ttccgacgat
    32881 tgaatggaga aatgattgat tcttaataat tccagccatt tttcgttcat tggagaatca
    32941 gccgatagtt ctctacgttt ggttgtctgc tgatcatttc gctgggttaa caagtataac
    33001 ctttgccaat agatactttc gtgatctaag tcgcagcacc actctaaata tttttcgatc
    33061 cccaaaaaga aatgttgttt gttttcgcta atgaatttta agatatcttg atggactatg
    33121 tgagaactgc ttttgatcgt atacaaataa gcatcttcca aatcttgaaa atatttataa
    33181 aaagcaccgc gcgacatccc catcttgtca acgatttctg agactttgac ttggctgatg
    33241 tgctgtgagc aaaatatctc cagtaatagg tcatcgattc tctgtttctt tgcgttcgat
    33301 aaattgaaaa aagtttcttt tggcatccac atctctcctt tatctctgat tatacgtgga
    33361 tagcccttca ttgtcatgaa ggattcattc cttctttttc tcttcttctg tcaagtaccc
    33421 atcttccatt cgataaacgc gatcactcca ttgaatcata cggacatcat gagtaaccat
    33481 aatagtcgct ttttgtttct catgagcttc tttaactagt aatttgacga cttcatatgc
    33541 atgatctgta tccaaactag ctgttggttc gtctgctaag atcaagcttg gctcattata
    33601 taaagcgcga gcaattgcca ctctctgtct ttctccacca gaaaggtctt taggataact
    33661 atttttaagt gactggatat ctaaagattg cagcaaggaa tcgatttttg ttgaatcttc
    33721 tttttttgaa gtaacttggt caactagttc aaattgttcc tttaccgtta aaaaaggaat
    33781 caaattagag ctttggagaa taaatcctat ttctttaaag cgtaaatctg ctcgtttttt
    33841 ttctggaagc tcagttagtg agtgaccgtt gatcactatt tctccggaag tcggtgtttg
    33901 caatcctcct gaaatggtaa ggaaagtact tttacctgaa ccagaaggac caatgatgct
    33961 gacgaattct ccttccttta cataaaaatc aattgctttc aaagctgaga cctttgtatg
    34021 accagaacca aatgttttgg taactttttt catcgttaat atttttttca tttttattaa
    34081 cctcctattg ctgtaattgg gtctactttg gttatcgtac gtatcgaaaa caatccgcct
    34141 agcattgcga caatgatcaa aatcccacta tatagcaacc attgtgacca aaaaacagca
    34201 aaaggcatcg cgcttggtaa aatcagacta gtcaaataag caaacaaaag agcaagcaga
    34261 acgcctacca ccccaacaat aaaggactgt gctactaaag atttagcgat gaagaaattt
    34321 ttgatccctt gtgctttcat tactccaaaa atagcagtct tctgaagtgt catcacgtac
    34381 atgaaaatac cgaccacggc agcaaccact aagaacaaaa agtatatcat cgcatttaag
    34441 gtcatatttt gggcagaata gccaggaata ctttcaatga atgtcccaat agttaataac
    34501 tgtagaccct tcgatgcttt ttctttcgaa attttagctg aatcctttat tacgatccca
    34561 ttgatcggct gttcttcttc agaagcaaac ggctgttgtc catacttcaa agcagtccac
    34621 gtatctaggg acgcatagat gacgggagaa actgtgtaat aagtttcagg aaagataccg
    34681 acaatcgtca aaggatcgtc gtaactgcca attttgatcg tatctccaat tttatagcct
    34741 gaatcagcca gattttgaga gatgatgatt tcattctttg ttttaaattc atgccctttc
    34801 gtgagttttg gtaataaaaa tgcttctttc gttgtgccga aaactgtaac attttctttg
    34861 ttttttcctt tgattgctcc gctgtacaat ccgattggtg ctttctctcc tccttcaatg
    34921 tatttcagat ctcctcttgt taattgtgaa gctgcaaata cttgatttgc atcctccgac
    34981 aaaacaatct cttgtgcgtc ccaatcatcg attgcttttt taaactcttc tgaaagtcca
    35041 ttcgctagtc ctgacaacat gaatacgaca tacgcaatca agaacataat accgacgatc
    35101 aagccatatc gtaatttaga atagcgaatt tctttgatag ctagaaacat ttgtttttcc
    35161 tccctatgat agattgatag tgacaaaatg tcacttgttg aatagtgtaa ctctaaagtg
    35221 acaatttgtc acttgtgttg tttttttctt atcaataaat tgtctaagac atctttcgat
    35281 aaaaccttaa catgagtcat aaaaaattta tttaagcctt atttatgctt tccatgataa
    35341 actaaagtga ccaaatcaat gaggtgggaa aatgattaaa ctattagtag tagaagacga
    35401 taaagtatta tccaataata taaaagaaat actcgctgca atcggcgaga taacacaagt
    35461 ttatgatggt agtgaaggac tgttcgagtt ggaaagtgga gtttatgatc ttgcaatctt
    35521 ggatttgatg atgccggaaa tgaatggata tgaagtgtta gcaaaaatac ggaaagaaaa
    35581 aatacagact ccagtattga ttttgactgc aaaagatagt ttggaagata agattgaagg
    35641 atttgaacga ggtgcagatg attatctaac aaaacctttc tatcgagaag aactgatcat
    35701 gcgcgtgaaa gcattattga aacgctcgct aggtctcttt gatgaaaatg ttctgaagta
    35761 taaggatatc agttgtgatt taaaaagcaa tgaagtcttg tataaagaac aagttttgcc
    35821 catacaagga aaagagtttg aattgctcgt ttatttttta caaaataagg gaatcatttt
    35881 gacaaaagag cagatatttg accggatctg gggatttgat tctgatacga cgattacagt
    35941 tattgaagtt tatatgagca acttacgaaa acatctgcga agtactggat tggataaaga
    36001 aatcaaaacc ttacgtaatg tcggttatat actgcaaagg gtgtgatgca atgaatggag
    36061 atattacacg aaagcaacgt agtcgctttt ttgcgacaaa tgtagcagca tttgctttta
    36121 tcttttttct tttgggattc attactttac agatattaaa ccaatctgca tacagagaaa
    36181 cagatctgtc attagagaaa ataaattttg attctcgaac agtacaatta gagatacaaa
    36241 gatatcagca aggcgatccc tttttaaacc cgactcaaac ggaacaaaac agaacaaaca
    36301 tggaacagcc attaatggaa ccatcaaatg accgttttaa cactcaagtg atcttgtggt
    36361 ctaaaaatgg tgagatattg aataaagcag ctattggtgg gcgtttcaac caaatcaaag
    36421 aattaaaaat ggatacgaaa aatctgaata ccatccaaaa tattgaatta aatgaagaca
    36481 gtggctcatc tgccctctct ttccgttcga ttacgaaaaa agcggagacg aatcaagatg
    36541 gcgttgccta catccaaatt ttagaaaata caaatcaaat caagaattca ttagcgactt
    36601 ttcgcacgat tatgattttg tgtatgattg ttttttggat tctctctatt ggtatcagtt
    36661 attacttgtc tagtctaagc atgcgcccta ttattctttc ttggagaaga caaaaagaat
    36721 ttgttgagaa tgcttctcat gaattgcgca caccgttaac gattattcaa aatagtttgg
    36781 aacatctttt taccaagcct aatcatacta ttattgaaga atccgaaagc atcgctcaag
    36841 cattgtcaga gacgaggcgc ctcactggat tgacttcgga tttgctgaca atcgctcgga
    36901 atgattctga tcagcagctg ctttcaaagc aaatgatcaa tacacaagaa tatatcgaac
    36961 aacttgttaa accatttcaa gagatggcca tcatagatgg aaaagagttt attctccata
    37021 acgatgcaac attagaagta ccaattgatg aaaaaaaaat ccatcaagtt ttggttattt
    37081 tgctggataa tgcattaaaa tatacgaatc cagaagataa gatcattttg gattcatctg
    37141 ttaaccataa agaatggctg attcacgtaa agaatactgg cccttctatc agtgaagaag
    37201 atcaagcaag gatttttgat cgattttatc gggaagatca atctcgggcg aaagagactg
    37261 gcggatacgg gcttggcttg gcaattgcaa aacaggtaat cgaacagcat catggacaga
    37321 tttctgtatc caattatctt ccacaaggag tcgattttaa agtccaattg ccgttgaagc
    37381 ctaaaaaata atgcttatcc ctaaaaaagg gctgtgacaa aaatcttgtc acagcccttt
    37441 tttgttgcga ataaacggtg ttccagaagt agcttcttcg gaaataagcc gaaatttctt
    37501 taagctgacc acttctgtca caacctcttt agcttacgct tacgattttt tattgcggtc
    37561 ttcaatgtgt acatcaccgc ttgtgtcaat atccaattct tctcggctaa catcttctgt
    37621 cactttttca acatcttcat gttcttcttt atgaatccca acttcttcac gaataactgt
    37681 atgtttgctt acatctactt gctcttcttt gattgggata gtaatcgtgt catcttccaa
    37741 tttttcgtct gttccctgtg aagattgatc tgttactggt ttgcgttcga tgacgatttc
    37801 ttcacgtttg accggtactt ctaccgtttc agtatcgttg accacatgtt tgtggatgtc
    37861 gacttctcca gttgtcacat catgtgtatt tacatctaat ttttcttctt ttagttgaat
    37921 tgtatcatca tctagatttg gtttgcgatc gatgtcttca gtcttgcttg tacccattgt
    37981 atcagtctcg ccaggtacgt ccatcggatc agttcctggt gtcatattca atggatccgt
    38041 tgtattttga aagcctgtcg tgccacctac gtccattgga tctgttccag tgccagtttc
    38101 taatccagtc cccattgtgc ttgctgggct aacagtatct tgtgtatcca ttgtatcttc
    38161 tgttgtttct ggctgacgat agccttttac tgtgatgaca aattttccat cagaaatatc
    38221 tcctctatat tgatataaag gatcattttc tggtgtagcc gtctcagaat cataggtatc
    38281 aaaagaaaaa gtatctttta ttttttccca taaagaacgg tcttcttcac catccatccg
    38341 attttcatct gtttcaacat ctataccaga caatccgtca aatcctagtc tagatgctgc
    38401 ttcatcagta gtataaataa cgatatcatc tcgttcatac ccttcgttac gcaaccgttc
    38461 aattactgat aatgcttctt ccctcgtact atagcttccg atgaccgtta aatttttctc
    38521 ttccatgtaa attcctccta gcaaatagat ttatgattgg tatattgtta cttataaatt
    38581 tattctatag gagacgattg ttttctacaa atatttagat tggaatttga ttaaaacaat
    38641 ttaattgaaa tttgaaaaca tttatgttaa tgaaagtcat attctaataa aatgtcattt
    38701 gtttctatat gaatttttat gaaaaatttc atataatttc cactcgttgt gatggtttaa
    38761 tttatccaat ccatcaataa gcaaacaact tatataaaaa aggattaatt ctcaatataa
    38821 ttgctcctac catagaataa atcttcatgt aaagtaatga agtaccaact atttctaata
    38881 cttctttaag aattatccca acaatatcgt tagacaaacc attattgaat aaatggagag
    38941 ccaatcgatt ctgaaaaaat gttttctaat ttttcggaaa taaaagaaag agcctcctgc
    39001 cattagaacg aaccaaatcc atttcattgc tcatgaagta accacatagc taaaaagagg
    39061 ataaatcaat taaaagatca aaagaatgac tgcactaata aaataactaa aaatgtatct
    39121 tcttacttca tatcttttag ctagtactta gttaaaaatt tgcttcctaa gatcaagaca
    39181 aactgtctat cttcatttga tgaaatgcgg taaaatatag aaaatgaaca ctattggagg
    39241 gaatttgtaa tggacagcaa aactagaaaa caactgcaag aaaagttaaa gcaacgtaca
    39301 cacttgctcc cttccgtctt tcaacagttt ttagaagaaa atcgttcttc cctttcatta
    39361 ggcagtcgct atgaatacgc aaaagatttt catctgttta ctgattattt gagaaaagta
    39421 ttttctgaag aagtggatga tcagctgatt atccatttag aaaaacaaga ttatcagaat
    39481 ttcttagcta aaatctatcg acatactcgt tcgttccaaa ctacagcgaa ccaagaaacg
    39541 gagcaattgt ttgaaaatag ctattacggg atttcaagga agcaatattc attaaatcat
    39601 tttttacgat ttttgtatca aaaaggactg attgaagaag agatttctct tttgggtaca
    39661 agtttaccgg aaacaaccaa agcagctccg cgatatatcg atgcagaact tttcaaagat
    39721 tttgagcatt tgtttatccc gattgaaggc tttcaaacaa gcagagaagc tagctttgaa
    39781 gaaaagaata gaccgaggaa ccttgcaatt actgccattc ttctgtattg cggattaaag
    39841 atccatgaag tagtagaact taaacgtaaa gatgtatctt tgaccaagca gattcttaat
    39901 ctgaatagaa aagaaaacag actgaataaa gtaccaatac caaaagaagc gatgccattt
    39961 ctggagaact attttaagtt aacagcacaa aatacggacg aaaattcttt tgtttttcgt
    40021 tcctcacatg atcagcaaat ttctcctcgg actattcgcc aaattttgag caaaattaca
    40081 gtatataaaa acgtatcgcc agaactatgc agaaaaacat atcgttatta tgcagaacta
    40141 tatttagatg atgctgacgc ggtaaattat ttatgtggta atcgatacct ctcatcccat
    40201 tcttttcaag atatctggga aaagatggtt gatttttctt atcacgaatt agcggcattc
    40261 gttcgggttt cttccatata aaacaaatgt atgatctttt tttcacaata aactatgtta
    40321 ttatataaag gattatgtta cgattatctt gaatttgtat attacttaac aaaagataat
    40381 ggagtgtgaa tgatggagac cataacgtat tcccctttga acttatttac gaattttcaa
    40441 gaagctgcag ctaacttttc cgagaccccg attatctttg atgagccact ttcagctttc
    40501 ccagaattag gacttgaaac aacttacaaa aaatgctgcg aaagtgtgtt gaaacgggct
    40561 tatcaattgg ctcatcttgg agtgaaatcc ggagacaaag ttattattta taagagttcc
    40621 gcttttgaca cataccttct agctgtttct gtgtcttacc ttggtgcagt gcctgtcatg
    40681 acttcttatc atctaccaac aacaacaatg gaagtattta tcgatcgttt agaagaccct
    40741 ttcattttat ttgatgatga gacaaaaaag cgagtgagag aaatttcaaa tggaacaaaa
    40801 tcaaaacaga ttcc
//
LOCUS       DACJGB010000017        38225 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.017, whole genome shotgun sequence.
ACCESSION   DACJGB010000017 DACJGB010000000
VERSION     DACJGB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.017"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(441..1751)
                     /locus_tag="IYI34_000986"
     CDS             complement(441..1751)
                     /locus_tag="IYI34_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAR0558870.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1763..3181)
                     /locus_tag="IYI34_000987"
     CDS             complement(1763..3181)
                     /locus_tag="IYI34_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0558871.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3421..4989)
                     /locus_tag="IYI34_000988"
     CDS             complement(3421..4989)
                     /locus_tag="IYI34_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAR0558872.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4993..5403)
                     /locus_tag="IYI34_000989"
     CDS             complement(4993..5403)
                     /locus_tag="IYI34_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAR0558873.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5400..6323)
                     /locus_tag="IYI34_000990"
     CDS             complement(5400..6323)
                     /locus_tag="IYI34_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAR0558874.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6320..7198)
                     /locus_tag="IYI34_000991"
     CDS             complement(6320..7198)
                     /locus_tag="IYI34_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAR0558875.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7185..8522)
                     /locus_tag="IYI34_000992"
     CDS             complement(7185..8522)
                     /locus_tag="IYI34_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAR0558876.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8525..9628)
                     /locus_tag="IYI34_000993"
     CDS             complement(8525..9628)
                     /locus_tag="IYI34_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAR0558877.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9702..11210)
                     /locus_tag="IYI34_000994"
     CDS             complement(9702..11210)
                     /locus_tag="IYI34_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR0558878.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11176..11868)
                     /locus_tag="IYI34_000995"
     CDS             complement(11176..11868)
                     /locus_tag="IYI34_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAR0558879.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11890..12864)
                     /locus_tag="IYI34_000996"
     CDS             complement(11890..12864)
                     /locus_tag="IYI34_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0558880.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13164..14036
                     /locus_tag="IYI34_000997"
     CDS             13164..14036
                     /locus_tag="IYI34_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0558881.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14494..16692)
                     /locus_tag="IYI34_000998"
     CDS             complement(14494..16692)
                     /locus_tag="IYI34_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0558882.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16924..17916
                     /locus_tag="IYI34_000999"
     CDS             16924..17916
                     /locus_tag="IYI34_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0558883.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17976..19397
                     /locus_tag="IYI34_001000"
     CDS             17976..19397
                     /locus_tag="IYI34_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAR0558884.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19390..20889
                     /locus_tag="IYI34_001001"
     CDS             19390..20889
                     /locus_tag="IYI34_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAR0558885.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20928..22322
                     /gene="uxaC"
                     /locus_tag="IYI34_001002"
     CDS             20928..22322
                     /gene="uxaC"
                     /locus_tag="IYI34_001002"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAR0558886.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22481..23164
                     /locus_tag="IYI34_001003"
     CDS             22481..23164
                     /locus_tag="IYI34_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAR0558887.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23166..24002
                     /locus_tag="IYI34_001004"
     CDS             23166..24002
                     /locus_tag="IYI34_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAR0558888.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24013..25767
                     /locus_tag="IYI34_001005"
     CDS             24013..25767
                     /locus_tag="IYI34_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558889.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26043..26384)
                     /locus_tag="IYI34_001006"
     CDS             complement(26043..26384)
                     /locus_tag="IYI34_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAR0558890.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26452..28623)
                     /locus_tag="IYI34_001007"
     CDS             complement(26452..28623)
                     /locus_tag="IYI34_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAR0558891.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28834..29691
                     /locus_tag="IYI34_001008"
     CDS             28834..29691
                     /locus_tag="IYI34_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAR0558892.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29762..30619)
                     /locus_tag="IYI34_001009"
     CDS             complement(29762..30619)
                     /locus_tag="IYI34_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR0558893.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30604..33306)
                     /locus_tag="IYI34_001010"
     CDS             complement(30604..33306)
                     /locus_tag="IYI34_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAR0558894.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33319..34608)
                     /locus_tag="IYI34_001011"
     CDS             complement(33319..34608)
                     /locus_tag="IYI34_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAR0558895.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34758..35804
                     /locus_tag="IYI34_001012"
     CDS             34758..35804
                     /locus_tag="IYI34_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0558896.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35806..37959
                     /locus_tag="IYI34_001013"
     CDS             35806..37959
                     /locus_tag="IYI34_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAR0558897.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11280 a         7859 c         6799 g        12287 t
ORIGIN      
        1 ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt caaaggtcta
       61 cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt atatcatatc
      121 agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg tttttcgaaa
      181 tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa cttttttgaa
      241 gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta gttatcttat
      301 cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt tcaatgaagc
      361 gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa atattattta
      421 agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca
      481 gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc gattcgcccg
      541 aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg cttttcagga
      601 aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag gcatgcctgt
      661 acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg tatttcttca
      721 gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt tctattgtat
      781 tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat attcagattt
      841 tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat tcgaatacca
      901 cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc agcactaact
      961 tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac ctcgattctt
     1021 gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat agcaatacaa
     1081 tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga atcaatatca
     1141 atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac taatacatcg
     1201 ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca
     1261 acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc ataatccgca
     1321 atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg atcttttccc
     1381 caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc ttctataaga
     1441 atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc tacgccggct
     1501 actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag gactgcacct
     1561 tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa ataaatgcct
     1621 tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt ttgcagtatt
     1681 attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat aatccctttt
     1741 tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct tcaccaataa
     1801 atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg gatacaaata
     1861 agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact aaaactgatt
     1921 ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata aatccgccta
     1981 ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca tatactgttc
     2041 catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta atgaatgccc
     2101 agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga atcagatata
     2161 cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa gaagcaagta
     2221 acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta gcaaatggtg
     2281 ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct tgtttattat
     2341 ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga ttgatcacaa
     2401 tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa ccttttacca
     2461 actgagaagc agatactttt tcgtttttga caactcttac tctttcagta gtcaatttac
     2521 aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc caaaagaaat
     2581 gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa atcggaatga
     2641 agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg
     2701 tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg atattgattg
     2761 ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca
     2821 ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa aatggatact
     2881 tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag acatcacata
     2941 atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag aataacgtac
     3001 ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag aaactattga
     3061 ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc ttatcggaaa
     3121 tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt tgtgaaagca
     3181 tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct ttttttcttg
     3241 tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa aaacaaaaaa
     3301 taatctaaaa acattgatgc ataagattga taactttttt tacataacaa tatatacaaa
     3361 caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta aagaattaaa
     3421 ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac ttcctctttt
     3481 tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc ctgagagtat
     3541 acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt ctttcaaatg
     3601 aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg
     3661 aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac gtaggcattt
     3721 tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat ttaagtacag
     3781 taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg tctccgcatc
     3841 aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag attgaagaga
     3901 ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc aatcagcatt
     3961 tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg tatccataaa
     4021 ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt cttccatcgg
     4081 agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca cttcttgaat
     4141 agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa gatatgtcat
     4201 gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt gcaatgtaga
     4261 agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt aaggaccagt
     4321 aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc cttctgccgt
     4381 catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt tcgctaattc
     4441 tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg catcatattc
     4501 ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc atagttcatt
     4561 gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa agccgttact
     4621 atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga tcaaataatt
     4681 gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa gataaaacaa
     4741 tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat ttccttgcaa
     4801 agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat ttttcgactc
     4861 atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat accgctcttt
     4921 cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt tcactttgat
     4981 agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga atgccccagc
     5041 tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca acaatatgaa
     5101 tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag cagtcaaaaa
     5161 caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca gaccagtcat
     5221 gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta cagcaagcga
     5281 ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg ctaacccgcc
     5341 aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc attttctttt
     5401 catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat cgatgtttca
     5461 taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc atcccaatta
     5521 tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa gacatttgca
     5581 tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc agtcaaggta
     5641 cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc tgtgatgtat
     5701 ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc atctgcccca
     5761 ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg acgacaatac
     5821 tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac
     5881 ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa agatacgcga
     5941 tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc tgctgtattt
     6001 tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt agcacttcgg
     6061 aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt accggagata
     6121 actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat atattcatca
     6181 tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc tgccaaataa
     6241 tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt
     6301 atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa atatttctca
     6361 tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt tctggactac
     6421 ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct tcgtccaagc
     6481 acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt ttttcttgga
     6541 ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac ccatgatgat
     6601 ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga tcaatacaaa
     6661 ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc atagaaagac
     6721 caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc
     6781 tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa aattgtttga
     6841 acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa
     6901 cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag taaggagcac
     6961 catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga
     7021 ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact gtttccaatt
     7081 catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct cggtataatc
     7141 gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc aactgcataa
     7201 gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga ttggaatttc
     7261 tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg attttcaatc
     7321 gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg tctgctcatt
     7381 tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc catcgagaca
     7441 tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata aataaaacct
     7501 gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct gaatgctggt
     7561 gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt ccctcgcgga
     7621 ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat gactatattg
     7681 gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa tcgtattcct
     7741 cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact accactcatt
     7801 tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt gatcgtgata
     7861 ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc aatacagtca
     7921 tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc tggatcaatt
     7981 ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa cgttgcatta
     8041 ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat atctttcaca
     8101 gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc gagattatct
     8161 ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt accgaaacca
     8221 gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac ttttcgatgg
     8281 actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga gaatttcaga
     8341 accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc tcctgttaga
     8401 aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc atcaatggct
     8461 tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa aatatcatac
     8521 atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat
     8581 cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc tgatctgctc
     8641 cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct tttgtttcca
     8701 gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc cattcttctt
     8761 tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata ctactgccgg
     8821 aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac catgtcccgc
     8881 tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt tctttcagca
     8941 aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc atcaaatacc
     9001 agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca gcccatggtt
     9061 ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg aatagatgtt
     9121 tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca ctaaaattct
     9181 ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa atttgataag
     9241 gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt gccaactgct
     9301 gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg tacaataaaa
     9361 agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg tagccacgga
     9421 tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca aaataaattt
     9481 tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca tagtcataag
     9541 accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc atgatacttt
     9601 ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt aaaataggat
     9661 ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc cgctgctttg
     9721 tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca gtcttctaca
     9781 gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt agcatcaatg
     9841 acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa
     9901 ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa ttctttttca
     9961 gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga taacggttgc
    10021 acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc attcaattca
    10081 taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc taaaagatca
    10141 tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga tttgggaaat
    10201 gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc gccttcacca
    10261 tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg acccatcgtc
    10321 ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg tgcaaattgt
    10381 tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc attctcaaat
    10441 agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc tggagtgaat
    10501 ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt gaaaagttta
    10561 aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt gtcgtccaca
    10621 ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag ctcatccatg
    10681 gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac taagccagca
    10741 cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact cgcagcttcg
    10801 attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc tgatacatcc
    10861 cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat cgcaatttga
    10921 agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct ttcttctttt
    10981 gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc
    11041 acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt gattttagct
    11101 tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc ggcaagtgct
    11161 agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat aaattttctc
    11221 cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg agttcttcca
    11281 ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga agagactgcc
    11341 accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa tccctaccat
    11401 acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa atgccatttt
    11461 cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa tatagagtgc
    11521 tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc aattgtgtat
    11581 gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc tcatcagcaa
    11641 ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc
    11701 ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag gaccgaaagt
    11761 aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat aaaccaactg
    11821 taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat ctttccctct
    11881 ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat tagctaaaat
    11941 taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga aacttaactg
    12001 tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt
    12061 caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct gaccgatttt
    12121 tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat gccatgtttt
    12181 gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat cgactgcagt
    12241 tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca cttgtccgat
    12301 atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa atcccattgt
    12361 tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag taatcgaaac
    12421 aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa
    12481 tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg gaaaaaacac
    12541 gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga atgtcggttc
    12601 tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta ttcctaaaaa
    12661 tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc taaaaatcaa
    12721 gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt tgattcctgc
    12781 aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca gttgaaatgg
    12841 ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt ttgctgctta
    12901 tgacttcatg atagataact atctgaatta cgtatataat catttttacc gtaagtaaaa
    12961 acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa
    13021 taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat aggaaatgat
    13081 tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa catttaattt
    13141 attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa agaagtttta
    13201 cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt ccttttttaa
    13261 atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag tttatccttt
    13321 ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg accattttta
    13381 gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa
    13441 cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact
    13501 ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc ttagttgttc
    13561 gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct gttaatgcaa
    13621 tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg gaagaatcag
    13681 caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg ccattagcgt
    13741 taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac acttatatga
    13801 ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt ttacgccaaa
    13861 tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg attccaccag
    13921 cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg attgtttatc
    13981 cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat
    14041 gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa taagaaggca
    14101 tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg aagaatctac
    14161 tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag cgatgataaa
    14221 aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa
    14281 tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc ttgaacgcca
    14341 tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg cgtgcttagc
    14401 cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc ttttattgct
    14461 tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc gatattctcc
    14521 cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat ttcgatattg
    14581 caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt tttttgctac
    14641 ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct ttttaaatac
    14701 attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta aagacagatc
    14761 acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt taaattcatg
    14821 atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta aaacaagttt
    14881 ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt tcatgttatc
    14941 aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat tgatcaagcg
    15001 gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg catcaatcaa
    15061 ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa acaatcgatt
    15121 ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa cgatttcctg
    15181 ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt cttttgcccg
    15241 atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc ctatactgat
    15301 agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact ccatgacttt
    15361 tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca tttcttcatt
    15421 caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca tgttgttgat
    15481 cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat cgatttgaac
    15541 caaagctgta taatagcagt cattttgaga actataacca aattgctgca accgctgatc
    15601 tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg tttccagctg
    15661 tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga tccgactgac
    15721 tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa gattcagacg
    15781 ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg aaagaagacc
    15841 aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat tgcggatcat
    15901 tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat aatctgattt
    15961 cagtactata tacgttttat ctttttctct tatcactttc agctgattgt aatctagatc
    16021 cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag taaaaaggtt
    16081 gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa cttgttctat
    16141 tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa acattggcaa
    16201 agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat ttttgttatt
    16261 tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca acgatccatt
    16321 gattactccc catttaccag ccaaactgac taaatcataa ttattatcat ccataacaat
    16381 cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat ctgtatatgt
    16441 cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata
    16501 ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca aattcttttt
    16561 gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga ataaactaaa
    16621 tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat aaaatactgt
    16681 acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat ttttctgtaa
    16741 aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca taattaaaaa
    16801 taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc tattgactta
    16861 tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat
    16921 ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac gacagtttct
    16981 cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat acgacaacta
    17041 gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa
    17101 tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa tttcctagca
    17161 gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc tgttgaaagt
    17221 atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact gatcgttatg
    17281 agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga atttcctttc
    17341 gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc cgatgatgcg
    17401 gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa gatcgcttat
    17461 attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt
    17521 ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga ttacagctta
    17581 gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc tactctggtt
    17641 ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt agctggcctc
    17701 cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc
    17761 aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag
    17821 cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga gctctccacc
    17881 ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt tatataaaaa
    17941 tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca aaagaattag
    18001 tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg
    18061 gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa ggtaatgttg
    18121 caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa gataatttat
    18181 atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc gagattatca
    18241 ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta tctctagcag
    18301 aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc caattcgtgg
    18361 agactgataa tttaacagat actcccccac agagcttccc gggcaaactg acagcctttt
    18421 tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc gaactgattg
    18481 acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa tgggatttag
    18541 aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc
    18601 gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg cataactatg
    18661 aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag
    18721 aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc acagatgata
    18781 tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca gcgatggtcc
    18841 ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat cctttattct
    18901 ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct ttacctaaag
    18961 atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct tttgcccatc
    19021 acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag
    19081 tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact gtttcactag
    19141 ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat gaaaagacga
    19201 taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga ttattgcaaa
    19261 acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat acagttcaag
    19321 aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa atgaaaggaa
    19381 cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg agacagtgtg
    19441 gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca gcaaacgatc
    19501 accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt gaaagctgga
    19561 gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt aactgccggt
    19621 caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga ttatcgatat
    19681 gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca aggttacttg
    19741 cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac cgttgggtgt
    19801 gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc agatctggga
    19861 cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct tggaaaagat
    19921 catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc cggaggagtt
    19981 ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa aattttaggt
    20041 gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga tgagatcgaa
    20101 caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca gcgcatatca
    20161 gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg tctatcggga
    20221 ataactgcta atcctttact tggtgcattt tctgactact tgattgctca aggaggcagc
    20281 acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat ggcacgagcg
    20341 gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa acagtatttc
    20401 ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc aggtgggatc
    20461 actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt
    20521 gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt gcaagctccc
    20581 gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt ggtcttgttt
    20641 accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt atctacgaat
    20701 acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt cttattagaa
    20761 caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga
    20821 gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa aaacggagta
    20881 acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg tttttatctg
    20941 atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac gcaaaaaaca
    21001 tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa aacaacaatt
    21061 tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc ttgatgcgag
    21121 cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa aaatttttag
    21181 cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg tcacatcttg
    21241 aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca gagaaaattt
    21301 ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa
    21361 aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg cactatcatc
    21421 agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt ccagataaag
    21481 cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa gaagcagctg
    21541 accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg atagaatact
    21601 tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc tataaagaag
    21661 cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa ctattatctt
    21721 cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac ttctatcatt
    21781 ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat acacaaatgt
    21841 tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct ttgactattc
    21901 ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag acgatccttt
    21961 attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt ttccaaaaag
    22021 aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact cgagctggta
    22081 tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc
    22141 tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt cgtgttcttt
    22201 gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa caacttttag
    22261 gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc ttttttgcct
    22321 aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac ggaatccgtt
    22381 tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct cttgctgttc
    22441 tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta tccatattgc
    22501 cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag gttggggaga
    22561 aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa agaatggacg
    22621 cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac agtttcagcc
    22681 gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag aagaaagagg
    22741 tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa
    22801 agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact tagaaaacgg
    22861 aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa
    22921 acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc agaccttctc
    22981 aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta aaaattaccc
    23041 agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag ttgcccgaat
    23101 aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac aaaaaggagt
    23161 ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg
    23221 aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt gcaccacaaa
    23281 aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct gcctattttg
    23341 gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat atcaacctat
    23401 ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag caaattgctc
    23461 tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc tctgtcggtt
    23521 acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct atcattgaat
    23581 tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc
    23641 caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca ccaaatttaa
    23701 aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat caggaaaaag
    23761 tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat ctcaaagact
    23821 atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt ttagatttcc
    23881 gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact cttgaagcta
    23941 ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac caagattgtt
    24001 aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca tgcaaatgct
    24061 tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat gtcaggactc
    24121 ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc caatgtaatc
    24181 aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa cgcttttttg
    24241 atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa ataccatagc
    24301 acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa aatccaagaa
    24361 ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa gatcatccgt
    24421 gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt aaatatcgct
    24481 gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt tttcatgttt
    24541 ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa aaggatcaga
    24601 cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg
    24661 atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac aactcgctta
    24721 aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct gcaatctgtt
    24781 ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt gattttcact
    24841 gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat
    24901 ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac gatttcaaaa
    24961 ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt agaaacttat
    25021 cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga tgtttctttt
    25081 ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt gaaagctgga
    25141 gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct taacttattg
    25201 attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc tttggatgaa
    25261 ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac tttgttgttc
    25321 tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa agaacgttta
    25381 gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc tgatggtctg
    25441 gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca acgtatctcc
    25501 attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc aacctcagct
    25561 ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac agaaacaccc
    25621 actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa aatcgttgtg
    25681 atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc tcaaaaaggc
    25741 tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag acttttgtcg
    25801 tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    25861 agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag agttaaacgg
    25921 taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca ataagaagca
    25981 attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt cataaaatag
    26041 ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa gtcattgatg
    26101 attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc tgattggatt
    26161 ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg tccactcatt
    26221 tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct
    26281 tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg taattcacgg
    26341 atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg ccctcttttc
    26401 ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg gctactctcc
    26461 ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc cttctttcac
    26521 catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat tcaagttatc
    26581 ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat ctgcagctac
    26641 cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg aagtatacgg
    26701 aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac ttgtcccttc
    26761 caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca ctacgtctgg
    26821 cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag tttctgtggt
    26881 tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg ttccatttga
    26941 aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata tgacctgttt
    27001 tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga ttttcgtgat
    27061 aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat
    27121 caatggagat actccttttt ccagtccacc aagggctaag ccataatact tatcagattt
    27181 tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac cgatctcatg
    27241 caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca tcggtacttc
    27301 tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa
    27361 gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta atggtttgat
    27421 cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa agacatgatc
    27481 tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag ctactgaagc
    27541 agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt tttgataagt
    27601 agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat agcctttatt
    27661 catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca ctgcatcaaa
    27721 ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa gatacgcgct
    27781 gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt tttcttccat
    27841 tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga tcggattata
    27901 aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg taacattcgt
    27961 tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc cgttgccaaa
    28021 ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct tttcgatctc
    28081 gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc
    28141 atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc cgccagacaa
    28201 gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat gctcgtaaaa
    28261 attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg cgtttaattc
    28321 tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct tatcataaat
    28381 gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat
    28441 aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt
    28501 cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga gatatggtct
    28561 gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc
    28621 catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata gtgaaagagt
    28681 gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc aagtagagta
    28741 acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa aatggtacaa
    28801 ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac tttacctatg
    28861 aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt tcctagaaag
    28921 gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc tgctttgttc
    28981 catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac agattataat
    29041 tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga agatgagcat
    29101 ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc gacagaaaac
    29161 agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg accttacgtt
    29221 gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc
    29281 ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata tcaagccatc
    29341 gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg agatcgccat
    29401 gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac acatgtcact
    29461 gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac cggacgtaca
    29521 caccagatca gagttcattt agctcataag ggacatccga tcgttggaga ccctctctac
    29581 caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat gatccatcca
    29641 tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata gaaataaaaa
    29701 agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt aatttgtctg
    29761 cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca ctttcattcg
    29821 gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa ccacccatca
    29881 attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt accccaccgc
    29941 ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct tgagcaatat
    30001 aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct ttgatccctg
    30061 tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta tgataaggac
    30121 aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc atttcaggat
    30181 gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt cctgttccca
    30241 cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat tccccatatg
    30301 cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc ttgattgtgc
    30361 caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat ccgtaattat
    30421 cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc ttcaattcat
    30481 aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg gtagggatac
    30541 ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt gttccgcctg
    30601 cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa tctcataagg
    30661 ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca atatgacagt
    30721 ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat tgaaacctct
    30781 tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa acgtttcact
    30841 ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt tgatttgttg
    30901 agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt tttcttctgg
    30961 tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg cttctggtgt
    31021 tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca ctgcgatttg
    31081 tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta caccgtactc
    31141 ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt tttcccaact
    31201 tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt cgtgatgttc
    31261 cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct gattgtcgag
    31321 agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg tttttatttg
    31381 aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca caaccatctg
    31441 ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct gtagaatact
    31501 tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt ctttgttact
    31561 ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc ctatatcgcc
    31621 gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat cttccattgt
    31681 aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga ttcgtccact
    31741 ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa gcgcgaatgt
    31801 ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca ttcttgccat
    31861 atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag tttcttcaga
    31921 taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac gtttaggcaa
    31981 attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt ttctttctaa
    32041 ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta caatgacaaa
    32101 tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct gttctaagga
    32161 atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta tcatttcccg
    32221 atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt gcattaatgt
    32281 cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat aagccatcgt
    32341 cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc attgttttaa
    32401 gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt cttgacttgt
    32461 caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg cttccagata
    32521 gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg cgagatggat
    32581 tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca tcaataagtg
    32641 gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg caagagctgc
    32701 ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata gcagaccaaa
    32761 gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag aatagttttc
    32821 tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa acgctgctgc
    32881 tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa atgtatcggg
    32941 aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc caatcagcat
    33001 atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat aaaatggtcc
    33061 aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt ctggccttac
    33121 ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt tgaaggacgg
    33181 atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca tgtgatgctg
    33241 ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat atactttctt
    33301 tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca tgactaattc
    33361 gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag gattgttcac
    33421 atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta aattcaatat
    33481 ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg caatcggcca
    33541 aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc cttcatagaa
    33601 atatggattt tctttactca acaatgttct tcttgtttgc agataatctt cctcttgttc
    33661 agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt catccatgac
    33721 tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt tttgattaac
    33781 cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct gtgtttgctg
    33841 tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac ctaacacgac
    33901 caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat cgctaggacg
    33961 gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt cattgatcag
    34021 cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt catgatcttg
    34081 ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt gattgaattg
    34141 atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt agcataaaga
    34201 atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat ctgtttggtg
    34261 gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat tgatataaaa
    34321 aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt cttttgcaat
    34381 cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg gaatgtctcc
    34441 cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca gagtattggc
    34501 aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt tttgtgtgat
    34561 atcttccata aatttctgca ccgattttgg tatatcttta taagccacta ataaaacctt
    34621 ctttctattt gatatatctt taaagattat acttcataca gccaattatg tctacgcttt
    34681 caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa tatgatatat
    34741 cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat gatttgcaaa
    34801 cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa aaagaacttt
    34861 cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa ttagagcaat
    34921 taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct tctccggaac
    34981 agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca tatgtcgctg
    35041 atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat aaacagattg
    35101 atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag ctaatcaatg
    35161 aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac ttactttttg
    35221 agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat gagttacctt
    35281 ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct tatttgatcg
    35341 aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca caatccgttc
    35401 gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact ttccacactt
    35461 cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact aagtatgaag
    35521 taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat ctcttgagac
    35581 agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat atccaagcag
    35641 cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag ttaggtaagc
    35701 ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc gatagtaaat
    35761 ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa tatcggtcaa
    35821 atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa ctgccagcct
    35881 tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga tcaaaacggc
    35941 agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac ccatcaacca
    36001 agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg aaaactacag
    36061 gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat attttgcccg
    36121 actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat acctagcatg
    36181 tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact tttatccttt
    36241 ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa aatcacacat
    36301 tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt tgaatatcct
    36361 ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc gttgtatttt
    36421 gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc ggaacaagca
    36481 gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc tcacagtcaa
    36541 gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca acaacaattg
    36601 cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt ttatgaaatt
    36661 gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa acctggtgtt
    36721 ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct ttattctttg
    36781 attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta tagagaaagt
    36841 ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg gacattgatc
    36901 gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat gcctgaattt
    36961 ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta tggacgccaa
    37021 ggaacagaag attacttgac atatggctat gttcctaatc actaccacga atcagtcaat
    37081 cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa ggtactagaa
    37141 gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa tatctttgac
    37201 tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga accattttca
    37261 gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag ctttgctgtt
    37321 tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata taaagaaaaa
    37381 ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg ttttgagatc
    37441 cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc aaaccagcca
    37501 agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc acagccactg
    37561 cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc tggagatgag
    37621 gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta tcctgtcacg
    37681 cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt tttgcttttg
    37741 tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca acaatttatc
    37801 cataaaatca aacggatgga caaagaatat acgaaattat attttaccca tcaagatttg
    37861 ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa gaaatatcaa
    37921 gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt gaaaaagctg
    37981 tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt ttcgtttggt
    38041 tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt tgtgaaatca
    38101 gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata ttttgccatt
    38161 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag gtcgtgaaaa
    38221 agctg
//
LOCUS       DACJGB010000018        37041 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.018, whole genome shotgun sequence.
ACCESSION   DACJGB010000018 DACJGB010000000
VERSION     DACJGB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.018"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            2..74
                     /locus_tag="IYI34_001014"
     tRNA            2..74
                     /locus_tag="IYI34_001014"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IYI34_001015"
     tRNA            78..150
                     /locus_tag="IYI34_001015"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IYI34_001016"
     tRNA            155..225
                     /locus_tag="IYI34_001016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IYI34_001017"
     tRNA            238..311
                     /locus_tag="IYI34_001017"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IYI34_001018"
     tRNA            323..394
                     /locus_tag="IYI34_001018"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IYI34_001019"
     CDS             660..1853
                     /locus_tag="IYI34_001019"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAR0558898.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IYI34_001020"
     CDS             1983..3455
                     /locus_tag="IYI34_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0558899.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IYI34_001021"
     CDS             3538..4326
                     /locus_tag="IYI34_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAR0558900.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IYI34_001022"
     CDS             complement(4393..5061)
                     /locus_tag="IYI34_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAR0558901.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IYI34_001023"
     CDS             5176..6888
                     /locus_tag="IYI34_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558902.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IYI34_001024"
     CDS             6891..8663
                     /locus_tag="IYI34_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0558903.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..9866)
                     /locus_tag="IYI34_001025"
     CDS             complement(8745..9866)
                     /locus_tag="IYI34_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0558904.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(9938..10870)
                     /locus_tag="IYI34_001026"
     CDS             complement(9938..10870)
                     /locus_tag="IYI34_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAR0558905.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(11309..11761)
                     /locus_tag="IYI34_001027"
     CDS             complement(11309..11761)
                     /locus_tag="IYI34_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAR0558906.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(11774..12184)
                     /locus_tag="IYI34_001028"
     CDS             complement(11774..12184)
                     /locus_tag="IYI34_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0558907.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            12459..12671
                     /locus_tag="IYI34_001029"
     CDS             12459..12671
                     /locus_tag="IYI34_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0558908.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            12674..12886
                     /locus_tag="IYI34_001030"
     CDS             12674..12886
                     /locus_tag="IYI34_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558909.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            complement(13178..13402)
                     /locus_tag="IYI34_001031"
     CDS             complement(13178..13402)
                     /locus_tag="IYI34_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004401476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAR0558910.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            13480..13668
                     /locus_tag="IYI34_001032"
     CDS             13480..13668
                     /locus_tag="IYI34_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558911.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            13994..14329
                     /locus_tag="IYI34_001033"
     CDS             13994..14329
                     /locus_tag="IYI34_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558912.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            14562..15503
                     /locus_tag="IYI34_001034"
     CDS             14562..15503
                     /locus_tag="IYI34_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAR0558913.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            15505..16395
                     /locus_tag="IYI34_001035"
     CDS             15505..16395
                     /locus_tag="IYI34_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HAR0558914.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            16438..17238
                     /locus_tag="IYI34_001036"
     CDS             16438..17238
                     /locus_tag="IYI34_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAR0558915.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            17253..18104
                     /locus_tag="IYI34_001037"
     CDS             17253..18104
                     /locus_tag="IYI34_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR0558916.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            18305..18766
                     /locus_tag="IYI34_001038"
     CDS             18305..18766
                     /locus_tag="IYI34_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0558917.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            18792..19244
                     /locus_tag="IYI34_001039"
     CDS             18792..19244
                     /locus_tag="IYI34_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558918.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            19350..19544
                     /locus_tag="IYI34_001040"
     CDS             19350..19544
                     /locus_tag="IYI34_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558919.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            19575..19868
                     /locus_tag="IYI34_001041"
     CDS             19575..19868
                     /locus_tag="IYI34_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558920.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            19898..20338
                     /locus_tag="IYI34_001042"
     CDS             19898..20338
                     /locus_tag="IYI34_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0558921.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            20413..20880
                     /locus_tag="IYI34_001043"
     CDS             20413..20880
                     /locus_tag="IYI34_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAR0558922.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            21627..21857
                     /locus_tag="IYI34_001044"
     CDS             21627..21857
                     /locus_tag="IYI34_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558923.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            22016..22849
                     /locus_tag="IYI34_001045"
     CDS             22016..22849
                     /locus_tag="IYI34_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HAR0558924.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            22842..24257
                     /locus_tag="IYI34_001046"
     CDS             22842..24257
                     /locus_tag="IYI34_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAR0558925.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            24269..25798
                     /locus_tag="IYI34_001047"
     CDS             24269..25798
                     /locus_tag="IYI34_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAR0558926.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            25890..26768
                     /locus_tag="IYI34_001048"
     CDS             25890..26768
                     /locus_tag="IYI34_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAR0558927.1"
                     /translation="MMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            26770..27087
                     /locus_tag="IYI34_001049"
     CDS             26770..27087
                     /locus_tag="IYI34_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558928.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            27136..27837
                     /locus_tag="IYI34_001050"
     CDS             27136..27837
                     /locus_tag="IYI34_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAR0558929.1"
                     /translation="MVEWWGATIKTQSNAGRENESWGERRMSMKHKSLMPMNLQYFAE
                     GDDQKFSFDDFKSFVESNEEAQKFIQSQSQSVADKQLEAWKQNNLDKIKQDTIKEYEE
                     SKKNKSPEQIQLEKLQAEFEAEKALRVTSDNKAFVAEQIAGLELDGELKESISQFMLN
                     NLVSSDTDFTKNAVEGFTSVLNAINEKHADALKELQMKSAFGGTQQSNNQVQQNNETL
                     TNPEEQLGQILQQFN"
     gene            27852..28742
                     /locus_tag="IYI34_001051"
     CDS             27852..28742
                     /locus_tag="IYI34_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HAR0558930.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            28765..28980
                     /locus_tag="IYI34_001052"
     CDS             28765..28980
                     /locus_tag="IYI34_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558931.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            28992..29333
                     /locus_tag="IYI34_001053"
     CDS             28992..29333
                     /locus_tag="IYI34_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558932.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            29333..29704
                     /locus_tag="IYI34_001054"
     CDS             29333..29704
                     /locus_tag="IYI34_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558933.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            29697..30092
                     /locus_tag="IYI34_001055"
     CDS             29697..30092
                     /locus_tag="IYI34_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAR0558934.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            30094..30471
                     /locus_tag="IYI34_001056"
     CDS             30094..30471
                     /locus_tag="IYI34_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558935.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            30472..31080
                     /locus_tag="IYI34_001057"
     CDS             30472..31080
                     /locus_tag="IYI34_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR0558936.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            31080..31421
                     /locus_tag="IYI34_001058"
     CDS             31080..31421
                     /locus_tag="IYI34_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558937.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            31663..34452
                     /locus_tag="IYI34_001059"
     CDS             31663..34452
                     /locus_tag="IYI34_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HAR0558938.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            34442..35182
                     /locus_tag="IYI34_001060"
     CDS             34442..35182
                     /locus_tag="IYI34_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR0558939.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            35179..>37041
                     /locus_tag="IYI34_001061"
     CDS             35179..>37041
                     /locus_tag="IYI34_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558940.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNG"
BASE COUNT        12686 a         5547 c         7502 g        11306 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtatctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcca
     9421 tatcgacggt tggcgaattt aaaaatcatt cctgtaaaag tttttaaatg tattacataa
     9481 ttatatgata aatttttttc aaaatagtag taatctagca tatcttgtat aagattacta
     9541 ttaacgtttt ttacaagtac atcatctttt atatattttg aaattaatga tagataaacc
     9601 tgagttgttc gataggaact ttcttttaca gttggcttgt atttttcatc ccatttagtt
     9661 tttaattctc caaaagttag tggtaattct tcatttcgtt ttattttttc tgcgattttt
     9721 tcattcaaaa gtaagttagc ttgattccaa gcttggcgtg aattgctaga taaagtaacg
     9781 gacacttttc tctttttttc agttaaggga tccgtataac gttcggtgta cttaaatttt
     9841 ttgtcattta ttttttctaa ccacattatt tctattcctt tcgaatatac gttcctatta
     9901 attgttaaaa gaaaaagccc gaaggctttt tatatattta atcttttgac aattctaaag
     9961 atcgtctata ttgttcagct ttagctaatt ttgtaaatgt tactgttcca ttataatttt
    10021 ctaaaacaac tttctctatt tcgtctagtg aaactttgaa aaattctttt ctttgattaa
    10081 ttttattaac ttcttgtttt ctaaaatgat tatgcaattt agtttccaaa gacggagcgt
    10141 cctctgaaaa aatcatcgca tgaacgtcaa attcaaatgg aaccgatgca ctactcaatt
    10201 cttttattct atccataggt tctaatcttc tagtcatacc aattttatat atgtcttcac
    10261 caaaagaccc aatattacta attacataca cataaccggc tctagtattt aattctcttt
    10321 gtaatacatt ttcacgtata acttctaatt cttttaattt ttcttccagt tcttgaattt
    10381 tatttgcata taattctttt tccacatctg cattagcttt ttgcatatat gcgattaatt
    10441 tggaaatttc agaattgaat tgacgttgtt ctttatcaat acgtttcttt tcacgatcaa
    10501 tttctctacg aactttttcc tcttctatca tctgttcttt tattgctttt tgttgttctt
    10561 tttcttcggt aattttcact aaataagagt aatgaagttc tagttcttga agtttaaact
    10621 ctaaaagacc cttccttaat tcagcattgt cattcttgaa taaattatta attttatcaa
    10681 atatttttat gattttttcc ctagtcgagt ctatattaga aatagttagt ttattgagta
    10741 atcctttcac ttctgaatca aatggaatga gcattttttt ctttaaatta tttttctctc
    10801 gtttatttaa aataagaggg acagaaacag catcgtcaat aatatattcc ttttctttta
    10861 attttaacaa agaaagctta ttttttattt cagaagatga agtttcttct atatttgaaa
    10921 cgataagtga agaatagaca ttttttaatg tttcatcata cttttgattt atttcatcat
    10981 ttaaatcttc tttctctttt tttaataaaa taatatcatt ttttaacata gtaatttgtt
    11041 tatttaaatc tgatagtgtt tcttccttac ttaatataaa tctttctcta tcatgtaatt
    11101 tatttagttt ttcatcattt ttttgttcta attcataagc ttcttttttt attgtgttag
    11161 ctttcgacag tttagtgatt cctataatta ataaaataag tccaatcaat aataacattc
    11221 aaaactcctc atttctttga tataattatt gtagagttta ctcagaaatg aggaaagagt
    11281 ccatgtgaaa gcatgggctt ttttattttt aatgttgtaa tactaaatgt tcaagattat
    11341 gaggaagccc taaatattca ataatttgga actgactcat atcagataaa tcttgttcat
    11401 ctattgttct agttaacatt tcaattgcaa acatattagc ttcagcttct ataccataaa
    11461 tcatacaact aaggttgttt gctctaaaag ctgctgtgca aataccttta tggagtctaa
    11521 cgtgggctat ttcatgccat aatacaaatc ttttcataga aggtgatagg ttttgattaa
    11581 ttaatatcac ataacatctt ttattgtgta tcgtttgtcc taatgtttta ttccctaaat
    11641 cagtttcaat taaatctata tcagaatatt tgattatttt atatggatta atcgcatcag
    11701 atccaaactc tacaaataat ttttctattt cttctaatat ccagtatttg tttaaatgca
    11761 taccaaagcc cacctattga cgatattttt taggagtgaa ttttttcttt gctttaactt
    11821 tggttaaggc aagtgcaagt tgaatggcat cgtataattt ttccttatct tcatcgtcta
    11881 aaggttcacc ataataattt gtttcagcat ctgaattcaa gccttcaagt aatttttcag
    11941 cttgtaaagc aatatcttgt ttatcttttt ctgttaaatc ataataatgg cgtttatcag
    12001 tacgtcctaa aagataatca gttgatacat taaagtaatc tgcaactttt tgtattttat
    12061 ctccagatgg agtactttta ttccatctag acatgcttcc atttccgaaa tttaactttc
    12121 tttcaagttc tgccaacgtc attgaacgtt cttttaataa gatttttata cggtctaata
    12181 gcataggata tgcattcctt tcaaaatacc gacaaaaaaa gtatttttct gcaaattatc
    12241 tatttacaaa tagagaattt gcagttatac tagttttgta agcaagatat ttacggcata
    12301 caaagactaa aagcaataaa ctaatagtct ccccagacat cagtaagctc gctttttgtg
    12361 cgctgtattt tgtatgctta cataatagca tatttgcagt ttttgtcaaa cttattagac
    12421 aaaaatatta gaaaatattc gataaggggg tatgaagcat gactttaaaa cagcaagtat
    12481 taattgcttt agtaaagaaa ggttggagtc aaagagagtt agctcgacga atgggtatat
    12541 caattactta tttacgagat atcttcatcg aaaaaagaaa acctaaggaa cgtttaaaac
    12601 aaatcgaaga acttttggat atcaagttag aagtagattc taatgatcag ccatcagaac
    12661 aggaggcgta aaaatggtac aacgtttgag caaagcggag tttaaagaga aatataagta
    12721 ttctgaaagt acttatcaaa gaagaatgaa ggaatttaaa aaatcacgtt ttagtgaggg
    12781 gtataacgca gttacttcca aagaaataac aattgatgtc gatttatatg aacagtttag
    12841 aacatggcgt tctaaaaatc gatttagagt tagagctgtt tgttaatagc taaaaaatct
    12901 aaattcacca cttctgaaaa gaggagttgc tgctctcctt tcaagtttgc caaagtccac
    12961 gttttttcat tttggcaact cctcttcttg gaggtggtga cggcgagaga cgaaggaggg
    13021 atgacatgaa ggaagaatca aaaataggga ttttgaatga gaacaattac acaagattaa
    13081 ttattgaaac agaagaaggg aaaaaagtag cagaagttac attgaccgat gcaactcctg
    13141 ctgacggtta tcgtattcga ttaactccta aatatgatta accttttggt gggtgtggat
    13201 cgtttccgtg actatcacgg ctttgaattt ttccatctct gccatgaata aatagctcag
    13261 atccttgatt tttagaaatc tctctagcga tttttactgc ttctgatttt gtattagtat
    13321 gtgcagtcgc tttagaattt ccagcacctt tgacattcca accaccattg gaattaggta
    13381 caacatgttg attttttccc atagttttca cctccatttc tatagaaata ttttatcaaa
    13441 tagaaatgga gaaattcaat aaacgaaagg agaagtgata tggcatatac cttgcaacaa
    13501 gaacatcaaa ttctcggttt gattaaacaa cgcaggaaac aattacaaga tgaccgtgca
    13561 gcgcttagaa aagccgatga gctatcagat aggcaagctg aactaattgc ttctgaactt
    13621 gaggatttga gaatgctaga aataaaaaat agggagatta gattatgaag aagacagaca
    13681 cactttttat aggattcatt ttggggttat tagtgattgt agcgcaccaa agtattatcg
    13741 ggggaagctt gttcgcagca ttgatggttt taatcaatct gcttgattca aaagaaagga
    13801 gcaactatgg cacgagaaga agcgctaaaa atcggtaaag tgattgctga taatcggtgg
    13861 ggaaatagcc gccctattat tttaagcaag caacatatcg aaaagcaaaa agcatggcaa
    13921 caaataaaaa agtgactctg ccagccagca aagagtcaca tacaaattta taaaactagg
    13981 agaattatat cacatgaata agaaaattga aaatcttatc gaagaattga aacgtgaatg
    14041 ccaaaagcaa ggtgtttcta ttatttgtac agcccaaaaa gaaggggaac ttaaaagtct
    14101 cgtttatggt gagacaacag aaattttact ttgtcttgct atgcaagaag aacatttaga
    14161 tgaaaatctc ccattgtcag cacatattat gcgtagaatt gctgtggatg cttacgaaca
    14221 agcgaaaaat gaagaagaaa atcaaccttc aaatcatact tttgttatta ataacaagga
    14281 agatttagct gatgtgatga ctcgtattct caagggggaa tttcaatgat gaatgaaaaa
    14341 acaattgatc tactcattat tttatttttc acgttagtga ttgtagcgaa aagttatgta
    14401 atctttggct tgttattcgc aattaccatg cttgtgcttt gcgtgttagc aagcagaaaa
    14461 gaggttaaac atgaacgagt tagcaaatct tgacaactat ttaactgatc ctgattatgc
    14521 taaaccacca tatgaagcac caattgatga ggaggatgaa gatgagtaaa tctaccttag
    14581 aaatgagcca tcaagaatgg cttgaagacc gtaagaaagg catcggaggt tcggatgttg
    14641 gaacaatttt aggattgaat aaatggaaat ctccttatca actatggcta gaaaaaacag
    14701 gacaagtagt acttgaagaa tcaggaagtg agcccgctta ttggggtaat gttttagaag
    14761 aagtggttgc taaagagttt caggaacgta caggcaaaaa agtacgcaga agaaaccaag
    14821 tatttgaaca tccattacat ccatttttaa gagcaaatat tgatcgggaa gtagtgggag
    14881 aaaatgccat tctggaatgc aaaacagcca atcaatttct cggtaaagaa tgggaaggcg
    14941 aagaggtacc gcttagctat ctctgtcaag ttcaacatta catgaacgtt ctaaacaaag
    15001 actattgtta catcgctgtc ttaatcggtg gtcaaaaatt tatctggaag cggattgaac
    15061 gagatcaaga gctgatcgat acaatcactg aacaattagt agagttttgg gaaacaaacg
    15121 ttcttggagg tatcgagcca attattgatg gtagtcaagc gacagctgat ttcttgaaag
    15181 agaagtatgc agatgttgaa gacgttcaaa cgacgttacc aattcatttc gatgaactag
    15241 ttgaacagaa aaacgaactc aaacggacta agaaagaaat tgaatcagct atccgtcaag
    15301 tagacaacga gattatcagc gagctaggta aacgtgaagc cagtatcggt atcactcaaa
    15361 aaaacatcat cagttggaaa tttgtccgta cgagacgtat gaactcgaag aaactagcag
    15421 agaaatatcc agatgtcgca aatgatgaag agatttataa cgttactgaa tcaagaaggc
    15481 taaccgaaag ggagatcaaa taatatggca acaaatgaat cgttaaaaaa tcaattggca
    15541 gcaaagccac agaaacaagt tgcaccaggt caattaggac ttaaagcttt gatgaataca
    15601 ccaacaatga gaaagaagtt cgaagaggtg cttcatgaca atgctaatgc ttttatgtcg
    15661 aatgttatga ctcttgtatc taatgacagt tatcttgcag atagtgaacc gatgtctatc
    15721 atgagtggtg cgttaactgc tgcaacatta aatcttgggc tagataagaa tttaggttat
    15781 gcatatttag ttccattcaa tagtaaaaac aagcaaacag gaaaatggga aaagaaagct
    15841 caattcatgc ttggctataa aggatatatc caattagccc aacgatcagg taaatacaaa
    15901 gcattaaatg tgatcgaagt ttacgaagga gaactaaaaa gctggaaccg actgacagaa
    15961 gagtttgagt ttgatccaaa tggtagaacg tctgatgaag tcattggata tgttggctat
    16021 ttcgagttac tgaatggatt caaaaaaact gtctattgga ccaaacaaga aatcgaagct
    16081 catcggattg ctaacaataa agatcgcgat aagacaaagt taagtggtgt gtgggcatct
    16141 gattacaatg caatggcacg aaaaactgtt ttgagaaatc ttctttctaa atggggaatc
    16201 ttgtccatcg aaatgcaaga agccaccaca tcggatgaga gagtccaaag ggttcaagaa
    16261 gacggcagca ttattgctga aacagaagtt gaagaagata ttcctgaaag aaaagaagca
    16321 gaggttatat ctgaagaaaa tgaagatgta caaactggat tatttgatgc atctaatccg
    16381 ccgttaaaca aataatgagg gagctttctc ccttgctttt ctagaggaga aatacgaatg
    16441 aacacaggat atgtcaaatt acatcgaaaa gtgatgaatt cattcgtttg gaccaatcct
    16501 tatatgtata aattatggaa cttgtgtttg atgaaagcga gtcatgagaa tcgcaagata
    16561 ctttttaatg gaaaagagat acaagtgaac agcggagaat tcgtcacagg gcgcgatgct
    16621 atcacatctg agatgaacga aggcgtgaaa cgtgaacatc aagtgaacag cggttctgta
    16681 tggagatggc taaaacaatt tgaaaaaaac ggaatgttga acatcaaatc aactacgaaa
    16741 tacagcgtta tatcaataaa aaactggtct ttgtaccaaa gcagtgaaca acaaatgaac
    16801 atcaaacgca caacaagtga acaacaagtg cacacaaaca agaatgaaaa gaattataag
    16861 aatgaaaaga ataataaaac atcatcgtcg caaccacgca aaaagcgtgt ttacgacacc
    16921 gactcagttt actacattct cgcggaggag ttattcaaac agatttgtca gaatcaggaa
    16981 atcaaaaagc cgaatctgca aagttgggca gatgacattc ggaaaatgat cgagatcgac
    17041 aagcggaccg aaaaccaggt acggggaatg attgaatgga gtcagcacaa tgtgttttgg
    17101 gcatcgaata ttttatctgc taaaaaattg cgagaaaaat acgacacaat ggcagcgcaa
    17161 gcaaatcgtg attataaaac aaaacaaact aaaacgcttg aatacgagaa atttagcaca
    17221 gatgagttgc ctatttgaga ggaggcgtag gcatggaaac tgttggggaa atcatggaaa
    17281 agctgataca gaaagtgctt gtccaacgtg gcgaatgtcc tgaatgcgga cagcctttgt
    17341 atggatggcg tacgaagaac cctgatggct cagaacgttg taagccaacc tgtatgcaat
    17401 gtgggtataa agcattgcga gttcaggaag acttacaaac agaacgaatt tacaacgaga
    17461 gcctgaaagc aagagcaatc aattttttca aaggtggttc tgttgtgcct aatcaagcgt
    17521 tgtttgattg cacattgcag aattatcaaa ttgtcgatca agaaacaaga caagcggttg
    17581 aagtaaccaa acgctttgtt aattcagtct tgttaggaaa tccaagtcac cttgttttaa
    17641 ctggaaaaca aggaacaggt aaaagtcacc tagcgatggc agcggcttgg gaagtcttga
    17701 agcgatcaaa ctacgataaa aaaatcttat ttatcgggtt acaggaaatg ctggatcaaa
    17761 tcaaattttc gtacaacaat cctgaactca gaaaaacgat tgagggatcg ttgattgcag
    17821 atatcaaaac agcggattta gtcatcattg atgatatcgg ttcagaacta ggaaaagatg
    17881 catcagatag tcgagcgttt ggcataaaca cgctaaattc gttcttggat gcacgacaga
    17941 acctagcaac gattatcaca acgaacttgc ttggtgaaga actgaaaaaa gcttacggta
    18001 cgagaacaat atcaagaatg tttgtcaact ctgatggatt tacgatggta ttttctcaaa
    18061 cagcagacaa gcgcataaaa ccagtcaaag gagcgatcgc atgaatttga ctaaacaaga
    18121 aaaaagaatc tgtcgtcaga taatgttgca aattataggt gatcgtgaca tggacgaatt
    18181 atctcaacaa gagagtcgaa tggttcggaa gataagtaag ctaattattt atcgggatac
    18241 agaaagattt ctcactgttt taggtaacgt ctacgagaat ccagagttat tggaggtaga
    18301 gtaattgcca aaaatattag atgcatgttg cggtagtaga ttgttctggt ttgataagaa
    18361 caattctgac gtaacattta tggattgtcg tcagcagtat gaagaattat caacaggtca
    18421 tgtgatcaat gttgatccag atgtggtcgc agactttcgc gatatgcctt ttgaagatga
    18481 tgtgttcgat atggttgtat ttgacccgcc acacttaaga catgtcggcg aaaactcatg
    18541 gcttgccaaa aaatatggaa aattagacga actttggcca gaagacatca ggcaaggttt
    18601 tgcagagtgt atgcgagttt tacgaccttc aggaacatta atttttaagt ggaacgagga
    18661 acagatacca ctttctgatg tgttagaggc tattggagaa cagccattgt ttggtaataa
    18721 acgaagcaaa acacattggc ttgtgtttat gaaaaaattc agctaacgga ggaaacaaag
    18781 gaggaaaagt aatgaagatt gtattaaata aatgttttgg tggctttggg ttgagccatg
    18841 aagcaaaaat ggaaattttt aaaagaaaaa atattgaagt attcccatac atcaataact
    18901 ttagctatga ctcagatgat gaatacacga gatacacagg ccaaaagcta ggttcgatgg
    18961 attttatcta ctatttcaaa aaagacccga aaatcgacaa agttacagga atttatagcg
    19021 aaattgaccg actgtacgga attgctgatg attccagttt ttcaagtgat tcaaacagag
    19081 gtgataagga cctcgttgct gttgtcgaaa aactaggaag tgaggccagt ggaccatatg
    19141 caagtcttaa agtcgttgat atacccgatg gggctgagtg ggaaataagt gattatgacg
    19201 gcgttgaaac tgcacattat ggttttcaaa caggtagtat ttagtccact aatgacaact
    19261 aaagaggagg cagaaggatg agtgaagatg aatactttga acagtagtct attaacgaca
    19321 gagattttta actggaaagg ggtgtagtca tggacgcatc ggaagtagta aataaaatta
    19381 atagtctcaa agaggttttc ggagacgtcg aagtagtgat aaacgcacaa ggctatgaca
    19441 acgaattttt taagcagatt accgacatta gcattcagca aggaatggaa gacgaagacg
    19501 gaaactttat cgatgaagta gccattttag tgacttgcga gtaagtaatt gacagctaaa
    19561 gaggaggaag cggaatgaaa ctaaaagacg gattttactc cagcggccac ggcatcggcg
    19621 gtttaatgct agatatgccg acaaagaatc ctaaaacacg taagaaacca aaattcaaag
    19681 tcggtgacat ggttcgctgc gaagcagaag gattcatcta tccatttcgt ggatatgtag
    19741 aacacgtcta taatcactca gcaatcattc gcattgaaaa cacgatggaa tgtgataagt
    19801 ggttagcgaa aagcaaagag aatttagcag tggtgagatt ggtggacatg gaagttataa
    19861 aatcttaatt ttgctttttt acaactatcc tacaacaatg agcgttattc cacaactgga
    19921 aggagaagag gcaattgtga acattttaga gaatatagat attaagcaaa caaggaaaaa
    19981 tgctagacga ttactaaaaa gatacagaaa tttagaacgt ttggtcggac cagtgaaaat
    20041 agacttttcc gtgatgactg ttaccaaaaa tttgaaattc acaattgaca gtcaaaacga
    20101 agaaatcatt gaagcgataa gtactcgaga ttcggttatc gaagcattaa cacggctaag
    20161 tagaatccat ttccaggtac tttattatag ttattgtttt cctaataaga tgtcaatgta
    20221 tcaaatagga gaaaaattag gatattccga tagaactatc gagagaatga aggcagtagc
    20281 tttggttgaa tttgctgagg cgtataaatc aggagaactc atttcacgta caaaataaaa
    20341 aaagtcggat tcctccgact atgaataata tttccgacat aagtattata tcataattgg
    20401 gggaatcaga ggatggtact tttcgatgta aagaaatatg agacaccaag cgcgaaagat
    20461 gttgatatgg aacgcacaaa acataatgtc gctgtgtttc tttcagcata tctatcagct
    20521 agatgtagag tagggcaaca tcgagaacca aaagtgacag catcttattc cttggttcca
    20581 ccttctacag ctgatcatat atttgaagca gaaagaatgc tgatcgataa agaagaagca
    20641 caggaagaat ttgagtatct gcataaattg tttgttcgag gctattcagc gatacagcat
    20701 ccgcataagc ctgatgtgac tgaaaggcgc aagaagatat tctatgatcg ttatatcaat
    20761 ggtctgccca tttatgtaac tgctcaaagg aataatacta gcgaagaatc ggttaaagta
    20821 gaatcaaaca gaattatcat ccaatttgct tcatcgttag aactggttgc tttcaagtag
    20881 ccagttttta cactttttat acccttttat taccaatttg gtttccattt tatacctttt
    20941 ttataccaat cacttaccta ttcaacgttg tattatgata gtgtcgaaaa attaggaaac
    21001 aggatcgaca aaataaaatg taagggagga aatctccctc atcgtttaat taagcttcga
    21061 taggcagcaa cggaaatatt aagaataagg atgtgaattt caactccttc taaattgttc
    21121 ttattatcta tcatccgttg ctgtctattg tttttttaat tattcacacg ataactaaag
    21181 gtaagtgaag agaaatgatt ccattaataa tttcaatttt tgcgctttgt cttaatgtat
    21241 atatgattgg atttaaaaat gggcaaaata aaaaatagta gcagccaaga ataattttat
    21301 agtgtcactg tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa tgcttcggca
    21361 accatgcaag gttcgattcc ttgccagtga ctttggcaac cgaggcatcg gcggtttaaa
    21421 aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg tgtaggttgc tattacataa
    21481 ctggttaggt tagattgaga tttgggattc ggtacaaatg aatcgtcaaa tgtctcaagc
    21541 acaggatcgg aaacgtccct gcctgtgcat tacatattag atcactcttt gagtggtctt
    21601 tttctttaca taaaggaggc tacataatga gaaactactg gtatgtatcg ctaactaatg
    21661 aatatcctcg aaccattgat gattgttcag tgcgtgttgt gcgttctgta caaatcaaag
    21721 ggaagtactc tattgtcgaa atggggagag aagcagaacc atgtgagatt gatgcgtgca
    21781 agctcgttta ttgcggtcat ggtttctatg atgaaccaaa cattcaaaat aatattaaca
    21841 agaatttgag ggattagaat gcaagaaatg gcttaaaaat ctccaatcag gacaaatcaa
    21901 gttagacaaa gtttccgatt taaagatatt gattgaagct gatttaatgc taaaagatat
    21961 tgaaaattag aaaacaaaac tcaacctaaa cagaatgtga ggtggtgtgt attgaatggc
    22021 aagacaacgt gatccaagac gtgatgaagc caaaagaatt tggttagaat ccaacggaga
    22081 aaagcagtta aaggaaattg catctgaatt aaatgtttca gattctcagg ttagaaaatg
    22141 gaaatcgcaa gacaaatgga gcgctgaatt gattagtaac gttaccaatg gcaaaagtaa
    22201 cgttactaat caaggtggcg ctcctattgg taatcaaaat gctaaaggta acaaaggaaa
    22261 tagccgagct tctccgccag tgggtaataa aaacgctttg aaaacaggtg agtatgaaac
    22321 aatatttttt gacacgttaa ccgataatga gaaggacatc tattctagtt tgaatgatga
    22381 tccttctttt gctttgtccg aagaaatacg acttcttaaa ataagacaac ttcgaatgat
    22441 gaaaagaatc caacaagctg aagctggact aaatgatgaa gaagtcgaac gattgcagca
    22501 gctaagaaag attaaaactc cgattgaaaa aaatggtaaa aagctagaaa tcaagcgtga
    22561 ggttatgcaa gatgtgcaga ttagcagaaa aaaacatcgc aaaattgatg atattctttc
    22621 aattgaagat tcattgactc ggattagcaa ccagttagct aaagccatca agcaaatgaa
    22681 tgaactttat atgaatgaat acagaactga tttaattaaa gctcagactg ataagatcca
    22741 agctgagaca aatgaaattg gcggaaataa ttcaggtgaa gaaatagaag aatggaaaca
    22801 ggcagtttta aatgccgcaa acaaacgggc ggtgaaagaa aatgaataat gaatttattc
    22861 cttttgctga tattggttct gccattgatt attactatga taaaccagta gctttttgcc
    22921 aagatatttt gcatttgaat cctgatgaat ggcaagaaaa tgttttaaat gatttagctg
    22981 aattttcaaa ggtttctgtt cgttctggtc aaggagttgg aaaaacagca ttagaagcag
    23041 gagcaatact ttggttctta acgtgtcgac cctacgctaa agtaatagca acagctccga
    23101 caatgaagca actttacgat gtactttggg cagaggtagc taaatggtta aatgatagct
    23161 tgatcaaaaa cttactgaag tggacaaaga ccaaaattta tatggttggt gattcagagc
    23221 gttggtttgc tacggctaga acagcgacta aaccagaaag tatgcaaggt tttcacgagg
    23281 accatatgtt gattgtggta gatgaagctt ctggtgtgtc tgatccaatt atggaagcta
    23341 ttcttggtac gctatcaggt tttgataata agctgttgat gtgtggaaac cccaataata
    23401 ttgaaggtgt cttttatgat tcccataatt cagaccgtga taaatacaga gttcataaag
    23461 tatcaagcta tgatagtaaa cgtacaaaca aagacaatat agaaatgatt cttaaaaaat
    23521 atggaaaaga aagtgatgtt gctcgtgtcc gtatttttgg agaatttccg aaaggtgcgt
    23581 tggattcatt tatcagtctt gaaacagttg aattggctac agaaaaacaa attagtgatt
    23641 ctttagtcaa taaaacaaag gttgctcata ttggtgtcga cgtagctcga tatggtgatg
    23701 attctacgat tctctttcct agaattgcta ccagggcatt ggagtatgag aagtattcaa
    23761 aacgtagcac catggaaaca acaggatatg tcatcaacat ggccaagaat ctaatgagtc
    23821 aatatccgag tattgataaa gtgatgatta aagtcgatga cactggtgtc ggaggtggtg
    23881 taaccgaccg cctagaagaa cttatagaag acaaacatta tccttttgag gtgtttggag
    23941 tgaataacgg ttcaacatca gaagacgatt tttacgataa tttaggtact caactatggg
    24001 gaaacatcaa ggaaatgtta gaagaaaata tgacagcaaa tcttaacgga gaacagcctg
    24061 ttattgaatt gccttctgat agttcgttaa tcaaagaatt aagtactcgc aaattcaaaa
    24121 tgacaagtag aagtcgtata cgtttagaaa gtaaagatga tatgaaaaag cgaaatattg
    24181 gtagtcccga tattgctgac gcactggctt tagcgtttta tgagccacca agtcactatc
    24241 aatttattca attttaggag gtgagctttt gattagtaca gtattatctt tagagagata
    24301 taagaagcta cgtgttaaat atgcaacaca aattgaagat ggaatgtttg atccgaatgg
    24361 ttttatagaa gatatgaaac cattttttgc tgatcgcgaa agaaaatatc tagcttatac
    24421 tagcgaaaag aatgaaatag ataatagacc aaagcctaac actgatattg taaaagttaa
    24481 taataaactt catgctggta tgtattcaat tgtcgtagac caagcagtca atcattttac
    24541 tggtatacct atcaaatggg attacgatgt atcggaacaa aaaagaacac tcattcaaag
    24601 attaaaagat aaattcttaa aaaatgatat agaacttccg acagttccag aagcttttaa
    24661 caaattaaca agcaatcttg attccatgag attcgcaatg cttgattctg aaactgcaac
    24721 ttttcaagga gcttgtggag tagctttccg attgctagag cctgtggaag aagatgatgg
    24781 ttggaagtta agagcaagta atattgaacc ttggagagca gaaagatatg gaaatgccgg
    24841 aatctatatc aaagaaaagt atgactctta ccagaaaaaa ttttttcaag aaatgaaagt
    24901 tatgacaaga aataaaattc tgacatatgc ttgctatggt gatttgaatt ttgttacaag
    24961 cggaacgttc aaaaaaattg atgagaccga taaccctttg gggacgatta ccttggcaga
    25021 atttaaaaat aatacgaatc gttattgtga ttttgaagta gctgaggaaa ttggagatgc
    25081 aattgatcgt gctttgtctg atcaacaaaa cgaaatcgaa caatttaagc ttgcgtacat
    25141 gcttgttact ggcacaacaa tgagcaaagg cactgctaaa gaaatgatga atcagctagg
    25201 gattatcaac ttaaaagccc ctactgcaaa agctgaatat gtcacaaaaa atttagcaaa
    25261 agatttcaat gaatatcata tggatctatt gaaaaaacag ttttacacca tttgtaaagc
    25321 aatcgatttt aacgatgagg tgtttaaatc aaacagttct ggagaagctc gcaagtggca
    25381 aatcattagc ctagaagcga aaacaaatac taaagagcaa tattttagag aaggattgaa
    25441 agaatgcgca gaaacaattg ctgcttttct taagtttcat gacaaagtag aaattgagcc
    25501 tgaaaaaatt atttttactt tctctagatc tttgccaact gatttaagtt atttagctga
    25561 agctttacct aaacttgcac catacgtatc caaacgaact attcagagcc aaattccatt
    25621 tgtaacagat gttgattatg aaaatgaaat gatggaattg gaaagtggaa gtgcttatcc
    25681 agatagtgaa tacaattttg gcggaggtgg caatagtgac gatagaaacg aagtattgga
    25741 ccaaacgtcg agaactggag gatcaagcaa ggctcaaaca agaaaatcag acacttaaaa
    25801 aattaactag tgtatttcct gaagcactga aagagataca agcaaaacta ttatcacaag
    25861 ctgacttaca caatatcact tatccagaaa tgatggagtt ttacagtaca agtaatcaga
    25921 aaaaatatcg tgaatatgtg gaaaaaaatt ataagtcatt aaaaatgtat gatgcaaaat
    25981 acaaagagtt tatcgatgaa ttttttccac catttgacta tgcaaaagtc aatcgcttat
    26041 tacaaatacg atcagatgta tttaaaattc ttgcagaata tgcgatggat gcagatgtga
    26101 atcaatattt ttctgatcgc ttagaggaaa ttcttcaaag aacatattct tctaatgcta
    26161 atgtttttgt tcaactttta aacgttgata taccaaatta tttaccagaa aatgaaatta
    26221 aacattattt gaactatcca tggtcaggaa agacattttc aagaagactt tggggaaata
    26281 tttcatcgct tgaacagaaa ctctctaatg ctattgtaaa aagtgttgct agtggagaag
    26341 gcgttataca cgcattaaac accatgagat tagactcaga aatttgtgac atgtttaagt
    26401 tagaagaatc aaagtataac aaagcgatag aaaatctcgt tcgaacggag tatgcaaaat
    26461 ttgcacaaga tggtattgaa aaatcatatt tagaaacagg tattgaggaa tacaacgtat
    26521 tgactgcaaa agatgagaga gtttgccgga tttgtggagg aaaggcaagt aagaatccct
    26581 ataaactgaa agatgctgtc ataggtgaaa atcgagcacc tttccatagt cgttgtagat
    26641 gtacggatgt tcctaattta ccaaaattag gaaaggatat tgatgaagaa tatgaccgtt
    26701 tatttggcga tttattagat gagtttgcac atgattcttt tggaattaat ttgaaacgga
    26761 ggaagtagaa tgaaagattt ttttgaagca gtactaacaa ttaatgtaaa tgctgatatt
    26821 gcagaagcct acaaaacagc tattgagtct gagaaccatc ctaatggctt gagagaccat
    26881 tggaatggca attatgccta cgtggttatt ggcgatcaaa ctgttaatta tcaagataat
    26941 actccagttg ataagaatac cgttaattta acgattcaat tattatctca ttcattacca
    27001 aatttaaaag aaacagttga ttggtatgaa aagatgggat gtattgttgt tagaactgac
    27061 tacaaagaag gaaagtctag taattaggct tttttatttt gtccgaaatg acgttaaact
    27121 agcgcaatac tgggcttggt tgaatggtgg ggcgcaacta ttaaaactca aagcaatgcg
    27181 gggcgtgaaa acgaatcgtg gggcgaaagg agaatgagca tgaaacacaa atcattaatg
    27241 ccaatgaatt tgcaatactt tgccgaaggt gacgatcaaa agttttcttt tgatgacttc
    27301 aaatcttttg tggaatccaa tgaagaagct caaaaattta ttcagtcaca atctcaaagt
    27361 gttgccgaca aacaattgga agcttggaaa caaaataact tagataaaat taaacaggat
    27421 accatcaagg aatatgagga atctaagaaa aacaagtcac ctgagcaaat tcaactggaa
    27481 aaactacaag ctgaatttga agcagaaaaa gcgttgcgtg tgacaagtga taataaagca
    27541 tttgttgcag aacaaattgc tggattagaa ctagatggag agttaaaaga gtctatttct
    27601 caatttatgc taaataatct tgttagttcg gatacagatt tcactaagaa tgctgttgaa
    27661 ggttttacaa gtgttttgaa tgcaattaat gagaaacatg cagatgcatt aaaagaacta
    27721 caaatgaagt ctgcatttgg tggaactcaa caatcgaata accaggttca gcagaacaat
    27781 gaaacattaa caaatccaga agaacaatta ggacaaattc ttcaacaatt taactaggag
    27841 agtgaaaaat tatgaaaaaa acatctttaa ataatttaga gtatttggat atttcaccag
    27901 cgattaatgc tatgcaagta ccaaatacac cttttttaag ctatttactt ggtgctggaa
    27961 aaacagagcc agcaaactcg acagaaatta aatggcgtga atatgatatc aacaacgatg
    28021 attcttctga aaaacttgag ggcggagaat atccagatgc tgaatcaggt cgaacttggt
    28081 ttaacaacta tactgaaatt tttagaaaat caacctctgt atctggtaca ttagatgcta
    28141 ttaatgtgaa tggagtcgga aatgaattaa ctaatcaagt agcactacgt ggtatggaaa
    28201 tgaaaattga tttgaaccga aaattgatta ctggtgtaaa agctgatgaa aatggttcta
    28261 aaggtcgtcg aatgaatggg attttgaact tgatcaattc agcaaataag gcagaaacag
    28321 ccactgcggg tgcagtaaca agaaaagata tcgatacttt atttaaattg atgtatgaaa
    28381 aaggttatat gggagaaaaa ctatgcttga tttctccaga tatgcaggag ttaatgactg
    28441 atgagttaga tggaaaatca acaaaaattg ttcagttcgg ggaaagagta acttttggat
    28501 tgcaaattgg aaatatcgtg tctaattacg gtacaggtat tgctctacta gaaccatcat
    28561 tgccaaaagg aacaattgcc gcaatcgata ctaattatgt gaaactacgt ccattacgtg
    28621 aatggagagc agaagaactt gcaaaaacaa cggattctag acgtatcggt cttgtaggtg
    28681 aatactctct tgaatacaat gcttcaaact ctggggcaat tttaaattta aagtctgaat
    28741 aaaagggagt tagtactccc ttttttgata ggaggaatta cagtggtaaa aaaagatgaa
    28801 actaaaaaag atgaagtcgt gaaatataga gtaggtaaaa ctaaaaattt tgttgggttt
    28861 gttcatccta aaactcgtag atttatcaca gcagattcaa ataacgaatt tatcatttct
    28921 atagatgata aagaagcaat tgcaattttg gaagatgcaa ttgatgttaa tgaaatttag
    28981 gaagtgatct gatggatgaa tcgctaaaaa cggaaatcat tgagtctaca aaagaagatt
    29041 ttccagattt gagtgaagaa cgcataacta atttattaga aataattttg ctagaaattg
    29101 aatcatacaa cacttgtaaa aatgatattt catgggaaaa gttaaaaagc gtgattagtg
    29161 aagtgttgta taaaataata aaaaatgaat cagaaaaaac agtatcttca gttagacgtg
    29221 gcgatacaac gattagttat gcttcaacaa caaatgatgt tagtgagtta cttctaggct
    29281 atggtgattt gatacgaaga gttattggtt gtggaggatt ggagtttttt taatgaatga
    29341 agcagatatt ttagaaacta cttatgaaga tagttgtatt attgaaagac tgacggacat
    29401 tgaagattct aatacaaata ttactattca agattacaaa aaagtatatg ataatcctat
    29461 ttcttgcgct ctttcacaag gtcaaatcga tggactagca gtcatagaag atggagagat
    29521 ggtaaatgtt tcaactgaca catataaatt atttgttcat cctaagatta aactaaaaaa
    29581 aggagatcga ataacaataa ctcaaaaggc cagtggctta attttttctc tatttgctac
    29641 taagcctttt tactatccta gtcattgtga agtgaatttg ataggaagtg agaaaaatgg
    29701 gtaatctcaa atatgaatca aatgcagaaa agatcattga gaattttaaa aatatgactg
    29761 taattgctca aaaagaagga atatccttcg ttaacgattc aatgaataaa gtcgttagtt
    29821 tagctaaacc attaactcct gtaaaatcag gtaatttaag gcgaggatat cgtgtagtaa
    29881 aagctagaaa actatcaagc ggtcgaattg ttggagaagt tataaacaat gaacattatt
    29941 ttaaatatgt agaagaaggt cataggacta aaaatggtgg ctttgtcaaa gggaaattca
    30001 tgttaactcg tgcaacgaat cttgcaaata tgtcttatat tcctcgaaga tttaaacaaa
    30061 tggcaataaa aatcgttaag aaaggaaagt aacatgaaag atgaaatcat tgctgcaatc
    30121 agtagcaaat taaaagaaat ctatcctgat gggacaatat atcttgattc agttatgcag
    30181 tcaactaaag atttttattt cgttttatca gtaatggaat ctggaactga aaatgtagga
    30241 attgatattc aaaatgtttc tttcttaatt gatattgcat tgattgataa taaccctaat
    30301 agaaatttaa ttaatgaatt agtctcacgt tgtgggactt tttttaatac gattacaatc
    30361 gatgagcaaa tattatttcc aaaagcctat ttacctgatg aagcagatgg tgttcaacat
    30421 attcgtttta cattagaatt tccacaatat attgaatgga gtgaaagata aatgggagaa
    30481 aaaagaagta aaaccggaat tatttctgtc gaaaaaccta cttggtttcc tttggaagat
    30541 gagacaggga attttccggt atatggtact gcgttgccaa tgggaacagc agtaagtatc
    30601 aaaccaacag ctaattatga aacaacacaa gattatgggg attcagtggt tcaagatcaa
    30661 tttacagctt ttggtggtgc agaagttgaa ctggaagcaa atggatatac tcataaagtt
    30721 ttatctacaa ttactggagg gaaaattgtt aaaggtggtg ccttgcgttc tggagaagat
    30781 attgcgcaag atggtgcgtt tgcatataga cgtaaaaaat cgaatgggaa ataccgttat
    30841 acggtctttt ataaaggaca gtttgctcta gattcagatg aaacatcaac gattgaagga
    30901 agtaaagtaa gttttactca tccaacttgg aagggttcat ttgtagatgt accaggactt
    30961 ggatatatgt attcagttga tgaagatgat gaaggcgtgg ataaagcaat gatcggaaat
    31021 tggtttacaa aagtagcaat tccaattgaa gaagcagaac tttcaggagg tacagaataa
    31081 tgtcaaaata tcaaacaacg attaaattaa cgaaaaaaga cgaagaaggt aaatatgagc
    31141 aagtacaatt taaatctgct gaatttttac caggaacagt tgtagaagat gctgcaggag
    31201 ttatggaaga aatgcaaaca gcgactgata agcaatcagt taaaaaagct ttaagtcgtg
    31261 cttattcatt tattgcagat actttatttg agggacaatt tactggtgaa gattattgca
    31321 aaggaattga tgctcgtgag attgcctcat tgacaggaaa attattgaag tctgttactg
    31381 caggttttga tgaaacttat acagagacga aaaaaaagta agtgaggcgc tcaagtcacc
    31441 ttcatttaag tattcgatta cttaccgaga attagatata aagacgcaat tactcgaagc
    31501 aggttggacg ttaccagaaa ttgaacgtac tgacttggat gagttaatgc gtctttttgc
    31561 ttttagagat gcggtaaaag agcatgaaga tgttgagtac tatgataatt tcactcaatt
    31621 ttaggaggtg atactttgaa taacgatgac ctaattctga agatgatact agatgaatct
    31681 ggttttactg ccggtatgaa taatgctgtc aaaaagttga actcttttga tgaaacaatc
    31741 gaaagaaaaa gtagaaatag tggcagttca ttaggtagta tttggaaaat atttgctgga
    31801 agttttctag ctagcggagt aactagaatc gtaggagctg gctttgatct aatcaagggt
    31861 tccataagtg gagcggttga tcgagtagat acgatgaata acgctctacg aaatttccaa
    31921 aatatgggat ttagtaattc agaaattatg aagaatatcg gaaagaatgg gcttttatct
    31981 caaggtattc aaggacttcc tactgctttg aatgatgcga taagtcatgt tcaacttctt
    32041 gcttcttcta caggtgattt aactcgttca actcaaatat ttaaagcttt aaatgatgga
    32101 attcttggtt tcggtggttc aactgatcaa gttaatgaag ctgttattca attatctcaa
    32161 agtttctcaa acggaaaagt agacgcacaa acttggaact caatgatcaa cgctcaactt
    32221 ggacctactc tttctgctat tgctaaaaag atgggaatta caatgggaga tctgaaagaa
    32281 ggtttatctc aaggtaagat ttctgttgaa gaattccaaa atcaattaat agaaatggat
    32341 accaaaggtg gcggaggact taaatcatta agtcaaatcg ctaaagattc tactaaggga
    32401 attaaaacct ctatacaaaa tgcaaaaaca gctgttacac gtggtgttgg tgaggtaata
    32461 gaaggattaa ataaagcatt agtagattca gatttaggtg gatttaaggg aattattgat
    32521 aaagtgggca gttcaatgga atcgttctta aaagtaattg ctgcaaatat tcctacagca
    32581 gtatcatttt taagtaattt atttaacgaa attcaaaaat ttggttctgc tttgaaattc
    32641 atgatgccct ttcttgttcc tgcagctacc gcttttggag cgttcatgtt tcaacttaaa
    32701 ggtataccag caataataaa aagcttcaat aattttaaga atgccataat tggcgttgga
    32761 aattcattaa agattatggg ggcaatagct gctgctaatc catttgtttt aattattggt
    32821 gcagtcgttg gtgcaatagc tgttttcggt tactttatgg caaccaatga agagcttaga
    32881 aacaaggtta tatctatttg gaatgatgtg aaagattccg taattggtgt attaaaaaat
    32941 ataaaggact ggggaattga tacttggaat tctgctaaag aaatggcatc aaatgcagtt
    33001 gagggtgtca aagatgcttg gtcaggaaca aaggaatggt tttctaacac ctggaaaggt
    33061 attaaggatg gggccacagg tttatttgat aaaacagtag agacttcaag aaatgcagtc
    33121 gatagtgtaa aaaatgcatg gtctaatatg aagaaatggt tttctgatac ttggcaaagt
    33181 ataaaagatt cagccagaga aaaatggaat ggaatcaaag gttctattat gtcagttgct
    33241 ggtccattga ttacaggtat aaagaatgca tttttacatg ttacttttta tctagagacc
    33301 ttatggaata atttagttga gattggtaaa aacgtctttg agattttaaa aaatgttata
    33361 cttgcgccgg ttttgtttat tacctccatg atttctggtg gatgggaaga aacaaaaaat
    33421 aatatgattg ctgtttggaa taatattaaa gaaagtgcca taaatatttg ggaatctata
    33481 aagaatatat ttgtaagtta ttttacaaat atttattttg ctgcactcaa tatttggaca
    33541 gggttcaagc ttacattgat aaatatttgg aatgaagtgg taaatcaagc taaatcgatt
    33601 tggttaaaca taaagtattt ttttattaat ctttggattg atataaaata ttttgcaatt
    33661 cagaaatgga ttgaattgaa gttttctatt attcaaactt ggatcgattt aaaatataat
    33721 gccattacta cttggaataa tattaaacag tttttcaaag atacttggaa aaatattaaa
    33781 gatacggcat acaatacgtg gatttctata aaaaatacca tgattaatac atggaataac
    33841 attaaggact ctttctggaa tattgttact ggaattgtta attctgctga aaatgcatgg
    33901 acaaatctaa aaaatggtgt ttcaaatgct attaatcggg taaaagaaat ctttaattcg
    33961 ttaagggaag tcaatttatt tgaaattggc aagaacatca ttgatggact tataaatggt
    34021 gtaaaagaaa agtggaatgc tttgaaagat actatcaaag gtattgcagg taatatcaaa
    34081 aattggatta aaggagcttt agatattcat tctccatcaa gatggatgcg tgatatggtt
    34141 ggtaaaaata ttgttcaagg tattatcgtt ggtattgata aagagcaaag taagttagat
    34201 caaaccatga caaatttagt aaaaacaccg tctgttcaac ctgtagtaaa tggaataagt
    34261 cacctaccaa ttacacaaac aaagcaaaat atatctgcta gtgaaacaaa agaaattcat
    34321 ttacatttaa atgtttatgg agatttacct gattcaatgg ttcggaagat taccacaaaa
    34381 attaaaacag aattgacaag acaaatgaaa cgagatgctg gtgcagtagg aggaacatta
    34441 tatgcaactt aagagaggac aattttttat taatcaacat tattcttctg aatttaatgt
    34501 gtatattcaa aacaggcctg cttctgtttc agctagtcga gtaattgaat tgagagagcg
    34561 agaaggaaat gattctatca ttattgataa aggctattac aaaaatgtga ctagaaagat
    34621 tgagtgttac tataaagcac cttcaattga tatggtgcag gagtgggaag atcgaatcac
    34681 tgaatggtta gatatgtcgt cttatagtga ttttattctt tactatgatg aacaatatat
    34741 ttatcaagct gtagtaatag aaggacctga atttaaagga acaagaaaaa caggaaatat
    34801 tgtccctttt gaatttacag tgagtattcg accatttaag gaaaattaca atggtagatt
    34861 tactattcga caaacagaaa cttttgaaat ttataaccca gagaagtatt cttcaaaacc
    34921 gcttattaaa ttgagtggtt ctggagatgc ttctttttat attaataagg ataaatatga
    34981 tttgaaatcg ttggacagag aattatttat agattctaaa ttagaagagg cttatcgaaa
    35041 attagatggt aatttagaac atcaggatca ggtcacctta tttttagatt ttccattttt
    35101 atatcctgga aaaaatgaaa ttaaatggac gaaaaatatt cattcatttg aaataatgcc
    35161 taggtggtgg agaaaagtat gaaaccaaga atatatagtc ctactgaaac agatttctca
    35221 acgaatggct taggaatttt aaaggattgt acaagatgtg gaatatatga agtagctaat
    35281 ggaaaatatg aattagagtt ggattatcct ttaggaacta gatttgatga atattttgaa
    35341 aatgactatc aaataaaagc aaagccaaac gatcaagaag agtatcatat cttttttatt
    35401 gatgataaag atatagatac ttttttaaat acagtaacta tttatgctca gagtcgtaca
    35461 aatcgacttg gaagacgggt agtcactctt gtggaagtgg actctaaaac tggtcaagaa
    35521 gcgatatcaa ttattgaaac taaaatggat aaaaaatctg acatacgact ttattctgat
    35581 attacggctg tttctagcac aatctttgaa gcgagaaatg ttttaaattg tattgctggt
    35641 gaacaaggat cattgcttca atattggggc ggagaaatta aacgtgaacc atttaagctt
    35701 tctttgttaa agcgaagagg tcgcaataat gttggaacga ttcggtatgg aaaagatatg
    35761 tctggtttga aggtcaaatt agattggaca ggtataaaaa caagaattat tccatatgct
    35821 gatcctcaaa gtgaagtagg tacgactaat cgaatttatg gttcgccagt agatagtgcc
    35881 tatattaata actatcctga tgtctataca gaacatgttc agtttacaga agaacaagga
    35941 gtaaaagaca ttaagagctt aaataaaata gctaaaaatt actttaaaac gatcaatcct
    36001 ggctgtgata agcctaaagt ttctattacg gttgaatttg ataagttgac tgatagtgaa
    36061 gaagcgaaag aatttgcgaa gattagaaat tatggtctgt ttgatacatt taaaatatat
    36121 cataaaaaat ataatattta tcttgaatca aaggttagtg gactacaata cgattcttta
    36181 tctgaaaaaa ctttgaaatt agaagcagga gacattcaag ttgcttttta tcagcaacaa
    36241 gctgtaacta ttcaggataa attaaaagat tatgcaacga ataattatat gagtgatttt
    36301 aatgattatg tttcttcaat gattacaggt caaggcagtg caggaggata tgtagtttta
    36361 tggccaaaag aaaaaccttc caacatcttt attatggata atccagattt agaaaaggca
    36421 agagaagtac taagaatgaa taaaaatggg attgcttttt ctaagaaagg atggcggggg
    36481 ccttttaatt cagcttggac attagatagt atatttaatg ccaattttat acaaactgga
    36541 ttaatcaaag cagatatctt tcaaaattct tttaacaaga ctggagatgt attgaaatta
    36601 gttaatggac tactccaaat ttggaacaac aaaaagaaaa taatggagtt aactaaaaaa
    36661 ggaatggaat tttggaattc taagggttca attggaacga ttggaacaac tgattctgtt
    36721 ggtaatcctt ttcctggggc ttctactcca acccctattg aagataattc tttagttatt
    36781 cgtacaaatg gagacggcaa atatattttg atttctccta aagtaggaaa aggattagtt
    36841 ttattaggaa atggtaaagc aatttatttt ggagacttag atgtacaagg aaaacttaca
    36901 gttaaaggaa aagaaataat tcctggacaa ggtggtggtc catcgggagg tggagaatct
    36961 actggtgggt atcctagtga attaaaaaca gatgcagaaa agagagcttg gagaatttac
    37021 gatattttgt gtaataacgg a
//
LOCUS       DACJGB010000019        36568 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.019, whole genome shotgun sequence.
ACCESSION   DACJGB010000019 DACJGB010000000
VERSION     DACJGB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.019"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IYI34_001062"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IYI34_001062"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAR0558941.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IYI34_001063"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IYI34_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAR0558942.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVDCREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IYI34_001064"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IYI34_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAR0558943.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IYI34_001065"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IYI34_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAR0558944.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IYI34_001066"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IYI34_001066"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAR0558945.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IYI34_001067"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IYI34_001067"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAR0558946.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IYI34_001068"
     CDS             complement(8053..8523)
                     /locus_tag="IYI34_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAR0558947.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IYI34_001069"
     CDS             complement(8569..8994)
                     /locus_tag="IYI34_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAR0558948.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IYI34_001070"
     CDS             complement(9061..9330)
                     /locus_tag="IYI34_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAR0558949.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IYI34_001071"
     CDS             complement(9341..10936)
                     /locus_tag="IYI34_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR0558950.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IYI34_001072"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IYI34_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAR0558951.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IYI34_001073"
     CDS             complement(14489..15049)
                     /locus_tag="IYI34_001073"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAR0558952.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IYI34_001074"
     CDS             15402..16376
                     /locus_tag="IYI34_001074"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAR0558953.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IYI34_001075"
     CDS             16546..17946
                     /locus_tag="IYI34_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAR0558954.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IYI34_001076"
     CDS             complement(18024..18746)
                     /locus_tag="IYI34_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR0558955.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IYI34_001077"
     CDS             complement(18825..19331)
                     /locus_tag="IYI34_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAR0558956.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IYI34_001078"
     CDS             19454..21253
                     /locus_tag="IYI34_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAR0558957.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="IYI34_001079"
     CDS             complement(21321..22895)
                     /locus_tag="IYI34_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558958.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IYI34_001080"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="IYI34_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAR0558959.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IYI34_001081"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="IYI34_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAR0558960.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="IYI34_001082"
     CDS             complement(24672..25310)
                     /locus_tag="IYI34_001082"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAR0558961.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="IYI34_001083"
     CDS             complement(25311..25961)
                     /locus_tag="IYI34_001083"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAR0558962.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IYI34_001084"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="IYI34_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAR0558963.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IYI34_001085"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="IYI34_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAR0558964.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="IYI34_001086"
     CDS             complement(29104..29844)
                     /locus_tag="IYI34_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAR0558965.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IYI34_001087"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="IYI34_001087"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAR0558966.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="IYI34_001088"
     CDS             complement(31215..32153)
                     /locus_tag="IYI34_001088"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAR0558967.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="IYI34_001089"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="IYI34_001089"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAR0558968.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IYI34_001090"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="IYI34_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAR0558969.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="IYI34_001091"
     CDS             complement(35313..35618)
                     /locus_tag="IYI34_001091"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAR0558970.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IYI34_001092"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="IYI34_001092"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAR0558971.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9886 a         7988 c         6705 g        11989 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaatc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatcg cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaattg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gcaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ccagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 cgtcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc ttcttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc taattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgttcttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gatatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaata
//
LOCUS       DACJGB010000020        36512 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.020, whole genome shotgun sequence.
ACCESSION   DACJGB010000020 DACJGB010000000
VERSION     DACJGB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.020"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..311)
                     /locus_tag="IYI34_001093"
     CDS             complement(<1..311)
                     /locus_tag="IYI34_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR0558972.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     A"
     gene            complement(569..1846)
                     /locus_tag="IYI34_001094"
     CDS             complement(569..1846)
                     /locus_tag="IYI34_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0558973.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1925..2569)
                     /gene="pcp"
                     /locus_tag="IYI34_001095"
     CDS             complement(1925..2569)
                     /gene="pcp"
                     /locus_tag="IYI34_001095"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAR0558974.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2768..3712)
                     /locus_tag="IYI34_001096"
     CDS             complement(2768..3712)
                     /locus_tag="IYI34_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAR0558975.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3714..4400)
                     /locus_tag="IYI34_001097"
     CDS             complement(3714..4400)
                     /locus_tag="IYI34_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAR0558976.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4874..5194
                     /locus_tag="IYI34_001098"
     CDS             4874..5194
                     /locus_tag="IYI34_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAR0558977.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            5348..6253
                     /locus_tag="IYI34_001099"
     CDS             5348..6253
                     /locus_tag="IYI34_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0558978.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            6250..7023
                     /locus_tag="IYI34_001100"
     CDS             6250..7023
                     /locus_tag="IYI34_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR0558979.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7204..8628)
                     /locus_tag="IYI34_001101"
     CDS             complement(7204..8628)
                     /locus_tag="IYI34_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0558980.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8839..9285
                     /gene="nrdI"
                     /locus_tag="IYI34_001102"
     CDS             8839..9285
                     /gene="nrdI"
                     /locus_tag="IYI34_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAR0558981.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(9350..11332)
                     /locus_tag="IYI34_001103"
     CDS             complement(9350..11332)
                     /locus_tag="IYI34_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0558982.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(11346..12242)
                     /gene="murQ"
                     /locus_tag="IYI34_001104"
     CDS             complement(11346..12242)
                     /gene="murQ"
                     /locus_tag="IYI34_001104"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAR0558983.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(12245..13306)
                     /locus_tag="IYI34_001105"
     CDS             complement(12245..13306)
                     /locus_tag="IYI34_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAR0558984.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(13517..14044)
                     /locus_tag="IYI34_001106"
     CDS             complement(13517..14044)
                     /locus_tag="IYI34_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR0558985.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(14111..15613)
                     /locus_tag="IYI34_001107"
     CDS             complement(14111..15613)
                     /locus_tag="IYI34_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAR0558986.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15871..16992)
                     /locus_tag="IYI34_001108"
     CDS             complement(15871..16992)
                     /locus_tag="IYI34_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAR0558987.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            17118..18059
                     /locus_tag="IYI34_001109"
     CDS             17118..18059
                     /locus_tag="IYI34_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAR0558988.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(18209..19105)
                     /locus_tag="IYI34_001110"
     CDS             complement(18209..19105)
                     /locus_tag="IYI34_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAR0558989.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            19374..20714
                     /locus_tag="IYI34_001111"
     CDS             19374..20714
                     /locus_tag="IYI34_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0558990.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20932..21375
                     /locus_tag="IYI34_001112"
     CDS             20932..21375
                     /locus_tag="IYI34_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAR0558991.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21879..23297)
                     /gene="nhaC"
                     /locus_tag="IYI34_001113"
     CDS             complement(21879..23297)
                     /gene="nhaC"
                     /locus_tag="IYI34_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAR0558992.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23700..24923)
                     /locus_tag="IYI34_001114"
     CDS             complement(23700..24923)
                     /locus_tag="IYI34_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAR0558993.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24954..25793)
                     /locus_tag="IYI34_001115"
     CDS             complement(24954..25793)
                     /locus_tag="IYI34_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAR0558994.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25810..26490)
                     /locus_tag="IYI34_001116"
     CDS             complement(25810..26490)
                     /locus_tag="IYI34_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0558995.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(26492..27565)
                     /locus_tag="IYI34_001117"
     CDS             complement(26492..27565)
                     /locus_tag="IYI34_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0558996.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(28212..28883)
                     /locus_tag="IYI34_001118"
     CDS             complement(28212..28883)
                     /locus_tag="IYI34_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR0558997.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(28970..31648)
                     /gene="mgtA"
                     /locus_tag="IYI34_001119"
     CDS             complement(28970..31648)
                     /gene="mgtA"
                     /locus_tag="IYI34_001119"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAR0558998.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            32307..32744
                     /gene="ndk"
                     /locus_tag="IYI34_001120"
     CDS             32307..32744
                     /gene="ndk"
                     /locus_tag="IYI34_001120"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAR0558999.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32766..33632)
                     /locus_tag="IYI34_001121"
     CDS             complement(32766..33632)
                     /locus_tag="IYI34_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0559000.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33792..35885)
                     /locus_tag="IYI34_001122"
     CDS             complement(33792..35885)
                     /locus_tag="IYI34_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR0559001.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        10568 a         7664 c         6450 g        11830 t
ORIGIN      
        1 tgggcatccg atttatctgt tttccaactg cgtaaggtgc tttcttccat ctgttttttc
       61 gcctctaacg ggtttaacaa acagtaagaa aagtgatgat cttgaaagaa cttttcgact
      121 ggtctggaat aaatccccgt tgcttcaaaa acaatcaatg gacgctcagg caagtgctgg
      181 atctcttcga gtaatgcatg aaatcctctt tgagtgtgtt caatctcgtc ttcaaataag
      241 caggtgtcgt ctttgtatag gacactgtag ctgtgtccca ttgaaacatc taatgcaaga
      301 atgtaaggca tttgttttcc tcctttttaa gttttgaaag aactttactg cttcatttcg
      361 attccaattt cctatacacg ggctcatggc cccaacatac gtaaacctga ttctaaaatg
      421 acagtaaaga agttcggttt ttgataggga ctcgtagtcc tcaaggcttt gttcgaactt
      481 tctttcactc ttcactataa tctaaaaata gaaaatagag gagaacatca ttcgtcaatg
      541 atgttcccct ctaatcttag tatgtttttt atagtaattc cttcagatac tttcttgcct
      601 catcattgaa acgcagtgat tcttctaact tctgataata ttccaacgct tgtttctgag
      661 cttcagtaaa acgataatgt tttcctttcc atttgtcgat tgtctcttgc acatttccag
      721 attggatatc tttcactact agttgccatt tttcttttga gactgtacgg tctttcttat
      781 cagcagattg tatagcttct cgttctttac gcaatacttt taaaaaacca tttctgacta
      841 tccgaggaat gctgtcgaag aacccttctg taaaaccata gcgacaatat tgcttccaaa
      901 taaatagata gatttttggt tggatgaatc gttggttttt cgcaaaatat tccatctgag
      961 cagtgacttc tggtgttcgt tgacgttttg tctttctcaa cacggaaata aatgtttcaa
     1021 aataagcatt aggatgtact ggcacttctt catatacgat ggattgccaa tctttacgta
     1081 aaaatgcgcg atagttgatc aagaattctt ctacatcctt tactaattct tctgcaaagt
     1141 cgtcaaatag ttcttttttt atctgtcgct tttttgactg cccattgatg tgttcactta
     1201 ttttcttgat ttcattttcc aatgcttgat aatccgctat gtaaatcggc atttctactt
     1261 ggcgaaattc tgcgagccgc tgagctaatt ttatatccgg taaatatggc tgtttcatcc
     1321 gacgattttg gtacaagaca aaacagtatt tttgccatag tccttcagga aaatcgatat
     1381 tcttctgctt aagccataat aattcgtcta aggccagatc aacttttgtc attccctgat
     1441 ggatcgaagc tgccactgtc ggagagacat gcaggtgatc ggcaaagtgg ttgatgccta
     1501 gttttaggat ctttccggtt tttggcgatt taacgatgta ttgcactttt aattctctac
     1561 cacactcaca atacaatttt tgctggcttc gttttgttcg aaaaaacgga ttgatcttga
     1621 agtcagaaaa cacccaatct gttgcagcta aataatgttc ttccataaaa cgagagctga
     1681 ttagatatcg gcggtgctta tcgactagtt cccgctgttt tttcgttaat tttgcataga
     1741 tctcccaacg ttgactgctt gtgtattgtt tagaaatttt gatgggatag cttttgctga
     1801 tttcttttgg aaaccattct tcaaagacgg attgccattc ttccacggct gtttctttcc
     1861 tttctaatta caaaaagctg gctactaaca taacgttagc aaccagccag tgtttcttgc
     1921 taaattagtg agttgttccg gcatttccgg aaatatcttc tttattttct actgctgctt
     1981 caactgcata agtcaaagaa gaagcaatgt cttctaaaga catagaaggc gtacccactg
     2041 gacgatcgat cacttgatcg ggtgagaatg gcacgtggat gaacccacct cgaattgccg
     2101 ggaattgtgt ttcgatcaaa tgaagcaaac gatacatgat atcattgcag acaaatgttc
     2161 ctgctgtata agagatgtac gctggtagac catgatcttg tacatttttt gtcattgctt
     2221 tgattggcaa tgaggtaaag taagcggctg gaccgttttc ttctaaagcc gtatcgaatg
     2281 gctgatttcc ttcattatca gggattcttg cttctgctag attgattgcg acacgttcga
     2341 acgagactgc tgaccgccca cctgcttgtc cgacacagat cacgacatct ggttggtgct
     2401 gttggatggc ttctttgact acttgactgc ttttttcgaa tacggtcggt acttccactt
     2461 tgatgatttc agcaccgctg atttcatctg gcattttttt tactgcttca taggctggat
     2521 tcactttgtc cccaccaaat ggatcaaaac ctgttactag tactttcatc tgttgttcct
     2581 ccgtccgatt gatttaacat atggttatct tttatcatac cattttcctt gaacagcgta
     2641 aataaaggaa acaatagttt aaataacata acagatcaaa cttaatatat tcttataaaa
     2701 aaatagtgtt ccactacaat gttagaagag gttgcgacaa ttattctgtc acaacccctc
     2761 tcttctatta tttaaataga atcatataag caatttggaa aactagcata aataacgcaa
     2821 taaacaactg gtttttgatc actccgtatt tgtctttcat ctccagcatc gctacaggca
     2881 cgatattgaa gttagcagcc atcggtgtca gcagggtccc acaaaaaccg caagtcaatg
     2941 caaccattcc tattaacgct ggattcgctc catgactaaa tacgaatggt gcaccgatcc
     3001 caaccgtcat cacggtgatg gccgcaaatg cattccccat gattaccgta aatatcacca
     3061 tgccaagagc gtaaatgatg atgccgatgt tcacatttcc ttctggaacg atcgtaccta
     3121 ctgcattgga aatcactgtc ccaactccag cgcttgtaaa taccgcacct agagaagcta
     3181 aaagcattgg caacatgctt aacggcccta ctgtgcccag catatctgct gcatcatcta
     3241 aaaatgtact tggtcgatta tctcgagaaa agaacatcaa gattagcatg gctgcaaaaa
     3301 caccgacgcc cacgccgact aaagcaccta aattggtaaa tagagcaaac aagatcgcaa
     3361 agacaccgat actcaaagct ggaataaaaa ttttcattcc tagtttgtct gacatttttt
     3421 ccatatattc ttttgatgga agccggcttt ctcctttgct gactttctta gcaattgctg
     3481 gtatggtcat cgcaaagatc aagccaccgt tcacgattgc cggaagccat cttcctcctg
     3541 caataactac ccctaagacc gtccagaaaa aggctgaccc ataacgatga ggattggttt
     3601 tatctgttaa atttcttact ccagtgtaaa tcaatacgag tcccatcacg atataaagga
     3661 tctccagtaa tttatcgcct aataaaatat caggatttgt aaaaaagtcc atcttattgc
     3721 ccctttcctg gataatattt cttcgctaat ttcttttctt taagatagaa gtagacactt
     3781 gccacaacta aagccgtgat agccactgga acttccacta aagcaagctg tcccaatgtt
     3841 acgtcatatc ctagatcttt cagtgttgat tggactagca acgcccctgc tcctccgatg
     3901 aatagtactt ggccaaagaa ccagcagaca ttttccatag ctgaagccat accttttagt
     3961 tcttcttcat gcttaggatg gacttctaac ccttttgatt cgatggtccc caatgccatc
     4021 ggtaaaatga ttggacgaac aaatccagca actccaccaa aactcacgtt aaaagccgca
     4081 aagatcattc gaaagatccc ataaatatct acaatcatac cagcagatac tttcttgaat
     4141 cgcttgatca aggaagccgc tgattctttc agaccatttc tttcaagcgt tcctgttgcc
     4201 aacataatga tgataaaaat cgccatcccg cgattgttta caaaacttgt acctaaaagt
     4261 tctaacattc cttgaatgcc aagaccgcca gtcaaagctg taacaactaa agcaaccatt
     4321 acgatcaata tcgagtctaa tttaaatgca aacccgagaa taacaatcaa gacgcctaaa
     4381 aggctaagat aattttccat atattttcct ccataaaatg acaatatttt tttaattata
     4441 aagcaaaaca cataaaaaat atagttgttt tctcacgaaa tagacactat ttttaaatat
     4501 ttttcgttat ttttttatca caaaaattat cttacttatt gtaaggtaaa gcttttttta
     4561 atttattgcc cgataatata tagaaataag gtctagttac tgtttttttt aaatgattaa
     4621 taaataacaa cttctttcta tgaaattgca aagcattcat cttcttttcc gctaacgata
     4681 tatccgtcag tgcttggtac actttactaa aaagtatgag attgccgata tctggatagg
     4741 tgtgctatac tacagaaaaa gatatacaat ctgccaccgg gtaggataaa gaggatgatc
     4801 attccattag gtctttgaag ggatgatttt cctctttttg tgtatttttt tatagttaag
     4861 gaggaatgta agtatggcat ggatggaatt aatcatagca ggaattttgg aagtattctg
     4921 gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc aattattctt tttacacagt
     4981 gattggcatg attgctagtt tttacttttt gtcaaaggca atcaaaagtt tgccgatgag
     5041 tctctcttat cctatctgga cagggattgg cgcagttggt tcggtcataa ttggtgtggt
     5101 cttgtttcat gataagctga atctttccac ttggttcttt gtggggatgc tactcatcag
     5161 tatcattggc atcaagatta ctagcggcca ctagacactt ggaataagta ctgatcagaa
     5221 aaaataagcc tttttattta atattcaatt tttttatgaa aaaataagaa ataagtccaa
     5281 tgacctagcg acttctccac tcatcaggtg tatgcttgta ctatctatag aaaaggagtg
     5341 aatatttttg aaaagtcttt cggtccaaga attatccaag aactttggtc acaaacaagc
     5401 cctcagcgat gtcagtttta gcttatcccc tggcaaaatc gttggattag taggtcctaa
     5461 tggcgctgga aaaacaacga tcatgaaagc tattttagga ctgatccatt actcagatgg
     5521 aacaatctgt attggtgaaa atgagatttc tccctctagt cataaagggt tggaacaagt
     5581 aggagcattg atcgaatacc cgggtatcta tccattctta acaggctatg atcatctgaa
     5641 gttattctct gaaaccaacg atgtatctgc tatcgacact atcgtgaatc agctcaagat
     5701 ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg ggaatgaaac aaaaattggg
     5761 aattgctctt gccttactga accatccgtc tatcgttatt ttagatgagc cgatgaacgg
     5821 tttggatcca caagcaacaa gagatgtgcg aaaaatcatt accgacttgg ctactcaggg
     5881 cacctctttc ttgatctcca gtcacttact cagcgagctt gaaaaaatgg tcgatgagct
     5941 gatcctgatc aatcaaggaa agatcatcaa acaatgtaca atggctgaat tgaaccaaac
     6001 agcgcaagat ttcatcgtta tctctacaac aaatgatcta gctgctcgaa cgatcttgac
     6061 agaagctgga tatactttgg aagatcctga taaaatcaag cttttaaaag aaaacgaaga
     6121 attattagga accgtgatcc agctattgtc tgcacaaaat atccatgtga cagacgtcca
     6181 gcatctgacg aatgatttgg aatcttctct attggaaatt ctggattcag taaaggggga
     6241 agaagaaaaa tgacattgct tgtaaaacaa gaattattta aattgatcaa gaaaaagtca
     6301 actgctgttt tatctgttct attagttgtt ttgctcatag gaacagcact tctagccaaa
     6361 aaatacacga cgatcattga tccagttgaa atgactgctc agctttttag tgccacatct
     6421 tggattgtct ttatcatgat tgcagctgca agcacgatca tttcgatgga agcacaatat
     6481 ggtacattga aaaatctcct ttatcgaaag tattctcgag gagaaatatt agtaagcaaa
     6541 tggatcacat tagttattta ttctgtttat ttataccttt tagctattat tgtcactgtg
     6601 ttgatgaaac tgattctttt cccttcgatt tccttcacgg agaaggtttc tactggtcaa
     6661 acactgatcc aaagcttgtt cacttatact ttgggtagtt atatcggttt atggctgatt
     6721 ttaagtttag tgttgatgat tgcttgcttt atcaatagtt ccggcgcatc catctcagca
     6781 ggaatcgtct tttattttgc ttcgtcaatc atatctggta tccttattgc actcatccaa
     6841 aaatgggagt ggatcaaatg gaatccgatc agtatgttga atctgcaaaa ccaaattgga
     6901 aacgaagaga tcatgaagca attgactcat ctttctacta atcaaatgct attcggtaat
     6961 ttagcttaca tcgttttgtt cttagcatta ggatatctcg tatttaagcg aaaaaatgta
     7021 tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca gccttattgc cgattggctg
     7081 tctcttaaac accgtttatt ctgaaaaagg agctgtgaca acatttgtgt cacagctcct
     7141 ttcctagtat gtttttgaat aaacgacagg ctcacaacct ctctttatac ttcttttata
     7201 cttctatacg tgaagcatcc cagtggcaaa taaaaccatt gcaatcactc cgaccatcgt
     7261 cacacctata agtaaaatct tatctaagtg gatatgttgt acaggctcgt tttgctcctt
     7321 acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga acaagaacaa gcattacttc
     7381 attaaaggct acgactagca tagcccatac ttgaaaggca cttgctaaaa gccctactaa
     7441 aaattggacc cattctttcc gctgataaga aagtttcatt tggtacaatc ctaagaaaag
     7501 ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca aagttataag cactgtcagt
     7561 gaataataat gaaagtaaaa agacattcgt acataacgtc gtcaatacga tagcataagt
     7621 tggtgcccca tttttgttga gttttcccca tgctttaggc aaaagtccgt cttcagccat
     7681 gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc caaacaccaa ctaaagaaat
     7741 gatcaaaccg ccgttgatca cgactgctcc ccatttacca accatcattt caaacacata
     7801 gcccatagca ggctgcgcct tgatcgtttg cagctgattc atcgtcattg tcccatatgg
     7861 aagcatggaa accaagacgt aaataataat caagccgaat atccctagaa tcgttgcttt
     7921 cccaacatct gattgttttt ttgctcgtga acctacgaca gaggctcctt caataccaac
     7981 aaatacccaa agcatcacca tgatgcaact caatacttga ctggaaaacg ggatcggctg
     8041 tccattcgca ttcaatataa tgtcgctaga aaagttatca atgaattgtt ggatcgaaaa
     8101 cgtgctgata gctactacaa taaataaaaa taatggcact aatttacaga atgtgacgat
     8161 agcattgacg aaactagcat tttcgactcc ccgattcact agccatgcat atccccataa
     8221 aaagactgta ctcatcacga tcgatgccag attttgccca ccttcaaata ctgggaagaa
     8281 atatcctaat gcgctcatca acaatgttgc aaaagctacg ttgcctaacc agcatgtgag
     8341 ccagtaaccc caaccgctga taaaaccagc gaaacggcca aaaccttctt ctgcgtaact
     8401 aaagatcccg ccttttaaat ctggacgttt tttattgata ttattgatcg acaaggagag
     8461 catcaacatt gctgttccta cgagtaccca actgaaaata gctgggccgc cagccgatcc
     8521 ggagatgtcc gtcataatac caaatacgcc acctccaatc gctgaaccaa ttactaaggc
     8581 agcaagtgga attaaaccaa ttcctttttt ctgttgctct gtatccatgt aagcatcctc
     8641 caaaataaaa ccgcttttat caaaaagtat aacaaacctt ttttattttt acttaattaa
     8701 taaaaacttt aatagaaata taattctacc ggcttttttc tcattataca agcaaagcgc
     8761 ctagtccctt tccaaattac accaccaaaa aacttaaaaa agatgctata ctaaaaggaa
     8821 agaattggag gatgatccat gaacatttta tatatatcga tttcaggaaa tacgcgtgcc
     8881 tttgccaaac atctagcaga atatgctgaa aaaatgcatg cagaagatcc tgtcaatcct
     8941 gaagttactt taaaagaaat ccatgaaaat agtgatttta cgaaagaaac agaaccattt
     9001 tttactttcg ttcctactta tttagacggc ggaaatggtt tagataacgg tgatacagaa
     9061 atcttgaccg aaacgatgcg tgaatatctg gaacatgaag acaatcataa actttgtctt
     9121 ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt attgtctaac agccaaacaa
     9181 tatgcacaaa catttggctt tcctttcttg gcagattatg aattacgggg aacaccatct
     9241 gatgtgacac gaatttatca gattttagca gaaaacaata aataataaaa aagaagaagc
     9301 tgaaaaatag atttcagttt cttctttcgt ttctattgtt tctatcatat taagctttga
     9361 catccatcac aggtgctttt gcttttgctt tacctgtttt ttccaagaca aagtttttta
     9421 cacgatccat attggttacc aagacgatca tggatgtttc tttgcctgct gctttgatcg
     9481 cttctaagtc agcttctgct acaagcgtat caggtgtcac ttgctgccct tctgttactt
     9541 tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat tcccatatga agcaagattt
     9601 ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt tgggaagaca gagctgacta
     9661 ccccagtaac tggagaatag atatgtccgt tttctggttc tacaaagtag ccttcaccca
     9721 tcatcttttg tgcaaaaact ggatcacttg ctttatctaa agaaacaact gttccgtcag
     9781 caactgatga gaggatagtc acactttcag ctgaagtttg aggtactact ttttcaggca
     9841 cttcatttac tgtggctgtt tcagtcgcta attcttcttt ttctaaacgg tctttaggaa
     9901 taccgaagaa gaatgtagca acgaagccac cagcataagc tgccaataat ccaaggatgt
     9961 agcctaacca tttaccgtca gagatcaatg ggatcaaagc ggctcctgaa ggtccaatag
    10021 cgatagcccc gacatttccg atcattccga tcactgcccc gccgatacct ccaccgatac
    10081 aagcagtaat aaatgggcgt cctagaggta atgttactcc gtaaatcaat ggttcgccga
    10141 ttcctaggat cccgactggt aaagctcctt tgatcatttc tgttaattct ttatcttttt
    10201 tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc tgcaccagcc atcgcaagaa
    10261 ttggaagcaa tagcgtacga ccagtttcat caatcatttg cacatggatt ggagtcaaaa
    10321 tttgatgcag tccgaacata accatcggca agaataatgt gcctagaacg aagccggcaa
    10381 atgctccacc aacgccaagt acccagttga tcccaccgac catgccatca gaaatgaaac
    10441 ctgctaaagg catgatgaag aaaatttcaa tcagaccgat ggcaatcaat gaaagagttg
    10501 gtgtaacgat gatatctacc gcatcaggta ctactttatg cagttttttc tcaacgattg
    10561 acaatagcca aacagcgaag atgacaccta ggatcccacc ttgtccagct gcaagagctt
    10621 caccagtaaa taagtttttc agtggtgctt ccggattcat ccctgtcagc atcacaacgg
    10681 ccccgataac tccaccaaga gttggtgttg caccaaaaac ttgtgcagca ttgatacctg
    10741 tatagataac taggtagcta aacatcccgt ttttcaagat gttcatcaca tcgatatatt
    10801 gttgccatgt tgctgcatca agtgttccgg ctgtgactaa gtttgctaaa actgcggcaa
    10861 tccctgccac gatcccagta ccgacaaatg caggaatcat tggcacaaag atattagaaa
    10921 tgtcttttaa taccgatttg attttagatg gtttttgttt cgatttttgt gccgctttca
    10981 tttcttgtgc ccgttcgttt acacggtctt ttccagatgc tgatgaattt gctgtagctg
    11041 tacttacaga agaaagtttt tctcctaatt tcacgcctgc caaatcaacc atttgctgtg
    11101 ctactttgtt cactttccca ggtccgatga tgacttgaag ctgttcatct tctactacac
    11161 caagcactcc tggaatcgct tttaattcag agacatttac aagatcatca ttgatgatct
    11221 tcatacgcac acgagtcatg cagtggacaa tgctttctac attgcccatg ccgcctgccc
    11281 ctttataaat ttgttttgcc agttcttcca aagtcagttc gttttttgcc atggtctttt
    11341 ccccctcata aggtcccttt tacaaatccg tttgcttcag ctaattttgc ttgagcttcc
    11401 tctttatcac tatttgtcaa aatcatgacg atagccagct taacattttg atctgcttct
    11461 tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg cttgcatgat gattcgtttt
    11521 gagcgttcga ttagtttttc atttgttggt ttgacatcga ccatcaagtt attgaatact
    11581 ttacctatac caatcatact gatagtagaa atcatattca agataagttt ttgtgctgtt
    11641 cctgacttca atcgagtaga gcctgtcaaa aattctggac ctgcatcgac ttcgattggg
    11701 aaatccgcgt gtttactgat ttcggcttgt ttattgcaag aaatcgttcc tgtgacagca
    11761 ccgatcgatt ttgcgtaatc taatccaccg atcacatatg gtgtacgacc gctagctgcg
    11821 ataccaataa ctatatcatt ttttgtcaaa ttcagcttga ttagatcttc ttgccctagc
    11881 tcataagaat cttcggcccc ttcgacagct acagtcattg cttcttctcc accagcaatt
    11941 agacctatga ccatttccgg ttctacaccg aatgtcggta cgcattcagc agcatctaac
    12001 acacctagtc ttccacttgt tccggctccc atataaatca gacgtccacc ttttttaaag
    12061 gactcaatcg tgttagcaat cactggttcg atacttgtca gttctttttc aaccgcagcg
    12121 gccactttct gatcttcttt gttcattagt tgaacggctt gagaaatact catctcatct
    12181 aatccaaaag tttcttggtt tctgcgttcc gttgttaggt tctctaaatt gattttgctc
    12241 atttttatct tccttctttt cttataaatt gatagttcat accagctttg atttggttga
    12301 tcaatggtag ctcttctttg ataactttag ctactacatt cactttttca tccgctggca
    12361 aatcatattt ggttaattgg atctcaccca tatagcgcaa atacttttcg ttatctaaag
    12421 taatagaccc tttcatacgc gtagctgtat ttcttgctgg gataggcgga atctctcgaa
    12481 aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc ttgccgattc gtatgcttcc
    12541 ctaatatata ttcgtaaaat tcttcatcta caggttccgt atggagcaag actgtctgtt
    12601 ctttttggaa agaagcaaat tgctcctgaa cgttttctga cagacctgca tctccgatgt
    12661 aaacaatatc cgtgttactt gctaatagat ctagagcgcc tgcaagagga tggatccctc
    12721 gatgttcttc caaagttggc aatccttgat atagtgggcc tctcaactct gtatctcctg
    12781 gtacaaaacc ttggacaagg agaccttgaa ttttgagcca ttgatttttt ttctgatacc
    12841 attctttgtc cagccctgtt tcgggacgtg gataataatt atgccaagct tcaatctgtg
    12901 agaaatcagc ttctgcttct ttcaattcat cgatatcttt aggcgtgatc gtgctagcat
    12961 tcaaactgat tttcaattga tgtgaccagt cggcaatctg ctgattggaa atatgataat
    13021 ccattcttaa acctgttaca ccaattgctt tcaaaggcgt tagatcttct aatgaaaaac
    13081 ccgctcgttc caatgcctct cctgaaatat cgaccatcaa ttctagttta ttctttttcg
    13141 cttcgcttcc tagttcgatc aatcgctttt gatatgccga aacatcatct tctggtatat
    13201 gcatggaagt gaaaataccg acaaagccat tggatgccat ttggcggata tactgtttct
    13261 tctcttctga caaagaatcg ttcataaata ttgaaaagcc aaacatatta tccctctttt
    13321 tctgtatccg tcttcatttc tatgactaga gtatatatct aaagaaacaa aatttcaata
    13381 cttttattaa aataattaaa tttcatttct aaaggttgag tttatattat caaaagaatt
    13441 gtcataacct aaaaaaggca ctgctaagca aaagcagtgc cttgaaatat tttatttaaa
    13501 taacacaaaa acataattac tcataagaaa ccaattctgt attatctacc atgaatgtaa
    13561 agaacccctc attaggcatc ggatgaaaat aagcattgat cactcttatc aaggcattat
    13621 ggcaaactaa cagatagaca ttttcttcgt cttcgatgca ttctttcaat aaaggaacga
    13681 tccgtgcata cacatctaag actgactccc catttgggaa tcgtacagcg aacgctaaac
    13741 gtgctttttg aaagttctca tctttgcttg gcagtccatc ataatcccca aaatccattt
    13801 ctaccaatcg ttcatcaacg gtcagcggca agctgagacg atcagcaact gcttgtgctg
    13861 tatctcttgc tcgttgaaga ggggaatgga tgattttcgt gattggaaca ggcaaatcta
    13921 ctaccttttc tgctagtttc tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg
    13981 cccgtccgca aactaatcct tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa
    14041 gcatgtgatt ctctccttcg ttggcaaagt tccccttaac cataccttgt tgttcttgag
    14101 attacaaggg ttagattctc tcaagagaaa taatctcagt agatacttta tccaggaagg
    14161 tttgatcatg ttccactagt aacatggcag gtttcacgtt caagatcagt tgttctaatt
    14221 gttcttgatt gaagacatcc aaataattca atggttcatc ccatgtatat agttcagctg
    14281 gctgtgataa agatttagcc aattccactt ttttccgttg gcccatactc atctgctcga
    14341 tcttgttatg aaaaacatct ctttccatcc caagctttcg gaggttgttc aaaaatgctt
    14401 ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt tcctcgattg tcttcataat
    14461 tttggcttgc ataactaatg ctcagatgtt ttggctggta tttttctcct ataaccttgc
    14521 cgttgaatgc ccccagaaga taatggataa tggatgactt tcctgcaccg ttaggacctg
    14581 aaatcgccac ccgctgatga ggctcgattg taaaatgaat tggctcgaat aacagatttt
    14641 cataccctaa ttgaagatct tctacgctta aaagtcgctt atggtgagac gcttggctat
    14701 tcatcgtcaa agaatcgata tattcgatat cttttaatag tttttctttc tcgctgatct
    14761 gggtctccat ccgattcacg attgctttgg atcgtttcat cgtccgtgca gcatcagcac
    14821 ccactgctcc tttattcact cgtctagatt cagtatcgat gaatccgact tgtttctttg
    14881 ttttatctcc ttctcgggaa cgagaccatt cggctttttc agctgctgtt ttctttagcc
    14941 tactgacttc ttttttcaat ttttcatttt gagccatttc aaattcatca cgaagttttt
    15001 tctgttcttc atagatagag aaattccctt gataaagttc cagttgactt ttttcgattg
    15061 ctaaaacatg gtccactact tcatcgataa atccccggtc atggctgacc acgatgaagc
    15121 cttgtttttt ctttttcaaa taagccgcta cttgttttct accagagata tccaaatgat
    15181 tcgttggttc atcgattaac gggaaatgag tgtcatccac aaataaaagt gccagtagga
    15241 cttttgtctt ctctcccccc gatagtgtgc tgaattctct ccataagatt tcaggatctg
    15301 tttgcatcaa ttggagctct ctttcgattt cccatatctc aaaatccgta atatcattta
    15361 acacgtaata ggttaaacgt tctttatctt ttgtctgttg cgggaaatag gcaaattctt
    15421 gctgatggat tacttgccct tgataaggta gtttgttttg tagaatattc agtaaagttg
    15481 tctttcctcg accgtttcgt ccgataagtc ctagtttcca ttgtgtgtca aaatttagat
    15541 ttgcttgttc aaataataat gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt
    15601 cgattttaga cattttccat cacctcgtaa aaaaagacca gtcctatttt cagaactggc
    15661 taaagaggat gcaagggttg gatacacaca aaaagaccca tacatcgaaa aaaccaagcc
    15721 aatcccgaaa atctgcacac tgcttccttg tctccacaag gtacagtctg aatgcagctt
    15781 tcatcaatcg acttaatttt tcaatgcacg gatccctcac tttgtcctat atttgaaatt
    15841 acgatagcat aaagaaaatg attttgtcaa ctacttcggg aggatggtga aaaatctgct
    15901 aagtgtctgt cctaagattt ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc
    15961 taaacctatt ggacccaaat gaatacttaa gacaattggg agaaaatcag aaagtccttc
    16021 tagctcttgg tctctacttc cgcaaatagc gattattggt tttcgatatt tttttgcaag
    16081 cactgctaca ccgtatggaa cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc
    16141 tgtaatgatg acatctgctt gttcgatctt tttttccaat ccagtgattt ggctgactaa
    16201 atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc
    16261 tgctccactt cctggaatat catctaaatc cacctcccag aaatctttta tttgctgagc
    16321 aatctttcct gcttgtttat ctaagaaagc tagcgtcgta tctattcctc ctttttgtct
    16381 tccaaattgc caagcagctc cttgttgacc tgtatatgga ttggtaacat ctgcagcgat
    16441 aatcaatttt actttagaaa caagaacgtt tcttcgatca aaatgaagtt ttttagtaga
    16501 taataaagga tttccttctg tgtacccttc taaagaagca cccaaggatt ggagtaatcc
    16561 tagaccacca tcgctacacc cgcttcctcc taaagtgaca ataatttgtt ctacttcttg
    16621 aagcagtgca tctttaataa gttctcctaa ggcataactg gaagcaagtc ggatagtttg
    16681 atccgttggt ttatcaagta gatctaaccc caatgcttgt gcagattcga tgactgcggt
    16741 gttttttcct tgccaagttg tcaataaata gctgattttt cgctgacgga ataagagatc
    16801 catcccatca atggtacgcc atgttcccct gagcgcgtaa tagattgctt ctgcagtatt
    16861 ttctccgcca tctgcaatcg gcacattgat ttttcttcct ttacgatctt cccagccatt
    16921 tagaactgcc tgatttagtt caaatgaaga agcacttcct ttaaatgaat caattgccgc
    16981 caaaatatcc atctgtatcc ctcactttct tcttaagtaa gacacttttt ttcatttttt
    17041 tcaaacaaaa caatgctatt ttcttccatt tttctatata ctaaaaggga actcattgaa
    17101 taaaggagag aaaaaacatg aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa
    17161 tcggattgat gctacatcaa agcggaaatg aggtcctctt gatcgatcgt tggcctgctc
    17221 acattgaagc cattcgtaca aatgggttga ttgctgactt taacggtaaa gaagtggtcg
    17281 caaaactgcc aatctattca ccagaagaaa tcatcgaaag caacgaacac gtggacttga
    17341 tcgtagcatt aacaaaagcc aatcaattag atgatatgtt ttgttcgatc caatccatta
    17401 ttactgacaa tacttatgtt ttgtgtttac taaatggatt aggtcatgaa gatgtactgg
    17461 aaaaatatgt gccaaagaaa aatatcttat ttgggatcac gatgtggaca gctggtctag
    17521 ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat cgaactagag aacttggaac
    17581 cagaaggcga agcatttaca aaaaaagttg tagaagtctt tcaagaagca aatttaaatc
    17641 cgatctatag ccataacgtt cgttattcca tttggcgaaa agcttgtgta aatggaacat
    17701 tgaatggttt atgcacgatt ttggattgta atattgctga actaggcgcg caaaaagcgg
    17761 cagagagcat ggttcgaaca atcgtatctg aatttgcttc tatcgcagca aaagaaggaa
    17821 tcatactaga tcaagaagaa gtgtaccaac atattgcttc tacttatgat cctgacaaca
    17881 tcgggttgca ttacccatct atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa
    17941 tcgactacat caatggtgcc atttggcgaa aaggacaaaa atatgatatt gctacaccat
    18001 attgtgcctt tttgacccaa ttagtccatg caaaagaagg aatccttggc gcagaataaa
    18061 aggctctctg atgagaatca gctgaatcag cagctggttc tcttttttta agcccaataa
    18121 aagatcaaaa agaaaacaca actctcatga tgccataaga attgtgcttg tttgtttcag
    18181 tacataatct cttcaaaatg taggtttttt atttgttttc aacagaggag aggtcatatt
    18241 cgtattgctc ataaatagtg gaatcgttag tgataaatcg ttttgcccaa ttttctgcat
    18301 cataaaattc caatagctct gataggattg gcttaaatgg tgtatgcaga aattccggta
    18361 attcatctaa atcacgttct cgaagcaaaa aaatattttc aggacgataa aaatgataat
    18421 gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa atacatggat tcaaatgtcc
    18481 gcaaagtcac tccttgctga ccctcccgca acagttccag aatcccgcgc gattcccctg
    18541 tttttttcag gtactcttca taagtaagat atccaggttg ttcattccct cgatgtggaa
    18601 gaatgaaata gttggtcgta acatctcttt ttataaattc ctgcttatag ctttctgctc
    18661 gttcttttct cccattattc gttgcaccaa aaaatagatc cgtctttggt tcgctagaag
    18721 aaaaattcat atgtttcgaa taaaatgaac tattatgttt cagcccaagt tcttttgctt
    18781 ccaaaaaatc aaaatgataa atctcatcga ttgccggttc atcttgtgcc tgcttcagca
    18841 tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa cacaattttt gcattggtaa
    18901 atgggcgcac gcgtcgagcc atctccatat caaatccata atctaaaaaa acgatcagct
    18961 gataatttgc caactgttgt ctagctgatt taatgaattc cgactgtagt aaatcttttc
    19021 cggaccaaac atctaaagag cggatggaag gcaaaaaata aagtgattta ttttttgcaa
    19081 ctagcagtac gtcatttttg ttcatctttt ccgcctcact ttctctgtca ctttttccta
    19141 tgatagcctg tttaaatagt aaaaagccag taaaacgttt cctatatttg tatgaaaatt
    19201 taacatttgt atgaaaatat gatgaaataa atcattcttc tattaaaatt cgcctaaaag
    19261 ttttctgaat agttgtaatt gtctgaaaat ttgttatcat tagtcattgt taaatattgc
    19321 tatttattct taatataaat tttgattgtg aatttaaaag aaagaggtca tttatggaag
    19381 ataaaaaatt gaaaaaacaa cttagctcca gacatatcac catgttagct ttaggtggag
    19441 caatcggtgc aggtctattt aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg
    19501 tattgattgc ttttttgatt ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa
    19561 aaatcgtttt aagcggtggc gacacgcatc acggattatc tggtttgata cgcccttatc
    19621 taggtgctca ttctgctgat tttactgatt gggtctactg gtctatgtgg attatcaata
    19681 tcatcgctga agctgttgca gctgcttcct ttttgcagtt gtggttcccg cacattccag
    19741 cctgggtatt tgtttttatg ttggctgttt taactacgat catcaatctt tattcggtac
    19801 ggttattcgc agaaacagaa tattggctgg cttttgctaa aatcagtgtc attatcttac
    19861 tgatcatttt tgctacttat cttgtcggtc agcaaatgtt gggcacaggc gttttcccta
    19921 cccttcagca attgactgac catggtgggt ttactcctca tggtatgaag ggaatcatca
    19981 gttctttgct tgtagttatc tattcttacg gcggttctga actgattgct atcacggtaa
    20041 gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa
    20101 tcatttcctt ttacatcatt cctttgtttt tgcttttgat tctttttcct tggaatactt
    20161 tagcaggcac gaatgtcagt ccttttgtga tggtatttga aaaaatgaat atcccatttg
    20221 cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg
    20281 tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa
    20341 aactagctgt tttaaacaag catcaagtac cgcaacgtgc tgttttattt tgtaccagtg
    20401 ttctttatct tggtgtagct ctttcttatt ttgtcggtga caaattattt ggctatctgg
    20461 caggttccct ttcgtataca gtattattga tctggatcat tatcagtgcg gcatcctttg
    20521 tactggcact aagaaaagga acattatggg ataaaggaat cagctttttt gcattagctg
    20581 tcttaggatt gattttcctt ggtatcctgt tcacgaactc cattggggta actgtattga
    20641 ctggcctgct ttacctgttc atttatttca gttatcaaaa aaagaatgat gcgttcgtat
    20701 tgacgaatga atgaaatcga tagaaaattt tattttattt atgtggaata gatggagcta
    20761 ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc tttttccgaa taaacagtct
    20821 catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc aaaataattg attgttctcc
    20881 atgctcatga catgatagaa gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag
    20941 tattggaagt ttttgttgat gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac
    21001 ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta cacgagtatt catggcgttg
    21061 atgatgggtg cagtcatggc agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac
    21121 aaaaagataa atgttatgat cttagccgct agtcttcttg tttttgccgg ttccttgttt
    21181 atggtacgaa gccaaactac tgtcaacgac acagcttgga tgaaggccat gattcctcat
    21241 cattctatcg ctatcctggc aagtaaacga gctgatttaa aagatcctga agtaaaaaaa
    21301 ttagcggaag aaatcattca ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc
    21361 aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag aggatgttcc agaagttatc
    21421 agcttcaaga aataagaaga agctacttct ggaacgccgt gaataagggg ttgtgaaaaa
    21481 gctgccctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcct atttttcgtt
    21541 tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattaag aggttgtaaa
    21601 atcagcgttc tgacctgagt attagagaga caaatggaaa aatagttcct catattttgc
    21661 cattttttga gctaataccg agggtcagct gattgaacac cgtttatcga gaggttgtga
    21721 aaaagtcgct tagtttcgcg tattaagaaa aattttgtga aatggctttc cacatttttg
    21781 tataaatttg gcttaatacc gagaaactgg cttttgaata ccgtttattc gtaaaaggga
    21841 ttgtgacaaa agtcttgtca caatcccttt ttatgttttt attgttctaa ttgcgctgtc
    21901 ttgcttactg cttcttgcgg tttcacaaat ttttttgaga ttccccaacc tgtgaaggca
    21961 ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt
    22021 gtaggaatac caaatgtact tgctacgaaa cttccggcaa ttccccaagg cactaagtag
    22081 ttgattactg ttccaccatc ttctagcgtt ctacctaata cagttggatc aaatcctgct
    22141 ttttcataag tttctttaaa tgcattacca ggcagaataa cagataggaa ttgttcacca
    22201 ataaaaatat ttactccgat acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc
    22261 gtcatctttc ttgatactcg ttccatcacg gttgagatca agccaaattc gatcaacaat
    22321 ccacctaatg acaaagttag aataatcaat gacacagtag gcatcatact ttcgattcct
    22381 ccgcggctaa gcagtgaatc gatttgttgg tttccagtat ttgaaacaaa ccctgattcg
    22441 atgaccccag ccaacttcgt taaggaagtt tgtgggtttt gaataaaaat cattcctact
    22501 gctacagcga tattcaacaa aatcgttaaa attgccggca cctttttcca tgcacacagt
    22561 aacattaaaa ataaaggaat cagtgaccag attgaaatag taaagtttgc ttctagaaca
    22621 tcagtcactt cacgaataga tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg
    22681 aataaaaaga aagaaacaac aaatgccggg acagttgacc acatcatatg cttgatgtgt
    22741 ttgaacaaat cggcatcaac aatagctgct gctagatttg ttgattcgga taatggagac
    22801 atcttatctc caaaaacggc acctgaaaca atagctccaa caaccagcgc tggatttaag
    22861 cctagtgttg tcccaatacc gaaaaatgca atcccaatcg ttgacataac agtaaaggca
    22921 ctccctacag cgctacctac gattgcacac acaacaaata cagaagggac aaaccactgg
    22981 acactgatca atttgaaccc aatgaccatt agagttggaa tgatccctgc ttgtatccat
    23041 acagcaatca atgaaccgac taaaataaaa ataaagattg ggatgattcc tggtttgatg
    23101 ccattgatga ttccttcgtg cagccaatct gtcgggtatc tttttatcac tgcatacaac
    23161 atgaccattc cgatcgctgc taagatcgtg atattaggtg agacctttaa tccgacaacg
    23221 ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg ttgcttctaa aaaacttggt
    23281 gttccttttt ttcccatgat aatctctcct tttttagttg ttcttatgtt ctaaaaaagt
    23341 taagatatcg gattactccg ccgcaattca tccgcgattc ctcctttata aaaataaaaa
    23401 gacctgtttt aaactaagga cgaatgactc cgcggtacca ccttaattaa aaacagatct
    23461 gtttttcact ttcaaacttt tattcaaatg tatggacttt ccatgtaatt cgtcaattag
    23521 gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa acacctaacc gctactgcgt
    23581 gaaaagttga cttgtacgaa gtttaccttc cataagatca tttgtcaatg aaaaagaaaa
    23641 tattttttca tttttctgaa aattattttt ataactttca cacacaagat tattttttac
    23701 tagaaagtag cgccctcttg tttttttctt gctactattt cttgatgttc ttgcttgatt
    23761 ctttctaata gctttggctc tttcaacaga tcaagagctg tattggcgag tagttctgct
    23821 gcaatcgcaa tagaagcaag gcctttgtca cttttagcag cagctttgaa ttcttctgtg
    23881 tgtccggcaa tatatgtctc agagattgct accgtaggct ggattgttgg tatgacttgg
    23941 ctgacatttc ctacatcaga agaaccgaga cttgttcttt gttccgtttc tatttcttca
    24001 tctggaacac ctgcttcttt tgcatgctgg aagaataagt catcaaaaga aggagtaacg
    24061 atgacatcat caactgcatt ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc
    24121 gcagcacctc gtacgatatt ttccactttt tcatatactt tatctaatgt ttgccgattt
    24181 cctgcacgta aatagaaacg tccagcagcg taatctggaa tgacgttggc agctactcct
    24241 ccgtctgtaa tgatcccatg gatattgaca tcttttggca attgcaagcg tagagcgttg
    24301 attccattaa acacttggat caatgcttcc aaagcattta ctcctttttc aggtgctgct
    24361 gctgcatggg aagcgacacc gtaaaatttg atgtcgactg gatcattcgc taaagattct
    24421 gtcgttttcc cataacgaaa agctggatgg acgcataacg ctacatcgac atcctcaaaa
    24481 aaaccttctc gaacaaagct gcctttagct gatccgttct cgcccccttc ttcccctggg
    24541 gtgccatata cacggatttc tccaccagtt tcatcaatga attgcttcac tatgctggcg
    24601 gctaatacgg agtaagtacc aaacaaatta tgcccgcatg catgaccaat acctggtaaa
    24661 gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt tattggaaat ataacgtgca
    24721 tcaaaggctg ttcggtgtcc agctacttct ttttctaccc aaaacctttc tttttctaat
    24781 tgtttaatca acgctgctga ggaataaaac tcatagttac ttacttcagg atgttcgtgg
    24841 atatctagag caagtgcttg atattctggt aaacgatatt ggattagatc cttcaattgg
    24901 tttgttgaga ttgttgttgt cataaaaaat tcctcctatg ctagtcattt ttcttatttt
    24961 ctcccgaatg tttcccatgc aggcacgctt gatccttttg atgttttctc gattactttg
    25021 actgtgtctt ctgtttgata agcgtctact attttttgat acgttttatt gttttcatct
    25081 tcttttcttg caacgatgat attcacataa ggacgtgcat ggtcatccac tggttctaag
    25141 aagatggcat cagaagcagg attaaatcca gcatctacag ctacaccgct attgatcact
    25201 gaaatgtcta catctgacaa agcacgtgct gtttgggaag catctaattc agaaatgtct
    25261 agattttttc tattctcggt aatatcagaa acagtgggcg cttgtccttt tgccttatcc
    25321 actttgatca atccagcagt ttgcaataac agaagagctc tgccgccatt tgtaacatca
    25381 ttagggattg ctacttttgc tccgtctttg atatcagcta catccttcaa agtgttagaa
    25441 taaattccca gcggtgcatt gacagtattt ccgatcgata ctaaatcagt tccgtgttct
    25501 ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat tcaaatcaat cgatccatct
    25561 gctaaagcca catttggttg attgtaatct gtaaatttca cgtattccaa attaatgttc
    25621 tcctttttca aacggtcttt cacgttgtcc catacatctg tatcttctcc aataatccct
    25681 agttttacga tagtttcttt tttgtcagtt gtatctgctt ttccttggct acaccctgcc
    25741 aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa taattttttt cattttttct
    25801 cctccacttt caatgtgtaa tttttttaat cagttgatta ctgataaatt gacttaaaaa
    25861 aactaagatt agaatcaata atgtagccac aatcgttaca tctgtttgga atcgattata
    25921 ccctctcgat atagcaagat tccctaatcc gcctgcacct actgctccag ccatggtagt
    25981 cagtccgatc acgttgatga tagtcaacgc cgagacccga atgataccag gcaaaccttc
    26041 taacaaatac acacgtaaaa tgataccgat tggactggtt cccatcgctt ctgccgcttc
    26101 gatcacccca ggatcaactt ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac
    26161 aactccaaaa atcaatggca ctaaagctgc tttcgcacca atcgtcgttc caactaagaa
    26221 acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa gggatggaac gaatgatatt
    26281 gatcagctta tcaagtattt caaatagcag acggttagct agtaatccat tagggcgtgt
    26341 cactactaac gtcacaccta agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa
    26401 agacatatat agtgtttgct tcgtcgcttc gacaaattca tccggaatca aataaacatt
    26461 gggcaaatat gttttaaaga aatcagtcat tttatcttcc tccttcgatc actttgatac
    26521 gactatctaa atcgtttttt tgtaaaatat ttatttttac accagatttg atcaaataat
    26581 ccaatgcttt tttccgctgt ttggtttctc cagctaaagt aacgatcaaa ctccctaacg
    26641 gcgtttcttg taataactct acattgccat ataaaatatt cgctgttact tgatatttgc
    26701 tgtacagatg agcgattaga ggttcgttcg tctgatttcc aatataactt aattccacca
    26761 gccatttatt ggcgatctga tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt
    26821 ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa ttcttcttca ggattgctga
    26881 agatctggat gctgctacct ttttcaacga ttcttccatc ttccataacc gctaccttgt
    26941 tgcagatttc tttgacaact tgcatctcat gagtgatcag gacgattgtt aaacctaact
    27001 ggcgattcaa tttttttaac aatgcaagga tctgaagggt cgttttcgga tcaagtgcac
    27061 tggttgcttc atcacataat aataccttag gttcattcgc caaagctcgt gcgattgcta
    27121 ctcgttgttt ttgtcccccc gacagttgtt taggataagc agatgctttt tcttccaaac
    27181 cgacaagttc cagtagttca ttcacttttt gacgacgttc ctgtttcgat tttccagaat
    27241 actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct
    27301 gaaagatcat accgatggcc tttctttctt ttcgcaattc tttgggagag agctgactca
    27361 gattcgtctg attgacaatg acttctcctg ctgttggttt ctgcagcaaa ttcatcacac
    27421 gaactaacgt acttttccct gcacctgagt aaccgacgat tccataaaca tcgccttttt
    27481 caatcgttaa atctacagaa tctactgcct gtatctcttt ttctttttgt ttgaaacata
    27541 cagaaacatc tcgcaattca atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa
    27601 gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg tgttaccacc tcatgttcaa
    27661 gctgtgttca cacacaactc ctcttcaagt acgatcatac tctagcaaga tatcggtcgc
    27721 agccgtcatc gccttacatc tatgctcggg atgaattgct cgatggccat tttccaatag
    27781 tcgttccttg cttcttctca gctaccgaag ctctctgtca aaggttcttc tatcgtactt
    27841 accatttcaa tgcaaataat attcaaaaaa aaagtccttt tactaacata aactatgtca
    27901 gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa gtttccttcc ctcaagccca
    27961 acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg aacagttccg aatttgccta
    28021 cttatcgaca aattattctc caaggccatt ttcagctttt cttacttgac tcttctcacc
    28081 agccagagcc tctctgcaaa gtttccaaac ttactttcct tttcaacgaa atcgatattt
    28141 tgttgttggg attaatctac atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat
    28201 tttttctata tttatttttc tgttttttta tttaacattt caataacagt tcgaagatct
    28261 tttccttccg caagaagttt caatttttcc ttttcttcga cggtataatt tcggacaaaa
    28321 ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg aagcagctgt tgctcctact
    28381 aaagaatctt ccactgcaaa ggctgaacta gcttctaatc ctgttcgttc caaagctgtc
    28441 agataaggaa tcggtgaagg cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc
    28501 aagtaatcag ctaatttaaa gtgatccaag atatctgttg ctcttttcgt aacagtggtc
    28561 gtagcaatgc ctagtttcat atgacgttct tttaagtctg ccaatatttc ttttgcatat
    28621 ggttttaagt ggatggctcc gcttgtcagc tgttgataaa aatattcttc acgcttcgct
    28681 cggacttcct gtactttttc agggctacct gctgctttca ctaaaatatc aaaactttgt
    28741 ttccagtttt ttccgcccca agcgtcaacg atttcttttg ggatatggat atcaaattgc
    28801 tgaaatcccc aaagccagcc atcacgatac gttttttctg tgtcaactaa caaaccatct
    28861 aaatcaaata ttgcaccttt catctataca ttaatccttt ccaaaacaat acacatagtt
    28921 tagcacgact ggtccaaaaa gaacagaagc aaagaaccag ccttctcgtt tataagagtt
    28981 cttggaatcg acggatatag attttcttca cagctgtcac aagcgccaga taagcgatga
    29041 ctgtagcaaa taaccataac cagtaattgc caggaagtgg catcaagcct aattcagcac
    29101 caaaacttgt aaaaggaagg acagaaccga caataattcc taatgtagtg ataacaaaca
    29161 tggcaaatgt tgcgttgctt tggataaatg gtactttcgg tgtacgcagt gcatggagca
    29221 ctaaggtttg tgtccatagt gattcgacaa accatcctgc atggaataat gcgatgaaaa
    29281 tcactcgctg tgaactatcc aatgtatgga aacttccgcc cactacagca ggacaaataa
    29341 caaaatacat caacaagtaa gtagtaatat caaatatcga acttgttgga ccaaagtaaa
    29401 ccatgaattc cccaatacta gatgcattcc atttttttgg ctcgtccaaa tattctttat
    29461 ccatccgatc ccatggcaag gaaatgcagg aaacatcata gatcagattc aaaaacaaaa
    29521 gttgtaaagg cagcattggt aaaaatggca ggaacgtact tgcaatcagc acggaaaaca
    29581 tattgccaaa attagaactt gctgtagcct tgatatattt catgatattc ccaaaagttt
    29641 ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt
    29701 ctgcagattc tttcgcaata tccacagctg tatctacaga aataccgata tcagccactt
    29761 tcattgcagg agcgtcattg ataccatctc ctaaaaatcc gacagtatga ccatttttac
    29821 gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa ttttacgaat acttcatggt
    29881 cttcagctac ttgtgctaat tcatgatcac tcatttccga gattttctca ccggtaacca
    29941 attcattgat ctctagacct acttgacggc acactgaacg tgtaactaat tcgttgtccc
    30001 cagtcagaac ttttacagct acaccatgtt ctttcagagc tttcaacgct tgtttcgttg
    30061 tttcttttgg tggatcaagg aaagcaagat aaccaatcaa aaccatgtct gactcatctt
    30121 tgactgaaaa ttctccaacg acgcttggat tggtcttttg tgcgacacca atcactcgta
    30181 agccatcttc attaagtgac tgtacttttg caagtacggc ttcttttcgt tcttttgtta
    30241 atggtgtaac agtaccgcca agatcaataa atttagaaat gttcagcatt tcttcaatcg
    30301 caccttttgt gatcatttgt gttttaccag ctggatcagc aaccacaaca ctcatacgac
    30361 gacgttcaaa atcaaaagga atttcatcca ctttttgata attgatctta tctgtatcta
    30421 aagtttctgt tgcttcatca atgatagcca catctaacaa atttttcagc cctgtttgat
    30481 agtagctatt tagatacgca tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca
    30541 tatgatattc aagaatgatt ttatcttgtg tcaatgtccc cgttttatcc gtacataaca
    30601 catcgattgc accaaagttc tggatggcat tcaaattttt gatcactgtt ccttttttgg
    30661 ccatcacact tgctcctttg accaaattcg tcgtaacgat cattggaagc atttcaggtg
    30721 ttagaccaac tgctacagat agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa
    30781 gcccattgat gacgataact gctggagcca tcaatgacat aaatttgatc agtagcatgg
    30841 atgttttatg gatgcctaat tcaaagcttg attgaattgg tttttcagat agcgtttctg
    30901 ctacatgacc aaatagtgtt tcatttccaa cagaaacaat gattccttcc gcacttccac
    30961 tgatgacatt acttcccata aagacaagat tttcgtaact tgtttcgctt tgctgatcgt
    31021 cttcacatac tttcgcatgc ttttctactg gatagctttc ccctgtcaat gctgcttggg
    31081 aaatgaataa atcctttgcg gacaataaac gcatatccgc tggtatcata tcccctgctg
    31141 aaagccgaac aagatctcca cagacgattt cttccatagg aatctctgtg tactttccat
    31201 tacgttttac agctgccgtt actttcacaa gtgatttcag tttttcagcc gcttgattgg
    31261 aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat aaccatagtg aagataatgg
    31321 ctacaccgaa aagatcttta tctccaggag ctgctaatac atattccgtg atgaatgaaa
    31381 taattcctaa tgcgatcaac actaatgtaa atggcgtgat gtatgctttc aatatttcta
    31441 caatgaatgg tgtttttttg ccatatgaaa tcgcattttc accgtaattt tctcttgaac
    31501 gttcgatttg ttcttctgtc aatccatttg ctgatgcatg gaatagttca aataattctt
    31561 gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt atctttcttt ttttccatct
    31621 caatctctcc tttgtgtcca aaagacagct gttctcgctg cttttggtct ttagctattc
    31681 atcggatcga tagagaaaaa actgaaaggg gctgagaaca gatagataat agccctggcc
    31741 tttcctctag atccttgttt ttcgaatgac tgtcatccat ttgttctcat ctcctttact
    31801 aagtaatttt aattcatgac ttccaatcat ttttcgaata gttatttttc caattagacc
    31861 acctcctttt tatttttcac cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat
    31921 actcagcgaa aggtttgaaa aagcatatta aaaaaagctt ctcagatctc atcgttgagt
    31981 tttgacactg tacaacgtaa aaagcagata cctgctttta gctaccttat gaaaagcctt
    32041 atctcgacaa ttcctgttct acccattggc gtctctcgac gtttttgggc agtagcctat
    32101 gtttgtatag gagcctcacc taacaaatct tattcggttt tccttcgtta atataacaac
    32161 taaaaaagag gatgtcaaat caattttcca tgtttttcat acttttttta aaatacacac
    32221 gttatattcc tgtctcacgc ggttttattt gtctcatttt ccacctcccg ctataataga
    32281 aatatagtag gaaacgaggt gctgttatgg aaagaacact tgttattatc aagccagacg
    32341 gcgtgagacg ccatttagtc ggaagcatca tccaacgttt tgaagcaaag ggactggcca
    32401 ttgctgaaat gaagtttgaa actatgactc ctgaattagc aaaagaacat tatcagcact
    32461 tgactgagcg cagttttttt gatgaactga tcgattacat gacttccggt cctgtcgttt
    32521 atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg taagatggtt ggagcaacaa
    32581 aagctgccga tgctgttcca ggtacgatcc gaggagacta cgcgctgccg gggactgaaa
    32641 acatcattca cgcttctgat tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc
    32701 cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg
    32761 ctggtctagt tgctgggttc agcaatggcc agcagttttt tctctaagga cctccgttga
    32821 ttctttccta acatatattc gagcactgtg aagaattctt ggtagtcttt cgtcttttct
    32881 tcccctaaag cagcagaaat caatctttga taataatctg ccatcaatgt ttcatagatc
    32941 actgtacttc tttctttatg tgctaagctc cgtttcatat cttcattcac actacgtgcc
    33001 tttttcaacc gaccattttc tatataaaac gaagtcaggt tcattaaaaa agtaaagaga
    33061 tactgctcat gattgttcaa ctgtgctaat cgtcgcattc tttttttgat tttcaaataa
    33121 ttgttctcaa tgaaccgatc actgaatatc caaggaaaat cagctagaat agatagttca
    33181 aattgaccat agtctggtat ctgcatcaag tattcttcaa tgattcggca ttcataacgt
    33241 aaaaattcaa attctgtctc agatacttct gttttttcga ccatgtgaag attcaaaacc
    33301 aaataataca gatcttctgt tttcttaaaa tgcctacggt tattgctgat ctctttataa
    33361 ggatcaaagc tgtcttcttg tacccgatct agcattcgtc tttttttctt taactttatt
    33421 ggttcatcca gctggtttcg catccattca tattctgcaa gtgtcatgtt cattcgctct
    33481 aaaaatgatc tcaatgttac aaatgacatt tttttcactg aatttgtctc aatcccgact
    33541 agagaagtct taaaacagat tccttccgct aattgggact gtgtcaactt cttgttgact
    33601 cgaagtttct tgatcaattc accatcatac ataatagttc tcctttttaa tttaaatttt
    33661 tccacattca ataaatagat acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg
    33721 tttttttcat aaaaaatacc aaccttcaag cattagaatg ttgtctaact actgaaggtt
    33781 ggtttgattg tttattgatt atttttttgc tgttcaacgt ctgttcttgt tactggatat
    33841 aagttggttg ttgttttatt accatttttt gaaaagacac ttgtcgaatg ttcgcctttt
    33901 tgcttttcga ttgcttcatt acgttccgct tcatttttat agtcatattt tttcggatca
    33961 atcgtctcaa atccgtttgg cacataaaac cgcaagagat tttcctgatt cagcttatct
    34021 gaaagactta acgcagtctt gactttttcc tgatctttgg agatttcctc atcgattcct
    34081 gctgctttcg tatcgatgat ttctcctgtc tgattattgt aagcttttcc tcctaggacg
    34141 gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa ctacttgatc atgctgagaa
    34201 gataataagt ctgtaccaaa atgaagataa tctttatcat cgattcccaa tagatgtagt
    34261 aatgtcggca aaacatcgat ttctccaccg tattcatgat tgactgtccc tttagtataa
    34321 ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat
    34381 gtatctggat ctttaccaag agcgcttgct aaatctttat tatctgtatt cgagatccca
    34441 aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa taccagattt tttcaaatac
    34501 gtaaaaaatt gttccaatga ttgatccaga taatgtgccg ttcgaacata attatctact
    34561 gtgctattgc cagtattcaa agatggaaag tcgaaattct tgtcatccgt ataataaggc
    34621 gtgtggttgg ttacggaaag aaatttcgtg taaaacggct gttgtagatg ctcaagatat
    34681 tgggcagatt ctctgaataa atctttatct aatatcccat aacctaacgt ttcgtcggat
    34741 tcatcaaaat aagatcgatc aaagaaattg tcataaccta agtttttata tacatgatct
    34801 ctattccaaa aactagctac attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt
    34861 ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc caagttctgt gaataatgag
    34921 ccttgaggta aaccaaatgt gccagtttcc aacatatttt cagcatcact tgtctttccc
    34981 tgtcctactt catgaaagaa attgtcaaag ctgatcgttg cttgattctg gaaaatacta
    35041 tttaaaaacg gtgtgacttc ttgtccgtct accttcatat taatcaaaaa ctgctggaaa
    35101 ctttctaaat ggagaacgat cacatttttc cctttagcga tcccaaatgt ttcgggattt
    35161 ggctctgcat aatgtttctt tgtataatct aaaacttcat caatcccatt actgcttgca
    35221 tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt cataaatagt atacgcatcg
    35281 attcctagat atttgacaat atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg
    35341 ttggcttcgc tcagcgctag atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg
    35401 atagccatcg gtttgccgac agggttgctt ttgataggta ttttcttgac cttcaaccaa
    35461 accagtagcc caatgaacac agcgatatcc agccagtaga tgacatcatg gggcttcatc
    35521 aaagaaaagg agcttcctga caatccttgc gacactttcg aaaagcctaa tacagatttg
    35581 actgtaatga aatctgtgaa ttcccgataa aaaataatat tcaagtacaa caaaagcgta
    35641 tttaaaatat caatgatcaa aagaatagct aacgctggct ttggcttttt aatatacaaa
    35701 gaaaggccaa aaaacaataa ggtagtcgca attgggttga tccacaagat aaagtactgg
    35761 atcgtacctt ccacacctaa tgaaaaatct gcatagtagg caatgatcgt cttcagccag
    35821 aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt ggcacaattt tttaagttgt
    35881 gtcacgtata tcacttctct ctattcacat tttttcttat tttgctcctg aaaaatgaaa
    35941 atttcctaaa aaagttgtta tcttaagatt tttttattct ttctaaagat tattccgtcc
    36001 acttttcaca gcataaaacc gctgcgatgt aagcgattaa actaatgaag agataaaagt
    36061 cttgtggtga ccgcttcact ccacggtatt caaaagccag tttctcggta ttcgtacaaa
    36121 aaaagaggct gtgacaaaat tcttgtcgca gctccttatt tcttgatgca ggacagcttt
    36181 ttcacaactt cttccttaaa cggtgttctc ctgccaactc ctcggccaca agtcacaata
    36241 tttataaaac atgagaagct gttttttgaa attgttcctt gtggctcaga gttaaacggc
    36301 aggctcaaga cctcttgata cacggtgttc aatcagctga ccctcggtat tagattatgt
    36361 caggacgcta attttacaac ctcttggtaa acggtgttca aaagcttgca tctgcggtaa
    36421 taagcccaac caaacgaaaa atataaggag ctattttttg aaattgctcc ttgtggctca
    36481 gagttaaacg gcaggctcaa gacctcttga ta
//
LOCUS       DACJGB010000021        35882 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.021, whole genome shotgun sequence.
ACCESSION   DACJGB010000021 DACJGB010000000
VERSION     DACJGB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.021"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..1152
                     /locus_tag="IYI34_001123"
     CDS             <1..1152
                     /locus_tag="IYI34_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559002.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IYI34_001124"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IYI34_001124"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAR0559003.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IYI34_001125"
     CDS             2728..3003
                     /locus_tag="IYI34_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAR0559004.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IYI34_001126"
     CDS             3009..3776
                     /locus_tag="IYI34_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAR0559005.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IYI34_001127"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IYI34_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAR0559006.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IYI34_001128"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IYI34_001128"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAR0559007.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IYI34_001129"
     CDS             7920..8222
                     /locus_tag="IYI34_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAR0559008.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IYI34_001130"
     CDS             8225..9388
                     /locus_tag="IYI34_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAR0559009.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IYI34_001131"
     CDS             9414..12842
                     /locus_tag="IYI34_001131"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAR0559010.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IYI34_001132"
     CDS             12863..13987
                     /locus_tag="IYI34_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559011.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IYI34_001133"
     CDS             13977..14414
                     /locus_tag="IYI34_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAR0559012.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IYI34_001134"
     CDS             14398..14736
                     /locus_tag="IYI34_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAR0559013.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IYI34_001135"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IYI34_001135"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAR0559014.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IYI34_001136"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IYI34_001136"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAR0559015.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IYI34_001137"
     CDS             15893..16936
                     /locus_tag="IYI34_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAR0559016.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="IYI34_001138"
     CDS             17095..17823
                     /locus_tag="IYI34_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAR0559017.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="IYI34_001139"
     CDS             17939..18184
                     /locus_tag="IYI34_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAR0559018.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="IYI34_001140"
     CDS             18181..18399
                     /locus_tag="IYI34_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAR0559019.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="IYI34_001141"
     CDS             18535..19074
                     /locus_tag="IYI34_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAR0559020.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="IYI34_001142"
     CDS             19067..21340
                     /locus_tag="IYI34_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAR0559021.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="IYI34_001143"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="IYI34_001143"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAR0559022.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="IYI34_001144"
     CDS             complement(22513..22767)
                     /locus_tag="IYI34_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAR0559023.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="IYI34_001145"
     CDS             complement(23167..23436)
                     /locus_tag="IYI34_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559024.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="IYI34_001146"
     CDS             complement(23906..24361)
                     /locus_tag="IYI34_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAR0559025.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="IYI34_001147"
     CDS             complement(24499..26427)
                     /locus_tag="IYI34_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAR0559026.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="IYI34_001148"
     CDS             complement(26624..27457)
                     /locus_tag="IYI34_001148"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAR0559027.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="IYI34_001149"
     CDS             complement(27649..28353)
                     /locus_tag="IYI34_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR0559028.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="IYI34_001150"
     CDS             complement(28376..29002)
                     /locus_tag="IYI34_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR0559029.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="IYI34_001151"
     CDS             29328..31703
                     /locus_tag="IYI34_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAR0559030.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="IYI34_001152"
     CDS             complement(31727..32218)
                     /locus_tag="IYI34_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAR0559031.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="IYI34_001153"
     CDS             32405..33325
                     /locus_tag="IYI34_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR0559032.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="IYI34_001154"
     CDS             33479..33823
                     /locus_tag="IYI34_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAR0559033.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="IYI34_001155"
     CDS             complement(34013..34513)
                     /locus_tag="IYI34_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0559034.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="IYI34_001156"
     tRNA            35041..35124
                     /locus_tag="IYI34_001156"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
     gene            complement(35448..>35882)
                     /locus_tag="IYI34_001157"
     CDS             complement(35448..>35882)
                     /locus_tag="IYI34_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAR0559035.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11868 a         6262 c         7405 g        10347 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaata atgaatctta gtaccaaccg
    35461 tttgcttccc agaatgcttt cgccgcttcc caagaaccat aacgtgaagt aacatattgt
    35521 tctgctactc tttcttggtt tgcagcagag tagtcaccat ttaagtaaga agaatctaat
    35581 tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag aaccgcttga ttctttttgt
    35641 gcgatccatt cttttgcaga gcttgtactt gctggagtag cagcagtttc tgttacagca
    35701 ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt ccgtttgaac cgtttcttgt
    35761 acaacaggtt gttcagcttg aacaggttca gcttggactg gtgcttcttg aacggctgct
    35821 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag
    35881 ta
//
LOCUS       DACJGB010000022        34961 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.022, whole genome shotgun sequence.
ACCESSION   DACJGB010000022 DACJGB010000000
VERSION     DACJGB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.022"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            155..1861
                     /locus_tag="IYI34_001158"
     CDS             155..1861
                     /locus_tag="IYI34_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAR0559036.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="IYI34_001159"
     CDS             complement(2019..2921)
                     /locus_tag="IYI34_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR0559037.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3622)
                     /locus_tag="IYI34_001160"
     CDS             complement(2918..3622)
                     /locus_tag="IYI34_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAR0559038.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="IYI34_001161"
     CDS             complement(3653..4294)
                     /locus_tag="IYI34_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR0559039.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4646)
                     /locus_tag="IYI34_001162"
     CDS             complement(4332..4646)
                     /locus_tag="IYI34_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559040.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4634..5263)
                     /locus_tag="IYI34_001163"
     CDS             complement(4634..5263)
                     /locus_tag="IYI34_001163"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAR0559041.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="IYI34_001164"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="IYI34_001164"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAR0559042.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="IYI34_001165"
     CDS             complement(5983..6915)
                     /locus_tag="IYI34_001165"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAR0559043.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="IYI34_001166"
     CDS             complement(6912..7688)
                     /locus_tag="IYI34_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAR0559044.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="IYI34_001167"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="IYI34_001167"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAR0559045.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="IYI34_001168"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="IYI34_001168"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAR0559046.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="IYI34_001169"
     CDS             complement(12013..13293)
                     /locus_tag="IYI34_001169"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAR0559047.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="IYI34_001170"
     CDS             complement(13312..14238)
                     /locus_tag="IYI34_001170"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAR0559048.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="IYI34_001171"
     CDS             complement(14251..15534)
                     /locus_tag="IYI34_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAR0559049.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="IYI34_001172"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="IYI34_001172"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAR0559050.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="IYI34_001173"
     CDS             complement(16322..17233)
                     /locus_tag="IYI34_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAR0559051.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="IYI34_001174"
     CDS             complement(17230..17694)
                     /locus_tag="IYI34_001174"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAR0559052.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="IYI34_001175"
     CDS             complement(17699..18397)
                     /locus_tag="IYI34_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR0559053.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="IYI34_001176"
     CDS             complement(18384..18770)
                     /locus_tag="IYI34_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559054.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="IYI34_001177"
     CDS             complement(18917..20587)
                     /locus_tag="IYI34_001177"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAR0559055.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="IYI34_001178"
     CDS             20770..21003
                     /locus_tag="IYI34_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAR0559056.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="IYI34_001179"
     CDS             complement(21102..21533)
                     /locus_tag="IYI34_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAR0559057.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="IYI34_001180"
     CDS             21612..22025
                     /locus_tag="IYI34_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAR0559058.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22063..23211)
                     /locus_tag="IYI34_001181"
     CDS             complement(22063..23211)
                     /locus_tag="IYI34_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAR0559059.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23333..23929)
                     /locus_tag="IYI34_001182"
     CDS             complement(23333..23929)
                     /locus_tag="IYI34_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR0559060.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="IYI34_001183"
     CDS             complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="IYI34_001183"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAR0559061.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27159..27590
                     /locus_tag="IYI34_001184"
     CDS             27159..27590
                     /locus_tag="IYI34_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0559062.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27635..28984)
                     /locus_tag="IYI34_001185"
     CDS             complement(27635..28984)
                     /locus_tag="IYI34_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAR0559063.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..30217)
                     /locus_tag="IYI34_001186"
     CDS             complement(29054..30217)
                     /locus_tag="IYI34_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559064.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30346..30669
                     /locus_tag="IYI34_001187"
     CDS             30346..30669
                     /locus_tag="IYI34_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559065.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30852..32201)
                     /locus_tag="IYI34_001188"
     CDS             complement(30852..32201)
                     /locus_tag="IYI34_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0559066.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32270..33223)
                     /locus_tag="IYI34_001189"
     CDS             complement(32270..33223)
                     /locus_tag="IYI34_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAR0559067.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33325..34515)
                     /locus_tag="IYI34_001190"
     CDS             complement(33325..34515)
                     /locus_tag="IYI34_001190"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAR0559068.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7642 c         6120 g        11248 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttaaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
    22141 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
    22201 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
    22261 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
    22321 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
    22381 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
    22441 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
    22501 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
    22561 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
    22621 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
    22681 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
    22741 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
    22801 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
    22861 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
    22921 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
    22981 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
    23041 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
    23101 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
    23161 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
    23221 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
    23281 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
    23341 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
    23401 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
    23461 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
    23521 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
    23581 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
    23641 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
    23701 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
    23761 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
    23821 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
    23881 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
    23941 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
    24001 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
    24061 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
    24121 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
    24181 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
    24241 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
    24301 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
    24361 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
    24421 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
    24481 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
    24541 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
    24601 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
    24661 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
    24721 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
    24781 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
    24841 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
    24901 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
    24961 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
    25021 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
    25081 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
    25141 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
    25201 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
    25261 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
    25321 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
    25381 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
    25441 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
    25501 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
    25561 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
    25621 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
    25681 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
    25741 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
    25801 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
    25861 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
    25921 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
    25981 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
    26041 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
    26101 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
    26161 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
    26221 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
    26281 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
    26341 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
    26401 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
    26461 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
    26521 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
    26581 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
    26641 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
    26701 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
    26761 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
    26821 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
    26881 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
    26941 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
    27001 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
    27061 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
    27121 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
    27181 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaataata tcttaaataa atgccaaacg gtatgagtaa taaaacatta aaagctggtt
    29881 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
    29941 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
    30001 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
    30061 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
    30121 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
    30181 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
    30241 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
    30301 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
    30361 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
    30421 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
    30481 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
    30541 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
    30601 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
    30661 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
    30721 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
    30781 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
    30841 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
    30901 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
    30961 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
    31021 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
    31081 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
    31141 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
    31201 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata agggagacgc
    31261 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
    31321 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
    31381 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
    31441 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
    31501 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
    31561 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
    31621 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
    31681 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
    31741 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
    31801 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
    31861 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
    31921 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
    31981 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    32041 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    32101 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    32161 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    32221 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    32281 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    32341 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    32401 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    32461 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    32521 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    32581 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    32641 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    32701 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    32761 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    32821 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    32881 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    32941 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    33001 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    33061 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    33121 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    33181 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    33241 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    33301 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    33361 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    33421 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    33481 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    33541 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    33601 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    33661 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    33721 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    33781 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    33841 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    33901 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    33961 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    34021 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    34081 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    34141 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    34201 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    34261 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    34321 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    34381 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    34441 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    34501 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    34561 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    34621 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    34681 atatagtatg aacaattacc ttttagataa tgatctattt tactctacct gggcagataa
    34741 atacaacaca accattgata gaagcccttt cgttaaaatc aattgtattc tgaatattta
    34801 atttatttat tgcatagacc aataaaaata cataaaattt ccatcttcgt ttatcagcaa
    34861 tttttaattt tactctctga atatttctta aaagttttac ttgttgaatt cgaaaaaagg
    34921 actgtgacaa aagtcttgtc acagtcctat tcttccgaat a
//
LOCUS       DACJGB010000023        34836 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.023, whole genome shotgun sequence.
ACCESSION   DACJGB010000023 DACJGB010000000
VERSION     DACJGB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.023"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IYI34_001191"
     CDS             complement(<1..2322)
                     /gene="esp"
                     /locus_tag="IYI34_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAR0559069.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFT"
     gene            complement(2939..3244)
                     /locus_tag="IYI34_001192"
     CDS             complement(2939..3244)
                     /locus_tag="IYI34_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559070.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3389..4594)
                     /locus_tag="IYI34_001193"
     CDS             complement(3389..4594)
                     /locus_tag="IYI34_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAR0559071.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5288..5674
                     /locus_tag="IYI34_001194"
     CDS             5288..5674
                     /locus_tag="IYI34_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAR0559072.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5772..5963
                     /locus_tag="IYI34_001195"
     CDS             5772..5963
                     /locus_tag="IYI34_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559073.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6477..6809
                     /locus_tag="IYI34_001196"
     CDS             6477..6809
                     /locus_tag="IYI34_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0559074.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7106..7261
                     /locus_tag="IYI34_001197"
     CDS             7106..7261
                     /locus_tag="IYI34_001197"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAR0559075.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7537..8877
                     /locus_tag="IYI34_001198"
     CDS             7537..8877
                     /locus_tag="IYI34_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR0559076.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(9118..9525)
                     /locus_tag="IYI34_001199"
     CDS             complement(9118..9525)
                     /locus_tag="IYI34_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAR0559077.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(9714..10541)
                     /locus_tag="IYI34_001200"
     CDS             complement(9714..10541)
                     /locus_tag="IYI34_001200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAR0559078.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            10850..11068
                     /locus_tag="IYI34_001201"
     CDS             10850..11068
                     /locus_tag="IYI34_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559079.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            11089..11724
                     /locus_tag="IYI34_001202"
     CDS             11089..11724
                     /locus_tag="IYI34_001202"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR0559080.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11917..12156)
                     /locus_tag="IYI34_001203"
     CDS             complement(11917..12156)
                     /locus_tag="IYI34_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559081.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12180..13703)
                     /locus_tag="IYI34_001204"
     CDS             complement(12180..13703)
                     /locus_tag="IYI34_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAR0559082.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(13721..13843)
                     /locus_tag="IYI34_001205"
     CDS             complement(13721..13843)
                     /locus_tag="IYI34_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAR0559083.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(13873..14856)
                     /locus_tag="IYI34_001206"
     CDS             complement(13873..14856)
                     /locus_tag="IYI34_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAR0559084.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14880..15029)
                     /gene="rpmG"
                     /locus_tag="IYI34_001207"
     CDS             complement(14880..15029)
                     /gene="rpmG"
                     /locus_tag="IYI34_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAR0559085.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15050..15427)
                     /locus_tag="IYI34_001208"
     CDS             complement(15050..15427)
                     /locus_tag="IYI34_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559086.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15459..15638)
                     /gene="rpmF"
                     /locus_tag="IYI34_001209"
     CDS             complement(15459..15638)
                     /gene="rpmF"
                     /locus_tag="IYI34_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAR0559087.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15653..15922)
                     /gene="rpsN"
                     /locus_tag="IYI34_001210"
     CDS             complement(15653..15922)
                     /gene="rpsN"
                     /locus_tag="IYI34_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAR0559088.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16445..18217)
                     /locus_tag="IYI34_001211"
     CDS             complement(16445..18217)
                     /locus_tag="IYI34_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559089.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18171..19925)
                     /locus_tag="IYI34_001212"
     CDS             complement(18171..19925)
                     /locus_tag="IYI34_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559090.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20035..20604)
                     /locus_tag="IYI34_001213"
     CDS             complement(20035..20604)
                     /locus_tag="IYI34_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAR0559091.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20622..22046)
                     /locus_tag="IYI34_001214"
     CDS             complement(20622..22046)
                     /locus_tag="IYI34_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR0559092.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22048..22716)
                     /locus_tag="IYI34_001215"
     CDS             complement(22048..22716)
                     /locus_tag="IYI34_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAR0559093.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22847..23431)
                     /locus_tag="IYI34_001216"
     CDS             complement(22847..23431)
                     /locus_tag="IYI34_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0559094.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23766..23996)
                     /locus_tag="IYI34_001217"
     CDS             complement(23766..23996)
                     /locus_tag="IYI34_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAR0559095.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(24011..24961)
                     /locus_tag="IYI34_001218"
     CDS             complement(24011..24961)
                     /locus_tag="IYI34_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAR0559096.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24958..25800)
                     /locus_tag="IYI34_001219"
     CDS             complement(24958..25800)
                     /locus_tag="IYI34_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAR0559097.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(25933..26658)
                     /locus_tag="IYI34_001220"
     CDS             complement(25933..26658)
                     /locus_tag="IYI34_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAR0559098.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(27119..27433)
                     /locus_tag="IYI34_001221"
     CDS             complement(27119..27433)
                     /locus_tag="IYI34_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAR0559099.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(27449..29044)
                     /locus_tag="IYI34_001222"
     CDS             complement(27449..29044)
                     /locus_tag="IYI34_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAR0559100.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(29067..29957)
                     /gene="iolE"
                     /locus_tag="IYI34_001223"
     CDS             complement(29067..29957)
                     /gene="iolE"
                     /locus_tag="IYI34_001223"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAR0559101.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(29974..30999)
                     /gene="iolG"
                     /locus_tag="IYI34_001224"
     CDS             complement(29974..30999)
                     /gene="iolG"
                     /locus_tag="IYI34_001224"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAR0559102.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(31023..32030)
                     /locus_tag="IYI34_001225"
     CDS             complement(31023..32030)
                     /locus_tag="IYI34_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR0559103.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(32143..33303)
                     /locus_tag="IYI34_001226"
     CDS             complement(32143..33303)
                     /locus_tag="IYI34_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAR0559104.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(33379..34833)
                     /locus_tag="IYI34_001227"
     CDS             complement(33379..34833)
                     /locus_tag="IYI34_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAR0559105.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT        10898 a         6941 c         5335 g        11662 t
ORIGIN      
        1 ggtgaaggtc actttgtggt agcctgcagg aatgttttct cctggatttt cataaatatc
       61 tttacttaca gttactgcta aatcggtcgt gcctacagaa ccatcttggt aaacaatttt
      121 aataggaatt gttccagtaa tcggctccga ttctcctaca cctttagctg gagtgaatgt
      181 aacaatacca gtatttgcat caacacttgc attaccgaag tccttattat agacatctaa
      241 aattgaaaag gcatctttag ctaaaggaac atctggttta tcaaatgtag gttttccact
      301 ttgaactgtc gtttctggag ttgttgttat aggtacgtat gttgcatcat tttccatagc
      361 ttgagaagct tttacagcaa actctacatc cacgtcgaaa gttcgatttc cgatcttaac
      421 agttacaata cctttcgtac tatcagtaac gttagaagta tcaggctctg ttttccatga
      481 atactctgca tcttcaggta agtttttatt attccgaatt gattctttag catctggttt
      541 aaatccaact accacggttt gtttatcaac cgcttttggt gattccttaa taacggttga
      601 accttcttct ggtttatcaa aacctggaga aacgatttgg atttttaatt catctttcgc
      661 gattaattta cttgaatcta cacccgtaaa ttcaaattct acagtatctg aaattggagc
      721 cccatctttt tcatttggag cgatagtttt ttctgctaat acagtacctt ctttatcaac
      781 aagttgggct ttgtgacctg ttcccgctaa ctcgtggatg aatactttcc ccttaactgt
      841 tgtgtcaaca tcatatactt cttctaattc aggtttcccg atatatggat tcgtgtctcc
      901 gctctcttct ttatcgtcag ggtttccatc accatcaata tcttgcaaat aataataacc
      961 tgttccataa gtgttctgga ttaatttttt attcgtatcc gacaaccaag aatcaaagat
     1021 gaaatcttca ccaaatggtt tattttcttc aacttgtgct gttgctttca tatcttctaa
     1081 gatttcatct aatggcttag tcaattgtaa aacatatcga ataacgactg gacgtggact
     1141 tgcattagca aaatcaataa gtttggtaag atcattatca gtaattgaac ctacaccata
     1201 attttcatga tctttgcgcc gttcaattgc taaatcagcc acttttttcc ctgattcaaa
     1261 agaaacatca tatgttactt tgtcagcctg aaccatgtgc atctgtatac ccgctacata
     1321 tggaactaat cgttcatcaa ttttgtaatt agcataccaa ggcgtgccct ttgcggaagc
     1381 tgaatatgag aaattataat ttttcgttaa cttatgatct aagattatag ctccattagc
     1441 acctgtggat cctgaaccat cttttgtttg agtattaggt tcctcatatt ttgcacttac
     1501 tgatccatgt ttaaactggt caggattatt ttcagataca tctaagctat tatagaaatc
     1561 atctgaatct gttaaaaagt agcccgtact atcaatacct ggtacaattt tatcatcgtc
     1621 ttgatgactt accaagtaga ttcgatacat taaacgatcg gtcttgagat tgtctatgcc
     1681 tatctcttta aaaatttcat ctaagctctt ttcaaacgta attaccccat taggagcagt
     1741 ttgtgtatca gttgtttcag ccccagcaaa taatccatca cttgcacgaa tataattgac
     1801 cttccaaata tttgtaggtc tacccagcga atcattaatt cttacaaagg tccgtctaga
     1861 agatccaatt cctttctttg gatcgacctg aattttttct acatatttag ctaatcgttc
     1921 atctaattga agacggattt cccagttttt agttgatgct gcaatattat gttttccgta
     1981 aacttcaaat ccaattgtat tgccttttag tgtgtctcca gttagttctt gaggactaaa
     2041 ttgtaaatcc gaaaagttat aatttttcgt ataaccagga ttctcctgaa aagtattccc
     2101 cgaagctact ttgtcaccta cttgtacgga gcttatcgct gtttcaagtg gggtgcttgg
     2161 agaaatttca tcttgtgctt gaactagttc agttgttccc aagacaagac caacaccaac
     2221 caaaacagat gcaaccccaa cacttaattt tttgatactg tacttcaata ctcgtttctt
     2281 tgttttttct agaaatactc tcttattatt cttgctaacc attattcaaa tcaactccct
     2341 taatagttat tttcttagta agaataaaag ataaatctga tacgtattat tcacagaata
     2401 ggaatcttac taaacttatg ttaactagtt tacttaaaga atttcatgca cattacaaca
     2461 aaaaaagtac atttcttcat gtttttttcg gatatttgtg ctttttaacc attcagcagc
     2521 tgaaaacata taaaaagata tgtatgtatt ctttctgaaa ttaagttcat ttctatgcga
     2581 tgaagcatgt tttcaaaata ttactctttc ttagattaaa cttcagagta ttcaaatagc
     2641 ggaataagga gccttatccc gcattactga atgagactgt gacataagta aaaaggtgac
     2701 ttcaaattcc ttcttcggaa tttgaaatca ccttctttat aattataaag tatcggtttc
     2761 acagcattcc tgaatgattc ctagaatctt aaacgaatga ttggagttat caacattttt
     2821 tcactcgttt ttagcgaaac aaaaaatgac atttgtcaca gcctcattaa attattgatt
     2881 ctttttcttt ataattgaat tgtccatatt taaagtgtct agtactctac atgaataatt
     2941 aattgctttc actcaaatac ttataatcta ttaacttgta gttgtttaac ttttctgaaa
     3001 aagttagacc cacgtaataa tcttcccagc tttctctttc aattaactga tccaattttt
     3061 gataatccat aaattcatca tgtattaaaa agaataaata acttgatatt ttagcatcta
     3121 gcttaatcag attaaataat gtttgcttaa aattgctacc attaatatga ctgatagatt
     3181 ctctagcttc ttcacataaa ctttttactt tattaattac tactttgtca tattcattta
     3241 acacactatt attaccatta cttagacgct cagattctac gaaacaactc aaatcagaaa
     3301 tttcccaaaa aattttttgt taaaatccaa tttcctcaac tcctccaaac tacgtaaatg
     3361 atatagtctc ataataaaat aaaacctctc agctaatgtt gttgaaatta tccactaaac
     3421 ctaattgata ttgcttagga gatacacctg cgtacttttt gaatactctc gaaaagtgtg
     3481 aagaatctga aaatcctaaa cctaaagaaa cttcactgat agtatggttt gatttcaaca
     3541 ttattttagc cgtagaaatt tttcgcttag agatataatt ataaatcgta atatgcattt
     3601 cattcttaaa gctttctctt aatttagttt cacttacaga aaaataatta gctatttcct
     3661 ttacagtaat tcttttatag atatttaagc cgatgaattg aacgacagaa gaaattaagg
     3721 gagaaagatg ctctatctta gtttttccga tttccttagt ataataaaca atcccagaat
     3781 ccctcacttt taagacctca ggtaacgata cagctaattc gttttcttgt atcaaagcat
     3841 ctctactttg atacgcttct attatatcca ttcctaatgt tatagacgtt tgtgctaact
     3901 tttcaaaaac aatgatagaa taattttttt cagaccgaat cgtatctcca ctaacacttg
     3961 gaattattcc attactcaga ttaaaaacca tgttttctaa atttcgaata tctccatttt
     4021 taacatattc cagtatttta ttttcataaa gaaacagata tatttctgga tcgtaatttt
     4081 gtgataacag attatctatt ttgatttgct cgatagtttt acttagattt cctgtatatt
     4141 caattaacgg ttcatggaat agatcttcta tttttccagt aaaaaaataa tgaactaaaa
     4201 tcagaatatc tcgaatgtct cctaaagaat acaaagtcaa attttgcaat gaattataga
     4261 gtcgttctct atcagccatt gcaacattca aattgttcat ttttagttta aaaaaatctt
     4321 tatcaatatt atttgctcta aatggaccaa ataagtaata tctggtttta aacatgtata
     4381 gaagaaacat ctcattaaaa tcaccagtaa agaaatggaa atctttagtt tttttaatat
     4441 tatcagttag taacttttta taatcataaa aaaaataagt agttctatct gagcgatact
     4501 cttttataat actgaattcg gaattaaaaa ccacgatagg taactttaaa caaacatgaa
     4561 gagactttaa taagacttct ttatctgaaa acacttttgt ttctctcttt cctttctaca
     4621 tcattaaata gtattttata cgttatctaa aatactattc ctctgtcaaa ataagctaac
     4681 ttacttcatt ataaaatatc cacttatcgc tttcatgtat attccaccgt aaaaagtaca
     4741 ttttatcacc gagaactcat catttttcat catgatgaat actatttaca tatatctagt
     4801 tcaaagacaa taattattat gatccgttat tatctaagca ctatacagat aataggaatt
     4861 tttcgtttct aaatactcca atttgggaat ccaataaaaa ttttcgttca aatacgtgtc
     4921 tattcgaact cttttctagt tgttaattat gaagggggaa gaaataaaaa atacaatcta
     4981 tacacgaata agaatattat ccaaaaggag gttttagaaa agatttatta tacgtaaaaa
     5041 gctcaagaca ttttcttagc cataaggaca tgtttgatat ttaagaagat atatgaattt
     5101 tcttttctaa gtataaagat aactatccat tatcaactaa aaaaacattt ttgaaaataa
     5161 acatcagcag tcctatcaat gcatgtgagc ttatagaagt tattcgcagc gcagcataat
     5221 tgcattagct tcttttcgtg ttttttgcct cttttccctt taaatcttaa tggaggaggt
     5281 gtgcaatatg tcagataacg acgaacgcgt aaatgcccag ttgattcgat tctttgaaaa
     5341 ggtcattttg aacaccgcta atacttatta taaaaaacaa gaagaaattc aaaaacatga
     5401 acaacttgat caactcccac catatttgtt tactaaagat actataaaat ttaaacaacc
     5461 gattaatgtt ttaaatgtga ctctccttgt tgaagatata aaattagcaa aaatccttcc
     5521 tttgttgaaa gaacctgaac aaatttttct tatcgaaaaa tttatcttta ataaaactga
     5581 taaagaaatt ggaaaggttc tgggtattac aagacagggt gcgacaaatt taaaacatcg
     5641 actttacaaa aaaattcaaa aaatgctggt ttaatatctg cataataact aattttgtat
     5701 gcttctttgc tcgtcctcct ccttccccaa aaaataaata gattataagt tcatgggaag
     5761 aaggtaaatt tatgaatact ttaaaagaag atttcatttt agcaaagaat ggaaatgaag
     5821 aagcagttgt ggccattctc aaaagattta gcgctttggt ccataagcaa tcctggagaa
     5881 ctggaaaata tgatcaagat tgttatcaag aatgtatgct tgcaatatat ctagctatct
     5941 cgaaattcga aattaaagaa taactcaact aagggagtct gtgacataag taaaaagata
     6001 gtttcaaatt tcgaacaata aatttgaagc catcttcttt ataattataa agtatcgatt
     6061 ttacagcgtt ctataatgat tcatagaatc taaaacgaat gattttaatt attaacgttt
     6121 tttaattcgt ttttagctta aaaataactt ttgtcacagt ctcactccta aaattacttt
     6181 atttacgtac gaagtgggca ggaggtcgaa tccagaactg ggccatggta cttcaacgag
     6241 gacttctcct ctagaattga aaagcatgcg atttaccttc cgtaacgacg gtagatttat
     6301 tcaactatgt gtccaatcaa aatttgtctg atttcaaaaa tagtcaagag aattttggtt
     6361 gttaaaaact cttatctatc aacttccaca gtttagttga caaaccctga tactaagttg
     6421 ggctatgata cacttttctt gactatcagt tacgttaccg gaaggaatga actctattga
     6481 aaaaaacata tgatcgtaaa tttaaactaa agatttcaca ggatatcctt gagaaaaaag
     6541 taactaccaa gaaaattgct gaagaataca atatctctcg tccaacaatt tcccgatggg
     6601 tttcagaata tcgtcgatac gggaaaaatg cttttgctgg acaaggaaaa agattacctg
     6661 ataaagcaga tttctacatt cttgaacagg aaaataaacg actaaaagag gaagacgagt
     6721 gttctacgaa cacaaagatc gatacggatc tccagggatc actcaaaaac tttacgatga
     6781 aggaatcgaa atcaataaac gtgttgtagc tgttcttatg cgtgaaatga atttatgcac
     6841 aagaggattt catcgacgca aatcttctca tggtagagaa aaaacaatca aagaaatggt
     6901 aaaaaaaatg ttgaatcgat aatttgaaca gaaccaaatt gatgctgttt gggtgacgga
     6961 tattacttac attccatgta atgacggtcg cctctatttg tccacctata ttgacttagc
     7021 aactcgtatt ctacgttact atatggtgga ttcacacatg aaaaaagaga ttgtcgtccg
     7081 tccattacaa atttattaag gagaattgcc tacggtaatt cattccgata gaggtagcca
     7141 gcatcggtct tttatttatc aagagctgtt gaccgaccac cacatcacac attcaatgag
     7201 ccaaactggg actcctgttg acaatgcagt aattgaagcg tttcatcgat caatcaaatg
     7261 agaattaatc gactccaaca aatataaaag taaaatagag atgaaggtgt taatccaaga
     7321 ttatttagaa gactactacc caaacaaaag aatccataca aaatttatga tgactccttg
     7381 acaatttgaa gcgaagaaaa taagggatct ataatatcta gtgttggtga cgccttctgt
     7441 tgccaatact ttttcttttt tctaataaac gcaaacagac accttgaaat cctttagaat
     7501 aaagtcgacg aaaaccattc aaaggagtgt ttcaagatgt cctattcaga gtttaccaaa
     7561 gagttactag atattctaga cctaaatctt acctttcatg aagatgcctt tcgcaaagag
     7621 cgcattaatg acgaaacatg ttttgttttt gatggcacat tgacctatca accagaggaa
     7681 tgcttccatt gccactatca aaataagcaa acgatcatta aatggggctg gaaaaaagta
     7741 tccatcttat tgaatgatgt cagtaactac aagaccattg tgcggattaa caaacagcgc
     7801 ttcaaatgta aacactgtgg aaagactttt ttagcagaag attcagttag cgatcgtcgc
     7861 tgctccattg cccgcagagt caaacaagcc attcttgagc tattaagtga gcctatttct
     7921 atgtctttga ttgcacgtat gaagcacatt tctccgacca ctgtcattcg aatacttcgc
     7981 agccttcggc ctaaaaccgt ttctttgaac cagccgttac cagaagtggt gtgtttcgat
     8041 gaattcaaat cggtgaaaaa cgtctcggga gccatgagct ttgtcatgat ggacggcaag
     8101 acccatcaac tgattgatat tgtggaaaat cggcaactca atcctttacg cgactatttc
     8161 ttgcgctacc ctagaaaagt ccgtgaacaa gtacgtctag tggtttcaga tttctataca
     8221 ccctatcgca cattggtgaa agagtgtttt ccgaatgccc gaatcgtcgc cgatcgcttt
     8281 catattagcc aacacatcgg tcgggccttt accaaccacc gaattcaagt aatgaagacc
     8341 tttaaaaaag gggatcgtcg gtcgaagcac ttaaagaaat actggagact acttcaaaag
     8401 aatgcgtggg aattgaatgg tcagcatcgc tactggcgtc cgtcatttcg agatcatctg
     8461 acagaagcgg agatagtcga tcggctatta tattatgatg actccttaaa gcgaggctac
     8521 gaagtctacc aggtttttct ttccgctatc agaagacaag atgttcctga attcgtcgcg
     8581 ctcctaaaag aagactacaa ggagttgcca gagcactatc aaccagtgtt cacgaccttc
     8641 aagaaatatc gaacagaaat caaacgagcg ttgcgagttc cctattccaa cggccctata
     8701 gaatgtttga acaaccatat caaagtacta aaacgaattg cctatggctt tagaaacttt
     8761 caaaactacc gagaacgaat ctttttatat cgaggaaaat atttcaagaa aactaagaac
     8821 actacccaac tgactaaagc cagaacaaca acacggctag acaagatagc tgtctagccg
     8881 tgtgagtatc tcgattattc tattgaattt cttcaccaac actcattgac gaagagccaa
     8941 aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa gatacttgtg
     9001 cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa taaagcccct
     9061 gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat ttccagatta
     9121 gtaaatgtag taatctttgc tttctttttt ctcaatacta ataactaact tatggggtaa
     9181 acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga tttgatcagg
     9241 actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat tatattgtcc
     9301 cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga agcgatccat
     9361 ttccttgcca tcaatttgaa cgatggcata tttgatagca tcttcagttg tttcattctc
     9421 cttttctata gagaatacaa ataagggaat aaaagaggga ataatcaaaa tgccaataat
     9481 tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg atatctggct
     9541 aaacatatta gaatttttcc atggaaatgg tgaaattttt cggaaattat ctgcattaag
     9601 agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca tcttcatacc
     9661 aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt ccttcaattc
     9721 gggttttttt gtataatcat ttgacactca attttaggta aactagctac ccaataattt
     9781 aatgattcaa tacttttatg gctacccgat gtttcaaaga tcgtcgaaaa atcactatgt
     9841 tgggcaaacc aaagaaaccc ttttgctctt gtaatattgc caggaaaatt tttatccgta
     9901 agatgggcaa atttttgtgc gtcaaggagt aaccgatttc gatagataaa acttgagata
     9961 ccatattctt ctgtttcagg aatatgattt tgatagccaa gctctaactc tttgaaccag
    10021 cccattgaat aggaagtctc atctatatta aaaagatttg tatttagaat tttcgataag
    10081 tctacttcac ctttaattgt cctaattatt ttggcattgg actgtaactt agcaattaga
    10141 ccgttaattt tgtaaatcgt cttggttgac actaagtcac tattatttaa aagtaatatg
    10201 ttacaacact cgaattgatc aattaacaag ccacgaatat cctgttcttc ttcaactaca
    10261 ttaccttcag cttttctttc ctgaatcttt tcacccgttt cgaaattatc ccaaaaacgt
    10321 tttacatcca ctactataat aatggcacca atctctaatc gttgggtcaa atcaatgtct
    10381 aaagggtttt tagctaatat aattgcttga ataattggaa ttggtttgct gattcctgaa
    10441 gcttcaataa caatttgatt caagtcagaa aattgcacta attgatctag ttcaacaatc
    10501 aaatcttcgc gtaatgtaca acaaatgcat ccattcggca tcgaaacaag tttttctcca
    10561 gttcttttaa aacctttctt gtcgactaat accccatcaa catttacttc ccctatgtca
    10621 tttacaatga cagcaatttc ctttccgtga tcaccgtaca gaatatgatt tagaagtatc
    10681 gtttttcctg aaccaagata gccagttaag attgtaatag gtattttttc atagggaacc
    10741 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
    10801 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
    10861 ttgtcatctt actatactca aacgagaaag aatctttctg cttcacgaag aaggattttc
    10921 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
    10981 aagaaacaag aaaaaaacta atcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
    11041 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
    11101 ttatttgtcg attttcagtg gtcgccagag aaaatctcga atcgtttgaa gcatgaacat
    11161 atggactttt caattagttt tatcacgatc taccgtgcaa tttgcaatga tcttttcgat
    11221 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
    11281 acaagacata caaaaaatca tgtcgagtct cgtgggaaga tttgccttac tcattcgatc
    11341 catggccatc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
    11401 gtagctggca aaactagtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
    11461 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
    11521 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
    11581 gaattctcca atcatttcgc agttacagaa gtattgaata atatgccctt ttattttcca
    11641 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
    11701 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
    11761 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
    11821 ttgataaagt gttgcgcttg atttgacaat tcaagaaata ataaaaagca gatcaaatcc
    11881 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
    11941 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
    12001 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
    12061 tgtactccca tttaatctca tttgctttgc aaattgataa cgccgataag catcaatatt
    12121 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa gattggattt
    12181 taatgggcca tcatttcttg agcaatctca tgctcagata atgaagctgg atagaaagta
    12241 ggccaattat gcatttcatt ataaagtttt tcttgacttt ctccaccaaa gaaaatatgg
    12301 aaatgctctg ccttacttgg tgcaattcca tgatcgctaa attgaatata tttaaaacga
    12361 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
    12421 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
    12481 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
    12541 taataatctt tgtactcggc agctgtcatg tctttattga gttttgcttt gtaatcgaaa
    12601 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
    12661 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
    12721 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttctct
    12781 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
    12841 tttgtaagac tttcaagagg attcaatact tccaatttta tcccagcttc attggccaaa
    12901 gtttttgcga ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
    12961 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
    13021 ccagcaatag gaacttgtat caatccataa tccaaagcta aatatccaaa ggctgcatgt
    13081 tgtgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
    13141 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
    13201 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
    13261 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
    13321 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
    13381 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
    13441 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
    13501 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
    13561 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
    13621 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
    13681 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
    13741 tattttgttt atgttctttg ataattttta atgccctttt tctagttttg atattcgggc
    13801 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccctaat
    13861 caatacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
    13921 ttcatccaac atttttccaa ttagaataat agtggtttgg tctttttgtc gagtggtgta
    13981 ttgaaaatta acctgttcgt ttaccccttg tatggcaact ataaaatctt gatcttgcaa
    14041 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccggtcaat
    14101 taattggaat aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
    14161 ctcatgttct tgatgatgtt gatgatagtg tttgccatta tagtgatttt ctgatgaatc
    14221 actttcaaaa actaagggat gattgaattt ttccaaatta taaaaatcct ttttttgaat
    14281 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
    14341 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
    14401 tgctatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
    14461 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
    14521 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
    14581 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
    14641 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
    14701 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
    14761 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
    14821 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
    14881 tatttcactt ctttgaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
    14941 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
    15001 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
    15061 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
    15121 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcaata cattgaaata
    15181 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
    15241 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
    15301 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
    15361 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
    15421 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
    15481 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
    15541 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
    15601 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
    15661 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
    15721 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
    15781 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
    15841 ttaattcctg acgtttatct gcgtactttt caatcatcgc catttgtttc tttgctttag
    15901 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacatctc
    15961 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
    16021 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
    16081 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaagctctg aaaaataaga
    16141 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
    16201 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
    16261 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
    16321 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
    16381 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
    16441 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
    16501 ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa
    16561 ctatttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
    16621 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
    16681 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
    16741 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
    16801 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
    16861 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
    16921 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
    16981 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
    17041 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
    17101 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
    17161 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
    17221 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
    17281 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
    17341 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
    17401 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
    17461 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
    17521 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
    17581 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
    17641 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
    17701 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
    17761 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
    17821 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
    17881 gtaaatttga caaatcgtct gcaatttgca ttcttaaatg cgcactttct ttataagtac
    17941 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacga
    18001 acgatactgt aaaataaagc caaggcggct ttacattttc taaaactaat tcgtctatca
    18061 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag ctagaaatag
    18121 atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt ttataaaaac
    18181 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
    18241 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
    18301 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
    18361 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
    18421 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
    18481 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
    18541 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
    18601 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
    18661 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
    18721 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
    18781 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
    18841 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
    18901 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    18961 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    19021 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    19081 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    19141 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    19201 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    19261 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattcct
    19321 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    19381 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    19441 agaaccaatc caattttaaa attaatgaaa aagcctaagg caactgataa aacaggtgtt
    19501 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    19561 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    19621 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    19681 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    19741 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agacttaaga
    19801 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    19861 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    19921 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    19981 ttccgtaata gcgtaaatta gagatgtaca aacagataga aggtaaacta cgttctattt
    20041 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    20101 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    20161 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    20221 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    20281 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    20341 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    20401 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    20461 gattccagat aataatccaa aaatccctgg ggatatgctg tgaccaaacg ccccaccaat
    20521 actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat
    20581 aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta
    20641 aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca
    20701 ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg atcatgagtg
    20761 gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata
    20821 ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat
    20881 gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga
    20941 tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact
    21001 atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt
    21061 tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg
    21121 gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc
    21181 cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt
    21241 tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact
    21301 tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc agtttttaac
    21361 aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg atcaattaat
    21421 tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt attttttaaa
    21481 tcacaaagta agtcagcaag tttcttagtc gctttgaagt ccaaatttga agacggttca
    21541 tccaatataa ttaatttaga gtttagcgat aaactacatc caatagaaat tttctgccgt
    21601 tcaccacttg atagcgaaaa aatgttttta tctaatatat ttgaaagatc taataattca
    21661 gctaattcat tgattctttt atccatatgc tcttttgtaa atccaaagtt ttccatcgaa
    21721 aatactaact cactcgttgt atttgttgta aagaattgtc ctctaggatc ttgacttacg
    21781 tagccaatat tttttgatat actttcttgt gttaaggatt gtagcgagtt attttcaaat
    21841 aaaatttccc caaccaattt tccgtcataa agtcttggaa taagtccatt cattaacttt
    21901 aatagtgtgg atttcccact cccagaagaa ccagtaatca atgtaagact accttttttt
    21961 attgaaaaat taaggtcaac aagcgcttgt tccttaacat ccttatatga aaaagaaaca
    22021 ttgttaaatt caaacaattc tttcacctca aatcaaaata cttaagaaaa aaatagtaac
    22081 ggaaaacagg atgatatagt ctaataagct catgtttgtt ttatagtaag aacttctttt
    22141 cacaggagat tctatcccct ttgttattgc attagcggtc atttcttcag caatcatagt
    22201 tgcacgtata agaacgggaa ctaacagaaa ttccaaatat tgcagaggct gaaaaaaaga
    22261 caagcgaatg tttctcattt ttaaagattc tttaatcatt cttatttcag aacgataagt
    22321 cggaaagaat ctgatcacta ctgtaatact taacattatt tttttcggta aatgtatttt
    22381 ttctaaagag gataataatt cattgctagt atagacacta accaacaacc atgagatcat
    22441 tacgaccggc aacattcgaa atacaatata taaggatgtc ccaaaaaata tagtaaaaaa
    22501 ctctatcttg atagcatcaa taattttcat tactaaaaac agaactgaat aaatcaagat
    22561 gaaataaata catgtcttta ttttacgaaa catcaatact aaaactgtag caataatgaa
    22621 acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt
    22681 taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat
    22741 cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt gcgatcttat
    22801 caaaaagcat aactgagaac aattctcaat gcaagctttt ttttaattaa ctcagtaatt
    22861 caatccaccc aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt
    22921 ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt
    22981 ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat
    23041 ctagacaaca gactgtcttt tttgtcacaa atgatgtaat tttttcaaca atatcttgat
    23101 atttggttcc agctgattgg aataaaacca aatcgaatat acgtttattc tcaaaaatat
    23161 aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct atatctatat
    23221 tttctaagtt taagaggtat ttggtaaact cttcattata catattaata agcgcattaa
    23281 tatgcggctg aataatttct tcaaataacg cttccttaga agtaaaatgt ttataaaaag
    23341 cacccgtagt tacgcctgcg gtcaagcaca gttgccttat cttagtttgc tgaaatccta
    23401 ctttttcaaa atggcttagt ccactttcta ttaatttttg gtgagttgta atataatctc
    23461 tggtttctat gggtacttcc tcctaataag atgaattaac tatattatct tacacaggac
    23521 ttgaagtttc aatcgttaac gtctattact gaactcattc cttagagcta aactaatgag
    23581 aaagagcaaa ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat
    23641 cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg
    23701 ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa aataaagcct
    23761 tttacttaaa accttttttt gtaatcgtca ataaattttt tccctttatc agttaattga
    23821 aatcctatat ctaaatagtc ctcatatcta agtttagtca gcatctcact tacagatgct
    23881 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
    23941 aattcctgaa ttgctagtaa ataaattagt atagtagttg ttttaatttg taccatacct
    24001 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
    24061 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
    24121 agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt
    24181 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
    24241 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
    24301 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
    24361 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
    24421 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
    24481 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc
    24541 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
    24601 ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag
    24661 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
    24721 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
    24781 ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct
    24841 ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga
    24901 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
    24961 taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
    25021 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
    25081 aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt
    25141 ttttaaactg tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat
    25201 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa
    25261 tagataatga taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga
    25321 agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc
    25381 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata agatatgaaa
    25441 taaatccgtt gagctatttg caactcctat aaggataact cctaatgcta aaaaagaact
    25501 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
    25561 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
    25621 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
    25681 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
    25741 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
    25801 attattcccc ttgtatatta gagttagggt tttaaccttc cggttttgat tggataaccc
    25861 tatgaaccat cgttatttta atttggtatt aactcatttc ttttgagata atatatttat
    25921 tggattgaga ggctaagccg ttcccccctc tccaataaat atagtatctc cataggcttt
    25981 ttttaaattc tcttcattaa atacagattc tacacttccg tgagcaatta gatttcgatt
    26041 caaaagaata atattatcaa aatacttctt taccttgctt aaatcatgat gcacaattag
    26101 cactgttttt ccctgatgtt tcaattcttt taacgtatcc ataattattc gttcactaac
    26161 taagtctatc cctacaaatg gttcatctaa aaatatgaaa tccgcatttt gagcaagaca
    26221 tcttgctaaa agtacacgtt gaaattgtcc tccagataat tctcctattt gatgattaga
    26281 atattttccc atatcgacct tttccaaagc ctttgaaact ctttgccatt ctgcattttt
    26341 aattctttga aaaactttca ttccggcata tgttcccata gatacacatt cttttacttt
    26401 tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca tatgcaatct tcaccagttt
    26461 tttattgata tcttcattat taatggagac ctctccctga tgagggataa tatttaacat
    26521 tgctttaagc aatgtagatt ttccagcacc atttggacca ataatacctg ttaaagcacc
    26581 ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa tcgttataac aaacattaag
    26641 ctttttcact tctaacataa ctttctcctt ctaaatgata atgattatca cgatcatatg
    26701 gtatacaaaa attatatttt agtcaaccct aaattttata atttagattt ttttctgttg
    26761 ttgaagatta ggatgtcaaa attatttgaa actactcgta tagtaaatag taagtaattt
    26821 tcaaaacacg aagaaaaaca aaaagaaaaa aggaaacgcc gaagcatttc ctttcagtga
    26881 tttaatgaga acaacacgat ttgacttaga gtctcaaaaa gtcgaatagg tacactcatt
    26941 taagtgtctc cattcgactt tttggggatg agctggctat tgaggtccgc ttaccctatt
    27001 gaataaatac aagattgggt atttctaata agtccgccct tcatattggg gtgatcgggg
    27061 cgaacatgcc atcttttcat ataccacctc ctcgacactc aataatcaaa gattctcgtt
    27121 aatcttcttc gatgcttgca atgatgtttt caatattcat ataacgaaaa tcttcttgtt
    27181 tgatataaac gatctgagcg gtacattttt cctttttgat gatttctttt actttttcct
    27241 cagaacgtgc gacctgcggc gttaaaagta aaacgtcata gtctttataa ttcagttttt
    27301 cttcaacgtt tcggatcgtt gcataatcta tctcatacga tagatttttg gctctgatcg
    27361 tttcaagcat ttttgatacg agcagcgacg cactgattcc ggtctcgcaa ataatcaata
    27421 gatccttatg cataataccc tcctgttttt agcctgcaaa gccaattggt gaaaatgcca
    27481 cataagttaa aataacaatg actaccaatg ctatagcagt caccttggtg tatttccaag
    27541 gctcgatatc gacctttgta tcgaatgagt tcaacacaaa ttctccatca ggtttcaatt
    27601 tggaaaccac catcaaaatg ataatgtcca gaacgaataa tgccgtcaaa acataaagga
    27661 aatggatatt ttggaaaagc atttttgata gtgcatacaa gacgatatgc acggcaatcg
    27721 tcgtttttgc cccaaaagca gttgcttttt ttgaataaaa ggcaaagaga atgatcacga
    27781 gcagcggcat gttgtatagt ccattgaatt cttgaacgaa ctgatacaat ccagccggtg
    27841 caaaagaaat gaatggcgct acgataatgg agataatccc cacaacgacc gttactgttt
    27901 taccagcacg agcgatttgc ttatcatttg ctttcttatt gataagcggt ttataaaaat
    27961 ccagtgaaaa caaggtcgat gtcgcgttca aagctcccgc aaatgatgat aagatcgcgc
    28021 caaagataac agcggcgaaa ataccaaaga tcattttagg caatacttca ctgactaaaa
    28081 aaggatacgc gttatctgga tttgacatta aactatcgcc aaaaatattg cgagcgacga
    28141 tccctggaaa gacaaggaat aacgcaccta agattttgaa gacgccgact aggaaagcgc
    28201 ccttttgtgc ctcagctaaa ttcttgccgg acaatgcttt ttggacgatc atttgattgg
    28261 tacaccagaa atacagattg ttaaacagca tccctgtaaa tagtgtcggc catggaacaa
    28321 cagccgagtc gatcgatccc acagagttaa gcagccatgg cgtgtttacc acaacgtgtt
    28381 cgatcccgcc aacaaagctg ccgtcaccaa gcttcattaa gcctaaaatt gggaccagca
    28441 gaccacaagc cagcagaccg actccataaa tggagtcact attggcactt aacgacaagc
    28501 cgccgatcag cagatagagg atgccgacaa taccaattgc catcgctaca attatgatcg
    28561 cgacgatcgg ctggacgccc agcagctcat caatatggaa gatcttattg aacaccagcg
    28621 atccagaata aagaaccacg ggtaagaaag aagtcatata ggtcacaata aataagatcg
    28681 aaataattcg tttggtaacc gtatcataac gaatctcgat gaaatctgag attgtattga
    28741 tgccgtattt aaaatatttt ggcaagaata tcacagccaa agctacgatc gcaatcgtag
    28801 cagttacttc ccaggccatt acttccatcc ctgcatgata actttgacca ttttgtccga
    28861 ctatttgttc ggtggacagg ttcgtcatga tgatcgtgcc ggcgattggc aaagcagtta
    28921 agctccgccc tcccatgaag aacccttctg atccagaagt atcgacactt ctggatctag
    28981 aataggcaaa taaccataca cagatgacaa tcactaggaa actgattata gaaaaaagat
    29041 tcatcgtcta gctccttttc aatagattaa agaccggtga cctctcgaat gtagcttctg
    29101 gctttgactg catattcaaa tggattggct ttagccgggt cctgttctgc ttcaactacg
    29161 atccagcctt catagtcgct ggcatggatt gctgtccaga ttggagtgaa atcaatcatc
    29221 ccatcaccag gaaccgtgaa gacgcctttc ttcacgccgt ttaagaagct gtctttttcc
    29281 ttttctactt cttcagccgt ttcttgacga atatctttga aatggatgtg tttgatacga
    29341 tcttgatgac ggttgtaaac cgcgattgga ttttcaccag aaaagaccaa atgtcccgta
    29401 tcgaacaata atttaacttt gtctggatca gtcatttcca ttaaacgatc gatttcttcc
    29461 gtcgtttgaa cgccggtacc catgtgatga tggtaaacga tctccatatc cttttcgtgg
    29521 gccaaatccc ctaatttttc taatccttcc gccagttttt tccactcttc atcagtgaaa
    29581 actggtttat catcaaagac tggcgctgtc ataatccctt ggatactatg accctgctca
    29641 gctactacga tgactttagc gcccatcgca tgtaagaaat cccgatgtgc tttgaaggct
    29701 tcggcagttt cttcaaaagg tttagttgtc agaaacgcac tgaaccatgc actggcaacc
    29761 tttaagccgc gaatattcaa atattgttcc aacacctctg gatcttttgg atatttgttg
    29821 ccaatctctg ttccggtgaa cccagcaagg gccatttcac tgatacattg ttcaaaggta
    29881 ttttcactac caagctcggg catatcatca ttggtccacg cgattggtgc gatggctaat
    29941 tgaatttgtt tggtcatatt ctcacactcc tgcttatttt tttagttgta ctaaactctt
    30001 cgtatcgaat gattcttgac aagccaacgc gatcttagtt gattgcaatc catcttctgc
    30061 attaaccgct ggttgacgtt tttcttgaat acatgagaca aattcttggg cttcacgaat
    30121 gaaggcttct ctgaatcgtt ctgggaagtt ttgtgaacat ggacggatga tcccttgatc
    30181 gttcatgatc gtcacaagat ttttctcagg cgcttgcgct acccgcagca ttcctttggt
    30241 tcccatgatc tctgtttcga catgatagcc atgggctgcg tttctgccgg caactagtaa
    30301 agctattgtt ttatcgctca ggctcatcat tgcggctcca gtttcaagct caccagcctg
    30361 atccaattct ggatacgccg catttttccc aatcgcccac acttgatcga cttcttggcc
    30421 ggtaaaccag cgaaccaaat caatatcatg gatcgccata tctaagaaca agccgccgct
    30481 gtcgttattc atggcaaatt tgacaaagct ttccaagcct tcgctcggat cgatcccata
    30541 acaacgaatc agtgtgattt cgccgatttc gccatcttct accattttct tcgcatactg
    30601 atacgactca tcatagcggc gcatgaagcc taacatgaag acctgttcag gatgctcttc
    30661 gatgactttt tgggtctttt taatatcttc aacttcaagg cctaatggtt tttcagtaaa
    30721 gacatgcttg ccattttcca tcgccaagcg gacttggtca cagtgaaagc ctgatggtga
    30781 aacaataaag actgcatcga tcacggaact tttgaccatt tcttcatagc tggaaaaagt
    30841 atgcttgacg cctaatttct tctgtgcgaa tgctaattct gtttcaacta aactgcacgc
    30901 agccgtaagc tcacaacaac tgacacctgt tgctaaattt tgtgcatgta cttttcctaa
    30961 tcttcctaaa ccgacgatgc ctactctaat gttgttcatg aaatacctcc gttttctgag
    31021 aattactcat tgtatagtgc tggtcgttca tccatttgaa tcattacttt tgtttttgtt
    31081 tcacgagact catgacaagc catcatggtg atcgctgcta catacccatc ccaagcggta
    31141 ggaccgacaa ccgcatcatt tttgatcgaa tcgatccaca gcttgaattc ataatcatag
    31201 gcatccaaaa agcgggtctg ccaatctggt gaaacaggtg tgtaattttt ttcttccgat
    31261 ttaattcgtg tataggctgg gtccgtcatt cctacttctg ccgtttcgaa aaccacatca
    31321 catttaatat cgtagccaaa gcggcaattc acaaagattt ccaaatcaat cgttacaccg
    31381 gattttgttt gaaagatcag cagctgcgga tcatgcaaat cggcatggga gttgctggtt
    31441 tttttcggca agatcatttg aacagattca taatcttcat ttagcaacca gcgaagcaca
    31501 tcaatctcat gaatcgcggt gttgctgatc gccatcggtg ttgtgtagtt ttcatctgcg
    31561 ctaggatttc tatgggcaca atgcatcatt aatgcttctc cgtattgctt cgattcgatt
    31621 gcttctttca gttcaatgta gccgcggtca tagcggcgca tgaagccaac ctgaaccaat
    31681 ttcttcccat aagcaacttc cgcatcaacg atccggcggc agcctgctgc atccatcgct
    31741 aatggctttt cgcagaaaac atatttccca tgcttgattg cttctaacac atactgctca
    31801 tgggtcgggt cccaagaagt cacaacaacg acatccacat cttcagaagc gatcaatgct
    31861 tctccggttt caaagaattt agcatcgaca cgtttggcaa cctctttagc agcttttaca
    31921 ttgatatctg agactgctgt cacataagcc ccttgtgttt tctgattgat cctgtcgata
    31981 tgctcgatgc cgattgcacc tgttccgata acgcctactt ttactctcaa ttacttatcc
    32041 ccctgttctg ttgtttaagc acacatagta tgcgcttaca aaattattcc tgtgtagctt
    32101 ttattcttaa aaggtagagc cctctctttg ctccgccttt tactacccat aagcttgtac
    32161 gtataattcc ttaatttgac tatgactagg caccttttta ttattttgcg gacttccgct
    32221 ggtcaacgca tccgcggcca ttttgtctaa ggctgccata aacgcttgct gatcaatacc
    32281 ataatctcga atcgttggga tcgacagctg ctcacagagc tcttggattt ttttaatcag
    32341 ataatccgca gcctcttcca gcgataaatc ttcggctgcc ggataaataa atctggcgat
    32401 cgtcgctaac tcagtctgaa tagcatcctt tgagaattca aaaaagaccg gcaataacat
    32461 cgcatttgat atcccatgag gaatgtgaaa aagtgccccc accggtcgac tcattccatg
    32521 aatcaatgtg actgacgcat tcgaaaaggc aatccccgcc tccgttgaag ctaagctcat
    32581 ttgttctctg gcttcagtgt cgtcgctatt ttcatatgcc tgagaaagcg tgttcaaaat
    32641 tttcttcatc gcttccagtg aaaatagttg cgttacttca ttggctttga ctgaaacata
    32701 agattccaaa gcgtgacata gcgcatcgac tcccgttgcc gcggttactt ttggtggtac
    32761 agttttcgta aaatcagagt caacgattga gatctgtggc atgaaaacga gatccttcat
    32821 catcattttg actgctgttt ttgtattggt aataactgcc acatcggtta cttccgaacc
    32881 agttccagca gttgttgaca ctgcaatcag cggaatgccc gcttgcagct gatcaaaatt
    32941 tcttgcatga atcgcttcga gacttaattg attagcagcc aaaacggcta ccaccttggc
    33001 tgtatccaga cagctgccgc caccaaaagc aatgatatga tcacattgat gcttctgaaa
    33061 aagcttcagc gcatcataaa cattttcttg cgtcggttcg gcttgaatgt ctgtatactc
    33121 cgctaattga tcatctgttt ttcccaacag ctccttgatc ttttgaagat gcccggtctg
    33181 ttccataacc gtatcactaa tgattaacgg cttgttgcct agttgttctg attcttccat
    33241 aagaaattgg attgttccgc cgccaattct taaattggca ggcattttca gttgaatatt
    33301 cacagaaaaa acctctctct ggcttatagc ctataacaga atcctcgaag ctctttaaca
    33361 gagcttcgaa gcaaatcttt aaatctcact ttgcgcataa cgtgctgtaa caactttccg
    33421 atgggtataa aactcaacac tgtctttgcc attggcatgc aaatcaccaa agaaagagtg
    33481 cttccagcct gaaaaggcaa accaagccat cggtgcaggc accccaagat tgacccccag
    33541 catcccagca tcaacatttt ctctgaagta gcgaatcgct gatgcgttgt tggtatacag
    33601 acacgaacca ttcgcaaatt cagattcatt cgtcagctca acggctgcca tcaaatcttt
    33661 tacccgcacg attgatgcga ccggggcaaa aatctcatcc tgccagatct tcatatccat
    33721 cgtgacatta tcaaaaatcg ttggtccaac aaaatagcct ttttctggaa tgttttcgcg
    33781 accatctagt accagtttcg ctccttcatc gacccctttt tggatatagt caaaggtccg
    33841 ctgctgtgca tcttttcgaa tcactggtcc taaaaagacc ccttcgtcca agccattgcc
    33901 gatcttgatc gcttttgccg cagccttaaa tttttcaaca aactgatccg cgatttcctc
    33961 ttccaaaaca atgacggagg ctgccatgca gcgttcacca gcagaaccaa aagctccgcc
    34021 aataaggtcc ttgactgccc gatcaatatt ggcatctttc agcacaacaa catgattttt
    34081 cgctccagtc aacgcttgta cccgttttag attttttgtt ccttcacggt aaacgtattc
    34141 accgacattt tttgatccta caaagctgat gccttttacc agtggatctt gcagcaatgt
    34201 gttgacaaca tcgtgggcac catagacaac attaaaaaca ccgctaggaa agcctgcttt
    34261 ttcaaccagc tcaacaattt tctccatcaa caatggtgtt ttttctgaag gcttcagcac
    34321 caccgtattt cctgccgcca gagccatcgg gaagatccag aagggcacca tcattggaaa
    34381 attaaacggt gtgatccctc cgattactcc gattgggtaa cgataattcg tgatctcaat
    34441 atcagtcgcc gctgttgata aagaatctcc catcattaaa ttggtgattc ccgctgcgtg
    34501 ctccacattt tcgattcctc gttgaacttc tcccagtgac tcggacaatg atttcccatt
    34561 ttccagcgtg atcaaacgcg ccaattcact tttatgatct gttaaaagct gatgcagctt
    34621 gaacagataa cgtccgcgaa caggaacagg aacttttttc caagtttgaa acgcagcatc
    34681 ggcagcttta actgcttcgg ctgtctctgc tttgctagac aacggtacat tcgccagcgc
    34741 ttcttctgta gccggattca ttacttcttc aaactctgtt gagcgactag cgacccaacg
    34801 accatcaata taatttctta aaaatcttgt cattta
//
LOCUS       DACJGB010000024        34770 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.024, whole genome shotgun sequence.
ACCESSION   DACJGB010000024 DACJGB010000000
VERSION     DACJGB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.024"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(226..1113)
                     /locus_tag="IYI34_001228"
     CDS             complement(226..1113)
                     /locus_tag="IYI34_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0559106.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="IYI34_001229"
     CDS             complement(1110..1979)
                     /locus_tag="IYI34_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0559107.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="IYI34_001230"
     CDS             complement(2116..3402)
                     /locus_tag="IYI34_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0559108.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="IYI34_001231"
     CDS             3675..4253
                     /locus_tag="IYI34_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAR0559109.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="IYI34_001232"
     CDS             4253..5182
                     /locus_tag="IYI34_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0559110.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="IYI34_001233"
     CDS             5179..6915
                     /locus_tag="IYI34_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR0559111.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="IYI34_001234"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="IYI34_001234"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAR0559112.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="IYI34_001235"
     CDS             complement(7811..8320)
                     /locus_tag="IYI34_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAR0559113.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="IYI34_001236"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="IYI34_001236"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAR0559114.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="IYI34_001237"
     CDS             complement(9621..10712)
                     /locus_tag="IYI34_001237"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAR0559115.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="IYI34_001238"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="IYI34_001238"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAR0559116.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="IYI34_001239"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="IYI34_001239"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAR0559117.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="IYI34_001240"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="IYI34_001240"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAR0559118.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14675..15649)
                     /locus_tag="IYI34_001241"
     CDS             complement(14675..15649)
                     /locus_tag="IYI34_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAR0559119.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15630..17513)
                     /locus_tag="IYI34_001242"
     CDS             complement(15630..17513)
                     /locus_tag="IYI34_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAR0559120.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17531..19756)
                     /locus_tag="IYI34_001243"
     CDS             complement(17531..19756)
                     /locus_tag="IYI34_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAR0559121.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19871..20905
                     /locus_tag="IYI34_001244"
     CDS             19871..20905
                     /locus_tag="IYI34_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0559122.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20972..21985)
                     /locus_tag="IYI34_001245"
     CDS             complement(20972..21985)
                     /locus_tag="IYI34_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR0559123.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22063..22251)
                     /locus_tag="IYI34_001246"
     CDS             complement(22063..22251)
                     /locus_tag="IYI34_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAR0559124.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22691..22819)
                     /locus_tag="IYI34_001247"
     CDS             complement(22691..22819)
                     /locus_tag="IYI34_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAR0559125.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="IYI34_001248"
     CDS             complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="IYI34_001248"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAR0559126.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24306..25361)
                     /locus_tag="IYI34_001249"
     CDS             complement(24306..25361)
                     /locus_tag="IYI34_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAR0559127.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="IYI34_001250"
     CDS             complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="IYI34_001250"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAR0559128.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="IYI34_001251"
     CDS             complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="IYI34_001251"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAR0559129.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="IYI34_001252"
     CDS             complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="IYI34_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAR0559130.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="IYI34_001253"
     CDS             complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="IYI34_001253"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAR0559131.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="IYI34_001254"
     CDS             complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="IYI34_001254"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAR0559132.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33123..34682
                     /locus_tag="IYI34_001255"
     CDS             33123..34682
                     /locus_tag="IYI34_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAR0559133.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6335 g        11423 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac ctagtataga
    14461 cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc tgatagttaa
    14521 ctttgttttt cagaaaaata acaattggtt ttattgaaga aaatagtcat gaacaaaagt
    14581 atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc tttgtcttgt
    14641 aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag ccgatttcat
    14701 ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag ctattgatac
    14761 ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt
    14821 cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa
    14881 atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta tccgtattat
    14941 ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg ccgcattctt
    15001 ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct tcgacaccac
    15061 cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa aattttgctt
    15121 tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct ttttgtccat
    15181 aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa attgtcactt
    15241 gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt
    15301 cattacttga atattgatta tttactggag caatcggcaa atcgatcttt ttattgtcca
    15361 ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac ataccggatt
    15421 taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg
    15481 tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg cctcttgtat
    15541 aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg ccgaagacga
    15601 ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata ataatatctt
    15661 acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt ccctgagaat
    15721 tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg
    15781 attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc tccttggtac
    15841 atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga attccccatg
    15901 tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag gtagtcaatg
    15961 atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc ttcgaatgct
    16021 ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt tttgaagtaa
    16081 tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga ccaaaaaaga
    16141 atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc
    16201 tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc cattttttgc
    16261 catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc ttggtcgcat
    16321 aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt gatctttgca
    16381 ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg tccttttttc
    16441 ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt gaagtagatg
    16501 attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata ctccgttact
    16561 cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg aaaatgcccc
    16621 cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt taacggatat
    16681 tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag ctgactgttt
    16741 ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca tttgtcatct
    16801 aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac taagaagaca
    16861 tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc agctgtactt
    16921 cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt taaatcaaat
    16981 tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa ccaaataaac
    17041 attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag accttcttct
    17101 aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga aaagatccct
    17161 tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg tccctcccac
    17221 ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat atgactagga
    17281 acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt ttttggtcgt
    17341 accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg tttaagggag
    17401 gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg tgcatttgat
    17461 tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt catacttttt
    17521 tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat
    17581 cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc ttcgaggtct
    17641 gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg aacgctggtt
    17701 gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta ggagacacaa
    17761 acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa tggccatact
    17821 ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc tttttttctt
    17881 caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata gctgtttctc
    17941 ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct gatacatgag
    18001 ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc atacgctcca
    18061 ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac ccggcatcaa
    18121 accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca gttgtcaaat
    18181 gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttctcct tgtctctgtg
    18241 ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat ttgatgtagt
    18301 caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc cgaacatcta
    18361 accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta cctggctcct
    18421 gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc atttcaggtt
    18481 caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt tcacgtaatc
    18541 catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg tccgcatctc
    18601 tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc tttgcttcat
    18661 caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc cagttattga
    18721 ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca cgtagcaact
    18781 catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg atcatttcag
    18841 gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg ttgatcccac
    18901 cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg ttgccggaat
    18961 aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga tgaactaaag
    19021 caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat acttttgttc
    19081 cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa
    19141 tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc gctttttcta
    19201 gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc actgctctat
    19261 cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa
    19321 tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag ccttcgatct
    19381 ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag tctgatccat
    19441 tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga gaagagtatt
    19501 cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca tttcctgaaa
    19561 atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact ggtttgccaa
    19621 aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa agaaatgtat
    19681 ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta tctgtttttc
    19741 tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact tttttttata
    19801 attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt tttttgagga
    19861 gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac
    19921 agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta ctcggcaacg
    19981 gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat atatcaaaaa
    20041 gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag aacaggatat
    20101 ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc atttagggat
    20161 gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag atcagttagg
    20221 tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt taaaacaaaa
    20281 catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata tggtgtatgc
    20341 agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg gtcataaaag
    20401 aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga agcagatttc
    20461 tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc acttagaaaa
    20521 atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa
    20581 tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg atccaatggc
    20641 aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag agattgttgt
    20701 agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga ccagtgttca
    20761 aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc gaattgacgg
    20821 tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta gggaaagttt
    20881 tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc gtttcttcat
    20941 actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca aaatccaatg
    21001 tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat tcatgggtaa
    21061 tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat tctgtcaacc
    21121 gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca gttatatacg
    21181 ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat tgctcactcg
    21241 ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa aacttcataa
    21301 atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg caataagaaa
    21361 tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt ttagcatacg
    21421 catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca ctatctccac
    21481 caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct aataaatctt
    21541 tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc gctaattcat
    21601 aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca aattctttga
    21661 cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga acaggaacag
    21721 gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac ttggactcat
    21781 acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt tttttatcag
    21841 tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt ctgggggaaa
    21901 gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag aaccatccga
    21961 taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta agcattctga
    22021 ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat tttccccaaa
    22081 cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa acaaaacatt
    22141 ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat ccaaaggcag
    22201 atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca tttcgccatt
    22261 tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc tctaaaagaa
    22321 caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca ttttcttgag
    22381 taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc gaataataat
    22441 ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa ataaaaatct
    22501 ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga atgacgttca
    22561 ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat cgccaaaata
    22621 atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc ttagtctttc
    22681 tttagcacgt ctatttatta aagattttag ggccttttct catgttttta cgatcattct
    22741 taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc aattctttca
    22801 ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta attcgatatg
    22861 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
    22921 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
    22981 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
    23041 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
    23101 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
    23161 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
    23221 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
    23281 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
    23341 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
    23401 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
    23461 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
    23521 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
    23581 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
    23641 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
    23701 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
    23761 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
    23821 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
    23881 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
    23941 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
    24001 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
    24061 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
    24121 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
    24181 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
    24241 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
    24301 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
    24361 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
    24421 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
    24481 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
    24541 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
    24601 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
    24661 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
    24721 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
    24781 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
    24841 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
    24901 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
    24961 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
    25021 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
    25081 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
    25141 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
    25201 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
    25261 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
    25321 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
    25381 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
    25441 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
    25501 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
    25561 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
    25621 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
    25681 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
    25741 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
    25801 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
    25861 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
    25921 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
    25981 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
    26041 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
    26101 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
    26161 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
    26221 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
    26281 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
    26341 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
    26401 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
    26461 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
    26521 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
    26581 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
    26641 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
    26701 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
    26761 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
    26821 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
    26881 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
    26941 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
    27001 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
    27061 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
    27121 tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc gcgcttaagg
    27181 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
    27241 tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag cggataccat
    27301 cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa acagccacac
    27361 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
    27421 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
    27481 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
    27541 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
    27601 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
    27661 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
    27721 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
    27781 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
    27841 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
    27901 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
    27961 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
    28021 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
    28081 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
    28141 atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt
    28201 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
    28261 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
    28321 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
    28381 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttccgt
    28441 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
    28501 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
    28561 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
    28621 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
    28681 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
    28741 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
    28801 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtctc
    28861 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
    28921 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
    28981 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
    29041 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
    29101 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
    29161 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
    29221 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
    29281 ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat catacatctg
    29341 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
    29401 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
    29461 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
    29521 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
    29581 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
    29641 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
    29701 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
    29761 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgatag taaaaccaag
    29821 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
    29881 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
    29941 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
    30001 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
    30061 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
    30121 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
    30181 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
    30241 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
    30301 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
    30361 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
    30421 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
    30481 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
    30541 gatcaaatca ggatattgtt cctcgattgc tactaattct gcgtattctt tatcataaac
    30601 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
    30661 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
    30721 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
    30781 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
    30841 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
    30901 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
    30961 ctgttttttt agcgaataca ggcgtttttt taggcgtagg acgcatgcct tcgatttcca
    31021 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
    31081 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
    31141 catatgcgag acggcgttct tcctccagtt cgctttcttc catcaatgct cgagataaag
    31201 gaaaaacccc ctcttctaga ccaatcagga atacaactgg aaattctaat cctttggctg
    31261 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atgtcggaaa
    31321 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
    31381 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
    31441 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
    31501 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
    31561 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
    31621 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
    31681 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
    31741 aactgattga atcttgagga tttaccagca cattcaaata cgctaaaata tctttgattt
    31801 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
    31861 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
    31921 aattccgatg attgcttcga atctcttctt gcatccggtc tacgataaaa cgggtttcgt
    31981 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
    32041 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
    32101 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
    32161 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
    32221 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
    32281 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatacg
    32341 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
    32401 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
    32461 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
    32521 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
    32581 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
    32641 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    32701 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    32761 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    32821 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    32881 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    32941 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    33001 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    33061 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    33121 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    33181 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    33241 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    33301 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    33361 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    33421 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    33481 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    33541 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    33601 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    33661 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    33721 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    33781 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    33841 gcatcttagg ttttatttat cagaatgtct tgctggcttt gccaaaattt tacaaccgat
    33901 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    33961 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    34021 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    34081 cttacggtgg gggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    34141 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    34201 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    34261 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    34321 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    34381 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    34441 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    34501 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    34561 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    34621 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    34681 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    34741 attctataaa aaaagaagag gctgggaaaa
//
LOCUS       DACJGB010000025        34393 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.025, whole genome shotgun sequence.
ACCESSION   DACJGB010000025 DACJGB010000000
VERSION     DACJGB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.025"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(44..1936)
                     /locus_tag="IYI34_001256"
     CDS             complement(44..1936)
                     /locus_tag="IYI34_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559134.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(1933..3660)
                     /locus_tag="IYI34_001257"
     CDS             complement(1933..3660)
                     /locus_tag="IYI34_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559135.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3676..4326)
                     /locus_tag="IYI34_001258"
     CDS             complement(3676..4326)
                     /locus_tag="IYI34_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0559136.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4530..4826
                     /locus_tag="IYI34_001259"
     CDS             4530..4826
                     /locus_tag="IYI34_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAR0559137.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5018..5638
                     /locus_tag="IYI34_001260"
     CDS             5018..5638
                     /locus_tag="IYI34_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAR0559138.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5730..6896)
                     /locus_tag="IYI34_001261"
     CDS             complement(5730..6896)
                     /locus_tag="IYI34_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR0559139.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(6908..7261)
                     /locus_tag="IYI34_001262"
     CDS             complement(6908..7261)
                     /locus_tag="IYI34_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAR0559140.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7389..8411
                     /locus_tag="IYI34_001263"
     CDS             7389..8411
                     /locus_tag="IYI34_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAR0559141.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8850..9284)
                     /locus_tag="IYI34_001264"
     CDS             complement(8850..9284)
                     /locus_tag="IYI34_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559142.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9394..11253)
                     /locus_tag="IYI34_001265"
     CDS             complement(9394..11253)
                     /locus_tag="IYI34_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR0559143.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11371..12531)
                     /locus_tag="IYI34_001266"
     CDS             complement(11371..12531)
                     /locus_tag="IYI34_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAR0559144.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12838..14262)
                     /gene="araA"
                     /locus_tag="IYI34_001267"
     CDS             complement(12838..14262)
                     /gene="araA"
                     /locus_tag="IYI34_001267"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAR0559145.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14278..14976)
                     /locus_tag="IYI34_001268"
     CDS             complement(14278..14976)
                     /locus_tag="IYI34_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAR0559146.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(14933..16594)
                     /locus_tag="IYI34_001269"
     CDS             complement(14933..16594)
                     /locus_tag="IYI34_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAR0559147.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16812..18212
                     /locus_tag="IYI34_001270"
     CDS             16812..18212
                     /locus_tag="IYI34_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAR0559148.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18464..19543)
                     /locus_tag="IYI34_001271"
     CDS             complement(18464..19543)
                     /locus_tag="IYI34_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0559149.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19629..20588)
                     /locus_tag="IYI34_001272"
     CDS             complement(19629..20588)
                     /locus_tag="IYI34_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAR0559150.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20600..22123)
                     /locus_tag="IYI34_001273"
     CDS             complement(20600..22123)
                     /locus_tag="IYI34_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAR0559151.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22133..22697)
                     /locus_tag="IYI34_001274"
                     /pseudo
     CDS             complement(22133..22697)
                     /locus_tag="IYI34_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(22703..23566)
                     /locus_tag="IYI34_001275"
     CDS             complement(22703..23566)
                     /locus_tag="IYI34_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0559152.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23580..24476)
                     /locus_tag="IYI34_001276"
     CDS             complement(23580..24476)
                     /locus_tag="IYI34_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0559153.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24651..25922)
                     /locus_tag="IYI34_001277"
     CDS             complement(24651..25922)
                     /locus_tag="IYI34_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0559154.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(25912..27429)
                     /locus_tag="IYI34_001278"
     CDS             complement(25912..27429)
                     /locus_tag="IYI34_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAR0559155.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27627..28517
                     /locus_tag="IYI34_001279"
     CDS             27627..28517
                     /locus_tag="IYI34_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAR0559156.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="IYI34_001280"
     CDS             complement(28590..29414)
                     /gene="nadE"
                     /locus_tag="IYI34_001280"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAR0559157.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29426..30898)
                     /locus_tag="IYI34_001281"
     CDS             complement(29426..30898)
                     /locus_tag="IYI34_001281"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAR0559158.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31535..31897)
                     /locus_tag="IYI34_001282"
     CDS             complement(31535..31897)
                     /locus_tag="IYI34_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAR0559159.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32120..32872
                     /locus_tag="IYI34_001283"
     CDS             32120..32872
                     /locus_tag="IYI34_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR0559160.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33012..33935)
                     /locus_tag="IYI34_001284"
     CDS             complement(33012..33935)
                     /locus_tag="IYI34_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAR0559161.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(33950..34222)
                     /locus_tag="IYI34_001285"
     CDS             complement(33950..34222)
                     /locus_tag="IYI34_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559162.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10148 a         7166 c         5911 g        11168 t
ORIGIN      
        1 cttttgtcac agcccctaaa aaaaataata aatttgtact attctatgaa gcttcttctt
       61 cagaaaactg actgttataa agatctgcat agaatccgtc tttttccatc aatgtatcgt
      121 ggtttcctgt ttctacgatc gaaccatgat tcatcacaat aatgttgtct gcatcgcgta
      181 ttgtagacaa gcggtgagca acaacgaaac ttgtccggtt ttccaatagg cggttcatcg
      241 ctttttgaat cagtatttct gttcgtgtat ctacactaga tgttgcttca tcaaggatca
      301 ggacatctgg atttgctaag aatgcccgcg caattgtgat tagctgacgc tgcccttgcg
      361 aaatattgct tgcatcctcg tttaatacag tgttatatcc ttctggcaat ttacgaacaa
      421 aatcgtctac gtgagcagct ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt
      481 tcccatattt gatattgtca tagatacttc ctgtaaacag ccaagtatct tgcagtacca
      541 ttgaaaactg tgcgcgcaat tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac
      601 gaatactacc tccagaaaca tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc
      661 ctgcaccagt cggacctaca atagcgacca tctcaccagg tttgacatcc aagttaaaat
      721 cagtcattag caagttatct ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt
      781 atggtgtatc tttttcttct ggaaggccag atttttcatt ggacatctct tcttcatcca
      841 acacttcaaa tacccgttcg gctgaagcaa ctgttgattg gatagtgttc atcaaacttg
      901 caagttgagt gattggttga gagaactgtg ttgtatattg caagaacgct tgaacatccc
      961 ctaatgtcat gttcccattt gcaactttta ctccgcctag tacagcgacg aacacatagc
     1021 ctagattttt aacgaaattc atcaacggca tgatgattgc cgaaataaat tgagctttcc
     1081 aaccagcggc atacagattc tgattttctt tttcaaatac ttcctggtct ttttcagtat
     1141 gattgaatgt tttaacaatg gtatgaccac catatgtttc ttctacttgg ttattcaata
     1201 aacctaaact tttttgctga tctgcgaaaa acacttgtga tttcggcgct acgatcatca
     1261 cgatgatcaa gcttaaaggg atcatcaata acgcgactaa cgtcaattgc cagctgattg
     1321 aaagcatcat cacgatgacc ccaatcaatg tgactgcact agttacaaat tgagtcaaat
     1381 tttgctgcag cgtgcttgcg atgttatcca tatcattgat tgctcgagac atgatatccc
     1441 catttgtatg tgtatcataa taggaaatcg gtacacgatt cattttggct tccaaatcat
     1501 gacgtaattc ataaactgtt cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt
     1561 tgaatacagc agaaatcaaa tacatcacga ttactgtcgc aatgatctgc gcgattttat
     1621 caaagtctat cggaaatttt gttacttgtt ggccttgttg catctgttga gcgccagcca
     1681 ttacaccttt gaatatttct gtggttgctt tccccaagac ctttggtgtt tggatttgga
     1741 agatcacggc tgctattgct agtacaaata cagcgatgat cgcaaccata cgtttagaca
     1801 tatagccaaa caatcggcgt accgttcccc agaaattttt aggcttttca ccttttacac
     1861 cgagatttct gccaggtcca ccatgtcctg gaccgcctgc tggtccatga gagggacgtg
     1921 tgtttgtttt actcatgcta agtcctcctc tcttagttgt gaatgaacga tttcttgata
     1981 agtttcgttt gttgctaata gttcttcatg tgttcctttg ccaaccagtt ttccttcatc
     2041 tagcacaagg atcatatctg catccattac tgtacttact cgttgagcaa ctaaaacggt
     2101 gatactttct ttcatattcg ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc
     2161 caacgctgag aaggaatcat caaacacata gatatctgct gttttgacta atgcccgtgc
     2221 aattgccaga cgctgtcttt gtccaccaga gaagtttcct cctccttgtt ccacatggct
     2281 atctaagcca tcttccatct ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt
     2341 ccaaatcatt tcatctgttg catccggctt accatactgc atattgtctc ggatcgttcc
     2401 agagaacaga actgcttttt gaggaacaaa gcccattaat tctcgcaaat tatattgagt
     2461 catgtcacta atgtccgttc catttatttg gatcgaacca gattcgatgt catataatcg
     2521 agggatcaaa ttgactaagg tcgttttacc tgaaccagtc ccccctatga ttgcgacgat
     2581 ctcgcccgac ttggcttgga aatcaatatc ttccaatgca agtttttctg ctccatgata
     2641 tcgataattg acatgattga aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt
     2701 aggattttca gggtcatgga taccgatttt ttcatccaac acttcattaa tacggtcagc
     2761 tgaagcttgt gcacgaggca ccatgataaa aatagcggaa agcatcataa agctgatcaa
     2821 gatctgcatc gcataagtca taaatgcaat cagattaccg acttccaaag tcatatctgc
     2881 tatgtagtgt ccaccaaacc aagtgattgc tacgtttgta ccgctcatga tcaatgtcat
     2941 cattggcatc atcaatgcga cgatcgtatt aactttgatt gccgtatttg tataatcttt
     3001 attcgctaaa tcaaaacgat tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct
     3061 gacccctgtc aatccttctc gaaataccag gtttaaacgg tcagttttct tttgcatact
     3121 tttgaataat ggaacagcaa agtacatgat tccgccgatc aaaataatca ttactgggat
     3181 gacaactaag aagatttttg tcaactgatg atctttttga taagcaagta tgctcgcgcc
     3241 aatcaacgtg attggtgcat tgatcatcat acgtaaaaac atttgtgtaa ccatttggat
     3301 ttgagtcaca tcattggtcg ttcttgtgat caaagaggcc gttccatatt tatcaaacgc
     3361 atcttttgtc agactctctg attttttata gacatccgta cgcaattgct tacccagttt
     3421 ctgtgattca cgagtagcaa aaaatgtatt tgcgatagca gctaagatac tgatcaaaga
     3481 aaagccgatc atcacaaaac ctgtatgcca aatataatcg acatctcctt gcgctacccc
     3541 tttatcaata atattggagg taagtgtcgg caaatataaa tctgccagta cttggatcac
     3601 catgaaaact gcagctgcaa ttgctgaagt caaggagatc cgttttacga tttttagcat
     3661 ctagttccct tctttctatt tatatactcc gtttttcagc caagaaagtt tcaaattgaa
     3721 ttcactgatt gcttgctcta ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg
     3781 cgcaattgtt gtaaatacag tattcatcac taattggatc agtaattcca attcatgatc
     3841 attttttact ttcagcttcg tccgatcgat caattcaagc aactcttttg catcttgttt
     3901 gtgttcttta tgtggatcgt gaggcgtttt ttggcgactc gtatgaggag cgacttttct
     3961 tgaggcacgg taatccatgt tcataaacaa gttacgataa aaagaagcat tctcgccttc
     4021 caaaatctca taaatcattt tagaaaaata ggattcgaat cctttgaata gatcaccatc
     4081 ggattctttc attagttgta acatatcttt agagctgtca cgtctcattg tctcaaagta
     4141 ataaaaataa agatcctctt tatcttcaaa atactgatag aaactgcctc tagggatatc
     4201 cgctaatttg acgatattag caatcgaagc ttcttttaag ggtactctag aaaactcgat
     4261 tctcgcagcc tcgattagtc ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt
     4321 cggcatatgg attctccttt ctttcaagat gacacattgt catatgacgc tatgtcatta
     4381 taacacctcc tacctgtttt gcaagacatt tgcatagttt ttttcattat catccgatac
     4441 attaaaaaag tgataagagg aatccttcca tttgaattaa tgagagttgc ccattaaaat
     4501 agccacatac taaacaaagg atgagataaa tggcccaatc aattagtgtc caagacttca
     4561 ataaattgcc tctcgatgct gacagcatcg ttttagatac aagaaatacc ttagattaca
     4621 caaaaaacca cttaagccac tcgctttcta tccccgctgc tatcttgcca agacaattaa
     4681 aaaatctaga tccaaagaaa ctttattata ttctaagcta tacaggcagg cgatcagaag
     4741 cattagccga tcaacttact gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg
     4801 aagctcttca gtcgagtgtt gcttgaatat tgcttgttag attcaaaaga ggctgtgaca
     4861 aaaaatcgtg tcacagcctc ttttataccc tgtctttttt tagttattta ttcttcaacc
     4921 ttatcaatat gacttgatag ccttttcttt ttcgagtaaa ctgattttgt tagtcatagt
     4981 tataacaatc atagcaataa aaggagaagg taagcatatg gcatctgttt tagtagtcaa
     5041 aggtcatccc ctaactgcag aagaatcgcg cacagttaag gcattgacgt cttttttaac
     5101 aagttataaa gaaaatcatc cagatgatga agtgactgtt ttggaacttt atcgcgatga
     5161 tattccggaa atcgatgaag aattattatc aggttgggaa gcattacgtg ctggtgcaga
     5221 attcacttcc ttatcagaaa gtcaacaaca aaaaattgct cgtttcaatg aattgactga
     5281 tcagttcctt gctgctgata aaatcgttat cgcaaacgcc ttatggaatc taaatatccc
     5341 aacaaaatta aaagcttggt tcgataccgt caatgtagct ggaaaaacgt tccgctatac
     5401 agaaaacggg ccagaaggat tagtgacagg taaaaaagca ctgcacatcc aatctaatgg
     5461 cggtgtatac aacggacaag actttgcctc tcaatatgta aaaggtattt taaactttgt
     5521 aggcatcgat caagtagatc agctattcat cgaaggaatc gattatgacc ctgaccgtgc
     5581 agatgagttg atgcaaaatg cattagataa agctgctgct ttaggtaaat cattttaaaa
     5641 attgagataa atgcatacaa aagagacaga ggaaaaaatc ttcaaagcag catttgtgcc
     5701 ttggatattt tcccatctgt ctctttttgt tattctttct gctttttcaa cggattttct
     5761 tcataaagag aagagattgt tgtgtaccat tcaaaaacat gagtaatcac tactttagct
     5821 gctgcataaa caggaatccc caagatcaca ccgatgattc cgaacaattt cccagaagtc
     5881 agcagaacta ataaaatcgt tactggatgg attgctaact gattccctag tactaatgga
     5941 gagatcaagc gtccttctat tgtctgctct actgcaaaaa cgatcagtac tttgatgatc
     6001 atgaatggac ctgctacaat ggctaaaaag atagccggaa tcatcgctaa aaatgatcct
     6061 aagtaaggga tcaggtttaa aaaacccgca ataatcccca acgtgacagc atactctaaa
     6121 ccaatgaccg caaaaccaat cataaacatg attgccacag caaaagccac agttagttgt
     6181 cctcttatat aagaagaaac ttgactattc atttcactca gcacattcaa cgtcggtttt
     6241 tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta attgtttacc gtctttcaat
     6301 agataaaata agataaacgg catcgtcagc agagccacta aaatagtcgc taccgcgccg
     6361 acaaaacttc caagactctg aactgtactc ttagatatat cttggatcat atcgcctgcc
     6421 gaattcatga acttatcacc tgctgtttcc aactgttcgc gaaattggtt gaacagcggg
     6481 tcccgcaaaa tctcggttaa tttattatca atcgtatcta catattgagg gaagttactg
     6541 atgaagctca ctgtttgttc ctgaatctta gggatgatca ctacgctccc ccatgcgatt
     6601 agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa gccttggtac ttttcgcttt
     6661 tcaaagtaat caactaccgg attcatcaag taatacaaga ttcctgctaa aatgataggc
     6721 agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca aatatgaaac ttttgtaaat
     6781 aaaaagacga tcagcaatat taacaataca acaagtaagg ctgtgactac ttgattattt
     6841 aaaaaccagc gccaaaacca cgataccggt ctgttcttct tattttttcc ttccataatt
     6901 cctcctgtta gttataaaaa atttggcttc ccaaaccaat agcaggcagt ctatgcggat
     6961 gaagatagat tccatttgca atttgtcctt cttctggaca agcatcaaaa atcaatgata
     7021 cgtgagctac tgtctttaaa ttcccattgg ccatcggacc gacataatcg atcgtatata
     7081 cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga aaaaggttcg tcttttgata
     7141 cttcttgaat caccgaatat tttcgtaagg cagctgttgc tgttttatcg aacaaaatca
     7201 gcatgggttc ttccttgttt attgcttctg tcccaatttc tgtaatcttt gcctgaatca
     7261 ttgcactctc tcctttaatt gctttcaaaa acagtataac acattctcat tctttagccg
     7321 tgtggaataa ttatgtgata gaataaaaac gaaaactatt tcattacaat taggaggaat
     7381 cagctcatat gatcgaaaag ataattttag gaacctatac gcgtcgcgaa agtaaaggaa
     7441 tctatacgat cgaccttgat acagaaaaag aagaattagc caacctttca ttagctgttg
     7501 aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg caatctttac acagttacta
     7561 gtgtggatgg attaggtggt gccggtgcgt acgataagga ttttcatttt ttgaatgcgg
     7621 taacagagtc tggcgctcct ttgtgttatg tagcggttga tgaagcacgt caattactat
     7681 acggggcgaa ctatcataaa ggtgaagtaa atgtctacca tatattagga gatggcgaac
     7741 tgaaagcagc agattcaata ttccatcaag aagcgacagg tcctcataaa aaccaagatc
     7801 atgcgcatgt ccactatacg gatctgactc ctgaccaacg attagtcgta tgtgatcttg
     7861 gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg caaattgaat cttgtaacta
     7921 ccttcactgc agagccagga acaggccctc gccatcttgt ctttcatcca aatggacaat
     7981 tcgcttattt atttggtgaa ttagacagca cagtccgcgt gctttcttat aataaagaaa
     8041 caggtgcttt tgaagaatta cagaaaatat cgaccttgcc agaagaattc gacggtgaaa
     8101 atggaggggc agctatccgc atttcaaatg atggcaagtt cctttatgct tctaaccgag
     8161 gacataactc gattgctgta ttcgcagttt ttgaagatgg ttcccacatt caaaacaagc
     8221 aaatcatctc aacagaaggg gattttcccc gcgatttcgc actagatcca agcaataatt
     8281 ttgtcgtgtg tgccaatcag aacaccgata atctgacatt atatcgccgt catcctgaaa
     8341 caggattact ggaattactg aaaaaagatg tctatgtacc agaatgtgtc tgtgtttatt
     8401 ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca agacgtatta gacagactac
     8461 gggaagaact aaatattcct ttctttgatg ggaaaatcga agataaagag tatagcgaag
     8521 aggaatatca gaaactcaaa gccgatttac agaactactt tgaacaatat gtacagaatg
     8581 tagaaaacta atcagctttc catactatct gaaaagaaca tcaggacaag acttttccgt
     8641 ctatcctgat gttcttttct acaaataaaa aaccgccgag atggttagac tcgacggaaa
     8701 aggagtattt actcgatttt gagtaagtac aaggttgttg ttggttatag gtatgtcatt
     8761 atcataaagg attttgatca ctctatcata aaggtaatat tacatttata ttagataact
     8821 ctagttttta ttgttatcaa agatttcttt tattttttca acaacaaatt agtcattttt
     8881 tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt ttgcttcaga tgtattgaat
     8941 agatcttcat ctacccctga ttcaagatcg atactttgga tcatttcttc acagcctgct
     9001 acatatgaaa gataagcttt ttcaaaattt ttatgaatgc ccattgcttt tgctggtgga
     9061 cgcaaagtac tgactttcat aagcattgcc cgatattttt ttgttccttc ttcaaataac
     9121 aggatgatct cttcaagagt cgatttgtcc agttcacttg tttttccttc atcaattgcg
     9181 tttctgatca cttcatattt cggatgcatt ccttctccga ttgcttctgt ttccttgacg
     9241 atttgatcga tggactgcac ataatagcca atattcggta acatgttcgt tttcctactt
     9301 tctccattgt tgttattatt tattctactt gtttttatcc tcaaatgaca agtacttgaa
     9361 aaaatttaat ggtttctata cagctcgcca ttcttattcg tgctcaaaga aaccatctgc
     9421 taaactatat tgtttccctg cgacaatgtc atattggatc aattgatatt cgtcgtaaat
     9481 ctcttgttgc gagcgattga attgcgcttc tttataccac tgtccgtttt gatagtacag
     9541 ttctcgttca aacgccggaa gttcctgttc taatgataaa agcagctgat aaaagccatt
     9601 tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa taaaacggac tggtgattgc
     9661 atcgtgagtc gtttgctttt ctaattctcc tttactgtcg aacataagaa attgtgtttc
     9721 atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct gaatagatcc ctggaagatg
     9781 atccccccaa aaaacaacta aagtccttct agaaagattc ttcaattctt ccgtgaactg
     9841 cttcagtgcc gtgctgctat aggaaatatc ttgcagataa ttctctaagt caagtgtccc
     9901 acttccatct gaagtttttg caggatagtc tagattatca tactttccat cgtagggcat
     9961 atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt ttttcttcct tgagaagtgt
    10021 cattacttct ttgtaagctg actcatcaga aatatatgga ttattctcta tcgtatccgt
    10081 ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct aatgtctgat aaacatcctc
    10141 tcttttatac atcgaagtat tgtatggatg gatcgctgtc gtctggtatg attgtgcgtt
    10201 taacgctgaa acgatagaag gaagctgatc catttttggt acaagcatcg tgtaaggggt
    10261 ggtgagttgc gcattaaata acgccataga aaaaccagtc aatgcttcaa attcgatatt
    10321 tgctgttcca ccgccatagt tttgagaaag catattgcca ctatatgttt gatcagctac
    10381 ttcataataa tctgccaatg gctcaccaga tacttctacg ccgtttaatc gactaggatc
    10441 tgagaaactt tcactcatca cgaacacgat gttcggcgac tcttcctcta cagttttatt
    10501 tttttcatcg gctagcttct ggtatttttc agtgatttct ttgatctttt ctttcgaata
    10561 tccttcaggt tcatccattg gttctacctt cagattgtac aaaaacccac cgataaaacc
    10621 agtgttatag tagttcattt tctggctgta aggtatccat agtgccgttt tattatatgc
    10681 ttttcgcaac aaattatcgg gattgttgaa attactgata taccctagca gtccaattgc
    10741 cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt ccttttttca ggcttcgaaa
    10801 catgtaccag caaaacaatc ctatcacggt tcctgcggca gctaagatca ctgtaaacag
    10861 catagtgccg gtcatatctt tcagcaatcc gatttcagtg atcatcttca aatcgtctgg
    10921 ataaatcggt tcttgccgat aactcatttt catataattt gcaaaaccta atacaccgat
    10981 ggctacgaca tacgtaaggg aacctaaagg gacagatcct gctagtgcaa tcagaaaaat
    11041 aagaataatc agcaagacga gacaggctaa gaagaatttt tccgtatgcc aagaaaaagc
    11101 aaatttcaac gcaaggtcta cggatagatc attttgacac cactgcaaaa ataaattact
    11161 aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt ccttttaaaa tctttttctg
    11221 tttggtcgaa agtctgctta tatatctttt caaatgcttg ctccttcatt tctatcaata
    11281 aaagaatgtt gcttctctta ttttagttgt acgaaattcg aaagtaaaga acggaaagaa
    11341 gaattaatga aatccaaata ttctctttct ttatggcctt tctggtttct ttaataagcg
    11401 gacctttcgt actccagaaa ttttttctag ctgttgtttc acttgattga gctgttcaaa
    11461 atcagtactg tctatgtcaa tcaatatgta gacatgctca ttcttatggt tacgcgatat
    11521 atctgcgatc gctagctcgt tctcattcag cagagaaaat atctctgcta atatattggt
    11581 tttcttttga taaaatatag tgaatcgaaa cggcgcttgg aaaaacattc gtgcggatgg
    11641 ataattgacc gcttctctaa ccgtaccgaa caaaagataa tcttttaagg cttcaactgc
    11701 caaacgtccg ctgtctgcca aagcttcctg agtcgatccc cctagatgag gcaacataag
    11761 tatacggtca ttttctaaga actcttcttc tggaaaatca gtcaagtatt tagccagatg
    11821 ttgtttttcc aatgcttgca taacagcata tttatcaaca atttcacttc ttcctacatt
    11881 gataagtaca gcatcttttt tcattttttc tatattcgcc tgatcgatca atccttttgt
    11941 atcttctgtc aacggcagca tgactacgat gtaatccgag gtagaaagaa ctgtttctaa
    12001 atctgcttgc cgaacataat ccaactgggc atcgcggata gaataaccca agacatccat
    12061 ccctaagcta tagcaactta gcgctacttt tgtgcctatc gctccaagac caagaagacc
    12121 gatggttttg ccttgcaatt ctcgtcccac ataagcactt cttttgtttt ctgcctgttc
    12181 taaaatatta ggacctgtta gtgtttgtac catacgactt gcttcgatta tcggacggga
    12241 agataagagc aaacagcaca aaacaagctc ttttactgca ttagcattta cacctggtgt
    12301 attcatgacg atcgtcccgt tttcagaagc tttttcaacg ttgatcgtgt tcaccccaac
    12361 accagctctt gaaattgcta gcaaatctgg tgtaagccat tcatctttca ctttcgtact
    12421 acgaataact attccttcgg ctggcaaatc atctattatt ttgaaatatt ccccttgtaa
    12481 cttttctatc tcttgttctg gaatagcatc aatcaaatga acgtgataca taaaaccccc
    12541 tccttactaa caagtatctc gaaatttctg caattagaca actgtttacc atttgacttc
    12601 ctagaagcaa aaagagcagt tagagcagag taggctgagt gataaaaata aggctatgac
    12661 aagttactgc cataagcctt attttcgaga gcaaaatatt gagggtgaga tatcttgata
    12721 aatggtgttc cgttagctga cattgataat aagactaaat ttcaaaaaag ttgaaaagca
    12781 aatttcgtca atctcttctt atttcttaaa actaagtggc tttttcacaa cctcttttta
    12841 tctaataacc actgtttcca actcaactaa ttttgcccac gtttcaattt gatctgttgt
    12901 tagatttagc gagacaacgg tatgatgacc gccacctgcc tgaatccatg aatggacacc
    12961 ttcatggaaa ttcggtttag ttttccataa cacacgtgca acaggtaaat taggtgcttc
    13021 agtcgttggt tcaaaggctt ctacttcatt gatcagcagt ttataatgcg taccgaaatc
    13081 tgccatcgat acgactaccc cttctcctgc tttcccatca aatacaagac gcgccggatc
    13141 ttctctatct cccattgata aaggagaaac aacgattctt ggtttcgtcg cagcaagcgt
    13201 cggatctact tccatcatat gggattccaa gattgcttct tggccactag ctagctcgta
    13261 agtataatct tccatgaatc ctgtatcttt ccctcgagcc atgattttca gcaaccgatc
    13321 aatcgctgca gttttccagt cgccttcccc agcaaaaccg tatccttctg ccatcaaacg
    13381 ttgtacagct agtccaggta gttgtttcat tccatacagg tcttcaaaat tagtcgtaaa
    13441 agcgttatag cctccttctt ctaagaaacg gcgaataccg atttcctgct gagcttgtac
    13501 ttttacatgt tcctcccatg tcttttcttc ataatcacca tagtcgaagg tatacaattc
    13561 cttgtactct tcaaataatt gatcgacttc ttcgtctgat acgcgatcga ttacttgtac
    13621 aagatctccg ataccaaaat aatcaacagt ccaaccaaat tgaatctgtg cttccacttt
    13681 gtctccttct gtgacaccca cgtttctcat gttgtcacca aaacgagcca cttttattcc
    13741 aaaactttca ttataggcta ctgcaacatc catccaatca gcaatctctt tttggatctc
    13801 aggtcgtttc cagtagccaa cgacgatttt attttgttta tttaatcgag cattgatgaa
    13861 cccatattcg cggtcaccat gagcggattg atttaaattc atgaaatcca tatcgatcgt
    13921 tttccaagga atgctttcgt tatattgcgt agctaaatga agcaatggtt tttgtagaag
    13981 ctttgtcccg cggatccaca tttttgctgg tgagaaagta tgcatccaag tgatcacacc
    14041 tgctacttct tctcgataat tgacctcttt catgattttt gtgatcgtat cagcagtaac
    14101 tgctaattct tgaaggcgga ttggataagg caattggcca ttttcattca gttcatcgac
    14161 catttcttgt gcatgtttct ttacttctcg cagtgcttct tccccatata aatgctgtga
    14221 tcctacaaca aaccaaaatt ctttttctcc aatattcaac ataattagcc actcctttta
    14281 tttgttttgt ccgtaatacg cattttttcc gtgttttcgt aaataatgtt tatctaaaat
    14341 ccgttgaggc agctcttctg aaaatgaatt gagctgtctg gtaaataaat tcatcttgca
    14401 tacttcatct agtacgactg cgttcattac cgcactttgc gcatctttcc cccaagtaaa
    14461 tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag ggatcaattt tgcgttcttt
    14521 gaatgtttct atgataactt ttcctgtttc atattcgtac ccgctgtcga tttcctgttg
    14581 tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta tctgcatgcg tcgtccccat
    14641 cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc catgtcgagt gagtatggac
    14701 gattcctccg atttgaggaa atgaacgata taaaacagca tgagtaggcg tatccgaaga
    14761 gggattgagt ttaccttcaa tgatatgatt gtccaagtcc acaacgacca tgtcgtctgc
    14821 tgtcatctct tcataactga ctccgctagg tttgataacg aaatatcgtg tttcaggatc
    14881 aaaagcgctc acatttcccc atgtatattt gacgagtccg tgtttaggca agtcaagatt
    14941 tgcttggtat acttcttctt tcagttgttc cagcattttc tatcactcct ttttcttgca
    15001 attgatgaat cgctgttttt tcaatgtcga tcccctcacg gtaacgtctc ataaatatct
    15061 gatagcctgc tatttcttct gctgaaggtg tgatggtgat tccttggtct tgtccaaaca
    15121 cgtcttctgc caagaagttt tctaaagact tcccatgtcg atccatcata taggctgcta
    15181 ataacgcgat tccccacgct ccaccttctc cggctgtgtc catcactgta actggtgcct
    15241 ccatagcaga tgctagaatc cgctgtccaa cttctggtgt tttaaaaatc cccccatgcc
    15301 ccactaaacg atcaatttgg acattctcag atttcaatat atccattccg attctcattg
    15361 caccaaaagc actggctaaa tggatccgca tgaaattcgc taaatcaaaa cggctacctg
    15421 gtgtacgtac aaataatggc cttccttcat ttactcctgt aatgttttca ccggaaaaat
    15481 aaccataaga aagtaatccc ccgcaatccg tatcgccttc tagcgctttg ttgaataata
    15541 ccgtgaataa tttatctctg cttatctcca tacccaaaga attggtaaat tcttcaaaaa
    15601 tactgaccca agcattgata tccgaagagc agttattcgt atgaaccatg cctactagtt
    15661 ctcctgaagg tgtagtgacc atgtcgattt ccggatggac attttctagt tctttttcta
    15721 atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt ccctgtccct ttggctacac
    15781 tgttcgtagc gaccataccg gttcctgcat ccccttctgg cggacaaacc gggattcctg
    15841 gtctcaaatt tcccgtaggg tcgatcagat aagcccctga ttcgcttaac ttgccagctt
    15901 cttctcctgc tactagaact ttaggcaaaa tgtgatccaa gtcaatcgct acgtgcgctt
    15961 tttcgattaa ctgaccaaaa atatttttca tttcttgatg gtaggttttc gttgaaggat
    16021 cgataggaaa catgcctgat gcatctccta cgcctagtac tttctcacca gtaagctgcc
    16081 aatggacata acctgcaagc gttgtcaaaa aattaatttc tttcacatgt tcttctttat
    16141 tcaaaataga ttgatacaaa tgagcgatac tccatctctg cggaatattg tattgaaatt
    16201 tttccgttaa tattcgttct gcttcttcag tgattgtatt tcgccaagta cgaaatggta
    16261 ctagtaactg ttccttgcta tcaaatgcta gataaccgtg catcattgca ctgaacccaa
    16321 tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa tacttctgct gccatttttc
    16381 ggtaactgac gcgcagtcct ttccaaatat cttctaatga ataggtccag ataccatttt
    16441 ctagtttgtt ttcccactca taactaccag cggcaatagg agtaaaagat gaatcaatca
    16501 atacagcttt gatcctagtt gatccaaact caatccctaa agatgtacga ccatctatga
    16561 catctaacat actttgcgca tttacttcct tcataatgat ttaactccct ccttctcgtc
    16621 aaacctgatc aaaccgtctt tttagtagcg cttacaatga ttattgtata cttttgtacg
    16681 tacatgtcaa gcagttattt acaagaatac aataaaaaac acttgttttg tacgtacata
    16741 agttataaaa tgaaattgct tacaaaatct gatcaaccgc cttctcgaaa ttctagaagg
    16801 agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc gtttatttat ttttttggcg
    16861 catttggcgg cattctattt ggatatgata ttggtgttat gaccggcgcc ttaccttttt
    16921 tacagcatga ttggggtttg gcaggtaaag cttctttgat aggatggatc acgtcttcgg
    16981 tcatgttagg tgcgatcctt ggcggctctt tatctggtct tctttctgat aagcttggac
    17041 gacgaaaaat gattcttctt tctgcactta tttttatggc tggttctgtc ctttctgcat
    17101 ctgcccctca caatggttct tattttctga ttgcagcaag gattcttcta ggtttagctg
    17161 ttggcgctgc atctgcccta gttcctgctt atatgtctga aatggctcct gctagactaa
    17221 ggggaagatt gtcagggatc aatcaagtga tgattgcttc tggcatgctc ctttcctatg
    17281 ttgcagatta tttgctaaaa gggttgcctg aaacgatggc ttggcgcgtg atgttagggc
    17341 tcgctgctgt ccccgcgctg attttatttt tcggtgtttt agctctacca gagtcaccgc
    17401 gttttcttat gcaatctggc cgcttggaag aggcaaaaag agtattgaac tatattcgga
    17461 caccaaaaga agctgaacag gaattcgaac aaatccagct gaatgtcaaa caagaaaaga
    17521 caaccggaac ttcttggcat actttgtttt tagaaaaata tcgttcctta gtcattgccg
    17581 gtattggtgt agcagtattc cagcaatttc aaggagctaa tgctattttt tactatattc
    17641 cgctgatcgt agaaaaagcg actgggcatg cagcgagcga tgcattgatg tggccgatca
    17701 tccaaggaat cattttagtt gctggttctt tgctcttttt ggttattgca gacaagttca
    17761 atcggcgaac tttattgaaa ataggcggta gcgtgatggg actttctttc atcctaccag
    17821 ctgttttagg aactgtactg gatgctcata caaattcgtt gttgattctc ttgtttttat
    17881 gtatttatgt cgcattttat tcttgcacat gggctccact gacatgggta atcgtaggcg
    17941 aaatattccc actagctgtc cgcgggcgag catctggcct agcctcttct tttaattgga
    18001 ttggttcttt cctagttggc ttgctttttc ctgtgatgac tgcttctatg tctcaagaaa
    18061 tcgttttcgg catttttggg atcatctgct ttttaggggt tttatttatt caagagatcg
    18121 taccagaaac tcgtgggaaa tctttagagg aaatcgaaca aagtgcatct aaaaaaactt
    18181 atcctaaacg tatttctatc catcattctt aaagaggttg tgacagaagt ggtcagcttc
    18241 aagaaataag aaggaaccgc ttagtttcca gtcacaagga acaatttcaa aaaacagctt
    18301 ctcatgtttg atgaatattg tgacttgtga ccagttggta ggagaacacc gtttagtgga
    18361 gaggtggtga aagaaacgtt cagccttgag cgatgacaaa agctctcaca aacattgcga
    18421 cgagtaaccg taggagcaat aagaggcggt aaaaacccgt ctcttatttt tcgttttctt
    18481 tcactgtttc ccgttcaact agtacaggtt catagagaat atttggctgt gcctgtcctt
    18541 tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc caacttttct tttggatgcg
    18601 tcaatgttgt caatttttta ttccctaaat ggcttaaaga agaatcgtct gttcctacga
    18661 tcgataggtc atctggaata tgatagccat tttctgtcaa cttatctagc attaaactag
    18721 ctatttgatc gttatagcaa atgatgccat ctaaagcttg ttcttctttc aaccgttgta
    18781 ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa agtaatgata tcttccgtca
    18841 aaaaacgcac attatactgt tcacacgcgc tgataaaacc tttcatcctg tattttcctt
    18901 gaagatcgtc gatttttgtg atgaacaaca gacgctgatg tccttttttt atcaaatatt
    18961 ctgctgccat aaaaccgact ttcacatcat ctaaacaaat ataagaggca tttaactctt
    19021 cataagcagc attgatcatc accatcggaa tgccttgttc tctcaaagca acataaagtg
    19081 ctaagtttgg gttatactga ttacttttcg taggctcaac gatcaaacca tctaccccat
    19141 aagcaatcat tttcttcaaa cactctttct ccaattcgtg atcgttattc gtactggcta
    19201 aaagcaatga atacccagat tctcgtaatt cattttcgat tcctcggata atcgacggaa
    19261 aaatataatc agagagatat gtggtgatga caccgatcgt ttttgttctg ttcaatggtg
    19321 ttttttcttt cttatagctg tcatctacat atgtgcccga ccctttttct ttacgcagat
    19381 agccctcatt taccaacaca gcgattgctt gccgaatcgt atgtctgctg acgtcatata
    19441 tttcttgcag cttgaattct gagggaatgg ttgtatttac ttcgtattcc tcacgaagga
    19501 tcttttgttt tatctcttct gcaatatctt gatattttgc catttgcctt gccccttttt
    19561 atttgtacgt acatatatca taacgagtct gcaaggttta ttcaagcaaa aatcaaggtt
    19621 tcttgtgctc ataaggcata cggaacaggt tcaccaaatt ctggaaatcc ttgctcattc
    19681 cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt ctaacggatc ccctttttgg
    19741 ttcttctctg gtcgagcatg atagatcaaa atatcaaagc acccgtcttc tgaaaccgta
    19801 aacgagttgt gaccaggacc aaattgctta tttttttcag aagaaacgaa tactggctcc
    19861 gataatttat gccatgattc ttttaataaa agattcttgt gctcatcagc ccacagcagg
    19921 cccatggcat agttttcatc tgttgcacta ccagaatacg taatgaatat tttcccattg
    19981 cgagtaagga ctgctggacc ttcatttact gaaaaacccc gtttttccca ttcatactca
    20041 ggtatagaaa ggaggatctg cttccccttt aacgtccaag gattatccat ttcggataga
    20101 taaagattag aattcccagt aatttcagga tcttgttggg cccacacata atatagcttt
    20161 ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt cccttttcgt ttttatttgt
    20221 cccttttcta tccaatctgt attcattgga tctatatgct gattttcaag tacgaacatg
    20281 cggtgatgat gagattcatc acgtatagtt ttatgattgc ttgcagcaaa gtaaatatac
    20341 cattttccat tgatataatg gacttcaggt gcccagatca attcactcat cggtccttct
    20401 tcatgagcct gccatacaat caggcttgca gcatgttcta attcattcag tgactttgcc
    20461 cttcttaatt caatcacttg gtagcctgga accgatcctg taaaataata ataaccatct
    20521 gtatgtttat atacccaagg atctgcccgt tgccgaacga taggattatt ataaagtgtc
    20581 ttcatcataa caaatttcct cacattttct ttattctaat tacattccaa gagtattttt
    20641 ctatccctac atttaattcg tcatgttttt ctattgaaaa atagtttatt tcaggtttta
    20701 ttttttctgg ttcttcacgt gtatttttgt ccaacagatc gctactggtt aatgtgtagt
    20761 gctctccttg atctagcaaa ttgaaatcat gttcaatcgt tatcttttgt tgttcatttg
    20821 tgcgattgat caagaaaata gtcagctgat tgccgttatc tacgatcaca ctgtccacat
    20881 aaggaacatt ttttatcttt tcacaatcat aaacagggca aatgatttgt tgtttcaata
    20941 cttgaccttt agcatatttc gataagtgtt gcatcacata ataagttgga ttcctccatg
    21001 cttgtccttt agcatctgtc agtattaaag gtattgtgtt tactaacaat gattgacaac
    21061 taatctttac gcgatctgcg tgtcgtaaaa tagccagccc taatgaacca aataataatg
    21121 tatcttccat agtaaagtaa caccaatcga tgggactacc gacttcaaaa tccctatgtc
    21181 ttttttccgg ttgggcatgt acattccatt catcaaacgc tagatacatt gttttctcac
    21241 ttctcaaata tccttttaca taatcacaag tagcaacgat ttcttcaatt tgacgatcga
    21301 atcgataagc ttttgcaagg tatgtcgctg tatcatcacg ctcattttta ggaggtttag
    21361 tcaagtcttc ttcatcatac ttatcaatat agttatgcaa agctaaataa tctacattct
    21421 cataagcttc ttctaatgcg atacgatccc attcagggta cgatggtaaa cgtggcgtag
    21481 agcttccaac taagattgtt tcaattgaat catccactaa cttcatgact tttgaggctt
    21541 catttgctaa cctaccatat tcataagcac tcttagcccc cacttgccat ggaccgtcta
    21601 gctcattccc taaacaccat aatttaacgc caaaaggctt tttttgccca ttttttattc
    21661 ttaaatcact ccagaaggtt ccttttggaa aattacaata ttctactaaa ttttttgctt
    21721 catcaattcc ttttgttcct aaattcaccg tgaatatcgg tttcgcttgt acatgactga
    21781 tccatttaaa aaattcgtgg agtccaaaag aatttggttc tatacctcgc catgctaaat
    21841 caattttttt tggtcgttgt tctttcctac ctacagtatc cacccaatca taaccagatg
    21901 taaagtttcc tccaggatag cgaatgacac ctaattctaa ttcctttaca taagagagga
    21961 catcttgtct aaacccgtca ccatcagctg tttcatggcc cggttcataa atccctgtat
    22021 agacgacact tcccatatgc tcaacaaatg agccaaacag ccgagaactg atctctccta
    22081 ccgtaaaatc ctctctgatt tttactttca cttcttcgtc caacagcttt ccctaccttt
    22141 catttcctat agtcaagata atagatacaa gtttccatac gccaccagca aagataaaaa
    22201 taaaaggagg gaaaagaaat atgagcagta caaatcctaa taacaatacg caacaaagaa
    22261 tatttctcaa aaaatcaaaa aatactttcc atgcactttg ttcaaaaatt tgtaaaaatg
    22321 tgatatcttt gttttcagaa atcacatata tccaggataa aagaaacatg gaataaataa
    22381 gtaaaactac atatccacta attgaaagta cgattgtctg ttcttctaat aattgattaa
    22441 caagaaaata aataatgatc gttaacagac taaggaacaa gttcttacca aaatgttgct
    22501 ttattccttg aaagaaaaaa taaatgatat ttcctgatcc ctcacctttc cattgcgcca
    22561 ttacataaaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    22621 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta
    22681 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    22741 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    22801 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    22861 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    22921 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    22981 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    23041 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    23101 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    23161 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    23221 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    23281 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    23341 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    23401 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    23461 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    23521 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    23581 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    23641 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    23701 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    23761 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    23821 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    23881 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    23941 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    24001 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    24061 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    24121 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    24181 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    24241 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    24301 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    24361 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    24421 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    24481 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    24541 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    24601 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    24661 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    24721 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    24781 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    24841 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    24901 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    24961 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    25021 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    25081 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    25141 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    25201 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    25261 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    25321 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    25381 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    25441 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    25501 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    25561 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    25621 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    25681 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    25741 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    25801 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    25861 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    25921 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    25981 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    26041 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    26101 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    26161 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    26221 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    26281 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    26341 ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc
    26401 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    26461 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    26521 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    26581 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    26641 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    26701 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    26761 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    26821 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    26881 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    26941 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    27001 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    27061 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    27121 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    27181 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    27241 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    27301 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    27361 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    27421 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    27481 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
    27541 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    27601 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    27661 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    27721 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    27781 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    27841 tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt
    27901 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    27961 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    28021 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    28081 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    28141 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    28201 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    28261 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    28321 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    28381 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    28441 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    28501 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    28561 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    28621 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    28681 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    28741 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    28801 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    28861 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    28921 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    28981 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    29041 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    29101 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    29161 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    29221 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    29281 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    29341 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    29401 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    29461 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    29521 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    29581 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    29641 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    29701 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    29761 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    29821 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    29881 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    29941 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    30001 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    30061 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    30121 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    30181 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    30241 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    30301 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    30361 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    30421 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    30481 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    30541 gcccttccac ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    30601 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    30661 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    30721 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    30781 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    30841 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    30901 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    30961 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    31021 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    31081 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    31141 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    31201 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    31261 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    31321 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    31381 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    31441 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    31501 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    31561 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    31621 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    31681 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    31741 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    31801 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    31861 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    31921 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    31981 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    32041 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    32101 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    32161 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    32221 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    32281 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    32341 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    32401 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    32461 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    32521 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    32581 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    32641 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    32701 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
    32761 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    32821 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    32881 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    32941 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    33001 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    33061 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    33121 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    33181 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    33241 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    33301 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    33361 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    33421 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    33481 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    33541 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    33601 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
    33661 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    33721 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    33781 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    33841 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    33901 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    33961 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    34021 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    34081 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    34141 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    34201 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    34261 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    34321 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    34381 attattagtg aat
//
LOCUS       DACJGB010000026        33263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.026, whole genome shotgun sequence.
ACCESSION   DACJGB010000026 DACJGB010000000
VERSION     DACJGB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.026"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(41..229)
                     /locus_tag="IYI34_001286"
     CDS             complement(41..229)
                     /locus_tag="IYI34_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAR0559163.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="IYI34_001287"
     CDS             499..861
                     /locus_tag="IYI34_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR0559164.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="IYI34_001288"
     CDS             913..2595
                     /locus_tag="IYI34_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAR0559165.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="IYI34_001289"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="IYI34_001289"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAR0559166.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="IYI34_001290"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="IYI34_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAR0559167.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="IYI34_001291"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="IYI34_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAR0559168.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="IYI34_001292"
     CDS             6430..7434
                     /locus_tag="IYI34_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559169.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="IYI34_001293"
     CDS             7435..8379
                     /locus_tag="IYI34_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559170.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /locus_tag="IYI34_001294"
     CDS             8379..9341
                     /locus_tag="IYI34_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559171.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="IYI34_001295"
     CDS             9368..10282
                     /locus_tag="IYI34_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559172.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="IYI34_001296"
     CDS             10308..12089
                     /locus_tag="IYI34_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0559173.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /locus_tag="IYI34_001297"
     CDS             12437..13123
                     /locus_tag="IYI34_001297"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAR0559174.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="IYI34_001298"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="IYI34_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAR0559175.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="IYI34_001299"
     CDS             16733..17542
                     /locus_tag="IYI34_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0559176.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="IYI34_001300"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="IYI34_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAR0559177.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="IYI34_001301"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="IYI34_001301"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAR0559178.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="IYI34_001302"
     CDS             complement(21094..21546)
                     /locus_tag="IYI34_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAR0559179.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="IYI34_001303"
     CDS             21696..22646
                     /locus_tag="IYI34_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAR0559180.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="IYI34_001304"
     CDS             22639..23598
                     /locus_tag="IYI34_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAR0559181.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="IYI34_001305"
     CDS             23595..24350
                     /locus_tag="IYI34_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0559182.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="IYI34_001306"
     CDS             24373..25326
                     /locus_tag="IYI34_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0559183.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="IYI34_001307"
     CDS             complement(25970..26266)
                     /locus_tag="IYI34_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAR0559184.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="IYI34_001308"
     CDS             26621..26977
                     /locus_tag="IYI34_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAR0559185.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="IYI34_001309"
     CDS             26987..27886
                     /locus_tag="IYI34_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAR0559186.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28403..29134
                     /locus_tag="IYI34_001310"
     CDS             28403..29134
                     /locus_tag="IYI34_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAR0559187.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="IYI34_001311"
     CDS             29279..31306
                     /locus_tag="IYI34_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAR0559188.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="IYI34_001312"
     CDS             31414..32511
                     /locus_tag="IYI34_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAR0559189.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..>33263
                     /locus_tag="IYI34_001313"
     CDS             32818..>33263
                     /locus_tag="IYI34_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR0559190.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11138 a         5762 c         7123 g         9240 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gtt
//
LOCUS       DACJGB010000027        31488 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.027, whole genome shotgun sequence.
ACCESSION   DACJGB010000027 DACJGB010000000
VERSION     DACJGB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.027"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(79..546)
                     /locus_tag="IYI34_001314"
     CDS             complement(79..546)
                     /locus_tag="IYI34_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAR0559191.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(666..2204)
                     /locus_tag="IYI34_001315"
     CDS             complement(666..2204)
                     /locus_tag="IYI34_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAR0559192.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2284..2970
                     /locus_tag="IYI34_001316"
     CDS             2284..2970
                     /locus_tag="IYI34_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAR0559193.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3171..3698)
                     /gene="msrA"
                     /locus_tag="IYI34_001317"
     CDS             complement(3171..3698)
                     /gene="msrA"
                     /locus_tag="IYI34_001317"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR0559194.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3782..4459)
                     /gene="pgmB"
                     /locus_tag="IYI34_001318"
     CDS             complement(3782..4459)
                     /gene="pgmB"
                     /locus_tag="IYI34_001318"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAR0559195.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4456..5584)
                     /locus_tag="IYI34_001319"
                     /pseudo
     CDS             complement(4456..5584)
                     /locus_tag="IYI34_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(5584..7767)
                     /locus_tag="IYI34_001320"
     CDS             complement(5584..7767)
                     /locus_tag="IYI34_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAR0559196.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7764..8789)
                     /locus_tag="IYI34_001321"
     CDS             complement(7764..8789)
                     /locus_tag="IYI34_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR0559197.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8834..9661)
                     /locus_tag="IYI34_001322"
     CDS             complement(8834..9661)
                     /locus_tag="IYI34_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAR0559198.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9658..10725)
                     /locus_tag="IYI34_001323"
     CDS             complement(9658..10725)
                     /locus_tag="IYI34_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAR0559199.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10751..11587)
                     /locus_tag="IYI34_001324"
     CDS             complement(10751..11587)
                     /locus_tag="IYI34_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0559200.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11584..12453)
                     /locus_tag="IYI34_001325"
     CDS             complement(11584..12453)
                     /locus_tag="IYI34_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0559201.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12523..13806)
                     /locus_tag="IYI34_001326"
     CDS             complement(12523..13806)
                     /locus_tag="IYI34_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAR0559202.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13820..15505)
                     /locus_tag="IYI34_001327"
     CDS             complement(13820..15505)
                     /locus_tag="IYI34_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAR0559203.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15780..16805
                     /locus_tag="IYI34_001328"
     CDS             15780..16805
                     /locus_tag="IYI34_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0559204.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17400..21053)
                     /gene="rpoC"
                     /locus_tag="IYI34_001329"
     CDS             complement(17400..21053)
                     /gene="rpoC"
                     /locus_tag="IYI34_001329"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAR0559205.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21132..24749)
                     /gene="rpoB"
                     /locus_tag="IYI34_001330"
     CDS             complement(21132..24749)
                     /gene="rpoB"
                     /locus_tag="IYI34_001330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAR0559206.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25477..26445
                     /locus_tag="IYI34_001331"
     CDS             25477..26445
                     /locus_tag="IYI34_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0559207.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26463..27368
                     /locus_tag="IYI34_001332"
     CDS             26463..27368
                     /locus_tag="IYI34_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559208.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27365..28171
                     /locus_tag="IYI34_001333"
     CDS             27365..28171
                     /locus_tag="IYI34_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0559209.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28328..29296
                     /locus_tag="IYI34_001334"
     CDS             28328..29296
                     /locus_tag="IYI34_001334"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAR0559210.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29613..30137)
                     /locus_tag="IYI34_001335"
     CDS             complement(29613..30137)
                     /locus_tag="IYI34_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAR0559211.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30424..>31488
                     /gene="scm"
                     /locus_tag="IYI34_001336"
     CDS             30424..>31488
                     /gene="scm"
                     /locus_tag="IYI34_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAR0559212.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         8837 a         6801 c         5926 g         9924 t
ORIGIN      
        1 ttgacgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct ctttttcggt
       61 caagttcaac aacagttttt acgcgtcgat ctctggcgct ttgcccaaat gagcttggat
      121 catccagata cgtttttctg tggctgtttt gaattcgata aagatatctt gtgttggatc
      181 gtcgccttct tctccagaaa cttcgattcc tttttggtaa agatctgcta gataacgata
      241 gcctgcaaca agtgtttcca aacgttctgg aatagtacga tcccagcggc caatttcatc
      301 agggattttt gtgttatctg caaattcacg caatgtagaa taaggtgaac cgtctaatgt
      361 gatcaagcgt tcggatactt catctaactg agcgttcaaa tcatccatga actcatccat
      421 cattgggtgc aatgtaagga aacctggtcc tctcatgtac caatgtgttt ggtgaacaac
      481 aacagacatt tggcttagat ctgcaactaa ttgatttaat acttctttgg tttgttggaa
      541 tttcatcttt cttgcctcct aacattttct tacacttaaa ataatagtag actttcaatg
      601 aaaagtacaa caaaatcatt tacctgagaa cgattttcag ttcgcttttt tatccgtaaa
      661 gccaatcatt tttcagaatc agctaattct tctcgcagtt gagcaaattg ctgataagtc
      721 tgtgctaaat gcttttgtgt cgaaacaaat aatggataga ttttctgata ttttttatga
      781 tcagtttctt gtactccaac ttgctctttc aattctatca attcctgtgc ttcactgata
      841 ctttggatct ttccgatgct tctccagcct aaaatcacag ctcctaatgc ggatgcttct
      901 gtgacagtag ggaaaacaag cgggtaaccc agtacatcag ccatcatctg acgaaaaatc
      961 ggagattgag caaacccgcc tgtagcacgg atctctgtga gctgattttc ctggcaaact
     1021 ccttttaaaa tccgataaag gttgagacag atcccttcta gaatcgcctt catcatttct
     1081 ttttctgtat gttgccgctg taaaccgata aaactgccaa acgcatctgc gtcccaaagc
     1141 ggtgctcgct caccaagtaa atagggatga aaaatcagac cattggcacc cgctggcaca
     1201 aaagcgatag ccgaaagcag tgcatcatat ggattttctc ctttttcttt agcagattga
     1261 acgacttcct gtaaaaatgt atgccctgcc caatcaaaga tgccagcacc attgctagtc
     1321 gctcctccca ctacccaatg tttctgatcc agaacataac aaaacgtctc catttctgga
     1381 tggaactgag gctgagtagt aataaaacga agtgctccgc tcgttcctac ggtcaaagcg
     1441 gcgatcgtat caccaaaagc acctaaccct agattagaaa gcggcccgtc tgcccctccc
     1501 aaaacaaatg atatatcatt tggcaaacct agttctgctg ctgtttttga tggtagacct
     1561 gttacttgat aggtggaagg aaagacttgc ggcaattgct ctattcgaat gttcaagtag
     1621 tcaagcactt cttctgccca ttggaattca tggatatcaa aatatccagt tcctgaagct
     1681 gttccgtagt cgctgatcag ttcgccaaac aatctataaa agatatagtc tttgataccg
     1741 acaaaataag cagttttttt gaataattca ggttggctgt tttgcagcca cttgattttg
     1801 ttcaatggtg tcattggatg aatcggcgtc ccagtctgcc gataaaattc tttgccaagc
     1861 acggtatatt tcagtttttc tgcttcgtcc gctgctcggt tatctgccca agtgatcatt
     1921 cgagataacg gtgttttctc ttcatccaac aatatcaggc catgcatcgc agaggagaag
     1981 gagacaaatt gaatgattgt gtctgaaggc aactgtttaa ttacttcttt tataacggtg
     2041 cagacacctt gaaagatcaa ttcctgatct tgttctgcca tcccttgtgt ttcttggatc
     2101 aaagggtagc gaaaataagc ggatgctgct tcttgggctt catctgtgaa agcaactgct
     2161 ttggtttgtg tggttccgat atctaatccg attgaaacgt tcattttttc acctcaaatt
     2221 ttatgtatgt acaaaaattt agtttaagct tgcgtatctt ggttttgaaa agggggagat
     2281 ggcgtgttac tttattttat cattggttgc tgtatcggat catttttatg tttaaccgcc
     2341 cagcggattc cgttaggtca ctctattatt tatcctagat ctcattgtgt caaatgctgt
     2401 cgttcgcttt cttggtatga attgattccg attctctcga tcatcgtcca acgatttcgc
     2461 tgccgctatt gcaggtgtcg gttgcccttc tactatttgt tggcagaagc cttatgtggt
     2521 ggtctttttg cctggttctt tactttttct tccgcaagaa atttttcgac gttcatatgg
     2581 atgctttcgg ctttgctatt ttcgttgatg gatttatttt acttcatggt ccattctcac
     2641 acgctattct gttcttgggc aattttatgg attttttggt tgcaggccgg cgtcttccaa
     2701 tggcaaagtg tgctgttaac gtttatcgta ggcggtgtgt gccttcgcta tggtcaatcc
     2761 tatctcggag ctggcgatac tctgttgatg ctttcttgga gtggtggctt gtctctagaa
     2821 gagttgcttc agatcttgtt tcttgctagt ctgtttggaa tatccgtttt ttctgtttac
     2881 tttatctgtc ataaaaaaaa gttggaaaag ctgcctttca ttcctttttt gagcagtgcg
     2941 ttgctgattg tccttcatct taggtattga aaaaaggttg tgacaaacga cttgtcacaa
     3001 cctctgatat atagtgttct cttaccaaat ccttgtgact tggagctagg cgacaggttc
     3061 acaacctctc cactaaatgg tgttcaatca gctgaccctc agtattagct caaaatgctg
     3121 gttttacaac ctctgatata cggtgttcca taagtagctt tttcggcttc ttatttcttg
     3181 acgccccaat gtcgacgaag gaaacctgca cgtccagaac cttctcgata aagattgtaa
     3241 tggagcttgt ttttcttgta ataatcctga tggtaatctt ctgcaggata aaattcgctt
     3301 cttggttcga tttttgtcac gattggctta tcgaatcgcc cgctgtcttg caatgcttgt
     3361 tttgaacgct ctgcaatatt tcgttcttct tcatcattat agtagatcac tggacgataa
     3421 gaatcaccgc gatcagcgaa ctgtcctaat gcgtcagtcg gatcggtttg ctgccaatag
     3481 atttccacca actgatcata agaaataact tctggatcat attcgatctc tactgcttct
     3541 gtatgccctg ttgtgcctgt tgttacttgt tcataagttg gatttggtac atgcccgcca
     3601 gtataaccgg atgtcacaga aatgatccct ggctgctctt caaatggttt gaccatacac
     3661 caaaagcatc cgcctgcgaa aactgctttt tttgtcatat aaaatccccc ttttttctca
     3721 tcataccttg atttccaaaa ggaggataac gttttgcttg tgacagaaaa aacggaaact
     3781 attatatacg tgatacggct tctgataaat gccaaaacga acgtacttga tcaaaaacgg
     3841 ttaaatcagg aatcacatat tcagcagaag gaagatttat gttttcacct attcccaata
     3901 catgcatgtt accagccaaa gctgcttgac aaccagcttc tgaatcttca atgaccaagc
     3961 atgcatttgg cggtacattc aattgctgtg ctcctttgag aaaaacttct ggatcaggtt
     4021 ttgcttttga tacttgcgtg ccgtcaataa ggacatcaaa ataacttgtc aaattcgttt
     4081 tttctaaaat gaggcgtgca tttttgcttg ctgatcccaa tccgatcttg atgtttgttt
     4141 ttttcaagta ctcaagaaca gctattgcac caggcaaaac ggatgtttca tccatttttt
     4201 gaatcgcttg aagatataga ttgttttttt gctcagcaaa ggctgatttc tctttttctg
     4261 agaatttctg ttccattttt ccaaatgaca gcaatcgctc tagtgactcg gttctggata
     4321 ttcctttcag ttgctcgttt tctttctcag tgaatggaat ggacagctgg ttagctaaaa
     4381 ccagccacgc ttcgtaatga taacgagctg tgtctacaag aacgccatct aaatcgaata
     4441 gtgctgcttt gattttcatc gattacctcc ttgaaaatga acgacttgaa gtaaagatgc
     4501 gatgttttta gctgtgcgat gaagattatc agtggcttgc tgtaaagctt cttgtaaagg
     4561 cacagctgca ggaaggatcg gaaaaacggc cgaaatgcca acatctgctg ctttttcgct
     4621 tcctttacca acacttccgc aaatagctat cacaggaacg ccctctggta ctcttctagc
     4681 aatcccaact ggcgcttttc cttttagact ttgtccatct agacgtcctt cgcctacgat
     4741 tacgaggtct gcttgctgta cccgtatcga actccagtaa atccaatacg tagtcgatgc
     4801 cagagactat tttccctgaa gcaaatgcta acagtcctgc acccattcca ccaccagcac
     4861 tactgcccga tgcctcgatt acttgagggg caaatctcat ataaaaatca tgcatggctt
     4921 ggtcagccac tgctaattca ttttctttca agcctttttg tggtccgaaa acaaaagaag
     4981 cgccttcttt tccgcataaa agattagtaa catcagtaat gacagtgatt ttggctttaa
     5041 tcagctcaat aggcacgttc gttttatcaa ctgtagcaat tttacctaga ttttctccca
     5101 gtgcttctac tttttgatta tcttgatcgt aaaaagcata acctagacct tctgccatgc
     5161 cgatgccgcc atcgttggta ctacttccgc caacgccgat gatgatgtgt cgaacacctt
     5221 gttgcaacaa ttgtcgtatc agttctccta ctcctcgcgt ggataaccta agtggctgtt
     5281 gttggtttag tgggacatct ttcaatccga cgatttctgc catttcaaaa atcgcagttt
     5341 gaccgtctga agcgtactgg atttctttgt ttttgccaaa tgcattagta acgactgctg
     5401 tttgccgctt caaccttaaa ctgtctgcta atgatgaaac cgttccttct cctccatcgc
     5461 ctataggtag cagatcatat tcagcctgaa caaaaacttc ttgaaaacct gattgaatgg
     5521 cttgtgctgc ctctaaggct gtcagacttt ctttgaaaga atctggcgca atcagaatac
     5581 gcattatttt cccctccgtt ccaagatcag ttctttttcc agctggtatt ggtctccatt
     5641 gacaaaaata ggtaatgttt ctttcgtctc tttttttatt actatttgtt tttttgtcaa
     5701 aacaaatagc aacctgtgat ttttccaaat aaaaggaaaa gaaatttttt cccatgctga
     5761 aggaagtctt ggagaaatag aaagagttcc attcgttact gtaatacctg aaaaaccaaa
     5821 tataactgct agccatagtg ctcctaatga agcagcatga attccgtcat ctgatgaatg
     5881 cggatctggt cctaaatcaa tcaggcacgc ttcttggaat aaatgatacg cccattccat
     5941 ttcttggata cggcaagcct ctatggcgtg aatcgcttta ctcaacgatg agtcgtggat
     6001 tgttttgttt tcatagtaat gcaaattctt agaaacgatt ggttgagaaa aatactcagg
     6061 taataagtat aacagcatga caagatctgc ttgcttgctg acttggagtt catttacttc
     6121 gctacgggca taatcaagta aaatagactg gctgccagga cgttctttgt aagtggtcaa
     6181 atcaataact ggcttactga gatacgtgtc atcttgcgga atcagctgtt ctttgttagg
     6241 cactggcaaa tataacttat ctaaaaaatt ttgacattct ttcaaaaaaa gcggatcgcc
     6301 agcttgatat ttaacagcta aggccacatt ataatgcgcc agatagttcg tatatgcatt
     6361 attgtcgata tattctgtgt attcatctgg accaatgact ctgtgtatct ctaaatgatc
     6421 atcgactttt acagctctgc tcagccaaaa agctgctgtt tcatgaatga gcgtttttcc
     6481 ctttgtttct aaaaacgatt gatcctctgt tgcttggaca tactcttgaa cggcataagc
     6541 aaggtctgca ctgatatgat gttctgcggc agctgaagca acttcttgtc ttgttccggt
     6601 tttgatattc aatgcagcaa atttgggtgt ctcttcctga ccagtgtgtg cgctttccca
     6661 tgggaaaagt gcaccttgat agtgataatc tcttgctttt tgccgtgcgc cttctaatcg
     6721 atcataacgg tacagcagca aattctttgc tacttcagga aactgatata aaaagaaagg
     6781 caggataaaa atttccgtat cccagaaaac atgcccttta taaccttcgc cagttagtcc
     6841 cttcgcccct acactaacac ttggatcatt gcggggagtc ataatctgta aatggtaagc
     6901 agcgaagtcc atcgctaact gatcgaaaga agaatcgcct tggatcgtta tccgatgctg
     6961 atgccaaaaa tctgtccaat aagacatcga ctgctctaaa atttctgtat aactaacgct
     7021 tttctgggct tgggcaatac ttttcttaaa cgggtctgtt tgctgaagag aggtagagag
     7081 cacgatgatc ttctccagcg taaaggacgt attttttctg atcttttctg aaaaggaagc
     7141 ggtgatccgg cgattttttg cttggaatac acctgcttta ttgaatttag ctgcaatccc
     7201 aatatcaatt ttactttctg ttgtctggta ggatgcactg atcaaatcgt caccaaatac
     7261 tcgcagttct ttttcaacca actgctgtgt accaaaattg gtttgctgtc catcgatacc
     7321 cgttttcaca tctacttggc agtcttcggt caacggcttg atgaccactt tcagggctgc
     7381 ctgatggaga ttattttgat cagcaacacg acaagtaatg acttccactt tttttccttg
     7441 agcagtttca atgagcgcat agcgtttaag ctctcccatt tctaaatcta gtgcccgctc
     7501 gtatgcaaga actttcatcg tctcaagaga aaaatgttca ccgtcgattt ttatttccca
     7561 tctgatgaca tctgggagat tcactaactc ggctgattct gctcctaaag gtcggttata
     7621 tacaccagct gcaaacattc cccttacttg attccgatag tcttcctcct ggcttcctct
     7681 gataccaaaa aatccatttc caatggtcat ctgactcgca tacttattga tatactctaa
     7741 atcaaactgt ttttcttcta ctttcataag gtaataactt ctcctgtttc tgccgaacga
     7801 tataaatttt ccaccagatt ctggatgaca tatccttgcc agccatctgc aatcatctga
     7861 gtctggtcac cgagcgcccg attcacaaaa tgagccatac ttagttcatg tgggtcatta
     7921 actggcgtgg tcttttgtaa ggaaaccagc tttccatcta catcttcgaa tagttcgcct
     7981 ggatacagat tagcacctgc ctgatcgcca caaaaagcca cattcagttt gtcttctctg
     8041 atattcaaag caaacgaagt attcagttgg atgatcttgc cgttttctaa ttccagtgtg
     8101 ccaaaaattg catcttctac tgtgaatttg cttggatccc atttcccgaa tttccctgaa
     8161 ttttttttcg taccaatttt ttggaagctg tacgcccgaa cgctttttac tttaggaaat
     8221 ccagtaatat acatcgctgt atcaagcatg tgaatgccat aatcgatcaa aggcccgcct
     8281 ccttgaagtt ctttgtcgat aaacgttccc cagcctggaa caccagaccg acgaagcgca
     8341 ttcgcttcta cgtaataaat atccccgaga acatcaagat gattcttgat cattgttgct
     8401 tcgtcagaaa aacgatgctg gaatccataa gccaaaatct gcccgttttt atcggctgtc
     8461 tctgccatct ttcttgcttg atcagctgtc atagctggtg gtttctcgca catcactgaa
     8521 gcattagctt cgagggcagc caatacatgt tcgaagtgaa agcgattggc tgtacagaca
     8581 ctcacgatat cgggagaaat ctcattcagc atttgtgtaa ctgatgtata cgcatgagaa
     8641 aatcctgtac gtcggcaaaa gtcttctgct cgctggctga ctggatcaac cacagcggcc
     8701 aattctaaga catctgatct tttttgataa taagcagcat gtactttttc tgctacttgt
     8761 ccagcgccta tgattgctac tttcttcatt tatttctctc tcctttgctt atttacttat
     8821 tgttattctt tctctattct ttctctaaat catttaaatg acgcgctgtt tccagacagt
     8881 ttttcaagta ttgcaatgat tcctgataaa ttttcacttc attttctcct cgcaaacgac
     8941 attcatatac catataacct tcatactcat tttgaagtag cgtttgaaaa tggcgagtaa
     9001 aatcaattgc gccagatcct ggttggtagc gatgattatc tgctaaatgg atatgaccca
     9061 catactcttt ttgcgtagtc aatgcttttg aaatatcatc ttcttcaata ttcatatgat
     9121 aaaaatcagc gatgatctgc acatgtttca attgattaga gtcaatgtaa tgacgcgcat
     9181 ccgctaacaa attgatcata tggtcttggt atcggttcaa aggctccaaa taaataaccg
     9241 tacctgtttc ttcggctact ttatctaaat aacgaagtga ttcgctgact gctttatagt
     9301 ctccttcttt gcttcggggt gaaaccatcg gcggtaatcg ataagtgaac attccccaag
     9361 ctgctgggat aacgattcct ttgcctccaa cctcgcttag tgcacgcaaa atctcagcaa
     9421 tttgacgtac accattaaga cgccgttctt cgataaagtc accgatccac ccatcgtagc
     9481 cgttacaagc tgtcactact gggatccctg tttctttgat tgcctgtttg actgtgtcga
     9541 tattttctat taacaactta ccgtcgattt cgtattcttc aaatccacag tctttgataa
     9601 atctaaattt ttctaaaatg tctcttggga aaaatgcttg atcttgcgta ccgaatttca
     9661 ttgttctcct cctcggactt gaacgcccat tttgatactt tcttctgggt gctggtctac
     9721 ataacgcatg taagcttctg cgctttctgc aaatggcaca actggatcaa tgatttcttc
     9781 acaatccaag taaccgttca tcaataattc ccaacatgtg tcctcgattc gtttacggtt
     9841 ccatcttggg taatctgggt ttggttcact agatgctcga gcaaagacga tttttgcatt
     9901 attgaaatgt gcttcgcgac caagattaaa cccagctgga aaaggtttag caaaggcaac
     9961 ataagaaatg atcccgccat agccgatccc tcttaaagaa gcttgtaatg cggcagcact
    10021 gccactcgtt tcaataactg aatctactcc acgtttatct gtcaattcct ttagacgaaa
    10081 accgacatct gctttcatag gatctagcgt gtaatcggca ccgtattttt ctgctaattc
    10141 tctacgctgc gctatgggat cgatcccaac gatggtagta gcacctgctt ttttagctaa
    10201 ttgaatcgca atttgcccga tagcgcctaa accgaagatt gctacagcat cacctgctcg
    10261 aacatgtgct tcccggatac cacctaaagc gaattgagca ggatcgtaac atactgcatt
    10321 tttccattga tccaaccgat caagtttacg tagtttataa ttatcaacac cgtttgtaat
    10381 cagcgtttca cgaatgggtc cataagtgca gactaactct cctttttgat actctgtcac
    10441 caaactgccc gtttcttcga tcgtaccgac aatcatattt cctaattgaa attcgccaaa
    10501 taccacaccg cgttggcttc cttcttctct tggcaagaat aggcgccatt cttctgaaaa
    10561 ttcttcatcg ataaatggac ttaatcctct aaagtcgaca atttctgttc ctggttttgg
    10621 agtagcgaaa attactcgga tacgtacttc gttcttttcg atctcacggt cttcgtaatc
    10681 tttgaatccg gctattcttg gttctaatgc aactaatttt ttcatctttt ctttcctctt
    10741 tcttttcttt ttatcctttt acgccgcctt cgactaaatc acctttaata aatcgttcag
    10801 ataacgcata cataatgatg actggtaaag cggtcaccaa tgaagcggcc atcattcgtc
    10861 cccaaatgta gtctggtgta ctgaataatg tgttcaaacc gattggtaaa gtaaattttt
    10921 cggaggtcga caggaaaatc gaagcaaata aataatcatt ccacgaaacc ataaagcaat
    10981 aaacgaagac agaaatgatt ccagaaatag ccaatggtat aatgatccga ataatgatct
    11041 ggatccggct aagaccatcg atcattgctg cttcttcaat atcagtcgga atcgtgtcaa
    11101 aataactttt tagcatgaat atcgcagtag gaagtgtttg aacgaccatc gtgataatca
    11161 gtgctgtatt ggtatcatac aaacctagac cggaaatgat tttaaataat ggaacaacga
    11221 tcaaaatacc ggaaaacata taaacggtgt aaaagctagc attgattttc gagcgagctt
    11281 tgaagcgtag ttttgacaaa gcgtatgccc cgctgatccc aatcactact gcagttacgg
    11341 aggctatcaa ggagatcact aaactatttt tgaaatacat gatgaatgga aaaatctcag
    11401 cattgaaaat atcctgataa tgttcaaaag tccattcttt cggaaaaata gtttgtgtcg
    11461 ttgcaattgc ttcttgtgaa tccttgaatg atgtcatcag catgatcaaa aatggaaaca
    11521 aattgacagc taatagcaac aaaatcgtcg cgtaaaataa gattttcctg ctctttttat
    11581 ttttcatttc aagtccacct tctttcgtga gagcaagacg accgctgcaa taatgatgaa
    11641 caatagaatc gatatagctg ctgcttttcc aagatcattg aacgcaaagg ctgtctgata
    11701 caaataaacg cctaagatat tgactttatt cgtcagtaga tagacatctg taaacatata
    11761 gaacatccag atagatcgta aaacaatcac agttgctagt actggtttga ttgccggcag
    11821 tgtcacgatc ttgaacttct gccaagcatt tgcaccgtcc atttcagctg cttcataaag
    11881 cgttttgtcg actgtttgca aaatagccaa aaatgaaata aacgcatacg gatagtatct
    11941 ccagatagca aacaaaacga ccataaagaa gctagcaact ggctggtcga accacagcgg
    12001 tgcttcgttg aaaagctgca acctatctac taacagatca ttgacgattc cataactatt
    12061 attgaacata tatttccaag caaaaatcaa agaaatagat ggcgtaacat aacttaataa
    12121 aatcagtgaa cgcaccgttt tcctaaatct gaacttgcgg ttaagcaaaa tagctacggc
    12181 taatcctaaa gctgtactgc ctgctacaac taagaatgtg taagcaactg ttaaacctaa
    12241 agaacgataa aattcactat cacttaaaat ttcaagataa ttgcttaatc cgataaaaac
    12301 ggcgtctgag ttaggattca gcggcatatc gaaaaaactg atctgaatat tggaaagcat
    12361 tggaatcagc actaaaacag ctaataaaag catgcttggt gctaaaagtc ccatggctaa
    12421 ttttaagtca gaatgtttcc ttggacgatt cattgaaacc tccccctgat aatctgccaa
    12481 tagctatcta cttttttcga acaccattgg cagaacttct tgttatttcg tatttttttg
    12541 tgcgtcagct aaagcttgat cgacatcttc gtttccgaca gttacttggt tcactgcttt
    12601 tccgatcaca cctgagcttg tgatatctcc cattttcgtg aaattctttt ccccgactag
    12661 tccaaagact tgtacttcat caaaagcact agcaatctca gatggcagct cgccaaaaga
    12721 cttgatgact tcattttctt tgtaaccatc cagttcgact acttgtttat tcactggttg
    12781 agcaccacca ggagacatca agacccactt ttccatgttt ttcggttgag ataagaaagc
    12841 tgtgaatgct tcagcagcct ctttttgttt ctcgtctaga ccagaagaaa tcgtcaatcc
    12901 agatactgtg ccatagacag cttgatcttt ttgtgttggg atagcaaaac cgatattttc
    12961 tgagttcccc gcttcataaa cagaaggcaa aatgtaagtt gaatagattg ccattggtgc
    13021 tgttccattc atgaaagcat ctttgatttc tgtcacatca ttagagcctg gcatcgtgta
    13081 ttgggataag ttttgataaa acgacaatgc ttctctcatc ttttccgtat cgatcgttac
    13141 ctcgccttca tcatccaaaa catttgctcc atttgacaaa gcaaattgag agaacgcttg
    13201 ctcactcatt gttccttcag cagtaggcat agcaattcca tattgtttat tctctttatc
    13261 tgtgaaatgt tgagcaattt ttaaaatatc atcccaattt tctggttctg aaaaaccagc
    13321 ttctgataat ttttctttgt cataccaaat accttgaacc cagccgctaa ttggtgcagc
    13381 aatatagctt tttccatctt ctgaacgaac aaggtttttg gctccgtcat aataattatc
    13441 ttctccagct tcctcgataa cgttttggac tgccgtctga tcaatcagtt catctttatc
    13501 catcacttta gcaaaatcct gactgacttc cattacttct ggtaatttac cggaacgagc
    13561 taacgtaacg atttttgtat tgaacgctga ttcttctacc ggcacttgct tgattttgat
    13621 gtctggattt tctttttcaa aatcagcaac cagttcattg atcacactta atcgttcctg
    13681 ttcgacagaa gagtgcatga attgaatcgt tgtctgttgt gattcgttag agctttgact
    13741 ttcaccacaa gcagtaagaa gaaatccagt agctagcaga caggtagtaa caataatttt
    13801 tttcattttt ttccacctct tattttatta ttttcagcca catatattca tacggctgga
    13861 atatcaactt tcgttcttga ctgcaatgga taatttgtcc agaaaacagg tctaggtaat
    13921 ctccttttgg taaaagataa cctgctgatt gattggaaaa attatggata actaagactt
    13981 tctcttcctt cgcttcgcga ataaaactga taaaagaatc tgctgagtcc agtaagtaaa
    14041 tcaaactatt cggatggaag gctttttctt tttttcgaat agatagcatc tgggtcaatt
    14101 caccatatac tgctgttctt aatggatctg tctgcaactc tttctcaata tctgctaaca
    14161 aaaatttttg ccgattgatt tttcgattct ctcctgtttt ttctacgcct ttgtaatcat
    14221 ttcgacttcc cagcacactt tgaatataaa ttgccggcac gcctggtaac gttaccaaaa
    14281 atgaatgagc agctaaaaag cgtttgcttc tccattccgt cgtatctgtt ggagaactta
    14341 acgcatctag atacgtagcg tttatctcat atggactggt cgttccatct gggtttttct
    14401 tgtagtttac tcgggcaccc tctttcttca gctgttccac taattcaagg atcgtttttt
    14461 ccggcagaat acctcgtacc ggattcaatc cgataccgtc atgggaggct aaaaaattaa
    14521 aaaaagttgt ttttttcgat gcgaaatcga tggtttttaa ccattctttc aacttacttg
    14581 tattacccga acgaatcgct tgaagggtca aaggtggtag cgaaaactga tagaccatct
    14641 gtgcttcatt ctcgccattc ccaaaataac tgatattatc tttgtgtggc acatttgtct
    14701 cagtgatcaa taccgttcct ggagctgctt catcagcgat tgcacggaat aattggatga
    14761 ttttatgcgt ttctggcagg tgtatacaag aagttcctag ttctttccat aaaaatccca
    14821 cagcgtccaa gcgaataaat gaagcgcctt tttctaagta aaacaaaaga atatcgatca
    14881 ttttcaataa aacttctgga tttttaaaat tcaagtctac ttgatcttcg ctaaaagtcg
    14941 tccatacgtg tttttctttc ccgtttttgt caatgaacgt tgtaagtaac gggctggtcc
    15001 ttggtctagt cacgctgctt gtatctgtat ccagtggcaa atcaataaaa aaatctttgt
    15061 acgtatcatt caagtttaaa tattcctgaa accaagtact ttcggcagac atatgattgc
    15121 agacaaagtc gaacattaac cgtgtttcgc ttctcatttc ttctatatct tcccatgtgc
    15181 ccaaacggtc gttgattgcc tgataattta caacggagaa accatcatca gatgtatatg
    15241 ggaaaaatgg caagaaatgt acaactctaa atgtatctgc caaatacttt tgataaaatt
    15301 tcttaaatgt acgcaaagtt ggttgctgct cttcaaaaaa ttgatcccca taagtaatca
    15361 agaaaatatc ttcttcgtcc caatgcagtt tgcctgcctg gcctttcttc tgctgataaa
    15421 cactgacttt ttgttcaatc atagaggcgg ttttctcaga caaatctgag tcgcgataaa
    15481 tcgcttgtaa aagttttgta tacatcggat tcccctttct tttttgggag cgttcccaac
    15541 gatttgatca tattatggaa aacgcttact gtcaatagca tttttttaaa taattttgat
    15601 atacacaaaa atacaaataa gaaagcttat gtatcaataa ttaatagctt cttaaaaaaa
    15661 tatggtctct catgatcatg caagctattc ctaaattttc aaaatcatag acaaaaaaga
    15721 atgtctattg tactatggaa ttattctaaa acgctcccaa aaaggaggtg catataaaat
    15781 tgaaacctac tatttatgac attgcgaaaa aagctaatgt ctcaaaatca actgtctcca
    15841 gagtattaaa taaccagcca aatatctctg ataaagcaaa agcaagtgta cttcaagcaa
    15901 tcaaagaatt gaattatgaa ccgagcaaaa ttgcaagagg actgtcagga aatggttttg
    15961 acgcgattct agcattatct catcgtactt ctcatacgac ctatggcaat cctttttttt
    16021 cagatgttat tcaatccatt tctgccgttt ctgaacaaaa taacttcgac ttgattctac
    16081 aaacggcaaa aacaccagaa gaagagctag aaaaatgcgc gaaaaagatc caagaaaaaa
    16141 tgatccgagg aattattatt ttaagctcta cgatggatga aacacttctt tttgaattgg
    16201 atcaatacaa tattcctatt gtggtaatcg gaaaagtcgg aaacgactac acgaacatct
    16261 actcagtgga cacggataat ttccaggata gttacgattt ggtggaaaca ctgatcaaga
    16321 atggacaccg tcatatcggc tgtctccatg ctccgacaga agtttatgta tccaatgatc
    16381 gtctcaatgg ttaccgtcaa tgcttatttg atcaccacct agatatccgt aatgaatgga
    16441 ttatagacgg cggctttcgc ttagaagata gcatggcagc aatgaatgat ttaatgaagc
    16501 tacgagaaag accaactgcg attttttcta ccgatgcatt aaaatcattg agtatttaca
    16561 aagtggcaga acaatctgga aagcagatac ctgatgatct ttcgatcgca ggattcagtg
    16621 atcccttatt gtcctcccta ttttcgccac agttgactcg gatagacatt ccgacaaatc
    16681 agttaggaat caaagccaca gaacttttat tccaactaat ccaccaccaa taccctgaag
    16741 aaaaaagaat cattctttca accgagatta ctctcacaga ttcgatccaa caacaagaaa
    16801 tgtagaacag aattataaaa gaacagaaac actgaggata agtcagttac tctgttcttt
    16861 tttcaatcac tcatccctcg ttaccccctc ccggtatttt agcgaatttt tcttattttc
    16921 catctgtttt agatagctgt ggataactat aatgtttgtg ttaaactatt ttttccacag
    16981 ggaagaaaaa actattttta accacgttgt gtataagttt ttatagttcg tttttttaaa
    17041 cacatttacc cttatttcaa tccatgttat ttggggatac ttatctctaa aacataaata
    17101 gaataacaga catgtctact ggttttgttt tattcgagga attattcaat aatagctagc
    17161 aaaatattaa aaataaccgc tctatcttcc catcaattta ttttgcattc aaaaaatgat
    17221 ctatctttac ttttgttgat tgacttctct tggaagatga tcgttccgat accttaattg
    17281 gatacgaacc gatgttgtta agatcctttc ccacttttct tctagttaga caaattattt
    17341 tagacaaacc aaaaaagagg ttgtgacaat aatttgtcac aacctctttt ttaaaatatc
    17401 tattttgttt gattgttcat tgcgtcttct gcagccattt gcgcttcaat atctgaaata
    17461 ctgtagacat tttcgcttgc tacaccgact tcttttggtt ccatgtcacg ataacgttgc
    17521 ataccagtac cagctgggat gatcttacct ataataacat tttctttcaa accaagaaga
    17581 ggatctttct taccgcggat cgcagcatcc gtcaagacac gagttgtttc ttggaatgat
    17641 gcagcagata agaaactatt tgtttctaat gatgcttttg tgatacctag taagacaggt
    17701 cgagaagttg caggaacgcc tcctgagact agcgtattgt agttttgatc tttaaagtct
    17761 gcgatgtcta ataatgtacc tggtaagata tctgtatcac ctggatccat tacacgaact
    17821 ttacgcagca tttgacgaac cattacttcg atatgcttgt cgccgatttc taccccttgc
    17881 atacggtaaa cacgttgtac ttcacgaagc aagtagtttt caacagataa tacatcgcga
    17941 acttgcaaca attgttttgg atcgattgat ccttctgtca atggtgcacc acggtggatc
    18001 atatcgcctt ctgctacttt catacgtgct gtataaggaa cagtatacgt acgtgtatct
    18061 gtttttcctt tgattgttac ttctttggta cgtgttgcag gatcttctga gatatcgatg
    18121 acatcccctg ttacttctgt gatcactgct tgccctttcg gattacgtgc ttcaaagatt
    18181 tcttggacac gaggaagacc ttgagtgata tcgtctccgg caaccccacc tgtatggaat
    18241 gtacgcatcg tcaactgagt accaggttca ccgattgatt gagcggcgat cgtaccaact
    18301 gcttctccga cttcaacgtc agagccagtt gcaaggttgc gtccgtaaca atgtttgcag
    18361 acaccatgtt ttgtattaca tgtgaagaca gaacggattg tcactgtttc gacacctgca
    18421 tcaacgattt gttttgcgat gtcttcggaa atcaattggt ttcctgcaat gatcaattca
    18481 tttgtttcag gatgacgtac ttctttacgg gtgtaacgtc ccactaaacg atcttctaat
    18541 gattcgatga tttcatttcc ctcgcggatg gcttggattt caagacctcg gtcagtaccg
    18601 caatcttctt cacggatgat tacatcttgg gccacgtcaa ccagacgacg agtcaagtaa
    18661 cctgaatcgg cagtcttcaa ggctgtatcg gtcatccctt tacgagcccc gtgagttgag
    18721 ataaacattt ccaagacgga tagtccttca cggaagttcg aaatgattgg caattccatg
    18781 attcgaccat taggagcggc catcaaacca cgcattccgg caagctgtgt aaagttggaa
    18841 atgttaccac gagctccaga atcagacatc atgaagattg gatttttcgc ttccaaacct
    18901 tccatcagtt tttgttggat gctgtctttt gcagcattcc aaacagcaat gacacgttca
    18961 taacgttcgt catctgtgat cagaccacga cggaattgtt ttgtaatagt ttcaacttgt
    19021 ttatgggcat tttcaatgat ttcttgtttt tcattcagta cactgatgtc agcgatccct
    19081 actgtgatac cggcatgtgt cgaatgtttg taccctaagt ctttcatacg gtcaagcatc
    19141 ttagaagttt cttttacttt gaatcgtttg aagacttctg cgatgatgtt tcctaagttt
    19201 ttcttcttga acggtaatac taattcttgc tctttgatat gtgcaggaat atcagtacca
    19261 gcttcaacaa agtatttgtc cggtgtttgg acggtcaagt tgaagtctgt tggttcattc
    19321 agataaggga attcaggagg catgatctcg ttgaagatga tcttaccaac tgttgtgatc
    19381 atcgtacgtt ctttttgcca ttcagtaaat ggtttttcac ctaatgagtt agggttcaca
    19441 ccgatacgtg aatgtaaatg aacatatccg ttgcgccatg cgataacggc ttcgttttga
    19501 tctctgaaga ccattccttc gccttcgcgt ccttcttctt ccattgtcaa gtagtagtta
    19561 cctagaacca tatcttgaga aggagtaaca actggtttac catctttagg gttcaagatg
    19621 ttttgagcag ctaacatcaa catacgtgct tctgcttgtg cttcttcatt caatggaacg
    19681 tggacagcca tttggtctcc atcgaaatcg gcattataag cttcacatac aagtggatgc
    19741 aaacggattg cgcgtccttg tactaaaact ggttcgaatg cttggatacc aagtcggtga
    19801 agcgtaggtg cccggttcag caatactggg tgttccttga ttacgtcttc taaaacatcc
    19861 catacttcgt cttcttgacg ttcgatttta cgtttcgcat ttttgatgtt tgaagctagt
    19921 tcgcgttgaa caagctcacg catcacgaat ggtttgaata attcaatggc catttctttt
    19981 ggaagtccac attggtacat cttcaagaaa ggtccaacaa cgataacgga acgacctgag
    20041 tagtcgacac gtttacctag caagttttga cggaaacgac cttgtttccc tttcaacata
    20101 tgagaaagag atttcaatgg gcggttacct ggtccagtaa ctggacgtcc gcgacgaccg
    20161 ttatcgatca atgcatctac tgcttcttgc agcatacgtt tttcattttg aacgatgatt
    20221 ccaggtgcat tcaagtctaa caaacgtttc aaacggttat tacggttgat tacacgacgg
    20281 taaagatcat tcaagtcact tgttgcaaaa cgtccacctt ctaattgaac cattgggcgt
    20341 aaatctggtg gaataacagg gataacgtcc ataaccatcc agcttggttg atttcctgaa
    20401 gcgcggaatg cttctaaaat atccaaacgg cggattgcac gtgtacgttt ttgtccttgt
    20461 gcagatttca attcttcttt caattcggct gcttctgcat ctagatcaac attatcaagt
    20521 aattgtttaa tcgcttctgc acccatagca gcttggaatt cttgtccata ttgttcacgt
    20581 ttttcgcgat attcgcgttc tgtcaacaat tgttttttct ctaaattcgt atttcctggt
    20641 tcgatgacta catatgaagc aaaataaatg atttcttcca aagcgcgtgg gctcatgtct
    20701 aatacaagac ccatacgtga aggaatccct ttaaagtacc agatatgtga aacaggagct
    20761 gctaattcaa tatgtcccat gcgttcacgg cgaactttcg aacgagtaac ttccactccg
    20821 catcgatcac aaacgattcc tttataacgg atacgtttgt attttccgca ggcacattcc
    20881 cagtcttttg taggaccaaa aatccgctca cagaacaacc cttcgcgttc aggttttaag
    20941 gtacgatagt taatcgtctc aggttttttt acttcaccgt aagaccagct tctgattttt
    21001 tcgggagaag ccaaacctat ttgcatactt tcgaatttat ttacatcgat caaaaggggt
    21061 tccctccatt tcatttaatg gcaaaccgca agacggctga tcttcgaccg ccttgcagct
    21121 catagaccgg attagtctaa attatcttct gcggtttcaa agttttggat gacatcgtct
    21181 ttttcatctt ccacttgttc agcagctttc ttttctaatt ctttggcagt ttgctgttcg
    21241 gcaaattttg tcaaagcatc aacagtgatc aaatcgtcgt cttcatcgtc catgtcgcga
    21301 agttcgatct ctgtttcttc gatatccaag acacgcatgt ccagacctag tgattgcaat
    21361 tctttcacca atacgcggaa tgattccggt acacctggtt ttggaattgg ttcacccttg
    21421 acgattgctt cgtatgtttt cacacgtccg actacgtcat ctgatttgta tgtcaagatt
    21481 tcttgtaaag tataagccgc accgtaagct tccagtgccc atacttccat ttcaccaaaa
    21541 cgttgtccac caaattgagc tttacctccc aatggttgtt gtgtaaccaa tgagtaaggt
    21601 ccgattgaac gtgcatgcaa tttgtcatcg accatgtgag caagtttgat catgtacatc
    21661 acaccgacag acacacgtcc atcaaatggt tcacctgtac gtccgtcgta cagaacagtt
    21721 ttcgcatcgc tggccatacc ggcttcacga actgtttccc atacgtcttc atcacttgca
    21781 ccatcaaata ctggcgttgc tacgtggata cctaactgac gtgcagccat acccaagtgt
    21841 aattccaata cttgcccgat gttcatacgt gaaggcaccc ctaaaggatt cagcatgata
    21901 tcgattggtg ttccatcagg taggaatggc atatcttctt ccggcataat acgggataca
    21961 acccctttat ttccgtgacg accggccatt ttatcccctt catggatttt acgtttttga
    22021 acgatataaa cacgaactaa catgtttacg cctggtgaca attcatctcc agcttcacga
    22081 gtaaagattt tcacatcgtg aacgataccg ccgccgccat gtggtacacg taatgatgta
    22141 tcgcggactt cacgagcttt ttcgccaaag atcgcatgca atagacgttc ttctgcagat
    22201 aattctgtta ccccttttgg cgtgactttc cctacaagca aatcgccatc ttggacttct
    22261 gcaccaatac ggatgatacc catttcgtct aaatctttta atgcgtcttc cccgacatta
    22321 gggatttcgc gtgtgatttc ttcaggtcct aattttgtat cgcgggcttc tgattcatat
    22381 tcttcaatat ggatagaagt gtatacgtca tcttttacca aacgacggct catgatgatc
    22441 gcatcttcat agttgtaccc ttcccaagtc atgaaaccaa ctaaaacgtt ttggcctaga
    22501 gccatttcgc cttgttccat agatggtccg tctgccagag tatccccttt ttcgactttc
    22561 tcacccaaat gaacgattgg acgttggttg tagcttgttc cggagtttga acgacggaat
    22621 tttgttacat cgtatttatc taatgcaccg ttgtcgcggc gtacgcggat ttcagaagca
    22681 tcaacgtatt caacgacacc atcatgttta cataacaaag cagctcctga gtcatgcgct
    22741 gatttgtatt ccataccagt accaacccat ggtgcttgag gattgatcaa cggcactgct
    22801 tgacgctgca tgttggcacc catcaaggca cggttggagt catcgttttc caagaaagga
    22861 atacatgctg tcgcaacggc aactacttgt ttaggtgata cgtccatgta gtctacttta
    22921 tcaatagata cttctaagtt ttcactttga gcacgagcca taacaacgtc ttgagcaaat
    22981 gttccatctt cattcaatgg gctgtttgct tgggctacga tgtaatggtc ttcgatatca
    23041 gctgtcaagt agtcgatttg gtcagtaaca cgacctgttt cacggtctac acgacgataa
    23101 ggtgtttcga taaatccgaa tttatttact ttcgcataac tggacaaact attgatcaac
    23161 ccgatgtttg ggccttcagg agtttcgatc ggacacatac ggccatagtg ggagtagtga
    23221 acgtcacgaa cttcataacc ggcgcggtca cgagtcaatc cgccaggtcc taaggcgaaa
    23281 agacgacgtt tgtgtgtcaa ttcgccaaga gggtttgttt gatccatgaa ctgtgacaac
    23341 tgagaagaac caaagaattc tttgatactt gccacaactg gacggatatt gatcaattgt
    23401 tgaggtgtca atgtttcagt atcttggatc gacattcttt cacgaaccac acgttccata
    23461 cgtgctagcc cgatacggaa ttggttttgt aaaagttcac cgactgaacg gatacgacga
    23521 tttcctaagt ggtcgatatc atcgacattg ccgatacctt ccatcaagtt caagaaatag
    23581 ctcattgaag caatgatgtc ggctggacga accgttttta cagctgcttc tggatagccg
    23641 tttccgatca cgtttacttc acgttcaggg tctcttggtg aaaatacttt gatgacttgg
    23701 acagtcattg gatctgtgac cacaccgtct tctgatggat agtaagtcac tgaatttagg
    23761 ccattatcaa tgaaaggagc tagtgtttcc atgacttggt gagtcagaac tgttcctttt
    23821 tcaacgatga tctcgcctgt ttctggatca actaatgttt cagctaatgt cagattcaat
    23881 agacgtgttt tcaaatctaa ttttttgttt actttgtaac gaccaacgtt tgctaagtcg
    23941 taacgttttg gatcgaagaa acgagcattc agcaagttac gtgagctgtc tgctgttttt
    24001 ggttcgcctg gacgcaaacg ttcgtaaacg tctttcaaac cttcttctgt tcttgagtcg
    24061 cttgcgtttt tatgcaagtc tttttcgact gtattgcgta atgtttcgct gtcgccgaag
    24121 atttcaaaga tcgtatcatc ggaaccaaat cctaacgcac gaactaatac ggttaaagga
    24181 atcttacgtg tacggtcgat acgtacataa gagatgtctt ttgcatcagt ttccatttct
    24241 agccaagctc cacggttagg gataacagta gaaccgaatc cttcttttcc gtttttgtcg
    24301 acttttccat ggaaatagac acctggtgaa cggactaact gagaaacgat tactcgctct
    24361 gccccgttga tgatgaatgt tccttgttct gtcattaatg ggaaatcacc gaagaatact
    24421 tcttgtgctt tgatttcgcc agtctcgcgg tttgtcaaac gcaatgttac atgtaatggt
    24481 gcagagtagt ttgcatcatg cgcacgagct tctgctactg tatactttgg ttctttcaat
    24541 tcataatcta caaattccaa agatagattg ccattgaagt catcgattgg caaaatgtct
    24601 tcaaacattt ctctaagtcc ctcatctaag aaccattggt aagagtctgt ttgaatttcg
    24661 atcaaatttg gtaattccaa cacttcactg attcttgcga aacttctacg ttcgcgatgt
    24721 tttccgtatt ttactacgtg tccagccaag ctcttcaccc ctctaaaatg tcattcaaaa
    24781 agactacccg atcaattgtt acaataatat ttcaaatatt atttttaggt aacaacagac
    24841 attttttgcg gcttttaggc aaaaaaaaac caaaaataga acattctctt gaaaccttac
    24901 ttcgtgagct tctacttttg tttccttata ttaaagccga aacggacaat atttcgtttc
    24961 tgccttttct tcatcagata gtttttgcaa cttcatatac tacacttatt ttctataaaa
    25021 gtcaacactt cctggcgttt cttctgcttt ttcgcaacat tttcttaatg ttttcaattt
    25081 tatgttttca attatctgac aattcttaat tgacagacct ttttatctcg gttatacttt
    25141 agaaaattca ttttgtggag gaacttctat gcgaaccaac atcaaccaaa tcgcaccaac
    25201 acaactgaat aacttttact ttagctactt tagataggtt tgtattcatc atgatggata
    25261 caaacgttaa acgacagttt gtatctatga tggctaaaga actttgtatt gcagcaaaaa
    25321 gccacgtaga caaaatgcta agtggctgag cagtgaaaat acaaagtaaa agtatcaagc
    25381 attctacttt ttcaatttat tttccgactt ttcaatccat ttggacattt tgtctgaatg
    25441 gattttttgt ttcccaaaat taaggaggaa aaagaaatga aaaaaaatat aatcatcgga
    25501 ttaggcgctg ccctgctact agctttagga ggttgcgcac aaaaagacaa ttccgcagag
    25561 gcatcagatg gacaaaaaac aattggcgta ttgcaacttg tacaacataa ctcgttagat
    25621 gctgcctata aaggattcaa agaaggatta gcagaagaag ggtataaaga aggcgataat
    25681 ctaacaatcg attatcaaaa tgcccaaaac aatcaagaca acctgaaaag tatgagtgag
    25741 aaactcgtca aagaagatcc tgatttgcta cttggaatcg ccactccagc agcacaatct
    25801 ttattaaatg aaacaaccac tatcccgatg actgtcacag cggtcacaga cttggaacaa
    25861 gcccatttag tcacatccaa tgaaaaacct ggcggcaatg tgactggcac gacagatatg
    25921 gtgccaatcg aaaaacaaat cggcttactg ttaaatatcg tgccagacgc caaaacaatc
    25981 ggcatcatgt ataacaacgg tgaagcaaac tctaagatcc aagctgattt ggcagaaaaa
    26041 gaattgaaaa aagcgggcgt caaagtcaaa gtactgacag caaattccac aaatgatgtc
    26101 caacaagtga cgaaaagtct agctaaagat gtagatggaa tctatattcc tactgataac
    26161 acgttcgcaa gtgcggcaag tgttgtcggt gaagtcgcaa aagaaacaaa aacacctgtc
    26221 gttgctggcg cagttgatca agtcaaagac ggcggtctag caacagtagg aatcgactac
    26281 gaaagcttgg gcaaacaaac agggatcatg gcagcaaaaa tcttagacgg caagaatcca
    26341 agtgaacttc ctgtcgaaac agcgaatgat ttggaactgt atgtcaataa agacatggcc
    26401 aaagcattag gcatcaatcc agacagcatc aaagaagctg aataaaacac aggaggattc
    26461 tcatgacaaa tttgctctct cttttcttat catctgcatc gcaaggggtc ttatgggccc
    26521 tacttgcgat tggtgtatac gtcactttcc gtatcttgga cattgccgac ttgactgctg
    26581 aaggaagctt tcctttaggt gcagggatcg cggcagtaag tattacaaat ggctatcatc
    26641 cattgacagc ttgtcttcta ggctttttag ggagtgcggc agcagggctt gtttccggac
    26701 tgcttcacac aaaattgaaa attcccgccc ttcttgcagg gatcatcacc atgactggtt
    26761 tatattctgt cacatcccgt gtgatgggtg cgccgaacat ctctttattg ggtcagccaa
    26821 cgatctttca ttgggtcgag tcattcggca taacaaaaga caacgctgta ctgatcgtcg
    26881 gtctgctttt tgctttgatc gttgtatgtt tacttgtttt gttcttacgt acagaaacag
    26941 gacttgctat tcgtgcaact ggcgataatc tggcaatgag cgaagcaaac gggatcaaca
    27001 cagataatat gaagatcatc ggttatatga tttctaatgg atgcattgct ctttctggct
    27061 ccttgcttgc gcaaaacaac ggctttgctg atttaaattc tggtattgga acgatcgtaa
    27121 ttggacttgc ttctattatc atcgcagaag ttttgttacg taaccagcca ctatttctcc
    27181 gtctcttgac tgtcgtttta ggtgcggtca tttatcgttt tattttagcg ttagtcttcg
    27241 aactgaatgt tgaaccaagt gattcgaaac ttgcttctgc tttcgtgcta gtgatctgtc
    27301 tttctttccc gcagatccag caaaaattcc atttacctca aaaaaagaaa ggcggtagtg
    27361 tcaaatgaat gacgtcttaa cattggaaaa tctccatcat acctttgaaa aaggaacgat
    27421 caacgaaaat catgtcctga aaggcattaa tctttcctta gatcccggcg acttcgtgac
    27481 gatcatcggt ggaaatgggg ctggtaaatc tactttgctg aacagcatcg ccggtacatt
    27541 ttctgtcgat cagggtcaaa ttttattgaa tggaaaaaat atcacgaaaa aatctgttgt
    27601 cgaacgttcg aaatccatca gtcgcgtatt ccaagatcct aaactaggga cagccgttcg
    27661 tttaacggta gaagaaaatc ttgctcttgc tatgaaacga ggaaaaaaac gcggcttctt
    27721 ccgtggtgtc aaaccacaag accgaacatt tttcaaagac cagctggttc ggctaaattt
    27781 ggggttggaa aatcgtttat cctcagaaat cggtttgctg tctggaggac aacgtcaagc
    27841 tattacgtta ttgatggcca cattacagcg tccagaactg atcttattag atgaacacac
    27901 tgcagcgctt gaccctgcta cttccaaaac ggtgatggaa ttgacagaaa agctgatcac
    27961 tgagcagaat ctcactgctt ttatggtcac tcatgacatg gaagacgcga tccgctatgg
    28021 gagtcgactg atcatgctgc atcaaggaag gatcgtcgta gatatccagc aagaggaaaa
    28081 acagcagctc acagtgcccc aattattagc gttgttcaaa caaaacagcg gcacggcatt
    28141 aaaagacgat caagtacttt tatcttcata atcagaaaga ggatgggaca aaaaatgaac
    28201 gcccaccctc ttcttttttc tctttccttc tttcgttttt gctttatgta tcaaccgttg
    28261 tttcttcgaa aactattttg ttctctcctt cttcctgata gaatggatgg gaaaggagct
    28321 tgatgccatg acaacgaaag acaaggtact tcaattattg aaaaagacac cagattttct
    28381 ttccggagaa aagttggcac agcaactgga agtttcccgg acaagtattt ggaaagccat
    28441 caaagaattg gaaaaggcag gctatcgttt tgagcacggg cctacagggt atcgttacct
    28501 gccctctgac gtactagatg caaaagaaat ccagcaagag ctacggtcgt ttattcctga
    28561 tttgaaagta caaacgatga tgacttctga atctacgatg aaagatgcca aactggcagc
    28621 agctcaagga gattctgttt cgaagttatt tgttgctgat acacaagaac aagctcgtgg
    28681 acgttttgga cggccttttt tcgctgaacc tggacgagga atctacatga gtctcttgct
    28741 ccatccagac aaacggtttg atgaattgcc tcaatatacg atcatcgcag ccagtgcctg
    28801 tgtgaaagcc attgaatctg taaccggtcg ttctgcttcg atcaaatggg tcaatgatat
    28861 ctacatggat ggaaaaaaag tctgtgggat tttatctgaa gcagtcagtg atatggaaag
    28921 cggcaggatc agtcatatca tcattgggat cgggcttaat ttttctgttc ctcaaaatca
    28981 ttttccagct gatctccaag aaaaagccat ctctgtcttt cccgacggac aagctagtgt
    29041 cacaaggaat cagctaatca gcaaaatctg gcaagaattg ttcacttatc tttctcaccc
    29101 agaaaaagcg ttggacttct ataaagaaaa atcattcgta ttgggaaaaa ccgtgtcgtt
    29161 caatcaaaaa ggaacgactt atgtcggtct tgcaaaagaa atcggtgatc atggcgaact
    29221 gatcgtccaa acaaatgaac agaagatgag tttgtcttct ggcgaaatca gtctcacatc
    29281 tatccgtacg caataattat cagatttcac aaaaggacac ctgatctcat acagtgtcct
    29341 tttttctgaa ttctcttcaa aagactctcc ttattctatc agctgacagt ttctctatcc
    29401 tctagaaaaa ggtattctaa aaatatctat aaagcatggt acactttctt taaatgaatc
    29461 aatgcaaaaa acaagagagt catcagttta cacgatcaaa agagatagca ggccgcttcc
    29521 tttttcaatc ttatagatag accatgggta acgataaaaa caaaaaagaa cttgcgactt
    29581 attttgccgt aagttctttt ttagctagtg ccttaatctc ttaatcccaa caaacgtctc
    29641 gcatagatca cctgaccaga gtgcatagca gcatcatcaa taatagaaac gagacgcatt
    29701 cctcgtgtga ctggtggtgt ccagttctcg tcaattactt catctagctc ggaaggttcc
    29761 agccttctta agtaagaaat agcaaaatca gcagctcctc gcaagtatcc tagcagatct
    29821 tctatttgat tagcttggat ctgctttgct tgttccagac tatgcttcca atcttgttcg
    29881 ttatctgcta tcgtcatcgg aaaccggctt tcccagtctt gactatgcca aattggagaa
    29941 gttcctgcta aatcagcaat ctggaaatct aattctcgag cggtatgcca agccagccaa
    30001 gtcattgatt tgatctgcgg ggctctatct tccactggaa aacgattggc ctcctcaata
    30061 gagacgcctt caaacaaacg ttcaaaccga tcgtatgccc gatccacact ctcgcatagc
    30121 atatctaaat aatccattta ttttcctcct ttttatttat cctacttact ttttcctaat
    30181 tctaggatac ctacttatat gttactacac acccttattt tttacacttt aacagtcacc
    30241 atcatcaata gtttggagat tgaaagaaaa agatagtgtc gaactatcaa ttgaacaaga
    30301 agcaacaata tgaacgtttc attaatattc tttaaataaa caatgatttt tcaaacgaac
    30361 agtttaagac taggagaaaa agagtataat aggaaaatgt ttgaaaggaa aggaagggga
    30421 aaaatgaaaa aaagggcaag tttactagtc ctagttgctt tgttgttcaa tatcttttcg
    30481 tatggtgtgg ccgtttcagc ggaaacatac attcctaaag accctgggaa tatttataat
    30541 gatctaactg gtaactatgg cttgttagga attgctagtc aattccacgt atttgctaaa
    30601 aataagacaa ctgtgaatgc acatacagac ggaaatatag caacacatga acttgatgca
    30661 aaaaataatt ttggtacaga gcttcatgaa ggtttattga accaagaaat aaattatata
    30721 caaaaaactg attctttcgc taattcttca ggcataccta caggtagcac aagaatgaat
    30781 aaatttgtcg ttggcagtaa cgtggatgta gcacttcaag atggtcatcc tacagtcaat
    30841 ggatccaggc ttgatcactt aactccagat gatttttata aagatcgtgc tgggcatgaa
    30901 tatattgatt tcgagcagga atttgctaaa ttgaatgtag cagcaaatga tcttgcaacg
    30961 atcactccag ctaaaacata tactgctgca gattttccag acatgaacaa tcggacaatc
    31021 gatttaactg atttatcaga taccgggttt ttattggtaa atatcgatgc agaagtgtta
    31081 acgatgaata cgccacttca aatcattaat ccaaatgatc aagtagttgt atttaatgtc
    31141 atcaacagtt cttctgcatt gaacgtacag tcaccaatca aatacaatga tcgttcaaat
    31201 catgaaacag aagatttttc ggatgcgaat atctcttgga attttggaaa tgagatgact
    31261 gatttgacaa tcagtgctcc ttttcaagga acaatccttg cgcctaatgc tactatccgc
    31321 attactcaaa accaagacgg aacgattata ggaacaaatg tgattttaga tgcagctacc
    31381 aatcgttggg atcctaacga aatatttatc agcgacaatg gtacagatac gacagacaca
    31441 agcgacagta cagatacatc taccacaagt gatagctcag attcatca
//
LOCUS       DACJGB010000028        31410 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.028, whole genome shotgun sequence.
ACCESSION   DACJGB010000028 DACJGB010000000
VERSION     DACJGB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.028"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            261..1130
                     /locus_tag="IYI34_001337"
     CDS             261..1130
                     /locus_tag="IYI34_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAR0559213.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1339..2364
                     /locus_tag="IYI34_001338"
     CDS             1339..2364
                     /locus_tag="IYI34_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAR0559214.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2403..3353
                     /gene="manA"
                     /locus_tag="IYI34_001339"
     CDS             2403..3353
                     /gene="manA"
                     /locus_tag="IYI34_001339"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAR0559215.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3447..5150
                     /locus_tag="IYI34_001340"
     CDS             3447..5150
                     /locus_tag="IYI34_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0559216.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5152..7185
                     /locus_tag="IYI34_001341"
     CDS             5152..7185
                     /locus_tag="IYI34_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR0559217.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7196..7636
                     /locus_tag="IYI34_001342"
     CDS             7196..7636
                     /locus_tag="IYI34_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR0559218.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7836..8963)
                     /locus_tag="IYI34_001343"
     CDS             complement(7836..8963)
                     /locus_tag="IYI34_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559219.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8966..9643)
                     /locus_tag="IYI34_001344"
     CDS             complement(8966..9643)
                     /locus_tag="IYI34_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559220.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9907..11673
                     /locus_tag="IYI34_001345"
     CDS             9907..11673
                     /locus_tag="IYI34_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAR0559221.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11688..12125
                     /locus_tag="IYI34_001346"
     CDS             11688..12125
                     /locus_tag="IYI34_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR0559222.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12138..13289
                     /locus_tag="IYI34_001347"
     CDS             12138..13289
                     /locus_tag="IYI34_001347"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAR0559223.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13351..14049)
                     /locus_tag="IYI34_001348"
     CDS             complement(13351..14049)
                     /locus_tag="IYI34_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAR0559224.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14042..15394)
                     /locus_tag="IYI34_001349"
     CDS             complement(14042..15394)
                     /locus_tag="IYI34_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAR0559225.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15617..17017)
                     /locus_tag="IYI34_001350"
     CDS             complement(15617..17017)
                     /locus_tag="IYI34_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAR0559226.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17178..17915
                     /locus_tag="IYI34_001351"
     CDS             17178..17915
                     /locus_tag="IYI34_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAR0559227.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18040..18519
                     /locus_tag="IYI34_001352"
     CDS             18040..18519
                     /locus_tag="IYI34_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAR0559228.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18516..19283
                     /locus_tag="IYI34_001353"
     CDS             18516..19283
                     /locus_tag="IYI34_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559229.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19513..20082
                     /gene="lepB"
                     /locus_tag="IYI34_001354"
     CDS             19513..20082
                     /gene="lepB"
                     /locus_tag="IYI34_001354"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAR0559230.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20186..20788)
                     /locus_tag="IYI34_001355"
     CDS             complement(20186..20788)
                     /locus_tag="IYI34_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAR0559231.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20894..21363
                     /locus_tag="IYI34_001356"
                     /pseudo
     CDS             20894..21363
                     /locus_tag="IYI34_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21469..22053
                     /locus_tag="IYI34_001357"
     CDS             21469..22053
                     /locus_tag="IYI34_001357"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAR0559232.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22056..23129
                     /gene="prfA"
                     /locus_tag="IYI34_001358"
     CDS             22056..23129
                     /gene="prfA"
                     /locus_tag="IYI34_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAR0559233.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23122..23961
                     /gene="prmC"
                     /locus_tag="IYI34_001359"
     CDS             23122..23961
                     /gene="prmC"
                     /locus_tag="IYI34_001359"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAR0559234.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23954..24133
                     /locus_tag="IYI34_001360"
     CDS             23954..24133
                     /locus_tag="IYI34_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559235.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24155..25180
                     /locus_tag="IYI34_001361"
     CDS             24155..25180
                     /locus_tag="IYI34_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAR0559236.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25371..26615
                     /locus_tag="IYI34_001362"
     CDS             25371..26615
                     /locus_tag="IYI34_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAR0559237.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26768..27715
                     /locus_tag="IYI34_001363"
     CDS             26768..27715
                     /locus_tag="IYI34_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR0559238.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27849..28478
                     /gene="upp"
                     /locus_tag="IYI34_001364"
     CDS             27849..28478
                     /gene="upp"
                     /locus_tag="IYI34_001364"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAR0559239.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28617..28859)
                     /locus_tag="IYI34_001365"
     CDS             complement(28617..28859)
                     /locus_tag="IYI34_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559240.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28980..29052
                     /locus_tag="IYI34_001366"
     tRNA            28980..29052
                     /locus_tag="IYI34_001366"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29013..29015,aa:Thr,seq:cgt)
     gene            complement(29171..29809)
                     /locus_tag="IYI34_001367"
     CDS             complement(29171..29809)
                     /locus_tag="IYI34_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAR0559241.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29932..30564)
                     /locus_tag="IYI34_001368"
     CDS             complement(29932..30564)
                     /locus_tag="IYI34_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAR0559242.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30915..31214
                     /locus_tag="IYI34_001369"
     CDS             30915..31214
                     /locus_tag="IYI34_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAR0559243.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10721 a         5478 c         6317 g         8894 t
ORIGIN      
        1 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat
       61 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
      121 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
      181 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      241 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      301 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      361 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      421 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      481 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      541 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      601 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      661 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      721 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      781 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      841 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      901 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      961 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
     1021 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
     1081 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1141 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1201 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1261 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1321 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1381 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1441 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1501 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1561 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1621 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1681 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1741 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1801 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1861 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1921 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1981 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     2041 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     2101 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2161 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2221 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2281 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2341 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2401 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2461 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2521 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2581 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2641 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2701 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2761 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2821 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2881 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2941 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     3001 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     3061 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3121 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3181 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3241 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3301 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3361 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3421 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3481 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3541 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3601 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3661 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3721 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3781 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3841 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3901 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3961 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     4021 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     4081 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4141 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4201 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4261 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4321 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4381 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4441 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4501 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4561 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4621 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4681 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4741 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4801 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4861 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4921 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4981 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     5041 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     5101 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5161 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5221 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5281 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5341 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5401 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5461 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5521 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5581 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5641 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5701 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5761 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5821 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5881 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5941 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     6001 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     6061 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6121 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6181 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6241 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6301 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6361 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6421 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6481 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6541 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6601 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6661 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6721 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6781 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6841 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6901 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6961 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     7021 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     7081 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7141 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7201 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7261 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7321 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7381 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7441 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7501 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7561 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7621 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7681 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7741 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7801 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7861 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7921 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7981 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     8041 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     8101 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8161 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8221 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8281 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8341 aaacggatgc tatgtttctt taccatacta tccagtaata gattgactag agagattacc
     8401 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8461 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8521 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8581 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8641 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8701 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8761 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8821 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8881 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8941 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     9001 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     9061 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9121 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9181 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9241 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9301 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9361 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9421 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9481 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9541 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9601 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9661 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9721 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9781 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9841 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9901 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9961 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
    10021 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
    10081 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10141 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10201 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10261 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10321 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10381 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10441 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10501 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10561 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10621 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10681 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10741 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10801 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10861 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10921 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10981 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    11041 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    11101 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11161 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11221 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11281 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11341 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11401 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11461 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11521 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11581 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11641 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11701 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11761 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11821 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11881 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11941 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    12001 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    12061 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12121 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12181 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12241 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12301 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12361 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12421 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12481 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12541 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12601 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12661 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12721 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12781 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12841 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12901 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12961 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    13021 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    13081 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13141 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13201 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13261 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13321 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13381 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13441 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13501 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13561 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13621 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13681 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13741 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13801 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13861 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13921 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13981 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    14041 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    14101 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14161 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14221 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14281 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14341 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14401 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14461 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14521 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14581 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14641 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14701 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14761 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14821 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14881 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14941 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    15001 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    15061 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15121 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15181 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15241 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15301 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15361 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15421 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15481 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15541 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15601 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15661 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15721 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15781 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15841 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15901 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15961 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    16021 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    16081 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16141 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16201 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16261 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16321 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16381 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16441 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16501 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16561 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16621 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16681 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16741 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16801 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16861 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16921 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16981 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    17041 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    17101 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17161 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17221 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17281 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17341 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17401 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17461 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17521 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17581 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17641 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17701 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17761 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17821 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17881 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17941 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    18001 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    18061 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18121 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18181 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18241 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18301 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18361 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18421 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18481 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18541 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18601 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18661 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18721 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18781 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18841 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18901 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18961 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    19021 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    19081 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19141 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19201 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19261 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19321 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19381 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19441 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19501 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19561 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19621 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19681 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19741 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19801 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19861 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19921 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19981 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    20041 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    20101 ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat ttcttggagt
    20161 tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag aaaagcgaag
    20221 cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta gcagagacaa
    20281 agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta tggactttcg
    20341 gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg ccaggagcta
    20401 aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa agtacaagtg
    20461 atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca gcgtggatcg
    20521 ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc agcatagttt
    20581 ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa ctaacaagcc
    20641 cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt agataagtag
    20701 ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca taatggttag
    20761 cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac tttgaaattt
    20821 taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca aaaagggaga
    20881 atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag gaaagtgtgt
    20941 ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa cggaaaaata
    21001 tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag aatatttagg
    21061 ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta ttgtatggga
    21121 ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga agcagaaggt
    21181 tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg gcactactct
    21241 atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt ggaagttttt
    21301 ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt ggaacaaaag
    21361 tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta aaaaatgctg
    21421 aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat ggcacaacta
    21481 ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa agtcgcacat
    21541 aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga tacaagagat
    21601 ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc gatatttgaa
    21661 gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg tgtgctgatc
    21721 gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat tgtggacgaa
    21781 ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga gttatttgaa
    21841 ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac aatctgttgg
    21901 ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc tgtttatgaa
    21961 ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg aaaacattac
    22021 tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta caagcaattg
    22081 aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt tctgatacaa
    22141 agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc gaagtttatc
    22201 gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt ggtgaaaaac
    22261 tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa aaagaaaaag
    22321 aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat gacgataaaa
    22381 atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta ttcgctggcg
    22441 atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca gaagtgatgg
    22501 aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc accggagaca
    22561 acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt gttccttcaa
    22621 ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg ccagaagctg
    22681 aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat catgcttcag
    22741 gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact cacttaccaa
    22801 cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt gaaaaagcaa
    22861 tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa agcgagtatg
    22921 atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc cggacgtata
    22981 atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa aaattagatc
    23041 agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat gatcaaacat
    23101 caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct gtcacgggct
    23161 tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt atttttggaa
    23221 cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat ttcagaagaa
    23281 gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc acctcagtat
    23341 ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc aacactgatc
    23401 cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc tggtgtaccg
    23461 ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt gaaagcagca
    23521 agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga agtagcgaaa
    23581 gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac attaggacca
    23641 gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag tcggaatgaa
    23701 tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct ttttgctgag
    23761 aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt actaaaatca
    23821 aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa aaaaatattt
    23881 cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg caatgaacga
    23941 atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt tttttattat
    24001 aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac aagtttatcc
    24061 actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac tcagacaaaa
    24121 taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat atgatgaaac
    24181 agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt ttcctaccga
    24241 aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga aagtgttttc
    24301 tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt ttgaacaagt
    24361 aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga acttctggcc
    24421 tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag ttgtgacggg
    24481 aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag aagtaatcga
    24541 actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac caagtccaac
    24601 gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta ttgatgatgg
    24661 agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa cagccatgcc
    24721 tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca tcgaatctcc
    24781 tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc ctgggatgaa
    24841 atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg attggtcaac
    24901 tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa gccccgaaat
    24961 tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca actcagtaga
    25021 agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa cacttggatt
    25081 agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt acatgaatcg
    25141 cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt attgtcgcat
    25201 tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga aaaactaagc
    25261 gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga tgggaaaaat
    25321 aaaattgtag gaaaataaaa ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt
    25381 acaaaacgtt tgaccctgat ctttgggctg cgatcgccaa agaagaagaa agacaagaac
    25441 ataatttgga gctgattgcc tctgaaaact ttgtctccga agcagtgatg gctgcacaag
    25501 gaagtatctt gacaaataaa tacgcagaag gttatccagg gcatcgctat tatggtgggt
    25561 gtgaatttgt cgatatcgtt gaaaatttag cgattgaccg tgcaaaagaa ttattcggag
    25621 cgaaatttgc gaacgtccag cctcactccg gctctcaagc caacacggct gcttatctcg
    25681 ctttggtaga accaggggac acgatcttag gaatggatct ttccgcaggc ggacatctaa
    25741 cacatggatc accagtcaac ttcagtggca aaacctatca ctttgttgct tacggtgttg
    25801 atccgacaac agaagttatt gattataatg tggtacgtat tttagctaga aaacatcaac
    25861 caaaattgat cgtagcaggt gccagtgctt acggacgaac gatcgacttt gcgaaattca
    25921 gagaaatcgc agatgaagta ggcgcaaaac tgatggtaga tatggcacat atcgcaggat
    25981 tagtagcagc aggacttcat cctaatccag ttccttatgc agatatcaca acgactacca
    26041 ctcataaaac attgcgtggc cctcgaggcg gcatgatttt gacaaacgat gaagcattag
    26101 caaaaaaaat aaatagtgcg gtatttcctg gtattcaagg cggccctcta gagcatgtga
    26161 ttgcgggtaa agcagtagca ttcaaagaag cattagatcc tgcatttaaa gaatatagtg
    26221 aacaaatcat cgccaatgca aaagcaatgg tcaaagtttt caatcaagca attggaacca
    26281 gagtcatttc aggggcgaca gataatcatc tgatgttgat cgatgttcgc gagttaggta
    26341 taaatggaaa agaagcagag agcattttgg acagtgtgaa tatcactgta aataaaaact
    26401 cgattccttt tgaaacacta agtccattca aaacaagcgg tattcgaatc ggcacgccag
    26461 caatcactac tcgcgggttc aaagaagaag atgctgtaaa agtggctgaa ctagttgtca
    26521 aagcattaca agcaaaagat gataacgctc aactagacga agtcaaaaca ggtgtacgcg
    26581 aattaactga aaaatttccg ttgcataaaa aataaaaatt taaattccat tagtaaacag
    26641 aaaagttcaa tgttgggctt ttctgtttat tttttgggaa aacggattct ttgacaatat
    26701 cggtgatgct ctctagtata tgaggtgtaa ttttagtctt cacactaaat ctcagggagg
    26761 aaaagtcatg actgatcgta tctcgttaga aaaggaagca gtagcattca gtgatgcaaa
    26821 tgcgcctcat ccgcgtatct atgaactagc accggaagaa ggaagagttt tactagaaga
    26881 agtacaagaa tcacctgtga acaaatattc cgtcgatatt gaagacttga tggtggatac
    26941 tggcgaatgg ggaaaactca atattcgtct cgtacgacca gaagggaata tggataaatt
    27001 accagttatt ttttatattc atggagcagg ctgggttttt ggcagtgctc aaacccatga
    27061 taaattgatc agagaacttg cggttcgtac gaattccgtc gttgtctttc cagaatatag
    27121 tcgatcacca gaagcaaaat atccaacagc aattgaacaa aattatgctg tgttgcaaca
    27181 gctgaataaa ttagccaagt caaaaaatct ggatctcgag aatctaatcg ttgcaggaga
    27241 ttcagtaggt ggtaacatgg ctacagtcat gacgatctta acaaaagaac gaaagggcct
    27301 cccaatatcc aagcaattgc tgtattatcc agtcacaaac gctgattttg atacagattc
    27361 ctatcatgaa ttcggagaaa actatttttt gacaagagaa ggaatgatgt ggttttggga
    27421 ccaatatacg acgaatgaag aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa
    27481 tataaatgaa ctaaatgatc ttccttcagc aatgatttta acgggagaag cggatgtatt
    27541 aagagatgaa ggtgaggctt atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca
    27601 agtacgcttc caaggaatca tccatgattt tgtcatggta aatgctatgg atcagacaaa
    27661 ggcaacgcgt gcagcaatgg cattatctac ttcttggatc aagaaaaaag aataagcttt
    27721 gcttgtccgc aggaaaactt cttttcaaaa acggattttc ttaggaaaaa tggcgagttt
    27781 gactagtcaa tcacttgatt ttgttttaca attaaaaaga aacgacagaa caaaggagag
    27841 cttaaaaaat gggcaaattt caagtgatcg atcatccatt gattcaacac aaactaacta
    27901 ttattcggga caaaaactgc ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta
    27961 tgttgatggc atatgaagta tcacgggata tgccattgga agacatcgtg atcgaaacac
    28021 cgatcactga aacaacacaa aaaacgttgt ctggtaaaaa agtagcgatt attccaatct
    28081 tgcgcgcagg tattggaatg gttgacggta tcttggaatt gattccagct gctaaagtgg
    28141 gacatatcgg tctttatcga gatgaagaaa cattggaacc acatgaatat ttcgtcaaac
    28201 ttccagaaga cattgatgca cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg
    28261 gatcagcaat catggcaatc gatgcattga aagaacgtgg cgcgtcgaac attaaatttg
    28321 tttgtttggt agcagcacct gagggagtaa aagctttgca agaagcgcat cctgatattg
    28381 acatctatac agcttctcta gatgaaaaat tgaatgaaca tggttatatc gttcctggac
    28441 taggggatgc gggtgaccgt ttgtttggta caaaataagt aaatacgaaa aagggtgtga
    28501 cacaactttt gtcacatcct tttttggtca tttttaacgt ttgatgcaga acagcttttt
    28561 cacagctttc gtttgcatat aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa
    28621 atctttttta gttctttgta gttaacgatt actacaccct tactttgcgt catcaagttc
    28681 gcattttctg cagtttcatc aatctcaaca acagcggaat ttttcaattg ctttgaaatc
    28741 gttccagttt gcgacttgcc gtgaattgtg aacattacgt gagtattgat cttgaatttt
    28801 tcaccaattt ccgatggtac gatagttgta tcaaattttc caaaagttgc cattttcata
    28861 aaacctgcct ttcacatcaa ttataccacg ttttatgaaa aatatcatga aattaaacta
    28921 attttccatc ggactcttta caatcagcca aatagagtat ataatttctt ttggataagg
    28981 ccagcttagc tcagttggta gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc
    29041 ctatagctgg cattaaaaga gaagacagat atatcaagga tttttaatcc attgatacat
    29101 ctgtcttttt gtttaaagga aataaagtat gctttcttag caatagagca tgtattattt
    29161 ctataaaaat ttaggagaat aaaacgacta gcaatatagc tgtgaaatca aatatcacat
    29221 gaactgccca acttgtaatg atcgagttgc ttttaatggc agcttggttg aataacaatc
    29281 ttgccacccc ttgtatcaaa aggatgtgtg ctacagtgtg taaaatattt ccattatcat
    29341 aagtactgta atggataaat ccaaagatga ttgctgacaa gaaactagcg acaggaatgc
    29401 tgaacttact agaaaaaata gcgagaaaat acactgagaa caattcttct ccaagtatca
    29461 tgataggcaa aataataaat aataacgggc tgaaagcctc atttattgga ttgcctgtta
    29521 aaccccattt caaaatttgt tgcaaaaata ccgagactgc ggcactgatt actacattga
    29581 taaaaaaata tttggctata tttttgacag gagcgacagg tttcgagaac atttttttcg
    29641 tatctttttt accaaaaaca agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa
    29701 tgctaataat ggatggcgca aatagcaacc ctaacaaacc gagtacaacg acaatagtgc
    29761 ccaatatccc ttttttaagt tctatttttt ttcctaaaat gttattcatg ataataactc
    29821 ctactattca gtttttgaag atagagtcag tatacatgaa gagttaaaat aggcaatgtt
    29881 tttttatgga tatttgtatt gctaaaaatt tatcttatat attcattttg attatactac
    29941 ttttatttgt aaaaattttc tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt
    30001 ttttagatgt tccgagcgta cttcaatcat ttcaaaggag ttgctactta aatcgatatt
    30061 ttttaaatca gtatcaaaaa agctagcatt ttctaaatca ttagtagaaa acattatttt
    30121 agtgagggaa cagtctacaa aatctgcgct tgataagaca gagtgctcaa aggtactttc
    30181 ttttatgttt gaaccagata aatttacata gtccatttta caattctcga ataacgtgtt
    30241 cccaatataa ctttccatca atttagtacc tgttaattta caattgacaa atttcacgcg
    30301 atgcaacatg ctttttgtca atatacagcc tgaaaaatca cagttttcga atatacagtc
    30361 ggtaatctcc attctttgaa aatcattgga agcaaaaaat gcatacttga aaatagattt
    30421 gtaaataatt ggatggcaga ttatttggtt ttcgatgacc tctttttgtg aaaggatatt
    30481 atacagttca aaatcttcgt tttcatagat atgaaaaaag gatcctttat tcaagcttgc
    30541 ttcattaact ttaggaaaaa tcatatttaa ttacctccat tttcataaag tcatattaac
    30601 aaaagctaaa ccaacttaca ttagcagaaa atgagatagg ttttcgcctt tttcgataac
    30661 gatagtgtcg ctatctcttg aaacgttctt agaattgccc tgagagacac aaattaggcg
    30721 taattctaat aaaaatctta gaataaaaat ttttactgat ttaatttaga atgtttttaa
    30781 aataaaaatc gttgccataa cagtgtattg aaaggtttta acaatccagt aatgatttaa
    30841 aaaaatctag atgtagcgta tactttgttt atagaatgta gtgggaagaa atatattttt
    30901 gggaggaaat cgtgatgtct aaaagtgaag aaatagaaaa catggtacta aaattatatg
    30961 atctcgtttt aaatccagag atcaaagaaa aggaacgtat acttttgata gacgcaaaga
    31021 caggcttaga aaaaggtcag tattatccta aagtcataaa taatttggaa cgtagtttac
    31081 gtccgttagc aatcagggga gaattgtcta aaccagtatc accgttttat atggagatat
    31141 caacaatagg aaaatttgaa aaagaattag gtcgaggaat ggcttcggca ccgattactt
    31201 ttgggcattt ataaaaacga gttattcatc tcaatgaatt aaaaagtata gtcaatctca
    31261 taatgcaggc taggttcatt tatttgaaca gagtctgtat ttttttattc taaataaaga
    31321 aacagatgtt tctttatatt gtattttatc ctttaattaa cacaaaaaca gctagtataa
    31381 actaataatg ttaattaaaa gtgtatttta
//
LOCUS       DACJGB010000029        31014 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.029, whole genome shotgun sequence.
ACCESSION   DACJGB010000029 DACJGB010000000
VERSION     DACJGB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.029"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(405..2480)
                     /locus_tag="IYI34_001370"
     CDS             complement(405..2480)
                     /locus_tag="IYI34_001370"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAR0559244.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="IYI34_001371"
     CDS             complement(2482..3399)
                     /gene="glyQ"
                     /locus_tag="IYI34_001371"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAR0559245.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3783..4595)
                     /gene="recO"
                     /locus_tag="IYI34_001372"
     CDS             complement(3783..4595)
                     /gene="recO"
                     /locus_tag="IYI34_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAR0559246.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4738..5637)
                     /locus_tag="IYI34_001373"
     CDS             complement(4738..5637)
                     /locus_tag="IYI34_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAR0559247.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5652..6053)
                     /locus_tag="IYI34_001374"
     CDS             complement(5652..6053)
                     /locus_tag="IYI34_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAR0559248.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="IYI34_001375"
     CDS             complement(6031..6507)
                     /gene="ybeY"
                     /locus_tag="IYI34_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAR0559249.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6524..8716)
                     /locus_tag="IYI34_001376"
     CDS             complement(6524..8716)
                     /locus_tag="IYI34_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAR0559250.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8740..9711)
                     /locus_tag="IYI34_001377"
     CDS             complement(8740..9711)
                     /locus_tag="IYI34_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAR0559251.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10417..11796
                     /gene="brnQ"
                     /locus_tag="IYI34_001378"
     CDS             10417..11796
                     /gene="brnQ"
                     /locus_tag="IYI34_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAR0559252.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11952..12398)
                     /locus_tag="IYI34_001379"
     CDS             complement(11952..12398)
                     /locus_tag="IYI34_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAR0559253.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12425..12601)
                     /locus_tag="IYI34_001380"
     CDS             complement(12425..12601)
                     /locus_tag="IYI34_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAR0559254.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12763..13191)
                     /locus_tag="IYI34_001381"
     CDS             complement(12763..13191)
                     /locus_tag="IYI34_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAR0559255.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13283..14143
                     /locus_tag="IYI34_001382"
     CDS             13283..14143
                     /locus_tag="IYI34_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAR0559256.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14227..14520)
                     /locus_tag="IYI34_001383"
     CDS             complement(14227..14520)
                     /locus_tag="IYI34_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAR0559257.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAIIQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(14593..16320)
                     /locus_tag="IYI34_001384"
     CDS             complement(14593..16320)
                     /locus_tag="IYI34_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAR0559258.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="IYI34_001385"
     CDS             complement(16533..17459)
                     /gene="trxB"
                     /locus_tag="IYI34_001385"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAR0559259.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17604..19037)
                     /gene="ascB"
                     /locus_tag="IYI34_001386"
     CDS             complement(17604..19037)
                     /gene="ascB"
                     /locus_tag="IYI34_001386"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAR0559260.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19037..20440)
                     /locus_tag="IYI34_001387"
     CDS             complement(19037..20440)
                     /locus_tag="IYI34_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAR0559261.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20670..21572
                     /locus_tag="IYI34_001388"
     CDS             20670..21572
                     /locus_tag="IYI34_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR0559262.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21613..23499)
                     /locus_tag="IYI34_001389"
     CDS             complement(21613..23499)
                     /locus_tag="IYI34_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0559263.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23596..24447)
                     /locus_tag="IYI34_001390"
     CDS             complement(23596..24447)
                     /locus_tag="IYI34_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0559264.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24905..26689)
                     /locus_tag="IYI34_001391"
     CDS             complement(24905..26689)
                     /locus_tag="IYI34_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559265.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26689..28440)
                     /locus_tag="IYI34_001392"
     CDS             complement(26689..28440)
                     /locus_tag="IYI34_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0559266.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28582..28806)
                     /locus_tag="IYI34_001393"
     CDS             complement(28582..28806)
                     /locus_tag="IYI34_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAR0559267.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            29057..30643
                     /locus_tag="IYI34_001394"
     CDS             29057..30643
                     /locus_tag="IYI34_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAR0559268.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8675 a         6650 c         5522 g        10167 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agcctatttt gtgcctattc tctggtttat ttacatatta gaaattattt tgtgatgatt
      421 tgatctaaac ttgctagcga caaagccatg gcagcaattt gacttaattg cgttaatcgg
      481 ttcttacgta ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc
      541 agtgggcgaa ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt
      601 tttttcattg cctctactgc tttgtgcagt tctttctccg cttcattttc gaacagttca
      661 gggtctactt ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc
      721 aaagcttcca tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct
      781 gctgcaaata cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca
      841 tgacgaatat tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc
      901 tgttgttgcc cttctgataa accaatgccg aaacgatccg tatctttact gataacatca
      961 ctaatcgttt cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgatccga
     1021 acgacaccgt atgcttggcg acgtaaggca taaggatcgt ttgacccgct tggaatcatc
     1081 ccaactgcaa agaaagaaaa aatgctatct aatttatctg caatcgctaa aacagaacct
     1141 actgtagact gaggtagctc tccttcactg gatataggca tgtaatgctc acgaatcgct
     1201 gttgctacag caggtttctc gcctttcagc agagcatatt tttcgcccat gatcccttgt
     1261 agttcaggaa attcccctac catgttggtt actagatcaa atttatagat ttcagcagct
     1321 cgttgcagat ccatcaattc ttcctcagat agtcctactt gttcgccaat gatacgggca
     1381 atgacttgta cccgctgcat tttctcatag atactaccga ttttttcatg gaaagtaaca
     1441 gtttttagtt tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat
     1501 tccgcatctt ctaaacgagc cgtcaatact ttttgattgc ctcgaaccac attttccaat
     1561 tttacccgat caccattacg agcagaaata aaatgaggaa gcagcatacc ttgatcgttc
     1621 cgtacctcga aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca
     1681 gaaagatatt tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg
     1741 acttcttcta gtaaatcttc gtctagatca ataacccagt gatttttttc ggctaattca
     1801 tttgcttgtg taacgatcat ttcttgtctt ttttgcggtg aagcaataac gaattgttca
     1861 ttcaattttt cttcatattc ttttgcatgc gcaaatgtca catcttcgcc taagaaacga
     1921 tgtcctcgag aggctctgcc tgttttgaca tctaagatct caaacgggat tacttcgtca
     1981 tctaacaatg ccacgatcca gtgaataggg cggatatatt caaaatcgta ttctgcccaa
     2041 tgcatcgtta ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct
     2101 ttcgcttctt tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct
     2161 ctaaaagtga tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc
     2221 gtccagtttc catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttca
     2281 ctgtctgctt gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa
     2341 aacgtttcga tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct
     2401 tctaattgtt tcatgcttgg tgtgacaaca tgagcaggca tttcttccag cccgatttca
     2461 agtaataagt cttttgccat cttattcagc ctccttcatg ttttctggat ttgctgtgtt
     2521 tttattcaat aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc
     2581 acgagccatg ttgcggatac gggaaagata acctgctcgt tctgttacgg atactgcacc
     2641 gcgagcatct aataaattga atgtatggct gcatttcaaa atataatcat acgctggatg
     2701 aacaagattt tcatcgatac agcgtttagc ttctttttca aaacggtcaa agttgcttag
     2761 tagcaagtct tggtcactta cttcaaaaga atatttggaa tgttcatatt ctggttgttt
     2821 gaagatctcg ccatatttca caccatcagc ccattccaga tcatagacac tttcaacttc
     2881 ttgaatatat gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca
     2941 aggtagtcct ccgacttgct ggaagtacgt gaattgtgtg atctccatcc catcaagcca
     3001 tacttcccaa cccacaccgg cacaccccat cgatgggttc tcccagttgt cttcaacaaa
     3061 acgaatatcg tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc
     3121 ttggatattt tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg
     3181 gtttgggttt tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc
     3241 attccatggt tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc
     3301 tttttctgtg tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa
     3361 tgttaaaatc atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt
     3421 ttaagcgttg ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga
     3481 ttacagacat agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc
     3541 agcttcattt atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact
     3601 ctcccgaact cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt
     3661 tgcagttttc ttgtttatat aatagatcct ctcagtgaac gatctattta atctacggtt
     3721 aataatagac ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc
     3781 atttattctt ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat
     3841 acgtgtgccc aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca
     3901 tattcatcat acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag
     3961 tggattccac tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg
     4021 cgaggatctg catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg
     4081 ccagatttgg aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc
     4141 caaattggcg cgattccgaa gcgttgcagt atttgaactt caaagatatt cgtgatgatt
     4201 tctgcatcat ctccttgatc catccgctgc agagcttgtt gcgtaaattg gaataaatgc
     4261 gggtcatatt gatgatcttc gattgctgca tctactagat ttagcaaata ggtaccataa
     4321 ccgctcaaaa agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt
     4381 aaaaacgaca aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc
     4441 aaagccggag ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg
     4501 ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat
     4561 aaaatcaagc ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct
     4621 ggaaaagcag attctgccga tccagattat ttatgtttcg atcttccgcc gtttttctca
     4681 acgtgcggat ttttatccag tttatttcta tacttaacct ataagaagtt cagaatatca
     4741 gtattcgtct tttcggtagc caaagtcttg cagatagact tttttgtctc gccagtcttt
     4801 ttggactttg acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt
     4861 tcttgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt
     4921 ttggctatct ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg
     4981 cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa
     5041 gactttctca cggatcaatt cagaaacgat aaagtattca ggatgatcag tcacttgatc
     5101 atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc
     5161 gacattgttt ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga
     5221 ataatcttcg atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat
     5281 cagataaact ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc
     5341 gcgtttttga tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta
     5401 agctgcttct accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc
     5461 gataaaaatc agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt
     5521 ctgtgccttg tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga
     5581 ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata
     5641 cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca
     5701 atgatgacag caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt
     5761 ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta
     5821 ttgatgatct ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc
     5881 cgatcaagct ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc
     5941 ctcatattgc gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac
     6001 atcaggaaat gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat
     6061 ctctttctgt aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt
     6121 gatatgcaag aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta
     6181 agcttctgct tgttcagcag cacgttcaat agaaatcatg atgtctccta gtacacgagg
     6241 caaaggattc tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag
     6301 agcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg
     6361 gattgccgca ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata
     6421 agatgctgca aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt
     6481 tgtttcatcc ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt
     6541 cctttatctt ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc
     6601 tacttaaacc gctgatcaat gatttttcca cttttcggat ttctttcaag gtcagtccac
     6661 tatcgtctaa ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga
     6721 ttttttcgct cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat
     6781 tgacgatccc tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg
     6841 ttacttccgg attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat
     6901 gatgctgttt gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg
     6961 ctccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt
     7021 gcgggttttc cgctcctgaa ggcaagttct caacgaaaaa gctagcatgt ttgatcttgc
     7081 cgatatcatg ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta
     7141 cagcgttagc acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt
     7201 ccagcaactg tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac
     7261 tgtcatcatt gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga
     7321 agagtgcacc agtgaacccg cagacaagca ttaaccaagt cgttgcatca ccaaagctca
     7381 ttccttgata gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg
     7441 cagcagataa tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag
     7501 agaaaacata agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa
     7561 tgaaaagtgc agatactgct tggaaaatac cagccatgat ccctgctctt cggttcaaga
     7621 aaaagcttaa aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt
     7681 gttccgtttg gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca
     7741 catagaaaag cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa
     7801 tcagtacgcc gatttgaagc aaaacagcta aaaccatggc aatgagtggg aaaatcgaag
     7861 tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca tcaatttgat
     7921 ttccttcgcg aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg
     7981 cagattgttt taattcttct gtctttttct cattcaatac atcattgact acaatgcctt
     8041 gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa tactgaatct
     8101 gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt ttactcatct
     8161 gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt tcagtcattt
     8221 caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg ttttctgcat
     8281 tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta ggtttcggga
     8341 tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg atggtatcat
     8401 tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca tctgccagat
     8461 ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt ctttgttcgg
     8521 tttcttccgt gttctcgatc gttttattcg cacggatact ctcttctgct acttggcctt
     8581 ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag acaatgataa
     8641 aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag tttttcttgt agtttctgca
     8701 gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat gaagatccgc
     8761 cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc
     8821 aaaattaaca aaatcgattt ttttgatttg ctttagtgtt cgttctgcat gaacaagacc
     8881 acttgtcgtc cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga
     8941 ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc
     9001 gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta atggtgcgat
     9061 ttcgattacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa
     9121 agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc cgggtagaaa
     9181 gcctaggttt tcccctgcct ctacagcggg ccgcgtcaaa atgatttttt gcacttcgcc
     9241 tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg ttccggctgg
     9301 ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct tttgaccgct
     9361 gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc
     9421 aacaaaataa tctaatgtac ctttttgccc cattttcaat gccgtcacga catctggtgt
     9481 acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa
     9541 ggaaatctgg gattgttcac caatgatctg aatgatttct ccacgtgtat ggatggttgt
     9601 ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac
     9661 atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca
     9721 tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca
     9781 aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg ttaaaaaata
     9841 gctgtttcca ttcgctaaag gtctcgtttt gataaaaaga atctgagaat gtaagttata
     9901 tgaaagtgga gaacaaaata ttccgtttca ttcagaggta ttctctaggt atttttcttg
     9961 tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa aacaggtgta
    10021 ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag gctattcaac
    10081 gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata gacgaacatc
    10141 atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat caggctagcg
    10201 gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa aaaaggtggt
    10261 accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt tatattttaa
    10321 ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca acgtttatat
    10381 cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca
    10441 tggaaacaac tattgatggt cagttcactt atcttcggga tgtttttcgg gtcaggtaat
    10501 ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag tgctggcgta
    10561 ggatttgcca tctcaggcgc tcttttccca ttactagcta tcttagctgt agtagtgaca
    10621 aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc
    10681 ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac agctgctact
    10741 gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt aggtatgttt
    10801 ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc cacacggttg
    10861 gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattattagc aatcattttt
    10921 gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga tacttaccaa
    10981 acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc agtagccttg
    11041 cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa agataaaaaa
    11101 gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt catgatctat
    11161 tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc
    11221 ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc tagcttctta
    11281 ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt ttcttttgca
    11341 caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact
    11401 ttcgtctctt ttgtcgttgc aaatgctggt ttggacaata tcatccaatg gtctttacca
    11461 gtattgatgc ttttgtatcc attgtcgctt gcgttgattt tgctttcctt aacagcaaaa
    11521 tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc tgttcccgcc
    11581 gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt tgtagcaagc
    11641 atgctcgaat tttatcatca tcacgttcct tttgctgcat tgggacttgg ctggatggtc
    11701 ccaacgttac ttgggtatgc tggcagctta ctcttctatt acgcctatcg cctatcaggg
    11761 tataaacaag aagcaaatga actaccagaa gaataaccag atcaaacaca atttaacata
    11821 agaaatatta aacaggaaaa aaacagctag atgaaagaat cctattcttt cgtctagcta
    11881 ttttttagat ctctttttta cgagcacaaa aaaactgcgg acaatgattg tccgcagttt
    11941 aaatgataaa attaagattg taatagttct ttcacaatgg catttacttg gtttccatct
    12001 gcttttcctt tgactttagg catgatcgca cccatgactt tgccaaattc tttcattgat
    12061 gaagcaccag tttgcgtgat tgcttcttga acaagttgtc gaatttcttc gtcggaaagt
    12121 tgttccggca tgtatttttc gactatcaca atttcacttt ttactttttc agctaaatcg
    12181 tcacgtcctg cttcttcaaa ctcatgcaaa gaatcacgtc gctgtttcat ttcgcgagac
    12241 aacaccgtta attcttcttc ttctgtcaag tcgtgacctt ctttaatctg ctcgttttgt
    12301 atagatgact tgatcattcg gatgacttgt aaagattcct tatctttcgc tttcatcgct
    12361 gttttcatgt catcgtttaa tctacttagt agtgacacac taccaactcc aattcatcac
    12421 agaactagaa ttttttgcgt tttctagctg cttcagattt tttcttgcgt cttacacttg
    12481 gtttttcgta gaattcacgt ttacgagatt cttgtaaagt acccgctttt gacacggaac
    12541 gtttgaagcg acgaagagca tcgtcaagtg attcgttttt gcgaacgact gtttttgaca
    12601 tgttaattcc ctccctccga gcttaaaaag taaccgtaat tgttattgaa atatatctca
    12661 aaaaacaaga ctgtcctgta aatattatat ctaatgctgt tttgaccgtc aaggaaatct
    12721 tgcttttttt taaaattatc aaatgttatt ttatttaaat ccttattgac aattttcaca
    12781 tcttccgaaa atctcgaatc tatgcccttc gatcgtacat ccaccgagct gttcttcgaa
    12841 ataattcatt ggacacatat ggatttcttt cgttgttccg cacaccgtac agataaagtg
    12901 atggtgatgg ccgatttccc cttgacagca atgaaaacga aacttcatct ctccatttaa
    12961 atcggtttct tccagtaaat cgatttctgc aaaatcatgg agatttcgat aaatcgtatc
    13021 gtaacttact cctttgaatt cctgattcat atagtcaaaa acttctttag cggatacgta
    13081 gcgattgcgc ttcactaaat aagttaacaa tgcttctctt tttttagtat atttaaaacc
    13141 gttatttttc aatgtttcta gtgattgttc gatcaacgtc tgttttttca tgacttattc
    13201 ctccaaatca aaatgattac gaagtacaca ttctagagta tggtataata aacataaaaa
    13261 aaatgcaagt gaggttttat ccatgtcttc cgattctaca gaaatcgtta ctttttttgt
    13321 catctccgac tctgctggag aaactgcgac aaaactagcg caagccacta tggcccaata
    13381 tcctagcgtt gagttcaatt tattccgcag aacatttgtg acagatgaga aaacattgat
    13441 gcaggcactg caagatgcac tagaagaaaa agctcttgtc ctgcacacat tgatcaatca
    13501 agaattgatc gagatgacgc gttctttttg tgaagaacat caattattta gttttgatgt
    13561 gatgactccg ccgatatcag aaatcgagcg cttaactggt atcaagccca ttcggcaacc
    13621 tggcgccctt catttattga atgagaacta tttcaaacgg atcaaagcaa tggaatttgc
    13681 tgtaaaatac gatgacggga aagatcctcg tgggtttcta gaagcagatg tcgttttgct
    13741 aggcgtatcc cgaacttcca aaacgccttt gagcttgttt ttagctaaca aaaatttaaa
    13801 agttgccaat ctacctttga tcccacaagc acatattccc aaacagcttt gggagatcga
    13861 tcctaaaaaa atcgtgggac tgacgaataa cccagatatt ttaaacaata tccgtaaaga
    13921 acggatgcgc tcttacggat taaatcctga tactgcttat tccgatatcg aaaaaatacg
    13981 agctgaactc gattttgcga atgatcttta tgaaaaattg ggctgcgtag taatcaatgt
    14041 cgcttcttta tctattgaag aaacagcttc gcttattttg aatgctttag acctagaaga
    14101 ccatagttat tatggaactg aaaccaccga agaaacagac taacaatttt tctttaaaaa
    14161 aactggactg tgtcagcgaa gaagattcgt tgacacagtc cagtttgtct ctatttgcac
    14221 ctatcgttat tgatgttcca catgtttgag tgtctggttc aaaatatcca gtacgtgaga
    14281 atcatcaaga ctatacaaaa tcgcttttcc ctcacgatgg gacttgacga catggttttc
    14341 tcttaataaa cgcagttgat gcgatacagc agattgttcc atttccactg cttggatgat
    14401 tgctgtcaca ttttgttccc cgtctttcag atacagcaga attttgagac gagtggggtc
    14461 actcaacatt ttgaatagtt ggctgacttt ctctactttt gttttttgcg tgtctggcaa
    14521 tccattcacc tccaacttga aaaaagactg acttctaaag tttaccactt tttccatcag
    14581 tctcaagttt ttttattctt ctgtcatctt ttggataatc gtttcataag cggctacttt
    14641 ttcagctgcg tcttctgaag agtttccttt tacaccgata taaaatttga ttttcggctc
    14701 agttcctgaa ggacggatag cgatccagcc attttcttcc agcgtatact tcaatacgtc
    14761 agatggcggt gtcgttagtt tttctacgtt tccttcacgg tctgttctcg tcaactcttt
    14821 gaagtcttct gtttggatga cttttgctcc ggcaaattct gttggtgctt cttcacggaa
    14881 tttttccatc aaagcagaga ttttcgcaga accttcttgt ccgctcattg tcacagaaat
    14941 cgttttttct gcgtagtagc cataggtttc aaaaatcgtt tgcaagccat catacaacgt
    15001 tttgccttgt tttttatagt aagctgctac ttctgctaat aagagcaatg cttggatcgc
    15061 gtctttgtcg cgaacgaaag gtttaactaa ataaccgtaa ctttcttcga atccgaacat
    15121 aaaagtatgg gaatgatctt cttcaaattg ttgaattttt tcagcaatga atttgaatcc
    15181 tgtcaataca ttgaacatgg tagccccata gtgttccgca attgctgtag gtaattcgct
    15241 agaaacgatc gatttcaata ccgctgcgtt ttcaggtaaa gtgcctcgcg attggtgtgc
    15301 ttccaaaata tattgagcaa acagtgctcc gatttggttt ccggtcaata cttcatagga
    15361 gccatttggc aaacgaacgg ctgcacctaa acggtctgca tcaggatctg ttgcaatcag
    15421 caaatcagcg ccttctttct cgccaagtcg tatcgcatac tcaaaagcag agtgttcttc
    15481 tggatttggt gatttgactg tagtgaaatc tgggtctgcg acagcttgtt ctggaactag
    15541 tacgaattgt tcgaatccag cttgtttcaa cgcacgttcg cccaacattt ttcctgttcc
    15601 gtgaagtggt gtgtacacaa gtttcaaatc tttccccatt gtttccacca attcatgatc
    15661 aatcgttact gctttgattt cttctagata agcggcatcc acttcttctc cgatgatcgt
    15721 gatcaatccg ctatgttcag cttcttcgtc agataatact tcaactttta acggattgct
    15781 gacttcacgg acatatttcg tcaatgcatc tgcgtctgca ggaggcattt gtcccccatc
    15841 ttctccgtag actttatatc cgttgtaagc tgctggattg tgggatgcgg ttaccatgat
    15901 tcccgcaaac gtcttcaaat gacggactgc aaaagaaagc tctggtgttg gacgtagact
    15961 ttcgaataca taagatggga tattgtgatg tgctaatgtt ttggctgctt ccatggcaaa
    16021 ttctggtgat tgatggcgtg agtcataagc gatagcgaca cctcggcgtt ttgtttcttc
    16081 gccttgtgta tccatgaaac gtgcaagtcc ttctgttgct tgtcttaccg tatagatgtt
    16141 cattcgattg atcccaggcc ctaagattcc ccgcatgcct gcagtaccga attccaacgg
    16201 tgcataaaaa gcatcttcaa gtttttcagg atcttcaccc agatcattta attgtttttt
    16261 taagttttct tctaaatttt cttcatgtac ccattgttca tacacttgtt cccaagacat
    16321 gaaaataaca cctctttctg aaatatggtt cttaataagt atagcattca cttattagat
    16381 atgaaagtat gaggaccaaa aaacgcttaa atcaagaaag gattctttta tcaagcacgg
    16441 ttgcaagtat tcgcataaat cggattcagt agatttcacg cgaaaaaaaa gagtaaaaga
    16501 acaattaaac cgttcttcca ctctttttaa acttatgcag ttgtttcttc taaagattcc
    16561 aagtatttat acacttgttg tccagcgatc ccaccatcac caacagcagt agtgatttgt
    16621 cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc ctggtatatt cgtcttcatt
    16681 tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc ctgcttctct aaatggtgca
    16741 gttaatggat ccagtccaac atagatgaat actccgtctg ctgccaaatc aaactcattg
    16801 ccggttttga cattacgtgc tttcacacct gtcactacca tttcattccc aacgatttcg
    16861 tcaacgactg tatcccacat aaaggaaatt ttttcattcg caaatgcacg gtcttggatg
    16921 atcttttgtg cacgtaattc atctctacgg tggacgatga ctacttcaga agcaaattgt
    16981 gtcaaataga tggcttcttc tactgctgag tctcctcctc cgatcactac tagacgtttg
    17041 ttacggaaaa acgcaccatc acaaactgca cagtaagaaa cgccacgtcc agcaaattct
    17101 tcctcgccag gtacgcctaa tttacggtgg atacagcctg ttgcgataat gactgttttt
    17161 ccttcgtaag ttttatcttc acaaataact tctttgtggc tgccgtgatc cttgatatct
    17221 tgcacgattc cataagcatt ttctgtccca aatcttgaaa caccttcata catcttataa
    17281 gctagatcag gacccattat tgaatcgaaa ccaggatagt tttcgatttc tgccgtattg
    17341 ttcatttggc cgccaggggc gccttgttcg atcatcaata cggataagtt tgaacgtgaa
    17401 gcatataaag cagcagtcat cccagcagga cctgcgccaa taacgattac atcatacata
    17461 tgtagattct cctttactta atcaagtaag catactttac agccaatgcg aaaaaaagtc
    17521 tattgttctg cttgcgtttc tcgaatagct ttattatact cgaaaaatcc gctgaaaaac
    17581 aatagacttt tataaatcat tgatcaatca ttcgaaagat tttctccatt tgaatcaatt
    17641 acttttttat accagttgaa tgatttcttc ttcgaacggg ctaatgtgcc atttccttcg
    17701 ttgtcacgat cgacataaat gaatccgtac cttttcttca tctcacctgt accagctgat
    17761 actaaatcaa tacatcccca agtcgtatag cctaatagat cgactccatc taattctact
    17821 gcatctttca ttgcctggat atgtgctgcc agatagtcga ttcgatagtc atcttccaca
    17881 taaccattct catcgggcgt atcgactgca cctaagccgt tttctacaat aaatagtggt
    17941 ttttgataac gatcataaat atcattcatc gttattctca agcctaatgg atcaatttgc
    18001 caaccccatt cacttgcttc cagatatgga tttttgacag aagcaaagat atttcctgca
    18061 gtctgttcca agagttcgtt gtcagtagtc gaaactcgag aagaataata agaaaaggag
    18121 ataaaatcaa cagtatgttc tttcagcaat gcggcatccc caggttccat tttgatctga
    18181 atgccatttc gttccatttc tttcaatgca taagcaggat attctcctcg tgactggaca
    18241 tcaatgaaga aataattttc tcgatctgat tttctagaag cccaaacatc ttcaggattg
    18301 caagtgtatg gataatatgc tcctgctgcc agcatacagc ctactttgtt ctccggatct
    18361 acttcatgag caatcttcgt tgcgatggca cttgcaacca actcatgatg tgccgcttga
    18421 tatttgattt gttcttcatt ttctccttct tcaaaataca gccctgcgcc catgaatggt
    18481 gcatgaagga tcatattgat ctcattgaat gtcaaccaat atttgacaag tcctttatag
    18541 cgattgaaga tgactcggca cagattttca taaaaaccaa cgagttctct ggaacgccac
    18601 gcaccatatt ctttgatcaa atgcatcgga caatcaaaat gtgtaatcgt cacaagcggc
    18661 tcgatcccgt acttttgaca ttctttaaag acatcctcat aaaatttcaa accagattca
    18721 tttggctctt tttcatcgcc cattggaaaa atacgactcc aagcaatgga catccgataa
    18781 gtcttgaacc ccatttctgc aaataaagca atatcttcct tataatgatg atacatatcg
    18841 atcgcaattt ttgctggata aaaatgattt tcatcaaatt caaacatttt cttttttcca
    18901 gtaatgacag gaaaacggtc ttctccgatt ggcacaacat ctacgttcgc taacccacgt
    18961 ccgccttcat cataaccgcc ctcgcattga ttggctgctg tagctccgcc ccataaaaaa
    19021 tcttttctga atcccatcat acttcctcca ttacatgtgt attgcttgat aattctcttg
    19081 ccagttcatc ttggacttcg attccttctg cttttaaccg gctgattgtt tccttgaatt
    19141 gcggtaaata ctgctgatgt gcaataaaca actcattcat gatccgtttt gctgtctttc
    19201 ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc tgttccataa tcaccagtca
    19261 cagctgcttc gatcgtcagc aattccatat tcttcattac ttgcaaccag cctctttcag
    19321 cagcaggaag ggaaccaaac gctacatttc gtgcgccttg tgcgccaaca taggcagtga
    19381 cttctactac acaatctgcc ggcagatcag gaacagctcc atcattcttt gtagatacca
    19441 cgatctgtgt ttctttattt ccatagatcg aagcaatcgt ttcacaggca gcatctgaat
    19501 aataagcacc acctcgtttt tgcaattgtt caggtttatg atccaattct ggatctttgt
    19561 ataattcaaa cagctctgct tctgtttctt tcacttgctg cgctcttgta ccaatcgttt
    19621 gatattcttc taaagcatgt tccagcattt cttcttcacg gtagtaatag cggtggtagc
    19681 cacaagggat catgttcatt tgttttaatt gttctttgaa aaacgggata tcaaagatat
    19741 ttttcggcaa tccattgtct tcatcgtata aacgatcaat caattctgcc gtgcggtcat
    19801 ttcctttatt atctgctact ttgtgccaat ggaagtgatt tagaccggca aacttgtaga
    19861 tcagctcttc ttttttcttg ccaagcattg ttggttctgt catcatcgcc attactggca
    19921 cattgcataa tcctatcact ttatcccatt tgccatatct gataactgct tctgtcacca
    19981 tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg acataaacgc ttcatgtctt
    20041 caacgattgc caaaaccact ggaacggtac gaaaagcttt gaatatcccg ccagcaccat
    20101 ttgtttcttg cccaatcatc ccataataac tagggatccg ctcgtctttg attcgtgcat
    20161 ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc atctttcaat gcttcttccc
    20221 gatccagagt tgcatagatt ttgacttcat acggagaagc atcccacata cgctgagaca
    20281 tctgactgac gatctcaagc ttttcttttc cttcttctac atctacgagc cagatttctc
    20341 gtatcggtaa ttcctcatat cttttgataa atccttccat caactcgggg gtgtaactgc
    20401 tgcctccacc aatcgtagcg attttgattc cttttgccat gttctcactc ctttactttt
    20461 tacactttta ttatcttttt tgtaaacaaa acaaacaata gaaattcgtt tctttccagt
    20521 aacatttaca aaagttagtg ttttctactg gatttttgta aatatcttgt caaaaaatgt
    20581 ctttattaag ctatataaca taaatccgtt tacaaatcat tttcaaaaaa gttataattt
    20641 acgtaaaaaa acaagaagaa ggtatctaca tgttattacg tgaaaaaatc aaagaaatct
    20701 ccttttctcc ttcagaaaat gaagtgattc agtttctctt aagcgattct ccagcacttg
    20761 gcgaaatgac catccaagag attgctgagc gatgttatgt ccacccgtcc acactcatcc
    20821 gtgtagcaaa aaaaatgggt tttgatggct gggtcagttt tcgtaatgct tttttagctg
    20881 aaacagccta tcttgaaggg aattttcaag acgtagatgc gaatcttcct ttttccacca
    20941 cagacggtat tatgacgatc gcaaataaaa tggcttcttt agaacagatg acgattcaag
    21001 atacactttc tctcattcat catgaccaac tgcaaaaagc caaacaacta atgcttcaag
    21061 caaaaaaaat cgtgattttc gccagtaact cgaatacttt gatttctcaa gatttcatgc
    21121 tgaaaatgaa gcgcatcaaa atggatgtca cgattgtcac cacttttggt gaatcgttct
    21181 atgaagcgta caattgcacg agtgatatgt gcgggattct gatttcttat acgggtgaaa
    21241 acaatgtgat ccttcgtacc gcagaaatat tgaaacaaaa tcatgttcca attttagctt
    21301 tgaccagtat cggtgattca acgctatctt caatgagtac agccgctttg cgacttacga
    21361 cacgcgaacg actgtattcg aaaattggta attttacgat caatacatcg atttgttatc
    21421 ttctagacgt gttatatagt tgtgtttttg cagaaaatta tcaacagaat ttagtccatc
    21481 taatttctgt aggacaaaaa tttgataaac gccctatcag ctcagccatc atgaaagagc
    21541 ctttcaattt tgaaaataaa gaagtggact aaaaagaata attaagccta gtccattctt
    21601 tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg tctggcccga ccgtctcccc
    21661 ttgattttcc aaaatatcca aataatccgt gtaattggtg acgatcacag gtgtttctaa
    21721 actgtatcct tcctcttcga ttttcttttt gtcgaagcgg agcaacagat cgccttgttt
    21781 cactctttct ccttgctgca ccaagctttc aaaatatttc ccttccaact gaacagtatc
    21841 caatccgaca tgaatcagca attccagacc tgcttcagaa accattccga ttgcatgttt
    21901 cgtcgggaac agggtcatga ctgttccgtc aaaaggggca cgtacttctc ctttttcagg
    21961 gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa gctgcatcct ttgctgcatt
    22021 cagtggagcg atttgtcctt gtatcggtgt ttttacaatt tctttacctg atcttttctg
    22081 tctatcatct gtttcacctg cttcctcctt ccaaaagaaa aaagtcagcg caaaggatac
    22141 caccatcgca atggcgattc caatcaatac attaatcaca tccgttaaat caccatctgg
    22201 tctaatatag ttgggaattg taaagatgcc taatccacct ggattccaag aagttaaatt
    22261 cattaatcca ctatacgcgc cccctaacgc accaccgaca caggtaaaaa taaatatttt
    22321 tttacgtggc aatgtaaacc cataaatagc tggctctgta atccccacaa taccagaaac
    22381 aatggctggt attgcgattg ctttgtcttt tgggtttcta agcttaaagt agagtgcagt
    22441 aagtgctgca gtttgtccaa aagacactac tgcaacaggt gtcatgatat tttgccaacc
    22501 attcacagaa aattgcatca atcccattgg tacgattgcc caatggagac caaacatcac
    22561 tagaatttgc cagaagaagc ctacgattgc tccataaata actggcgaaa gtgctaaaag
    22621 tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg aatgctggtc caacgagtaa
    22681 aaaacctaag ggcattgtaa taagaacggt gaagaatgga acgaagaaat tcgcgatcat
    22741 ctcaggtact aactttttaa ttggtttctg aatttggcta gctaacaaca caataaagat
    22801 aatgggaatg acagaagaag cgtagttacg cgcaatccag ggaatgccga atactgtttg
    22861 ataaataggt gcttcaaaaa tagtgccaga aaatatggta gtcagtggtg cttctgctgc
    22921 accactcaaa gcttccagcg acaagctagg attcattaat gctgccccaa gcaacatccc
    22981 tacgaattca tttactttaa atttacgggc agctgttaaa ccgataataa caggcataaa
    23041 ttggaataca gaatcaccga tattatcaaa cataacaaat gtgccagaac cgcggtcaaa
    23101 tgcgcctaag aagacaagta atgctgtcag tccacgaatc atcccaccag cagctagaat
    23161 agcaaggaat ggctggaaac agcccgatat tccatcaata agcatatcaa acggtctcat
    23221 tttttctgtt gaaacagcct catcaaaagt aaggtttcct gcttttataa cctcttcaaa
    23281 cacgactggc acatgattcc cgatgacaac ttggtactgt cctcccgctc gcattaccgt
    23341 cacaacaccg tccatatttt tcaagatctc atcatttgct ttttcttcgt tttttaattt
    23401 gaaacgaagc cttgtgatac aatgtgtcaa accattgata ttttccttcc cgccgacatt
    23461 ttctacaatt ttgctcgcta attcttgata ttttcccatt tctattccct ccaatttatt
    23521 agtggaaggt ttagccaact gaatgtcact acccttattt catgcgttct tgttaattta
    23581 tcctcctttt tttatttatt tacaacgatt ctcatgcact aaacgcgcaa tatggatcgt
    23641 taaatacatt ttttcgtccg tggacatctg gtagttgaat atctcggcta aaaagtctgc
    23701 tattttctca acgcaaccaa acgtatcctt atattttttt tgaataacct ggtacaattc
    23761 ctcagaaact tctcccactt ctgatttttc tccatctagg acgcgtgtca gaaaaaaacg
    23821 taggtgtgtg acgaatcgaa aaaaatggac tgaatcttca tcaaatgtaa tcttatagta
    23881 atagcggacg atatttagaa tatcctgtat tgttgtagta aacaggtagg tattgtcttg
    23941 ttggctttga atgccttgtg cattgactaa atgcaatgcg ataaatccgg cttcatcttc
    24001 tgataacttg atgtggtatt tatccgagat tttcttcact gtttttttcc cgatggcata
    24061 ctctttagga aatagttgtt tgatatcata cagcaataca tttggtacaa atattcccat
    24121 tttgtgacgt tcgatagccg tatacaaatg atccgtcaat gcaatataca ccgaatcatt
    24181 caactggact tgtagagcct cttttgtata ttcaatgatt tcacaagaaa tttctaagta
    24241 ctcaagagga atcgcttgtg ccaattcttg gaactgttgt gaaagtccgt catttgtcaa
    24301 ctgaaatttt ttgtcgatgc atgagtcaaa aatcgggtca cccactttct ttttgaatgc
    24361 caaacctttt cccatcacga tcatttcctg atttctttca tccctggtta ttacgacgtt
    24421 gttattcagt actttctcaa tcagcatgtt ttactccttt ccatgatata tagggctgta
    24481 tccaaaacaa aaagaaaaac ccaaaaagcc gtccatggaa ccatgggaga ctcatcaggt
    24541 ttagcttgca tcaggcaatc actatccaag ttgtatcgtt ttctttatat tatcgaaagc
    24601 aagcactttt gtcaacgctt tcttagcggc tattgggcac ttctgactag ctgtcgtgat
    24661 tttttttaaa tctggtaatt acgacactaa cgctcacaga tgaagaatct gggaagctct
    24721 gttttatttt cactctagcg cttaggtcaa aatgctacta tgctactgtc ccaacttcta
    24781 atatacggtg ttccagtacc agcttatcgg tgataagcca aatttatgca aaaatctgga
    24841 aagccttttt tacaattttt ttcttatcac ctaaagctga acggagccct gtcacaacca
    24901 ctttttacac accactgctt tgcagtttgt acatatctgc atacaaacca ttttgcgcta
    24961 gaagtgtttc atgattccca cgttctacta tatgaccttt gtctaaaact aaaatcaatt
    25021 ctgcatcccg aatcgtggac agccgatggg caatcgcaat cgttgtccgt ccttgtcgca
    25081 tttttgtcaa tccttcttgg atcaatcctt ctgtttcagt atcgatattt gctgtcgctt
    25141 catccaatac tagtatttta ggatctgtca cgattgttcg cgcaaaagtg atcagctgtc
    25201 gttgtccgct agaatagctg gcaccgtgtt cgatcacttt tgagtgatat ccttttggta
    25261 attcatcgat gaacttgttt gcttgaacaa actcagctgc atggcgaatc tgttcgtctg
    25321 tgatagttgg attcatcatc cggatatttc ctgcgatatc tccataaaac aagaaggcat
    25381 cttgcaagac caatcccatt ttcttacgta actcttccat tggatattct cgaatgtcat
    25441 gatcatcaat caagatctgg ccttcataaa attcgtaaaa ccgcatcaat acgttgatga
    25501 tcgagctttt tccgcttccg gtgtgcccca ctaatgcaac ggtctctccc ggattagcga
    25561 caaaagagat attgttcaat acattgtgct ctccatcata agaaaagctt acattgcgaa
    25621 actcgatctt acctgaagta atcgcttgat ctgctccagg attttgttgc ggtgcatatt
    25681 cttctgtatc catgattttc aaaatacgac taccggcaac cattccatct gtaaaggtgc
    25741 tcaaaaagtc catcatttga gacattggat taaaaaatcc ctggacatag gtcgtaaatg
    25801 catagatcag tccaacttct acgggagaat gcagcgcatc gatcccaaac aaagtcaacg
    25861 ccaacgcaat cgccaatgca tatagaaaat tgatgatcgg acttaacaga atcgaatttg
    25921 ttttgatcat cgcatagcgc gtgtctaaat actcgttgtt cacttgttca aattctttag
    25981 ctaaacgagc ttcttggcga aactgctgga taatacgcat gccggaaatc gattcattca
    26041 atttcgtgtt cagctgactt aatttttccc gcatattccg gtaaatacga gaactgaatt
    26101 tctggtaata ccagatgacg attcctaaaa tcggcaagaa caccaaagtc cacaaggcaa
    26161 tttctccatt gatttgaaac atggcaatga aggaagaaac gactgcaaag atcccagtca
    26221 ataccatcaa aaagacatac caaaattcaa atagcgtttc tgtgtcattt gtcactcgag
    26281 aaacagtcga tcctgccggt gtctgatcaa aataacgcat acctagtgta tgcagctttt
    26341 caaataatct tacccgaaca tactgatagg tcttcaatga agccatggaa tacaaatacc
    26401 attggaaaaa ccaaatgata cttttgacta tgactccaaa caagtacata ccagcaaaaa
    26461 acaaaataac ttgtgtcgta gccgactgtt tagccagatg atcatccata aaagtttgga
    26521 tgatccttgg caataggaca ttgatcacag acagggcaaa cgcaaataag atagcaacga
    26581 tgaacgttct tctaaaaggt ttggcgaact tcatcagtcg tttgatgatc ttcttctgtt
    26641 ctttaagtgg aatcgatttt gtccattccg attgataatt ttcatccatc aggcctcgcc
    26701 tccttctatc ttcgcttcta atttctgttt ctcccacatt cgttcatacc agccgcctaa
    26761 tgcaagcagc tcatgatggg ttcctcgttc aatgattttc ccttgatcga caacaatgat
    26821 ttcttttgca tgcatcacgc tgcttaaacg gtgagctgca atgatggttg ttttctcttt
    26881 tcttatttct tttaaattat gcaagatagc ttcttctgtt ttggcatcta ctgcagaaag
    26941 tgcatcatct aagatcagca attcaggatt tttgatcaat gctcgagcaa tcgaaatcct
    27001 ttgtttctgt ccgccagata aagagactcc acgttctcca actaaggtat cataaccttt
    27061 tggtaattcc ttgatttcat tgttgataaa tgctaaagaa gccgcatgct ctacttcttc
    27121 ttctgaaagt tttggattag aaaaacggat attatctcga atggtcatcg aaaacaagaa
    27181 gtgatcttga ggcacatagc cgattgatcc taataaagca ttcaatgaat attctttgat
    27241 cgaacatccg ccaaattgaa tttttccttg ataatcatcg tattcacgca tcagtaattt
    27301 gatgattgtc gtttttcccg caccagtttt cccaacaata cctaatgttt ctccttcttg
    27361 gatcgcaaat ttgatttgtt ctaatgctgg tgattcttct ttcggatagt taaaagagga
    27421 aatagctaca ttcagctcgc cttttgccgg agtctgcact gcacccagtt gttccacgat
    27481 atgcgtcttt tcatgcaaaa gttcattgac gcgatcataa gaagcatttc ctctttccaa
    27541 tacgttgaac aatcgaccaa tcgcaaacat cggccaaaca agcataccga tatagctaat
    27601 aaatgagacc atctgcccga ttgagatcgt gccttccaag acatactttc cacctaaaat
    27661 aattgttgca acataagaca gaccgataat caaggtaatg aatggatcaa ataaggagtc
    27721 taaaaaattg actcgtttgt ttttaataat tgcatcttca atcttttctt caaaatccgc
    27781 gagatcttct ttttcttgtc caaatgtttt aatgactttg atcccagtaa tactttcttg
    27841 tgtcttatca ttgatactag aaaaagcttc ttgcgaatca cgaaaagcat cgtggagttt
    27901 tgaccccagc actcgtgaag taacggctag taaaggcaga ggaatcagcg caatcagtgt
    27961 taagcgccaa tcaataaaca aaatcatcgc aatgatcgtt gttccgcccg tgatcactga
    28021 atctgcaaag gtcaagatac cagctcctgc tacattttgg attgcattca aatcattcgt
    28081 tgcatgagcc atcaaatctc cagtccgata tttttggtaa aagagattgt ccatttctgt
    28141 aaaatgtttg aatagctgct gccgaagttc tttctccaac cgagctgcac ttccccaaat
    28201 attcgtccgc cagatataac gaaaaagata ttgtaagaga gctgcaagga tcaataataa
    28261 gatccaccag gaaatgatct ttattgataa atttttatcg gcgatctcat caatgatgat
    28321 cccaatcact tttggaggag ctaactgtac caaggccact gcgaataggg agaaaatccc
    28381 aattaaataa tgtttctttt cctgtcgaaa aaaccaacct aattttctaa aaatcgacat
    28441 tttactcctc ctttgtcttt atcaagagca taggattgtc tgtgcttgtc ttttttgctg
    28501 aatagtctga atagtctaac aaattacgaa ataaaaaacg gactgaaaaa atcagcccgc
    28561 aaaaagtctt tgggtcagaa cctatttttt cccttggttc ttcatttgct gcatcatctg
    28621 gcgaattttc ttctcagatg gtttttgccc cattgacatc atcattgatc gcaacatgtc
    28681 ctcattaacg ggaggatttt tttctaaata gtctttcata tatttacgcg ctaggaaaaa
    28741 gccacctact gcgccgatta gcaaagcaat aatagcaata agtacgacaa tacctgttga
    28801 taccatataa atctctcctt tctatttccg aattgctctc aatgatttat tttactaaag
    28861 tttattgaaa aaagaaaggc gtattcttta gtttcatgca aaaaatagtg gtttttttag
    28921 agaagaacaa aatttctttc attaaccagc aagacacgcc acgaaaaaaa attaggcata
    28981 ccaaatcttt ttctttaatt ttttctttct gaggtgtaga atgtaatcgt agataaatag
    29041 gaaaggtggt tttttcttga ataatcaatc caacaaacaa tttcataagc atcgacttat
    29101 tgaacataca aatggtttat ctttagctga agtaaatgga acagtggatg tacccaaggg
    29161 aaaaagtttt tttaaaacac ttcttgctta ctctggacca ggtgcattag tagcagttgg
    29221 ttatatggat ccaggtaact ggtccacttc gatcactggt ggacaaaatt tccaatattt
    29281 attgatgtca gttattttga tgtctagtct gattgctatg cttttacaat atatggcagc
    29341 aaaattagga atcgtgaccc aaatggattt ggcccaagca atccgagcac ggacaagtaa
    29401 gacattgggt gtcattttat ggatcttgac tgaattagcc attatggcaa cagacatcgc
    29461 agaagtcatt ggtgcagcaa tcgccctcta cctgcttttt gatattccat tggtaattgc
    29521 cgtatttatc actgtgttag atgttttcgt ccttttattg ctgacaaaag tcgggtttcg
    29581 taaaatcgaa gctttggttg tctgcttgat tttcgtcatt ttattcgttt tcgtctatca
    29641 agttggcttg tctaaccctg attggcacag tttgtttgcc ggatttatcc ctaacagtca
    29701 cacattctca gactcaccgg tcatcggcgg cgaaactccg ctgactggag ctttagggat
    29761 tattggtgca acagtcatgc cgcataactt atatcttcat tcagcagttt ctcaaactcg
    29821 gaagatcgat caccaagacg aagaagatgt agcacaagcg gtacgctttt ctacttggga
    29881 ttctaatatc cagttgtcaa tggcttttct agtcaatgca ctgctattga tcatgggtgt
    29941 ggctgtattc aaaaccggtt ctgtccaaga tccttccttt tttggtctgt atgaagcttt
    30001 atctgatcca tcagctatga gcaatggtat cttgattgct gtagcaaaat ctggtatctt
    30061 gtctacttta tttgctgtag cacttcttgc ttctggccag aactctacta ttactggtac
    30121 gttgaccggt caagtcatca tggaaggctt catccatatg cgtatcccaa tttggctaag
    30181 aagactagtc acacgattgc tttctgtgat tcctgtattg atttgtgttt tgtttacaag
    30241 tacaaaagga acgatcgaag aacataccgc tttgaataac ttgatgaatg aatcccaagt
    30301 ttttctggct tttgcattgc cattctcgat gattccttta ctgatgatga ccaatagcca
    30361 agcggagatg gggagccgat tcaaaaattc atttgttgta aaactacttg gctggttctc
    30421 tgtgattagt ctgacttact tgaatttacg aggattacca ggtcaaatcg aaagtttctt
    30481 cggcgagcaa gccacctcag ctcaactagc attggctgat caaatcgctt atgtaatcat
    30541 tgttcttgtc cttctcctgt tggcatggac gattggtgat ttatataaag gaaacaaaaa
    30601 atatgaacag cgtttgcagg aaacgataga agaaatcgag tagaaactga ttcttttaca
    30661 caaaggaggt tgtgtcagaa gtgggcagct tcaagaaata agaaggaatt taagaaaatt
    30721 gcttttcaaa tttttgtgaa atttcggctt atttccgaag aagttacttc tggaacaccg
    30781 tgtatcagag gttgtgaaaa agctgccttg catcaagtaa taagaacagc caagtaaaaa
    30841 cagcttttta tgtttttaac ttggttggac ttattaccgc agatgcaagc ttttgagcac
    30901 cgtttattcg gaaaaaggaa ctgcgacaag acttttgtca cagttccctt tcctacgatt
    30961 agtcaagtaa aaggtgtaag aaaaatagct cataaacgaa agcgacaaaa tcaa
//
LOCUS       DACJGB010000030        29376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.030, whole genome shotgun sequence.
ACCESSION   DACJGB010000030 DACJGB010000000
VERSION     DACJGB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.030"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(182..1480)
                     /gene="asnS"
                     /locus_tag="IYI34_001395"
     CDS             complement(182..1480)
                     /gene="asnS"
                     /locus_tag="IYI34_001395"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAR0559269.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(1520..2710)
                     /locus_tag="IYI34_001396"
     CDS             complement(1520..2710)
                     /locus_tag="IYI34_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR0559270.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(2731..3258)
                     /locus_tag="IYI34_001397"
     CDS             complement(2731..3258)
                     /locus_tag="IYI34_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAR0559271.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(3305..6094)
                     /locus_tag="IYI34_001398"
     CDS             complement(3305..6094)
                     /locus_tag="IYI34_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAR0559272.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(6241..6441)
                     /locus_tag="IYI34_001399"
     CDS             complement(6241..6441)
                     /locus_tag="IYI34_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR0559273.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(6894..7214)
                     /locus_tag="IYI34_001400"
     CDS             complement(6894..7214)
                     /locus_tag="IYI34_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559274.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(7717..8835)
                     /locus_tag="IYI34_001401"
     CDS             complement(7717..8835)
                     /locus_tag="IYI34_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559275.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            9306..10613
                     /locus_tag="IYI34_001402"
     CDS             9306..10613
                     /locus_tag="IYI34_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAR0559276.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            10734..11933
                     /locus_tag="IYI34_001403"
     CDS             10734..11933
                     /locus_tag="IYI34_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAR0559277.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(11960..13228)
                     /locus_tag="IYI34_001404"
     CDS             complement(11960..13228)
                     /locus_tag="IYI34_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAR0559278.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(13398..14039)
                     /locus_tag="IYI34_001405"
     CDS             complement(13398..14039)
                     /locus_tag="IYI34_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAR0559279.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(14448..14951)
                     /locus_tag="IYI34_001406"
     CDS             complement(14448..14951)
                     /locus_tag="IYI34_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR0559280.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(15074..15838)
                     /locus_tag="IYI34_001407"
     CDS             complement(15074..15838)
                     /locus_tag="IYI34_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAR0559281.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            15946..16221
                     /locus_tag="IYI34_001408"
     CDS             15946..16221
                     /locus_tag="IYI34_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAR0559282.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            16781..17731
                     /gene="yidC"
                     /locus_tag="IYI34_001409"
     CDS             16781..17731
                     /gene="yidC"
                     /locus_tag="IYI34_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAR0559283.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(17777..18178)
                     /locus_tag="IYI34_001410"
     CDS             complement(17777..18178)
                     /locus_tag="IYI34_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAR0559284.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(18192..19001)
                     /locus_tag="IYI34_001411"
     CDS             complement(18192..19001)
                     /locus_tag="IYI34_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR0559285.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(18988..19878)
                     /locus_tag="IYI34_001412"
     CDS             complement(18988..19878)
                     /locus_tag="IYI34_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0559286.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(19891..20370)
                     /locus_tag="IYI34_001413"
                     /pseudo
     CDS             complement(19891..20370)
                     /locus_tag="IYI34_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(20546..21487)
                     /locus_tag="IYI34_001414"
     CDS             complement(20546..21487)
                     /locus_tag="IYI34_001414"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAR0559287.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(21484..22479)
                     /gene="lacD"
                     /locus_tag="IYI34_001415"
     CDS             complement(21484..22479)
                     /gene="lacD"
                     /locus_tag="IYI34_001415"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAR0559288.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(22472..23668)
                     /locus_tag="IYI34_001416"
     CDS             complement(22472..23668)
                     /locus_tag="IYI34_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR0559289.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(23686..24414)
                     /locus_tag="IYI34_001417"
     CDS             complement(23686..24414)
                     /locus_tag="IYI34_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0559290.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(24855..25829)
                     /gene="lacD"
                     /locus_tag="IYI34_001418"
     CDS             complement(24855..25829)
                     /gene="lacD"
                     /locus_tag="IYI34_001418"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAR0559291.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(25826..26284)
                     /locus_tag="IYI34_001419"
     CDS             complement(25826..26284)
                     /locus_tag="IYI34_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAR0559292.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(26281..27744)
                     /locus_tag="IYI34_001420"
     CDS             complement(26281..27744)
                     /locus_tag="IYI34_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0559293.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(27726..28658)
                     /gene="pfkB"
                     /locus_tag="IYI34_001421"
     CDS             complement(27726..28658)
                     /gene="pfkB"
                     /locus_tag="IYI34_001421"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAR0559294.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            28800..>29376
                     /locus_tag="IYI34_001422"
     CDS             28800..>29376
                     /locus_tag="IYI34_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR0559295.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         8595 a         6323 c         5044 g         9414 t
ORIGIN      
        1 gccatttcca caaaattttt cttaatactc gaaactaaac agctttttca caacctctcc
       61 actaaacggt attcaaaagc ttacatctgc ggtaataagt cccgccaatt caaaaacatg
      121 aacagctgtt tttgcttggc tgttctaatt acttgatgca gaacagcttt ttcatcacct
      181 cttatggata aattcggttc aacaaacgtg ggaatggaat agcttcacgt acatggtcga
      241 taccagcaat ccaagtgact gttcgttcaa ggcctagacc aaaaccagag tgcggaacag
      301 aaccgtattt acgtagatca agataccatg aatattcttt ttcatccaaa ccgtgattac
      361 ggatttgttc tagcaagaaa tcatagtctg ttgcccgttc gcttccgcca ataatttcac
      421 cgtaaccttc tggtgcgatc atatctgcac aaattaccac gtcttctctt gttggatgtg
      481 gtttcatgta gaatggcttg atggcttttg gatagtttag gataaagaca ggctgttcga
      541 atgaattcgc aatgaacgtt tcatgaggtg aaccaaagtc ttcgccccat tcgatgtcat
      601 caaatccatt tttcttcagt aattcgattg catcatcgta agtaatacga gggaatggca
      661 attttgtata agtttttaag acttctttgt ctcgtcctag tacatctaat gcatattcgc
      721 aatgatctaa aacattttgt actaagaatg ctacatattg ttcctgtact tctaagcttt
      781 cttcttgatg agtgaaagcc atttctggtt cgatcatcca gaattcgatc aaatgacgtc
      841 gtgtttttga tttttcagca cggaaagtcg gaccgaatga aaagactttt ccgaaagcca
      901 tagcagcagc ttccatgtat aattggccac tttgagataa ataagctttt tgatcaaagt
      961 aatttgtttc gaatagatcc gtagtgcctt ctggtgcaga accagtcaag atcggcggat
     1021 cgattttgac aaaatgatgt tgattaaaga attcatacgt tgcacgaatg atttcatttc
     1081 tgatctgcat aatcgcatgt tgtcttgaag aacgcagcca taaatgacga tgatccatca
     1141 agaaatctgt tccgtgttct tttggtgtga tcggataatc gtggctctcg cttaccactt
     1201 ccacatttgt aattccgatt tcataaccaa attttgaacg actgtcttca cggatttctc
     1261 cagttactaa cacggatgtt tcttggtgta gatttttagc tgtttggaaa acttcttccg
     1321 acacttcgct ttttacaact acaccttgga aaaaggctgt tccgtctctt aattgtaaaa
     1381 atgcgatttt tccgcttgat cgcttatttg cgacccaagc gccgattgtt actgtttctc
     1441 ccacatggtt tttcgaatcg atgatttgaa tttgttccac gtatgactct ccttttatat
     1501 tccttgaatt tcttcaggat taattttgac ttttactctc aataaattgc ttgattctgc
     1561 gtaccgcttc ttctaacgta gcgcgatcag tggcataact catgcggaca ttttctggtg
     1621 ccccaaagcc ttcgccagtc acaagcgcta cccctgtttc ttctaaaaga tcttctaccc
     1681 attgtgtgac attttcatac ttacacatgc gtagcgtttc ttttacatta ggaaaaagat
     1741 agaaagctcc ttgcggcttt tcaagagaaa cacccggcag ttctgccaca agcggatata
     1801 acgtattcag gcgttcttca aaggcgttcc gcatttcttc tactgtatct tgctcacctt
     1861 tcaatgcttc aacagctgca tattgactga ctgctgtcgg attgctagtt gattgggaag
     1921 caacagcgat cattccattg atgatcacag gatttcccac cgcataacca atgcgccagc
     1981 ctgtcatcgc atatgtttta gacacgccgt tgatgatgat ggtctgattt ttgattgctt
     2041 ctgaaatcgt tgcaattgga gtaaactcat tgccattata aactaaacgg ccataaatat
     2101 catcagcaac tatcaaaata tcatttttta ctgcccattc accgattgcc tgcagttcat
     2161 tcttgctata aatcattcca gtcggattcg atggagaatt gatgattacg gcttttgttt
     2221 tttctgaacg tgcttgttct agctgttcaa cagtaacttt ccagctattg gattcttccc
     2281 ctttcacaaa gacaggaagt ccttctgcca gttttacttg ctcaccgtaa ctgacccagt
     2341 aaggcacagg aatgatgact tcatcttgcg ggtcaaggat cgtttggaaa agggtataca
     2401 aagcaaattt cgctccgtct gttacgactg tttgtgaagg ttcatagaca agtccatagt
     2461 ttttcttcag ataatcaacg attgcctgtc gaagctctgg gattcctgct gttggtgtat
     2521 aatagctagc tttcccattg cggattgctt cgattgctgc ttcttggatg ttttctggtg
     2581 tcgcaaaatc cggttctccc acagtcaaac tcagcacatc tctgcctttt gcttttaatg
     2641 ccttggcctt tgccgcagct gccaaagtga cagacggttc taatcgtttt gttctttttg
     2701 ataactccat cccattcatt cctttacctt ttaaattccc ttgattgtct tgacttcatc
     2761 tccagattca aagccaatca ggtaataatt cagtttccca tttgtttctt ttcctactac
     2821 ttcccacaca ggctgatcat catacatccc taaagcagct ttttcgattt ggatttcagg
     2881 atgggccgca gcaattgtct gttttgcttc gttttcagtc agtccctttg tttgatccaa
     2941 tacttttact ttttcaccag atttaggaat gataacggcg atttcttttc ctttatcatt
     3001 tgtacctgtc agtgaaaagt atgtcttctt tcgagtaaac caataaaaat gatcggtttc
     3061 ctcaagattt gcatatttct ttgcgatttc tactgcttct ttcttcgcct gcaccgccgg
     3121 acgactggaa cgcaagtaaa aaatcgagct gatcacgatg accgctaata aaaatacgat
     3181 caagccaagt aacacgcgat taatcgtttt ttcctttgtc tgttttttct tcaagggtcg
     3241 tcgctccttt atcttcattc cttttcatta tagcagattt taattatgaa aaaagacagc
     3301 tcctctaact tccttcttca tttttcttta agaattcatg gatatcctga tagatttcct
     3361 ccatagctag ctctttgatc ggtacttttt tcggtaatgc atggatgatc cgtgcgccat
     3421 attttgcagt gactagtctt cgatccaaaa taagcaatac gccacgatct gtctccgtcc
     3481 ttatcaagcg acctaatgct tggcgtaact tcaaagctgc tttaggtacg gattcctggt
     3541 aaaaaggatt gatcccctct gattctaaat aagcgttcct agctttgacc atcgggcgaa
     3601 gcggattatc gaagggtaaa cgtgtgacta caacgatctg caaagcatct ccaggcaaat
     3661 ctacaccttc ccaaaagctg tcagcgccaa acaatacgct tttttctgac aaaatgaagc
     3721 gtttcaaaag cttctcccgg cttccaccaa acccttgagc tagtatttct cgtccttttt
     3781 ctagcataga cagatgcatt ttctgataga ccttctgcaa aacatcatga gaagtgaata
     3841 acacaagtat tggccgatct tcttgttcga tcagacctgt aagtgctgtc tctaaataat
     3901 cgatatatgc ttgattgctg acttgatgga cggcaggtgc ctcttttggc acatagatcc
     3961 ttgcctgttt ctcataatca taaggagagg caatcacttt aagaggtgct gcttcaatgc
     4021 caagcctctt agagagatag tgtcgattag ggccgatctt cagcgttcct ccaatataaa
     4081 gaatacgatc atatcgagga taccaaacag tcgacggcaa catagccgct tgaaaatcat
     4141 ggagatgaaa aatcatctgc tgcgctgtgt tcccaggaac gaaccaatgg atcaaatgat
     4201 ctcgccagtc ttccagccat tgatccatca gcaagtattg cttgtctaat tgatcaaata
     4261 ggctatataa acttgctaaa tcgatccgca tctggtttgt ccagctttct tggataatct
     4321 caaaagtcag acgcatttgt gactgtagct gctggatctc ctgataaaaa agctgcaggt
     4381 gatcaatggc tgtcccagat tccagtggca gctgatccaa catctctttt gtcatcatga
     4441 cttcttctgg atactgtccg ttaggttgct tgccgctcac taaactcatt aatccttctg
     4501 ccatacaaat ctgtgcttcg catagcgcag aaagttcttc tcgataaatc gataaccagc
     4561 gtaaggcttc atgttctttc ggtaaatagc tttgtattcg gtcaaacagc tgatttggtt
     4621 cttctaattg ctggatttgt ttatggaaat agttaatact taatcgtttg tcatttactt
     4681 tttcagcgat ttccggcaaa tgatgtactt catcgatcaa caaaaatgga ctagctggaa
     4741 gcaatggttc ttttcgctgt gtttcttgta ctaaaaacgc atgattgaca atcaaaaaat
     4801 tactttgcct gattttcttt tgcaagaaac gaacaaagtc ttgttcgtaa aatgactgat
     4861 ttcttgctaa aaatccaata cctctgtgcg cgatatctgc aaagaatgaa tggttcaaat
     4921 ttgttaaatg taattcatcc aaatcaccag tcttcgtttg cgtcagccaa actaagaccg
     4981 ccatttgata caaagcatat tgtttttgtt ctggcggttc cacaagtgtg cctttaaaac
     5041 gctgcaaatc aatatagtgg cgatagcttt tgataattac aggttgtatg ggctgatcta
     5101 gcaattgatt cagcaatgga atgtcttttt ctatgatctg ctgctgcaaa actagcgaaa
     5161 cggtactgat gacagcgggt ttttcaggtg tagccaaata actcaatggc agcagataac
     5221 cgattgtttt acccattcct gtagctgctt cgatcatcaa atcttttgat tcatcttttg
     5281 tgaaatggtc gtagaccgca ttcatcagtc tgttctgttc ttttcgatac atcagtgttt
     5341 taccaaacat tttttctttt gcttgttttt ttcttggata tgtccgctcg ccataatgga
     5401 cggatgtgaa tagttccact tcttttttct gcaaagctag cccatcgacg atttctaatg
     5461 agggatcaag tggctctggt ttttctttca tttcttcaat cacatgataa atgaaacgac
     5521 tcgtatccat acctgtgtgt ctacttaatc gcgcaatctg ctctaacgtg atgattggca
     5581 attttctcat ccgttcttcg atcagcaaaa gaagttgacc tgtcacttcg gcatcactat
     5641 ctgcctgatg aggattctca tgaataaatc caaggctttc tgacaaatca gctaagcgaa
     5701 agcttggttc cgtcggcaag aaaatttgtg caagttctac tgtatcgatg cctggtatct
     5761 tcaaactagg aagtccgcat cgcatgagtt catggttcaa aaaattataa tcaaaataaa
     5821 tattatgcgc gacaaatatg gtgtctgcca aaagattgta aatcgtttgg gcgacatctt
     5881 cgaaataagg cgctttttgg acgcgttgat tcgtgatccc tgttaaatgc tggatttgtt
     5941 ttgaaatctt ccgccctgga tgaatatcga tcgaaaaacg tgaagtgatc cgacctgatt
     6001 caatcatgac gcagccaaat tggatgatcc gatcttcttt tggattcgtc ccagtcgttt
     6061 cgatatcaac gactgcataa gttcttggtt tcaaaatatc ttcctcttct cctgcctcta
     6121 atgttctttt gaaattatac tacaaagtgc agaaaaatac tctcatcgct ttttaaagaa
     6181 aatagacaaa aacgcccaga cacgcaaatg tctgggcgtt tattttttag tataggttaa
     6241 ttaagcttta gtaacgtttg ttgcttgagg tccgcgttgg ccttcttcaa catcgaaagt
     6301 tactgcttgg ccttcttcta aagttttgaa tccgtcgcct tggatagctg agaaatgaac
     6361 gaatacatcg tttccgtttt ctgcagtgat gaatccaaat cctttttctg agttaaacca
     6421 ttttactgta cctgtttcca taataaaaat cctccttgtg tatcaacaca tattttgcaa
     6481 ttacttgaat ggtgtatatc tggaagatgt ttatttgaaa caagctacca cagattaccg
     6541 aacaaaaact actcctatag tatatcatac cgtttacaaa aaagctagag tttttcgact
     6601 ttttttatta aaaaaagttt tctcagcgat tacataaagt gaaaaaccga actttttttt
     6661 aaaataaacg acatattgtt cagttggtgt ttttttgctc tgatgttctt ttttaaacta
     6721 aaaaaattat tctttctgac tacttatcgg ttctgtttca acggtcgagg ctggggaaga
     6781 ttctttagta ggtctgatgt tctcatggta tttctgatag cctatgacag ttaccagaag
     6841 aacaaggcaa accaatacga ccaacgagat tagtttttct tttttgctca tatctatacc
     6901 tcataaataa caggatgatg tccatcatat ttctgagtat gagtaaaagc aaaagctgca
     6961 gctgtagaat gtaagaaatg ccagcaactt tcataatcac aggtcggaca gtaccaaaag
     7021 taaccaaatc ttggctgcaa agaccgttct tctgtttcag gaatcgcaga atcaagatct
     7081 ggtgtctgta cataaacatt tttgtacaga cgtgtgcctg taataggaaa ccgctgtgtt
     7141 tcttcagcaa aaactggctg acttgttaaa cttagggata gagacagcaa acaccataac
     7201 agtctttttt tcataaagac ctccttatcg ataagttttt cccaattaaa atcatatctc
     7261 aagaacgccc gttattcaca ttttgttcat aaatgatgtt ttttgtatca taatcgtcaa
     7321 aaagaggtcg tgaccacgtc acaacctctt ttttaaacgg tgttccagaa gtagcttctt
     7381 cggaaataag ccgaaatttc acaaaaattt gagaaataat ttttgtcaat ctcttcttat
     7441 ttcttgaagc tgaccacttc tgtcacaacc tcttccttaa acggtgttca aaagcttgca
     7501 tctgcggtaa taagcccacc aaacaaaaaa tatgaagagc tatttttgct tgctgttctt
     7561 attacttgat gcagaacagc tttttcacaa cctctcatac acggtgttta atcagctgac
     7621 cttcggtatt agctcaaaaa acggcaaaat atgtgaaact atttttccgt ttgtttctct
     7681 aatactcagg tcaaaacact gatttcacaa cctcttttaa taacgaagaa tcgctgtcag
     7741 actcttttca tctggcgcat ctttttgatc caaaataaac acttttccgc cattaatgat
     7801 cacatcatct gctaatgcat tcaacacttg acgccgatca tattctgttt caggatcttc
     7861 accgaaacca tcagctaagt ttgacgtagc gataaataaa tgtgacactt ttccttcttt
     7921 cgcagctgga acgatatcaa ctaactgatc gatgaatttc ttatctaaga gtttattgta
     7981 ttcttttgat tcttgagctg cgagttcact tgacagcttc ttcgcccctt cttcgatatc
     8041 tctaaaggat agctgcgcag gagaagcaga aatcgcaacg tctgcacaat aatagggatt
     8101 tttagcaatt tttttgaaca tcgtctgatt ttctggtaga gcaaataaat aaatcggcca
     8161 ttgttctgga ttttcgaaag aatccttcaa gtacgtatca actgcttggt aatagttgac
     8221 ccaatcgatc tctacttctt catcttttgg atttacgcca tggaaagtta ctccatctcg
     8281 ggctccgcct cctccattag aagagaagtt caaaccgcca cctgtcagtt cagatcccaa
     8341 tgcagtaacg acatcaaccg gtgccccttc tggtagagat acttctttta cttgtttgtt
     8401 gctgacttca tacaatttca tggagtcgcg gttcaatccc aataaataat aagaatagtt
     8461 gaactgccca tttttgataa tagccagtaa ataaggaaga tcacttacat agtattgatc
     8521 gtctaccggg atactcaaac gatggatata ggtttggtca gcagttataa tgaccgctac
     8581 actcgtagtt gcactacgcc aaaacgaagg atctcctaac aaattttcaa ttttttcttg
     8641 gaacggggac cagtcatgtt cggaatattt tttctcaaaa cgtttctttg cttctttcgc
     8701 aaaatttttg aatttgatct gatctttttc cacatcttga tgggcgacat gcgtattcag
     8761 catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc aattgtgaca ataattcttt
     8821 atcctttttt gccataaacg tacccctcct aaaaaaatca ttcctttgtt tctatctaaa
     8881 gtctaccaca acactatttt tccatacaat aaaacgtatc taaaaaaata aaacggtttc
     8941 tacggtttta aatttagcta tttttttaag ttatcttcat aatttcctca attatttagc
     9001 cccagtcttc aactattctt catgaaatgc tgataaacta agaaaaagaa taaaagaggt
     9061 ggatcttatg catttgatta tgattttcag tttattaagt attatcagtt tatttgcttt
     9121 cagcgcttcc cgcttattag atgcggaaac acagcctatt cctgtaaaag tacgtaaaaa
     9181 cgaccgctaa ccttccataa cgtgcgttct tatcctaaag ttttttcaga aacattgaaa
     9241 ctctctcccc tctctgataa aattttaatt tgtagcgaaa caaattaatt acaaggagat
     9301 cggatatgtc cataaaaaaa acacgtctag tcctattatt ggctagtctc attctttgcc
     9361 ttgcttcttt tctaccagct gttgctgtgc aagctgaaga tactttcaaa gtaaatgcaa
     9421 aagcagcctt tgctgtagat gcgcaaagcg gtaagattct ttatgatcaa gatggagaaa
     9481 aaaccatggg gatcgcttca atcacaaaaa tcatcggtct ttacatcgtc cttgatcaag
     9541 taaaagaagg gaaactatca tgggatgata aagtttcaat ttcagattat gcagaaaatt
     9601 tgagtgtcac cccagattta tcaaatgttc ctttgcataa agaaaacacc tacaccgtta
     9661 aagaattatt cgactctgca atcatccagt cagccaacgc ttcaatggtt gccttggcag
     9721 aaaaaatctc tggcagcgaa gcaaaattca ctgaacgaat gaaagaacaa ttgaaagatt
     9781 ggggtatcaa agatgcaacg atcgttaatg catcaggact gaataactct tatcttggcg
     9841 aaaaccgacc agaaggtact ggagaaaacg acgaaaatca aatgtctgct caagatgtgg
     9901 ctatcgttgc ccgtcatttg atcttggatt ttccagaaat actggatgta tctagcacta
     9961 cgacacaaat gtttggcgaa aacacgcaat caccagtaga aatggtcaac tggaactgga
    10021 tgctcccagg attcgtgaat tataaagaag gcgtggacgg attaaaaact ggtacgactg
    10081 aacttgcagg agcctgtttc gtcggtacga tcactaaaaa cgatcaacga atcatcactg
    10141 tcgtattgaa tgcagacggc catgcggaaa acccaagtgc ccgtttcaat gaaacagctc
    10201 gcttaatgga ttattgctac gacaattggt cagaaaaaga gcttggaaaa gccaatgcaa
    10261 gtatccctag tctaaaaacc attgatgtaa aagacggaaa agaatcaaca gtgcccgttg
    10321 tgcttaaaag tcctgtaaaa gtatgggtac ggaacgatat ggatactgga aaattaacaa
    10381 tcacaccaac tcttgacaaa aaacaagtca ctgacaatga gatccaagca cctatggata
    10441 aaggaacaaa aatcggtacc gcaacgatca ccttggcaga agataaatta ggttatcttg
    10501 aagacaacaa cgcgccatcc acagaaatca tcacggataa aaccgtagaa aaagcaaata
    10561 ttttcgttat tggctggcga cgcgtcgcag aatttttcgg taacttgttt taattgaaca
    10621 aaaagaaaaa agcatctttc catgtggaag atgttttttt gctttttcta gtttaaataa
    10681 cagctttttt catctatttt cgttatactg tataagaaaa aggagttgaa tttatgaatc
    10741 taaccagaga tctgattttt ttggttctct atcttattgg ggtattctcc atcgtttatt
    10801 ggcgaaaaca gaaaaaggat ccttccttgt tcttttacag cagtacatgg acgtttgcgg
    10861 taatcttgac ttattttgtc gtgttggaaa ttttttattc gaccttttat tttttgattc
    10921 ccttagtgtt tttcagtatc tttgcctttt cctatttttc agaaaagcga aggctgttga
    10981 atggtctttt attcaatatt ttcctcatta gttttggtat ctacttgttt gtcttgctat
    11041 acgaaacaca aaatattttt cttggcggac tgattgcctt gatcacgatc ccgttactgc
    11101 ttgtctttct attcggcatt tatggattga tcgtttttct tttttggaac ggtgtcacag
    11161 ttttacgtag agagtcgcat tcattagcta atcttttaac gttgatacta gccatttttc
    11221 ttacattgtt tcttgttttt gactttttct tgttaaaata tttaccacaa tggataaatg
    11281 ctttgttttt ctgcatccca ttgatcttga tctatctatt tatcgtattc tacaactttt
    11341 tgactgtctc attcttgtat cagttcaacc gccctcgata caaccaagac ttcatcgttg
    11401 tgttaggcgc cgggttgatc aatggagaaa cagtttctcc tcttcttgcc aagcgaatca
    11461 ataaggcgat tgctttttat tgggcacaaa gccgtgcgac attaaatccg cctatcctat
    11521 taatgtcggg tggacaggga gcagacgaaa aagtaccaga agcaatggcg atgaaacaat
    11581 atgccatgga acaaggaatt cctgaaagag atattcttgt cgaaaccaat tcgacaacca
    11641 ctttggagaa tatgcgctat tcaaaggaaa tcatggatca gcaaatgaaa ggtccctatc
    11701 gcgcgatctt cagttccaac aattaccaca ttttcagagc tggtctctat gcacgacaag
    11761 cgaaactgaa ggcaaatggc attggggcaa aaacagcttt ctattacttg cctaacgcgt
    11821 ttttaagaga atacatcgcc atcttgatga tgaacaaaaa aagacatatg atcatttgcg
    11881 gattgctttt cgcaggttcc gcattcttat cactgatcac tttacttttc taaaaaaaca
    11941 aggtgtgaca aaacatatgt cacaccttgt ttttttcttt tattctcttg acgattggca
    12001 agaatagtat tccttttttg acggctactt gctgtgcaga ataaataccg ctgtttcctg
    12061 aacacataaa gctgatcaag cagatcataa agaagtaagc agcagcttgt gaaccaaata
    12121 attcgattcc catcacaaaa caggcaatcg gtgtatttgt cgcaccagaa aataccccga
    12181 taaaacctaa tccagcaaga aaaggaacag aaacatggag caaaggcgct agtactgcgc
    12241 caagcgttgc accaatctca aatagtggtg tcacttctcc cccttggtac cccgcaccta
    12301 aagacaagac cgtgaagaat aatttcccaa taaaatcata agcatgtgcc tgtccattaa
    12361 atgattcctg cagtaacgga agactcaacc caaggtaacg ttgtgtttgc aaaatcaacg
    12421 ctgcggctac aactactgct ccacctatcg tatttcgcca aacaggatga ggaatccatt
    12481 gcgcataccg cgcttttaaa aagacgatcg aacggctgaa tacccaacca atcaaaccaa
    12541 aagcaatccc agcaacaaaa actttgaaga acagcaacat actccattca ggaatcacac
    12601 ccatttggta atgtagatga gaaacaccat agctttctgt catgaaattt gcaaaaagcg
    12661 cagcaaagaa gctaggaaat attgcttctg ttcggacttt accgattgcc aacacttcta
    12721 atccaaataa tgttccagct aaaggtgtgc caaatacaga actaaaaccc gcactgattc
    12781 cactgataac caaaatctcc cgttctgctt tatccaatcg gaataaatgc gctatattat
    12841 cagctaatgc tccccccatt tgaactgctg ttccttcacg accaacagat cccccaaata
    12901 aatgggtagt gatggtccca aacaaggtca atggaatgag ccgcaaaggg atcttctctt
    12961 ctcctccatt cccttgatcg atcactaagt tattcccacg actagaaaga ccgccatgat
    13021 acgcataaag ataagcaaaa agcgcgccgc tgactggcaa tagataaagt agccaaggat
    13081 gtgctagtcg gatatctgtc acgaacgtca ggctatttaa gaaaaaagca gacaagctcc
    13141 ccatgaatac tccgagaatc gctgtgacca ccagccactt caacatgtac atcaaaatac
    13201 gcactggact cggtaattgt ttttccataa aaaattcctc ctcaaataaa aaaacgccta
    13261 ctaaagaaag gggctggccc cagactttag taggcgtcat tagctatctt acgttagcat
    13321 ttaaaggcga acaccatcac ctattcactt ccattaaaag atagcatatt tttctgtaaa
    13381 aaacaataaa attatcgtta tttctttaca cgttcagcaa attcatagaa aggagccaat
    13441 ttcgttaact gttgattaca ttgatcacta tcatgacaaa tataattccc ctttttcgta
    13501 tacgtgccat cagaaccggc tttcgttgta gaaagaaaga gcgagacatt cgatgttttc
    13561 tgacagatcg cacagattcc cttattaatc gttggtgata aagtaccgct gatccctttt
    13621 aattttccat cttcgtaata aaccatgaat tttttttgcg tccctggatc attccaaccg
    13681 atgtacgtat gtgcttgcaa gtcgatttca ttccaagcgg gttgtttgag ttttttcact
    13741 tttcggaata atttctcgat ttgtggtttg ctcggctgca taaacggctg gacgatttgc
    13801 ttcagctcct ccaatgctcg ttctgcttgc gtttttgtca gttttacatc taaaactgtc
    13861 gttaaaaacg tatcgatttc tgatggttcg taacctaata catcgcgaat cttttcagct
    13921 gctagagcct gaaccgtttg taccgttttt gagtcattga cagatgagta cgcatttaat
    13981 aactgctcta cttctctttt tataaaaaaa tattgatatg gtttcattgt ttgttccatg
    14041 attttaaagt cctttcttct aaccaaagtg catactttgt agaaaaaagt gatcttctgc
    14101 aaagtatgtg ttacctgaag atcccggact cttttaaata agatacgttt tgcatcttat
    14161 catcaccccc tttaatgttc attcttctat gtacatgatt cattcgataa ataaaagtaa
    14221 accgtatctt gagcagatag tcaactgcaa acagaaaaag gactgtgaca aaagtcgtgt
    14281 caaagtccct ttctacaaac aaacggtgtt ccagaagtag cttcttcaaa aaataagccg
    14341 gaatttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
    14401 cccacttctg tcacaacctc aatatggtct attattggtt ttacctgtta aatcgaggat
    14461 tcttttccga agtaacgttg ccatctctta cgcatacttg ctgacatagg ctgagcggct
    14521 tctttgactg cgcagtattt gtctacgaat gttacgatat atccttcttt gtacttaggc
    14581 ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat ctcgctcaag atccgacaaa
    14641 tcggtgatct tttctgcatt ctttacagcg attcttggat ggatatacgc atgtgtgcct
    14701 tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat catgcaaaag tccagcacgc
    14761 gctgttgctt tagcattgcc accccatttt tttgcgagca gataactata gtaagaaaca
    14821 ctgatggaat gctccaaacg agtggaatga acatgctgtg tgtaattcgc taattttttc
    14881 actgcttctg tttctaaaag atcttctaca taagacatat attcttcgtc ttcacgccat
    14941 ttttctgtca taattatcga tctgccacct ttttgaattt cattttcttc tgaaattgat
    15001 ctaagtatac caccagtgtt cggattattc tagtcgcctt ggataaaata gccaaagctt
    15061 aatagttcct taatcataaa gaaaaatgat aaagaacaat ccctgccgca accccaacat
    15121 tcaacgattc cgcatttcct ttcatcggga tatatacgtt ctgatcagtc aaagacaaga
    15181 tttcctgact taccccctga ccttcgttcc ccatgattat tgcgaagtta tctgattttt
    15241 ctactttgtc taaggcgtgt gcttcttcat ttaattctgt gccatacact gtaaaccctt
    15301 tgtctttcat taaaggaatc acttctgtca aatctttttg caacacaggc aaatgataat
    15361 tgcttccctg catgctgcgc agcacctttg tactataaat atctgccgtt cctttgccta
    15421 ataaaacacc agccatatca aatgcatctg ccgtacggat catcgtccct acatttccag
    15481 gatcttgtac attatctaat aacagccatc ctcctgaaaa atctggcgtg ccttcagccg
    15541 ttaacgccat cactgctaag attccttgtg gtgtaggtag atccgataca gcatccatga
    15601 cctcatccga aactagaaaa gtagaaatct ctttgtttgc ggccagccag tctccccatt
    15661 ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc tgcatcagac gacgcagctt
    15721 cttcaatcag atggaaccct tccaaaagat attcttcctt ttcttcccga tatttctttt
    15781 tatgtagttt ttttaattct ttgattcttg gattttttgc tgattggatc tctttcacat
    15841 tcctcacctc cccatgatta tatcatggaa taggtgcttg agcttgtttc atcctaaaaa
    15901 aatcggtatc attaatttat aaaataggaa agaaaggtga cgaacatgcg caaagttaaa
    15961 atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct acatgaccaa gatggtcgct
    16021 gatcaactag gcgtgactgg aacggtaaaa aacgaagatg acggctcagt cacgatcgaa
    16081 gcaatcggca atgacgacat catccagaaa tttattgaag aagtcaagaa atctccgagc
    16141 ccaagtggac gagtgcagta tgtggatgtc caagaagacc cgatgattga ggaacgaaag
    16201 aaatttgatg tggtgggata aggttgtgaa aaagctggtc tgcatcaagc attaaggaca
    16261 ggttgtgaaa aatagctctc catatttttc aacaggctgg acttaatgcc gtagatgcaa
    16321 gcttttgaac accgtcaata aggttgtgag aaagtcgctt agttttgagt agtaaggaga
    16381 attttgtgaa aatggctttc cacatttttg cataaaattt gacttaatac cgagaaactg
    16441 acttttgaac accgtcgata aggttgtttt tgagacgctt tgcctcaagt attaaagata
    16501 ggtagtaaaa aagctgatct ataacttgca ataaatttgc ttaacccata ttttccagac
    16561 agaacaaaaa agtttttagt tttttaaata agaaagaaac acttggaatc actcttttct
    16621 ttccaagtat ttctttcttg attttttgtc tttttttcaa aatttttcgc tacgtaaaag
    16681 aacgaatatt tatatgaaat ttctatgaag ttgattgaat tccatgcgct tttttagtat
    16741 agttaagttt gtgtaaaaat tatagaagag gaagtattct atgaataaat ggaaaaactg
    16801 gttattggga tccggacttg tcagtatcct gctcttttta tctggctgtg tgcggatgga
    16861 ttcaaacgga aaccctgaca cttcgggaat cgtttatcga gtactcgtcc atcctatggg
    16921 ccaagcgatt acatatctag ttgagaattt caattggtct tatggctggg cagttattgc
    16981 aatgactgtg atcgtccgta tcatcatctt gccactaggt attagtcaat cgaaaaaaac
    17041 aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag cccaacaaaa
    17101 attgaaagct gcaacaacac gtgaagaaca aatggcagca caagcagaaa tgcagcaagt
    17161 ataccgcgaa aacggattga gtatgaccgg cggaatcggc tgtctgcctc tattgatcca
    17221 aatgccgatc ttctctgcct tgtactttac ggcacgttat acagaaggta ttcgggaatc
    17281 atctttctac ggcatcgatt taggctcacc aagtatggta cttgttgtca ttgcaggggt
    17341 cgcctatctg cttcaaggat acatctcaac gatcggtatt cctgaagaac agaaaaagac
    17401 gatgcgtacg atgctaatcg tctctcctgc tatgatcgtc ttcatgtcga tcagtgcgcc
    17461 agccggtgtt acactttact gggtagtcgg tggtgtgttc agctgtctgc aaacattcat
    17521 cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg gcagaagagt tgaaacaaaa
    17581 cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca gaatcagcaa agccagctaa
    17641 caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac agtacaaaca acaaaggccg
    17701 caacgcagga aagcagcaga agcggaaata aaaacatagg acaaaaaaga tgagagctaa
    17761 gccctcatct tttttgttac tcttcctctt ctgctattcc attaattaat tcgttgacag
    17821 agacaatgct ttcacatcct gcacgaacaa actcgggctc tgaaccaatc atttgtgagt
    17881 tgataaattc aatgaccatc ggaagattca tcccagcata aagagtaatc tgttgtcctt
    17941 ccattattag tttagaaatg atatttgcag gtgtaccacc taaaagatcg catagtacca
    18001 caaaatcatt taacgaatct gtgacagtga gaaacttttg tttgtatgtt tcagtgccat
    18061 cttctggtaa taaaggtaac gtgtagatat cctcttgagg ccccatgatc atttctgtac
    18121 tttttttcaa ttcctcacaa aattttccgt gactaatcaa taccaattgt ctactcattt
    18181 ttcgctctcc cttatttcga aatcacaccc aatgcagata aagcaacaca cacaattaat
    18241 acgataaaaa tcgctcgagt aggagtcatg tttttctttc ctaataacca ataaacacca
    18301 ccgacgatcc cagcaggtaa aattttaggt aagatcatat cgatatagtt ttgcatattc
    18361 aatgtcacat cgccaatcgt tggtgcccat gaaaatttaa cattgatcat tgtcgcaact
    18421 aatgccccta ccatgaatac ccctaataaa gtggctgcgt ccgttaatgc agttaagcga
    18481 gattgcatag tagtaactaa ggaaattcct tctttgtatg caaattcaag ttgcttccaa
    18541 cgaaaaacca tgacaacgat ctgagcaaaa atccatataa acacacctat cggatttcca
    18601 tcagttgcca tattcgccgc caaagcccca aaaatcgttg gaatcaaagc agcaaatacg
    18661 gaatctccaa tcgctgcaaa ggaacccatc aatccagctt tcagccccga tacggtttcc
    18721 tttgaagcaa ttccctcttt ttcttccatt gcaagatcaa tcccagtaat gattgtatta
    18781 aaaaaattag aagtattaaa aaattgcgta tgtgttttca tcatctcttt taattctggt
    18841 gtattgtctc catatatttt tcttagttgt ggcaagatag tatataaata gccagaaccc
    18901 tgcattctct catagttcca acctaattga aaagtgaaca aacttcttcg attgatttgc
    18961 ttaaaatctt cttttgttaa tttgtaatta gatttcgtca tcttcgattt ccccctcaac
    19021 agaatgtgat ggctcactag ctaatggttg atggtctttg ttttgatcat tcttataact
    19081 gatcattgct agtgctagac caatcaatgc tacagcaagc atcggcagcg aattgaaagt
    19141 tgccggaaga tctttaacaa cgcttgccac acttgttcct aataattgaa cattagagaa
    19201 taacgttgct aataatgccg tgatcgtaaa tcctaaaata agataagata gatgtttttt
    19261 tactggtaaa tagcgcagaa gaatggcaaa cccaacagct ggtaaaacag ctcctgctac
    19321 tgaaagtcca tcgcctagcc attttagatt cccgtttaaa acagaaacaa cattttcaac
    19381 taatccacca ccaaatgcta aagctaaaaa tacaggaatc atacgagaga gcgaccaagg
    19441 taaagctccc attaagaaat tgcgttcgat tgcttgataa ttcatgtctt ctacgtgacg
    19501 gtcaatacga tgggcaaagt atgtgttagc gaatctagct aaaatatcca attggatcat
    19561 taagcttgct acaggcactg caatagcagc aatcgcttgt tctggattca ttccaagaga
    19621 aatcgagaag gcagttgcta aaatcgtccc agagttggca tctatttttg atgcgcctcc
    19681 aaacgtcccc actcctaaaa ctgtcaactg catactgcca ccaataatca aacctgcgtt
    19741 taaatcaccc attactaaac ctgcatataa ccctgcaaac actggcgcac tcattgaaga
    19801 atagatctgt aattcatcta aaatttgata acctgcatat aatgttaata ataagatctg
    19861 ccaccattga atatccatgg atcattcctc ctatttgatt aaaagattca taaagtcttc
    19921 tgctttatcg tttggtacca tttgagcaat taaatgtata ccaagctgat ctaatttttt
    19981 aaatacttcg atatctgcat ctaccacatt gattgatttt gtaatggacc gtgtttcatt
    20041 cgtttgtgac atattcccca catttacttc tttgatagga actccttttt ctactaaaga
    20101 taagattcga tctggctttt ttgctacgat caatactcgt tgagagtcat atttgccagc
    20161 taaaatattt gttgctgctt tttcgattgg caaaatgctt aattttaccc ctgctggagt
    20221 cgctaacttc aatccacttt tttcaattgt gtttgtggct acctcatcat ccacgaccat
    20281 gattcgacta atattcagtt tcgttgtcca aagatttgct acttatccat gtatcaaacg
    20341 tccatcgatt cttacaccaa taattgccat catgcattct ccttatctat tttttctatt
    20401 tattgttcaa ttaaaactgt gacatcatca aatatccctt ctgtttcaaa gataacgttt
    20461 tcattttttc tttcttatat ttcattttta agctaagaac aaattccttc ccaactaaag
    20521 aaaacgcttt cttttatatt taagttcatt tgattttctt aacttgaatg tgtttcatca
    20581 acatcgtaac tttcataggg tcgatccagc cagttttctc ttgttgcgcg tttgccatac
    20641 cacaagccat agaaaatgcg agaatctctt cgattgattg cctttttgat aacgcatggg
    20701 ccgatcctgc aagggtcgcg tcacctgaac caacaggatt aacaactgat attttaggaa
    20761 taatgacttc gtaaaaacac tgagcaactt ttgccacagc acctctctct cctaaagaaa
    20821 caacaaccca tggcactcct tcaaatattg gatgagctaa taaggactga attttttcta
    20881 attgaatcga agaacagata cgttggccaa tcagttctcc taattcttcc agattgggct
    20941 tgataagtag tggcttctcc ttaccttcca atacttcaag caaaaattta ccagaagtat
    21001 ctagtaatat gtctttccct tttttatcag ctaaagcaac caattgctta tagtaatcaa
    21061 gagggcatcc ttggggtaaa ctacctgaga tcgtcataat atctgatatt tcgaggagct
    21121 gctcgaaatg atcggtaaat gtctgttgtt ctttctttgt tatgaagggg cctcgttcca
    21181 aaagctctgt ttgtctcgaa ctatgcagga tagcaataga atcccttgtc tctcctttaa
    21241 ttggaacaaa ctgatgcatg atccctaact gatctagctg gttctttaga tattcaccgt
    21301 gctggccacc tattaaacca gtagctatca cctcttctcc taataaattt agaaccctcg
    21361 ccacattcac tccttttcct cccgctgttt tattgactgt ttcaactcga ttgactttgt
    21421 cgaggtttaa ttcttcaatt gtatacgcga tatcgatgga aggattcaag gtaactgtcg
    21481 taatcatttc atcttcacct ttttgctttt tgttttattg aaccaagggg tagcggtttc
    21541 atttattact ttatttaaag tctcgatgtt ttctctccca tttgttctta accaatgaat
    21601 agctccgtct tttccatttt cgacgtatgc tttgacccca tctttccatg tcgcacgtcc
    21661 acacaataca ccattgaagg tggaacccga ttcatgagca aaaatcagtg tttgttgaaa
    21721 taattctgtt gaaacacccc cactcaaaaa aatgaatggt aatggagtcg cttcactttg
    21781 ctccttaaag aaggcagcag cctcttcttt gctgtgaata aattcacctg tggaatagcc
    21841 attcacataa ttcatattga ctggtacttc tactttcaac acatcaactt tatattttgg
    21901 atcagaaaat tctttcatca tctcaataac tttgtggggc ttgactttcg catactctgg
    21961 agaagaacta tcgcttattt gtgcgtcata agaaactaac tccaaataga agggacgatt
    22021 ttctccttcg cactcactac ctaaacgttc gatgaacaca tgcttcaaat gattgatttc
    22081 gggtgcttca tcaacatcgt agtaaagtaa gaattttact gcatccgctt gctgttcttt
    22141 caatctgaga gaagaccaat ccgctaataa atcaggtaga cgaccaggcg tagttgcgtc
    22201 atagccggtt ttctcataag ctaacaataa ccccgtctct tttgctctga cttttgctgc
    22261 tggcaatcca tattccggat ccaataaaat agcagaagca tagggtgtca gctcagctga
    22321 tactagttct ttgaattcac tgatttggtc ttcacttggc tcttttccca acgatttgat
    22381 catttttttt agagcaccac gctgatcgat cgctaatgca ctaataatcc catcttttgt
    22441 tgatagtctc tccattgccc tatttttttc ttcattcatc tgtcatttct ccttcggttt
    22501 catacttatg aataatgacg cccttgacta ctcgattgac cgtgcctgtt ggagatggtg
    22561 tatctggttt attagctact ttgattgaag tatagagtgc aatgatttgt gcagtcataa
    22621 tcatcgctaa tgcaagatag gcatcgggca atttactcac cgagtcttcc aatagcacag
    22681 tcatacctga gaaatcaata gtacccggtt gcgcaatcgc aatgacattg gcagtgaccc
    22741 gatcttcttt tatttcctct aaaatatcta agtcataatt tcgcgtatat ggttgattgc
    22801 tcacaaaacc tacaacgagt gtctcgctat ttaaaaaaga ctttggtcca tgacgaaacc
    22861 ccatcgaaga atcaaaaatt gtagcaactt tacctgctgt taattctaaa atttttaatt
    22921 gtgcttctct tgtcgcacct gccaaacttc ctgaacccag ataagcgatc cgttcaaaaa
    22981 aatcaggtaa taacttttga atttcttgat ctcttgaaag aagatcttga ccgattttaa
    23041 ctaattgttc aagatatctt tcttttttct ccattgctgt ttgatcaaag atccataaag
    23101 cactcaataa catacacgta aaacttcctg tcatagcaaa acctgcatcg tttgctctct
    23161 ctggcatcaa taacagataa ttctgtttat catcctgtgc gttttttgcc aacatcccct
    23221 ctttagcaca agtaatcgtc aaatgaaaca atgtttcaac ttgttcattt gcaatttgca
    23281 cagccgctac actttctgga ctgttgccac tgcgagcaaa tgaaactaat aaagtcggtt
    23341 catttggaaa caaaaattct tttggtgaag ctacaatatc tgtcgtgcca aaactgtgga
    23401 agataaaaaa ctgattgtct ccatgttcat ttagataggg taagagcgta tccccaatat
    23461 attgagacgt tcctgcaccc gtaaaaataa cacgtattct tcttccgttt gcttcttttt
    23521 ttatttgatc aaaaaataga gctaaatttt cttggctttg catcaatcta tcgattgttt
    23581 ctttccagag atctggttgt tgttttattt cttgtgtcgt gattgctgca cctatttgtt
    23641 ttaatttgtt cttcgatagc tcaaacatgt tgttctccct ctcaatcatt ttcggatatg
    23701 ccgaatttta taatgaaact ggtccgctct tgctacacta tctgtatatt cgataatctc
    23761 atttttcacg ttataggtta tcctcgtcag atgtaagacg ggagcgccat cagcgattcg
    23821 tagcacaaca gcatcttttt tttgcacgat acttgcataa aactcttcat cagctaattg
    23881 aacttgttgt tcataatcct gtgaaaacac atcatacaac ggtttacttt caagtaacgg
    23941 caaatcaagt cccataaata gtttcgccgg tagataagaa cgctcaacca tcaatggctc
    24001 ttcatctgcc aaacgcaatc ttttaagttt aaatacagtt tcacctaaat ggatgttcaa
    24061 attcttagca attctcttat tagcttccat ttgtctaaat tctaaaatga ccgtcttagg
    24121 gacgcgccct atttcgcgca tttcttctgt aaagctatat gctccagcta agttcgatga
    24181 cttatcagac aaatcagaga cataggtacc ttttccatgt cttcggtaaa tataaccttg
    24241 attttccaat tcctgtaaag ccagacgtac ggtggtacga ctcaccccat agtgttgaca
    24301 aagttctctt tcagataaca gcttatcatg cggagtcatc tgtgtttcga tacgatcctt
    24361 caataaatca actagttgat aatacaatga ttttccttta tttgagctct ccataaaaat
    24421 cacctcttct ttactggtta taaccacaac acaagaatag tgaattatta atcatttgtc
    24481 aaaggttttt tagaccaaaa aagctattat aacagctctt taaagcttta tatcttgaat
    24541 agacaaagat acctcaaaaa taccagttac caataaaaga acagaaaaaa agacgaacca
    24601 cataagcgat ccgtcaaaat ggtctaactt atcgagcagc gtgatccaca ttaatcccta
    24661 tgatattcct acaactatcg ttttattact tccaatatca tttacaacaa ctaaaaatag
    24721 cttattaatg acaaatgtta ttattagttt tagataactt ttgcagtttt atactttttt
    24781 tctataaaat ttgacaaaca tttttttaaa taactaatta ttttttgaat ctgtcactca
    24841 gacaatcctt tctcttactt gttaacataa atagaagttg ctgtttcttc taatagtgta
    24901 cttaattctt taatattttg ttttccttct tctaacatcc aactactcgc tttttcttcg
    24961 ccttcggtaa caaaaggtgc tactcccttc gcccatgttg ctcgcccaca taagacacca
    25021 ttgaatgtcg aacctgattg ttttgcaaaa cgcaatgtat cttgaaatag tgcagcgctg
    25081 actcccgcac ttaaaaaaat aaacggttgt tcagtcactt tcgattgttc atagaaatac
    25141 gcggatgcct ctgcttgact ataacaagta tcttgcccat atccttccac aaatctcata
    25201 ttcactggta cttctacttt caatacatcc acttgataac gttcttttga aaattctcgc
    25261 atggcttcaa tcactttatg cggttttact tttgcatagt caagagaaga cggatcgcca
    25321 atagttgcat cgtaagaaag gagctctaaa aagaaaggta tgccttctgc ttcgcattct
    25381 gaaccaatcc gttctataac tatttgtttt tgcacattaa tctctactgg ttcatcaacg
    25441 tcatagtaca ataaaaattt acaagcatcc gcacccactt cttttaacct tttcactgac
    25501 caactattca gtaaatctgg tagtcggcct ggcattttcg catcatagcc ggttttttca
    25561 taagctagca acaaaccaca ttctttctct cgaagcgctg ctgcaggcag accatattct
    25621 ggatcgagta aaatagcgga agcatacgga gtcaggtact gagaaaccat ctgtttaaat
    25681 tggatgatat cttctgatgt agcttcttta tattggttga tcattttctt caaagcacca
    25741 cgttggtcga tagctagcgc gctaataatt ccagcttcat ccgtcaatcg cttcaaacgt
    25801 tcctgttttt ctatactcat tttcttcata tatcttcacc tatttcttta gttaatattg
    25861 tagctatttc tatttttgat tttgcagctc tcaactgttc tgtaacctct tttcgcatca
    25921 atgatcgtgc aacgagagac aataatttca aatgagtcgt tcctgcttct gaatctggga
    25981 taaataacgc aaaaataaaa tcaactgact gtctatctaa actgttccat tcaattcctt
    26041 ttttcagtgt gacgataatc aaactaggtt cattgattgt ttgagacttc gcatgaggga
    26101 tagcaaaacc tgacattatt cctgtagtac cttctttttc tctttccaga agtttttgat
    26161 atacttcttt ttcgtcatga gcgattccta aacagaatgt ttgctttgct aaaaacttaa
    26221 atgcttcttc ttgattatct aatggctgtt gtaaaaaaat gttctcttca tgaatgattg
    26281 tcatgataaa cctcctgctc ttccttcttc gataactgta cgatttgttg ctttattatc
    26341 cctcttcttg tctttatcag ttactctttt cttttggatc aaacctagta tcactccacc
    26401 aacgatcgaa ccaataagaa tcgctgtaac ccattggatt tttcccgtca ctacaggtaa
    26461 cactaaaaaa ccgccatgtg gggctggtac ttggacacca aacagatagg tcaagacagc
    26521 agcaattgat gagcctaaca tcatcgttgg taatactcgg atcgggtctt tcgcagcaaa
    26581 cggaatagct ccttccgtaa tatgagttga tcctaaaata aaattaacca aacctgcatt
    26641 acgatcatta gtatcaaaat atttaccaaa caataaagta gcaaaaccag taatcaacgg
    26701 tggtgcaatg caagctgctg acgcaccagc cataaaagtc gtgtttcctt gtgtcaaaag
    26761 agctgttcct gttacatatg ccgccttatt aaccggtcct cccatatcaa acgcacacat
    26821 acagccaaca atcacgccaa gaacaagtgg attcgaatat tcaaatccag ctaaaaagct
    26881 catcattcct tcatttatag ccttcattgg acctgccagc aaagacatca cgatgccaat
    26941 aaatgctaca ccaataactg gatataagac gattgccttc aagccatcta gttgtttagg
    27001 taaaattatc agtagctttt gcaaaaataa gataaaataa cctgctaaga acccagctaa
    27061 aatacctcct aaaaatccag taccgccatt catggctacg attccaccaa caaatccaac
    27121 gactaaccct ggacgtttcg cgatcccttc tgcaatataa gctgctaaaa ctggaaccat
    27181 cattcccatt gctgcatcgt caactgcttt taccattgcc gctgtagcat tgtattgtgc
    27241 atgatctgga tcagaagaat agattcccca aacggcaaat gaaatagcaa tcaatactcc
    27301 gccagctacc acgaaaggca acatatgaga gacaccgttc atcaaatttt tatagatgct
    27361 acgaccaaaa ttagcaccac ttatcgcttc tggttctaaa gtatcagcag cttcattatc
    27421 tgctaagaca gtaccttttc ctgccaatgc ttcgtcaatc agccgttgtg catccttgat
    27481 tcctgtactg acagaaacat caatgactcg ttttcccaca aatctttctg aatggacatc
    27541 tttgtctgcg gcaataatga cagcatctgc ctgtttgatt tcttcagccg ttaattcatt
    27601 ctcgatccca atttgtccat gggtctccac tttgatcgtg atgccttttt tcctagctgc
    27661 ttgctctaat gcctcttgcg ccatgtaggt gtgcgcaatt cctgtcggac aaccggttgc
    27721 tgcaattagt tgatattttc ccatctttcg aacctctctt ccttgatttt gatttgttta
    27781 cttaattcct ccacatccaa aaaatcagtc agtcctttac gaaaagcagt agagctgcct
    27841 gctgctatgc ttttacgtaa cgtttcatca agcgatcgtc gattcatgta tcctgctaaa
    27901 aaagtgccta acatggcatc acctgcacat gctgtattga caacttttcc ttttggcgag
    27961 tttcctcgaa tcaccctgtc tttcgtaaaa agaatcgctc ctttctcacc gagggataac
    28021 aaaacatttt ctgcgcctga ctctactagt cgttgtccat aaacaaagta atcttcatct
    28081 gtaaccattt ttcgtccgaa ccaagaagaa agttcttcct catttggttt cagcaaaaac
    28141 ggtcgatagg gtaaacaatc catgacttct tgatcactac tatcaataat caaacaaatc
    28201 cgactgattt ctattaaaac ctttggagac agccctcttg gtaaactccc agaaacacaa
    28261 agataatccc ctgcttgtaa tgctcttatt tgatttaaaa aattatgaac agctgtctgc
    28321 ggtatttcag gtccttgatt cactaatttg tattcttctc ccgtttgatt gacttgtgta
    28381 aatacattga tacgcgtcat accaggcact tcaataaact ctgtttggat ttgtttctgc
    28441 tgtaaatagt cttctatata ctttcctgtg aacccagcag aaaaacctaa tgccgtactt
    28501 gggaaatcta gcatctttaa tactagtgac acattcaccc cttttccgtt tgcctgaatg
    28561 tcatcctctt ttgtgcggtt taccataaaa ggatgcatct catctgtttc aataaataga
    28621 tcaattgcca aattcatcgt gcatgtgtag atagacataa ccaacgacct ccttgattct
    28681 attatgaaat acgcgaaata gaaaatgttt tcttaatcgt cccaacacaa agaaaacctt
    28741 tacatttata aactcgtcgt gcttttttta actaactttt ggtgaaagaa ggaaagaata
    28801 tgaatcacaa attaagtgaa acagagcagt acctctggaa ctttatcgaa cacctcatcc
    28861 tagagatccc taactattcc atcgtgaaac taagtgaaca agcaaatgtc tcaacagcca
    28921 ccattgtgcg gacaatgaaa aagaaaggct atgaagggtt tacttcgttt aagcatcatc
    28981 taaaagaagg agaaaacaag aacatcaatt tttcattttt agataaagta gacaaaggaa
    29041 tccgccgagc cattctaaaa aatgaacaag aagtggtacg tacaatcaat atgttggaaa
    29101 tcgggaacat tgaagatgca atacaaagaa tcaaatttgc tgaccgggtt tttgtctttg
    29161 cccgaggatt ttcagaaatg attggccaag aaatgctggt aaaacttcaa ttaacgggaa
    29221 aaaattgtca gatgcatact gatccagaaa ttatcaaaag catcagtcgg aaactaacta
    29281 agaaagacag cgttattttt gtttctttga acggtgagaa aacaaataaa aacatcaaaa
    29341 ataagattta tgtctttatt tttcaatttt ttaaat
//
LOCUS       DACJGB010000031        29098 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.031, whole genome shotgun sequence.
ACCESSION   DACJGB010000031 DACJGB010000000
VERSION     DACJGB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.031"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..1030)
                     /locus_tag="IYI34_001423"
     CDS             complement(<1..1030)
                     /locus_tag="IYI34_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0559296.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="IYI34_001424"
     CDS             complement(1112..1801)
                     /locus_tag="IYI34_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAR0559297.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IYI34_001425"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="IYI34_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAR0559298.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="IYI34_001426"
     CDS             complement(2328..2831)
                     /locus_tag="IYI34_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAR0559299.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IYI34_001427"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="IYI34_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAR0559300.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IYI34_001428"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="IYI34_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAR0559301.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="IYI34_001429"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="IYI34_001429"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAR0559302.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="IYI34_001430"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="IYI34_001430"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAR0559303.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="IYI34_001431"
     CDS             complement(6443..7462)
                     /locus_tag="IYI34_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAR0559304.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8522)
                     /locus_tag="IYI34_001432"
     CDS             complement(7635..8522)
                     /locus_tag="IYI34_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR0559305.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IYI34_001433"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="IYI34_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAR0559306.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IYI34_001434"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="IYI34_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAR0559307.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IYI34_001435"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="IYI34_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAR0559308.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="IYI34_001436"
     CDS             9753..10397
                     /locus_tag="IYI34_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR0559309.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="IYI34_001437"
     CDS             complement(10453..10956)
                     /locus_tag="IYI34_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAR0559310.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="IYI34_001438"
     CDS             11553..12518
                     /locus_tag="IYI34_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAR0559311.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="IYI34_001439"
     CDS             complement(12617..13789)
                     /locus_tag="IYI34_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559312.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="IYI34_001440"
     CDS             complement(13776..14921)
                     /locus_tag="IYI34_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559313.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="IYI34_001441"
     CDS             complement(14935..15867)
                     /locus_tag="IYI34_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559314.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="IYI34_001442"
     CDS             15984..17063
                     /locus_tag="IYI34_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAR0559315.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17678
                     /locus_tag="IYI34_001443"
                     /pseudo
     CDS             17060..17678
                     /locus_tag="IYI34_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17788..18015)
                     /locus_tag="IYI34_001444"
     CDS             complement(17788..18015)
                     /locus_tag="IYI34_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR0559316.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18118..20706)
                     /locus_tag="IYI34_001445"
     CDS             complement(18118..20706)
                     /locus_tag="IYI34_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAR0559317.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20726..21385)
                     /locus_tag="IYI34_001446"
     CDS             complement(20726..21385)
                     /locus_tag="IYI34_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR0559318.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21434..21937)
                     /gene="trmL"
                     /locus_tag="IYI34_001447"
     CDS             complement(21434..21937)
                     /gene="trmL"
                     /locus_tag="IYI34_001447"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAR0559319.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22158..23024)
                     /locus_tag="IYI34_001448"
     CDS             complement(22158..23024)
                     /locus_tag="IYI34_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAR0559320.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23133..23774
                     /locus_tag="IYI34_001449"
     CDS             23133..23774
                     /locus_tag="IYI34_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAR0559321.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23836..25197)
                     /gene="mgtE"
                     /locus_tag="IYI34_001450"
     CDS             complement(23836..25197)
                     /gene="mgtE"
                     /locus_tag="IYI34_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAR0559322.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25215..26114)
                     /locus_tag="IYI34_001451"
     CDS             complement(25215..26114)
                     /locus_tag="IYI34_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAR0559323.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26116..26913)
                     /locus_tag="IYI34_001452"
     CDS             complement(26116..26913)
                     /locus_tag="IYI34_001452"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAR0559324.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26891..27595)
                     /locus_tag="IYI34_001453"
     CDS             complement(26891..27595)
                     /locus_tag="IYI34_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAR0559325.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27726..28301
                     /locus_tag="IYI34_001454"
     CDS             27726..28301
                     /locus_tag="IYI34_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAR0559326.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28419..29096
                     /locus_tag="IYI34_001455"
     CDS             28419..29096
                     /locus_tag="IYI34_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAR0559327.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8561 a         6167 c         5232 g         9138 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc gtaacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgcgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc
    17221 ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc
    17281 ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg
    17341 tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt
    17401 ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga
    17461 ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca
    17521 tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg
    17581 acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca
    17641 cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac
    17701 ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg
    17761 caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg
    17821 attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga
    17881 ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat
    17941 ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc
    18001 ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga
    18061 aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca
    18121 agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc
    18181 tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc
    18241 tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc
    18301 cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt
    18361 tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga
    18421 atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat
    18481 ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc
    18541 ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa
    18601 attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc
    18661 actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt
    18721 tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc
    18781 agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa
    18841 ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca
    18901 atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa
    18961 cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg
    19021 gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc
    19081 atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc
    19141 ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg
    19201 attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg
    19261 accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat
    19321 cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa
    19381 aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc
    19441 cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc
    19501 tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc
    19561 caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt
    19621 ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc
    19681 ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt
    19741 ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga
    19801 attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg
    19861 ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat
    19921 ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg
    19981 tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg
    20041 aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt
    20101 gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa
    20161 agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg
    20221 attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat
    20281 ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat
    20341 tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat
    20401 tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc
    20461 ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc
    20521 ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc
    20581 tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt
    20641 actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg
    20701 ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta
    20761 aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag
    20821 tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct
    20881 cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa
    20941 gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg
    21001 ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca
    21061 aattcggcag attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag
    21121 tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct
    21181 ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt
    21241 taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca
    21301 aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc
    21361 catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc
    21421 tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag
    21481 ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca
    21541 atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca
    21601 taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat
    21661 cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac
    21721 gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat
    21781 tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct
    21841 cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg
    21901 cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta
    21961 tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt
    22021 gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag
    22081 gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt
    22141 tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat
    22201 aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc
    22261 tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt
    22321 atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg
    22381 gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga
    22441 cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag
    22501 agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat
    22561 gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat
    22621 tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc
    22681 tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc
    22741 gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat
    22801 tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg
    22861 gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc
    22921 aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga
    22981 ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc
    23041 tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct
    23101 agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact
    23161 acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact
    23221 tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg
    23281 gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata
    23341 acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg
    23401 atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag gccttagaac
    23461 tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc
    23521 ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct
    23581 tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat
    23641 tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga
    23701 atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg
    23761 atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca
    23821 aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat
    23881 cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc
    23941 aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat
    24001 tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc
    24061 tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc
    24121 agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac
    24181 agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa
    24241 gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt
    24301 cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac
    24361 tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc
    24421 ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc
    24481 ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc
    24541 ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc
    24601 gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg
    24661 attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta
    24721 catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc
    24781 gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga
    24841 catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc
    24901 attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag
    24961 gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt
    25021 aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg
    25081 cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc
    25141 ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc
    25201 aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa
    25261 ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa
    25321 tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc
    25381 agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc
    25441 tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc
    25501 gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc
    25561 aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat
    25621 tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc
    25681 tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac atggattact
    25741 tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt
    25801 ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt
    25861 tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct
    25921 tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct
    25981 acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt
    26041 gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc
    26101 catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg
    26161 catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg
    26221 gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc
    26281 caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa
    26341 cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat
    26401 cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa
    26461 tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt
    26521 acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc
    26581 atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt
    26641 tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa
    26701 atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa
    26761 cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc
    26821 tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt
    26881 tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt
    26941 tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc
    27001 gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag
    27061 cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat
    27121 aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt
    27181 ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat
    27241 aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca
    27301 tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca
    27361 tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc
    27421 gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa
    27481 tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca
    27541 attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg
    27601 atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta
    27661 ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg
    27721 atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg
    27781 agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt
    27841 atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg
    27901 ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa
    27961 cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt
    28021 caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa
    28081 ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt ttagctattg
    28141 atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat
    28201 ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg
    28261 ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa
    28321 ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag
    28381 tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg
    28441 ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag
    28501 aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt
    28561 tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac
    28621 gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc
    28681 aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg
    28741 acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga
    28801 atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa
    28861 aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct
    28921 gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca
    28981 cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata
    29041 caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaa
//
LOCUS       DACJGB010000032        28621 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.032, whole genome shotgun sequence.
ACCESSION   DACJGB010000032 DACJGB010000000
VERSION     DACJGB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.032"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(119..700)
                     /locus_tag="IYI34_001456"
     CDS             complement(119..700)
                     /locus_tag="IYI34_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0559328.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(883..1509)
                     /locus_tag="IYI34_001457"
     CDS             complement(883..1509)
                     /locus_tag="IYI34_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR0559329.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1531..2862)
                     /locus_tag="IYI34_001458"
     CDS             complement(1531..2862)
                     /locus_tag="IYI34_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAR0559330.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3017..3418)
                     /locus_tag="IYI34_001459"
     CDS             complement(3017..3418)
                     /locus_tag="IYI34_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAR0559331.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3504..3710)
                     /locus_tag="IYI34_001460"
     CDS             complement(3504..3710)
                     /locus_tag="IYI34_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559332.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3805..5307)
                     /locus_tag="IYI34_001461"
     CDS             complement(3805..5307)
                     /locus_tag="IYI34_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAR0559333.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5325..5780)
                     /gene="argR"
                     /locus_tag="IYI34_001462"
     CDS             complement(5325..5780)
                     /gene="argR"
                     /locus_tag="IYI34_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAR0559334.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5878..6495)
                     /locus_tag="IYI34_001463"
     CDS             complement(5878..6495)
                     /locus_tag="IYI34_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559335.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6716..8089
                     /locus_tag="IYI34_001464"
     CDS             6716..8089
                     /locus_tag="IYI34_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR0559336.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8164..9276
                     /locus_tag="IYI34_001465"
     CDS             8164..9276
                     /locus_tag="IYI34_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAR0559337.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9348..10985)
                     /locus_tag="IYI34_001466"
     CDS             complement(9348..10985)
                     /locus_tag="IYI34_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAR0559338.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11153..11761)
                     /locus_tag="IYI34_001467"
     CDS             complement(11153..11761)
                     /locus_tag="IYI34_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR0559339.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12145..13296)
                     /gene="queG"
                     /locus_tag="IYI34_001468"
     CDS             complement(12145..13296)
                     /gene="queG"
                     /locus_tag="IYI34_001468"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAR0559340.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13315..14067)
                     /locus_tag="IYI34_001469"
     CDS             complement(13315..14067)
                     /locus_tag="IYI34_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559341.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14208..15158)
                     /locus_tag="IYI34_001470"
     CDS             complement(14208..15158)
                     /locus_tag="IYI34_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAR0559342.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15159..15998)
                     /locus_tag="IYI34_001471"
     CDS             complement(15159..15998)
                     /locus_tag="IYI34_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAR0559343.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15995..16741)
                     /locus_tag="IYI34_001472"
     CDS             complement(15995..16741)
                     /locus_tag="IYI34_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAR0559344.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16979..18349
                     /locus_tag="IYI34_001473"
     CDS             16979..18349
                     /locus_tag="IYI34_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0559345.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18404..19300)
                     /locus_tag="IYI34_001474"
     CDS             complement(18404..19300)
                     /locus_tag="IYI34_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR0559346.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19581..20258)
                     /locus_tag="IYI34_001475"
     CDS             complement(19581..20258)
                     /locus_tag="IYI34_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAR0559347.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20349..21254)
                     /locus_tag="IYI34_001476"
     CDS             complement(20349..21254)
                     /locus_tag="IYI34_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR0559348.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21429..22853
                     /locus_tag="IYI34_001477"
     CDS             21429..22853
                     /locus_tag="IYI34_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0559349.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            22899..23930
                     /locus_tag="IYI34_001478"
     CDS             22899..23930
                     /locus_tag="IYI34_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAR0559350.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23937..25124
                     /locus_tag="IYI34_001479"
     CDS             23937..25124
                     /locus_tag="IYI34_001479"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAR0559351.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25280..26662
                     /locus_tag="IYI34_001480"
     CDS             25280..26662
                     /locus_tag="IYI34_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAR0559352.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26789..27019
                     /locus_tag="IYI34_001481"
     CDS             26789..27019
                     /locus_tag="IYI34_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559353.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27219..27887)
                     /locus_tag="IYI34_001482"
     CDS             complement(27219..27887)
                     /locus_tag="IYI34_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAR0559354.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(28076..>28621)
                     /locus_tag="IYI34_001483"
     CDS             complement(28076..>28621)
                     /locus_tag="IYI34_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAR0559355.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         7975 a         5848 c         5250 g         9548 t
ORIGIN      
        1 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat
       61 tttcgtaaat tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt
      121 aaagaacctt ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta
      181 gtgatagatc ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg
      241 ctttggcttg acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat
      301 tttgtttcgt atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt
      361 catccactgt actcaactca ataatcaaat tacctacagg acagccatga taagtgatct
      421 gtgtctcatg atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt
      481 ttccagcatt taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga
      541 tcaccgctag acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg
      601 tatcagaagc tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa
      661 gatctttggt cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt
      721 tctcatagtc ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa
      781 gactgtgcca aattatctga cacagccctc actataaaac tgttctcata tagctagatg
      841 ttcattgatt caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa
      901 tacttcctct gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc
      961 cattgcttct tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact
     1021 ctctaccaat tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat
     1081 tactgtgtcg attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa
     1141 gattttatga ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat
     1201 aaaatcggct cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg
     1261 ctgctcaagc atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta
     1321 aaaataatga ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc
     1381 aatcatattt tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc
     1441 gcctgctggt ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt
     1501 attgaacatg gtggattctc cattcgttat ttattttaat acatcattta gagcttcact
     1561 cattgtcgga tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat
     1621 agctagcgaa atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag
     1681 aatcaagtca gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac
     1741 ttgtgcttta gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc
     1801 tttggcttgt ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta
     1861 aggcacagct ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat
     1921 gattcgataa tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat
     1981 cgcccaaatg ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc
     2041 tgttagttta acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt
     2101 tgcagccaaa acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac
     2161 ggcagctgtt gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat
     2221 tcccgcattt gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag
     2281 aaattcagaa tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc
     2341 aaattccaag ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg
     2401 atccatcgct tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa
     2461 aatgattggt tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc
     2521 accattattc gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac
     2581 tgtcccagtt gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa
     2641 tttttctttc cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa
     2701 tgatttagaa ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt
     2761 ttcaacgact aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc
     2821 ctttccaaaa ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt
     2881 ttacctaccg attggtacaa aaatatgata accgataatt gtctattctc caaatcatct
     2941 gctttatttg tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc
     3001 gatttccttt attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt
     3061 tggagtaagg gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga
     3121 tgccatcgat atgcacttct aatactacac cgatctgata gccgccatca tctaaattat
     3181 aaagagagac acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat
     3241 gttgtaccgc cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg
     3301 ttccttctgc atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct
     3361 cgacttctcc attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg
     3421 tactgctcct ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa
     3481 ggcgctttct acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt
     3541 ccaaaaaaca aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata
     3601 gcaatctttt ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag
     3661 ttgaccccat aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc
     3721 ttttgctggt ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc
     3781 aactagcctt tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg
     3841 tccgaataat tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc
     3901 tatctgtaat actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc
     3961 gtatggctct tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc
     4021 tttttcttct tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg
     4081 ttcatcgctg aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt
     4141 gatttgttca tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt
     4201 tgcgatgaca tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct
     4261 tgtttcgatg gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat
     4321 tacatttcga tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct
     4381 ttgtagatga gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa
     4441 tacgattgta cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact
     4501 gacaactgca gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc
     4561 taaaacagcc tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt
     4621 aggaaatcgg ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt
     4681 caatactttt tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca
     4741 accatttagt ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc
     4801 ttgcgcataa ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat
     4861 tttttgctgc tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt
     4921 aaagaatttt attccattat aataataggg aagatggcta gcagtcagca taaccgcagc
     4981 atcgcacgag aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc
     5041 gaaatcgatg acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc
     5101 tggtccgctt atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt
     5161 tgatttcttc tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc
     5221 tgtcgtcaag tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc
     5281 attttgcagc gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt
     5341 tccagctttt ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta
     5401 tctacaccgg ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt
     5461 accacatcgg caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata
     5521 cgcaaaacct cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc
     5581 tggttaaata atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt
     5641 tctcgaatgt ctcgagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc
     5701 gcaagcagct cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct
     5761 tgtcgctgtt tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt
     5821 aatatttctt catattttgg tattttttac ttttattttt tgttttcatt caggttgcta
     5881 acctttttct attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg
     5941 atcgctgatg taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat
     6001 gaatgcttct aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat
     6061 ttgggcacaa agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg
     6121 aagtttgcta gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg
     6181 tcggatcatc aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt
     6241 ttcttgcagt ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat
     6301 cagccaattt tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac
     6361 tcttcgttta aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt
     6421 tttaggaacc tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca
     6481 ttcaattttt tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc
     6541 tctttttgag tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag
     6601 tgaagtaaat aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta
     6661 tttttataat agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac
     6721 aaaacaaaaa cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc
     6781 gggcgtattg atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga
     6841 tttaacgact gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat
     6901 tgttccgctg tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc
     6961 aaatctgctt ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct
     7021 attgtttggt cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca
     7081 tattatcttg acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt
     7141 gacaacggcc attgctccag caattggccc cacttatgga ggcattatga cttcacattt
     7201 tacatggaat catatcttcc tgtttcttgt gcccgttttg tttctttctt tgattatcgg
     7261 ctggtttgct attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt
     7321 tttaggaatt tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt
     7381 ttccagctgg attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag
     7441 taaaaaagtg gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt
     7501 gtttttgatt ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc
     7561 gaatttcctt cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc
     7621 cggcgcagcg gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg
     7681 tccgaagaag ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt
     7741 cctactcata ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat
     7801 cggtatcgga ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga
     7861 actacaagga gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc
     7921 tacttcatta gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga
     7981 agtgtctact acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt
     8041 aagctttgct cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac
     8101 ggcaggtgtt gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt
     8161 tttatggaaa aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac
     8221 ttagatacat caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa
     8281 gctagcgctg ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt
     8341 ctggctaaag acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa
     8401 acgatttacg cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg
     8461 gctgttcttg ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt
     8521 tcacctgaac agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc
     8581 agtggtggtg gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca
     8641 caaaaacaaa tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg
     8701 ctcatcaagg gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg
     8761 gcacttaagc ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg
     8821 cagctcttag catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt
     8881 gacggagaag ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat
     8941 gaaaacgatc aaggctggga tttggctcct actagcgaag cttataaggg attgactaag
     9001 agtacagaac agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt
     9061 gtaggtaccc gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat
     9121 ccaagcttac tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc
     9181 ggctatctat tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag
     9241 gacatctcca cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga
     9301 tcccgagcga tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc
     9361 tgaatcaaat tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga
     9421 gcttcaatga atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta
     9481 tcctcaaaag aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca
     9541 aacgacttga attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca
     9601 ggtttgattt ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt
     9661 ttcaaaaaca actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga
     9721 ttagcaaatg gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg
     9781 tctgatcgtt ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc
     9841 ttgataattg gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg
     9901 ctaagatgat cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg
     9961 tttcgtatat ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca
    10021 ttctgacaag taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta
    10081 tcaaagtaag aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca
    10141 tcgtcatgca agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat
    10201 tttccagaac cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat
    10261 tgtttcatcc caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc
    10321 gtcaaagtcg ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta
    10381 tggagagcat caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca
    10441 caaaagatat aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc
    10501 ttcgaattcg ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct
    10561 ggaatcagca aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta
    10621 tggtaaaact ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg
    10681 ctttgttcct tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg
    10741 cttccatcat tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt
    10801 gagattgtta aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc
    10861 ttcctatcaa aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga
    10921 atactggata acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta
    10981 ctcatggctc ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga
    11041 gtttttattt gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac
    11101 cgagaacttg aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc
    11161 agccgtttgc taaccagaat gttttagcag catcccatga accataacga gatgttacat
    11221 attgttctgc tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat
    11281 ctaattgata acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt
    11341 tttgtgcgat ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag
    11401 caggagctac ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt
    11461 cagttgtaga tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg
    11521 aagttgggat tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt
    11581 tttcagcaat tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata
    11641 atgaatcacc tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa
    11701 acattgcagt agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca
    11761 tattaaactc ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta
    11821 gaaatatttc ataggtaaaa gaaacatgac atttaatgac aaaataacga attatttaat
    11881 atttgtaaca tttaaacaaa tatttttttg atacaaaacc ataaaagcct tgttgtataa
    11941 gcaaagtaat cgtttttcta tgttttttta tttttgaatg aaaaacgcag attctgtaac
    12001 attcactgca ataatccata aaaaaaagca aaaaaatttt tgatttttat caaattctct
    12061 taaaaaaatg taaaaaatac aaaaaaacca gtaattgtta caacaattac tggacaaaaa
    12121 ctttgaataa tgttaactat ttttttattt taactttctc cgcaataatt tctgggcatg
    12181 ttccaattcg tttataacga cttcttctgt ttctttagct aatatttcat ctaatacatc
    12241 aagccatttt tccggttctt ttgttcctag acgtccaata gcccaagctg ctgttcctct
    12301 gatgacaggg cggacatctg ttaaacattc tgaaatctca ggcaatgccg ttcgatcacc
    12361 tagattagct aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac gccatgaacc
    12421 agctaaatgc ccaaattgct gtttgaagtc tttattagac atcgaaagca ttggctttaa
    12481 ttttggatat atttcgtcta cttttggttc catctcttca tgaaaatgaa aatcttttcc
    12541 ttgattgtaa gggcagacta actggcagat atcacatcca taaatgacat ttcgcatttt
    12601 tttacgatat tcttccggca tcatcccttt tgtctgagtt tgataagaca aacatcgctg
    12661 ggcattcatt cttccgtcac caagcaatgc ttttgtcgga caagcagtca cgcagcgcat
    12721 acagtcaccg caaccaaatt ctcctggttt atcaggctca aatacaatat tagtaacgat
    12781 ctcaccaaga tagacaaatg aaccgaactc ttcagtgatc aacaatccgt ttcgtccaat
    12841 aaatcctaaa ccagctcgct gtgctacagc gacatcgatc agttctccag tatctacttg
    12901 cggagctaga cgccattcct cttctttttg ccatttttct gctcttgttt ggatgaattc
    12961 gatcaatcgg ttcaaacggt ctctgagtat atcatggtaa tcgatccccc aagaagcacg
    13021 agcaaactgc ccgcgttttt catccctagg cacttcttca tggatcctag taggatacgc
    13081 aagtgcaatg gaaatgatcg atttcggctg atcaaatgtc atttctggat aaagacgctc
    13141 atcgatattt ggatgttcaa agccagaagt atggccagct gctttttgtt cttccaacga
    13201 ttcttttaat gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa ttcctaatcg
    13261 tttgctttct tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt gctgttaacg
    13321 gctgtataac ccataatata gtttttcttt tttcagctgg acaaactgga tctgctgatc
    13381 ggcaatcttc ttttcttcca tccatttttt tcttgcagca aatacttccc aataatttgt
    13441 tgaaattaat tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa ctggttttgt
    13501 acttaacagt tcatggagcc ttacctcttc ccatggcgaa aactcagaca gatcgcggga
    13561 atacaatact tcttcataga gatttttttg ctgtgtgtac gtgaatgttt gcgggatttt
    13621 cccaggccta gcaatttcta ctagacgatt ataaaacata ttcattaatt tgctgttggc
    13681 aataaattga cttttataat aggccgactg gatctttttg attactcctt ctgctttgat
    13741 gactgaaaat aatgcaggcc atgctttgac ggttatttcg tcgttttgtt taactaatac
    13801 gccaggtgtg aagccgactg gagaaaggaa cacaaaataa ccgataccgg tattttcatc
    13861 taaaggctga aattcatcaa acaaccattt gcctatgtac agctcttcat ctggttctgt
    13921 tgtctcattt tgatttgcta tgaataattt agctaattct gcttcaagaa attgaaggtc
    13981 ttgatccttg tcggtatttt cctgctggct gatcaatcga aagacttctt ctttaatcgt
    14041 atatttatct ttacgtgtaa aattcatagc cacaccttcc ttccttactc taactataac
    14101 ggaatttctt caaaaaaggt atctgtacga cgaaaaacag cataattttt tttcatatct
    14161 cttataaaaa atgcaaggtc tgctcctcaa gctagacctt gcacttttca gctttctttt
    14221 gtttgtgaca aaccttcatg gatcgtttca atattccatt tcatcatctg atagtaagaa
    14281 tctccttgtt ctccttcttt tgctatggaa tcagtgaaca gtttatcata gattttcaaa
    14341 cccgtttctt ttgctacccg ttccatgctt cttgagtcta cactggtttc cacgaataaa
    14401 accggtactt cttttgctct tatctgatca atgatcgctt tcatttgatc aggcgtacct
    14461 tgactttctg tattgatctc ccatatataa gctgctggca aatcatatgc tcgtgaaaaa
    14521 tacttgaatg ccccttcact tgttactaat aattttttgt tctcttcgat agaagcaaaa
    14581 ctttccttcg cttgtgtatc taattctttt agtttttcca catatgcttc tgcatttttt
    14641 ttatagagtg tcgcattttc tgcatctttt tcagagaata tacgtgcgat ttcctccaca
    14701 tattttattc cgttagatag gtctagccaa gcatggggat ctgcttttgt atgttcttca
    14761 ccgctagtta aataaagagg ttctacattt ttgctaactg caaaataatc ttttccttct
    14821 tcttttttag ccgtttccat caagttatcg aaccagctgt tacctgtttc aagattcaaa
    14881 ccgttgtata aaataacatc tgcatcgctt gcctttttga tgtcttctgg tagtacttca
    14941 tattcatgcg gatctgttcc gactggtacg atactgtgga tatctatttg atctgttccg
    15001 acctcttttg ccatgtccgc caagatcgaa ttggtagcca cgactgacaa cttttcttta
    15061 cttccttcat ccgcttggtc agaagcctgc tttccgcaac cggctaatac caagaaacta
    15121 actgccaaga ttaaaaagag tgattttttt gttttcatct atctgcttcc tttctgaaat
    15181 aaaattcctt ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt acatgctaac
    15241 acgatcgtag ctcctgatgg ccaattgtaa ctataactga aaaacacacc gacaactgca
    15301 cttagcatac cgattcctgc agctgtaatg atcattttca gtaaatggtt cgttagcaag
    15361 taagccgtgg cggctggtgt gatcaacatg gcaatcacca agattgttcc tactgtctgt
    15421 aaactgacta cagccactaa tgtcaaaaag aacatcaaaa gataatggat cagcgatgtg
    15481 ttcagcccat aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa ttctttataa
    15541 aagaaaaaga caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa caaatcgctt
    15601 tccctaacag ctaagacatt cccaaataaa atatgataca aatctgtcgc actctgggca
    15661 tatgaaatca agataatccc taatgcaaaa aaggagctaa agacaattcc aatcgctgta
    15721 tcatttttca atcggctccg ctgagtcacg aaaccaatca ctgctgctgt tagcatccca
    15781 aaagcagtgg ctccaaagat gtaactaaaa ccaaacatat aagaaatggc tacacctgga
    15841 agaactgcgt gagagatagc gtctcccatc aaagacatcc ctcgtaaaat aataaaagaa
    15901 ccgatgactc cagaagcaag tcctacaagc atggaagtga ccaaagcgta ttgcaggaat
    15961 tgataattca ttaatccatc aataaaggat tggatcattt tttctctcct ccttttacta
    16021 aatgtctgat gatctcacca taggccaatt gaaggttctt cgatgtaaag acttcttgta
    16081 ctggaccgga agcgatcagc tgtttattta aaagaataac ctcatcgaaa tattcttcta
    16141 ctttatgaag atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc aattcctgca
    16201 agatgtcaaa gatctttcgt tcacttaacg catcaatccc tacgaatggt tcatctaaaa
    16261 agatccattc tgctccttgg gctagagctc ttgcaataaa aactctctga agctgtccac
    16321 ccgatagttc gctgatctgt cttttggcat attcttccat ccctactttt ttcaaagctt
    16381 gtcttgcacg ttctttttct tttttcccag gtctttgtcc aattcgtaaa gatggatatg
    16441 ttcctaaaag tacaacgcct aatacatcaa ttggaaagga aagatccaat tcacttcgct
    16501 gttccacgta ggctattttt ttacggactg agtttaccga ctgtccagaa aaggtgactt
    16561 taccagtagt agctggtatc aactctagca aagatttcat aaatgttgat tttcctgcac
    16621 cgtttggtcc gataataccg gtaatttttt gtggatttat cgtcaagttg atagttttca
    16681 aaacagtttt cccttgataa gcggctgaaa ggtcttgaat tacaatggcc gtacgttcca
    16741 aatcttacac tcctttttag gtttacctaa ctttttattt aaatatagtt taactaaaat
    16801 ggaaagcatt ttcaagtaga atgatgaaaa agtttttttc gaattttcct cgcattttat
    16861 gaaaattttc tgtagaatca catcaagagt tcaataaggc taacctaata atgaaaaaca
    16921 tgtgttgaaa cgtttgatta ttttttcttg ttgactcaac aacgaaagga gtttttgtat
    16981 ggaagaagaa aagttaactc gaagtcttag tgcccgtcat atccaaatga tcgcactagg
    17041 tggaacgatc ggtgtcggtc tgtttatggg tgcctcctca accatacgtt ggacagggcc
    17101 ctcagtgatg ggagcctatg cgattgcagg attctttttg tatttgatta tgcgtgcttt
    17161 aggcgaaatg ctttacgttg atccttcgac tggttctttt gcaaattacg cgagcgagta
    17221 catccatcct gtggctggct acttgaccgc ttggagtaat atttttcaat atattgtcgt
    17281 aggaataagt gaagtgattg ctgtaggatc ttatatgaat tattggtggc ctgatttgcc
    17341 ggcaattatt cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc ttatttctgt
    17401 caaggcgttt ggcgaattag aattttggtt ttcgatcatc aaagtcataa cgatcatttt
    17461 gatgatcatt gcaggtcttg gggtaatctt atttggattt ggtaatcatg gtcaggcagt
    17521 agggatttca aatctttgga aaaatggtgg attcttcact ggaggcgtca aaggattttt
    17581 ctttgcatta tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg gaatgactgc
    17641 tggtgaagca gagaatccta aaaagacgat cattgaagct gtccagtcca ctatcggtcg
    17701 aatcttgatt ttttatattg gtgctatttt tatcattgtg agtatctatc cgtggaatca
    17761 attgagtgag attggttcac cttttgtaca aactttttct aaagtcggca ttacatttgc
    17821 tgcaggactg attaattttg tcgtgattac agcggcatta tcaggttgca attcagggat
    17881 tttcagcgca agtcgtatgg tctatacact tgccataaat ggaaagatgt ctaaaaaatt
    17941 tctgaaacta tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct caggtggaat
    18001 cttaattggt ctgatcttaa attatcttct accaatgttc atcaaccagt cagaaaatat
    18061 tttcgttttc gtatacagct caagtatttt gcctggtatg gttccttggg tagtgatttt
    18121 gatcagcgag attcgcttca aaaagaagca cgcagataaa atgcatgatc atccttttaa
    18181 aatgccacta tctccttata cgaattatgt gacacttgct tttttagcta tcgtattgat
    18241 tttcatgttt atcaatcctg aaacgagaat ctcattgatt attggactcg ttttcttgct
    18301 ttacatgacg atccattact ttgtacgcga aaaaagaacc ggaaaataaa aaaagaacga
    18361 agcttcacct tagtggaagc ttcgttcttt tttattgctt ttatcaattc aattttagcg
    18421 tctcaccggg agtcaggaaa taattctcca tgctcatccc attcagcgac aagatcgttt
    18481 ctactggtac gccagttcgt tcggctattt gatttggacc ttctccttcc aaaactacgg
    18541 ttgtcgcagc ctgtgaagtc gcgctgttgg tattagtttc agaggaatct gttgtgtctt
    18601 gtgttgttcg agcttgcatt tcagaagctt ctaccgactc tgctgtctca gaatcagaag
    18661 atgttgtgtc ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc tgcaaataaa
    18721 tatcggaaat cttagcgaca tgattcgtat aattagccga taattgattg atcaaagtat
    18781 ctaagtctac accgtcttta ttttctgctg cctgatacgc tgcacgtaat tttctgatcc
    18841 cttcgtcgga gattgcttga agctcacgtg ctttttggtt agcaactgct gataaaccag
    18901 ctattccctc ttgatttccc tgaatttctg tctggtattc ttcaatcaat gcaggcgtct
    18961 tttcttcgat ctttttcgaa taatcattta cgattgcttg aagttgctct ggcaaaccat
    19021 ttgctgtttt ttcttcagaa gtttgtgtct cttttgtttt agttgatgac tcacttgttt
    19081 gtttcgtctc tgttttcttt gtcgttgttt ccgttatggt cgtacttttc gcttctgacg
    19141 aattttttcc atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa aacacggcaa
    19201 ctgctgcccc aatcatgatc atccgcttac gagctgcctg tttttgctgt cttcttcggg
    19261 tcatccgaac attttctccc cactcatttt catcatacat ttttttcacc accaattttt
    19321 atgtataata gcgttatcaa acgcttcata ttcttattgt agcaaataat tgtcgctgtg
    19381 cacattttta tctttgttat ttataagaaa atttcaccag ttgagatgat tccaagattg
    19441 tccgtcgatt tctccttgca gctgcattgt tcgttcgtct atctcagcca tcagtttctt
    19501 tatttcatat aagagcgcac ggtcttgata ttctgatttt tcgcttaatt catctaattg
    19561 ttggatcagc caatcatttg tcataatgaa gttcctttca tttcttgcag tgtaaaggtc
    19621 aagtcttcga tctgctcttt tgcctcagtc agtgcttcta cgatatatgc ttgtttttca
    19681 gattcttctt cctgcatttt ctgattttcc tgtactcttt gaataaacca gtcaatccgc
    19741 tgagtcagcg atggtttgcc ttccaatgag agatactgct gataagcaat tagcttctcc
    19801 caacgttttt cctgggataa atacataaat tgtgctaaaa cgaatggcgg aagataggtc
    19861 atatggaatt tatttgcgat tcgctgatac ggcctcaaaa attcggaaat cgtatattct
    19921 tcttttcctc cagcttggta ttctttttcc gccacacctg ttgtcaatac gattcctagt
    19981 tctttccctt ttaatacatg tgctgctcgt tccaagacat tatcttgcca aatttttaaa
    20041 tgagcaggag aactatacca gtaaaacgga aactggaaaa tgatccgatc atgctgggcc
    20101 aagagctgta gttcagtctc tatatctatt tttccgtctg gataacttga ttccaaatga
    20161 tgccaagtag ctggtaaacc agcagaagct tctttgaaaa attgctgatt taaagattct
    20221 tcgatcgttg gatggctaat gatgataagt gtacgcatcc cttttcctcc tttttagtct
    20281 tcgctttaca gtgtagcata accgccacaa gtttcttttg ccgatgcttg tcattttaac
    20341 cagactaatc aggttgtcca agaaactgcg gtttgttttt ccaactgttt tttcaaggta
    20401 tccaaagcaa tattcttaaa attttcgttt ttcatacgtt tacgatagtt cgtagccgtt
    20461 tctccggtac tctttttgaa cattcggcta aatacgacta gactattgaa ccctacttga
    20521 taggcaatac tttccacgga ttcatccgtc agagaaagaa gaaacgcccc tcgattgatc
    20581 cgtaccccga tcaaatattc cttgatactg gtacccatat gatttttgaa tactcgcgac
    20641 aaatggctcc gattgaccgc taaatgctgg gcgatttcct caacagacaa tttttcagca
    20701 taatgtgcct cgatgtattt gactgtttca acagctaaag aggaatgaac cttttgtaca
    20761 ccaccgctga cctttccgcc atcttttaaa aggactgata aaaaacggta ggttaatgct
    20821 gttaactgta attcattttc caaggtttct tccgtatctt tcatgcattc taaaataatg
    20881 tcgatatatt tttgtatgtc agaagagacc atcgtgtatt ggtcttctct gaaaagcgac
    20941 tgccgaatga tttcttctgc agcactccca ccaaatgaaa gccagcaata catccacgga
    21001 tcttctccat ctgataagta aaacgtcgag tctcctggac gaatcaagaa cagatcacct
    21061 tttttcaatt ggaattgttg atttttcaca tgataagatc ctttgccttc catcacgaca
    21121 tgcaggatgt aatcattgcg aatagcagga ccaaaagaat gaaacggcat cgttttagag
    21181 aaaccacaaa aatcaaaata caattctttt tctacttgtt gatgttcctt ttttaaaaaa
    21241 atcgattgct ccacgtctat ccctccttac tcctttagta taatgcaaca aaaagttaaa
    21301 taaaagtcac agaaatatat taaaaaggaa gcgatttctt gttaagttta agtcattaaa
    21361 agatctttgt tattcgttgt aaccggttta cttagtatta tatattgaac aggagaggaa
    21421 tacagattat gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc gcttttggac
    21481 acgacacatt ctatgttaca ttaagcactt attttatggt attcgtgaca agccagatgt
    21541 tcgcaggaac agataaggca acgaatgcaa aaatgattgc tacagttaca agcttagttg
    21601 taggtatccg cttagttgaa attatttttg atcctttgat tggtggcatc atcgataata
    21661 ctcgtacaaa atacggtaaa ttcaaaccat ggttagttat cggcggcgtt gttagttccg
    21721 tactcttggt agcaatcttt accaatttct ttggcttagc tactcataat acgactttat
    21781 ttatcatatt atttgcaatc gttttcatct tattggatag tttttattca tttaaggata
    21841 tcgctttttg gtcaatgatt ccagctttga gtacggaaac aaaggaacgt gaaaaatttg
    21901 ctacgtttgc tcgtttcggc tcttctctag gtgcaaatgg tactactcta gtcgttgtcc
    21961 ctatcgttac tttctttacc tatcttgtta ctcataaaca tattgaaggc gcttcaggct
    22021 ggttctggtt cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt atcactgcct
    22081 tgggcacaaa agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct gggatcaaag
    22141 atgtgttcaa agcagttgct caaaatgatc agttgatgtg gcttgccctt gcttatatcg
    22201 cttatgcgat tgccaatgca gcaacaactg ccgtattatt ctacttcttc aaatatgtac
    22261 ttggccaacc aactgaattt tggatcgttg gtctagttgc tgccttactt ggtttagtag
    22321 ctgttccact attcccaatg cttacacgcg tcatctctcg tcgctacgtt taccttggag
    22381 ggatcgcaat gatgatttct tcttactttt tctttacgat cgcaggttca agtttgccaa
    22441 tcgttatcat cgggctagta ttgttctact tccctcaaca attgattttc ttatccgctt
    22501 tgatgacgat cacagactct gttgaatatg gtcaatggaa aaatggtatc cgtaacgaag
    22561 ctgttactct atcaattcgc cctttactag ataaaatcgc tggtgctttg tctaacggga
    22621 tcgtagggtt tgtcgctatc gcttctggga tgactggtaa tgctactgct gctgatatca
    22681 ctgctcatgg cgtaacgact ttcaaagctt atgcattcta tgctcctgct gctttgatga
    22741 ttttctccgc tattattttt gcttggaaga ttacattgac agagaaaaaa catgcacaaa
    22801 tcgttgagaa attggaaaac aaattggctc cagctgatac aatggaagat taacttcacg
    22861 atgcagtagt tccttcccat taagaaagga tcttgaaaat gaagattaca gaaaaagatt
    22921 ttggccaagg ttatcacttg ataaccttgg agaacaaaaa caagctagca ctttctatct
    22981 ctgatttagg tgctagaatc gtcagtttaa aatcaaatga tcgagaatta gttttgggat
    23041 ttgacaccgc tgaagaatat atcgaaaaag atccgtatat cggtgcttct atcggtcgca
    23101 ccgcagggag aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac caattagcca
    23161 cagatccaaa aactggacat aatctgcatg gtggtgctcc tggatttgaa ttaaaaaaat
    23221 ggtcttatgt cattttgaat ggcgaaaatg aagcttctgt catttttact actacaagtc
    23281 ccgacggaga acatggtttt ccaggtacaa tggatgtgga aatccgctat actttgacaa
    23341 aagataatat ctggcgagtg acctctcgcg gcaccagtga ccaagatact ttattcaatc
    23401 cgacaaatca tgtttacttc aacttgacgg gagatgcttc tcagtcaatc gatcaacatg
    23461 agctttggct caatagcgaa gcttatgctc ctttgcgaac agatagtatt cctatagggg
    23521 taaaagaaaa tgctgcagga agtgctttcg attttcaaat acctaaaaaa ttagcgtccg
    23581 tttttgctag tgatttggat caaaaaaatc ttgttgacgg aatcgatcat ccatttttct
    23641 taaaagaaac cggcttgggt aaagaagccg ctcgcttgac tagccccgat aaaagaatcc
    23701 aagtagatat tgctacagat gcttctagtg tggttatttt cactgcaaat ttcggcacag
    23761 aaacaccaga aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca tttgagactc
    23821 aaacagctcc aggtgcagag cgcttctcag catttggttc gattcattta aaagccggat
    23881 cagtatttga aacagtaaca gaattcaaaa tcaaaacaag aaaggaataa ctcatcatgg
    23941 aaatcaaaag aagcttaaac acaaaatttg ccgaaacatt cggacctaaa aaaactacac
    24001 aatacttctc accaggacgc atcaatttga ttggtgaaca cacagattac aatggaggcc
    24061 atgtcttccc cgcttccatc acttatggaa cgcaaggtgt agcagcacct cgagacgata
    24121 ataaaatatt agtttattcc acaaactttg acgatatcgg tgtcattagt tttactttag
    24181 atgagctaga atatgataaa aaagcaaact gggcaaatta cgtaaaagga atggtcctta
    24241 aattaaaaga agctggacac tcaatcgatc atgggtttga gttattagtg gaaggaacaa
    24301 tccctaatgg tgcaggactt tccagtagcg cttcattaga attattagta ggtgttgtat
    24361 tagaagatct gtttgattta aaaacagatc gcttggaact agtccaaaca ggtaaaaaag
    24421 tagaaaatga attcatcggt gtcaattcag gtatcatgga tcagtttgcc atcggcttcg
    24481 gtgagctaga tcatgcaatc ttattagata caaatacgct gaaatacgaa atggttccag
    24541 tgaaactaga tggttatgca atcgtcatca tgaatacgaa taagcggcgg gaattggcag
    24601 attcaaaata caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt ttgcaaaaaa
    24661 ctttgcctat ctcttcttta ggagacttag atgaagaaga atttgaatca actattgacc
    24721 aaatcggcga tgatacattg atacgcagag ctcgccatgc cgtttctgaa aatcagcgga
    24781 ctttgaaggc aaaagctgaa ttagaagctg ggaacttaga agcatttggt caactgttaa
    24841 atgattctca ccactctctt cgctatgatt atgaagtaac tgggatcgaa ttagatacac
    24901 tagtagatgc tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca ggagctggat
    24961 ttggtggctg tgcgattgct cttgtcaaag aagaaaatat acctgccttc aaaaacaatg
    25021 tctatgatga atacatgaaa gttgtcggct atgctccgga attttatgtt gcacacattg
    25081 gaaatgggac aacaaagatc gatccagaag ctgttcatgt ttgataaaaa aatcaacgat
    25141 ctcagctaaa aacaaataga taaaaataac ctctcagcaa tttccaaatg tgagcctttt
    25201 gctcgtattt gaaaattgct gttttttatt gacacggaaa gagaacgtgt gttctaattg
    25261 tgttgaagag gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt atgaaaaaga
    25321 accttcaaga gatattttat gtattgactg caaatctttt tacgcctccg tagagtgcgt
    25381 tgaaaaggat cttgatcccc ttacaactaa attagtagtc atgtcctatc cgtccgattc
    25441 gacagaaaca cggggtagcg gattaatctt ggcttccagt cctgcagcaa aaaaagctta
    25501 cggcatcacc aatatcagcc gtgccagaga tcttcccttc ccctatccaa gtgatttata
    25561 tattgttgcc ccaaggatgg cctattatat ggaaaagaac agagaaatca attctatcta
    25621 taaaaaatat gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa gcttccttga
    25681 tgtaacaaat agcatggcac tctttggtaa aacaaatgcg tacgacatgg ctaaactgat
    25741 ccataaagat gtgtttgagc agaccggaat ctacacgact ataggaattg gcgataaccc
    25801 gctacttgct aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa tgtttgctga
    25861 atggcgttat gaagatgtac cagaaaagtt atggaaaatc tcagatatca ctgacttttg
    25921 gggaatcggc aaacgaacag caaaacgtct gaaacaaatg ggtatacatt ctgtttatga
    25981 tttagctcac gcggattatt atcacttgaa aagcaagcta ggggtcatcg gtactcagct
    26041 ttatgcccat gcttggggca tcgatcgaag ttttttggga caagaatata caccaaaatc
    26101 aaaaagtatc ggaaatagtc aaatcctaga tagagattat acaagacgag aagaaatcga
    26161 gatcgttatc aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat ctgcagcaaa
    26221 aacacaagta gtcagcttat gggtaggata ctctttaggc tatcttgatc caaatggcaa
    26281 gaccgggttt caccagcaaa tgaaaatcga agcgacaaat gctagcagag atcttgcaga
    26341 tgctttattg accattttcc accagcatta ccactaccag gatattcggc gtgtaggcgt
    26401 gaactgttcc gacctcgtat acgcgacagg tttacagctt aacctttttg aagatcctga
    26461 aaagcagata aatgaagcga aagttgattt tgttgtcgat acgattagaa aaaaatacgg
    26521 ctttcaatca atcatccatg cgcatagtct cttgcaagga ggacgcgcca tcgcacgtag
    26581 ttctctcgtt ggcgggcatg caggtggtat gagtggatta gaaggtggaa aagatgagac
    26641 ggacagcaaa aaaattcatt gactataacg agtatcgaga tcggccattt ggattaaaat
    26701 gggggactgc ttttgcgatg gaagaactag tcaacggtat caaaaaaatg aagaagaagc
    26761 acgcaaacag cctcttccta aacaacaaat gacattgcaa gaaatcgata gtatactgac
    26821 agaagccttt ttatctcgta aagcagtaat cattcagctc aatttaaaag atgaatttgg
    26881 aagactcttg gatgaggtcg aagggcactt cattggtgaa gcctatgaag attactttat
    26941 gattgaccat cagccgatat tgtgggaaga cgtacggtat attgagttaa aggaaacaaa
    27001 aaaatggttt gtgacatgag aggttgtgac agaagtgatc aacttcaaaa aataagaaag
    27061 gatttttcga aaattgcttc ttaaattttt gtggaatttt ggcttatttt tcgaagaagc
    27121 aacttctgga acaacgtata ttagatatca gaggttgtga aaaagtcgct tagttttgag
    27181 taacaagcca aggcgctgat ttcacaacct ctgtctatct atccatctat tcggtaagat
    27241 tcatgcatga cgtcttcttt atttagtatg tagagccctt tttcttctga ccattcaaca
    27301 taaatcagct gttttacatc tgcatagcct tctttcttca agttctccat aagccatgct
    27361 tcagtcaatt gattggactg aagcacttta tgctgaattt gcccttcatc tacaagcagg
    27421 atcgataaag ctttgtcttc atctgatttt ttcaatacac tcatttgacc ggcattctct
    27481 aggataacat gtttggcatt ttcaagaaaa aagcattctt gctgtcttaa gattgctcgc
    27541 aactgctcca tctcgatatg gttatttgtc aactctgtca tgttgatgac gccgtctttg
    27601 attagaacag atggctcgcc tttgacccaa cgattgacct tttcattttt ttggacgata
    27661 cgttcgatac cataaatcat tactgcccaa agcgcaatgg caaataggac atgccctaca
    27721 tggacattgt catcataggt tgattcttct aagatcccac ctaaaacaag cgtatagact
    27781 aaatcaaacg gtgtgatttc tgacatggat ttcttcccaa gcaaacgtac cactaataat
    27841 aaaccgatca acccgaacac cagtttcatt gtaattgaaa aaatcatttt ttctccccct
    27901 aactatctgt taattaaatt atatatgctt tctcacgaac acgcatggaa tatacaagga
    27961 agaaatgtat ccttttacta aaaaaaggac tgtgacactg tagtgacccc caaaagttag
    28021 acttttttgg agtggagaaa tccactcctt ttttttatgc tctaaaatga tatttttaca
    28081 caaccgttac ggttgtttta cgaaattgga cgagactttg ccagtttaat ttctgtttta
    28141 tccgttcatt attgtagtaa tagatatagc tatcgatggc tgattttaat tcctcgaaac
    28201 agttataaac tttcccgtga aaaatctctt gttttaataa gccaaaaaaa ttctccataa
    28261 gtgaattatc taagcagttc cctttccgag acatgctttg aaagattttg tactccttca
    28321 atttgtttct ataagctttc atttgatacg cccaaccttg atctgagtga aacgtacgac
    28381 gatagggaca atcgttagtg gtctggatag cttcctccaa accttccatt accgctaatg
    28441 cattgggctt ttcagagata cgataggata aaatctcact attgaacata tccataaaag
    28501 gatctagata caatttcttt tgacggataa tgcctttggc atccacttca tggtatttaa
    28561 attcagttgt atccgtcgtg attttctgat ggcagatact ggtgtagaaa cgacgatgga
    28621 t
//
LOCUS       DACJGB010000033        28240 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.033, whole genome shotgun sequence.
ACCESSION   DACJGB010000033 DACJGB010000000
VERSION     DACJGB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.033"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(130..825)
                     /locus_tag="IYI34_001484"
     CDS             complement(130..825)
                     /locus_tag="IYI34_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAR0559356.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IYI34_001485"
     CDS             complement(915..2015)
                     /gene="ychF"
                     /locus_tag="IYI34_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAR0559357.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(2024..2215)
                     /locus_tag="IYI34_001486"
     CDS             complement(2024..2215)
                     /locus_tag="IYI34_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAR0559358.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(2236..3126)
                     /locus_tag="IYI34_001487"
     CDS             complement(2236..3126)
                     /locus_tag="IYI34_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAR0559359.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(3113..3880)
                     /locus_tag="IYI34_001488"
     CDS             complement(3113..3880)
                     /locus_tag="IYI34_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR0559360.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IYI34_001489"
     CDS             complement(3892..4605)
                     /gene="rsmG"
                     /locus_tag="IYI34_001489"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAR0559361.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IYI34_001490"
     CDS             complement(4837..6738)
                     /gene="mnmG"
                     /locus_tag="IYI34_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAR0559362.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IYI34_001491"
     CDS             complement(6755..8152)
                     /gene="mnmE"
                     /locus_tag="IYI34_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAR0559363.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            8496..8834
                     /locus_tag="IYI34_001492"
     CDS             8496..8834
                     /locus_tag="IYI34_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559364.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(9002..9760)
                     /locus_tag="IYI34_001493"
     CDS             complement(9002..9760)
                     /locus_tag="IYI34_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAR0559365.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(9778..10596)
                     /locus_tag="IYI34_001494"
     CDS             complement(9778..10596)
                     /locus_tag="IYI34_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAR0559366.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IYI34_001495"
     CDS             complement(10593..10949)
                     /gene="rnpA"
                     /locus_tag="IYI34_001495"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAR0559367.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IYI34_001496"
     CDS             complement(11067..11201)
                     /gene="rpmH"
                     /locus_tag="IYI34_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAR0559368.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            11725..13059
                     /gene="dnaA"
                     /locus_tag="IYI34_001497"
     CDS             11725..13059
                     /gene="dnaA"
                     /locus_tag="IYI34_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAR0559369.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            13257..14387
                     /gene="dnaN"
                     /locus_tag="IYI34_001498"
     CDS             13257..14387
                     /gene="dnaN"
                     /locus_tag="IYI34_001498"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAR0559370.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            14616..14858
                     /gene="yaaA"
                     /locus_tag="IYI34_001499"
     CDS             14616..14858
                     /gene="yaaA"
                     /locus_tag="IYI34_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAR0559371.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            14845..15969
                     /gene="recF"
                     /locus_tag="IYI34_001500"
     CDS             14845..15969
                     /gene="recF"
                     /locus_tag="IYI34_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAR0559372.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            15966..17912
                     /gene="gyrB"
                     /locus_tag="IYI34_001501"
     CDS             15966..17912
                     /gene="gyrB"
                     /locus_tag="IYI34_001501"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAR0559373.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            17932..20403
                     /gene="gyrA"
                     /locus_tag="IYI34_001502"
     CDS             17932..20403
                     /gene="gyrA"
                     /locus_tag="IYI34_001502"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAR0559374.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            20639..20938
                     /locus_tag="IYI34_001503"
     CDS             20639..20938
                     /locus_tag="IYI34_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAR0559375.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            20988..21500
                     /gene="ssb"
                     /locus_tag="IYI34_001504"
     CDS             20988..21500
                     /gene="ssb"
                     /locus_tag="IYI34_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAR0559376.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            21526..21762
                     /gene="rpsR"
                     /locus_tag="IYI34_001505"
     CDS             21526..21762
                     /gene="rpsR"
                     /locus_tag="IYI34_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAR0559377.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            21905..23878
                     /locus_tag="IYI34_001506"
     CDS             21905..23878
                     /locus_tag="IYI34_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAR0559378.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            23884..24336
                     /gene="rplI"
                     /locus_tag="IYI34_001507"
     CDS             23884..24336
                     /gene="rplI"
                     /locus_tag="IYI34_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAR0559379.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            24610..25977
                     /gene="dnaB"
                     /locus_tag="IYI34_001508"
     CDS             24610..25977
                     /gene="dnaB"
                     /locus_tag="IYI34_001508"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAR0559380.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            26757..28049
                     /locus_tag="IYI34_001509"
     CDS             26757..28049
                     /locus_tag="IYI34_001509"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAR0559381.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         8598 a         5528 c         5616 g         8498 t
ORIGIN      
        1 tttatcgtaa aaagggactg tgacaagact tttgtcacag tcccttacta ttatttaact
       61 atggataaac aaaatgaggt tctttactac ccctagatat ctagcaaaat agctgatctt
      121 tttgctagtt tatcttcttg ggacggataa acttccaata atcccgtatt ttttcttcaa
      181 gtagtaacgg attgctaaag cagttgcccc gataatgata actactactg gatctaatac
      241 tggattgata actggtggca atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt
      301 cgttccacct aggataagca atgtcttacc cattcctggt ttcttgcttc gatctgctcc
      361 gggatattca tattgataga tgtatttgta catcatataa aagacccagc caccgattgc
      421 agaacctaga acgagtgtag aaaagccata agctggcgtt gttccacgtg aaaacaatcc
      481 ggtaatcgca atcatgatac ttaatagacc aagcaacagc aacgtattat ctaaccacat
      541 caataaaggg gtggatgaat tttttacttc tgctggctta ttgatgattg cttccgttct
      601 ttcagagact gtcccaaaca attgacgggc tgttcttccg cctttttgtt ctgcaactaa
      661 agtaggcaga atgtcatgaa gggcttttac tttttcttct tcagacaaat tcgctgcttc
      721 taaagatttt ttcaaatcaa aaatgtactg ttgatttctt ttggttaatt ttgtttctaa
      781 actattgttt tcttttacga ttgctcgtaa ttgttctggt tccattatct aatagtcctc
      841 cttagccgtt ccttttctca acggtcgtca caaaagacat tctaccatat tctacagcta
      901 gtttgatagc ccttttacac attaaagcgg aataacatta cgtcgccgtc ttgtacgaca
      961 taatcttttc cttctaatcg aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa
     1021 tgatccaagt cttcaaaaga gacagtttct gcacggataa atccgcgttc gaaatctgaa
     1081 tggatgattc ctgctgcttg tggtgctttg attccttttt tgaatgtcca agcacgtact
     1141 tcttgttccc cagcagtaaa gtaagtagct aagcctaaca aatcataagc agcacggatc
     1201 aactgatcca aacctgattc ttcgattcct aaagcttcta aaaattccgc tttgtcttct
     1261 tcatctaatt ctgcgatttc ttcttctgca cgagcactga ccacgatcac ttctgcattt
     1321 tcttctgcag caaagttgcg cacttcttgt acataatgat tcgcgtctgc atctgctacg
     1381 tcatcttctg agacattggc tacataaaga attggttttg tcgtcaataa aaatagtgat
     1441 ttaacgattt tttgttcttc ttcagtaaat tcgatcgttc gggcagattt tccttcttcc
     1501 agtacaggtt tgattttatc cagtacagct agttctgcca ctgcatcttt atctttggtt
     1561 ttagcaactt ttgctacacg agtatagcgt ttgttgatag attctaagtc tgctaacact
     1621 agttccaagt tgatcgtatc aatatcttct agtggatcaa cacgtccttc tacgtgagta
     1681 atattatcat catcaaaaca gcgaacaacg tgacaaatag cgtctacttg gcggatatgg
     1741 cttaagaatt gatttcccaa accttctcct ttacttgcgc cttttacgat tccagcaata
     1801 tctgtaaatt caaaagtcgt cggaacagtt tttttcggat gtacaagttc tgttaagcgt
     1861 tgcaaacgcc agtcaggtac ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga
     1921 tagtttgctg cttctgctcc tgcttttgtg atcgcattaa acaaagttga ttttcctacg
     1981 ttgggcaacc caacaattcc agcagtcaat gccataaatt tattcactct ttctctgcat
     2041 ttttttcaat tacttttttt aatttctttt caaaatctcg acgggtcatc atgacgatat
     2101 gtccgcaatt catacatctg attttgatat ctgcgcccat tcgaatgact tcccagcgat
     2161 tcgtctggca agcgtgaggt tttttcattt caacgatatc gtgcaagtcg tacataaaaa
     2221 tagcctcctt tttgtttatt cttcatcaaa ttgaatattc aagatatcaa gaattcgagt
     2281 caaatctttt tgagaaagat attcaatctc gatcttacct ttcccatttt tttcttggat
     2341 agctacactt gtaccaaatt tatccatcaa acgatcttcg ctttctcgga tataatatgg
     2401 tttttccttt gctagccttg ggattttttt cttttctttt ccatcatttt gatttaactc
     2461 agaaacaagc tgttccagtt ggcggacagt taaattttca gttactgcac gattggctaa
     2521 tttcaagatc aatgttttat tttttaaacc taacagcgta cgggcttgtc ccatagataa
     2581 acgctgatct tgtaccattt ccttgacaag atccggtaaa gaaagcaagc gcaaataatt
     2641 ggcgatataa ggtctgctct ttcctaaacg actagctact tctgcttgtg ttagtttcag
     2701 gtttttcatc agcatctcat atgcttctgc ttcttctaac ggattcagat cttctcgctg
     2761 caagttttcc agcactgcta cttgcatcat cgcttcttcg tcaaagtctc taacgatagc
     2821 agggatcgtt tctttttcag ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat
     2881 ctcatatcct tttactgcgg aacggcgtac gatgatgggt tgaaagacac ctgattgttc
     2941 aatagaatta gctagttcct gcaaagatac ttcttcaaat gttttacggg gctgatacgg
     3001 atttggtcgc agctcattca atggtagctg gacaacggta tcatctttta catccaccgt
     3061 ctctaagttt tctaaatctt gaaacagtgc atcgattcct cggcctaatc ctttactttt
     3121 cttcacgact taccacttcc tttgccaatg cttggtagac ttcagctcct cgagaacgtg
     3181 gatcgtagtc gatgatcggt tttccatgac taggtgcttc tgataaacga atatttcttg
     3241 gaatgatcgt ttcgtaaact ttttcgcgga agtatttacg tacttcttct acgacttcat
     3301 ttcccagatt tgttcgtgca tcatacattg tcagtaagac tccctcgatc tctaattctg
     3361 gattaaagtg tttttggact aaacgaaccg tattcagtaa ttggcttaat ccttccaatg
     3421 cgtaatactc acactgaaca ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg
     3481 tcagatggcc aagagaaggc gggcaatcga tcaaaatata atcatactga ctgcttactt
     3541 ctgctaaaga tccttttaat cttgattctc ttgccatcat tgacgtcaat tcgatctctg
     3601 ctcctgctaa ttggagagtc gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag
     3661 ttgcctcatc aattggcaat tcattgacta gaacatcata gatgtctctt gtcacatcag
     3721 gttttctgat cccgacacca ctggtagcat ttccttgtgc atccatatcc actagcaata
     3781 cttttttgcc aaggctagca agacaagcac ctagattcac cgtcgtcgtt gttttgccga
     3841 cgccaccttt ttggttcgca acagatatta tttgtgccat ctcttctcct cctatttgat
     3901 tggctgctta ttaggcagtc cgggctttct tgggtatttc tttggggttg gtttcttttt
     3961 ttgaataacg atgatatgcc gttgatcaga gacattcggc aaggtaaccg cctcttcttt
     4021 gaggaattta ccacctaata aagcaatcgc ttgttttgct tctgtcaatt cctcttcact
     4081 ttttgctgct ttcaatgcat aaaaatagcc ttcttgttta gccaagggca aacacaattc
     4141 cgctaacaca ttcaaacgag caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa
     4201 gtgaggattt tgcccaaatg tttctgcccg gtcatgatat aaggaagcat cgattcctaa
     4261 taatcgggtt aactcagata agaaggttat tcgtttattc agagaatcaa cgatcgttat
     4321 ctgcagttct ggaaaggcaa tttttaaagg tatgctagga aaccctgccc ccgaaccaac
     4381 atcgcataaa gataaaggtt gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta
     4441 aaaatgcttc agatatacgt ctttttcatc cgtaatagct gtaaggttca tcttttcgtt
     4501 ccattcgact aatgtccgat aataaatagc gaattgattc atttgatgat cggtaagctc
     4561 tatccctttt tctgccaagg attggcgaaa ttcttctggt atcataaata ttcctttcat
     4621 tgtgaaacag caattgtttc acagattctc tccctacttt aacgcaaaat ctctagacat
     4681 gcaatccttc catatggatt cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt
     4741 ctttctagta tgactcataa aatatacaca gaagagagaa ctagctttga gcaatttctg
     4801 caaaaactag ttctctcttc tatttattat tacgtattac gctggttcac tgactttcgc
     4861 aatcttccct tgttcgatat agaccattag gatacttagg tctgcagggt ttacgccact
     4921 gattcggcta gcttgggcaa ttgtttctgg ttggattttc tgcaattttt gttttgcttc
     4981 tgttgctaat ccgttgattg ctgcgtagtc gatgttttct ggaatctttt tcgcttccat
     5041 gcgttttaat ttttctactt tttccatggc ttttttgatg tagccttcat atttgatttg
     5101 gatttctact tgttcgattt cttttcgtgt caattcttca ttttctggaa taaaccgtag
     5161 aagttctgca taagtaatct caggacgtct taaaaagtcg cttgctagta caccatcttt
     5221 taatggtgcc gcattaatcg ttgctaaaaa gtcttggact tcttctgttg gtttgattcg
     5281 tacattcatc aaacgtttga tttcttgttc aactgcttct tttttcttca aataattctt
     5341 gtattgatct tcttttacta agcctacacg gtgacccatt tctgttaatc gcaaatctgc
     5401 attatcatga cgtaaaagca gacggtattc agcacgtgaa gtcagcaaac gataaggttc
     5461 gttcgttcct tttgttacca aatcatcgat catgacaccg atgtaaccgt cacttcgttt
     5521 gatcactaaa ggttcttttc cctgaacttt caaagctgca ttgataccgg caatcagccc
     5581 ttgtccagcc gcttcttcat aaccgcttgt accatttgtt tgtccagctg tataaagatt
     5641 ttcaacgact ttagtttcta atgtcggacg gagctggtgt ggctctacta cgtcatattc
     5701 gatagcatat cctgtacgca tcatttctac tttttctaat ccagcaatag aatgaagcat
     5761 gttgacttga acatcctctg gtaaagaagt agataaacct tgaacataga cttcttccgt
     5821 atttaagcct tctggttcca aaaataattg atgacgtggt ttgtctgcaa aacgcacgat
     5881 cttatcttcg atggatggac aatagcgagc gcctacccct tcaacgatcc ctgtaaacat
     5941 tggggcacga tgtaagttgt ctcgaataat ttggtgagtc gtctcacctg tataggtcag
     6001 ccagcaagat tcctgatcta gtttataggc actatctggt gtgctgaagc taaaatgatt
     6061 cggctctttg tcaccaggct gctcttctgt aacagaatag tcgatcgtac ttgattttac
     6121 tcgaggcggt gttcctgttt tgaaccgttc gatagcaaag ccaagttctt ttagatgatt
     6181 cgctagtcca atagaaggtt gagagttatt cggacctgat gaatatttca attcaccgat
     6241 aatgatttct ccgcgaagtg ctgttccggc tgtaataaca actgctttgg cccggtagct
     6301 cgctcctgtg gacgtcacta ctccatgaca aacaccatct tcgacgatca gttcttcgac
     6361 gattccttgt cttagcgtca aatttggttc tttttcgatc gtatgtttca tttctgttga
     6421 ataagcatgt ttgtctgctt gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt
     6481 caacatacgc atttggatat aggttttatc gatattctta cccatttctc cgcctaacgc
     6541 atcgatttct cgaacgacaa cgcctttagc tggtccgcct acagaaggat tacatggcat
     6601 gaatgctacc atgtccaaat ttattgtcag taataatgtt tttgatccca tacgtgcagc
     6661 agcaagtgca gcttctgaac cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc
     6721 tgcttcataa tgttccaatc gtttgttcct ccttttattt tcctaaacaa aattgactga
     6781 ataattgagt gattagctca tcttgtacac tgtcgccgac aacttctcct agatagtccc
     6841 agcaacgcgt catatctatt tgaacgagat ccacaggcat tcctgaatca atacctgcaa
     6901 tgacttcttc aagtgagagg gaagcttttt ctaacaacgc gatatggcgg gtattagaaa
     6961 cataagtagc gtctctttct cctgtttctc cgctgaagaa taaatcagaa atagctgatt
     7021 ccaaacgatc tagtccgtcg tttttagcta cggaaattga aaaaactggt tcattttcga
     7081 tcaatttctt caatttttct tgttccagct gtgctggcaa atctgtttta tttagaagaa
     7141 tgatccgttt caatccactt gtcgcttcca ataattgttc atcttctgct gtcagtggtt
     7201 cgctttggtt tagaacaagt aagatcaagt ctgcttctgc taatgctttt cggcttcttt
     7261 cgaccccgat ccgttccacc acatcttctg tttcccgaat tccagctgta tcgattagct
     7321 tgagcggtac cccccgaaca ttgacatact cttcgattac atctcgagtc gttcctgcaa
     7381 tatccgtaac gattgctttt tcttctctta acaaatggtt taacaaactt gatttgccga
     7441 catttggccg gccgatgatt gccgtactta acccttcccg cagaaccttc ccttgcttgg
     7501 atgtagcaag tagcgcttgg attctttgtt ggatcatctg cgctttttca agcaataact
     7561 tcgtagtcaa ttcttcgaca tcatcatatt ctggataatc aatatttact tctacttgtg
     7621 ctaatgtctc taaaatctct tgtctcaatg agcggatcaa agcagacaga ttgccgtcta
     7681 actgattaag agctagtccc attgctttgt ctgtttttgc ccgaatcaaa tccatcacag
     7741 cttctgcctg tgaaaggtca acacgaccat tcaagaatgc tcgttttgtg aattcgcctg
     7801 gctcagctaa tcttgcacct tctcttaaca atagctgcaa aatttgattg actacaacga
     7861 tcccaccgtg acaattgatt tctactacat cttcccgcgt aaatgtcttt ggtgctctca
     7921 tcacggagac catgacttca tcgacaagct gattgctctt gggatcaacg atatgtccat
     7981 aatggatcgt atgacttggt acttctgata atcgtttgtt tccagattga taaactttat
     8041 ctgctagttg cacagcttga tcgccactta aacgaacgat actgattgca ccttctccag
     8101 gtggggtaga aattgccgca atcgtatcaa attctaaagt aatttcagcc attttttcac
     8161 ttcctttttc attttgcgca caaaaaaagt gcccactcca ccctttatgc acaataaacg
     8221 gtgaacttcg cactttgact gcttgttctt gattaaattc tggtcataga ttatcacact
     8281 tgctataagg aatcaagtgt ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat
     8341 taccttatat ttaattgtcc ttggatgtct ctttgcgttc tttggtttat tcgaagagaa
     8401 gatactgtat aaacaaggct ttatctttct taaaaaaaac ggtacactaa atggtagcgc
     8461 tatctaaact atacactttt gaaaggagaa aatatgtgag cccgttcaga tctgctgcat
     8521 tggaagaaga aagtgaccct cggatcaacg atgtcgtttt atctacaggg aatatcaacc
     8581 agaaatatat cgtccgcgat atcgtttttg ttgtggaatg cttagaagct gatttgtttg
     8641 acccatcttt tcccaaagat gaccttttct taacaacgaa gaggaaatta aagacgaagg
     8701 tacttgaata tggtgcgaat gcagtaatca actgtcattt tgagtatcag caaaaaaaac
     8761 gcgacggaaa gtattttctg gaactttttg cttatggaac agtgatccaa tttatccaaa
     8821 caacgattgg ttaaatgaaa cagacaaaaa aagcggatca aaactgaaga cagtttcgat
     8881 ccgctttttt ctatcaaagt tagtgactac ttcatcgtat actctgataa gaatgatttc
     8941 tagtaaaata tacttggttt gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga
     9001 atcaataata ttttttagca ggttctacta ctaaataacg ataaggttcc tcaccttccg
     9061 aatgtgtttt tacatgttca tctttgctta atgcagcatg gatttgttta cgttcaaatg
     9121 caggcattgg ttctaagaaa acaggctgcc ctgtccgttt tactttttca gccgtgcgat
     9181 cagctaaacg ctgaatgatc tcttggcgct tttcacggta attgcctaca ttgactacta
     9241 cagacagctt atttcttgct aaacgatgga tgaagacttg tgccagatat tgcaatgcat
     9301 tcagtgtttt tccatgtttc ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa
     9361 caaccatatt atttgattct ctttccattt tgacaagagc tggagcatca agctgttttg
     9421 aaatttcagt aagataaact gccaattccg ttaaagctgc ttcatcacct agctcactta
     9481 cttcttctgt tttttcttca agaatcgttt cgttgactac ctcgatctca gctgttggtt
     9541 cttcttccaa aatttcttct acagtctgtt ctactgtttc agaaactttt tcgctgatac
     9601 ttggctggat cgaaagccgt gcattctttc gacccattcc taggaaacct ttttttcctt
     9661 cttccagtat ttcgatttcg acttggtctt tggccaaacc caaatcagcc agacctagtt
     9721 ggacagcttc gtctattgtt gcagcttcat aaatcggcat tagcattacc tcccctatta
     9781 tttccgtttt ttcttaggat tcattgcttt cttaagagca cgttctcttt cgcggattct
     9841 ccgtgcttct tcttcacgct cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg
     9901 tacaacttgg aaggcattgg aaactaccca gtataatgaa agaccgctgg ctaaattgac
     9961 acccatcact aaaatcatta caggcatcac gaaattcatg atcttcattg atgcattcga
    10021 ttcaatttga ctcatgctag ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat
    10081 cggcaaaata aatgttggat ccgcgttccc taagttcaac cataagaaat gcccttgttt
    10141 caactctggt acacgtgaaa tagattgcca taacgccatc aagattggca tttgtactaa
    10201 taaaggaaga caaccagcat atggattgac gccattttct gcatataaac gctgctgctc
    10261 ttctcttaac ttactttgtg tttccggatc tcttgaacta tattgttgct gcagtgcttt
    10321 taatttcggt tgtaattctt gcgttttacg catgctcttt gtttggaaat gcatcaacgg
    10381 caataggatc acgcggatga tcagtgtaaa taaaatgatc cctatccctg cattaccaaa
    10441 tgacaacgcc ttgatcgcct cagcaaagta ataaacaata taacgatccc aaattcctgt
    10501 actctgtgcg ctgacttccc cagttccgca accggataag acgatgacca aactaacgat
    10561 accggccatc aaaagtaaac gtttgtattt cttcacttac tcttttcctc tctgtcgatt
    10621 atttttgcaa gttttaagac gtggactaaa ttggagcgaa cctcatcata agtcaacccc
    10681 ttcacacttg gtctagcaat cacaataaaa tccaactccg gatcaatttc ttcttttata
    10741 gaataaactg ctgctcggat ttttcgtttg acttcattgc ggtttaccgc gttgccgacc
    10801 ttttttccta cagataaacc aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa
    10861 acgacgaatt tgcggttagc aaaagaatct cccgtatgga acacggcttg aaactctcgt
    10921 tcttttttta ctcgatacgc ttttctcatt agttttcttt ccttttctat ctctcactta
    10981 cgctcataat cataccataa ttacgattgt cctttcatca tttccctaaa aaagagtata
    11041 aaaaaaacca ctgaaacccc agtggcctaa gctgaaagaa cttttcttcc tttgcgacga
    11101 cggctagcta acacacgacg tccattttta gtactcatac gtttacggaa tccgtgaact
    11161 ttttgacgtt tacgtttatt tggttggtat gttcttttca tatattccac ctccaaaatg
    11221 ttcttatcta ttttataaac acagacatac tttgatagta taacgacaaa gtagtgattt
    11281 tgtcaatata aaatagaaga tcgattgtta aaacttatcc acaagattat ttttttctat
    11341 gggagtccta tgtggaaaaa gttatgcaca gggattatcc acagataata aatggctggt
    11401 tttacacaga aaacacacgt gtaaaaaaag taatccacaa tcaggtgatt tcctgtgtat
    11461 tccgtctcct aactattggt atatctatat tttttatcca cagatgcaag aggaaatcta
    11521 ttgattttta cgagttttcc acctgtgcat tagtggtgtg gaaaactttt ttttgacaac
    11581 tgtgtttttc tcttttttct ttttgtcgat tgtgtggata attgtaatta aaaaattttt
    11641 ttcttcggtt tctgtggaaa actattgaaa aaaatgctaa attagtacta gttattcact
    11701 ttttaatgaa ggaggaggcc ttatatggta tccctcgatg ctttatggaa tgaattaaaa
    11761 gcaacatacc aaaaggattt atcgcctgct agctataata catggatcga aacagcgaac
    11821 cctcgaaccc ttgatcaaaa ccagctcgtt gttgaggttc ctagcaagat tcataaagaa
    11881 tattgggaaa aaaacctggc gacaaaaatc gttgaagtcg gttatatgtt atcaggaaat
    11941 gagattatcc ctcgctttat tactggcgaa gaagcagagc aagaagaagt tatagaagaa
    12001 aaaaatccaa aagttgtggc acctagtccg ctaaaaaaag ccatgctgaa ccctaaatat
    12061 acctttgata cgtttgtcat tggtaaagga aaccagatgg ctcatgcagc agcgctcgtt
    12121 gtagcagaag atccaggttc tatttataac ccgctcttct tttatggagg cgtgggactt
    12181 ggtaagactc acttgatgca tgcaattggt catcaaatgc tgcaaagtca gcctaatgcc
    12241 aaagtaaaat atgtaagcag tgaaactttt gctaatgatt tcatcaattc gatccaaaac
    12301 aaaacagcag aagagttccg tcaagaatat cgaaatgttg atttactgct agtggatgac
    12361 attcaatttt ttgcagaaaa agaagctacg caagaagagt ttttccatac tttcaatgcc
    12421 ttgtacaatg aagggaaaca aatcgtacta accagtgatc ggttgccaaa cgagattccg
    12481 aaactgcaag aacgcctcgt ttctcgcttt gcatgggggc tgtctgttga tattacaccg
    12541 ccggatcttg aaacaagaac agcgatttta cgtaaaaaag cggcggctga acgtctagag
    12601 atcccagatg acacactaag ctacatcgca ggacaaattg attctaacat tcgtgaatta
    12661 gaaggtgcgc ttgttcgagt gcaagctttt gctgcgatga atagtgaaga tatttcgact
    12721 agtctagctg cagacgcact gaaaacgtta aaatcaggaa aaggacatcc tcaattgtca
    12781 atcctgcaga tccaagaaga agtggcaaag tattatcaca tccaattgaa agatatcaaa
    12841 ggaaagaaac gcgtcaaatc tattgttgtt cctagacaaa tcgctatgta tttggcacgt
    12901 gagctcacag ataattcttt accgaaaatc ggagctgaat tcggtggcaa ggatcataca
    12961 acggttatcc atgctcatga aaaaatccag cagctgatgg attctagtgt cagtatgcaa
    13021 aatgaagtat cagaaatcaa aaacttttta ctaaattaat ttgtcgattc tatcaactga
    13081 aaatagcatg tggataaaag aaaaaaacgt tcaaaagtta tgcacacttt ttccacagct
    13141 gttttttcgt ttctctctta cgttttttga gttttccaca gaatcaacat gccctattac
    13201 ttttattatt ctttttaact aataataaat aaacaaacat tcaaaggaga tacatcatga
    13261 aagttacttt aaaccgagct agctttatgc aggaattgca aactgttcaa cgagctattt
    13321 caagcaaaac cacgatccct attttgacag gtgtaaaaat cacactgaca caagaaggtt
    13381 tgactttgac ggggagcaac gctgatatat caattgaaac ttttttgtct gttgaaaacg
    13441 aaaaagcaaa tatgcaaatc gaatctactg gttccattgt tttacaagca cgtttcttta
    13501 gcgaaatcat tcggagactt cctgaagaaa catttacttt agaagtttta gaaaataaac
    13561 aagtagcgat tacttctgga aaagcgaatt ttatcgtaaa tggattagat gcagataact
    13621 atcctcatct tcctgttgtc gaaagccata accagatgaa attacctgta cacgtattga
    13681 ctaaactaat caacgaaaca gtttttgctg tctcacaaca tgagagtcgt ccaatcttga
    13741 caggtgtgca tttcatttta tctgataatt ctttattggc tgtagctact gattctcacc
    13801 gtctaagtca acgcgtgatt ccagtagaac aagcggctga tcattttgat attgttattc
    13861 ctggaaaaag tttgatcgaa ttatctcgct cattaacaaa tgaagaagaa atcgtcgaaa
    13921 tcagcattat ggaaaaccaa gtgttgttca aaacagaaac gatgtatttc tattcccgtt
    13981 tgctagaagg aaactatcct gataccaatc gtttgattcc ttcaagtttt aatacagagg
    14041 ttgaattttc tgttcctagc tttttagcgg cgatcgaacg ggcttcttta ctttctcatg
    14101 aaggccgtaa caacatcgtt cgtttatcta ttcgtccaga tgcggttgtt ttatacggaa
    14161 attcgccaga aatcgggaaa gtagaagaaa gcttgagtta tacagcaagc agtggcgatc
    14221 cattagatat ttctttcaat ccggattata tgaaagcagc gttgcgtgca tttggagata
    14281 tgagcatcaa agtgaaattt atctctgcta ttcgtccatt tacattggaa ccaacggaag
    14341 atggcgttca atttattcag ctgattactc ctgtacgtac aaattaacaa tgattttcat
    14401 tgttcttgaa aatttaattg aaaagaaata gaaaaaataa aattttaaac agaaggccct
    14461 aaaatctctt ttaaggcttt ctgtttattt ttgtttaaat cacctttttt agattaaatc
    14521 gtcttatatg gaaaaatata tggataaatt tgttttattg cgaaaaaaaa ggtataatag
    14581 agtatacttt taataatgaa aatgagggat gggcattgaa acaacaagtc attctaaata
    14641 cggaattcat gacgttgggc caaatgttga aagaagtcac agttatcggt agtggcggac
    14701 aagcaaaatg gtatttggca gaaaacactg tgttagtcga tggcgaacca gaaaatcgtc
    14761 gtggacgcaa actttatccc ggcatgatgg ttgaagttcc agaagtagga acttttttta
    14821 tggtaaaaaa aggaaacgac gataatgagg ctgaataagc tgtatttaaa aaattatcga
    14881 aattacgaag agctgaacat cgaattttcc aagaacctcg tcatcttttt aggagaaaat
    14941 gcgcaaggaa aaacgaatct tttggaaagt atctacgtat tagctatgac gcgaagtcat
    15001 cggacaagta gtgagcagga attgattggc tggactgacg atcaggcgat gattcaagga
    15061 gagatcacaa aaggttcatc cactcttcca ttagaaatct tattatctaa aaaaggccga
    15121 aaaaccaaga tcaaccatat cgaacaaaaa aagttaagta cttacgtggg acaaatgaat
    15181 gtcattttat ttgcaccaga agatctttct ttagtaaaag gcagcccgca gatcaggcgg
    15241 aagtttttag atatggaatt aggccagatc aatccaatct atctttatga tcttgtccaa
    15301 taccaagcaa ttttgaaaca aagaaaccag tatttaaagc aattgaatga aaaaaaacaa
    15361 actgatttgc tttatttgga tattttgtcc gaacaactag cagcgtttgg cagcaaagta
    15421 ttgaaagcaa gagcacagtt cgtcaagcga ttggagtttt gggctaattc tttgcatcag
    15481 caaatcaccc atcaaaaaga gcagctggaa atcgagtatc tcaccgcagt cgattcttta
    15541 gaaacacata cgcaagagca aatccaagaa caatttctcg cacttttgaa ccaaaataaa
    15601 aagaaagacc tttttcgggg aacaacgaca gttggtcctc atcgtgacga tctttccttt
    15661 tttattaatc agaaaaatgt gcagacttat ggatcgcaag gacagcaaag aacaactgca
    15721 ctaagtgtca aattagcaga aatcgatttg attaaagaag agacggggga atatccaatc
    15781 cttttgttag atgatgtgat gagcgagctg gatgatagtc gtcaactcca tctgctagaa
    15841 acaatcgaag gaaaagttca aacgttctta acaacaacga cacttgacca tgtaaaaaat
    15901 aaaatgactg ttgaacctga gattttttat gttcaacaag ggaaactaaa aggagaagaa
    15961 actgaatgac agaagaaaga agtttagtag aacgcgctaa agagtatgat gccagtcaga
    16021 ttcaagtact tgaaggactt gaagccgttc gtaaacgacc tggcatgtat atcggttcga
    16081 ctagttctga gggtctgcat caccttgttt gggaaattgt cgataattcg atcgatgaag
    16141 tattagcagg ttttgcaacg aaaatccatg tgattattga aaaagacaac agtatcaccg
    16201 ttattgacga cggtcggggg atacctgtag atatccaggc aaaaactgga cgtcctgcag
    16261 ttgaaacggt ctttactgtt ttacatgctg gaggaaaatt cggcggtggt gggtacaaag
    16321 tttctggtgg acttcacgga gtaggatctt ctgttgttaa cgcgctttct acacagcttg
    16381 atgtcaaagt atacaaagaa ggaaatgtgt actatcaaga atatcgtcgt ggagctgtag
    16441 tggacgactt aaagattatc gaacaaacgg atcgccatgg aacaacggtt cactttacgc
    16501 cagaccctga aatttttaca gagacaactg agtttgattt ttcaaaattg gctacccgga
    16561 tccgtgaact tgctttcttg aaccgcggga tgcgtatatc gattgaagac aaacgagaag
    16621 aagaaccagt catcaatgaa taccattatg acggcgggat taaaagttat gtcgaatact
    16681 tgaatgcaaa caaaaccgtg atttttccag aacctgtata tttggaaggt gaacagcaag
    16741 atatcgcagt tgaagtctct atgcaatata cagacggcta ccattccaat atcatgagtt
    16801 ttgccaacaa tatccatacg tatgaaggcg gaacacatga atcaggattc aagactgctt
    16861 taactcgtgt gataaatgat tatgcccgaa aacaaaagct gatgaaagaa aatgacgaca
    16921 acttgaccgg tgaagacgtg cgagaaggat tgacagctgt tatttctatc aagcatcctg
    16981 accctcaatt cgaaggacaa acaaaaacaa aattgggcaa ttccgaagta cggacagtca
    17041 ctgatcggtt atttagcgaa catttcatga aattcttgct ggaaaaccca agtgtgggcc
    17101 gtcaaattgt tgaaaaagga ttactagctt ccagagctcg tctagcagcg aaacgtgcgc
    17161 gtgaagtaac gagacgtaaa ggtgctttag agattagtaa tctgccagga aaattagcgg
    17221 actgctctag caacgatcct gaaaaatgcg aattgttcat cgtcgaagga gattcggccg
    17281 gcggatcagc taagcaaggt cgtaatcgag agtttcaagc catcttgcct attcgtggga
    17341 aaatcttgaa tgttgaaaaa gcaagcatgg acaagatctt ggcaaatgaa gagatccggt
    17401 ctttatttac tgctatgggt acagggtttg gagaagattt tgatgtatcg aaagctcgct
    17461 accacaaatt agttatcatg actgatgccg atgtcgacgg cgcgcatatc cgtacattgc
    17521 tgctgactct tttttatcgt tacatgcgtc ctattgttga agctggatac gtctatattg
    17581 ctcagccacc tttatacggg gtaaaacaag ggaagaatat tacgtatgta caaccaggaa
    17641 aaaatgcaga agaagagttg aaacaagtcg ttgcttcact tcctgcctca ccaaaaccaa
    17701 gcgttcagcg ttataaagga cttggagaaa tggatgatca tcaactatgg gaaacaacta
    17761 tggacccaag caatcggatg atggcccgcg tcagtgtgga cgatgctatt gcagcagatc
    17821 agatatttga aatgttgatg ggggatcgag tagaaccccg tcgtgcattc atcgaggaaa
    17881 atgcacacta tgtgaagaac ttagatattt aatcatgaag gaggaaaaag aatgagtgaa
    17941 gaaatcagag aaaacatcca tgatgtcaat ctaaccagcg aaatgaaaga ctccttcatc
    18001 gattatgcga tgagtgttat tgtcgcacgt gcacttcctg atgtcagaga tggtctgaaa
    18061 cccgttcatc gccgtatttt atacggcatg aacgaattag gtgtaacacc ggataaagcc
    18121 cataaaaaat ctgccagaat cgtcggagat gtcatgggta aataccatcc tcatggagac
    18181 tatgcgatct atgagtcaat ggtacggatg gcacagccat tcagctaccg ttatatgcta
    18241 gtggatggtc atggaaactt tggttctgtt gacggtgacg gtgccgccgc tatgcgttat
    18301 accgaagcac ggatgagcaa aatcgctaca gaaatgctgc gtgatatcaa taagaacact
    18361 gttgatttcc aaagcaacta tgatgataca gaaaaagaac cagttgtact acctgctcgc
    18421 ttcccgaatc ttttagtgaa cggaacaacg gggatcgccg taggtatggc cacgaatatc
    18481 ccacctcata acttgtctga ggtagtagcg gctatcgatt tgctgatgga taatcctgat
    18541 gtgacgacca atgaattgat ggaagtatta cctggaccag attttccaac aggtggtttg
    18601 gttatgggaa aatcaggaat ccgaagagcg tatgaaacag gtaagggttc tattaccgta
    18661 cgtgcaaaag ttgagattac tgagatgcca aatggtaaag aacggatcat cgtgacagaa
    18721 ttgccttata tggtcaataa agcaaaattg atcgaacgga tttctgaact gcacagagac
    18781 aagcgtatcg aagggatcac agatttgcgg gacgaatctt cccgtgaagg tatgcggatc
    18841 gtcattgaca tccgtcgtga cgccagcgca tctgttatct tgaataactt atataaattg
    18901 acatccttgc aaaattcatt tggattcaat atgttggcga tcgaaaaagg tgtgccgaaa
    18961 gttttgagct tgaaacaaat tcttgaaaac tatatcgaac atcagcgcga agtcatcaca
    19021 agacggacag tctttgaaaa agaaaaagca gaagcaaggg cacacatttt agaaggtttg
    19081 cgtattgctt tggatcatat cgatgaaatc attgcgatta tccgcgggtc taaatcagat
    19141 gacgaagcaa aagcgacgat gatcgaacgg ttcgatttat ccgatcgcca gtctcaagct
    19201 attttagata tgcgtttacg ccgattgacc ggtttggaac gggaaaaaat cgaaaatgaa
    19261 taccaagaac tcttaaaact gattgaagat ttaactgaca ttctggcaag accagaacgt
    19321 gttacagaaa tcatcaagaa tgaattagca gacttgaatc aacgatttgg tgataaacga
    19381 cgcacagaat tactagtcgg tgaggtctta agtctggaag atgaagattt aatcgaagaa
    19441 gaagaaatcg ttattacatt gacaaacaat ggttacatca aacgtttggc aaataatgaa
    19501 ttcagagctc aacgaagggg tggacgtggt gtccaaggaa tgggtatcca caatgacgac
    19561 ttcgtcaaaa atctggtttc ttgttctacc catgatacat tgttattctt tacgaataac
    19621 ggaaaagtgt atcgggcaaa aggatacgaa attccagaat acgggcgcac agccaaagga
    19681 ataccaatta tcaatttact aggtatcgat tcgagtgaaa gcatccaggc aatcattgcg
    19741 gtgacgggag aagcagaaga aggccactat ctcttcttta cgacgcgtca aggtacagta
    19801 aaacgtactt ctgtcactgc ttttgctaac atccgaagta atggtctgat cgcaattggt
    19861 ttgaaagaag acgatgagct cgtcaatgtg ctactgacag atggtcaatc taatatcatt
    19921 attggaacac ataacggcta ctctgtcact ttcaaagaag aagcagtccg cgatatggga
    19981 cgtacagctt ccggggttag aggaatacgc ctgcgtgagg aagattatgt cgttggggca
    20041 gccttaatga aggaagatca agaagtatta gtgatcacag aaaaaggtta cggaaaacgg
    20101 acaaaagtat cggaatatcc agtgaaaggc cgcggaggta aagggatcaa aacggcgaac
    20161 atcactgaaa aaaatggtcc actagctgga ttgaccactg tttctggaga tgaagacatc
    20221 atgctgatta cagataaagg tgtcattatc cgtttcaacg tatcgactgt ttctcaaact
    20281 ggacgttcaa cattaggcgt acgtttgatg aagatggaag ctgatacgaa agttgtcacg
    20341 atggcagcag tcgaaccaga agctgcagaa caaacagttg aagaacaagg atcagaagaa
    20401 tagccatcaa gactaattgt gtaagctagc aagaatgacc gattaaaaaa tctggttatt
    20461 cctgctggct tttttgatta gttgaatttc tcttaaaata ttcgaaactg ttcattctgt
    20521 attgcttttg accaaaatag agtgtataat cagagcttgt gagtagtcta attaattaga
    20581 taactctcct tgctcttagg aacctaagag ccgaaagtcc acagggaggt gaatatcaat
    20641 ggaaaacacg aaatatgaaa tcatgtacat tattcgtcca aacattgatg aagaagcaaa
    20701 aactgcttta atcgaacgtt tcgatgcaat cttgaaagat aacggagcag aagttatcga
    20761 atcaaaagat tgggaaaaac gccgtttcgc ttatgaaatg aacggttacc gtgaaggtat
    20821 ctatcacatc gttaaagtat cttctccatc atcagcaaat gctgtcaatg aatttgaccg
    20881 tcttgctaaa atcaacgacg atatcattcg tcacatgatt gttaaagaag aagcttaatt
    20941 gttgtttcat gtgaaacaat agaaaacgat gagaggagat gactattttg attaataatg
    21001 ttgtattagt tggacgtctg acgaaagatc cagatttgcg ttacacagca agtggtacgg
    21061 cagtcgcaac atttactttg gctgtgaatc gtaattttac aaaccaaagc ggcaatcgcg
    21121 aggctgattt tatcaactgt gtgatttggc gcaaatcagc agaaacttta gcaaactatg
    21181 ctcgtaaagg aacactttta ggtgttactg gacgtatcca aactcgttct tatgataatc
    21241 agcaaggaca acgtgtatac gtgacagaag tagtagctga caacttccaa ttgctggaaa
    21301 gtcgttctgc atctgaaaat cgccaacaaa gcggcggatt ccaaagttct ggtcaatcag
    21361 ctggcggatt tggcggaaac aataactcta atcaaacatc tcaatcatcc aacggtatgc
    21421 ctgatttcga ccgtgatact tctgacccat tcggctcaag ttcaacgatc gacatttcag
    21481 acgatgattt accattctaa acgaatttga taggagggaa atagaatggc acaacaaaga
    21541 agaggcggac gtaaacgtcg taaagtagac tacattgctg ctaaccatat tgaatatatc
    21601 gattataaag atatcgaatt actaaaaaga tttatttcag aacgcggtaa aatcttgcca
    21661 cgtcgtgtaa ctggtacagg cgctaaaaac caacgtaaaa ttactgttgc aatcaaacgc
    21721 gctcgtatca tgggcttgct acctttcgtt agcgaagact aataaatcct aggaattgaa
    21781 tgactgatcc ttttgatcag ctattcaatt cctttttttc tgtaaaaata acctctattc
    21841 gtttagtcgc tcgaatgacg aaagttatgg taaaatcaaa ttagtcatat atgggaggaa
    21901 aattatggaa aataaaaaca atcatttatc cccgaaacct ctcttcctta tcctgtttat
    21961 attaatcgaa gtattggtag tcttaatttt accatttcgt tttgttagcg cttcggtcat
    22021 tctattgatg aatttatggg gtgttcgcta ttggttgaaa atgaaacgtt ggcttatttt
    22081 aaataaacgt gaaaaaatcc agcaggcttc gactgtagct gaaaccaatc tagcttatgt
    22141 ctcagaagtc atgcctgtag gagtgattgc ttatcagcca tcaagccaaa aaatcgaatg
    22201 gctcaatcca ttagcattga aaatcaaaga tcaaagtgga ttaacagagg aagaattgct
    22261 agatactttt tttactgcaa aagaaaaaaa gaaaaaccaa gtctatctag cagataaaac
    22321 atttcagttt gatatggata cagtaaaggg tgttgtttat tttaatgata ttacccaaaa
    22381 ccagttgtta aaactccatc agacgaacag tcagccggtg attggtattt tatctattga
    22441 taactatgca gatgccattg ataaattgga tgacaaagag atttcttatt tgaactcttt
    22501 cgtcactaca ctagtttctg actggatgaa tgaataccat gtctttttca aaagaatcaa
    22561 cgcagaacgg tttttcttcg tagcacagat gatcgatctg aaaaagatga tggataaaaa
    22621 atttgatttg ctggatcgct ttcgagcaga agctgaaaaa cagagctcgc ctatcaccct
    22681 tagtatgggg attgcttatg gctcaagaac cctcgatgaa atcggtggag aagctcagaa
    22741 taatttagat gttgctttag tccgtggagg cgaccaagtt gtcgtgaaag aggtaaaaga
    22801 aggagcaaaa cctgtttatt atggggggaa atctgctaag aatgtcaaac gcacgcgtgt
    22861 tcgttctcga gcgatgagta ccgctttgcg tcggattttt gatgaaacag gcgatgtgtt
    22921 tatcatgggg cacaagtttc cggatatgga tgctttaggc tctgcttttg gtgtcagttg
    22981 tctggcacgc ttcaaccaga aaaaagctta catcgttatc aacgagaacg agatcattcc
    23041 agatgtagaa cggtgtcttg aagaaatcca taagcatcct gaattagaaa tgcaactaat
    23101 ctctccagaa cgtgccatag agctgaaaaa agaagaggac ctcctcgtga tggtggatta
    23161 ccataaacct tctctatcga tctctcaggg cctatatgag caatttgata agattgtgat
    23221 cattgatcac catcgtcgtg gggaagagtt tccaagtaaa cctctgctga cttatatcga
    23281 atcttcagct tcttcagctt cagagctagt ttctgagctg atccaatacc aaagcagcaa
    23341 aacaaatcgc ttagatcgtt ttgaggccac attattgctt gcagggatgg ttgtagatac
    23401 aaatagtttc aatgtccgta caactgcacg aacttttgac gttgccagct atttaaggtc
    23461 ttgtggagca gacgcatcct tagtacaata tttattgagc tcggatttag cgacatactt
    23521 ggagatcagc aatttgattg ctggaagcga ctatgtgact tctgatattg ttgtggcggc
    23581 agggacagac gaaaaagagt ataatagtgt tatagcagca aaaacagcag atacattgct
    23641 ttcgatgatc aatatcaatg cagctttcgt cattacaaag cggacagacg gcttgattgg
    23701 gatcagtgcc agaagtaccg gcacgatcaa cgtccaaatc atcatggaat ccttaggtgg
    23761 cggaggacac ttcacaaacg cagctactca actggaaaat acgacagttg aggaaacaaa
    23821 acagaagcta tttgctgcaa tcaaagaaaa tatgaatcaa atatatgata aggagtaatc
    23881 caaatgaaag taattttttt acaagatgta aaaggaaaag gtaaaaaagg ggacatcaaa
    23941 gaagttccag caggttatgc acaaaattat ctaatcaaaa ataacttggc aaaagaagca
    24001 acgaaaagca gtatcagtca gttagcagga caaaagaaag cacaagaaaa acacgaagct
    24061 gaaatcttgg aagaagctaa aaagttaaaa gaattcttgg aaaaagaaga tactgtagtc
    24121 gaattgaagg cgaaagcagg ggaagacagc cgtttgtttg gttcgatccc ttcaaaacaa
    24181 attgctgaag ggttaaacaa acaatacaaa atcaaattag ataaacgaaa aatcgaatta
    24241 gaaaatccga tccgttcact aggctacaca aacgtacctg ttaagcttca tcatgaggtg
    24301 acggctacga tcaaagttca tgtcgtacaa gaataacaaa tcttgatagc gatgttcagc
    24361 tggtaagtat aaccagaagg aaaaaagaca aaccgaaatt tatctgagaa aacagctgca
    24421 gcaatgaaag caaaagctgc aaatcgattt cagattgatt tcggtttctt ttttgggaac
    24481 agcaaaatag cataaagaaa acgagtataa taaaagagaa ctattttttt attacacttt
    24541 cggtacgaat gatgactttt ctctcatttt cttgtaaaat gaagcaattg aagacaagga
    24601 gcgatacgta tgaatgaagt gtggcaggat cgaatcccac cgcaaaatat tgaagctgaa
    24661 caagcggttt taggctctgt ttttcttgac gcagaaacga tcattgatgc catggagtac
    24721 atagagccaa aagactttta tcgtcgtggc catcaaatta tttttgagac aatgatcgag
    24781 ttaaatgatc gtaatgaagc gattgacgtg gttactgtca aagaccggct ggaacaagcc
    24841 aatttgttag aagatgccgg tggtttaagt tatctctcag acttggcatt agcagttccg
    24901 acagcagcaa atatcgtcta ctatgcgaaa atcgttgaag aaaaatcatt gctgcgtaca
    24961 ttgatccaga cagcaacagg aatcgttacg aaaggattcg agcaaggaga agaagttcaa
    25021 acaattttag atgatgccga acgcagtatc ttggaggttt cggaaaaaag aaatcgaagc
    25081 ggctttttat ctattgctga agtactgaac tccacgattg aaaatatcga tcgactagct
    25141 caaaacaatg aagaaatcac aggtctacca actggctatc aaggtctgga taaaatgaca
    25201 gcaggtttcc agcccgaaga actaatcatt cttgctgcac gtccagcggt aggtaaaaca
    25261 gcgtttgcat tgaatattgc tcaaaatgta ggaacaaaaa cagatcgttc tgttgcgata
    25321 tttagtttgg aaatgggggc agaatctctt gtaaatcgga tgctttgtgc agaaggttcg
    25381 attgaggcca gccatttacg tacaggtcag ctttcggaag aagaatggtc gaatctgatc
    25441 gttgcgatgg ggagtctttc tcgagcaaat atttttatcg atgatacacc agggatcaaa
    25501 atctctgaaa tccgtgcgaa atgtcggaaa ctagcgcaag aaaaaggcaa tctaggcttg
    25561 atattgatcg attatttgca gttgattgaa ggtaacggta aagagaaccg tcaacaagaa
    25621 gtatcagata tctctcgcca actgaaaaaa ctggcgaaag agctgaaagt acctgtcatt
    25681 gctctttcac agttatctcg tggagtagaa cagcgacaag acaaacgacc agtgctaagt
    25741 gatattcgtg aatccggatc aattgaacaa gatgcggaca tcgtagcctt cttgtatcga
    25801 gatgattatt atgaaagaga aggcggagat gacgatgatg gaaacgagcc aaaaaatgac
    25861 aacgtcatcg aagtcatcat cgagaagaac cgaagcggtg cacgaggaac agttgaacta
    25921 ctgtttatca aagaatacaa taagttttcg tcgctttcgc caagagaaga attttaaaga
    25981 agaggttgtg agccttgagc aatcccgaga gctctcgtaa gaagatgcaa ggagtaaccg
    26041 caggaacgac tagagtactt aatttaaaca gatctccaaa gggattgtga caaaagtctt
    26101 gtcacagtcc ctttttccga ataaacggtg ttcaatcagc tgacccttgg tattagctca
    26161 gcaaacgtca aaatatgagg aactattttg acgtttgttt ctctaatact caggtcaaaa
    26221 cgctgatctt acgacctctt ttccttaaac ggtgttctcc tgccaactcc tcggtcacaa
    26281 gtcacaatat catcaaacat gagaagcagt tttttgaaat tgctccttgt gactcagagt
    26341 taaacggcag gctcacaacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
    26401 tacgcccaat caaacgaaaa atatgagaag ctatttttgt ttggctgttc ttattacttg
    26461 atgcaggaca gctttttcac aacctctgat tcacggtgtt ccaaaagtag cttcttcgaa
    26521 aaataagtcg aaatttcaca aaaatttgag aagcaatttt cgtacattcc ttcttatttc
    26581 ttgaagctga ccacttctgt tacaacctct tttttaataa ccattctaaa aagataaagt
    26641 tcgtttttta agcgttttta taaaataaaa ataaaaatca ttcgatttta tttgaaatat
    26701 gtatctatta ttgatacaat ggttcaggat taaataatat agaacgaggt gttcacatgt
    26761 catctgtagt agtagtaggt acgcaatggg gcgatgaagg gaaaggaaaa atcacagact
    26821 ttctcagtga aaatgctgaa gtgattgcac ggtaccaagg tggcgacaat gcagggcaca
    26881 caattaagtt taacggtgtg acttataaac tgcatttgat tccttctggg attttttata
    26941 aagacaagat cagtgtgatc ggaaacggcg ttgtagtcaa tcctaaatca ttggttaaag
    27001 aattagctta tttacatgaa aaaggtgtag aaacaagtaa tttgcgtatt tctgaccgcg
    27061 cgcacgtaat cttgccttat catatcaaat tggaccaatt acaagaagat gctaagggcg
    27121 aaaataagat tggtacaacg attaaaggaa tcggacctgc ctatatggat aaagcagctc
    27181 gtgtaggtat ccgtattgca gacttattag ataaagaaat ttttgaagaa cgtcttcgtt
    27241 tgaatctaga tgacaaaaac cgtcaatttg taaaaatgtt tgattcagaa ccacttgatt
    27301 ttgacgatat ttttgaagaa tattatgaat acggacaaca aatcaaacaa tacgtcacag
    27361 atacttccgt tatcttgaat gatgctttgg atgcaggcaa acgcgtgttg tttgaaggcg
    27421 cacaaggtgt aatgctggat atcgaccagg gaacttatcc ttttgttact tcttctaacc
    27481 cagtagccgg tggggtaaca atcggaagcg gtgtagggcc ttcaaaaatc aataaagttg
    27541 tcggcgtctg caaagcctat acgtcacgtg tcggtgatgg accatttcca actgaattat
    27601 ttgatgaaac aggagaacaa atccgcgaag taggacgaga atacggtacg acaactggtc
    27661 gtccgcgtcg tgtaggctgg tttgattctg tagttatgcg ccattctaaa cgagtttcag
    27721 gtattaccaa cctatcttta aactcgatcg atgtattaag tggattggaa acagtaaaaa
    27781 tttgtacggc ttatgaatta gatggcgaac taatctacca ctatccagca agcttgaaag
    27841 agttgaaccg ctgcaaacca atctatgaag aattgccagg ctggtcagaa gacattacag
    27901 gatgcaaaac acttgcagaa ctgccagaaa acgcacgtaa ctatgtacgt cgtatctcag
    27961 agcttgttgg tgtacgtatc tcaacatttt ctgtcggacc tgatagaaca caaacaaaca
    28021 tcttagaaag cgtctgggcg caaatctaag gataaaaacg acgactgaat caggacgttc
    28081 ttaccagagc gagtcgaagt gtttatctat acgataataa taacttgaag aggtagtgaa
    28141 agagacgttt agccttattg ccgataggct gtctcttgaa cactgtttat tcagaataag
    28201 ggactgtgac aaaaaccgtg tcacagtccc tttttccgaa
//
LOCUS       DACJGB010000034        26472 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.034, whole genome shotgun sequence.
ACCESSION   DACJGB010000034 DACJGB010000000
VERSION     DACJGB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.034"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(98..1228)
                     /locus_tag="IYI34_001510"
     CDS             complement(98..1228)
                     /locus_tag="IYI34_001510"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAR0559382.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IYI34_001511"
     CDS             complement(1877..3079)
                     /locus_tag="IYI34_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0559383.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IYI34_001512"
     CDS             complement(3139..3342)
                     /locus_tag="IYI34_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAR0559384.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IYI34_001513"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IYI34_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IYI34_001514"
     CDS             4879..5691
                     /locus_tag="IYI34_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559385.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6912)
                     /locus_tag="IYI34_001515"
     CDS             complement(6715..6912)
                     /locus_tag="IYI34_001515"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559386.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="IYI34_001516"
     CDS             complement(7058..7936)
                     /locus_tag="IYI34_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR0559387.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="IYI34_001517"
     CDS             complement(7975..9093)
                     /locus_tag="IYI34_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAR0559388.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="IYI34_001518"
     CDS             complement(9090..11189)
                     /locus_tag="IYI34_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAR0559389.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="IYI34_001519"
     CDS             complement(11240..12061)
                     /locus_tag="IYI34_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR0559390.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="IYI34_001520"
     CDS             complement(12058..12867)
                     /locus_tag="IYI34_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0559391.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="IYI34_001521"
     CDS             complement(12888..13382)
                     /locus_tag="IYI34_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0559392.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="IYI34_001522"
     CDS             complement(13394..13882)
                     /locus_tag="IYI34_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR0559393.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="IYI34_001523"
     CDS             complement(13959..16655)
                     /locus_tag="IYI34_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0559394.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="IYI34_001524"
     CDS             complement(17083..18156)
                     /locus_tag="IYI34_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0559395.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="IYI34_001525"
     CDS             complement(18153..18980)
                     /locus_tag="IYI34_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559396.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="IYI34_001526"
     CDS             complement(18981..19787)
                     /locus_tag="IYI34_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559397.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="IYI34_001527"
     CDS             complement(19799..20884)
                     /locus_tag="IYI34_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559398.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="IYI34_001528"
     CDS             complement(20901..21443)
                     /locus_tag="IYI34_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559399.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="IYI34_001529"
     CDS             21701..22690
                     /locus_tag="IYI34_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAR0559400.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="IYI34_001530"
     CDS             22687..23424
                     /locus_tag="IYI34_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAR0559401.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="IYI34_001531"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="IYI34_001531"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAR0559402.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="IYI34_001532"
     CDS             23988..24620
                     /locus_tag="IYI34_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR0559403.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="IYI34_001533"
     CDS             24705..25352
                     /locus_tag="IYI34_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0559404.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="IYI34_001534"
     CDS             25357..26340
                     /locus_tag="IYI34_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAR0559405.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8102 a         5591 c         4486 g         8293 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat taccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gc
//
LOCUS       DACJGB010000035        26160 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.035, whole genome shotgun sequence.
ACCESSION   DACJGB010000035 DACJGB010000000
VERSION     DACJGB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.035"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(62..1093)
                     /gene="queA"
                     /locus_tag="IYI34_001535"
     CDS             complement(62..1093)
                     /gene="queA"
                     /locus_tag="IYI34_001535"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAR0559406.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1286..2461
                     /locus_tag="IYI34_001536"
     CDS             1286..2461
                     /locus_tag="IYI34_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR0559407.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            2465..3106
                     /locus_tag="IYI34_001537"
     CDS             2465..3106
                     /locus_tag="IYI34_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559408.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            3103..4020
                     /locus_tag="IYI34_001538"
     CDS             3103..4020
                     /locus_tag="IYI34_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0559409.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            4017..4688
                     /locus_tag="IYI34_001539"
     CDS             4017..4688
                     /locus_tag="IYI34_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559410.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(5229..5717)
                     /locus_tag="IYI34_001540"
     CDS             complement(5229..5717)
                     /locus_tag="IYI34_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAR0559411.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(5918..6184)
                     /locus_tag="IYI34_001541"
     CDS             complement(5918..6184)
                     /locus_tag="IYI34_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAR0559412.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(6184..6447)
                     /locus_tag="IYI34_001542"
     CDS             complement(6184..6447)
                     /locus_tag="IYI34_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR0559413.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(6440..6712)
                     /locus_tag="IYI34_001543"
     CDS             complement(6440..6712)
                     /locus_tag="IYI34_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559414.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            6923..7108
                     /locus_tag="IYI34_001544"
     CDS             6923..7108
                     /locus_tag="IYI34_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0559415.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(7250..9073)
                     /locus_tag="IYI34_001545"
     CDS             complement(7250..9073)
                     /locus_tag="IYI34_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAR0559416.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(9671..12376)
                     /locus_tag="IYI34_001546"
     CDS             complement(9671..12376)
                     /locus_tag="IYI34_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAR0559417.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(12682..13629)
                     /locus_tag="IYI34_001547"
     CDS             complement(12682..13629)
                     /locus_tag="IYI34_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAR0559418.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            13819..14088
                     /gene="rpsN"
                     /locus_tag="IYI34_001548"
     CDS             13819..14088
                     /gene="rpsN"
                     /locus_tag="IYI34_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAR0559419.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(14264..14626)
                     /locus_tag="IYI34_001549"
     CDS             complement(14264..14626)
                     /locus_tag="IYI34_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR0559420.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(14637..15758)
                     /locus_tag="IYI34_001550"
     CDS             complement(14637..15758)
                     /locus_tag="IYI34_001550"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAR0559421.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(15774..16124)
                     /locus_tag="IYI34_001551"
     CDS             complement(15774..16124)
                     /locus_tag="IYI34_001551"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAR0559422.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(16254..17765)
                     /locus_tag="IYI34_001552"
     CDS             complement(16254..17765)
                     /locus_tag="IYI34_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAR0559423.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(18087..19439)
                     /locus_tag="IYI34_001553"
     CDS             complement(18087..19439)
                     /locus_tag="IYI34_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAR0559424.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(19592..20668)
                     /locus_tag="IYI34_001554"
     CDS             complement(19592..20668)
                     /locus_tag="IYI34_001554"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAR0559425.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            20864..22609
                     /locus_tag="IYI34_001555"
     CDS             20864..22609
                     /locus_tag="IYI34_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAR0559426.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(22889..23326)
                     /locus_tag="IYI34_001556"
     CDS             complement(22889..23326)
                     /locus_tag="IYI34_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAR0559427.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(23310..24683)
                     /locus_tag="IYI34_001557"
     CDS             complement(23310..24683)
                     /locus_tag="IYI34_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559428.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(25005..25493)
                     /locus_tag="IYI34_001558"
     CDS             complement(25005..25493)
                     /locus_tag="IYI34_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0559429.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(25515..26117)
                     /locus_tag="IYI34_001559"
     CDS             complement(25515..26117)
                     /locus_tag="IYI34_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAR0559430.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         7503 a         5561 c         4838 g         8258 t
ORIGIN      
        1 ctttgtcata aaatctttgt aaagcttttt tgttattcaa aactagcggc ttttcataac
       61 cttatttcac gaacatcgca tctccaaaac taaagaaacg atagcgctct tcaatcgcat
      121 gctggtaagc agataaagtc aattctcttc ctgcaaaggc actaactaac atgactaaag
      181 tcgattttgg caaatggaaa ttagttgaga aagcttctac cactttgaat tcatagccag
      241 gcgtaatgaa aatatcagtc catccgctgt ctgctttgat ttctccatta aatttcgttc
      301 caatcgtttc taatgttcga attgaagttg ttccgacagc cacgattttc ccgccatttt
      361 tacgtacttg gttcaatcgc tcagccgctt cttccgtcaa acggtagaat tcactatgca
      421 tatggtgttc ttcaatttta tctacgctga caggtcggaa tgtacctaac cctacatgta
      481 gtgtcagata gactaattca acaccctttg cttcgatctc atcaagtaat tctttcgtaa
      541 aatgcaatcc agctgttggc gctgcagcag aaccattttc tttcgcatag acagtttgat
      601 agcgatctgg gtcttccaga cgttctttga tatatggagg caatggcatt tctcccaatg
      661 attcaagaat ctctagaaaa attccttcat actcaaagtc aatgatccgt ccgccgtgtt
      721 ccaattcttc tttcactact gcttgcaatc tgccatcacc aaaagagatt cttgtcccaa
      781 ctttggcacg tttcgcaggt ttgatcaatg tctcccacgt atctccttgt gtattcgtta
      841 gaagaaggac ttctaaatgc gcaccagtat ctggtttttc tccatataaa cgagcaggca
      901 gaactcgtgt attattcata actagcgcat caccaggctg taactcttca atgatttcat
      961 ggaaatgttt atcttccatc tctccagttt tccgatccaa aacaagtaaa cgagaatgat
     1021 cgcggtcttt taatggtgtt tgtgcgatta attcttccgg caagtcaaaa tcaaaatctt
     1081 ctgttgttaa catatcgtca ctcactttct atcatgtgct tcatcattct atcatgtttt
     1141 tcgttctgtt ggcaaaatca gcactttttc acttctattt tttgttttcc taaataaaaa
     1201 aactacccta tttgtatttt ttcattgttt taggcatact agtaatagat agttcttcat
     1261 tgaaaataga aaaagaggtg gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa
     1321 aggtggaaaa gtcgctgtag aggacgtcaa tctttcattt gaaaagggtg agttcatttg
     1381 ttttatcggt acgagcggaa gcggaaaaac gacgacgatg cggatgatca accgaatgac
     1441 tgatccaaca caagggaaaa ttttgattga tggaaaatcc atccaagaaa ttgatccagt
     1501 ggaattgcga cgtcagatcg gctatgttat ccaaagtatt ggattgatgc ctcacatgac
     1561 gatccgtgaa aatatcactc ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa
     1621 taaaacagct gaaaaaatga ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta
     1681 cccacatgaa ttatctggtg gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc
     1741 gaatcaagat atcatcttga tggacgagcc attcggtgcg cttgatccaa tcactcgtga
     1801 ttccttgcaa gaccttgtca aagatttaca agagcgtttg ggaaaaacga tcgtatttgt
     1861 aacccatgac atggatgaag cgattaaact agcaagccgt atcgccatca tgagtgaagg
     1921 ccgtgtgatc caatttgata caccagaaaa tattttacgt catcctgcaa acgatttcgt
     1981 agaagagttg attggagaag atcgcctact acaagccaag ccaaatgcga caagagtcgg
     2041 cgaagttatg ctaaatacag caatcacgat tacaccggaa aaatcattac aagaagcaat
     2101 caaactgatg agggaaaaac gtgtagatac gttgttagtt accgataatt cgaatgtatt
     2161 aaaaggattt atcgatgtag aaacgttgaa taaaaaacga ggaaaagtat ccagtgtcgg
     2221 cgacattttg aataaacatg ttttttacgt aaaagaatcc gcctatcttc gtgatacctt
     2281 gcaacgcatt ttaaaacgcg ggctgaaata cgtgccggtc gttgatgaac agaacaaagt
     2341 cgtaggtatc ctgactcgtg catcattagt ggatatcgtc tacgatgtga tttggggaga
     2401 agatcccgca actactgatg ttgcagagtc agctactcct gaagaatcta aggaggtctg
     2461 acctatgcag cagttttttc atcagcacgg ttcagaaatt ctctcaaaag gttttgaaca
     2521 tctttatatt tcaggacttg ctttgatcat tggtatcatc attgctgtac cgcttggtgt
     2581 cttactgaca cgttttccaa aagctgccac gattgtgatt ggcttaacca gtgccttgca
     2641 gacaatccct tctcttgctt tattggcatt gatgatcccg ctatttggcg taggtaaatt
     2701 acctgcgatt atcgcactct ttatttattc attattgccg attttacgta atacgtacat
     2761 tgggatgaaa aatgtcgatt ggaattatcg ggatgtggca aaaggcatgg ggatgactga
     2821 gttccaatcc attttttccg ttgaattgcc cattgctatg ccaacgatca tggctggtat
     2881 tcgtttagct gccgtttacg tgattgcatg ggctacactt gcctcttata tcggcgctgg
     2941 tggtttaggc gatttgattt ttagtggact gaataattac caaccagatc tcattttggc
     3001 aggtacgatc cctgtaacaa ttttagcact attggctgat tggcttctag gtctcttgga
     3061 acaccggctg acaccgatcg cacttagaga ggagaatgaa gaatgaaacg tatactaaaa
     3121 atgaccatgg ttcttttctc tcttcttata cttgcgagtt gttccttccc tggtcttgcc
     3181 agcaatacag atgaagacac catttcgatc acaggtggaa tcacttcaga agcacagatc
     3241 ttagcaagtc tagttgccgg aatggttgaa cattatacag ataaaaatac cgcaatcatc
     3301 aacaatctgg ctactacaac tatcaaccat caagcgatga tgaacggaga tgcttcaatt
     3361 tcagcggctc gctacacagg aacagattta acgactacat tgaaccttcc tcctgaaaaa
     3421 gatccgaaaa aagcgtttgc taccgtgaaa gatgaatttg agaaaagata cggtcaaacg
     3481 tggttcccgt cctatggttt tgaaaatacg tatgtctttc ttgttcgaaa agacaccgca
     3541 caaaaatacc atctatctaa agtcagcgat ctgaaaaatg tagcggatga attagtagct
     3601 ggtgtagata cttcttggat caatcgtaaa ggagacggtt atgacggctt ccaagaaact
     3661 tatggcttct ccttcaattc gatattacct atgcagattg gacttgttta tgacgcagtc
     3721 gaagcaggaa aaatggatat tgttcttggc tactctacgg atggacggat cgccagttat
     3781 gatctagtca tgttggaaga tgatcgtcaa ttcttcccac catatgatgc tgctccagtt
     3841 gtagacaaca agatattagc tgatacgcct catttaaaag aagccttaaa taagttaggt
     3901 cataccatca gtacggaaaa aatgcaggaa ttgaactacg aagcagataa taatctagtg
     3961 gaaccatcag tagtagcaca acgtttctta caagaaaacc actattttga agggaagtga
     4021 agcaattgga taaaatgaat gtctttcaac aatttttcta ttatttccag gaaaatggct
     4081 cctatatctt tgcacaattt atccgtcact tcctgatttc tatctatggt gtcctctttg
     4141 cagcagttgt cgggattccg gttgggatca tgatttctag gagacgtaaa ttagcaaact
     4201 gggtgattcg tttggcgaat atcatccaga cgatcccttc tctagccatg atctccattt
     4261 taatcattgg attaggcctg ggagtcaatg tcgtgattgt tactgtattt ttgtattccc
     4321 tcttaccgat catcaagaat acgtataccg ggatgatcca agtagacaaa aacatccttg
     4381 atgtcggcaa aggcatgggg atgactgcta gacaacgact attcatggta gagttaccac
     4441 tctctgtttc tgttatcatg gcaggcataa gaaacgcgtt agttgttgcc atcgggataa
     4501 ccgcgatcgg tgcattcgtc ggtgctggtg gtcttggaga tatcattatc cgaggaacca
     4561 atgcaacaga gggcacatcg attattttag caggtgcatt gccaactgca ttaatggcga
     4621 tcatcacgga ctggctttta ggtattttgg aaagacggct agatccagcc agccgtacat
     4681 cacgctgatg ttattgtaaa aaacaaaagc ttctgcgacc atttgttggt cgcaaaggct
     4741 tttttagttc tgtcattttt aaggcaataa tccactctga ttcttccctc cttatgggca
     4801 aagagttgct tttcttagct atttatccat tccgtatact ccaacttcat actgccaacg
     4861 ggttcatatc tgcatccacc gactaagttt ttttcttatc cgctaaggca ttttgccaat
     4921 ccattttccc atcactcgtc atggaattca taccacatga tctctaaata ctcgtctgct
     4981 tgttctttga attgggcatc aattcttaca tcctttggcg tggctttcat ttaagccttt
     5041 tccatcagtc ctcgattgtt cgatgccttc agctgcatca tcaaaactgg tgactgccgt
     5101 atcggtaact tcgatcatct tttatacaat cgggaacttg ccatcctcat ccttggctaa
     5161 aaacatatct agccattcca gcttctcgcc atgggaggtt tgcggttcct aaaaccggta
     5221 cacgattctt atggactgca tccgtcgcaa ctagacttgg cgatacgatg atccttcacc
     5281 agaagagcac ccccgtaaac caattgatcg atatattgcc aataagaaaa gacattggcg
     5341 taaaagatat cgatccctcg tgatgcattg ccgttgatac tgctattcat ggccgaaagc
     5401 gccacgactt taacttcttc cgtttgagtg acattcatgt tcggcaatgt cgctttggat
     5461 acccgctctg ctaaaggaac gacactggta ttgtatttcg tatctgtatc ttttccaaaa
     5521 gaccatgcca acgaatcctc tgggaaccaa taagacgact ccggttgatt gtccattgct
     5581 tttttcaaca tacttttggc tgtttctcga taaacgacat ttccaattgt cttcgcctca
     5641 ctttggcgat aatcgatggt cgttgccact gccaacacta acaacgatgc tccaatttta
     5701 ctgattttcc ttgtcacact tcttcctcct tgttttacat aaacccgtca acattccatt
     5761 cctaaactca aacaatgacc cttcaaaggt ggagatatag gaacaaatta aagaaatcat
     5821 gcaataaatc caagaaggtt ggttttagaa caaaaaaaga gtctcctttt tggggagact
     5881 ctttgttaac tatctaaaaa ggttggctct tctcacttca ctcataatgt gtccaggcag
     5941 aataaatttc tacaacttta ttttcctcat caactttgta aaccactcga tgctgtctat
     6001 tcagcctgcg tgagtatctg ccttcatgtg taggtctcag cttttcaaaa gactgcgtgg
     6061 gcatatatgg atcttccttc aatgtctgaa tcacttcttc aaattgtttc tttaaattcg
     6121 tttgcttgat ttttcttaaa tccactttcg cagagttttt gattgcgact gtatagttac
     6181 tcatcaaaga gcttcccaat caatatcatc gatattcgta aagctactgt cgtctttttc
     6241 gcggtctcgt actttatcta aagtacctgt ttgctcaaga aaaagggttt cttgaatcgc
     6301 tctccagtct tccagcccaa tcaatacagc attgtttcca ctacgatcac taataatctc
     6361 gatttcagta tgattttcat ttacctcttt caataactga taaaaatttt tacgagcagt
     6421 gctaggattc atcgttgtat tagccattgt ctctcctcct ttttctgata taattatagc
     6481 gtacttgatt aagtacgtca aaaaaataca ataaagcact taaattcagc aaacttttcc
     6541 tcttttttac atagagaaaa agaccccttt ttagggagga tctttgtcca cgctcacttc
     6601 ttactccttt acgactagcg gtagccaaac gtaaagtaat tggtaacaac gtatcatctt
     6661 tagcaaaggt cgaatccaaa gcctgtgagt ctatgcaaca acaccaatac acgaaggttc
     6721 ctcgttgcag tgtgacttga ttgatatctt gaataacctg aggatacgca cgaaagattt
     6781 gtaacggtta ttcaaaactg attttggccg tcaagtcttc tactgcctca aaaatgattg
     6841 cctctttcgt ctcctgtcat tcatgttcac tatcgatttg atacttgctt agaccgttgg
     6901 gtttgtgaat caccaattaa aaatggcact gagttatggt aacaaaaaat ggtcacttga
     6961 cgaggctgag attcctactt atgaagacac atggcactta agagtcgagg taaaggaaga
     7021 agctgaaaat ataggaggct gtacattatt tggcaaagga ttcggaaatt atgatcaaga
     7081 catcaaaatc aaaatgtttt acagctaaat aggaagaata ataacatttc atacgctgtt
     7141 cccatcttta atggcttata gagggcacct ttagctgaga ataagacaaa acagaccctc
     7201 tagcttcaat taagagctag agggtctgtt tttgatttta aaacttggtt tatttcttca
     7261 aatggtagct ataggtgctg acaacgatat tttcacgcca agacaatcgc aatcttaacc
     7321 gcaaacttgt ttggttatgg agaatattct gccagcgtct gtttggtaca aattgaggaa
     7381 tcaacacagt agttgtatag ttttgtttcg ctgcattttt acttacgata tctacataac
     7441 ggataattgg attttcaatc gagcgataag aagagtggac gattgaaaaa cggacatctg
     7501 ggaaatgctt tttaaattct gcttcgattt ctttttcttt ttcgacatct tcgtctagcg
     7561 aaacatgcat cgcaacgaca taatcaccga tcgaacgtgc gtaattcaac gcaccgatat
     7621 ttacccgtgt cacattcccg actaatacaa gaacggtatt cccgtcataa tcatgtagtt
     7681 ttgcatcatt ttctaaacga agctgttctg ctactttttt ataatgatca tggatcttat
     7741 aaaaaatgaa cataacgatt ggcataatga tgaagaaagg ccaaatgtcc cctaaccgat
     7801 agacaaacaa aatcgcaatg attgcatatg agatgaacgc acccgtaata ttggcaatcg
     7861 atttgcttaa ccatcttttt ccttcttttt tccatttgac gaccatccct gtttgagata
     7921 aagcaaatgg aatgaacacg ccgatagagt ataacggaat caagcgttct gttgagccat
     7981 ggaaaataaa cagcaatacg atcgaaccta gcgctagtgt gataatccca ttggaatagc
     8041 ctagtcgatc cccacgatct tgatacatgt gaggcatgaa tttatctttt gccagattgt
     8101 atgcaagaac tggaaaagca gaaaaaccgg tgttagcagc aactgctaaa atcagcgctg
     8161 tagcaaattg aaggacgtaa tacaaaatac catgtccaaa gacagcttta ccaatttgag
     8221 acaaaacagt gacttctttt tcaggaacga tcccatacca atagttgata aatgtgatcc
     8281 ccacgaagaa gaaacctaag atcaatgcca tcatcgtcaa tgtagcagcg gcattttttg
     8341 ctcttggctt cttaaagaat ggtacagcat tactaatcgc ctccacccca gttaaagagg
     8401 aagaaccaga agaaaatgct cgtaaaatca aagcgataga aacacccggc acagccgctc
     8461 ctggaagtgc agtagcttgc aatggcacag cacctgtcac gatattatat aaaccaacaa
     8521 cgatcagtaa cgagatcaca aggataaatg tgtacaccgg aaataatagg aacgatgcag
     8581 attccctcag cccacgaaga ttcagcatca tcagtagcaa aacgattgtc acagaaatag
     8641 cgacttgatg accgtagagt gctggcaccg ctgatgtgat cgcttcagca cctgctgaga
     8701 cagatactgc tactgtcaac atgtagtcga tcaataaaga gccaccggaa atcaagccag
     8761 cattctttcc aagattctca ctgctgacga catatgctcc cccgccatga ggataagcat
     8821 ggatgatttg acgatatgat agcgttaatg aaatcaataa aataataaca aaagcggcaa
     8881 tgggtagcga ataccagatg gctgcagcag acaaagcgac taaaacgaca acgatttgtt
     8941 ccgtaccata tgcaatagat gataacgcat cagacgacaa taatgccaaa gcagcaaatc
     9001 gggttaattt gtgctcatca ttttcagcag attttaaagg cttacctact aacaaccttt
     9061 ttaagtaatc caatttctaa actcctttac acacaatttt acttcaagtt ttatagtcga
     9121 aaatttatag taaacaagat gttatagtac gctcactgac cttaaaagtc aataaacttt
     9181 aagaaaataa ttggaaaaaa acagaaaaat atcaagcgct ttttcaaacg ctttaataca
     9241 gcaaaaatct aacatttttt tatgggcttt ttctttgaat tctataataa aaggatagaa
     9301 cttgacttag aaaatcaagt tctatccttt ttgatatctt aacttttgga agagagatgt
     9361 aaggctgctt tctacccatc attcttacta ctcgaagcat agcgcagttg tcacaacctc
     9421 ttgataaacg gtgtttaaaa gcttgcatct gcggtaataa gcccaaccaa attaaaaaca
     9481 taagaagctg tttttatttg gtcgttctta ttacttgatg cagagcagct tttttacaac
     9541 ctcttggtac acggtgttca atcagctgac cctcggtatt agctcaaaaa aacggcaaaa
     9601 tatgaggaac tatttttcca tttttttctc taatactcag gtcaaaacac tgatttcaca
     9661 acctcttttt ttaatgaaaa atatggctct catgcgccgc ttctttgaat ttcgtcattt
     9721 tttcttcgat gaacggatag attgccgttc ctacgattcc aaagacaaca aacaaaccga
     9781 tcatgatcca gattgctttc gtagcatttg gccaataaat tcctcctgca gattcccgta
     9841 acaagtttac cgcatgagta aatggcaaga acggattgat catttggaag aatttccctg
     9901 agacttggat cggatagttc ccgcctccgc cagagatcga cagtactaag atgataatag
     9961 cgatcccttt accgacatta tcgaataatg cagccaagac atagacgatc atcatgaacg
    10021 ctaaggcgat caagacagca aatgacacgc tgtagaccgg attttggaca tctactccta
    10081 gtaagaaaat attccctaat gtaacgatca agctttgcgc aatcgccatt gtcaaaaatg
    10141 tcagcatacg agcaacgaat tgttctcgtt tcgtaaaatt gtttcggtct ttctttccta
    10201 aatagtattc tgtcgtagca acacttgaaa gaagaacagc gccgacccac agacatagtg
    10261 cagtataaaa cggggtgcta gctgaaccgt tatttgcgat tggataaagc gcattggttt
    10321 gtagctcaac aggttgtgta aagaagtcac tttcttttgt tgcgtctagt ttcaataatt
    10381 tgatgacttc gcctagatcg acttcttgtt cgccttttcg gatcgcgtct gctgcttttt
    10441 ggataccagc tttgatattt ggccagtcat tgttgatcaa gtccgcagca gcgttcacag
    10501 ctgtttcgac ttctggcatt ttttcattta ctgttgtcaa tgtaccggtg atttcatttt
    10561 ttagtgctgg ccaatcattt tggataaaat cagcggccaa tcctaatttt tgctgaatga
    10621 tcggtaattc attgttgtat aggtctgccc cttcattgat gccattgacg atggtatcca
    10681 tatttccatt cagcattgta ttggcatcat ggatttcttg tccaattgca ggcatttctt
    10741 tttgatattt ttctaaaatt gccactgcat tcgttacagt tgctttcgtt gagctgagaa
    10801 gcgaatcaaa gtcgatctgg tccgctttgc tcagtgcgcc ttgtgcagtc ccaatcgtat
    10861 cgatgacttg gtctagtaga gaagtgacag tggaactgat ttgacctgta ttgataccag
    10921 caatgatgcc attgatatta ttaacaacgg catcaatttg atctagcaat tcatcaatag
    10981 ccttcgtgtc ttcattttga acagctgtgt tcaaagcaga taggcggttt tgcagattgc
    11041 ttagcaaggg ttttaattga tttagctgat caataattgg ctgtaatttc gtatttccag
    11101 cacttgtttg caatttagtc aggaactcga ccagtttatc gattgcattt tgttgttgag
    11161 atattccgtc aataaagccg ttcaatacat tgctgatgtt tttcttttca tcatcagtca
    11221 gcttaccatc ttcaatagct gttcttacag tatctattaa cgaacctgtc gttgttgcca
    11281 cttgtccaat tgacttcaat gtaacatcta cactgtttgt gatgcttggt atcgcatctt
    11341 tcagctgaga ggcacctgat tttgtttggt ttgctagatc acttgcctga ttttctaaat
    11401 ttttcacatc tggcaagata tcttgtactt gttggatgat cgtcaagcct tgttttgctt
    11461 catcgattcc ttggttcatt gtctcttgga tcgaagcaaa atcgccgtcg atctctgcta
    11521 gttgcttacc ggcattttgg atctctggga ttttttcttg caaatcaagg atgatttttg
    11581 cttgatcttt caactcaggc attttgtcat tcaatgcaac gactttttca cccatctcat
    11641 ctactttagg gatgtaatcc acgaattcat ttgctttatt cagtttttcc ttgatttcag
    11701 gtagttgact ttgtaattcg aggacttgtt ttgtgtagcc gtcgatcgtg tccaaatttt
    11761 catctgttga taaaatcata tctttaactt tattgatact tactaggttc gtatcgatgt
    11821 cgtaacctat ctcattgaat actttcaata aagtcgaact ggctgttttg ataaattcat
    11881 ttgtgatttg cgattggatc gaacttgctc ctttatcggt gattttcggt gcaatcgcat
    11941 tgattttttc atttaccgta tattcgattt ttggtttctt gatatcacca ctagtaaaac
    12001 tcagcagatc ttctgagaaa tcctttggca agtaaatacc tgcgtaatac tttccggaac
    12061 ggactccgtc ttctaattgt tcttttgaat caacgaattg ccatcctaat tgtttatttt
    12121 tatgaagcga ttcgatgact tgctccccaa tggccacttc tttaccttga aactctgctg
    12181 gcttgtctgc actgtaaaca gcaattggca gctctccagt atttccatat ggatcccata
    12241 atgctttgat gttaaaccac gcatatagcg aagggatgat catgatcgcg atgatcagaa
    12301 gtgttgcaat cgggtttttg aaaatccgtt tccagtcaag caaatataat cgaagcgtat
    12361 ttttgattga ctgcatagtt ctcccccttc ttcttttttc agctgaatta ctttaccatg
    12421 tatttcatac ttttttcaat cggctttttt caaaaaagtc gaatttttgg acatatcacg
    12481 gcaattgtct cattgtaatc tcttacatat tgagaaataa ttaaggatta cagcctttac
    12541 tttatcatga aataaaaggg aaagataatt ttatgccaaa gtttttatct attatacgta
    12601 tacgcaaaaa agctaaaaca ttttttgtct ttgcccctac aaaaatgttt cagctttttt
    12661 cgttttttat tcctgtattt cttatttttc gaatgacagg atgattttcc cagcaggagg
    12721 atgtccttca actagctgat gtgcttggat gacatgctta agtgaagcag acagattcat
    12781 tgaaataaaa acatgaaagg ctcctttttg gtaggctttg attatgccag caaaggcttc
    12841 tgcatctaga tattcctttc ttggaacaaa cgattcataa aagccttcct ttttttgccg
    12901 caaatctaga tctgggaggt catttaatgc aacatacctg ccgcctggtt tcatgatctg
    12961 gatgccggtt tctcctttga tacttccttt cgtcgcatcg attacaaggt ctgcctgatc
    13021 agcaaattgc agacctggat ttgttttatc ataagcagca aatgctgaag ctcctaattt
    13081 ttttaccttt tcctcatttt tactggatgc actggttaag attcggattc ccttttcatg
    13141 gagaagctgg atcagacttg acccgaccgc acctgatgct cctagtatca ttacagtatc
    13201 tgtcggctgg atttccgtca aatggttgat tagattataa gccgtaattc ctggcgtgac
    13261 cattccggct gcttcttccc aactcatttt atctggaatt tttgctactt ttgctgatgg
    13321 caacacgact tcttctccgt aagtcccgcc gactgcatgg acagctactc gatcacctac
    13381 atgaacagtt gtgacatctg aaccaatctc tgtcacaata ccagcgccat cattacctgg
    13441 tacataaggg aatttcaatg ttcgtatctc tttcatttct cctaaacgca aggcaacatc
    13501 ataaggattc acactgaatg cttttatttc tacacgaaca tgtccagaac cgacttctct
    13561 gggatgagca tcgatttctt caaaaacatc ttgtgcttct ccgtaaccgt taattgcaaa
    13621 tcttcgcatg gtcaccatct ccctttttct ttaagtttac gtttatgcct ttattttgac
    13681 aaaaaaaatg tcttttcgtt ttggcgcatg attcaaaaca aaatagttgt cttttttcgc
    13741 gtaacgtgtt aaattattta tctaaaagcg taaagattac gatttacgct aataaaaaat
    13801 aattggagga atttgttgat ggctaaaaaa tcaaagattg ccaaagcaaa aaaacagcaa
    13861 gaaacgattg aacgctatgc tgctttacgc caggaattaa aagcgcaaaa agattacgaa
    13921 ggcttgcgta aacttccttt agatgctcgt ccagaacgat tgaaaaatcg tgatttaatc
    13981 gatggacgcc ctcgcggata catgcgtaaa ttcgggatgt cccgtataaa attccgtgaa
    14041 ttagcacata aaggtttgat tccaggagta aaaaaagcca gctggtaatc caagtctggc
    14101 tgtaatgtaa aggaggaggg cgcatggacc actccttgga tcatcaaaaa agatacgttc
    14161 gcttcttcta gcagaaatgc tggtactaga agcagacgta tcttttttta cgcacagctg
    14221 gtcctttttt atatgtaaag gctgcatggt tacttcgcta tatttattta gtaacaggag
    14281 tcagtgcttc gatacccaca ctaattttca atgctcggtc aacttctgcc ataatgggtc
    14341 gatccaaatg gcagaccttt tctttcaatc ttgatttatc gatcgttctg atctgctcca
    14401 acaggataac ggagtctctt tctattcctg ttccagtagc tttgatccca atgtgagtcg
    14461 gaagcttagg tttagccatt tttgccgtta tggccgcaac aatgatcgtc gggctaaaat
    14521 ggttacctag attattttga ataaccaaaa ctgggcgtgt tccgccttgc tccgatccca
    14581 cgactggaga aagatcagca aaataaatat ctcctctttt taccatagga tcctccttat
    14641 tcttgatatt ctcttggtat ccgctgtcct aatccgcaag ccacttcata gtggatcgta
    14701 cctaattgat ctgctatatc ctgcatagtg atttctttgt ctgcattttt gccgataagc
    14761 gtcacttttg ttccgacagg aaattcttga ggcaatcgga tcatcagttg atccatacat
    14821 acacgcccaa tcgtttcaca gtagttccct tctaccaata gagaaaaacc ctgcatcttt
    14881 cgtggccagc catctgcata accgatcggt atcgttccga tccactctgc ttcaggagtg
    14941 atatatgttt ctccatagcc gattccttca cctgctggga gttttttgac ttgaatcaat
    15001 tcagaaacca gctcaagtgc tggctgcaat ggataaactt ccggtagtgc gtgcccagag
    15061 ggattaagtc cgtacattgc tactccataa cgaatcatat tgccgattgt ttcatcatgc
    15121 cataaagccg ttgcactatt gctgacatgt acatatcgag gaagttcggg aagttttttc
    15181 agcacttcta taaagcgttc tttttgcaga ttccaatatg tatcatctgc ctgatctgct
    15241 gtagaaaaat gggtaaataa accttcccat tctaatacac gactttcttt gatgaatgca
    15301 gctgcctctt ttacttcttc aggtgtacaa aatccgatac gacccatccc agtgtctgct
    15361 tttagatgaa tcgaaagagg cgtctctagc ttacttttct ctaaaacagc agctgctgct
    15421 tgcaaccatt cccgtgtacc tgcagttact gataaatcat gagcaacagc tacagtcagt
    15481 gcgtcgtaag gaaccatatt caagataagg atgggctgag taaatccagc ttctctcaac
    15541 tcgactcctt catctaaatt tgaaacacaa aagcccgaag ctccacctgc aactgctgcc
    15601 tcagctgttt caatagctcc atgaccgtat ccgttcgctt ttactactgc aaacaattct
    15661 tttccttgcg gaagtcttgc aacttcattt gctacatttt cagttatagc ttttttatgt
    15721 atcactgctt tcgttgggcg gtgccaagcg acaaccatcg tttttccttc tttctagtct
    15781 tccaaaataa tttgtgcaaa agctgtttca tttgtatgag taatactgac ccatacattg
    15841 ccttcatgag gtgaacgcgt cacaacaggc tgaccatttt cattttttaa aatctcgaca
    15901 tcttgaaagg taacttttcc gatcccagtt ccccaagctt tagagaaagc ctctttgcaa
    15961 gcgtaacgcc cgcctaaaaa ttctacttgg cgatgaaaag gcaaggactg gaatagtttc
    16021 atttcatcag aggtcaagat tcgtgcaatg aattttggtt tttcttcgat cagccgagtg
    16081 atcctaggaa gttcgactgc atcgatccca attcctttta tcatatgctt ttctcttttc
    16141 aataaattta gaacactctc attctaccaa attataacca ttttgccaca aataagtcca
    16201 caaataacaa aaatgaaagt ttctcttaaa aaagagaaac tttcatttta tgtttaatcg
    16261 ttatttgaac ggattacaaa tccgcgtttt ttatcattgt gacgtttacc gccatctttt
    16321 tggctgttat tatttttgct ataaccgcct tttgatttgt ttccacgata attaccgccg
    16381 tcacgacgat tacggttgtt tccgccacca cgtttgccat ttttattgta tccttttttc
    16441 tgcattggca atgggcgttc tggtgtgatc ttcactggca ctgcatcagc tggatctttt
    16501 gctgtcgttt ttagcaaaag tgcaactaaa tcttgtgcag aatattcttc taataattta
    16561 tctgctgttt gcaggtattt atctagacca ttttctgcaa gttttgtttc gatttgttca
    16621 actgcagcac ctaattgacc tttgaatgct tctttttcgg ttggtggacg taaagtcgtc
    16681 atccgttttt tcgtcaagtt ttcaatcaca tgcaagtagt ccatttcatt tggtgtaacg
    16741 aaagtcacag acataccgcc ttttccggca cggcctgtac gtccgatacg gtgaacgtag
    16801 ctttctggat cttgagggat atcatagttg tacacatgag tgacacctga aatatctaat
    16861 ccacgtgcag caacgtctgt cgctacaagg atatctaaat gaccgctctt aaatgaacgc
    16921 aatacgctca ttcgtttttg ttgagacaaa tcgccatgga ttccttctgc acggtaaccg
    16981 cgtgcttcta atccgcgtgc caattcgtct acacggcgtt ttgtacgtcc gaagacgatt
    17041 gttaattcag gtgtttgaac atcaaacaag cgagtcatga tatcgaattt ttcgtattct
    17101 tttgcgcgga catagtattg atcgatcaag tcagctgtca tttctttcgc tttgatcttc
    17161 acatgatgtg gatttttcat gaatttaacg ccaatattct tgatagctgg cggcatcgtt
    17221 gctgagaaaa gtaatgtttg tctttgatca ggaacttgtg aaatgatttt ttcgatatct
    17281 tccaagaagc ccatattcaa catttcatct gcttcatcca aaactaatgt ttgaacggtt
    17341 ccaagtttca atgtgtggcg gttaatgtgg tcaagcatac gtcctggtgt acccacaacg
    17401 atatgtggac gatctttcaa gccacggatc tgacggccaa tatctgctcc gccgtaaacg
    17461 gcttgtacac gtactttttt gtcgcgccct aaacgatata attcttcttg tgtttggata
    17521 gctaattcac gtgttggtgc gataactaaa ccttgtaatt cgtgacgatc tggatcgatt
    17581 ttttctaaca ttggtaatcc aaatgcagct gttttccctg tacctgtctg tgcttgtcca
    17641 ataacgtctt ttccagctaa tgctagtgga atcgtttctg cttggatagg cgttgcttct
    17701 tcaaaaccag cgcgttcgac tgatttcaac aattctggtg ataattctag ttctttaaat
    17761 ttcaaatgaa tcctcctata tggttgttaa ctgaggatgt agcttttttg ctaccccgtc
    17821 gatttgttcg gtcaaagtgt attgtttctt tttgacagtg gccacgcacg tttttatcca
    17881 ttcttagccc ctgtggcacc accgaaaaat cactttgcga agtttaggca ttagaaaagc
    17941 caaacgggtc gcgttcagct ttatcattat tatgacatct ggcttcaaca agttgtttct
    18001 atggcaaaac cagtccaatt tctcttcatc tttcggcaat aggttattct aacatagttt
    18061 ttcgttttct gcaagtttac agatttttat ttcatttgct gaagttgttc taccacttca
    18121 aataagccca tgctgttgct gccttttagc ataatactat ctgttgcttc aagattttgt
    18181 ttgatagcat caatcaattt attcttttct ttttccaaat agaacacagg tagttggggg
    18241 tatttttggt tcaattcttc ttttagtgca tgcatttgtt ccccataaag gaaaatcatg
    18301 gcgaatttct caggatcgat atgttcactc atttgcgcat gcatatttaa agactctttc
    18361 cctaattcca gcatatctgc taacacagcg attctgcgtc cttttgttgg cagattttta
    18421 aaagtatcta ataccagtcc catggctgtt ggatttgcat tgtagacatc gctcaaaata
    18481 gctgcaccat ttcctgccgt tagccattcg gttctgtttt gagtgataga aacatgggcg
    18541 agcccttggc gtatctcagg tatggacagt ccaaagaagc gcccgatcgt atacgcaatc
    18601 aaagcattcg ttacattata acttccaggc agcgggatcg taaatgtttc tccttctact
    18661 acaaaatcgg tttgtgcttt tccttttgct gtcacttccg catgaacatc gccatttcca
    18721 ataccgaatg ttgtgactgt ttgtactaat gttgccgtta aaggtgtcag caacggttca
    18781 tctgcgggca caacgagtaa tccgtctgat acaagtccgt cagtgatttc cattttggct
    18841 ttagcgattc cttctcgcga cccaagattt tctatgtgtg cttcaccgat cattgtgatt
    18901 gctgcagctt ttggttgagc cataagtgaa agttccgtga tttcattggc gtgatccatg
    18961 cccatttcca aaacgatctt ttctgtatct tggggcatgt gcaaaatcgt gtaaggaaga
    19021 ccaatattat tgttgtaatt tccttgcgtc ttatatgttt tgtatttttc agacaagaca
    19081 gacgcggtta gatctttcgt ggtggtttta ccattgcttc ctgtaattgc aacgacttct
    19141 gcgcctactt tttccaagta ataggttgcc aattgctgca tggctttcaa aggatctgct
    19201 acaggaataa ctggaaaatt ttttggagct tgttcaacag gcaagctcca aaatgaagca
    19261 gatgctccgt ttgtgatcgc ttgctggata aattcatgtc catctcgcgt accagcaaga
    19321 ggaacgaata aacttccttc ggttatcttc cggctgtcaa attcgacatt ggtaatcttt
    19381 gtcagtgcag agcattcgct gcccacggct tgtgcaattt cttgtattgt tagattcatt
    19441 gagatctctc ctaaaaaagg ttgtgaaaaa agcgtttggc ctaaacggcg ggttcgcatg
    19501 actctcactt ccaagtaaat ttctctattc gtatttagaa gctagaatga acgcggttac
    19561 gcaatcactc cagcctcttc tttatttctc tttattcatt tttttcaaaa aaagattgac
    19621 gctgatggta tcgattcatt cctaactgga tcagttcttc aatcaaatca ccgtatttca
    19681 agcccatatt ttcccataag agtgggtaca tactgaactc cgtaaatcct ggcatagagt
    19741 ttaattcatt caggaataat tcatttttat ttgtcaaaaa gaaatcgcac cggctcaatc
    19801 cgcttccacc taacatcgtg taagctaact tcgcgtactc ttgcgctttt tgataaactt
    19861 cttctggcac ttcggctgga atctgcattt cgattttatt attgatatat tttgcttcat
    19921 aatcatagaa tgctacgtct tttacgactt caccaggcaa agtcgtccga acgtcttcat
    19981 ttcctaatac agcaacttcg atttcgcgcg cttcaattcc ttgttcaacg attgctcgag
    20041 aatcatactg ataggctgtt gctaaagcat tttgcagctc ttctcggttt tctgcttttg
    20101 taatgccgac actagaaccc atattcgccg gtttgacaaa catcggataa agcaaagaac
    20161 cttcacattg atcaaatact tttttaggat tttctttcca ttgattctta agtactggta
    20221 cataaggaac ttgcggcaca ccagcagctt gtaaaatata cttggtcatg attttatcca
    20281 tggcacatgc actggtcaat acgcctgcgc cgacataagg catattcaat gtctctaaga
    20341 agccttggat cgttccatct tccccgtttg gcccatgtaa aactggaaaa acgatggctc
    20401 cttcttcttt gatttcgccc ggattgatca cttttcctgt aaagccttcc tctgtctgtc
    20461 cacttgggtc ccatgaaaga tgcaagacat ctttgctggc aggtttttct gttagtaatg
    20521 gacctttgac ccattgtcct tcttttgtaa taaatacgag ttgaacttgg taataattat
    20581 aataaatggc atttaaaact gaaaatgcgg aaagaatgga cacttcatgc tctgcgctgc
    20641 gtccgccata tagtaaagta atcttcaaaa gattgtcctc ctgtattcta aatcgttcag
    20701 tgatttactc tcatatttta gcacaaaaca aaaataaacg aataacttat ttataaaaaa
    20761 acgagccagc ttctaaaaga acgagcgtca tttatttttt gtcagctaga ttatttgtcc
    20821 cgttttttat aaaaagttat gataaagaaa aggagggatg atgatggcgc aaacaaatcc
    20881 acgcagccgc ttggcagaaa tcattcgagt gttcatcaat tacaatgtag tgccaaactt
    20941 tgtacagcaa aaaaatcccg aacaggtaaa aaaagccttt gaagaactag gccccacctt
    21001 catcaagata ggacagatgc tttctgttcg agaagatttg ctttcttctg ccttcactca
    21061 aacattcaaa acattacaag atagtgtacc tagcgacaca ttttcaacgg tcaaaaagac
    21121 gatcgaaaca gaactttctc tttcattatc agatatattc gatgattttt caaaatcgcc
    21181 ttttgcctct gcttctatgg ggcaggctca tcgtgccaag ctgaagaacg gtgacgcggt
    21241 agtcgttaag atccaacacc ctaacatcgc cgaagagatc cgacttgatc ttcaactatt
    21301 tgaacgagcg atccctttga tcaaatacat tcctgaaact agcgtagttg atctaaaagg
    21361 ggtactccaa gaagtaaaac gttccttgat caatgaaatg gacttcttaa aagaaagtca
    21421 aaatggcgaa caattctatc aaaagaacaa tggctggaaa gagatccgca gccccaagat
    21481 ttatgacgct ttttgttcga aaaaagtgat cgtgatggaa gaaatgtctg gtaaaaactt
    21541 gaatcattta atgaacgccg aaaacaaaac tgaaactttt atcactggaa ttcaaaacaa
    21601 gcagctgaaa caagaagttg caaagctttt agttgagaac tttatgaaac aggtgtttga
    21661 tgatggtttt ttccatgctg atcctcatcc agggaatctg ctattccatg tactaacaaa
    21721 agaagaacag acacaagcta gccgaaaaac tgaaacagtc catgaaaaag agttcggttc
    21781 ctttgctttt cgagcctcca cttctgcaga agaccctgtt gcgccatata ccatcaacta
    21841 catcgatttt ggtatgatgg ggcatctttc tgcaggactt aggcaaaaac tcacacaagc
    21901 cgttttagcg ctgtatacga aagatgctta tcgaatcgaa aaagcggtcc ttcgactttg
    21961 tcagcaagaa ggctcgtttg atgaaagtcg gttccaccaa gaactgacaa gttttcttga
    22021 acagtattat gattctccta ttgatgaaat caacttgcaa gaagtattca cacatgtcgt
    22081 gacgatttgt catcaaaata atctgcagtt cgatcgtgat atcacgcttt tgctgaaagc
    22141 atttgggaca ttagaagggg tgatccgcgt cctcgatcct gaagtttccc tcatggaagt
    22201 cgcttcgcct tttgctcaac attattttct cactcatctg gatgtagaag atacattgaa
    22261 acaaagcgga ttagacttat tggaagggat gaaggctgcg cctaagatcc ctcaacagct
    22321 ccaccatcta ttagaaatgt ggacttcggg acaaggaaaa gtcaatttgg aattgaaaaa
    22381 gcaagacaaa ttattgagca ggatcgaaag tatgatcaat cggcttgttt tcggtatgat
    22441 tttagctgca ttgatcgtag ggtcctccct gcttgtccaa gcagcacctg tagaaaacgc
    22501 agaggtcgtt tccttattag ggatatttac gtatgctatt gccgcattcg tcattatttt
    22561 tttggcaatc gatgcattga tccaaatgta taagaagagg aaaaagtaga aaaaggttgt
    22621 gacagaaaga atcagcttca agaaataaga aggggtttta cataaattgt ttctcaaatt
    22681 tttgtgaaaa ttcggcttat ttttcgaaga ggctacttct gaaacaccga gaataaaaaa
    22741 tgtgaaaaag ctgttctgca cccagtaata agaacaatca aaaagggaac atggaacaca
    22801 tttatcctgt ttccctgttc cctttttgat gtattcgagc ataaataaaa taccttcgtt
    22861 acctactatc ttctgtactt ggtaaacctt agtcaattgc cttttttaac gcttgacgaa
    22921 aagacatatg atctttctcc gtaaagggtt ttggaccctt tgtccttatg ccagcttttc
    22981 ttaactttcc actatgataa cgttgttcta gaagcgtgcc gatattcgct tcgtcaaatt
    23041 cttttccaga gtgatggaac actcgcacac ccttcggcaa tacaagtgaa gctaaaccat
    23101 aatcttgcgt gatcaaaaag tcattcttct taaccatacc gacgatttta taatccgcac
    23161 tatcagctcc gcggtctacg tagacgattt ggacatgatt agcgtattct ttggtcgtat
    23221 aatggtccac actggtcacg ataaccacgt caagaccata ggattctcct aattttatga
    23281 catcttcttt tactggagaa ccatctccat caatgaatag cctcattctt tttcgccttc
    23341 ttccataaat gataaacagt tactaatccg ctcagtaaaa gaccaacatt gatccatttt
    23401 gccaagataa gcagttgtac actttctatc ggcactgatt catggttcag cgtgatcaat
    23461 aatagatgga ttggctctcc catttctttt gctaaaacaa acaaaagcgt catcaataaa
    23521 aactggtgga tcacactccc tttattttga gtagtatact tgacaacgat ccggcgatcc
    23581 attggaatag ctgtatcagt ttgataatat tccttattta gccagctgcc tgttactacg
    23641 tgccagaaac taagcagaaa gacacctatc gcaaaaccat ttggaatcaa gaggaccaat
    23701 aaagaagaac tcagtccaag caattgcata gccaatggag accatgttaa acgtttcaaa
    23761 atccatggtc cgactatttt tgctgcaatc aagcctgctg cataaggcaa atacataaaa
    23821 atcgaaagtt tttctaatcc gtacagtatg ccaacataaa aactgctaaa taaaaaaagg
    23881 aaattcccta gcattccatt tagaaaagta aacagattta gccagcttgg caacttccat
    23941 tccttcttct ggtgtcctaa aacaaacacg aacaagatga acagcagcaa gaaaccataa
    24001 atagcaagat caaaaagcct ttcatcgaat aacaatcggg catttctcaa aagaaacaat
    24061 aagccaaaga atatcaaaaa taaaacaagc tctcgcgtag ctaccagatt tcttttgatg
    24121 tctttgaaat ccagttcgta acgcggataa tgactaaccg tatggtaggc catgacgtaa
    24181 aaaagtgtat aaatagtaaa gcttgctgca atctgagtcg gtgcaggcaa ctcaatcgct
    24241 tgatacagga gaaatagcca gatagctgca aataagtact ttttcccatc catattgata
    24301 aaaccttgcg tcttttcgtg aaaattgacc gtggtattag caggtacaag ccatgctgca
    24361 ctcagcccta gcaaaacact tgaaaaataa aataaaggaa aataaaatac gcctaatatt
    24421 ccaagcaatg aaccagcacc accaatcagc agagcgatca tcagcaacgt gtaactatct
    24481 agaccaaatc gtagactgtt gactaaaaat agtccagtca tccgaaaggc ataaaacagc
    24541 gtaaatggca aaatcgtcag ccaattatgc gagtctgtta gtcccaaaaa taaaagataa
    24601 ggaataaaaa taaccgtgtt acttaagata aagggcgttg tttgtgtaaa attacgccca
    24661 aatcgtttga gtttttcgaa catgacaatg gctccttaaa cagtcttata actaatctgc
    24721 tcttcgattt tagcattaaa ggggaaataa accaatttta cagaatatat tcttgtaaaa
    24781 aaaaatacag aaagaagcat aagcttcttt ctgcattttt acggtacttg attactcaag
    24841 cagacaagcc cttctccaca aaagtcttgc ccgacacccc tgcatccggc aggtgataaa
    24901 gatagaaatc aattatcctg tgcctccaaa atcaattagg agctctgtaa accagagtcc
    24961 ttctcgaccc gtcgcataaa tttactttaa cacagttcct atgtttatgc gagagctttt
    25021 ttcattttat aatcgaccat ttgaaatcct tgaccgatat ctgaaataag ttcttctgta
    25081 atctcaaatc cttcatgctt ataaacctcg attgcttgat gattcccctg attcactcgc
    25141 aaaaagatct ctctttcctt tgtttgcgca gcaagttctt cataccagcg aaaaactgaa
    25201 gaagtcaatc cttgaccacg tactttattt agcaaatata atttacttaa ataaattttt
    25261 ccatttatct cttcataagc agtgtaaccg acagtctttt cttcgctttt taataaaaag
    25321 taggacacac cttctgtcag ttctttttga atctgttttt ttgactgata agttttcagc
    25381 atgtaatcca cttgtttttg accaatgatc ggcacaaaag cttccggcca tacttcttgt
    25441 atgactgcca tcagctcatc cagttcattt tctgttactt ttattttttc catacaccca
    25501 cgtccttttt tacattataa atgaccttgc gcttgatgga ataaaaagag catgatcatg
    25561 ctatacacac ccaccgacca tggtttcaag aaaatcacaa tctgcataaa ttccctaaaa
    25621 gataatttcg tcaaaccagc tactagacag atcaagtcgt ctggcgcaaa gggaagcagc
    25681 aaggtaacaa ctagtacttt tttaatatct ttcgtttttc ttgtcagcaa ttcatcaaat
    25741 ttctgatact tttttggcga taaaataagc tggacaaagg ctacgccata gtaacggaca
    25801 agcaaaaatc cgattgcttc cccaatcact aggccaatac aactatataa aagcccaaat
    25861 ccattgccaa acatcagcat gcccgctact gaggagatcc ctcccggcaa aataggaatg
    25921 atcacttgga tcacttgcaa cagaacaaat atcatcactg catacttgcc aaattgctgg
    25981 ataaacgctt gcaaagacgt gctcgatcga aaaatcccat gatgataagc ataaagaatc
    26041 aacacaaaga aaaaaaacag accaataatg ggcatgatct gaagacattt ttgtaaaact
    26101 gatccttttt tagacatcta tattccccac atcttttttc atttattaaa actgcctcct
//
LOCUS       DACJGB010000036        25536 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.036, whole genome shotgun sequence.
ACCESSION   DACJGB010000036 DACJGB010000000
VERSION     DACJGB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.036"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IYI34_001560"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IYI34_001560"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAR0559431.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IYI34_001561"
     CDS             complement(937..1518)
                     /locus_tag="IYI34_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0559432.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IYI34_001562"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IYI34_001562"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAR0559433.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IYI34_001563"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IYI34_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAR0559434.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IYI34_001564"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IYI34_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAR0559435.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IYI34_001565"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IYI34_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAR0559436.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IYI34_001566"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IYI34_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAR0559437.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IYI34_001567"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IYI34_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAR0559438.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IYI34_001568"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IYI34_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAR0559439.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IYI34_001569"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IYI34_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAR0559440.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IYI34_001570"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IYI34_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAR0559441.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IYI34_001571"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IYI34_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAR0559442.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IYI34_001572"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IYI34_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAR0559443.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IYI34_001573"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IYI34_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAR0559444.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IYI34_001574"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IYI34_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAR0559445.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IYI34_001575"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IYI34_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAR0559446.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IYI34_001576"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IYI34_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAR0559447.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IYI34_001577"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IYI34_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAR0559448.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IYI34_001578"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IYI34_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAR0559449.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IYI34_001579"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IYI34_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAR0559450.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IYI34_001580"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IYI34_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAR0559451.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IYI34_001581"
     CDS             13972..14157
                     /locus_tag="IYI34_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAR0559452.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IYI34_001582"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IYI34_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAR0559453.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IYI34_001583"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IYI34_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAR0559454.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IYI34_001584"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IYI34_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAR0559455.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IYI34_001585"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IYI34_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAR0559456.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IYI34_001586"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IYI34_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAR0559457.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IYI34_001587"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IYI34_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAR0559458.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IYI34_001588"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IYI34_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAR0559459.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IYI34_001589"
     CDS             18232..18879
                     /locus_tag="IYI34_001589"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAR0559460.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IYI34_001590"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IYI34_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAR0559461.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IYI34_001591"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IYI34_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAR0559462.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IYI34_001592"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IYI34_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAR0559463.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IYI34_001593"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IYI34_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAR0559464.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IYI34_001594"
     CDS             20317..21255
                     /locus_tag="IYI34_001594"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAR0559465.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IYI34_001595"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IYI34_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAR0559466.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IYI34_001596"
     CDS             21935..22774
                     /locus_tag="IYI34_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR0559467.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IYI34_001597"
     CDS             22750..23619
                     /locus_tag="IYI34_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR0559468.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IYI34_001598"
     CDS             23609..24406
                     /locus_tag="IYI34_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAR0559469.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IYI34_001599"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IYI34_001599"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAR0559470.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4712 c         5497 g         7028 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctc
//
LOCUS       DACJGB010000037        25516 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.037, whole genome shotgun sequence.
ACCESSION   DACJGB010000037 DACJGB010000000
VERSION     DACJGB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.037"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IYI34_001600"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IYI34_001600"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAR0559471.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IYI34_001601"
     CDS             complement(860..1678)
                     /locus_tag="IYI34_001601"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAR0559472.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IYI34_001602"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IYI34_001602"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAR0559473.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IYI34_001603"
     CDS             complement(2967..3923)
                     /locus_tag="IYI34_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559474.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IYI34_001604"
     CDS             complement(3923..5056)
                     /locus_tag="IYI34_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559475.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IYI34_001605"
     CDS             complement(5049..6611)
                     /locus_tag="IYI34_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559476.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IYI34_001606"
     CDS             complement(6823..7905)
                     /locus_tag="IYI34_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0559477.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IYI34_001607"
     CDS             complement(7971..8369)
                     /locus_tag="IYI34_001607"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAR0559478.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IYI34_001608"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IYI34_001608"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAR0559479.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IYI34_001609"
     CDS             complement(9098..10399)
                     /locus_tag="IYI34_001609"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAR0559480.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IYI34_001610"
     CDS             complement(10540..11556)
                     /locus_tag="IYI34_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0559481.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IYI34_001611"
     CDS             complement(11864..12469)
                     /locus_tag="IYI34_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0559482.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IYI34_001612"
     CDS             12665..13588
                     /locus_tag="IYI34_001612"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAR0559483.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IYI34_001613"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IYI34_001613"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAR0559484.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15703..17334)
                     /locus_tag="IYI34_001614"
     CDS             complement(15703..17334)
                     /locus_tag="IYI34_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAR0559485.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17348..19021)
                     /locus_tag="IYI34_001615"
     CDS             complement(17348..19021)
                     /locus_tag="IYI34_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0559486.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19034..19351)
                     /locus_tag="IYI34_001616"
     CDS             complement(19034..19351)
                     /locus_tag="IYI34_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAR0559487.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IYI34_001617"
     CDS             complement(19376..20776)
                     /gene="lacG"
                     /locus_tag="IYI34_001617"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAR0559488.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21013..21798
                     /locus_tag="IYI34_001618"
     CDS             21013..21798
                     /locus_tag="IYI34_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR0559489.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21801..22493
                     /locus_tag="IYI34_001619"
     CDS             21801..22493
                     /locus_tag="IYI34_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR0559490.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22767..23138)
                     /locus_tag="IYI34_001620"
     CDS             complement(22767..23138)
                     /locus_tag="IYI34_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAR0559491.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(23538..23768)
                     /locus_tag="IYI34_001621"
     CDS             complement(23538..23768)
                     /locus_tag="IYI34_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559492.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(23862..24200)
                     /locus_tag="IYI34_001622"
     CDS             complement(23862..24200)
                     /locus_tag="IYI34_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559493.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(25128..25244)
                     /locus_tag="IYI34_001623"
     CDS             complement(25128..25244)
                     /locus_tag="IYI34_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAR0559494.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT         7436 a         5603 c         4499 g         7978 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aacgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcactg attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggcccctagt ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagc agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatcca ttaggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcaa tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat aaagagccaa cataatatgg agggggaatc
    15241 cctataaatc aacattcttc aatagctaaa atgcttgatt ttcattaaaa ataagatggt
    15301 tccctaccaa aagagactat aatataagtt aatttgtttc attcttatca taaaaagaag
    15361 ttctcgaaaa acgaactgtt ttccgagaac ttttttctat ccatctaagt gctcaaatca
    15421 tattcacgac ggaattctaa ggttaccctt tcttaaacaa actttatgtt atctgcacaa
    15481 ctttttgcaa ttttttcatt gcttcttacg aaatcgttga actctcttta gcataataca
    15541 aagtgtagat gaagtctggt gctttactcg ttttggtaaa aatgactgga aatcgtttct
    15601 ggttattttt ggcaaaggcg aggtgcaggt tcgattcctg ccttctacgc taacgatgtg
    15661 gctgaaggga acctccgatc ttgaatcgaa acgatccgca gtctattgtt tcaccaatga
    15721 aacaaaatca acgttcgtcc aagcatttcc tgaagccttg ctataaaccc caatttcaca
    15781 aacacctgtt gtaattggaa tgtctttgat tgtaattttt gtccattcac ttgttttaac
    15841 aaccgcagta tttagttccg ctccaccata atttttgata tataactgat tagtatcaaa
    15901 ctctccccca gacattacat aggcactcaa tgtgtaagtt cctttgctta tgccactaac
    15961 attttgataa gctgatgcct cgtatgcttg atcctgccaa aaagttagtt tgtagtcccc
    16021 aaaaaaagtg cccgcttccg ttttgatcgt acccttagcg gttccttgtt gaaaccaggt
    16081 ttgccaattg gaaggactgg tcgttggtcc atcgctttcg aaacttgcat ttttaactag
    16141 atttatttgc gcccctgctt gtttaaacgc acgcaagctt ggcaaggcgt ttccagcaaa
    16201 atcaaacatc gcttggttgt cccattcgtt tcccatagtg gattgaacac tgagataatt
    16261 catacctgct tgtgttgccc aactcacatt tccgttgtac caaagaggtt cccaatagaa
    16321 gaagcccgcc ccgcgattat tcggtactgc ctgaatccga tcggcaagat ccagcaaata
    16381 ctctgcttgt cctgtcggtg ttggttcata tccccccgca gctgcttcgg cttcaccaaa
    16441 agcatttggc atagtatcgg cattcgctgt tgtatgccca taagctgttt caactacgat
    16501 cacttcttta ccataccgtt ggctgatcgc attcatattg gtttgaagtt cattcattga
    16561 accgtgccaa tacggataat acgacactcc aattaaatca aatgacacac ctcgactcgt
    16621 gatttcatca aaccaccaag taaatgctcc tgtatcgcca ccatcagcta agtggagcat
    16681 tactggagtt gttttaggtt gagtgtctcg aacagcttgg ataccagact tcaaaaaagc
    16741 agccaatcga tcaaattctc ccccgccttc tccccaagac ttgccataag gccagagcat
    16801 tccgctattc agttcattgc cgatctgaac catatcagga taaacgtcca gctgcttcat
    16861 ttccgacaga acgttctttg tatagtcgta caatgccgta ttcatttctt caaaggtcag
    16921 accttgccat gctttaggaa gattctgttt cccaggatca acccaaaaat cactgtagtg
    16981 aaaatccagc agcaccttaa gtcccagatt ttttgctcgt tttgcaaggt caatcgtcgt
    17041 ttccagatca tttgtccctg ctccataact ttctccatta ctattataag gatcctgcca
    17101 caaacgcagt cgaacatagt tgacaccatt attttttaaa atctctaagg catcttttga
    17161 aatatcgtct tcataatatt ttgcaccaga tttttccata tctgacagga tcgaaatatc
    17221 tgctccgcga ataaattcgc tggcagatgc ttcaacagtt tttgaaacag cccccaaagc
    17281 ccctaacaca gctgcaaata aaacagcttt acgcaaccaa attcccattt tcattatggc
    17341 tcctccttta ttgaaattgg ttcagaataa actgaacagc tttttcagga tctctcgtta
    17401 atccgatata ttctgctcct tttgtcgcaa ccagcttgat tcctaagcga tcggtgtctt
    17461 ctttgatatc atcatagtaa gaattgactt gcggtgctaa gaccaccata tcaaaatcct
    17521 gcagcatttc gtaatgagaa ccataagcac ccgccattgc actgatcggt gctttcgatt
    17581 cagatgcacc tttttttaaa gcattagcta acatcgcact tgtccctgcg ccagcacaaa
    17641 gtaccaacac attgatttct ttatttgcat cgctgataac ttgactcgct gccacaggta
    17701 cttcctcacg tacatcggca gaagcacctt cctcggttgc gatcacctct gctttttgtg
    17761 cttctttctc tactaagaca gcatcgtatg ctttgcaaaa tggtagatag atcaggaagt
    17821 caacgacgat tagaaccgct gctagaacaa aagctaagat acccatgccc gttcctagaa
    17881 ccaaaccgat cggtgctgga gttgcccaag gcaaaacgta cataaagctg ttcatgccca
    17941 atacatcgac aaaaaacttg aacaggcaaa cgtttacaac aggtgttaac aaaaatggaa
    18001 taaaaaaata agggttcaaa atcatgggtg ccgcaaatag caatggttcg ttgacagcga
    18061 aacttactgg aataaaggaa gctttcccaa ctgccttcaa ctgcttagac ttagcaaaga
    18121 gtaggaagat aaaaggaaca accaatgttg ccccagttcc tcccatagtt cctacaaaat
    18181 tccctaaacc caccgtcagt actttagaag cttgttcacc ggaattgaac aattgcagat
    18241 tggtttccac attggcatag ataattgccg caattgccgg ctccacgatc gacggtccat
    18301 gaacaccgac aaaccaaaac agcgccatcg ctccccagat aatagcgatt cctaagtacc
    18361 cgtcagctgc tgtaaatagc ggctgcagca ccgtaatgat tgcttctgca aatgagattt
    18421 ggaagatttg tcgaatgcct aaatcaatta aaacggccgc aaatactgaa aaagagaaag
    18481 gaaaaatatc tcggaaggtt tgcgaaatcg ttccaggaac ttctttaggc attttaatgg
    18541 taatatcttt cagcacacaa aacttatata aattaactgt taaaaatgca gcgacaaagg
    18601 aagccagtag tccctttgtc cccatatatt ccgttgaaaa tccaccttca atcggagaga
    18661 cgcttaacaa caggaaactt accacagagg ccagcatcac agaggtggtg ttgattactt
    18721 tgtttttcgg taagcgacga ttcatcgaat cagccaagca gcgagcagtg gttgctgaaa
    18781 cgagtaaccc tacaacaccc atcgaataat tatagacttt ccatagccat gtggagacag
    18841 attcaggcca ttggtaacca aaaatctcag gaaccgcagc aatcaaaatg aaaatgcttg
    18901 aaaataaaat agctggcatc gctgttaaaa atccgtcacg aacggctcct aaataactat
    18961 tacgtgagat tttttcaaaa aatggtttcc ctttttcgat ttgtttaatg attccatcca
    19021 tagcgttctt ctcctatcct tgattctgtc tgtacagtac aataaagtct tgaatcagat
    19081 ctctcagcaa taaagtggtc attaaatgat cctgcccatg gataaaaatg aatcccatct
    19141 ccatctcttt cccagatgct tcttccgcta aaatttttgt ttgcgaatta tgtgcaaggg
    19201 ttaaattttc gtcggcttct ttcaacgccg aatcaacatg ttcaaagttt ccttgtctta
    19261 cttctttcaa taatttcaat aatgtactgc gagcgtcgcc tgaatatgca acgatctcaa
    19321 aaccaagcat ttgtaattcc tctttattca tcttttcttc ctccattatc acttattaaa
    19381 taatcgtttt ggtttcactg accaacttgt accagtaagc tgattctttt gggtaacgct
    19441 tctgggtttc aaaatcaaca taaaacaagc catacctttt gttataccca tttgtccatg
    19501 agaaaagatc cataagcgac cacaaaaaat accccttcac atttaccccg gctgttatcg
    19561 ccttgcttaa ggattccaaa tagactctca aatagtcgat tctcggctga tccataataa
    19621 tgccatcttc aaactgatct ttatacccca tcccgttctc agtgatatag attttgttgt
    19681 aatgtgggta atcgctttta attcgcagca acaagtcata tagaccttct ggataaatca
    19741 gccagtccca atcagttcga ggaattcctt ctttataaat acgttctccg attcccttca
    19801 ctttataaac agaggtccct ttttcacccg taccgttatg atggattgca ttttctccat
    19861 cgtaagcttt gacaaaatgg cattggtagt gattgatccc taagtagtca ttacgcgtgg
    19921 atgctttttt caattcaacg aaatcttctt caggaaaatg atagctggcc tgatttgctt
    19981 cacatatctc atcaagagca gtcaaggttt cagttgaata atagcctaaa taggtagcat
    20041 ctaataagaa acggatcgac aatgcatcgt ctaaaaaggc agcatgctta tcttccggcg
    20101 cgtctgttgc cgcatatttc gtctctaatg agtgaactac accaatttca cccggaagtt
    20161 cgttttcttt gaaatagttc actacacggg catgagcaac catcatatta tgaagacagg
    20221 cgacgatctt tgtaaaatca tatttgattc ctggtgggaa aacacctaac aaatattgat
    20281 tggttgcaac tggatagatt tcattaaagg tactccacac cttgacttct ttgaattcat
    20341 gaaaacaaaa aatggcatag gaaacaaatg cttctattgt ttctcgattc aaaaaatctc
    20401 catggtcaaa caaacgttta ggggtatcaa aatgatgcaa cgtcacaaac ggcgttacgt
    20461 ttcttttctt acattcttcg aacacacgat gataaaaagc gattccttct ggatttggtt
    20521 tcccagctcc atcaggaaaa atgcggctcc aagcaatgga taatcttaga ccatttacac
    20581 caaaacgttc acacaattca atgtcctttg catattgatg ataaaaatca cttgccggat
    20641 cgggactgaa tcgcccttgt tcctctaaaa agtcatccca agcaacagcg cctttgccac
    20701 cttccttagt ggctccttca acctgatagg ctgctgtggc tccgccaaaa ataaaatctt
    20761 ctggtaattt aagcatgtac atctctcctc aaatctataa tcagttttcg ctctttctaa
    20821 ctgattgcat tgtaaacgca tccaaaacat ttgtcaacaa aaacaaacac aaataatcat
    20881 aaaaaccaga aaacagcaat aaaatctatt gtaacaaaca taaaaaccac cctaaatgtt
    20941 tacttttgtt cgaattagat tgactgttgt ccgtttatat gatagattat ccttaaatta
    21001 gaggtgaaga caatgttgaa aaaagaaaga ttgcttacta tcgtagaaat ggtcaataaa
    21061 aaaggaatcc ttaccgttaa tgaaatcatc aatcaattag atgtttctga tatgaccgtt
    21121 cgcagagatt tagatgaatt agaaaaatcg ggcaagctgc tgcgtgtcca tggaggggct
    21181 caaagtcttt cctacacttt agatcaagaa ctttctcaca ctgaaaaagc cactctgcag
    21241 attcaagaaa aaaaagaaat tgctgccaca gcagcctcct tgattcacga gggagaaacg
    21301 attttcttag gaccaggtac aacaatcgaa ctattggcga cgcatttact gaacaagaaa
    21361 attcggatca ttacaaataa ttatccagtt tttgatattc ttgctcaagc agatggacca
    21421 gaaattctac tgattggcgg tgatttccgg aaaaataccg gcgcatttgt cggaccgatc
    21481 accaacgaca atttaagaag actaaagttt acaaaagcat ttatcagttc aaatggcgtt
    21541 cacaacgaag cgatctctac ttacagtacc gaagaaggag aagcacagca gatcgctcta
    21601 aataattccc gtaagaaata tctactcgtt gataataaaa aattcaatcg agatgatttt
    21661 tatgtgtttt ataatttgca tgatttcgat ctgctcatta cggatcgcca gatctcgcgg
    21721 gaggttcaag agcactatgc acaatatgtc gatctgcaaa ttgctgattc aaccgaaaat
    21781 accactatag gagcgtaact atgaaaggga ttatatttga tttcaacgga acaatgtttt
    21841 tggactctca tctgcatgaa gctgcttggc tgcatatgat acatgatcat acaaaagatg
    21901 gtttatcaga cgaggatatt ttaaaaaata ttcatggtcg gacgaatacg gagattttga
    21961 aacatttcat ttcagagcat ttgaccgcct ctgagatcgc cgtattgtca gaagaaaaag
    22021 aaagctatta tcgtatgctt tgcttgcaaa atgaaaacga gcttcagttg acaaaagggc
    22081 ttgaagcgac tcttgaccag ctgactgacc aacagattcc attaacgatt gccacggcta
    22141 ctgtcaaaga gaatgttgct ttttattttg atattttcga tttagcccgc tggtttgact
    22201 gggacaagat tgtctatgac gacggctctt tccctgggaa accgcaaccg gatatttttc
    22261 ttaaagcggc agacaaactg agcttagctc cggctgattg tcttgttatc gaagatgctt
    22321 actcaggatt acttgctgcc aaacgtgcgg ggatcggaac aattattgca attgatccat
    22381 tcggtaaaaa caaaacagta tttgaacagg cacagctaaa taaagatgga accatcaaag
    22441 attttactgg tttttggaca gagcacctgt ctgccacatc cgttaattct taaacgattc
    22501 aactaactac caaagaaaag caactgaaat gcacacctcc ttttataaaa aactgccagt
    22561 gaaacgacat gaaagataca ctgctatatc cttagtgaaa tatagcagtg tatctttgac
    22621 tttttcattc ttttaaccat atgacgcgtt catctctatc tataattaac attaaaaata
    22681 gtgttttcac attatttctc aaaccaatct taaaaatcat caggagtact tttttgatat
    22741 acttttatta atgctgggcg taatggttaa aagatttcca ctagttcctc tccaccaagc
    22801 tgagttgaaa aatgcttatc tgtaaacaac atagacaaag aggaaataaa cgtttccgta
    22861 tctttctctg tcccaaggat attaattaat tttcctacta ggtaagtatc attaaactta
    22921 agatctgcct tcacatcaaa agttttgata aaagctttta tcatgcgtac aattttgtct
    22981 ttatcgccat aattgatttc accactcata tcaatatagt cactaatccc tgaagaaata
    23041 atggcaactg cttcttcttt cgccttatca gataattcat ttaatgactg ggattgattg
    23101 atgacttctt gagttttatc tgttacgtaa tctctcatta tcctatcggc atactccgca
    23161 aagtaatctt gaaaaaattc ttctacaggg taaccaactt cgttttccca accaggagaa
    23221 atttcattat cagtacttat taaatctatt aaaaaccaac ccgttctctg agtgttgatt
    23281 ctataatatt tttaatttcc tatataaatc aaaaatatta tagaatcaat aagctaatta
    23341 agaaacaaac tttagaaact ctagaagaac ttctgaattt atatagaagt gtgtagctac
    23401 ttactaaata tacaaaagtt aaaataaaat actctggata ctctttatac tgcattaaag
    23461 ttagtaatat taacagcaaa gatatcgaaa aaacaataac ataatatgtg tcaaaatttt
    23521 tcataataag aactccctta ctgttgcata ctaacataat ccaaaaactc ttctacttca
    23581 gctaaagaca ttccactttc tttagtccaa aaaacagttt tttctatcga tccttctctg
    23641 taaactgtaa atgtcggagt cgagttaatt gagtagcgac tcttgaattc tttccactca
    23701 ttttcatctt ctctaacttt tgctacattc acgtaataaa tattttcttt tcccttttct
    23761 accagcatat cgaatagctt cctgcttttg atgatcgatt tcttgtgcta catctagtaa
    23821 attttcatca atttctatat cataaagtat ttcactagtg tctacatcca caatatatac
    23881 tttttatcag tagcaatttt tgagtaatcc gttaaaatga tctcagtctc gtcactttca
    23941 tcagtataaa tatacgttaa caatgaactc accttttcaa tcggtgttag ctctaagcct
    24001 actgtatttt ttactattga gtcattattt accaccataa cttcgtactg tatataccat
    24061 cctatttggt ttccatcaat atcagtaatt ggctcactag ctaatggagt ctccggaaca
    24121 atattttcct gatatttctt aaactcttct gtagcacgaa aaaggttatc atgttcctca
    24181 cttgaaactg gttgagtcat agaaaatgtt acttggctaa acagagggaa aataaacact
    24241 acaatcaaca aagtataaaa aaaagccctt ctttttcatt ttttatcttc caatcacaaa
    24301 ttgaaaaact tattcctgca actttgagtc tagtatcaca atcgactgac tacaactatt
    24361 catttacatt ctactttaag ttcctcctta accattaatt ttattaaaga tacttgtaat
    24421 agtgattctc acaaaatttc atgaaaaata ctttattact gtatctaaaa ttccaataca
    24481 caacttatcg actattatct gaaaaccaaa acttattatt ccttgatgtt attctagcaa
    24541 tagtaactgc ttttttattt ttttgcatat tttttcggcg ttacttttta aagttaactt
    24601 tttcgacaaa tagaaagaga tacgaatcac tgattttttt gaagaaaaga aatggagtga
    24661 gctgaacgtt agagatattg ggagctttcg taactgctta ttaaacgata atggttattt
    24721 gcaaccgtat tgctcgcttc tacatgaaat atttcatgaa atatttcgca aaacctttga
    24781 ttatgtagtt acgtttccat tcttgactaa aaatgtttca aaaacgaaca aaggtgttaa
    24841 aaggtaaagt gatcaatact actggtcaaa ataagaggtt gaaagggaat tgaaaaataa
    24901 ctacagataa tacttttata aacgtacgtg ctttgtggtt ctgtagttaa ctatataact
    24961 ggaattaggg aatggtatca atttgaaaag aaagattcta ccaatttatc atatgttaaa
    25021 aatggaaaat caacagcagt tctttcggaa accttacaca aaaaccgatt attgaaaata
    25081 gccgaaaaac gtttttcggc tattttttta attaagtttc cttcttctta ccttttccca
    25141 ctattccgtt tcacatcttt gatgatcttc agcgcacgtt ttcgtgtttt gatatttggg
    25201 cttttcaagc ggcggtatgc acttgagata tctgtttttt ccatatggac gacccacttt
    25261 cttctttgat tgtagtcatg ctaaaaaaca caaatcgaaa caattacgat ttgtgtttta
    25321 aatggtatca aaagttaaaa tgaaagtcaa cttgttaaaa cagtcgatca gactagctga
    25381 ttgactccta tctctggaaa taaattttca ttattacgtt ttagctacct aaaaaaggcc
    25441 ctcttgtccg ttaactttaa tatctattgt tatagctagt ttacctattg aacgtactgt
    25501 gtgttcgata gaacac
//
LOCUS       DACJGB010000038        25388 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.038, whole genome shotgun sequence.
ACCESSION   DACJGB010000038 DACJGB010000000
VERSION     DACJGB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.038"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(84..785)
                     /locus_tag="IYI34_001624"
                     /pseudo
     CDS             complement(84..785)
                     /locus_tag="IYI34_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354515.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
     gene            complement(785..1567)
                     /locus_tag="IYI34_001625"
     CDS             complement(785..1567)
                     /locus_tag="IYI34_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559495.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            complement(1584..3341)
                     /locus_tag="IYI34_001626"
     CDS             complement(1584..3341)
                     /locus_tag="IYI34_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAR0559496.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            complement(3316..6147)
                     /locus_tag="IYI34_001627"
     CDS             complement(3316..6147)
                     /locus_tag="IYI34_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAR0559497.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            complement(6144..6911)
                     /locus_tag="IYI34_001628"
     CDS             complement(6144..6911)
                     /locus_tag="IYI34_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR0559498.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            complement(6944..10063)
                     /locus_tag="IYI34_001629"
     CDS             complement(6944..10063)
                     /locus_tag="IYI34_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAR0559499.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            complement(10080..10367)
                     /locus_tag="IYI34_001630"
     CDS             complement(10080..10367)
                     /locus_tag="IYI34_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559500.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            complement(10421..10789)
                     /locus_tag="IYI34_001631"
     CDS             complement(10421..10789)
                     /locus_tag="IYI34_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559501.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            complement(10835..11347)
                     /locus_tag="IYI34_001632"
     CDS             complement(10835..11347)
                     /locus_tag="IYI34_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HAR0559502.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            complement(11358..11750)
                     /locus_tag="IYI34_001633"
     CDS             complement(11358..11750)
                     /locus_tag="IYI34_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HAR0559503.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            complement(11750..12088)
                     /locus_tag="IYI34_001634"
     CDS             complement(11750..12088)
                     /locus_tag="IYI34_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HAR0559504.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            complement(12072..12398)
                     /locus_tag="IYI34_001635"
     CDS             complement(12072..12398)
                     /locus_tag="IYI34_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559505.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            complement(12395..12724)
                     /locus_tag="IYI34_001636"
     CDS             complement(12395..12724)
                     /locus_tag="IYI34_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HAR0559506.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            complement(12736..12999)
                     /locus_tag="IYI34_001637"
     CDS             complement(12736..12999)
                     /locus_tag="IYI34_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559507.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            complement(13017..13940)
                     /locus_tag="IYI34_001638"
     CDS             complement(13017..13940)
                     /locus_tag="IYI34_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAR0559508.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            complement(13953..14555)
                     /locus_tag="IYI34_001639"
     CDS             complement(13953..14555)
                     /locus_tag="IYI34_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HAR0559509.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            complement(14682..15608)
                     /locus_tag="IYI34_001640"
     CDS             complement(14682..15608)
                     /locus_tag="IYI34_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HAR0559510.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            complement(15613..17112)
                     /locus_tag="IYI34_001641"
     CDS             complement(15613..17112)
                     /locus_tag="IYI34_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAR0559511.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            complement(17125..18369)
                     /locus_tag="IYI34_001642"
     CDS             complement(17125..18369)
                     /locus_tag="IYI34_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAR0559512.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWVDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            complement(18356..18889)
                     /locus_tag="IYI34_001643"
     CDS             complement(18356..18889)
                     /locus_tag="IYI34_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HAR0559513.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            complement(18916..19140)
                     /locus_tag="IYI34_001644"
     CDS             complement(18916..19140)
                     /locus_tag="IYI34_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559514.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            complement(19147..19359)
                     /locus_tag="IYI34_001645"
     CDS             complement(19147..19359)
                     /locus_tag="IYI34_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559515.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            19721..19900
                     /locus_tag="IYI34_001646"
     CDS             19721..19900
                     /locus_tag="IYI34_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAR0559516.1"
                     /translation="MYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQTINSI
                     KNGITKDSQVIDVTK"
     gene            complement(19893..20234)
                     /locus_tag="IYI34_001647"
     CDS             complement(19893..20234)
                     /locus_tag="IYI34_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559517.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(20533..21000)
                     /locus_tag="IYI34_001648"
     CDS             complement(20533..21000)
                     /locus_tag="IYI34_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HAR0559518.1"
                     /translation="MVLFDVKKYETPEAKDVDMDRTKHNVGVFLSAYLSARCRIGQPR
                     EPKVTASYSLVPPSTANNTFEAERMMIEREEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(21592..21879)
                     /locus_tag="IYI34_001649"
     CDS             complement(21592..21879)
                     /locus_tag="IYI34_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559519.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            complement(21876..22085)
                     /locus_tag="IYI34_001650"
     CDS             complement(21876..22085)
                     /locus_tag="IYI34_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559520.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            complement(22082..22279)
                     /locus_tag="IYI34_001651"
     CDS             complement(22082..22279)
                     /locus_tag="IYI34_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559521.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            complement(22276..22602)
                     /locus_tag="IYI34_001652"
     CDS             complement(22276..22602)
                     /locus_tag="IYI34_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559522.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            complement(22599..22880)
                     /locus_tag="IYI34_001653"
     CDS             complement(22599..22880)
                     /locus_tag="IYI34_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559523.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            complement(22893..23111)
                     /locus_tag="IYI34_001654"
     CDS             complement(22893..23111)
                     /locus_tag="IYI34_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HAR0559524.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            complement(23108..23419)
                     /locus_tag="IYI34_001655"
     CDS             complement(23108..23419)
                     /locus_tag="IYI34_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559525.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            complement(23596..23910)
                     /locus_tag="IYI34_001656"
     CDS             complement(23596..23910)
                     /locus_tag="IYI34_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAR0559526.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            complement(23910..24215)
                     /locus_tag="IYI34_001657"
     CDS             complement(23910..24215)
                     /locus_tag="IYI34_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559527.1"
                     /translation="MSVGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(24212..24373)
                     /locus_tag="IYI34_001658"
     CDS             complement(24212..24373)
                     /locus_tag="IYI34_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAR0559528.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            complement(24388..24747)
                     /locus_tag="IYI34_001659"
     CDS             complement(24388..24747)
                     /locus_tag="IYI34_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAR0559529.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(24744..>25388)
                     /locus_tag="IYI34_001660"
     CDS             complement(24744..>25388)
                     /locus_tag="IYI34_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR0559530.1"
                     /translation="SLKARALSFFQNGSVLTDKTLFKCKMENYHVVDQETKIALERAK
                     SFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILFISYQELLEQIK
                     FSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTNNTLNTLLEARQ
                     NKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIKPVKGSIA"
BASE COUNT         6856 a         5520 c         4040 g         8972 t
ORIGIN      
        1 ctataatcat aattcccgaa gtcgatgctg ttactgtaca tctttttcag cttgccgagg
       61 tcgccgattt gctgattggt ttgattcatc ttgtcttcaa ctgctttaag attgttttgt
      121 gccgatgtaa cgttttgaga aacggtatca atcttagttt gagtagcttg tagttgctct
      181 gttacctcgt caacagtttg atcaattgct tgtgtggctc cctctacaaa ttcatttatt
      241 tgttggtttg ccttttcaat cgtagaatcc acatttgtta atgctgcgtt aacagcatct
      301 gttgcttctt gtttgactcc atcaagtaat tgttgaaagt ctgcaataaa gtagtctgct
      361 ttatcttgcg ctagtccgtc gattgctgaa cgcttcatac ggaaggtgaa gcctaagtta
      421 tcacttgttg atccgtctgg atactcgatg taaacataag cttccaccat accgttataa
      481 gctttcatcg cttctggcaa gatatatacg acctgtccat tcaagaagct ttcagtgatg
      541 aggtttttcg tgataaaagg gattggctct ttcgtgatca ttccatctac ttcatcatag
      601 ataaacatca acaagcgcaa gttagctcct agcaagtccg ccggtgtgcc gttctgttgt
      661 tcgacgttga actctaacgc tatttgatta tcgtatgatt taaatacaag ccccgttgct
      721 tgcaaatcgt agtctttcgg ctgtgtaggt actttgatag agcctttttt gatgacatgc
      781 gccattattt cacaccttca atcttagtta ttttcacgtt gttgctcatt gcgggattgg
      841 aaacattgcc ggatgaaata tctaaagctc tgccgtttga catcgttatc tttcgcgctt
      901 cgattgtcag ctcaaattcg atgaacgtca ctcccgcgtc tccattccat aaattcgctt
      961 ttgtaattcg cgcgtatctt tgacgtttca cttcttctac aaagtctccg tcaccgtctg
     1021 tgtagtggat acgaagcgtt tggtaacgga aagtatcatc tggcagattg actgattgtc
     1081 cttttcttaa ttcgccttca aaaagaacag aagtagtgta aacgcgccgc cagcctaacg
     1141 agttataacc ctctaccggt tcgctgttat gtgtcatttt gatgtaaatt tcgccgctat
     1201 aacgagcaaa agcgacgatt aattttctcg aatcgtgatc cgcgaatacc atcatttcaa
     1261 caaaactgtc atcttcaatc ccgtcagggt tgtctgcgcc ccatccggct ttggttgcgt
     1321 atttgccggg cggaatgtct aaaatgttcg gatatttttg tggtagctta taatcgcgta
     1381 accaacgccc acgcgcttgc ataactgttt ccggtgtagc aaagcccgcg ttgctttcag
     1441 tagctaggac gtgcgcgtcg tgatccgagc cgtcgtaatg gatagccgct tgacgcaata
     1501 accatttcac agcctcttca taactcattt cttcaaaaat atttggtttg ctttgaaaat
     1561 ctaacattta attgttcacc gccttaatag ttataagtga agtctctcgc gcgccctacg
     1621 cctttgcttg tcacttttct tgttgctgtg tcaacttcta ttttataaaa cgctaattca
     1681 tccggtgtat caacttggct agctgtatga gagaagccga cgtcgcaaag aatcattttt
     1741 actgttccaa acgccccttc gacttgttcg tgccaatgcc cgcagaaata acctactaca
     1801 acgccggcgc cttttgtatt cattgcgaag gtctttaatc cgccgaacga accgtcattc
     1861 ggttgtccta atttagacca atcgatagtt accggactac cagatttaaa cccttctatc
     1921 aacgtgctaa tcatgttttc attgcgaacg ggaaacttgc tgagacctaa aggcgtatgt
     1981 cctactaacg ccacatgata gtttctcgga acgttcacta accattctcc gaaagcgtta
     2041 atttgtttgg cactgaaagc tcccggcgct gtatcgctgt aaccgtccac gtacttgtcg
     2101 cctgtgccgc cttcataaaa atcacaagta tcgaaacggt aaattgctgc gtttttatct
     2161 gggaacaaca cgccaccgta caaaccgttc caatactctt cgaagtcagc gttgcataac
     2221 attccctttc gcttgcgcca cgcggggtcg aaacaagcgt cgtgattccc tttgcagata
     2281 ataaccggtt tttcttgtcc tgctaccgct gcgttagtga agcgtttgag cgtgcctaac
     2341 atggaatgtc gcgcgctcca ttcatcaata atgcctatat cgctgccgag tgaaccaagt
     2401 ccgccgtcga tattgtctcc gccgtaaatc atcacatctg ttttgttccc taatttttga
     2461 aattgaggga ttgcgcgcca atgtcttaaa taagaagcat ctttgtaacc gacgccatct
     2521 atacgcaagt tgtgattatc tgcgtgaatg tctgtaatga aagaaaagtt aaatttgctg
     2581 ttattaacac tgttgacaac cgtattcaaa ttgcgcggca ctaggtcaac gtatttcatc
     2641 gtgtcgtaat caaaaaagcg tgttgtttct ctaatttgtg agctgccgac tggcacttgg
     2701 tatttttggt ttaaacgatc agctaatgat tcgtagtcgc ctttagcttc attcagaatg
     2761 ttaatgatca taccacctgg atcgatattt tccagtattt cacgattatc ttctaaccac
     2821 tgctcccagt catttttgcc ctgatccatg taatctttga attttcttag caaatcctca
     2881 aatgtccaca catagccaga gtcacgtaac tggcttctag atattccaga aatgactcga
     2941 taggtaaaat cctgtgtgct aaattgttca ctccaagttc catcaccatt aagtgatcgg
     3001 aaactgaaat gtgcggtgtt ttcaccaccc cattgccagt caggctcact taaggtgtaa
     3061 acaagccttg cttgcgctgg actgtattct tgtacttttt gttcaacagg ttggttttcg
     3121 ccaaattttg ttgtattaat aaaaaacggc actaggccct cgaatgtttt tagtttgcca
     3181 tgttccacca cttcaacaac gaacttttgc gttaaaacat ccccttgccg aattcgaacc
     3241 aaatttattc cgttgtttgg ttcggtggtg gataggacca ttttatgctg cgtttctgcc
     3301 atgactatcc ctcctttaga aatcgatgta gtctcttgca ttgtggaaat gacctgaaga
     3361 agatggataa aactcatcca taaattgaaa atgaagatgt tctccagtgg atggtcctgt
     3421 tgtccccatc agtccaattt gctgaccagc agttaccttc tgaccttttg agacgtctac
     3481 tcggctttga tgcgcgtatc ctgtatacat tccatcagcg tgtttaatca ctgtccaatt
     3541 tccataccag tcaaagtaat ttgcatcacc tgcaacaatc acttcgccat ccgctgaagc
     3601 aaaaataggt gtattaggat ttccatttac aaggtcaata ccgttatgaa attcttgtgc
     3661 tcctgtgatt ggagaagtgc gccagccaaa ttcgctcgtc actgtgattg gatccgcaat
     3721 cggcttcata tatccttttg atacaggaat ttccaaatct ttgaacttgt cataccattc
     3781 ttgtgaccat gccgtccgtt ctggatgtgg atcacgtgga cgttcaaagt tagccacgaa
     3841 tgcttgtgct gctgtgttga tatcggtcag attcatgaat tgtgtccatg tgtaaggata
     3901 agcgctagtt gcgatccatt gaccatttgg tgcatgccac atcaacaatt taaactgtgc
     3961 cgtgattgtg tctggatcat cactgatgcc agcctttgtc attaaattga tcatgtagac
     4021 acgtccgcta gttgcgccag aagaatcggt ccattgccat acaccataac cgaaccctgg
     4081 tgcgccattg ccctcatcag cggttggatt agcatctgat tctccttgtg catttccaag
     4141 taaggctgca gcagcttgtt gtgtaaaacc ggcacctatt gccgttgccc aaatttgcca
     4201 atatcgttta tcccgatcag tagttacttc tggtgggtat tgtccgttcc aaccgttatc
     4261 gttccctccg gaattgcctc ctccattagt gtcaattttt gtaccgttaa catagagttc
     4321 ttttacatct agccgtccat ctatggttat atttccttct gaaaaattgc cgtcaccata
     4381 aagattatat ttgcgcttat cagcagtaac atctgctgga atttgaaaaa taggattccc
     4441 tcgatcgcca ccgtccccag cattaatgga aaaaatatag tttggctctt tccagacagc
     4501 aaagccgtta atccctttag aaccataagt agatacaatt gagcccaagc gttctccatg
     4561 tacatcatct agtccagtag acttgacttg tttttcaaaa gaaagttctc ccccctctgc
     4621 cactaattgg aaatctttat cgtcaaatgt ttttaaagct accccttgca cgaggatacc
     4681 agacagaatg cctgcgcgaa taaaattagc attgaaagtt ccatccaacg tccaagcggt
     4741 cgtgctatca ccattgtgta catcttgaat tgtttgccac tcaccttttt tacattgttt
     4801 gaaagatatc cctgagttat tttggaccat aaaaaagcgt gatctaggaa tgttaggtcc
     4861 atccatataa acagtttcat agatttctct actatcacta acaccagctt caattccatt
     4921 tacccaataa atagaaccgc cattatctcc tgcacctcgc ataatgtcat cttgatattt
     4981 tccaatctct gtcgattcgt aaaatgtcat tttgctagat tctaaactat taatattatt
     5041 gacaatagaa gccgtttgtt ttctaacatc ttgtgttaaa ttatccccta gttcgatatt
     5101 cgtttgaccg gtaagccgat tgaatgtagt tttataaata cgagttttat agtgataacc
     5161 tttatcgtat ctgtgaatag tcactgtatt tcctatcaca tctcctccag tgacttcagc
     5221 tttgaattgt actaacggtc tagcagaatc gattaaggtt gaataagtat ttttaagtaa
     5281 atctgttgga tcatctatat catcaaacac tactacggtt tctcgttttc tcattgatcc
     5341 atctttttgt ggtatcccat acttttgagt tgattccgga tcttcaagcc aattttggcc
     5401 tttaggctta tctaaaggat caccattcga ctttttccat tcaacatcag tgaattcaat
     5461 tcttctaccg taaccgtcac caacctcttc gcctcgccca cgacctatca ttgaagttga
     5521 gattgagctt ctatctatct ctcttacaac tgttaatgct ttactaccat atacgaaacg
     5581 tgtattcgat tcttcgccaa tttgttcata tacttcgatc catttatcct ttattccatc
     5641 agaattcaaa gaacacctaa agacaaattc catacctaag gtttgcaatt ctttcaacgc
     5701 ttcttttaca gagacatagt aaaaagttgc agttactgtt ggtaacattg cttctacgtg
     5761 accaacgcgc caatttcctt cagtaaattc aatcaatcga tcaagaacat tttttaaggg
     5821 ctgcccactc ggcctaatat ctttgatgat gtaagcatct aattcatttg tcgcaaatcc
     5881 taaccctgta aactctaatg tttcaaatgg gtcgctaact ttagtaattc gatacaacga
     5941 aaaagacgac tcgttttcac gaatcgccat atatcttgca tcctctattt ctttatcata
     6001 ttttgtcgta acgtaaagag tatctttcat tagatcactc ttatcagaac taatttcttt
     6061 ttcttgggag acttcaatca aacttcttgt attttttctt ttaataagtt tttgcaagtg
     6121 atcaaagaaa taaactgtct cactcaaatt gtcgcccccc tatagaatat tttaagcttc
     6181 ccattattgc tagttatctt ctgaccttgc ttaagataaa agttctcaaa atcactttct
     6241 aaatcaatca tagaagtaca atcttctccg tttacagtta cctgctcatc ggaaaaatca
     6301 aaaaccaaca cgtctcctgt ttttattgcc gcgtcagtta tcgtgatatt ttgttctccg
     6361 tttgtaattt tgattgaatt attcatggat aaagtaactt caatttttct tggtgttata
     6421 ggaaactgta tcggatttcc aatatagcca tcactaacac actctttcgt atactttagt
     6481 ggatccgcac agaatacatt aaaactcgaa ataatcgagt tggagtctcc tggaacagta
     6541 tcagttgatg tatagcgacc gtagtaataa taatctaatt catcatgaaa cctaatttcc
     6601 acgtcttcat tccggtataa ataattcaac agttctttga atttaaactg tagtttttct
     6661 ggatctctat cttccagctt atatgttatt tttagtgttc ttgaaggtat tttctgattt
     6721 gtaatgattg aaccaatttg tatctcttgc tgttcaactt ctacagaaag catttctcta
     6781 ccttccaccg taagtgtttg ataaccatca atcaaatctt ctaaatacat tccatcgtac
     6841 atcatggcag acgtcggaag gaatcgttta gaactagtga gattaatggt tgtatctttg
     6901 aatgagtaca ttttattttc tcgttgatcc aaaatattcc ctcctaaaat tccagattaa
     6961 tatctgcacc ttcgcccata gcttgtgaaa tatcgtccat aaacaatcta aacgattgtc
     7021 ttccaagatt gattttaaat acagctggtt tagtagagcc acccatattt attttatgtt
     7081 caacttgtgc accaatgttt ttatttgcat ttttcaaatt tgcagctata tctacatcag
     7141 aatttgcatt gaaaagttcc gcgataaagt ctgccatact tccaacagta ttctgtacgt
     7201 cattgaatcc tcctgtcaga cctttattca gaccgttcat aatagcctga ccagctggaa
     7261 tcaatagctt tctatcgtat tggataggtc ctttgtgttc acgaatccaa tcaccaatac
     7321 ctccaacaaa atcttgcaca gatttccatg cattttgtaa cccttctaga aaactatcca
     7381 tgatagcttt tccggctgct agtaaatcga tatttttcaa gttatcaaac cagccagtta
     7441 ctctatcaac cgtgtcacta acagcattta ctaaattatc ccatgcctct tgagcaccat
     7501 ttactaaatt gttgaaagta tctatagtac cttgttttag gttttcccat ccctgaatga
     7561 tgttatcttt ggttccagta attaaatcac caatccaaga tttgaatgaa gtccaaatgt
     7621 ctttagcacc ttgaactgta ttgttgaata aatctatcgt tccttgcttt aagctgttcc
     7681 aactttttat aagagtttgc acgatattat taaccgtttg gaaaaaccat tgttttagat
     7741 tattccaaag ttcaattgca ccatattttg tatcaatcca agtttgaatt atggagaatt
     7801 ttagatttat ccacatttga attgcactgt atttaatatc aatccataaa ttagtaaaaa
     7861 atagtttcaa atcaatccaa atcatagtcg cttgataaac tacttcattc caaatattag
     7921 aaatactttg tatgaatcca gtccataaac taacggcact atttactatt gcaccgatat
     7981 attccgtaaa tatatttttt acagatgtcc aaatatttac cactgattgg acaagtgtgt
     8041 tccagattag atctaaatcc tctttcattt gttcgaagtc accagtaatt aaatctataa
     8101 taaacagtag tggcgcagct attaaagact taatgatttc ccaagcattt actattatgt
     8161 ttttaacctc agcaaatata cttgttattg ctttcactat attagagaac acatctgaaa
     8221 agctacttac aaatggacca atatatttta gtataaaatc aaaaccagat ttgattttac
     8281 ttgtaattgt tttccatgca gtagatatag tatttttaaa cccttcccat ttttcaccag
     8341 cacttgaaac taaatcatca ataactgata tgacaccttc ttttaaacta ttccaaatct
     8401 ctgaagcaga gtttgcaata cttgaccata aatttgaaaa gaactctttt gttccagtcc
     8461 atttgttttt aatccaatct gccgctttcc caggggcttc ttgaactgta gtccaaacat
     8521 tgtctgcgcc ttctttaatg gacttccata aattgttaaa ccattctcct gtagatttcc
     8581 atgcattctg aatccattct actgccgagc ttacagcaga cttgattccc tcccataagc
     8641 caatccaaaa gtttctaaaa tcttcactcg tattccaaag atagatgaaa cctacaacga
     8701 gtagtgctac cgcagctata accagaaaaa ccggatttgt aagaagaccc attgctttac
     8761 ttaatccact aaacaatttt gaactttcac ttaaagcttt aaaagctact ttcaccctat
     8821 tgatccatat aattactgaa ccaatcataa aaattaacgg tcctatagca gcgacaattg
     8881 ctcctatggc cactactaat ttttgagttg attctggagc acttacaaat ttttctacta
     8941 agccggatat ggcatctgct acttttttga tggatggtgc tagaatcttt tgaattacaa
     9001 tagctgctga ctcaaaagct ccaaacattt gctcgatgga agaattcata ttatcttgca
     9061 tggtccgagc catatcgtca gctgcaccat cagaatcttt cagagatttt gttaatttgc
     9121 ccaatgaatc aggtccttta tcaatcaaag ccatcatccc tgataatgat tcttgcccat
     9181 atagtgttac taaagcattt tgttgttgtt caggcgtcag gccttcaaaa gcttttttaa
     9241 gtaattctac ttgagttttt aaaggtttca ttttaccgtc agcatcataa aacgaaacac
     9301 ctaaattatc cattgtatct tgcatagcct ttgttggcct tgctaaccta gacaatgctc
     9361 ctcgcaacgt tgtacctgct tgagaaccct taatgcctgc gtcactcata ataccaatag
     9421 ctgctgcagt ttcttccaaa gaaatcccca ttgaattagc tacaggagca acatacttca
     9481 atgcttctcc catgtctcca acttcagcat tggtatccgc agcagcacga gcaaatacat
     9541 cagcgacatg tcctgcttca cttgcttcta aaccaaatcc tctcaaagca gtagcagtat
     9601 tttcagaagc tagagccaca tcccctccag atacagctgc taagtctaaa agacccggca
     9661 ttgctttcat gatttcttgt gcgctaaatc cagcagaagc taagttttcc attccagcag
     9721 ctgattcttt tgcgctaaaa gcagtttttg ctcctagatc aatcgcttgc tgtttcatct
     9781 gttcgaatgt gtcgccggtt gctcctgata tagcttttac acgactcatt tgtgcttcga
     9841 agtcgccacc aactttagca gctgctacgc ctactcctat aagaggagtg gtaatatact
     9901 ttgtcattgc ggcaccagta ccttgcatca ctttaccaac agcggttgtc atactatttg
     9961 aattcttttc aaaagtttta acagcatctt gtgcatcttt aaaagtcttt acaaatccac
    10021 tatctgtggc ttttaataag gcttcaacag aaaattgttc catgattctc ctcctttcct
    10081 caagagttag ctttagttag taagctttgg aattttttat cttgttttga aagttcggaa
    10141 actcccatga ttgaatcttc gattttttga taattgaaga attcttcaaa ggatcgatat
    10201 acaggaactg tctttttgcc tacttttttc tccgcttgga cttgctgatt tgcccacgct
    10261 aattcgtgaa tcaacttttc tttgtcaagc caagataact gggctgcagt catacgaatg
    10321 ttgtattcat ataacgtcat tctttcgata tctgagatat tggtcattcc caaatatcga
    10381 aaagaattga taagaatttg ttcgtatgcc agtgcagaat ctattccact tgttgttttt
    10441 ccgcttcttt caatttctga ttcaggtttc ggaccgctaa ctttcccgcg ttcgactccg
    10501 ccaattcttt taggacttca tcaaacaatt tttcaatgtc tttaacttca tcgatgtaat
    10561 catccaattc atctaatgta atagattcat cttcagttct atttgctatt tccagaactc
    10621 gtgataacgt gttaacgtta taagaacgta gctctggtaa gacttttgcg gagagtccca
    10681 ttccaaattc catatttcca tcgacgaaag gcatcacttt atctaattca cgtacaaatt
    10741 tagtgccaaa tttaaacgaa tattctttac ctttaatttt taatttcaat gtttttcatc
    10801 ctcctaaaat aaaaaagaga gcatctaagc cctcttatgc tcctgtcgaa gttgctttca
    10861 cggtatcttt gaatgcatat tgaacaacat cggcttgatc ttctgtcaag gttgcataac
    10921 catcttgacc aacaccattt actgcaaatg ataaacttaa ttcaacgtta tcctctgcag
    10981 cagccgatgg agtaaattca gacacatatg cttggtaata agtagctttg tacttatttg
    11041 cattatcatc tgttccctgt tctgctttat taatttccca aatttcaatg atatccccat
    11101 ttaataaagc ttgtttcatt tcatctacat gagaatctcc tttagcaact attgaagtag
    11161 ccgaaaaatc atattcaacc gggcttaaac tttgaacgtt tccatctttt gtcactgtag
    11221 agtctgaatc tcttgataaa ccattttcgt gttctgtttg aaatgccatt ttccaagcgg
    11281 cttcctgagt ttcttttttc aataagcgat aaagcaaaat gacatcaata ccttttaatg
    11341 cttccatgtt cttcctccta tctaattcta aattcaagtg tgacaaccgc ccgttttagc
    11401 ggtgtattcg ttgttgtgtc gtccatcact tgaattccac ttgcttgata atttaaagcc
    11461 caataatagc cttctgtggc ttctatcaat ctagcttcat taaaaagagc agatgccata
    11521 tctgacaact gctttcgttt cttctgtaat ccccaaacgg ataaaaccac aatcacagac
    11581 cctttaatgt cagttttatt tacttcatgg attgtctgag tgttctcaaa ttccacaaaa
    11641 ggataaccaa catcctctaa agttttgtaa tcgtatgttt tatatccaag tttgttttgg
    11701 gatattttaa aaagttcatc aaaaattgat tgatctcttg tcttaatcat catttcacca
    11761 aggctttcat ttcagccata aatttgactt tttggtaatt aaacgctggt ctaacataag
    11821 gctgtgccga cataaatcga gtgccatatt ctacataagg tgcataatct gctgtcggtc
    11881 ctacaatacc agttaaacca gcttctaaaa gattcatgtt tattgatctt cgtaagtaac
    11941 ctgtatccac tggcgcacct ttttgcattc gttcagtcat ttcagcagta ttacttttca
    12001 cgactttttg aacgtcatta agcgttgctg cttttttcag atgtcgcatc agctgatcga
    12061 ttcctttata ttcaagttgt gccttcatca agaaccacct cttgcacaat taaactattt
    12121 ctatatgctg gatttctagc tgttttttgt tgccaagtct ttccttcaat ctcgatatag
    12181 tcaaatgtag ggatagaaaa aagaggctgc gtcctaatga ccttcgcccc ttcttccaca
    12241 ctaccaaaaa tagtcacact tctatcagtg ccaatatctg tcacgtttgc ctctgttctt
    12301 gttctttctg gtttgccttc aacccactca ccgagatctg gatcatattt agagtcagat
    12361 gaacgtttaa caaatataat ttcatctgta aatctcatac aaatttaaac ctccctcgct
    12421 ttggcttgta caactcttcc tgatctttac gcttaaattc atcaatctca ttttgatact
    12481 ctgaaaaatc cgagtcagga aaagccatag ataaaccttc ttgagaatat gactgcatac
    12541 cttcttgacc aattctattg aatcttttca acgatacttc atatacaact gtttcaaatt
    12601 ctttaggaac ttcttgcgta tttaacaagg ttttcatacg ctcattcgtt cttcgctcaa
    12661 taacttcaag cttttcatct attgttcctt taagaagttt tttaatatca tctgcaatca
    12721 tcatattttt acttcctaac tagcaggttg acctgtcaca ttgattgaag tagtgaattc
    12781 tccagaagta aatgtgaatg ttgctgaccc ttctgctgca atcgttccat caaaaccacc
    12841 attttcattt ttggtcactg ttgcgatagc tccatcactt gaagttgctg tagtagctgc
    12901 aacaacagca gctgcatcgc tagcatctgc aggcacagct gaaatagtaa atgttttagt
    12961 atcgcctact ttaccggtcc atgtcttttg atttggcaca ataccggtag caggcgttac
    13021 gcttttgggg aaatcttccc aaatgcttca tcttttacaa tcataaatcc aacatccatt
    13081 gttgcacgca aagcaatcag ttcttgctca aacaagttaa ctggggttcc atcttcatta
    13141 gttaaagtag acaattgggc ttcttcagaa atcttaaatg aaatattata tgggatccca
    13201 taaaacatgt aattaaagtc tccagcgtaa agtgttcctt tatctaaaga cttaaggtct
    13261 actactggta atccgtcaat tgtattagta gagcgatcat aaataaactc aacatttgac
    13321 ccgactgttt gagctgcaga acgtaattct gtacgatttt ttcggtttga aataaacgca
    13381 ttaggttcga attcattttc tgctaacttg tcttctaagg ctaggatatt atcataagtc
    13441 aatccgcctt caaccacatt ccccgcacta ataactgatc cgtctagtga ctgaggaaat
    13501 gggttttctt tatttaataa ggcagctgca tcaaattttt tatagaaagc ttcagcaatt
    13561 tttggctgca tctcctcaaa gaaatctgat aatttataat ttaaatattc acgagaaacc
    13621 ggaagaatga caccgagttt ttttgcagtc atcgtagctt gcatccattt aggtttagac
    13681 gttttaattt tttcaccttc acccacccag tatgcgcctg gtccttctgc aaagtattca
    13741 aatttctttt ctttgtcagt catttcttcg tattttgcta actgcatgat cttagaattt
    13801 tccataactt cactcaaaat gagcgtatta tatttatcag gaatttttcc ctctttcgtt
    13861 tcatatacca agacattatc tggatcccat gtttgagcaa acatttgcaa gttcattggt
    13921 aaaagttgtt tctttttcat taagttttcc tcctatttga taattcgatt tttagcagct
    13981 agtttagcca ctgtttcttt agtatttttc gatgctgtaa attgtccacc ttcatttggt
    14041 ggtgtttgtc ttgcgttttc tctcttaatc aaagaagcaa agttagtgat gactgctaca
    14101 gcttgttttg tggcatctgc atcatcagaa acaatcagac caagtaaatc atcatcgtgt
    14161 ggtaaatttg catctgtcag cattttagaa gcttctttcg tcatttcaga tagtgcctgt
    14221 ccacgcttta attcagcgat ttcagcttct ttttgctcca actcatgctg tagtttttct
    14281 tccgcattca tttttgccag ctttttagct tcttcttttt ttgctgctag ttctttttcc
    14341 cacgctgctt ttgctttatt cgtctcagca gcgatcattt tcgctacttc atcacgagaa
    14401 aatgttttgc cagtattgtt ttcttctttt gcttcgggcg gtgtctcttg tgagccagct
    14461 ggtaggtttc cttgttgtcc ctcatcacca gatccaccat ctcctggttc agaaaaaaat
    14521 tgtaagttca ttggcataaa taaacgtttt ttcatgatta atcctccacg gttacgccgc
    14581 tacccgataa atttgaccag ttacgccggt cagccgaaaa tagctttctc tttaacgcct
    14641 gtaagctgta agaaggcaca ataaaaagcc gttaatttgt gttaacggct tgatattcgt
    14701 tctatataag gtgctgtact gcatcgacaa aatggatgca tattaggagc gttacttcct
    14761 ggctgcatat cggcaacatc aaaaacttga ttatttaacg gtatacatag tttgcacgcc
    14821 gttggttctg ctatatagat gtactgggta atgcctgcat ctctataact tcgttcttga
    14881 atccctacct gaactctagt cgtttcagtc accatcaaac gttgagtgtt gaacttagtg
    14941 ttttctcgtc cttcagctgt taaatattct gccaatttag atgcaagttg cttgggattt
    15001 ttccccatcg ttatacttct gactaacaac ctatctaatt ctgatttcaa ttcagattga
    15061 tacatccata agcgatcact aaaagacacg tcatcactca aaaaggagct gtttattact
    15121 agttctatca gctttgcata tccacttgaa gcaatagtca tttctaaaat accggcttgt
    15181 ctcttcaatt cagctaaacc agcttttgtt aattcattcg aaaagtactt atccaattca
    15241 ttaaacagtg aaatcaattc aagcccgata ttagctttta agagctctaa tctatttaca
    15301 cgcatcgtaa gattgtatag ctttaattct tggtttgctg taggagaaaa atctttctct
    15361 ttgacatact tctttgcttt gcgagcgaat gctttgacat ccatttcact agcacgcttc
    15421 atcgcttcgc tacgagtgat tttctggcca ttggagaaac tgtcccactg cgcgtctatt
    15481 tctttttgta tcgcatcttg tgcgtattgc atgcgacttt taatttctgc catgcgcttt
    15541 ttatcatctt taatttgttg cttttgccat tccttttccc gtttgatcca atattcttgg
    15601 gagttcattt aatcaccccc ctgtttcatc ttttttgttg ttaattactt cttcaccatc
    15661 tgaatcaaaa atgccaatct gcttttgcgt ttctttattt actcgtttca actctgcctg
    15721 tacatctgga acaaaagaag cgagtcctaa gatcgtctct tgactgagtt cagctccagc
    15781 atcaaccaaa gatttcaact cttccagaat ggctttaggt agattaggcg taaatattac
    15841 acgtaagcct ttcaaatcgg agttatccat ttcagaaata cttgatttta ggctaaataa
    15901 aagacgataa cgccgcataa ggcctttttt gaatagcctt tgctttgttg ccgtcatttg
    15961 ttcaaatcca aataatttat atttcattgc ttctcccgat tgcactccgg aaaaattgtc
    16021 atcagtaaga tcaggaacca tggatatttc gtgaatatcc ttgcgtactc tgtatttgta
    16081 tgcttctaca ccgttcacat catattgttt gtaaatatat cctgcagtca cacttgtttt
    16141 attaccgttc acatcagttc cagactcaag caaaagcata ttcgcttctt tctgcttgat
    16201 ggcgtcctct gtggatagtc ctgctgcttc aatatcacca ctaataacta gaagagcatc
    16261 gtttagatca gtcatataat tggcggtatc agactgccct gcatcgtata gatcaatcaa
    16321 agatagtaca tcttcataca agcccatccg aaaacgatta ggagaatact ctgtaatagg
    16381 tacctcttta tattcatgcg gttcatcctg gggattcttt aactcaattg ctgttagtgt
    16441 cgtctcatca taagtgatac ttttttcttt tgtgtatacg attggttgaa tgtactgttt
    16501 atcagcatcc ttgttgaatc ttgtcttagg ataccgtaca gccaaaatag gctctcgttt
    16561 tactgtagta tcatatacaa caaacgtttc aaatacatta gccaaatcaa cataatctgt
    16621 atcatctgaa tctcgataga taatctcata ggctctccca tacttatcca tatcaagcca
    16681 gagttcagca tttaacccat ctatgtcatt attagtatta aactcttcga tttctttttg
    16741 ttgatttgtg tcctcgattt gcacttttat aggattgcct gtgttgtacc caacatcaaa
    16801 cgtacaaaga acttttccaa agttatgtgc tgatcggtga tccgcttttt ctttttctct
    16861 gcgcctacgg ttatccatga tatttgtatt tctagcttta taataatcat ctaatacact
    16921 tagccttttt acctgatatt catgatgatg ttttatcatt gctgctaaag tatctaaatc
    16981 gttaagtaaa tcttctgctg agctaaatct atagtgaaga tttgagtcta cgctaaactt
    17041 tacataattt gtgttcacgt cattagaata atgtatatca gctccgtatt caaactcgtt
    17101 taccttatcc atttttcaca ctccttaaaa catgcgttta atacgtttaa tcttttcgtt
    17161 gacattaact tttggtttct taagaagctc atgagtatat atcgcatatc gaacggagtc
    17221 tagcacatca tcatattctt ttattggctc gccttttttc ttgtcccaaa tgtattgata
    17281 aatttcatcc cgaaatttca tcactctatc ttgacagata aacaatttat cttgtttaaa
    17341 tctcttggcg acttcctcaa ctccagataa ccgtgcttta tgagcatttt tagcattaat
    17401 cttttctctt acaaacctag ccacatgctc cggtctcgca gagtcacaat aaaaaggtac
    17461 tcgtaagccg taacgctctt gaatgccttt tgccacatct acccaataat caatctcttc
    17521 aaattgagta gcatgttctt cgattaaata agctgttccg tcatccgttt cacctataac
    17581 tacgattgaa ccccagtgtt catatcccca gtcaacacca caataaaagt tgctcaaagg
    17641 tggtaagtct tttgactgga tgtagtgctt gctagcatca aaatcacgat aaacgacacc
    17701 ttctgcagaa acccataaac ctttgatatc acgatcataa aacatgccgc ttggtgttga
    17761 ttctttaata ttgttacgat atcgttcaga taaaaatgta ttatcatcta attcaaagtg
    17821 gaacgattga atatttttgc ttgaattgtc gatatactct ttctttagcc agtgttccgg
    17881 gttgtcaggg tttgtatcag ctagtattct cgctcctgtt cctgaacaac gtgaaacgat
    17941 ttcagcaaat acttcttgtc tagctagcga tgcctcattg atataagcgc catatgctgt
    18001 cataccacga attgcgccaa caccgccaat gtttcctgta taagcttgta cgaccttaac
    18061 gccaaatagc ttaaagtttc catgtttatc gaatttaggt tctatgctgt acatgttgta
    18121 tagttcctgt aagatgttct tttggattgt tgcacttgaa actcctgcta ggatatacat
    18181 tggttcctta atgtcttctt catcggcaat ctttcgcaca cgtcgcaatt caaacaagaa
    18241 caaatcattg tttatttttg tttttccaga acgttttgct ccatgtaata aagtaataaa
    18301 ccaatcattc ttaatggttt tatttaacac gtcgatttgc tttgggttat aaatatcaac
    18361 tagtgccatc taattcacca ctaatctttt ctaacaactc atcaagtttt tcttcggtag
    18421 aacgctctga gtttatctgt aattgttcta attttgcttg cattaatttt atttctgttt
    18481 gagctttttc taattgcgtt tgtgataaca gcgatttata cttcaaatac aaatccaagg
    18541 ctttcaattt gctttctaaa tctggtgtat attcatattg catatgttta ataaccttgt
    18601 ttttctccaa gcggtctatt tgcttgctca cgcttatttg tacttctcct tgccaaatat
    18661 ctatcaattc atttaaagcg tcctcggcac ttaatttgcg cttcttttcg ataggattta
    18721 gccgttcatt aatatactgt attatgttag gtttagttag gttttcctta cctattactc
    18781 ttgctgatcg tttgctataa cctgctttaa tggcggcttg agtagcatta ccaccatttt
    18841 ttatatattc atccgcaaaa gctttttgtt ttggtgtcaa atttaccaca ttcaagccac
    18901 cacctttcaa ttttattaac aatatctctc aatatttgtc tgtatgttct gctcactaaa
    18961 atatccatgg ccgcagtaac gaagattgta cttatcaatc tcttttggcg tagcttctct
    19021 ggtcatttca atgatggagt acttcttttt aatctggact gactggacaa ctctaattgg
    19081 atcatctgcg tttggttgcg gatatttatt tgataatgat acataccagt agttcctcat
    19141 tacgtatcac tctttctgtt ggttactggc agaaaaggtt cgcatcataa attccatagc
    19201 ctgtgcgtca ttaaatcctt gcagaataag ctgatcgtag aaatacttag cttgttttgc
    19261 gattaacgct aagctttttt gagtttcgta gaatgtcgcc tccattgttt tgtttgcttc
    19321 attcgtttta aacaactctc ttaattggtt ttcgttcatt tttttactcc ccttccaaaa
    19381 taaaaagacc actcgatgag tgatctaata tgtaaaaact acacctcata aacgagatgc
    19441 agttatagtt ctatcaagca acctacaccg attccatcga ttactatcga cctcgccttg
    19501 ctcgtgtact ttgagcgccc atttccaacc ctcagttgcc aaagtcactg gcaaggaatc
    19561 gaaccttgca tggttgccga agcattgacc taacacacat gcttagcgtc taccctttcc
    19621 gccacagtga caaattaata ttgtgaaaat aaatactaag tgtataattt tatttatcag
    19681 cgagtggtcc gctgaaataa attaaaaggt ggtgaaaaat ttgtactttg ttataagaga
    19741 agcaactaat ggccaatatt acttcgttat taagtccgat aacaatgaag tagtagcaac
    19801 tagcgaaaca tacttgacta aatattccgc tgaacaaacc attaattcta taaaaaacgg
    19861 aatcaccaaa gactctcaag ttatcgatgt gactaaataa gacgactaac ttcatttgct
    19921 agggagttca tttcagctgc tttttctacc aaagcagttg agatactttc tagcttttct
    19981 gatttaacag aattaaatac tggcgtcttg atactaatcg atacatcttg acgaggatca
    20041 ggactcgttg ctctattttt ttgcaccact gatctgtact tttctactga atgatactca
    20101 attacttgtt ctaaatgttt tttgtactct ttacatacag cttctttttg gatcaattct
    20161 aaacgttgtt gctcaataat atcaatcaac cgttcccgat ccataccttg atactttatt
    20221 tgctcgactt ccattcgttt tccctccaat acataaatta ataaacagca acggatgata
    20281 gataataaga acaatttaga aggagttgga attcacatcc ttattcttaa tatttccgtt
    20341 gctgtctatc gaagcttaat tgtgaaacaa taataaaacg atgttccttt tattattatt
    20401 ttgtctcaga cctatcacta atctttcgac actatcataa taaatcattt accaggtaag
    20461 tgattggtat aaaaaaggta taaaatggaa accaaatggg taatcaaagg gtataaaaag
    20521 tgtaaaaact ggctacttaa aagcaaccag ttctaacgat gaagcaaatt ggatgataat
    20581 tctgtttgat tctactttaa ccgattcttc gctagtatta ttcctttgag cagttacata
    20641 aatgggcaga ccattgatat aacgatcata gaatatcttc ttgcgccttt ctgttacatc
    20701 aggtttatgc ggatgctgaa ttgctgaata gccacgaaca aataatttgt gcaagtactc
    20761 aaattcttct tgcgcttcct ctctctcaat catcattcgt tctgcttcaa atgtattatt
    20821 tgctgtagaa ggtggaacca aggagtaaga tgccgttact ttaggctccc gaggttgacc
    20881 tatcctacat ctagctgaca gatatgctga aagaaaaaca ccaacattat gttttgtgcg
    20941 atccatatcc acatcttttg cctctggtgt ctcatacttc tttacatcga aaagtaccat
    21001 ccattgattc ctccatttat gatataataa ttgtgtcaga attattaatt aaggtcggag
    21061 gaatccggct ttttttatac ccatgcttat gctaagcttt tcaagtagcg aggttgcact
    21121 cgttacccat atacatgttg agctatctgg cggaaaacag atggctcact atttcaatat
    21181 tctgctaagg acagccagtg gtcggctgtc ttttttatat tttaatgaga agccttactt
    21241 atttttttat ctttattacg aaaaatgata ttatctattg ataataaagc gtataggaga
    21301 tggttgagtg actatgtgga gcatgctgtt attttggatt cccgtttgta ttggtatcgt
    21361 cgcattttgt tactttgtca aacactctag aacaaataag ctcctcatgt tatctttttt
    21421 acctatagta ttttttattg tacaaattgt taaatatacc tatattgaat cgcaagaaat
    21481 attcattttt tatgtggtag gtttatttat ctctgtggtc tttttcataa tgatactttc
    21541 ctatttttat aaaaaataaa tttttctctt agaagtacat tttttcgctg tttatatagc
    21601 cttcatatcc accaatctcg ctacagctaa attctctttg cttttcgcta accacttatc
    21661 acattccatc gtgttttcaa tacgaatgat cgctgagtga ttatagacgt gttctacata
    21721 tccacgaaat ggatagatga acccttctgc ttcgcagcga accatgtcac cgactttgaa
    21781 ttttggtttc ttacgttttt taggattctt tgtcggcata tctagcatta aaccgccgat
    21841 accatgacta ctagcgtaaa atccgtcttt taatttcatc tttctacctc ctcatgatcg
    21901 acagtgacca gttcatatac ttgtgttaat ccaccaagtc gtcgtgaaac ttggttagct
    21961 tcttcgaggc tatcaaacca tctcgcactt tggagtatgt ctacaatgaa taatgtgtta
    22021 gcccctatag agcgttcatc acttctataa aatttattcc cgaactttac tacataaatc
    22081 ttcattctgc ttcctcctca atcgtccaag catttgtaaa tttctctaag cttattagtt
    22141 catcttcatt taaatatccg taaccatata aggattctaa aaggtcttct tttgaatagc
    22201 ctctaaaatc aagtctgaga ccgcttagga tgtatacata ggttttattt ccataaaact
    22261 tgattgtgta tgctttcatc ccacttcctc ctcatcgatc tctatctcga catctctgcc
    22321 ttctaaaatc tgtttggtat caatataggc tacgagtatt tttcgatttt ggcttaaagc
    22381 aatatacgga tcaatcaaag caacttctgc gtgtagacga taataagcaa tacaatctat
    22441 ttctaaaaat atcaaacgat caatagcatc attaatcata aaatctgtaa cgttttctgc
    22501 tgtatttaaa ccatctgcat atttaggcag gacttcatta ttgatgaact cagctacatg
    22561 ttttggacat aaatcagtca gttcttgttg tgtatagttc atttgctgtc ctcctgatta
    22621 atatatttcc tcaaactacg tatcccttta ccacgttttt ttgttttgcg ctcaatccac
    22681 gttcctgtag ttccccagta gtcaataatt ccattatggg tattaattgc aaattgatga
    22741 ttgtttggat aatgagtaaa ttcaaatccc aaggtttcaa tatttttagt tgctgaattg
    22801 cccatctttc tgacatgttg ttttctacgt tcttttagat gcggtttaat atctttccag
    22861 tattcgccca tatcgcccat ttcgctgtcc tcctatctcc ttgtccacaa taaaaaccac
    22921 atcaactctt ctgtcgtacc gtttaaataa agatttattt ctgcaccaat cgcaactaaa
    22981 attccgacaa caaacaaaat aatccctact gctgtatatt ttttgccttt tgcactctcg
    23041 taaatgaggt atgctcctaa catcgctagt aagccgccaa ttacacacca aaatattgtt
    23101 aggctgatca tttgctgtcc tccaaatcac tcgacttcac gaatacacca tctaccattt
    23161 ttcctgtgcg tcctttgatt tcgttgtatg cttggttcag acactcgtac aaatccatat
    23221 cattttgcat agctaaaata atcaaggtta ctactacatc tccaattccg tctcttaaac
    23281 catgttcatc ttttctagct agagaagcgg caacttcccc gatctcttcg atcgttttta
    23341 acatttgctt gctggaatca gcttgatcca atcccttatc tttagcccac tgctctactt
    23401 ttgtgattag ttcgtccatt attcattctc ctttatatat ttaagttgga taatacaact
    23461 tgtaacaaat gaagctaatg ttaaaattgt gccgattggt gttaaaaaac cgctttttat
    23521 cgcattaatt aatatagcta caaatatcgt tatatagaat aaaaagtgaa ttgacgctaa
    23581 cgttgaatta aacatctagt cctcctcgaa atactcattc agtatctctc tatacttttc
    23641 tacaaatttg aaacgatcct gatgaagttt cttgctccaa tttgtttgcc gatccagctc
    23701 acgcatctga tcgaaccctt tttgaatttc gttgtaataa aattcaatgt ttgctgctgc
    23761 tttccaatgc ctgctacttc gcactcctgc tcctgtttca gccatttcca acttaactaa
    23821 ttccgctcgt tcttttgctt ttttatcttt ctgaatcttc atcatgattt tcttgaggat
    23881 gatatcactg tattgtgtaa tgagatccat tatttctcct ccacatacct aaactgtcgc
    23941 ccctttgaat caatccataa gctcctagct ctatcccaga tgatgttttt gctcagtcca
    24001 gtaatttcag ataactgttc agctgtaccc gttactagaa ttcgatcacc atgccagatt
    24061 gcaatttttc tcggcgtttt ccgtttaggc ttttcagtcc acattgattt accgagcttt
    24121 tggacttctg caactatttc tttgtcttct tgccaattct cagaatgtgt cagttcaatg
    24181 attcgtttca ttgctgcttt cttacccacg ctcattcctc caatctacga atttcccttc
    24241 ttaaattcac tatgtgcaaa ttgattgcct ttctcgccgt ttcattgacc atcactgcct
    24301 ttatccgttc cagatcgtca atctcacgtt gaaggcttcg aatacgcatt tgaatcactt
    24361 cttctgttgt catgatggac cacctcgtta aaaacgctct tccttgaacg tattccgata
    24421 ttttttagct aatatcaacg gcacttgata ttgatgacag aacaactttg ccttgatctt
    24481 aaagtctttt gtctgcattc ctttgacatc tacgactttg acaagtttgc cgtttttata
    24541 aaatgtgaag tcgggaatat actcgatctt gcgatacttt tttccatcta gttcaaattt
    24601 cggcatcagc tcaaatcttt cctgaagttt tactttccag ccgttcgctt ctgcttgcca
    24661 caaggctaaa tcgtaatact ctgcttccgc gatagaatca aacttgatac ctcgatgaac
    24721 agttttctta ttacggtatt tattcatgcg atactacctt tcactggttt tatgcgcttg
    24781 tctgctgttt gttggaattt cagcgcataa ccttctgaat tcttaaatat tctagaaaca
    24841 attctttcgc cgtaggcttc tcttagttca ggaccggata agtttgttgt gatgatcgtt
    24901 gccttgttct gtctggcttc taagagcgtg tttaacgtgt tgtttgtaaa ctgcctacta
    24961 tttgataccc cgctacctaa ttcagctcca atatcgtcaa aaaccaccaa atcagttgtt
    25021 ttgatatcgg ctataagcga tccttcaatt tcttttctca gttcagcatt gttataagaa
    25081 aactttattt gctctaataa ctcttgatag cttataaaaa gtattttctt gtcataattt
    25141 gagcgctcaa gtatttccca agctgtcgcc attgacaagt ggctttttcc gcttcctgat
    25201 ttccctgata gaatgaaatg tgcaggatgg ttcagtaaga catcatttac aaagctttta
    25261 gctctttcta aagcaatttt cgtttcttgg tccactacgt gataattctc cattttgcat
    25321 ttaaacaaag ttttatctgt taataccgaa ccattttgaa aaaaactcaa cgctcgtgct
    25381 tttaagct
//
LOCUS       DACJGB010000039        25299 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.039, whole genome shotgun sequence.
ACCESSION   DACJGB010000039 DACJGB010000000
VERSION     DACJGB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.039"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..614
                     /locus_tag="IYI34_001661"
     CDS             <1..614
                     /locus_tag="IYI34_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAR0559531.1"
                     /translation="VFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVGK
                     YMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGTG
                     VFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPFI
                     LALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            611..1384
                     /locus_tag="IYI34_001662"
     CDS             611..1384
                     /locus_tag="IYI34_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAR0559532.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1381..1779
                     /locus_tag="IYI34_001663"
     CDS             1381..1779
                     /locus_tag="IYI34_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAR0559533.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1964..2635
                     /locus_tag="IYI34_001664"
     CDS             1964..2635
                     /locus_tag="IYI34_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559534.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2694..3134
                     /locus_tag="IYI34_001665"
     CDS             2694..3134
                     /locus_tag="IYI34_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559535.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3146..3814
                     /locus_tag="IYI34_001666"
     CDS             3146..3814
                     /locus_tag="IYI34_001666"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAR0559536.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3878..4501
                     /locus_tag="IYI34_001667"
     CDS             3878..4501
                     /locus_tag="IYI34_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAR0559537.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4557..4943
                     /locus_tag="IYI34_001668"
     CDS             4557..4943
                     /locus_tag="IYI34_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAR0559538.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5156..6547
                     /locus_tag="IYI34_001669"
     CDS             5156..6547
                     /locus_tag="IYI34_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAR0559539.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            6894..7541
                     /locus_tag="IYI34_001670"
     CDS             6894..7541
                     /locus_tag="IYI34_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAR0559540.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            7934..10579
                     /locus_tag="IYI34_001671"
     CDS             7934..10579
                     /locus_tag="IYI34_001671"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAR0559541.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            10889..12202
                     /locus_tag="IYI34_001672"
     CDS             10889..12202
                     /locus_tag="IYI34_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAR0559542.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            12192..12845
                     /locus_tag="IYI34_001673"
     CDS             12192..12845
                     /locus_tag="IYI34_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR0559543.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            12900..13592
                     /locus_tag="IYI34_001674"
     CDS             12900..13592
                     /locus_tag="IYI34_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAR0559544.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            13742..13942
                     /locus_tag="IYI34_001675"
     CDS             13742..13942
                     /locus_tag="IYI34_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR0559545.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14196..15875)
                     /locus_tag="IYI34_001676"
     CDS             complement(14196..15875)
                     /locus_tag="IYI34_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAR0559546.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15877..16089)
                     /locus_tag="IYI34_001677"
     CDS             complement(15877..16089)
                     /locus_tag="IYI34_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAR0559547.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16482..18212
                     /gene="efrA"
                     /locus_tag="IYI34_001678"
     CDS             16482..18212
                     /gene="efrA"
                     /locus_tag="IYI34_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAR0559548.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18209..19969
                     /gene="efrB"
                     /locus_tag="IYI34_001679"
     CDS             18209..19969
                     /gene="efrB"
                     /locus_tag="IYI34_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAR0559549.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            20064..20261
                     /locus_tag="IYI34_001680"
     CDS             20064..20261
                     /locus_tag="IYI34_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559550.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20414..20899
                     /locus_tag="IYI34_001681"
     CDS             20414..20899
                     /locus_tag="IYI34_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAR0559551.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(21003..21596)
                     /gene="clpP"
                     /locus_tag="IYI34_001682"
     CDS             complement(21003..21596)
                     /gene="clpP"
                     /locus_tag="IYI34_001682"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAR0559552.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21776..22303
                     /locus_tag="IYI34_001683"
     CDS             21776..22303
                     /locus_tag="IYI34_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAR0559553.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22394..23131
                     /locus_tag="IYI34_001684"
     CDS             22394..23131
                     /locus_tag="IYI34_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAR0559554.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23135..24052
                     /gene="miaA"
                     /locus_tag="IYI34_001685"
     CDS             23135..24052
                     /gene="miaA"
                     /locus_tag="IYI34_001685"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAR0559555.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            24054..25283
                     /gene="hflX"
                     /locus_tag="IYI34_001686"
     CDS             24054..25283
                     /gene="hflX"
                     /locus_tag="IYI34_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAR0559556.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8149 a         4304 c         5529 g         7317 t
ORIGIN      
        1 ttgtttttgc accgttagaa ggagaagacc tcgcttcatt tctggttggt gcggcaatca
       61 tgatgatcgg aatgacgctc tttttgtttg gagcagatta ttctatgatg gaagtcggag
      121 atctagtagg gaaatatatg atcaaaaaga aaagtttgac cgttttggtc agtttgggat
      181 ttgctatcgg gatcgtgatc acgattgctg aaccttccgt gcaagttttg ggacaacaag
      241 tatatgatat atctgacgga gagattggca gagtgttgct gattgggatc gtcagcgtcg
      301 gcactggagt atttctagcc tttgcgctgc ttcgggtcgt attcaagcta tcctattatc
      361 aattgatggc tataggctac ataggcgttt tgatcgcctc atttttcacc agttccgaat
      421 ttatgccgat tgcttttgat tcggggggag tgactactgg tccagtaact gttccattta
      481 tcttggccct tgccggaggg atgactagta tgatcaaaca aaagaaaaat gaaaatgaca
      541 gttttggaat ggtcggtatt gcttcgttag ggccgattct tgccgtgatg attttggggg
      601 tgatcttcca atgagtttct ggcagcaagt gatagatgga atcgaaatcg ttaccatgga
      661 agtcattttg gcagtggcac ctatcgtaat cctatttgtt ttaatgaata tcattagttt
      721 tcgtatgcgc aaaaagcgtt tctttgacat tatgaaaggt ttcttgataa cgactattgg
      781 gttgatcctt ttcctgcacg gagtcaatat ggcttatgta ccggtaggac agcatttagg
      841 cagtgctatt gcgggattgc cttacaatta tatactggtt ccccttggct ttttaatggg
      901 gttcttagta gggtttgctg agccggcgat tcatgtaatg atcaaacagg ttgaagaatt
      961 gagcgagggg agaatccgat cgaaaatcat gctgatggta atttcaattg ggatcggcgc
     1021 agcagtagga ttgtctatgt ggcgtttgct cgtcggattc tctctttatt atttcttgat
     1081 cccaggatac gggctagcat ttattttagg acgaaaagtg gacaagatct ttttagcaat
     1141 ggcttttgat aacggtggag tagcaacggg cccgatgtgt tcgacattta ttttatcaat
     1201 gagcgtggca atcgctgcac aaattgaagg aagagatccg atcattgacg ggtttggtgt
     1261 ggttgcaatg attgcattga caccaatatt atccacttta gtactaggat atatttataa
     1321 acgaaaagat gaaaaagaca gaatcgaaaa agaaagattg caaaaattgg gggaaagcaa
     1381 atgaaagatc ttgtagcatt gaatcttcac atgattgtga cgatcgttga cggaggaatg
     1441 ggcgaagatg ttatcgactt ttcaaaagaa gcaggtgcag caggcggaac gatcttacat
     1501 ggaagaggtt ctggtgtaca tgattcaggg aaatttttag gtttggacat ttcgccagaa
     1561 aaagatattg tgttgacatt agtacccgat agtttgacga ataaagtaat ggatgcgatt
     1621 ggtcaaggca tcaacatcga tattccaggt aatggtattt gttttagtat tgatgtagac
     1681 aaagtcattg gtatcactaa gattcatcaa tatcgagaaa ttatcggaat gcaagaactt
     1741 ttagatgaaa ataacgatag agaaaagtct gaatcataga caagcgtttg aagaggaaat
     1801 aaacacaaac aagaaggcgg atgagcgtta tttccgcttt tttgtttgta tttttctcgt
     1861 gaatacttat gaaatagtaa atttttatga aattctactc taagcatcag actttctttt
     1921 caaaatatgc tattttaaaa atgatgaaaa aagggggaga aaaatgaatt ttttagttgt
     1981 tttaaaagat actctgattg agcgatttac atttctatat gttcaattgc aggaaaataa
     2041 aacattgctg catttttcac cagagttcca tctagagggg ctaacattag gagaaatcta
     2101 tcaagcagaa gggctgtcgg ctgttttgca cttttttaag gtagaattgg aattaccagt
     2161 aaaagattat atcgagcttt ctcttgaatc ttgggatcga gcagtggttg aactgtttcc
     2221 agaaggttta atgatcgata caaacgatgg gaagatccat ctgactgcag cagaactgca
     2281 aaaacaaatg cgttatcaaa tagatgacga aaattctttt tctattttta gacgtcagca
     2341 aaagattcta aaaagctttt taaaagtgat cagtaaaaaa cggaatctga tcaaaacgac
     2401 acagctgttg aaaaaatatc caggtctgct tcacacgtct attcaatttc ctcaattgat
     2461 tacgatgatc catcgtttta tacgtatgca acagctacca tctaagaagc tgtttcttcc
     2521 attgacggac acatttcgag tagtcaacag agaagataaa aagaaagtga ttgtgattga
     2581 ctttaccaga aatcggaatg tccttcacca agttgcaatg gaaaaggcca attaaggctt
     2641 tttctttttg gatttttttg tcataataaa tattagatga tggaggagat aatatgatgc
     2701 aaacatggtt agaaaggcta atacgatttc agcagaacta tcacaaactt atgcagggaa
     2761 gatatgcgaa atttgatcag ttgaatcgcg gtctattgat cgctgcgctt gtctttagtt
     2821 tgctaagtcg ttggttgccc tatagaatcg gacaattgtt gttcatcgtc tttttcggat
     2881 ggatgatatt tcgtttccta tcaaaaaaaa tctaccccag actaaatgag aatcaacgtt
     2941 atttgaaata tcttgataga gtcaaagaaa aatatcatca gataaaaaac aaaaagcaag
     3001 tcaaaatgga tcgaaaaagt tatacttttt ttgagtgccc gaattgtcat caaaagcagc
     3061 gtgcacctaa aggaaaagga cgaatacgtg tgacatgcaa aacatgtggt ataaaattcg
     3121 aaacgaatgt ttaagggaga aaagaatgaa attagtatta ttacgtcatg gcgaaagcga
     3181 agcaaatttt gaaaattact ggacaggatg gctagatgtc gccttaacag aaaaaggaaa
     3241 agaacaagca gcagttgccg gtcaaaaaat gcttgaagcc ggacttcaat tcgatgccgt
     3301 ttatacctct gttttgaaac gtgcgattct taccggacaa gtcgcactag aagaaatggg
     3361 tcagttatgg cttcctataa taaaaacatg gcgtctaaat gaacggcatt atggggatct
     3421 tgtgggtaaa aacaaagata agatgaaaga aatatacgga aaagatcaag taaaaacatg
     3481 gcgcagagga tatacggaag ttccgccgtt aaccgcagaa aatagatttg accgcagata
     3541 tgatcacttg gatccgcatt tgatcccata cggagaaagt ctggaaatga cagtgaatcg
     3601 gatcattccc ttatggcaag atcatctagc accaaaacta aaagatcggc aagatttact
     3661 agttgtgggt catggtaata gcatccgagc actgacaaaa tatttagaag atgttcctga
     3721 agatcaaatg gatacgatcg atatcccaaa tgcacaacca atccagtata cattagcaga
     3781 tgatttgaca attctggaca aaaaaatact ttaaagaaca cctttatcta aaagtgagag
     3841 atagaaaaaa gaagcaaagg aatggaacaa aaggaacatg aaaaattata tatgggactt
     3901 tgatggtaca ttgtttgata cgtatcccgc aatggtagat ggtgcatggc aagcactaaa
     3961 agattttggt atcagtatgg acaaaaaaga aatctacttc aaaatgaaaa agtattctac
     4021 tagttatttg atcaatgaat caaacttgaa tgccagagag tttaatgaat tatttcaccg
     4081 atatgaaaaa gaaagtacgg aagtttcccg tccatttcca gaaacgaaac aagtactgga
     4141 aatgctaaaa gacaacggcg gtcgtcattt catcctgacc caccgtttga cagaatcaac
     4201 atggggcctg ttaaaagaac atcgattggc acatctgatt gaggaagtag tagggatcga
     4261 tcaggacttt cctcgtaaac ctgatccagc atctttaaat tatttgatcg atacttttca
     4321 tttagaaaga acagacacga tgatgatcgg cgatcgtcgt ttagatatcg aagcagggaa
     4381 aaatgcagga gtcgctactt gtctttacga catcgatcat tttctgggtg agatcccggc
     4441 agattatgtg gtaggaaatc tgaatgaaat ccttaccttg cagagcatca ttaaaaagta
     4501 gtatcccctt tcaaaggaga tatgttataa ttttcataaa ggagtgataa aattatatgg
     4561 aaatcagaag acaaaaagaa gttgtagaag catttcatta tgatatgaga ttgccgaacc
     4621 aagaagtaga aacagatttg aaagtaggct tctcgccttt aaaagaaacg atcgaaaact
     4681 atccgaaaga acatagcatc gtaggagcac gcttagagtt ccgtttagtg tttgatacat
     4741 atgttttatc tggaagtgtg agtcagatca atcatatcct caatcggaaa attgaaggtc
     4801 aaaatgatgt cacacaagaa gaagtggacg agctagtagc accgttattc gatatcgtac
     4861 gccgaatgtc ttatgaggtg acagagatcg ccttagatca gcctggggta aaattgaatt
     4921 ttcaatcaga aaaacaagaa tagacagtaa aaacatacct gcgatagatt aaagctatca
     4981 caggtatgtt tttttattac taattgtaag caaaaagaat gtcatttcga taaaaatcgt
     5041 ctaaaatata aaagtcactg aaaaaaattt tttcaggagg atggttgaag aatcattctc
     5101 gggaaccttt acttccttcg atggacttgt cattaataat atgaggagga gaaaaatgac
     5161 aacactcatc acagttttag tggttctagt ttttgcggca ttattatatg tgttctatcg
     5221 tatgcaaaag cgccatacaa aattttcaac acgcgtcttt acagcattag gagttggtat
     5281 cgtctttggc ggtctgcttc aattgatctt tggtgtagac gacaaagtcg ttactcaatc
     5341 aatggaatgg atcgggattg taggtcaagg atatgtatca ttcttgcaaa tgctagttat
     5401 gcctttagtg tttatttcga tcattggtgc attcacaaaa atgaaagttt ctgaaaagct
     5461 agggaaaatt agtgcgactg tattgacttc tttacttgga acaacagcta ttgcagcatt
     5521 gatcggaatt ggttcaacaa tgctgttcgg actccaagga gctaaattca cacaaggagc
     5581 tgcagaaaca gcacgaatct ctgaactggc aacacgtcag gaaacagttg aaaacttatc
     5641 gattccacag cagattttat cctttatccc taaaaacgtt tttgctgatt tagcaggtag
     5701 tcgtgcaaca agtacgattg gagtcgttat tttcgcggca tttgtcggaa tcgcttatct
     5761 aggcgtgaaa agaaaagcgc caaaagaagc agcattcttt tcagatttag tcgacagtct
     5821 gtataaaatc attatgcgga tcgtgacgct tgttttgcgt ttgacacctt atggagtatt
     5881 tgctttgatg acaaacgttt tggcaacaag tgattttgca gctatcgtca atttaggtaa
     5941 atttgtcgtt gcttcttacg tagcattgct tgctgttttg attgtccact cactgatttt
     6001 gattggtgca aaagtcaatc caatcaacta tttcaaaaaa gcctttccgg tattgagctt
     6061 tgcgtttact tcacgttcaa gtgctggagc tttgccattg aatatcgaga ctcagacaaa
     6121 agctttagga gtagatgatg caacagctaa tttctcagca agttttggtt tgtctattgg
     6181 acaaaacggc tgtgctggcg tttatccagc tatgttggca acgatcgtcg caccagcagc
     6241 tggagtgaat gtctttagtt tggaatttat tttgatgctt gtggcaattg taacaatcag
     6301 ttcttttggt gtagctggag tcggtggggg cgcaacattt gcttcactga tcgttttagg
     6361 ggcaatgaac ttgccagtag caatcgttgg gttagttatc tctgttgaac cattaatcga
     6421 tatggcacgt acagcagtaa acgtcagcgg tagcatggta gctggtatcg tgacaagtgc
     6481 acgcatcaaa gatttagatc gcgatgtcat caatgatgat tctaaagtga tcgatgtgaa
     6541 tgcatagtca aaaagaggtt gtgatagaag tggtcagctt taagaaataa gaagaagttt
     6601 cctaaaaata gtttctcaaa tttttgtgaa atttcggctt atttccgaga agctacttct
     6661 gaaacaccgt gaataagagg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc
     6721 aaacaaaaat agttcctcat attttacgtt tggttgagct tattaccgca gatgcaagct
     6781 tttgaacacc gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg
     6841 tgttagaaac atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag
     6901 aaaaaacaga tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact
     6961 tgaagatgct ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca
     7021 cccaaattcc ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa
     7081 gcggatatgg ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta
     7141 aagaagaaaa acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca
     7201 acaattttag aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac
     7261 tgatcaatcg agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag
     7321 ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg
     7381 ctattgatga aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag
     7441 taactgtccc agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc
     7501 ttatttactt tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta
     7561 ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg
     7621 ttttttcacg accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct
     7681 gaacaagagg agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc
     7741 tcaatgacaa ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac
     7801 gagtcgtgat cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg
     7861 taccgcgtaa atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa
     7921 ggaggaaaac agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt
     7981 tgaagctgga cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa
     8041 aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt
     8101 agggcatgct tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg
     8161 atatgatact ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt
     8221 agaagcgaag cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat
     8281 cgatcaagtg tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa
     8341 aatgggctta tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga
     8401 agcagtacgt aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata
     8461 tatcatcaac tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa
     8521 agatatcgaa ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt
     8581 agaaattgcg acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc
     8641 agaagacgag cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa
     8701 acaaatccca atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa
     8761 aatcactcca gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg
     8821 cgtaaatgtt atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat
     8881 ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat
     8941 taaaatcgat acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga
     9001 acctcggctg tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga
     9061 taaccaagaa acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt
     9121 acgttggatg gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca
     9181 aattcctgca tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc
     9241 ggatagtgaa aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt
     9301 gtggcctttc tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt
     9361 ccctactagc actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat
     9421 tttccaaagc ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt
     9481 gattcgtgac gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat
     9541 ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc
     9601 accaggacaa gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa
     9661 caaaatctgg aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga
     9721 gatcgattta tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga
     9781 aacgatcgaa cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca
     9841 actgtataac tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt
     9901 actttatgga gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact
     9961 tgatcagatc ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga
    10021 acatattcct catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga
    10081 gtttgatgat gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt
    10141 tcgaaatatt cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa
    10201 aacaaacgat gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt
    10261 ctgtaatccc gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc
    10321 agctgttctg acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga
    10381 agaaatcaaa cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca
    10441 aggaaaactt tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga
    10501 gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag
    10561 tttgcgtact attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa
    10621 aagctagctg attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt
    10681 cataaggaga tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta
    10741 ggagaaggag ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc
    10801 ctctctctgt ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta
    10861 taataaaaga gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca
    10921 tagtcgatta ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt
    10981 ggtagggcat ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg
    11041 ttctactgta gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt
    11101 tacttcacct tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc
    11161 agacaatgat ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca
    11221 atttgaagca gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta
    11281 ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga
    11341 ttctaccaat gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac
    11401 cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca
    11461 aggcgttcca gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac
    11521 agctaaagca aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct
    11581 gcatcctgat gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc
    11641 tcaattaacc actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga
    11701 attgtatttt gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa
    11761 gggattgaag aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt
    11821 tgttttagat ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa
    11881 agaattcaaa ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag
    11941 agaaatgcta gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga
    12001 acatccaaaa gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat
    12061 cgtttcattg tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt
    12121 attagttgtg accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt
    12181 aggaggagat catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca
    12241 cagagttagt gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg
    12301 ataaagacct gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc
    12361 atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc
    12421 aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac
    12481 tcctctcttt tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag
    12541 cagtcattga acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt
    12601 ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga
    12661 agctaggtac agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag
    12721 cagcttctgc ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag
    12781 aattgatcaa aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa
    12841 aataaaaatc catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt
    12901 tgggaaaacg attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa
    12961 tttatggcgc ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc
    13021 aacaccctta tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg
    13081 cctctttacg acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga
    13141 aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa
    13201 gagcggctcc accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga
    13261 aagatcacaa acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct
    13321 taaaaaaaac agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt
    13381 tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg
    13441 gtaatgttat tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag
    13501 aaatgatggg gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt
    13561 tgagagaaga aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca
    13621 agtgtacaat tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc
    13681 tatcgtacac gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat
    13741 tatggaaaac ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg
    13801 tgaagatgga agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac
    13861 attagaagaa ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc
    13921 tgcaaacgtt gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt
    13981 ttacgagaga acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg
    14041 caggtatttt tttgtatgaa aaagtaaata aatttaagag gctatgaaag agccaccttg
    14101 tatcaaggaa taagaacaaa caaaaatggc ttctcatgtt tttcatttgt tttctcttat
    14161 attcaggaca aggtgctgat ttcataacct cttatttatt cttcatctgg tgtcattacc
    14221 attggcaaaa tcattggatg tctttctgta cgttcgaata agaaaggctg aagcgcgcca
    14281 gtcatcgctt ctttcaattt atattctgaa cagtcaggtc cattcaaaca ttctcttaaa
    14341 gcgtggaata agattcgctg agcttcattg atcatttctc ctgattctcg catgtagaca
    14401 aatccacgag agagaatatc tggaccagcc aagatttctt ttttcttgat atccactgtc
    14461 gcgacagcta atacaagacc ttcttctgaa agcaggtgac ggtcacgtaa aacgacatta
    14521 ccaatatcac cgatcccgct accatcgata tacacatctc cagccacaaa atgcccagca
    14581 ggacgcgcag aatcgctagt taacgctaaa acatcgccat tttccatgat gaagcagttt
    14641 tctgcaggaa cacctacatc ttgtgctaga gaagcatgta ctttcaacat gcggaattct
    14701 ccgtggacag gaataaaata ttttggcttc atcaaacgaa gcattaattt ttgttcttct
    14761 tgtccaccgt gaccggaagt atggatgtta ttgatttttc cgtgaatcac ttctgcacca
    14821 gcttcagaca gcaaattgat caaatgattg acacttgttg tattaccagg aataggtgaa
    14881 cttgaaaaga tcactgtatc gtctggctgg atttggattt gacggtgagt accattcgcg
    14941 atgcggctca acgcggccat cgcttctcct tgagaacctg tacataggat catcgtttca
    15001 ttggcaggca agcgattgat ttcgtgtgca tctacaaatg tgccgtctgg aactttgata
    15061 tatcctaaac gttgaccgtt gacgatggca ttttccatgc ttcgtccgaa aacagcgatt
    15121 ttacgaccag ttgccacagc ggcatctgct gcttgctgca aacggaagat gttagaagca
    15181 aagctggcaa aaatgatacg tccttcgatt tttgaaaaga tcttcaaaat agaatgccca
    15241 atcgtttttt ctgatttagt aaatgttgga atctcagcat ttgtactatc agataaaagg
    15301 cacaagactc cttcttcccc gatacgagcc attttatgca agtttgctgg ttcgccaaca
    15361 ggagtgaaat cgaatttgaa gtctccagtc gctacgatat ttccagaagg tgttttcacc
    15421 acgatcccta atgcatctgg gatactatgg gtcgttctga aaaagctgac agaagtcttt
    15481 ctaaaacgga tgatcgtatc ttcattgatc tcatggagct ctgcatctcg aagcaagcca
    15541 tgctcatcta atttgtttgt gatcaacgct aatgcgagag gaccagcata gataggaata
    15601 tttgcttgtt tcagcaagta gggtattccg ccgatgtggt cttcgtgccc gtgagtaatc
    15661 accaaggctt ttactttgtg taagttttgt atgatgtagc tgtagtcggg aatgacatag
    15721 tcaattccaa gtaaatcgtc ttccgggaat ttgatcccgg catcaatcaa aatgatttca
    15781 tcttggaatt gtaccccata cgtatttttc ccgatttcac ctaatccacc aatagcaaag
    15841 acgcctgtct cgttattttt gatggaaagt ttcatattaa aactccacta atgagaaatc
    15901 agcattttct ttttcgtact ctagatgact tccttctaga agttgaacga attcaatatt
    15961 gtatggggta ttttcttcca cacgttggcg agcgtctaca tcgctttttg cctcaatgta
    16021 aagagatttt gtttcttccc gtttagggtt gcgtactttt gtttcttgat aataaacttt
    16081 atagatcatt gttttgtctc ctttatctgc gtaaagcagt gatttagcta gttgattttt
    16141 ttagacaaca caaaacgagg ggacaagcca cctggttttt catatgcttt gactagaata
    16201 ctggattcga gtcaaagaga caagcctcgt ttttgcttat ctgttcggat attttttcaa
    16261 ccgtaccttc tgtataaaaa atatacaaca attaattgtt attctagcat aaaacagcaa
    16321 ataagtatag aaatcaacta gattgaaaga aaaaatcaat gtttatcatc ccgctataat
    16381 cataatgact tttttatgag agaagtgttc tattaatagg ctcgttgtgc tatcattttt
    16441 agtaatgaaa atgtaacaaa agattaaaag gagatgagaa agtgaaacta atgtggcatt
    16501 ataccatgcg ttataagaaa ttcttgtttt tcaatttcat atgcgtattt ggttttattt
    16561 tgattgaatt aggacttcct acgattttag cacgtatgat cgatgtagga atccgcaatg
    16621 atgattttga ttatgtgaag caacaaggga tatttatgat cgttattact gtaatcggta
    16681 ttattttgaa tatcttgtta ggttattttg gttcgaggat tacgactaat atcgtagcag
    16741 atattcgaga tgatttgttc gaaaagatcc aaagttattc gcatcaggaa tacgaaaatc
    16801 taggcgtatc ttcattgatc actcgtacaa caaacgatgc ttatcagatc atgttgttcc
    16861 tgcaaaatat tttacgtatc ggttttatgg caccaatgat gttcattgtc agtttgtaca
    16921 tggtcatgcg tacaagtgtg acactcagtt tgtatgtagt aggtgcatta cctttactat
    16981 tattggcagt cgtagcgatt gctaaggtgt ctgaaccgtt atctaaaaag caacaaaaaa
    17041 acttagataa aatcaacagt atcttgcggg aaaatctttc aggattgcgt gtgattcgtg
    17101 cattcgtcaa tgaaaagttt gaagaaaaac gttttgctgt agtcaatgag aattatgctt
    17161 ctagttcaaa aagcttattc cgtttgatgg ctgttgcaca accgggtttt tatttcttat
    17221 ttaatatcgt aatggtattg atcatctgga gcggggcagt gcaaatcgat gcaggcgagc
    17281 ttcgtgtagg agatttgatt gcgtttatcg agtatatatt ccatgccttg ttctcattca
    17341 tgttatttgc tagtgtgttc atgatgtatc caagagcagc tgtttcagct ggacgtatcc
    17401 aagaagccct agatgccaaa ccttcgattg tagaagatcc aaatggtata tctgatacaa
    17461 aaactaaagg gtatatcgaa tttcaaaatg taacatttgc ttatccaggc cattcgcaag
    17521 agccagtgat acgaaatgtc agttttacag cctctccagg agagaccgtt gcttttattg
    17581 gaagtaccgg tagcggaaaa tctacgttga tccaattgat ccctcgcttt tatgatgtga
    17641 gtgaaggaaa gatcttgatc gatggagtag acgtgagaga ttatcggcta agcaagttgc
    17701 gagataagat tgggtttatt ccgcaaaaag cgctgctatt tactggaacg attgcagata
    17761 atttgcgata cgggaaagaa gatgctacgc aagaagaaat ggaacgtgca gctgagattg
    17821 ctcaagcttc agaattcatt tctcgaaaac cagatggatt taatgaacat ttatcagaag
    17881 gtggttccaa tttttcaggt ggacaaaaac aacgattggc aatagccaga gccatcatac
    17941 gccgtccgga aatctatatt tttgatgaca gcttttctgc attagattat caaacggatg
    18001 cgaagttacg tgctcgtctg aaaaaagaaa cgacagaatc tactgtattg atcgtagcac
    18061 aacgtgtggg aacgatcatg catgcagata agatcatcgt attaaatgaa ggagaagtcg
    18121 tcggtatggg gacgcatcgg gagttgttgg aaaaatgccc gatttattat gatattgccg
    18181 cttctcaatt atcaaaggag gaattagcat gaaaaaaacc ttttcttcct taaaacgttt
    18241 agctcgatat atcagaccgt atcgtgagac ttttctttta gtgatcctat ttacagttct
    18301 gacagtggct ttcaacgtgg ctttgccata tgttacagga cttcctacta cagaaatcag
    18361 ccgtaatatt gccaatggcg aatcaatcaa tttttcttat atattcaaat gtctcatttg
    18421 gatcacagtt gttggaatag ggtattgtat ttcgcaatta ctttctggtg ttttgatgac
    18481 aaatgtcgtt caaagtgcca tgcatgattt gagaagagac atagaagaaa aaatcaatcg
    18541 tcttccggtt tcatatttcg ataaaaacca gcaaggaaat attttgtctc gtgtgacgaa
    18601 tgatgtagat gcagtcagcg gtgctttaca acaagctttt attggtatcg taaatgcgat
    18661 attgggaatt acgttggctg cagcaatgat gttttatatc cagccgatga tggcgctgat
    18721 ctctatgatc atgattccaa catcgatctg gatctcaaaa cgagtgatca atgcttcgca
    18781 gaaatatttt caaagcatgc agaattctct tggtgagcta aacggctacg tccaagaaaa
    18841 tatgacaggt tttagtgtat tgaaagtata tggacgcgaa aaagaaacat tggaaggatt
    18901 caaaaaagtt aatcacagct tgaaatactt tgggtttcgt gcagcattca tttctggctt
    18961 gatgatgcct ctagttcaat taactgccta tgctacttat attggtatgg ctgttctagg
    19021 aagcttttat gttattactg gggcgatcgt tgtcggtcaa ctacaagcat tcatccaata
    19081 tatttggcaa atcagtcagc caatggggaa cgtgacacaa ttgtcttcag ttttgcaaag
    19141 tgcatcagcg gcaacgaaac gtgtattcga gattttagat gagccagaag aaaaagaaaa
    19201 caaagtagat gcttcactgc cagaaacgat tttaggagat gtgacattcg atcatgtcga
    19261 ttttgcatat gatccgaaga aaccattaat ccaagatttg aattttgaag tgaaagctgg
    19321 tcaaaccgta gctgtggtcg gtccgaccgg tgccggcaag acgacattga tcaatctatt
    19381 gatgcgtttt tatgacgtag acaaaggagc aataaggatt gatggaatcg acaccaaatc
    19441 aatgagtcgc agtgatgtgc gttcgctatt tggtatggtc ttgcaagatg catggcttta
    19501 tcaaggaacg attgcagata atatccgctt cggaaagctt gatgcgacag attatgaagt
    19561 agttgatgct gcgaaaacag caaacgtaga tcattttatc cgaacgatgc cagatggata
    19621 tgatatggcc atcaattctg agggagacaa tgtgtcttta ggacaaaaac agttgctgac
    19681 gattgcccga gcagtggtct ccgatcctaa aatcttgatt ttagatgaag caacaagttc
    19741 agtagacacc cgtttagaag cgttgattca aaaagcaatg gatcgcgtga tggagggacg
    19801 gacaagtttc gtcatcgctc accgtttatc gacaatccgt gatgcagatc tgattctagt
    19861 gatggatcaa ggtcagatca ttgaaaaagg aacgcatgag agtttattgg cacaaggtgg
    19921 attttatgaa aaactgtata acagccaatt tgctgaggaa gcagaataag tcatataaga
    19981 ataaaacagt ttaaaactta ttttttgttc tctattcttg ataatggtaa agtaaagtta
    20041 tcaagaaaag tgaggacacg gagatggaac agtttttaaa tgaaaaaatt actgaagcca
    20101 aaagagaagt tgatcgttta acgcacgtct gtcagacgaa gccttttgat tcagctgatt
    20161 tttttgaaaa tgagtttcaa atcgaacgct tggcagcaca aatagaagtg tacgaattaa
    20221 ttttaaaaaa agtaatagaa gaaaatatag atgtacaata aaaacaggta aaacgtcagt
    20281 taccagatac tgtaaataga catctggcct gagcaggttt tacctgtttt ttttcttatg
    20341 aaataaatat atgctatgat acctttattc aataggatta gtttctacaa aaaatgaaag
    20401 gagaaaaata aatatggaac ggtttatagc atcaaatgcc acagtgattg gtgatgtgac
    20461 attgtcagaa gatgtgacga tctggtatca agctgtttta cgcggggaca gtaattggat
    20521 aaaaatagga caaagaacga atatccaaga tggtacaatc atccatgtcg atcatgatgc
    20581 acctgtagat attgcggaga atgtgacggt aggacatcag tgcatgctac atggctgtac
    20641 aatcgaaaaa ggggcgctga tcgggatggg gaccacgata ttgaatcatg cggttattgg
    20701 tgagaatagt ttgattggtg ctggatcatt agtaacagaa ggaaaagtta ttcctcctaa
    20761 tgtcttggct tttggtcgcc cagctaaagt gatccgtcct ttgacaaaag aagaaataca
    20821 aaaaaacaaa gaaaacatcc agcattatgt cgagataggt cggatgcatg cgcaaggaaa
    20881 attcaaggag caaagatagg cagtagcatc cataaaataa aaaaggatgg tcagtttgag
    20941 ttgaccatcc ttttttattt ggtttagata agagagctta aaaaagcttc ctttgaaaca
    21001 gattaattta atgaagaact gttttccatg acttcatcga tcaatccata tgcttttgct
    21061 tgttcagctg tcatatagtt gtcacgatct gtatcttttt caatcacttc aagcggttgt
    21121 ccagtgcgtt cagctaaaat tttgtttaag cgttcgcgtg tttgcaaaat atggcgtgcc
    21181 gcaatttcga tttcagttgc ttgcccttga gcgccgccaa gaggttgatg gatcatgatc
    21241 tcagcatttg gtaaagcaaa tcgtttacct tttgtaccag ctgtcagcaa gaagcttccc
    21301 attgaagcag ccatacccat aacaattgtt tggacatctg ctttgacaaa gttcattgtg
    21361 tcgtaaatag ccatacctgc tgtaactgaa ccaccaggag aattgatata aagatagata
    21421 tctttttcag aatcttgagc gtctaagaaa agcaattggg cgatgatcga attagcaaga
    21481 tcatctgtta cttggccgct taacatgatg atacggtctt tcaataaacg tgagtagata
    21541 tcgtaagctc tttcacctcg tgaagattgt tcaatgactg taggaattaa attcataata
    21601 gagttcctcc ttgatttcgt ttagaaatag tgtaccataa tttagtttct aatacctaaa
    21661 atgtagtata catttttggt caaaaaaggt caaataaaaa acttaggcaa attagtcgca
    21721 taaacccgct ttatggtatg ataagcagag aaacaaaaca aggaggaaca acagattgat
    21781 agttggtatt actggcctta tcggagtgct gacaggtgct gtagttgtct atatcggcat
    21841 gatactgcaa atgcgtcact tggaaaataa agaacagcgt agacgtgaat tggaattgaa
    21901 agtcaaagaa atcgagacgc tgaatcaatt aaataaaaag atcaacgaga tcttgcaaaa
    21961 acggacaacg ctgatggaag aatatgtcag ctttgatgca tttgatgatt gttatatcac
    22021 gattgacgat tttgcgtatc tccaatcatt ttcagcgcaa aatagttttt atctgccaaa
    22081 ttatatttta gacgagctat ttaagaatat ctcccatcga aaagtgattt tatctccaga
    22141 tgaaacaatg aagattggtg gatatgctta taaaggcgga cgcgtgattt tagagaattt
    22201 tacggatagt ctgactgaaa tgatcaatga aaaaaaagct cagttgaaga ctatgacgcg
    22261 ccagccgatt ggtttcttct cgaaagatga tgtttggagc tgatattttg aagcattgcc
    22321 gttgcacatg agagcgtggt aatgctttct ttttttctta atgatacaat agaactgaac
    22381 ggagtgaaga aagatgttac aaatctatgc ccatcgcgga agcagcggca cccacccaga
    22441 aaatacgctt cctgcatttg cagaagctgt acgagtaggt gcagatggta tcgaactaga
    22501 tgtccatcta agcaaagatg gttatttgat cgttatgcac gacgaagaag tggaccggac
    22561 gactaacggt aaagggctga ttcgagaaaa aacattggaa gaactcaaaa aattgaatgc
    22621 agggggcaaa ttcagcaaag aatttccagc agctaaagtt cctgaattga gagaagtgtt
    22681 aggactgctc ctccaaaaaa attaccgagg gatgctaatg attgaaatta aaactgatca
    22741 gtatgaatat cccggtatcg aagaaaaatt agccgaaaca ttaaaaagta gagattggcc
    22801 ttttcaacat gcatattgca gctttcattt ggattctttg gaaaaaatcg cattactgga
    22861 accggagata cagctggact tattgatgag tacttctgat aaaaagatcc aactagcagc
    22921 agaaacccct tatatagaag gagtacatcc aaaaaatact tggttgttcg aacatccagt
    22981 ccaggcagct gattttccaa aagcaattcg tgcatggacg attaacgatg aagaagaaat
    23041 ggccaaatgt caagaactca aaatagatgg aataataact gattttccgg agaaagctct
    23101 tcggtggaaa caatcaacga aaagtaggta aaatatggaa aaagtattag tgatcgtcgg
    23161 tccgacagca gtcggtaaaa cagcgctaag tgtggaactc gcacaactat ttggaggaga
    23221 gatcatcagt ggtgattcat tgcaaatcta taaaaatctg gatatcggta cagcaaaaat
    23281 caaagaagaa gagatgaata atgttccgca tcatttgatt gatgtgatcg aacctactca
    23341 acgttactca gccgctgatt ttcagcattc aggtcgacaa ttgattaccg agattgcgaa
    23401 ccgagggcac ctacctatca tagctggtgg tacgggtctc tatatccaat cccttctata
    23461 tgattatcag ttaggagcga cagaagaagc gaatcctgag attagaaaaa agtatgaaac
    23521 gttagctgaa acaattggtt cccaagcatt atggaatcag ttgaaagaaa aagacccggc
    23581 agctgctgag aaaatccact ggaacaatca acgcaaagtg ataagagctc tggaagtttt
    23641 agagacgaca ggacatagta tcacggcacc taaagaagag ccaaagcaat tgtatgatta
    23701 cctcatgatt ggattagata cgaagcgtag tattctttat gaacgaatca atcaaagagt
    23761 cgatttgatg ctagcagaag gtttagtagc agaagcgcgt aaggtgtatg agcttggaga
    23821 agtccaggcc agtcaaggta ttggatataa agaatttttc ccttattttg aagggaaaga
    23881 aacattggaa gaagcaacag aacaagtcaa actacattcg agaagatacg cgaaacgtca
    23941 gttgacgtgg tttcgtaacc gtttggatgc acagtggttt gatcttataa gtgagccaaa
    24001 tcaaaaaagt cagattgagc aaacaatcaa aaaatggctg gaggcggaat aaaatggaag
    24061 aaaaagtgat catcgtaggg gtagaaaccg aagcaaacca gcgttacttt tcagagtcaa
    24121 tggaagagct agttcagctg acgaataccg catcaggaga agtcgtattc acaattacac
    24181 aaaagagacc acaagtggat cggcaaacga ttatcggtaa agggaaatta caagaattag
    24241 ttcaacaagc ggatgcgcat gaagcggatt tgatcatctt taatcatgag ctaactccga
    24301 gacagagcca actgattacc gatgctgttg gtcttccggt aattgatcgc gtgcagctta
    24361 tcttagatat ttttgcgatg cgtgctcgtt cgaaagaagg gaagctgcag gttgaacttg
    24421 ctcagttaga atacctgctg cctcgtttag tagggcaagg gaaaacatta tctcgtcttg
    24481 gcggtgggat cggaacaaga gggccaggtg aaacaaagtt agaaacagac cgccgacata
    24541 tacggaataa aatttttgct gttaagaaag aattgaaaga agtagaagct catcgagagc
    24601 gcaatcgcca aaaaagaaaa aatagcgaga tttttcagat tggacttatt ggctacacga
    24661 atgccgggaa gtcaactatc ttgaatctat tgacacaagc ggatacttat tcgaaagatc
    24721 aattgtttgc aacgttagat ccattgacta agagatggcg ttttgctgaa ggatttgaga
    24781 ttactgtaac ggataccgtc gggttcatcc aagacctgcc tacgcaactg atcgatgctt
    24841 tccattctac attagaagaa agtcaaaata tggatctttt gctacatgta gtcgatgcta
    24901 gttctccaga tcgtatttta caggagcaga ctgtcttaaa attgatggat gaactgaata
    24961 tgaaagaaat gccgatactg actgtttaca ataaggcaga ccagatcgac ccagcgatgt
    25021 tcacgcctac gctattccca aatgtattga tttctactca gacgaaagaa ggtaagatgg
    25081 cgttgatcga agcaatcaaa cgtcagctga tggaactaat gaccccttat acactttctg
    25141 tgccaagcaa cgaaggacag aaattaagcg agttgagaag acaaacaatg gttttggacg
    25201 aagcgtacat ggaagaaaca aacgaatatg aagttaaagg gtttgcaaaa accaattcaa
    25261 aatggttaag aaaaaaccaa taaaatttta tttttttaa
//
LOCUS       DACJGB010000040        25242 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.040, whole genome shotgun sequence.
ACCESSION   DACJGB010000040 DACJGB010000000
VERSION     DACJGB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25242
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.040"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(51..1598)
                     /locus_tag="IYI34_001687"
     CDS             complement(51..1598)
                     /locus_tag="IYI34_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAR0559557.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IYI34_001688"
     CDS             complement(1700..2053)
                     /gene="tnpB"
                     /locus_tag="IYI34_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAR0559558.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(2043..2237)
                     /locus_tag="IYI34_001689"
     CDS             complement(2043..2237)
                     /locus_tag="IYI34_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559559.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(2481..2765)
                     /locus_tag="IYI34_001690"
     CDS             complement(2481..2765)
                     /locus_tag="IYI34_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559560.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(2783..3673)
                     /locus_tag="IYI34_001691"
     CDS             complement(2783..3673)
                     /locus_tag="IYI34_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAR0559561.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(3693..3911)
                     /locus_tag="IYI34_001692"
     CDS             complement(3693..3911)
                     /locus_tag="IYI34_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559562.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(3881..5581)
                     /locus_tag="IYI34_001693"
     CDS             complement(3881..5581)
                     /locus_tag="IYI34_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAR0559563.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            5848..6621
                     /locus_tag="IYI34_001694"
     CDS             5848..6621
                     /locus_tag="IYI34_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559564.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(6702..7271)
                     /gene="thiT"
                     /locus_tag="IYI34_001695"
     CDS             complement(6702..7271)
                     /gene="thiT"
                     /locus_tag="IYI34_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAR0559565.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(7337..7439)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7445..8416)
                     /locus_tag="IYI34_001696"
     CDS             complement(7445..8416)
                     /locus_tag="IYI34_001696"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAR0559566.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(8510..9883)
                     /gene="glmU"
                     /locus_tag="IYI34_001697"
     CDS             complement(8510..9883)
                     /gene="glmU"
                     /locus_tag="IYI34_001697"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAR0559567.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(10386..11204)
                     /gene="purR"
                     /locus_tag="IYI34_001698"
     CDS             complement(10386..11204)
                     /gene="purR"
                     /locus_tag="IYI34_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAR0559568.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(11371..12195)
                     /locus_tag="IYI34_001699"
     CDS             complement(11371..12195)
                     /locus_tag="IYI34_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAR0559569.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(12168..12848)
                     /locus_tag="IYI34_001700"
     CDS             complement(12168..12848)
                     /locus_tag="IYI34_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAR0559570.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(12871..13809)
                     /locus_tag="IYI34_001701"
     CDS             complement(12871..13809)
                     /locus_tag="IYI34_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAR0559571.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(14054..14905)
                     /locus_tag="IYI34_001702"
     CDS             complement(14054..14905)
                     /locus_tag="IYI34_001702"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAR0559572.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(15059..17632)
                     /gene="mprF"
                     /locus_tag="IYI34_001703"
     CDS             complement(15059..17632)
                     /gene="mprF"
                     /locus_tag="IYI34_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAR0559573.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            17773..18579
                     /gene="proC"
                     /locus_tag="IYI34_001704"
     CDS             17773..18579
                     /gene="proC"
                     /locus_tag="IYI34_001704"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAR0559574.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(18658..20214)
                     /gene="rny"
                     /locus_tag="IYI34_001705"
     CDS             complement(18658..20214)
                     /gene="rny"
                     /locus_tag="IYI34_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAR0559575.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(20625..21674)
                     /gene="recA"
                     /locus_tag="IYI34_001706"
     CDS             complement(20625..21674)
                     /gene="recA"
                     /locus_tag="IYI34_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAR0559576.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(21783..23024)
                     /locus_tag="IYI34_001707"
     CDS             complement(21783..23024)
                     /locus_tag="IYI34_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAR0559577.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(23264..23842)
                     /gene="pgsA"
                     /locus_tag="IYI34_001708"
     CDS             complement(23264..23842)
                     /gene="pgsA"
                     /locus_tag="IYI34_001708"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAR0559578.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(24051..24935)
                     /locus_tag="IYI34_001709"
     CDS             complement(24051..24935)
                     /locus_tag="IYI34_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559579.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         7170 a         5472 c         4385 g         8215 t
ORIGIN      
        1 tacgtcaaat aacccgacct atagaataga atcgggaatg tttggcgttt ttagttacga
       61 gaaaaatgtt gttgtacatc cttgttccaa ggcaacagtt gatctaaagt ttctgctgaa
      121 agtacgggaa gatttggtat cttatcaaat agaaactcta gatactttac aggattcaat
      181 ccgttaagtt ctgctgtttt atatacactc agaatgattc ctgaagttcg tgctccttca
      241 agacttgtgg aatgcaacca atttttccgt cctatcacca gctctttgat atttcgctcc
      301 gcacgattat ttgtgagttc taaacgacca tcttcaaaaa taggttcaaa accacttcgt
      361 agtttacacg catattgaac ggctgcatcc aacttggatt ttgcaacagg atctatcgtt
      421 gccatccact cataaaaagc aataaataat ggacgcagtt cttgttgccg acagcttaat
      481 cgcttctcag gagaaaagtc tttccatttc ttttctagga caaaccattt atccaattgt
      541 ttgactgctt gagctgcggg aatatccgtg tttttctttc cttttggaat tgcttcgaaa
      601 aatttccgcc gaacatgcgc gaaacaatag acaagtcgta cactttccac ggcattatac
      661 ccgctatagc cgtcacagtg taaataccct gtaaactcct tgagaaaagt tttcggaaca
      721 tctccagcac gactttcgtc gtggtgataa taaacgatgg gatgctgact gtcttttcct
      781 gtacaaaact gccagtaata ggtatctgtt ttggcacttt caatcacacg ataacttgtt
      841 tcatccccgt gtagaatctc ttcttgatta agatagactc ttaatcgttc tcctaaaggg
      901 tctaacgcgt tttgcactgc tttaatatgc caattggtga tattatctct cgaaatatcc
      961 aacccatgtg tttgtttcca atagttctct tgccgatagg caggaacttt ctgaccaaat
     1021 ttcatccgga tcgtttccgc cactaaacta ttggaaccta agctattaga aatcaaaggt
     1081 ttaggaacga tcgctttttt aaaagaagaa agtccttctt tttcgcagct tttacaacaa
     1141 taggtatgtt gataatgggt gagacaatcc aacatagcct gatggaaacg aatttcttct
     1201 cgtacttttg tccgtccgat atctttcatc tgtgaaccgc aatgttcaca aaattgttct
     1261 tccaaaggaa ggagatgttc tttctctgtt ataggtaagt gtttgatttt gtctgctttc
     1321 gttcctacaa ctcgttgatg tccttttact ggaataatgg tggcagctgc ttctggaaca
     1381 cttacagaaa tttcttcatc actaaaaaga ttcaattgcc cattcgtttc ctcaaagaga
     1441 gtctctttag agcgtccaaa taatttgttg gttaaataat tgaccttttc attcaatagt
     1501 tggatttcag ttgtaagatt ctgaatggtt tgttgttgtg cttgattggt tttatgggct
     1561 tcttgaagca actgaagcaa cgtatctgta gtttccaaaa tttttcacca ccttgaatcg
     1621 aactgatttt gttacagaat accacaagaa acgcacaaga aataggggtt caacacattt
     1681 tttgtgttaa ccccccagct taaaaaactc ctttttttcc tggcaaaata gctcgaggtt
     1741 gatcgatact taatccttct aacaaccatc gaacctgttg gttcgttaag gttcgaactt
     1801 cgtctttcgt ccgaggccat tttaacttgc cgttttctat ccgcttataa cagagaagaa
     1861 aaccatcacc gtcccaatag agaagtttga aacggtcttg acggttcccg caaaataaaa
     1921 aaacggcatc ttcgtaaagc tcgagttgat actcttgttg aataagactg gcaagaccat
     1981 caattccttt tcgcaaatct gtttttccgc acacaatata aatatgtttg gtttgaagta
     2041 aatcataaag cattggacaa cagttctttc aaaatagctt gaagaatata attgttaata
     2101 ccgttataga tcaaaacttc agaaccattc tctcttttga tgcgcatcgc taacttggca
     2161 gccgcgggtt tacctttttc tggtgcactc ttttcgtttc ctaattggac aggaacaatc
     2221 tcatttaatt tatacataga aaacctccag acttcttgat agctcaagta tatcttgagg
     2281 tgcatttgtt agtaaggtac tgttctatgg ggcgcttact taataccgag aaaatggttt
     2341 ttgaacatca cttattcaaa ataaaaaaag attgtgacac gaattttgtc acaaccttta
     2401 aatgtattcc tagagcaaag aagctaagcg aagaaatcca attctgatta tttattgtat
     2461 ttctttgttc agcatccttg ctaaaagccc atattggaca aagtattcac tacacccatg
     2521 atctcatctt ttcgagaacg tgctttttct gtcggatctt ctacctcttc tgtgtcaatt
     2581 cgaacaataa atttccgatt catcgtcacg taagcaccgt cgttgatcaa aataaacagt
     2641 tcgttctcat tatagtctgc acaggcatct ttccatacgt caaagccgtt tttttcagaa
     2701 gggatatccc gtttaaccac agtttttcca cttaattcgt ggaacgttac gtcataatat
     2761 ttcattatct ctacctcttt cgtcattcaa tattttgtac ttgttctctg attttttcta
     2821 agatcgtctt agactggacg acttgatttt taatttcgat tgcatttgat ttggaaccga
     2881 tcgtgttgat ttcccgattc atttcttgga tcaggaaatc taattcccga cctacaggac
     2941 attgtgcttc gataagttca tctagttttt gtacatggat cgccagacga tccagttcct
     3001 cgtgaatatc tccgcgttcc aacaagatgg ctagctccgt caacaaccgt tgctcatcga
     3061 ctttcgctcc aagaaattcc tccagctttt tttgatacct tatttgaaat tccttttcat
     3121 agatttctac aaaactatcg agttttttta gtacttcttt gaattcttca gtattctttt
     3181 tcaacactaa agctaaagct tgaccttctt tttctcgact cgcctcaata gaatcaagtg
     3241 cgcttttcaa tgtatctaga gctaactgtg ccaattcttc catgctctct tccggttttt
     3301 cttctaccgt tacgaagttt tcgtccagcg cgaagcgttc cgccatccga ctaattgaca
     3361 cttgattttg actaccgtat cgttgttgaa ttccgtctgt caattgagtg ataagtgaat
     3421 caatcaattc ccagttgatg acgatctgtt tttcgttttt gccaatataa gctagattca
     3481 caaatacttc gattcgaccg cggcttaact gctgtttaat aagctgacga aaatcgtttt
     3541 caacaaagtt caggatttta ggacttcgta tttgaacatc tagaaaacgc tggttgacac
     3601 tttttatttc aatatctaac tgatacgctt ttgtttcgcg gatggctttg ccaaaaccag
     3661 tcatactttt cattcgacag ggtccttcct cattacatat tttctattgc tttttgcagt
     3721 tgttcaaact cttcgttcgt caatgtcagg cctttcccca ttttttcatg gtctggcgcc
     3781 caatctcgaa gatcgaattt tggcgggcga ccattccagc tgactaggtt cagttcttta
     3841 cgccacccct tgttgttttc tgacaatata gcgatttctt ctacgatttc atatgaaaat
     3901 tcttgtgcca tggttgttcc tccttgagat aatataaata taggatcagt aatgtgtcaa
     3961 ctggttgctt aagcaattca tgctgcaaac gatccttgcg ttttccttgt agcagataat
     4021 caaaataaac agttttcgtg cgctcatttt gtattttttg ctggtatttt cgataccttc
     4081 gccattcttc agtacttaat tgaccaattt gtgccaataa atcatccggc atacttttta
     4141 tatcttgttg gcagatcatt tgaatcatca tgtctacttg atagatttca ttgggcagta
     4201 aaaaatgttt ttttgctaaa agttctagca tcgccaatcc gcatgtctct ggtacgaccc
     4261 tgttcttata taaattacgc agtttttgcc aaaattttat gcttttgaaa aaagataatt
     4321 ctaactgaat ttcttttgtt aagtaagaca aaaatcctcg ccagtatgtt cttgcaggtt
     4381 taacagaatg aaatgtatac cagttcccat agtaatagcc taatcgtttt ttcatttcca
     4441 gatagcctaa ttgtaaattt aattggttcc tttggctatc aaaaagcaga aagctgccta
     4501 gtgtccacaa tgtttgacat ttccaatgga taaaaatgtc tggcatacga atttttttcg
     4561 ctgggcctgg cccttgcaca tctacgacaa atgcctctgt tgctcctttg ttaatcgcaa
     4621 tatcaatagg tattttgttg cgatacccac cgtctgcata tttatttttt ccgatctttc
     4681 gataagccat agcgggataa aatgaagcag aggcgagtat ccaatctggt atttcttctg
     4741 tttttgtctg ttggatatca gtcactactt ccgctagttc tggcagttta gtagacaccg
     4801 tatataaacg tattttggga ttatgtttga gtaaggacaa atccaaattg tctttgacca
     4861 tcttttccaa tggagcagaa gaaaccccgc cttcaattag tgctgttttc gtcatttggc
     4921 gtctttctcg atagaagcgt tcacgaaggt cttcaactgc agccatttct ggtaacgcaa
     4981 gcacttgtct tgtcgacagc tttttccata aattcacagc ttttttcata tctccttgta
     5041 aaatcaatgc gccgttcaac gcacctaccg aggtacctgt gataatctca aagttgatat
     5101 catgttcctt cagcgcctgc cacacaccaa tctgataagc accgtgcgct cctcctccac
     5161 ctaaaacaag tgctgtatga tagaccagct ttttatttaa ttgtgtggtt ccttgatagc
     5221 cgtttttatg aaaaatacgc aaccattctt ctgatagagg agattgaaat aaaaaacaac
     5281 agttttcttt aataaaaaaa cgagcgcaag cttccatata ccgaaaaaat tttttccatt
     5341 caaaagtttc accttcaatc aacagattat taaaaataac atctcctcta cttttagcaa
     5401 tagtaaaata gacggtatat tttttcgata ctgctttaaa agcacattgc ttttttgcag
     5461 aacgccaaaa agcgtctcta ctttcttgat gactttgaaa aaatggaagg ggttgttttg
     5521 cttgctggat aggcgaatac agcgacattt tctgtgaaaa gtaaactttt tttatctcca
     5581 tcgcgctctc ctttttcttc aagtatacca gtacgtcata aaaaggtcat gagtctcgta
     5641 ttcgactttg tagattccat aagaaaacaa ttcataaaac ctcctttaaa taaaaaaata
     5701 caaaaatcac aaaaattaat tgaaaatgag agtgttttca atgaaaacgt gattttgtaa
     5761 tggaatttta aaataaaaga aacaagcttt tttaatgcaa atgttgtaaa ataaaagaga
     5821 ttaagaggaa ataagaggag gaacatgatg actgagtcag tttatgtaca tattgatacc
     5881 acaagtaatg ctgttttaac aaaagggctg actgctactg actttgcaaa cagtatcgtg
     5941 cattttcctc aaaacttgct cttgcttgat ccatctgctt ctgctggaga atatgaatcc
     6001 catacagggc tgaaagtaat ccgtggtaca gaaaatattc agcgtttctt ttcctcttct
     6061 cgtaacaggc gtggattcga tgaattgaag tggatcgact ttactgattt aacgatgctg
     6121 aaagaattga cacctttaga aatttcagaa ttattatatt ttggtcatat gaaaacacat
     6181 cttcattcgc cgttttttta taaactacag aataattttg tatacttcga tcttaatgat
     6241 cagctgaatc ggatttatta tcgctattta gatgaatttt ataggatttt tgctagtaaa
     6301 ttaagttcga tcttgtcaga taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca
     6361 cctgttgaaa aaatcagtgt ggaattattg aaagaaatgc gtaatttgat gcaggaaggg
     6421 atcatttttt cttttgatca agttggtttg atcgatggta aatatcgtat cccaatctat
     6481 gtgattgaag attatatttg gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc
     6541 gctactttaa tctactctgt caccgatcga cactggcaat tggtagaaga tgccgatctt
     6601 tcttatctgc cattgaagta gatctatttc ttatcaaaaa ataaaaagga aacaagaaac
     6661 taggacatga ttctgtctca agtttcttgc ttctttttta tttacagctc ctgtcgttta
     6721 tctggcacaa aaatcgaagg agtcgtttgt tggatcccta ataaaacgat gatcgttaca
     6781 atagctgtac tcaacccgct ggcaccattc attacgaaag aaaagagcca aggattcatc
     6841 ccccatagtg catacgctcc ccagaaaacc actcctgcaa taaaatgcca aaaatatctg
     6901 gaaagtgcgc caatcaatgc accaaacacg atgctttttc taatttgttt cgtattatcg
     6961 tctacatagc ctttttggac atttgaagcc atgagccctg ctgcgcctgc aaaagtatag
     7021 gcaatcgggt actcaatcaa cacttgaacg acacttaaat aataaacttg tcccaacgga
     7081 aaatgtagga tgccccaaat gaaacctgcc agcatccctg cctttgtccc tcttcttaac
     7141 gcaaaaagag tcaatggaat ctgccctaat gaaatagaaa aactgctgcc gatctggatc
     7201 ggaatcaacg acaacaccat cgctaacgct gcaacaatcg ttccttctac ccaaactcta
     7261 ttttttccca taaaaaaact tcctcctttg aataaaaaag tcacaaagga ggaagcagct
     7321 ttcgaaaata gtcaaacagc tgtttattca caattcctac gctcgcataa ccgatacagg
     7381 tacgaagggt ttagaaatag attcaatctc agcccaattg gactcccctt tgtgagttta
     7441 tttattattt attttttgtt tcaaacaatg gactaactgg tttgttttca tggatacgtt
     7501 tgattgcatc tcccattagt ccgccaacac ttacttcatc gattttgtcg attttacgat
     7561 cttctggaag atagatcgag tctgtaacaa ctagacgctc gatagctgaa tcttctattc
     7621 tttgtaaagc agggccggat aatactggat gtgtacatga agcatacaca ctagttgcac
     7681 cagcttcttt caaagcattg gcagccaatg tgatcgtacc tgctgtatcg atcatatcat
     7741 cgatcagtac acaagttttg cctttgactt gtccaatgat attcattact tctgcaacat
     7801 ttgcttttgg acggcgtttg tctatgatcg caatcggaga tttcaaaaat tccgctaatt
     7861 tacgtgcccg tgtgacacca ccatggtctg gtgaaacaac gacaacatca tcgcctttga
     7921 ttcctttttc taagaaataa ttggcaataa gtggcgcacc catcaaatga tcgactggaa
     7981 tatcaaagaa accttggatt tgaactgcat gaaggtccaa agtcagcatt cttgttgcac
     8041 cagctttttg aatcatgttt gctactaatt tcgctgtgat tggttcccgt gcacgtgctt
     8101 tacggtcttg acgcgcatag ccataataag gcatgactac gttgattgtt tttgcacttg
     8161 ctcgtttcag tgcgtcaatc atgatcagca actccatcaa gttatcattg actgggctgc
     8221 tcgttgattg aataacatag acatgcgatc cacgaatact ttcttcaatg tttacttgaa
     8281 tttctccatc actaaattga gtaacagatg attttcctaa ctcgacgccg actgctgctg
     8341 caattttctc tgctaatggg cgattggaat ttaacgcaaa aatttttaat cttgggtcaa
     8401 agtaatgttt tgacatgggg acctccactt tctttttcac aactctagaa ttctacctaa
     8461 taaatagtag tcatattttt taaataaatc aagtgatttt gcaaaagtct tagtttagat
     8521 aagggagttt ttttgcatat ccttctttat tgacttgtct tgctctcgca attgccagcg
     8581 catattctgg aatattgtct gtaattgttg acccggcagc aatcgaactg tttttagcta
     8641 cctcgacagg tccgatgata ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg
     8701 tatgatgttt gttctttcca tcatagttga cgaagacaac accacatccg acattgattt
     8761 cttctcccag cgtagcatca ccaacataag tcaagtgacc gactttagtt cctttgccga
     8821 tttgagcatt tttcacttcc acaaagtttc cgatatggac gttttcgcca atttcagctt
     8881 ttggtcgtaa atgtgcataa ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa
     8941 tgactgagtt ttcaatgacg acgtgatcct caatcctact gtctacgatt tttgaatgtg
     9001 acccgatgac acaatcagaa ccaatgactg tatttccttg gatttgtaca cctgcttcga
     9061 tcaatgtgtc tggaccgatt tctacgcctg catcgatgta tgtcgttgct gaatcgacaa
     9121 agcttacgcc attgaccata tgcatctggt taattcgctt tctcatgatc tcatttgctt
     9181 tagctaacgc gatccgatca ttgactccca ttgattcttc aaaatcatcc gtttgataag
     9241 cagcaactgt atggccttct tcttttaaga tttcgatgat atctgttaga tagtattcac
     9301 cttgagcatt gtttgttcca actttgctta acgcatcgaa caaagcttcg ttgtcaaaac
     9361 agtaagttcc tgtattgatt tcttgtacaa gagcttcttc tggtgttgca tctttttgtt
     9421 ccacgatttt ttctacgatc ccaatatggt cacggatgat tcgtccatat cctgttgggt
     9481 tttctgcttg tgccgtcaag atcgttgcac ttgcattttt tccttggtga tattcaaaaa
     9541 gattgttcag cgtttcagta gtcaataatg gtgtatcgcc actgataact aacgttgttc
     9601 ctttttttcc ttccaagaaa gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt
     9661 ctgcttgtaa cgcatattta ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt
     9721 gtccaacgat cgtaatcact tcatctggtt tcgtttcact gacccgatta atgatatgtt
     9781 ctaccatagg tttaccagaa acgggatgta aaactttata taattttgac ttcatgcggg
     9841 tacctttccc tgccgccaaa ataattgcat aacgtgcgtc caactgcgtc actcctaaat
     9901 atagtttgct gatttcagtt tatcccagta tacccatttt ttcaacaaaa caagtgattt
     9961 tcttttatcc cagcgttttc tggataaaaa aatagataga ccataacggg cctatctatt
    10021 tggtaaccgg tgctctcctg ccaactcctc ggtcacaagt cacactactc ataaaacatg
    10081 aaaagctgtt ttttgaaatc gtttcttgtg acttagagtt aaacggtagg cttacaacct
    10141 cttcattaaa cgtttttcaa tcagctgacc ttcggtatta gctcaataaa cggcaaaata
    10201 tgaggagcaa tttttccgtt tattgagcta atactcagga caggacgctg atttcacaac
    10261 ctctagataa acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa
    10321 tgtggaaagc cattttcaca aaatttttct tactactcga aactaagcag ctttttcaca
    10381 acctcttatt ctgaaaaata atttcctggc acaaccgtaa tatttttcgt ttgagtatct
    10441 acatctttga cataaagcag tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt
    10501 gattcggcaa atacagtgat gccaacaagt tctgattcga attcttcaat caagctcttc
    10561 attccgttga ctgttccgcc gcctttcatg aaatcatcca ctacaaggac acgtgaacca
    10621 cgtttcaaac ttctcttaga cagttccatc ttttcaattc tttcagaaga tccagacaca
    10681 tagttgacac ttactgttga tccttctgtt atttttgagt cccggcgtac aataacgaat
    10741 ggcgcattca gataatagga cacagcttgg gcaataggta cgccttttgt ggctacagtc
    10801 attacagcat cgattttttc accaaggtat ttagaagcaa tgattcttcc aacttgacgc
    10861 agcagttcag gttctcctaa caaatcagag agataaacat atcctcctgg aagcagacgg
    10921 tcttgttctg atagacgttc tgctagagat tcaatatatt ttttcgcctc ttcatatgaa
    10981 atttcaggga tgaacaatac accacctgca gcacccggga ttgtttccaa ggttccatac
    11041 cctcgttctt taaatgtttt cttgatgatt cccaaatctt cactgataga agatttagca
    11101 gattcataac gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg attcaataaa
    11161 tactgtgtca tgtcaactag acgttcactg cgacgtactt tcacctattc caactccttt
    11221 taagaaattt tatcgtttta cattatataa gtttttcttc aaaaattcga gcattttatg
    11281 aaaaaataag aaaatagttc ggtatttatt aaaataaaaa gaagcctata aaaaattttt
    11341 cgtttttata aggcttcttt tctttataat ttagttattt tttcttctga aggcaaccaa
    11401 tattcgtttg atgatattta caatcaggaa caaagcaata aagactaatg tgattgttgc
    11461 tcctggaggt gtatctagat aaaacgagct agttagtcct gacagcatcc ctagaaatcc
    11521 tacacaaaca ctgatagcaa tcacgacatt gaagcttttg cctaatcgca taccgattgc
    11581 tgcgggaaga accatgattg cggatatcaa aagtgcaccg gcaatcggga tcatcacagc
    11641 aatagccaca ccagtaatca cattgaacat catcgacatc aaatgaacgg gtagcccatc
    11701 cacgtgggcg gtatcttcat caaaagtcag aacatacatc ggccgtttga ataaaaagaa
    11761 caatataccg atggcaacga ataagattcc taaaaagatg acttgctccc atgtaatggt
    11821 cacgatcgaa ccaaacagat aggattggat acttgttgct gaattccctc cgctcaaatt
    11881 catcaatact aacgctagcg ccaaaccacc agccattaaa atggcaatgg agatttctga
    11941 ataggttcgg tagatcgttc gcagatactc caatatcaca gctgctaata caacgactag
    12001 catggtcgta agtgtaggat taaggttcaa aaagaatcct aacgctaccc ctgctaaaga
    12061 gacatgagaa agcgtatcag ccatcagcga ttgtcttctg atcactaaaa acacacctag
    12121 catcggggcg attccggcaa tgaagatagc ggcaagaaac gctcttctca tgaattcata
    12181 tgaaagcaac gccattgaga atcctccttt ctcaccagtc ggatctgtct gtccgcatac
    12241 tgtttgatat cttcatgatc atgggtgatc attagaactg atttcccatg gacatgcgca
    12301 ctatgtcgga gaagacgata aaattcattt ctggattctt catccatacc tgttgttggt
    12361 tcatcaagaa caaataagtc agggtcagta gcaaaaatac gcgctaaact gattcgctgc
    12421 ttttgtccgc cagacaattc acctattcgc cgatttcgca tatcccacat ccctacagct
    12481 tctaaagcac gctgtacatg ttgatgatct ttttcagaga gacgcttgaa ccagcggcct
    12541 cttggatatc ttcctgaacg gactaattcg atcaccgtac taggaaaacc tgcattgaaa
    12601 gatgcaactt gttgaggaat ataaccgata cttaattttt ttccttcttg atttttctca
    12661 gcaatcttta tcattccttt tgaaggtttc agtaatccta acgatgcttt caccaaggtt
    12721 gacttggctg ctccattttc tccagttaaa atgacaaatt cacccgcatc tacatgataa
    12781 gagacatctt ctagtacagg ttcatcatca tagtaaaagg tcaaatcaga tatttcgata
    12841 taacgcacct tacaacctcc attctaaaac ttaattgata ctttctttta aagctttcag
    12901 atttttttcc atgacagaaa tataattttc gcctgctttg atttgttcat tagtcaagct
    12961 ttctaaagga ttcaacactt ctagcttcac tcctgtttca ttcgacaatg tctcagctac
    13021 cttcgaagag gcattttctt caaaataaat cactttgatg ttgtttttct tcacaaattc
    13081 ttttagctca gctaagcgac ttggtgtggg ttcctgatct ggtgaaacgc ccgaaatcgc
    13141 tacttgtttt aagtcgtatt gattcgctaa ataagcaaaa gcagcatgct gagtgacaaa
    13201 tgtgcggttt gttgcatctt ttagctcatc ggagtatttt tgatccagtg cttcaagctt
    13261 tttgatataa gcatcggcat tcttcgtaaa agtttccttt ttatctggat atttttcgcc
    13321 taattcttca gcaatcgttc tgacttcttt tatagctaat accggatcga gccaaacatg
    13381 tggatcgtat tcatgtgaat gaccatgccc ttcttctgat tcttcatgtt cgtctgcttc
    13441 ttgcgattct aaacggtcga ttcctttagc tgcttcgatc atcaaggtct gtttgctatc
    13501 cactgaatcc tttaacgaat ccacaaaaaa ttccatgtct gaactattat acacgaaaac
    13561 atctgcatcg gaaatttctg ctctttcttt tgcacttggt tcgaaatcat gcggctccgt
    13621 tccagcaggg atcaaaagtt tcacgtttcc ttcatcccct acgatctctt ttgtaaaatc
    13681 atacatcgga taaaaagtcg taacgatcgt caaatcctct tttttgtctg cttccttgtt
    13741 cgtatttcca catgctgcaa acagtaaagc acctatcatt accgtagctg tcaaaagata
    13801 ttttttcatt tatatgctcc tttcaatgaa caaatcggaa ttttacgatt tattaacgtg
    13861 aactaatgta tcaaaataaa aaaacacagt caatagaaat ccatcaattt ctgatttaag
    13921 catgtatatt cttgattaaa tcgtaaaatg gatgagttgc tgctcatatc tagccatttc
    13981 actacaataa aaaactgaag aataccattt taggattcct cagtttttca tcacctattt
    14041 tacttcttat attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta
    14101 acccattata tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc
    14161 cgcttccact catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat
    14221 ggatgactgg atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca
    14281 tctgctgata atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga
    14341 tggcttgcgt atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga
    14401 ccaacacaac ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac
    14461 cgacaaatgc cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga
    14521 tttctgccag ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag
    14581 cagctgcaca gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa
    14641 caaagatctt gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat
    14701 aaacattgtt ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt
    14761 cttctgttat ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt
    14821 ccaactcatg atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg
    14881 ctggcgcttt ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta
    14941 ttctatcata gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat
    15001 cgtttcaaca gctttcacag aattttctct tcataatcca aacaagttct agtgattctt
    15061 ataattctgt gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca
    15121 gactgagtag ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac
    15181 gtccttccca atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga
    15241 aaaaattacc ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg
    15301 tttctccaac attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct
    15361 cgtatacaat aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta
    15421 agacccgcaa caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc
    15481 caacgacttt ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat
    15541 caaatcgtcc cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt
    15601 catttacagc ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt
    15661 ctttcatcaa acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta
    15721 gatcaacaat ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca
    15781 ctacgtagct ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt
    15841 tcataaactg attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa
    15901 caaagcattt gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt
    15961 ggtaataaaa ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt
    16021 atgtttccaa taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat
    16081 cttcagctaa atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa
    16141 gccctgagaa ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac
    16201 cgactaaaaa gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga
    16261 acaagctagc atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt
    16321 cttttctgga taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag
    16381 ataacgtacg tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa
    16441 atgccttttt gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca
    16501 atagattcac tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc
    16561 gactgattgc agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat
    16621 agacagcact tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat
    16681 ctaaaaatcg attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa
    16741 aaggtagaac ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc
    16801 gaacaggcaa ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg
    16861 ggaccatcgt cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca
    16921 tcgaaatatc tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc
    16981 attgtccaaa ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca
    17041 atgtctttct ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga
    17101 aaagccagat ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa
    17161 atgtaagaaa cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag
    17221 tggctaaaac catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc
    17281 ctaccacacc accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa
    17341 gaaactcttt tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc
    17401 aatcatacaa caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg
    17461 tcatgcggct ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa
    17521 tatttgcgac ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa
    17581 tcagactatg ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc
    17641 attcctaact tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt
    17701 ccactttaga cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa
    17761 ggagaggata acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt
    17821 gatggtttat taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat
    17881 tttgaaaatg cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag
    17941 gaagttgcat cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa
    18001 gtattggaag aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga
    18061 aaatcattaa cagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg
    18121 atgccgaatg tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct
    18181 gccacggagg aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat
    18241 cctttagcag aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc
    18301 gtttacatgc tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag
    18361 acagccactg agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt
    18421 gataaaaatc catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca
    18481 ggtgtagttg ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg
    18541 acgatcgaga aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg
    18601 ccttacaaaa aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta
    18661 tttagcataa tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata
    18721 atccagttct tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc
    18781 cgagatttct tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact
    18841 tgattctaca ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta
    18901 gttttctaat gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc
    18961 tacaaggaca gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt
    19021 gattactact gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga
    19081 tccttcaatc tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc
    19141 taattggata tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac
    19201 cgaatgcttc aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt
    19261 gattagatct ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc
    19321 gcgaatacgt tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg
    19381 atggattcgt ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat
    19441 tggatcgaat ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc
    19501 agtcagcgtc tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat
    19561 ttcatcattc ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca
    19621 gcgttgaatc gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg
    19681 ctgttcagat tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt
    19741 catgatgaca tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg
    19801 ttgatcttca atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga
    19861 actaagtgct tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt
    19921 acgattcagc gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt
    19981 actttctctc agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc
    20041 taacaatgct tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc
    20101 agctgatgat tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc
    20161 taaaccaaga ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc
    20221 cacctccata ctatcttttt catttttatc atctatacag cttctatgat aaattattct
    20281 aaactcgtca atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct
    20341 aatgtttgta ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc
    20401 attaaacgat ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg
    20461 agaaactttt ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc
    20521 actcgtataa ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga
    20581 gagtacctac tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa
    20641 ggcaattctt cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa
    20701 gctgctctaa ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt
    20761 ttagcgtttt cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt
    20821 ttatcaacaa tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct
    20881 aaaccataca tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg
    20941 actttgatct ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga
    21001 cgtacttcca aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct
    21061 ggattcccaa acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt
    21121 tttgttttgt tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt
    21181 aacccgacat gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca
    21241 actgaatcga ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct
    21301 agaccttgtt ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat
    21361 ttttgtgcat attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca
    21421 tttttttgta cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt
    21481 ccatatactt cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac
    21541 gcaagagagc cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata
    21601 atagagcctt taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta
    21661 cgatcatctg ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta
    21721 tccttatcat atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt
    21781 tatcattttc ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat
    21841 ctgatagcag cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat
    21901 gaaatagcga tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact
    21961 ccggtgaaag ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc
    22021 tccacacatt ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga
    22081 aaaccagctt ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca
    22141 ccagcaatgt cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt
    22201 acagtctgct tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg
    22261 ccgtatccat aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga
    22321 gcttgattcc ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt
    22381 ggtttggcat atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca
    22441 gtgaccagtc gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct
    22501 tcagaaggaa aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac
    22561 tcattaggcg gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct
    22621 aaagcaagcc ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc
    22681 agattgtttt tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat
    22741 ccttccgtat tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg
    22801 tcatcttctg tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct
    22861 agagcaatca actcttctaa acgctgctga ttatctccta caactgtttg ataataaact
    22921 tcgataccta gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt
    22981 cctagtaaaa gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt
    23041 atatagtata cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac
    23101 aaaaatcttg tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt
    23161 cataagtcac aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact
    23221 cagagttaga cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga
    23281 acacaccgct gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag
    23341 cataaagcat gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa
    23401 acagcaagat aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag
    23461 ccatgacttc tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac
    23521 agacaatgat cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag
    23581 cagtcataac tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa
    23641 ggcctcgcgc acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa
    23701 taactgcacc tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc
    23761 aatctaaagg taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag
    23821 ttaatttatt tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc
    23881 agcgaagttt ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt
    23941 tattgaaatt gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc
    24001 cttctttttt atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg
    24061 tattgaagag taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt
    24121 acggattctc cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca
    24181 tttgttgctg tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga
    24241 taaacatagg cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca
    24301 agggttacag gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct
    24361 cctgtagcta ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa
    24421 gactctttgg tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact
    24481 ttaagcgaag aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata
    24541 tagcccacga caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt
    24601 gtccaaaagc gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact
    24661 gtttcaggct gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca
    24721 tcgactaaat ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc
    24781 acgtaaaatt taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt
    24841 ttttgaattt ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct
    24901 tgccgtaatt tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg
    24961 attctccgaa gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag
    25021 tcatgtcaca gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca
    25081 agtcacaata tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga
    25141 gttaaacggc aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta
    25201 ttagctcaaa aaccggcaaa atataaggag atatttttcc gt
//
LOCUS       DACJGB010000041        24853 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.041, whole genome shotgun sequence.
ACCESSION   DACJGB010000041 DACJGB010000000
VERSION     DACJGB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.041"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(324..1106)
                     /locus_tag="IYI34_001710"
     CDS             complement(324..1106)
                     /locus_tag="IYI34_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559580.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1171..2007)
                     /locus_tag="IYI34_001711"
     CDS             complement(1171..2007)
                     /locus_tag="IYI34_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0559581.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2025..3434)
                     /locus_tag="IYI34_001712"
     CDS             complement(2025..3434)
                     /locus_tag="IYI34_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR0559582.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3448..5271)
                     /locus_tag="IYI34_001713"
     CDS             complement(3448..5271)
                     /locus_tag="IYI34_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0559583.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6114..6440)
                     /locus_tag="IYI34_001714"
     CDS             complement(6114..6440)
                     /locus_tag="IYI34_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559584.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6486..6848)
                     /locus_tag="IYI34_001715"
     CDS             complement(6486..6848)
                     /locus_tag="IYI34_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HAR0559585.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(6885..7099)
                     /locus_tag="IYI34_001716"
                     /pseudo
     CDS             complement(6885..7099)
                     /locus_tag="IYI34_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7227..7562
                     /locus_tag="IYI34_001717"
     CDS             7227..7562
                     /locus_tag="IYI34_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0559586.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7638..9635)
                     /locus_tag="IYI34_001718"
     CDS             complement(7638..9635)
                     /locus_tag="IYI34_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559587.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9733..10008)
                     /locus_tag="IYI34_001719"
     CDS             complement(9733..10008)
                     /locus_tag="IYI34_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559588.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10240..10974
                     /locus_tag="IYI34_001720"
     CDS             10240..10974
                     /locus_tag="IYI34_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0559589.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11013..12155
                     /locus_tag="IYI34_001721"
     CDS             11013..12155
                     /locus_tag="IYI34_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0559590.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="IYI34_001722"
     CDS             complement(12250..13641)
                     /gene="sufB"
                     /locus_tag="IYI34_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAR0559591.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13646..14116)
                     /locus_tag="IYI34_001723"
     CDS             complement(13646..14116)
                     /locus_tag="IYI34_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAR0559592.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14103..15338)
                     /locus_tag="IYI34_001724"
     CDS             complement(14103..15338)
                     /locus_tag="IYI34_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAR0559593.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="IYI34_001725"
     CDS             complement(15335..16621)
                     /gene="sufD"
                     /locus_tag="IYI34_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAR0559594.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="IYI34_001726"
     CDS             complement(16634..17407)
                     /gene="sufC"
                     /locus_tag="IYI34_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAR0559595.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17604..18452)
                     /locus_tag="IYI34_001727"
     CDS             complement(17604..18452)
                     /locus_tag="IYI34_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0559596.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18476..19162)
                     /locus_tag="IYI34_001728"
     CDS             complement(18476..19162)
                     /locus_tag="IYI34_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0559597.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19155..20189)
                     /locus_tag="IYI34_001729"
     CDS             complement(19155..20189)
                     /locus_tag="IYI34_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0559598.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(20584..20922)
                     /locus_tag="IYI34_001730"
     CDS             complement(20584..20922)
                     /locus_tag="IYI34_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAR0559599.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(20924..21280)
                     /locus_tag="IYI34_001731"
     CDS             complement(20924..21280)
                     /locus_tag="IYI34_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAR0559600.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21286..22473)
                     /locus_tag="IYI34_001732"
     CDS             complement(21286..22473)
                     /locus_tag="IYI34_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAR0559601.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22664..23509)
                     /locus_tag="IYI34_001733"
     CDS             complement(22664..23509)
                     /locus_tag="IYI34_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR0559602.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23743..24708
                     /locus_tag="IYI34_001734"
     CDS             23743..24708
                     /locus_tag="IYI34_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAR0559603.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7264 a         5228 c         4223 g         8138 t
ORIGIN      
        1 gttatcttta gaaccatttc agatacatat gaaacaatta catatgattt tttcataaaa
       61 aaacagtact gtctagtagt ataaaatata caaatttctt aaaaattttt tcactaaaat
      121 cactacctga aacagtctaa aacacttata tatcaacgtt taatgaaact atctccgttt
      181 catttcagat acaaataaat atagtcgggc tccaaatccg aaaaaaaata cccccagtag
      241 cttatcctaa gctactggag atgtataagt acggtcatat aacaacgggg tagggtaaat
      301 aaatgaaaaa gatggaagaa tcattattca ataacttttt taaatacttc gttgtaatca
      361 gttacctgtc cgttcttaaa taccacagaa taatcttttt tcccattttc aagtaaccga
      421 agtggaatgt ctttaccaac ttgctctgtc actgcattac tgatacctgt tagattagct
      481 aataaatctg ataaagattg agcatctgta gaaatctctg tatcagtatt tatcgtgatt
      541 cctttatctt catttacaag cttccaagta ggtggtatag ctgtgtatgt ttcgatgaat
      601 gaatcaattc tttcatctat cgtcttccca gtatctactt ctgtctcatt actcttagat
      661 gaatcgattc tttcatccat cgtctgctcc gtaactgctt ctgtctcatt attctcaccg
      721 gaaactctct tcacacccaa acttatatat tgtccatctt tagtgtgcat agttaaaatt
      781 cccacgcttt tactaataaa ttcactataa ccagttcttt caatagagac ttcaatgtat
      841 ttcacactat catactcaaa agaaaaatag acatgtaaaa gcattttgtt gtttacatct
      901 acataataat cctttgactc tacttgagac cttcctatac ggaatacagc tttactgtct
      961 gatatctcta taccagcatc cttattatta atctgccaat ctccaagtag ctcttctggc
     1021 aagctagtag tttcttgtga acactttacc acttcactgg tagaggtcga aacattcacc
     1081 tccgcactgt tagaagaatt ttccaaggaa tcaggagcct tagcacaacc aacaagtctt
     1141 tatatattgt gcagccgata ttttcttcat tcagtacacc cttgaaatat gcagtgttag
     1201 ataaagcagt tcgtcctgat ttgctgaggc acccatctct tcttgaacgt actccttgat
     1261 tttcaaggcc gcccgatact ctttcggaaa acgctgcttc actgaagcca gcaaaaaatc
     1321 atccttctcc ggcaactctt ccttcttaat cattcgggca ataaaaaact gtaaatgcgt
     1381 cacaaatctt aagtaaagca tcgaatcttt atcaaccttt ttttcgggaa acagatatcc
     1441 tagcatagcc aagatatcct ttagtgctct taccgagagc atggtttctt ccatatttgc
     1501 ttgttcactt tgggcattta caatgtgaaa cgcaatgttt ccagcctcct cttctggcaa
     1561 ctgaacattc atttttttgt tgatcaattg gatagcatag cagccgatct caaattcttt
     1621 tgggtaatac ttttgaattt caatcagcag gcgattttga aaaacaatgc ctttttgact
     1681 gcgttcaatg gcatgcttca agtgatcaac caaggtaaaa aatagacttt tattcagctt
     1741 aaagtcaaac ttggcgttgg cataatcgat aattcgcttg acgcaaataa ttagatcatc
     1801 cggagcagcc tcaaacagtt taatgtaatc cttttttaga gcatcagaaa gtgaatctaa
     1861 tttaaatact ttttggattt tgtcagccgg aaccaaatcg cctttcttta gttgaaatcc
     1921 aatccctttc cccatcaaaa ttacttcttt gccttgctta tctaagcaaa gaattaaact
     1981 gttgtttaac gttttaatta ctctcattag attcaccgtt ctctctaagc taagccattt
     2041 cttgcgatta ctttttgata ccaaaagaag ctttcttttt ttattcgttt taattcttta
     2101 agattctcat cggttcggtc aacatatata aaaccataac gcttattgaa gccttgatgg
     2161 gtactaacaa catcaatcgc tgaccaaggt aaataaccaa aaacatctac accatctgtt
     2221 aaagctgctt gaatagccag aatatgtttg gctagataat caattcgatc ctggtcatta
     2281 attcgtctat cttcctcaac tatgtcgggt gccccatagc cattttcagt aattaaaata
     2341 ggtaaatcat agcgatcgta caatttcctc aatgttaatc gtagtccagt agggtcaact
     2401 acccagccat atggcgtttt atctacataa ggattttcag ctgcccggta gacaccttct
     2461 tctcccaaca tgatctgttg atcccccgct ctagcggaaa catctgatcc gtcatttttg
     2521 ctggcagcga tggtggcagt tgaataataa ttaatcgcaa taaaatcggg tttaccggag
     2581 gataaaattg cctgatcctc tgcctcaatt ttaggatata atccacgatc attcaaatag
     2641 ttttcaaaca aataattgta ttttccccga actgctacat ctaagaaaga ccagcctctc
     2701 aatgtttccc aattatgggc tgcaattgca tcttctggac tggaggtagc ttgatacata
     2761 gccgttaaat tgattgctgg accgattttt cctttaagat tcaactgatg atacaagtga
     2821 attactctag cttgagctaa taaaacatgg tgattaattt gaaagagttc tttttttgaa
     2881 ggcagctctc cacttggtgg caaaccaatc gctccaggat gaaggatcat cgtgttttgc
     2941 tcattaattg tcagccaata gttcaccttg tcaccatact cagtaagcaa tacctgtaca
     3001 tagtccacaa aataatcgat agttgctcga tttaaccaac cgccctgctc ctctagaaaa
     3061 taaggtaaat cgaaatggta catggtgaca atcggttcga ttccctgatt caggcattca
     3121 tcaattactt gatgataaaa agaaattccc ttttgattgg ttttattatt ttcaatatcc
     3181 ataattcgcg tccaagcgat ggaaaagcga aaagctttta agcctaattc tttaaacagc
     3241 tgtatatctt ctttatagtg atggtaaaaa tccgaggcaa cttcaaaatt agctacccct
     3301 tctgtatgct gatacttgtc aataatagac ggttgtttcc catcctcctg ataggcaccc
     3361 tctgcctggt aggcactaat ggaggcaccc cataaaaact tctctggaaa tgctgaaagc
     3421 tgtttgtatt gcacaaaaat cctctcctta actaatttgc atcaatggtt cgtgttcctt
     3481 taagcttccg cttgcttcac taattcctgc gtaattttca ctgttggtca ctaccacaat
     3541 cactgtatca tcgtagccag cgtttctaat ggcagcgaaa tcggcgttga ttaataattg
     3601 tcctgctgac acctgatctc ctgcttttac aaatacttca aagggttccc cttttaaatt
     3661 cacggtgtcg atccccacat ggatcaataa ttcaatacct tctttagaac gtagaccaat
     3721 cgcatgttta gattctaaaa tagtaatgac ttctccctca acaggtgaat aaatgctgtt
     3781 ttcttctgga tgaatggcta cgccctttcc caatagtccc ttagaaaaga cagcatcgtt
     3841 cacttcagaa ataggacaca cggttccttt taccggattt tcaacaatga ctgcttcggc
     3901 tattgccgtt tgctctgatt catcagaagt atcgtcaaat ccaattagat aagcaatgat
     3961 aaaggttgca acaaaggcga ttacacacgt aataattgcg tgaacaatat tcattggatt
     4021 ttctccaata aaggctggta aggctgctaa gccaggggaa acaaaggcat atctgactaa
     4081 accgctcaag ccagcataca aaccagcaat tgcaccacca atcatggaag caactaatgg
     4141 ttttttcagc ttcaaggtta ctccatagag tgaaggctca gtaattccca ttaaagcagt
     4201 gatcccagtg gacacagcta gctgcttcaa ctctttgttt ttagttttcg cggctacagc
     4261 aaaggtagca cctgattgag caatattaga agccagcatt cctggcccat tgatcatttc
     4321 agaacccgta ctgctcaatt gcatagtagc gattggcgtc attgcccaag ctgtacccgt
     4381 aaccaccaaa aatggctgca aaccacccat caaaaatgga attaaccaag ctgctttgcc
     4441 gttaatccat tcagccccca ttgctataag atcgttcagc aacgttccaa acggtccaat
     4501 gacaaccaac gctaaaggcc ccataattaa aaataccagc atcggcttag taaagaattt
     4561 aataaacgaa ggcgaaattc gatccgcaaa gggctctaca taggacatga tccaaacagt
     4621 aaagataatc ggtaaaactg atcccgcata atctgcagct aataccggaa ttcctagaaa
     4681 agaaacggca tcgccagtag aaaaaagctg tgaaatattt ggatgaagca aaatgccagc
     4741 aacagtaatc gccaaaattg ggttgacatc aaactttatg gctgccccat aggctaatag
     4801 tactggcata aaataaaagg ctgcatcagc aatagtattc aaaatgatgt aagtttgact
     4861 actgtctgat agtacaccag tcaaagtcaa gacggccaaa atggctttta ccatcccggc
     4921 acctgttata gctggaatca tcggtgtaaa ggtggtagaa atgacactaa tcaatcttga
     4981 aataatactt tcttgattgt ctgaagaggg gcccttttta attgaagttc ccacttcctt
     5041 ttgaatttcc ttgaaggctg tttggacttc attgccaatc accacttggt attgtccacc
     5101 gctatccacc acactaatta caccaagtgt tttttctaag gctttcgtat cggctttcct
     5161 tttatctttg agttcaaaac gcagtctggt ggcacaatgg gttaagctga ccacattttc
     5221 ttttccccca acctgttgca atacatttct tcctacttct ctgtaatcca ttattctctc
     5281 tccttaaagc agtaaaaaag accaagtcaa accacgcccc aatcaacatt gaagtgttag
     5341 tttgtcttgg tcttgcctgc ataatcagta acatgccaat aacgttattt tgctgtaatc
     5401 caataatagc acattatgat agcgcaatca acagggaatt taatttttta tcttttcgtt
     5461 tgaccgtata gggtaatata acagttagat cacatcaact agggtatatc tgcgtatact
     5521 tactgctaaa ataagtatat ctaatattaa ctataccagt aaaaccattc ctacataacc
     5581 agcgggaaat tcccaaaatc gtgaaagcat caaaaatgca tataacggat cacacaactt
     5641 caacaattct gctcttggga aaaattgatg aatttggtga gtaaaggatg gaataaacag
     5701 cattccaaca atataggcaa caatagtgat acttgttttt ttggtaaaaa tcagtaagca
     5761 atagccaatt tcaatcattc cgagatataa aggtatcgat agaatgattt gggctagtat
     5821 tcttaaaaat taactagcaa atgtttaaac tgatttattt ctaatctaca tgtagtaagt
     5881 atttcaattc caccaattat caataaaaat accaaacata tccataataa ttttttcttt
     5941 ttcatttctt ttatattgtt ataacagtga ataacataaa aattcccgac aatcattgta
     6001 atattttttt gtcacctgta ttagtattta atttatagga aactataaaa actccctagt
     6061 gaatttacac ctttgggagt tcctgtttgg acggtatgca taatacattt aaatcaattt
     6121 aacattaaca cctcagcata tggtatctcc acatatatac ggtctttctt tggatagtac
     6181 tcaatattta tgttgatagc acttccattg tctaatatag ctttagcccg gtatgtgtta
     6241 tctccatttt catgagtaat cgtcacatca tcacatgatc gatttaagtt gtactgttct
     6301 tgtaaaatct gtgttaccaa tggtttcgat gacttctcta atccagatgt acctgtcatt
     6361 tttgaaaaaa tgatacctat aatagccaag acaacgataa ctataatgat aaacttcttg
     6421 ttcatattta ctttattcaa ctttttatct cctatctcca aaataatttg ctcttatccc
     6481 tcttatcagt acattgacat ccctaaaagt acaccaaaga ttgctccagc tacccctgct
     6541 atcatcaaaa tcgtacccat ttgttcatct ttatctcgat ataagtagcc cataataaca
     6601 gcaacagcac caaatacaac aggaataaat gctaacgcta ctaccataga aatccaacca
     6661 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     6721 attgtctgct cagtactttt ctttactgcg tctgctccag ttgtctcatc tgtttcttgt
     6781 agtgctccgc aaaactggca atacttactt tcatctgtta tttctttgcc acattttcta
     6841 caatacatgt cttctcctcc taaaaacatt caattgttta ttagttaact gtttacttta
     6901 ttaaatacat cagaaaacag ctcattcatt ggtacactta atatctcaga aagcatcttc
     6961 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     7021 acattcattt ttttgctaac tccgtttgag aaagtccttt ttcttttctg tatcggctga
     7081 gattacttac ttttttcatg attctcccct cctgttttat tatatacaac aatatgatgt
     7141 atttcaactc ataaaatata aaaaaacttc aaataccatt acaaatacaa caacttgttg
     7201 tactatactg agatgggggt gtattaatga aacagaaaca atatgcaaag gtcattagag
     7261 aaaagcgaaa agctctcggg tggacacaaa aagaattagc tgaaaaaatc ttctctacac
     7321 aacaggcagt agcaagatgg gaaaattctg taacagagcc taacctcgat agcttaaccg
     7381 ctctttctag agccctagga acccctgtaa gccattttct agacaatgtt gtcgtagatt
     7441 atgaggaaga gtttttggct ctctatcgtt ctcttagcac tgaagatgca gttcgaacaa
     7501 ttgactatat gaagctatta aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt
     7561 gaaaaataaa taacccaccg aactttaacg gtgggtgtat tgagatgaaa taagattaag
     7621 agctatggat aacctgtcta gtttaataca attgggtctt ttctcttcct tactctccgt
     7681 ccatcatctc ttgttacact tgtaaattga taaagaggaa ctaattcaac tcctgataat
     7741 atcaattctt ttattacttc atctatccct gctccttttg ctaggtagac taaaaaacta
     7801 ctacttgctt tcacattgta tagtctctta atatccttag aagctcgttc aagagtgaat
     7861 ccttgtttaa caaagtagcc tctgttttct tttaaagcat tttccacgct gttattccca
     7921 acattacgta gacgttcaca aacagtgtta gtaagttctt tctctgtcat tagatatgtt
     7981 ccttgccacg ataagccctg taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc
     8041 ttgctaagtt ctgatcggga attttgagga aatactcttt tagcctcttt ttcgttaaaa
     8101 catatcttaa cccattcata agaaaaatat tgatatgtaa aatgaaattt ttccagaagt
     8161 ggaaggatat cttcattcaa tttttgaatc acagaagttg cattaaaaac gtctaatcga
     8221 tagacattgc ttctcttatg attaaagttt cctaacgctt tctcttcctt taacctattt
     8281 aggtattcga gcatcttcaa aggaacatct tcatcatcta atttttttat caatcctata
     8341 aaagtcataa gtgatagtaa cttattacat ttatctatgt cataagtttt accatagtaa
     8401 aaactcattt ttttagctaa ttgttccccc gacaaaatac aagtcacact ttccttacta
     8461 tcatcaaaaa aattaacatc agaaagcaag acatcataga cacgtcgcat ttcctctaga
     8521 taattcctta ttgtcctgaa aagtttgtct ttcattgatt caagagtgtc aaaaagtact
     8581 tttgattcct ctttcaattt tcgaaaatgt gtaggataca agtctaggtt ctctaatagc
     8641 caattaaatg tttctacccg actgtctttc tttaaaatta gcatcacaac tccaataaaa
     8701 ttttcactga tatcacaatt actggaatga caataaaaaa tggaatgccc agttttactg
     8761 gtaaaaatac tagcagaggg attatgatcg ttgtgaaaca gacatcttaa gtttttactt
     8821 gacttaagta atttattcat atcaatggtt aacaatcttt cataaatctc attaaaagac
     8881 atgtcggagg aatcaaacag gcagtcttta aaccactgtt tcatttcttc tttgtcgtca
     8941 ttttttatca acttaacaaa atcggtttca ttgactagtt catactctct ccctgaatca
     9001 ctttgacaat ttttagaaca tgtcatggta aaatcaatcg gaagctcagt aatatctaac
     9061 atgttgtctt cattaattat tatcactgca tctgacttat atcctccaaa aaacattcta
     9121 ttcgaagcat ttacattgtc atcacacccg gggatgttct ttttcaagta ttcgtaaaca
     9181 gcaaccattt taccatagtc agtaattgct tcttttaata gaaagacaat cttgtatttt
     9241 tctagatttt cagagtaccg aatacttttt tgtatcgcaa acgcagaatt atgaactaaa
     9301 ttgatagata aaaccttttc tatgcttaag tatctactat catctttact caaatctact
     9361 accttcccct tctcatcttt aacagtgtta tcgacatcca gaataattac tctggtttcc
     9421 acaaaactct ccttgtcatt gcctttattc tttgtcttgc ttggaataaa tgattgtgat
     9481 gaatttagta tctctacaaa ctcatctctt gtaacttctc tatagttatc taaacaccta
     9541 tctcttacat cagttttttc tttttttgta actggcttct ctaataattt ttctttatct
     9601 acataaattg gaatattaat cattgatgtt tccatttgtg tttcctcctt ataaaattct
     9661 gttaaagact aaaacataca gataataaaa acaaactctt tattactata tgttttagtc
     9721 ttatttgttt actcaacctt tataccgttc catgaaacta tcaatatcag attttttaaa
     9781 tctaatgacc gtatcaattt ttataacagg taacccgtat tgagaaacat attttctaat
     9841 cgttcctact gatacaccta gatattcact tgcctgctga tagtccatat actctagagt
     9901 gactccagaa ataatgtctt ctagcatagg ctttagcact gtaattatct ctttcacttg
     9961 ttgttcaact tgtatagaaa caatgctatc aatgatttgt agagacatat ttccccctcc
    10021 tttcgtgatt gcttgaacaa attttctaag aattatcttc gtattttttg ctcaaaataa
    10081 aagtattttg ttattaaccg attaatcaaa caaaaaatga ttaccagttc atcgacatac
    10141 acataatacc tcgataaaaa agaggtgtca atatctttgt aaaatttatt tacaacaccc
    10201 ctttttcgag gtatactaat tttaggaggt gtggtcaata tgataaaacg tccaccaata
    10261 aattatttag aacgaaaaaa gatactcgga acaaaaataa aagctataag aaagagtaaa
    10321 aaattaacac aaccagcatt tggtcttatg attaacaacg gtcaattaat tgacaaaaaa
    10381 actatctacg aatgggagaa gggaacctat ttaccaatac cagagcgatt atcccgaatt
    10441 gctgatttgg gaaatatgag tatagaggag ttagtttgtg gcaatgtaga agaatatatt
    10501 ttgggaatta ttctttatag agatagcatt gtactagatg gtataacatt tccagataaa
    10561 aacctgttcc agcatcttcg tcaacagttc ccaccagtac actctaattt agatacatgg
    10621 ctggatagat attccaaatt agaaccagag atgcaagaat ttattgctaa caagacttgt
    10681 aataaagtta aaaatgaaaa aattagtctt ttcaatatct taaaaataga agaattattc
    10741 atcaatgcta ttgtagaaga atttgacaat aacattcttt tcctcactag cagtatagaa
    10801 gaactactag aaagaatggt agatgaatgg ctacctattc aattgaaaga catgtcttat
    10861 cccgaagaag ctgtgagaga aattactgat aatataaaca agctagaaca aacgatttca
    10921 tcaattggga aaaaatacac taagaaaaaa atgaagggag gtgataccat ctagtggtaa
    10981 gtactccttg taaactaaga ggagatgatt tcatggcaaa aatatcaaag tacaaaaaga
    11041 aaaacggtca agtggcgtgg atgtataaag gacacattgc caccgatcca cgaactggtg
    11101 aaaaaataaa tactacgaga agaggtttta gtagcaaaca agaagctcaa aaagattatg
    11161 acgagtacag acatcaaata ttatacggtg ttaaaaagaa agcacctgat atgacatttg
    11221 aagacctgta taatgaatgg ataactcatc agcgtaccag tgttaaagct tcaacaatcg
    11281 caatctcagt aagatacgca aataatcaaa tacttccagc atttggtaag cttaaactat
    11341 cgaatatctc tgtcccatat tgtcaaaaag tggtagatga atggcacagt aaatatgaaa
    11401 gttacgatta tatgagaaaa cagactgcac aaattttacg ctacggggtt gcaatgcaat
    11461 atattgacaa taatcctatg gaaaaaacac ttcttccacg aaaaaaagag tatgagaaaa
    11521 atcgtaaatt ttactcaaaa gaggagctta ataatttact ggatgctttt aaagattttg
    11581 gtaacatgaa acaatacgcc ttcttcagat tattggcata cacaggtatg agaaagagtg
    11641 aagtactcgc tttacagtgg aaagacatag acacgtttaa taaagaacta catgtcaata
    11701 aaactcttgc tgtagatgaa tttggtaaag ttataattca atctccgaaa acaagagctt
    11761 ctagacgagt tatctcttta gatactgaga ctctttctat cctcaacaac tggaaattac
    11821 agcaaaaaga agagtattta aaacttggat acaatactag ctctaaagag caacatgttt
    11881 ttacaacagt taaaaacact ctatacattc caaacactgt taatgattgg ttaagatata
    11941 ttttgaaaaa atataacttg cctcgtatta cacctcatgg gtttagacat acacatgcta
    12001 gtttattatt agaagcagga gaatctgtca aggtagttca acaacgatta gggcatgaaa
    12061 atagtaaagt gacattagac atctatgcac atattacaaa taatgcacct aagaaaacag
    12121 gtcaagactt tgctgatatg atggctcatc agtaaaagag ctggtcaaaa tctggtcatt
    12181 tctagtatac aggcaaaaca aaaaagctag aacccttata tatcaagggt tctagctcct
    12241 ataacaggtt tacccaacac tgccttccat ctcataactg atcaatcgat tgagttcgac
    12301 tgcatattcc attggaagtt ctttggtgaa tggttcaaca aagcccatga cgatcatttc
    12361 agttgcttca gattcggtca ggcctcggct catcagatag tagagttgtt cttctgagat
    12421 ttttgagact ttggcttcgt gttctaaaga gacttggctg ttgtggatct cattgaatgg
    12481 gatcgtatcg gatttcgacc attcgtccat gatgatcgtg tcacattcga tatgagagat
    12541 cgaacccgca ctgtttttgg caaaggttac ttgtcctcga tagtttactt ctccgccgtc
    12601 ttttgaaatt gatttagaga cgatagagct agatgtattt ggtgcattgt ggatcatttt
    12661 tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg atggagagca tggtgcctcg
    12721 tgcacctttt ccatctaagt aaacacttgg gtatttcatc gttgtttttg cacctaggtt
    12781 tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt gctcgtttgg ttactaagtt
    12841 gtacacattg tctgaccagt tttggatcgt tgtgtaacgg caatacgcat cttttcttgt
    12901 aaagatttct acgatggcgg catgcaggct gttacttgaa taagtaggtg ccgtacaacc
    12961 ttcgacatag tggatgcttg ctccttcgtc aacaatgatc agcgtacgtt cgaattgccc
    13021 cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga acatcgactt tgacgccttt
    13081 tggcacgtaa ataaaggtcc cacctgacca tacggctgaa ttcagcgctg ctaatttgtt
    13141 gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat aattcagggt attctttcaa
    13201 agcagagtca gtgtcagtaa agacgattcc taacttttcg aattcatctt tcatattatg
    13261 gtacacgact tctgattcat attgtgcgga agcaccggct agataagctc gttcagcttc
    13321 tggtatacca atcttttcaa atgtatcttt gattttttct ggtacgtctt cccagtcacg
    13381 agcgggtttg tcacttggtt tttggtaata tttgatggca tcgaaatcga tgtctgaaag
    13441 gtcggggccc catgtttgca ttggcatttt gttaaatgtt tctagagatt tcagacggaa
    13501 ttcaagcatc cattctggtt cgcctttgat tcttgacatc tcacggatca cttcttctgt
    13561 caagccttct cctgtactga aaaccggttc gacatcatcg tgaaacccga atttatattc
    13621 ttctaattct ggtacgccca tgttctcact cctcttcctt gtggtaatga tggaaagctg
    13681 cggtaccatc gttttccatt gcttttccta gtgctttcca ggcaagtgtc gcacatttga
    13741 tccgagcggg aaatttagct acaccaccaa gcatagcagc atcgcctaat tggtcgggat
    13801 ctttgacttc ttttccttgg acaagctgta gaaagtctac agttaaatgt tctgcttctt
    13861 ctattgattt tccgatgacg gcgtctgtca tcatcgaggc acttgccgta ctgatggagc
    13921 agccactgcc gtcaaaggcg atatttttga tgattccgtc ttttacatcg acttgcaatt
    13981 cgatcacatc tccacatgtg gggttgttca attcgatctt gcttgtcgag tcagtcaagc
    14041 ttccacgatg atgcggatgg gaagaatgat ccaagatcac ttgacggtat aaattgtcta
    14101 atttagatag tcccatgttg gaaaaactcc tttgttgcca tgattgcttc aactaaacgg
    14161 tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc gagcagtcgc tgatacttca
    14221 aggtacttca atagcggttg cgcacaatga tgacctgctc tgacggctac cccttccatg
    14281 tctaaagctg tcgcgacatc atggggatgt aacccatcca aattgaatgc aacgactcct
    14341 gtccgaaaat tcgggtcttg tggaccataa acagtcaatc catcaattgc ttgtaatttt
    14401 ggcaacacat aagcggctag ttcttcttca tatcgatgga tattgtccat accgagtttt
    14461 tgcaaataat cgattgcatg gcctaatgcg atagcgccgg cgatatttgg tgtccctgct
    14521 tcgaatttcc aaggtagttc tttccatgta ctgtcgtata attcgacaaa atcgatcatt
    14581 tcgccaccaa attcaacagg ttccatttct tctaaccatt ttcgtttacc atataaaaca
    14641 ccaatacctg ttggtccgca cattttgtgt ccgctgaaag caaagaaatc acagtctagt
    14701 gcttggacat caatggccat gtgcggggca gattgagcac catctactac gatgaccccg
    14761 ccgttttcat ggacaagttc agctaattct tgaacaggat tgaagacacc taagacattg
    14821 gatacatgag caatggagac gattttggtt ttgtccgtaa tctttttcgc tgcatcctcc
    14881 atatccagga agccttcttc tgttagttcg atatatttca agacagcacc tgttcgtttt
    14941 gccaactgct gccacgggat aacattagaa tgatgttcca tgtaagagag cactatctcg
    15001 tctcctgctt tgatatagcg gtcgccgaag gttctggcta cccaattcaa gcttgtcgtc
    15061 gtaccacgag taaacagcac ttctgctgtt tcttttgcat ggatgaacgc ccgtactttt
    15121 tctcttgctt gttcataatc atgggtcgcg cgttctgcta acgtatggac accgcgatga
    15181 acattggcgt tatcgtgttc gtaatagtac gctaatgcct ggagtacttg ttttggcttt
    15241 tgggtcgtcg ctgcattatc cagataaact aaaggttcat cattgacgtc ttgaaataag
    15301 ataggaaaat cattgcgtag ctttttagca tcgatcatcc attcaacttc ccttcgatta
    15361 cttcgactaa ttctttccga acttgttcta cagggattgc tgttaagaca gaaccgagga
    15421 atcctcgaat cactaagcgc tctgcttctt ctttatgcaa tcctctactc atcagatagt
    15481 acatttcttc tggatcaact cgtcctacac tggcagcatg tccagcagtt acttcgtttt
    15541 catcgatcaa aagaatagga ttcgcatcac cacgtgcttt atcagaaagc atcaagacac
    15601 gactttcttg ttgggcatct gcaccttttg cccctttcaa gatgtggccg ataccattaa
    15661 aggtcaatgt tcctttttct cggatcactc cgtgttgcaa aatatgcccg atcgtatgag
    15721 gggctttatt ggttactcgt gtatcgattc cttggatttg tctgcccgag ctgattgcca
    15781 cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc tgagtcaaag tcagcgatca
    15841 catgaccgtc attcatgaca ccaattgccc agtcgacaga agcatctcgt aaaatgtgcc
    15901 cacggcgatt catataagaa gtaatatttt ctcctaattg atctacggct gaatatttga
    15961 tttttgaacc agctttagcg atgacttcca cgatgatatt acctgatgat tttgctactt
    16021 gttctttggt tgtctggaaa cgttccagat aagaaaattc actatgttca tctgccacga
    16081 tcaacacatg cttgaagaag tgctcatcgg atgcgccatc ttgaatgaat aaggactcga
    16141 gtggttcatc gatcacgaca tttttaggca catacaggaa aataccgctg ttcataaaag
    16201 ctacatggag tgcagtcaat tggtcttcgt tcatgtttac tgcttttttc atataatatt
    16261 cttggactag ttctggatat tcgatcattg cagtgaacaa gtcggtaaaa atgactcctt
    16321 tttctgctaa ttccacaggc agttgttcaa aaatcgtcca tgaaccttgt tggacaagca
    16381 caggattgtc tttcattgca tcaaatgccg gaatcgtgcc tgtttcactg atggtttgtg
    16441 tttctttcac atcaaataac gaccagcgat ggaatttcac ccgatcgata tgcggcaatg
    16501 gcagttcatc tgctttcgct agtgcgtctt gacggaaggt tctcatccaa tcaggttcgc
    16561 ctttacttaa agaaaaatca tttaccgcgt caagataatc gagccagttt ttttctttca
    16621 ttcttgctcc cccttacgct tcttcttctt tatagtcgat tcctaattct ttactgatac
    16681 cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc attccctgtc atgactactc
    16741 ggccttccat catgatgtga acgacatcag gtgtgatata gttcaataat cgttgatagt
    16801 gggtgatgat caatgcaccg aaattgtctc cgcgcatttc atttactcct ttagcaacga
    16861 ctttcaaagc atcgatatcc agacctgaat cgatttcgtc taaaattgca aatgtaggtt
    16921 ctaacatcaa caattgcagg atctcattcc gtttcttttc tccgccagaa aaaccttcgt
    16981 tcagataacg ttcagccatt tcttctggca tatttagtaa agccattttt tcatctaact
    17041 ttttcaaaaa tgccataaca gaaattttgt cgttttcatc acgtttcgca ttgatcgctg
    17101 cacgcatgaa ttccgcattc gtgatacctg gtatctcact tggatattgc atagctaaaa
    17161 ataagccaag tcgcgcacgt tcatctactt ccaagtctaa aacatttttc ccgtcaaaca
    17221 aaatttcgcc ttctgttact tcatagtttg gattgcccat gatagcggca gataaggtag
    17281 attttcctgt gccgttaggt cccatgatgg catggatttc tccggttttc attgttaggt
    17341 ttacgccttt aaggatttct ttttcctcga tcgacacatg tagatttttt atttctaaga
    17401 cagacatgca ttataccccc aaattctctt tcgttctcac tcattttaga ctaattgaga
    17461 atgaagcgca atgaccttcc ttactaaaat caaatatctt ttatttatta ttaaacaatt
    17521 ttgatttcta aattagaata ggtataaaaa agatgagaaa taagccatta cagacttatt
    17581 tctcatcttt tttgttccat accttatttt gcatcagcct ttactggttt aacagagccg
    17641 ccccatttgt ctgtgatcca ttcttggact tctttgcggt gaagcacttc aatcagtttt
    17701 ttgattttgt cactgtcttc atcaccttta cgagtcgcaa taatattcac gtaaggagag
    17761 ttgtcctttt caagcaatac ggcatcttct aatggattca accctgcgcc gtaagcgaag
    17821 tttgcgttga ttgcaactag gtcaccttca tcattttggt acgtagaagc taaaagtgat
    17881 gggtcgatcg tatggttgaa cttcaagttc ttagggtttt cttcaatatc atcgaatgtt
    17941 gctgtttcca gatctactcc atctttgact ttgactaagc cagcatcttg tagaattgtg
    18001 atgatgcgtc cccagtctga ttcagaattt gaagtaatga ttgttgcacc atcttttaat
    18061 tcggaaacat ctttgacttt tttagagtac aagcccattg gttcgatgtg gacagcaccg
    18121 gcattgacaa agtcatatcc tttttctttg atttctttgt tcaaataagg aatatgctgg
    18181 aagtaattgg catcaatgtc gccttcttcc aaagattggt tcggtaatac atagtcatcg
    18241 aatttgacga tttcaagatc gattccttca tctttcaata atggtttcac atgttccaaa
    18301 atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg tcgtgtcgtc tgctttattt
    18361 gctgaatctt tgctgtcgct gttgttaccg catgctgcta atcctacagt cagcaaaagc
    18421 gctgctgcaa atccaaaaat tttctttttc atatcctaca tccccctata tcctattatc
    18481 ttttgtctgt tttcttagtt aagaaatcac cgattccttg aacgataaat acgattagta
    18541 aaataattaa agtcgcaacg aatgtcactg ttagattacc acgctggtag ccttcctgcc
    18601 aagcaagacc tccaagtcca cctgcgccga tcgcgccggc catcgcagta aatccgatca
    18661 tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg aatactttca ggtatcaaga
    18721 ccttccagat gatttcccag taacttgctc ccattgcatc tgctgcttcc agtacaccag
    18781 ggctgacttc acgaaaagca atctctacta gacgggcata aaatggtgct gcggataaaa
    18841 caagagctgg aatggctgct tgcgcaccta gcatagtgcc tacgattgct ttagtaaatg
    18901 gaatgatcaa aaccatcagg atcataaatg gcgttgaccg gaaaatatta ctgatgatcg
    18961 atacgattcc ataaatgatt cgagcatacg gtttgttttt gcgtccaata gagtaaagga
    19021 tcaatcccaa aactaatccg atgatcgtca ctaagaccat tgagattaat gtcatatata
    19081 gtgtatcaag gatagcctga ttcatcgttt ccgggttgat ttgtttaaaa tctagatatg
    19141 tttctgaaaa gcttttatcc acgatggatc acctcaattc ctactccttg ttctttgatg
    19201 aacacttctg cttgatctaa ttctttggtt tcaccagtca tcaagacagt caatgaacca
    19261 aaggaacctt ctttcacttg tttgatcttt ccatacacaa cgttgacatc gatgttatag
    19321 cgtcgaatcg cttgggaaat gactggctcg ttggcatttc tttcgctaaa ggtcaaagtt
    19381 gccaaacggc cgcttggatt ttctttgatg agttcagcta atagttctgt cgaatcagct
    19441 tctggttcga tgtcttgttg tacaaagcgt ttggttactt cttttttcgg ttgtctgaag
    19501 acagtcaata cgtctccttc ttctacgact ttccccaact ccatcacagc gactttgttg
    19561 cagattttgc ggatcacatt catttcatgt gtgatcaaga cgatggtcaa attcaatttc
    19621 ttattgatcg ctaacaataa gtctaatact tcttctgttg tttgcgggtc caatgcgcta
    19681 gtagcctcat cacaaagcag gatctcagga tcatttgcca atgctcgtgc gataccgacg
    19741 cgttgtttct gtccgccgga aagttgtgaa ggatatgctt gtccgcgtcc ttctaacccg
    19801 actaaacgca acagttcttc tgcccgttct ttacgtttgc tctttggtac gccagctaat
    19861 tccagcggca gctggatatt ttcaagtaca gttcttgacc ataacaggtt gaaatgctgg
    19921 aagatcatac cgattttctt tcggaatgta cgtaattctt tgttttttaa ttttgtaata
    19981 tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct caagtccatt caggagacgg
    20041 actaatgtac tttttcctgc gccagaatag ccgacgattc cataaatatc ccctttttca
    20101 acgtttagtg aaacgtcatt gacggcttgg atgtttccgt ttttcccatt gaatgtcttt
    20161 ttgacattcg ttagttggat taatgacatg ccattcacat ctttctctta taatttttca
    20221 aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac gaaagcttga acttccgtgt
    20281 taccaccttg atttaccgtt acttcgcaat aacagcctct tccagtactt ggacttagct
    20341 tctttccgta tactgtcgcg ctgtaacggg cactcccgta gtaagtttgc ttacgctcgc
    20401 ttactctgct ctaagaccat cttccatctc attttcgtta cccattttca gcaaacaggg
    20461 ctctcttcaa acgtccgtga catgtactct tcttttcaaa gctttctcgt ttcatatagg
    20521 attgcttatg attatagcaa ctggaagaat cagtgtcaac ttttttcaag tgattttctg
    20581 attttataat tcgtcaaatt cttttggatc aacatctttg aaacttaaga tccacgcatt
    20641 catttcatct gaatcattca agatattgat atcttcaccg attttatcat taacagaaga
    20701 aactgtaccc gttagcgggc tggcaaattc attgaccgct ttttccgctt caacttcgat
    20761 cagaggttca tcttttttta gcgattgtcc tgcttttggc agattggcaa acgtgatgtc
    20821 tccaagttct tcttgcgctt catttgtcaa accaacacag tattcttttc cattaaataa
    20881 gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc attttatatc agttctcctt
    20941 cataaatttt ttcattgaat ccaattgcaa caagctggtc ctctcgtaag atcagtggtc
    21001 gcttgatcag cataccatct gtcgataaga cttggctcgc ttttgaagcc gtaaactcgc
    21061 ctacttgatc tttcagtcca agttcccgat atttcatccc gcttgtgttg aacaggcgac
    21121 ggattggtac ttgactcgtt tctagccaag tctcaatcgt tgaggccttc ggtacttctt
    21181 tgatcatatc aacggtttga taactaacgc catgctggtc taaccacgct ttggcttttt
    21241 tgcatgttga acatttcggg taccagtaaa aggtgtacaa ttttcttacc tccttgaaga
    21301 tcttgcaggt gcttcatttt ggtatctcat cgataatatc aacccgatac cgatcatgtt
    21361 ccctaaaatc gaggatcctc cctgactaat gaaaggcaat gggatccctg ttaaaggaag
    21421 taatccgata tttgctccaa tattctcaaa gacatggaac agcagcatca tgatcaaacc
    21481 gcttgcgata taggcgtaaa attcattatt cgtatcgaaa cacacccgaa tcatacgata
    21541 gattaaaata aaatacaaga aaatgacgaa tgcaccaccg ataaagccga agttttcacc
    21601 gataacggta aaaatcatat cggactcacg aacaggcaca tagatattgc ttacgttgaa
    21661 tcctttacca aacaagccac cggtaccaat tgccaaaagt ccttgggccg gctgataact
    21721 cattcctgac gtatcatgga aagggtccag ccatgaatca atacgtgcaa actgataaga
    21781 tttgaatcca actttataaa gcaagtctct tccgccttca gtcgtaacca agaagatcgt
    21841 tcctgctcca acgactactg ccaagattac tacaggtgcg atgatgcgcc aagagattcc
    21901 agacatcagg aaaatcccgc caaagatcgc aagaaagaca agcatcgttc caaagtcatc
    21961 ttgggccatg atcaatgcta aaaccggtat tgtcacgatc aagagcttcc caatcagcca
    22021 aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta tgtttagtta cgatcagtgc
    22081 catcatcaag atgtacgcaa tcttcatcaa ctctgctggc tggaaagtaa acgcaccgaa
    22141 tcgaaaccag tttttagacc ctgttgaagc agctaaattc tgatcataga atttgagcaa
    22201 gagtcccatt accactaacc ccaaaccata aaggtaagga gtcagtttcc agatccattt
    22261 ggaattcaaa tgcatgatga caacgattgc tgccgctcca actaaatacc agacaacttg
    22321 catccctacg cctttcaaga ctgctggatt gttcggatca tggctaagtg ctacatataa
    22381 tgagagcaga ccaatcaaac ataacagaaa gactggtaaa atcacgccat aatcaatacg
    22441 attatcgttc gaagctttta attttttttc catgtttcat cccttttctt aaaaaccata
    22501 ctgctgatta ttatagcggt ccagttagat aatggaaagt ctttccatag acatttggga
    22561 aaaattaact ttccgtttgt gcagttgctt gagcaaatgc agtacaaaag cctactgctt
    22621 atctttagtg tatcatagaa ctttttttga tacatgcaat tatctaccag ctgagattct
    22681 cctcgctgtg agaagtttct gtgctttcat tgtatcgaag gatctcttcg gcaatctcta
    22741 gcagctgatc agaaaccaga ttggttcgtt ggatgaaata aaaatatcga tggaaattca
    22801 acggagtcat tggcacattt tctggaacag agcgcttaga caaaatcgag cgaccgattc
    22861 cttccttcag caacgcaaca attacatcgt tacttttcac gatcatcttc ttatcttgga
    22921 tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac accagagttt gcttctctta
    22981 caagccacaa tttgcttttt gggtccccag cgatgactag ctggtcttcc atcaaagagt
    23041 atctttgtac gccggaagtc tctaatggct tttcgataaa accaaaatcg gattcatgtt
    23101 tcgctacttg ttctaatgat tcatgagaat tcgtgatttc gatctcaaaa tgcactttag
    23161 gaaacctttt gatcagatgt ttcatcaact gaggcaaaag atagacagca aaggtgtggg
    23221 aagcaacgat ccgacatact tcttcttcat tttgttcatt gagcaatttt tgctgagttt
    23281 cttcccattc ttctaacaaa accagtgtcc gttcatataa taagtcggct tgcttagttg
    23341 gaagcatctc ttttcttccg ttacgttgaa aaagatcgat attcaattcc cgctccaact
    23401 gtttgatttg attcgagact gctggttgag ataaaaacag tacttctgct gctctggaaa
    23461 aatttcttgt ttcatatact gctcgaaaag tcgttaataa cttaaacatg gcaccacttc
    23521 cataactttt tatgataatt actataaaaa ctatttgatt taaatataca aaaaaccatc
    23581 tataatagaa atgtacaaca aaacagttgc tgatccaact agtaacttag atgaagacaa
    23641 tcagctgtct gcttgctcgt aatcatacat tcctattcat tttacaatgt attcgttcat
    23701 cttggtacta aaatctatta aataaaaagg agaatattat atatgaagaa ctttttgaaa
    23761 attattccga tcccgatctg cgggttgatc cttgggttag cttcccttgg aaatctgttt
    23821 aaagactatc accatgttgc tttaggcaat ctaactggcg gtatctctat gattcttatg
    23881 atattcattt ttgttaaatt gtttatctta ttcgaacaca cgaaacaaac gttgcatgat
    23941 ccaatcatcg cctccgtttc accaactttt acgatgtcac tcatggttat ttgtacttat
    24001 tttgtttcat atgaaagtat tgcaccaatt gtcaaattca tttggctgtg cgctgttatc
    24061 ttccaagtga tccttgtctt ttatttcaat tatcaccatg ttatcaaagc tgatctttca
    24121 attgaagcaa tttatccaag ctggtttatc atttacgttg gatttggggt catcactgta
    24181 actgccggga acttcttccc acttattgga aaaattttct tttggatttc attgatctgc
    24241 tatgcgttct tattgccgat catcatcaaa cgtgtctttt tcgtaagaaa tatggctcat
    24301 ccgacacgtc cgctgattac gatcgttgca gcacctggtt ctctttgcct aacaggttat
    24361 cttaaaaact ttgctgaacc taatttttat cttgtcgtag cattgttcgc tttatctcaa
    24421 gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt tcaagcttcg tttttatcca
    24481 agctatgcag cctttacatt cccgcttgtt atctctgcaa cagctatatt tactactgtt
    24541 cattacttca acagtttagg tatctatagt agcgtattgg atttcttaaa agttgcggaa
    24601 tgtctgtttg caagtgtagt tgtcttctac gttcttgctc attacgtacg tttcttaatt
    24661 aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa tcgcttaata atcgattcaa
    24721 taggttgtga aaaagctgtt ctgcatcaag taataagaac ggccaaacaa aaacagctgt
    24781 tcatgttttt gtactggttg ggcttattac cgcagatgca agcttttgaa caccgtttac
    24841 caagaggtcg taa
//
LOCUS       DACJGB010000042        24775 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.042, whole genome shotgun sequence.
ACCESSION   DACJGB010000042 DACJGB010000000
VERSION     DACJGB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.042"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(43..2187)
                     /locus_tag="IYI34_001735"
     CDS             complement(43..2187)
                     /locus_tag="IYI34_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR0559604.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2544..3917)
                     /locus_tag="IYI34_001736"
     CDS             complement(2544..3917)
                     /locus_tag="IYI34_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0559605.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="IYI34_001737"
     CDS             complement(3917..4312)
                     /gene="rbsD"
                     /locus_tag="IYI34_001737"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAR0559606.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="IYI34_001738"
     CDS             complement(4340..5257)
                     /gene="rbsK"
                     /locus_tag="IYI34_001738"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAR0559607.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5365..6378)
                     /locus_tag="IYI34_001739"
     CDS             complement(5365..6378)
                     /locus_tag="IYI34_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0559608.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6459..7058)
                     /locus_tag="IYI34_001740"
     CDS             complement(6459..7058)
                     /locus_tag="IYI34_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR0559609.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7244..7945
                     /locus_tag="IYI34_001741"
     CDS             7244..7945
                     /locus_tag="IYI34_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559610.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7955..10666
                     /locus_tag="IYI34_001742"
     CDS             7955..10666
                     /locus_tag="IYI34_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAR0559611.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11117..12745
                     /gene="treC"
                     /locus_tag="IYI34_001743"
     CDS             11117..12745
                     /gene="treC"
                     /locus_tag="IYI34_001743"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAR0559612.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12805..13986)
                     /locus_tag="IYI34_001744"
     CDS             complement(12805..13986)
                     /locus_tag="IYI34_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR0559613.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14238..15761
                     /locus_tag="IYI34_001745"
     CDS             14238..15761
                     /locus_tag="IYI34_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAR0559614.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15830..16558)
                     /locus_tag="IYI34_001746"
     CDS             complement(15830..16558)
                     /locus_tag="IYI34_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0559615.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16571..17851)
                     /locus_tag="IYI34_001747"
     CDS             complement(16571..17851)
                     /locus_tag="IYI34_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAR0559616.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17992..18594)
                     /locus_tag="IYI34_001748"
     CDS             complement(17992..18594)
                     /locus_tag="IYI34_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAR0559617.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18839..19045
                     /locus_tag="IYI34_001749"
     CDS             18839..19045
                     /locus_tag="IYI34_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559618.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19357..20076
                     /locus_tag="IYI34_001750"
     CDS             19357..20076
                     /locus_tag="IYI34_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAR0559619.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20157..20840)
                     /locus_tag="IYI34_001751"
     CDS             complement(20157..20840)
                     /locus_tag="IYI34_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAR0559620.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21058..23244
                     /locus_tag="IYI34_001752"
     CDS             21058..23244
                     /locus_tag="IYI34_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAR0559621.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23590..24000
                     /locus_tag="IYI34_001753"
     CDS             23590..24000
                     /locus_tag="IYI34_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAR0559622.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            24014..24370
                     /locus_tag="IYI34_001754"
     CDS             24014..24370
                     /locus_tag="IYI34_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAR0559623.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8282 a         4403 c         4837 g         7253 t
ORIGIN      
        1 aaaagggagt gtgacagtgt cacatcccct ttttaattta ttctatttct ttaaaggcag
       61 atcaagccag aatgatttgt ctaaacttaa caaggttagt acaggaacag ttgcagcttt
      121 aactgtacga gtagttgttt ctggtatctg tcttgccgta aaattgttcc acaaatccca
      181 gattgaagcg acacggaaga tggaagaagc gttgttgacg cgttttatcg ttgaaaattc
      241 taatcctgtg attggcacgc catctgttgt ccatttgatt ggcacatcga atcgcttaga
      301 catattgagg atttcagcgt ttgcttcttc agttgttgct gcttcttttt tcggttcatc
      361 atattttgtc aggtcgtacg cttcgatgat cgcgttcaat ttttgtgaat attggcgatc
      421 tgtagcataa cgtccttcca aatatttggt tgcttctttg tagtcttttg tttccgactt
      481 ccaagttggt ttgtagaaat caggattgcc agtcagtcca tttttgatca agtcggcata
      541 gtcttccaaa ctttccttgt aagaaggata tttacggaat tgtgcgcgga tttggtagct
      601 ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg ctgcttcctt tatatgatcc
      661 tttgatacca aaaaggttat gatttggttc gcttgataat aagctgtttc cagatccact
      721 ttcaaggata gcttgagcca tcatgactga tgcgtagatt ccttcttttt gcccgatttc
      781 ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt gtttggtttt gactgaaatg
      841 gaacgagcca tctaatccat ctaaaataga tgagccgcct gtcagcgaac ctgaagcagc
      901 aggtaaacct gctaagttta ctcggacagc aaaatcaggc gtgaaatctg aaatagaagt
      961 tactcgaact ttatctcctg gttgaggcgc atggataaat tgtccgttcc caatatagat
     1021 acctacatgg tgagtaacac cggaagaagg tccccagaag acgaggtcgc caggttgggc
     1081 ctgacttact ggaatctgcg tacctgcata ttgctgttcg cctgtccagc caccgatata
     1141 atgaccggtt gctttcatat atacgtaata agtcaaacca gaacagtcaa aagtatttgg
     1201 accttttgct ccccatacat atggttttcc aagatgcttg aatgcttctc gaacgattgc
     1261 ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca attggtgatt ttacaacaaa
     1321 atcagaagaa gcagaagatg gattctctat accgctttga tttgctgtat ttggacgaga
     1381 tgaagcattg ttcgttccgc cattcgatga accattgttt ggatggtttg gttctctctg
     1441 agtatttcca ttgttattcg atccagatgg gtttgtttga ttcgaagaat tcccgctgtt
     1501 cgaattattg ttattattgt ttgaattatt tgaaccatta ttattgttgt tattacccac
     1561 atctggtttg tttggcgttt ccggatctgg tgtactaggg acttcttcac taggatcagt
     1621 tggtttctct gttgaagtat ccggtccagc tttaaattct gcagtaatag ataaattgtt
     1681 cgtacctact gtaaaggtct ttgcatcata atttacttct ttggcaccat taacagcgac
     1741 tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa gttaccttcg tttccgctaa
     1801 taaaccttct accgttccac tttccgtaat atccaaaggt tgctgttcag gggaatccgc
     1861 tgtcgaagca gtcagttgga tcttaccaat caaagaagga gcaattgtaa gggaatatgt
     1921 ctcggctggc ttttcatttt ctgatggatc agccggatca atttcagttg gctcggtcga
     1981 tcctgttgtt gagttagttg ttccagttgg gtcttctgta aaagaatccg ttgtttcact
     2041 agtagctgag ctggatgtag tcgtatctgt tgtgactgtt tgatcagcta tttgattatc
     2101 tacggcaaat acggtattgg tcgacaaaac agacggagca atcagccaag tagcagctag
     2161 caaacgtatc gtttttttat ttttcataat aaactagtcc ttttcctttc attgattttc
     2221 tgtcttattc agcattcatt agacattttt tggaacagat aatcgtgtcg aaaagctttt
     2281 tcttttcata aatctgtctg tgtattctaa ataaaacaat ttgtaaataa gcaacgagca
     2341 ttcattcgtt tttctttgac gttcatttta ttgcatagac cacctttgtt tggggataaa
     2401 caagtgggga taaaaatggt tatgcatatg taaattatac caaagtttgg aaagtatcac
     2461 aaaaatttat aaaaataatg atcctaaaaa gaaaaagact gaaatatgtg ataaacatct
     2521 catacttcag tctttttcat taactattca taagacatta ggtcttcatc aaacccgcta
     2581 acgagagtag tgatcattgc tacgactaga ctaacagcca gtcctgctaa gtaccagaaa
     2641 aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac caaatacaaa agcgatcgct
     2701 ttcacttggg caaatcccat tacagctcca ccagcagctg caccaatgca agcagcaaca
     2761 aatggttttt tcaatgggat cgcaattccg taaacagttg gttcggtcac tcccgcaagt
     2821 aaaatcgaag caaaagcaga accggagatc gaacgggttt ttgaacgttt acttcgcaga
     2881 aatacaccta aagctgcacc agccattcca aaattggctg ctcctgtcaa cggaacgatt
     2941 cggtcgaacc cataagcaga gatattactg atcatgacag ggtttacagc ccattggata
     3001 ccaaatatta ccaaaatatt ccaaccacca gcgatgattg cgccactgat cggtccgtta
     3061 atatccatca tccaagtaat tgcggcagct aaaccttcgc caaggtaaac acccgcaggc
     3121 ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg aaagtaaagg tacaaatact
     3181 aattgcatat tttcgcgaat atattttttg aggaattttt caacaaaaga ataaaagaaa
     3241 atacctaaaa tcgccggcat gacagtagac gtatactgca tcagtactac aggaatcccc
     3301 agaaaatcag tagctgcttt gccactgtct gaaagtaaag aggtcagatt cggttcaatc
     3361 agtgctgcag caatgacagc cgcaatatat ccattcactt taaacgtttt ggcagcagaa
     3421 aaagcaagaa agattggcag aaaataaaag acggcattcg acgcagcaga caaaatatga
     3481 taagttccgc ttgtttcact aagccagcca atcgtagtcg ctaaaacaac cagacctttt
     3541 aatataccag aaccggcaaa cagcggaatg atcggcgtaa agatttccga tatcaaacca
     3601 aatagttttt cgatcggatt tagcttttct ttcggagctg aatgatcgat ttgacgcgtc
     3661 aattccttgt aagcaggagc caatccctga tccatgatta tttctgcata tctatcgagt
     3721 aaaatgaaat acttaatgga agggatttgc tgttttaatt tttctgcatt ggctactggc
     3781 tgttttttca atttgatacg aagccgagtc gcacatggaa ggacagaatc aatattgtct
     3841 tttcctccca actgttcaat cagcatatga atctgctcgg acagtaccat ctgttcagga
     3901 ttcattgtcg tttccattag aagaccactc cactcactaa tataatattg gcgtaaggtg
     3961 tcatttcgcc tgtacgaata aatgcatgac agttattcaa ctcttttttc atttcttcat
     4021 gtgggataaa gctaatcggt gtatctggta acaataattt gattttttcc aatatttccg
     4081 ggttcattgt ctttatttct tccgccaatt caatcgattg tacctcaagt tcttctaaaa
     4141 cgttttctaa aacagataaa aaacttggaa gaccgtttga gacagccaaa tctatttttt
     4201 cagtaccaaa ggggacaggc atgcctgcat ccccgatact taatttatca aagtgcccca
     4261 tttgtgcgat tactcgtgaa atatctgaat tgattacttt tccttttttc atgtttgttt
     4321 cttcctctca ttgttaattt tagactgctg attgttctaa agcttcgcgg taagggatag
     4381 aaggttgcgc accataagac tgcacagcaa cagaagaggc tagatttcca taaaggatcg
     4441 cttctttcaa gttactaagg tctggctgaa gtgtgctgct taatgcgcca ataaatgtat
     4501 cacctgcggc tgttgtgtca acggcatcca ctttaaaagc agggacgata ccgtgttctt
     4561 tttctgtatg atagaaagcg ccggcactgc ctagtgtaat gattactgtt ccaataccta
     4621 attcatggag tttttcggct gctgcgacca aactgttatg atctgtcact ctgattcctg
     4681 taatgatttc tgtttctgtt tcatttggta taatgatatc tgtcttttca agtagttcag
     4741 taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt tgtttttcct gcgtcttttg
     4801 caatagaaaa agctaggatc gtcgcatcta agctactttc aaactgagca ataataaagt
     4861 cactgtttgt aatcagttgc ttgtttttta ggacttcttg ttcatggaaa gcattatttg
     4921 cccctgcatg gatcaagata ctattttctc ctgctgcgtc taccataatc attgcttgtc
     4981 cggttgtggc gccttggatt tcagctatcc cagatgtatc gatgttttct tttgtcagta
     5041 gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc tccaataaaa gatgtccgtg
     5101 cgcctgaacg tttggctgcc actgcttggt tggcaccttt acctccgcca gcgtgaaaaa
     5161 tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt ggttaaacga agggtagtgt
     5221 ccatattaat acttccaatt actgtaaccg tgttcatttt acaaacctcc aactatctca
     5281 tttattttga tgggaaaatt attttttaaa acgttttagt aaaacgtttt aatagttcga
     5341 taaaaatata gcacaccttg aaagctattt caagggtgca gttgaaaatc ttttttctaa
     5401 tcgtacaggt aatctttttt cttcccattc tttttctggg aattggatcc gatctaacaa
     5461 taacttggca gatgtttgtc ccaattcgaa gattggttgt gcgatcgttg tcagtttcgg
     5521 cttgatatac tcgcacatat ctatattatc atagccaata atgctgtagt cttcagggat
     5581 gctttttcca gcttcctcta atccgcgata taatccaaaa gctaattcat catttaaagc
     5641 aaacacacca gttgcagcag aatcaagaag ttctgctgtg atctggtagc caccttgttt
     5701 tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg atttggtaaa catcaagagc
     5761 agatttaaac ccctcaatcc gagcgtggac attttccggt ggattttcag gatagacaag
     5821 ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc gctaggtaac caccgcgaaa
     5881 atcgtctgtt cgtacggcat cactaaatcc ttcagatttt ttctgatcca aaacaatgaa
     5941 gggccggcct tgttttttca aattttcgtt gattgcatct gtagagacag cagaagtcgc
     6001 gataatgaat ccatccacac ctcgacgtgt gagctcggct agatattcga tttctttttg
     6061 atgatcagaa tcagcattac ataatatcgt gacgaaattc tgcttataca agatatcctc
     6121 aattcctcgc atcagtgtac tgaagaatgg attcgtgata tcaggaacga gtacaccaat
     6181 tgttttcgtt tccttggtaa tcatttgtct agcaaaatag tcaggctgat agcctaattc
     6241 ttctttggca gctaaaactt tctgaacagt tttcggactg aatttctccg tatttccatt
     6301 taatatcaag gagacagttg tgattgaaac gccggaaagt ttagcgactt ctttgattgt
     6361 gatctttttt ttcaccatgt tcatactcct atctaaatcc tgtggttatt ttcccatgaa
     6421 atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt atttgctttg ttggatagct
     6481 tcgattattt gtttcgttga gcgtactcgt gcagttttcg gaaatatctt ttttatgaac
     6541 aattgatggg attctttgtc acgatcactg caagcatctt cagcaagcac gatatggtac
     6601 ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt ttgttgtcgc tacaccgctt
     6661 aaaatgatag tttcgattcc acgccggcga agttgaagat caagagaagt accaaaaaaa
     6721 gcacttggat tgtattttgt gatagtcaac atattatctg tatctttcaa agaatccttc
     6781 aaaagtaaat cagtgaattc tggaggtaca gaagcagtca ttccacccat atcagtttgt
     6841 tgactcaaat aagggaaagt ggagatgtct acgttcacta aaactttcaa ggcagcggta
     6901 cgtttcaatg ttcttactaa caaatcattg tttgtcagaa tagatgctgc agtgtgtgga
     6961 gagagtgtac cgttatttac gatctctttt tgcatgtcga tcgcgacaaa agcagttttt
     7021 gataaatcaa aaatcatatt ttcctttaaa tcattcatga tgttccttct ttcaaataac
     7081 aatgtttttt atgtaagtaa atcataaatc gtttatttgt caactaaatt aaaggattcc
     7141 tttctttagt ctagagaaat tcagtggtaa aataaataag tgtgagaaaa cgcattcgat
     7201 gcatagatat agcgagaaat atcgtgaaaa tggaggaatc agagtgaagt atgtcgaagt
     7261 aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc attacagcaa atgacgatat
     7321 ttctttttca attgaaaaag gcgaattagt gattatactt ggtccaagtg gagcaggaaa
     7381 gtcaacgatt cttaatattt taggtgggat ggatacacct gatgaaggtc agatcattat
     7441 tgatgacaca gatattgcgc aattttcaga taaacagctt actgcttacc gacgaacaga
     7501 tgtagggttc gttttccaat tctacaatct cgttcctaat ctaacagcaa aagaaaatgt
     7561 ggaattggca acggaagttt ctcctgatgc gcttgatcct gtagaggtat tgaggcaagt
     7621 gggcctagct catcgattga ataactttcc gtcccaatta tcaggaggcg aacagcagag
     7681 ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt cttttatgtg atgaaccgac
     7741 aggcgcgctg gattttgaaa cgggcaagca agtattgaaa ttattgcaaa acgctagcag
     7801 acaacacggc aataccgttt tgatcattac acataattct gctttagcgc ccatcgctga
     7861 tagagtcatt cacattaatg atgctaaagt aaggtctgtt gaattaaatg atcatccttc
     7921 atccattgat gagattgtat ggtaggggga ttttatgaaa acaagaacat atcttaaagc
     7981 aggttttcgc gaaattcggc aatctaaagg acgattcatt gcaatcatcc ttattgtcat
     8041 gctaggaaca cttttatttg taggtgtcaa aactgctgga cctgtcatgc aaaaaacgat
     8101 ggatcactat gttggtacag caggactttc tgatttgcaa atcgtttcta ctggtggctt
     8161 gacacaaaaa gatattgctc aagcagaaaa gatcccagat gcaaaagtag aaacaggaaa
     8221 acagatctat tattccaata ctggcaaaaa cgaagtgatt caaatatttt cttataacaa
     8281 aaataacaag cagaacaagc tacaattaac tgatggaaaa ctcccggaaa aaccaaacca
     8341 gcttgtattg gatgaaaaag cggaagatga agggtataaa atcggtgata tttatcagat
     8401 tgattcagat gaattgaaag aaaaagagta taccatcacg ggctttgtcc gttctccatt
     8461 gttcatcaac aatttggaga gaggatacgc gaacgtcgga aatggtagcg tcgattattt
     8521 tgtctattta cctgaatccg tttttaaaac agatatttat tctgtcttat atcttgattt
     8581 tacaaacgta aagaatttga atacgtacag caaagcttac aaagataaaa tggagcaaaa
     8641 tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa gaacgcttag aagagctgaa
     8701 aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa atagcggatg gcaaagcaca
     8761 aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa cagttagcgc aacagtcagc
     8821 aactatccag cagcttcctg aagcgcaacg aacaatcgct gttcaaacat tatctcaaca
     8881 agaagctcaa cttgctgaac aagaaaggca attagttgag aaagaacgtg aactggcctc
     8941 tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa aatttaacta aaccatcata
     9001 cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa tttggtgatc tgtctgatcg
     9061 tatcgctgcg attgccaatg tttttcctgt tttctttttc tttattgccg cattgatcac
     9121 ctttacgaca atgacacgta tggtagaaga aaaccgcagg gaaatcggga cattaaaggc
     9181 gttaggttat acgaaagttg aaatcgcaaa aaaatatgca atctatgcaa gtctagcctc
     9241 agggataggt attgttttag gaacgatatt aggaacgaat cttttgccaa ggatcatttt
     9301 tgagctgtct aacgagcggt atgatattgg gagtgcgctt attttttatg actggccgcc
     9361 gattttacaa gcagctgccg cattcttcat tgccgcattt ggcgctgcaa tgatcgtttt
     9421 attcaaagat ttaagagaac ggcctgctgc attgcttcaa ccgaaagcac cgaagccagg
     9481 gaaacgcatt tttcttgaat acatcactcc gctgtggtca cgccttagct ttaatcaaaa
     9541 aatcagttac cgaaatctct tccgatataa gtcaagaatg cttatggcga ttatcggaat
     9601 tgctggatgt gccgggctga tggttgcagg cgtgggattg aaagattctc taagttctgt
     9661 ttcagccaaa caatttggac cgatcattga ctatcaagca atcgttacat tggattcatc
     9721 cacggaagat agtcgtcaaa aagcagcgga tatgctgcag aagcaagaaa aagtaaatga
     9781 gatcctagct attcatactc aaaccgtaga attgagaaaa aaaggacatg cttcgcaaag
     9841 tctgacagtg atcgtaccaa gtaaaacagc tgatttggat cgttttatcc atttaactga
     9901 tcttgaagag aacccacgct ccttgcctaa aacaggaatc gctattacgc aaaaagtagc
     9961 agaactattt gacttatcta caggagatca tatttcgctt tatgatgaag atcaaaaaga
    10021 atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta ggtagcttta tttatatgtc
    10081 ccctgaatat tatgcttcag ttactgatca atcgatgatg gataatgcat ttttgctgcg
    10141 tacagataag atgacaacaa aagaagaaga aatgctttcc gagaaactgc ttgccacaga
    10201 cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa acgcaagaag aatcaatggc
    10261 gaacttagac tcggttgtct taatctttgt cgtattatca ggactgctgg cttttatcgt
    10321 cttgtataat ctaactaata tcaatatttc cgagcgtgta cgcgaattat ctacaataaa
    10381 agtccttgga ttttttgata aagaagtcac gatgtacatt gttcgagaaa atatcatctt
    10441 tactctttta ggaatcatcg gaggatttgg tgtaggatat atattgactg acttcatctt
    10501 gcaacaagcg tctatggaga atgtcatctt tccattagtg atcacatggt gggcttatgt
    10561 cttatcagca ggattaacta tcgtatttac ggttattgtg atgattgtta cccatttcaa
    10621 attaaagcat atcgatatga ttgatgcgtt gaaatcgaat gaataaaaac agaggttgtg
    10681 aaatcagcgg tttgacctga gtattagaga aacaaacggc aaaatagttt ttcatatttt
    10741 gccgtttttt gagctaatac cgaaggacag ctgattgaac accatgaatc agaggttgta
    10801 aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata aaattagctc ctcatgtttt
    10861 acatttgaga agactgattg ccgtagataa aagcttttga acaccattta tagaagaaac
    10921 aagaatattt tgctatttta aacaagagga tagacaaata catgtctgat ctcttgtttt
    10981 ttattgggca acagacctct tagaatgtat aaagataaat tttacaaacg ctttcggttc
    11041 agttataata ataaatgata acttgtatat ataagttgtg gtaggtaata aatcagttga
    11101 atggaggtaa atatccatga catttaaaga caaagtcatt taccaaatct atcccaaatc
    11161 ttacaaagac agtaatggtg atggaattgg agatttaaaa ggaatcaaag aaaaactccc
    11221 gtatctgaat gatctaggca tcgacatgat ttggctaaac cctatttatc caagccctca
    11281 aaaagacaac ggttatgata tctctgatta cacagccatc gatcctatct ttggtacgat
    11341 ggaggaattc gaacagctaa tagccgaagc aaagaaatac cggatagaaa tcatgctcga
    11401 tatggtgctg aatcacgttt cgacagaaca tgaatggttc aaaaaagcac tagcagggga
    11461 ccagcattat caggacttct tcattttaag agaagagcca acggattggg tatcaaaatt
    11521 tggtggaagt gcttggtcgc catttggaga tacggggaaa tattatttgc acttatatga
    11581 tcggacacaa gctgacttga actggcggaa ccaagcagta agagaggaac tattcaaagt
    11641 tgttcgtttt tggatggaaa aaggggtaaa aggattccgt ttcgatgtta tcaatgttat
    11701 cggtaaagac gaacaattga aaaataataa agaaaatgat ggcaaacctg aatatacgga
    11761 caaaccaatc acccatacgt atctaaaaga attgaaccaa gcaacgtttg ggacggattc
    11821 cactatcatc acagtcggtg aaatgagctc tacgacgatc gaaaattgta tcttgtatac
    11881 agaacctagt agagaagaat tatcaatggt ctttaatttc catcatttga aagtcgatta
    11941 tgaaaatggg aataaatggt ctactcctcc atttgatttt gcagaattaa agcgactatt
    12001 tcatacatgg ggagaaaaga tgagtgaggg taacggctgg aatgctctat tttttaataa
    12061 tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc gatcagtatc gaaaacaagg
    12121 agcagagatg ctagcaacca tgatccattt gaaccgtggg acgccctata tttatatggg
    12181 agaagaaatc ggcatgatcg acccagacta ttcatcgatt tccgactata ttgacgtaga
    12241 aagtttgaat gcttataagt tactccttga acaaggattc acagaaaaag aagcgtttcg
    12301 tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc atgcaatggt cagacacaga
    12361 acaagcagga tttactacag ggaaaccttg gttaaaagtt ggaggcaagc tagaagagat
    12421 taatgtagaa aaagaactat cttctgaaga ttctattttg tcctattaca agaaattgat
    12481 tcggctgaga aaaacatatc caatcgttgc acaaggagat tatcatgctt atgaagcaaa
    12541 tcatccggaa gtttatggct ttcttcgcca atttgaagga cagcagctgc ttgtattaac
    12601 aaatttctat gataaagata caaggatcgc catcccagaa ggattcacag aagccaatat
    12661 tcttatcgat aattattccg atactgttct tcatcaaagt ctggagctaa aaccatacca
    12721 ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa cgtcgttcct acgagctatt
    12781 ctcgtaagat cgacgttttg tgttttattt aaaataatct tcttcagaaa atgaagagtt
    12841 ttcatactct cctttatcgc cataaaattt attgtagcgt tgtttgaatt tacggaaaat
    12901 aaaagtaacg agtaatttga ctaacgtata gataggaata ccaaaaataa cgcccacaat
    12961 tcccattaga tcacccatga caagtaaaac gattaagatc gtaatcggat ggatctgtaa
    13021 acgatcaccc attactcgag gaatcaccaa gtcaccgtgg agaagttgta cggcaaacca
    13081 gacaatgaca aatttgacag ccataaaagt agagtcttgg aatgcaatga tcaaacctgg
    13141 aaagaaggcg ataaacggcc caatatatgg aatgatacat aacaacccag cagaaatagc
    13201 taaaacacta gcaaattcta atccgatcag taagaaacaa acccagtaga ctgctccaag
    13261 gataaatgaa gcaatgattt gcccctttag aaatgaaccc aattgttgat tagcaatctt
    13321 tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca atcgctaaaa ctgactgata
    13381 aaatttttga ggatctctca aaaggaaaaa agcgacaatt ggaccagtaa ataaagtaat
    13441 aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt tgtgcgccac tttcccaata
    13501 atttccaata aatccagcta tatcagacgt cacgtcgtca atagaagcga aaaattgatt
    13561 aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg agactctctt gaaaatcttg
    13621 aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt tcttcaagaa aaggaataag
    13681 ccatatgctt ccaagcccca ataaaaataa aataatcaca taaacgataa tgactgacaa
    13741 gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt tccaaaagat aatacaagat
    13801 cagtgcaaac aataatgggg gcaagatagt agcgataatg attccgacag gcttgaatat
    13861 aaacgagatc tgatggaaaa aataaatgat cagcgcaagt aagataacta ttcctaaaac
    13921 atagtaagag gtttttcctc ctaaaaagcg gatcaaacga ttcgttgtat ctgtttcttt
    13981 tttcactctt tttcctcctt tatctataac ttcattatag acaaaagcgg gtataaaagg
    14041 aaaatgatag aggttgattg caaaaatatc gtcaatcatt catataaact ttctcatcaa
    14101 ttaaaataag aaaacgcttg acagttcttt taaccatatg ataaattaaa tcctgttgct
    14161 aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac attcaaatga aagaagatga
    14221 gaagaggagt gtcataaatg aaacgattga taggtatatt ggccatgctg gttttagcag
    14281 gtatgttaac tgcttgcggg gcaagtggga aagccgagga cagtaaagaa aaattgagcg
    14341 tgatgacgac cttttatccc atgtatgatt ttacaaaagc aatcgtcggg gatgaaggag
    14401 aagtagagtt attgattcca gcagggactg attcccacga ttatgaacct tctgctaaag
    14461 acatggcaaa gatccaagat acagatatct ttgtttataa tgatgaaaac atggaaacat
    14521 gggtccccgc aatccaaaaa acattacaag aaggcaatgt ccatacgata aaagcgacag
    14581 aaggtatgct tttacttccg ggaagtgaag aaggacatga tcatgaccat gaacacgggg
    14641 aagaaggcca tacacatgag ctagacccac atgtatggct agctccaagt ttagcaatca
    14701 agcaggtagc aaacatacgt gatcagctga ttgaagctta cccagaaaaa caggaagtat
    14761 ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc actccatcag ctttatcaag
    14821 aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca acatgctgca ttcaactatc
    14881 tagcgttaga atatggattg aatcaagtat cgattgctgg tctttcttct agtgaagaac
    14941 cgtctgcggc gagaattgct gaactgaaac actttgttaa agaacatgga atcaactata
    15001 tttattttga agaaaacgcc aaagatagta ttgccagaac gttagcaaac gaagcaggtg
    15061 tatctttaga agtattgaat cctttggaag gattgacgaa tgaacaaata gaaaatggag
    15121 aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt gaaaaaaacg acagagacag
    15181 aaaacccgct agaagatact ttgaccccca aaaaagaaaa gagcgtctac aacggttatt
    15241 ttgaagacga ggatgtacaa gatcgctctc tttcagattg ggcaggaact tggcaaacag
    15301 tagatacgta tgtagaagat gggacttttg atcctgtttt tgaatataaa gaaaaattga
    15361 atcaggataa gacagcacaa gaatataaag aatattatac gaagggctat caaacagata
    15421 ttgcttcaat caagatcagt gagaaagaga tggcatttac gtttaagaat ggaacaacga
    15481 aacgttcaaa ttatcgttat gtagggaaag aaatcttgac ctacgaggca ggaaaccgtg
    15541 gcgtaaggta tctattcgaa gcagaagata aggaaagtgg cgcttttaga tatattcaat
    15601 tcagtgatca cacgattgca ccggctgact ccgaacattt tcatttatat gcaggaaatg
    15661 aaagtcaaga agtattgctg aaagaaatgg agaattggcc aacattttat ccagatacaa
    15721 tggatggacg tgaaattgca caggaaatgc ttttgcactg ataggcaaaa aagaaccgaa
    15781 gggtttggat cggctggtca tttagtcggt ccaaactatt cggttctttt tatgaaatca
    15841 atttctttaa tccacggata gaggctggga tggtctcgcc gtttgccaac gtgacttctc
    15901 tggttttttt attaatcgac cgcacatttt gtgtattgat aacataggat ttatgacaac
    15961 ggataaaatt ccgatgcatt ttctcgattt ctttgatctt ctcatagaat tgtaattggg
    16021 catgagtcaa atggacttcc agtttatgtg gcgctcctag cgaagtagaa aaatattgga
    16081 tgtctttgat cggtaaaaat ttgatctcat cattaaactc tattttgaag atatcttttt
    16141 ggatttggga catttgaaaa tcttcgaaaa ttttgctcag gatatgctcg acattttctt
    16201 ttaaatcata aagatcttct ttgacgatat aatcagtcgg ttcgatgttg ctttttaaga
    16261 ttttcatcga catttccgta tgggaagtca catagattat ttttcctaaa ggatctaatt
    16321 cacggatcaa tttaccgatc gctacaccat ccatttcact gtcttctagt tcgatatcta
    16381 aaaagtaaat accaaaagaa gaagttctga tcttggctgc attgagaagt tcttccggat
    16441 cagaggtaga aatatggaca taactgtcta agttttcaat catgattcta tttttgatca
    16501 ccgttgataa aacttctaat tgttttggat cgtcttcaca aatataaatg ggaatcatat
    16561 cattactcct ttattttatt tgtatataaa tcgtttgaga aaaacgctgg ttttcaattg
    16621 atgtttcaag gaaaagataa ggattggtct ttgtcaattc atccaagata aacaagccaa
    16681 ggccttcgtt ttttggattt gtttttgttg agaacccttt ttgtttcaac tggtttaacg
    16741 gtaatgtatt tgttgttgta ttgatcacac gaatcatcac ggaatgattc ttcttgagta
    16801 tggtcaattc gatttttttc ttctttgcag cactagctcc ttcaatcgca ttgtccaaga
    16861 taataccaag catacgtaca aggtttaccg tatgtttcgt atccagttcg attggctcag
    16921 gaaaatgaag ctggacgatc aacccttttt cctgtgccat catgagtttt aatgacagta
    16981 aactacggat ctcaggaact ttcatattat ccaacatact tagattggcc ggaaaaaggt
    17041 tcatcatctg gcgtgtaggt aatatcgttt gatcataata agaccgtaat ccttctaagt
    17101 cgccttctga aatatagctg tgaagagaaa aaagcagatt ttggtaatca tgtttgaatt
    17161 ctctcaagtc ttgatagttt ttttctagat ctttgatata atcctgttgc atcctgactt
    17221 gttgctctaa taactgatca gctaatttct tattagagat tttccaataa taaaccaaga
    17281 tgatcaggcc aacacacaga tatagaatga aaaaactcgt attcaaagct aagacatcag
    17341 gtgtttcacc agaaaaacgg gtataaaaaa taatgatata gtatgtaaat aatatcaatg
    17401 tcccgatcgt accgatcata cggcggtcca cagtgtgaaa ccaagtctct aacgattttt
    17461 ttaaacaaaa agctaataaa gcagataaaa aagcggataa gacaatgtga aaatactgca
    17521 atgaatctcc tggttcaata accgaagatt tcaaaacaaa aaaatcgccg atgcttgtta
    17581 agtgatctcc taaaagcgac gtcaataacc caaaaggaag cgcgtaaacc aattgttgac
    17641 gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc tatagcaatt agagcagtaa
    17701 aaatccctaa aatcgaatag acccatccaa tcaaaataca agaaatcaga atgactcccg
    17761 caaacagata tttgtatcgg atgtgatggt caatcaataa gatacaaatt gtgtaaatca
    17821 aatattgcaa cgctatggac cataaaaaca taaagtacag ctcccattga tttttaccaa
    17881 attatagcac aattggcgta ttattttttt aatttttcag ggattttgaa ttcagaactc
    17941 ataccaaagc agcagccaga tacaaaaaag tcagataagt tagttaattg tttaatgatt
    18001 gtttttttca tgaaagattc ctccaaaatt catcaagtag tttgtaatag gaagcaacgt
    18061 agctatttct aaaacaatcc caagtagcaa ataggtagaa aagtaagaag ctgagaaaaa
    18121 taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc ttctttaata atgggggacg
    18181 tttctcgggt tcaatgggat tgattgctgt acctcttgat ccatagcgaa gcagtaacaa
    18241 aaagcagctg attaacaaaa agaccatcat tgggaaggag atagtcaatt gcagatgttt
    18301 catcaccaag ggaacgcccc ataaataaag tagtgtccac agcagacaaa cgaattcaga
    18361 gtcggaatgc aatccataag catatctgcg gatgcagata taagaaagaa aggtgattcc
    18421 tgtttccgcc catgagcctg tcaccagaga aaagactaga acgaccgcta gttttccaag
    18481 attattcaat aaaacttcta agacaaattt tacttggaca tacaccatat cttcttccga
    18541 tttgtctccg ctgaataagg cttctaataa tttgttcaca actttatctt tcatctcata
    18601 cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa acgtcttttg tttcataaaa
    18661 agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa atcttcgttt tccaagaacc
    18721 ttaccaaaaa aagtgacagt tttgatacga aaagtacttt ttatgacaag tttgtgcttt
    18781 ttcgagtgat ttatgttaaa aaattactga ccttataaaa ggtgatggag ggattcttat
    18841 ggaaaatgaa caattgtttg aagtcggcga aacatatgca tttaaatctg atttatttga
    18901 atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat
    18961 gaaatgcgca gtagaagaca atgataccgc tcataatctg cactggcagt tagttgtacg
    19021 caaatcaagt acaatagaag aataaaaaca gataaaatgt tgagagcata aagaggttgt
    19081 gagtctcccg tttagctctg agttacaagg aataatgtta aaaaacagct tttcatgttt
    19141 tatgaatatt gccttgtgac cgaggtgttg gcaggagaat actgtttatt tgaaaaaagg
    19201 gctgcgacaa gacttttgtc acagccttta ttttcatgaa taggagttac ttttggctac
    19261 ttttagagaa gtagctttta aggagcagat aaagtcaagg cttttatttc tataaatgaa
    19321 gtagtaaaat gaagaacaga ttttattaca ggaaggatgc cgataattgt gatcattgtt
    19381 gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa tgcttgccca agatttagaa
    19441 acaaaacctt tttatgagaa cgtggaagat aatgaagtat tgccgctatt ttattctaat
    19501 ccagaaaagt atactttttt attacagatt ttctttttga acaaacgctt tttggcaatc
    19561 aaagatgctt tcagccatga tgacaacgta ctagaccgct ccatctacga agatagtatg
    19621 ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag tagaagtaaa gcagtatgaa
    19681 ggtcttttag aaacgatgct gaaggagtta gaagaaattt cgccacaaaa gaaaccagat
    19741 ctactggttt atattcgtgt atcctttgaa actatgttag cgagaatcaa aaaaagaggc
    19801 cgggaatatg aacagctaga acaagatcct gaactctatt cctactataa agaactcaat
    19861 cgacgatacg aagagtggta tgaacagttt gatatatgtc ccaaaatagt gatcgatggg
    19921 gacaaatatg atttcgtagc cgatccggca tgtggagaac aaatcgtaca agaaatcaag
    19981 atgcgggcaa agaaaatgac agaagaggca gatgcccagt cgatccgcta cactacaaat
    20041 aacccgaaag caaagggaat ctttacaact aaataatata aaaactaagc aaattgctca
    20101 ctcgcagata tttctctgtg agcagcatct tgcttagttt ttttgattca ttgagatcaa
    20161 atagtgaacc gattcgtatg ataaaattgc cataaatgaa atgtctgagt gagggccatg
    20221 atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc caaacagatg atgacagaaa
    20281 acaaggacaa tagctcctgc gataaaggca agaacgacac ttagatagtg gaaaaatcga
    20341 tcaaaatctg ctttttcttt agaaatacaa taatcatata agttcgttgc tacccctttt
    20401 aaatttccag ttgtaaataa attagtgtag gcacgcccgt tgattttatc aaaagctacc
    20461 cactggatcg atgcagtaaa agcaatcaaa atcgttacta atgcgggatg ctcatggacg
    20521 ggtgtcaaac taacaaagaa aaaaatcact gcttcataat atagaacaaa caagcgccag
    20581 aaatgttttt ctttttttct aaaataatca attagatact ttgttagaag aatccctaag
    20641 aaaaagaaaa gggtagacaa tactttttta ccaacactta gccattctcc gtcaaatgct
    20701 tggatgatcg atagaatgat attgccagtt tgagcagagg caaaagcacc atattggata
    20761 taagtataac tgtccatggc tcctccgata aatgtcagca aaagtcctat tgtacgcccc
    20821 tcgtgaaagg aagtctgcat ttttttcatc accttttatg aatatttttg tcttccttat
    20881 cttagcataa acgattgaaa aaaaggaaaa attttcagaa aactaaaatc atatcgcttc
    20941 ccattttcat ttttagaaag gtctgttagt ataaagaaaa aatagacaac gaaactttaa
    21001 ataaccaact tgaaataaac aaattcaaag aagaaagatg ggaaggaaat caatcagttg
    21061 aatgaaaaag aaattctccg attacaaaaa gaattgaaac gctgggagaa caagtataaa
    21121 aagcttcaac aagtctttcg gctacaagaa gaaatcattg ctacctatga agcggcttat
    21181 ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa aagaaagtga aggaaacagc
    21241 tctttcttta aaatgatgga gaaacgttcc tataatcgct tcaatcaaga acaaaaagca
    21301 gctatcacat atgatatgaa gaaacatctt cgaatcatag caggagctgg gagcggcaaa
    21361 acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc aaaaacaagt agacgaagaa
    21421 cgcatactaa tgatcacttt tacaaggaac gctgcaaatg aactaaaaaa acgagtagat
    21481 aatttcagcc agagaaaaac cgctgtccat atcggaacat tccatagtat cttttttagg
    21541 atatacaatg aaatttgtcg aaaatttcct gaagtagcaa tgcaaggaat acaaggagac
    21601 ttttcaaagg atactgcaca aaaagtgaat gctgttttgc aacagctgat acgtaaatat
    21661 aatctttata tatttgatca atatggagaa aaaacgattg cttctcggtt agactattgg
    21721 cagaatatga acctttcagt agaagaaatg gttcagttag ttaaagaaaa atatgattcc
    21781 attgataaga atgcacgaca gccgatatca gaacgactgt atggattgct cacggaactc
    21841 caagaacaaa aaagaagcca gcaattgcta gagttcaatg atgtactcca aaatctgaaa
    21901 agcgctttgg agaatgagga gatccagcat tatatcggcc aaaaatatga ttatctattc
    21961 attgatgaat ttcaagatac taatccactt caatgggaaa tcgtgaaacg gatgacaaag
    22021 gaaaacagga tcaagttgat tgttgttgga gatgatgatc agagtatcta tggattcaga
    22081 ggatcagagc cagcatacat caagaacttt gaaaaagaat atcctaccaa aactcttttc
    22141 ttattaacta attatcgttc tcgggcagct attgtccaag gagcaaatag attgatcagc
    22201 tataataaaa atgatcggat accaaaggcg atgattccag cacaaaaaga agcaggtgtt
    22261 atggaagcct atttgttttc tgatactaaa gcagaaagtc agtggctaat caaacaaatt
    22321 cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa tcatattata ccgatcagct
    22381 tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga atattccttt tgtactggaa
    22441 gcagatagtc catatgaagg tatctttggt atcaagtctt tccaacattt ctatcaaaaa
    22501 attgttcatt ggcaaacagc actgaatctg caaaagaaaa atcaggccta ccagcaattg
    22561 ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg aatgcgatca atacttttct
    22621 cctaaatatc aaaatatagc cgttgcagat tatattttgt cgataagccc caatttaaaa
    22681 tcgaaatctg cagaaatccg agagtttgag gaagcgttgc gtaaaacaga ccagcatcaa
    22741 gaaatctatt cgcttgttca aagctatctt catctgccac gtatagctaa ggaaatcgac
    22801 cagagtgatc aagaatggat acgagaagaa gtaaagcagc atgctacttt cagaagactc
    22861 caagccttat cgcaagaaac ccaagcgaca gctaaagaat taaaaacccg cttgattgct
    22921 tacagacaag gaaaacttga tgcactatgt atccagagca ttcacaaaag taaaggtctt
    22981 agttaccgaa atgtcttttt gattggttgt aacgaaggaa gtttgcctta taacggagcg
    23041 acagaaaaga aagtaatcga ctttaatgaa ataaaagcag aaccagtaac gacgatagaa
    23101 gaagaacgaa gactatttta tgttgcgatg actagagcaa gaaatcgtct gtatctatgt
    23161 gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga gatttatcaa agaaaccggg
    23221 agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg acagaagtgg tcagtttcaa
    23281 gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt tgtgaaattt cagcttattt
    23341 tcgaagaagc tatttctgga acacagttta ttcggaaaag ggaggtgtga caagaatttt
    23401 gtcacacctc ccttatttat tttgaaaatg agaatactat tatcaaaata gagaatatat
    23461 agaaaaattc tcattattca aacgtaaata atcagttatt ttgatttttt ttatatttta
    23521 ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata attaaaaagg
    23581 agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga
    23641 gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta
    23701 acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt
    23761 tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag
    23821 caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat
    23881 gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa
    23941 attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag
    24001 ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa
    24061 ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg
    24121 cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca
    24181 gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg
    24241 aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat
    24301 tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt
    24361 aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag
    24421 tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata
    24481 gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa
    24541 tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc
    24601 aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga
    24661 taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac
    24721 ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctg
//
LOCUS       DACJGB010000043        24541 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.043, whole genome shotgun sequence.
ACCESSION   DACJGB010000043 DACJGB010000000
VERSION     DACJGB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.043"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(27..602)
                     /locus_tag="IYI34_001755"
     CDS             complement(27..602)
                     /locus_tag="IYI34_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR0559624.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(607..1581)
                     /locus_tag="IYI34_001756"
     CDS             complement(607..1581)
                     /locus_tag="IYI34_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HAR0559625.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1605..1649)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1864..2832
                     /locus_tag="IYI34_001757"
     CDS             1864..2832
                     /locus_tag="IYI34_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR0559626.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2929..3222
                     /locus_tag="IYI34_001758"
     CDS             2929..3222
                     /locus_tag="IYI34_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAR0559627.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3340..3576
                     /gene="secG"
                     /locus_tag="IYI34_001759"
     CDS             3340..3576
                     /gene="secG"
                     /locus_tag="IYI34_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAR0559628.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3688..4434
                     /locus_tag="IYI34_001760"
     CDS             3688..4434
                     /locus_tag="IYI34_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR0559629.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4437..6794
                     /gene="rnr"
                     /locus_tag="IYI34_001761"
     CDS             4437..6794
                     /gene="rnr"
                     /locus_tag="IYI34_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAR0559630.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6816..7280
                     /gene="smpB"
                     /locus_tag="IYI34_001762"
     CDS             6816..7280
                     /gene="smpB"
                     /locus_tag="IYI34_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAR0559631.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7582..8301
                     /gene="atpB"
                     /locus_tag="IYI34_001763"
     CDS             7582..8301
                     /gene="atpB"
                     /locus_tag="IYI34_001763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAR0559632.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8354..8569
                     /gene="atpE"
                     /locus_tag="IYI34_001764"
     CDS             8354..8569
                     /gene="atpE"
                     /locus_tag="IYI34_001764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAR0559633.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8660..9184
                     /gene="atpF"
                     /locus_tag="IYI34_001765"
     CDS             8660..9184
                     /gene="atpF"
                     /locus_tag="IYI34_001765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAR0559634.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9171..9713
                     /locus_tag="IYI34_001766"
     CDS             9171..9713
                     /locus_tag="IYI34_001766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAR0559635.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9736..11292
                     /locus_tag="IYI34_001767"
     CDS             9736..11292
                     /locus_tag="IYI34_001767"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAR0559636.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMGVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11306..12208
                     /locus_tag="IYI34_001768"
     CDS             11306..12208
                     /locus_tag="IYI34_001768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAR0559637.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12231..13637
                     /gene="atpD"
                     /locus_tag="IYI34_001769"
     CDS             12231..13637
                     /gene="atpD"
                     /locus_tag="IYI34_001769"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAR0559638.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13651..14073
                     /locus_tag="IYI34_001770"
     CDS             13651..14073
                     /locus_tag="IYI34_001770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAR0559639.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14542..14772
                     /locus_tag="IYI34_001771"
     CDS             14542..14772
                     /locus_tag="IYI34_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAR0559640.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14869..16170
                     /gene="murA"
                     /locus_tag="IYI34_001772"
     CDS             14869..16170
                     /gene="murA"
                     /locus_tag="IYI34_001772"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAR0559641.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16183..16359
                     /locus_tag="IYI34_001773"
     CDS             16183..16359
                     /locus_tag="IYI34_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAR0559642.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16494..16790
                     /gene="yidD"
                     /locus_tag="IYI34_001774"
     CDS             16494..16790
                     /gene="yidD"
                     /locus_tag="IYI34_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAR0559643.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16854..17321
                     /locus_tag="IYI34_001775"
     CDS             16854..17321
                     /locus_tag="IYI34_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0559644.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17406..18290
                     /locus_tag="IYI34_001776"
     CDS             17406..18290
                     /locus_tag="IYI34_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR0559645.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18407..18640
                     /locus_tag="IYI34_001777"
     CDS             18407..18640
                     /locus_tag="IYI34_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAR0559646.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18728..18808
                     /locus_tag="IYI34_001778"
     tRNA            18728..18808
                     /locus_tag="IYI34_001778"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18762..18764,aa:Tyr,seq:gta)
     gene            18815..18886
                     /locus_tag="IYI34_001779"
     tRNA            18815..18886
                     /locus_tag="IYI34_001779"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18847..18849,aa:Gln,seq:ttg)
     gene            19402..20109
                     /locus_tag="IYI34_001780"
     CDS             19402..20109
                     /locus_tag="IYI34_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0559647.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20168..20812)
                     /locus_tag="IYI34_001781"
     CDS             complement(20168..20812)
                     /locus_tag="IYI34_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAR0559648.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20939..21226
                     /locus_tag="IYI34_001782"
     CDS             20939..21226
                     /locus_tag="IYI34_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559649.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21334..21654
                     /locus_tag="IYI34_001783"
     CDS             21334..21654
                     /locus_tag="IYI34_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0559650.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21972..23048
                     /locus_tag="IYI34_001784"
     CDS             21972..23048
                     /locus_tag="IYI34_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAR0559651.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23045..23764
                     /locus_tag="IYI34_001785"
     CDS             23045..23764
                     /locus_tag="IYI34_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAR0559652.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23892..24443
                     /gene="raiA"
                     /locus_tag="IYI34_001786"
     CDS             23892..24443
                     /gene="raiA"
                     /locus_tag="IYI34_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAR0559653.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         8130 a         4281 c         5275 g         6855 t
ORIGIN      
        1 tattttgcta atccagctct gtttattcag cctatttttc tttttgaata tatcaagcgt
       61 tgtggcaaca tagtgatgac taatgaaaca acgacgatag acaacaaacg atcaaggtag
      121 tctgtacctg cctgtaccaa gatcacactt gttgtctgac ttaaacctaa cccatgtaat
      181 agctgaacga tcatactgga tcctgaggag gtgatcccgc caaataactg aacggtaatg
      241 atcgaagaaa cgatcgttcc aggtaccgac aaaatcaaag cgagtaataa cagtttcaag
      301 ctgtttttat ctttgccgag atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga
      361 atttggattg gcgaataata cagagaaaaa atatctgtag tcactccact taaaataccg
      421 ctggacaatc ctgtcagcat tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt
      481 ccgatagaat cgagatagat tggcaaacga agccataaag caatcgttcc tccaatataa
      541 ttgatcccaa tacacaaagc catcactgta agtacgcgag ttgccatttt gtttttattc
      601 attttgttag cccatcttta acctttccaa atctttcata atcgtttctt cttgtgcatt
      661 taaaagaacg gttaaaaact ttcggaaaaa aagacgagta tctacttttg tcataatttt
      721 actgttagca ggtttctgcc aaaattcata acgatcaacc aatgtttgtc cacgacttat
      781 tccttgagtt tccacagcag tgaagctttt gaaaccttca cagatggcct cctcgataaa
      841 ataagcaaca gctaacggat cattgatgac acatcctaaa attctttctt gtttccagtg
      901 gaagtcaaaa taaaaacgag tgatcgcttt tagatactct ccctcttcag gattcatttg
      961 acagcagtat tcgaggatag tcggtgtcaa gacgatttct cttgtcacat ctaacccaac
     1021 catttctatc gtttgtttga gattctcgaa tacataagaa gctgcttcag gatcacacca
     1081 gtaattgtat tcagcaactg gcgaacagtt accatggctt ttgtaagttc cacccatcga
     1141 gacaaaacgt tccatatgtt tcccaatatt tgggttgact ttcaaagcag aagcgatatt
     1201 ggtcaaagga cccaaagcaa tgactgatgt atttgtcttt tctttgaaat aatcagccaa
     1261 aaaatcaacg gcatggagag gttctgcctg ttttctcaag atcatcggga aatttgtttc
     1321 ccctaaacca tccataccat gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt
     1381 ttccattcct tgataaacag gaatgtctaa acgatcacag cgttccaggc atttcagcac
     1441 attctgcgta cctatatctg cagggacatt cccacaaact acggtaatag caactacttc
     1501 gatatcaggg gatttgacag ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca
     1561 atcaataatt acttttcgca ttatttccaa tctcctttct ttagagatac ttagtttttt
     1621 attctgggaa gtttccgaac cagtccaaga cgagcgtctt gattataatg cacgaaatta
     1681 gtatactggc ttttattgcg aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa
     1741 ctttaagtaa atgatacaat gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat
     1801 cctatggatc ggtacattgg ataatagata gattatagtt tggcagaaag cggcgggtgt
     1861 acaatggtta ttttttattt cattcttatt ctgataatta tagggatcag ttaccatttt
     1921 ctttcttatt tttatgcaaa gaaattagta agagcgggag tacagagagg atatgactgg
     1981 tatgaagaca ctggtcatcg tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa
     2041 cgacaaaaat tagaagaaga gtgtgaaagc ttttgggagc agggaacacc caaaacaatc
     2101 aaaagttatg acgggttgaa actagctggt cagatgttca tccatccaaa taagcagaat
     2161 aaatgggtca tttgcgtaca tgattaccga agcacaggga aaagagacat gtctcatata
     2221 ggtaaaagat atgcagagaa aggattcaac gttcttgtac ctgatttacg tgcgcacgga
     2281 gaaagtgaag gagaaatcat cggaatggga tggctagatc ggctagattt aattgcctgg
     2341 atccaactca tcttagatga acagccgaat gcgtccatta ttttacacgg cggatcaatg
     2401 ggagcttcta cgatcatgat ggcaagtggt gaaaaacttc catcagcggt caaaggattt
     2461 attttagaca gcggatatgt ttctgtttat gcagaattcc gatatatgtt aagtaaaatc
     2521 actgtctttc ctaaaaaaat ggtcatgaga tatgcgaatc attatgccca gaaatacgcc
     2581 ggttactcct taaagcaagc atcagctacg agacagcttg gcagcaatca tctgccttta
     2641 ttagtcatcc atggtgaaag agatcatttt gtccctacag aagcagcgta cacgattcaa
     2701 aatgctacgg caggggataa agcactgctg cttgttccgg aagcagaaca cttggaagca
     2761 tcgatgaaag atccgaaaac ctattggaca gtgatttttt catttattga acaaagaata
     2821 acgatgaatt aattttctct tcttttttgt aagtaattcg aggataaata attgtttata
     2881 gatcaggaga ctgctaaaat aaggtaggaa taaaaaaata tcagttattt gaggagtgcg
     2941 gcatatggaa atcaaagaag aaaaaatcgt cttgctttat tcaatgatga acaacaagag
     3001 atcggagaaa tgacatggtc agatgcgggc ccagacatca tgatcgttga ccatacattt
     3061 gtagatccag catatcgtgg tcaaaagtta gctgaaaagc tagtgtatac tggagtagaa
     3121 ttagcaagac gagacgggaa aaagatcatt cctttatgcc cttatgcaaa aaaagagttt
     3181 gaaaagaaac cagaatatca agacgtctta cgtcagtcat aagaataaca gctgaaagtt
     3241 ttgaactttc agctgttatt ccatttatat agagttgtca cggactttta tctatgataa
     3301 agttaaagag acgatatctt ttgggaagga ggctgcgata tgtataattt aattttggga
     3361 atcgtgatcg ttttatcgat tgttatggtc attgcgatta tgatgcagcc aagcaagcaa
     3421 aatagtgcag cgagtgcatt taccggcggt gcggatcaac tattcggcaa acaaaaagct
     3481 cgcggctttg aagctgtcat gcagcgatca actgcagtta tgggagctat ctggatgatc
     3541 ttattatttg tcctagcata tttatcttcg aagtagttga ataagcctct cgcaaaacgg
     3601 cgggcggctt tttttattgc aaagagaacg cttaagaaga agttatgaaa gaaactatgg
     3661 taaaatgagg cgaaagaggt gagacgagtg agacaattac caaaacccct ttatgcaaaa
     3721 catggaaagc gagcggtttt gctgcttcac gcttactcag gaagtccaaa cgatgttcga
     3781 atgctcgcac gatttttgga aaaagcagat tatacagttt atgcgccgct ttttaaagga
     3841 cacggaacaa tggagccata tgatatcttg caagaaaaag cagaaagctg gtgggcagac
     3901 actaaaaaag ccatccattt tttacagtct gagcagtttt ctgatattgc cgtattagga
     3961 ttatcaatgg gcggcatctt tgctgtacga gcactagaag aagaatcagt gattggcggc
     4021 ggttttttct gttcgccttt gtctcctgta aaaacaaacg tgcctgaaaa ctttgaaaaa
     4081 tacgtacgtc aagtgctgaa gacagcaggt aaatcagaga aagaaatcaa tgaaaaagca
     4141 gttgcttacc gctcgttagc tgaacaacaa ttaatggata tacaagatca ggcagcaatc
     4201 gtagaaagta ggttgtcaga tatccaacag ccgatatttt tagctcaagc tgggaaagat
     4261 gaaatgatcg accctaatgg cgtatttgaa actgcacgca agttgagccg tcagcgagtg
     4321 acacttcaat ggtatcctga aagtggtcat gtgattacag ttggtacagc aagacgcgaa
     4381 ttagaaaaag acgtcttgga atttttagaa aaattacctt ggaatgagga ataaatatga
     4441 caaaagaaac gataaaagag aaaattctct ttttcatgga aagcagtcgc aaacgcagtt
     4501 tttctatgga agaaatcgct gaaggtttag ggttccaaaa aagcgatgat tttaaattac
     4561 tagtacaaac gattgctcaa atggaaagag aacagagtat agttttcaat aaaaaaggaa
     4621 aagtgaaact tcctttaaaa ccaatcctga tcgaaggaac atttcgagca aatgaacgag
     4681 gatttggatt tgtaacgatt gatcccgaag aagatgacat ctatatccca aaagaagcga
     4741 ctggttttgc aatggatgga gacaccgtag caatcgatat catgaagcga gcaaatacag
     4801 atatggatcg cggagcagag gggaaagtag tcgagattcg agaccgggcg actactcagt
     4861 tagcagggga attcgttgct tattcagaag aagaaataca agaaacagat ctttacggcg
     4921 ttgttatccc gaaagataga aagctcaacc aatttaaagt atatgcagca gcagagggag
     4981 ttcgtccagt tgacggaagt attgtgctga tcgaactgac ccattaccct gaaaaagggt
     5041 atgcgactag tttagaaggg ataatcaagc aagtgattgg tcataagaat gaccctggca
     5101 tggatatcct ctcaattgtc gtggctaatg gtatccctac gaaatttcct gatgatgtat
     5161 tgtctgaagc agatgaagtt ccagatcaaa tcagcgaaaa ggatataact ggacgtagag
     5221 atttaagaaa tcagctcatt gttacgattg atggagaaga tgcaaaagat ctagatgatg
     5281 cagttacggt taaaaaatta gataacggta attattttct tggagtacac attgcagatg
     5341 tttcttacta tgtgacacaa ggcagtcaat tggacagaga agcatatgaa agagggacaa
     5401 gtgtatattt gactgatcga gtaattccga tgatcccgca acgtttatcg aatggtatct
     5461 gttcactgaa tccccatgtt ccaaggctga ccatgagttg cgaaatggag attgatccaa
     5521 atggacaagt agtctctcat gatattttcc caagtgtgat ccaaacaaca gaacggatga
     5581 cctacacagc tgtcaatcaa atcttagaag atcaagacga agaaacgatg caacgctatg
     5641 ctcagctcgt acctatgttc caagaaatgg gtgaattgca ccgaatcctt gaaacgatgc
     5701 gaatcaaacg tggcgcgatt tcttttgagg atcgcgaagc gaaaatcctt gttgatgctg
     5761 aaggacaccc aaatgacatc cagctaagaa gcagaggaat tggtgaacgc ttgatcgaat
     5821 ctttcatgct tgcggccaat gaaacggtag ctgaacatta taataaatta aatctgccgt
     5881 ttatttaccg aatccatgaa cagccaaaag aagaaaaaat gcagcgcttc tttgattttg
     5941 cttcagcttt gggcattctg gtccgaggaa cgaaaggaac gatcacacca aaagatttac
     6001 agcatgtgat cgaaggagta gaagacaaac cagaagctgc agttatcaat actatgttgc
     6061 taagaagcat gcaacaagca cgctattcag aagacaattt tgggcattat ggcttagcgg
     6121 cagattacta cacacacttt acatctccta ttcgacgcta tcccgatcta atcgtccatc
     6181 gtctgatccg ttcttacagt gaagataaaa gtgaaagcaa tcaaagttcg tgggctgcgg
     6241 aacttcctga aattgcagat catagttcta agatggaacg tcgtgcagta gaaacagaac
     6301 gtgaagtaga ttccatgaag aaagcagaat tcatgatgga caaagtggga gaagaattcg
     6361 atgggatcat cagctctgtt gtgaaattcg gcctgtttat cgaactgccg aatacagtag
     6421 agggattgat ccatatcaat gaattgaaac aggactactt ccattttgtt gaaaatcatt
     6481 tagctcttgt aggtgaaaga actggtctga cttttaaaat cggacagaaa gtgcgagtaa
     6541 aagtagtcaa agctgatcca gatgaacgag cagtagattt tgaacttgtt tccgcagaag
     6601 aggttacagc gttggaacgg ccaaaaacac gaaatggaaa atcaggaaaa cgcgaaggaa
     6661 aacaagcagg aaaacgccca gaaagaaatg gcaagaagaa caaacgatat agcgatagta
     6721 agcaaacgtt cggtaagaaa aaagggaaga agccatttta caaaggtgtt aaaaagaaga
     6781 aaaagaaaaa ataaaatgag ttggaggaaa cagctatgcc aaaaggcgag ggaaaattaa
     6841 ttgcacaaaa caagaaagct cgccatgatt attcgatcat cgacacgatg gaagcaggga
     6901 tggtcttgca aggaaccgag atcaagtcga tacgaaacag ccggatcaat ctaaaagatg
     6961 gatttattcg cgtccgcaac ggagaagctt tcttgcataa tgttcatatc agtccttatg
     7021 aacaaggaaa tatttttaat catgatccgt tgcgcacgag aaagttatta ttacacaaaa
     7081 aacaaatcat ccggcttgaa aatgaattga aaaatactgg aatcactgtt gttcctttaa
     7141 aagtctatat tcgtaacggc tatgccaagg tattgattgg tctggcgaaa gggaaaaaat
     7201 cttatgataa acgggaagat ttgaaacgaa aagatatcga tcgacaaatt gatcgaacat
     7261 taaaaaattt ctctagataa tttcattatc ctttttcgca tattcggtgc ttttgctttt
     7321 acttaatgat aaaatttgtt agcatttaat ctgataaaac atgattatca taggtttttt
     7381 cttagatcag gagcaccgtg tataaagaaa aattcgcttc gtttttgact gaaataaaaa
     7441 agtgtctaat tttcatgaaa atgttttgaa ttttctgttt catggtggta tgataccgtg
     7501 tggttagaaa ccgattggaa aaaaccagat acgcacatga attgatttta tgtgaatttc
     7561 ggggaaaaga ggtgaaatgg attggatgaa cgctcgctaa cgttccacat tggaccaatt
     7621 tggtttgatg gtacagtttg tataatggtg ttactgacct gtttgattgt tttcttctta
     7681 gcttactttt ttacaagaaa tcttaagttg aaaccaacag gcaaacaaaa tgcgatagaa
     7741 tgggtcattg attttagtcg aggaatcgta acagataatc tgcctagaaa agaattgagc
     7801 aatttccatt tgttggcatt tacattgttt ttatttgttt ttgttgcaaa caacatcggt
     7861 ttagtaacaa aaatcgtttt acctaacgaa acaacacttt ggaaaagccc gacagcagac
     7921 ccgtttgtta ctttaacttt ggcatttata atgatcttac ttactcactt attcggggtg
     7981 aaaaagttag gcttcaaagg atatttcgta aattcgttct tgaaacccta tagttttatg
     8041 tttccgatga aactgattga agaatttaca aacttattaa cacttgcttt acgtctttat
     8101 gggaatattt atgcggggga agttttgcta accttgattg caaaaatgat gacaagctta
     8161 ggctggttct cattaccatt agcaattcca ttagagatgg tctggatcgc gttctccttg
     8221 tttattggga gcatccaagc atttgtcttt gtcactttgt caatggttta tatgagccat
     8281 aagattgaag tagaagaata atactcggtt aaaaaacaaa ctactattta tctattttag
     8341 gaggaaaaca attatgaatt atatcgcagc agcaatcgca atcatgggag cagcaatcgg
     8401 agccggttat ggtaacggtc aagttatctc taaaacaatc gaatcaatgg ctcgccaacc
     8461 tgaaatgtct ggtcaattcc gtacaacaat gtttatcggg gtagctctag ttgaagctgt
     8521 tcctatctta ggggtagtta tcgccttgat tctagttttt gccgtttaat tgaatcagta
     8581 ttaaggcaga aacaacatcg tttgttactg cttttttaca tggaaactag aaatactcca
     8641 aagaaaggaa ggctcgccta tgctgaatca attggcaatt gcagaagttg gcaatgcgat
     8701 gttaggtaat atcatcgttg tcagcggctc atttttgata ctactggctc tcttgaagca
     8761 ctttgcttgg ggaccaatca gcgatatttt gaaaaaacgt gaagacaaga tcgccaatga
     8821 tttagattct gcagaacaat ctcgcatcaa ctcagcgaaa atggaacaag aacgcgaaca
     8881 acaattgtta gcctctcgtt ctgatgcagc tgatatcatc aaaaatgcga aagaaagtgg
     8941 agaattaagc cgccaaaata ttttgaagga tgctcaagaa gaagcagctc gtctaaaaag
     9001 caaagcccaa gctgatatca ctgtagaacg tgattcagcg ctgaactctg taaaagacga
     9061 cgttgcagaa ctctctcttc aaatcgcgga aaaaatcttg aacaaagaat tatctccaga
     9121 aatgcatgaa tcattaatca atcaatacat tgaaggtcta ggttcttcaa atgaaactag
     9181 ataaatatac agtaggaaga cgttacggga aagctttatt tgaactggcg atcgattcaa
     9241 atcaagcaga agaaatctat caagatctcg taagtttacg tcaaatctat gaacaagtac
     9301 ctggattagg gaatatgtta agtgatgttc gtctagaacc tcatgaaaaa cgagaaatca
     9361 tggacaagct tgtcagcggt tatgatggga tcgttaaaaa ctttttagaa gttgtttatg
     9421 agtataaccg aatggatgac cttttgttca tgatcgaaga atatgaacgt cgatatgatg
     9481 aacatcaagg gttactattg ggaagtgtca caacagcagt cccactttca gacgaacaac
     9541 gaatacaatt agaaaagaat gttgcaaaaa caatggacta tcaatcagtt gaattaaaac
     9601 aaatcgttga ttcttctatt attggtggcg cgattgttga agccaaccat cgagtaatcg
     9661 acggaagcat tcgcacgcaa ttagaaaaaa tgcgtaatca attaaacaga tagtacaaag
     9721 aggtaggtga atcgaatggc catcaaagca gaagaaatta gtgccttgat taaagaacaa
     9781 attgaaaatt atcaaaacgt gctttcagtc gaagaaatcg gtaccgtaac ttatgtaggt
     9841 gacgggatcg ctcgtgcaca tggtttagaa aacgcgatga gtggtgagtt gcttgaattt
     9901 tcaaacggct catacggaat ggcgcaaaac ttagagtcaa atgatgtagg tatcattatc
     9961 cttggcgatt tcgagtccat tcgtgaagga gataaagtaa aacgaacagg taagatcatg
    10021 ggagttccag ttggagaggc cttgatcggt cgggtagtca atccgctagg tcaaccaatc
    10081 gacggactag gtgaaatcgt tactgacaaa gctcgtccag tagaagcgat ggcaccaggt
    10141 gttatgcaac gtaaatctgt taacgaacca atgcaaacag ggctaaaagc gatcgacgcc
    10201 cttgtgccaa tcggacgcgg acaacgtgaa ttagtcatcg gtgaccgtaa aacagggaaa
    10261 acttctattg cgatcgatac gatcatcaac caaaaaggtc aagatatgat ctgtatctat
    10321 gtagcaatcg gacaaaaaga ttctacagtt cgtacacaag ttgaaacatt gaaaaaatat
    10381 ggcgcaatgg attacacaat cgttgtgaat gccggtgcgt ctcaaccagc accattgctt
    10441 tatatcgcac catatgctgg tactgcaatg ggtgaagaat tcatgtacaa cggtaaacat
    10501 gtattgatca tttttgatga tctttcaaaa caagctgttg cttatcgtga actttccttg
    10561 ctacttcgcc gtccaccagg tcgtgaagct tatcctgggg atgttttcta cttgcactca
    10621 cgtttattag aacgtgcagc aaaattaagt gatgatttag gtggcggttc aatgactgcc
    10681 ttaccatttg ttgaaacaca agctggagat atctctgctt atatcccaac aaacgttatt
    10741 tcgatcactg atggacaaat cttcttagaa agcgacttgt tctacgcagg tactcgtcca
    10801 gcggttgatg ccggactatc tgtatctcgt gtaggaggtt ctgcgcaaat caaagcaatg
    10861 aaaaaagttg ctgggacttt acgcctagac ttagcaagtt accgtgaatt agaagcattt
    10921 acacaattcg gttctgactt ggatgcagca acacaagcaa aactaaatcg tggacgccgt
    10981 acagtagaaa tcttgaaaca aaaattgcat gcaccgctag ctgttgaaaa acaagtagtg
    11041 attttgtatg ctttgacaca tggattctta gatagtatcc ctgtagacag tattttagac
    11101 ttcgaacatg aattattcga atatctggat accaatcatt cagatatctt tgaaacaatc
    11161 cgtacaacaa aagatcttcc agaagaagaa gcgttgaaca gtgcgattca ggaatataaa
    11221 gacatgttct tagctacaaa aggaagtaat tcctctacag aagataaatt gaaatccatt
    11281 caaaatgcat agtgaggtga gatgaatggg tgcttcatta aatgaaatca agacacgtat
    11341 cgcttcaacg aaaaaaacga gccaaatcac gcgggcaatg cagatggtgt ctgcttctaa
    11401 gttgacaaaa tctgaagctt cttcacagaa gtttcaggtt tatgcaacaa aagtaagaga
    11461 aatcgttacg catttgactg ctactcagtt aaatgatatt gcctcagaaa atcctagagg
    11521 ggatatcaat tacaacagca tgctgctcag ccgtcctgtg aagaaaactg gttatatcgt
    11581 catcactgca gatggcggtt tagtcggtgg atataacagc tcgatattga aacaaacgat
    11641 gtctatttta gaagaagacc accaatcacc cgatgattac gtgatgatcg caattggcgg
    11701 aactggtgca gacttcttta aagcacgcgg tatcaatcta gcatatgagc tgcgcaatct
    11761 atctgatcag ccaagctttg atgaagtccg caaaattgta aatatggcaa caactatgtt
    11821 ccaaaacgaa gtattcgatg aattatacgt atgttataac catcatatca attcgctaac
    11881 tagtcagttt cgtgtagaaa aaatgctgcc aatctcagac ttagatccag aagaagcaac
    11941 gactttcgaa caagaatata tttttgaacc ttcaaaagaa gaaatcttgt ctcagttatt
    12001 gcctcagtat gctgaaagtt tgatctacgg tgcgatcgtt gacgccaaaa cagcagaaca
    12061 tgctgctggt atgacagcaa tgaaaacagc aactgataat gctgctaata tcattgatga
    12121 gctgacggtt tcttataacc gtgcaagaca aggggctatt acccaagaaa ttaccgaaat
    12181 tgttgccgga gcttcggcac tagaatagta ttgagattgg aggaaaaaag atgagttcag
    12241 gcaagattgt tcaagtaatc ggtcccgttg tcgacgtgga attttcttta gaccaatcct
    12301 taccagatat caacaacgca ttagttgttt ataaaaatga cgaaaataaa tcaaaagttg
    12361 ttcttgaagc agcgttagaa ttaggggacg gagtgatccg gaccattgcg atggaatcaa
    12421 cagatggttt acaaagagga atggaagtca ttgatacagg caaagcaatc tctgttcctg
    12481 taggtaaaga aacattaggt cgcgtattca acgtactagg agatacgatc gatttagaaa
    12541 cacctttccc agaagatgcg gaaagaagcg aaattcataa aaaagcacca gcctttgacg
    12601 aattaagtac aagtacagaa attttggaaa cagggatcaa agttatcgat ttgcttgccc
    12661 catatttaaa aggtgggaaa gtcggactat tcggtggtgc cggtgttggt aaaaccgtac
    12721 tgatccaaga actgatccat aatatcgccc aagaacatgg tggtatttct gtatttaccg
    12781 gtgtaggtga acgtactcgt gaaggtaatg acttgtatta tgaaatgaaa gattcaggag
    12841 ttatcgaaaa aacagccatg gtgttcggac aaatgaacga accaccaggt gcacgtatgc
    12901 gtgttgcttt gactggtttg acgattgcgg aatatttccg tgatgtagaa ggtcaagatg
    12961 tactgttgtt tatcgacaac attttccgtt tcactcaagc tggatctgaa gtatcagcct
    13021 tgttaggacg gatgccttct gcggttggtt atcaaccaac attggcaaca gaaatgggtc
    13081 aattgcaaga acgtatcaca tctacgaaaa aaggttcgat cacatcgatc caagcaatct
    13141 atgtgccagc cgatgactac actgacccgg cgccggcaac agcattcgcc catttggatg
    13201 cgacaaccaa cttggaacgt aaattaacag aacaagggat ttatccagcg gtagatccat
    13261 tggcttcttc ttcaagtgct ttagcacctg aaatcgtggg agaagaacat tataaagtag
    13321 caacagaagt ccaacacgta ttgcaacgat atcgtgaatt acaagatatc attgctatct
    13381 taggtatgga tgaattatcc gatcaagaaa aagtgttagt ttctcgtgcc cgcagagtcc
    13441 aattcttctt gtcacaaaac tttaacgtgg ctgaacaatt tactggtctt ccaggttcat
    13501 atgtccctgt agaagaaaca gtgaaaggat tccgcgaaat cttggaaggc aaatacgatg
    13561 atcttcctga agaagctttc cgaagtgttg gccgaatcga agacgtcgta gaaaaagcga
    13621 aaacactagg ctactagaaa ggggtgcccc atggatcaat atttaacagt aaatgtggta
    13681 actcctgccg gtttggtcta tgatcaccat gcagctatcg tcgttgcacg tacgacagct
    13741 ggtgagcttg ggattttgcc taaacatgca ccaatcatcg ttccattgac gattgatgaa
    13801 gttcgtgtaa aacgaacaga ttcagatacc cacgtcgatt ggatcgctgt taatggcgga
    13861 atcatggaag ttcgtgataa tgttgtttca atcgttgctg atagtgcgga acgtgaacgt
    13921 gatatcgatg tatctcgtgc ggaacgtgca aaacaacgcg cagaacgcca aatcgaagaa
    13981 gcaaaagcaa aacaagatac gaacgaattg aaacgggcaa ctgttgcgtt gcaccgagca
    14041 atcaatcgta tcaaagtatc taagcactca taaaatgaga tgcggatgtt tatccgtgtt
    14101 taagaacagt aaaaagaggt tatgacagaa gtggtcagct tcaagacata agaaggaatt
    14161 tacgaaaatt gcttctcaaa tttttgtgaa atctcagctt atttttcgaa gaagctactt
    14221 ctggaacacc gtatatcaga ggttgtgaaa tcagcgtttt gtcctgagta ttagagaaac
    14281 aaatggaaaa tagttcctca tattttgccg ttttttgagc taataccgag ggtcagatga
    14341 ttgaacaccg tttatttgga ataagggact gcgacaagac ttttgtctca gtcccttttt
    14401 ttgcatattt ttagcattta tttcctaaaa aatgtaaggg gattcctata tttttaagta
    14461 tacttgtttt tccttttata cattttatgg tattatcatt ttattagtta ttataaaaca
    14521 atcgttagaa aggacgtcat tatgcaagta tacggaattg atgcggtgct tcgaatcgtg
    14581 agtcatttgg cgtttatcta cttagccttc tggtctttac gttcgttacg cattgaaact
    14641 tttttcagac caatgcataa tacgcaaatt agaatggcaa tcgtactttt ttcaattttt
    14701 ttagggtaca ctgcaagtaa ttttttctta gaggttatcg ctttatgccg aaatcttttc
    14761 gttagcctgt aaattttaaa aaaaggtact attttaaaag caaggatgtt ataatagaaa
    14821 agattttgag taagtgtatg ctcattggaa ttttttggag ggaacaacat ggaagagatc
    14881 atcgtaagag gtggcaatca attaaatgga acagtaagaa ttgaaggagc taaaaatgct
    14941 gttttgccta ttttagctgc tagtttgtta gcagaagaag gtattacgac attagacaac
    15001 gttccgattc tttctgatgt cttcactatg aatcaagtga ttcgtcactt aaacgtagat
    15061 gtagattttg atgaacaaaa gaatcaagta acaatcgacg cttcaagaca attagaaatc
    15121 gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa tcgttgtgat gggtccgcta
    15181 cttgccagaa atggacatgc aaaagtagct atgcctggtg gatgtgcgat tggtaaacga
    15241 ccaatcgatt tgcatttaaa aggatttcaa gctttaggag caaaaattat tcaaaaaaac
    15301 ggctatatcg aagcaattgc ggatgaattg atcggtaaca cgatttattt agatttccct
    15361 agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa aagcaaaagg aacaaccatc
    15421 attgaaaacg tagctcgcga accagaaatc gttgatttag caaacatttt gaataaaatg
    15481 ggcgctcaag tctatggagc tggtactgaa acaatgcgta ttgaaggcgt ggatcattta
    15541 catgcagtca accactcaat cgtacaagat cgtatcgaag caggtacctt tatggttgct
    15601 gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta tctctgaaca taaccgccca
    15661 ttgatttcta aattgatcga aatgggtgca gaaatcattg aagaagaagg cggagtccgt
    15721 gtcatcggac caaaacatat cttacctaca gatgtaaaaa cgatgcctca tcctggtttc
    15781 cctacagata tgcaagctca aatgacagct atccaattag ttgcagaagg tacaagtgtg
    15841 gtgactgaaa cagtgtttga aaatcgtttc caacatttag aagagatgcg ccgtatgaac
    15901 gctcatgtca aaatcgacgg aaatgtagca atcatggatg gaaaccatga attgcaaggt
    15961 gcagaagtat atgcaacaga cttgcgggca gcagcagcat tagttttagc tggcttgaaa
    16021 gcaaatggaa tcacacgtgt gcgtaacttg aattatctag accgtggata ttataatttc
    16081 catatcaaac tacagcaatt aggtgcagac gtcgaacgtg tagacatgga tcaaacttct
    16141 gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct gaatgagtaa ccaacgatat
    16201 atcttattaa ctttgataaa gattttagtg gtgatcttat tgcttatcct tctttttgtg
    16261 gccggaacga tgatcggata tggtgtcatc ggtggcggca atccatttaa agtattccag
    16321 ccaagtttgt ggattcatat tcgagacttt tttcattgat aaataagaat cacagtggtt
    16381 ctagtcgata tgaagtgagc catgaagcag aacatgataa aagaaaacag aagggaacgt
    16441 tgacgttagt cgcgatccct ttttttagta aaagaaaaga aggaatctaa ttgatgaaga
    16501 agatattgat ttatttagtt agaggatatc agcgctttat ttcgcctgca tttccaccta
    16561 gctgccgtta ttacccaacc tgttccaatt atatgataaa agcaattcaa acccatggtg
    16621 cgctaaaagg gacggtaatg gggactgcac ggatcttacg ctgtcatcct tttgtcaaag
    16681 gcggtatcga ttatgtacca ttgacattcc gattaacgcg aaataagaaa gataaaaaag
    16741 aaccagatta taaaaaggtt gtgaaaaata cgggtagcac tgagcgataa agaaaaagag
    16801 gaaatttatt gtcaaagtaa caactccgca actacttata tgggggacaa aaaatgatcg
    16861 aagaactaaa caagaaagat ttaccatggt ccttgttatt agatgcagat cctgataaag
    16921 aaaaagttca agtatatgtt tccagaggga gtggctggat atggaaagaa aaagaaaaaa
    16981 caataggtgt actgatctat gtggcaaggg aaaaagaata cgaaatcgtc aatgtagcag
    17041 taaccccaac gtatcaagga aaggggattg gcggaaaatt acttgagaca gcttttcaga
    17101 agctttcaca gctagcttca aaccaaactc ggatcattat acgtactggc actactagta
    17161 gcgctgcgtt acacctctat caaaaaatgg gctatgtgga gattggtcga gtcaaagact
    17221 attttatcca tcattactct gagcccattt ttgaaaatgg agatcaatta agggatcaag
    17281 tgattttagc aattcactta cctaaaaata gctttaacta ataaactttc aattcgagaa
    17341 aatgctttca aatgctaaat agatacatta caatagcttt agcaaacaaa aaggagaata
    17401 tttttatggg aatattagtt tttgattttg gtggatcggc tgtcaaatat ggttgttggg
    17461 atggcaaaga gattaaaggt aagggacaat ttgcaacgcc tgaatcttgg gaagaaatga
    17521 aaacgcaatt atttcaagtt tataaaaaaa tactcgtttc ttttgaagtc gaaggaattg
    17581 gaatcagctc tcctggagta gtgaataatg agaaacaggt gattgaggga atcagtgcga
    17641 ttccttatat tcatggattt aatattttcc gagatttgga aactctgttt caactcccag
    17701 taacgattga aaatgacgcc aactgtgccg gaatggctga attttatgaa ggtgctgccc
    17761 gtgattatca agatgttgct tttgttgtgg caggaactgg gattggcggg gcgttgttca
    17821 cgaaaggaca aataaataaa ggggcacatc tttacggtgg agaatttggg ttgatgttct
    17881 tagatggcga taagacattc agtaaacttg gtacagcggt acagatggct tggcgttatt
    17941 gtgaacgcaa aggtctcgat aaaaatgctt ataccggaaa agatgtcttt gaattagcag
    18001 aaactggaga tgcaatagca aaagaagaag ttgagagttt ttatacgtat cttacaaagg
    18061 gattattcag tattcaattt tcgtttgatc cagaagtgat cgtattaggc ggcggggttt
    18121 ctgcaaaaga agggctcatt gatgagatca aaagccgaat gaagaagctg acagaacaat
    18181 tcgatctgca tgatttcgaa ccgcagattt tattatgtga gtacagaaac gatgctaatc
    18241 ttataggtgc agcagcaaac tatctcgctg tacgtcaagg agagaattaa gagctttaga
    18301 gaaggtgcag cttgcataaa agaagagaat gaaatgaata ggagattttt acctaaggaa
    18361 tgttttattt gctgcagtat gataaaatat ctgagaggtg aaaatgatgt caaaaaaaaa
    18421 taaagatatt gaagtgcgta tagaagaagt gaaaaaagat atcaaaggta agaattatga
    18481 agtcactcag ttatttatag ggaaaaaaat gattggtgag atcttagcct atgggccaaa
    18541 agaatatgaa attttctttg gtgaagaaga ttttggaaaa gaaaaaagtc ttgaaaatgc
    18601 gatagaaaca gtgattcgac actggaatct gcacgaataa aaaaatatca aaaaaacttt
    18661 caataaaggg ttgcaaaaat ctggaaaatt aggtattata actaagtcag gttgatacaa
    18721 acctttcgga ggagtagcga agtggctaaa cgcgacggac tgtaaatccg ttccttaggg
    18781 ttcagtggtt cgaatccact ctcctccatt tcattggggt atagccaagc ggtaaggcaa
    18841 gggactttga ctccctcatg cgttggttcg aatccagcta ccccagtata ccacaatcgt
    18901 aagctctcgt tagttcgaga gctctttttt tttgctttgc taccttgttc cttgaagatt
    18961 ctgctgaatt aagatagagt taagttaact acaagaattg gagggatcca catgaaatgg
    19021 aaagaaaaag tagacgaagc agctgaaaag ttatatgatt tggtcaaatc agaaaaatac
    19081 aatattgaag tgaatattcc gaaaaaaggc ggaaaagcgg tccaggtaaa atcaaaacgc
    19141 ccaacgaatc atacaaaaaa atggatggct aaaaaccgct aataataaca aaagatccat
    19201 aagtcggcat tttcatcgat tttagggtct tttgttattt tctaggttga acatatctaa
    19261 aaaattgttt ctgacaatag gagtagggag aacgatgtgt gtctaaatcc tctgttgatg
    19321 gatagaaacc tttatctttt tcagttgtga atcaggtata taccagttat aatagaagaa
    19381 acgaaaggac gggtgtacgt tatgtcgaat caattacctg tatatataca gattcatgac
    19441 caattaaaaa atgaaatcga acaaggtata tggaaagtcg gagaccgtct tccttcagaa
    19501 agagaattag cagtgaaatt caatgtcagc cgtatgacgt tgagacaagc aattcaaaac
    19561 ttggcagatg aaggaatctt agaaagaaag atcggttcgg gtacgtatgt tgcgcgagaa
    19621 aaagtacaag aaaaaatgtc tggtgcaaca agttttacag aaattatgga atcacaaaat
    19681 cgagtaccgt ctagtcggac aatctcttat ttcttgactt cgccaagctc aagtgaaatg
    19741 gaaaaactaa acttgagtaa agatgacacg atcttgcgaa tggaacgtat tcgtttcgca
    19801 gatgatgtcc ctatttgttt tgaagttgcc agtgttccac aaaagctgat cgatggctac
    19861 agcaaagcag agattactga atcgttgtat cgcacgctag aagaaaaagg cgggaaaaag
    19921 atcggtccag cgaatcaaac gattactgct gtgttggctt ctgagaaaat tgctgactat
    19981 ttagaggtaa aacgaagaga tgcagttctt cgtttgcgcc agatttctta ttttgaagac
    20041 ggtacgccgt ttgagtatgt gcgtacgcaa tacgtaggaa atcgttttga gttttatttg
    20101 gaaaaataaa aaacaggtga tccctgattc cttattagaa ataaaagaga atcagaatca
    20161 cctgttttta tatatgatca gttatcagtt gctcgtgata agaaacagaa ccctcttcaa
    20221 attggacttg tccgttcagc agctcaatga tggattcttt aaagtggtta gcactttttt
    20281 catctacagc acagatgacg ctaacttgat cagtatattg gatttctttt ataaaaaaat
    20341 gttggagttc aagataattc tgtaattttc ctagttgcgg atacgtgatc atcacagaga
    20401 tctcctgttg caaggtacct tctacgattc caatgtcaga caatgcttga gagaccgcat
    20461 gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt tcctccaaaa tatcgagtga
    20521 ctactgcagc tacattgatc agttgatttt ttttgaggac ttccaacatc ggtacgcccg
    20581 ccgttccact tggttctcca tcatcgctgc ttcgctggat ttcatttttt tctccaatga
    20641 tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt ttttatttct tgaatgaaat
    20701 tttttgcttg gtcctctgat tctattcgtc taagagaaca gataaagcgt gattttttga
    20761 tctcaatctc actttgtcca ttttctttta ttgtacgata gcgctcgatc atcttgtcac
    20821 ctcttttttt cattttcttt attcagtata aataaagttc taggttgttt caacactgta
    20881 acggttgcgc aattgctgta gtatgcgata ataaagacaa aggcaggtgg atctggggat
    20941 gcaggaacga atgaaaaagt acgatgcaat tacgcattat ttgaagaaca atggcggctc
    21001 tcaagtaacg ttgaccttta cccaatttga cgaactgctg tttcccagta acggacttcc
    21061 caaaacagca agggaaagta cagattggtg ggcaaatgat tataaacacc ctgaaaaagg
    21121 tgcctatggc tggataaatg caggctatga agtagttgtc atcaatttgg acaaagaata
    21181 tgtcgtgttc aataaattag tgaaatctag ttggctattt gattaaataa accgcgttta
    21241 gaaaacgttg acaaatttct ttctttttat ataataaagt aacagacgcg aagaaaacgt
    21301 ttgcgtagtt tgttttaaaa aggagagaaa aatatggcta aaaaaacaat catgcttgta
    21361 tgttcggctg gtatgagcac aagtttacta gtaacaaaaa tgcaaaaagc tgctgaagca
    21421 aaaggaatcg actctgatat tttcgctgtg tccgcttctg acgcagataa caatcttgca
    21481 aataaagacg tggatgtgtt gttattaggc ccacaagttc gctttatgaa agctgatttt
    21541 gagaaacgtc tggaaccaaa aggcattcct ttagatgtca tcaatatggc tgattatggt
    21601 atgatgaatg gcgaaaaagt gttagatcaa gcaatcgcgc tcataggtga ataaatagaa
    21661 cagtataact atcgaacaag cgatccaaaa aatagatcgc ttgttttttc gtatattttt
    21721 ttagggcgaa taatatgaat catttttggg gtagaaggct tctgacgaga gatattaaag
    21781 caatagactg tgaggaggta aatttaccaa attatgagaa gatcgcagca gtggaacgtt
    21841 tgggcaactg gattctttgt catcgctgtg gaatgaggac gtccgtcatt gttggacagc
    21901 ttgcagatta cggttatttt tgtacacatt gtctatattt agggtgatgc gacactcaac
    21961 aagatttata tttgtttgat cagcctgaag caaaacctcg agaagtcgtc tttttttgga
    22021 caggacagct tactgaaaaa caaacggaaa tcgcaaagag aatactttgc cattcagaaa
    22081 aaagacatca tcttatttgg gctgtaacag gtgcagggaa aacagagatg ctttatccta
    22141 ttttagtgga aactttaaaa gctggaggaa gagtagcgat ttgtacaccg cgagtcgatg
    22201 tttgtaatga attgttttta cgctatcgtt atgtttttcc agaagaaaaa atcacgttgc
    22261 tccatgggaa tacagcaacg gcttatacct ttacttcttt tgttatctgt acgattcatc
    22321 agctataccg tttttatcgg tcctttgatt taatagtgat cgatgagatc gatgcgtttc
    22381 cttataacgg ggatgctggg ctagctcacg ctgtgcaaac agcaatcaga tcaaacggcc
    22441 gattcatcta cttaagtgcc acgcctgatg agaagttact aaaggaaatc aagcagacat
    22501 ttgagctcca caaacttgcc ttgcgctttc atcggcgctt gcttcctgaa cccgtgcttg
    22561 ttttcatgaa taattggcga caaaaatgtg gaaacaaaaa gaaaatcaac tctttggtgc
    22621 ggatcattca gcaactaatc aaagaaaacc gtatcctttt attttgtcca agtattacga
    22681 tgatgcatcg tttaaaaatc acactgcaag aagcttttcc agaatatgag ataacagatg
    22741 tttcttcaca agacgatcaa cgggcaaaga aagttcagaa aatgcgagaa gcaatatacg
    22801 agatactgtt aaccacaatg atcctagaaa gaggagtcac atttgaaaga atctctgtca
    22861 tcgttttagg agcagatcat ccagttttta ctaaatcctc tttggtgcaa atcgcaggaa
    22921 gagctgatcg taaaggtcct tatacgaaca gtcgagtcta ttttttctat gaagaaaaaa
    22981 cttcagctat tcgaaaagcc tgtcaggaaa tcaaagaaat gaacgaagaa gggaagcaga
    23041 tgctgtgaga tgtagttgct gcggtaaaga catcattcaa aatttaacat tcagcgagat
    23101 catcggagtg tcgaaatcta gacttcctca gacatgttca gtttgccgcg cacaattcca
    23161 tttattagaa gcaaaaggtt gtgcaggttg tcaaaaacca atccatttga aatacgagga
    23221 gacgaaaaag aaagcaaata ctagtttcgt ttactgcaac gactgtcaag aatggaagaa
    23281 gaaatatcct gcatatgatt ttcaacaccg cgcctttttt acgtacgatg aaaagatgca
    23341 ggcttggatc aatcggtaca agtttatggg agatatccga ttagcaggga cttttgcata
    23401 taaatggact cagataaaaa aagattaccc ggcatatatt tattgtccaa tacctttatc
    23461 tgaaaaaagg tggctagaaa gaggattcaa tcaagtaagc gagatgttga aagcagcaaa
    23521 aattcctttt cagatgctgc tcaaaagaga gcaacatctg cttgctcaag caaaaaacac
    23581 gcgaaaggaa agattggaga tgcctcagcc ttttgagctt gccgtacctc tttctaagat
    23641 acaacataaa aaaatattga ttattgacga tgtctataca acaggacaga cgctttttca
    23701 tgcagcagat tgcttgcaga acgcacagcc gaaaatcatc cgaaccattt ctttggcaag
    23761 atgaatcaga tgtggaaatt tgatagcgat ttaatttgca aataacctag agttcgatat
    23821 aatagaatta agaagaggga aagagtggtc cttctccttc ttgattgtgg tagacgaaag
    23881 gggtaatttt tatgtttaga tataatgtac gcggcgaaaa tatcgaagta actgaagcga
    23941 ttcgcgacta cgttgagaaa aaagtaggca aactagaacg ctatttcagt gacgcacccg
    24001 atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa aacagcaaaa gtggaagtaa
    24061 cgatcccatt accttatctt gtattacgtg ccgaagaaac ttctccagat ttatacgcaa
    24121 gtatcgattt ggttgttgac aaattggaac gccaaatccg caaattcaaa acaaaaatta
    24181 atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc tgctgcttta ttcaatgaag
    24241 aggacgaaaa cgaaaatgat tctgaattag atatcgtacg tacgaaacgc ctttcattga
    24301 aaccaatgga tagtgaagaa gcagtcttac aaatgaacat gctgggacat aacttcttta
    24361 tttttgaaga tgcagaaaca aatggtacaa gtatcgttta ccgccgtaaa gatggaaaat
    24421 acggactgat tgaaacagac tgatcagcct tacttaaata acaacgaaaa cttgcagcag
    24481 ctactgacag ctgccgcaag tttttttgag tttcctcctc ttttagatga aatatgaata
    24541 a
//
LOCUS       DACJGB010000044        23679 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.044, whole genome shotgun sequence.
ACCESSION   DACJGB010000044 DACJGB010000000
VERSION     DACJGB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.044"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(54..788)
                     /locus_tag="IYI34_001787"
     CDS             complement(54..788)
                     /locus_tag="IYI34_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAR0559654.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IYI34_001788"
     CDS             1115..1525
                     /locus_tag="IYI34_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAR0559655.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IYI34_001789"
     CDS             1518..2219
                     /locus_tag="IYI34_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAR0559656.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IYI34_001790"
     CDS             2219..3805
                     /locus_tag="IYI34_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR0559657.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IYI34_001791"
     CDS             3824..4045
                     /locus_tag="IYI34_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAR0559658.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IYI34_001792"
     CDS             4042..4803
                     /locus_tag="IYI34_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAR0559659.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IYI34_001793"
     CDS             5183..5692
                     /locus_tag="IYI34_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAR0559660.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IYI34_001794"
     CDS             complement(5767..6306)
                     /locus_tag="IYI34_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAR0559661.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IYI34_001795"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IYI34_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAR0559662.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IYI34_001796"
     CDS             7373..8170
                     /locus_tag="IYI34_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAR0559663.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IYI34_001797"
     CDS             8198..8833
                     /locus_tag="IYI34_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAR0559664.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IYI34_001798"
     CDS             8853..9626
                     /locus_tag="IYI34_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0559665.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IYI34_001799"
     CDS             10034..10831
                     /locus_tag="IYI34_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAR0559666.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IYI34_001800"
     CDS             10809..11222
                     /locus_tag="IYI34_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559667.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IYI34_001801"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IYI34_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAR0559668.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IYI34_001802"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IYI34_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAR0559669.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IYI34_001803"
     CDS             15999..16559
                     /locus_tag="IYI34_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAR0559670.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IYI34_001804"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IYI34_001804"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAR0559671.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="IYI34_001805"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="IYI34_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAR0559672.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="IYI34_001806"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="IYI34_001806"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAR0559673.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="IYI34_001807"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="IYI34_001807"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAR0559674.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="IYI34_001808"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="IYI34_001808"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAR0559675.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="IYI34_001809"
     CDS             21906..22484
                     /locus_tag="IYI34_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAR0559676.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="IYI34_001810"
     CDS             22536..23018
                     /locus_tag="IYI34_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAR0559677.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="IYI34_001811"
     CDS             23135..23509
                     /locus_tag="IYI34_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559678.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7734 a         4275 c         5075 g         6595 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagtttttt
//
LOCUS       DACJGB010000045        23192 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.045, whole genome shotgun sequence.
ACCESSION   DACJGB010000045 DACJGB010000000
VERSION     DACJGB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.045"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(361..987)
                     /locus_tag="IYI34_001812"
     CDS             complement(361..987)
                     /locus_tag="IYI34_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559679.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1282..1617)
                     /locus_tag="IYI34_001813"
     CDS             complement(1282..1617)
                     /locus_tag="IYI34_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAR0559680.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1604..1888)
                     /locus_tag="IYI34_001814"
     CDS             complement(1604..1888)
                     /locus_tag="IYI34_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAR0559681.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1944..3467)
                     /locus_tag="IYI34_001815"
     CDS             complement(1944..3467)
                     /locus_tag="IYI34_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAR0559682.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3460..4635)
                     /locus_tag="IYI34_001816"
     CDS             complement(3460..4635)
                     /locus_tag="IYI34_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAR0559683.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4639..4824)
                     /locus_tag="IYI34_001817"
     CDS             complement(4639..4824)
                     /locus_tag="IYI34_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559684.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4790..6484)
                     /locus_tag="IYI34_001818"
     CDS             complement(4790..6484)
                     /locus_tag="IYI34_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAR0559685.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6481..6954)
                     /locus_tag="IYI34_001819"
     CDS             complement(6481..6954)
                     /locus_tag="IYI34_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAR0559686.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7023..7178)
                     /locus_tag="IYI34_001820"
     CDS             complement(7023..7178)
                     /locus_tag="IYI34_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAR0559687.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7312..7692)
                     /locus_tag="IYI34_001821"
     CDS             complement(7312..7692)
                     /locus_tag="IYI34_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAR0559688.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7696..7911)
                     /locus_tag="IYI34_001822"
     CDS             complement(7696..7911)
                     /locus_tag="IYI34_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559689.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7914..8321)
                     /locus_tag="IYI34_001823"
     CDS             complement(7914..8321)
                     /locus_tag="IYI34_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAR0559690.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8587..10062)
                     /locus_tag="IYI34_001824"
     CDS             complement(8587..10062)
                     /locus_tag="IYI34_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAR0559691.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10052..10900)
                     /locus_tag="IYI34_001825"
     CDS             complement(10052..10900)
                     /locus_tag="IYI34_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAR0559692.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10937..11299)
                     /locus_tag="IYI34_001826"
     CDS             complement(10937..11299)
                     /locus_tag="IYI34_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559693.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11599..12223)
                     /locus_tag="IYI34_001827"
                     /pseudo
     CDS             complement(11599..12223)
                     /locus_tag="IYI34_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12220..12531)
                     /locus_tag="IYI34_001828"
     CDS             complement(12220..12531)
                     /locus_tag="IYI34_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559694.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12575..12868)
                     /locus_tag="IYI34_001829"
     CDS             complement(12575..12868)
                     /locus_tag="IYI34_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559695.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13062..13709
                     /locus_tag="IYI34_001830"
     CDS             13062..13709
                     /locus_tag="IYI34_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0559696.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13770..14915
                     /locus_tag="IYI34_001831"
     CDS             13770..14915
                     /locus_tag="IYI34_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0559697.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(14996..15301)
                     /locus_tag="IYI34_001832"
     CDS             complement(14996..15301)
                     /locus_tag="IYI34_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAR0559698.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(15326..15757)
                     /gene="ruvX"
                     /locus_tag="IYI34_001833"
     CDS             complement(15326..15757)
                     /gene="ruvX"
                     /locus_tag="IYI34_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAR0559699.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(15754..16020)
                     /locus_tag="IYI34_001834"
     CDS             complement(15754..16020)
                     /locus_tag="IYI34_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAR0559700.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(16275..17000)
                     /locus_tag="IYI34_001835"
     CDS             complement(16275..17000)
                     /locus_tag="IYI34_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAR0559701.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(17361..17431)
                     /locus_tag="IYI34_001836"
     tRNA            complement(17361..17431)
                     /locus_tag="IYI34_001836"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17397..17399),aa:Gly,seq:ccc)
     gene            17548..18300
                     /locus_tag="IYI34_001837"
     CDS             17548..18300
                     /locus_tag="IYI34_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAR0559702.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18316..18795
                     /locus_tag="IYI34_001838"
     CDS             18316..18795
                     /locus_tag="IYI34_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAR0559703.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(19046..20626)
                     /locus_tag="IYI34_001839"
     CDS             complement(19046..20626)
                     /locus_tag="IYI34_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAR0559704.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(20619..21362)
                     /locus_tag="IYI34_001840"
     CDS             complement(20619..21362)
                     /locus_tag="IYI34_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559705.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(21538..21927)
                     /locus_tag="IYI34_001841"
     CDS             complement(21538..21927)
                     /locus_tag="IYI34_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAR0559706.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(22008..23134)
                     /locus_tag="IYI34_001842"
     CDS             complement(join(22008..22906,22906..23134))
                     /locus_tag="IYI34_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAR0559707.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT         6758 a         4658 c         3774 g         8002 t
ORIGIN      
        1 ctgtgaagtc aacgttttga cctgattatt agagaaacaa atggcaaaat agttcttcat
       61 atttcgccgt ttttcgagcg aatgccgagg ggcagctgat tgaacatcgt ttatttggaa
      121 aaagggattg tgacaagacg tttgtcacaa tccctttttt tatatcttat taattcattc
      181 ttttgttcta ttcttcttct tccgccatga atgagaaacc catatcaaag aactataaag
      241 gttatcaaaa cagcgtttat gactaaatga attagagtta tctatcacta tttttcaagc
      301 gatttaaacc aaatggcata caatatggca tacaaaaact tcaataagcc gtgggggttt
      361 ttaaatttct tcttgttctt taaaatcaat gagtggagct acataatttg aatccacttt
      421 ttcaaacgtc actttttgca tcgacgaatg ttttctttcc caagataacg taaatagatt
      481 tgactctgga cttataaagt ctacttcacc ttctagtatt actttcttat gcaaatattc
      541 ctctaataaa tatagacttg ctaaagcata caacacattt tccaaatttg cttccttata
      601 atttgaagaa cgaccatgtt ttactctgtt atatattttc caccaatctg gtgtatcttc
      661 aggccaccca tctccaaaag gttttagtga agtcaatgtt aaagaattca taaccctaac
      721 accattatcc aaacacatta attcgctaga tttaataaca gctttaaaat cagttatgct
      781 tttattgcta ctgctatcaa atccgcaaag ttctttgaaa ataatctcac actccgaacc
      841 aatcgctagt agtagttttt gatattcaat agagaatgtt ttgaaattat tctcatcgag
      901 catcacaaaa tctgtggtat taataaactc attttctaaa tgtagataat acttccaata
      961 aaaatttgta aactctgctt tgtccattaa atcacctctt ttgaattata acaaaaaaaa
     1021 cacgtccaca acagacgtgt tctttgattt ctacacaatc agcttgccaa aaccatccaa
     1081 tcagtcctag catgcctgta ttaaaattga acgttcatca tctatcatct ttcttagtcc
     1141 agcatgtttg caaccacacc tacaattttc agtccaacaa tagatttcga ctagctaagt
     1201 cttgcaaaat tcaggcagct aacccttcat ttactacctt aattatacta aatacccaca
     1261 acgtttacaa gtgtaatcat tctaagaaat ctgtttagct atgatcgttg tataatcttt
     1321 aaacgccgtt ccttcattga ttgaaataat tttataactc ttgtttttcc aagctacttt
     1381 catatcgttt gttagttcat aatcttgatc gtatcgaata ataaacgtca acgttccttc
     1441 aagaatggta cctacactag caagcacgtc ttttagcatt tgtgaacgca cattagccca
     1501 acattcaaat acagtttctt cactatccac catctgccca tcttcatctt ttacccgttt
     1561 tgtcttttta aacttgactc tctcagtcaa ttcatttaca ttcttaatga ttggcataat
     1621 aagcacctct catttgttgt actaatgctt gtaccgttac tggtatccgt tcgctgctgg
     1681 cttcttgacg gttcaaatac caatgttgag taagtaatga caccgccaaa tcaaattgct
     1741 gataattatc catatcttta tccgttgcgc tactatcaat agcacttttt atatattcgc
     1801 ctgctgtatc aatcaattgt tgaataaagt catcatcaat agtgtgatcg atccgcatac
     1861 ttttttttat gctatccaaa attaccattt tatctctcct aatgaaaaaa aggcagtggg
     1921 ataaaccgcc gcccaatatt tttctatact tttggtgtta caactggcgt aaactcaata
     1981 tacactgaag cattttgatc aattttctta taatcgttgc gaacgatcac tgcaagtcct
     2041 tgtgagtagt aatcgaattt ttcccattga gtcgttactt gatttctacg agcaacaaag
     2101 atagattgtg caatatcccc cataatcata gggaatgttc cagctttagg attggcaaac
     2161 aatgtatctg caatcaatac tactggcata ccgaataatg atttaccact aggtgctgtc
     2221 acatcgggtt gtaaaatgta acgtccatct gaatctttca atgtatctag gtggtaatat
     2281 ccgctttggt ttagaattac cattttattt aatgctgggt ctaatgccac attgtatagt
     2341 tgtttcaaat cgtccaacgt agcagccgtt ttcttagtga atgtttttaa taaatccatg
     2401 atatgcttat tgtcagtgtt ttctaccaat ttcgctaact gatctttgac ctcttgtaca
     2461 atattcactg ctgaatcttc cacaacctca tttgataagg caatcttacc agcacgagtt
     2521 tctactttat aatcaactga agtaaacatt tcagcgtcaa tatcaccaat ttcagcaagt
     2581 tcagcttttg ttgctaacac tgcttgttgg ttagttgcta ctggatattt accttgtcca
     2641 tttgatactg tttttactgt tgcatattga gctaagttat aatttgaacg tttcaaatca
     2701 aagacttcac cgattacttc ttcgggaaca actgccgctg catttacagt agtgactcca
     2761 tcacgcactt cgccttgtga acggatatat tcttcatacc cacgcaattc tgttttttcg
     2821 ttatcgatta aagttttttc catgttatta ctctcctttt gtgctttaga agtggattct
     2881 aagctattgt ttgtttgttt tgcttggttg ttactcataa acgattcgta agaacgctta
     2941 tttacttgga catttgatga atcataagcg ggaacggtca cgacactaat ttcattaagt
     3001 gctttgattt tatctattga tcgttcaatt gtgccatctt cttttttgtc gaatgaatct
     3061 aatccaagca cgaacccaaa ggacatagaa tcaacaaccc cttttgagat attctcatac
     3121 acatcatttg aataggtcgt gtcggttagc tctgcttcaa aatgtaatcc tgtatcatcg
     3181 acattcaatt tcaacgtacc tgctttgaca ctagctagtg gctttgaata atcgtgtcca
     3241 tataataaga acacatttga taaatccacc tcttttaatg cttcgggtgt aatcacttct
     3301 acaaagccgc ctaaatcttt tgacggttgc ccgaatttca acgcatagcc tgaaatagtt
     3361 ttgccgtctg ttggttgttt ttcattttct ttctctactt tgggagaatc ggctgttagc
     3421 tcagcttctt ctgtcagccg tttttccttt tcttcttcat tattcaactg gtgtaactcc
     3481 ttcctgttct tttaattgat atttctccaa cgtatctaaa tacgtaaagt tcaaacttgc
     3541 tagtaaacga tcgccaccat ccatgggggg tagccccatt ttcgaccgcc cctcattaat
     3601 cgtgagaagt gaccctttga cctgatccaa tacatttttt acttttgttt caggatcagt
     3661 ctctagtaac tgatcggaat taaagcggat attggtttca agtttcgtat ctaattcact
     3721 cacaaacaca ttaaagtaat gaatcagcgt actttggata tactggagat tactttgaac
     3781 ggtgctagaa tgttcatttt caacgcctaa acgctcaatc ggtacaccaa acgccttggc
     3841 aatttgtttt gtgttccaat cattggaatt gataagcttc aaaacatcgg tgtttatttc
     3901 taatgttttg tagtccattg tttcatcaag aatgatcgtt ctaagagcat tatctccact
     3961 acttgaaccg ttggcttctt caaacttttc acgaattgcg ttctttgctg atccgtctaa
     4021 atctgactta tgaaccttca aaatacctga cccactgaca ccacgagtaa agaaattgtg
     4081 caatgtgcgg ttgccagctt gttgaatttt cagttcatca cgtaacgcat aaagtggtgg
     4141 taatcctgtt aaaccatctt gggtgaaata tttgaagtgc aaaatatcgc tagacttcac
     4201 acgtcttttt ttatcaccaa tctgataaga caacgtacca ttatctaatt gagtaaccgt
     4261 tacacttgag ttaggtaata agtgaatttc ttctaccttt tcaccgttgc gtttaatctc
     4321 tgcaaagcta ttaccgttta gcaacatatt gaccgccaaa gcaaacttta aatgccaacc
     4381 atccatttct gaattaggtt tctcgtttag taacttcacc agttcattat cagcttgtgg
     4441 catattgttt ttgaccaatt gaatcggact tgaagcaata tcactagcaa taattcgaac
     4501 ggctgcaaac acatcactat ttcttaatgc acgaacgctt gtaaagctgg tggtgtaatc
     4561 atccgattgg attgatacca catgttgtag gaaaggatct cccgttgtat tttgtctcgc
     4621 tttaaaaaat gccatctttt aacctccctt ctgttctgta tttagcatga ccgctacccc
     4681 aataagcact atgcccagcg ctaacaagcc atagtagaca tttgttagta aggtgatagc
     4741 aaccaacaca cacaccaatc caagaagtaa aagaacggta tgaatgtaat tagaaagaaa
     4801 attcttcact tgtataaaat tcattgtctg ctttcgctcc ttccgttgtg gtgaagtaat
     4861 ccattgcaaa cacataggca ttgattaatg cagctattgg atcaattttg ttactgttct
     4921 ttgatttatt aatctgaatg ccgttgttat ccgtttttaa aatagcgttg ttcactgcat
     4981 gggtgagaat cgtattttta ttgtgaataa cgttgccttc ataaacttgt tctctaaaag
     5041 ttcgggtcgg aacgtttaga gtaatcgttc cttgtcttac ttctaacatt gggtagttgg
     5101 ctttttcagc tttactgata agcgaattag cgttatacgg gtcataacaa atccccatac
     5161 attccaaatc attttcttga atcaaatctt gaataaactg aaatacttgg tcatagtcca
     5221 cgattccact ttctaattga gtaatggaac actcaccagc tttttctagt tctcgataag
     5281 gcaaaccgtc gcgcttttct ttgtcttgaa gcccatattt ggtggctaca aagctatgtg
     5341 aatcacaata aagttcgccg ttatctagcg gtacgatcca tgaaacgcta gttaaatcat
     5401 ctgttttaga taaatccact ccgatataaa cgggcttgcc tgtaatatct tgtggttcta
     5461 cttcggttgc ctgccagtct tcagcaatca tatagctgtc ctcactcgct tgtctccata
     5521 aattgaagtt tttcaccaat acagaattaa ggttattttg tttcaaagca aggttcacat
     5581 catcttgaat ggttggaatc attacttttt tgatttcttc actttcaaaa atcggattgg
     5641 ctttgatcca tttctcttga tcgtgaattt cttcgggatc gtccaattcc catatcgcta
     5701 taaagtacct gtcggcttgt tcttcgcctt ttagcacacg atctaacaat aagtattcct
     5761 cgtacatagg aacgtttaaa tcaagcccag cggtactaat aacacacaat aagccgttct
     5821 tttgttgggt cattcctgac ttaatgacgt tgtacgtttt acgagttttt gcttcgtgcc
     5881 actcgtcaag aattgcggtt gttggtgcaa acccatctag cgtgctggtt tcactagcca
     5941 gtgccatagc gaacgaatta gagggcaagt gctgaatctt agagttcata attttcagtt
     6001 gtggtcttaa aaacttacta gacttgacca cgtttctaag tgaattagac agcatatcat
     6061 agcccaattt agcttgtttc aaagcgttac ttacaaacaa tacttgcctt gcttctgctg
     6121 gttcttgttc cataatcaac gcattggcag ccatgccact tgcgatatac gttttcccat
     6181 tcttacggct catactggta aacgctcgat tgaatcgcct gtaattccct tctttagtac
     6241 gccagccata aagactgcct agaatgaatt tttgaaatcc taacatcttg attgctttac
     6301 cgtccgtaga tggtagcaac tcaatgaatt taatcgcttt ggtggcttgt tcttcatcga
     6361 acacataagg gaagctatct gacttagaac gctccaaatc gcttatatga cgctcacacg
     6421 cttggataat tttcttgggt gcaatcagct tacctgatag gactttctca atgtaatcat
     6481 tcatgaattc atcaactctt tgaacgggtc ttcgggttca tcatttttaa ccttgttcaa
     6541 agctagtttt gatcgtgatt ctaaggtcat acctagtgag gtggcttgtt ttttcaaatc
     6601 actcatagct tgtgattgaa cccgaaccgc tggattggct ttctttactc cgttttcgca
     6661 tgtgataaac gtacctgatt ttcttatctc ggcttcggct gttttcattc gagcaaaggc
     6721 taaacaatag gctaccaatg aaccataatc tgcttcactc aatggataat ctttttttaa
     6781 tgttggtacg atacgattcc attcacttct agcgctcgct gccatccagt cagggggcgt
     6841 gatatttatt aatggttgct gcttgaacaa ttcttctcta gcgtccacac ggattgcttt
     6901 ttcttcactt gatatattgc cttttgtatc gtgtaatatt ttagggtgtc ccatgttgtc
     6961 ctcctttcag acaaaagaaa aaggaacgta agcaattaag ctcacattcc ttcgtttttc
     7021 gatcagaatt tttctttatc taatttatct atataaatga tttttccgtt ctgcatttga
     7081 atggtcactt gaccaaaatg aggtaactca atggtgcgct gtttccctgt tttatcagta
     7141 ataacaattg tatttttcaa taaatctcct actttcatta tatacttttt cacttgtgat
     7201 tacaagtgta atcgttattt gtaattaccc ttatgccttt tcacaacggt tatttgtacc
     7261 aaaatgagta ggcatcgatt gtcagtaagt ttcacactat gcccccttaa ctcaaagggt
     7321 actcttttct cgtctttgtc tttcattggc tgtcttggaa ttatggtgtg catggcataa
     7381 agaacgaaaa ttagtttcat ctaaccgtct attccaatca tcttttagtt caattttgtg
     7441 atctacaaca tcagctttcc ttatcaagcc ttcttctagg caatcctcac atactggatg
     7501 atttaaccga tacaagtatg aagcctttcg ccatgttcta ctttgataga attgaaaata
     7561 tttaccttcg ctttgctttc tttcttcacg cttaattgcc gtttgctttg ctttatgctt
     7621 cccacaatac ttctgcctat aatcaatcaa tgttttacat ccagcatgat tacattgttt
     7681 tttaactgcc atagattacc aaccaaagcg atctcgttcg tcagaatcat cttgttccca
     7741 ttttgatttg tattcaatat aaggcgaaac tatcttcaag cacgttccgt ctttaaaatt
     7801 aactttagtg attttatctt cctcaaaact aataaagtct acatcttctt gagtgtttga
     7861 atactcaatg tgtttctcac catcgaagta tctaatactt tccatatgca taattattct
     7921 acctccaata aattagttat caattcatca tctgttagta gcaaagccaa gaacttatca
     7981 tggatgttta aaaacaaatt atcctcattc gatgtaagct ctcgttgtct cgctcgatca
     8041 tataggttaa gcagacaatt aaattcatcc attagctcac ctgtatcatc atagttgcca
     8101 ctatctacca atgatcccac tactagcgat tgacaccacg ctactgactc ctgcagccaa
     8161 tcaggttctt cttccattat ctttaatact tctggatcaa atgtggaata atcttctgtt
     8221 accaatccca cactaaatgc cagcgtgttc attagaaacc gatccatttc atctgtatat
     8281 tgaaagttcg gtgctttgtt tgttgaaata atattgagca tttactttcc tccttgattc
     8341 gtaacacgtg gtaacaccta cgtaacaggt acccgttacc actcaatccc aacggggaca
     8401 agggttctag cgttttggta acaggtaacg ggtaaaatcc agtaacatta ttccccagca
     8461 tattagctaa ctatttcaaa agttttaagg aaataaggtg ttaccccgtt accaaattgc
     8521 tgtatccctt ggggcagtaa gggtaaacta gtaacgggct aggtgttact gacccgttac
     8581 tacccgttac tttttgtact ttccagctcg atttttattt tcataggcat ttgacttatt
     8641 gtaaaatcct cgtcgtttat ttctttttgg cagttgcttc cgttcccaac caggaatact
     8701 gctaataatc aaggcaatct ttttagtttc tgcatctgat cgaccattca ataaatcctt
     8761 tgcttgttta tcaaaaacag cttccaaaat atcagcggtg agtacgctat caactaaata
     8821 aaactcttgt tgcatagtag ctccattata gaaatgctgt aaatttttct cattacattt
     8881 actgtcagga tagttctgat aataagaacg tctcgcccac gctggagctt cttcccattt
     8941 atacgggatt tccatttcca aatatttagt gattgcttct ttaattggat tctccatctt
     9001 ggcatcctct tgatagtctt ttgcaatttc taagacttct ttatcttcat cgggactaaa
     9061 atagattcgt tgtcctttat caaaaagcac ctttgcttca gctaacactt gaagaaaata
     9121 ttcatccttt gttttaaacg gattcatctt tggcttattc ttacaaggca aaggataaaa
     9181 acggcggttt cctgtatcat ctttcaaata ctgcccatca ttcgacgttc caataaatac
     9241 acattgcctt gcccaagcta tgacattccg accataagga ggtctaattt tatcgatctt
     9301 ggcactgatg aagtttttta tctgttcaat tttagttttg ttcattgaag ataattctcc
     9361 aagttcaata attacattgc cttgaagctg catataatca tctttctttt cacctagcga
     9421 atctaatgaa tcggtgaaaa aatcatccgt gtaaagtaat gctggaacgg tactcttacc
     9481 aatcccttgc ggtccatcca aaactggaac gatctcaaac tttattcctg gtttatagat
     9541 acgtgcgact gatccgacta tccaacgttt tgttacttct cgtgtgtaaa ggttgtcgtc
     9601 tacccctaaa aggtcaataa acagtgtttc cagtctaggc tgttgatccc atttaacttt
     9661 ttcgatacgt tcttttactg ggttgaaaac attctttcta gccacattta ctactgcttt
     9721 gtgtattttg ttgtcatcaa aaactacatt gtagttttct tcaatatatg ccgttaaggc
     9781 atcctcaaaa ctgtcttccc attctccagc ggtacgattg atccaaggag aatcttttaa
     9841 taggtgaatg tatccgctaa attcgttata agcaattgtt ccagctaaat tgctatcatt
     9901 ggttaaaatc aattctgcat tcacaacact gttactttta atctgatcgt ctttattgca
     9961 tcttaatcgt gacatccagg ttttcaattc aatgatattg tcttcacgct cgctggcttc
    10021 gggaaagtct aaaatactat ctaatttatg cttatgagcc atgagctttc cccctcaaat
    10081 cttttttaat catcgatcga ataatttgat tcacttcttt atcttttagc ggtggttcat
    10141 tgtacgcatt tgctgtataa atcaattctg ctaaagtttg attatctaca ccgtgccata
    10201 gtaaccaacc gcataactta gttaaatgat cgtttctact accttcgcca gcgccttgaa
    10261 caatcatatc taattttttt gctgtccctg tttttgctac gggtcggtat tgagtcttta
    10321 tttcttgcgg tttttttagc agtaggttca ctagccaatc gggagctggt gcaatattat
    10381 ttagagatac gttttcaata ggttcatatg ctctcccaac acttcttgaa ataatcatag
    10441 ttggcgctat tggaactcct ttagtgataa tatcaatccc gcttaaactt ttacctttat
    10501 catcacgccc gaagatatca gcaaatgcac ctgtttcttg ttggataatt acaccatcag
    10561 gaattttaaa gaagaaatgg attcctttac ctggtgtgat ttccgtataa gtagaaggaa
    10621 atggatcata cattgaagca agttttttat aattctcaac gccgtttcgt gtttcagaat
    10681 gttgatctag gtcaactacc accatttgat gatcttcaag cattaaccca atatttctag
    10741 ttgcatcttc cgtccaccat tcattaattt gattagaatc gttagttgca tcattccaac
    10801 cactagagcc ttttattgga attttagttc caggagataa gggataaatt gaccatcccg
    10861 acttttgtaa ttccgttgct gcttgataat tatttgccaa taaacctctc ctcctttttc
    10921 aaaatactgg tataaatcat tcagacacct ccatgagctt caactgctca agttcgtatt
    10981 tcaacccatc ccgaacaaat tcagcacctt ttaattgatt ctgatacttt acccatgtat
    11041 taaatgtttt cacccttgct tcttgcatat cagtaatgtt taaccatgcg gttaaaatct
    11101 gattttttag ctcgttgatt tctcgacaaa ttattttgtg ttctgcatgt tttttgagta
    11161 catacaaaac tgttagtttt tcattgaatg aatttccaaa gtaggttttc ccgatattat
    11221 tttctagttc agcgatccct tgattaattt cctctaattc aaggctatcg agttggttta
    11281 atagttttgt tttgtacatc tacttatgcc ctccagttct taattgatag tcgtactcgt
    11341 cataagcagt gaagagatat aacgctaatc ctgcaaagat agctattttt gcaattaatg
    11401 gtaataagcc gaaaattccg ccgattaaaa tgcttcctat aattaagttt cgtttcataa
    11461 tggttccctc cgatttttta atatggtata atcggaacaa agaatatcgg gttttatccc
    11521 aattaagttg cctttatcct gtccgccaag actgttatgg ataaggcttt ttctttgttc
    11581 tcgatcatgt taatgctcct aagttgtctg caattcagca gaacgattat aaattttctc
    11641 actcactgaa aactgcaacc catatttttc tttaacagtc attaattcta ctgtctcatc
    11701 taaaataggt tcacgatcaa ccaacatttc aggagtcata tctttctttt ttaccatttt
    11761 ggaatgacca taaatagttg atacagcttt attggctatc acattcgctt taatgtagtc
    11821 tactggtttt ggttcagtaa tagcgtggct taatttagtc attgcttctt tttggtgttc
    11881 tttgtctagc atgcggaata cttgaaagcc ttcaagtcct gtggactggc gtagttcttt
    11941 gatgatgtcg aaaatccagt cttgaaatgc ctcggcttct tttttacggc ttttgaatgc
    12001 catgcggtaa atattttttt tcgttgatga tgtttacatc ttgtgttctg cccaatgaat
    12061 cgatgacctt actagtagta accccatctt taggcaaaga gtgaatggct ctagtaacct
    12121 gtttaagacc taacgcttcc gcaacatctt tcgccactgc ccaccattca tcattaatat
    12181 cgacaaatct tataatgtgt ccgttccaaa tttcagtttt cattcaacgt cctccagttc
    12241 aagccctagc aagctcgcta acaactcaac ttgctgatac aaaatttctt gatactcttc
    12301 aatagaaagt cgttctttaa actctgcttg tactaaatcg ttcaagccac gcaatactcc
    12361 gcttaccgtt ggttcttgtg gtagcaattt ttgatagtcc atttgttcgt acaaacattc
    12421 aaacgtttct tctagcgctt tgccttccaa gtttgctaat tctgccgctg tgtcttctgt
    12481 aagaattgtt gataatactt tttcgattgc tttattttgt gtcgattcca ttattatttc
    12541 ctcctattaa tccctttgcc cccacggctt gcgtttattt ttggtattct tccataaagc
    12601 gatcacattc atcttgtgaa attcttacga ctccatcaac tataatcaac tttaaaccgt
    12661 taggaacata tttctgcatc atagtattgt atgaaacgtt catatactta gcagcttgtg
    12721 ccttgttcat atattttgga aactggtact ttttttctaa taatgaaagt aacgttcctt
    12781 gagtttgctc tacttgttga acaactcgat cgtcaataat tttaaaagct ggatcatcaa
    12841 gcgtgagttt gtcaattttt aattccatat ttatcccccc tcgttagcat acatttctat
    12901 acaaagattt taaaaaaatg caagaggctc ttgaatacat ttctatacct cttataaact
    12961 caatatacca tcataccttt tcatttgcaa gaattttaac atactttttt gaacttttat
    13021 gtttcacaca tatttaaatc gtgatatagt agagaaaata tatgaatggt ggtgaaaatg
    13081 tggctagaaa aaaatacccc gaagcagtgg agacaggaca aagaattaga ctggctagaa
    13141 ttgaaaaggg actctcaatg gaacaacttg gaggtaaatt atccccaccg gccagtaaag
    13201 gtgccgttag taattgggaa aatggctata atcttcccaa taacaccagg cttaaacaac
    13261 tttcaaaggt attagatgta tccactacct atctattaaa cggatcttat acattaagag
    13321 atatgcatat gatgcccgaa attaagcgaa atgtgcttga aaagcaattg aaaaaaacta
    13381 ttgaaaaaaa taactctata attgattcta atctttctaa tgctatcaat aacatcgaag
    13441 aagataacct gactgtctca cagaagattt atttaacaaa cgcaataaaa tatcttaaaa
    13501 caggatctcg aattgaattt cctgacggga gtaccccaat tgcttatctc gcagctcttc
    13561 taaatacgct tttacgatat gactcattaa ttaatgattc agaaaaaaat acagatgaga
    13621 aaaaagagta tcaagaattt tatgaaaatg atcttctaaa agagttcaca gctcttgtta
    13681 ctgagctaaa taaacactat tcaaaataat aataaactgc ctcctccaac cgcccccacg
    13741 gcttgctaca ctatggaggg aaaactatta tggcaacatt caagcaatat gaaactaaaa
    13801 aaggtaaatt ttggctctat gaagcctatc ttggaatgaa caaaatgact ggcaaacctg
    13861 ataaggttcg tagacgaggt tacaaaacaa aaaaagaagc ccagcttgcc ttgtcacgct
    13921 tgcaagtgga ctacgataaa aacggtatta aaaaagcgaa caacgaaacg tttaaagaag
    13981 tttatgactt gtggctagaa acatataaaa ccactgtccg agaagccacc ttcatgaaaa
    14041 ctgaaatcaa gtttagaaag tggattcttc caaaatatgg aaatcttcgt gtgaatgaag
    14101 tcacggtaaa aaaagcccaa caaattgtaa atagctgggc tgaaactact gaccaataca
    14161 aggtacttca ttcaacttct aagcgtattt ttagctacgc tatcaatctt gggattattg
    14221 agactaatcc actagacaac gtattaatgc ctaaaagaac caccactaag cataaagaaa
    14281 aggtcaaagt ttacagtaaa gaacaactga tcaccttgtt tgattaccta aacagcaaac
    14341 ctgaaactta tcgaaacgac tttgataaaa cgttactacg ctttctcttt tatagcgggg
    14401 ttaggatcag tgaagccctt gcattgaatt ggtcagacat tgattttcaa gaaaatacgg
    14461 tcaccatcaa taaaacacta agccaatcca aaaatggcta taaaatcagt gatcctaaaa
    14521 ctgattgtag tgctggggtt cttccactag atgaaagaac aatttctata ttaaaaaaat
    14581 ggcagattaa tcagcggaaa tatatgctga cacttggtgt aactgatccg actatgattt
    14641 tttgtggtat ctataaagaa ctaatcacac accacgctat ttatgcaaga ttgattacta
    14701 tttgtgaaaa agctatggtt ccatttctcg gtgtgcatgt agcaagacac actcatgctt
    14761 caatgctctt ggacgctgga gctactatgg tagaagttca aactagatta cggcatacaa
    14821 aaattagtca gacgatcgat tgttacggtc acttggcaaa agaaacgaaa gaaaagactg
    14881 ttgaaaaatt agtacaacac ttaaatattg actagatggc atacaatatg gcatacagta
    14941 aaaaataaaa aaaggagaaa ccctatcata tcaaggtttc tcctagcttt atagtctatt
    15001 cttcttcttc cgccatgaat gtattgaata cttcttcaat catgtcccat tctgcgtctg
    15061 tttcgatttg ttctaactcg ccttctgttc catcttcgtt ttcgatgtat gcataagctt
    15121 gtaattctac atcttcatct tctgaaacac cagcaggata taaaagaaca tagttacgtc
    15181 caaattcttc ttgtccgtca actgttagta ggatttcata taatgtttca tttccttggt
    15241 catccaccaa tgtgatgtgt tcgtgtcctt catggtcgtg ggaatggtta tgttctgtca
    15301 taagattacc tcttttcttt tattattaaa ataattgctt acctgagccg tctaaataat
    15361 tttgtaagat catcacagct gctaatttat cgatgacttt ttttcgtttg gctcttgaag
    15421 tatttgcctg ctctactagc atacgttctg cctgaacagt ggttaatcgt tcatcttgat
    15481 aatcaactgg caaggagaat agttctttga tcttttctcc gtatgacatg gaagcttctg
    15541 cccttggtcc gatggaattg ttcatatttt taggtaatcc aactacaaac cggtctactt
    15601 catattgtgc tactaattcc ttcaaccgat caaagccaaa ctcttcttct gcttcattga
    15661 tccgaatgat ctctactcct tgagctgtcc agccaaaggg gtcactgata gcaacaccta
    15721 ctgtcttaga accgacatcc aagcccatta ctctcataaa ttcactccat gatttgagag
    15781 gtagtccttg acaattactt ccatgatctc atcgcgttcg tgacggcgaa tcaaatttcg
    15841 agcatcacga taacgaggaa tgtaggacgg gtctccagaa agcaagtagc cgacaatctg
    15901 attgattgga ttgtagcctt tttcttccaa agcccgataa acaatcgcta atgtttcgct
    15961 cacttctttt ttgcgactat cgtcaaaacc aaaacgtacg gtttcatctg taaaacccat
    16021 gattctacac ctctttcccg caaggtaatt ccagcttacc ttctacaacc ttattttact
    16081 cgaagaaagg gaaaactaca aacaaatatt ccaaacttcc gaataaatta agaaacacta
    16141 tttgtatgtt tttttccttt tttctgtagc tttatcaaaa gtactctttt cataaagtat
    16201 acgctatatt tctttgaatg tatgttttta cacaattttt tcataatatt cataaaaaat
    16261 tcgaatagct gctactaatt ttttgttttt gttgcttgtc ctgtctcata attgatcgtg
    16321 tagccatctt gaacattgta gataaagaca ttgaattcaa tttttttgtc ttcaatgctt
    16381 tctgcttcca attgcactcc cctggctacc agttcattcc cctcaaaatg aggagtgaca
    16441 cggtagcgta catgatgatt tgtttctttg atatatgccg caatatcatt ttcgtgagca
    16501 agcatgtatg gagcattcaa cgaacgtgtc cctgtcatca agtttttaat gttgttattt
    16561 tgaccagtca gctggaaacc aattaaatgg ctgcgattat ataaccattg cccgtcactt
    16621 aatttctttt gtttccaacc tgtcgggtca atatacaaag gctctctctt ttcagtaggg
    16681 aacaaatctt ttccaagcat agcattggct tgtcctactc tgttcaattt atcaatattt
    16741 gaatagcttt cccatgttcc tttggaaagg ctaagatcct cttttgtaaa ttccggctga
    16801 ttcccattga tttctatttc ttgatgtttt ccatcatatt ctggcacatc cgctacttga
    16861 gaagcttgtg gtgtggaatc aaaaatagtt gtaatctgct gaaagatatc tgaatctttt
    16921 tgtgtggtgg aaacaccgaa tgcccctgca atcacagcag cgacgatccc cacgatcgcc
    16981 ttggttgttt ttttgctcat ttctttactc ctttactaac agctccctaa atcttaacat
    17041 atgttcccct ataaataaat actagaaaga aatagaaaca gcaaaaaata aaacctgttt
    17101 taagatggct taaaaataca aaaaagagac ggattcctcg tctctaatca gtcaccactc
    17161 tatctgtaag gtatttatta ttattcaaaa attttcttca tactcaagac aatacactga
    17221 tttcacaacc gcttggtaaa cggtgcgcaa aagcttgcat ctgcggtaat aagtccatcc
    17281 aagttaaaaa cataagaagt tgtttttgct tggctgttct tattacttgt tgcagagcag
    17341 ctttttcaca acctatttta agcgggtgac gggaatcgaa cccgcgacat cagcttggga
    17401 agctgggatt ttaccactaa actacacccg caatacaaaa acagtatacg cctcttttat
    17461 tccttcctca agttttttta ttctttacgc ttctggtatg ttttgtagta aaataaaact
    17521 aatcaatcaa ttattggaga agatgctatg acgggaaaag aattagatct gttgctggac
    17581 acttgtttac tcgctgggaa aatcatgatg gaaagcaatg cggagatgta tcgggtagaa
    17641 gatacgatga gtcgaattgc tttagcttct ggaaatttcc gtttggtcag ctatgtcact
    17701 caaaccggtc tctttattgg tcttgatcgt acctcgacca tccgaatgga acaaattacc
    17761 aaccgttcga ttaatttaga aaaagtagta aaagtaaatg atctttcacg taaatatgtt
    17821 gcttcagagc taacattaga agaactttac tgcgaactgc aagcaatcga ccgagaccgt
    17881 tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag tgatcagtgg aacaattatg
    17941 gtgttatttg gcggaaagat gaccgattta cctgtgactt tagtcattgg aggattggga
    18001 tacgcagctt atctatatag tttgaaattt tttagaatca aatttttatc ggaattttta
    18061 tcttctttat tgattggcat agccgctatc tacagcgtac gttttggctt aggaagcaat
    18121 caggatctaa tcattatcgg atgtgtgatg cctttagttc ctggagttca aattacgaac
    18181 gctatccgtg atttgctagc tggacattat gtctcaggag tttctcgcgg aacagaagcg
    18241 atgatgactg ctacgatgat cggttttgcg attgctttta ttttccagct tttttattag
    18301 gaaaggaaca caagtatggg gacgatattt attcagtttt cttttagttt tttagcgact
    18361 gcggcatttg ccgttattac aaatgtacct aggcgttcat tgatttgttg tggtctttct
    18421 ggtgcttttg gctggatgat ctactggatc tgtgttcaaa taggcggatc tgctgctttt
    18481 ggttcattgc ttggttcttt aggagtagca ttcatcagcg atcttttctc taaacgtttg
    18541 aaaatgcccg taacgatttt caatattccc gggatggttc ctctagtccc tggtggttta
    18601 gcttatcaag cggttcgaaa tctcgttact gaatcctatc gagaagcggc tcattatgcg
    18661 gtccaagcaa ttatgattgc tggagcaatc gctttaggtc ttgtattatc agaagtcttt
    18721 aaccataaca tccgtaattt caaagtaaag cgtgacgcaa tcatgatgaa acgaaaaaaa
    18781 cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg tgaaagagac gttcagcctt
    18841 gagcgatgac aaaatatctc acaaacattg cgacaagtaa ccgcaggagc aataagaagt
    18901 aattatgagt attaaaaaaa gttttgtaaa aatagcttct cacattttta cataaaattg
    18961 acttaatact gagaaactga cttttgaaca ccctctatct gtaaaaaagg ggctgcgaca
    19021 tgatttttgt cacggcccct cttctttatt caaatttttt ccaaaacgtt cgttgataat
    19081 aaacgatcca tagagcggaa agtattagaa ctaaaccgat cacaggaata ttcactaacc
    19141 aaaatggata aaacaaagcc aacattccgt ataagacgat aagaataata ctgatgatca
    19201 atgaacccat catagttgcg actaaaccca aacttcctga acctctgtta aataattggt
    19261 taatattggt ccagttcagc agcagcaacc gataatctct agcgaaataa cgtagtgaca
    19321 ataagtagca gccgaataaa gaaccaaata gcaagctagg gataaaaaac aaaggcagct
    19381 gaaaagacag tccgcttaat aatgcgatcc caccagttaa aaaactctgc aatatccatc
    19441 cgaatctgaa cttttctttt aaatattggt tcatagagat tggcaaagaa cggataaaca
    19501 gaaaattttc ttgatccaaa gaaataagat tgctgataaa agaagtctga ttaactgtta
    19561 agaaagccaa tgcgatccct accaggaaaa caacaccgat taacttatta tctaaatcag
    19621 ttaattttat ttcgcctcct agggcaaaag caagaataaa aatgattgga gttaacaagg
    19681 aagaagaaag aacttgcata atcaaattag gttcttttac taattgagca ttgtaactaa
    19741 ataaaagctg atgcacattt tggttctttt tatgggttct ttttattttg aactcactgt
    19801 ttgaatcagt cagttgctct gtaagctttg gtactatcag ctgttttaca gcaaggaaaa
    19861 atcccagagc cgtgatagct agtattcccc aacccaataa accttgttga gaaagtggct
    19921 gactggcagc atcatagaat ggcaataata tcgaaatcgg atgccgatct gttaactcat
    19981 tttcaatagt ccccgtttga tgattcatcc acatgattcc agcaaatgcg atcagcatcg
    20041 agctgccaag caagatacta gaaactgttt ttctatgttt tttaaacact gctgttcgag
    20101 ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat aatcaaaaat agagaaaaca
    20161 aaaggattga gataatgata gtaagcaaaa tccctagatc agctcgccat ccggttaaaa
    20221 taaacatgac tagaagcgga aaaacaaaag gaatgatggt caaagcaact accaaaatct
    20281 ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa aaatacttgc aggtcatttc
    20341 cttcaaaaaa gatattataa atgactgata tactttgagt taaacctaac ataccaaaaa
    20401 gtgccacgta ataagtaaag aaacctggca atttaacaaa gttcatagca aacatagcaa
    20461 atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt ttgagacaaa agatagatag
    20521 ttaatcgctt tccctttttt cctttctctc ttgctttatt cgtgagctgt ggattaacat
    20581 ataaaagatt aactcgaatt aattctaata attgtcgctt atacatacgg tccatctcct
    20641 gtcgcttgcc ttccagccat tttcaaataa atcttttcta gcgtttcatc aggtgagcca
    20701 ttcaataatt cactaactgc cccctgataa atcaattcac ctttcttcaa aatagccagt
    20761 ttatcgcata actgttgtgc ggtatccaat acatgtgtag aaaaaatgac tgtttttcct
    20821 tttgcggcat gagccttcat catttctttc aaatcaaatg ctgcttgagg atcaagtcct
    20881 tgcaaaggtt catccaatac ccaaatatct ggatctggca gcaacgtgcc gatcaaaatc
    20941 gttttttgac gcattccatg tgaaaaactt gcgattgttt cattttgatg actatacata
    21001 tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt tttgtgtgtc taattcatag
    21061 gcagctgaaa tcaagtccca atattctcca gccgtcaatt gtaaaaaaat atctggggaa
    21121 tcaggcacat aaccgatttt ttgtttgata ctcaatcgat tttccgtcaa tttcattcca
    21181 tctactaaaa tcgttcccga tgatggttca ataatactaa ctaaactttt aatcgtagtt
    21241 gattttccgg caccattatg tcctaaaaat ccaaaaattt ccccttgtct tatcgagaga
    21301 gacaagtctt ttaatgcttg ttttttcccg taacttttac ttacattgat gaattcaatc
    21361 atttctgtac tccttttgtt tgttattact ttttattgta cccttcgtta ttggtggagt
    21421 aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg actaggctgc tacttgggtt
    21481 gttctcttct acaggaaaac actcttctaa caggtaagat atgagtcttt gttttcttta
    21541 aaatgattca ttttctatct tttcttgcat aaaattcttt agtttatcaa tacttctctt
    21601 tcccatctct agttgttctc catttgcaaa tgttactatt tcgtttactc tgctaataga
    21661 tgtaatgttt tcagtattaa tgatataagc ttttaaatct ttacataaat actcatattc
    21721 gagtgatttt ttatattttt taattgtgcc gtttacgaga atctcttcat ctttcaatac
    21781 taggcctatt ttatttttat aacctggcat gctcattaga taaaggattt catcgtaatg
    21841 gatatagtga tcgctattac cataacggac agttgtccac ctttttttcg tagcatgttg
    21901 aacaggatag ttttcttttt ctttcaataa gtaactttgg atgctttttt tatttttttc
    21961 ataagtggat ttgagtcaat atcttggaca cattttagta gagacggtta agccattttt
    22021 tcttttgcta attcttcaaa ttcctgtggg gttagatagt gaatcgagct atggattcga
    22081 tttcggttat aaaatccttc aatgtagcta aatagcgctc gatttgcttc ttcaaaatct
    22141 gagtagctag tcgtgtagac ttcttctttt ttcaatgagg catggaaaga ttcgattcca
    22201 gcattatcat aaggtgttcc ttttctacta taagaatgcc ttattttgtt ggttttaagc
    22261 cattgttcca cttctcttgc tgtgtattgg ctgcccaagt cagtgtgtag aatcattcct
    22321 tctggaatgt gataatgtat ttttgcttta ttcaatgttt gaatgacaca atccacagtc
    22381 attcgttttg aaaatgtata actaataatt tttttcgtgt gtaaatccat gattgaagat
    22441 aagtaacacc aaccattttt cttagtagga atgtacgtaa tgtcagctgc ccatttctca
    22501 cagatcgttt cagtagaaaa gtcctgattt aagagattct cttttgaaat gatcggttta
    22561 ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa ttgtttcatt
    22621 agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac ttgatggatc
    22681 tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt ctttttcaac
    22741 ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata atagacactt
    22801 ctagggattt ctaataaccg acataacaaa gaaacagtgt aatccttaca ccatttttca
    22861 ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct ttttttaaga
    22921 tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact tcttttccag
    22981 tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc gttgcttcag
    23041 ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc agttcaacaa
    23101 tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc gtagataagt
    23161 ttaatttatt cttgtctcta cgaaaagctg tc
//
LOCUS       DACJGB010000046        23039 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.046, whole genome shotgun sequence.
ACCESSION   DACJGB010000046 DACJGB010000000
VERSION     DACJGB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.046"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..2569)
                     /locus_tag="IYI34_001843"
     CDS             complement(<1..2569)
                     /locus_tag="IYI34_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559708.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSIPE
                     "
     gene            complement(2791..3675)
                     /locus_tag="IYI34_001844"
     CDS             complement(2791..3675)
                     /locus_tag="IYI34_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAR0559709.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(3770..4207)
                     /locus_tag="IYI34_001845"
     CDS             complement(3770..4207)
                     /locus_tag="IYI34_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559710.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            complement(4424..5335)
                     /locus_tag="IYI34_001846"
     CDS             complement(4424..5335)
                     /locus_tag="IYI34_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR0559711.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(5712..6350)
                     /locus_tag="IYI34_001847"
     CDS             complement(5712..6350)
                     /locus_tag="IYI34_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559712.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(6354..7124)
                     /locus_tag="IYI34_001848"
     CDS             complement(6354..7124)
                     /locus_tag="IYI34_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAR0559713.1"
                     /translation="MIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQE
                     ISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSTYWERNILSGEIYPPLK
                     IKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFS
                     FFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIER
                     EPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            complement(7542..7958)
                     /locus_tag="IYI34_001849"
     CDS             complement(7542..7958)
                     /locus_tag="IYI34_001849"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559714.1"
                     /translation="MMTREKEAYERFILLFVERFNQAFHTDYATNIFEDFENSYSYSV
                     ISSTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNGFSLHQVLWCLLGKKKI
                     IITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGI"
     gene            complement(8009..8390)
                     /locus_tag="IYI34_001850"
                     /pseudo
     CDS             complement(8009..8390)
                     /locus_tag="IYI34_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8516..9181
                     /locus_tag="IYI34_001851"
     CDS             8516..9181
                     /locus_tag="IYI34_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559715.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="IYI34_001852"
     CDS             complement(9578..11611)
                     /gene="brxL"
                     /locus_tag="IYI34_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HAR0559716.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="IYI34_001853"
     CDS             complement(11729..14251)
                     /gene="pglZ"
                     /locus_tag="IYI34_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HAR0559717.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="IYI34_001854"
     CDS             complement(14367..17999)
                     /gene="pglX"
                     /locus_tag="IYI34_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HAR0559718.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="IYI34_001855"
     CDS             complement(18063..21734)
                     /gene="brxC"
                     /locus_tag="IYI34_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HAR0559719.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(21759..22334)
                     /locus_tag="IYI34_001856"
     CDS             complement(21759..22334)
                     /locus_tag="IYI34_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HAR0559720.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(22334..22933)
                     /locus_tag="IYI34_001857"
     CDS             complement(22334..22933)
                     /locus_tag="IYI34_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HAR0559721.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         6993 a         4532 c         3344 g         8170 t
ORIGIN      
        1 tttctgggat agaaagaaca aatttacctg tatcatcaat agttgtttcg gacaaaacag
       61 cttgattttt tttcaatgaa accaaagctc ctgcttcgcc tgtacctgtg attgagcgag
      121 aagctgcatt gatcaatgtg gtacttaatt tagcaggctc aggaggaatc acatcgactg
      181 tcgttttggc agagtccagt tctgtcactt cttggccgcc tcttactttt ttagcagcaa
      241 ctatttgcac ttgatccccc gttgttagtc tttcttcgtt gggtacttga acctcaaaca
      301 aaccgccttg ttcgccttct ccccacggat tatatcctgt cccttcattt tcatctgttt
      361 gtccttgtgt gagagcagta atttctacag gtgatttcgt cgtatctgca taagtaatac
      421 gtaaagtcac ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg
      481 gaacaacgac atgcccatag atttttttat ctgcattcgt tggtactctt aaggattcaa
      541 tcatcggaag ctggttattg gcactgattc gattataatt tttcattccg gcacctgtct
      601 gagcaaattc atttgttaaa cctgcactag ggcttgatac aaaattacct aagtcggttc
      661 cattgatcga taagtctgtc atatcagaaa aataatccgg atctttttca aagttatcga
      721 acgttactcc tgcctttcgc cataaagcaa tttctgaatg agcgatcttc aaagaagaat
      781 cgccatttcc ttgaaatacc cagccaccta tgccattagt ctcgcttccg atccgtttgt
      841 tttggaaatc gaaataggta ggagattcta agtctacatt caacacatcc ccactgatga
      901 tgccagtctt atcttcccca gttttcccga ccatgactaa ataagttttt tcgccagcca
      961 taaagttcaa atcaatatct ccagttgtat ctacggtagc tccggaatct gcgcgtatat
     1021 cgatatttga tttttctcca gtaaccgtaa aatcagctga ccctggactt tctagaaaac
     1081 cagaagcgtc ataaaattgg atcgcttggt ttccttgatt gagtctgttc acataagatt
     1141 tgccatcccc tgcatttctt accttcaatg agcctccatt agttactgtt agtttttgcg
     1201 taccgttctc aaaacgaact gcgctagaac gaccactttg cttatcaata tcgatgactg
     1261 atccggctcc ttctacgatc aaagaaagtc ctttccctac aaaacgcatc ccattgtttc
     1321 caggttggtc gccatctcca gccattttga ccgagatttg tccaccactt gatacatgga
     1381 tttgactaga atctccttgc aggcgaaatc cgccagtagt cgctgtattg attgtgattt
     1441 tcccatcatt cgacacagcg actcttgatc ctgagcctct catttggata attccgcgat
     1501 tgttgtcgtt ggtaatattg atctttgtat ttaccgaaaa ttcacttcct gagccactga
     1561 ctttaaatgt gacattcccg ggtagcgtat cgacagcctc tccttcatcg ctgacgccat
     1621 ctgcccgatc atagtcaaga gagacctttg aaccactcaa gatatggagt cctggtgctg
     1681 tttttccact gcccgtctca tcacctgaat ctagaaaacc atagaatttt cctagatatg
     1741 gtccataaag ctgactacca ttgattacat ttagctgata gcttctacaa aaataagctt
     1801 tacccggagc ggcattcatc ccttctttca ctggttggac atccaataca ccggaaacac
     1861 catctaatgt gacagttgca cgaggggcgt aaatcaggcc taatttagaa ccatcatata
     1921 agtcaagtgt cccggtaaat tttatttctc ccgtaaaatg atatctgttt ccccaaaaat
     1981 cggacatata agcaatgata atggcatttc catcgatatt tggtatcaac ccacctggat
     2041 gagctgccgt tcctttaaaa ttctgaaact cgatcgactg aggtgtctca ttgatataga
     2101 tgtgatgtgt gccgaaatta tgagcaagtc cgccaaaatc gataacgata tctcgcttga
     2161 tctccaattc cctgtctaaa gtcaaaggct gagttaaacg aatcttagct actgattcgt
     2221 ctgcgagaag ctgcgtcagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa
     2281 caactctaac ctgtacctct tcactgctat cagacgtttg ttcagctact tctgaggtct
     2341 cacttgtctt attttcttct tgccttgtat tagaagtagg cgcctcactt tgggattctg
     2401 tagtttcaga agtaattgat tcttctgaaa aagtagtctt gtcaaaactt tgattctctg
     2461 attgcgttga ttcgcttgtt ttctctacat cggcaaaaac ggatgtcacc atcaagagag
     2521 ggaagacaaa caaaatcgac gcaatcagct tttttacata ttgattcatt ctccaccatc
     2581 ctctctgacg ttttgacaat tttattttaa cgttttaatt tatttgtaat tgtactttca
     2641 tatttaaaat atgtattttt atggcaaatt tttctgttag gtgaaaattt cttttttgta
     2701 caaaaaaagg ctgtgacaaa agtcttgtca cagtccctta ttccgaacaa ccgatgtcct
     2761 cctgacaact cttcggtcac aacctccatt ttaacaataa acctgtaaat gtgagggtaa
     2821 cactttgatt gtcacaggta atttatcccc ttcatctcca tcaacattcg tcgtcaattc
     2881 ttcctcatca gctaatgaca caactaattg ctcacaagta agataggcta gatttttcgt
     2941 agattcatct actcctttta ataggtctgg caatgtttta agcgtatcga tcaaagaaga
     3001 atcttttaaa taaaccaaat ggagcttacc atcatccact tttgcatttg gaagtaatgt
     3061 ttcaaaaccg cctactgagt tagttagtcc tatcagcaag atactgctct caatttcttc
     3121 cttctttcca tcaacttcta cgtgaaacga gtaggattgt gtggaagcta actgtttgat
     3181 tcctgacatg aaataagcta gttttccaaa tttcgtcttc ttctccgcat ctacatcatt
     3241 gattgcttca gggatagagc caattgcgac gacattcata aaatagtcgt catttatttt
     3301 tccaatatcc aatggcttga ctgattcaat cgaaaaatga ttgatcgctt cctcaggttc
     3361 tagcggtatc cctaatgctc gagccaagtc attcaccgta cccaatggga aaaatccgaa
     3421 atttggccga tgttcttgtt cagcgattcc actgattcct tcgttgactg ttccatctcc
     3481 gcccatgaca aacacactat gatacccttc cgtagccgct tcgcgagtaa agttttttgc
     3541 atcccctgct ttttttgtat gcaagacgac cacttcatca aacaccgatt ccaattttgc
     3601 tatcgctaat tgctcaaatt cctttgcttg ttcaccgcct gaactggggt ttaccactaa
     3661 taaaactttt ttcatcactt tctttccctt ctgattgatt tctttaatat aacgatagca
     3721 taaagaaaaa aattaggctg tgatgaagta ctgttcatga aacattcatt tattttttga
     3781 caagataaaa tacccatact atgaaactga ggaaaattaa aacaatgata taggaaataa
     3841 aagaaaatga taggcattgt atacctaata aagtaaaaat cttaggtggc agataaaaac
     3901 acaaaaatag caagagaatg taatagcgtt tcttccactt tttgttatca gaccttatag
     3961 cgatcagagg aataccaaat gcataactga aaaactcttg atgtactgtt aatatgtcac
     4021 gaccttgaat aataaatgca acaatccccg ctataattag tgtgcctaat accaaaacgg
     4081 aaaattgatg aatatatttt atatacaaat gatccattgt acttcctgcc tttttatttg
     4141 tgagaaaaac aaatccgtat acgtgaataa cttctagaaa taaaagaaat ttgcaaaacc
     4201 actccattta tctacttcta cgtgcatttt ttaatatcgt tttctttatt taagattata
     4261 cagaaaaata tctagggtgg ctggattcta agtaaaaaaa atttttaaaa taaaaagaac
     4321 taaatcaagt aataattaaa ctactgttga tttagttcta ataaaaagat ttagatgaac
     4381 agttttattg atttaaatag ttcatatttt ttatttatac cgcttattta ctacttgctg
     4441 tactagaatc tgaagtgaat gtactcaaaa cattttcaaa gtctttgtct ttgattttta
     4501 cattagcgtc tttcaatact tcgccaatta ctttattctg gaaggtactg tcacttaatt
     4561 ttgtgtctgt tgcaatttct ttcaattctt ttttgtattt atccatgtcg ttgcctttgt
     4621 tttggttttt caccatttta accacataat attccgtagt ataagttgaa gcattggtag
     4681 aagtgatcac atcagaaact tgaccatctt tcaacttaaa ggctgcttct ttgacttctg
     4741 ccggaaccgt tgtagtagtg gaatcaaatt tcacttttcc gccatcttct tttgtcgtag
     4801 tatcagttga cttgtctttg gctaatttag agaaatcgtc tcccttatca gcagctttct
     4861 tcacatcttt tgcatcatct tcacttgcta cttggataat ttgagcttcc acttctggat
     4921 ggaaagattc ccaagccgtt tttaaatctt catcggtcaa tttgatatgt ttctttagac
     4981 cttcttgaaa ggctagactt tgtttgatac tgtctttgaa tgtttgttct gtgtaacctg
     5041 cagatttcaa ttgtgaatca aatgaatcac ccaattgttt ctttgtttga tcatattggg
     5101 aatcgatttc tttggttgat acgtcatctc catatttatc ttcaaatact tgataaacga
     5161 tcaactgttg cacagcggaa tctgctcctt gcattttttt caagctatcc attacttctt
     5221 gttcgctgat tgtaccgcct ttataactaa cggctgtttt ttcaccggca ttcgtacagc
     5281 ctgacaataa gccagtgcct agtacgatgg caagtgctaa aaatcttttt ttcatttatc
     5341 aattctccta cttttcgttt tttgcgaatt gctaaaaaca aaggtgttca agatgaaaat
     5401 catcaagaac acctttacta atctgctcga tacgtcctga atagatataa ggctacacaa
     5461 tccagaaagt tcgctcagca gaataaatgt caagtagcac atcacacctt aatatactac
     5521 ttcttttgtt accgtttttc aagtctatct tgcgttccgt atttaacgta actaattctt
     5581 agaacaaatg ttatcgattt tgtttttctt cttccgctgt tgggatattc aaatcagaca
     5641 cttttatgtt cgattttcca gacatttttt tgttgttttt attagaaaaa gtttgttttt
     5701 tcatttgttt tctaaagcat gatcttcttg tttcctattt tggtcagata tggatcatgc
     5761 gtgaccacga taactaacgt accttgggaa gcctgttgcg ataagatttc aacgacttgc
     5821 tccttatttc gtttatctaa tgatccagtt ggttcatcgg ctaaaatgat tttgctattc
     5881 ttcaataaga ctctcgcaca ggcgattctt tgcttctcac caccagataa tgtgtagata
     5941 ggcgtactta gcttcttgtt tatatttact tttttcaatg cctcatactt tgctgttcgc
     6001 ttatctgaaa catttaaatt cgaaaacttc aatccaatat ctaaattaaa atcgattgtt
     6061 tcatattcaa tcaacccaaa atcttgaaaa agaaaagaga gggcatttct ttttatcaag
     6121 cgcttctctc ttccagttaa atgttttgtt tcttgattaa ataaataaaa ttgccctgaa
     6181 ctgaattgat cattcaatcc taatatattc aataaagtac ttttccctgc tccgctctct
     6241 ccaactacag tcacaaaatc cccagcatcc gcagtgaaac gatccaaatg aagaatcgtt
     6301 ttgtttcgaa tgattttttt cgcacctttt atcactatca acggctccaa ttgttaacct
     6361 ccattcatca gcaataactg acttttcttt tctgtagaca aaataaaaac aactgaaaga
     6421 ataaaatcaa tgaagctaaa gaaaaaaata ttaaaaagat atttaggctc tctttctata
     6481 agaaaaagga agattacact tgtcgttccc catttgatga ggttcatcat gaagaaatct
     6541 ttatacgttt caaagagcga ataaccactt gaacgaaaga tagcgatttt ccgacaattg
     6601 tagacaaaat atatgcataa catgacatag ctcaacaaga agaaagaaaa gaaagtgagt
     6661 aaaaatatct gtatcaagta atgaaatgat ctgcttaaca ctttgacagc tatctcttca
     6721 gtctgagtat acggaaatga taactgcaag ttatcacgta actgactttc ttcaatcagc
     6781 tcaaataacc tttctttcga caaagagcct atcttgatct ttaacggtgg atagatttct
     6841 ccgctgagaa tatttctttc ccaatacgta ctgtttttca atgtcagtat ttcaacaacc
     6901 ggataatgtt cgatccaggg gatagaagga ataaatgtat aaatgggttg attgtttttt
     6961 ataaatagaa atttcggttc tttttcactg atttcttgat aatattctgt tatttgcgcc
     7021 aggtcatttt ttaaatctgt atgtctatat cgttcaggga tcaatataac agggtgcgtt
     7081 tctttttcgg ttatttggat caaccttcca gagacatcaa tcaaaggaaa tttcttaaga
     7141 taattcagat ttatttttat ccctctgcca aactgttcat tttcttcatt caagtaatct
     7201 gatacattga ctaatatact tccgttttta ttcaaagttt catataattc ctgatcggct
     7261 gctgatgcaa aacgattgga ataaataaaa tccacgtggt ttttcccgat ggttactgga
     7321 taaaatacat gatagtcctc accagcgatt tcttttggtg tttcatggga aacagctaac
     7381 tgtgaagaag aaaaaacgat agttgctaat tcgacattgg caacggtcaa aagaagagaa
     7441 aacaacaatc caaatgtaaa aagaatgtat tggatatagt tgaacgcttc tcttaacgaa
     7501 gcgatttttt caactaataa aagagaaatc aaagagatac cctatattcc gctgaacaag
     7561 aaaacgtaca tctggaacaa ataaagaaat tgctccttat aatacataaa aataaaacca
     7621 ccaatgattc ctgcaattat gaatgtaata atgattttct ttttccccaa taaacaccac
     7681 aatacttgat gtaatgaaaa gccattattc aacaataaac tgatttcttt attcatcaat
     7741 aagaaaaagg atgataacaa aaggaataaa agaatcaatc caatagttaa atacactcga
     7801 taattatata cgtctgtact tgaaataacc gaatacgaat agctattttc aaagtcttca
     7861 aaaatattag tggcataatc agtgtgaaat gcttgattga atcgttccac aaatagtaaa
     7921 atgaaccgtt cataggcttc tttttctctc gtcatcaaga aaaattcacc ttcaaaatct
     7981 tttattttga tcgattcttc aatggacgtt acgcgaaagg aggtattaga atagggtacc
     8041 ttctcgtgag aagatttgac atccccttga taatacaatg tcatttcaag ctttggctgt
     8101 atcttgaagt tccaagatgt atctacttca ttggattcat ttactgtttt tttcatataa
     8161 gaaagctcag tttctgatga gacattttca aataccatga ttagctgctc atattcttct
     8221 tgcccaataa aatgttgtgg caatcgaatc ggagtataag tatctgtttg atggaaaaat
     8281 tcatttgtcc gatagagtac ctgtttattt ataataacca aggcaaaacc actcatgata
     8341 ccggtcacta tgattgtgag taaaactgtg aacttttttt tgattatcac cactctccct
     8401 tacttaaatg attctttgat aaaatcatac cgtttttcat caaaaaaacg tatctatcaa
     8461 atgtataggt catgtagaat aaatctatat actgtagaat cgaaaggaga agaagatgaa
     8521 catcggagaa aaaatacgaa cttaccgcag aaattgtaag cttacgcaaa aagaattagg
     8581 cgaaaaaatc ggtgtttccg ataaaacaat ctctagttgg gaaaattctc gtacaatgcc
     8641 agaccttgaa atgctgtcac ttttacatca gactttaggt ctgccgattg attttccttc
     8701 tattgaatca gctaccgaat caatagaacc aacattgccg gatgaacctc ttgttatcaa
     8761 gaaatcttat tcatcaaaac aacccttctc actcaaaaag acactatctt tctttctgct
     8821 cgtcttttgt ttggtctatc tttttcttat caattatcaa tataaaaatg aaacggttga
     8881 tcgattcaat ccttttttga aaccacaaat cggctatacc acactccctg taggatacgt
     8941 ggaaaattcc atcgaacatt gggtatgtaa tgatatcttt gttcatggta catggctgtc
     9001 ttttcacgct ggagaggtag aaaaaggtaa gaatcatgct gtaatcaaac acaaaggaaa
     9061 acacgtcatg aacgttcgcc tgctcacttg ggaagccatt ccctcagatt accaagaaat
     9121 cctcatccaa gactatgacg atgccttaaa aagaagccct caggagccag ataggcatta
     9181 agtaggagat gaacgtagag atagaaaaaa tcaatgcaca tgatcttatc aaaaaaaaac
     9241 aggggtacaa attaattgaa aaagctttga gatgaaagtt cctaaataaa aaagttcaga
     9301 atgataggta aaaaaatgac taactattct agtattaggc tatttgtatt attcttctgt
     9361 tatttttata tcaaaaaatc cagaaataac taaatatagg tatttctggc ttatagtata
     9421 atatttacgc cacagccccc aatgattctt tttggaatca tgatcaagga cggacataca
     9481 caaaaatatt ttgcccattc gatccgaata gattaacgac taatctagta agtaatatag
     9541 attggtacgt agtcaaacca gcaaatggaa aagttgtcta ttccacccca agggctttaa
     9601 agacagcatc ttccggcgtt tgataaaaaa tcaaattaaa ggtacccatt aattctgggg
     9661 gtacagttcc aatatcagca gcgcttatca taggtacaag tacttttttc gctccggcat
     9721 cttttgctac ttgtaaacag ttagctaaat tatctacctt aatcatagaa ccaccaattg
     9781 taatatcccc taaaataact aaacttggta atacaggttt atttaaggaa actgagcata
     9841 aagctattat agttgataac gataatgttt ttgtcatacc aatgccattc atatcttgaa
     9901 catgaacgac aaaatctttt gtcgtcaaac taatagactg actaacactt ccataattag
     9961 ctcttaaata tcggtatgcc gaatccatcg cttctcttgc tccagcatct gaattgatcc
    10021 cagtacgttc aaactttcca tttcccgaag tcatttgcgt ttccaattta taggtaccta
    10081 attgcccagt tttgccataa gataccgtgt acaaatgccc tggcttccct aacccttctg
    10141 gaatcaaagt tcctccacct tgttcaggaa cagaaacata ctcttctcgc tcttcttcta
    10201 aatcaatgta tgaaaaattg acatcataaa attccattcc accaattttc tttaattgtt
    10261 cttttacacg acgacgcatt tcaagagcaa atcttagtat ttcttcgaca tcactttttt
    10321 caaatctacc atcaggatac aacagtttca tcatcccaga tacaatccga cgaactgcaa
    10381 tcgtatcccg ctgatttaat tgagaaccaa gtttgaaata tttatcaaaa ctatcgccaa
    10441 aactaacttt tcgaagctca cgcatgactt cagaaaaata atctgtaatg aagccatagt
    10501 catttgtaaa aaaatctggt cgatatttag gaatttccca acctggtaaa taacaatgca
    10561 tccgatccaa aaaggctgaa tccgtcccca taacctccgg aaatggctca aataaattcg
    10621 aagttttcaa tattacatca acgctttgat taatatttcc tacaaaagcc attgatgctg
    10681 aagcattctt ttcttctttg cctctagcaa aagatcctga agccatgtaa tctttcatga
    10741 tttgaacgcc atcattatcc ttaaatttaa tcccagcaac ttcgtcaaaa gcaacacagt
    10801 cccacatacc tacaagtccc attgtacgac tagccatatt atagaaaagg tttgctactg
    10861 ttgtttgtcc acctgaaaca aggattgagt ttggtgaaat ttctttatat acatgcgatt
    10921 tacctgtacc acgtggtcct agttcacaga gattaaaatt attttcaatt aatggaataa
    10981 gtcttgctaa atgaagccat tttacccttt tattaaattg tgtaggttcc ataccaatgg
    11041 accgtagaag taagtcaatc cattcatttg tagtaaactc cttgcgagcc atcttgtaag
    11101 cctcaatgtc taaaccaggc atttggattg gatcaacctt ttttgcaata aaagggctat
    11161 tccaacgatc attctcatca taccaatatt ccatcgaaac aatacaccag atccccccac
    11221 ctaataatcg atcaaatttt ttcggatact cttcactaat ttgaacattt tttaatccca
    11281 ggttagagaa ttctgcttca taacaatctt tcttttcatt taaatgtaca gttactttat
    11341 caataactgt ataaccaccc aattcacgaa tttttgcttt tactttttct gcttcatcag
    11401 gacgcacata gtttcgagca agaatatttt taacattttc cacacctttt tcaaccaatg
    11461 tttcatccaa tgacgaagca tacataccaa gtaaatattc caatacataa acaggtacat
    11521 tcgcaccttc tttaattttc tttgttaaat cttttctgac aatctttccg ggaaagaatt
    11581 cagataactt taaaatttcc tgttgttcca ctagaattcc tccatgtgtt cagtcattat
    11641 aatagcaatt aaagtatatc atacttaacc agtaggcttg ataatacaaa agaaaaagta
    11701 tccacaaaaa attgtggata cctcgttttt atatatcaaa atcaaatgat tctgcaaaag
    11761 gattatctat aatgaatttt attcgctcgg tcaaatcatt tttttgattt tttatcacta
    11821 aataataagt atcttcaact gtatatcgac gatttacaaa ttcaaaaaca aatttagtaa
    11881 accgctgatt tgcatcttct gtttttgaat ccgcaacata gatttcttca tttgagatac
    11941 gttcaccata ttcatcttca aaataaagcc agaaagatgc ggggtcataa acatcactaa
    12001 ttgcattttt ttgatagaat tctaacgtgg ttgataatcc tgtaattctg cggttgctag
    12061 tcattaattc aattgctact ggttcctttg cattgctacc tttggctacc gtaatttcta
    12121 gaacagggac tagcatttct tgaatagatg accctccatg aacgtagctt tgtcccccgc
    12181 cagctttctt aaaaatactt gaagtaatcg ggacacaaat gtttctagtg tcatcatttc
    12241 ctaaagaatc tcccaaagag taatttttcg taccaattat ttcgtattct tcttcactaa
    12301 gaataaaacg acgttctaca cgatcttttt catttctaga tggattttca atcttatcta
    12361 cttcttcaac gtggcttctt ttataaataa atccatgatc agccgtaact ataaaacgat
    12421 agatactgcc atcattatgc aagcgattaa taagtcggga aatttcgctt atcgctaaat
    12481 cacaggcctc aaaaactcga ttttcattcg agtgatcacc tgttctatcc acttgatcgt
    12541 gataaatata aatgactgat ttgccattaa atacttctcg tacctcagcc ttattcatct
    12601 taaccacatc atcataattt aaagctacag catttgcatc tcgttgctgg agaatcgcat
    12661 ttcgattgga taaaccattc gtttttttcc catcaatcag tacttctgtt ccttcattgt
    12721 aagttagttg attgtttggt aataaggcgg ctttaccgaa ttcagtaact gaaggtaata
    12781 cagaaagaaa gctagccatg tttgttgaat acttcttttc tctaactaac ttacgttcta
    12841 aattcatccc taactcatat cgaaaagcat ctgaaataat aactacagtt ttaacagagc
    12901 gtttcattac agcttgatta taaaattttc gaatggatgg acgattctta aattcaaaag
    12961 tttgattcca aatttgtcct gattgatcaa gaaacttcga ataatttgct tcaactaatc
    13021 gatacaattg gccatatttt tgcttttctt ctatatgatc actattccaa ataaatttcc
    13081 gataatgcgt atcaacccaa tacatttcct tctcatattg agttagcaaa ttatccatgt
    13141 tcatcggcaa gcacaatctg tttttatcca caagataata agcatgtttc aataattgat
    13201 actcataaat ataatccgat gcgaaatgca ttttttcgcg ctgctttaaa ctatcaaaaa
    13261 ttgtttgtcc tgcaattttc gttgtagtgt cagaagcctt taatcgttct gcataaaaca
    13321 ttaatatttt tgcatgaatc tcagaaaata cgtctgcttc aattagatca tcaatttcta
    13381 tactgtctaa taatccatca ccatcaatag cctgatatac ttttttagat agctgttcaa
    13441 agctttccat gtaacgtgta tcattcataa actgatccat gaaagtaata atgtttgctt
    13501 tcaaatgtag tatttcaaat cctttgaact tcttcggcat ctcttgatca atttgataat
    13561 aaaaatagtt caaataaaga cacatcgtca gcttttccaa tgttggctgg ctatccgtaa
    13621 agccaaattt catttgaata aattcccaaa atttagctgt taactgatac ttatcaagtt
    13681 cccttaaagg attctcccta ctttctgtaa agctttttag aactacacgc agtaaatcat
    13741 ttaatcgaat acttttcgat ttcaacaaaa cagccattat cattaattca atcgtttctg
    13801 atttattaat tgtcatatgc gtactaaatt caccaaaact tctcatgcga tcctttgcat
    13861 taaagaaatt acgatactgt ttaactgtct gatgaaatct aagtggaata gccaattcat
    13921 tcatgataat cgctaagcgg tctgcactaa attcctctga ataattcaat acactcaaca
    13981 aatgattatc ttcatcttta tcatcccaac gaggaaatgg cgcatagatt aaatagttag
    14041 tttcgggatc ttcatattcc aataaacgct tagttttaaa ttgtgcgtcc ttttctaatc
    14101 gatagatttt tgcattttga atgaccaatg agtcaatttc ttcaagaaaa tcctgatttg
    14161 aatcaaacca aaaaatgatt ttcctacatc cataattagg aaaacgttga tttaattttt
    14221 cttcaatttg ttgcgtattt atatctgtca tcaatctcac ctaatcgttc tctttctata
    14281 ccccttatat tgacataatt tggataattt cgcacgcaat ttaagaataa aaaagaaagt
    14341 tgttagaaaa caactttctt agataactat ttttttggtt ctttgacccc ttttgcaaaa
    14401 atttggaatt tctcgccggt tttaggatca cgctgcaact tatcataatt gacaattaca
    14461 ccatcatcta agtctaattc aattcgtttt aatgctaagt gacccaactt ctcgtcatac
    14521 tcacgaactt cttttaattg cttctgaatc tttacaattt cttttgtcat tgcatttctt
    14581 tcttttgggt tatcacttgt tgaaatttgt tgctgacgca attcgattcg attgtcatac
    14641 gctttttgta gttcgtgtag gtattcagta cgtacttttc ctaattgatc tggtgtatag
    14701 cgatgcaagt acattaacgc tttaaagcca ttttgttttc ctgagtccaa ttgccaataa
    14761 attggtcgat tggagtatgt ttttaaatgg tctttgtaaa aatcttgtac gaagtaacga
    14821 cgaataattt ctcgacttgt ccctttgcca cctaaagtgt cagcgataaa ctggagattt
    14881 tcttcaaggg tttctttacc aaaagtaatc tcaacgaaat caatgaaacg attgacaatg
    14941 tcaatttggc tatccttgaa atactcatca tcggtgatga gtaaaacatt atcagtatca
    15001 ggtttaaatt tttgatagcg ggaaggatcc cattcgccgc cagcgaagat gagaccatct
    15061 tcgtctaggg agtaacgacc gaagatacaa cctattgaat aagaaataaa atccttaatt
    15121 tccctcgcct tatcaggtaa ccttgcagag atatattcat cttgaacctc ttttgttaat
    15181 tctttttcaa gagaataatg attaatgaat atgtcgttca gttcctcttc attcgatttt
    15241 agagtattaa attgattaac caactgccta cttagacagt taaatgcctc tgagagatct
    15301 tttttaaaca tataacttaa caaaatgttt cttctaaact cgtatgacgt ttcaagagca
    15361 ttatagtctt ttctagctat atcgatattt tcatccactt tttctcttat tttttttaat
    15421 tttctctcag atgtaacttt aagaaaaggt agttttccca ttggtccttc atggtaatcc
    15481 aaggtgggag aaataaaagt taaaaagtga ttaactaatt tagagtttac atagccaaat
    15541 atttcttcaa agtcagttat atcctttgag aacagaacag atcctttagt ttcaaaaatt
    15601 gcctgattct gcaatctaaa agatacttgt ccagatgaaa gtgtcgacca agtaattccc
    15661 tttttaaaat agtaattaag attttgtggt ctagatttta acttcccatt ttcattaaca
    15721 taatttttta tttcatctcc atcattctcc cagttaacca cgtattcatt gtttccatac
    15781 cattttctaa aacgaccacc tttagttgta ggaaaccatt tttttctaga ctttttagcg
    15841 tcctctaatg ttttagcatc aaactttatt ttgttaaatt ctacttcaaa ccaataccgc
    15901 aaaaatttat tgttatttcc ggttgccaac ccttgttttg catctgcaac atcgttagtt
    15961 aattttcctt gagcaaaatc agtgattacg ttatcgcttg cccagtaagc aatcggcatt
    16021 cctgggattt ttttgaagtt gtcttgatta gtactataaa aataatcgca ttcaccatgc
    16081 tgatgaattg cttctaacgt tttaattttt tgaacttcca ttccaccttt aaaatctgat
    16141 aattttatat agcttccatc ttgttcattt gaatttttta tcgtaaacgt acaaattgga
    16201 acggttgctt cttcaaaagc tgaatactcc atttgaatca aactagaaat gtttctttca
    16261 ttaattaaaa atgagcgtaa cttttcatag ctagaaatga acatccaaac aaatggcgtc
    16321 ataaaggaag catatccatt atcaatcagc atttggctat tcaatttaat aaacacagaa
    16381 aatagatctt ttttgacatc agaatagtta tcatttacat atttttttaa tttcttatcc
    16441 attttattaa gataaggcgg attagtgact accgctacat atttatcaac tagcatttca
    16501 gccacgtcaa gaatgtgcaa gtatttttct tgtatttcct tcatcccata aagaatatcc
    16561 atattcgtaa attcgttgaa ttttgatata tactctctta cttttcctaa gtattcttta
    16621 gttattgcct gattattttt atcgactaac ttaatgattg atccaagttc tttcccattt
    16681 tcaaattgat caaaaattaa ctctttgtat tcattccagc gttcactata cttatcattg
    16741 ttcaattcac taatacgctc ttgatactcg tctgtcactt taggagaatc aataaactct
    16801 cttaaattcg gcgatagact ttttttcaaa aaaagacgat cgtattgacg ccctttcatc
    16861 ataattgcaa aataagacaa ttgatacgct cttttatcga tttcaagacc atataaattg
    16921 tattcaacga ttgcacgtgc agcatcacgc tcagaatatc ctaacgtaag ataaatttcc
    16981 atcaatacat cgaaggcgta aactaaaata tgcccagacc ccattgcgtt atcgataatt
    17041 tttagttctt ctggttttaa attacttaca gatgttgctt gttcaaaagc atatttatct
    17101 ggaattaagt atttcaactt atttcttaat tcactattgg gataatgatc taaccaatac
    17161 tttcctaaac tattgtcaac catataacga acgacccaat ctgtggtaaa tagttgagtt
    17221 gctgccggaa tatcttgttt tttgacaatc cctttataaa tattcaccac ttgattatgt
    17281 ggctcactat tatagtactg atacaaccac ccaataatct ctacctgtcc accagcttga
    17341 acatagaaat catcctctgg aatacttaaa agatcagcga ttacaccatc ttttttaata
    17401 taagaagctg taaacaaaag ttctgtataa tcttctactt cttcaaatag ctcaggtaag
    17461 taatcattca attgattaca gatttttata aaaatcaatt gatacagctt gtccattgct
    17521 tctgctgaat tatctgtttt ccaagttctg acttgttgct gttcattact agataattct
    17581 tcaaagatat cggcatcata aatatctgta ataatatctg gctcaatttt cccacttatt
    17641 tcactagata atacacgata cgtgtcaggt aaataatcat ttacctccat aaaccgaatg
    17701 gcaatcaatc ggttaaacca cgtataagca acctcttcga ttaactcttg aaaagcttct
    17761 gaataagaag tgacagaagc acgttgtttt agttctgcaa tcaatttgtt tctttgcgct
    17821 atttcttttc cttgaattct tattcctgat ttttcgattt cttgtaaatt gctatcaatt
    17881 ttcttcacat cttcaatttt tgattcatca attcctaaac gttttgcttt agtcattact
    17941 tcatcaatta attttctacg ggcattgacc gcgaaattct ttatcgcttt cttatccatt
    18001 tttctttccc ttctatatgt aacctttgaa aagacaggca cctttttatg cctgtctttt
    18061 cactagaatt gaatcgtgat ttctgtattt tcttgaagtt gttccttcag tttttgatta
    18121 atttcagctg ttaactcatc aatatctgct gctgacttaa tagttttaac atgaacgtta
    18181 agtgttctca ttggtactgt tttctgttga atagtaggtt ttactatcgc tatttcttca
    18241 gcttttgctg cagagtctat ttcatctttt tcagtttcac gaatcaagtt tgctgcttca
    18301 atttgtttac gtgcttgttc ttcttgacgt ctgcggtttt cttcattatc gaaacgttga
    18361 ttaaagatca aatactgttc tgctacgttt aatgataaga aaacaagatc atctatagta
    18421 ttcgcttgtt caatttttgc tttcagttct tgaaattctt ggtcaactat tggtacaaat
    18481 tgatccttaa aatcttttcc ttctaataat ttaaatgttg cttgtttttc ttccgttaat
    18541 ttatttaata tttcctgact ttccaactct aacagttgat tatattgttc aataaattct
    18601 tgaatcaact catttagttt catcatatct gaataaggat cagccttttt caaaatggtt
    18661 ttcatctcat gaatcaattg atcaatttct tggctagaag tttgacgccg acttttttcg
    18721 attaatctaa ttttatcttg tgcattctcc cagatttttt tctgagtact attttgcttg
    18781 tggaaatttt taattttatc atatgtttca gcccagtcga ataattcatc gcgatatttt
    18841 ttgattgtat aaaaaaagtc ttcaacatct gacgtttgaa caattacttc aagatatttt
    18901 tttatttgct catattcata agcactaggg taggtataag tgttttgtaa atcaaaataa
    18961 gagcgtaaat tgtctaattg accttttaaa tatttcttga aattttccat tgtatgttca
    19021 ttgttatttt catcaacaat attacgactt tgaaacagtt ctctaccgat ttcgtcaacc
    19081 aatttttttg cttttggcga aatcaccgcg cttggttcaa tttttatctt ttcactttct
    19141 cgtcgttttt gaataatcat tacaatacta tttggatcat ccatccgtga aatttgtaca
    19201 ttgttataaa gtaaatcaat tttaccagac atgaaaagtt tagcgatgca ccattccaca
    19261 tcaagttctg tgaatccata cggttgtttt gtttcaaaac gatcacgaat tgttttcatc
    19321 gtaatcgtta ggtgatttct cttttgatca ttgataaaat tgaaaatttc ctgaactgct
    19381 tgctcatttg atggatcttc aataatttca tttttgcttt ctttcactaa atataagata
    19441 tcttcttttt ctttaggcgc atcaatttgg tttaatttat ggtatacttc ttcagccatt
    19501 ttctgaagtg ctgattggaa ttttgcttta aagtcggagc ctgtcagttg aatttctgca
    19561 ccgttataga aaaattttgc agatttcaac agttcattta attgatcgtt gatacttgca
    19621 cttagttcct gtaattcacc agatttttcc gaacgaatcg cgtcaaagtt ctttactaca
    19681 ctcgaataag tcgttgttaa ataaagattc attctagcat acattgctat atcatcttta
    19741 aattctttat ctgatggcaa ttctacgaat aataagttac gggaactctt aagcgcaagt
    19801 tctgcctctt gacctgctaa atcagagtat ggtgaaataa aattaactgt catttcagca
    19861 tttccagctt gcttgtatgg acgttgatct aacacttgtg cataagtaaa cgtataccgg
    19921 tttttaaatt tatttccacc tgacatgcct aatttttcat aggcaaaact atttcggtct
    19981 agaacgccgt caaaaattcg tttagatagt tctacgatca aatcactatc tgtaatattt
    20041 tgatttttaa tctcacgagt aatttcctgt tcttcatcag tttggaaaat ataactatct
    20101 aagttcttcc gcacaaagtt ttccttaatt aaaaggtcca atgcttcttc tactcgtttt
    20161 ttagtttcca ggcggtcttc gttgatattt cttatcatca agctcgtaat attttcaaca
    20221 tttggtttga tggcttgatc aacatattta atcatgaata aaattcgtaa gacatttacg
    20281 ttaaagttat ctatatcagt tggtctttga atatgagaat tatttgtagc ttgtgtaatc
    20341 actcggctaa ttgctgtatc aatccaacgt tctaacgagt aataaaactg atctaaagaa
    20401 accaaactgg ttgtatcatt ttccttattt cgttcagcgg cttctttaaa cattgctagt
    20461 aatgaacgtt caccagacga taagtttgat ccttgatagc tgtgattacg aatctcggtt
    20521 agaatttgcc ccaataaatt gaattgatag ggaataaatg gataaacttc tgcgtaattt
    20581 tcagcatctt ggaagagctt taattcagga ctatcaacaa agagtaattt atttttcaat
    20641 tctgtttgaa cagtttcaaa ttgtaaggct aaagaatcat tggcaaactc ttttttcttc
    20701 aaaatacgtt ctttaattac ttcatcggca tttgctgaag ataaactcac agttgtggca
    20761 aaacgatcct tgatcttaga aaaatcatta tcagaattgt tatcaaagtt tgttacgtga
    20821 tcaatcgctt cctgacttgt cactaatacc caagccttac cttcacaaat tcgacccaaa
    20881 tcttccacaa cggtttgtaa ctgcaaagtc aactcacgat taccgccgat atattgtccg
    20941 acctcatcga ctagaaaggc aatatgatgg tctgcttctt tggaatcaag atagctttta
    21001 actagatttg caaagtcatc aatgcttatt aaataatttc ctgatgcttt ttctaaaaaa
    21061 ttatcaatag cggcttcact cataaaatcg attttaagtg ccgcttcttt gatatcatct
    21121 tggatccaat caaactcatg gcgctcttct tgccattttg ccccattaat ctcttcaaaa
    21181 gcagctttga atgcatcaaa cttttcttct ttaattaacc gacgttcaaa atctgctaga
    21241 tgtggaatcg tgcttaatcc ttgcatttcg ttaaatacct ttaaaaaaac attcacgatt
    21301 ccgtttttgt cttttttacc acctgcatca ctttttgaat caatattaaa cagaataaca
    21361 tctgtattta tttgtgagca ccgttccatc gttgccaaag tcagtggatc tttgattttc
    21421 ccatcttctt tgaagtaatc aatggctttc ttgtcattta tttctcgatt tgcgagcaag
    21481 taagaaataa tttttaagaa atgagattta cctgaaccaa agaacccctg aatccaaaca
    21541 cccattttat ctgttggtgt attgatactc cgttggtagg catcaaaaat tgttttaaag
    21601 tgtttttgta gttctttcgt aaccacgtat tcttctaatt cttgttgaac attttcatcc
    21661 tttgcttgac cgaccttaat aactccttga atatttcggt caattggttt tgcaaaaata
    21721 tctttaatta gcatttaatc tcctaccttc catcccaact aaattctaaa tgctcgataa
    21781 taattatcat ctttattggt attaagaatt gtcaaactta attcatcata ttcccctgga
    21841 tacatcatca caactggtac gcgatcaaat acctgatgca ttttatttaa aatagagtgc
    21901 gctctaatta atggatataa ttcgccaatt cctgtcagga acacaactga tttttctggt
    21961 gtatgggcat ccacatattc aaaaaataca ttttcttcct caggtaaatc cattttcaat
    22021 aaacgaccaa tgctttccac taatttttgt aaaccacgtc gttcttccat cttaatcgct
    22081 ttttcagtga aaccgcgttt ttctagatac tctaacataa tcacatagag attataaact
    22141 ttcacttcat aattatcacc aacagatgtt ccagatatat cattaatata atctcttaca
    22201 attaactcgt ctttcggatc gtagtcaaag atataatagc ctacttcatt acctaaacca
    22261 cgattcaata aaaaatcttc tttttgaatt acttcattca acttttccaa atgctcttga
    22321 atcgataatg acattataaa attcctccca gtgctagtac aatatcttta cgattcttgt
    22381 caactaacca ttgaatcaat cgtttatcta ataatatcgg agtcattcgt ccatattctt
    22441 tttcccctct gactaatcct gtttcaatta gattcgtttg ataagcggca ataagccgtt
    22501 taatcgtata atctttccat tttgaaatat cttctgaact tctccgcttc tctgtaaaaa
    22561 aagaacggta atcaactggt gttaattgca aatttccttt acgtagattt tcagcatata
    22621 cctcagtcat aaattcatta aaaagatcat caatcataag gatggaaatc aaattcacta
    22681 acttttgatt atctatattt aactgattga aaagttgcaa tccagcttca tccagttgac
    22741 taactctcct tttcacgttc tgatagattt ctttttgacg gttactgctc actgcaaaaa
    22801 aaatattttc atgttcattt ttctctttaa tttctatatc tgtatatcct tctagtcgta
    22861 attgaatata taacttgaac tctgaaaacc aaaagcgttt agaaactaga ccagcagaat
    22921 attttttatt caatagattg cctcctaatc atctataatt caataagtaa acaagtgttt
    22981 tttgctcatc gttagcattc attttttagt atctactata caatacatat ttattatag
//
LOCUS       DACJGB010000047        22909 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.047, whole genome shotgun sequence.
ACCESSION   DACJGB010000047 DACJGB010000000
VERSION     DACJGB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.047"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(69..1490)
                     /locus_tag="IYI34_001858"
     CDS             complement(69..1490)
                     /locus_tag="IYI34_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0559722.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="IYI34_001859"
     CDS             complement(1733..3610)
                     /gene="tdc"
                     /locus_tag="IYI34_001859"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAR0559723.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(3901..5157)
                     /locus_tag="IYI34_001860"
     CDS             complement(3901..5157)
                     /locus_tag="IYI34_001860"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAR0559724.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            5737..7038
                     /locus_tag="IYI34_001861"
     CDS             5737..7038
                     /locus_tag="IYI34_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAR0559725.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(7107..7706)
                     /locus_tag="IYI34_001862"
     CDS             complement(7107..7706)
                     /locus_tag="IYI34_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0559726.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            7961..8842
                     /locus_tag="IYI34_001863"
     CDS             7961..8842
                     /locus_tag="IYI34_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0559727.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            8855..10465
                     /locus_tag="IYI34_001864"
     CDS             8855..10465
                     /locus_tag="IYI34_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAR0559728.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(10542..11201)
                     /locus_tag="IYI34_001865"
     CDS             complement(10542..11201)
                     /locus_tag="IYI34_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAR0559729.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(11484..12149)
                     /locus_tag="IYI34_001866"
     CDS             complement(11484..12149)
                     /locus_tag="IYI34_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559730.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="IYI34_001867"
     CDS             complement(12296..12901)
                     /gene="dhaL"
                     /locus_tag="IYI34_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAR0559731.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(12929..13912)
                     /gene="dhaK"
                     /locus_tag="IYI34_001868"
     CDS             complement(12929..13912)
                     /gene="dhaK"
                     /locus_tag="IYI34_001868"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAR0559732.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(13914..14309)
                     /gene="dhaM"
                     /locus_tag="IYI34_001869"
     CDS             complement(13914..14309)
                     /gene="dhaM"
                     /locus_tag="IYI34_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAR0559733.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            14463..14783
                     /locus_tag="IYI34_001870"
     CDS             14463..14783
                     /locus_tag="IYI34_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAR0559734.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(14895..15764)
                     /locus_tag="IYI34_001871"
     CDS             complement(14895..15764)
                     /locus_tag="IYI34_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR0559735.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(15860..17164)
                     /locus_tag="IYI34_001872"
     CDS             complement(15860..17164)
                     /locus_tag="IYI34_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAR0559736.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(17161..17670)
                     /locus_tag="IYI34_001873"
     CDS             complement(17161..17670)
                     /locus_tag="IYI34_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559737.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(17845..19980)
                     /locus_tag="IYI34_001874"
     CDS             complement(17845..19980)
                     /locus_tag="IYI34_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0559738.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(20063..21718)
                     /locus_tag="IYI34_001875"
     CDS             complement(20063..21718)
                     /locus_tag="IYI34_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR0559739.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(21720..22034)
                     /locus_tag="IYI34_001876"
     CDS             complement(21720..22034)
                     /locus_tag="IYI34_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAR0559740.1"
                     /translation="MNLLFKSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            22204..22464
                     /locus_tag="IYI34_001877"
     CDS             22204..22464
                     /locus_tag="IYI34_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAR0559741.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            22478..22792
                     /locus_tag="IYI34_001878"
     CDS             22478..22792
                     /locus_tag="IYI34_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAR0559742.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6464 a         4715 c         4118 g         7612 t
ORIGIN      
        1 aaaactattc acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac
       61 ttattttttt atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg
      121 aagtgattga tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat
      181 gcatcactca atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc
      241 agcatgcagc tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt
      301 tcgaattctg gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg
      361 atacaataaa taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc
      421 attacaccat aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt
      481 ctagatacgc cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca
      541 cgagctactt ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc
      601 acaccaataa ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt
      661 tgacagtcaa tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg
      721 acatttgcta caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata
      781 aatacgcctt ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc
      841 atttcgatcc caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt
      901 tccaagtcag ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt
      961 ccgactttga aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc
     1021 cctagccata caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc
     1081 gtgatgatcc agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt
     1141 tctacattac caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata
     1201 cctgggaaca tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt
     1261 gctgttttta cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg
     1321 gaaaccattg atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat
     1381 gtccaaccga ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc
     1441 agaccgataa aggtcccaag acccatttta cttacttcag atttactcac cgttgacact
     1501 ctccttgatt ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa
     1561 caattcagcg aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag
     1621 acaacctcaa aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac
     1681 atgcgtcaga aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg
     1741 cttcgcttgc caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt
     1801 tgattttttc agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc
     1861 tggcacgcaa tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt
     1921 ggttcacaaa ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag
     1981 ttaagaactc attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat
     2041 ggttcaattt attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta
     2101 ccatattgaa atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa
     2161 atgacaagtc attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta
     2221 atttaccata tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg
     2281 ctgttgcccc tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg
     2341 cgcctttttc aaatacatat gtcgcaaagt aagagataac gtctctcatg cggatatctt
     2401 tgatgacgat accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta
     2461 cagattctgc ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt
     2521 tgttttctgt aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga
     2581 agttgttgtc ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt
     2641 gtaagtagaa gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga
     2701 taccgtcgat tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg
     2761 ttttttcagc tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt
     2821 tgtggtcaac tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt
     2881 ttagccatga atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt
     2941 ttccgctacg tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca
     3001 gattcatgat ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta
     3061 gttcaggtgt cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa
     3121 gtccttctaa gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc
     3181 tcattaattt tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag
     3241 attcataggc aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg
     3301 gcatcaatgt ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg
     3361 aatgggtacg catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt
     3421 gttcaaaact agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt
     3481 agttttgacg ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt
     3541 ggccgttctc agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg
     3601 attcactcat agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac
     3661 acctgttctt ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg
     3721 tatcaattat ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca
     3781 aatgtaaact ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg
     3841 tttcaaaatt ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt
     3901 ctaatcaatt actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt
     3961 accatcgaat tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt
     4021 aatacttata gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt
     4081 tgtggaaaca agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat
     4141 tttaccgaat ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa
     4201 tgcttgcgtt attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac
     4261 ttcttctgcc aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc
     4321 taagtcttcg atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc
     4381 gcgatcatct tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc
     4441 aagccaaacg gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa
     4501 tgggatcgtt aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa
     4561 tccagctgtg atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt
     4621 tttatgcaga tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga
     4681 gattcctact tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa
     4741 gtttttaccg tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt
     4801 gttcacaaat gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc
     4861 gtccacgtta tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct
     4921 tgggtcgccg atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa
     4981 ttggaatctt ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt
     5041 tggatcaacg ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc
     5101 ttcgtttgtt tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt
     5161 actgacctcc gtgtagtttt tttatgaaag caggaacctg ctagcatgtg taattaatta
     5221 tttcttcagg ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa
     5281 atctcgcggt accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa
     5341 cgctgtcgcg tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt
     5401 actggtttgc agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg
     5461 atcttcgtct tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa
     5521 cagaaaaagg atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc
     5581 cgaacatttt attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa
     5641 tttatcgaga agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg
     5701 acaagtaaaa aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt
     5761 ttaggagcca cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc
     5821 cgatcatcta caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa
     5881 gaaccgacag atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac
     5941 gattatgacc aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca
     6001 caacagcttc ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct
     6061 actcagatct ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac
     6121 tctcaacagg cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg
     6181 atcgaacaac tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa
     6241 gacgaacaag gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg
     6301 gttctttcac aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc
     6361 cgattggtcg tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa
     6421 gaaatcggtg actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat
     6481 ttttcttatc gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt
     6541 tatcgaacat caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa
     6601 gctaaaaaag tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg
     6661 ggtcacttga atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg
     6721 cagcaagaag ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca
     6781 gacatcagcg actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt
     6841 caaggtttcg atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt
     6901 acggatttct atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta
     6961 gacggaaaaa atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa
     7021 gcacgcgaat ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt
     7081 atcgtcagtt tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg
     7141 acttctgtcc attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt
     7201 ccttctacca ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact
     7261 tttccttctt tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta
     7321 tacgcatcca tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa
     7381 tcgatataaa attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca
     7441 atcagtgttt cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa
     7501 tcattcataa atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta
     7561 aaaatcgtcg ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg
     7621 gttcctatgt ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct
     7681 tgtgctctct tttcaaaacc gttcatttgt agagcctccc gtaattttaa acttttataa
     7741 tatatatagt tcaaatttat tctgaggttt attataacta tctattgaga aagtatcaag
     7801 ctagcaattc cttgtttatg aattcattga cagaaattta taaaaagaat attatgtact
     7861 aaaataataa aaaaaagttt tataaaaagg tataaaacct ttttattaaa ctatttaatc
     7921 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
     7981 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
     8041 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
     8101 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
     8161 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
     8221 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
     8281 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
     8341 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
     8401 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
     8461 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
     8521 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
     8581 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
     8641 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
     8701 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
     8761 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
     8821 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
     8881 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     8941 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     9001 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     9061 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     9121 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     9181 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     9241 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     9301 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     9361 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     9421 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     9481 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     9541 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     9601 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     9661 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     9721 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     9781 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     9841 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     9901 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     9961 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
    10021 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
    10081 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
    10141 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
    10201 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
    10261 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
    10321 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
    10381 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
    10441 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
    10501 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
    10561 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
    10621 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
    10681 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
    10741 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
    10801 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
    10861 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
    10921 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
    10981 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
    11041 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
    11101 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
    11161 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
    11221 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
    11281 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
    11341 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
    11401 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
    11461 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
    11521 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
    11581 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
    11641 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
    11701 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
    11761 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
    11821 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
    11881 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
    11941 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
    12001 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
    12061 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
    12121 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
    12181 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
    12241 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
    12301 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
    12361 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
    12421 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
    12481 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
    12541 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
    12601 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
    12661 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
    12721 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
    12781 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
    12841 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
    12901 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
    12961 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
    13021 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
    13081 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
    13141 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
    13201 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
    13261 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
    13321 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
    13381 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
    13441 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
    13501 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
    13561 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
    13621 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
    13681 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
    13741 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
    13801 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
    13861 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
    13921 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
    13981 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
    14041 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
    14101 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
    14161 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
    14221 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
    14281 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
    14341 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
    14401 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
    14461 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
    14521 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
    14581 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
    14641 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
    14701 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
    14761 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
    14821 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
    14881 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
    14941 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
    15001 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
    15061 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
    15121 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
    15181 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
    15241 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
    15301 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
    15361 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
    15421 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
    15481 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
    15541 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
    15601 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
    15661 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
    15721 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
    15781 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
    15841 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
    15901 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca
    15961 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
    16021 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
    16081 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
    16141 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
    16201 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
    16261 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
    16321 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
    16381 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
    16441 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
    16501 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
    16561 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
    16621 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
    16681 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
    16741 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
    16801 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
    16861 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
    16921 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
    16981 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
    17041 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
    17101 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
    17161 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
    17221 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
    17281 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
    17341 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
    17401 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
    17461 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
    17521 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
    17581 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
    17641 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
    17701 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
    17761 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
    17821 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
    17881 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    17941 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    18001 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    18061 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    18121 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    18181 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    18241 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    18301 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    18361 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    18421 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    18481 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    18541 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    18601 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    18661 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    18721 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    18781 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    18841 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    18901 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    18961 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    19021 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    19081 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    19141 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    19201 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    19261 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    19321 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    19381 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    19441 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    19501 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    19561 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    19621 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    19681 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    19741 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    19801 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    19861 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    19921 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    19981 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    20041 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    20101 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    20161 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    20221 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    20281 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    20341 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    20401 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    20461 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    20521 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    20581 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    20641 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    20701 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    20761 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    20821 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    20881 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    20941 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    21001 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    21061 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    21121 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    21181 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    21241 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    21301 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    21361 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    21421 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    21481 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    21541 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    21601 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    21661 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    21721 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    21781 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    21841 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    21901 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    21961 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattta
    22021 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    22081 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    22141 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    22201 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    22261 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    22321 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    22381 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    22441 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    22501 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    22561 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    22621 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    22681 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    22741 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    22801 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    22861 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagt
//
LOCUS       DACJGB010000048        22771 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.048, whole genome shotgun sequence.
ACCESSION   DACJGB010000048 DACJGB010000000
VERSION     DACJGB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.048"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            3..371
                     /locus_tag="IYI34_001879"
     CDS             3..371
                     /locus_tag="IYI34_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAR0559743.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            551..1492
                     /locus_tag="IYI34_001880"
     CDS             551..1492
                     /locus_tag="IYI34_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAR0559744.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1561..2292
                     /locus_tag="IYI34_001881"
     CDS             1561..2292
                     /locus_tag="IYI34_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAR0559745.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2436..4616
                     /locus_tag="IYI34_001882"
     CDS             2436..4616
                     /locus_tag="IYI34_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAR0559746.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4636..5118
                     /locus_tag="IYI34_001883"
     CDS             4636..5118
                     /locus_tag="IYI34_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAR0559747.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5134..5895
                     /locus_tag="IYI34_001884"
     CDS             5134..5895
                     /locus_tag="IYI34_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAR0559748.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6026..7360
                     /locus_tag="IYI34_001885"
     CDS             6026..7360
                     /locus_tag="IYI34_001885"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAR0559749.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7667..8152
                     /locus_tag="IYI34_001886"
     CDS             7667..8152
                     /locus_tag="IYI34_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAR0559750.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8145..8828
                     /locus_tag="IYI34_001887"
     CDS             8145..8828
                     /locus_tag="IYI34_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAR0559751.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8954..11599
                     /gene="polA"
                     /locus_tag="IYI34_001888"
     CDS             8954..11599
                     /gene="polA"
                     /locus_tag="IYI34_001888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAR0559752.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11644..12480
                     /gene="mutM"
                     /locus_tag="IYI34_001889"
     CDS             11644..12480
                     /gene="mutM"
                     /locus_tag="IYI34_001889"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAR0559753.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12444..13073
                     /locus_tag="IYI34_001890"
     CDS             12444..13073
                     /locus_tag="IYI34_001890"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAR0559754.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13125..13457
                     /locus_tag="IYI34_001891"
     CDS             13125..13457
                     /locus_tag="IYI34_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAR0559755.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13769..14260
                     /gene="nrdR"
                     /locus_tag="IYI34_001892"
     CDS             13769..14260
                     /gene="nrdR"
                     /locus_tag="IYI34_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAR0559756.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14283..15656
                     /locus_tag="IYI34_001893"
     CDS             14283..15656
                     /locus_tag="IYI34_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAR0559757.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15656..16582
                     /gene="dnaI"
                     /locus_tag="IYI34_001894"
     CDS             15656..16582
                     /gene="dnaI"
                     /locus_tag="IYI34_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAR0559758.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16711..17151
                     /locus_tag="IYI34_001895"
     CDS             16711..17151
                     /locus_tag="IYI34_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAR0559759.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17295..17996
                     /locus_tag="IYI34_001896"
     CDS             17295..17996
                     /locus_tag="IYI34_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAR0559760.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17993..19114
                     /locus_tag="IYI34_001897"
     CDS             17993..19114
                     /locus_tag="IYI34_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAR0559761.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19129..20361
                     /gene="pepT"
                     /locus_tag="IYI34_001898"
     CDS             19129..20361
                     /gene="pepT"
                     /locus_tag="IYI34_001898"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAR0559762.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20493..21401
                     /locus_tag="IYI34_001899"
     CDS             20493..21401
                     /locus_tag="IYI34_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAR0559763.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21436..21708
                     /locus_tag="IYI34_001900"
     CDS             21436..21708
                     /locus_tag="IYI34_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAR0559764.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21719..22765
                     /locus_tag="IYI34_001901"
     CDS             21719..22765
                     /locus_tag="IYI34_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAR0559765.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7674 a         3974 c         5005 g         6118 t
ORIGIN      
        1 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
       61 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      121 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      181 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      241 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      301 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      361 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      421 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      481 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      541 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      601 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
      661 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
      721 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
      781 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
      841 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
      901 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
      961 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1021 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1081 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1141 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1201 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1261 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1321 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1381 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1441 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1501 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1561 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1621 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     1681 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     1741 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     1801 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     1861 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     1921 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     1981 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2041 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2101 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2161 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2221 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2281 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2341 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2401 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2461 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2521 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2581 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     2641 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     2701 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     2761 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     2821 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     2881 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     2941 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3001 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3061 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3121 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3181 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3241 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3301 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3361 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3421 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3481 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3541 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3601 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     3661 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     3721 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     3781 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     3841 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     3901 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     3961 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4021 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4081 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4141 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4201 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4261 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4321 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4381 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4441 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4501 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4561 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4621 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     4681 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     4741 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     4801 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     4861 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     4921 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     4981 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5041 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5101 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5161 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5221 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5281 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5341 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5401 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5461 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5521 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5581 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     5641 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     5701 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     5761 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     5821 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     5881 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     5941 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6001 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6061 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6121 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6181 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6241 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6301 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6361 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6421 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6481 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6541 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6601 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     6661 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     6721 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     6781 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     6841 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     6901 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     6961 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7021 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7081 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7141 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7201 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7261 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7321 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7381 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7441 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7501 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7561 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7621 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     7681 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     7741 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     7801 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     7861 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     7921 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     7981 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8041 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8101 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8161 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8221 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8281 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8341 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8401 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8461 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8521 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8581 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     8641 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     8701 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     8761 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     8821 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     8881 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     8941 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9001 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9061 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9121 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9181 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9241 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9301 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9361 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9421 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9481 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9541 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9601 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
     9661 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
     9721 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
     9781 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
     9841 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
     9901 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
     9961 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10021 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10081 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10141 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10201 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10261 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10321 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10381 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10441 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10501 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10561 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10621 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    10681 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    10741 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    10801 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    10861 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    10921 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    10981 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11041 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11101 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11161 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11221 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11281 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11341 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11401 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11461 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11521 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11581 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    11641 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    11701 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    11761 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    11821 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    11881 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    11941 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12001 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12061 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12121 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12181 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12241 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12301 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12361 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12421 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12481 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12541 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12601 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    12661 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    12721 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    12781 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    12841 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    12901 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    12961 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13021 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13081 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13141 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13201 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13261 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13321 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13381 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13441 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13501 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13561 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13621 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    13681 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    13741 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    13801 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    13861 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    13921 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    13981 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14041 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14101 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14161 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14221 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14281 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14341 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14401 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14461 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14521 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14581 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    14641 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    14701 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    14761 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    14821 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    14881 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    14941 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15001 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15061 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15121 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15181 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15241 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15301 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15361 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15421 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15481 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15541 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15601 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    15661 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    15721 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    15781 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    15841 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    15901 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    15961 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16021 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16081 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16141 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16201 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16261 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16321 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16381 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16441 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16501 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16561 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16621 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    16681 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    16741 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    16801 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    16861 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    16921 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    16981 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17041 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17101 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17161 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17221 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17281 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17341 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17401 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17461 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17521 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17581 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    17641 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    17701 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    17761 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    17821 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    17881 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    17941 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18001 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18061 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18121 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18181 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18241 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18301 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18361 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18421 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18481 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18541 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18601 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    18661 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    18721 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    18781 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    18841 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    18901 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    18961 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19021 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19081 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19141 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19201 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19261 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19321 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19381 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19441 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19501 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19561 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19621 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    19681 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    19741 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    19801 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    19861 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    19921 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    19981 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20041 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20101 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20161 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20221 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20281 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20341 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20401 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20461 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20521 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20581 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    20641 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    20701 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    20761 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    20821 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    20881 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    20941 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21001 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21061 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21121 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21181 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21241 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21301 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21361 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21421 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21481 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21541 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21601 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    21661 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    21721 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    21781 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    21841 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    21901 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    21961 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22021 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22081 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22141 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22201 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22261 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22321 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22381 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22441 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22501 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22561 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22621 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    22681 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    22741 agagaataaa gaaaatcaag ggtaggtctt t
//
LOCUS       DACJGB010000049        22608 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.049, whole genome shotgun sequence.
ACCESSION   DACJGB010000049 DACJGB010000000
VERSION     DACJGB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.049"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            89..274
                     /locus_tag="IYI34_001902"
     CDS             89..274
                     /locus_tag="IYI34_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAR0559766.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            341..736
                     /locus_tag="IYI34_001903"
     CDS             341..736
                     /locus_tag="IYI34_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAR0559767.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            726..1103
                     /locus_tag="IYI34_001904"
     CDS             726..1103
                     /locus_tag="IYI34_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559768.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            1104..1499
                     /locus_tag="IYI34_001905"
     CDS             1104..1499
                     /locus_tag="IYI34_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559769.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
     gene            complement(1806..2204)
                     /locus_tag="IYI34_001906"
     CDS             complement(1806..2204)
                     /locus_tag="IYI34_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAR0559770.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2396..3163)
                     /locus_tag="IYI34_001907"
     CDS             complement(2396..3163)
                     /locus_tag="IYI34_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAR0559771.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(3198..4421)
                     /locus_tag="IYI34_001908"
     CDS             complement(3198..4421)
                     /locus_tag="IYI34_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAR0559772.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLENHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            5402..6205
                     /locus_tag="IYI34_001909"
     CDS             5402..6205
                     /locus_tag="IYI34_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAR0559773.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            6790..7245
                     /locus_tag="IYI34_001910"
     CDS             6790..7245
                     /locus_tag="IYI34_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559774.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            7364..8422
                     /locus_tag="IYI34_001911"
     CDS             7364..8422
                     /locus_tag="IYI34_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAR0559775.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            8464..10413
                     /locus_tag="IYI34_001912"
     CDS             8464..10413
                     /locus_tag="IYI34_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAR0559776.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            10416..14057
                     /locus_tag="IYI34_001913"
     CDS             10416..14057
                     /locus_tag="IYI34_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559777.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            14457..14795
                     /locus_tag="IYI34_001914"
     CDS             14457..14795
                     /locus_tag="IYI34_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559778.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            14905..15126
                     /locus_tag="IYI34_001915"
     CDS             14905..15126
                     /locus_tag="IYI34_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559779.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            15146..17302
                     /locus_tag="IYI34_001916"
     CDS             15146..17302
                     /locus_tag="IYI34_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559780.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            17314..17547
                     /locus_tag="IYI34_001917"
     CDS             17314..17547
                     /locus_tag="IYI34_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR0559781.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            17537..18403
                     /locus_tag="IYI34_001918"
     CDS             17537..18403
                     /locus_tag="IYI34_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559782.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            18418..20388
                     /locus_tag="IYI34_001919"
     CDS             18418..20388
                     /locus_tag="IYI34_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559783.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            20555..20782
                     /locus_tag="IYI34_001920"
     CDS             20555..20782
                     /locus_tag="IYI34_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559784.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            20779..21273
                     /locus_tag="IYI34_001921"
     CDS             20779..21273
                     /locus_tag="IYI34_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559785.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFVL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            21448..21783
                     /locus_tag="IYI34_001922"
     CDS             21448..21783
                     /locus_tag="IYI34_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559786.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(22040..22429)
                     /locus_tag="IYI34_001923"
                     /pseudo
     CDS             complement(22040..22429)
                     /locus_tag="IYI34_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22484..>22608)
                     /locus_tag="IYI34_001924"
     CDS             complement(22484..>22608)
                     /locus_tag="IYI34_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR0559787.1"
                     /translation="EESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         8176 a         3288 c         4256 g         6888 t
ORIGIN      
        1 actagcacca cgggttaaaa catcctcttt ttctattgat tccatacaca acaatgtgta
       61 taatagattt tgtaaaggga gggtagctat gcctaagaca gcaagagaaa ttgtaaaaat
      121 tctcaaatct aacggcttca aaaagactgg acaaactggc agtcatgcaa aattttataa
      181 ttccaagact aaaagaacgg tcgttgttcc gatccacaat ggtgatgtac cactaggaac
      241 agaagctaat atctggaagc aagcagggct ttaacagcct tgccttccta tttggtatta
      301 ggcatactat cctctcttac actagaaaag gagcgaggaa atgaagacaa aaaatatttt
      361 ttatccggca atcttcgaga aagaaggaga agcttataac atcgttttcc ctgacttacc
      421 ggaaatatct acctttggaa atagtcttga agaagcatat ttaaacgcta aggatgcttt
      481 aggattagcg ctatatagtg taccagatgc agaatttcct aaaccatcag cagtggaaga
      541 attaactttg aaagccaacc aagtggtagt aatcatccaa ctaaacatta aacttttccg
      601 aagaagctta aatacaaaaa caattagaaa aaatgttagt gttccggaat ggttagttct
      661 tttagggaag gagaaaaaca taaacttctc tcaactactt caaaaagcac tagaagaaga
      721 gttgcttgaa aagtaaaaag gagaccgaac ctatgaaagc tacacactta aactatttct
      781 ctcgaacgtt aatcaataat ctattaaaag atacaactgg cattagcgtt agtaccaaac
      841 actttgccaa tgatctaatt atttttgagg ataataactt gcgaattgag atgtttattg
      901 aacaggcaac ccctaactca atacctgaat tctgctatac gcacactgaa aaaatgaaac
      961 atgcaaaatc agttaaagat gttgaagcat taagtgaatc actaacgaac gctttaaacc
     1021 tcgttaagaa ggcaaaattg ttatttgaat ttcaaaccga ctaccacaat gagttgcttg
     1081 ctcaatctga aattttagat taattgaaac aggagaagag aaagatgaaa caaacacaaa
     1141 cagaaaccgt taacgatacg cttgctgatc aattagcagg ttattataaa ttatttgaga
     1201 tgggtaaagc acagcgcttg atccactatc tttggaaata caaaattttg tttctttttt
     1261 gggaatcaac actaacattc tcagttcttt tttataagtc agatttttat aatcaacact
     1321 tagttttagg gagttttttg cttatctaca ccattttgat gcttaattta tatacctaca
     1381 tcacacttga agaacactta gtaattagcg atgcagggat cgagagaaac atcaaaaggg
     1441 tatccttcaa gatcacgtta aacaagccta ccgataaaaa gctctcagaa gcgttctgag
     1501 agcttttgac attttcttgt ataattttat cctaacacat tacaaagcac ctaattcgag
     1561 cttatatcaa gcaaatttgc tatttattac tcaatacata tctaaaaatt acttgatgga
     1621 aaattagata catggtaagc atgaaaagaa aatcaatttg agcagatgca gtacaataca
     1681 tagcaattta aaagctctca gaaggcttct gagagctttt aatatattct ttgataattt
     1741 gtctttctta aatccatacg tcctaaaaca agctgttttt gcttaatatg atcatctgag
     1801 agacatcaaa tcaacttttc tctcaacaaa tcattcaaag ctttcgacag attaatattc
     1861 tcttcagccg ctaaaacatt taaatagtgt tccacagtca ctgattttcg atcataattt
     1921 tgcttgtttc gagctttagc aactttcgta ttagctttta cgacaaataa attaacattc
     1981 tcagatcgct tgattcttgc atttaactct tgaacaggcg taggcgatgg aatttctttt
     2041 tcttgcaact ctaaaagcac caagtatcct tccaatactt cttgcgccat ctcaaaagca
     2101 tcttctaaag tttcaccatg tgtatataag tctggaaaat ctgggaattt aactgcatat
     2161 tcatttcctt tccattcgaa tactgcacaa aaaaagtatt tcatcgcatt gcacctctct
     2221 aattaaaaaa taatttttgc ttttttgttt caaaaaaaag aatcgcctac caaaaaaatt
     2281 gataggcgat cacaagctaa ttaacctcta aaggagatta ataccttgct cccaaggcac
     2341 tatatatctt agcatacgtc tgaagcatct tcaaacgagt aagatatatt ttgcattaac
     2401 ctaatttttt ttctctataa gttttttcat caaactcact agaaatatta aactggacag
     2461 caaaattgta atttctgcct tttctaactt tagcaaaatc aatattcagc attgattttt
     2521 cattaatttc ctcaactgat tcccttaaaa cacgtctatc aaaattcgat attgttttat
     2581 actcattttc tgttttcgta atctcacgaa ttttaactat ttcaatcagg ggattcttat
     2641 atatttcagc tttctcaaaa tctcctttat ttttggcgat aagatatttt ttataataca
     2701 tcgccaaata tttgtataac acgatgctat attttttttg aaaagatgct aagtcttcaa
     2761 aaacaaagaa cggatacttc tttttgattg agacaattaa gggaattaca tcttctgaaa
     2821 aactgaaaga tacactatgc tcttctaaat cccaatgaat ccgatcaata gcagatacag
     2881 tagtatacgt cttatatttt ttgtctagat tcgaaatttc ataaactgct tgtctttgca
     2941 aagtgttaac tatggttttt agttccgttt caagattttg atcaacatcc atgtaattaa
     3001 taatcgcttt aacaggtaaa tctactacgc ctgttttctt tagctttgtg ggtgagatat
     3061 aactgaacag caattgataa agttcatacg actttaaatc cattttcatg tcaattgttg
     3121 ctaagaactc ctcattgtac tttgtcagtt catctttttt caaaagtttt tccttccttc
     3181 acgcattttt tcagttgtta atcataaagg ttcacataag gcaaataact cgtaattgat
     3241 ttttcgagat atttaacagt attttttatt gtgtaatcct ccttatgagt agcaacgtaa
     3301 gataggtgat tgacaactga ttcaaatcct ctatctttgg ataaacgatc ataaagcgga
     3361 taaactttat tctgaagtcg gctcataagt ttaagatcgc ataagtcggc agaactgatc
     3421 aaaaagtttt tgattagtaa tttagtgtat ttgctattca tagctttcaa gtacgattga
     3481 ttcattttct ctgttttatc ttctgcgcct tttaaataag cagggtctct ttgttcttgc
     3541 ttataaggta ccggaactga catggtacgt gtaagcaaga actttatgtt tgtataagta
     3601 tgacccttct tgataagctc atagtcaatt ttaaaatcag tgtatttatt cagttgggca
     3661 atcggttctt taattacacg tctttcaaag tctcgaaaag ctttatattt tttctcaata
     3721 ctaaatttct ttcgtaaatc tgcaatggag aaaacaggat tttttaatct atcaagtgat
     3781 tttgtactcc atgttaagtt atctctattt gcttcataca gattaaaatc tttagtgaat
     3841 aattgaaaaa aactgatcgc atacttgctt gtcaagttaa ctacttgatc aaatgtatat
     3901 tgcgtaaaat gattttctaa gttcgttaat aaaggcataa ttgaactact aaatttaata
     3961 gatactgcat cagaaaaatc attgaattca gtaatttcta gcggtgagat gactttgtat
     4021 tcagtcttac cgttaggttt agttctttga attttaatga cagattgctg atgcaatttt
     4081 tcaataactt gttttaatct tgaatgtttt gaagaagatg aactagaaga catcaacgca
     4141 aataaagcct ttttagatac tttgacggta ttattaaaag gcggattttt agggtcaaga
     4201 tgtcctatag cgtaaagata aattttttgt ggcatagcat ccatttcaac aatgctatct
     4261 acaagttgat tcgataaagt aacagttaaa tcaaactttt ttagattgct ttcggtagtt
     4321 tcaaatttcg ggtttacatt cttttctgtt attttcatac gattgcctct tctttctaag
     4381 cttggtacga gcttaaaaac ttggaactgg tacgattcca ttgaataagt atagacctgt
     4441 tttcgatacg ttacaataag aaatgtatcg taaatttata acataactca atgtcagtaa
     4501 aacacatttt attttttcca atcaaatttc attgaaaata cagcagaaat cgctatttta
     4561 gaacattttc aaaataaaaa tccttttcaa taaaatacga aattataaaa attaccaaaa
     4621 ttaaaaaaca aatatttttc tagtttttag aaataaattc acatactttt tctttttttg
     4681 gatatattcg gaataccaca tcgctttttg tccacgtttg catcgctttt tgtccacatt
     4741 cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca ccctaaaatc tatttaagat
     4801 tgatataaag cctttttgga ggttgctaaa gttttaaaga ttaaaatata tattataaat
     4861 ataaataaaa ggggaagctg aagcttcctc cttttccttt ttttcaatta ttcaaagtta
     4921 cacaagcaaa ataaactcct agatagtgtt agtttttttt aagatttttt atttttaaga
     4981 ttttgaaata agagaggagt aatcctcctt tattaaatat ctgttaatat atctgttaaa
     5041 tatctgttaa tatatctgtt aggggaggct taatctcttg ggagagtaag tattaaaggg
     5101 gtataaaggt aacacttttg gacagtaaag gtaacaaaag tggacagtaa gggtaacagt
     5161 tttggacagc aaaggtaaca aaagtggaca tttgaggtat cacactagga taagacgtta
     5221 ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa ttagattagt aacaaatctt
     5281 tgttgactgt tacctttagt tgtgatttaa tttaggtaac aagcagaaaa aattgtttcc
     5341 tttaattagt accagattaa catgattaaa ggagcgcaaa aagaagattg gagagtaatc
     5401 aatgtctcaa gaagttaccg aaaagattat tgatagtgat gtagatttag tatcagaaca
     5461 aacaaatgca attgtgcgtt ataacaatgg tctgaataca acagctttgc gaggatttaa
     5521 gcctgttgaa atggatgtgt tttgggctat tttatcaaaa atgaaacgtc aaggaacagc
     5581 agaagtaact tttgatttca acctctttcg ttatttggtc aaatacgaca gaagagaaaa
     5641 ggacagtttt tattctgtgt taaaagattt gagtgacaaa ctaggtaatt taacctaccg
     5701 ttttgaagat tcagacgtct atgaacagct gtggcttttt caaaaattcc ggatagataa
     5761 acagaaggaa acagttacaa ttaaggcaaa tgaggaattt gaatttatct taaactcaat
     5821 tggcaaaaac tttactcgtt ttgaacttga taattttaca aaactaagaa gtagctacac
     5881 aaaagaattg tatcgtcatc tcatgcaata cagaaataaa gagtggcgaa atggttattg
     5941 ggtagtcaaa gttgaagatt ttagaagagc attaagtata ccttcaagtt atagaatgac
     6001 aaatattgat aaaagaatac tagagcaggc gaaagaagaa tttttagctc ctgatgaaaa
     6061 cggaatacag gtatttgaaa cttttgatta tgaaaaaatc tatgcaaaga aaggaagaag
     6121 agttgatcgg ctcagattca cttttagtga gccagaatca aacttgccaa ccatttcaat
     6181 gcataattgg ttagaagaag attgagaaga agcaggttgt aattttaata caacctgctt
     6241 ctttaaaaaa gtatgggtaa cacttttgga caataaatgc aagaagtttt tttgtaagca
     6301 agtttcagtc taaataatat ttgaaagaat ggctaatcta tacaaatttt aacaactatg
     6361 ggtaacactt ttggacaagt caagaaaaaa aacaattttt cttaatttgt tgatcgacta
     6421 aaggtaacac ttttggacaa cacagaataa aaagtccggt gatatcagtt ttggtaggaa
     6481 acatcttaat atctttgtga gattgaaggt aacatttttg gacaagtcaa ctatttgtta
     6541 aaagttaata tagagtaatt agataagttt tttcttttcg tctgataaat ggataatatt
     6601 agttatttta ttgctaatta gtctttcatg atgactgaaa tagctaaaat aaatatctcg
     6661 ttggataatt gctgatactt taagggatat ctaaatttgc tgattgattt atttatgtta
     6721 ttatgatatt ataataacat aaatgtgtta cgcccgaacc tcattttaaa aaaatgaagg
     6781 ggtgagacaa tgaatgctat taattggcat gaatttgtta actatatgga gagcttacta
     6841 gatgatctaa aaagttttag tattgctgat gcagtaaagc atgggttacc agttagtaca
     6901 acgatgaaat tacgaggatt gaaaattcca gatgaaatta ttaagctgag aactaaaaca
     6961 attattgaaa ccgcaaaagt cgttgacaat atgaaccatg aactaaaagg caagaaaaca
     7021 acttacgaga aagattttga acgtttttta aaatctgtta ttggaaaata tctaggtaaa
     7081 aaatctttca gtgaattgag agatgaagag attgtaccta ctccaatggt tagcaaaatc
     7141 cgaaaaaaca aagccgaggg gaattactat cttggtacaa tagcaaaaat cattgattat
     7201 ttggaaaata aagagcaaaa gaaaggaacg aagaaaggaa gataattttt gtgttaaata
     7261 ttaatgggga agaatatacc gaaatgattc aaaatcccga acctagaatt accgaaaaaa
     7321 cgtctgattg tttgctcttt tatgaaaggg ggcagacaaa ctaatggaaa aaaacgtcaa
     7381 ttcaacatct accaaagatc aatacagtat tattggaggg ttgcttggtg ataaggataa
     7441 gagaacacgt cacggattac gcattatgct tgcaactttt ctaggagtgc tttttttatt
     7501 aacaggcttg ggtagctaca tttatcaatt aggaaactat aattattatc tcgtgaacaa
     7561 tacaacacct ataggctctt cattggagtt tgctcgatca ggagcagaga tcactatttc
     7621 agatgtgtgg acagatcaaa acagagatgt aacagtagta aaactcggtt actcgcaaaa
     7681 cgctagaaag ttgttatcta cgcaaggaaa aaactacaac ttatatatgg caacgaaaga
     7741 aaaagatcgt cctaaaaagc ttgaaatgag ctatgggatg ttaagtactg atggggatgc
     7801 tttcttattc ctaaaaggta aattagatga aagagcttat caagttttta ttgcaaacca
     7861 agtatcacta atttctggtg atgatacagc tgacgatgat accaaagtca atgtgaatga
     7921 agcagattca gttacaaaag cactttctca atattctatg aatgatgtta ataatgaagg
     7981 agtaatttct tttgggaaaa aagcaaaacc tgttaatact cctgataata tcaatttcag
     8041 aataaatcca tattcagatt caactaatgt ctatcaagga agctttctta ccaaagatgg
     8101 cgatattgat tattcaaaag ttgtagcagt tacaagtaag aataatgttg taagtaagtt
     8161 agaagatcag ttaaatgctt caaatactaa catcaagaaa attgaagcta caaaagctga
     8221 gtatctggaa cgtttacaac aaaatcctga cgataaagat tcgcaagaaa gtattcgtca
     8281 actagacagt cgaatggaga ccgaaattaa agcaatcaat gatacacaaa aattgattga
     8341 tcaatacaaa aatgctgact ttaacaaaga ttcttttgga gacatgaaga cgacatttaa
     8401 gtacctaact ctgactgact agaagtaaat tattcttatt ttagaggaag tgaacacaaa
     8461 cagatggaaa atagtaccga gttttttaag aatattcaaa ctgaatttaa tcatgctctc
     8521 acaccaatga aaccaccgaa acataagtta gtgggacgtg atgacgaact ccatgagttg
     8581 tcactgatct tgaatagacc ggaaacaccg caagctatct tgttaggtga agccggaaca
     8641 ggtaaaacag cacttgcaag tgaatgggct gaaagacagc gacaagatgg aaagaaagta
     8701 caagtataca aattatcaat cggacaattg cgtgttgggg gagatgatgt cctccaaaca
     8761 cgtttagaaa cattaattcc aagattgaaa gaatatgagc gaattttaaa atctgaggac
     8821 aaagaaaatg aatttgtcct cttcattgac gagtttcata aagttgttag ctcttttggt
     8881 agtggcaata aaattggggg tgattcgcta aaagaaccgt tagcaaatga atcacttcaa
     8941 ttgattaccg ctactacaag aaaagaatat gaaaactata tcaaaagaga tgatgcctta
     9001 gggagacggt tacaaccgtt agaattgaat gaactaaaaa gcgatgttgt cgtcgatatt
     9061 ttgagacaat ggctaaatac atcctctgtt aaagatgatc ctcaggcttc ttttaaatta
     9121 tcgcaaagga ttcctgatga attacttcaa gaaatcgtac aagcaaacaa gctttatcgt
     9181 gaggatttgt ccgaaccggc taaatcaatt gccgtaatgg aggatttgat tgcaaaagat
     9241 cgagtagacg gaaatgctta taatcgagaa agtctattgc aaatatttgg taagtataat
     9301 attcagctag attctgattt tgatatggaa aaacttcaag aggaattaaa gtccataaaa
     9361 gggcaaccgt tagcagttta tactataaca atgttgttta aacgtatgaa gcttggattt
     9421 gatcgtttta aaggtcgaaa gcaaccaaaa gcaactgctc tgtttacagg atcaacaggg
     9481 gtcggtaaaa ctgcaatggc taaagccgtg gcaagaggac tttatggcga tgaaaataaa
     9541 gttctcttag ttaatatgcc ggactacaaa acaaaagatg atgcaatgcg ttttagacga
     9601 ctcataggta ctcatgtaag gcatgatcct tctgcgatta ttgtgtgtga tgaatatgag
     9661 aaagctggtg tagaaacact cgatgccttt ttatcaattt tagatgaagg taggataagt
     9721 tttactgaaa gaggatggga tgatcgagaa gttcacgaca ctgtttcatt gtcaaataca
     9781 attttatttg cgacaacaaa tgcaggtgcg gagatgttcg attcgttaaa cagatatagc
     9841 tcactatctc gtgttacaaa caaggaaaat tctaatgaat atagcactga actaaaaaga
     9901 gaataccgaa aagtcgcatc agggatcaaa caagccttga ttggtgaagg tctaaagcct
     9961 gaagttatta accgatttgg acgaatagtt ccattttggg ctttatccga acaaactctt
    10021 ctggaaattg cttataatga aatgttgaag agtgctgaat tagtacgaaa acatttaaat
    10081 atagatttac gtcttccacc taggaaagaa agagtatatg gtcaaaattt ttatgcagac
    10141 gatctctcca tgcacttagt ttttgacatg gcttcacaag aggattctaa atcagggggt
    10201 gcaaggaata tcttgcattt agttgaatct gagtttgaag aagcgattac tgaagcaata
    10261 attacttatc caaacgcaaa aatattcgat gttaagttga gtgaagccgg agcgtttgaa
    10321 ggaaaaggtg attctaatgc tgaaattatt gcaaagcctt ccgttgctga aagtaaagaa
    10381 aacaagattg tattttcgat tggaggggcg tagctatgaa attcaagaaa tatttactac
    10441 aaaaccttag aataaaacga tgggtcgtaa gaggtctagt tattggagga gtattagcag
    10501 ttactttagg cgctcctttt ggaatgaatg atccaactcc tgacaatcaa gatagcaaac
    10561 aagaaacagt tataaataag aacgttgcat atgcggaaag taaaccggca gaacaaggaa
    10621 tgtggacact atatgctcgt atcctaatgg gagataaaga agttaatgat agtgccgcta
    10681 aaactggaca aatgaatttg ttaggacaag gtggtatcag tgccgatttc ccttattctg
    10741 acatttctaa acaaggtaat tcgattgcag ggggtaatgc aggtactcaa ctagcatcta
    10801 ctttagcaac ttatagttac tacggatata ttgaaacagt ctcaggaaat gctattgcca
    10861 gtcaagcatc atccattcta ggaagtattg gtaggacgat tggtagtttt atcggatcaa
    10921 ttagtttaat cctttctaat attgctgaat ccataaatga actccttgga aaatttttca
    10981 tgagtatgaa tatattttct cttatggggt ggggagaagg tggatcggat gtatctaatc
    11041 cggtatctaa agcattaact agcttgctta acaatttggg attaaataat gaattattta
    11101 aagcgatagc aagcttatct atgctctttt tcacagcaat actaggttat caaggaatga
    11161 aattattgtc gaaagatggt gttaactcga agggattatc accagtactt gaaaaatgga
    11221 ttgttcgtat ttttgtattg tttatattct taccagtggc aggatcgcta ttgggagttt
    11281 atatcaacgg catccagtca gcaaaaaaag atggagatat accaacaaat gttgtttctg
    11341 actacatcat taacacaaga ctttgggctt ctactcaaaa tcttgctcca tcatctgata
    11401 ctggtggtga tgtacctgac ggaaagccta catccgaaat caagttcgtt gatacagatt
    11461 atgaaccatc taaaaaatct gcaatgataa gagcgatcaa tactagcagt tatgccagac
    11521 aaaataatga tctagattct aattcagtcg gtatggctct attaaaagaa tggtcatcga
    11581 atgatacttt caatattaat acttatgccg gtgatgttaa tgtcgccgaa aatgatgctt
    11641 atatgggaag agcgcctgct gtttggacag gctataattt aggggatgga aaagaaggaa
    11701 gtgcaattaa gaaaccaacc aatttaaatg gatatatttg gtcagcaaat caaaatgtga
    11761 cagacgacaa tcgcaagcct gatggaaaag gaacagcgca taactttgat gctactcaac
    11821 cttggggcgt tgctaacaat gcaactttta gcacacagtc ggtagcttta ttgctccaat
    11881 cggcacttga tacatcggga gctaagtttt atgcgtataa tatcgctcct tcaggaattc
    11941 aaggcagtgc taagaatatg tcaacagtaa aaacagaatg gcgtacagca tccttagttg
    12001 gtcatggagc tatgggtaag tttggctcgt ggctagcgat gaacgctgaa tcaatcgttt
    12061 tagcagttct ttacttagca tgtgcttggg cgatgttcaa tgtgaatatc gtgaaagcat
    12121 ttaaacgtga atttattcgg atttgggaag tagttctaac aggatcactt gcatcaggag
    12181 caggtgtgat ggcaatttca ttaggtgttg ttacaacatc tctgatcgga ctacaattac
    12241 ctatctttct gattactttt attcgtgcat ttggtgatac tgctgtggga ttcattccta
    12301 atacattagt tggcgatggc gtagcaaata ttgttcgatc tgctatgatt ttaggtgtag
    12361 gatatctttt agtctttaaa aaaggcgaaa aagtaaataa tttgattaat atgcttattg
    12421 gcttaccact tgaagtagcg tttgggtttg atgaacgtgc taagttatta tttggtaata
    12481 gaaatgatgc tgtgcgagtt gtggacggcg tttctaaagc aatcaagaaa agccaaggag
    12541 ctaaaaatcc ctattatgat ggtggcaaca gctcagtaaa agatatggtt gcaggaggtc
    12601 ttggagcagt tggagcgcaa aaaatggctg aacgtttgaa caatatggga gatagcaatg
    12661 ctgatgctaa gtccattgct gataaagtaa ttcctgaaac accagaagga atgtcattca
    12721 acggtggcgg agttggttcc acaatcaata atacctcttt caaagattta gttcgtcaag
    12781 atcaactacg taataactcc caacaatata ttgatcattt aggacgtatg gttgatgaaa
    12841 acggaaactt tgtcgatgaa aatggtgaaa tattaaaaga tagtaaatac ggtaaagatg
    12901 ctaaaggcaa ctttattgac gaaaaagggc gtttggtcga tgaagatgga aaccttatag
    12961 atactaatgg taaaccattg gaagatagtg aagatggtaa tattggtatg gatagcgaag
    13021 gacgtttaat tgatgaaaaa ggtcgcttag tcgatgaaga tggaaatctt gttgacgaaa
    13081 atggacgcag gttgctaaat ggtgattttg gtcatgatag taacggacgt ttagttgacg
    13141 aaaaaggtcg tttagttgat gaagatggaa atcttgttga tgagtttggt aataaacttg
    13201 gtaaacacag cgatggatat aatgtgaatg gagatttagt cgatgaggat gggaatatca
    13261 ttgagacgag agatgcgctt gtggcagatc gaaatagaga actcgaacaa caacggcata
    13321 cgccaattgc tgttgcaatt cctgagaatg aagaagacga gattacttta gatcaaactc
    13381 atcaagaact gaggaatgaa atgagtggtg aggacgtgtc agacagagtg catcgggaat
    13441 taggagatgg agcatctggt gaaagtcagt caagagagct taacagcaac tttgataata
    13501 gtgctattcg gaatgacaca gtacttaaag acagagaatc gctaacaaaa gaaacggata
    13561 aagaacaatc agatgtgaag aaaaagttca ttcctactcc taatgttaat caaacagata
    13621 aatatgaaac aaatattgga tcaggaatgt cgaaagacaa tcaatcaatt agagaacctg
    13681 aactgacaaa taaaaataat agaacaaata attctaaatc tacttcagat aattcaggga
    13741 aatctaatca atcaaccaag aaaacatcaa cagctaaatc agccgttgaa tatctatcaa
    13801 aaactggtaa aaatgtttac acctcagcag ataaagcttt agcaaaagct ccaaaaggta
    13861 gtctagcagg gataacctat gaagctaaaa acgaagctat taaaataggt aaaggaacgg
    13921 ctaaggttgc caaggctact gctacaggag catccaaaac tggtaaagta gtgaagaaat
    13981 ctaccatgtc agtcaaggac tatattgtaa aatacggtaa tcaaaaatca actgaaaata
    14041 agtctgaaga cgagtaaaaa gaaggagcat tgatttgctc cttcttttta ctttgatttc
    14101 taactgaaga tgtcaaggaa aatttctatt tatgcaaaaa aattatttta aattgtctat
    14161 aaatgaatta aatatttact gttcaaagtt gtataattaa aaagggctta tgctggtaaa
    14221 gaacagaatt attatctaaa caaaaacttg ataaaccagc atttttagcc aataaaaaaa
    14281 gtgaaataat ttaatggaaa atctctttta atcatcgaaa atttatgtta ttatgataac
    14341 ataaagatgt aaatatcgag acgtcttttt cttagttaga tcattgacaa gtgaacaaat
    14401 aagatttgtc gatgatataa ttaacttatt aaaaatatta aaaaggaggt ttttatatgt
    14461 tcgattctat taaaaatttg gttagcggaa tgacgcctga tatgaaaact ttagtacaag
    14521 ttgttttgta tttcttggca ttcgtttttt cgttgaaacc aggattcact gcaatgaaat
    14581 tttttggtga tgcaaaatgg ggagatggat taaaatatgt tggggcgatt attgcaatct
    14641 ttattattcc tgtaattaca accgttatgc tttacacaat gggtactaaa acaggtaatg
    14701 atttgaacca aaaagctaat atcatatcag cacttattcc agttgttggt atctatctag
    14761 tttcaaaatt tccaaaaaat gaactgttag gttaaaagtg aattgaacaa atttggataa
    14821 tatgccgatt tgttcaattt actttttacc aaaaaactac gctccaagcg tagtttagaa
    14881 gtgagggaag aaagtaaatg taagatggtt tttgatagag aaaaaatgtt agctcacgcc
    14941 aacgaagtat taatgagcag tctgaaaggt accgaactgg caaagataat gaatatgaat
    15001 gttaaccagt tctatgatta tcgaaatggg agtaaaaaga ttgaaaaagc cagacttgaa
    15061 acgcttatta aattcgaaaa agcatatgta tatatgctag ataaacagaa aagaactatt
    15121 gactgaaaaa aggagtttta caataatggc aaaattcaaa gatattaccg aatctattaa
    15181 ttatgtggca gaaacagttc ctgtagttgc actctctttg aagccacaat tactcgaagt
    15241 gtacaaaaaa gaagaaaaag aagcgaaggc aaaaaaagct aaagcaaagg tatggaaaga
    15301 attcgatgcg ttgctgacca aagatttacc aaagaaaaat tatatggtaa ttgaagatgt
    15361 acaaaagctc aacagtgagg gagttataga tgaggatgat gctattctcg attctgagaa
    15421 gtatgtcgtt ctattgaagt caagtttgaa ttatgtagct gatctaccag aaaaatttaa
    15481 agagaagcta ttagcaggtg tccgttttat ggatagtagt ggtcaattat tagttattcc
    15541 ggatgatcct aatgtgtttg aatcagaagt tgaagaagct acagacttct atagcgcagt
    15601 agagcaatta gaagtagcta ctaatggatt agaacgaatt gctgagacct tcaactcttc
    15661 tgaagaagtt gaatttgaag ccgtagcaga aaatgtacca gatgaaaaag aagctctttt
    15721 tgtggatgat aacaaaccaa ccgaagcaga aaaccaagaa gtaagcatag aagaaacaca
    15781 aatacaaaca gaaacctacc aacaaccaaa aattgatgaa aaacccgaat cccaaagtga
    15841 caaagaaaat tatgtttatg aagctgaaaa aatcacatct gaaagtgatg taccgaaaaa
    15901 cttagatgct gattttttag aaaaaaattt tgaacagatc attgctgaca aatttcaacc
    15961 tgtagtttta gatgagatta atgaccctgt cgatttaaac gaggagatgc tatccatacc
    16021 tgagttgaaa ccattgctgt ttttagctaa agagagtatg aacgctcgca tctctattgc
    16081 taataataaa atcaacagtg aattaaataa gacgaaggag caattgatcc tcaaaggaaa
    16141 gcaaatgtta gctgatgagc ttttgcggat agaggaagca acctcattaa atggtcaaaa
    16201 tcagtttaca aatacactat cggttgctaa agagagctac gaagcagaag catctaaaat
    16261 tgatgattta gtgatggaaa aatccaatat cattcgtaaa gattatgata tggcgaaaca
    16321 ggcttatttg gatgctgaac taaagaaact agagcaacgg tatgatgctg agcatttacc
    16381 agaagttaaa caacaagaag atgaatatag aaaagcaact gaagaaagtc ttgatcaaca
    16441 gtaccaagaa gcaattgaat ctattaaaaa cgatgcattg aaacaaagcc aagaagcaaa
    16501 agaagttgtg gtggaacgaa ttatcaattc attgaaacca gaaattgaaa aatcattaac
    16561 agacgtgcaa gaaaaaactt tagagattgt cgaaaaaaca aagtcagaaa atgaagctga
    16621 gttccatgat acaaaagaga aaacaacaga tgtggttgtc aaatattttg cgaacaaaga
    16681 agaagttgaa aagcaggtag ctaacaacac gaaagaatta gaaacaaaac ttgtagagca
    16741 acaaaaaact atcgaagaat ataaagatcg aatgaaagag gtcaaagatt tagcagaggg
    16801 ttataaatta aatagcgaaa agttggcaga acaaagtgaa gctttaagag aagacttatc
    16861 tcgttcccga agtgatctag ctcaaatggc agaaaaaacg aaatcattag cagttgctac
    16921 agaaaatcaa tcaagttcaa aacgtgattt tacaagtaaa aagatggcta ctatcatttc
    16981 tgtcgctgca atggcttgcg gaacgatctt gggatcgtcc tactttatta gtcataataa
    17041 tcaacaagca caggctcaaa ctcaaacatc tatttcgaga gtggaaaatt cgattgggac
    17101 aaatacgaac tctgcttcaa caaatagtag ctatgaagca acaccaacta cagagttcaa
    17161 agttgggaaa gatgttgtca tcagtattga tgggaaaaca actaaggctg aagtgatttc
    17221 tattgatgga gaaaaagcag ttgtgaaaac tccagacgga aatcagtaca aaattcaagc
    17281 tcacttatct gataataact aaggagggct attatgcgag aagaaaaaat agtttatcca
    17341 gtaattcaaa acaatcaaga aaacatgacc tcttctcagc acttacagga agcacagctc
    17401 gtgtatcagg taaatgaact accacaaacg aacgagacaa tgagcaagtc aatatcttta
    17461 attggcttgc ttattgttgc aatagtgata actgtaggtg tttaccgtaa gttgaacgtg
    17521 aaaggaagta tttctgatgg cagataactc aaactatgga tttaaacata acttacatca
    17581 aaaaccttct gaaacaattg tcagagttga atggcttgaa aagcatgtca atagcaccta
    17641 ttctgttaca gcaaaaagat taattgtatc agctatagtt gttgcagtcg ttgttcttgg
    17701 atcaattgtt tacttagttt ctgatacgat taaactgaaa tttatttttg ttccaacgtt
    17761 attaatgata attggatttc ttattctaat tagccagtat atgtttattg atccatctag
    17821 tggttcattg atttctggtt ctattagact gttcaaatca gtagttaatt cagcaagagt
    17881 atctatgggg aaaaagaaac catttcgagg cacagagatt ttaaaagtga aacaagatgg
    17941 aacaatcgta tatcgagatg ggagtgtagc attaatgttc aatctagatg gggctacatc
    18001 actaaccgct tttccagaag aagttatggc tcaggaatcg aaatcaatac aatatcaaaa
    18061 taccagagat cgttccttag ctgaaatcaa aatcacttca tctcaacctc agaatacgga
    18121 aagacaaact cgaaatatga aaatacaaaa acgaaatgca aggagtaaca aagcagtctt
    18181 tgacataata gatcagcaag aacgatttgt atcagaatat attgatggtg tgaaaacaac
    18241 agtaatccaa tatcttatct tgaaatctca atctaagatg gtattggatg aaggtgtgga
    18301 acgtttagaa aactatacga gtagaggact ttattatagt gtcactcagt tgtcagctaa
    18361 acaaacaaaa gaaatattat ctgatattta tgcactgaaa tagaggtgag agattaaatg
    18421 gaaattatgg gtagatcaca actattaaat ttcaaaaaaa agagaaaaac taaaaaagaa
    18481 caaaaaacta gaaattcaaa ggtagtgaat aatacctttg ttagtgattt aacatcttta
    18541 gttggttatc aatttacacc atcttacatt aggacaggta agcggtgggg aacaattgtg
    18601 aaattagtga atacttatgg aatgaataga aattatggat ttggttggat ggttaaagct
    18661 attcctgaag tgagagtaga aggggtaaaa gcctatctaa ttgaagcaga taagccagtg
    18721 tcacaaaaag atcaacaaga aataatgcac gagaatgttg ggggatcaac gaatagttat
    18781 gaaaataatt tagaaggaaa aaattcagat gctgaaatta gcaaaactgt tatgcggatt
    18841 atggatttga atacagcatc tttactagac agcatggcaa ataaaattat tgattggaag
    18901 ctagaaatcc tattagtatc cgattctcct aataaaatca gcgaacagct aattaaactc
    18961 caacggttgt atgatgaaaa aatgtatgga ttaaagctta tgtcatatgg aggagatcaa
    19021 cgtgatctgt ttgatagact tttaagtgaa ccaatcggga atgtctatga gtatacgtct
    19081 atgagttcgg attttgcagg aaatgatcat gcaattcgca aaggtttaga tgatgcgaat
    19141 ggatgggcta tcggcactat gtctactagc tacgcaagtg gtgtagcttt tatggcactg
    19201 gatgattctt ttacaagtaa agttttagtg gcatcacaca aaaacgctac aattaaaggc
    19261 tatgatgaaa aaatctcagc atcatcactt tgggggcaat tgattgctaa taatgcaatg
    19321 gcaaatcata aacgaacttt tcatattgtc ttaaataaat ttgattactt tggtgatcca
    19381 actggtagaa catttgttgc caaaccttcg ttaaacagtg taatagaaaa ggttaatttg
    19441 gctgaagaag gtcttaactc tttggagctt tttggtaatc aaaaggacat tggtgattta
    19501 tatagacgga atctcgataa actaactcag attttttatt tgttagcagg cagaacatta
    19561 gattctgcaa ctagagtttt attgaaaaaa gaattaggta acttttatat aagtcagaaa
    19621 ctttgggata ttaattatga agaacgttca aaacttttgg tgaacagtag tgatacaatg
    19681 ccagttcttg gggattttat tagctatttg actaatgctg aaacaaaagc caaaaaatct
    19741 ggtactgaaa aagaagtcga tcgtattatt gtattaaaag acacattgag aacagcatta
    19801 gacagcttct ctaggatgtt taatcaaaga acgacactga aagaaatcgg taaaagtgac
    19861 aaattacagt tttactataa cttaggtgat ctaagagcag aaaccaatgt tatggaagct
    19921 caatttttaa atacctttga tttcatcaca tcacaagcaa aaaaagatga tattgttatg
    19981 attcacggct tcgataagtt atctattgaa accgttgggc taattaaaaa aagattaaca
    20041 atgcttgaag aagatggaat tcgtcttgcc tttctatttg atacaatcgg ttcaggagaa
    20101 gacaatgaat acaaagaaga tattgaaaca gctgatatat tcaatacaga tggaaaaatt
    20161 taccaaagtt ttgaaagtga ttttaattac actatcattg gaaagatgac cagtggagag
    20221 ttaatgaagt atgaaaaaaa agttgctcct gctagaaaag gagaagatga tcaaaaactt
    20281 tctgttgatt tgagacagta tttgacaaca ggaagaagta ataaatttca gatcaggaga
    20341 ccagtggatc tgactaataa ttttgttatt ggagagttta ttctttaagt tttatctttg
    20401 attttgagtg tataaggaaa ggataagaaa aaacatgtat atttgagact aaaaacagtc
    20461 ttagagaaca tggtacgttt tgtgaaaaga gaactataga aatttcataa agtgaaacct
    20521 tattgagtga aataattata gtggaggtta aataatgaaa ccaaacataa aaattttaga
    20581 tcgaatattt ctaggtagag acacagaagt aatcctaatt caacatgaag aaggatttga
    20641 agttagtata ggtattcaga aacttcaaaa acctcattat tgtaatcagt tgtataaaaa
    20701 ctttactgat gaagaaaaag cgagagtttt ttttaatgaa gtcaaaggta tgagagaaca
    20761 gtatgaggtg gttgaagcat gaataagtta ccagaaaaga accgtgtatt tagattgaaa
    20821 ggtgatctca gtcaatttaa aatagatgag aatggacaat tttttgtttg gatgcgacat
    20881 gagcattgtt ggtgtaatcc ttttgtgtta aaaaaagaaa atgaaatcag aactaaaatt
    20941 gcaaatggtg agtgggatta cctcttcaga tgggaagatg aatcttttat agcaaatgat
    21001 atggatcatg catttgtctc atttctggat aatacatggg gtacttggta cgattgttac
    21061 ctagaaaaat tgggctttca aaatattgat attgatgcta ttgaatgtct taaagaagtc
    21121 gatcaaaagt atgaagaaga accctattat ctcttgcaac aaaaattagc caataattca
    21181 gtgtttcagg tagaaatttg ggaaaatgaa gatggaactt atgaggggct agcgcaagaa
    21241 atacccttct cagataggaa gagttcgatt tgaaaagagt atatcagcaa tttttatgaa
    21301 gtgaaagcaa gcttacaata tagtaatgga aaattgtggt tagctggaag gtatattcca
    21361 gctaaccaaa tcttacgttt tctgggagag tggcgtttta aaacaatttc ctaggaaaaa
    21421 tagttaacat aaaaggaatg aattgttatg aaacaatatt taaatgaatg gaaagcgata
    21481 gaggggtcat tagtaacgca aagaataaag gaattacctg attgcttaga gaaggatcat
    21541 ttgtttcaaa tacgagaaat gttgaaaaaa gaacagtttg atccagacca atttttagta
    21601 gtcgagtatc caactattgg agtgtactgt tgtaaccata tcaatgatgg aaaatatttt
    21661 attattcagg agtatggagg acagttaact cctttctata ctacatggga aatgagtgaa
    21721 gaaggaatca ataattttcc atgtgaaagc atcgaagatt caatttcaca caccgaatgt
    21781 taattgatta taactataaa aaaggattga acattatgtt caatcctttt ttcaatgaaa
    21841 tgttttccgc catttcattg tttggtgcga tttctatttt attatatcaa aataaagaga
    21901 gattctaaag aaagaagagg gaaatatata aatcaagcga cagtatattt attattaaat
    21961 gctattgtga gaatataact aattagttta atttgaaagt aaatatacgc tataaaagtg
    22021 catcttgtgt tataaaaaat tattttattt tgtgaacata atttaacaat tctttctcgg
    22081 aattgatttc ttttcttaaa tagtcttcag acttattaat aatattatct attatgtcta
    22141 gatacatatc acttttcgat tcagctaatt catacagttt ttttaattct ttatccttta
    22201 attgaaaacc ctgatccata gtcactgcat ttggatatag aagcccacta taatgtttac
    22261 gtaaattcac ttggaatttc tgattataaa agtctaacag attagaaata aaaggcggaa
    22321 aattactgtt tttgtaaaat tgattatttc catttaggtt gtatagtaat ttgttaaatt
    22381 gctcctgatt taatttattg taaatccatg gtaatacttc catatcctta tgggagcgtc
    22441 aataattttg tgtaaataac tcgtccttct gcaaaataat gggttactca gtaaacattg
    22501 aagctaatgt atccgtcact tgttggaagc ctttatggct tctatttaag aatttttgat
    22561 tgtatgtatc aaagatggat actaggaaac gctctagtga ttcttcat
//
LOCUS       DACJGB010000050        22301 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.050, whole genome shotgun sequence.
ACCESSION   DACJGB010000050 DACJGB010000000
VERSION     DACJGB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.050"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            5..403
                     /locus_tag="IYI34_001925"
     CDS             5..403
                     /locus_tag="IYI34_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAR0559788.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            387..1082
                     /locus_tag="IYI34_001926"
     CDS             387..1082
                     /locus_tag="IYI34_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAR0559789.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1676..2563)
                     /locus_tag="IYI34_001927"
     CDS             complement(1676..2563)
                     /locus_tag="IYI34_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAR0559790.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2748..3587
                     /locus_tag="IYI34_001928"
     CDS             2748..3587
                     /locus_tag="IYI34_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAR0559791.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3783..4757
                     /gene="sgrA"
                     /locus_tag="IYI34_001929"
     CDS             3783..4757
                     /gene="sgrA"
                     /locus_tag="IYI34_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAR0559792.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5055..5411
                     /locus_tag="IYI34_001930"
     CDS             5055..5411
                     /locus_tag="IYI34_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559793.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5414..6571
                     /locus_tag="IYI34_001931"
     CDS             5414..6571
                     /locus_tag="IYI34_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559794.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6597..7037)
                     /locus_tag="IYI34_001932"
     CDS             complement(6597..7037)
                     /locus_tag="IYI34_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAR0559795.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7197..7703
                     /locus_tag="IYI34_001933"
     CDS             7197..7703
                     /locus_tag="IYI34_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR0559796.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7871..9784
                     /locus_tag="IYI34_001934"
     CDS             7871..9784
                     /locus_tag="IYI34_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAR0559797.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9789..10790
                     /locus_tag="IYI34_001935"
     CDS             9789..10790
                     /locus_tag="IYI34_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR0559798.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10992..11198)
                     /locus_tag="IYI34_001936"
     CDS             complement(10992..11198)
                     /locus_tag="IYI34_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAR0559799.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11844..12692)
                     /locus_tag="IYI34_001937"
     CDS             complement(11844..12692)
                     /locus_tag="IYI34_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR0559800.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12856..13758
                     /gene="gnd"
                     /locus_tag="IYI34_001938"
     CDS             12856..13758
                     /gene="gnd"
                     /locus_tag="IYI34_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAR0559801.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13760..14761
                     /locus_tag="IYI34_001939"
     CDS             13760..14761
                     /locus_tag="IYI34_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAR0559802.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14758..15042
                     /locus_tag="IYI34_001940"
     CDS             14758..15042
                     /locus_tag="IYI34_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR0559803.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15075..15872
                     /locus_tag="IYI34_001941"
     CDS             15075..15872
                     /locus_tag="IYI34_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR0559804.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15886..16719
                     /locus_tag="IYI34_001942"
     CDS             15886..16719
                     /locus_tag="IYI34_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0559805.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16737..17594
                     /locus_tag="IYI34_001943"
     CDS             16737..17594
                     /locus_tag="IYI34_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR0559806.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17616..18125
                     /locus_tag="IYI34_001944"
     CDS             17616..18125
                     /locus_tag="IYI34_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0559807.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18162..18563
                     /locus_tag="IYI34_001945"
     CDS             18162..18563
                     /locus_tag="IYI34_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559808.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18675..19028
                     /locus_tag="IYI34_001946"
     CDS             18675..19028
                     /locus_tag="IYI34_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559809.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19062..19505
                     /locus_tag="IYI34_001947"
     CDS             19062..19505
                     /locus_tag="IYI34_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAR0559810.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19522..20607
                     /gene="uxuA"
                     /locus_tag="IYI34_001948"
     CDS             19522..20607
                     /gene="uxuA"
                     /locus_tag="IYI34_001948"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAR0559811.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20600..21313
                     /locus_tag="IYI34_001949"
     CDS             20600..21313
                     /locus_tag="IYI34_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR0559812.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            22065..22265
                     /locus_tag="IYI34_001950"
     CDS             22065..22265
                     /locus_tag="IYI34_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAR0559813.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7682 a         3427 c         4620 g         6572 t
ORIGIN      
        1 agagatgacg atctttgaaa cggttgtaac agatgttggt atagaagcag aagtattcag
       61 gaaagaaaaa ataatgattt tatttggaga tgaagcaccg gaaacattaa cagattattg
      121 ttataagatt gaattgaatc aggtaagaaa agaaataact tgtgatatga agctctgttt
      181 tgataaacaa atgtatgtaa tcacagcagt aggagatgtt gtccaaacta atttgaatga
      241 attaggacat atcacgataa aatttgatgg atctcatgag ccggaacttc caggaacaat
      301 gtatgtagaa gataaggaaa tgccgcattt aagaaagggg acaatcatta aaatcgatca
      361 agaggatcag aatgggggaa agctatatgg aaatcatgct tgatacagca aatacaaaaa
      421 gtatttattc ttgtcaacag tttttgaatt taacaggcgt aacaacaaat cctacaattc
      481 tgaaaaaaga aggagcgtca aacttcttct cgcatatgaa agaaatcaaa aaaataatcg
      541 gacagattcc tatccatatt cagacaatag gaagaacgga agaagaaatg atagaggatg
      601 ctcgagttat catagaagaa ctgggaaaag atacgtttat caaggtgccg gtgaatcaag
      661 caggccttca agcgatcaaa aagttgaaaa aagaaggtta tttgattacc ggtacagcta
      721 tttacacaga attacaaggg tatttagcta ttaataatgg agcagattat attgcacctt
      781 attataacag gatgcaaaat caaggcattg atgctatgaa agtgattgat tcgttgcgga
      841 ctgagatcga acgtacgcaa agaccaacaa aaatattagc agctagtttc aaaaattgtg
      901 aacaagtcac tacagccatt cgatctggag ctcatgcgat aacgttaggt aaagagctac
      961 tagatcaagt attagagatg ccctcgattg ataaagcagt gaggggcttc caagaagatt
     1021 gggtgcaaac tttccaagca gaaacgattg gtgaactaaa aaaggtcgct gattacttct
     1081 gaatcaattt ctccataaaa taaacgatag ccttctcgaa aactcgagga ggctatctga
     1141 cgattcaata ttcattaatt taaagtttgg gtgactatta ctaactatta cttatttctc
     1201 atttaaacga tgtattccct aacctctaat acatccttgt aactctgaag ttacgccagt
     1261 tttgaataaa tttcataaaa acaaatgaaa agtgtcgcat aaaaatatct ttcgtgtttc
     1321 tctagtataa cagaataaga agaacaagtc cagaagggat atttttagtc ttttttaaaa
     1381 taacttttca gtcgagtttg agtttatgat agttgttttt gaagcgatac ggcaagtgaa
     1441 aaaataggga agcatgaaag aatctactta atggcgcatt gattcatgtg atagaaataa
     1501 aaaacttttt taaattatat cctcatgatt gtaacaaatc cagtgatttg ttttggtaca
     1561 aataaaaaaa ggccccgatg aagttgtggc aaaagtggtt tgcctcaaaa ataagaagga
     1621 atttccaaaa atcacttctc aaattttgcc acatccccaa ttgggtcaaa tttgtttatt
     1681 tgaatatatt ttttacataa ggatctttaa tagttacatt gtcttttttc atcactttac
     1741 ggattgtttt atccatgaat tccgtatcaa gagtttttgc ttcggtagca attttttcta
     1801 gctcagattt gtatttgtct ttattagaac ctttatcctg tttttttacc atttttacta
     1861 gataatagct ttgctgataa gtcgttggat ctgttacagg aatgatatca gaaacttccc
     1921 cgttttttag tttaaatgct gcttttttta cttcagtagg aatctctgga ttggttgaat
     1981 caaaattcat ttttccatca gtttccttta atgttttatt ttttccataa gcttgaacaa
     2041 gcttggagaa attttctcct tcatttgctt ctttttttgc tttatctgct tcttctttag
     2101 tcgaaaatag aatgatctgt gtactaactt ctggatagaa ttctttccaa gcagtgtcta
     2161 gatctttatc tgttaatttt acattggctt tcaaaccgta ttggaaagcc agcatctgtt
     2221 taaagagttt cttatatgtc tcttctgttt ggccaactgc ttttaattgt tccttgtagt
     2281 ttggaccgag ttttttgatt tgttcattat attcttttgt tacttccttt gaagaaatct
     2341 cttttccgta tacatcatca aatgctttga agacaatcat tttttttaat agtgtttgat
     2401 tatttgattt aacttttggg ttatcataaa aatcctgaac tgtaagtgtt ccacctttaa
     2461 acgttgcaat cgtttcattg gacttgctac aacctgagaa tagtaaaatt gcacatgctg
     2521 aaaggcacat aattagtagt tgttttttca aaaaatttcc catcctttcc ttattaaaaa
     2581 cagtctaaca gaagaattag ggagacagtc tttcattttc gtaaggttgc tgtagatatt
     2641 cttagttcgt aaaaatctat tctctctatg atggagaaca tttacatagt tttcacgaga
     2701 caagaaagaa aagggttgtt aaacttaata taaaggaggg atgaaagatg atcaggatta
     2761 ttcggttgat tggatctgtt ttaatagtct tagttggttt tgttttagga attcttgtca
     2821 ataatgcact tgcggatatg tttattggaa atagtgaatg gattggagca attgccggta
     2881 cggtcggaca tttagctgtt tttttactag ctttatttct tgctagtaag gtcgaaggaa
     2941 aaagaataga agattatggt gtttctgttc gaggaaaaga ctgggggtat ttagcgggag
     3001 gtctcgttgt tggaattgga gtgtttctgc ttattactag tccattatat ttgacggggg
     3061 cgtatcggtt agattcagga aacgcaaaca ttgtgccgct gataacgagt tttattttat
     3121 ttattgcagt gggggcatca gaagagcttt tattccgtgg cttttttcag catcagatgc
     3181 ttagatttgg tccgttaata gcaatgattg gatctgctgc attatttgct cttttgcacg
     3241 gattgaatcc taacatgaca tttctggcag ttttcaatat ctttttagca ggttgctttt
     3301 tcagcgcact tatctacagt acaggaagtt tatttacggc tattggtgct catattactt
     3361 ggaattggac acaaggagct attttgggta taccagtcag cggaacgcaa gaacaaggtt
     3421 ttttctccac tctgatccaa tcgcaaaata caataattac tgggggaaac tttggggctg
     3481 aagcatctat atcaactaca ttattcttgt tagtcttgat tgttggtttg ttactctatg
     3541 catataaaac aaagcggatg aagaaataca ctgctgttta tcaataaaag atgaagaaag
     3601 caaggctgat atcggtcttg cttttttaag tctgtcattg attaaacgaa aaagtaaaca
     3661 aattcaagcg aatacacgac atcttttgtt agattttcac aactgactgt cgtgttctca
     3721 tcattttcat tttatataat tcctttatca aatagaaggt aattatatgg agagtgatag
     3781 aaatgaagaa aacagcgacc gtattatttg ttattcttgg gctgagtata attgcagcaa
     3841 tgattggaat cagtacttct gcaaatgaac gggcaaatga gaatctttca tttactgtga
     3901 aaactgatcg gattgtttat gacatgacac aacaagtcat tactattcca gtcaaaccaa
     3961 acaaaagcgt gaatgctagt gatgtacatg ctgttctaac atatggttgg gatggtaatg
     4021 gaagttcaga aaaagttatt ggggaagttt atttaaaaga cgtacaatgg accgctggta
     4081 ttgagtatac aataatgatt tctgcagaac tatcgattga tgagatcaag agtaaagata
     4141 aagttgattt gattgttttt tatgatggac agatgacgat cacggagaat ttgaaaccaa
     4201 gcagttggac agttgttgga ccgccaagca gtacggatag cacaagtagt gaaagcagta
     4261 cagaaaacac gagcggtgaa agcagtacag agagtacgag cagtgaaagt agcacagaaa
     4321 acacgagcag cgaaagcagt acggagagta cgagtagtga aagtagcaca ggaagcacga
     4381 gcagcgaaag cagtacggag agtacgagta gtgaaagtag cacaggaagc atgagtagtg
     4441 agagtagcac aggaagcacg agcagcgaaa gcagcacagg aaaagtaaat aatgaacttg
     4501 atttggtacc tatcatacca actaaggaac aaaagagaaa tgcagggagc aacctaaaaa
     4561 gtgtgctaaa acagcctgat tttgtttctc gtggaattga tcatcaagaa aaagcgaaag
     4621 aaacagataa tagaaaaagc gatttaccag aaactggtag tagaaccttg aacaagctaa
     4681 ttacatggat gggtgtgtta ctcattttga ttgtaggagc aagctatttt cgttctctct
     4741 tccatcgagt gaaataaaga ataaaagcaa cagatctaaa attgaataaa acgatagatt
     4801 tttgagtgaa tatttcattc gagagtgctg tattgcctgg cgaagttgta gctttgccag
     4861 atctattttt gtttactaac atatttgaga aacaggaaat aacaggaaca caaatggaaa
     4921 ataagagcaa gaataattga caatgcacac tgcgcatggt agtatctctg aaaaatcagt
     4981 aacgaatcta tttgtacgtt attcatgtag ctgtcggaat aagtctaaat aatagataaa
     5041 aaggagtatg tactttgttt gaagaattta ttgatatcaa tgaaaggcaa gtatatcaat
     5101 ttttgaacta ttgttatgaa agagacgaga aattatatgt ggtgaaggat atcgctttag
     5161 atctaaacta caccctggct aagatgaatt cggtgattca acaagcagaa tcgttttgcg
     5221 aaaggtatcc agaatataaa ttatcttttc tttcagaaaa caagatgata aaagtcgagt
     5281 tttcttctca gtttttactg agtaaagttt attctatact tttagaggga acgattggtt
     5341 atattttact ggattcactt tacaaaggta cttatcaatc cttagaaaat cttagtcaaa
     5401 aaattatata agtttgcgta cgattcaaag aaaattaaga gaacttaata ctattttgaa
     5461 aaactatcat ttatcttgca gcttgaaaaa aggtaatccg atagaaggag aagaataccg
     5521 tatccgatac ttttttcact tgatgtattg gcagtcattc gacgaaaatc attctgatat
     5581 attatcaagt ataaaaaata aacgtcaact attagaaaaa tttgcgaaac atgcgccata
     5641 tacgcgtagt atagatagaa agaaatggat cagcctacta agaatttcgt tgcaacgaat
     5701 gaaacaaggc cactcgatta cgtatgtccc agaagagatg aacaaatttg tgcatccatt
     5761 gataacaaaa gaagagttta cgcattacat actagagcct ttttttgaag caaacttctg
     5821 gtcgatgaat cacatcactc caacagagca agcttacctt tattttatgt ttggtgtaat
     5881 gaacacctat ttggaagaag atttgttgga tactccagat aaagtacaag aaagttgggg
     5941 ctggattgaa tcgtctattg tagaaaattt tgaaaaaaac ttcaagattt gttttacaac
     6001 ttcagaaaaa aattatctat atgtcaattt ggcaatgatc catttgtatt caagggtttt
     6061 tgggacaaaa aagaaggtgg atgcttttgg aaaatcagca gaagacagag attttcagca
     6121 tatatttcca gtgatgtatc caatcatgca acagtttttt agtggggtag ctcgcagaat
     6181 tccagagctt aatacttatt atgaaaagaa cagacggttg atttttcaat actgtatgtt
     6241 agtcagggat atttttataa aacacgaaca acctgtcata ttctatattc aatcaaagta
     6301 tggtaaaaca caggaattat gggcaaagaa aaggatatta tctatgactg aacgcccaat
     6361 aaaatatagt gcatccgctg atcagttacc tgatattgtc attagcgatt atcctatcga
     6421 caaaaaggca tatgatcaaa cgaatacaca gatattttac tggaatggac aacctacaaa
     6481 aaatgattgg gcgcgtctga aagctgaaat cataaaaatc aggaatgaga aaaatagatc
     6541 catattggaa aaattggatt tcgttaatta agaaaagcag attgtttatg ccaaaattaa
     6601 gacattgcaa aaagattatt ttctttaaaa tcgacaagat gaccagaaag caagagttcg
     6661 ttgagctctt gctttctttt agttttagaa aggaacatcc tgattttatc ttcgctaaat
     6721 ccagtcaaaa gttttgcaga ttggtcgtag ataatcggtt ttttcaataa agaaggggat
     6781 gtacgaatct caatatatag ttcactcaaa ctgagttcat ctattctgtg aacaagattt
     6841 ttataactaa tagaatgagt agagagcatt tcggtcacgc cgttgtttgt caattttagt
     6901 aagtggataa attcattttt ggttagtggt tgacgaatta agttctgttc gataaacggt
     6961 atatcatgga cgataagcca acgttttgcg cgtcttgaag acgagcagcc agaacctgta
     7021 tacaatttaa tcatcataca ttcctccaat agctaagtat ttatgaaata ataacatgaa
     7081 gaaatagtcg aaaagtgcca tttagaggtg taaaataaga aaatgttgtc acgtataatg
     7141 gaactagcta gataagaaat actattaaat caagaaaaaa gaaagaaggc acaagtatgt
     7201 caaacacagc cgacgcaata gtagtgatcg atttacaaaa aggggtatgt tttgattctt
     7261 cgccgataca cgaattagaa acagtgacag cattagtgaa taaaagaatc aaaacatacg
     7321 aaacgattgg gaaaccagtt attttcgttc aacacgaaga tgctcaatta gtgccacaaa
     7381 caaaaccatg ggagctattg tcggaattat ctattcctga gcacagttat tttgtgagaa
     7441 aaacccatgc gaattcattt tataagacta atttgagtgt tttattaact gaattggact
     7501 gtcattcgat cgaattatgt ggggcacaag cagagtattg tatagatacg actgtgaagt
     7561 ttgctcatgg attaggatac cagcttatga tgtgtagagg agcaacgaca acttatgata
     7621 atccttttat gaaagcaaag gaaacaatcg ctttttacga gaaaatttgg gataaacgat
     7681 ttctgacatt tttggagcaa taagttgttt cttcgtttaa aaaaagcaga atggatactg
     7741 gtttatccat tctgctttat tacgtaaaat gagaaaataa tcctgttcta tttttatggg
     7801 ataaaccatt tattttttgt ataaaaaatg tttgcgttta catataattc tcctagaaac
     7861 ataaaaagga gtgaatagct tgagaacaaa acaagtatta aagggagcgt tggtcatctt
     7921 atttgctgta agtacaagcg tcccgcaatg gacagtatat ggagagagca tttcttccga
     7981 atcggaatct cgggaggtag aaaccacaga aactactgat gctggaaatg aaacgaaatc
     8041 gagtacttca caaacagaaa caaatactga aacaacggtt gagacatctg ttactagcac
     8101 agatcaagaa acgaccgcta ccacagataa ccaacaaaca gaagaagcaa cagctacaaa
     8161 aatgtcaaac gacgaatcct cttctgctaa agtaagtgac aaagagaatg ggacagaaaa
     8221 tgatgatatc ggtaaaggtg aaaatttcaa tggtaaagaa tatttgaatt acttcggtaa
     8281 acagatgaca gaaatactta atggaccaac agaagacatc aagtcaaaat taatagagtt
     8341 attagtcact gagcttgctg ttaatgatta tggtgtagat aaagagatta tcaaacatct
     8401 gattgctact tatctattgt acgatatcgc ttttgcaaaa aacagtgtgt tgatgtcgat
     8461 gcttggaggt acagatgatg ggacaacgcc tacagaaaac acaggcttct atccaaatac
     8521 aaaacaactt catcaaactg tactaaacaa acaagatggc aaaatgatgg atgtatatgc
     8581 atattatgtc gatcaaggtt cagataaaac agtcatgatc catggcggat ttcgagggaa
     8641 ttggaataac gggatcgtta cagaagaata caatgatttt tataaggccg ggtataattt
     8701 attatttgtt gattccagag cgactggtaa tagcggaggc gattatgtga cctatggaca
     8761 atatgagtca gatgacgtcc tttactggat aaatcaagaa gttcgagagc ggccatctca
     8821 aaagatttta ctttatggtg gttcgatggg agcagctacg atgatgtctg tattagcgaa
     8881 agatattcct gtcaatgtca aaggaattat tgaaaattgc ggatttgcat caattgatga
     8941 acaactacgt tttacgtaca gccagacagt agcccccgca ctaccggatg ccataaaaaa
     9001 tcagttagac atcattgggg atcaagagca tgaagattta tttatgggcc ttttgaaaca
     9061 atactatttt gatcaggaga tgcatttgga tacaaaagct gctctaccta cgattggaat
     9121 gtcagattca ttgcctaaat tgattatcca tggaacagca gatgatgtag tacctgtgtc
     9181 taacgcgcaa aaattatatg agttatcagg agggtataaa gatcttttat tagtagaagg
     9241 agcagggcat ggaaaggcac aggaagttga ccatgctgcc tacacgaaac atgtaacgga
     9301 ctttttgaaa gtagtgttcc atgatcagat aaacgtaaag tatgtggatg aaaacaataa
     9361 aagtctactc aaagaccagg acgaaatcca gctttacgga gcttatggag aaaattatat
     9421 gacagagcaa aagacattcg aaggatacga attagcaaat gttgaaggac cgactgaagg
     9481 gatttttaat gagacgatac caactattat tttctattac aaaaaaatcc cagtagctcc
     9541 accaaaaaag caagatccga cagagaatgc agacaataag ggaaagaatg aacctcgaga
     9601 ggatcaatca agtgatgata cgcaaccgat tcagatgaca gtgaaagtac aaaaaaagac
     9661 agatgcaaaa aaaagaatgt tgccgaaaac tggagaaaag aaacattcgc ttctcattta
     9721 ttcaggtgga tttttatctg ttagcacgat tatcacatgg atttggcgaa gaaagagagg
     9781 gtaaatgaat gaaatggttc aagcgaatag tgattgcagt accagttatc atcgtagtta
     9841 ttttagtcac tgtatttttg attaatcaaa tcacaccaaa gccgattagc ttgcttgtta
     9901 gaaaacaatt tgacacaagt gaaaaagaac aagtatatgc ccgaccagat gatttccagc
     9961 aaaaaatcga acaaatcgat ataaaaaaag aaatatccta tccttcaaag tacaaaaata
    10021 ataatatgga tatctacaca ccgaaagtgg aaaagaaaaa attaccgatt cttttctgga
    10081 tgcatggagg cgcatatgta ggaggggata aaaatgattg tcgtgattat ttggaatatc
    10141 tatgttcaga cacgcagcaa atcatcgtaa atatagatta tgaacgatca ccggaagcaa
    10201 aacatcctac accagttatc caattgaacg aagccattca agcagctaaa aaagagataa
    10261 aagatcaagc agattggtcg aatatttcga ttggtggaaa ttcggcaggt gcacaaatta
    10321 gtggagaata tttgttagca cttcaaaatg aagaaattaa aaaagctgat catcttgatc
    10381 cgactttgga gaacaagcag attacgaagt ttatttcttt gtctggtctg ttggatcctt
    10441 ctgcacttac tcaagtaagt gataaaatct ctagctttct ttatgaaaaa tgtggatggg
    10501 cttactttga taataaggac tttgaacaat cagagaaggt caaaagttta gccttggtca
    10561 gacatgcgga tcgttggacg caaaacacat tcttaacgga tggaaataca aatacgttta
    10621 cgaaacaaat ggaagaagta acttctgtat ttgagaaagc gggggtgaaa gtcacaaaag
    10681 tagattatgc ggagaaaaat gctgttttga accatgaata tcagtttgat ttcagcaata
    10741 aacaagcgca agaaacatat caaaagttag taatgttttt caaggagtaa agtcttatga
    10801 tgcaaaagag gttgtaatct gtcacttagc tccaagtcac aaggagcaat ttcaataaac
    10861 aagcttttca tggtttatga atattgtaac ttgtgactga ggagttagag atggcaaggg
    10921 gcacctttta ttcgaaattt gggaatatca cacgattatt gtcacgatct cttttacgta
    10981 aattttataa gtcagatttg ttgttttgat cgtttaaaaa aagcaacaaa tccgaagcct
    11041 gcggaggcaa gaagggacaa aaagccgagt ggtaagcaaa agaatgctgg ttccactaat
    11101 attccgttgt tattgatata aggctgggaa caaagatgaa tagcaaataa aatcaatgca
    11161 ctagcgaaaa aaagcaaaga aatatggata gctttcataa gcagcacctc cttttttata
    11221 attgtatgat aaatagatta agaatctata aatgtgacaa gtttgttagg atgtgaataa
    11281 aatgatcatc tagcgaactt gaaacaaaga tggctaatga taagcaatta atgattcagc
    11341 aatcagatag gtacaaagtt taccatcata cgtctagacc aataatagaa aatatgggaa
    11401 tataatagaa aagatttagg atacccccaa aagaatttcc ccaaaaattc gatgtatcct
    11461 aaactttttt attttacata acaatcatat caactgtgaa ataaaattac aaatgataac
    11521 ctaaaatgag gccaaaagcc agtgttatca aggaaatact taattcacca aaattttttt
    11581 taatttttta gaaaataaga aacaaaaata ataaaaaaaa gaaaagataa attttttact
    11641 actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc aatacaacct
    11701 tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc tgtaccaccc
    11761 ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg ttttatggat
    11821 ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg tcgaacttta
    11881 gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata tagagagtcc
    11941 aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc tgtttcttca
    12001 gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc ttggctagta
    12061 ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat tgtttctttt
    12121 gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa agaagcttcc
    12181 atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg caaaaattta
    12241 tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata gacacgttta
    12301 gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa cttttttgtg
    12361 tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag ctcagtatct
    12421 gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg gatcaacatg
    12481 gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg aaaaaaagta
    12541 gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact tactttgctt
    12601 ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc gttgtacatt
    12661 gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca taataagatt
    12721 atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa aaatcaaaca
    12781 aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga gggtataaga
    12841 aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg attgaattta
    12901 gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc aagtacagct
    12961 gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga atttctagct
    13021 acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat aaccgaaaac
    13081 atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga aggcggaaac
    13141 tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga aatcggttac
    13201 ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg cttaatgatt
    13261 ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt agcaattgaa
    13321 agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat gatccataat
    13381 ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt acaaaaaagt
    13441 gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc ggtgattcgc
    13501 ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt aagtaattat
    13561 cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc gctagaaatg
    13621 gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc acaggaagaa
    13681 gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg acatgaaata
    13741 gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt ccagcagttt
    13801 cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg ttcatttctt
    13861 tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag gattcaatat
    13921 cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca aagatgaact
    13981 gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag cagcaaaaga
    14041 acaccagaca atcgtatcta tcatacctgc tttagttgaa cgaaatgctg tagcagaaaa
    14101 taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag caagagtcaa
    14161 ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta acaaaacagt
    14221 tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc gtaacggttt
    14281 tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg aaagctttgg
    14341 tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt gtttatgtgc
    14401 gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc agatgaaaga
    14461 aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag cgatgattaa
    14521 tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag aagaaccggg
    14581 acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac atatatcggc
    14641 ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg tcgaacagat
    14701 cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata taaaaggatg
    14761 atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta tttatatgaa
    14821 gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa aagagtactg
    14881 gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt tttgaatgac
    14941 gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc tgaagccaga
    15001 cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt ggcggaggaa
    15061 aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc ggagtcatcc
    15121 ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat aaggagaatg
    15181 gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt ataacagatc
    15241 cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca gatacactag
    15301 caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat gaatcatttt
    15361 tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat cattctgcta
    15421 aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag ctttcagaga
    15481 ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca agaaatgtgg
    15541 cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat cgttttctac
    15601 atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt cgctgggaaa
    15661 aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct ctagaacaaa
    15721 tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt attgattcga
    15781 aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata cagtcaatga
    15841 tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga aacattaagt
    15901 gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg ctggttaggt
    15961 ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt cgtcgggttg
    16021 atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat gatttatatg
    16081 ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc agctatcgca
    16141 gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt tgcggtgcca
    16201 gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac ttttttaggt
    16261 aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg atcaattatt
    16321 ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat tgctttatat
    16381 ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt cgttatccac
    16441 attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac tgtatacgtt
    16501 atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag tgttgttttg
    16561 aaatctttag atattacgat ggttacttat gccatttttg ggattattat cgctttgatc
    16621 tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa caattcagca
    16681 tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa tgggaaatgg
    16741 ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa gatgtaacaa
    16801 aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa cgttacttgg
    16861 cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa gatgaagact
    16921 ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta agctggggtg
    16981 gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa gaagagtatg
    17041 aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg ggattgatgg
    17101 gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat atttttatcg
    17161 caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt ccttttatct
    17221 gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct ttcactatgg
    17281 gccgtaatgc tacaggccta aagcaaaaca ctggtgttca gacagcgatt gaaatgctat
    17341 cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa gtagaatctt
    17401 cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt gatcaaatcg
    17461 tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat acgaaaaaag
    17521 gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg ctagcaggaa
    17581 tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat ttagcgcgtg
    17641 tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa agtggagtga
    17701 attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga gatctttaca
    17761 aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag aaagctaaat
    17821 tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca aaaacagtcc
    17881 aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat attggaggga
    17941 ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat ttaaataaat
    18001 cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat atttattttc
    18061 aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag tttgatttgt
    18121 cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag aacgagctgt
    18181 ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca aaaaagagta
    18241 aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca gatgtgacag
    18301 tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga gatatcgaaa
    18361 aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta ggaagttatt
    18421 tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta ttgtccttca
    18481 ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat cctcatcacg
    18541 tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg agctactttt
    18601 tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt cgaaatactt
    18661 catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt gtagcgtatg
    18721 cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga tcgggtatga
    18781 aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg ctacagtttt
    18841 cgggaaatta tttttcaaat aaagaacagt ggctgaaaca agttgataag ttgaaagaaa
    18901 aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc tatttgacca
    18961 aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt attgctcttt
    19021 ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa atcatgatcg
    19081 ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt gtcgggacat
    19141 cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca ttcggagaga
    19201 aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta tttgttgatc
    19261 taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg actgaagaaa
    19321 agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta gaagcaatca
    19381 atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc aaacaaggaa
    19441 aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat gaggacgatt
    19501 tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga aaaaggggat
    19561 tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat tggaactttg
    19621 ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt gaaagcatct
    19681 gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca cgatgcaatc
    19741 aaggcaggga cagatgaacg agattactat attgataact atatacgaac aatcaaaaac
    19801 ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt tggatgggcg
    19861 aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata cgatcagaaa
    19921 atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca gtcaaaggga
    19981 ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct ggaaaaactg
    20041 tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt agaaaaagtg
    20101 attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga tccgccatgg
    20161 gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa aattctaaat
    20221 gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg ggcaaatccg
    20281 gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt tgtccacttc
    20341 agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc ctctacagaa
    20401 ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt cgacggtcct
    20461 atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg atatggcctg
    20521 tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat aagtaaggaa
    20581 aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata tggatggcct
    20641 tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac aaaagtatga
    20701 ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa tggcgataag
    20761 agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc gagaagcaat
    20821 caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga aaaaaggagc
    20881 agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg cttcgtctaa
    20941 tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct tgtttgatca
    21001 aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct ttttgaaagc
    21061 ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact cgattgcagg
    21121 gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc atttgatagg
    21181 gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat atgaatccaa
    21241 taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag aaagtaaagg
    21301 gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt cctgcacgac
    21361 tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct tatttgtagt
    21421 gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga tgcgtttaaa
    21481 gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt tttttaattc
    21541 ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt ctttgtcaaa
    21601 aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat ctttgttata
    21661 gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga acgcgttgat
    21721 aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt aagaaatttt
    21781 aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga atcaatcgcg
    21841 gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt atatttcatt
    21901 gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc tattttgaaa
    21961 cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa atatttattg
    22021 caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat aacggtacag
    22081 taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat ggaaatgatg
    22141 tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa gaaggccaag
    22201 cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac attgttaaag
    22261 cttaataaaa attgactttt aaccgtcttt tcgaaagaaa a
//
LOCUS       DACJGB010000051        22174 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.051, whole genome shotgun sequence.
ACCESSION   DACJGB010000051 DACJGB010000000
VERSION     DACJGB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.051"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..645
                     /locus_tag="IYI34_001951"
     CDS             <1..645
                     /locus_tag="IYI34_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR0559814.1"
                     /translation="SLKARALSFFQNGSVLTDKTLFKCKMENYHVVDQETKIALEKAK
                     SYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILFISYQELLEQIK
                     FSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTNNTLNTLLEARQ
                     NKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIKPVKGSIA"
     gene            642..1001
                     /locus_tag="IYI34_001952"
     CDS             642..1001
                     /locus_tag="IYI34_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAR0559815.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            1016..1177
                     /locus_tag="IYI34_001953"
     CDS             1016..1177
                     /locus_tag="IYI34_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAR0559816.1"
                     /translation="MTTEEVIQMRIRSIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            1180..1479
                     /locus_tag="IYI34_001954"
     CDS             1180..1479
                     /locus_tag="IYI34_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559817.1"
                     /translation="MDKKATMKRIVELTKSESWQEDKEIVAEVQRIGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            1479..1796
                     /locus_tag="IYI34_001955"
     CDS             1479..1796
                     /locus_tag="IYI34_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAR0559818.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILDEYME
                     GSK"
     gene            1793..2179
                     /locus_tag="IYI34_001956"
     CDS             1793..2179
                     /locus_tag="IYI34_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559819.1"
                     /translation="MIPRFRSWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFEGDVVLVNVSNGFDHLVNEKTVVQESEFHSGLVCKSLISGMEYRI
                     FSHQKMGYEYEVTGNIHENSELLEVE"
     gene            2176..2511
                     /locus_tag="IYI34_001957"
     CDS             2176..2511
                     /locus_tag="IYI34_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559820.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDIHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEQ"
     gene            2657..3226
                     /locus_tag="IYI34_001958"
     CDS             2657..3226
                     /locus_tag="IYI34_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAR0559821.1"
                     /translation="MNKQEAIKKLESIKVIGNDALAACYNESINAGITLMKKIDEPKK
                     LVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLYSVIIAGEYLVKEISCSNEVKLVSTAYLPQYLAH
                     HYQLTEKQIKAIDERYWPFAVPVEEVAEG"
     gene            3393..3698
                     /locus_tag="IYI34_001959"
     CDS             3393..3698
                     /locus_tag="IYI34_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559822.1"
                     /translation="MNVQVYLKRDLARFPRSWDTNSHPSEAEEEGYLMSANMIEITRD
                     DADEFDKKRSWYSVPNPMYTAVIEDYHISDRFILIDLERPKKRIKQYRRCGFSTKKW"
     gene            3722..3919
                     /locus_tag="IYI34_001960"
     CDS             3722..3919
                     /locus_tag="IYI34_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAR0559823.1"
                     /translation="MNNRHRRVAKLRNQELNVAKVKFEREYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILDIKWEDTE"
     gene            3916..4122
                     /locus_tag="IYI34_001961"
     CDS             3916..4122
                     /locus_tag="IYI34_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559824.1"
                     /translation="MKIYVVKFGNQFYRSDERSIGANTLSIVDILQSARWFDNLEEAN
                     QVSRRLGGLTQVYELVTVDHEGVE"
     gene            4122..4418
                     /locus_tag="IYI34_001962"
     CDS             4122..4418
                     /locus_tag="IYI34_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559825.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFRGYVEKVLSNSAIIRIENTMECDKWLAKSKENLAVARLVDMEVIESK"
     gene            4494..4907
                     /locus_tag="IYI34_001963"
     CDS             4494..4907
                     /locus_tag="IYI34_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAR0559826.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5784..6128
                     /locus_tag="IYI34_001964"
     CDS             5784..6128
                     /locus_tag="IYI34_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAR0559827.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            6133..6414
                     /locus_tag="IYI34_001965"
     CDS             6133..6414
                     /locus_tag="IYI34_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559828.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6517..6831
                     /locus_tag="IYI34_001966"
     CDS             6517..6831
                     /locus_tag="IYI34_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAR0559829.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6809..8503
                     /locus_tag="IYI34_001967"
     CDS             6809..8503
                     /locus_tag="IYI34_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAR0559830.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8523..9701
                     /locus_tag="IYI34_001968"
     CDS             8523..9701
                     /locus_tag="IYI34_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAR0559831.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9664..10350
                     /locus_tag="IYI34_001969"
     CDS             9664..10350
                     /locus_tag="IYI34_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAR0559832.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10350..11510
                     /locus_tag="IYI34_001970"
     CDS             10350..11510
                     /locus_tag="IYI34_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAR0559833.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNDFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11520..12395
                     /locus_tag="IYI34_001971"
     CDS             11520..12395
                     /locus_tag="IYI34_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559834.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12392..12703
                     /locus_tag="IYI34_001972"
     CDS             12392..12703
                     /locus_tag="IYI34_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559835.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12693..13046
                     /locus_tag="IYI34_001973"
     CDS             12693..13046
                     /locus_tag="IYI34_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAR0559836.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            13036..13437
                     /locus_tag="IYI34_001974"
     CDS             13036..13437
                     /locus_tag="IYI34_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559837.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13430..13834
                     /locus_tag="IYI34_001975"
     CDS             13430..13834
                     /locus_tag="IYI34_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559838.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13846..14451
                     /locus_tag="IYI34_001976"
     CDS             13846..14451
                     /locus_tag="IYI34_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR0559839.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTNTATQSQ"
     gene            14471..14833
                     /locus_tag="IYI34_001977"
     CDS             14471..14833
                     /locus_tag="IYI34_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559840.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14836..15018
                     /locus_tag="IYI34_001978"
     CDS             14836..15018
                     /locus_tag="IYI34_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559841.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            15035..18454
                     /locus_tag="IYI34_001979"
     CDS             15035..18454
                     /locus_tag="IYI34_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAR0559842.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18505..19242
                     /locus_tag="IYI34_001980"
     CDS             18505..19242
                     /locus_tag="IYI34_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAR0559843.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            19252..21543
                     /locus_tag="IYI34_001981"
     CDS             19252..21543
                     /locus_tag="IYI34_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559844.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21567..>22174
                     /locus_tag="IYI34_001982"
     CDS             21567..>22174
                     /locus_tag="IYI34_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559845.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVIFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYS"
BASE COUNT         7904 a         3252 c         4779 g         6239 t
ORIGIN      
        1 agcttaaaag cacgagcgtt gagttttttt caaaatggtt cggtattaac agataaaact
       61 ttgtttaaat gcaaaatgga aaattatcac gtagtggatc aagaaacaaa aatcgcttta
      121 gaaaaagcaa aaagctatac gaatgaggtt ctgctgaacc atcctgcaca tttcattcta
      181 tcagggaaat caggaagcgg aaaaagccac ttatcaatgg ccacagcttg ggaaatactt
      241 gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagaatt attagagcaa
      301 ataaagtttt cttataacaa tactgaactg agaaaagaaa ttgaaggatc gcttatagcc
      361 gatattaaaa caactgattt ggtggttttt gacgatattg gagctgaatt aggtagtggg
      421 gtatcaaata gtaggcagtt tacaaacaac acgttaaaca cgctcttaga agccagacag
      481 aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga agcctacggt
      541 gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa attccaacaa
      601 acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa taccgtaata
      661 aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag tattacgatc
      721 tagccttgtg gcaagctgaa gcaaatggct ggaaagtaaa acttcaggaa agatttgagc
      781 tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat attcccgact
      841 tcacatttta taaaaacggc aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa
      901 aagactttaa gatcaaggca aagttgttct gtcatcaata tcaagcgccg ttgatattag
      961 ctaaaaaata tcggaatacg ttcaaggaag agcgttttta acgaggtggt ccatcatgac
     1021 aacagaagaa gtgattcaaa tgcgaattcg aagcattcaa cgtgaaattg acgatctaga
     1081 acgaacaaag gcagtgatgg tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga
     1141 gaatttaaga agggaaattc gtagattgga ggaataaacg tggataagaa agcaacaatg
     1201 aaacgaattg ttgaattaac caagtcagaa tcttggcaag aagacaaaga aatagttgca
     1261 gaagtccaaa ggatcggcaa atcaatgtgg actgaaaagc ccaaacggag aacgccaaga
     1321 aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga acagttatct
     1381 gaaattactg gattaagcaa aaacatcatc tgggatagag ctaggagctt atggattgat
     1441 tcaaaaggac gacagtttag gtatgtggag gagaaataat ggatctcatt acacaatata
     1501 gtgatatcat cctcaagaaa atcatgatga agattcagaa agacaaaaaa tcaaaagaac
     1561 gagcggaatt agttaagttg gaaatggctg aaacaggagc aggagtgcga agtagcaggc
     1621 attggaaagc agcagcaaac attgaatttt attacaacga aattcaaaaa gggttcgatc
     1681 agatgcgtga gctggatcgg caaacaaatt ggagcaagaa acttcatcaa gatcgtttca
     1741 aatttgtaga gaaatataaa gagatattag acgaatacat ggagggcagc aaatgatacc
     1801 gagatttcga tcgtggtaca caccatttaa aggtaaaaca attggacaag aaatgaaata
     1861 tgggcaagca ggaaggttga tcactcatgc tgaaatggct ccagataaat atgtgcttat
     1921 gcaatccaca ggactgaaag ataagaatgg cgtagaaata tttgaagggg atgtagttct
     1981 ggttaatgta agtaatggtt ttgatcattt agttaatgaa aaaacggttg tacaagaatc
     2041 cgagtttcat tccggattag tttgtaaatc attaattagt ggaatggaat atagaatatt
     2101 cagccaccaa aagatgggat acgaatatga agtcaccgga aatatacacg agaatagcga
     2161 gttattggag gtagagtgat tatcttagct actgactaca tcgattcttt aaaagatgaa
     2221 aatgggaatg tcccagcatt gctaaattgt ggaattaaaa gtaagaatag aatcattttg
     2281 ttacggatat cggctgattt agcaaaatat ctttatctga gcaagacagc agtatattac
     2341 actgcggcta cacgttatca gtacaaggga aaaacaatca gtcaggatta ttatgatcgt
     2401 ttaatcagtc ctgacataca tggaaaatca aaatcagcga tcaagttatt tggcgcaata
     2461 gagatatatg ccgatgattt tccaaattta tggttgaagg aggaacagta atgaaagact
     2521 ggttagaaga agcaagcgcc ttagttggct gtttcataaa ctgtgcgttt atatctgtaa
     2581 ttattgcact ggtgggattt gtatcattga aagtgattat attgctttgg cagttaattt
     2641 tttaggagga acagcgatga ataaacagga agcaatcaaa aagttagaaa gtattaaagt
     2701 gataggaaat gatgcactag ctgcttgcta taacgagagt ataaatgcag gtattacgtt
     2761 aatgaaaaaa atagacgaac cgaagaagct agttattcca caactcgtgg ccggttggct
     2821 tgagaaatct acggaccctt ttacgaaagc tgaaaaaata gcgtatttaa tcaaatctaa
     2881 agatggtgat tcatattatt tctgtgattg gtttgtacga gatggcatag tgacgcaaga
     2941 acaaggagaa gaattactcg cttgggcaac gagacaatca tatgaaacac tattgagctt
     3001 gtacaacggc tacgaggtcg agaaagagcc gttgtacagt gtaataattg ctggcgagta
     3061 ccttgtcaag gagatcagtt gcagtaatga agttaaacta gtatcaacag cttacctgcc
     3121 acaatacctt gcacaccact accaactgac cgaaaaacag atcaaagcaa ttgatgaaag
     3181 atactggcca tttgctgtgc cagtggaaga ggtggcggaa ggatgagtag acagataatg
     3241 ctccatatac cagacggaac aaaggcaatg acgattacca ttgttgctgc acaaaaagac
     3301 ggtgggttgg cgttgaaaac aaaaagcatt gataccaaac agattttaga aggcagagat
     3361 gtcgagatag agatcgatga ggaggaagcg gaatgaatgt tcaagtttat ttaaaaagag
     3421 atttggccag gtttcctcga agctgggata caaattccca cccatccgag gcagaagaag
     3481 agggctatct aatgtctgct aacatgatag aaattacaag agatgatgcg gatgagtttg
     3541 ataaaaaaag atcatggtac tcggttccga atccaatgta tacggctgta atagaagatt
     3601 atcatatatc cgacagattt attctgattg atctagaaag gcctaagaaa cgaattaaac
     3661 agtatagacg ttgtggattt tcaacaaaga aatggtgata acaaaacaga ggtgaagaaa
     3721 aatgaacaac agacaccgca gagtagcaaa actaagaaat caagaactga atgtagcgaa
     3781 agtaaagttc gaacgggagt atggtgtttc tgcagaagaa acatataaag tggcaagtca
     3841 gtgtgttgct gatgcaagtg atgctattcg taagtttgga atttcgatac tagatattaa
     3901 atgggaggat acagaatgaa gatttatgta gtaaagtttg gaaatcaatt ttacagaagc
     3961 gatgaacgtt ctataggggc taacacatta tccattgtag acatactcca aagcgcaaga
     4021 tggtttgata atctcgaaga agctaaccaa gtttcacgac gacttggtgg attaacgcaa
     4081 gtatatgaac tggtcactgt cgatcatgag ggggtagagt aatgaaacta aaagacggat
     4141 tttacgctag tagtcatggt atcggcggtt tgatgctaga tatgccgaca aagaacccta
     4201 aaacacgtga gaaaccaaaa ttcaaagtcg gtgacatggt tcgctgtgaa gcagaaggat
     4261 ttatctatcc atttcgtgga tatgtagaaa aggtattgtc gaactcagca atcattcgta
     4321 ttgaaaacac gatggaatgt gacaagtggt tagcgaaaag caaagagaat ttagctgtag
     4381 cgagattagt tgacatggaa gtaatcgaga gcaaataaaa aagccggatc gctccgacta
     4441 acataataaa acagacaagt ttattatatc acataaagga gcggtttgac ttgatgcaat
     4501 tgttacgaga ggtagatttc aaacagacaa gatgtaatgc gagagatgtg ctgaagaact
     4561 ttcggcgttt ggagcggatg gcaggtcgct ctttgataga tattaagtct ccaattatta
     4621 cggatatgcc gaaggcgccg aagcacggca ataaggcaga ggacgcgatc attcagatga
     4681 tggatataga agcagagaga gatgcgattt tagcggcttt gatggctctt agtctgatta
     4741 gccgtcagat actctactac agcttttgtg tgccagatag cttctcaaac tacagaatta
     4801 gccgtgaagt gggttattca gaaagaagta tacaacggat gaagtcggaa gctctaatag
     4861 agtttgcaga agcatataaa cacggaagaa taattgctta taaataattt ggcggttttt
     4921 tggcggaatg atggcggttt ttagctattt accagtgata ttatggtagt gtcgaaagat
     4981 tagtgatagg tctgagacaa aataataata aaaggaacat cgttttatta ttgtttcaca
     5041 attaagcttc gatagacagc aacggaaata ttaagaataa ggatgtgaat ttcaactcct
     5101 tctaaattgt tcttattatc tatcatccgt tgctgtctat tgttttttta attattcaca
     5161 cgataactaa aggtgagtga agagaaatga ttccattaat aatttcaatt tttgcgctct
     5221 gtcttaatgt ctatatgatt ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa
     5281 gaataatttt atagtgtcac tgtggcggaa agggtagacg cttaaaaata aggtcaatac
     5341 gtcgagggat agccttaacg ttttatgatt tgaccatgca atgttcgatt cattgccagt
     5401 gactttggca accgagggtt ggaaatgggc gctcaaagta cacgagcaag gcgaggtcga
     5461 tagtaatcga tggaatcggt gtaggttgct tgatagagct ataactgcat ctcgtttatg
     5521 aggtgtagtt tttacagatt agatcactct ttgagtggtc tttttatttt acacaaagga
     5581 ggtaacaaga atgtatagac cacaatactt agaacagaag tatgaagtaa tcactgtgca
     5641 aaacggtaac ggtgagatag tacgaaagta tagaagacca ataaagagcg atacatacaa
     5701 acgaaaggaa agcaatgaag ttattccatt gtatggcaaa agaatagcta agcattaaat
     5761 aagattgcga aaggagacgg aatatgaccg aggaattcta tagatggcta ttacagttga
     5821 taagagaaga tcgtttagtt aagttctatc agtctcctaa atggcgcagg cttagagaga
     5881 aagcgatgaa acgagatcac tatgaatgcc aagagtgtag aagactaggt aagtatcata
     5941 gagtagaaaa cgttcatcat ataaaggaag tcaaggatag acctgactta gctttagatt
     6001 tagataatct tatttgttta tgtgttgaac atcataatga agttcatggc agatatctta
     6061 cagcattaga taaacaagag aagaagatag aaagctttgc taacttcgat gcaagtgaaa
     6121 ggtggtaagt gcatgatcat caatgataat ggcagagagt atgatacaga aaagattgaa
     6181 gagtattcat cttatactca gggattaatt aaacgtttga tatacgttcg ctatgtaggt
     6241 attagggatc tgttatcaga taactgttgt agtaaataca aagtgaatca agtaagagaa
     6301 gcgttgaata aagataataa cgtcgaaaga ataaaaaatg tttttggata tagtattgaa
     6361 gagattaatt attacattga cttcgctgaa gctttcattc cgatggtgag ataacccccc
     6421 cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg agggggctgt caggaaaaga
     6481 gattttttcg aactttatca tgaaaggagg gctaaaatgt ttaaaaacga attgtctcaa
     6541 aatcgctaca gagaaaaatt acgccgctct ttaataagcc aattggaaag tcagaaaaca
     6601 aatattgagc cattcttaga taatgttgat cgttatatca gtttatggga aacggcgata
     6661 tcactggaag aagatatatc cgagaacggc attagactgg agaatggtaa aaagaatgaa
     6721 tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat tgatgttgga taaacttgcc
     6781 attactcctg aattggtagg tgaagcaaat gaatcaattc ctgagttata agcatattga
     6841 aaattggttc aaagctatag aagaaggcac tatcaaggta tgcaaagagc aattattgct
     6901 aaaaaattat ctagaagaaa gagtctttac tagagaagat atttacttcg ataagcagat
     6961 ggtagaggat tcaatcaata taccagcaca atactttcca ttcgaattaa ttccgtggga
     7021 aaaatttcta caatgtttta tttatggtgt tcgatggaaa aaagataaaa cactagtgtt
     7081 caatagatat ctttcattaa tgggacgtgg taatggtaaa actggttttg cttcttggaa
     7141 caacttcttt ctactaaccg ctaaacacgg tattaaaaat tatgatattg atatctatgc
     7201 caataatgaa agccaagcaa agactagttt tgatgatgta tttaaagtaa ttaaagatca
     7261 tcctgattta gataaaaaag tatttaaagc tacgaaggaa gttattcaaa atatcgctac
     7321 aaacagcaaa cttcgttata acacggcaaa tgctagaaca aaagatggga agcgaccagg
     7381 tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat tcaatgataa atgtggctac
     7441 ttctggtggt ggtaaaattc gagattatag agaattttat gatacaacta atggtcatgt
     7501 tcgtggtggt ccgcttgatg acattataga agaatcaaaa atgattcttt ctggagaact
     7561 tggaattgac aaggatggag cagaattttc tagtttgttt ccatttattt gtcgcttgga
     7621 taacgataat gaagttgatg atcccgacat gtgggaaaaa gcttgtccaa ctattaatta
     7681 caatgcagat ttaaaacgga aaatgtttca agaatactct caaatgcaac gtaatgctgg
     7741 tttaagactt acgttcatga ccaaacgaat gaacagacct atggaagata cacgatttgc
     7801 tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa gaatttcctg aaaaaatgga
     7861 tgaagtgata ggaacagtcg attttgctga tagacgagat tttgccagcg ttgggttgct
     7921 aggaaaatac gataaagatg tttattttac acaacatact tttatccacg aatcagccct
     7981 tcgattacaa aacatcaaac gagaggttat agatatttct atagatcaag gaaaatcaca
     8041 gatcgttcat ggaaaaaata tagaagctga ttatattgta ggttggtttc ttgaaatgag
     8101 taataaatat tatattaaaa aaatcgctat ggatatgtac cgtgcaaaaa tattgaagcc
     8161 cgctttagaa gaagcaggtt ttactgtgga aattgttcga agcggatctg ttacacatgg
     8221 tatgttaaaa gatctggttg atgacctttt tattaatcaa cgtttatttt ttggtgacga
     8281 tgcgattatg cgttggtatt gcatgaatgt atatgaagag catatttcta atggaaatat
     8341 acgctatgaa aaaatagaac ctgaaactag aaaaacggat ggcttttttt cattccttca
     8401 tggtttgaat tttttagatg atatttatga ttctgctcct gtaacagtca caaatagctc
     8461 agtagaaaat acaggaactg gatttactcc tctagtattc taacttgaaa ggaggtgaga
     8521 aagtggggat ttttcaaaag gcggtaggat acttcacaaa aaaagcaacg gttcctttag
     8581 aagaatactt ttgtaaattg caagttgatt ttgtgtatcg aaaatttgcg attgaaactt
     8641 gtattgattt gattgcaaat gcgatgagta aagcggaatt caagtcatat gaagatggaa
     8701 aaaataaaaa gaatgatctt tactataggc tgaatgtagc tcctaataag aaaaataatg
     8761 caacagaatt tagaaaaaaa ctgatcagga gattaatatt ctacaatgaa gtattgatcg
     8821 tttctccgtc taataattct agcgaaatat ttattgcgga tagttgggat gtcacagaat
     8881 atgcattgaa agatgatgtg ttttctcaag tgcaaattaa caacatagtc cttgatagag
     8941 aatttctaga aagtgatgtt atctatataa aatacgcaga tcaacaaatt aggcaactag
     9001 tcgatgcgta ttatcaagcg tatgggaaac tcatttctag tgccatgaat gtttacaagc
     9061 gctctaacgc tcgtagatac gtactgaaag ggaatttatt ccgaccgcaa gacaatacaa
     9121 cacaagatca aatcaataaa atgatgacat cacaatttaa ggcttttatg gaagctgata
     9181 atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt agaagatttc agcggaaact
     9241 tccaaagcaa ttcaagagat ataaaaaact taatagacga catctttgag atgacagcag
     9301 cagcgtttca cgttccgaaa aacctactaa agggagacat gagtgggtta tcggatcaag
     9361 tggacgcttt tttaatgttc gaaatcatac cgattgctga acttattcag gatgcgttta
     9421 acgctagtct ctatgaagta gaagaatact tgtcagggaa ttttgtacgt gtggatacaa
     9481 ctatgatcaa gattactagc ttcaaagatt tggttgacgc tattgatgta ggcattagaa
     9541 atggagtatt tacaatcaac gaaggaagag agcgcgttgg aaatgatcgc tctgataagg
     9601 cgatggcaga tgaaatattt ataactaaaa acaatcaaca agtatcgaaa ggaggtgagg
     9661 cgaatgacga caatgaaaac atttctagca gtaaagaatg aaggcgcagt accgcaaatt
     9721 tttattcagg gatttattgg ttctagttgg ttctttgaag ggaatactga caagggaatc
     9781 aaaaatattt tggatagtct aggtgatcaa gaagaaattg aagtagtaat taattcaaac
     9841 ggtggagacg tatttcaagg gattgctatt gggaacttac ttaagtcaaa taaagcaaaa
     9901 gttaacgttg tgattaacgg cttagccgct agtgctgctt caattatcgc aatggctggc
     9961 gatactataa aaatctacaa caatgcacaa ttgatgattc accgcgcttc cacatacgga
    10021 gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac tggaatcaat tgataaatcg
    10081 gtaaaggctt catataaaac acgattcaat ggcacagatg aagcattgca agaacttctt
    10141 gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt atggattggt cgatgaaatt
    10201 atcgatgcag aaaatagctc aggtactgaa gctaaaaaag aacaaagcgt tgaagaaatt
    10261 ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg catttacagc agcattaaat
    10321 aaaacatttg gacaaggaga tgtaaaataa tgacagttaa aaatttaaaa ggtgtaacag
    10381 ctgcaagcga ccaattgatg aaagctttta aagatggtaa cgaagaatct tttagcgcag
    10441 ctatggtaag cttatctaag gaaattcagg ataaaatttt agaagaagca acagcaaaaa
    10501 atcaagatca attagtatta atgaaccgtg gtcagcgtgt attaactaca caagaaacaa
    10561 aattctataa cgaagtagtg aaaaacgaag gttttgcagg ggtcgaagaa ttagtgccag
    10621 ctactgtatt tgaacgtgta tttgaagatt tagaacaatc tcatccacta ttgcaaaaaa
    10681 ttacttttgt taacacaact ggtgtaacag aatggattgt gtcacgtgga gtcaatccag
    10741 catggtgggg taaactttgc gaagctgtta aaaaagtttt agataatgac tttgatgtaa
    10801 ttaacatgaa gcagttcaag ctatcaggtt atattcctgt atgtaaggca atgcttgatt
    10861 taggtccagt atggttagat cgttatgtcc gtactgtttt agtagaatca ttgagaattg
    10921 cattagaaca agcaattgtt gatggtactg gtaaagatat gccagtcgga atgatgcgtg
    10981 acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa agcagaacct attacagctt
    11041 tagatgctgt aactatgggc ggtttgatgg cgcgactatc aaaattcaat atcgaaggtg
    11101 tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt ggtcctaatt gtgaatccaa
    11161 ctgattactg gtctaaagta ttcccagcta agactgtact aactgctaat ggagaatacg
    11221 tacaagtatt gccagtacca gtttcagatt tgcagtcaac ggctgtgcca gaaggaaaag
    11281 cagttattgg ggtagcctca gattacttca tgggtgtagg atctacacta aaaattgaag
    11341 cttcagatga ataccatttt gttgaagacg aacgcattta tctagctaaa caatatgcaa
    11401 acggtcaacc taaacgtaac gatagtttca ttgtattaga tattagcgct ttgggaacta
    11461 ctactacaac tacaaaacca acaaccacaa caactacaac acaagcgtag gtgatcagaa
    11521 tgaagtatat tctttgtcag ccggcaatca atcggtttaa atgggagctt gaagtttgtt
    11581 taactaatct gaagaaacta ggaatcaaag atatcgtatt gcttttcagc agacacgatg
    11641 atcagattcc tatttttttt gagaaggaat atggtgttga agttcatgtg tacgatgatc
    11701 tgcgggacga caaagagtat attccttcga ttaaaccata tttatggtgg aaatatttag
    11761 aagaagatca ttcgcgtgag gacgaccgat atttctatat cgattcggat gtcattttca
    11821 ataaaagaat taatttgcgc aaattgcctt ctaaagatga tgtttggtat tgtagcgact
    11881 gctgtagtta tctaagtctt gattatatta gaagctgtga aaacggagaa aatattctaa
    11941 aagatatggc aaacattgta aatgttacag tagaatcttt ggaaactata aacactaatt
    12001 caggaggcgc acagtgggtt attaaccgcc ctaaagcgaa ttattggaaa aaggtttatc
    12061 tggattctaa tcggctatat cgctacctta gagggcaaaa aacaaatata aaaatctgga
    12121 cagccgagat gtgggcacag ctttggaaca tgatgtattt caatattggt cctaaagttc
    12181 acgaggaatt agacttttgt tttgctactg atccaataga aaaagttaaa gaagtaaaaa
    12241 tcttacacaa tgctggagta acaacaaacg atgaagattt atttttcaaa gggagatacg
    12301 tgacttctac gccttttgat gaagatttat catttgtaaa caagaaaaaa tgctcttacg
    12361 catatgttaa agcaattaag gcggtggtta gatgacgcct gaacaagtga ctgaagaatt
    12421 gctaatagct gtgaaggata atatttacgt tacctggaac gaagaagatg agtcaattaa
    12481 aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa gtgagtacaa cactttcttt
    12541 ttctcctgaa agcttagaat acggattgct aatcgaaaga tgtagatacg actggaatcg
    12601 tgctttagat gagtttgaac aaaatttcgc tagtgagtta ttaggtttca ttcaacatta
    12661 tgcgctacaa gaatatattg caggtgatgt gaatggcgaa taatcgtaga ctcgaagaaa
    12721 cattcaacga tggttggtta aagattttga cgcaaactac caaaagaaat gaactaggaa
    12781 aaaagattgg tgtagaagat acagaaatca cttctttaaa atttagaaat ctttccatga
    12841 gagatagtga tataacagct atggatgcga tgggatcgaa attaactaag aaagtaaaga
    12901 ctccatttca tccaatcgcc aagaaattta ataaagatca atattttatc gtaatcgata
    12961 gtatgcgtta caacgttatc tatgccgatt acgataattt ttatatctat ttttatcttg
    13021 aaagtgtggg tgaatatggt gattgataat tctaaagaaa aagaacgttt aaataagcaa
    13081 atttctgcta tcaaaacttc cttagaagag cattttggcc tcaaactctt tcaagactcc
    13141 gtaggcgagg atgagctacc tgatgatttt aattacttca ttctcgaaac aggagaaata
    13201 gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc tatatctaac tttctattca
    13261 gaaaatagag aagatttaac aggagactca ctagatatta tttcattgat tcaaaatcgt
    13321 tcgattcgtt ttcagagaat ggatcccaat catttaaaac tagagaacca agatcgctat
    13381 atcgatcaat tggtatttac gtttagacga ttattgaaga gtgattgtca tggctaaaaa
    13441 tagttgggag ctaaaaataa atggacatga tgaacttctt gtgcggatgg aacgctattc
    13501 aagcgagagc gaacgactga ttaacgaagc attgaaatca aaaggttcgg ctattgcagt
    13561 ggataggatt acagaaaaaa ttcctgtttc tgaagcagat ttaagaagag ggcaccaaca
    13621 cgcaaaaaat agtcgtccac ttaagactca atacattaat ttgggtttca tcattagacc
    13681 tacaagaaaa tttgagtatt taaaatatcc tgatttgggg ataggtactt ctaaaagaaa
    13741 tcagccggac gaatttatga gaagaggatt aggtcttgca cttgatccaa ttacagaact
    13801 tctgattcgt caattcgata aattaaataa ataggaggaa caacaatggc taaaacaaca
    13861 acagtaacaa cttttgacaa tataagtatt aaaagaattt cttttaattt taagaatgca
    13921 acaaatgcaa tttcaactga ttgtaatgga caattagatg gcgaaacaga aatgcagaga
    13981 attgtaaaaa aatgcggttc aacagaagta aaatcgaaat ctaaaccaat caatatgacg
    14041 gtaacaatta ctgcacatgt accgatggaa gtttatcgac gcttcaatgg gttgaaacaa
    14101 gatgaacgta ttaaatcggg aatttactct tatggtcctg attccgtagg ggaagatttc
    14161 tctcttgctg cagagatcgt ggatgatttc gaagaaaaaa ataagctgat tggtatgtta
    14221 gcatgcactt cgaatacagg attaacattc tctattgaaa atggtgcgga tgaagtagct
    14281 gcgttagaac tagaaacaaa agttatgcaa gatgaatttg gtaaatttta tcatgaagca
    14341 attgttgcag aacttgaaga agacttaaca gatcaatgga tgacgaatct atctgctgat
    14401 gtgattaaaa aaacttcagt tgtgacaaat acggccactc aatcacagta aaaaaaacgg
    14461 aggtagcgaa atgaacgaag attactcaaa aattgaacta aacgatggaa caattttgaa
    14521 tttagaacct aaactgaata tcaagaaatt attgatgatc aatagagatt ttaacacaga
    14581 cgagtttgca aaaatgactg tgggaaaagg atccatggat atttctgtta ttcaaggtgc
    14641 aaaggctgtg tatattgctt accgccaagc gaacatgact gattatattt cattcgatga
    14701 atttatcgat aaatgggatt ttgatatggc tactgccagc tatatttatc aattgatgat
    14761 gttcaaacaa gcacgcgatg cttatcaaaa agaatttgaa aaagcaaata aggaaaaaaa
    14821 gcttcaaaag taaaaatgcc aaagctctta gttgaaacgt gggtcgatgt ctattcgatg
    14881 ttgaccgacg ttttttctat gccttcagat ttggttttaa gcgatatctg tttagatgac
    14941 attttgcaaa tggcttacaa caagagtgct tatgaaggat ggaaaaacta tgcaataaac
    15001 caatcccaga aaaactaaag aaaggaggta aaaaatggct aaaaagagaa cagaagcaga
    15061 agtaacattc atagctaacg atgacggatt gaaatctacg ttaaaagaaa tcagtgctga
    15121 attaactaaa aatagagcag aattaaaact agaacaagct caattacaac agactggttc
    15181 tgaatcagac aagttaggaa gtaaattatc ttctttagaa aagcagtatg aattacaaag
    15241 tcaaaaagtt gaagtaacta gccaacgttt agccaatgcc aaaaaatatt atggagaaaa
    15301 ttccaccgaa gttcagaaac ttgagagaga actgattaac caacaaacag cgcaacaacg
    15361 tttgtcaaac gaaattgata aaacgagtaa tgcactagct caagcaaaag gcgaaataca
    15421 gacgtacgag tctacaatgc aacagttgga tagtgaacaa aaaaatgttc aagctagtgc
    15481 ttctctgatt gaatccgaat acaaaaaatg gcaagcaact gctggtcaat cagcttctga
    15541 atccgagaaa ttagcgaaag cccaagaata tgtttctcaa caatctgaaa atgcagagaa
    15601 aacgatagat atcctgagac gacagttaga agctacacag tctgagtttg gcgctacatc
    15661 cacagaagca atgcagatgg aggcgaagct taatgatgct gaacgtgaat ttgaagagtt
    15721 aggacaagct gctaaaaatg tagatacaac taacttggac gatatcggaa gcaaaataga
    15781 tatgaataat ctaatggaag cttctgacgt tttaagcgac attggcgata agcttacaga
    15841 attagggaaa caagcagtgg actctgctaa tagtgtaggt agttcccaga gtaaaataca
    15901 agctaatttt ggtttgacta aacaagaggc tgaagaatta acgaatgtag ccagagacat
    15961 ttattataaa ggttttggag aatcgttaga tcagtccaca gatgcattga ttttggtaaa
    16021 gcgtaattta ggcgatttaa ataatcaaga tttacaaaat atcacggaac aagctatggt
    16081 cctagaaaac accatgggcg ctgatatgga tgaaacgtta cgtggtgtaa atggcttaat
    16141 ggtcaatttc ggcttgagcg ctcaagatgc aatggattta atggtttcgg gtactcaaaa
    16201 cggtttagat aaaacgcacg aattaggcga caatatggca gaatatagcc aattatggag
    16261 tcaaatggga tattcagctg atgaaacgtt cggaatgctt caaaatggtt tagatgcggg
    16321 tgcttataac cttgataaag tcaatgactt agttaaggaa atgggaatat cgttaacaga
    16381 tggtcgattt gagcaaaaca tggatatgtt tagtgaaagt actagaaaag cttttgaaga
    16441 gtggaaaaat ggcggaggaa cacaaaaaga cgttattaat tccatgattc aagattttag
    16501 caatatggat ggtcaatacg accaattaaa taaagcttct acaatttggt ctgcacttgg
    16561 cgaagataac gcgatgaaag ttgtccaatc tttgactgat gttaaccata catttgatga
    16621 tgttagtgga tctgcacaaa aaatgaatga agattctact actccgttac aagaattaaa
    16681 cggaaaaata gctgaattaa aggattcatt agctcctata ggcaacacaa tcatagatgc
    16741 actcgaacca gtaattgatt ttctaggaaa gatggctgat gcgtttaata atcttccaca
    16801 accagtacag gattatgccg tagcgattgg cggattgact gctgcattta ctttattaat
    16861 gccaataata gttggcttca tggctctagg tggtcctact acattaataa taggagcagt
    16921 tattactgtt attgctggag ttatagcaat tataaaaaac tggggcgcaa ttactgactg
    16981 gtttaaggga atatggagta aattcactga ttggttgggt ggtacttggg aaagtataaa
    17041 agaaggtgcc tcatcagttt gggatggagt taaagaaacc tggtctggat ttgtagattg
    17101 ggttcaagat atttggcaag gagtttctga ttggtttgga gagctatgga gcggattagt
    17161 tgaaggagct tccaacatct ggcaaggagt ccaagagact tggcaagcat tcgttgattg
    17221 ggtttcaaat atttggaacg gagtcaaaga agtatggtcg attatttggg cagacattgt
    17281 aggaattgtt caaataccat ggaccttaat aacgtcattg attcaagccg gtattaatat
    17341 tatcgtgggt atttttgatg tagctggaca gttattaggc gcagcttggc aagctgtttg
    17401 gacacctatt tctgatttcc ttaaaaacac ttgggatact atgacacaat gggtaagcat
    17461 cgcttggaat ggaattgtaa ctacattcca tactatattt gatccagtag tggcatggtg
    17521 gaatggtata tggacagcta ttagtactac ggcttcaaat atttggaatt caattagtgc
    17581 aacagcttct agtatttgga acagtatcaa gaatacaatc actagcttgg tacaagcagc
    17641 tgctacagta attcaaaata tttggtcaac tgtatctagt tggttaggtg gaatttggaa
    17701 ttcaatcagc tctacagcat caaatatctg gaatagcgtg actagtagta taagcaatgc
    17761 tataaacgca gctaaaagtg ccattcaaag tgtttggaat agtatatctt cgtggatcag
    17821 cggaatttgg aacggtatca aaaacactgc tttgaatctt tggaatggaa ttacaagcac
    17881 tattagctct aaagtaaacg atggaaaaaa tgcaatttca agcggttggt ccaatctaac
    17941 aggtattgtt tccgacatat tcaataatgt taaaagtaca attgctaaca tttgggaagg
    18001 tatcaaaaag actgttagcg ctccaattga ttggatcaga gataaaatca gtagtatctt
    18061 tgataatttg aatatttcta taccacatat tccgttacca cattttaaat tgagcggaga
    18121 attcaatcca ttgaagggga aaatcccaac gttgggtgtt gattggtatg cgaaaggtag
    18181 tgtgtttaat tctccgaata ttatcggtgt cggcgaagca ggacctgaag cagttttacc
    18241 tttgaaaaga tctgtgctgc aagaaattgg tgatcgtatc ttgagtagca catcagtttc
    18301 atctagggca caaacgattc aacctgtgaa taactacgaa ttcaatttca caattgatgg
    18361 taacgcagat gaggttacta tgaagcaaac aactcaacaa atcattgata gcattacaaa
    18421 agttcaaaat gataatgctt cggcatggcg ttaaacagga gagtatttct cctgtttttt
    18481 tagtattaaa aaggatgtga aaaaatgact gattgtatac attctataat cgatggattt
    18541 cctgattatt tgcataaatt ggctttagct gaaagaccaa ccataccttc tccaaaaaga
    18601 cagagagttg aaacttctgt tttaggaagg ttaggtggct tagtacaaga ttactcgttt
    18661 gaagacatgt cgtttacatt gcactataac tatttagagg atgtggaaga ccatcaagcg
    18721 ttcaagcaat cgttttatat catgcgtcat tggttaaatt atgcaaagaa attagaattc
    18781 tctgatgatc ccaacgtcta ttatgttatc cagactatcg atattgggga tgcagaaaac
    18841 gatattgttg aatggggaga gttcgatgta aatattactg cgaaaccatt cgcaagagtt
    18901 caagaagatg tacctataac cgtagataaa ccacagtcat ttaacttgct gaataatagt
    18961 ttagaagaaa gttttccaaa gattatcatc actccttcag ctacttcatg ccagttcatc
    19021 ttaaatgatt atgtgtttag ttttgaaggc ttagtagtag gaactgacgt agtcattgat
    19081 agtgatttga tgctttgcta cgaagagcaa tcggacggag atattttaga tcggtccaac
    19141 aaaatgaaga ccatgcaata tccgacattg caagtggata ttaattattt taattgtact
    19201 ggtttgagca aaatacaaat ttatcgtaat gggttaaggt aggtgaaata gatgatcgat
    19261 aatttaataa ctatttacga taaaaacgac gcgaataatt tagctgaaca tttatatgat
    19321 acgcaaggtt taggcgcttt gtcagactgg ttaacagcta ctgttagcaa taaattaaac
    19381 ggagccgaga tatttcaggg tacttatcca ataagcggaa ctaatgcaga tttgattgta
    19441 gaaggacgta ttattcagtg ttatgtagat gaaaatcgag caaaacagcg tctacggatt
    19501 tattatgcaa agacttctgt aataggcaat acgatagaag taaaagctga acctattttc
    19561 aatgatataa gaaaatcggt gttgaataaa tatgacagtg gaacagaaaa gatcactgct
    19621 agtcaggcat ggcaaaacgc aaaaacttta gcgaaaccag ttattccttc acagttttct
    19681 ttctcgtcat tagtagatac gcttgctaat gtgaagatag aaaaggcgaa ttttttagaa
    19741 ttctttggtg gaaaagaggg atctattcta gatcgatttc atggggaatt tctaaaagat
    19801 aataacacat tacgtcatga aaaaaggcta ggcacggatc ataaaatcaa agcgatttat
    19861 actaaaaact taactggtct tgacttagag attgatgctc aaagtgtttt agttggagtt
    19921 tatccattca ttagcagctc ttcagaagga gaagacgaga tcactctacc agaagaagtt
    19981 attttcacgg attacgtgga tgattatcct gctggatatg tttcttttgt tgattttaaa
    20041 gacaaagcga ctgatgtagc cacattaagg gaagctgcta aagactggtt gaaaacaaac
    20101 atagataaac aaaaaccaca agtgagtggt tcgattgaat tagtaccatt gaggcatcaa
    20161 agaggctatg aaaaatttgt tgatctagaa aaagtttcga tgggtgacgg agtagatgtg
    20221 tatcatccac agttaaaagt gaatatgtca gcgagaattg tggaatatac gtttaatgtt
    20281 ctaaccaatt catacgataa attagttgta ggaaacgtca aaacaaactt cttagaaaac
    20341 acagagaata atgtcagcaa tttgattaat gatgccattg atcaattgaa aaacggtggc
    20401 gaaatcagtg atttactcaa tgatattgta gatcatcaaa ctgatatgat tactggtcaa
    20461 aatggtggtt atgttttatt agatcctaaa gaagcgccta gtcgtatttt gattatggac
    20521 acaccagata agaataccgc aaggaatgtt ttacaaatca acaatgctgg tattggtttt
    20581 tctaaaactg gcattaatgg aacatatgaa acggcatgga cgttagatgg cggattcaat
    20641 gcctcgttta ttacggctgg tgaaatagta ggaattacta ttagaggtac tacattaatt
    20701 agtgatggtg ctgattatag aacaagtatt gctaatggca aaatgacttg gtactcaaaa
    20761 aaagttaaca aagatattat ggagctagaa gcacgtgatt atgtaagtgc tgatgccggt
    20821 attgtatcat acaccatgaa aactggtggt ggtttcatga ttagaaatcc acagggtaac
    20881 ttggttttta gtacgtggga taatggtaat aacagaccgt ttctatcttt tggtgcgccc
    20941 aatttcaggt atagcaatgc tagttatgta acttctggcg acggtagttc tttaagcatt
    21001 aatggtagtg cgggtaactc atgggaattt aaggtagctg gtaggactat gaaatttact
    21061 agtgatggta tgctaacgtt accaggttgt ttttttggtt catgggaaga tgggaaactt
    21121 gctaggtttg aacaatcaac ggtacaagta tataaagatt ttactgttag aggtactaaa
    21181 aactcaactg taccaacaga acattatgga caacgactat tgaacgctta tgaaactcca
    21241 gaatattatt tcgctgatta tggggaagcc gttacaggtg acgatggtaa agttcgtgtt
    21301 gatattgacc ccatgtttgc tgaaacagta aatctaagtc ggtatatgac acatgtgaca
    21361 cctacagaac tagttttgtg tgctgttact catgaagata ttgaccattt catcattgaa
    21421 actagtaagc caaacgtatt agttagatgg aatttagtgg cacaccgtct agggtatgaa
    21481 gatattagat taaaagagga tacagcatat gatagcacag tgcttgacca aaaacgtttt
    21541 taaaacgaag acaaggaggt atataaatgg ctagcagttt atataatttg gctttagatt
    21601 tcagcaaaga attaaactac accaaagcta ttatggctcg tcaaggtgat aaagggatta
    21661 cggtgactgt taaaccattt ttaaatggct tgcagatgga tacgagtggc ggaacattta
    21721 ctttaaaagg aacaacacca tctaaccgtt atgtagatag tgttgcaact agcgtaacta
    21781 gtgaagaagt catattttct cttgatggca catttatgag tgaagcagga tattataaac
    21841 actgctacgt agaatataga aaaggtaatc aaattttaac aacgcaagac atcatttttt
    21901 tctcactagg agtgtctgac atttcgcaag gccaagccga tgaatacgtt tcgcaattgg
    21961 aagagttgat tcgaaagtat aacgaaactt ttgatgcttt tatggctgaa attaaaggta
    22021 gagtggatag cttaaatcaa cagattactg atttaactgg tcaagctaaa acgctacaag
    22081 acaagttaga tgctctgaaa gaagaaattt ctaaattagg taacttgcaa gtcatgtaca
    22141 gtaacagcat cgacttcggg ggctatgatt attc
//
LOCUS       DACJGB010000052        21203 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.052, whole genome shotgun sequence.
ACCESSION   DACJGB010000052 DACJGB010000000
VERSION     DACJGB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.052"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..210)
                     /locus_tag="IYI34_001983"
     CDS             complement(<1..210)
                     /locus_tag="IYI34_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR0559846.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="IYI34_001984"
     CDS             complement(309..623)
                     /locus_tag="IYI34_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559847.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="IYI34_001985"
     CDS             complement(691..1185)
                     /locus_tag="IYI34_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559848.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1178..1492)
                     /locus_tag="IYI34_001986"
     CDS             complement(1178..1492)
                     /locus_tag="IYI34_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559849.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            complement(1708..3099)
                     /locus_tag="IYI34_001987"
     CDS             complement(1708..3099)
                     /locus_tag="IYI34_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAR0559850.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="IYI34_001988"
     CDS             complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="IYI34_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAR0559851.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3699..3896)
                     /locus_tag="IYI34_001989"
     CDS             complement(3699..3896)
                     /locus_tag="IYI34_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559852.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3887..6565)
                     /locus_tag="IYI34_001990"
     CDS             complement(3887..6565)
                     /locus_tag="IYI34_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559853.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6552..6770)
                     /locus_tag="IYI34_001991"
     CDS             complement(6552..6770)
                     /locus_tag="IYI34_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559854.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6846..7898)
                     /locus_tag="IYI34_001992"
     CDS             complement(6846..7898)
                     /locus_tag="IYI34_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAR0559855.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDSSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8051..8377)
                     /locus_tag="IYI34_001993"
     CDS             complement(8051..8377)
                     /locus_tag="IYI34_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559856.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8389..8589)
                     /locus_tag="IYI34_001994"
     CDS             complement(8389..8589)
                     /locus_tag="IYI34_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0559857.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8664..9314
                     /locus_tag="IYI34_001995"
     CDS             8664..9314
                     /locus_tag="IYI34_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0559858.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9369..10535
                     /locus_tag="IYI34_001996"
     CDS             9369..10535
                     /locus_tag="IYI34_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0559859.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            10952..14179
                     /gene="fss3"
                     /locus_tag="IYI34_001997"
     CDS             10952..14179
                     /gene="fss3"
                     /locus_tag="IYI34_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAR0559860.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            14291..14605
                     /locus_tag="IYI34_001998"
     CDS             14291..14605
                     /locus_tag="IYI34_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR0559861.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14618..14992
                     /locus_tag="IYI34_001999"
     CDS             14618..14992
                     /locus_tag="IYI34_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR0559862.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            15002..15346
                     /locus_tag="IYI34_002000"
     CDS             15002..15346
                     /locus_tag="IYI34_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAR0559863.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            15415..16431
                     /locus_tag="IYI34_002001"
     CDS             15415..16431
                     /locus_tag="IYI34_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAR0559864.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            17025..18899
                     /gene="ltrA"
                     /locus_tag="IYI34_002002"
     CDS             17025..18899
                     /gene="ltrA"
                     /locus_tag="IYI34_002002"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR0559865.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            19025..19381
                     /locus_tag="IYI34_002003"
     CDS             19025..19381
                     /locus_tag="IYI34_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAR0559866.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            19426..20568
                     /gene="dcm"
                     /locus_tag="IYI34_002004"
     CDS             19426..20568
                     /gene="dcm"
                     /locus_tag="IYI34_002004"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAR0559867.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVKDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            20593..20847
                     /locus_tag="IYI34_002005"
     CDS             20593..20847
                     /locus_tag="IYI34_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559868.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
BASE COUNT         6810 a         3773 c         3774 g         6846 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttgtattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattt gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ttcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 gccatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttctag attgtttatc
     2341 tcttcctaaa gtaattaact cacctctacg ctcatagaaa tttacacctt gtttgtaaag
     2401 aacatagcca agtttgttat ttgtttttga aaattgataa acttcaatgt tatattcttt
     2461 tgccaaatga tccgaaaaat cttcaaaaga attcacttcg catttttcca acgttgcttc
     2521 gatcgcattt tgcacaaact cttgataatt aatttttcca tatttcatct tagaaggatc
     2581 gtttgaataa agattggttc tcttttctaa gttttcatag tactcattat tcactgattc
     2641 aagaccatgt tcattgagga cttgatcgtt aatttttcgc attagttcta aatctttagg
     2701 gtcaatacgc aaccttttac atgtttccat gttaatatta ttaatgacaa gatgactatg
     2761 aaaatgatct ttatcattgt gacttgcaac aatgatttgc gaatttggaa aagctttctc
     2821 tgcaaattcg acagctattt cgtgcatttt atcaatactt aactctttag aagtactctt
     2881 atcaaacgaa tgaataacat gaaacgcttg tgctatatct ttagatttat tcatcttggt
     2941 acgtactaaa ttcatttctt gcagtgcttc ctcactatca tcactacaat ttattcctga
     3001 gactgcaaca catttttctt tttttgaagg attagctgca tattgtaata gcgaggataa
     3061 atttttcttg cgtcctttcg atgttatagt agctcccaaa gttgtttcac ctcatctttt
     3121 aaacttgcaa taatctgaat tgagtgaatt aaattccgat caactttttg ggaatactct
     3181 tctttcgtac ccggtctagt ttgcttttct atccaatatt cgaaatattc tttttctact
     3241 tctgataatt taaaaaaaga gggggcactc cgattggact ccaaaaaaat gtcatctgga
     3301 taactaatca tcaacttttt tgctatgcta ttcgcaagtt catctttaaa aatgttgtaa
     3361 cggctagcat caaaactaaa atgtaggctc ttttcttttt tatcttcaga ttcttctcta
     3421 aaatatttag caatgatatt taatgccttt accgcttgat tcaaattgtt accaatttga
     3481 tttaactgtt tggttaaaac taccctatca tcttcagcta acaacggcgg ttcatatttg
     3541 agtcccttta accgctcacg gcaaacgtct gctctagaaa ttcccctttg ttttgcaatc
     3601 aatgtcagtt caagataagt ctttttatcg acacgaaaat ttaaggcctc actatgtttg
     3661 cgattttttt cgtacatttt ttgtttatca tccattgatt atcccctcac atttttttct
     3721 agagagcaac atttgaaatt gctctctatt tcatttttca tctttaattc ttcttgaatc
     3781 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgcgttctt tactgattca
     3841 cataattcgt caagttctaa gaaatcatgc gtactaatct ttagaattaa ttgcatgaca
     3901 atccccccct tttatttctt aataaattat cgaaaagtgg attaggatca ttaagtgtat
     3961 ctctgaataa ctcattgtct tcataagtga atcttcgtct tgattttgat cccttatcat
     4021 tttcacggat ttcactatca ataatcactg tttcatgatt tgaatcaaat tttttcgctg
     4081 actttctgtc ttcaacgata ttgatttttt gtgttttttc tttcatttct ccactatctt
     4141 tttgaatgtt ctcccgaaca tctactgttt tgaatccttg actacttgta gaactttgta
     4201 cattttctcg gacatctact gtttttggtt gacttgcact gctaccttgc acattctctc
     4261 ggacatccac tgatttaaac ccttgactac ttgtagaatt ttgtacattt tctcgaacat
     4321 ctactgtttt tggttgactt gtactgctac tttgcagatt ttctcgaaca tcaattgtct
     4381 tagtaccttg agcatttgaa gaactttgta aattctctct tacatcaaca ttccgtccaa
     4441 catgaccatt cagtgaacct tgaatatttt ctcggacatc tactgatcgg ccaccatgac
     4501 tacttgtaga attttgtgga ttttctttga ctaatttttc taatcctgta tttgaatttt
     4561 tagcttcttg aattctttct ttggtcaacg cttcttttcc gtcaatatta gcgcctgtcc
     4621 gcccttctac acgtttcctt aacattattt cctcgcgatt cattgcttca ggcgtgtatt
     4681 tcgcttcatg acgcttctga ttaattaatt cttcagcaga cttagcttga ccgtgcatag
     4741 catttttacg ttcttcaata tcttgttgga ctttttgctg aattgcgctt gcaggattat
     4801 tattcgattc agcattattt ttcattgctt cacgcatacg ttcagaacta gacataattc
     4861 tatcggcatt ttcaacttta gcaccatctt ttctatctga aagacgtgtt ggcacggaat
     4921 cagcatattt tccagtttca tcttctttat ttttagctaa cataggtttg ctatttttat
     4981 taatagcgga cactgtacca tcatctaaac cttctttaaa cttattcttg gtatcattag
     5041 ctatatcacg catactctta aatggttttg ttgctctttc actagccatt gtagctccat
     5101 ctgaagctaa accggataat ccacgttcat gggcatatcc caaagctctt ccagttttgc
     5161 cagctttttt tgatagagtt tcgccgaaat tcttttctgg ttttcgatct tctccttctc
     5221 cgcttccttt agcacgatta aatccatttg atccttttct aaacagcatt gcgcccattc
     5281 caaaagcaga agcaagttgc ccatagcctt ctttgagtcc aatatcaaca ccaaagtatc
     5341 tcatgactgt tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt
     5401 ttataaaaat atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa
     5461 acattgataa cccaaatcct tgaaacccaa cagttaagta acattgtaga atatctgata
     5521 gaacaacttt tgaacgctgt cctgtctcaa tatctgttgc aaaaacaaga acacctaaca
     5581 cacgtttaaa tgcaagttct agtattgctg taatgatcac aaacgcgctg aacacatagg
     5641 cgattgctaa cgctgtcaac ccaattgaaa tcccccatag atttgcttgg tatcggtaat
     5701 agcctgattt caagctatca gaaaatgttg ataagaagtt gtcagaaaac tttgtagcaa
     5761 caaattcatt attagaattt tcaactaatt cataacgaag attgtccgca attggatttt
     5821 ctttttccaa tttatcaatc actttatccg ttaacacttg tgtgagatcc gtctgttgaa
     5881 aattttttcg tgttaaatta tttcgatcct ccgttttatc aatgcttgag tactgatctg
     5941 ttttattaat atatgctaaa tctgttactc cttgttttat caatgaccaa gcgactccgt
     6001 catctgaatt tgtaatcgta gtagagtcct tataaaatgt tttagcaaat tgaataccac
     6061 tagaaatcat ggttggcatt aaaagtatta atgctataga catcactacg ttcattccaa
     6121 ccgattttaa atccactgtt ccttttccag taatcatttt gtaaccaaca aacatcaatg
     6181 aaaggatcat taatccaatg acaattgtat ttacggctga ttgataaaca ttatttagcc
     6241 ctgtactttc aatgaaattg aacaaatcca gcacatctgg aatcatattt tgaatcgaat
     6301 ctgtcacagt aaacatgcct tttaccacag cccatccgag ccaaatcccg aactgactaa
     6361 ggaaatctcc attgtacttt tttaggtaag gtctataaag agaattattc aaaaactctg
     6421 tttgactttt tgtgtcatca aaccaattga aaatactcgc atgagcaata ttacttgtaa
     6481 aaataatcat agctatcaga acaagaaaat taacaaccaa ccaatttatt tttttattgg
     6541 caatcttttt ttcaattatt ttcatcgtta cctcccttag cccattttct gaggctctca
     6601 gagagcttct gagccatttt ttgattaatt atgacatgag ttacacaatt atcattttca
     6661 gctcttaaat cgagaactat agtgttgaag caaatagtct cgattaattc gagactatct
     6721 gtattttgat ctagacacca aacttgatct attaaacgat ttttaatcaa aataacccct
     6781 cctatattca aagttctcaa atgaagtgga gatacccctc catttcattt gactcaattc
     6841 tattcttatt gcttatctat tccttcgctg atttgcaatt caattagttc taacgcttta
     6901 ttttttagag aagcaaagat ataatcgtct ttttgtttga tacgatgatc tgtttttgct
     6961 cttcgtatca agtcacgtaa agtcattgat agctgttctc taacatgatc tacaaaatac
     7021 ttgttattac aaatttcata gataattttc agttgaattt tttgttcttt ttcagcagat
     7081 ctaatcgcat atttgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta
     7141 gccagtttta agcaattcac aatactttct ccccatattt gtttaagaag attttcttca
     7201 ataaatgttt cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaga
     7261 agatcatcat tatgtgattc tttaatatct ttactatctt tataagactt atataattct
     7321 agttcatttt ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg
     7381 catagttcaa attttgaact atcaacagaa tattgatttg ttccatagtt caaattttga
     7441 actatcaagt ttttttgtct attttcttga tgggttatct tattaatttt ataaacatca
     7501 tctttgcaca gaacaggctt taacaaataa taaaaatttg ggaacatttg cattttactt
     7561 ttattgaagc cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct
     7621 gataagattg tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc
     7681 aaaaaaattt tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct
     7741 tttagtaaca tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg
     7801 tttaaaaaaa taggatattg atagtataat tctgcatata tatcagaatt agaatagcct
     7861 tgcttttcaa ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt
     7921 atttgctata atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg
     7981 atctgcaaac tgacgggaac actcttttta attttttcta cttcatcaaa tttaaaaaac
     8041 gaggtgtttt ttaaatttga tgactcaata tccatttttt gcattcttct tttgaaaaca
     8101 actccatttt ttctaataaa ataactggca atcccattct tttaaatttc aaaaaagttg
     8161 tatatgatac attcaaatat ttagccatct cttttccttt tagaaatggt ctttctaatc
     8221 cggtatcaat tcttgcttga tcaatagcct cttttgtcat ttcaaaaaca taatttttca
     8281 attgattcgt ttgttgttta tctaacatta ctgtaattcc tgcatccatt atttttctcc
     8341 tctcattttc tatttcttca atcagccaac tcgccaagtt gtatttattt agattttcaa
     8401 atcattaagt aaccaattta ccaacttttg ataagtagat gctctaatac tttctttgct
     8461 cccattttcg attttgacat atgttttccg atttatacct atcaattttg ctgcatcgtt
     8521 tttagtaagt ataagatcgg ctctatgtct tcttaagctt ctaatgaatt gatcattaag
     8581 agttatcatt cagcgcctcc tttcaaatta ttcactttgt ggtttttatc ttaataagca
     8641 cattttaaac cacaaaacga gtaatatcaa ttaaaatatt actcaatttg tggttttcat
     8701 gctataatag tttggaattt actatagggg gcgttacttt gaatagaata aaattttata
     8761 ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt aactatacaa
     8821 ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca tggaatatcc
     8881 ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa acaaattcca
     8941 tagaacaacc taatcttgat agaataaaaa ctgctcgttt agaaaaaaag gacaaaatag
     9001 tcaagaatat tgccagtctt gactcagatt attttactga attatctaat ctatactttt
     9061 ccatgacttc tttacttgaa gtcaaacttc aagaagatga ctccgacttt ctttttttaa
     9121 aaagtattct agaggactcc tttgaataca cgattctatt tgccaaacaa aagaagttta
     9181 ttaataaagc tttaaactta aatatcgatg aaagaaaaga gttagaatct gagttatttg
     9241 gcaacttaac ccaagtacag gttaacaatc taaaaagaga gataacagct cgattaatat
     9301 caaaacttca ataaaactaa ttattcaatc agccaactcg ccaagttatc agattggaga
     9361 gattagttat gtcaatacaa aagtacacaa caaaaagcgg tgcacgatat agtgtccgct
     9421 tgtatgttcg taaagatgaa aatggaaagc aagaatacta tgtaaaaaaa gggtttaaaa
     9481 ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa gataccggct
     9541 atcataagcc ttctaatgac ttgtttataa cagtgtatga agaatggttg ccttcttata
     9601 ggaatcgtgt acaagaaacg acatatcaac gtacaaaaga ccttttccgc ttgcatatac
     9661 taccaaaatt cgggaataag tccatttcta agattactcc tgttcactgt cagaatgcag
     9721 tcaatgaatg ggctttaaaa ttcaagaata tcaaacagct taagtcttac agctctcaaa
     9781 tatttgagta tgccattttt gcagagctat tacaccgaaa tcctatggca aatacaaatc
     9841 taccaaagcg ggaaaagaaa gaaaatgaaa attacttttc attagaagaa ctaaaagctt
     9901 tttgggatat tctcaataaa gaagagcctt taaaacatat tttaatcttc cagcttttga
     9961 ttactactgg tatacgtaaa ggagagctag ctgctcttca ttggtctgac atttattttg
    10021 aagaacagct aatgtatgtt agaaagagct atgcaactat tcgaaatgaa gagaagaatg
    10081 caagaagaaa aactgtcagg attcaaaaaa atacaaaaaa tatatcttcc gaacgaatat
    10141 taccaattga tgcacaaact attgaaatgt taaaaatctg gaaaaaagaa caagctcgag
    10201 agcttttgca gtttggcata aatacaaata caaaaaatca acttatcttt acctatgtaa
    10261 atgcagacgg tgacattaat caacctttgc atgcggacta ttcaaataat atattgaaac
    10321 gtttagagaa gaaatataaa tttaagcacg ttactattca tggattccgg cacactcacg
    10381 ctactcttct actagaaggt ggagcaagta tcaaagaaac acaagatcga ttgggtcata
    10441 aaaatgctga aactaccctt aacacttatt ctcatgttac tgaaaaagct caacgaaatg
    10501 cgttagaaaa attcattgct tacactggtt tataatattg tcattacctt tctcattacc
    10561 agaacgaaaa aaagacacct tctcgcaatg agaaagtgtc tgaaatcgtt ggtataacaa
    10621 cgattaacgt tttgaaaact gtgaagcttt acgtgctttc ttaagacctg gttttttctt
    10681 cgtatcattg aataagtttg aaaagccttg atttataaag gtttttccgt aaatatattt
    10741 tgttatattt gattaaattt gaaatgtttg tgttctaaaa tgtgttctaa tttaaaggca
    10801 gtatatttcg tattttatat taaattttta ggctcattca ttctcaaatc cattctattt
    10861 agtcaataaa tattttaaaa agactttata aacaaagatg ttcaagcgag catcttattt
    10921 ttttgtgcct aatttgaaag gagaacattt catgtataaa aaacaaaaaa agaaattatg
    10981 tgtattaact attttgagtt tggctgttca atcaattgta ggagcaattg ctcctacgat
    11041 tgcttttgct gatgaaatta cccatccaca aacggtaacc gtagaactag atttagcaca
    11101 tcaatatgct gttgaaggaa cttttagtga tggtcgtcca atgtcagaag tcactgttcc
    11161 gcattatgca gtgtacaacg gcgttaaaca agatattttc tgtgttgaac caggagtccc
    11221 gatttacaat gagttcactc ctggatatga gaagaacccg ttaccagata tgtccgataa
    11281 agccaaatta gtttccgttt tatggaaaaa agtaggtaca gatgcagata ctcatattgt
    11341 ggcacagaag atgatctggc aagaagttaa tgggtatacc cttcactcaa taaaacgatc
    11401 agatggtagt gccgtaaata tcgcagccat tgaggcaaaa atcaatcaag ccattgccga
    11461 ttatcagaag aaaccaagct tccataatag tacggctaaa acagtattag ggcaatcgac
    11521 tactgtgacg gatacgaata atctgaattt gtcagagttc gatgaggttg tggaaaatac
    11581 cgcaaacatt gattatcgtg tgaatggcaa tcagttgata atcagcccaa atgccaattc
    11641 aaaagaaagt ggtgtgttga cgcttaagaa atctgctggt accggaacac ctgtcgccta
    11701 taaaaaggct ggccaacaga ctttaatggc cggggcgatc gataagccga acacttatac
    11761 ggtcaaaatt gatgtggaaa tggagggttc tttgaagatt aaaaaagttg ataaagaatc
    11821 gggtgatatt ataccaggaa cggttttcca tttagacttt ggaaaaactt tacccgcaaa
    11881 agacgtgacc accgataaag aaggcattgc gaccttagat gagattcctc atggtacaaa
    11941 ggtaaccatt actgaaaaat cggtgccggc gccttatacg attgatacca cacccatgac
    12001 tgccacaatt aaagcaggtg aaaccatttc agtgacttca aaaaatacgc gagaaaaagg
    12061 tcaaattatt ctggataaaa ccggaattga aacagggagt gatctttgga acgataatta
    12121 ctctttagcg ggcaatacgt ttgccattcg caaggatagc ccaactggtg aaatcgtcca
    12181 agaaatgacc acggatgaaa aaggtcatgc agaaacacca aaagagattg ccaatgcgtt
    12241 ggaattggga acctactatg tgactgaaac caaggctagt catggctttg tgaatacttt
    12301 caaaccagta aaagtcgaat tgaattatgt caatcaaaca gtggctcttg ttaccagtaa
    12361 cgtaaaaggg caaaaccaag aaattactgg tgaaaccact ttgacaaaag aagacaaaga
    12421 taccggtcat gaaactcaag ggaaagctga gtttaaaaga gctgaataca ccttgtttac
    12481 tgttaaggat ggaaaagcgg tcaaatggac cgagaacttt aaaccagaat tgttgaaggg
    12541 aacgaaagct tctgatgaaa ccgtaacttt gactttagat gaaaagaatc aagttgccgt
    12601 taaacactta gcgattaatg agtattactg gcaagaaacc aaagcgcctg aaggatatac
    12661 cttggatgaa acaaagtatc ctgtatccat caaaaaggtg gatgatagcg aaaaaaatgc
    12721 tgtgattact cgagatgttg cggcaaaaga acaagtgatt cgctttggct ttgatttctt
    12781 taaatttgct ggatcaacta ctggcactgc cgaaactggc ttcaatgatc taacctttaa
    12841 agtgtcgcca ttggaaggaa ccaatgaaat caccggtgcc gaagatgaag cgaccacagc
    12901 ttataacgag caattagggt ttgatggtta tggcaagttt gaaaatcttc cttatgggga
    12961 ttatttactt gaagaagtag aagccccaga aggttttcaa aagattacgc cattagaaat
    13021 ccgttctact tttaaagaaa ataaagagga ttataccaag agtgattata tctttactat
    13081 taccgaaaaa gggcaaaagc aaccgattaa aacggtaacg gtcccttatg ggaatctaac
    13141 caatgagaat ttctcagtca gtttgaaccg tttgatgctt tatgatttgc ctgaggaaga
    13201 agatagctta acttctcttg cgacttggaa agaaggagat aaagaattga ctactcttga
    13261 ttctaccgag ctagtcgata aattgagcta taatctacat gaaatcaaag aagactggta
    13321 tatcgtagct caagccatcg atgtagatgc tacaaaagct gcccaagaaa aagataaaaa
    13381 agccaaaccg gtggcgattg cggaaacaac cgcaacttta gcgaataaag agaaaacagg
    13441 aacttgggag attctgcata aattaactgt cgaacaagtt ttagacaaga ccattgtctt
    13501 attcaactat gtgtatgaaa gcaaagaagc ctatgaagct ggtgatgagc cggtagcgaa
    13561 ggatgctagc ttgaacaatc aagcgcaaac tgttaattgt atggtggaac gtcatgtttc
    13621 catccaaacc aaagcgcact tagaagaagg ttctcagacc tttactcatg gtgacgtggt
    13681 agatatgttt gatgatgtat ccattaccca tgatgtacta gatggctcaa aagaagcttt
    13741 tgaaacaatt ctgtacgctt tatttccaga tggtacaagt aaagaaattt ggaaatctgg
    13801 caagattgat tacgaagtga atgacaaaga atttaccaaa accgtacttg cggaaaaagt
    13861 ggatacaagt aaatatccag aaggtacaag ttttaccttc aaagaaatta actatgacaa
    13921 agatgggaat atcaatggaa aacacaatga agatttgaaa gaaaaatctc aaactttaac
    13981 gcccaaaaaa gttccgacaa caccaagtac accagaacaa tctgaaatgc caactgttcc
    14041 aagtggctct caagaatcta gtcctacagt gaaggcattc cctcaaaccg gggagaaaac
    14101 ttccaatgtg ctactgttca ttggctttag cttgatcttt gcgacggctg gctattattt
    14161 ttggaatcgt cggaactaag gtgataagat gaaacatgta aatagtagaa aacaatacgc
    14221 gcgaccgcct cctaaaaaag tggtcgcgct atttttgtat tcaaaactaa caatgaaaag
    14281 aggaaaaaat atggaattga aatttgttgt accagatatg gtcgagacgt tcggaaaact
    14341 aacatacggt ggtgaaggtg aagttctatc ggaagggtat ggtcgaaata ctacaattgt
    14401 tggtcgaagt taccatctgt tttcaaataa acaacgggcc gatgatattg aagtggtggt
    14461 agctgcggaa gccggcgaaa aggattttga ccaagatcaa ccactaaaag ctgtaaatcc
    14521 ccacttaatt gccaaaggct atgaaattga gaatcgtggg tttaccgatt acgtgttgta
    14581 tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag aaggtattgt
    14641 cgtagattca ggattaacct ttggcaagtt gcgtttttcc gcattacgtc gagaagtacg
    14701 caagcaaaat gaggacggaa cggttagtaa cgaggtaaaa gaacggacct ataatttgaa
    14761 atcctccgcg caaggtcgaa tgatccaagt cagtattcca gcaaatgtac ccttacgcga
    14821 gtttgcttat gatgcagagg tagagctggt caatccaatc gtggatacgg tcgccaatta
    14881 tgtttttcgg gaaggaacca ccgttaattg gtttatcaag gcagatgatt tagtcttgaa
    14941 acgacaactg aatcaaggaa aacctacgaa ccaaaatgaa ggaaagaaat aggtgaagaa
    15001 tatggaaatt aatgttgaac atattttaga ttgtctcgat caatttggca aaggtgagct
    15061 aacggaggaa caactcacta acgcattaac ctatgatgaa aaaatgtttc tgattatgca
    15121 tcaagggtta ctggaagtag gtaacgatgc caaagaagac tttgatgtac tggattggtt
    15181 aggggaagaa gagtctttct ttatggttgt cgaggtaaat gaaagtttat gtcggcaggc
    15241 agaatctgtt ttggaagaaa tcgatgtgga gatgccggat gccatcgaaa gatttttaaa
    15301 acaattagtg gaaacgaaac aacttcccgt agtagtaaat gactagcata actttttgaa
    15361 attgcccaat gaaatcctat tgggcttttt cgactggagg tggaaaccat caaaatgtac
    15421 aaaggacatc gcatacgcgc aggagatcaa cacttgattt atcactttgt tcttggctgg
    15481 ctaatctttt tgttcattgg ttggatgagc gtcttttatt tacaagaact tagacaattt
    15541 gatatttcta aggtatcatt ttccactagt gaaattggct ggtctatgaa ggatgtaatc
    15601 tgcttgctag gaagtctcgc tctttcagga agcatgatgt tactttatat acactttttt
    15661 caggatcatt ggcgaagtct ttggcatcga caaaagttag ctcggatgat tctagaaaac
    15721 cattggtatg aagtaaagca gactcaaggt gaaggttttt tcaaagattt gaatagtagt
    15781 tggaccaaag agagtatcag ttacttcccc aaaatttatt atcgaatgaa agatggctta
    15841 ctttctattc gcgttcaaat ttcactggga aaatatcagg atcagctatt gaagttggaa
    15901 aataagttag aaagcggact gtattgtgag ttagtagaaa aagaactcaa agactcctat
    15961 gtggaataca ccttgttata cgatatgatt gccaatcgaa ttgggataga agaaatagtg
    16021 gcagaaaatg gggcgttgcg attgatgaaa aatcaagtat gggcgtatga ttccttgccg
    16081 catatgttaa ttgctggcgg cacaggtggt gggaagacct atttcttact caccattatt
    16141 caagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa tgcggacctc
    16201 gccgatttag gtacgataat gcctcacgta tactcccaaa aagaagaaat ttctgaatgt
    16261 gtggaagatt tttacgaacg catgatagct cgtagtaggt caatgaaaga aatgcccaac
    16321 tacaaaacag gagagaatta tgcgtatctc ggacttccgc ccaacttttt aatctttgat
    16381 gaatacgtgg cttatatggg tgcgaatcgt tttcctacaa gcatcgaata gatgtgaaat
    16441 ggaagcctat tgagattatc aatagtgaca actaatgttt tgagagatgg aatgaaagcc
    16501 ttcaagttga gctgaaacat ccccgctaat actcctacat gctctaatca atgaagtaga
    16561 ttggttttgg tatgaagcta ggtgaagtcg tctgaaatct gcctaagtgc ttgagcataa
    16621 ggctgactat agggcgggcg gctgaaaaag ataactatgg taagaatgtc tgaaaatgac
    16681 tgacgaatct gcgaatgtac gggtctaaaa ggctgatctt attgaaagat aggaacatac
    16741 ggacgtatgg tgagataccg acaagtaagg ttccatgata tgaaaatcgg gatagctaac
    16801 gaagaagcta taatttcaca ggctcatagt agatacctaa ggttatacat gatagctaaa
    16861 aacattggaa cgatgtaagc agaaaactat caattaaatg actacttcta gataggtgca
    16921 tataagtaat aacgaaattg ctttgtttct gtgaaagtaa gggcacagta ccgatgaagc
    16981 gtctaacaaa tgtggaggga tagccctaag tctatttaat aacaatgaat gttgaaacag
    17041 atgaagttca aggatctggt aagaacgtgg gaaaatccgt tatggaggtg ccacgcttga
    17101 acacaaaaat acgttactgg gaatattatg atctacaaga aacattcgat cagctattta
    17161 ctcaaagtag aaacgggaaa aagttttatc aattatatga gttaatcatt tctgaaaata
    17221 atattctttt agcataccgt actatcaagg caaacaaggg gtcttcgaca ccaggaacgg
    17281 attccttcac aattgataac tataaggaaa tgaaccaagc agagtttatc catcttattc
    17341 tcagtcattt agaaaattac aaaccaaaat ccataaagcg agtaatgata cctaaaccca
    17401 atggtgagaa acgcccatta ggaattccat gcatgattga tcgaatgatc caacagatgt
    17461 tcaagcagat actggagcca atatgtgaag ctaagtttta tgaacatagc tacggtttca
    17521 gaccattgag aagtgcaaag catgctctag gtcgcattat gtatctcata aacatcagta
    17581 aaatgcacta tgcagtagac attgatatca aaggtttctt tgataatgta aaccaccgtt
    17641 tactgataaa acaattatgg aatataggaa tttgtgacaa acaagtctta gctattcttt
    17701 caaaatctct gaaatcaccc attcaaggag aaggtatccc gagcaaagga acgatccaag
    17761 gaggtatcat ttctccacta ttatctaatg ttgttctaaa cgacttagac cattgggtct
    17821 caaagcagtg gcataccttt gaaacaaagt atccctatac taaaggttac aataaatttc
    17881 gtgctctaag agatacaaat ctaaagcaag gctatattgt caggtacgca gatgacttta
    17941 aaatcatgac taatgattat ccgacagcat taaaatggtt ccacgctgtc aagctttacc
    18001 taaaagacag acttaaatta gatatttcca atgagaagtc taaaatcgtc aatttgcgta
    18061 aatgtaagtc ggaattttta ggattcgcta tttgcgtgaa acaaaaaggg aaaaaatggg
    18121 tttgtaattc acgtatttct aacaaaaaaa aggatcagat taaagaagag attaaacaaa
    18181 gaatcaaaga tattcaaaag agtcctacag cacaaaatgc tctcctgttt aatgctctcg
    18241 tgctcggtgt tcataattat ttcaggtatg ccactcatat taatttagat ttgaatagga
    18301 tgaactacgt tctttcattc actacttaca accgattatc aaattgtagt acaagatgtt
    18361 accccaacaa agcttcccca acttacaaaa agttttactc aacaacgatg aaaacgtata
    18421 aagtagctgg agtctatttg taccctcttt gcgacgtatc tacaaaaaca atttacggat
    18481 ttaatacgga ggatacaccg tttactccgt ttggtcgtca aagattggag cacaaaagcc
    18541 tacagtctct tgtgttccaa gaattaagaa aattgatgga atcgaaaata ccgaatagaa
    18601 cggttgagta tttagatagt agaatatctc gttactcaat gaaatcaggc aagtgtgaga
    18661 ttacaaaaca atttttgcca gcaaaagccg ttcactgtca tcactatttg ccaaaaagtc
    18721 ttggtggaga cgacaaattc gataacttga gaattattca taaggatatc catcttctga
    18781 tccatacaac aaataaaatg ataatcgatc actacgtgca tgaactgaaa ctggtgccag
    18841 agcaaatagc aaaaatcaac ctttatcgaa agatgtgtaa tctgcaaaac attcaataga
    18901 aaataaatag atggaacgcc gtatgaggtg aaagtctcac gtacggtgtg gagcggggga
    18961 aaagatggag attatatcaa attcttacct atcgctatag atgttaacga caaaagaaag
    19021 tgcggtgatt ttgaataagc ttaaacaaat cgtgatgtta ggccgtcaat ctggtttctt
    19081 tctgattcta gcttgtcaaa ggccggatgc gaaatatcta ggagatggga ttcgcgatca
    19141 gtttaacttc cgtgtggctt tgggtcgtat gagtgagctt gggtattcca tgatgtttgg
    19201 agaagtcgat aaaaattttt ttatgaaacg catcaaagga cgaggttatg tagatacagg
    19261 gggcagtgtg attagtgagt tttatacgcc acttgtacca aaagggtatg atttcttaag
    19321 ggaaattagc aacattgttg gattaaccgt tcatacggaa agagaaaata atagtgtgta
    19381 gcctaggaat atcaataatc ctgggctttt ttattgaggt gatttatgac atttttagat
    19441 ttattcgccg ggattggtgg ctttcgctta ggtatggaac aagccggtca ccagtgtatc
    19501 ggtttttgtg aaattgatga atttgctcgg aggagttata aagcaatcca tgatacaagg
    19561 aaggaggtgg aaatgcatga catcacaagt gtatcagacg aatttgttcg atccctcgga
    19621 ccagtggaca tcctttgcgg cggctttccg tgccaagctt tttcaattgc gggaaagcgg
    19681 caaggatttt ccgatactcg aggtacttta ttctttgaaa tcgctcggtt cgccactctt
    19741 ctccaaccta agtttctatt ccttgagaac gtccggggat tgctcaatca cgaaggaggg
    19801 gctacgttcg agacgatcct ccgaacgttg gatggattgg ggtatgatgt ggaatggcaa
    19861 gtgcttaact caaaggccta cgttccccaa agccgtgagc ggatcttcct tatcggacat
    19921 tctcgagatg catgtaccga acaagtattt cctatcattg gatcgtcttc aacatctgat
    19981 caaaacatca gaaacttggt aaatatcaac ccttccaatc gaggaatggg gggacaagtc
    20041 tatgggtcag atgacgtagc gcctaccttg accggtgatg aaggaatcaa aatcgcattg
    20101 ccggtaaagg atggaatttc tgtagcgggt atgttactgg gaaattttga acaaggcaat
    20161 cgggtttatg agataactgg aacagcgcct actttgtcaa cgaaacaagg cggaacgaag
    20221 atcatgcttc ggaaaaacga aacagaatat caagaggtga agccagggaa cagtgtgaac
    20281 ctggcttttc ctaattccac aagtcgtcga ggccgtctgg gaaaacaaag tgtgcatacc
    20341 ttattaacag gagaccaaca agcagtcgtc acggatcagt atcaaattcg aaagttaaca
    20401 cctagggagt gctggcggct tcagggcttt ccggactggg cttttgatcg agcttctcaa
    20461 gtcaattcag atagtcagtt gtataagcag gctggaaatt ctgtaacagt tccggtcatt
    20521 tttgatattg ctagaagatt aaaggaggta aaaacagatg atttatgatg agttgattgg
    20581 ggaatctact gggtgattgg caagattcag tcagatccgg aattggaaga agaactgtac
    20641 cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc ctgatgatcc gtatgtaatt
    20701 gatgaggaaa cggatgctcg aatggagctg aatcaagtca tcagtgaatt ggaacgaatt
    20761 gcggactttg ctaaagaacc tgatatccgc caatacttgt ttgagataaa agcagagttg
    20821 gaaattgagg gtattaccgc ggagtaacgg ccaaataaac cactgatagg cactgaccgc
    20881 gggggatttg cgaagcagaa atcctccgtg gtgctgtgcc tgtttatgga cggcgttagc
    20941 cggccatgat gtctcacccc ccgtatctaa caggggggta caaaaacaca aaaaataagc
    21001 aagcaacgat aaggaatggc tgtcctatca aggataataa gcaatttgtg tagatgttaa
    21061 ctgatttgaa aaagttaaca ccttaaattt ggaggtgaga acttggcaca aaggaattta
    21121 gattatcggc tattaaaaga ccggcgtaat gaatatggcg tttcacaaaa taaattagct
    21181 acgacttgtg gacttagtcg ccc
//
LOCUS       DACJGB010000053        20801 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.053, whole genome shotgun sequence.
ACCESSION   DACJGB010000053 DACJGB010000000
VERSION     DACJGB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.053"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..300
                     /locus_tag="IYI34_002006"
     CDS             <1..300
                     /locus_tag="IYI34_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR0559869.1"
                     /translation="DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKEN
                     RKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(724..894)
                     /locus_tag="IYI34_002007"
     CDS             complement(724..894)
                     /locus_tag="IYI34_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0559870.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1088..1267)
                     /locus_tag="IYI34_002008"
     CDS             complement(1088..1267)
                     /locus_tag="IYI34_002008"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559871.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1277..1501)
                     /locus_tag="IYI34_002009"
     CDS             complement(1277..1501)
                     /locus_tag="IYI34_002009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559872.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1654..2766
                     /locus_tag="IYI34_002010"
     CDS             1654..2766
                     /locus_tag="IYI34_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR0559873.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2844..3137)
                     /locus_tag="IYI34_002011"
     CDS             complement(2844..3137)
                     /locus_tag="IYI34_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAR0559874.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3305..3619
                     /locus_tag="IYI34_002012"
     CDS             3305..3619
                     /locus_tag="IYI34_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAR0559875.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3640..5619
                     /locus_tag="IYI34_002013"
     CDS             3640..5619
                     /locus_tag="IYI34_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAR0559876.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5632..5958
                     /locus_tag="IYI34_002014"
     CDS             5632..5958
                     /locus_tag="IYI34_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAR0559877.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5974..6492
                     /locus_tag="IYI34_002015"
     CDS             5974..6492
                     /locus_tag="IYI34_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559878.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6492..7844
                     /gene="celB"
                     /locus_tag="IYI34_002016"
     CDS             6492..7844
                     /gene="celB"
                     /locus_tag="IYI34_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAR0559879.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7928..9433)
                     /locus_tag="IYI34_002017"
     CDS             complement(7928..9433)
                     /locus_tag="IYI34_002017"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAR0559880.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9525..10115)
                     /locus_tag="IYI34_002018"
     CDS             complement(9525..10115)
                     /locus_tag="IYI34_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR0559881.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10294..11940
                     /locus_tag="IYI34_002019"
     CDS             10294..11940
                     /locus_tag="IYI34_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR0559882.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11989..12708
                     /locus_tag="IYI34_002020"
     CDS             11989..12708
                     /locus_tag="IYI34_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAR0559883.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13187..14134
                     /locus_tag="IYI34_002021"
     CDS             13187..14134
                     /locus_tag="IYI34_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0559884.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14146..15066
                     /locus_tag="IYI34_002022"
     CDS             14146..15066
                     /locus_tag="IYI34_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0559885.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15107..16564
                     /locus_tag="IYI34_002023"
     CDS             15107..16564
                     /locus_tag="IYI34_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAR0559886.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16909..17523
                     /locus_tag="IYI34_002024"
     CDS             16909..17523
                     /locus_tag="IYI34_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAR0559887.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17516..19240
                     /locus_tag="IYI34_002025"
     CDS             17516..19240
                     /locus_tag="IYI34_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR0559888.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19237..20688
                     /locus_tag="IYI34_002026"
     CDS             19237..20688
                     /locus_tag="IYI34_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0559889.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6709 a         3510 c         4166 g         6416 t
ORIGIN      
        1 gatggcttag caccaggagc atatcagctg gtagaaactc aagcgccaac ggggtatgaa
       61 ttggatgcga caccagtaac tttcaagatc gaaaaagaac aagaagtggc gatcttttta
      121 acaaaagaga atagaaaagt tgctgatagc tcagatataa gaggaaacaa tccaacaagc
      181 agtggaaata agcatcttcc aaaaactgga gaaaccatca ttagccagtt tatattgtca
      241 atattgggtg tgctacttgt tttcatcagt ataaaattta gaaagaaacg aaatatatga
      301 gctaaaataa aaaattgaat aagcatagtt tttaaaatca actagtaccc atcaattgaa
      361 tggaaactac cgattttaga aggggtatac aaacaaatat tagtatcgga gtagagaagg
      421 ttgaacccaa ccagtaaata agtaaagaca attttgtgct gcgttgagaa gtgaaaaata
      481 aacctttgta gtaatactag ttaataaaaa atatacaaaa acactgcgaa aatcattctt
      541 gagtttcgca gtgttttttg cctaatgact caagtttatt gggtaatact ttgattttct
      601 ttctacaaat aatcagcgat cagttgtaat tttttctcta gttttaatgg ctctttgtca
      661 ataaggagaa aaacaagtcg ggtcttttgt ctcggaaagc ttcaaccaac tcgtgaataa
      721 aggttacgcc tccttgaaag gcgtggaaaa accaagtaat cgatcaatca ccaatttcgc
      781 atttggtaga acatgtttgg tgagctgaaa gtaggtggcg ttcatgtctg taactaagaa
      841 ttctatttct ttaggattag tgcagctaag gaaatagttt gttaatcgag gaattttttg
      901 tgtaggtaaa ttttctcgat taatttttct gtctcgccat ccgcggaaat aaaactcagt
      961 ttatcctcaa cggaaacgtg agaacgaaat tcacaaccct caacactcaa gacagaattc
     1021 ttttggaaga ctttggtaaa tagcttctca actctttcaa tgtgcgaata acggttgtca
     1081 aagataccta acaacttttc gaaataaaaa ataacgacac tttttcagtc agtaaagaag
     1141 tgattttatg tctgacatga tttgaaatcg agtgccttgg acggataaaa taactttgag
     1201 cgttccaatg gcttcgacag tttttacagg tatagcgctg cttttttagg cgtataacca
     1261 ttggcatatg attatattat tcaaagtgga caatggtttc cttcttcttg tttttcacca
     1321 ccattaattt cctatttcca tctaccacag cagaaccaca atttctacag acacatggag
     1381 cagggaagag aactgcagca acgatcaatg tttttttctt ctgtaaggtt tcgtaagaga
     1441 cctctgtaat catcaaattt ttgtctattg atctcagaat ttttttgata gaaccattca
     1501 taaagcatat cgttctctca gttgtttatt ttgtggtgat ttaatcatac aagaaaacga
     1561 tatgtttttt tataaaaaca tgaataggaa aaatagttga caagaaaatg acaacgccat
     1621 atgatgttcc atagtattta caacctgtca tagatgactg taaaaaataa aagtaaaaag
     1681 aaaggacgac aacaggtgga tttttatcag tctctacaac tagatccgtt tattttaaaa
     1741 caaaaaatac gagaagcagc ttctaaaaaa gaaaagtgca tgtatatttg ttcattgttt
     1801 ttacgatcat tattcatcgt attgtttgcc atttgtttta tcattataat tacaacatta
     1861 tttgaatcga agcataaacc atacgctgtg gttttattct gtatgttgat gagtattcgt
     1921 tttgttgact ttggttataa gatttcccat tcgatcatag gtttagcaat cgtcatgttc
     1981 tcactattag tagcaccgta tgtccagctg atcaaatggt cagttatggg catgttgatt
     2041 cattttattt tactaagcag catattaatg gcaaccgcta gtgatcccaa aatggggaat
     2101 gcttctttgt acggcttttc atatctcttt attgtttatt cgttacctaa agattcgttg
     2161 aacaagaatt tctttacaca aacaagtagc ttattatttt tgtttttttg ttggttttca
     2221 gttctccttt acagaaaaca tcgagaaaag aacaaagaca aaagcttgtt taaagagatt
     2281 tttttgaaag gcatgtattc gcaggaaaaa atttggatgc tcatctatgc atttggcatt
     2341 agtcttttga tcgttgcagg agagtatgtc ccttttcagc gattgatgtg ggcagggttt
     2401 gcattttctt ctattgtatc tagctatgga ttgatgtcga ttggtttcaa agaacgagca
     2461 gtagaccgaa tcattggctc attgattgga tgtgttttat ttattggtat cagccaattt
     2521 attcctttta actgggtcgg tatattaggt ggtctagctt taggtgtttg ttctacctat
     2581 agatataaaa cagtttttaa ttcttttggt gctttggcaa tcactgccag tttattcggg
     2641 gttccaggag cagtaacgat ccgcattttt gagaatatcc ttggggtatg tttaggaatc
     2701 atgtatattg gggtgactga aatacttatc cgaaaaataa gagaaaaaca cggtttgaat
     2761 cattagatga gatcgaataa aaaataagga aaaagtgaag atacacatgg atgataaatt
     2821 gctatcattc catgtgtatc tttttacggt aaatcagtga aacgttcgac tttcattttt
     2881 aatttatact ttttacggag ttcgtttatt tgagccatca tttcggattt gtgatgcttt
     2941 tcgattgctt cttcatcttt ccaacggtcg atcagtaaaa gagattctgg atcttctagt
     3001 ggaaagtaat agtcatattt ctcgtttcct tcttctgcac gaatcgcttt gacaatgttt
     3061 ctttcgacca tttcttctgc aaattttctg gcttgtccat ttgtacttgt atacaaaata
     3121 ttcactgtga tactcactaa atcccctcct gaaaaaattc tatcactagt atatcacggt
     3181 gccagaaatt attattcaag atatgataca cattttttcc gcaatgctac ggaaatatat
     3241 attttgaata gaaaacgctt acaagatatg cttggtttat aaaaataaaa ggagtgattg
     3301 atttatgaaa aaagcattga ttatttgtgc ggcaggaatg tcatcctcga tgatggcctc
     3361 aaagacaacg gatttcttta agcaaaaaag agaagaggta ctcgtagatg cagtttcagc
     3421 tacagaaggc gataatatga ttaagaatag tgattttgat ttatttctga tcagcccgca
     3481 aacgacgatg tttttggata aattcaaaaa attaggagaa tctgttggaa agcctgttgt
     3541 tagtatccct ttccaagcgt atgtcccaat ccctacaggc atccagaagt tagcagaggt
     3601 gatcgaagag aacatatgat ggaataggag tggatgataa tgaaacagaa ggaaaaattg
     3661 ctgttgagat atttgattga tcacaagaag gagtatgtca ccagtcaacg tttagcttct
     3721 gaattgttat tatctgatcg gacaatccgt aattacttgc agcggatcaa agagttagta
     3781 gaaaaaaacg gaggtaagat cattgctaaa ccaggttatg gctaccaact gcatatttta
     3841 caaagactga catttgactt gtttttgagt cggcaagaaa tagtacctgg atatgctaga
     3901 gaaccgcaag gattttatga gtccgaagat cgacaaaagt atatattgaa ccaattgctt
     3961 ttggaagatg ccattctttt tatggatgat ttggcagaag agctttatat cagtcgttcc
     4021 agtttgacaa aggacatgca agaaatcaag gagcgattga ttccatattc actaaaaatc
     4081 gtttctaagc atggtcaagg gacatggatc gaaggggaag aaagaaatcg acggcatttt
     4141 atcatggata ctttttttgg aaaccattat ggaaattcat tgaaagaata tttagggcaa
     4201 agccgttttt tcccagacat cagttttgaa gagctagtca ttatcattct cgatgaaaca
     4261 agagaagcta agttaaaagt ttcggatttt atcatacaga atctgacttt acacttggct
     4321 ttggggatca aacggctccg agaagggttt gaaatcaagg aattaggcat cgaacaagaa
     4381 gtttctaagc gagtagagta tcaagtggcg cagcggatcg ttcaacgtat tgaggtaatc
     4441 gcaggtgtat cgtttcccga agaagaaatt gcttatttga cattacatct catggctaag
     4501 tcgaatcaca aagaaaatca aaatgatcag gaactatcaa aggaattagc gaaagttctg
     4561 aaaagattgt ctcaggaatt tcatttttca ttagtagagg atctccagct tttcaatgga
     4621 ctactcgatc atttgtcacc gatgatgatt cgattgagcc gaggtattcg cattgagaac
     4681 ccattgactg aggagataaa gaaagaaaat cccaaagtct ttgacgcagt aaaaaggcat
     4741 ttcagcaaca tgccggcttt gaaaaactat accatcaatg aagatgaatg ggcctatttg
     4801 gctttgcatc tcatggcggc attggaaaaa gaaagagctg cgcataaact acatgcattg
     4861 attatctgtg caacgggtta cggcagtgcc caactattaa agaatcgcgt agtcagtgaa
     4921 ttcggtaaaa atattacagt ggtaagtgta aaaggctatt atgagatcaa tgaaacaact
     4981 ttaaaaggaa tcgacctgat tatttcttct attgatcttt cgacaatgtt tttcaaaatt
     5041 ccagttttac atgtcagtat ttttttgaat gaagatgatg ttcgtaaaat acgaaaggta
     5101 gttggagaat ctatacccta tcattcctca gatagaccga taccagcatt ttctctacag
     5161 caaaaaaagc agatttacac cgaacagttg tctgaaaaat ttttcaagac ttatacgtac
     5221 gaaccgaaaa aggaagaaat tcttcatgat ttgctgcaaa agctgagcat aaatgaagaa
     5281 gaaggatata taagagaaat gcgaaagcag atccagcaac gtgaaaatat gggacaaatc
     5341 atattcagtg atacagtggc agtcccacat cccgctgttc cagttggaat cgaaacaaag
     5401 gtagccgttg cattgattcc ttctgggatg cagtgggatc aatatcaaga aattcatttt
     5461 gtttttttag tttcgccttc ttatgtcgaa aatgaaggaa ttacggtcgt gactaaagca
     5521 atcgttaggt tagttgacca gctaaaagta cagcaggaaa tcttagctga acctattttt
     5581 gagaatttca gcagtaaatt tatcaaactg atcgaataag gaggaaaaca gatggctgaa
     5641 aaaataaaca gtgaaacact acaagtgact tcttttgata tcattttcca tagcggaaat
     5701 gcacggacac tgatacatga agcatttcgc tcgatgcgta aagagatgtt tgaagaagta
     5761 gaagagaagt tgcgacaagc aaatgaagag attcttcatg cgcatcagtc acaaactcaa
     5821 cttctgaaag aatatgccag cgggacaaaa atagaaatgg aaattatcat ggtccatgca
     5881 caagatcatc tgatgacgac gatgacatta ttggaagttg cacaggaaat gaattatttg
     5941 tatcaaaaaa ataaatagaa aggaaggatc attgtgcagg atacagtaaa aaggaaaatc
     6001 gaattagaaa aaaagcaact tagtttgaaa aacatgtatt tcaatcgtta tctaatcgta
     6061 cgttacctga ctgccttttt ctttttcatg aacatgcaat ggatgattct gcttctatca
     6121 gctaaaagtt taggaagtag tttaccgatg attctgattt tagctatttt gcctgcagta
     6181 ggagaacaag taaagcttta tcgaaaacac caaacgaacg ttccatggac aaagtggtat
     6241 tttttgttcc agagtgtctt taatttatta gtgattccag tgttgtttac ctcaggattt
     6301 acattcttgt atccttttat ggcaaataac aatggaggac agttatttgt tttcattctt
     6361 gttgtaagcg gtatttttgc cagtgcattt atacagtatc gtttaaagaa aatttcactc
     6421 aaccaagacc aacagtatat ccgtattaaa caatatgaaa aagcgttata tttaggaaag
     6481 gagaataact aatgtttgat tttttgcaga aatacctgat gggacctatg gggaaaattg
     6541 cgcaacttaa aatcgttcgg gctgtcatgg cagcggggat ggcttctatt cctttcacga
     6601 ttgtaggctc gatgtttttg gttttaaata ttttgccatt accgttccca tttttagaag
     6661 gattttttaa tgcaactttt ttcaaagtta gtgacttgta catgatcgtc aatacaatga
     6721 cgatggggat tttatctatt tactttgcca ttgtgtttgc ttatgaatta acgtcaattg
     6781 aacgagatga acaaaaatta aatgttaatc cgctcacggg ggcattacta tctgtttttg
     6841 cattctttat gtgtatccct gaattgatca tgactgatgg taaaatcagc ttgattgctt
     6901 ctatcaatga gacagaaact gtagtaaatg gtattaggat gggctctttt gtcgaacgtt
     6961 taggaacttc aggaatcttt acagctatta ttatgtcgat tattgcggtc gaattgtatt
     7021 gtatgtgtgt caagagaaat tgggtcataa aaatgccaga tgttgttccg ccaggagttt
     7081 ctcgttcttt cactgctctg atcccaacat ttgtcattgc gtttgtggtg atgatcatca
     7141 atggattatt agttgcacta ggaacggata tcttcaaaat gattgctatc ccatttgggt
     7201 ttgttacgca gttgacgaat acatggcttg gtgtcatggt gatttatttc ttgatccatg
     7261 cattgtggat cgtagggatc catggagcga atatcatcac ttctttcttg accccgatcg
     7321 tattagctaa tatggcagct aacgcacaag gcgcaaatta tccgttggca ggagaattca
     7381 acaattctta tgtaaccgtt ggtggatcag gtgcgacttt aggattgatc atctttattg
     7441 cattcctcgc gaaatctgat cagctgaaag tgttaggaaa agcctcactt gtaccaggat
     7501 tattcaatat caatgaacca atcattttcg ggatgccaat cgtctacaat ccctatttag
     7561 cggttccgtt tttcttagct ccgatggctt ctgctacact cgcttatttt gccattcggc
     7621 tggaaatcgt caaaccgatg cttgcacaga tgccgtggcc ttcaccagtc gggatcggag
     7681 catttgttgg tagtggtggt gattggaaag ctgctgtgtt agcagtcatt tgtgcaatca
     7741 tcgcattcat tatctggttc ccatttatca aattctatga taataaatta ttaaatgaag
     7801 aaagagaaaa ggcagcagaa ttagctagtg aaaagttaaa atgatttcta agaaaaaagg
     7861 aactgtgaca aaaaatcaca gtcccttttt ttcttagaaa tgggaaacca ctagtatata
     7921 aataccatta ttcttcaatc aaccgcttca cgattgcata atccccttca taaggttcat
     7981 ctacaccatt gattttttga tagggatcca cgccttttcc cgcaataacg actgaatctt
     8041 gcggagcgct ttctgctaaa gcgcgtcgaa tcgcttcagg acgatccatt tcataatgaa
     8101 cgatcaaatc tggattagta attgcttcat tgatttcttc cgcgattttt ttcggatctt
     8161 caaatgctgg gtcatctgcc gtcaaaaagg cgatgtcagc tgtttctgat agcacttttc
     8221 caaaatcatg tcgacgggag attgctttgt ttcctgggct gcctaataca acaattacac
     8281 ggccatctgg atgttcattt ttggcaaatt tcagcaatgt tttcaagctt agataattat
     8341 gtgcatagtc cacatagaca tgcgctccat tggattggat caattgatcc attcggcctg
     8401 gtacacgagc ttcttttaat ccgtcttgtg catcttgact ggaggcacct gttagtgctg
     8461 cggcaatgat cgcactggct gcattttctt ggttgaatcc tccagccagc aaaatatcat
     8521 aagcagtatc gcagatagct aagcgatctt catgagaaga taaagtaaaa ccgtgagtac
     8581 ctttatctga acggatcact tgataattgc tttcttccga acgaccataa gtgatcgttg
     8641 gaatctggaa tagatcggct gtttccctaa gtaaatcaaa atagtcactt tcatgattta
     8701 aaacaactgt ttttgaattc aagatcaatt gtcgcttgca atagaaatag tcatcaaatg
     8761 tcggatgttc gatcggactg atatggtcag gcgagatatt caagaagatt ccgacatcta
     8821 aagtcaagcc gtatacgcgt tgtgttttgt aagcttgtga agaaacttcc atcactagat
     8881 gagtcatacc attttcaact gcttctgaca tcatacgata taaatctaat gactcaggcg
     8941 ttgtcaaatg ggatttgaaa tatgttttgc catctaacgt tgtattcata gtagaaagca
     9001 atgctgtttt tttatttgtt gtgtgatcca agattgcttt tgtaaagtac gcagcagttg
     9061 ttttaccttt tgttccagtg aatccgatca ctttcaattt attttgcgga tagtcgtaaa
     9121 aagccatagc aagaagcgcc attgctttac ggatatccgt tacgatgatt cctactgctg
     9181 aattttcgta tggttgttct gatacataat attgaatacc ctgttcgatt gctgaatcca
     9241 aatattcttc tttgaaattc attcctttac agaaaaacaa agtagaagca tcagcttttc
     9301 ttgaatcata acttaactga agaaattcag ctttttctgg tacatcaaga tgccagcctc
     9361 ttgttgaaac aaattctttt aatagatttt cttttaatag taaatgacgg atgtcttcta
     9421 aagagaagct cattgattga tccctcgctt tattcaattt cttggtttat tatagcacac
     9481 tctttttaat atatcgtttg tttccgctat gacaaaatcc agctttattc aaattttctt
     9541 gacacagggg acaagcggat gagtattttc ataaacaatg aactgactgt aatagatagg
     9601 agaacgaata gtgtctccaa caatccaaaa acataataat tcaaaaagac gataagacta
     9661 ttggtaagta aaagtccttg cggcattgga agccgaaaaa atttgtggaa aataatcggc
     9721 ggtatcgtta taccgccatt tgaggccccg actgtgtaca atgtgtaaac cccaagactg
     9781 aacaaaagac ttcctgaaat cagtgaaaag aaatgtgagg agatcatttc cacttttgga
     9841 acaatctcta aaacaacagg aaaaagcaga cttcccgcta cagtcttaaa aaatactgat
     9901 tttcctaata ggacagcggc tagaagcagc atcataagat tgatgctcca aacagtaact
     9961 gagcgttgga tagaaaaggc cgtttcaact aaatgtccga tggcgcccac tccggcggaa
    10021 gctatatcgt gagggagcaa gaaccaattc aatccaactg tgataaagaa aagacctaaa
    10081 aatataacgg gtagtttttt tatttcttgt gtcattgata tctcctataa atgttagcca
    10141 taaaaggctg tatatttctt tactttacca aaaaaacgaa accgaattca aaaaaacaat
    10201 tgttcttata aaagttatat aaaatcgatt gccagtttct gttttaccaa ttaaaatgat
    10261 agtaatgttt tgtagaaagt tggaatctga ccgatggata atcaaagaaa accagcatta
    10321 gatcaattaa caccgcaaga aaaaatggca cgaggctctg cttggatgac cgccagtaat
    10381 atcatttcaa gattgctagg cgctgtctat atcattccct ggtatgcttg gatgggcgaa
    10441 aatgctaagg ctgccaatgg attattcaat atgggataca atatatatgc attgtttcta
    10501 ctgatctcta cagcaggaat acctgctgcg attgcaaaac aaactgcacg ctataattca
    10561 ttgaatgaat atggcacttc acgacgatta tttatccgtg cattacagat gatgggcggg
    10621 ttaggcctgt tgtttgcttt gttcatgtac atcgcctctc cttggctagc tcatgcttct
    10681 ggtggcggag aagaactgat tccaacgatg cgttctttga gtatcgctgt attggttttt
    10741 ccttgtatga gtgtgatacg agggtatttg caaggcaatc aagaaatgat gccttatgcg
    10801 ctgtcgcaga ttgtagaaca ggtggcacgt attttttata tgctattggc tacattcatc
    10861 atcatgaaag tcatggatgg tgactatgtg acggcggtca cgcaatcaac tttcgcggca
    10921 tttatcggta tgctagcaag tattcttgtc cttttgtatt ttttgaagaa acatcaagcg
    10981 tatacatcgg cgtttattca ctacagcgaa aacaaaataa atattgcaac gaaggaattg
    11041 ttgttagata ccgttaaaga agcgattccg tttattattg tgggttcagg ggtaacgatc
    11101 ttcaaattag tcgatcagtt cacttttatg aagatcatgt ctgcatccac cgagtattcc
    11161 aatgcacagt tactggatct tttttcgatt tttagtgcca atccagataa gttgacgatg
    11221 gtagttattg cattggcaac atcgatcgcg tctactgggt taccgttgat tacagaagct
    11281 gttacgttga aagatcgagt aggtctggca aaattgacaa gcggaaattt acagttgttt
    11341 tcatttttta tgtttccggc gacatttggt gttatgttac ttgcctatcc gctaaataca
    11401 ttattttata caccggattc tctaggaagt aatgtactga tccaagcaag ttttgtagga
    11461 ctcttcctag gactatatat gctagtttcc aacatgctgc aaggaatgtt tgaaaacaag
    11521 gcggcaatta attatttggt tattgggttt ctagtgaaac tccttcttca atacccagcc
    11581 attcgtttat ttgaagtata cggcccttta ttggcgacaa tgatcggatt tgctgtgtct
    11641 tgtaccttga tcttaaagag aatccatgaa gtagcccgct ttaaaccaag ttttgtctgg
    11701 cgcaggacat tgctgatctt tattcttacc ttgattatgc ttttggcttc gtggatcaca
    11761 aagatgattt ttggtgcttt tttaaacgaa gatagcaaat tccagtctct attgttgatt
    11821 cttgtagtag caggtatagg ggctttagtg tatggttatt tagctttgaa aatccgcttg
    11881 gcagaaagac tactaggacc aggaatagcg cgtttacgac ataaattaaa aataaaataa
    11941 gtgaatcaga agagctagat tgatgctctt ttgaaatggg ggaaacgcat gcgtttagat
    12001 aagtttttag cagatatggg acttggcagc cgcaaagaag tcaaaggatt aatcaaaaaa
    12061 ggaacgatcg aagtcaatgg acaagtggtg aaaagcgata aagcgcaagt aaatgaattc
    12121 gaagatgcgg ttacttattt aggagaaatc attacgtatc aaaaagattt ttactacatt
    12181 ttgcataaac ctgcaggagt catttctgct actcaggata atcatgatca aaccgtaatc
    12241 gatttattgg aagaccagga ttatcgggaa gatctttttc cagtcggccg attagataaa
    12301 gacacagagg gcctcttgat tttaacgaat gacggggtgt ttgcccatca gttactatca
    12361 cctaaaaaac atgtggagaa agaatatcta gctgaggtta gcggtataat gacagaagaa
    12421 gatatccgtt tatttgcaga aggactgatt attgatggcg atgagcagac attgccggca
    12481 aaactccaaa ttgatcaagt agatcaagag acagaaactt caattatccg tctgattttg
    12541 catgaaggaa agtttcacca agttaagcga atggtcaaag cagtaggaaa agaagtctta
    12601 tatttaaaaa gaatccgtat gggaaaattt tggctgccta aagatttgaa taaagggcaa
    12661 tatcgaccca tgaccaaaga agagctagaa caagtgagag aaaaataatt gaatggatag
    12721 ctaaaagaat aagcagagta gttgtagcgc catctgaaaa gcagacttgt ccgtctgact
    12781 ttcggatggc ttttttgtga aatttcggct tatttccgta gaagctactt ctggaacacc
    12841 gtttgttcgg aataagggac tgtgacaaga cttttgttac agtccccatt gttccttgtg
    12901 actcagagtt agatagtaag gacacgatct ccctaatact caggccaaaa cgctgaattc
    12961 acaacctctg ataaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    13021 aaatatgagg aactattttc ccgttttttt ctctaatact caggccaaaa cgctgatttc
    13081 acaacctctc ctatactttg tttcattttc cctatacttt gtttatttat tctcattatt
    13141 gaaagcgcta taattacgtt gcgaatgaat cgaaaggggt ttttagatga aaaaacgaaa
    13201 ggggttcaat cattttttta tgaatctatg gcgttataaa gcacttgtcc tgatggctgt
    13261 accgggtatg ctttggatga tttttttctt ttacatacct gtaatggcga atgtcgtagc
    13321 attcaaaaat tttcatattt cagcagatgg gttcttggct agcttgaggg aaagtccttg
    13381 ggtgggttgg gaaaatttca aatttttatt ttcttcggat caagcatttc tcatcactaa
    13441 aaatacaatt ttatataatg tagcatttat ccttttgaat ctgctgatct cagtcgtatt
    13501 tgcaatcatt atgagcgagt tgagaaataa acgactcgtt aaagtttatc aaacgatgtc
    13561 cttactacct tattttctat cttgggtcat tatcagttac tttgtttacg cttttttaag
    13621 tcctgacaaa ggaatattca atcagtggat cactggacat ggaggagaag ctgtcaattg
    13681 gtataatgaa ccgaaatatt ggccgtttat cttggtgttc attggtacat ggaaagggat
    13741 tggttataac agcattattt actttgcatc tgttatggga atcgatccga cttattatga
    13801 agctgctatg gttgatggag ctagcaaatg gcagcagatc aaacatgtga cgataccaca
    13861 acttattcca ctaatgacga ttttaacgat tttagccgta ggaaacattt tccgagctga
    13921 ttttggcctg ttttataaca ttcctaggaa ttccggtgca ttatatgatg tgactcaagt
    13981 tttggatacg tatatctaca atggcctgac atccacagga gattttggta tgacggctgc
    14041 tgcaggactt taccaatcag ttgtcggctt tgtcttgttg atgatcacaa atacgatcgc
    14101 ccgacgtttt gatagcgatt ctgcattatt ctaaggagga aaaaaatgaa aaaaaagaaa
    14161 gtggagagag tccaagtccg ctctttcaat aagacggcta attttttctc taatataatg
    14221 attgcactct ttgctatttc ctgtattttg ccatttatat ttgttatcgc catctctttt
    14281 accgatgagt ccatattgtc gactcacggg tacagttttt ggccagaaaa gttcagtata
    14341 tttggttata cgtacttgtt cgaccagatg caggataaaa ttttccaagc gcttttcgtc
    14401 actgtacttg ttactgtttt aggtacactg atcaatagca cggctacttc tttatatgct
    14461 tatgcgattt cacggtcaaa ctttccattc cgtcgttttt ttaccgtttt ttgtttgatt
    14521 acgatgttgt tctcaccagg gatggtggcc aattatcttg ttatgacaaa tcttcttcag
    14581 ttaaaagata cgatttgggc attgatcttg ccaatggctg tcagtccctt caatattatt
    14641 gtgatgcgca cattctttaa acgttcggtc tctgattcga ttattgaatc ggcacggatc
    14701 gatggagcta gcgaactgcg aatatttgtt cagatcgttt taccgttagc tgttccaggt
    14761 attgcgacaa tcagtttatt tgccgcattg ggttactgga atgactggtt caatgcttta
    14821 ctatacatcc aagatgataa gctagttcca ctacagtatc ttttgatgaa aatccaaagt
    14881 aatattcaat atctgacaca aaattcagga gcaggaagcc aattgacagg cggattagcc
    14941 tcagttccag gagaatctgc cagaatggcg atcgtagtca tctcgacgtt gcctattgcg
    15001 atcagctatc catttttcca gaagcatttt gtcaaaggtc taacaatcgg cggagtaaaa
    15061 gaataaagaa acaaaaatat aaatcataaa attaggagga agaaaaatga agttatggaa
    15121 gaaactagcc tttacaggtg tttcagcgtt gttgttggga acattagcag catgcggagg
    15181 ttctggttct aaagaccagg cagaagctag caattcggac actttgcaga tgtatcaaat
    15241 cggagataaa ccagataatt tcgatcaatt gatggaagta gcaaacaaac gaatcgaaaa
    15301 agagatcggt gtaaaagtca atatcaatta cattggctgg ggcgattacg aaaagaaaat
    15361 gaacgtcatc atttcttccg gggaaaatta tgatatcgcc tttgcgaaca actatgtttc
    15421 caatgcccaa aaaggagcct ttgctgattt gactgaatta gcgcctaagt acgctgaaaa
    15481 agcttataat gatttagatg aagcatatat taaaggaaat ctggtcaatg gaaaattata
    15541 tgctttccca gtcaatggaa atgtctttgc tcaacaagta cttactttta acaaagcttt
    15601 actagataaa tacgatcttt caattgatgg aatccaatct tacgctgatg cagaaaaagt
    15661 cttgaaggaa ttccatgaaa aagaaccaaa cacggcagct tttgccattg gacaaggctt
    15721 caaagtccaa gggaactttg attatccttt agggaatacg ttaccatttg ctattgatct
    15781 ggatggagat gattcgaaaa tcatcaacca atacgataat gaaacgtata tcggactgtt
    15841 acgtacaatg catagctggt acaaacaagg actcattcca agtgacgctg caacgagcaa
    15901 taaggattat ccactagaag gaaatacttg gctaatgcgt ggagaaacac aaggaccgta
    15961 tgattatggc gatacaattt tgacaaatgc agcaaaacaa gaacttgtct caaaagcaat
    16021 tactgttcca ctaaaatcta cagctcaagc acaaatggca aattttgtcg tatcaaatac
    16081 ttcaaaaaat aaagaaaaat cagtagaact tctagggctg cttaacagtg atccagaatt
    16141 attgaatggg cttgtttggg ggatcgaggg agaagcatgg gaaaaagtcg atggttcaga
    16201 tgataaaatc aaattgctag atggttatca gccaaataca catatgtctg cttggaatac
    16261 aggaaacaac aagattctat atacacaaga gtctattacg gatgacatga ttgccaaacg
    16321 tgatcaatcg attgctgatg cagaaacttc accgatttta ggattcagct tcaacactga
    16381 cagtgtcaaa acagaattaa gtaatatttc taatgtcatg aatcaatatt tagatggatt
    16441 gaatacaggg actgttgatc cagatgaaac attaccgaaa ttgaaagacg ctttggacaa
    16501 ggctggctat gataaggtat tgaaagaaat gcaaaaacaa tatgatgagt tccgacaaga
    16561 ataataatga ataaagacaa aggggttgtg acaaaagacg tgtcacaacc tcttgttccg
    16621 aataaacggt gttcaaaagc cagtttctcg gtattaagct aaatatatgt aaaaatgtgg
    16681 aaagccatat tcacaaaatt tttcttacta ctcgaaacta agcggctatt tcacaaactc
    16741 ttggtaaacg gtgttcaatc agctaaccct cggtattcgc tcacaaaacg acaaaatatg
    16801 aggaactatt tttccgtttg tttctctaat aatcaggtca aggtgctgat tgcccaacct
    16861 ttttttgtat aatgagaaag tataaactta atgtttggag gagaagatat gttagggaag
    16921 gttttggaag agttatttat ccggatctgg gtcattataa aactaacctt gtatttctgg
    16981 atatatacat ttgccggcgg gatcatcttt ggattaggtg cggcatggaa aacagtcaat
    17041 gaactatttt atttgtacgg attcgagtat aaagaaatca caataaaaag aggctggaat
    17101 atatataaaa gaaatttttt aagaggcaat ctgctattta gtttattttt atctggtact
    17161 gctttattga gttacaattt atatttatct gttcaaatca aagggctcat ttttttagct
    17221 attgatttta ttttactttt tgccttgctc tgcctatttg ctacttatca atacagtatg
    17281 attttagata gtgaatatac gatctctgtt cctaatctgc taaagctaag ttttatttcg
    17341 gttttttcca gcttttcgtc ttttctaaaa ataattattg gtagcggtat aattttaggc
    17401 gtgacttggc aattcaaagg attgatcctg tttggtgtca ttggtctttt gacggtttgg
    17461 aatggtacga tgacaacaca ttggcgtgaa gaattagata aacagttgga aagttatgaa
    17521 taagtttcgg attctatttt caaaaggaac cttactaaat cgtctgttgc ggaaatacgc
    17581 ggttatcatg atcggtgtaa cgatgactgc taccgttatt tttagtatcc atacttggga
    17641 acaaaatcaa aaacaggcag aaaacatgac aagtgatgcc gttcaaagca caagcagaat
    17701 gctgaatgat aaaacaacct tgagtaggat catcaaaaac cagcttgtag ggaattccga
    17761 aaagatcgaa aatgtgacga cctacttaac aaaacccata gatcagtatc tgatgtatgt
    17821 atacgaacag caaaacagta ccgatgaatt agtttctttc ccaaatcaaa tcaaagatct
    17881 ctacgcaaat tatgaagaac taagtgcgat atatatcgtg ttgaatcaac taccggagta
    17941 ttatgaatct tctagagaaa acaaaggtgg ggaaataaaa aaggggacac caaaattact
    18001 agacgccttt tatatcaagt tgcctatcac acaaggagga gctgagtcag gaagtatttt
    18061 tattggtttt caaaagaaag aactagatat gattttagaa aatctaacgt cattcaatgg
    18121 actctcttta tacatgattt ctggaacgac aaatcgacta tacacattcc atgataataa
    18181 aatagctaaa gaaacgtttg aaagacaaga aatgatcatc acagagagtc tgaagaaaaa
    18241 ttcacaattg ccgattgacg atctggaaaa aaacaatttt gttcaacacc aagaactgtc
    18301 tgatgattat cgtatattag ctgtactaga taaaaaaagt gtaaaaatcg aaacgggaaa
    18361 aaatcttgcc ccgctgatca ttggtatcat tattttagac aatatactgc ttatctttct
    18421 ttataaaaca ttcaaacgat attcacagca agtatctatc gtcatgcagg ccatggatca
    18481 aacagcagca gggaatttag agacgcggat cgacataaca aacacggaat atgaactgaa
    18541 ggaactttca atcggtatca acgaaatgtt ggatagcatc aatcagtttg tagaagacat
    18601 ctacaaatta gaaatcaaac agcaagacgc acatatgcgg gcgcttcaag cacagatcaa
    18661 tccacacttt ttatataata cattagaata tatccgaatg tatgcaatca gcgaaggaag
    18721 tgaagaatta gcagatgttg tgtatgcgtt ttccgctttg cttcgaaaca atacgaatca
    18781 agagaaaacg atcactttaa aagaagaact ggacttttgc gaaaaatatg tctatttgta
    18841 tcaaatgcgt tatcctaatc gagtagccta tcattttatg atcgatcctg atttggaaaa
    18901 gatcgaagta cctaaatttg tgatccagcc attagttgaa aattatttta aacacggtat
    18961 tgattttact cgattcgata atgcgctaag tgtcaaagtc ttgcaggaag gcaaacgggt
    19021 aaggatcatc atcaaagata atggaaaagg gatgactgaa aaacggctaa aacagattga
    19081 agaaaaactg tctcatccta aagtggaact tcacggttct atcggtctgc aaaacgtgaa
    19141 tgaacgttta cgtgccagct tcggcagcag ttattatatg tcgcttgaga ataatgagac
    19201 aggtggactg accgtttcta ttacattcaa ggagggatga gcgtgtatca agtgttattg
    19261 gtagatgatg aatacatgat tttgaatggg ttaaaaaaga taattgattg gcaaagttta
    19321 ggctttcaaa tagtagcaac agcagagaat gccaaagaag gactagctgt acttgaacag
    19381 cgacagattg atttagtagt aacggatgtg acaatgccag aaataaatgg actggaattt
    19441 attgaagcgg ctcaaaaaga acgtcataat tttgaattta tgattttatc aggataccaa
    19501 gaattcgatt atttgaaagg aggtatgcaa ttaggcgccg tgaattattt gatgaaaccc
    19561 gtgaataagt tcgaattggt tgaaagcctc aagaaaatca aaacacggtt agatcagcaa
    19621 aatgaacaaa aaaatcaaca agaaatctat caagaactgt tattcagtca atggttgaat
    19681 gaagaattgg atgaaataag tgaagaagaa ataataggac aaataagtga gaagcagcat
    19741 aggatagtat tgattcaact ggcaagaact catgatactc ttatcaatag atggttgcaa
    19801 gaacggcaac aaatctttta ctatcaacga aattacggag accgtgtatt gatcacgttg
    19861 ttgttggagg cagatgaagt tgatggcttc tgttgttttg ttcaagaaaa ctttcctgat
    19921 caggaatggc tgatcagtat cggggaagag acagattctt tggacaaaat ccctgaaaca
    19981 ttccaacaag cgaaagacaa tctacagttg catcaatttt atggagataa agacgagcat
    20041 gttttttacg cagtacaagc agaaacaaaa gatcaaacca tcgatttttc aagtttcaga
    20101 cgcgtgctgc aaaataaaca attggaagtc gctcaaaaaa tgatcactga tttttttgaa
    20161 cagtttcagt tagcagccat gatgccggaa gatatacgat attccagttt tttactgttt
    20221 atggagattc aacgggaatt gattgatctg gaagatgagg aatatttaca aggaatcgaa
    20281 aaaatccatc aggcaaaaac ggtgcaagaa cttcagcagc tacttctgtc attcatcaaa
    20341 aagcatcaga gacagaaaaa gtacagtgac aatgtggaaa aagtaatcgt aatcctgcac
    20401 cagcattatc aagaaccact tacactcaaa gaagtgtcag aaaacctgca tttaaatgtc
    20461 atgtatctag gacagctgtt caaaaaagaa acgaaaaaaa gtttttcagc ttatttgaac
    20521 catttacgta tggaaaaagc gaaacaactg cttttgcaca gtaatcaaaa catcaatgag
    20581 atagcaagtg agattggcta caacaacacc acgtatttct ctaaattatt caagaaaatc
    20641 gtcggcagat cccctaaaga gtaccgagaa aacagtggag aatcataaaa gaaaaagcca
    20701 atgtaatgaa ataacttata aaaaattgtc tagaaatcta attttgggct gttcttatta
    20761 cttgaggcaa ggcagctttt tcatgacctc tcgataaacg g
//
LOCUS       DACJGB010000054        20669 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.054, whole genome shotgun sequence.
ACCESSION   DACJGB010000054 DACJGB010000000
VERSION     DACJGB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.054"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            242..2713
                     /locus_tag="IYI34_002027"
     CDS             242..2713
                     /locus_tag="IYI34_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR0559890.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2816..4552)
                     /locus_tag="IYI34_002028"
     CDS             complement(2816..4552)
                     /locus_tag="IYI34_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAR0559891.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4549..5253)
                     /locus_tag="IYI34_002029"
     CDS             complement(4549..5253)
                     /locus_tag="IYI34_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0559892.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5384..5887
                     /locus_tag="IYI34_002030"
     CDS             5384..5887
                     /locus_tag="IYI34_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAR0559893.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5847..6185
                     /locus_tag="IYI34_002031"
     CDS             5847..6185
                     /locus_tag="IYI34_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAR0559894.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6206..7624
                     /gene="ffh"
                     /locus_tag="IYI34_002032"
     CDS             6206..7624
                     /gene="ffh"
                     /locus_tag="IYI34_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAR0559895.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7736..8314
                     /locus_tag="IYI34_002033"
     CDS             7736..8314
                     /locus_tag="IYI34_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAR0559896.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8318..8839
                     /locus_tag="IYI34_002034"
     CDS             8318..8839
                     /locus_tag="IYI34_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR0559897.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8918..9472)
                     /locus_tag="IYI34_002035"
     CDS             complement(8918..9472)
                     /locus_tag="IYI34_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAR0559898.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9725..10000
                     /gene="rpsP"
                     /locus_tag="IYI34_002036"
     CDS             9725..10000
                     /gene="rpsP"
                     /locus_tag="IYI34_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAR0559899.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10012..10263
                     /locus_tag="IYI34_002037"
     CDS             10012..10263
                     /locus_tag="IYI34_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAR0559900.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10388..11179
                     /locus_tag="IYI34_002038"
     CDS             10388..11179
                     /locus_tag="IYI34_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559901.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11349..11870
                     /gene="rimM"
                     /locus_tag="IYI34_002039"
     CDS             11349..11870
                     /gene="rimM"
                     /locus_tag="IYI34_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAR0559902.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11860..12621
                     /gene="trmD"
                     /locus_tag="IYI34_002040"
     CDS             11860..12621
                     /gene="trmD"
                     /locus_tag="IYI34_002040"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAR0559903.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12757..13104
                     /gene="rplS"
                     /locus_tag="IYI34_002041"
     CDS             12757..13104
                     /gene="rplS"
                     /locus_tag="IYI34_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAR0559904.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            13477..14139
                     /locus_tag="IYI34_002042"
     CDS             13477..14139
                     /locus_tag="IYI34_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559905.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14218..17445
                     /gene="fss3"
                     /locus_tag="IYI34_002043"
     CDS             14218..17445
                     /gene="fss3"
                     /locus_tag="IYI34_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAR0559906.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            17559..17873
                     /locus_tag="IYI34_002044"
     CDS             17559..17873
                     /locus_tag="IYI34_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR0559907.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            17886..18260
                     /locus_tag="IYI34_002045"
     CDS             17886..18260
                     /locus_tag="IYI34_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAR0559908.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18270..18614
                     /locus_tag="IYI34_002046"
     CDS             18270..18614
                     /locus_tag="IYI34_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAR0559909.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            18685..20037
                     /locus_tag="IYI34_002047"
     CDS             18685..20037
                     /locus_tag="IYI34_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAR0559910.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20097..>20669
                     /locus_tag="IYI34_002048"
     CDS             20097..>20669
                     /locus_tag="IYI34_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAR0559911.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNL"
BASE COUNT         6909 a         3689 c         4366 g         5705 t
ORIGIN      
        1 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
       61 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc
      121 tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt
      181 tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca
      241 catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga
      301 taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga
      361 acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca
      421 aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt
      481 gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac
      541 aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt
      601 aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga
      661 ccttgtaata gatgacattg ttattttaga gactggatat caaattccag cagatgcaga
      721 ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat
      781 aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta
      841 cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa
      901 gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg
      961 gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa
     1021 tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc
     1081 tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa
     1141 gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct
     1201 ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt
     1261 ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta
     1321 tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa
     1381 aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag
     1441 tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca
     1501 attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt
     1561 ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt
     1621 aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt
     1681 caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa
     1741 tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc
     1801 agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga
     1861 acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga
     1921 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     1981 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     2041 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     2101 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     2161 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     2221 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     2281 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     2341 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     2401 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     2461 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     2521 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     2581 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     2641 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa
     2701 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     2761 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     2821 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     2881 taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     2941 aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     3001 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     3061 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca attagatttt
     3121 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     3181 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     3241 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     3301 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     3361 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     3421 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     3481 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     3541 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     3601 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     3661 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     3721 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     3781 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat
     3841 acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat
     3901 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     3961 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     4021 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     4081 gtaaaaaacc gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     4141 tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc
     4201 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     4261 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     4321 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     4381 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     4441 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     4501 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg
     4561 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc
     4621 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     4681 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     4741 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     4801 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     4861 ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg
     4921 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     4981 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     5041 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     5101 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     5161 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     5221 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     5281 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     5341 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     5401 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     5461 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     5521 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     5581 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     5641 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     5701 cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg
     5761 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     5821 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     5881 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     5941 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     6001 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc
     6061 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     6121 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     6181 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     6241 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     6301 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     6361 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     6421 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     6481 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     6541 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     6601 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     6661 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     6721 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
     6781 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
     6841 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
     6901 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
     6961 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
     7021 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
     7081 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
     7141 cgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
     7201 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
     7261 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
     7321 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
     7381 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
     7441 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
     7501 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
     7561 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
     7621 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
     7681 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
     7741 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
     7801 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
     7861 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
     7921 cgatatcaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
     7981 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
     8041 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
     8101 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
     8161 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
     8221 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
     8281 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg
     8341 tgctataagt ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga
     8401 cactccaaca ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat
     8461 catgggcaga aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc
     8521 agagaaaaag aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt
     8581 cgtaaacgaa gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat
     8641 ctgggttgtg ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga
     8701 atggtatatc cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg
     8761 aacatatgaa gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt
     8821 gcattatgta cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc
     8881 ctgtacatag ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa
     8941 aatctagata cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac
     9001 cattttcatc aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg
     9061 gcacattcaa aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc
     9121 cacctggtga tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag
     9181 gtcttgagcc tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag
     9241 gagtaacgaa aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag
     9301 caggagcctg ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta
     9361 caaatacact ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt
     9421 tgtaagaatc ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt
     9481 tcctccttca aactatctat aataatctta caaaagataa gtaactggag caaataaaaa
     9541 cacttactga caaaagctac atagaagata gattacttat ttttaacaaa aaagaagtct
     9601 aaaatcttct attttctaaa aaaaatcgaa tttaatcaag ctgtaaagaa aaaaagcttt
     9661 acagggtaaa aaaaaactgt tacactacat cttgtgaata aaacaatgga ggtggaaata
     9721 aataatggca gttaaaatcc gtttaaaacg tatgggttct aaaaagagtc cgttttaccg
     9781 tatcgtcgta gctgattcac gttctcctcg tgatggacgt ttcatcgaaa ctgtaggtac
     9841 ttacaatcct ttgaaagacc ctgcagaagt agttttaaaa gaagacttag ttcttgactg
     9901 gttatcaaaa ggtgcacaac cttctgacac tgtacgtaac atcctttcaa aagaaggcgt
     9961 tatgcaaaaa caccacgaag ctaaattctc aaagaaataa ggtgaccgga tatgaaagat
    10021 ttaagcgact tagtcttaac aatcgttcgt ccgctagtca cttatcctga tcaagtcaag
    10081 ttagacgtcg tagagtcgaa agacttttac gagtacaatc taacagtggc tcctgaagat
    10141 attggccgta tcattggaaa acaagggcgt gttgctaaag caatccgcac aatcgtttac
    10201 ggtgttcgga tcaatgcacc gaaaaaagtg cgtttgaata tcatcgatga taaggaaaag
    10261 taaataaacg tgctgtactc gaattttcga gtgcagcttt tttgtttttt ttattaattg
    10321 aagaaatgat tatttggcaa aacaagcaag aaagcgtatc cttataggag gagggagtga
    10381 acagccaatg agaaagaaaa tgttatttat tccatttgct ctattgttaa gcgcttgtag
    10441 ttcggttact caagaatcag acaatacaca tctatcaagc agtatatctg aaactgttac
    10501 cagtccagaa aaaagtagta ccaaaacggc ttccaccact gaacaaacaa tggctacctc
    10561 aaataatgag aaaaagactg ctttagacca gttgaaagaa caacagccaa atgttcccat
    10621 gcctctagat gtaccagtct cttcaggcta tctcaatatc gcagcgacac atacaaagca
    10681 aggttattct attttatatt acagaacaga tcgtcccctc gggttgaatg ctgacgaatt
    10741 gaatcaagaa acgcctattg caacctatct atatcaatat gggtttgctt ctagtcaaga
    10801 aacaatccaa gttttgcagc catttgagat cgacacaaat ggtcagcaag tggacttagg
    10861 ttctcgaatc acagggtatc agcaaggtgc agcaggttca agttttcttg aatggcaaga
    10921 aggaaactgg cgtattcgaa tccgtgggaa taatatagaa ggccaagatc ccttactatt
    10981 agctaaagaa atcgtagctt atttggaaga aaacagtcta ccagcacctg aacaatttgg
    11041 taaaattact gttgatatgg gtgatacgac gaatcgagcc gtagaagtaa gctggcaaga
    11101 accgaaaaat gcctatacga tcactcatca agatcctatg tctgcgttga agatggcagt
    11161 ttctatgaag aggttgtgaa atcagcgcgc tgatctgagc ccatcattta actccaagtc
    11221 acaggaagta atcaaaaata gcacgccgca tttttgcata attttggctt aataccttaa
    11281 aagaaaacta gaattgcctc ttcacacatg ctaaaataaa acagacaaaa aagaaatggg
    11341 gatataagtt gactgaatac ttgaatgtag gaaaaatcgt aaacacacaa ggtatcaaag
    11401 gcgaagtccg tgttatctca acaacggatt tcccagaaga acgttacaaa aaaggaacgg
    11461 tgctaacact attccaagac ggaaaagcac ctgtggaatt aacagtcaaa agtcatcgca
    11521 aacataaaaa ttttgactta ttgagttttg aaggacatcc ttctatcaat gatgtggaaa
    11581 aatatcggga tggcatatta aaagtgtcaa aagacaactc tgtagatcta gctgaaaatg
    11641 aattctatta ccatgagatc atcggtctgc atgtattaga agatggaact gagttaggta
    11701 aagtaaaaga aatcttgtca ccaggtgcca atgatgtttg ggtcgttcaa cgtccgaaaa
    11761 aagctgatat attgcttcca tatatcgctt ctgtcgtcaa agaagtcgat ttagaagctg
    11821 gagtggtccg tgtagaaatt ccagaaggac tgattgacga tgaagattga tgtattgact
    11881 ttatttccaa gaatgtttga aggtcctatg ggggaatcca tcatcggtaa ggctgtagat
    11941 aaaggattat tagatatcaa tatctctaac ttccgcgatt attcagataa caaacatcag
    12001 acagtagatg attatccata cggcggtgga gcaggtatgc tgctcaaagt ccagcccatc
    12061 tatgataaca tccaagccat tgaaaaagct gcaccggatg tcaaaaaaag agtaattctt
    12121 cttgacccag ctggtaaacg attcgatcag aaaatggcag aagaattttc acaagaagaa
    12181 cacttagtat tcatttgtgg tcattatgag ggatatgatg aacgtatccg cacattagtg
    12241 acagatgaag tttccttagg agattatgtc cttactgggg gagaattagg tgcgatggtc
    12301 atgatcgatg caacagttcg gcttttgcca gatgtgttag ggaatcagac ttcagctcaa
    12361 acggattctt attcaaccgg tttattggaa catccccaat atactcgccc ggcagaatat
    12421 aaagggatga aagtaccaga agtattgaca aatggaaatc ataaattgat tgaagaatgg
    12481 caattaaaag aatcactaag aaggacctat caacgtcggc ctgatctttt ggaaacactt
    12541 gaatggacac ctcaaatgac gaaattttta gaagaaatca agaaagaaga acaagaaaaa
    12601 acagcagatg aagccaagtg actttttcta aaatcttctt tacagtaggt tgataatatg
    12661 ttagtatttt atggtgagtg caaaagcact taactattac gatattccgc tgtgagaaaa
    12721 gctcataaga atatttggaa taaggagaag aaaaccatga atccattaat cgaagaatta
    12781 acaaaagaac aattacgttc tgacatccca gcgttccgcc ctggtgacac tgtgcgtgtt
    12841 cacgcaaaag ttgttgaagg tactcgtgaa cgtatccagt tatttgaagg tgttgttatc
    12901 aaacgccgcg gtgctggaat cagcgaaact tatacagtac gtaaagtttc taacggagtg
    12961 ggcgttgaac gtacattccc attgcataca ccacgtgttg caaaaatcga agtagttcgc
    13021 tacggtaaag tacgtcgtgc aaaattgtac tacctacgtg cattacacgg aaaagctgct
    13081 cgtatcaaag aaatcagaag ataaacacgg aaaacttcct tggaaatctg aaatttgtga
    13141 aaagtccttg tatatcaagg gctttttctt ttttctctat cacagtttta agccattttt
    13201 ggctgttttg gaccattttt caatcatatt cccttaaaaa ttgtcctaga ttgtccgaca
    13261 ttgactatca gtgtcgtcaa aatagggcaa aatttagtag tcagcttgac gacaaatata
    13321 atacgaaaaa taccaaaaca tttaccgaat ttagcaactt ttgaacccaa gtttctcaaa
    13381 gggtcagcta ttagcaaaaa agacaaaaca tgtacttttc tactaaaaag ttgatgtgat
    13441 aggcttctat ctaaataact attaggagtt tttatcgtga agcaaaatga tggacgtatc
    13501 atttggaaaa atcaaagtga gttaaaactg attttaacaa ttaatgaatt catcgaaaaa
    13561 cacggaatta catctagccg tcaataccag aaaaagctat cagaaaatcc aaattctgca
    13621 ccaagtatgt ggttcattaa caaaaaatat ggatcatggg agaatttatt gattagtata
    13681 ggacgagaaa atactgggta tggaaaatgg gctagaatgt cagaacaaga attattggaa
    13741 atagtagaag cctttattaa gtgcgagaag ataacctctc aacgaatgta cgaacaaaaa
    13801 tcagtaggga agaatattcc ttcacttagt accattaaaa agatgttagg agatatacgt
    13861 ccacttttta aagaaaaaaa tgatggatct cgatttactg atttcgaact gctgttagaa
    13921 ctaaaaaatg aaataatccg attaaaacta caggatgatt tatcaatgac aaagtttcga
    13981 aaactagttc aatccccaaa actaccatca gttgatacaa ttatgaaaag aacgaacaaa
    14041 aattgggagg aattgatggc agagattgga tttgattatc gaagaatcaa aatttataag
    14101 cagagaaaca acctatccaa gacaaagaaa actaaatagt atcactctaa atcagacgtt
    14161 caaaattaaa atttggacgt cttttttatt acccaaaaac gaaaggagat ttattttatg
    14221 aaaaagccaa gatttaagaa ttggcgtttg ctcgcaacac tgagtctatt atgccaaaca
    14281 attggtggat cgcttggccc aacgattgcc tttgctgatg aaattactca tccacaaacg
    14341 gtaactgtgg aattggattt ggctcaccag tatgctgtcg aaggtacatt tagcgatggt
    14401 cgtcccatgt cagaagttac cgttccgcac tatgcagttt acaatggtgt gaagcaagat
    14461 attttctgta ttgagccagg ggtgccgatt tacaatgagt tcactcctgg ttatgagaaa
    14521 aatccattgc ctgatatgtc ggagaaagcg aaattagttt ccgttttatg gaaggaagct
    14581 ggtacggatg tggatacaca tattgtggcc cagaagatga tttggcaaga agttaatggc
    14641 tacacgcttc actcaattaa acgttctgat ggtagtgcag taaatatcgc agccattgag
    14701 tcaaaaatca atcaagccat tgcggattat caaaagaaac caagcttcca taactccaca
    14761 gtgaaaaccg tgttaggtca atcgactact gtgacagata cgaacggctt gaatttatcg
    14821 gagtttgatg aagttgtcga gaatatcgca aatattgatt atcgtgtgaa cggcaatcaa
    14881 ctagtgatta ctccaaatgc caattcaaaa gagagtggtg aattaaccct aaagaagtca
    14941 gctaatacag gaacaccggt agcttataaa atggctggtc aacaaacttt gatggcgggg
    15001 gcaattgata agcccaatac ctacacaatc aaaattgatg tggaaactga gggttctttg
    15061 aagattaaaa aagtcgataa agaaacagga aatgttgtac cgggaaccgt tttccattta
    15121 gactttggaa aaacattacc tgcaaaagac gtgaccaccg ataaagaagg cattgcgaca
    15181 ttggatgaga ttcctcatgg cgcgaaagta accattactg aaaaatcagt gccagctcct
    15241 tatacgattg acactacacc aatcaccgca accattaaat ctggtgagac tatttctgtg
    15301 acttcaaaaa atactcgcga aaaaggtcaa atcattttga ataaaatggg ggtggaaaca
    15361 ggaactgatc tttggaacga caaatactca ttagccggaa atacgtttgc cattcgcaaa
    15421 gacagtccaa ccggtgaaat tgtccaagaa atgaccacgg atgaaaaagg tcatgcggaa
    15481 acaccaaaag agattgccaa tgcgttggaa ttgggaacct attatgtgac cgaaaccaaa
    15541 gctagtaatg gctttgtgaa tacctttaaa ccagtaaaag tcgaattgaa ctatgtcaat
    15601 caaaccgtgg ctcttgttac cagtaacgta aaagggcaaa accaagaaat cactggtgaa
    15661 accactttga caaaagaaga caaagatacc ggtaatgaaa ctcaaggtaa agctgagttt
    15721 aaaggagctg aatatactct ctttactgca aaagatggtc aagcggtcaa atggagtgag
    15781 gcttttaaac cagagttagt gaagggaacg aaagcttctg atgaaacggt gactttgact
    15841 ttagatgaaa agaatcaagt tgctgttaaa cacttagcga ttaatgagta ttactggcaa
    15901 gaaaccaaag cacctgaagg atataccttg gatgaaacaa agtaccctgt ttccatcaaa
    15961 aaggtggatg ataacgaaaa aaatgccgtg attacccgag atgttacggc aaaagaacaa
    16021 gtcattcgct ttggctttga tttctttaaa tttgctggat cagctgctgg gactgccgaa
    16081 actggcttca atgatctgac ctttaaagta tcgccattgg aaggaaccag tgagattaca
    16141 ggtgccgaag atgaagcaat tacagcttat aacgagcaac taggttttga tggctatggc
    16201 aagtttgaaa atcttcctta tggggattat ttacttgaag aagtagaggc tccagaagga
    16261 tttcaaaaaa ttacgccatt agaaatccgt tctactttta aagaaaataa agcggattac
    16321 actaagagtg aatacatttt tacgattacc gaaaaaggac aaaaacaacc aattaaaacg
    16381 gtgaccgttc cttacgagaa actgacgaat aaagcatttt ctgttagctt gaatcgtttg
    16441 atgctctatg atttacctga agaagaagat agcttaactt ctcttgggac ttggaaagac
    16501 ggaggcaaag aattgacaga ccttgattct accgagctag ttgataaatt gagctataac
    16561 ttgcatgaaa tcaaagaaga ctggtatgtg gtagcgcaag ccattgatgt ggaagcgaca
    16621 aaagctgccc aagaaaaaga tgaaaaagcc aaaccagtgg taattgccga aacaaccgca
    16681 acgttggcga ataaagagaa aacaggaact tggaaaattt tgcataaatt aaccgcagaa
    16741 caagttttgg ataaaaccat cgtcttattc aactatgtgt atgaaaacaa gaaaccctat
    16801 gaagcaggtg atgatccagt agcgaaagat gctagcttga acaatcaagc ccaaactgtc
    16861 aattgtacgg tggaacgcca tgtttccatc caaacaaaag cccacctaga agatggttcg
    16921 caaaccttta ctcatgggga tgtggtggat atgttcgatg atgtgtcgat tacccatgat
    16981 gtactggatg gctcaaaaga agcttttgaa acaattctgt atgcattact tccggataat
    17041 acgaccaaag aaatttggaa atctggcaaa attgattatg aagtgaatga caaagaattc
    17101 actaaaaccg tacttgcgga aaaagtggac acaagtaaat atccagaagg tacaagtttt
    17161 accttcaaag aaattaacta tggcaaagac ggaaacatca atgggaaaca caatgaggat
    17221 ctaaaagaaa aaactcaaac tttaacacct aaaaaagttc cgacaacacc aagtgcacca
    17281 gaacaaccgg aaataccaac tgttccaagt gactctcaag aatctagtcc tacagtaaag
    17341 acattcccac aaacagggga gaaaaattct aatgttctgc tgtttattgg gtttaccttg
    17401 atctttgcga ctgctgggta ttatttctgg aatcgccgaa actaaggtga tgtgatgaaa
    17461 caactaaata ggaaaaacca acgtgcgcga ccgcctccta aaaaaagtgg tcgcgctatt
    17521 tttgtatcca aaactaaaaa tgaaaagagg aaaagaagat ggaattgaaa tttgttgtgc
    17581 cagatatggc cgaaaccttt ggaaagataa ggtatgccgg cgaaggcgaa gttttaactg
    17641 aaggatatgg gcggaatact acagtaattg gtcgtagtta tcatttgtat tccagtaagc
    17701 aacgagccga tgatattgaa gtggtggtag ctgcggaagc cggcgaaaag gattttgatc
    17761 aagatcaacc actaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga
    17821 atcgtgggtt taccgactac gtgttgtatg tcgatgattt agtgaaagca taggaggaaa
    17881 agaagatgag attaacagaa ggtattgttg tagattcagg actaacgttt gggaaattac
    17941 gattttccgc attacgccga gaagtacgga agcaaaatga ggatggaacg gttagtaacg
    18001 aggtaaaaga acggacttat aatttgaaat cctctgcgca aggtcgaatg atccaagtta
    18061 gtatcccagc aaatgtaccc ttacgcgagt ttgcctatga cgcagaggta gagttggtca
    18121 atccaatcat ggatacggtc gccaattatg ttttccgaga aggaactact gtcaattggt
    18181 tcattaaggc cgatgattta gtcttgaaac gacaaccaga tcagggaaat tctacaaacc
    18241 aaaatgaagg aaagaaatag gtgaagcgta tggaaattaa tgttgaacat attttagatt
    18301 gtctcgatca atatggaaaa ggtgagctaa cggaggaaca actcactaaa gcattaacct
    18361 atgatgaaaa aatgtttctg attatgcatc aagggttact ggaagtaggt aacgatgtcg
    18421 aagaagattt tgatgtactg aattggttag gggaagaaga gtccttcttt atggttatcg
    18481 aagtaaatga aagtctatgt cgagaagccg aaaccgtatt ggaagaaatt ggtgtggaaa
    18541 tgccggatgc cattgaagtt tttttaaagc aactagtgga aacgaaacaa ctccctatag
    18601 cagtaaatga ctagcataac tgcttgaaat tggatcaatg aaatcctatg aagctttttt
    18661 cgattggagg tggaaaccat aaaaatgtac aaagggcatc gtatacgggc aggagatcag
    18721 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    18781 gtcttttatt ttcaagagct tagacaattt gatatttcta agctatcact ttctactata
    18841 gaaatcgttc gttctataaa agatttaatc tatttgctag gaagtctcgt tctttcagga
    18901 agcacgatgt tgctttatat acactttttt caggatcatt ggcgcagttt gtggcatcga
    18961 caaaagctag cccggatgat tctagaaaat cattggtacg aagtaaaaca aactcagagt
    19021 gaaggatttt tcaaagacct caatagtagt cgaaccaaag agacaatcag ttacttcccc
    19081 aaaatctatt accgtatgaa ggatggttta ctttctattc gcgttcaaat ttcactggga
    19141 aaatatcaag atcagctttt aaagctggaa aagaagttag aaagtggtct atattgtgaa
    19201 ctagtagaaa aggaactcaa agactcctat gtggagtaca ctttactgta tgatataatt
    19261 gccaatcgaa ttgggataga cgaagtagtg gcagaaagtg gggctttgcg attgatgaaa
    19321 aatcaagtat gggcttatga ttctttacct catatgttaa tcgctggcgg cacaggtggt
    19381 gggaagactt actttttact cactatcatc gaggctttat taaaatctga tgccgagctt
    19441 tttgttcttg atcccaaaaa tgcggactta gccgatttag gtacggtaat gcctcatgtc
    19501 tattcccaaa aggaggaaat ttctgcttgt gtggaagatt tctatgagcg catgatgact
    19561 cgtagcaagg caatgaaaga aatgcccaac tacaaaacag gagagaatta tgcgtttctt
    19621 ggacttccac caaacttttt aatctttgat gaatacgtgg tttatatgga aatgttaacg
    19681 acgaaggaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt aggtcgtcaa
    19741 tctggtttct ttctgattct agcttgtcaa agaccggatg cgaaatattt aggagacggg
    19801 attcgcgatc aatttaattt ccgggtggct ttgggtcgta tgagtgaact gggatattcc
    19861 atgatgtttg gcgaggttga taaaaaattc tttatgaagc gtatcaaagg tcgaggctat
    19921 gtagatacgg gagggagtgt gattagtgag ttttatacgc cacttgtacc aaaaggatat
    19981 gatttcttaa gggaaattag cgacattggt ggattaaacg ttaatacgga aggataaaat
    20041 aatagtatgt agcctaggag tgtcaagaat cctgggcttt ttttacgagg tgatttatga
    20101 catttttaga tttattcgct gggattggtg gcttttgctt aggtatggaa caagcgggtc
    20161 accagtgtat cggcttttgt gaaattgatg attttgctcg ccagagttat aaggcaatcc
    20221 atgatacaag taaggaggtg gaaatgcatg acatcacaag tgtatcagac gagtttattc
    20281 aatctctcgg accagtggat atcctttgcg gtggatttcc gtgccaagct ttttcaattg
    20341 cgggaaagcg gcaaggattt tccgatactc gaggtacttt attctttgaa atcgctcggt
    20401 tcgccgctat tctccaacct aagtttttat tccttgagaa cgtccgggga ttactcaatc
    20461 acgaaggagg ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgatg
    20521 tggaatggca agtgcttaac tcaaaggcct acgttcccca aaaccgtgag cggatcttcc
    20581 ttatcggaca ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggatcgtctc
    20641 caacatctga tcaaaacatc agaaactta
//
LOCUS       DACJGB010000055        20665 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.055, whole genome shotgun sequence.
ACCESSION   DACJGB010000055 DACJGB010000000
VERSION     DACJGB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.055"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            169..363
                     /locus_tag="IYI34_002049"
     CDS             169..363
                     /locus_tag="IYI34_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559912.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            632..931
                     /locus_tag="IYI34_002050"
     CDS             632..931
                     /locus_tag="IYI34_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559913.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1028..1462)
                     /locus_tag="IYI34_002051"
     CDS             complement(1028..1462)
                     /locus_tag="IYI34_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0559914.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1694..2404)
                     /locus_tag="IYI34_002052"
     CDS             complement(1694..2404)
                     /locus_tag="IYI34_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAR0559915.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2394..3254)
                     /locus_tag="IYI34_002053"
     CDS             complement(2394..3254)
                     /locus_tag="IYI34_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAR0559916.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3259..3897)
                     /locus_tag="IYI34_002054"
     CDS             complement(3259..3897)
                     /locus_tag="IYI34_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAR0559917.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3912..4211)
                     /locus_tag="IYI34_002055"
     CDS             complement(3912..4211)
                     /locus_tag="IYI34_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0559918.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4254..5738)
                     /locus_tag="IYI34_002056"
     CDS             complement(4254..5738)
                     /locus_tag="IYI34_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAR0559919.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5758..6219)
                     /locus_tag="IYI34_002057"
     CDS             complement(5758..6219)
                     /locus_tag="IYI34_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR0559920.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6237..7304)
                     /gene="ulaG"
                     /locus_tag="IYI34_002058"
     CDS             complement(6237..7304)
                     /gene="ulaG"
                     /locus_tag="IYI34_002058"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAR0559921.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7522..8292
                     /locus_tag="IYI34_002059"
     CDS             7522..8292
                     /locus_tag="IYI34_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR0559922.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8565..9392)
                     /locus_tag="IYI34_002060"
     CDS             complement(8565..9392)
                     /locus_tag="IYI34_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0559923.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9960..11195
                     /locus_tag="IYI34_002061"
     CDS             9960..11195
                     /locus_tag="IYI34_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAR0559924.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12194..12454)
                     /locus_tag="IYI34_002062"
     CDS             complement(12194..12454)
                     /locus_tag="IYI34_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAR0559925.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12481..13782)
                     /locus_tag="IYI34_002063"
     CDS             complement(12481..13782)
                     /locus_tag="IYI34_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0559926.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14074..14532)
                     /locus_tag="IYI34_002064"
     CDS             complement(14074..14532)
                     /locus_tag="IYI34_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAR0559927.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14545..15870)
                     /locus_tag="IYI34_002065"
     CDS             complement(14545..15870)
                     /locus_tag="IYI34_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAR0559928.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15887..17299)
                     /locus_tag="IYI34_002066"
     CDS             complement(15887..17299)
                     /locus_tag="IYI34_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0559929.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17316..18080)
                     /locus_tag="IYI34_002067"
     CDS             complement(17316..18080)
                     /locus_tag="IYI34_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAR0559930.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18184..19059)
                     /locus_tag="IYI34_002068"
     CDS             complement(18184..19059)
                     /locus_tag="IYI34_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0559931.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19314..19398)
                     /locus_tag="IYI34_002069"
     tRNA            complement(19314..19398)
                     /locus_tag="IYI34_002069"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19362..19364),aa:Leu,seq:caa)
     gene            complement(19416..19487)
                     /locus_tag="IYI34_002070"
     tRNA            complement(19416..19487)
                     /locus_tag="IYI34_002070"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19453..19455),aa:Gln,seq:ttg)
     gene            complement(19497..19569)
                     /locus_tag="IYI34_002071"
     tRNA            complement(19497..19569)
                     /locus_tag="IYI34_002071"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19534..19536),aa:His,seq:gtg)
     gene            complement(19631..19715)
                     /locus_tag="IYI34_002072"
     tRNA            complement(19631..19715)
                     /locus_tag="IYI34_002072"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19679..19681),aa:Leu,seq:caa)
     gene            complement(19736..19806)
                     /locus_tag="IYI34_002073"
     tRNA            complement(19736..19806)
                     /locus_tag="IYI34_002073"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19772..19774),aa:Cys,seq:gca)
     gene            complement(19852..19923)
                     /locus_tag="IYI34_002074"
     tRNA            complement(19852..19923)
                     /locus_tag="IYI34_002074"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19889..19891),aa:Gln,seq:ttg)
     gene            complement(19935..20007)
                     /locus_tag="IYI34_002075"
     tRNA            complement(19935..20007)
                     /locus_tag="IYI34_002075"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19972..19974),aa:His,seq:gtg)
     gene            complement(20010..20083)
                     /locus_tag="IYI34_002076"
     tRNA            complement(20010..20083)
                     /locus_tag="IYI34_002076"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20049..20051),aa:Trp,seq:cca)
     gene            complement(20090..20170)
                     /locus_tag="IYI34_002077"
     tRNA            complement(20090..20170)
                     /locus_tag="IYI34_002077"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20134..20136),aa:Tyr,seq:gta)
     gene            complement(20172..20247)
                     /locus_tag="IYI34_002078"
     tRNA            complement(20172..20247)
                     /locus_tag="IYI34_002078"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20212..20214),aa:Phe,seq:gaa)
     gene            complement(20283..20355)
                     /locus_tag="IYI34_002079"
     tRNA            complement(20283..20355)
                     /locus_tag="IYI34_002079"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20320..20322),aa:Asp,seq:gtc)
     gene            complement(20365..20437)
                     /locus_tag="IYI34_002080"
     tRNA            complement(20365..20437)
                     /locus_tag="IYI34_002080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20402..20404),aa:Val,seq:tac)
     gene            complement(20453..20524)
                     /locus_tag="IYI34_002081"
     tRNA            complement(20453..20524)
                     /locus_tag="IYI34_002081"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20489..20491),aa:Glu,seq:ttc)
     gene            complement(20539..20628)
                     /locus_tag="IYI34_002082"
     tRNA            complement(20539..20628)
                     /locus_tag="IYI34_002082"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20592..20594),aa:Ser,seq:gga)
BASE COUNT         6531 a         4319 c         3275 g         6540 t
ORIGIN      
        1 ttaagaacaa tagcctttta ttatatatat tattcaaatt agttatatcg agtgaaaaaa
       61 actttcaaat tgattctttt tatttatcat aaagatatag atacataatc attttcactt
      121 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
      181 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      241 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      301 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      361 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      421 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      481 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      541 gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt
      601 ataatgtatc agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga
      661 actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa
      721 agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc
      781 aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc
      841 tttaaaaaaa aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt
      901 tgataaagag gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata
      961 ctaagtctat cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta
     1021 ttatatgtta tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag
     1081 aaataccacc catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt
     1141 tctcagtatc atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca
     1201 aagcttcatt caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga
     1261 tatcttgaat aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct
     1321 tcccttcccc attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg
     1381 cagtgtcttt tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga
     1441 tcataaggga atcaatattc atttttttct tcctttctta tacaattctc cctctaataa
     1501 tacagttctt tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt
     1561 ccttaaaatt cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat
     1621 aaattttctt cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct
     1681 taccctagca tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt
     1741 tatctagtaa atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg
     1801 ccatcagaca aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc
     1861 cccatgtgaa cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt
     1921 ttctttcctt aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta
     1981 cttcttcttt cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag
     2041 tcgtgccata agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat
     2101 gagtatgagt aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga
     2161 gatctgaaga tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca
     2221 ccatttgatc tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc
     2281 ctagctcacg gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg
     2341 gcaatgccag atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag
     2401 acatcatagc cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct
     2461 cttcgaaagt cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct
     2521 agacgtttca aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca
     2581 tcacgaaact ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca
     2641 gtgatataat cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac
     2701 aaattgccta ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct
     2761 aaaaattttg aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg
     2821 ccatatttag aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa
     2881 ttcattgatt tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt
     2941 agattatgcg ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct
     3001 ggatcagcag aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt
     3061 acaccggagc tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat
     3121 cgtgctaacc gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct
     3181 tttacaaggg aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc
     3241 ccaatttgag ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc
     3301 ggccttcatc ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc
     3361 gatcccttca aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa
     3421 gcctagttca atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga
     3481 taacagcgca tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc
     3541 catggcttgt tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt
     3601 ttgtgctgcc tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc
     3661 tgcttttgca acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac
     3721 tagtttttta tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa
     3781 gcaaagaatt gtgcccactt caataatatc tactacatca ccaactttca taacatctcc
     3841 taatgcatgt tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt
     3901 agttccttcc tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt
     3961 tctttgactg acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga
     4021 gtaaacatat tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt
     4081 tttccctctg aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt
     4141 gctttttcca cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact
     4201 aaaattctca tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact
     4261 ctctgctttt ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt
     4321 ttccgatatt gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca
     4381 taacctagga ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc
     4441 ccgatataac caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct
     4501 acttgtatga ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta
     4561 ttggcataaa ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg
     4621 atcgttggtg atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca
     4681 aacaagaacc caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca
     4741 attcctggaa cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt
     4801 tcatcgacga atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa
     4861 gaagtcgtca taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc
     4921 aaataatctt tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa
     4981 gtagcaacca tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt
     5041 tttttgtctt gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct
     5101 aattttgcaa atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct
     5161 tctgtcaatt cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct
     5221 aaaatcagtc ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt
     5281 aaaatccaaa aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa
     5341 cgcagtttcg tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt
     5401 aataaaatca tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca
     5461 gcattttgtc caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt
     5521 ccaaccaaga ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag
     5581 taaccgaccg ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa
     5641 atatagccta ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt
     5701 gcaaaatacg tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta
     5761 attatattct gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc
     5821 aaccaatcca tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc
     5881 tggatctttt gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc
     5941 actttcaaaa cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc
     6001 atcacttttt tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc
     6061 gtatttttct acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc
     6121 gatcaataca agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg
     6181 gtaatgaatc aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat
     6241 aaaaacgacg gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa
     6301 aaattaaact caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag
     6361 atataaggtt taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc
     6421 tcttttggat cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt
     6481 gcattcaatg actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct
     6541 cgtgggtttt caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg
     6601 gcaaataaat tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg
     6661 aataaatagt ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct
     6721 tctggatctt cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg
     6781 atgtctttga ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt
     6841 ccccattttt gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa
     6901 ttttgatgaa ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg
     6961 attaaagcgt ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa
     7021 ttaggctgca tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg
     7081 ccattcccat gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct
     7141 tcatgacttt tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga
     7201 tccactactt ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct
     7261 actaaccact tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca
     7321 tatcgttgtt ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt
     7381 tatatgaccg tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata
     7441 ataaagacag atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat
     7501 gtccctcagg aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa
     7561 gaaattttgg aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc
     7621 cttgatgtat ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag
     7681 atcatacgca attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa
     7741 tctggtcctg aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat
     7801 tatgatacgc tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa
     7861 tctaaccatt tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat
     7921 aattgtagtt atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc
     7981 gacatcgcat taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc
     8041 ggagtcgatg tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa
     8101 atgatggtca acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga
     8161 ctgacatcaa agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa
     8221 ttttgttctg aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta
     8281 gattcatttt aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac
     8341 aaatttatcg ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt
     8401 atccatccaa tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct
     8461 aaataggaat caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt
     8521 cgaataaacg gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt
     8581 tcaatcacct ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata
     8641 tatctttcta aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc
     8701 aagtattcca atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat
     8761 aattggattg ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat
     8821 acaatgcaga ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc
     8881 acataaactt tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca
     8941 gctgcttgta agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa
     9001 aataatgctg catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa
     9061 atttgattta aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta
     9121 taagattctt ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg
     9181 attttgtctt cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa
     9241 agcgcactta agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt
     9301 gcttgttcac aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat
     9361 tcttttagta atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat
     9421 ttctccttta tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa
     9481 taaaaatttt aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat
     9541 ttctttttgt gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc
     9601 atggcgagaa agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa
     9661 tgacgacagc acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta
     9721 tttttaatga cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt
     9781 gttgcgtgtt tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt
     9841 tgtgaaaaca gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt
     9901 gttttttagt atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca
     9961 tgaagacaat tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg
    10021 aggttgccaa agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt
    10081 ttgctccttt gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga
    10141 aaaaacaaga agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta
    10201 ccaaagaaat ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc
    10261 ctcgactgat tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga
    10321 ttcctttagt atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg
    10381 cgctgatgcc ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga
    10441 caaaaaagcg tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa
    10501 aagaaatgct tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca
    10561 tcacaacgcc taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg
    10621 gtccaatttt tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc
    10681 gaaagcaaca agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc
    10741 gtatgttacg caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac
    10801 ctattgacta tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat
    10861 atctgtctag tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag
    10921 ggaccgtcca aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg
    10981 agcaaagctg taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac
    11041 cagtgactga aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc
    11101 agcaggcacg cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag
    11161 aaatcgaaac cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa
    11221 tagagactca tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta
    11281 aatagtggat tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat
    11341 ccttaaaata aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc
    11401 ttagtcaaat tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa
    11461 agaccataat aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat
    11521 cgctttgtag agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt
    11581 taccacttta tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc
    11641 gatgcatact cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa
    11701 tgttctatgt cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt
    11761 cccagcacaa ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata
    11821 ttaccattca tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata
    11881 tttgacttga tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt
    11941 ccattttttc ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa
    12001 aagggctgtg acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc
    12061 ttgcatctgc ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt
    12121 aagaagcaat tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc
    12181 acttttagaa catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat
    12241 tgatagcaaa aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg
    12301 gtacaatgat ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat
    12361 tgatttgaat atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa
    12421 agataaaaac aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac
    12481 ttagacagtc tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc
    12541 ataagctttt acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa
    12601 gataacaaca cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt
    12661 ccatgttggc aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat
    12721 aatagtcaaa actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat
    12781 aggagctcca aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg
    12841 accaacctct ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat
    12901 cgttgctcct gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata
    12961 gggtaattgg gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat
    13021 cagccacatt ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca
    13081 caacaaatgg atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt
    13141 ggttaaaggc tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg
    13201 aatcagccaa gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc
    13261 aacagcaggt ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg
    13321 atatacttcc actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa
    13381 agaactcata ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg
    13441 aacttgtaac ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc
    13501 atatgattta gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat
    13561 cgtcatccta aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc
    13621 aggaaacggt aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt
    13681 tgaaacaacc ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg
    13741 tgggacaagt ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca
    13801 ataaaatatg aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa
    13861 ttaagaaatt ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg
    13921 aaaataagtt ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa
    13981 atgattaaat ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata
    14041 aatatcaccc tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat
    14101 ctttacttct tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat
    14161 gatcaccgta tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt
    14221 cccttctttt acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc
    14281 aactgtatct aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat
    14341 ggcatgtcgg gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc
    14401 atcatgattc gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt
    14461 tactgctgtt aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt
    14521 aaagaaatgc actttttatc acctctactt taatgctggc caatattttt tgtttacttt
    14581 gatcatttca tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt
    14641 aaatgcttgt aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc
    14701 tactgcaact tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt
    14761 aattggctcc acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg
    14821 tagattcgga atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt
    14881 caacaaggaa atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa
    14941 tacttcttcc acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg
    15001 tccatccatg accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc
    15061 aggatataag taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat
    15121 gactgacatc atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac
    15181 ttgcatttct tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc
    15241 tttatcataa atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca
    15301 attcttacga tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc
    15361 acaggcatta atcattttta cattcggata tttacgacga acagcttcag aaacgatcga
    15421 ctctggattt gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac
    15481 aatttccaat aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt
    15541 ttcttgtcct actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa
    15601 tcctccaaca cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc
    15661 atccgttgca cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat
    15721 caagtacata tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg
    15781 caaccgtttt tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc
    15841 tccagctata gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata
    15901 atttattgaa ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt
    15961 tttcagttgg tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca
    16021 tggtgggatc cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat
    16081 tggcttttcc tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg
    16141 ctaaccctaa ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac
    16201 gtcctaatgt ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg
    16261 caaaaaagaa aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga
    16321 tgagataatc aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac
    16381 agacaatccc tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa
    16441 acatgaaact aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc
    16501 ttcccatgat tcctttgacc atctctttat tttctttata agctgcttta tagatagcta
    16561 gacatatagc tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag
    16621 cctgaggatc tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag
    16681 atgtatagct tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg
    16741 ggatcagcaa acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga
    16801 gcagttttgc aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa
    16861 cagctgcgat tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat
    16921 aagaaaaaac tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta
    16981 aaccggaaat aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc
    17041 cttttttcaa ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat
    17101 ttttgacaaa accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg
    17161 catttccagc tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga
    17221 tcaatcccgc taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca
    17281 catttgattt aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac
    17341 tcaatcaaag gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact
    17401 tggtgggtac gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct
    17461 gcgtgacaca tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa
    17521 tattcttcca cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg
    17581 aaacaaggct caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga
    17641 accggcaaat catatttttt tgctaatgta acaactacct cctgattttc atccaaagta
    17701 tgagtatgat gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga
    17761 atctgagcat tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa
    17821 taagataagg atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt
    17881 ttgccacacg tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt
    17941 ttcacagcat gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct
    18001 cgctgataag catccataat cccataattc acaccatggc tatagccaaa atcatccgaa
    18061 ttaaaaatca cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca
    18121 aataatcata gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt
    18181 attttaatag attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc
    18241 aagttgaaat tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct
    18301 atgtttaaat gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat
    18361 caagcgttta accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt
    18421 atcataaacg tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac
    18481 tatttttaag ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt
    18541 gatgaatcct acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac
    18601 cacttctact gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat
    18661 tgctgtgaca tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa
    18721 tgtaatatag atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac
    18781 ttcttccgca aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt
    18841 gatcttttct ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt
    18901 actcacttta gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc
    18961 atcgatcaat acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc
    19021 tctatatgat aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca
    19081 atttttaaag acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa
    19141 tagtagcacc ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata
    19201 tcttttttac tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa
    19261 aaacaaaaaa aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc
    19321 ggccggggtc gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc
    19381 caattccgcc acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac
    19441 gcatgaggga gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg
    19501 aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc
    19561 cacattcgcc atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca
    19621 tcttagtcaa taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt
    19681 gagtccagcg cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg
    19741 gtaaccggat ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat
    19801 accgccatta tactattatc aatctataaa ataaagaata aattatagaa actggggtag
    19861 ctggattcga accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc
    19921 ccaagaataa aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg
    19981 gtgcgttaac cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact
    20041 aacggttttg gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt
    20101 ggattcgaac cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg
    20161 ctactcctcc atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg
    20221 ttgctctacc aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac
    20281 gacggttccg acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc
    20341 ctacaccacg gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct
    20401 tgtaaggcag atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt
    20461 acgggattcg aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg
    20521 ggccaggtaa atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca
    20581 tctacatgat ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa
    20641 gctcaatact ctttctcaaa atgag
//
LOCUS       DACJGB010000056        20128 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.056, whole genome shotgun sequence.
ACCESSION   DACJGB010000056 DACJGB010000000
VERSION     DACJGB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.056"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            133..636
                     /locus_tag="IYI34_002083"
     CDS             133..636
                     /locus_tag="IYI34_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAR0559932.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            763..1800
                     /locus_tag="IYI34_002084"
     CDS             763..1800
                     /locus_tag="IYI34_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559933.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1815..3512
                     /locus_tag="IYI34_002085"
     CDS             1815..3512
                     /locus_tag="IYI34_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAR0559934.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3505..3966
                     /locus_tag="IYI34_002086"
     CDS             3505..3966
                     /locus_tag="IYI34_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559935.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            3979..4839
                     /locus_tag="IYI34_002087"
     CDS             3979..4839
                     /locus_tag="IYI34_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559936.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4877..5266
                     /locus_tag="IYI34_002088"
     CDS             4877..5266
                     /locus_tag="IYI34_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR0559937.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5253..7700
                     /locus_tag="IYI34_002089"
     CDS             5253..7700
                     /locus_tag="IYI34_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR0559938.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7705..9813
                     /locus_tag="IYI34_002090"
     CDS             7705..9813
                     /locus_tag="IYI34_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR0559939.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9810..10814
                     /locus_tag="IYI34_002091"
     CDS             9810..10814
                     /locus_tag="IYI34_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAR0559940.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10833..11744
                     /locus_tag="IYI34_002092"
     CDS             10833..11744
                     /locus_tag="IYI34_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR0559941.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            11931..12218
                     /locus_tag="IYI34_002093"
                     /pseudo
     CDS             11931..12218
                     /locus_tag="IYI34_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12406..12621)
                     /locus_tag="IYI34_002094"
     CDS             complement(12406..12621)
                     /locus_tag="IYI34_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559942.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12880..13047)
                     /locus_tag="IYI34_002095"
     CDS             complement(12880..13047)
                     /locus_tag="IYI34_002095"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0559943.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13392..14831)
                     /locus_tag="IYI34_002096"
                     /pseudo
     CDS             complement(13392..14831)
                     /locus_tag="IYI34_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15379..16050)
                     /locus_tag="IYI34_002097"
     CDS             complement(15379..16050)
                     /locus_tag="IYI34_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAR0559944.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16399..17850)
                     /locus_tag="IYI34_002098"
     CDS             complement(16399..17850)
                     /locus_tag="IYI34_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAR0559945.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(17962..19305)
                     /locus_tag="IYI34_002099"
     CDS             complement(17962..19305)
                     /locus_tag="IYI34_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR0559946.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(19439..>20128)
                     /locus_tag="IYI34_002100"
     CDS             complement(19439..>20128)
                     /locus_tag="IYI34_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR0559947.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT         6498 a         3414 c         3743 g         6473 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcctactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatgcgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cctgtcgatt ttgatgaagt gaaagaacag
      241 attggattaa atgacgaata tgaagagtac gcgattcatg attatgaact gccttttgag
      301 attgatgagt atacgccaat tgaagagatt aatcggttgt gtaacttagc ggaggaacta
      361 gaaggaacgc caattggtga agctgcttct gagattcaac acgctttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatt gtctattatc cagattgcga tgatatgagt
      481 gatgtggcgc attacttgat tgtagaagct ggtgattttg gcgaagttcc agaacaattg
      541 aagccgtata ttgattttga tgcttgcggt cgagatttgg agattggagg aaattatttg
      601 gttactagtc gtggagtttt tgagtatcca gaatgaatca aacactatat atagtgtttg
      661 attagtatat gtaagcaaaa aaacacaata tatagagtgc aaactgaagg gtaatgtgat
      721 ataatatttg ccgaagaaat atttttgaaa tgaggtaata ttatggcaaa tattcaaaca
      781 agttttattg atttccataa ttcaattaga ttggacgtag aagacaatac gctacttaag
      841 gactataaag accaagtaat tgatggatta aaagattact tacctgatga tgtgaagttt
      901 gaaacttttt tacagggaag ctattctgtt tataccggaa taaaaagctg tgatgaaaaa
      961 attgattttg acatcgatat tgcagttgct ttcgaaattg atcatactgt ttatgaagat
     1021 ccaagggagc ctaaactgtg ggtaaaagag gcgctagttg aaatttttcc taatgctcaa
     1081 gttaatttaa aagtaccttg tgtaactgct acatttactg gaaaaaagac aaaaaaaaat
     1141 gtgcacgttg atgttgcggt ttatgctaaa gaagatgaaa attatttctt agcaaaagca
     1201 aaagaattca gtgctcccga aaaccgatgc tgggaggaag cggatcccaa agtattaaaa
     1261 gagaaaatta atagtcatgt agcagattca gatgatcgaa aacaatttcg aagatgtatt
     1321 cgctatttaa aaagatggaa agataacaat tttaaccaag aatataaacc aacaggaatt
     1381 ggacttacaa ttaatgtgat ggatacgttc ctagttaata agtccactga ttttttaact
     1441 agaaaagttc agtataatga tatggaatgt atgaagcaga ttgtttcttc gctgaaagat
     1501 tcatttgtgt atgagtacag tgaaacagat ggttggcatt atcgtctaca tgcatatctt
     1561 ccagtgaagc ctaacagtga cacctattca aaaatgacag tgaatcagat gagtgatttt
     1621 aaaaacaaac tttctaaatt atatgatgat ttgattttcg ctatcgatac tgaagatgag
     1681 tatgaagcta caaaacgatt aaataatcaa tttggggagg attttttaat tatttcagaa
     1741 gaagaagtta cagaaaaaaa cttaagaaat gcatttgtta cagactatcc gagtgcttag
     1801 gttggtgtta aagattggat tatactgata ttttaaaaga aaagctaaca gatgttgtgc
     1861 gtttattcga tggtaattac tatggggatt tttcatttgc tacaattgga aagttgcgtt
     1921 taaaaattga aataccaaaa cattttccat tggtacttcc taggatttac attcataatt
     1981 ttgatcattt aggactttac ctaccacatg tagaaaaaga tgggttggta tgtttttctt
     2041 cagatataaa ttatttagta gatgaagaaa attcagaatt ggttttattg cagtgtgtta
     2101 ataaaactat aaaaacaatt gaggaaggtt tgaaagggtt aaataaaaat gattttcgaa
     2161 aagagtttgt tgctttttgg gacaaacaag agaatcaaat ttcgttattc tacctttgtg
     2221 aaccagcgtt gtttatcaag tcagttgata caataatgta cggtaagtct ttggttgctt
     2281 tggataaaga gaacgaatta caattaaatg cagcgagtag attgtttaat ggagagaaag
     2341 aagatactca tgaattagat gcatactatg ttccaattag aaacaagaat aatattatgc
     2401 ccccgaagaa taataaagca tggtcaaaga aggatatttt acaaatagta aaaaaaaatg
     2461 taacaggtgc aaataaaaga ttttttttaa aacttgaaaa aagaaagaat agacataatg
     2521 ttttacttat tctagatatc ccaattgaag aaaagtcaaa taatgtcata attggatttc
     2581 tttttacaaa acaatctgga tctataaaag attataaggg tacgcctagg actgtattag
     2641 ttcagcgtca tgatgaatta tatttggtga atagaacgag cggagaaaac tatggattgg
     2701 gtgctaatat agctattgtt ggggtagggt cagttggcag taaaattgct gaaaatcttg
     2761 gtccattagg tgtgagaaag atgacactca tagacaatga ttgttttcaa gctgaaaatt
     2821 tgtatcgaaa ttcacttggt gcagatagtt taaaaaatgc ccgatctcag aataattttt
     2881 ttaaagttga tttaatgcag gaggagctcg aaaaaaaaat cccatacctt tgtgttgaag
     2941 tggagtcaat gaacgtttta gacttaatta gtatgcaacc tggttttttc aaggaatatg
     3001 actatgtttt tgtttgcata ggagatacaa tggcaagcct ggctttaaat aattattttt
     3061 ccaaaagtag tcaaaaagtt ttctattctt ggctggagcc atatggcatt gggggacaca
     3121 ctctatttat aaattattct ctaaatcaaa aaggttgttt caattgtctt tatacagatt
     3181 caaactctaa aaataggtgt tcaaatagag cttctctggt aaaagaagga caaatttttg
     3241 aaaggacttt agcgagttgc cgctcaaaat ttataccata taatgctatt gcctcgacgg
     3301 aatgtgcaat taatgcaata aaacttttcc ataaagtcaa tcaaaataat atgaccaata
     3361 gtactctttt ttcatggata ggagatggga gtacttttga gaatttagga tatcaatata
     3421 gcgacagaaa tacttttctt aatggagtca ataagtttga agaaagattc ttttctaaat
     3481 attgcaaagt atgtggggag ttagatgatt aaattttcgt gtgaccgtat tgtcattgaa
     3541 gtaagggatg aagttcttaa gatatttgaa caatatcggc agggaaagga tgacttgcct
     3601 gaagctggag gagttcttca tggaaaaaga cttaagaatg ggagcataat aattaccgat
     3661 ataacaacac ctcaaatagg agatatacaa aaaagattac tctttaaaaa aaaccgagaa
     3721 attcatcaac agctttctaa tgagaaatgg atagaatcaa ataagttttc tattgcattg
     3781 ggagaatggc atactcatcc tgaagctgta ccaactccat caaatgttga taagaaaagc
     3841 tggagactga acgtgagcaa gcaacatgat gatagagttt atgtttttat tatcgttgga
     3901 ttttctgatt tgagaatgtg gttactgtca aaaaaatcag atattaggca ggctataaaa
     3961 ctataaagga gtgattaggt gaaatcacaa aagaatattt ggtggtgggg gtttttagta
     4021 ggaaaggtaa tgataccagc ccttctctta atttctctac cgatgattac ttttttcttt
     4081 accaataaaa aaatgagtaa agaagccgtg atttttttct ttggagacca aaaagctacc
     4141 tttattgaaa cgttagtaac atctcttcag cttaatttga attacatttt ttcaattatt
     4201 attgttatat cgttattgaa tttttttaaa aaaagaaatt caggaaaggt gtttaactct
     4261 aatggaaatg tgtactacaa ctatttttat tttgtatttt gggtagcagc aactttactt
     4321 ggttatgata aaattcagat tgcaggaatt cctatacaca tgcaatataa gttggttttg
     4381 agtggaattt tttctgaggt gttgccagat atatacgatg atcattacga cagtgatgga
     4441 acatgtaaag taagcattga aaaagaaaat ttcgatgaca tcgatggata tgactctgtg
     4501 aacttactga ttattgatac ttacgatata aaaatgagtg aattatctat ggaaaatcaa
     4561 acatatccta cgataattgt tagagggaat tcaatagatg gagttcgtaa agttaataga
     4621 tctttaatct tggaaataaa aaaaacaatg gatgaaattc aaaaaagtga ctttaaaaaa
     4681 gtatttgttg cttcaacatc taatccaaaa aatagtatca atattattaa ttcatcgttt
     4741 agatttttcg gtcgatcacg taggtttaaa ctttatgttc ttcaaaagga ttatgcaagc
     4801 aatggaaagt atagtaagaa atatagaatt tttatttaag aaggaatttt tccttctttt
     4861 tattttggag gaaactatga aaaaaataaa aagctacacc agtatttggg cagttgaaaa
     4921 agtcatttat gcgattaatg actttcagct gccttttcct gtgaccttta accagatggc
     4981 ttggtttgtt ttatccttga tgtttgtcat tgtatttgcc catgtgcctc cgctatccat
     5041 gatagagggg gcttttttaa aatacttggg cattccgata gcggtcactt ggttcatgtc
     5101 gcaaaagacc tttgacggta agaaaccatt gggattttta cgatccttta tcagctatca
     5161 cttacggttt aaggtcactt ttgcaggaaa gaaagttaaa gaacagaaga aacgatggga
     5221 cgaaccaatc accttggtaa ggagtgtgaa ttatgtaccc gattaagtat attgaaaata
     5281 atcttgtctt taaccaagaa ggcgagtgtt ttgcttacta tgaattagtg ccttacaatt
     5341 actccttttt gtcacctgaa cagaagtatc aagtacatga taactttcgc caattaattg
     5401 cgcaaaatcg tgaaggtaag atccatgcct tacaaattgc cacagaaagt agtattcgtg
     5461 ccacacaaga acgttcgaaa aaggaaatta ccggtagact caaagaagta gcaaagcaac
     5521 gaattgattt acagacagat gcattagtat ccatgattgg cgatagtcag attgattatc
     5581 gcttctttat tggctttaaa ctgatttcga cagatgaaga ggtcaatcta aagaatctca
     5641 ggaaatcttt cttttctgga cttcaagaat ttgtctatgg agtgaatcat catttgatgg
     5701 gtgattttgt ttccttatcc aatgaagaaa ttcgtcgcta taccaagctt gaaaaactaa
     5761 tggaaagtaa attggctcga agatttaaag tcaaacgagt aacgccgagt gacttgacgt
     5821 atttaattga acatatctat ggtgaaaaag gcacaccgtt tgaagaatat gaattccagc
     5881 taccaaagaa aaagctgaaa tcagaaacgc tggtgaaacg ttacgactta ttacgtccca
     5941 ctcgttgttt gattgaagaa aagcctcggt acttacggat ggaacatgaa aatcatgagt
     6001 cttatgtagc gtacttaaca attaatacca ttgtagggga aatggagttt ccttcctcgg
     6061 aacttttcta ttaccagcaa caacaattta cgtttccgat tgatacttca atgaatgtgg
     6121 aaattgtgac gaataaaaag gccctagcta ccgttcgtaa taagaaaaag gaattaaaag
     6181 atttagacaa ccatgcctat caatctgata atgaaacgaa ctccaatgta ttagatgctt
     6241 tggattccgt ggatgaattg gaaaccacct tggatcagtc gaaagaatcc atgtataaac
     6301 taagctatgt ggttcgagtg agtgctaaat cagtggatga attaaaacgc cgctgtgatg
     6361 aagtgctcga tttttatgac gataccaatg taaaactagt ccggcccttt ggcgatatga
     6421 tgggtctaca caaagaattc ttaccctcaa gtaagcgata catgaatgac tatattcaat
     6481 atgttacgtc tgactttttg gcgggacttg gttttggggc tactcaaatg ctaggagaat
     6541 tagaaggcat ttactttggc tataacgtag atacggggcg caatgtttac ctgaaaccgg
     6601 cattggcatc acaaggggtc aaaggctcag taaccaatgc tttggcatct gctttccttg
     6661 gttcgttagg tggggggaaa tcttttagca acaatctctt agtctattat gccgtccttt
     6721 ttggtgggca agctgtgatt gtcgatccaa aaggagaaag aggcggttgg aaagaaacgt
     6781 tgccagaaat tgcggatgaa attaatattc ttaacttgac cagtaagcca gaaaatcaag
     6841 gcttacttga tccctatgtg attatgaaga aaaagaaaga ctcagaaagt ttggcgattg
     6901 atatcctgac ctttttaaca gggatttcca gtcgggatgg ggagaagttc cctgtgttac
     6961 gcaaagccat tcgaacggtt acccaaagtg atcagcgagg attattacaa gtaatcgttg
     7021 aattgcgtaa agaggactca ccagtcgcgg aaaatatcgc ggatcacatc gagtccttta
     7081 ccgattatga ttttgcccat ttgcttttct cagatggttc gattacccag tcgattagtt
     7141 tagataagca gttaaatatc attcaagtag cggatttggt tttaccagat gcagaaacca
     7201 cttttgaaga atataccacc atggaattgc tgagtgtttc catgctaatt gttatttcca
     7261 cttttgcctt ggactttatc catagcaacc gagaaatttt caagattgtg gacctggatg
     7321 aggcttggag ttttcttcaa gtagctcaag gaaaaacgtt atcgaataaa ttagtgcgtg
     7381 ctggtcgggc catgaatgcc ggtgtttact tcgtcacgca aaacagtgat gacttgttgg
     7441 atgaacgttt gaagaataat attgggctaa agtttgcatt tcgcagtacc gatattcatg
     7501 aaatcaagaa aaccttggaa ttctttggat tagataaaga agatgaaagt aatcaaaaat
     7561 gcatgcgaga tttggaaaat gggcaatgct tgatgcagga tttgtatggt cgagtaggtg
     7621 tggtccaagt tcatccagtc tttgaagaac ttttccatgc ttttgatacc cgtccgccag
     7681 ttcaagagag aatggagtga ggagatgaaa aagaaacttt ttcgtatctt tggaattgtt
     7741 ttaatcagtt tggtcactct gataatactt ttgagcttag ttggaacggt agctgaggca
     7801 acaggtctgg ttgatgatac agttggagcc ggaaatcttt attcccaata ttcacttggt
     7861 aactatcagt tggatttctt tgtggatagt tcgtgggatt ggctgccttg gaattggggt
     7921 gatggcctag ggaagagcgt aatgtatgga ctttatgcca ttactaattt tatttggaca
     7981 gttagtctct acttatcaaa tgccacgggc tacgtggttc aagaagccta taagctggat
     8041 ttcatttcag atacggctga aagtattggg aagaatatcc aaaccttagc cggcattacc
     8101 gaaaatggac tccagacctc gggcttttac tttgggtttc tattattgat gattttagct
     8161 ctaggagttt atgtggcgta tacaggatta ttaaaacgag aaactaccaa agcagtccgg
     8221 gctgtcatta attttgtgat gatttttcta ctttcgggtt cgttcattgc gtatgcgccc
     8281 acctatatca caaaaatcaa tgattttagc tcagatgtca gcgaagcggc tcttagcctt
     8341 ggaaccgaga ttgtcgtgcc gaattcagaa agccaaggaa aggacagtgt ggatttaatc
     8401 cgagatagct tgttttccat tcaagtccaa caaccgtggt tactcttgca atttgatgat
     8461 tccaatatag aagaagttgg cgaagatcgt gttaacaaaa tcctgtctgt aagtccagat
     8521 gaaaataaag gcaaggatcg ggaagaagcg gtcaaagcgg agattgaaga taatgacaat
     8581 gcgaatctca gtattaccaa gaccatgaac cgcttaggga ccgtggtctt tttggtgttg
     8641 ttcaatattg gcatatcctt ctttgtcttt ctcctaacag ggattatgtt gttctcgcaa
     8701 attctcttta ttatctttgc gatgtttttg ccgattagtt tcttattgtc catgttgccg
     8761 acttatgaga gtttgggcaa gaaggcgatt attcgcttgt tcaatacgat tatgatgcga
     8821 gcaggagtta ctttggtgat tacgactgca tttagcatct ccacgatgtt tttcaatatt
     8881 tcggcaacct atccattttt catggttgct tttctgcaaa ttgtgacctt tgccggtatc
     8941 tatttcaaat tgggcgacat tatgaatatg ttcaatctcc agagtaatga tagccaatca
     9001 atgggaagac gggtcatgcg aaaaccgcaa atgttaatga atcgaacatt gcggcaactc
     9061 aatcgaaatg tgggacggac tttggctttt ggtggtggtg cagcagttgg aaataagttg
     9121 gcaaaggaac aatcaaaatc caaatttaag ccatccggtt cttctctacg aaagaattca
     9181 cgattaccga acgatcacga gataccatct gacttgaata aggaaaagcc aattccaaat
     9241 aataagaaac aatcccgaat gaatttggcc gggagaaaag tcggcaaggt tctggatacc
     9301 caagctttgg tgaaagataa gaccaaacaa gtaaaggatg gagtacggaa tacgccaacc
     9361 aacttgaaat acaacttcca taaaggaatt gaaaaaacga aaaaagcacc ggaagagttt
     9421 aagcgaggac tggtccaaga aaaagcaaat cgaggagagt tgcgagaaaa acaacaacaa
     9481 cgtagagacg aaaaaatagc tgaaaagcgc aaagtattga acgaaatagg gaatcctcat
     9541 gggaaaagaa gggaaaatgt tccgataaaa gcaaaggtcc aatctcaaaa ggatagagca
     9601 ccaaagcgga ttgtgagagc aaattcgaat cccgaaatca aacgaaaatt cgctagccaa
     9661 gaaatcattt caaaaggaat aaaccaatcc tcaagtaaga aaaattcttt tcaacaagtg
     9721 aaacggcggt ctactctgtc caaacgaacc aatcgaaaag tacaaaaaag tttgaaccct
     9781 catcccaaac caaggtcagg tgagaaaaaa tgaacgtaaa gaaattaagt gccatttcgt
     9841 taactttcct aacaatctct tttatgggta tcttgatgtg tgtgggctta ttgtttggtg
     9901 aagacagtga aggaagtagt agttcaggtg ctcctcctgg ggggagtagt gtctcagaag
     9961 aggttttgaa acaccgtgca atggtggaaa aatatgccaa agaatcggat ataacagagt
    10021 atgttccgat tcttttagcg attattcaag tggaatctgg tggcacaatg gaagatgtga
    10081 tgcaaagttc tgagtcagcc ggtttaccac cgaattcatt aagtaccgag gcatccatca
    10141 aacaaggatg tctctatttt gcgtctttgg ttaagcgttc caaagaatta ggttgtgatc
    10201 aagatagtat cattcaggcc tataactttg gcggtggtta tttggactat gtggcaaaaa
    10261 acgggaagaa gcatagtttt gctttagctg aatctttttc aaaggaaaaa tcttgcggtc
    10321 aaaaaatcga ttaccctaat cccatcgcga ataaggaaaa tggtggatgg cgctataact
    10381 atggcaatat gttttattgc ttattggtga agcaatacct aactacaaca cagtttgacg
    10441 ataaaacagt ccaagggatt tttgacgagg cgtttaagta tgaaggaacg gcttatgtat
    10501 ttggtgggtc aagacccgaa acaggatttg attgtagtgg cttgactcaa tggtgttatg
    10561 ccaaagttgg tctgaaactt cctcgggtgg ctcaagatca gtatgatgcc atgacccata
    10621 ttgacctaaa agatgccaaa ccaggggatt tggttttctt tcattcgact tatgatgccg
    10681 gtacttatgt gacccatgtc ggtatttatg caggggagaa tcgcatgttt catgcaggag
    10741 atccagtagg ctggacgaat ctaacagaaa gctattggca gcagcatctt atcggtgctg
    10801 gacggtacaa ataagagaaa agaggaaaaa gaatgaagat aaaaattgaa cggaatcaaa
    10861 aggaaaagag cctaaaaaag aaaaaagaac gagtcgtatc cgtgggtaag catcgaaaaa
    10921 tggtgcttgc cctttggctt ttattatctt gtagtcttac ttttggcgtt tataaaaatt
    10981 tcaccgccat tgatcaacat acaactcatg aaaaagtggt tattaaagag aaagtagtga
    11041 atacttctgg tattgaaaac tttacgaagg atttttcgaa agaatatttt tcgtggaaaa
    11101 ataacaaaga agcaattgaa aagagaatga acaacctcga acagtacctt acagaagaag
    11161 gtttcgcttt aagtcaagat atggttcgag cagatattcc cactaattcc gaagttcagt
    11221 cagtaaaagt cttagatgtt gaaaagagct cggaggaatt tgtcgtttca tttttagtgg
    11281 agcaaaagat tacagaggga aaaaaggcac aatcgatttc ttctgcatac cgagtgacga
    11341 tctttgaaga tgaaaacagg aattatatag taacgagttt gccaactatg attgcaaaac
    11401 ctgatagggc aaaatacaag acaaaacaag tagaaaatga ttcgaaaatt gatgccaaaa
    11461 ctacagaaga aatcgctgag tttttggaaa cgttctttaa attatatcca tcagcttctg
    11521 aaaaagaatt ggagtattat gttgaaaatg atgcgatgcg accaatcaat attgacttaa
    11581 agttttcgaa gttagaggat ccagtttacc tacaaaacgg aaaaaaaatt ctagtgagta
    11641 caaagataaa ataccttgat aatgtatcaa aaaccactag ctactttcag tacaaattaa
    11701 tattacaaaa aaatggaaac tggaaaatca ttgatgttaa ataataatga ctcaatctaa
    11761 atttatgaga agttataagt agtttaactt atttcaggca gaagtcgccc atcctctata
    11821 ggtgggtgat gaatgccgtt cggtaactcg aaaaccgaca attaagacat agtgcctttt
    11881 tattttaatc catgtcataa tcagtctata taagaaatgg ttgatatacc atggaattag
    11941 atacaaataa tcattcatta tttcttcttt gttaccacct tattttagta gtgaaacatt
    12001 gtaaacatct cattgatgat gtaatgttag cttatgcaaa aaactatctt tgaaagaata
    12061 tcagaatcac atcacattac cttagttgag tgaaatcgtg ataaagacta ctttcatatt
    12121 atgctcaaag cacaacctaa aaccgaatta acaaaattca gcaatgccta agctcatgtc
    12181 tgataaaatg agactttcta agggtcaaaa atttcctttg aatacgatca ttagcttaga
    12241 taaaagtaaa aaccttagaa ctacggggat agcttggtta ataagagata tcgctgttaa
    12301 taaagacata agcttgcaag tatgctctat tcccaagaat ctcccacttc aagcactaag
    12361 gatcgttaaa ttcagctaag tggcaagtag ttcactagac gataattatc tccatatttc
    12421 aatctcagct tttccatttt tcatagcaaa ttccgttatt tttatagcat ctaaaagatt
    12481 cagcttttcc actgatcttt caccactttt cagctttttt attgtgctta aattgatacc
    12541 agtacctttt gatattgaat tggaagtaaa atcattaagg gtttttttta ttaaattcat
    12601 atccgcaatt ccagcgttca taagaatgaa gattctcctc tcatagacta ctttgaaaat
    12661 ggtactacag tggctgttta tatatttcag taggttactg tagtggtcat tttattataa
    12721 ttagaaaagg aacctaaatc aaaaatgcta aagaaaattt tcatagtatt aaacctatca
    12781 tgactaaagc cacggaattc ttgataaaag agtatacttg caagcctatg tctttactaa
    12841 cagcgatatc tcttattaac caatctatcc ccgttgttcc taaggttctt gatgttttct
    12901 aaggcaatgc ttgtatacat agcccttgat caaaatattg atactaacgt tgacgtttcg
    12961 atcatgatgt gtacggcaaa taggacaagt ccattctcga attgtgaatg acttcttacc
    13021 atctgtgtgt ccacattctg aaccaatttg actagacgga aatcatttat ctatcttgat
    13081 gatttttttg acagaagtat cgaaaatctt tacataatct gggtgttgca ttcttatgca
    13141 gatgagagcg ttagctccgt attcataacc agttgcctct aaaacgtagt ccatcggtgg
    13201 atacctagct tgcggttgcc agtggctttc gcagtcttct tgtaatcaaa tcgagcatct
    13261 aattgagtac tagtattttg aagataatac ttgagttttt cggttttcag aagttcactt
    13321 catcttccat catccatgga aatcccactc acccatacgc aggcctccaa aaattcttca
    13381 acaatggttt ttcagacatt tttctgaata cctccttgtt ttttgatacg ccagatagtc
    13441 aggaaaaagg caacaaaaca caaaacaaag ctgcctaaaa aggtaatctt cattccatat
    13501 aaaaagacat ccggccgatc tgcgatatag ttagttactc gttggcccat cttagcagac
    13561 atcgcttgat ataagaccgt cgtagaaagc gaaatcccga tgaccatccc caaatttcgg
    13621 gcaaaggaat tcatggagcc tgccagtccc aaattttcga tagtgacaga actcataacc
    13681 atcgtattat ttggggactg gaacaaggca ttgccggcac ccatgatggc agtagcgaca
    13741 atgtacagcc acaaaggaga gtccactgac aaaaacatat acatgacttg ggttaccgac
    13801 aataaaccca agcctactaa tacaaaaatc tccgggccaa cccggtcggt caggtatccg
    13861 cttaaaggcg ccccgacgac catcatcaag ggaaagacca tcatcaacag tccggcatag
    13921 ctaggactca acccgcgagc attttgcaga taaaaaggaa tcactacatt aacaaagaag
    13981 ttggaagaaa aaatcaacaa ggctgtaatc aagctcatgg taaaaatctt gttcttaaaa
    14041 ataccgaaag aaatcaatgg agaggctgct ttcttttcaa cttttaaaaa gctgacaaag
    14101 gcaatcactg aaattacgaa cagtacaagg gggactggat ggaaaaagcc actttcttgc
    14161 cccaaaaaga ttgttccaaa aaggctgata atcgtagccg caatggcagc aaagccccct
    14221 ccatcgattg tatggccgct catggtgata tcttttggta agaatttttc agcaatcaac
    14281 atcgttacga ttcccaccgg tacattgatc caaaaaatgt acgcccaaga aaattgggac
    14341 aaaatcaagc cgccaagacc tggtccagca atcgagccta atgaaacaag cgcaccgatt
    14401 gctcccaatg cccgaccacg ttcatgtatg ggaaagactt ccgtgatgat tccggcattt
    14461 gtagccatag tcatactagc accaatggct tgtaccaccc gtgcgaacaa ttaaatacct
    14521 agcgaatgat tgaacccaca caacagcgag cccaagacaa agatcagcgt accaatccga
    14581 aatactttga ttttcccaca gctatcgcca attttaccaa acagcaacaa acaagaacag
    14641 acaatcatca gataaacgga cactacccat tccgcttgat tcattggtat gcccatatca
    14701 tctgagatgg taggtagtgc aatattgaca atactgccat ccaatgtgga catgaaggta
    14761 aacatagata tagcaatcaa aatccaccag cgattttttt ggacttttgc atcttcttga
    14821 taactatgca taataggatt tcctttctaa tgatcgtgta gctaagaaca atataaccta
    14881 tgtctactgc ccaacaactt ctcgcgactc tagccatagg aagcttgagt aatttcgatt
    14941 tgttattctc atgtaaccat gtaagtttga gatgctttat ttgaacttac tctatagtaa
    15001 ctagtgataa ttcgctttac tttcttctag caatttctaa aagtcgactt cattgttctt
    15061 cagattaata gtgtgagcta tctcgtgaga cgtaagaaaa tttccattct actttttccc
    15121 aattttttca tatttatggt gttgcaccta tttgttttcc aatttgcttt tcaaatacat
    15181 tgctatgata ttcaattcca taggattata ctaaatgtca atagtatgaa taatatatta
    15241 tagatgttta tatatcactg ttaattatta tccacaataa ttaatgatta cctcgatatt
    15301 tgtcaatcaa aggttgaaac aatttatttt cagcatcttt tctaacttga gctgcactct
    15361 taatagaatc atacgatcct aaatagtacc tctttttttt aaagttatat acgcaatcca
    15421 tttctcttta gaacgataaa ctcccttaac accacttgta ttatttttcg gtaattttga
    15481 cgttagacac tctattactg tcccttcaac acatgtcctt gtttcttgcc tttttaaaca
    15541 tccacaactt tgagtatgtc caaaaaataa gttactataa cttatctcac acatactgcc
    15601 acactcacat tgacaaaccc attttgtttt ccatgaacag ttattgtcca aaggatactt
    15661 tgcagtaagt aatccgaatt ttttattttc aatgtgatgc ttttttgatt tcaaacatcc
    15721 acagcttttt acatcacctt ttagaagtcg ctttgccgat actaactttt ttctcccaca
    15781 cagacattga cattcccaaa caacagttcc ttcacttcgc agtttagttg gcttgattgc
    15841 aactaactta ccaaaagttt tatttgtcaa atttttatat ttatgcctat ttttataatt
    15901 tccacatcca caacttttgg ttctacctga tagtaagttt gatcgatata caatcgtttc
    15961 attcccacag tcacataggt aacgatattt tttatatcca ttatgagtaa tctcctcaac
    16021 aacagtaagg tgaccatatc tttccttcac tgcacctacc ctcccaagaa agaagtacca
    16081 tataatttca attaactttt aataaatcag aaaaaaatat tttcaatttt tcttagtctg
    16141 attttatgac taatttataa tacaagactg aacgatacgt acacgaaatc ctcaagatag
    16201 ataaattgtt ttcatctagt aaaacctgct caaaatatga gcataaagac ggcaataata
    16261 cactcgatat tcgataatgg acttgtttta tttgtcaaac tcatcgtaat cgagatatca
    16321 attccagtct caatatcctg atcaaaggtc taagaatcca accattttct tgttactttg
    16381 tcagattgat tacctttttt agtctccgaa taataaggtt ttcggatcaa ctaacatgct
    16441 ctgagaaaga ctactatctt tgcaaatttc caaatgaagg tgtgggccag tactattccc
    16501 agtacttcct acatagccaa ttatttgtcc ttgcaaaaca ttatctccag tttttactag
    16561 gtattgttgc tggtgagcat ataacgcggt tgatccatct tcgtgttgta tcaccacgta
    16621 atttccccaa gagtagtgaa actctgcttt aacaacctta ccagctttaa ctgcatgaat
    16681 cggctcaccc tgtgtagctg ccatatcaag tcctcggtga aattcatttc cacgatttcc
    16741 aaacaaactg gttattgaat aattatgaat aggcacttgg tatccagtct tactcatttg
    16801 tggaccttta acttctttat catagttggt taaatcatag gttttaatta attcatttag
    16861 cttcaaatag tattgtgtat ctgttgcata ccgtcctgta aggaatttag ttgcttctcg
    16921 atatgtttta ctattagctt ttaatgctcc tgcataaaaa tctttatttc cactaattcc
    16981 cttagttaat aatttggcat aatcttcaaa actttcctca tagttttcat acactctaaa
    17041 ttttgattgg gtcgtataaa gcgttccatt acccaaatct tcgtttgtta ccataatgac
    17101 aaagtttcca ttataagttc cttttatccc aaataaattg taattcggtg cctgagctaa
    17161 ctggctttga ccactggcag attctaagat cgcttgtgca atcataacag aagcatataa
    17221 atcatatttc tgaccaattt ttctcgcaga ctcaccaatt ttcataataa aattttctac
    17281 agattcattt ttgtcaaaat gaatttcttc attaccagat agtattggtt gtgattggaa
    17341 agattctgtt gattctattt gctgtggtgt cgaaaaattt tttgtttgtc cttccgtttt
    17401 tggcgcttcc ttttttgaag actctatatg gtttggtctt gtcactttcg aagaagttat
    17461 ttcacttgta ctacttgaag ttgttgaaga ttcggcactt gaaatcgtac tactttcact
    17521 tgacgaagtt gttgaagact cggcatttga agtcgtacta ctttcacttg acgaagttgt
    17581 tgaagactcg gcatttgaag tcgtactact ttcacttgat gaagttgttg aagactcagc
    17641 atttgaagtc gtactacttt cacttgataa agttgtcaaa agttcactac tctctgctgt
    17701 tacatcggaa ttactcattt cagtcgtctc ggttctttct tttgcaattt tgtatgttga
    17761 attttcgctt gccaacacat ggaaaggtgt tgctataaag agttgggaca ttaatataac
    17821 tgctgatgca agttttaaag attttttcat ttgatttacc tctctttttt ctctttaata
    17881 gtgttttcta ttaaaaatta tgataaatat tttcaatgaa ttgagtaata cagtatattt
    17941 cttgatttca tttagtaacg attatgtttc aatgtttaat cctgttactg cctttaaaat
    18001 aaaattatag ggttggctaa aactaggatg aaataaataa tctaacattt tcaatttgtc
    18061 taatgtaatt tgttcttcta tagcaagaga aaacattgat aatagtccag ataaatcttc
    18121 ttgactacaa agttgtacac ctactattct ctttgtattt ttttcaaagc taattcttaa
    18181 ttttacacga ctattttttc tcaaaaaaac aggtctaatg aaatcttcta tttctatata
    18241 atctatagac tcacttaata atcgtgctgt ttttaaattc aaacctgctg cgtaaaaatt
    18301 gtaattaaat atagaaaagc catttgtaaa ttgggaacca gaaggtgtaa aatcttgatt
    18361 gtcaatatgt tgagttgcaa tataagctgt tcttagagca ttagaaatag aaaaatcaac
    18421 actacttcta ttaatggacg taaaaaaagt tgtagcacaa tccccaatag catatatatc
    18481 ttttatattt gtttgctggt attcattagt tagatatgct ccactaacat ctctcaagag
    18541 ttcctttttg ccaattggac tattagatag aaatcctata gcttgtacga ccatatccat
    18601 agagtattgg gtaccacttg tagtgactat tttttcaatc ttttttcttc ctaaatattt
    18661 tttaggactt tctgatagac aaagatgtat tccatttttt gatagtattt cttcaacttt
    18721 atctgaaaaa ctacggtcgt aataagtcga caacactcta tctgcaacat cgaaaagata
    18781 tacttctttc cctctttttc ttatggcttc agcaatttca actccaatat atcctgctcc
    18841 aataattcct acttttttaa tagtattatc atctaaagct ttaaaaatct ctttagcatt
    18901 tggaaaattc tttatcgaaa aaatattagc taaattattc cccggtacat ctaacataat
    18961 ttgctttgaa cctgtagcta gtataagttt gtcatattct tgggaaaatc ttgtgccatc
    19021 tttttttaca caaataatct ttttattttg aatatcaatt ttgtcaactg ctgtctccaa
    19081 aaacaacttt tttatcttag aatttagttc tcttctattt tgataaaaaa gatcttctaa
    19141 attgctaact tctcttttta tgaacaacga tgtcccacat tctaagtaac taatttctga
    19201 ttttttatct atcagaataa cttcataatt tgaatcacat aagtttagaa atcgagctgc
    19261 agctagcccc gaatggtttg atccaataat tactattttt ttcacttaat cacattcttt
    19321 tcccttctat gaaaagtatc ttcattttta actaacaacc tatctcccaa aataaaaaac
    19381 aaaaagagga agaaaaagat tcttcctctt ttcagagaag atttaagaaa ttgattttct
    19441 attcattctc tttttttcga tttttatacc ctaataaccc taaagttcct aacaggatta
    19501 aaccaccact aatagtaaag atattactat caatctctcc cgtcttagga taagcttttg
    19561 aggactgctt ttgaggactg cttttgcctc tatttttgtt ttgcttgcta ccttgattgt
    19621 cagtcttatt atctttacta acggcttttt cactgtcttt ttcgtttgaa gaaacaacta
    19681 cattagtagg ttcactagta ttaccagatt catcagttgc agttactatc acttttgttc
    19741 ctgcctctaa tttaggaact ttaactgtaa aattaccatt tttatcagct gtcccatggt
    19801 attgactccc gtttggtaat gtaactgtca cctctgcatt tggttcagtt ttaccagtaa
    19861 caatttggtc accctctgta ataggatcaa cttgaggttt attcggtgct tttttatctt
    19921 ctacagaaat agtaacctct acttcttctt tagatccatc tggatatgtt actactacaa
    19981 tccctttttt gtttccagct gtactaacat ctactttctc tttccaagta taagtagttc
    20041 cttttggtag atcttctttg ttcttaattc cctcagatgc gtctggctct ttatttaagt
    20101 ctgtagtcac tttttgtcct tcaggatt
//
LOCUS       DACJGB010000057        19685 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.057, whole genome shotgun sequence.
ACCESSION   DACJGB010000057 DACJGB010000000
VERSION     DACJGB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.057"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            314..1093
                     /locus_tag="IYI34_002101"
     CDS             314..1093
                     /locus_tag="IYI34_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559948.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1139..2083
                     /locus_tag="IYI34_002102"
     CDS             1139..2083
                     /locus_tag="IYI34_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0559949.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2099..2878
                     /locus_tag="IYI34_002103"
     CDS             2099..2878
                     /locus_tag="IYI34_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAR0559950.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2890..3588
                     /locus_tag="IYI34_002104"
     CDS             2890..3588
                     /locus_tag="IYI34_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAR0559951.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3616..4380
                     /locus_tag="IYI34_002105"
     CDS             3616..4380
                     /locus_tag="IYI34_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAR0559952.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4424..5875
                     /locus_tag="IYI34_002106"
     CDS             4424..5875
                     /locus_tag="IYI34_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAR0559953.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5907..7043
                     /locus_tag="IYI34_002107"
     CDS             5907..7043
                     /locus_tag="IYI34_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAR0559954.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7043..8149
                     /locus_tag="IYI34_002108"
     CDS             7043..8149
                     /locus_tag="IYI34_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAR0559955.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8155..9177
                     /locus_tag="IYI34_002109"
     CDS             8155..9177
                     /locus_tag="IYI34_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAR0559956.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9199..10254
                     /locus_tag="IYI34_002110"
     CDS             9199..10254
                     /locus_tag="IYI34_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR0559957.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10872..12107
                     /locus_tag="IYI34_002111"
     CDS             10872..12107
                     /locus_tag="IYI34_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559958.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12191..13279
                     /locus_tag="IYI34_002112"
     CDS             12191..13279
                     /locus_tag="IYI34_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559959.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13276..14697
                     /locus_tag="IYI34_002113"
     CDS             13276..14697
                     /locus_tag="IYI34_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAR0559960.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14790..15449
                     /locus_tag="IYI34_002114"
     CDS             14790..15449
                     /locus_tag="IYI34_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAR0559961.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            15468..17051
                     /locus_tag="IYI34_002115"
     CDS             15468..17051
                     /locus_tag="IYI34_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAR0559962.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17186..17764
                     /locus_tag="IYI34_002116"
     CDS             17186..17764
                     /locus_tag="IYI34_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559963.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            17710..18228
                     /locus_tag="IYI34_002117"
     CDS             17710..18228
                     /locus_tag="IYI34_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559964.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            18236..19276
                     /locus_tag="IYI34_002118"
     CDS             18236..19276
                     /locus_tag="IYI34_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAR0559965.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            19525..>19685
                     /locus_tag="IYI34_002119"
     CDS             19525..>19685
                     /locus_tag="IYI34_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR0559966.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         7182 a         2485 c         3842 g         6176 t
ORIGIN      
        1 aactattatt aattcaatgt taattttttt gttattctat tttttgtgta acttttaaaa
       61 acacctatga aaattatgga aataaaaatg aaatcgatta atttttatct tattaatgtt
      121 ttatttactt ttaaatcgta aattgcccta gtcaagaaaa cagtttgtgg tatgattata
      181 tagggaaaac ttgtttacaa atatttctgt gaatatatta gtttaattat gtttttttaa
      241 ataaaaagct gtcattttta tgtaagtgaa ttatatctta atttatctat catcaatact
      301 taggagtgaa tctatggaaa agccattcgt caagtcattg actgctgtgg cggtaggtgc
      361 aggtgctatt gcgatctgtt tgtttggata ccatatcaat aatcagcggc aacatcagca
      421 acggatcaat tatgcagaat ctgccatcac caatcaaaaa gacaccttga cttctttatc
      481 aaaagaagta gacaagctgt atagtacaaa agaaaaaatt tttctaaacc ctgaaataac
      541 agaagaaaca atcacaagtt tgtcaaataa gctatcctct gtcaaacttt cagcagatga
      601 ttacgacatc aaagaatcag aattgcctaa agaagctgtt gcaatccaag atgagaagaa
      661 agcaatcgtg acacagttag atagtgcaga atcaaaactg aagatccaga cagctgtcaa
      721 cgatttattt actaaaaatg tatcaaactg gcagcaagct gtagacgatg tcatcatcaa
      781 agaaaaggtg acaagtgcag atgtcgctcg tgtacgtgaa aatatgagct tttttaaaga
      841 tagtccttgg aaaacggttg ttatgcaata tctaggattc gctgatattc agatcgcgca
      901 agtcacgcaa ctagatcagt tattcgatac gatgttaaag gatggtcaag tgactgccac
      961 cgctacttac gatcaatatt tgacggtgct cagtcaaata gagcagatcc ggaatgaaaa
     1021 aataagcgca gcatacgcaa caaaagcaca aactgttgct gagcaaatgg gctatagcaa
     1081 tataagctat tgacagttaa aacgttgatt caagcaaggt tagcaaacgg aggaaactat
     1141 ggcaagacga agaagaaaat caagagggaa aaaaatattt ctgattattt tagcagttct
     1201 tatcgtatta gtattagcaa ttgtaggagt aggagcaaaa ttatatctgg atctctcaca
     1261 ttctattgag aaaacttacg aatcagtgga acgaacccaa ggagaacgaa cgaaaccagt
     1321 tgatctggac aaacaagaat ctttctctgt tctattgatg gggattgata ctggagattt
     1381 aggtcgggtc gatcaaggaa gatcagatac gatgatggtt gccacagtca gtcctgaaga
     1441 caaacagaca acgatcgtca gcattcctcg tgatacgtat gtcgacatcg taggtcgtga
     1501 cacaaaagac aagatcaacc acgcttacgc attcggcgga gctgccatgg cgatggatac
     1561 cgtacaaaaa tatgtagaca tcccaatcga tcactatgtc tctatcaata tgaaagggct
     1621 aaaggaactc gttgatgcag ttggtggtat cgaagtcaat aatgacctca ctttctcaca
     1681 agatggctat gactttccaa tcggtaaaat tacattgaac ggggatcagg cattatctta
     1741 ttctcgtatg cgttatgaag acccaaatgg tgattacggt agacaagggc gccaaagaaa
     1801 gatcgtagaa ggcgtagcga aaaaagtatt gagtctaaac ggtgtcagca actatcaatc
     1861 cgttttgaat gctctagagt cgaatatgaa aacagacatg aactttgatg aaatgaaaaa
     1921 aattgcttta gattatcggg aggcatttgg tacgattaag caagatcagc tgcaaggaga
     1981 aggcttcatg caagataata tctcctatca aaaagtcagt gatcaagaac ttgatcgtgt
     2041 gaaaaaagaa ttaaaagatc aaatgaatct agagaataaa taggaaagtg gagaaaaaat
     2101 ggaagagaca atcagcttga aagaaatttt tgaaacatta cgcaaacact tggtaataat
     2161 catcatctca atgtttgctg gcttggccat ctcaggaatc gtaacatttt tcatcttgac
     2221 accaaaatac agttcacaag cacaattgat cgtgacactg ccacaatcag acactgcaaa
     2281 tgtcaatgat gtcaatacga acctgcaaat gatcaatacg tataaagata tgatcgtcag
     2341 tgatttagtg ctcaatgaag tcaaagatcg tttagaaaca gaagacaatg tgaaaatgac
     2401 tgctgaacaa atcaaagatg ccatctcagt taatcaggca gaaaattcac aaatgttctc
     2461 gattcaggca ataagtacga atgcagtggc tgctcaacag atcgctaaca caacttcaca
     2521 agttttccaa gagaacgcaa aagatgtgat gaatgttgat aaaatctcga tcatttcagg
     2581 tgcagttgcc aatacgacac ctgtttcgcc aaataataaa ttgaatttaa tgactggctt
     2641 aatgttagga atgatggtag gtgtaggtct ggcattctta ttggaattac tcgatcggac
     2701 agtgaaagat gaaaaatatg tgacagatac attaggattc ccaatcttag gaacagttcc
     2761 agaaatgtca gcgaaagaat tgaatgctgg tatccgtaaa aaaggaccag ctcagacaca
     2821 accaaaagct acaaacgata aaacagcgcc atcacgcaga agccgctcaa gagtctagga
     2881 ggaaagaaaa tggcacgaac acagaaacaa aaagcagccc aaactcatgc agtcagtttg
     2941 gtcacactca ccaatccctc ttcaccaatc gctgaacaat atcgtacgat tcggacgaat
     3001 atccagtttg cctccgcagc tggtcagcaa atcaaaacga ttgttgtgac ttcatcagga
     3061 cctggagaag ggaaatcgac cactgcagca aacattgccg ttgtttttgc caaatctggt
     3121 caacgggtgc tgctagtcga tgcagactta cgtaagcccg tcatctataa aacgttcaag
     3181 ttgaacaacg caagcggatt aagtactgtt cttagttcat caggaagtgt agcagatgag
     3241 atccaacgaa caccagtcga caatttgtcg attctgccaa gtggacctaa accaccaaat
     3301 ccatcagaac tactaagttc gccaagaatg gatcagattt tagcagaagc aagacaacta
     3361 tttgatgtag tgatcttcga catgccgcca gtcgttgccg taacagatgc acagatcatg
     3421 tcttcaaaaa cggatggcac cttattggtc gtacgggaaa atacgtcacg aaaagaatca
     3481 ttgactaaag caaaagaact gttagatatg gtacaagcac gagtcttagg tgtcgtatac
     3541 aacggagcag aacacagcaa agatgctggt tattactacg gaaattaagg aataagaaga
     3601 gaggagacaa gagaaatgat cgatctacat tgccatatcc taccaggaat cgatgacggg
     3661 gcagaaaact tagaagcaag tattgcaatg gctgaaaaag cgattcagca aggcattacg
     3721 catattttat gcacaccaca ccacaataat ggaaaataca gcaatgaaaa atcacaagtc
     3781 atttctctag tcgcttcgtt acaagccgaa ttagagaaaa gacagttgcc tttgacgtta
     3841 ttggaaggac aagaagtacg tattaccgga acgctcatcg aagacatcca ccgagatgaa
     3901 attttattta cagatttaga tgatacatat cttctgatcg aatttccgac actggaagtg
     3961 ccgttatatg cagaacggct tttttttgag ttgttagaac taggaaagat accagtgatc
     4021 gtccatccag aacgcaacgc gcattttcga gaagacccaa atcatttgct tccgttccta
     4081 gatatgggat gtttggccca acttactgcg ccaagttatg ttggtgtatt tggcaaagat
     4141 atccagaaaa cagccaaaac aatggtcaaa cataatctcg tgcagatggc tgcttctgat
     4201 gcacacgggg tctctaagcg caccttttac ttaaaagagt gctatgagca gattgccaag
     4261 gagtttggta aggaaaaagt agtacagatg aaacaagtcg caaaggatct gatcaacggg
     4321 gatgagatca attatccaat ctatgaagag gtaaaaaaga agaagtttgg tttattttag
     4381 ttctttattc gtagataaaa aggaaatgag gagagagtga gagatgtata gaaaaaactc
     4441 aagtggttgg tctaaacata ttgattttat tttattagac ttattttgtg tccagcttgc
     4501 tttttatata tcatatgtaa tgcgacaggg tgactggaat ccgtatgtga ttcctattta
     4561 tcgccacatg gcaattttta tagaacttga gaatcttacg attatatttt tatttgaggc
     4621 atataaaaat gttttaaaaa gaggatatta taaagaggct gtagcttcta taaagcaatc
     4681 ttttatgctg ttacttgtgt gcagtttata ccttattaca atgcaggatg gaaatgatta
     4741 ttccagagtt tctttattta taatgggggt ggtgtatgga ttattaacat atataattcg
     4801 tatactttgg aaagggttgt tgcatcaccg catgaaaaac ggtgaagatg tatcgttagt
     4861 tattgtaaca tctcaaaaga ttgcaaaaaa tgtagttcgt aatcttaaag agaagaatta
     4921 tggcatgtat aaacttagtg gtgtgattgt agtagacaga gatatgtatg gaactaccat
     4981 cggaggtgtc ccagtagttg caaatgctga taatgcagcg gaatatcttt tacagaattg
     5041 ggtagatgaa gtattcatca atgttgacga aagtgttcca tatcccaaag aattaatcgc
     5101 cagatgttcg gaaatgggac ttgctgtaca tatgaatctt acaaaagtgt ctgatagtac
     5161 acgtggaaaa caatttattg gccgagttgg tgattatact gttattacat ctagtattaa
     5221 tgttatgacg atgaggcaag catttataaa gagaacgatt gacattcttg ctggaattat
     5281 tggatgtatt gcaacaggtg ttatttttat cttcgtagca ccagttattt acatcaaatc
     5341 accagggccg attttctttg cacaagaacg tattggtcaa aatggtaaga catttaagat
     5401 gtataagttc cgtagtatgt atatggatgc agaagaacgc aaagcagagt tgatgaaaga
     5461 aaataagatg agtaataatc tcatgtttaa gatggatttt gacccaagaa tcattggcaa
     5521 taaaatactt cctgatggaa caaagaagac aggaattgga caatttattc gatctacatc
     5581 attggatgag tttccacaat tttataatgt cctaatggga aaaatgagcc tggtggggtt
     5641 tagaccttgc ttgaagtcag aatatgagaa gtatgatttt catcatcgtg cgagaattgc
     5701 gatgaaacct gggattactg gaatgtggca ggtgagtggt agaagtgata tcacagactt
     5761 cgaagaggtt gtaaaacttg atgcagaata tatcaggaat tggagtatgg gattagattt
     5821 tagaattttg tttaggacag tcaaaactgt gcttcataga aacggttcca tgtaagataa
     5881 agatactata gaaggagtaa aacgtgatgg caagaaaaaa attaaaaatc tgtatgctag
     5941 gtcataaaag aattccatca agagaaggtg gaattgagat agtggtagaa gaattggcaa
     6001 cgcgaatggt tgcacttggg catgaagtga cttgctataa tcgaagtggt catcatgtta
     6061 gcggaaagga atttgaccag cataaaaata aagaatataa aggtgtaaaa ttaaagacta
     6121 tttttacatt aaatataaaa ggaattgcag caatgagttc ttcagtattt ggtggaatta
     6181 gagcagcatt cggaaaatat gatattgttc attttcatgc agaaggacca tgtgctatgt
     6241 tgtggttgcc aaaacttttt gggaaaagat gtgttgcaac aattcatggt ctagatcatc
     6301 aaagagaaaa atggaacaag cttgctagta catacatcat gttaggggaa aagtgtgctg
     6361 taaaatttgc agatgaaata attgtactta gtgaaagcgt ccaaaattat tttgaagata
     6421 tatatggaag aaaaacaagg tttataccaa atggcgttaa aaaaattgaa ataaaatcag
     6481 caggattgat tacagaaaag tatggattaa caaaagattc ttatattttg tttttggggc
     6541 gattggttcc cgaaaaagga attcggtatc taattgaggc atttaaagat gttcagacag
     6601 ataaaaaact tataatagcc ggaggaagct ctgatacaga tgaatttgca aatgaactaa
     6661 aggaattagc aaaaggtgat gagcgaatta tatttactgg atttgtacaa ggacaggaat
     6721 tagaagaatt atatagtaat gcgtatatct atacacttcc aagtgattta gaaggaatgc
     6781 cattaagttt gttagaagca atgagttatg ggaactgttg tatagtttca aatatctcag
     6841 agtgtacgga agtagtagaa gacaaggcta tgatttttaa gaaatcggat gtaagtgatt
     6901 taaagataag attagaagag gcatgcaatc aatcggaaat ggttaaagtt ttgaagaatc
     6961 aagctactga gtttatttgt agtaaatata attgggataa aatcgtacaa gaaacattaa
     7021 atttgtatag gggaaatata taatgattcg aatattacag tgtgtgaatg atatgcatcg
     7081 tgcagggttg gagacgatgc taatgaacta ttataggaat atagatcgaa ctaaaattca
     7141 gtttgatttt ttgacacatc gtccatataa aagtgattat gatgaggaaa taataagtct
     7201 tggaggaaag gtctattatg caccgagact ttatccacaa aattatccta aatattttaa
     7261 atggatggaa caatttttta aggatcatcc agaatatgag attgttcatt ctcatattga
     7321 tgcgatgagt tatttacctt taaaagctgc aaaaaaagct gggattccga tacggatagc
     7381 acatagccat aatacatcga tagatagaga ttttaaatat cttttaaagc aatattatcg
     7441 atgtaagtta acgtctgttt tgacacatga attttcatgt ggaaaagtag caggtgattt
     7501 tttgtttcga aatgataaat tcactttgat tccaaatgca gtggatgcta aaaaatttta
     7561 ttatgatgaa agtattagaa atgatgtacg aatgaaatta ggaatttcaa gtcagacgtt
     7621 tgtaatggga catattggaa gaatttcata ttctaaaaat catcgttttt tgattgaaat
     7681 ttttaaagaa tttcacgcta ttaagaataa ttccgtatta ttaattattg gaactggtga
     7741 tatggaagaa gaaataaaaa attatgcaaa gaaaagtggc atagatgatg atattaaatt
     7801 tttaggaaat agaaatgata tagaaaaatt ttatcaagca tttgacgtat taatgttgcc
     7861 atcattattt gaaggggttc cgttagtggg tattgaagct caatttgcag atttaccgtg
     7921 tttcttttcg gagaaagttc ctacagaagt tgctttcagt gataagacca atttcataag
     7981 tttaaatcaa tcggcaaaag aatgggcgag agagattgca aatgtagata tctctcatcg
     8041 agattcagaa cgttcgattt taataaaggc agattataat attcaaacag catacaaaat
     8101 cttagaatca aaatattatg agttatttga attaatacaa agaggttaaa gaatatgaca
     8161 cagaaacatt caagtataaa aagaattata catcgcatat ccgaagaatt gaatgcaaca
     8221 agacgaattg caaagataac atcttggaag gaagcatttg ccacgttaca agctaaaatt
     8281 gatattcaag tcatgaatcg taatggatat aatgaaagta aaaaacagaa ggaacattta
     8341 ttaaaaaaac atgaggtaat gctgaagtat tatgaaaaga ctttttcaga ttttttgaaa
     8401 acatatgatt atagtcaaaa tcaaataatt aatcaatcaa aatcaccata ttctaactgc
     8461 atttgggttt gttggtggca gggattggat caatcgccgg aaattgtaaa agcttgtgta
     8521 aaatcaataa aacgaaatgc agggaaacat actgttataa ttttaacaga agataattat
     8581 agacaatatg ttgatatacc aaaatgggtg gaagaaaaga aaaacaaagg aatcattacg
     8641 agaacaaatt attctgattt attaagatta tctttgctag caaaacatgg aggtttgtgg
     8701 ttagacgcca cattttattg tacaaaacca ataccagatg agtattttga tatcgatatt
     8761 tggtctatta ggaggcctga ttatgctcac gcatccgtag catcaggata ttttgcgggt
     8821 tattcattag catgtaatca agagtctaga tggatgtttg ctataattag agactttttt
     8881 ttaaattatt ggaaaaataa tgatatgatg gtggattatt tgatgataga ttatatgatt
     8941 gttttagcac agagaataaa tcatgaaata gcacagacat ttgataaaat acctagtaac
     9001 aatccattat gtgacgaatt gattaaaaat ttaaataata tatatgatga aaaagtatgg
     9061 aacttattaa agaaagatac tttactgttt aagttgacat ggaaacaatc ttttaaaaag
     9121 caaaaagatg ataaagttac tttttatggg atgttggtag aaaacaaatt attataaata
     9181 ttagaggtat aaaaagttat gaaacacaag gtaagtgtga tagtaccaat ttacaatgtt
     9241 gaaaaatatt taagaaaatg tgtggatagt ttattgagtc agacattaaa agagatagaa
     9301 atcgtattag ttgatgatgg ttcgccagat gcttcggggg aaatagcaga tgagtatcaa
     9361 aaaaaatatt ctaatgtaaa aacaatacat agagaaaatg gggggttagg accggcaaga
     9421 aatacaggaa ttgagaatgc gacaggagaa tatgtggctt ttcttgattc agatgattgg
     9481 gtagaaagtg atatgtatga aaaattatac ttagttgcgt ccaaagaaaa tgctgatata
     9541 gtagttagtg gacattgtga ttttgcgaat gacaaagcta tggtgaaaaa gcaacatcct
     9601 ttgagtggga atgtatatga tacgcctaat agtattaaaa atgtacgaaa aaatttattt
     9661 ggacatagtc caaaagatca agaagtagag gccttcccga tgtcagtatg tatgtctttg
     9721 tataataaaa atctgctaga taaatataga ctaagatttg aaaaaatttt atctgaagat
     9781 acaattttta acttgaatgc atatgatgtc gcaaataaaa ttgcttttac agaatataca
     9841 gattattgtt atagaaaaga agaacaagag tctattacaa aaagtttatc tccaaataag
     9901 cagacggaat ttaaagaatt tttaataact ctaaaagcta tagcatcgaa agaagattta
     9961 gaatgtcaaa tcagagcaaa acgtatggca attgattatt gtaggcttta tgtgggctta
    10021 gttgatgatg caaatttggg aattaaagag aaaagaaagt atataaaaaa atttattgaa
    10081 gatgagggat taattcaatg ttggaaggga tatcctacaa atacgctacc atttcaacaa
    10141 aaattttttc ataaaatgct tgaatgtaag tgttatacaa tattgttgtt gttaagtaga
    10201 attagaaaga taattaaaaa tatgaccaga aaaaaattgt ttataatatt ataaactaaa
    10261 agtatatcaa ataatagaaa ttaacaagtt acaaaaaaat attataaaga ataatatttt
    10321 ctaaaaagta aactatagta atgtggatta tcaccttcca acagtatata aaatgagcgt
    10381 aaaaacggct atttttgcct taacaacgat ttttttgtat acaaatacga tatgctcatt
    10441 aattattgga atttgtagtg ctgtttaaat taatgaaggt gtaattatat gagtgatcat
    10501 tttgaaaaag tttgttgaga agatgtgctt ctagtcatgt gatgggagaa aataatttaa
    10561 ctgttagagt gaatagttaa attattctgg ttatatgaaa aaagtagttt ctaaaataat
    10621 cttgcaggga ttaacttaaa gaaattgaga gaatagtttc tttgaaattt ctatatacaa
    10681 tgagaagctg aaatttactt ttatttgatg actggaaata gaaattaaat atattgtatt
    10741 gttacattaa agaaaacttg tatggaaaaa tcagtacaat accattattt aatgttcttc
    10801 acttaaagcg caaaaaatat cagtttatta aactcgtata tagaacaaat atatgtagac
    10861 ggggcgaaac gatgagtgta aaaaaaatgc tatttatggt aagtacaata attgtattaa
    10921 catcccgatt ttggggaatt aatactagtt tatctatgcg atattttatt atggcaatat
    10981 gggatttata ttttatgtta agctattttt cttatcgtga gaaaacatat aaaaaatacg
    11041 ttatcaagaa taacttttgg ttgactataa tgccgtttgt gatattttct atctatactc
    11101 taataatatg gggaattggt tcaaatgcag aatttgaaaa ttttataaag ttatgttcca
    11161 cgttgcttta tttaattctt gcatatggtt acgcatgtac agcattttac ttatttaagg
    11221 cagaaggaat tgattttatt ttttggtcag gtgttatttc atatatgagt ggttcagttt
    11281 tttatttaat tgtatcttat ggtttaaatg gtttaatttc ttatataaaa agtcttataa
    11341 caggtgaaac aaatactgca aattttgcta tggaggtaca tgatttaact tttgcgatgg
    11401 gggttttttt cctttattac gtattttttg aaaataaaaa tacaaaaaaa cataagataa
    11461 aaattatcgt atctttaatt ttaattttca tgggattgaa gagaattgaa ctgttagctt
    11521 taagtggtgc aattctagtg tattatgttt tattaaagtg gggaaaaaag attcaaatcc
    11581 gtgcattagt gtgtactatt tgtgcaactg ttatttcgtt agtgtttatt tacattatag
    11641 atacaggaat tttggcattg cttatgtcaa atttgggcat tacagatgac ggtgcaaggc
    11701 tttcatttta tctatattca tctaaatttt atgaacttgg tttaggatat gtaggaacag
    11761 gatggacatg gttttcaaaa tactttttta atctttattt atcgcacttt aggttaaatg
    11821 gtggaagaat tgcagcaagc ctacacagtg atatattaac tatttatata gaagttggat
    11881 gtatagtttt tattatatgg atgttttggt tattttttgt gaaaccgata agactaacta
    11941 aaaaatttgg attgaaaaca ggggagtgtg tattattatt aactatatat atgtttatat
    12001 tatatttgac ggataatacg ctcacttatc cagacactca aatgattttt ctcttagtac
    12061 ctatgatagt ttcgtgggtt gataatcaac aacgaatatc ggaatagata gctaaagtat
    12121 attttaatta tgaatttata attagttgta aaagttaata tttaacggac aggaagtagg
    12181 gtgaaaaaat ttgaaaaaaa ttttagtgtt agcaaataca tattatcaaa taatagtagc
    12241 aatacaaatg aaaaatacaa ttttttccga tgagtatgtt gaaattatta tttctgatca
    12301 ttctaaaaac actaaagaaa ttgcgaaaaa aatagaagaa aaaaaagtgt ttgatggctg
    12361 tcattattta gaaagcaaat cttttgatga taatattggg tattttcaaa agattaagga
    12421 ctgtcttcag atttcttgtg gtaaatcaaa tcgatatgcc tattatttga aaaactgtaa
    12481 aagtgaatat tttgatgaat ttatttgttt ttcaatggga gaggtttggt tggaaggaat
    12541 atatgcttgc ttatatcgaa ataataaaaa tgtacaggta tcaattttag aagaaggtat
    12601 tttaagctat ggagttgaca ttattccaac agcaagaaca aaagctatcg cagtactcaa
    12661 ttacttaaaa aaaaggactc cactatataa caaaagctat aatttttatt gtttttttcc
    12721 taatatatat acaggaagag agaatacaat acaaattcca ttagttacag ctgggtcaag
    12781 aaccgcattg caactttcgg atatttttaa ccctatgata gatatttata aagaaaaaaa
    12841 atatatattt tttacgtctg tttatgattt tgagggcgga gaacctgtcg gagaatatga
    12901 attagtatgc aaaatagcag acttagtagg aaaagataat ctaattgtta agacgcatcc
    12961 tagagatgta agaacaattt ataaagataa tggatttatc gaggataaaa attcatcaat
    13021 accttgggag gcgattcaat tgtcaggcga ttttaacgat aaaatattta tgacaattaa
    13081 ttcaggaagt gttatttcag gtagtacaat gtctgcaaaa cctgtacgca cgtattatat
    13141 gtataaattg tgtaacatag aaggaaatcc gtcttgcaaa aagaatgcgt atgatattga
    13201 gcgattgctt aagcaaaaaa cgatgaaaaa cattttaaag aatgtgaaaa tagcagaaag
    13261 cttagatgat atactatgag caaaactaaa tatttaatta aaaatatggg aatattaaca
    13321 ataagtaatt tttcttcaaa aattttagtt tttttgttag tccctttata tacaagtgta
    13381 ttatccacaa cagaatatgg cacatatgat ttgacaattt caacagccac attattgttt
    13441 ccaattttaa ccttaaacat tgtagatgct gtgatgcgct ttttaatgga taaagataaa
    13501 aataaaaaaa atatagcatc tatagcgatt aaagttatat ttttaagcgt gatagtcttt
    13561 ggatccgtaa tgtatttgtt aaatattaca aaattatggc aagatgttta tggattagaa
    13621 aaatatattt ttttgtatta tatatcctat gtatggaatc aatttctaat acaatttgct
    13681 aaagggttag aaaaagttaa agctatgggc atagcaggta ttatcagcac aattgtaaca
    13741 ataattgcca atattttctt cttgcttatt ctgaaatggg gattaattgg ttttttcatt
    13801 gcaaatattt tatcacagat tatttcagct acttatctgt cagtattagt gagaatatgg
    13861 gaatatatcg attttgaggc taaagatcct cagctaaaga gagaaatgct aaattactcg
    13921 attccattaa ttgcaactgt tgttggatgg tggattaata gtacagctga taggtatgtg
    13981 gtttccttta tagtaggtgt ctcggcgaat ggcttattgt ctgtggcata taaaatacct
    14041 caaataatta atactttgca gggaatattt atacaggcct ggcaaatttc tgcaattaaa
    14101 gaatatggag ataatgagac tgcaaaattt tatggaaata catttgttat gattaatttg
    14161 ttaatgtgca taacctgttc aagtcttatc atattaacta gaccgttttc acatattctt
    14221 tatgcgaaag acttttatga agcgtggaaa tatgttccat ttttattaat agctaatgtt
    14281 ttaaattgtg catcaggtct gttaggacct atcttatcag caaaaaaaga ttctaaatcc
    14341 atgatgtggt ctgcaatcat aggggcatta gttaatgtga ttatgagtgt agtccttgtt
    14401 tataagatag gggttcaagg tgccacaata gccactgtta tatgttcata tgtaatatat
    14461 gttgttcgta aacgagctgt gggaaaagat atttttataa agcattatca tattgttgtg
    14521 gaatcttggg gattattgtt tgttcaggca atcatagaaa tatattttaa aaattatgta
    14581 atagaaatta taattatgat tattttaatt attataaata tcagttatat taagcagttg
    14641 tgttatagag gaagaaacat tatttcaatg attaagagtc gagagcacaa acggtagacg
    14701 atgttatttt tataaaaaga tataatttat taatggtcga taattatgaa aggaaaaata
    14761 ttcttaaaaa aaagagtata ggatgtcgta tgaaaatagt cgctatagta ccaatgaagt
    14821 taaataacaa aagattgcca caaaaaaaca cgaaagcttt taccaatgga aaaccattgt
    14881 gttattatat actttcaaca cttttaaaag taaaacagat tgataaagtg tatgtctatt
    14941 gtagtaaccc agatattaaa gaatttatac cagaaggtgt aacattttta aagagatcta
    15001 gttccttaga tcaggataca acgagtatta cacaggtatt agccggtttc acaaaagaag
    15061 tagaggctga tgtatatgta atgacacaca caacagcacc atttattagc gaaaaatcta
    15121 ttgaaaaagg actagatgcc gtgctgacag gagaatatga ttctgcattt gctgcaaaaa
    15181 aattgcaaga tttcttatgg aaagacggtg aaccatttaa ttatgaattg gacaatattc
    15241 ctagaacaca ggatttgccg cctttatacg aagaaactag tggattttat atttataatc
    15301 gcaatgtgat gaccgagttg catagaagaa ttgggaaaaa accttgcata gttgaagtag
    15361 gtgagattga gtcagttgat attgatgaag ccgaggattt tatgattgct gatgcgattt
    15421 ataatcatct tattttacaa ggtagttaag acagggggaa ggttaggatg aacaatgtac
    15481 agattttaga ttgcacactt agagatgggg gctattgtaa caattgggaa tttggatttg
    15541 ataatacaaa aaaaattatt tctggactta tagaatcagg aattgagatt atagaatgtg
    15601 ggtttctcac aaataaaaca gtatacaata aaaatgttac aaaatatacg accttagaag
    15661 aattagcaca gataattcct gaaaaaagag aaggaaaatt atttgtagca atgatgaact
    15721 atggcgaata taatatagag gatcttccag attatgatgg ttcatctatt gatggaatta
    15781 ggattgcgtt tcataaaaaa aattgcaatg aagcgttgaa attatgtcgt gagataaaga
    15841 aaaaaggcta cttagttttt gttcaagcta tggtatctct caattataca gatgaagagt
    15901 ttttagcagt aataaaaaat gtgaatcagt ttaaaccata cgctttttat attgttgata
    15961 gttttggaat gatgaaagga aaagatttaa ctcgtctttt ttacatgatt gagcataatc
    16021 tagaaaaaga tatacagata ggttttcact cacataataa catgcagctt gcatattcta
    16081 atgcacaaaa acttataacc gtacaaacaa atcgaagcct gattattgat tcttcggtgt
    16141 acggaatggg aagaggagca ggtaatctta acacagaact atttgtagaa tatttgaatg
    16201 ataatgcagg aaaaaaatat caattaaaac cattattata tataattgat gaaatactaa
    16261 atagtttcta tcagaagagt tattggggat attcattacc aaactatatt tcagcagttc
    16321 ataatgctca tcctaattat gcaagctatc tgtcatataa gaatacttta acaattgaag
    16381 caatgaatga aatattcgaa atgatggatg aagaaaaaaa ggtatcttat gataaacaat
    16441 atatagaaga tttatactac aaatatatgg aaactgggaa aatacaggag gaatatatgg
    16501 aagaactgag aaaatttctc tcagacaaaa aaattctact gattgcacca ggaaaaagtt
    16561 ctgtagatga ggtagaaaga ataaaagagt ttgttaccaa agaagaagtc gtaataatta
    16621 gtgtaaattt tgaatattct catatagata tagattacgt atttttaagt aatttaagac
    16681 gttttcatga attaccgaaa gaaagcagaa ctaaatgtat tgttacatca aatatacaag
    16741 catcggatgt atatttacgg acgagttata aaaagttgtt atctgaaagg gaagttgtaa
    16801 gagacaatgc aggattaatg gcaattagat ttttagccga tatgaatgtg gatgaaatat
    16861 tcttagctgg atttgatgga tattcacata atgaagatga gaattatggt gagcaatcca
    16921 tggaaattat aacaaaattt gcattattag atgcaatgaa cgtgcaaatg agagatgaat
    16981 tgtcagaatt agctaaaaaa gtaaaggtga catttttaac ggatccacgt tgggttcgaa
    17041 ttgataagta gtagtaataa tgtttttaaa attttgataa agagatagtg agagaataaa
    17101 aatgggtaaa gtcttaaata aaaaagaaga aaaatattta agggccgatg gggcgagatg
    17161 aaactctaat tttgaacttt taagaatcat ttctatattg tgtattgttg cttaccatgc
    17221 taatttgcgt ttaaattata atgcagacgg aattatagta catggttcat taactgctaa
    17281 ccagattttc ttgaattata ttggtatttg gggtcagcta ggagttaatt gttttatatt
    17341 tatttcggca tattttttaa gtgagaaaag aaacattcac tttgaaaatg ttttgggaat
    17401 tgtattagaa tgtatttttt atagtgtatt agtttatgga attctttgtg catgtggaat
    17461 agaaacattt tcattgaaca gctttgtaaa agaaatatta tcatttgctc taggtagata
    17521 ttggtttatt acatcctatt tagcattgta tattctaagt ccgtatttaa atatcatgct
    17581 taacaatctg gaaaaacttc agttgcaaaa aataagtatg ttattgttta taggtgtatg
    17641 ctgttatcgt atgttattta aaacatcttt gtttggggat tgtgctgttt taattgcact
    17701 atatgtcata tgttatacac tgaaaaaaac gataataata tttttgagag acacagaatc
    17761 ataggaacaa ttgtatgtat aacagttata ccacttctca tatccatagg aatacttttc
    17821 aaatttatac caaatgcaat ggttaaccag atggttcata ttggttctcc attgatggtt
    17881 atagcaagta tatttttatt ttatgtattt aaaaattttt cattttacag taagtatata
    17941 aataaacttg cgagaaccag tttaggggta tatattttac atgaaaatca ttatttcatt
    18001 gatggtatga agtctagagg cttaatatgg gatgggatat ttggattagg caaaaggttt
    18061 ttagataggg atttttggat ttggtttata attgcagttg tagttgtgtg cataatttgc
    18121 atggctatcg atgttgtaag agaaaaattt gaaagacaga tttttaaaat taagagtgta
    18181 aagaacctgt gtaaacttat gaatcatgtt ataaatatat acccataatt aataaatgag
    18241 gagaaaagaa gtgaaagtta tatcattcgg agaattatta ttgagacttg cagcacccgg
    18301 ttattcaaag ttatttcaaa gagatagttt ggagactaca ttctgcggag gggaagctaa
    18361 tgtagcggta tccttagcaa tatttggata tcaatcagaa tttataacaa agcttccgga
    18421 ttcagatgtt ggatatgcgg cagctaatgc tttgcgttat tttggagtaa atgttgataa
    18481 tattatattt ggagagggac gcatgggact gtattattta gaaaaaggag cagctcagag
    18541 accatctaaa gtaatttatg acagaaaatt ttctgcaatt tcgttagctg atagtgatga
    18601 atttaagtgg aaggagatcc taaacgatgc aaattggttt cattggacag ggataaatcc
    18661 agcattgtct gagaatctct ttgagacgtc cttagatgca tgtcaggctg ccaaaaaaag
    18721 aggtataaca atttcttgtg atttaaatta tcgatccaag ctttggacat cagaggaagc
    18781 aaaagaaaag atggaacaga tactaccatt ggttgatatt tgtatttgta atgaagaaga
    18841 tgcagaaaaa gtgctaggaa ttaaagctga gcatacaaat attaatagtg gagaattaaa
    18901 taaagatggc tatgtgcagg tggctaggga gattcacaag aagtttggat gtaggtatgt
    18961 agctacaact ctgcgtagaa gttattcggc aagtagaaat ggttggagtg caatgttata
    19021 cgaagcagaa aaagataaag cgtatttttc cacagaatac aacatacaaa ttgttgatcg
    19081 cgttggtggt ggagacagct tttctgctgg tataatatat gcgttatgtg aaaatatgtc
    19141 cggacaaaag gctattgaat ttgcgactgc atctagttgt ttaaaacata ctatagaagg
    19201 tgattttaat cgaactacaa ttgaggatgt atataattta atgaatagcg gtggaaatgg
    19261 tcgcgtgttg cgttagtcaa catataaaga taaatcacag taatttttat ggtgattatc
    19321 tgtcttagta gtgattagaa taacgtgaaa ataaattgtt gagcgtttat actgttttga
    19381 aaaataaatt taacatattg aaaaatacaa atatatacgt atattataaa gcgccgattg
    19441 taaaattaag ctagacaact aaaacagtca tttgtgctac actcaaaata gttccgacca
    19501 aagaattata aggagtgagt gcaaataacc tatacctatc ttacaacgga tgaacttgta
    19561 ataatagagt cttatttgaa aattaaccaa tctgttgcta aaactgcgca ttgcttgaat
    19621 cgttcaagtc aaacgatcca taaagtatat ctgttcttca agcaaagaaa atcagcctta
    19681 gaata
//
LOCUS       DACJGB010000058        19507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.058, whole genome shotgun sequence.
ACCESSION   DACJGB010000058 DACJGB010000000
VERSION     DACJGB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.058"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IYI34_002120"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IYI34_002120"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAR0559967.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IYI34_002121"
     CDS             complement(1173..1868)
                     /locus_tag="IYI34_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAR0559968.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IYI34_002122"
     CDS             complement(1881..2597)
                     /locus_tag="IYI34_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAR0559969.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="IYI34_002123"
     CDS             2927..3532
                     /locus_tag="IYI34_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAR0559970.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="IYI34_002124"
     CDS             3857..4180
                     /locus_tag="IYI34_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAR0559971.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="IYI34_002125"
     CDS             4362..5732
                     /locus_tag="IYI34_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAR0559972.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="IYI34_002126"
     CDS             complement(5862..7163)
                     /locus_tag="IYI34_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAR0559973.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="IYI34_002127"
     CDS             complement(7393..9384)
                     /locus_tag="IYI34_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAR0559974.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="IYI34_002128"
     CDS             complement(9528..9794)
                     /locus_tag="IYI34_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR0559975.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IYI34_002129"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IYI34_002129"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAR0559976.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IYI34_002130"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IYI34_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAR0559977.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="IYI34_002131"
     CDS             13138..13701
                     /locus_tag="IYI34_002131"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAR0559978.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="IYI34_002132"
     CDS             complement(13954..14475)
                     /locus_tag="IYI34_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAR0559979.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="IYI34_002133"
     CDS             complement(14478..15320)
                     /locus_tag="IYI34_002133"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAR0559980.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="IYI34_002134"
     CDS             complement(15313..16038)
                     /locus_tag="IYI34_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAR0559981.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="IYI34_002135"
     CDS             16214..16510
                     /locus_tag="IYI34_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0559982.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="IYI34_002136"
     CDS             17078..18670
                     /locus_tag="IYI34_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR0559983.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="IYI34_002137"
     CDS             18683..19423
                     /locus_tag="IYI34_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR0559984.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DACJGB010000059        19455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.059, whole genome shotgun sequence.
ACCESSION   DACJGB010000059 DACJGB010000000
VERSION     DACJGB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.059"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            131..3658
                     /locus_tag="IYI34_002138"
     CDS             131..3658
                     /locus_tag="IYI34_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAR0559985.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IYI34_002139"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IYI34_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAR0559986.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IYI34_002140"
     CDS             7446..7787
                     /locus_tag="IYI34_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0559987.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IYI34_002141"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IYI34_002141"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAR0559988.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="IYI34_002142"
     CDS             9144..11564
                     /locus_tag="IYI34_002142"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAR0559989.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IYI34_002143"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IYI34_002143"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAR0559990.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="IYI34_002144"
     CDS             12998..13735
                     /locus_tag="IYI34_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR0559991.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="IYI34_002145"
     CDS             13749..14564
                     /locus_tag="IYI34_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAR0559992.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="IYI34_002146"
     CDS             14577..15224
                     /locus_tag="IYI34_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR0559993.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="IYI34_002147"
     CDS             15240..15896
                     /locus_tag="IYI34_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR0559994.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="IYI34_002148"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="IYI34_002148"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAR0559995.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="IYI34_002149"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="IYI34_002149"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAR0559996.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="IYI34_002150"
     CDS             18238..18756
                     /locus_tag="IYI34_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAR0559997.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="IYI34_002151"
     CDS             18863..19363
                     /locus_tag="IYI34_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAR0559998.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagc
//
LOCUS       DACJGB010000060        18957 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.060, whole genome shotgun sequence.
ACCESSION   DACJGB010000060 DACJGB010000000
VERSION     DACJGB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.060"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(9..530)
                     /locus_tag="IYI34_002152"
     CDS             complement(9..530)
                     /locus_tag="IYI34_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAR0559999.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            729..4421
                     /gene="nifJ"
                     /locus_tag="IYI34_002153"
     CDS             729..4421
                     /gene="nifJ"
                     /locus_tag="IYI34_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAR0560000.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4610..5497
                     /locus_tag="IYI34_002154"
     CDS             4610..5497
                     /locus_tag="IYI34_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAR0560001.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5494..6663
                     /locus_tag="IYI34_002155"
     CDS             5494..6663
                     /locus_tag="IYI34_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560002.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6687..8042
                     /locus_tag="IYI34_002156"
     CDS             6687..8042
                     /locus_tag="IYI34_002156"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAR0560003.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IYI34_002157"
     CDS             complement(8114..9919)
                     /gene="glmS"
                     /locus_tag="IYI34_002157"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAR0560004.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10328..10618
                     /locus_tag="IYI34_002158"
     CDS             10328..10618
                     /locus_tag="IYI34_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560005.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10631..11656
                     /gene="sppA"
                     /locus_tag="IYI34_002159"
     CDS             10631..11656
                     /gene="sppA"
                     /locus_tag="IYI34_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAR0560006.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11659..12318
                     /locus_tag="IYI34_002160"
     CDS             11659..12318
                     /locus_tag="IYI34_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAR0560007.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12475..13407
                     /locus_tag="IYI34_002161"
     CDS             12475..13407
                     /locus_tag="IYI34_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560008.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13561..14850
                     /locus_tag="IYI34_002162"
     CDS             13561..14850
                     /locus_tag="IYI34_002162"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAR0560009.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15097..16347
                     /gene="clpX"
                     /locus_tag="IYI34_002163"
     CDS             15097..16347
                     /gene="clpX"
                     /locus_tag="IYI34_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAR0560010.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16430..17017
                     /locus_tag="IYI34_002164"
     CDS             16430..17017
                     /locus_tag="IYI34_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAR0560011.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            17382..18709
                     /locus_tag="IYI34_002165"
                     /pseudo
     CDS             17382..18709
                     /locus_tag="IYI34_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18720..18929
                     /locus_tag="IYI34_002166"
     CDS             18720..18929
                     /locus_tag="IYI34_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560012.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6212 a         3370 c         4083 g         5292 t
ORIGIN      
        1 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
       61 aagatttctt tacccaagat gacagttgga acaaatcgaa tatgtgcttg ctcagcttcg
      121 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
      181 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
      241 tgtttgatct ctttgatcgc tttttggcca tcgctgattg tgagataccg gtgcatcacg
      301 ttccctcgct gtaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
      361 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
      421 ggacaagcta agttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
      481 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
      541 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
      601 ctaaaaatta gatgaccaat tcaaatagat acatgtaaaa tatttttata aataaaagaa
      661 aaaaatggca tgttttacgt tataatggaa cagataataa gagagaatat gaacaaggat
      721 gatgaatcat gcgaaaaatg aaaacaatgg atggcaatac ggcagccgca tatatttctt
      781 atgcatttac cgagattgct gcaatatatc caataacacc cagttcgacc atggctgaat
      841 tagtagatca atggtcagca gcaggaaaag aaaacgtatt cggtcaacca gtaaaagtag
      901 tagaaatgca atccgaagca ggagcagctg gtgtggtcca tggatcattg aaaactggtg
      961 cactgacaac tacatatacc gcttcacaag ggctgctttt gatgataccg aatatgtaca
     1021 agattgctgg ggaactactg ccaagtgttt tccacgttgc tagtcgggca gtgacgacaa
     1081 atgcgttgaa tattttcgga gatcacggag acgtgatggc tgcaagacaa acaggatttg
     1141 ctatgttagc tgaaagcagt gtacaagaag tgatggattt agcacctgtt gcccatcttg
     1201 cttctattga aggagaagtc ccttttttga atttctttga tggattccgt acgagtcacg
     1261 agattcaaaa aatcgaagtt ttggaatatg aagaattagc tcctctgatc aatcaggaga
     1321 aattagcttc atttcgtaaa cgagggatga atcccaatca tccgactgtt agtggtacga
     1381 accagaatcc agacatccat tttcaacaac gggaaacgat caacccctat tatgataaat
     1441 tacctgaaat cgttcaaaaa tatatgaaag agatcaaccg attacgagga acttcttatg
     1501 acttggtgac ttattacgga gcagacgatg cggaagaagt cattgtttct atgggatcag
     1561 ttgcacagac gattgaacaa acgattgatt acttgaatgc aaacggacgg aaaacaggtt
     1621 ttttgaatat ccatctttat cgtccttttc cagtggaaac attcgtgcaa aagttgcctc
     1681 atacagtaaa aacaattgct gttcttgatc gaagtaaaga accaggggca ggtggagaac
     1741 ctctcttatt agacgttcaa agtgcgttat atgattctga acttcgcccg gctgttattg
     1801 gtggtcgcta cggattgggg tctaaagatg tgactcccga ccaaatctca gcagtttttg
     1861 atgaattgaa aaaagaccca agcatacgca aaaaacggtt taccataggg atcgtggatg
     1921 atgtgactta tcaatctttg gaaccaaagg aaagtctgga tttgacagaa ccgcaaacat
     1981 tccaggccaa gttttgggga tttggatctg acggaacagt tggagcaaat aaatcagcta
     2041 tcaagattat tggagatcac accgataaat atgcgcaagg ctatttttat tatgattcga
     2101 aaaaatctgg cggtctgact gtctctcatc ttcgttttgg agatacgccg atacgttcag
     2161 cgtatcttgt cgagcatgcg gaccttgttg cctgtcacac accagcttac ctgcatagct
     2221 atgacttggt caaaggattg aagccaggtg gcattttttt attgaatact ctttggagcg
     2281 acgaacaatt agaaacgcat ctgcccttaa aattaaaacg ttacttggct gaaaataaca
     2341 ttagattcta tacgatcaat gccatgagat tggcccaaga agtcggtttg ggacgccgga
     2401 tcaatacggc aatggaaacg gcttttttta aattagctga catcattcct tttgatgagg
     2461 tgcttccgct gctaaaagaa gaagccctga aaagctatgg acataaatca atggctatcg
     2521 tagaaaaaaa catacaagca atcgatcgga cgaccgaact gcttcatcaa gtcgcgatcc
     2581 cagaccattg gagaacgttg gagatgcctg aaaaaatcaa taaaaggaac actggttata
     2641 tacaagaaat acttgaacca gtgaatgcac aggaaggaaa tcaactctct gttggtacac
     2701 ttgtagcaaa tggaatgaca actggcgaaa tgcctttagg aacggcttct atggaaaagc
     2761 ggggcgtcgc tttagaagta cctgagtgga ttagtgatcg ctgtacgatg tgtaatgaat
     2821 gtgcctttgt ttgtcctcat gctgccatcc gtccattttt agcagatgaa gaggaaatgg
     2881 aagaagcgcc agaaggattt atcgtgagag aaatgcgagg cgcagacggc gtaaaatacc
     2941 gtatccaagt ttcagtggaa gactgtacag gttgtggtct gtgtgtagag gcttgtcccg
     3001 caaaagaaaa agctttagtg atgaagcctt atgaagaaca gaaagaacaa gctgtgaatt
     3061 gggcttttgc aatgacatta agacaaaaag caaatccagt caaaggaaag aatactgtct
     3121 tgtctacaca attcaatcaa ccgcttcttg aattctcggg cgcttgctca ggctgtggcg
     3181 aaacacctta tgtaaaatta ttgacacaaa tgttcggtga tcgaatgatg atcgcgaatg
     3241 cgacaggctg ttcttctatt tggggcggtg cttctcctgc ttctccttat acgaccaatg
     3301 aatgtggtca agggcctgca tggtcaaatt ctttattgga agataatgct gaatttggtt
     3361 atggtatgct tctagcggca cagactcgtc gcgaggcttt ggcaagttca atcaaagaag
     3421 tattgccaaa agtatcagaa gaactacgag aattgatgga agactggtta gtacacatgt
     3481 ttgagagtga aggaacgcag caaagagcag caaaactgca agctgcgatc gaaagcgaac
     3541 gatcaatttt ccctgaactg gaggatatat acaagagaaa ggatctattc gtaaaaaaca
     3601 gccaatggat gattggtgga gacggatggg cttacgacat tggttttggc gggatcgatc
     3661 atgtacttgc cagcggagca gacgtgaata tccttgtttt ggataacgaa gtctattcca
     3721 atactggcgg tcaaacctca aaagcaacac cagcttcggc aatcgctaaa ttttcggcta
     3781 gtggaaagta tgcttcaaag aaagatctag gaatgatggc gatgacatat ggaaatgttt
     3841 atgtggcgca aattgcttca ggtgccaatc aaatgcaaac gatcaaggca tttgaagaag
     3901 cagaaaagta tcctggtcct tctctgatta ttgcctatac gccttgtatc acacatggct
     3961 tgcttggcgg aatgaaagaa tcgcttaaag aagcaaaaga agctgtttct tctggttatt
     4021 ggtcattata tcgatataac cctacaatgg tcgaaaaaaa caagaaccca atgactttgg
     4081 actacaaaaa acctgatttc tctgctatgc ctgatttcat gaggaaacaa gttcgctttt
     4141 cctcgctaga taacgcaaat ccaaatcttg caaaagcttt atttgaaaag actgtccacg
     4201 acgctaaaac acgtttttac aactatgctg cactgacggg agaattagaa aaaatcaaga
     4261 aacgacttga accagaggaa tctatcgatg tacccgttaa gactagaaaa gaaaaaacga
     4321 cggaccttga agcagaagaa agaagagctg ctagaagagc tgccagagca gaacgacgta
     4381 aaaaaatgga acaagctaca aaagaaaaaa aggaaaaata acagaaaaaa tataatgaat
     4441 aattctctga agtttaaaaa tatttattgc agagaaaaat aaaacgcata cttgcaattt
     4501 tcttgaaaat tatgcgtttt tctcaagaaa aatacgagca tatcagcctc taaaattaaa
     4561 acatatgata tactagagag gaaatctcaa aagagaagag aggcttttta tgtcatttca
     4621 aatcggggaa ctttttaatt tggactattg gcagcaaatg ttttccggtg acttgttctc
     4681 tctgaatttt ttggtgaaca tacttgatat tctggttgtc tggtatcttg tatacaaact
     4741 gatccaactg ctgcgaggca caaaagcgat tcagcttctg aaaggtgtag ctatttttat
     4801 tgttatccga atcgtagcgg aaatcattgg tctgcacacc ttgtcttggc tgatgaacca
     4861 agtcattacg tacggggtga tagctgctat cgtgattttt cagccggaaa tccggagagg
     4921 actggaacat cttggacgca gttcgctttt caaacaatca aaaagcgaac agcaggaaga
     4981 tgagaagatg atcttgtctt ttgacaaagc catccaatat atgtccaagc gaaaaatcgg
     5041 tgcgttgatc actatcgaac gtcatactgg attagatgag tatattgaga cagggattgc
     5101 tttagatgca gatatcactg gggagctgct gatcaatatc tttattccta atacaccgct
     5161 gcatgacggt gcagttattg taaaagaagg aaagatcgca gtagcaagcg cctatcttcc
     5221 tctatcagaa agtatgttga tccctaaaga attcgggact cgacaccggg cagctgtcgg
     5281 tataagcgaa gtcagtgacg cgattaccat cgttgtctct gaagaaaccg gggacgtcag
     5341 catcacgtta gataatgaac tgatggctgg cttatcccag caagaatatt tggcaatctt
     5401 gcgtagagaa ctaattgctg aagagaaaac aaaaaacaag aaaaacgtga ttcaatcttt
     5461 cctagacggt atgactaagg gaggggatcg caaatgatct caaaagagaa acggacaagc
     5521 attctttaca gttttattgc attgctgttc agtattgcgc tgtacttcaa tgcaaatggc
     5581 cagtccgttc caaatacgct ttctggaaat gaagcgtata atcaaacagt tacgggggtg
     5641 ccgatcaaga tttcttatga ttcaaagcgc tactatatcc atggatatga aaatgcagtg
     5701 actgtcaaac tttccagtgc aaaccgagtt cagttgaatg cagaggcaaa cgaagataca
     5761 cggatgttcc gcgtcacagc agatatcact aagctgggcg aaggaacaca tgaagttccg
     5821 ctaaaaattc aaaatttgag cagtgctgtc acagcaaaga tcgaaccatc gactattaca
     5881 gtaacggttg agaaaaaggt aataaaagat ttcgatgtgg aaacgttgat tccttcaacg
     5941 ttcacaccat ctggatatga acttgatgat acttccgtta gtcctaaaaa agtctctatc
     6001 actacaggtg acaaaacatt ggcagaaatc aaacgagtag tagctgatgt ggacgcttcg
     6061 atggtcacag atgatgggat taatagtgag gtcccaatac aggcgctgaa tgcagcaggg
     6121 gaagtattaa gtatcgtctc tgatccagtg caagtaacag tgagagctga ttcgatcaag
     6181 ccaagcaagt cggtacgact ttatgggata cagcaaggaa caccagctgc cggggtagaa
     6241 agctatgatt tcagtttttc aaaactggaa gcagaagtaa gcggctctag cgatttgctt
     6301 gcatcgatcg gtgacagtat tgctgtaccg atcaatgtct caggtatcaa tcatcgaaca
     6361 actcgtaaaa ttgaaatacc agtagaagag gggttaagta tcaagcccga atcagttagt
     6421 gtggagatta cacccgtgtt gcaaacgtca agcagtagct ctgcttcgac gagcagtcgg
     6481 acacaagaaa atacaagtac gagtagaagt atttcatctg ctgattcagg aacggaacca
     6541 tcgacaacaa ccatttcttc cagttcggaa tctgggacaa gcagctcgac cacaagtaca
     6601 gaagaaacat cagaaacaag cgaatcaaca caaatgagca gaacaaattc ctcagaaaat
     6661 taagttaaag gagtaatttt acaactatgg gtaaatattt tggaacagac ggcgtacgcg
     6721 gtgaagccaa caaggaactt acgccagaac ttgcatttaa gctgggacgg tgcggaggtt
     6781 atgtcctaag ccagcatgaa gaagaaggaa aacgcccacg cgtgttagta gggcgggata
     6841 caagaatctc aggtcaaatg ttagaagaag cgctgatttc aggactttta tcggtgggta
     6901 tcgaagtgtt ccagctaggt gtgatctcta caccaggtgt tgcttatctg acacgtttgc
     6961 aaaaagcaag cgcaggggtc atgatttctg cttctcataa tccagcacaa gataatggaa
     7021 tcaaattttt cggtgcagat ggttttaaac tagtagatga ccaagagcta gaaattgaag
     7081 cattattaga tgctgaagtg gatgaattgc ctcgtccatc agctgaagga ttgggaactg
     7141 tagaagaatt tccagaaggc ttattgaaat attctcaatt ccttgtacaa acgatcaacg
     7201 gtgacctttc cggtttgacc atttgtgtcg atgcagcaaa cggagcaact tcaacaagtg
     7261 ttaatcgttt gtttgccgat cttgaaacag atttttatac aatgggcaca aatccagacg
     7321 ggttaaatat caatgacggc gtaggctcta cccatccaga acgtttagct gagatggtcg
     7381 ttgaaaaagg agcagatgct ggactagctt tcgatggcga tggcgatcgt attattgcgg
     7441 tggatgaact tggaaacatt gtcgacggtg ataaaatcat gtttatctgt gctaaatact
     7501 tggcagaaaa aaatcgattg aaaaaagata cgatcgttac gactgtcatg agtaacttag
     7561 gattccacaa agcggtggaa ggaatcggtt tgaaagatgt cgttacacaa gttggtgatc
     7621 gttacgttgt agaagaaatg cgcaaaaacg attataactt tggcggcgag cagtctgggc
     7681 acatgatttt cttagatttt aacacaactg gagatgggat gctttcagga attcagttac
     7741 tgaatatcat gaaacagaca ggtaagaaat tatcagagct tgcagcagag gtaacaatct
     7801 atccgcaaaa attagtgaat atccgcgtaa gtgataaaaa cggtgcgatg gatgttccgg
     7861 caatcaaaaa agtgattgaa gaggcagaag ctgaaatggg atcagaagga agaatcctag
     7921 ttcgtccttc cggtacagaa ccattacttc gagtgatggc agaagcgcct actgatgaga
     7981 aagttaatta ctatgtcgat aaaatcgcgg cagtagtcaa agaagaaatc ggactggatt
     8041 aagagatatt ttcataaaaa ataaaagaac aaggaccagt gtcagatgtc tgacactggt
     8101 ccttgttctt ttattattct actgtcactg atttcgctaa attacgtggt ttgtcaacgt
     8161 cgaatccccg taatagcgtt gcgtaatatg caagtaattg agtcggtacg actgtggtta
     8221 gagtagttag ataagggtgg acagttggta gcaccagctg gtcactttcc ttttctaatc
     8281 cctctgagac aataactagt gttttagcgc cacggctttc gacttctttc aggtttcctc
     8341 gagtatgcgc accagtcact tcttcactga tgattccgat cactggtgtt ccttcttcga
     8401 tcaaagcaat cgttccatgt ttcaattcgc cagcggcaaa accttctgcc tggatataac
     8461 tgatttcttt caatttcaaa gcagcttcaa gagaaacgtt gtagtcagca ccacgtccaa
     8521 tgtaaaaggc atttcttgta tcgctgagat atacttctgc caactcttct aaataatctt
     8581 tttgggcgat gaccgattcc atcgcattag caactaaact caattcatga gtgatatcga
     8641 atacaagaga agtttcaaat tcttttttat caccgatagc tttggctaat aaagttagta
     8701 cagctatttg tgcagtataa gctttcgttg atgctactgc gatttcgggt ccagcatgca
     8761 atagcagtgt aaaggatgct tcccgcgaaa gagtagaacc agcaacattt gtaatcgtaa
     8821 gtgaagggta gccgagacgg ttgattttta ccagcacttg gcggctgtct gctgtttcgc
     8881 cactttgact caagaaaata aagaatggtt tttttgaaag aagaggtgtg ttatagccaa
     8941 attcactggc taaatgaacc tcaacaggaa tctgtgtcaa tttttccaaa gtctgtttac
     9001 ctgctaaccc cgcatggtaa ctagttccgc aagcaacgat atagattcga tcgctatcag
     9061 acaatgtatc gaccagcttt tgatcaagag agacacgatt gtcttctgtt acgtattttt
     9121 gagcgatacg acgcatgata ataggctgtt cgtcgatttc tttcagcata taaaaaggat
     9181 aggtaccttt ctcgatatct gaagcatcta cttgtgcttc gaatgaagaa cgttctacga
     9241 tgtcacctgc aaaattttga ataataactt tttctgatgt gacggtaacc atctcttcat
     9301 cttcaatctc aacgaattcc ttcgtatgag caagcatagc cattgcatcg ctggcaatca
     9361 cattgaatcc atcgccgagt ccgattaata gtggactttt atttttagcg acgtaaataa
     9421 catcaggtgc tgtccgatca atcaaagcga aagcgtaaga acctttgatg acccgcaaag
     9481 cttttaaaaa cgcctcttgc gttgtagtat ccttcgcgaa agtttcaatt agatgagcga
     9541 taatctcagt atcggtctca ccaataaagt ggtcatttgc taaataagca attttaagtt
     9601 cttcaaagtt ttcgattacg ccgttgtgaa cgagcacgaa gcgtccactt tgggaagtat
     9661 gtggatgggc gttttcttca gaaggttgcc cgtgagttgc ccatcgtgta tgaccaatac
     9721 ctatatttcc tgtagtatca atatttattt tgtttttcaa attttgaatt ctgccttgtg
     9781 ctttgaccag ataatcagtt gtgccatctg agacaaagat accagctgaa tcataaccac
     9841 ggtattctaa tttttctaga ccgtcaatca atcctggtgt tacatttttt aatccaatca
     9901 ttccaacaat tccgcacata aaaaacatcc tactttctat aatgaattcg tatgaatgct
     9961 tccagctgca ggaaaatcct tttgttttat ctttgcagac attttttgtg gattttctgt
    10021 agagtgcagt ccctagggcc atccgccgaa tctttcgata aaccctctcc tcgtcatctg
    10081 tttacacaga cctggcgcta tcctaaaagg tataactatc ctcctttttg ttttttatct
    10141 ggaaacattt aaaatcataa ccttttgcga atgttcttgt caacaaaaaa agaataaaaa
    10201 ataaattggt atatatcaaa ttctttgtca aatgtccttt ttatgtcaaa aaataatggg
    10261 tttttatcta tcatcttttt caggagttcg gtacaataga aagtaatcgg atagaggagg
    10321 agtaaaaatg gattcaaaaa ttgagatcat gacactggga atgttaaaaa aacaattgtc
    10381 caaattcgaa gcatcagctg gtgtatcaga tgatacgaaa atatttttag atactggctg
    10441 ggatagcatc caagaaatcg cacctgatgc cttggaagtt gctcaagctc gtgagtttac
    10501 ggtagaagat gaattaacaa aagagtcgtt ttccgggtat gcgcgggaag aaaaagcaga
    10561 gcggttcgac acttctgagc agtcagaaac tgtgatcgtc ataaaaaact tgtattagaa
    10621 aggagaaaga atgaataaaa gacgttgggc agcagttctg attgctgcag gattatttgt
    10681 ggtttcgtta gtttcagcta gtttgactaa gcctcaggaa aacgatcaaa ttgagggatt
    10741 gaacagttgg ttatatgggg atgaagaact agctccaaat gtgttggaag agggagacag
    10801 tacgcaaaaa atcgtaaaat tatcagtaga tggaacgatt gctgcgggag ggtcttcagg
    10861 tttatttgct tctgaaggtt acaatcataa aaattttatg gaacaactga aggcgattga
    10921 agaagatcca acagtcaaag gtatcttttt agaagtaaat tctcctggtg gcggtgtcta
    10981 cgaaagtgcg gaaatcgcta agaaattgga tacgattcgt aaagaacatg atatcccaat
    11041 gtatgtcagc atgaaaaaca tggcagctag cggtggctac tatatttctg ctcaagcaga
    11101 taaaatcttt gcaacagaag aaacagtgac cggctcaatt ggtgtgatca tgtcaggatt
    11161 gaattattca ggacttctag aaaaactggg tgtggaagat acaacagtta aaagcggagc
    11221 gttgaaagat atgggttcag gaacgcgacc agagacaaaa gaagaagaag cggtgcttca
    11281 agcatatatc gacagcgcat atagtcgttt tgtagcaatc gtcagcgagg gacggaataa
    11341 atctgaagaa gaagtcaaaa aaattgctga tggaaggatt tatgatggtg tccaagcaaa
    11401 agaagtaggt ttagtcgatg aattgggatt tcctgaagat gctttaaaag ctatacgaaa
    11461 ggaacaacag cttgaagatg cggaactagt agaatattct tccacgccat ctgggtttgc
    11521 tagtacatgg ttaggaagta aactagcaga attccaggga ttgaagcctt ctgagactag
    11581 tcagatctta tccgttcttg agaatctagg gacagcggaa tcaccaaaag caatgtatta
    11641 ttacggaggt gagtaagcat gtcagagctt tcaaatgaaa ataaggaaca agaaaaagat
    11701 aagcaacagc agcctgctgt aggacctgac tccaactttt cccaaaaggt actgagctct
    11761 tttgaaagag agcctttgac gcccgaagaa atcaaaaaga aacaacgtaa atggaaaaag
    11821 tatacggaca caagaaatga accgccaaaa gcaaatgatt ttcctaagta tttctatgca
    11881 ggttttttta tccgaatgtt tgccttcttg gtcgatcttt tgtgtatcgg agctattacg
    11941 cgtttaacac ttggtattgc caaaaatcta ggttggctta atttttcaga ttcttatctg
    12001 agtctctacg gattcacagc cttacttatt tatctagcgt attttatttt attgacgaaa
    12061 ttcaatcatg gccaaacgat tgggaaaatg atctttggta ttcgagtcat cagctttgat
    12121 gaagaagaac ttagctggtc aaccgtttta gttagagaag gtgcttgccg gttcattcta
    12181 aaatttcctt tgctgatgtt agggtatctt ccagcagtct ttaatcagaa aaagcaacat
    12241 ctgggcgatt ttttcactaa tacaagtgtt gttacgatca atctgatcaa agcgttcaac
    12301 caggaagtca acagctgatg agcaataaaa agcagtaaca gttgaaagca ccttctaaaa
    12361 gtcagatcta aaatctagct tttagagggt gcttcagtta tttttaattg tcatgctttt
    12421 tttgttttgc tactattgaa catgagctga atatgagcaa gaggaggaca ctgcgtgaag
    12481 ccgattgatt ttccagaaaa atatgagaat ttaatgcgag tggcacaaca agcactagca
    12541 aaccagcaat atcagcaggc aaaagaatta tttcaacgag cttacgagtt gaaagaaagc
    12601 tttgaggcta atagcctgct tgttttttgt ctttacgagt tggacgagaa aaaagaagca
    12661 ttgaagcaag cactcttgca tgaaaaacag tatcttcaaa atgaggagtt tgctgaattt
    12721 tattttgact tgttaatcag tacacaagat tttttatatg ctagaaaatt gatagcaagt
    12781 acagattttt atgaagcttt cgagcagcgg gtcattgaaa aaatccaatt tgcagaagaa
    12841 ttatccggac aaatggagag gcagaagatc aaggcactcc acaataaatc ggaggaactt
    12901 ccttcgttgg aacctgcacg tcagcttgcg tctattgatg agatcgaaca gcttccttat
    12961 catgagttta tccaaacagc gagtaaactt attgtatcgc cagaggtcca tattttagca
    13021 agggcaaaat tactggagac acttaggcaa ttgaacgaat gccatcctgt tttctatttg
    13081 acgattgaag aaaagttagt caaagtcata ccaaaagatc tgccaaagcc gcagcagcag
    13141 gcaagttacc agcaattatg tgtattttca gatcgttacg ggaatgaaga tgcgctgctt
    13201 agttccgtgt taaaagaaga attcacgttg caatctgcaa ttgtttatcc tgtctatgat
    13261 acatacatcg aagacccgaa gcgctggttt caactgactg tagaggcgtg tacaggaaaa
    13321 acgatgtatg aagggacaga agatgaaaaa caagattttt taaaaaaaag agaaaaaatt
    13381 ttgcagcaaa tgatcttctt ccattagatt ttatcagtga aaaagataaa aatgttatga
    13441 aaaacaagga ttctatccat tcaatatgtt tacaatttga aagggttagt gtactattta
    13501 aaggtatgca tgaaacgttt ttgatgtata aaatgaattt ctaaactcgg agggaattaa
    13561 atgactgcaa catttgaaaa aaaaggcacg aatgatggtg ttttaaaatt tacgattgcg
    13621 caagacgaaa tccaaaaagg attaacaact gcgtttaata aagtgaaaaa aagcttgaac
    13681 gtacctggat tccgtaaagg gaaagtttct cgtcaagtat tcaaccgtat gtacggtgaa
    13741 gaagctttat atgaagatgc attgaatgct gtattaccag aagcttatga agctgctgtc
    13801 aaagaagctg gcatcgatcc tgtttctcaa ccaaaaatcg atgttgaaag catggaaaaa
    13861 ggtcaagact gggtaatcac tgctgaagta acagtgaaac cagaagtaaa attaggacaa
    13921 tataaagatc tagttgttga aaaacaagac cgtgaagtaa cagatgctga cgtggatgca
    13981 cgtatcaaac gtgaacaaga agcacaagct gaattagtta tcaaagaaga tgctgcagct
    14041 gaaaatggcg atacagtagt aatcgacttc gaaggctttg tagatggcga agcgtttgat
    14101 ggcggaaaag gcgaaaatta ctcacttgaa ttaggttcag gctcattcat tccaggtttt
    14161 gaagatcaat tagttggtca taaatcaggt gaaagtctag atgtaaatgt aactttccca
    14221 gaagattatc aagcagaaga tcttgctgga aaagaagcag tcttcaaagt gacaatccat
    14281 gaagtaaaag caaaagaagt gcctgaatta gatgacgaat tcgcaaaaga tgttgatgac
    14341 gaagtatcaa cattagacga attgaaagaa aaatatcgca aagaattagt agaaacaaaa
    14401 gaaaaagcag cggacgatgc aaaagatgaa gcagcgatcc gtatggctgt tgaaaacgca
    14461 gaaatcgttg aattgccaca cgtgatggtt catgatgaag tacatcgttc aatggacgaa
    14521 ttcttgaaca acatgcaacg tcaaggtatc tctcctgaaa tgtactatca attaactggt
    14581 tctacagaag aagacttgca taaacaattc gaaggtgaag cagaaatgcg cacaagaaca
    14641 aatcttgtga tcgaagcaat tgcagctgct gaaaatcttc aagcaacaga agaagaaatc
    14701 aatgcagaaa tccaagaatt atcagaaaca tacaacatgc caatcgaaca aatcaaacgc
    14761 gtgttgacag aagatatgct ggaacatgat gtgacaatga aaaaagcaat cgaattgatc
    14821 acaggtacag cgacagaagc gaaagaatag aaatcaagta ataaatcatt tctgaagaag
    14881 ctacttctag aaaattgttt attcgaaata agggactgtg acacgatttt gtcacagttc
    14941 cttatttttt gttaagagta aaaaatgcaa aggtataggt cagttgatcc tttgttaaac
    15001 actttttctt tttactaaac tatgttatga tagtaaaaga ctgtaaagtc tttgatagtt
    15061 tcttgcatac tttttgcaag ttaaggggtg aagaccatgt atgacaatcc aagtagcaat
    15121 gagaccgtac gctgttcttt ttgcgggaaa acccaagaag aagtgaaaaa gatcgtagct
    15181 ggtccagggg tctacatctg taatgaatgt atcgacctat gtaaagaaat cattgatgaa
    15241 gaattttatg aagaagctgt acgcgaattc actgaagtcc ctaaaccact agaaattctt
    15301 gaagtattga ataactacgt gattggacaa gatcgcgcaa aacgctcgtt agctgttgcc
    15361 gtatacaatc actataaacg tgtcaatcag caagcacaag aaaacacaga tgatgttgaa
    15421 ctgcaaaaaa gcaatatctg cttgatcgga cctacagggt ctggtaaaac atttttagca
    15481 caaacattag caaaaacatt gaatgtacct tttgcaatcg cagatgcgac aagtttgaca
    15541 gaagctggtt atgtggggga agatgtagaa aacattttgc ttaaattgct tcaatctgct
    15601 gattacaatg tggaacttgc agaaaaaggg atcatctata ttgatgagat cgataaaatt
    15661 gcgcgtaaaa gcgaaaatgt atctattact cgtgacgttt ccggcgaagg tgtccaacaa
    15721 gccttgctga aaattctaga aggaactgtt gcaagtgtgc cacctcaggg cggacgtaaa
    15781 catccacatc aagaatttat tcagattgat acgacgaaca tattgttcat cgtaggtggt
    15841 gcatttgatg gcatcgaaac gattgtgaag aatcgtatgg gcgaaaagac gattggattt
    15901 ggtacacaaa accaaaaact ttctgaagac gaaagtgtca tgcagcatat cattcctgaa
    15961 gatttactaa aattcggatt gatccctgag tttatcggac gattacctgt tatggcagct
    16021 ttagagaaat tgacaacaga tgatctggta cggatcttga ctgagccgaa aaatgcgcta
    16081 gtcaaacaat atcaaaaact tctttctcta gataataccg aacttgaatt cgaacctgaa
    16141 gcgttgagag ccattgcgaa aaaagcaatc gaacgcaata ccggtgctcg aggacttcgt
    16201 tccatcattg aagaaatcat gatggatgtg atgtttgata tcccttcaaa tgaaaacatc
    16261 gaaaaagtga ttatcacaaa agaagcagct gaattgtctg gtgagccaac agtaatctat
    16321 aaagattcag agaaaaaagc gggttgatga aaagaggctg ccgtaaaagt cattttggct
    16381 tttgcgacag ccgcttgctt tattcatcac cttgttaagg agagagacca tgaaagtcca
    16441 tcaagctgaa attatcataa gtgcagtggc accaaaacaa tatccagata cacaattgcc
    16501 agaaatagct ctggcaggac gatcaaatgt agggaaatct tcttttatca acacattgat
    16561 tgatcgaaag aatttagccc gtacttccgg taagccagga aagacccaga cattgaattt
    16621 ttatttgatc gagaacgcat tacattttgt cgatgttccg ggatatgggt atgcaaaagt
    16681 atccaaaact gagcgagcaa aatggggaaa gatgatcgag acatatttaa cgactagaga
    16741 ccagctacgt gcggtcgttt ctttagtaga tttgcgtcat aagccttctc aagaagatat
    16801 ccagatgtat caatttttga agtattatga tatccctgta attgttgttg ccactaaggc
    16861 agataagatc cctcgtggta aatggaacaa gcatgagagc gaaatcaaaa aagcgttgga
    16921 ttttgatcgc aatgacacgt ttattctctt ttcttccgtt acaaaacagg gaaaagaaga
    16981 agcgtgggca gcaatcgaac aagcaattga atcataaaaa gaataaaaaa gacttaccag
    17041 tcaaactctt tcataaaagt ttggctggta agtctttttt tgagcttaac ttacttttgt
    17101 ttcttgtctt ttttcttttc attttgtggc tgtggtttta gaaatcgatc gatctcttct
    17161 tttggtgcaa ctgttttttc cggatcgaca gcaaatgatt cgaaaagttt atctaaatta
    17221 tctgtacgtt caaatttcaa ccccattgta aacacctctt tgtttctttt tacttagtct
    17281 atcatgtaat cgtccaaatt ggtttctaag acataaagtt ttagtttttc tttatcattg
    17341 gttataatta aattataaaa agatttgaaa gaggtgagaa aatgattggc gaagataaat
    17401 tattgatgac tatccagctc acagacttaa atgaagaaga aaaaaacagg aaataagcga
    17461 ttgggctatt ttgacacgtc tgttgatcca gccaacattt atccgctctt tcacacaaca
    17521 agcggatccg tatgatcttc gaaagcttca agaaaaaata atgctagcat cggtaagaga
    17581 cgaaagttgg ttagttgtag gaaatgacga gaacgaatgt agttttcgct tggaagataa
    17641 tcaattattg ataaaaaatg tattgtctat ctctgttttc aaagaaaagc aatttttgat
    17701 tcgagacttc attcagaaaa aaatgattgc tcatggggtg ttcgcctata tgcgagctta
    17761 tagcgagttt atctatcaca atacaaaaca gatctctcaa cggctattgt ttgagaaaaa
    17821 ggatgaaatc gaacatttac ctaaaatgaa acaacaaaac ggggaagtag tagtcgattg
    17881 caaccaattt ccaggatacg atctttttta ccaaggttta tgtttcactt cgtgttggga
    17941 aatgtattat agtcgctatt atcatcaaat catccctaaa ccgatttttc tcgacatcca
    18001 acaagtagaa aaagtgaagg aattagaaaa cgaagtcata tgtatccagc tttatcgaga
    18061 cccctttaac tggcaaaagc caaacaatca aatgtttcaa tcctatttta gggaccagct
    18121 tgggtttgat catcttgctt gggacaatgg tgtcggttta ttgaaaccgc cattcgttga
    18181 atacatttac actgaccata cgatccaatc tgtccagtat cagaatgcac aaatgcaacc
    18241 tgttcctaaa aagaatgctt cattttttgt aacaaaaagt tatgatatcc aacaaggaga
    18301 ttataaagaa aggcgtgttc gcggaacact aaatgctcag gcgtattttc cttgggttga
    18361 cgagaatcga tcacgcatga tgtgctataa agttatcgat ccgactgttg ctttggataa
    18421 tggaatcgaa gcttattgtt attatattag ggaatatcta gaagtagagg taacagatga
    18481 aaaatatcaa gagtatcttt tatccttaag aatctatgtt ccttcagaat ccctgtcaga
    18541 attaccgtta aaggaaatca aacatagact ctctgatgtt accttcaaac gaataaagaa
    18601 aaagcggcga agtatccaat tcgatgtcaa gaaaggtgag cagcatctgc gtgtgcaatt
    18661 tttagattac cgagaattag aacaattgat tactttacaa aaaatatgag gtgaagaaaa
    18721 tggataaaca actctattat cgaataatgg ttccggcatc attattatcc gtctgtctct
    18781 tttttgttcc taattttcct attcgatcag tcatcggttt tttggtcatg tttggcggga
    18841 tgggacttta ttattatctt cttcataagg aaagaaaacc tgcttctgtt tatatgaaaa
    18901 aaacaagaag cgaaaataaa aaacgataaa acggcttgat gatagacaaa aaaataa
//
LOCUS       DACJGB010000061        18718 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.061, whole genome shotgun sequence.
ACCESSION   DACJGB010000061 DACJGB010000000
VERSION     DACJGB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.061"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(510..1076)
                     /locus_tag="IYI34_002167"
     CDS             complement(510..1076)
                     /locus_tag="IYI34_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAR0560013.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="IYI34_002168"
     CDS             complement(1076..2032)
                     /locus_tag="IYI34_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAR0560014.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="IYI34_002169"
     CDS             complement(2032..2688)
                     /locus_tag="IYI34_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAR0560015.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="IYI34_002170"
     CDS             3058..5076
                     /locus_tag="IYI34_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR0560016.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5503..7917
                     /locus_tag="IYI34_002171"
     CDS             5503..7917
                     /locus_tag="IYI34_002171"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAR0560017.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="IYI34_002172"
     CDS             8265..9818
                     /locus_tag="IYI34_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAR0560018.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="IYI34_002173"
     CDS             complement(10199..11983)
                     /locus_tag="IYI34_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0560019.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="IYI34_002174"
     CDS             complement(11998..13719)
                     /locus_tag="IYI34_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0560020.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="IYI34_002175"
     CDS             13819..14580
                     /locus_tag="IYI34_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR0560021.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15547..15815
                     /locus_tag="IYI34_002176"
                     /pseudo
     CDS             15547..15815
                     /locus_tag="IYI34_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16487..>18718
                     /locus_tag="IYI34_002177"
     CDS             16487..>18718
                     /locus_tag="IYI34_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560022.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6151 a         3325 c         3763 g         5479 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtagcc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaactcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 acattattac aatatgttaa taaggcaaaa ataaatagat ggattgaata aacatatttt
    15001 ttaatcaaat agtccctact gtgactttcc tcagctatcg gtgatactac cgatagcatt
    15061 tttttgctta taaaataatt ataagtattt agagattttt cttctaacat tcttcaaaca
    15121 agaaaaatca caaaggttat tgttattttt atatattctt tttaagaatg attgtttttt
    15181 tatagaaatt ttaaaaaata aaacacgtta ttgatggata gttagtttgt tatttggtaa
    15241 tattatagaa gttgactggt agtgatttat ttttcgtcgt tttataaaaa tgttattttt
    15301 tggaaaaatt cacaaatatg taacgaatgt ttttctggaa tttgctgtgt taaaaagcag
    15361 tatgactaat ataaatacta ttgtcattta tgcaattttt tatggatagt aattttagag
    15421 gggaatcaat gaaggaagaa tagaagggaa aaatagttgc aaatacgcca gttagcaaaa
    15481 atagcaatac atcttcaagc tttaaaattc gagagaatca tcttggtagt ggaatcaact
    15541 acaacaaaag gattaaacca attaatagat agaattacca atggaaaaat agaaaaggta
    15601 gttattctat acaaaagttg tttaattcgc ttcggttatg aacttagtga aaccctttgc
    15661 aatatatggt acagaatata gaaattattg acattacaga aaatgatgaa gatttaatac
    15721 agattgtcac tatttgtaga taccttttac aaggaaaccg agccaataaa gctaagaaga
    15781 tgattaagga gttgatggaa gacgatacaa gttaagaaag tgaaactacg cccaaataaa
    15841 gagcaaaaag agtaattgga cgtggaaaat gcaagaaatg aaccatcgat tcggtggaca
    15901 gtttattagt aatgacggtt tgagaacatc catgacgaga atgaaggaac atcctaaatg
    15961 cgtttagttg aaccaagtgc caaataatat ggccaaataa gcgatcagtt atcgatgaat
    16021 cccaagtatt ctaaactata tattcatttt gatgctaagc attgatatat ttttttggga
    16081 ttgatgaagg ttaaccaata cagggactca ctgatgaaat tctagagtct gaccttggta
    16141 tcaacgattt agtgattgtt tctaatggaa aagtataaaa gtatataagt acattaacaa
    16201 aataactatt agggttacgc tccagcttgt gcaaggcact ggcaggagga atcgtgaaat
    16261 cataattatg gttaagccac acggacaact aaaagtcata caaagctatc gaatcaatca
    16321 acagaagaca atcggcaatc attagctttg tttacagctt acattgcaac gcttgagttg
    16381 aaccgaaaat acgagggagg taacgcagcc attttgagtt ggggaatctt tttagaaaat
    16441 tatcgggtac cttgttgttt agtcaaaatt ataaggggga gtaatagtga ggaacaaatt
    16501 tcataaactg ttaagtatgt ttctagttat gcttattgtg atcaatcaat ttgggaacat
    16561 aacggttcta gctgagtctt tagcagaaca gccagagcgt tcgctgacga tcgaagttga
    16621 tcaagaggag gctgctttgg gtgagcagtt ccagttgcga gtcagaggaa caccagacct
    16681 gctaaatgca ttggtcattg ataagagtga ggatttgatt gaaggggaag tgaagcaact
    16741 caatgattac gaacgtctga ttacttattc ttcccagcat tcaggtaaat acgaattgac
    16801 cgcaagaagc ggtgatattc aatcaaatac ggtctcaata gaaatcagtg taccagaaga
    16861 aagcacaagt gagtctttta atgtaccaga agaaagcaca agtgaatctt ttaatatacc
    16921 agaagaaagc ttaagtgaat cttttaatga agccagatca tctgagccat ctataacgca
    16981 ggaatctcag attgaaagac aagaggagct cttgaattat gacatcggca tctctgctga
    17041 gcgccccact ttgccgagca ggcatacaca aacgttaaag ggatacattc aaggaggcgc
    17101 tggcgatatt cttgaagaat atggggagat ggtcgtacga gttcctagag catcgattgg
    17161 cttaattgag ttcattagag tggaggtcgc tgatgattca cctttaaagc cactggcatt
    17221 ttaccaagaa ggcacagacg ttgtttttcc atttaccttt gattatgaag gattaaatga
    17281 agccgctcag tcgtatacat tcagtatcac gtttgaagtt gctttctttc aatcaggcgg
    17341 tactgagcca aataatgact ttcaagtgag tcttacttat gctgagcgag cagcaaacac
    17401 ggaattttca attacgccta ttccttcatc aagacccatt ttttcgaaat tatactatgg
    17461 ctctagagaa atcgacagcc aaggaaatct tgggaatctg ctgattgacg ggcaagacgt
    17521 gaccaacagt aataaattta ttttgcaggt aaattatgaa ggatcacgaa aagacggtgt
    17581 agtggttacc gatgtactac cgcaaggaac aactttggca ccgaatccga ctacatggag
    17641 ttccactgga aatagagaac ctatttcagg tggggttcgg atcgcagaag taacgaatcc
    17701 aggtgaagca aacgttagaa ctcattatgt ttccgaggaa tttgtggaca aagtatcttt
    17761 taataaggaa acgaatacac ttgttgtgga ctttggtaat ctagcagagg gtcaatcgta
    17821 ttttattgaa tatgctcttg aagtgaccga tgtcgacttg ttcaatgaag gaagacaaga
    17881 ggatgggttt tctcggaatc gagcgagtat gacctacaat aatcaggtaa tgagccgaga
    17941 ctaccgcttg agaatccctg aaagtaatct tccggcaatc tctttaagaa aaagtgtgga
    18001 caaaaatgtt atcaatctca atgaaaatga actaacgtat actctgcatg tctcgatgtt
    18061 ttctggaaca agaaatacgc ttgttattga agatgttttg gaagaaggca tggaatttgt
    18121 gtcatttcta tcagatattc cctctttcat tgatgccgaa gtcaataatg gaatagttcg
    18181 tttcagtgcg aataatacga ttggtggtcc gacaagtttt accctctctt ttaaggtcaa
    18241 tgtagaggcg tatgcggtcg gcgatcaaat tttaaacttt gcaacgcttg tgaatagtga
    18301 caatcgattg aatacgagta ctgtaagaac aaggaaaatc gatgggcgta ttaaagtaat
    18361 taaaactgat gagaatggcc aaaggctggc aggagcaaaa tttgaaattt tatcaagcga
    18421 ttcacaagtg atacaagaag gtataacggc agaaaacgga gaatttttaa gtgaaccgct
    18481 tcctgtaggt agctatcaag tccgagaaac agaagcaccg gaagggtatc ttttagatgc
    18541 gacggtacat gatgttttta taaatgaata ctccatagaa gaagtgacgt taaccattga
    18601 aaacatcctt gatacgggtg gtgttgaact aattaaacat gctgaaggca gtggtgaagt
    18661 ccttcaagga gctgtttttg agttacagaa ccgagaagga gaaacgctac aaaccggt
//
LOCUS       DACJGB010000062        18022 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.062, whole genome shotgun sequence.
ACCESSION   DACJGB010000062 DACJGB010000000
VERSION     DACJGB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.062"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            214..498
                     /locus_tag="IYI34_002178"
     CDS             214..498
                     /locus_tag="IYI34_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAR0560023.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="IYI34_002179"
     CDS             complement(815..1198)
                     /locus_tag="IYI34_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAR0560024.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="IYI34_002180"
     CDS             complement(1200..1784)
                     /locus_tag="IYI34_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAR0560025.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IYI34_002181"
     CDS             complement(1831..3306)
                     /locus_tag="IYI34_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAR0560026.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IYI34_002182"
     CDS             3487..3798
                     /locus_tag="IYI34_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560027.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IYI34_002183"
     CDS             complement(3913..6249)
                     /locus_tag="IYI34_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0560028.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IYI34_002184"
     CDS             6391..7059
                     /locus_tag="IYI34_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560029.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IYI34_002185"
     CDS             7062..8105
                     /locus_tag="IYI34_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0560030.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IYI34_002186"
     CDS             complement(8342..9223)
                     /locus_tag="IYI34_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR0560031.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IYI34_002187"
     CDS             9382..10110
                     /locus_tag="IYI34_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAR0560032.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IYI34_002188"
     CDS             10266..10583
                     /locus_tag="IYI34_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR0560033.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IYI34_002189"
     CDS             10576..11184
                     /locus_tag="IYI34_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAR0560034.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IYI34_002190"
     CDS             11200..12030
                     /locus_tag="IYI34_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAR0560035.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12656
                     /locus_tag="IYI34_002191"
     CDS             12168..12656
                     /locus_tag="IYI34_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAR0560036.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="IYI34_002192"
     CDS             12707..13579
                     /locus_tag="IYI34_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAR0560037.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="IYI34_002193"
     CDS             13755..14117
                     /locus_tag="IYI34_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR0560038.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="IYI34_002194"
     CDS             14126..14878
                     /locus_tag="IYI34_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0560039.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="IYI34_002195"
     CDS             14892..16694
                     /locus_tag="IYI34_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0560040.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="IYI34_002196"
     CDS             16971..18011
                     /locus_tag="IYI34_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAR0560041.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5657 a         3126 c         3723 g         5516 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aa
//
LOCUS       DACJGB010000063        17712 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.063, whole genome shotgun sequence.
ACCESSION   DACJGB010000063 DACJGB010000000
VERSION     DACJGB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.063"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            138..995
                     /locus_tag="IYI34_002197"
     CDS             138..995
                     /locus_tag="IYI34_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAR0560042.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1110..2030
                     /gene="pstC"
                     /locus_tag="IYI34_002198"
     CDS             1110..2030
                     /gene="pstC"
                     /locus_tag="IYI34_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAR0560043.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2030..2914
                     /gene="pstA"
                     /locus_tag="IYI34_002199"
     CDS             2030..2914
                     /gene="pstA"
                     /locus_tag="IYI34_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAR0560044.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2929..3735
                     /gene="pstB"
                     /locus_tag="IYI34_002200"
     CDS             2929..3735
                     /gene="pstB"
                     /locus_tag="IYI34_002200"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAR0560045.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3751..4509
                     /locus_tag="IYI34_002201"
     CDS             3751..4509
                     /locus_tag="IYI34_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAR0560046.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4522..5199
                     /gene="phoU"
                     /locus_tag="IYI34_002202"
     CDS             4522..5199
                     /gene="phoU"
                     /locus_tag="IYI34_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAR0560047.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5437..5736
                     /locus_tag="IYI34_002203"
     CDS             5437..5736
                     /locus_tag="IYI34_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560048.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5762..6469
                     /locus_tag="IYI34_002204"
     CDS             5762..6469
                     /locus_tag="IYI34_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAR0560049.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6466..7233
                     /locus_tag="IYI34_002205"
     CDS             6466..7233
                     /locus_tag="IYI34_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAR0560050.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7333..8901
                     /gene="liaX"
                     /locus_tag="IYI34_002206"
     CDS             7333..8901
                     /gene="liaX"
                     /locus_tag="IYI34_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAR0560051.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9201..9518
                     /locus_tag="IYI34_002207"
     CDS             9201..9518
                     /locus_tag="IYI34_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAR0560052.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            9518..9868
                     /locus_tag="IYI34_002208"
     CDS             9518..9868
                     /locus_tag="IYI34_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAR0560053.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10035..10973
                     /locus_tag="IYI34_002209"
     CDS             10035..10973
                     /locus_tag="IYI34_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAR0560054.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10988..11818
                     /locus_tag="IYI34_002210"
     CDS             10988..11818
                     /locus_tag="IYI34_002210"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAR0560055.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11862..12887
                     /locus_tag="IYI34_002211"
     CDS             11862..12887
                     /locus_tag="IYI34_002211"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAR0560056.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12902..13840
                     /gene="galU"
                     /locus_tag="IYI34_002212"
     CDS             12902..13840
                     /gene="galU"
                     /locus_tag="IYI34_002212"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAR0560057.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13965..14405
                     /locus_tag="IYI34_002213"
     CDS             13965..14405
                     /locus_tag="IYI34_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAR0560058.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14408..15010
                     /locus_tag="IYI34_002214"
     CDS             14408..15010
                     /locus_tag="IYI34_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAR0560059.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15078..16184)
                     /locus_tag="IYI34_002215"
     CDS             complement(15078..16184)
                     /locus_tag="IYI34_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAR0560060.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16394..17395
                     /gene="ccpA"
                     /locus_tag="IYI34_002216"
     CDS             16394..17395
                     /gene="ccpA"
                     /locus_tag="IYI34_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAR0560061.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
BASE COUNT         5786 a         3069 c         3809 g         5048 t
ORIGIN      
        1 tagcatagaa acttttaatg agatagtttt ttttacaatt atgagaattt attttgttat
       61 ttaatgattt tgattcgttt tcatcaagta atgaaaatga taaaataaac atactttagg
      121 attaaaggag atttctaatg aaaaaactta aattattctt accaataata ggattagcca
      181 ttcttttatc aggctgttca aagtggattg atcgaggaga atccattacg gctgttggct
      241 cttctgcttt acagccttta gtagaaacag ttgctgaaac ttaccagaca gaaaatcctg
      301 gaaaattcat caatgtacaa gggggcggaa gcggaactgg tttgtctcaa gtacagtcag
      361 gagcagtcga catcggaaac tctgatttgt ttgcggaaga aaaatcgggg atcgatgcct
      421 cagcattagt cgatcatcga gtagcagttg ttgggatcac tcctattgtg aacaaggata
      481 ttggcgtaaa agacatcagt atggaaaacc tgaaaaaact atttacaggc gagatcaaaa
      541 actggagtga agtcggtgga aaagatcagc cagtagttat attgaaccgc gcttcaggaa
      601 gcggaacccg tagcacgttt gaaaaatggg tattggacgg agaaacagct gttcaggcac
      661 aagaacaaga ttcaagcgga atggtgcgcc agatcgttgc cgacacaccc ggtgcaatca
      721 gctatgttgc tttttcttat gtttccgatg atgtagatac gttgaatgtt aacggtgtag
      781 aaccgacaga tgaaaatgtg acaacgaaca aatggacgat ttgggcatat gagcatatgt
      841 acacaagaga tcatccaaaa aaactgacaa aagaattttt agactatatc ttatctgatg
      901 aagtacaaga taacatcgtt ggagaactag gttatatccc tgtttctcat atgaaagtcg
      961 aacgtgattg gcaaggaaat atcgttaaag agtaatcttt acacaaaatt tacaaactta
     1021 gctaaacttt tacatgttat taaaaccaaa ttgatgttca atttgtacac tgagtgagta
     1081 tgttaaacct gattaagggg gagtcatctg tggaagatgt gaaaaaagta ttattaacaa
     1141 aatcaaaacg ttcaaaaatg gaacagcgtg gaaaattcat cagtttttta tgtatcgcgc
     1201 tgatcgtgtt ggttgtatta tctatttttt attttgtagc tagtaaagga ttggccacct
     1261 tttttactga taagatcaat gtatttgatt ttctgtttgg tacagattgg aatccaagcc
     1321 aaatcggagc aaatgggaaa ccaatggtag gggcattgcc tatgatcaca ggttcgttta
     1381 ttgtgacctt tttatctgct atcgttgcca cgccatttgc cattggtgca gcagtattca
     1441 tgacggagat ctctcctaaa aacggaacga agatcttaca gccggtcatc gagctgcttg
     1501 taggtattcc ttctgtcgtt tatggtttta tcggtttatc agttatcgtg ccttttgtca
     1561 gaagtatttt tggcgggaca gggtttggta tcttagctgg tacatttgtg ttgttcgtta
     1621 tgatcttgcc gactgtcaca acgatgactg ttgatgcatt aaaggcagtg ccacggcact
     1681 atcgggaagc ttcattagcg cttggtgcca caagatggca aacaatctac aaagtcgtgc
     1741 tacgagcagc cgtgcccggg attttaacag cagtcgtttt tggtatggct cgtgcgtttg
     1801 gtgaagcctt agctatccaa atggtcatcg gtaatgcagc attgatgcca acaggactta
     1861 ctactcctgc ttctacattg acgtccatct taacaatggg aatcggaaat acaatcatgg
     1921 gaacagttga aaataatgtg ttatggtcat tagcgctgat cttgctattg atgtcattat
     1981 tctttaatat cgtgatccgt atgattggga agaaaggaga gctgaaataa tgaacgcaaa
     2041 acgctcagat aaaattgcta caggtgtttt atatatagtt tcggggatca tcgtattgat
     2101 tttagcagct ttactacttt atatcttagt tcggggaata ccgcatattt catggtcctt
     2161 cttgaccgaa ccttccaaag cctaccaaga aggcggtgga atcgggatcc agctatttaa
     2221 ctcgatttat ttgcttttga tcacgatgat catcagtttt cctatttctt taggtgcggg
     2281 tatctatctt tcagaatatg ctggaaaaaa ctggctaaca gatgtgattc gcacgtcgat
     2341 tgaaatcttg agctcgttgc cttctgtcgt tgtcggttta ttcggtttct tggttttcgt
     2401 tattcaattc cagtatggtt tctcgattat ttctggtgca ttggctttga ctgtattcaa
     2461 cttgcctctt ttgactagaa cagttgagga gtctttacaa gcggtccatt acacacaacg
     2521 agaagcagga ttggccttag gtttgtcacg ctgggaaaca gtgataaaag tggtggttcc
     2581 agaagcgaca ccagggattt tgactggtgt gatcctaagt tctggaagaa tttttggtga
     2641 agcagctgca ttgatctata cggctggaca aagtgcacct gcgttagatt ttacgaactg
     2701 gaatccatta agtgtttcaa gcccgatcag cattttccgt caagctgaaa cattagctgt
     2761 ccatatttgg aagatcaata cagaaggcac gatgcctgat ggtgtttctg tttcggctgg
     2821 cgcttctgcc gtgttgatca ttgctgtttt actgttcaac ttcggcgctc gtgcgattgg
     2881 caaacgatta taccgaaaaa tgacttcagc ctaaggagaa gaagcaaaat gaaaaaatat
     2941 aactggaatg aaacaaatgt catcaccatg ccaaaagaga aagaagtagc actatatacg
     3001 gatgatctac atgtctggta tggcgacaat gaagcaatca aaggagtgga tctggaattt
     3061 gaaaaaaata agatcactgc actgattggt ccttccggct gcggaaaatc tacgtatctt
     3121 cgttcgttga atcgaatgaa tgatgggatc gctaatacga aaatcactgg gaaaatcatg
     3181 tatcaaggcg tagatattaa tgcgcctcaa gtggatgttt atgagatgcg caaacggatt
     3241 ggcatggttt ttcaacgtcc taatccgttc agcaagtcga tttatgacaa tattacattt
     3301 gctttgaaac agcacggcga aaaaaacaaa aagaaattag atgaaatcgt tgaaacaagc
     3361 ttgaaacaag ctgccttgtg ggaccaagta aaagacagct tggacaaaag tgccttggct
     3421 ttatctggcg ggcaacagca acgtttatgt attgctcgtg cgatcgcgat gaaacctgat
     3481 atcttgctgc tagacgagcc ggcaagtgct ttggacccta tttcgacagg aacggttgaa
     3541 gaaacacttg taaacttgaa agaagactat acgatcatca tcgtgacgca taacatgcaa
     3601 caagcagctc gtatcagcga ctatactgcc tttttctatc taggaaaagt aatggaatat
     3661 gacaaaacta acaagatatt taccagacca aaaattcagg caaccgaaga ttatgtgtcc
     3721 ggtcattttg gttaagacgg aggcctcaac atgacaaaag aaattatttc atctaaagat
     3781 cttcatctgt attatggaaa gaaagaagca ctaaaaggga tcaatctaag tttcaatcaa
     3841 ggggaaatca ctgcgatgat cggtccttct ggttgcggga aatccactta tctgcgttct
     3901 ttgaaccgga tgaatgacct gattccgaat gtaacgatca ccggaagtgt tttatataaa
     3961 gacaaagaca tctatggtcc aaaaacagat gttgttgaac taagaaaaga aatcggcatg
     4021 gtattccagc agccaaaccc atttcctttt tctatctatg aaaatgtcat ctatggattg
     4081 aaattaaaag gggaaaaaga taaaaaagtc cttgatcaag tagttgaaga aagcctgaag
     4141 gcagcgtctg tttgggatga tgtgaaagac aaattgcata aaagtgcttt atctttatcc
     4201 ggtgggcagc agcagcgtgt atgtatcgca cgtgttttgg cagttaatcc ggaaatcatc
     4261 ttattggatg aaccaacaag tgcgcttgat cctgtttcta ctgggaaaat cgaaagtatg
     4321 ctgttagaat taagagagcg gtatacgatg atcatcgtga cccataatat gtctcaagct
     4381 tcacgtattt cagataagac ggcattcttt ttagatggtc acttgatcga atacaacgat
     4441 acgaagaaga tattcatgaa tccagaaaaa caagagacag aagattatat ttcaggtcga
     4501 tttggataag gaggaaacgg aatgctacgt acacaatttg aagaagaatt gctgaatctc
     4561 cataatcagt tctacgaaat ggggatgatg gtgagcagcg ccgtgcacaa gtcagttcgt
     4621 gcatacatca aacacgataa aatcttagcc caagaagtta tcgaaaacga tataaatatc
     4681 aataacatgg aaacacgctt ggagaagaaa agttttgaaa tgatcgcatt gcaacagcca
     4741 gttacaactg atttgcgtat gatcattacg gtcatgaaag ctagctcgga tttagaacgt
     4801 atggccgatc atgcggtgtc tgttgctaaa tccacgattc gcgtaaaagg gcagacgaga
     4861 ataccggaaa tcgaaaaaga gatctccgat atgtccgatt atgtgaaaaa aatggtggac
     4921 aacgtcctag ttgcttatgt caaaacagac gaaaaagacg cacgtacgat cgccaatatg
     4981 gatcaaagag tcaatgacta tttcaatcga atctataatg caacaatcaa aaatatgcag
     5041 gaaaatcctg agacagtcat cagtggaaca gactacttga atgttgccag ttatctagaa
     5101 cggatcggag actatgtgac aaacatctgt gaatggatcg tctacttggc tacagggaaa
     5161 atcactgagt taaatacgaa ccacaatgag attttctaaa ctgaagctgt gattatgccg
     5221 tttgtttagg aaaagaggtt gtgaaatcag cggttgtgaa aaagctgttc tgcatcaagt
     5281 aataagaaca gtcaaacaaa aaatagctcc tcatattttt cgtttggctg gacttattac
     5341 cgcagatgca agcttttgaa caccgtttat tcgggaaagg gtttgtgaca aaacatgtca
     5401 caaacccttt tcatcatttc atagaaagga taaagaatgc aaaaaaaaat tagcatcatc
     5461 actatatttt ttatttgtgt gcttacttca tgggttattc agcatcaagc tgctttatat
     5521 gaagtacctg tcatcgagat cgaaaaagta cgaataaaag aggatcatca attgattaca
     5581 ggaagactga tgaacacgga tcaaaaagga gaatcgattt atttatcggt tccttatcaa
     5641 aaaaatcaaa tagaaacagc aaatataaaa gcgagacaaa tgtatctagt ccaaatgcat
     5701 ggagatgaat ggcagctagt agcaaaaaaa aaatgatggc tggcttttct tcttcacaag
     5761 tatctccatt ggcgccatgt tgcttattgg cggaaaacag ggtctatttg ctttaggaag
     5821 tctcatcatc aatatcgttt tacttttact tttgttgctc atctttacta aaacagaagg
     5881 gatttctcta ttaaatatat ctatcctgtt cgtcgttata gggatgatta ttagcatatt
     5941 ggctttagat ggatggaata gaagcagtat aatcaaaatt tcagctgcag ttacgagtgt
     6001 ttttttagca ttttttattt gtcttttgac aatgaatcat tggaaagacc aaggactgcg
     6061 ctatgaggaa ctgaattatc tgacacgacc gtatcgttct gtttttcttt ctagcgtact
     6121 gattggaaca gctggaggaa cactcgatac cgttatcaca gtcattgcga cattagaaga
     6181 attgactaag cgacaaccag aaataacagc gaaacagtta tggctttcag gaaggcaagt
     6241 gggtcgagat gtgattggct cgatgaccaa tatccttttg ttcgtctata tcagtgggtc
     6301 agttccatcg cttcttttat atttaggaaa tcaatggtcg ttcaaagaaa cgatagaaca
     6361 gcatttatca ttggaatttt tacgcgtcat tgctggcagc ttaggaatag tattatcgat
     6421 ccctatctcg gtcctctttt ttctgactgt aaggaggctg aaacgatgaa tgtacttatt
     6481 atacttgttg ggattttagc aatcagcgtg ctacttattg gcggtacgca aggtatgtat
     6541 attcttttag gcttattcat caatttaggg atctttttcc tgttgttatt cggttatcat
     6601 cagaagtggc cgatactggc attgtcggtc ataggatttt tattgattgc tgtggttatc
     6661 ttatttttta tcaacggtta taatctaaaa atgcgtgcag cttttgtgag tatactgatc
     6721 tttttatttt gtttcctact attgcttcct atcactgatt ttttagcgat ccaaggtttt
     6781 acatcggtag aactagaaga attatctggc ttagataaga ctttagcgat tgattttaga
     6841 ttacttacga gatcgttgct tctgattagt cttagtggtg ctgttttaga tgctagtgta
     6901 gcaatttcta gtgggacatt cgaagtatat caggcaaatc cgcaattatc tttttaccaa
     6961 ttgcgccatt ctagtttcgc tattgctaaa aaaatattgg cgtcaacagt aatgacactt
     7021 ctttttgcat tcatgggaag ttctttagcg ttgatcctct ggtttattga tttagacatt
     7081 ccttttcagc aagtgatcaa tgaaaaatca tttgttctag aatatacgat ggctattctt
     7141 accacattgt ctgcactact tgttcttcct ttaacctgcg tgactacagc atatctcttt
     7201 acaaaaaaga aagaacaact caagatggag taaaagttac aacttaagtc ctatgacatt
     7261 tttactgaat cagtgcataa tatggatgta aggaaaagct tgcaaacaat tttgcttaag
     7321 gaggcaattt ccatgaaaga aagagaacgt attttagatt tagtgaaaaa aggaatcctt
     7381 tcgactgaag aaggcttaga tttattagaa agcatggcaa ctgaaaaaga cgaaaagcaa
     7441 ataaaaaaag aagcagacaa ggtaactgct agtcataaag aaaaagatca ggcaagtcaa
     7501 ttgatcgata aacttgaaaa cggcgaagaa gaaatttctg agccagttga tccaaaggaa
     7561 agagaaaggc aagatcaaga aaacttagaa aagatcttag atgaactagc aactgaagcg
     7621 aataaaactt cggcgcgtct agatgaagta aatgctcaat tcgctgacaa taaggcagca
     7681 cgcaatgaga aacaggaagc attgatgcag ctgaatacaa aagaagaatt aggcgagttg
     7741 acagaagaag aattagcaca gcgtcagacg ttagaagcag aaatcaaaga attagaagct
     7801 agtggcgata cactgttaga agaacaattg aaattagaag cagaattaaa agatatccgt
     7861 aagaatcaat ggtctgagaa aaaagaaacg tttactgata agtttgaatt gccagatgat
     7921 tggaaagatc aagcaacaga tacactgaat caagtaggcg aaaaaatgtc agaagctgga
     7981 tcacagttag gcaaattctt gaagaaaaca ttccaaactg tttcagaaac agtgaatgac
     8041 aacatggaat ggaaagatgt cagcttacgt gtccctggga ttgcgacaac gaaatttgag
     8101 catgaatttt actatgaagc tcctgcagca agtatccttg atatcaaggc agcaaacgga
     8161 aacgtgacgt taaaaacgtg ggacagcaac gatgtgaaag ttgaagctaa gatcaaactt
     8221 tacggaaaaa tgggtgctga accatttgaa gcattctctg aacggagcca gattgaggtt
     8281 aatgaagatc atatctcctt ccaaattcca aataaacgtg tgcgtgcaga ccttgttttc
     8341 tacttaccta aacgagtata cgaccacgca gctattaaac ttctaaatgg caacatcatg
     8401 attgaaacat tggaagctaa agatatttat acaaaatcta cgaatggtaa catcatcgtg
     8461 gaccaattaa ctgcaacgat gctggaagta gaaggtgtaa acggcaatat cgatattcga
     8521 aacgggaaca tcttagattc tattatcgaa acagtaaatg gtacagtgac atttggtgcg
     8581 acagcagaaa acctttctgt gtctttggta aatggtgatg tacgtcttac gattaaagaa
     8641 gataatttga aaaaagtaga agcaagttca gtgaacggaa atgtcaaagt cgctttacca
     8701 gatggcatcg gtttggaagg acatgctaaa acaagtcttg gaagtatcaa cagccgtctg
     8761 tccaattacg aagtagttcg tgagaaaaaa gaacggacaa accaaatgtt gcaattccgt
     8821 cgtgtttcag atggtgaaat cacgcaagtg caactttcaa cgacaacagg aagtatctat
     8881 ttaaaagata cagataaata aagaggttgt gaaagagatg ttcagccttg agcaataaga
     8941 agcgattttg taaaatagct tcccctattt tacaaaattg tgccttattg ccgataggct
     9001 atctcttgaa caccgtttac caagagatta tgaacctgcc aattatcgcc gagtcacaag
     9061 gaataatttc agaaaaagct cgtcacgttt tatgaatatg gtaatttgtg accgagaatt
     9121 ttgcagaaag acaccgatta agaagaaggt tgtcacaacg attttcttca tttaaaatag
     9181 tagaacagag gtgagacatg atgaaaaaaa agttgacaaa atccaataaa aatgttgttt
     9241 tgacagggac tttagcagga ataggagaat acatcgggat tgatccgact gttatccgtg
     9301 tgctttatgt attcctgagc ttagttgctt ttggaagtcc aatcatttta tacattttat
     9361 tagctttgat tatcccatct gcgcgttctg cacgaacaaa ctatggacat aacaatgcat
     9421 attatgaaaa aaacaattat cgtgaacaaa ctcgtgaaca aacaaaaaaa cgcaaagaag
     9481 cggaaaaaat cgatgatgac gaatggagtg acttttaatg tcgtactttc aacgtatttt
     9541 agttaatacg ttaacattca tatcattggc tgttcttttt cctgcgatgg tccatgtgag
     9601 cagtatttgg acggccgttc ttgccagctt cgtattgtct gtgttgaata tgtttgtcaa
     9661 accgattttg acgatactgt ccctgccctt tacattagtc acattgggat tgtttacgtt
     9721 tgttatcaat gcagcgattc ttaaaatgac ctcctttttt gtgggagcag aacattttag
     9781 tttttcttca ttcggtgcag cgttgttagt agctatcttg atgtcgcttg tcaatgtaat
     9841 tgtcagtgaa cataacagta gaaaataaaa gaaagctgtg ccgaactatc ggcgcagttt
     9901 ttttatttag aatcaggaat gcgttttcta ttacattggt aattaaataa aaacgttttt
     9961 tttaattggt agtacttttg gctagacaat gataaaatga tagagaacac tctaatagaa
    10021 atgaggtagc atgaatgtca gaagttgtaa aagttagcca attagtgaaa aaattatctt
    10081 tggaaatcgt gacaggcgac gagaaaagct tagaccgtgt catcgtaact ggggacatct
    10141 cacgcccagg actagagttg actggttatt ttaattatta ttcccataat cgtttacagc
    10201 tatttggcag taaagaaatc agttttgcgg aacggatgat cccagaagag cgtttgatca
    10261 tcatgagaag attatgtagt gaagatatgc ccgcatttat tatttcaaga ggattaccgg
    10321 ctccagaaga aatgatccag gcagcaaatg aaaatgggat agctgtactg cgttctccca
    10381 tctcaacttc tcgtttatta ggagaattat ccagttattt agatggtcgc cttgcccctc
    10441 gaacaagtgt ccacggtgtt ttagtggatg tttacggatt aggtgtactg attcaaggtg
    10501 acagtgggat aggaaaaagt gaaacagcgt tggagttgat caaacgcggt catcgattga
    10561 tcgcagacga tcgagtagat gtttatcagc aagatgaatt aacggtgatc ggagaaccgc
    10621 ctaaaatcct ggaacatcta attgaaatcc gaggaatcgg gatcattgat gtaatgaatc
    10681 tttttggtgc gagtgcggta cgtggctcta tgcaagtcca gttggctgtt tatctggaag
    10741 catgggcaaa agataagaaa tacgaccgtc tgggctctga agacacgacc gttgaaatcg
    10801 cggaagtggg cattccacaa ataaaaatcc ctgtcaaaac aggacgaaac gttgcaatca
    10861 tcattgaagt agccgcgatg aatttccgtg cacgtacaat gggctttgac gcaacgaaaa
    10921 catttgaaga aagattaagt cgcttgattg aagaaaactc aggcaacgac tagcaaggag
    10981 tgacaatatg ttagcacaaa tcaatcgagt tgcttttgag cttgtaggga ttcctatcta
    11041 ctggtatgca ttgatcatcg tatcaggtat cattatcgcc atgtggctta gcagcagaga
    11101 ggctgttcgg gtaggattaa aagcagatga tgtgacagat tttatgttgt ggggattgcc
    11161 tatctccatt attggtgctc gcttatatta tgttttattt gatcttcccc agtatatagc
    11221 tgatccaatc caaatattca atatccgttc aggaggctta gctatttatg gcggactgat
    11281 tgcaggcgcg atcacgctgt atttttttac taaatatcat tttatttctt tgtggacttt
    11341 tttagatatc gctgcaccaa gtgtattatt agcacaggca atcggtcgtt ggggaaattt
    11401 catgaaccac gaagcatatg gaccagacac gacgcgaagc ttcttagaaa gtctgcattt
    11461 accgcaattc atcattgata atatgtatat cgaagggatc tatcgtcaac caacatttct
    11521 ttatgaatca ctttggagtt tgattggatt tattttgctt ttgtttttac gaaagaaacc
    11581 acaccttttg aaaaaaggcg aagtcctttt atgttatcta atgtggtata gtttcgggcg
    11641 tttctttatt gaaggattac gtacagacag tctttacttt ttaggtgtga tccgtatttc
    11701 tcaattatta tctgctgtgt tatttgtcgg tacattgatt ctatttattt ggcgcagaaa
    11761 aaaaggaaat ttaccggatt atgaccgctc attaggaaaa aatcaacaaa ttatctgatt
    11821 tttagagcgt taaaaatgaa aaaaatttca ggagtggtaa aatgaattat agagtagcgg
    11881 ttttaggacc aggctcttgg ggtactgctt tggcacaagt cctcgccgaa aacggacatg
    11941 acgttcggat ttggggacat cgtgcttcac aagtagaaga aatcaacacc caacacacga
    12001 atcaacgtta tttaccagat cattatctac ccgattccat ccaagcattc accaatatgg
    12061 aagatgcagt aaaagacgca gatgcggtgc tattcgtcat tccaacgaaa gcaatacgtt
    12121 ctgttgcaaa agaactagtg cctaaattga atacaaagcc agtgatcatc catgcaagta
    12181 aaggattaga acagggaaca cataaaagga tctctgacat cttgttagag gaaatcccag
    12241 cagaaaaaag aaaagctgtt gttgttcttt ctggaccaag ccatgcagaa gaagtagcga
    12301 aacatgacat cactacgatc acagctgcaa gtacagatgc aaaagcagca aaatatgtac
    12361 aagagctgtt catgaataat tattttcgga tctataccaa tcctgatgtg atcggtgtag
    12421 agatgggggc agctttgaaa aatattattg ctattggtgc tggcgctatt cacggcttag
    12481 ggtttggcga taatgcaaag gcagcaatca tgacgcgcgg attagctgag atcagccgtt
    12541 taggtgtagc aatgggtgcc aacccgctga cctttatcgg attaagcgga gtaggcgatc
    12601 tagtcgttac ttgcacaagt gttcattcta ggaactggcg cgcaggcaac ttgttaggac
    12661 aagggcagcc cctagaagag gtgctagcca atatgggaat ggtcgttgaa ggtgtgaata
    12721 ctactcaagc agcgatggaa ctagcccaaa taatggaaat cgaaatgccc atcactcaaa
    12781 caatctatga agtgctgtat gaacaaaaag aaatcaaaac agccgcaaaa gaaatcatgt
    12841 tacgtgatgg gaaaaaagaa aatgaattct cattaacaat tttttgaagg agcgaatgta
    12901 tatgaaagtc aaaaaagcag ttattccagc ggcaggatta ggaacaagat ttttaccagc
    12961 aacaaaagct atggcaaagg aaatgttgcc aattgttgat aaaccaacaa tccagttcat
    13021 cgttgaagaa gcgttgaaat caggaatcga agacatcttg atcgttacag gaaaagctaa
    13081 acgaccaatc gaagatcact ttgattcaaa tattgaatta gaaacaaacc taagtgaaaa
    13141 aggaaagacc gaattattga aattagtaga agaaacgaca gatgtcaact tgcatttcat
    13201 ccgtcaatct catccaaagg gtttaggaca tgccgttctt caagcaagag cctttattgg
    13261 aaatgaaccg tttgtcgtta tgctaggcga tgatatcatg gaagatgaag taccattgac
    13321 acagcaatta atcgatgatt acgaacgtac tcatgcttct acgatcgccg ttatgcaagt
    13381 tcctcatgat gaaacttcaa aatacggaat tatcaatcca ggagaagtat tggaagatgg
    13441 gttatacaat gtcaaaaact ttgttgaaaa accagatcca agcaaagcac caagtgattt
    13501 agctattatt ggacgttact tattgactcc agaaattttt gatgtattgt ccgagcaaca
    13561 accaggagcc ggaaatgaaa tccaactaac tgacgcaatc gatacattga ataaaacaca
    13621 acgcgtattt gcccgtaaat tcacaggtaa acgttacgat gtcggtgaca aatttggttt
    13681 tatgaaaaca agtattgaat atggtctaac acatccagaa gtaggtgcac ctttacgtga
    13741 gtatatcctg tctttaggta aaaaattaga agcagaagga agcaagatcg atcataatcc
    13801 aaaaggaata acagaaatca aagaagaaaa gaaaaaataa cccttttttc taacgagctt
    13861 cagctgctaa ttgaataaaa agtagttgaa gctcgttttt atttgtttaa aaaacacgaa
    13921 aatgctatcc ttatagaaaa gagagacaag aagggagttt gatcatgtca ggaggagaaa
    13981 ttgcaggatt gatcgcagca atcgcctttg cgatacttgt cgtttttatc gttcgtgtgt
    14041 tgttgcaagt ttctaaaact ttagaaaaag tagatcagac tgtagcagaa gcaaatacga
    14101 cgatcgaaat cgtcacaaaa gacgtagatc ttttgtctag acaagtagaa ggcttgctgg
    14161 tcaaaagtaa tgaactgctc ttggatatca atggaaaagt cgcaacgatc gatccattgt
    14221 tcacagcagt tgctgattta agtgaaagtg tatcagaact taatcattct agtaaaaaca
    14281 tcgcgacgaa agtcggcgga atcggtaaaa cgacagctaa agcgacggtt gccggaaaag
    14341 tcggtgggac agcgatgaaa ttttttaaaa acaaaaaaca tactgaaacg acaggaggaa
    14401 aataaaaatg gctaaaaaag gtggattttt attaggtgca gtagtcggag gaacagccgc
    14461 agcaatcgcc gcgttattgt tagcaccaaa atctggtaaa gaacttcggg atgaattagc
    14521 tgcccaagcg gatgatttga tggatatggc ttcagattac acagatatgg cgaaagaaaa
    14581 atcgactgag ttaacaggta ttgcaaaaga aaaagcgcaa gatctttctc aacaagcagg
    14641 tgatctagca ggtagtgtca aacaaaaagt aagtgaatca gcagatgaag caggaagtga
    14701 atctgacgat atcttgttaa atcttaaaga acaatcagct gacttatcta atcgtttcaa
    14761 aaaaacggca gaagatatga aaaaacctgc agatgaatta ggaagtattg ctaaagatac
    14821 ttcagaagat atcatgattg atgtaaaaga cgctgctggt aaagcaggag atactgtcaa
    14881 tgaaggaatg tcagaagcaa aaccagttgc agatgacaca aaagaaatga tgaaaatggc
    14941 aaaagaagat gcgaaagaag ctaaaaaaga aataaaagat acaatgacag attcatctac
    15001 aaatcactaa aatcactaaa atcacaaatt tatagaataa aaaggtggga gcggactagc
    15061 tctcaccttt tttactctta tttttctaaa actagtaatt cttttggtgt agtcgtgaat
    15121 ggcacacagc catcgcttgt cacatataca cagtcttcga tacgtacccc tactttttca
    15181 ggaatataga ttcctggttc aagagagaag cacatccctt cttcgattac taagtcattt
    15241 ccttctacaa gggaagggaa ttcgtggacg gttgttccga tcccgtgacc taaacggtga
    15301 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    15361 cctgctgtca cacctggacg gacggcttct tgtgcggcaa gctgtgcttc aagagtgatg
    15421 ttgtatattt tttcttggaa gtcggttggt ttttgataag caattgtacg agtagcatca
    15481 ctgcaatagc cattccaaac aacgccaaga tcgaataaca caagttcatt cggtgaaatc
    15541 ttcgtgtttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    15601 tcgaaagaca tttgtgaaac accttgtttt ttcaattggt attcgatttc tgcaatgatc
    15661 gcttgttcag tcacgccttc tttcactgct ttaaagccaa tctcaaatgc aacatcggcc
    15721 catttaccag cttcgatcag tttgtctttt tcctctagag ttttgatcag ttgtagacgt
    15781 tggatgatgg aagctagatc agtgtaagaa tccgttcccg tcaagatatc agagatagca
    15841 tcgaagcgct cgacagtcaa ggcgttcttt tcaatcccta cattgccagg ctgaccatag
    15901 cgagcacgaa gttcttttcc gatgatctcc caagggtttt cgctgtctaa atagccacgg
    15961 acatcatacg cccaactact gttttgagca tcttcaactt ccaaagcagg agtgaaaagg
    16021 aaaggatctt gttcaagagg gataaaaaga gctagtacac gctcatgagg atcactttcg
    16081 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc gacttggtgt
    16141 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    16201 taaattaatt ttgtattcaa taattgtacc actgatttat taaaagacca acgttctaaa
    16261 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttcttgagat
    16321 tatttgatat tctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    16381 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    16441 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    16501 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaacg cagttgcgcg
    16561 tggcttggca agtaagaaaa caacaacagt tggggtaatc atccctgatg tcagcaatat
    16621 gttctttgct tctttagcac gtggtatcga tgatgtggca acaatgtaca aatacaatat
    16681 tatcttagca aattcagatg gtaacgacca aaaagaagtc aatgtattga ataacttact
    16741 ggctaagcaa gtggacggcg tgatcttcat gggtcaccat atcacagacg aaatccgtgg
    16801 agaattttca cgttcaaaaa ctcctgttgt attagctggc tctattgatc cagatgaaca
    16861 agtaggtagc gtaaacattg attatgtatc agcaacaaaa gatgcagtaa acaaattggc
    16921 aaaaagcggc aataaaaaaa tcgcttttgt cagtggtgcg ttgatcgatc ctatcaatgg
    16981 acaaaatcgt ctgaaaggct ataaagaagc attaagcgaa aataacctac catatagcga
    17041 aggattgatc tttgaagcac aatacaactt caaagatggt ctatctctag tcgatcggat
    17101 ccataatagc ggtgcaacag ctgtctatgt ttcagatgat gaattagcca tcggtatttt
    17161 ggatggttta ttagaccgtg gcgtgaaagt accggaagaa ttcgaaatca tcacaagtaa
    17221 caattcacta ctgacagaag tagctcgtcc tcgtttaaca agtatcacac aaccattata
    17281 cgatttaggg gcggtatcta tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    17341 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatctacaa aataataaaa
    17401 ggacaaccat tcagaggttg tgagcctgcc gtttaactct gcgttacaag gagcaatttc
    17461 aaaaaacagc atttcatgtt tgatgaatat tgtgactcgt gattcggagt tggcaggaga
    17521 acaccgttta ttcgggaaaa ggggttatga caaaattttg tttaatcctt tttttttgtc
    17581 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    17641 gatgctgcat tatgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    17701 cagtccctta tt
//
LOCUS       DACJGB010000064        17232 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.064, whole genome shotgun sequence.
ACCESSION   DACJGB010000064 DACJGB010000000
VERSION     DACJGB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.064"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(97..1116)
                     /locus_tag="IYI34_002217"
     CDS             complement(97..1116)
                     /locus_tag="IYI34_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0560062.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="IYI34_002218"
     CDS             complement(1138..2145)
                     /locus_tag="IYI34_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAR0560063.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IYI34_002219"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IYI34_002219"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAR0560064.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="IYI34_002220"
     CDS             complement(2796..5090)
                     /locus_tag="IYI34_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAR0560065.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="IYI34_002221"
     CDS             5350..7515
                     /locus_tag="IYI34_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560066.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="IYI34_002222"
     CDS             7819..8652
                     /locus_tag="IYI34_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAR0560067.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="IYI34_002223"
     CDS             complement(8754..10454)
                     /locus_tag="IYI34_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560068.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="IYI34_002224"
     CDS             10961..12121
                     /locus_tag="IYI34_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR0560069.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="IYI34_002225"
     CDS             12137..12580
                     /locus_tag="IYI34_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560070.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="IYI34_002226"
     CDS             complement(13063..13593)
                     /locus_tag="IYI34_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAR0560071.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="IYI34_002227"
     CDS             13698..14249
                     /locus_tag="IYI34_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR0560072.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="IYI34_002228"
     CDS             complement(14402..14875)
                     /locus_tag="IYI34_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR0560073.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="IYI34_002229"
     CDS             15013..16485
                     /locus_tag="IYI34_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR0560074.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="IYI34_002230"
     CDS             16482..17192
                     /locus_tag="IYI34_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAR0560075.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5356 a         3097 c         3543 g         5236 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggagaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gagttttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataagt actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagcca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacacccacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa
    15361 gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg
    17221 aaaaagctgc tc
//
LOCUS       DACJGB010000065        16875 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.065, whole genome shotgun sequence.
ACCESSION   DACJGB010000065 DACJGB010000000
VERSION     DACJGB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.065"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IYI34_002231"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IYI34_002231"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAR0560076.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IYI34_002232"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IYI34_002232"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAR0560077.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IYI34_002233"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IYI34_002233"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAR0560078.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IYI34_002234"
     CDS             complement(4028..5044)
                     /locus_tag="IYI34_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAR0560079.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(5146..5343)
                     /locus_tag="IYI34_002235"
     CDS             complement(5146..5343)
                     /locus_tag="IYI34_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560080.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(5315..6634)
                     /locus_tag="IYI34_002236"
     CDS             complement(5315..6634)
                     /locus_tag="IYI34_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAR0560081.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(7375..8355)
                     /locus_tag="IYI34_002237"
     CDS             complement(7375..8355)
                     /locus_tag="IYI34_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAR0560082.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(8368..9246)
                     /locus_tag="IYI34_002238"
     CDS             complement(8368..9246)
                     /locus_tag="IYI34_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAR0560083.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(9262..10062)
                     /locus_tag="IYI34_002239"
     CDS             complement(9262..10062)
                     /locus_tag="IYI34_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAR0560084.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            11454..11780
                     /locus_tag="IYI34_002240"
     CDS             11454..11780
                     /locus_tag="IYI34_002240"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR0560085.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            11788..12696
                     /locus_tag="IYI34_002241"
     CDS             11788..12696
                     /locus_tag="IYI34_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR0560086.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            13325..13576
                     /locus_tag="IYI34_002242"
     CDS             13325..13576
                     /locus_tag="IYI34_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAR0560087.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            14118..14276
                     /locus_tag="IYI34_002243"
     CDS             14118..14276
                     /locus_tag="IYI34_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0560088.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            14348..15574
                     /locus_tag="IYI34_002244"
     CDS             14348..15574
                     /locus_tag="IYI34_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0560089.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="IYI34_002245"
     CDS             complement(15663..16055)
                     /gene="rpsI"
                     /locus_tag="IYI34_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAR0560090.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="IYI34_002246"
     CDS             complement(16071..16514)
                     /gene="rplM"
                     /locus_tag="IYI34_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAR0560091.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         4931 a         3597 c         2885 g         5462 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaactt aagaaaacca
     5101 ccggagaatg aattctccgg tggacttttt gtatgattaa ccttcttatt gaacatcact
     5161 gatcttaaac tctattccaa gggcgtttct ataatattct ggatacatct ccttcccaca
     5221 tttttcacag gtaaattggg gaggattgtt cggatcattt gcaatatctg catcatctaa
     5281 aaattttact acatccattg ggattgattc tttttcacca caacttagac atataaattt
     5341 cacgttggcg ttcccaatcc cagcgaattg ctttaaaagt tttttctttt tcttcttcat
     5401 actgttgcct gaattctttt tggtcaattt ttcgattctc ctctcgcata aaccgctttg
     5461 catggttatc tttcgcatat ttaataacta accgtccatt tttgcgcaca ctcatcccaa
     5521 cgaattcata gtactcaccg caacaagaac actctaaggg attttcacca aattcttcgg
     5581 ttattctttc ttcccaggat tttggtctta aggcctcatt cagattgata aacagctttt
     5641 ttactcgttt ctgatactcc ttcatgacct ttttcattaa tgttttgatt ctacgactat
     5701 agattccata gcgacgaatc gttttgaaat tcttattttg aatatggcga atcagtgcag
     5761 ctatgaattc gtctatactc atggtcatcg tctctttagt attcgttcgt ttgtccatgt
     5821 agttgaacat gacagtgtcg ccatcataca ttaagattcg gcttaatgcg atcgggccac
     5881 gcttcatata acgactgatg tattggagta gtgactttag atttgtcctg ctccttttag
     5941 gagcatttac gtaaaaccct tctccattgt tcttgtacgc agcttgtaat cttgattgaa
     6001 ctctttgctt gtcccactca cctaacgttc gtctaattag tttcaataca gcatttttcc
     6061 agtattttct taacatagaa aaaggtaaat aatcgtattc ttcccaactt ccatctcttg
     6121 ttaagccacc catagtaaca accatatgaa catgcggatt aaattctagt tttgatccaa
     6181 aggtgtggat tgtagctact atacctgcct tcacctttct tttctcaaaa taggcaagaa
     6241 taatttttgc ggcttcatcc attagtcctt tcagtaagga atctcgatag ttttccatca
     6301 taaagatttc ccgtaacccc tcatcgatcg taaagattac gtgccgatgt gtcactttaa
     6361 acatatcatt cgccacaacc gctgaccacc tttggctttc acctgttgca cacgttggac
     6421 agaactttcc acgacatttc aagggaacaa actttgtttg atgacatccc tcgcagacaa
     6481 acagtcggta tcctttttga atatctccac aatatctaaa cttgtttact tcccgtttga
     6541 cgactagccg aatctttcgc ccgtatttct caacaaagtt atcccaatta tggtgttcgt
     6601 caaaaaagat tgcttcgaga atattcgctt tcatagtaat attttacttg ttcaaaaaca
     6661 aaatgaaaag ggctaaaaga ctggtagtgc agccttttta gcccttaact ttttatactt
     6721 tctttactgt ctaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
     6781 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct tttctttcag gaagacttcg
     6841 gtaattttta ggagttctac ctattctttt aaccgtttat tctctttttc taagcgtgta
     6901 atttcatttc tgcgaatgga tttcatgact tcctctaccg agaaaagtac gttatccata
     6961 tttttcaact tcttgaactc atcaatacac gctattagag cgagcttcta gctatagact
     7021 aacttcttta acagaaaacc cttcttccgt taccagtttt acagcggtaa ttttaaatat
     7081 ttcatcaaat gaacgtcttg gcataatcca gatcttcgct ttctcttaat ttggtgtcaa
     7141 ttttagtata acccgtccaa cttatttttt aactaagtgc ccggtttttc ttgacaactc
     7201 cacccaaaag ttagattttt tggtctaagt tttagggtcg cttcattact agaattcaac
     7261 aagagatgtt ttttatttta tttaaattat ggggtcagtt tccatttgat tacaaacttc
     7321 tttgttttct ttttttaaac gcaataataa aggttttaat ttcagccttt ttaattagaa
     7381 aatcaatttt cccaaattat tttctttagc tttcttaacg atttctgtcg caactaccac
     7441 atctaataca gccgaaccaa ctgttttgaa gatcgtgatt tgttctccac ttgtccgacc
     7501 gatcaattct tcattgatca gttggcctag ttccccatga tagctgtctc tttgaatata
     7561 cccatcttgc agcggtgaaa tgaagtcccc agcttctttt agaacaccgt ccatggtatc
     7621 aaaaataaca acatctgcgg ctttgattat ttctcgtgga atttcacaca tttccggtgt
     7681 gtaagcaccg actccattga tgtgcgcacc ttttttgacc cactcagcag agaatgtcgc
     7741 acgttttgaa gtggtcacac ttgtaatgat gtctgctcct tcaacgcatt cttgactggt
     7801 ttttgttggg cgcatcgtta cagaaaattg ttgcatcatt ttttcagcaa attggctggc
     7861 acgctcaaaa tcaatatcaa aaatccgaac ttcttctaat ttacgaacgg tcaacattgc
     7921 ttctaattgt gactgtgctt gtccgccggt tccaatcagc gcgccaattt tagcatcttc
     7981 tcttgcaaga agttctgttg ctgctccttg aacagcgcct gttcttaatt gtgtcaaata
     8041 agtaccatcc aaacaagcgg ataccatacc cgtttctgga tccaatacga tcatcgttgc
     8101 tggtacactt ggtaagtttt tcttgatgtt ttcaggatac acggaaacga ttttgacacc
     8161 tagcgcgcct tcgccacctt ctacataagc aggcatatat aagctttgtc cgttgctttt
     8221 tggcacatcg atattggtgc gaagaggcac acttgccttt cctgctgaat acaatgacag
     8281 tgcttttttg tccgcatcga tggcttctct cattgaaaaa cttttgataa tatcttcttt
     8341 tttaagaaat aacatttgaa tcgctcctta attagctgct gtaacttttt ttaattgact
     8401 gcccaacatc gtatatgcac tgtatagtaa gaccaaaatc acgatccatt tcaggatata
     8461 ggtatctaaa gatttgacta ggaatacagc gaccaatacc cccaatacac cgaaggttga
     8521 agtaaaaagt gtgatttttc tgctgtattc attaaattta atgaattgaa cactgccgat
     8581 cggtactgag aaggtacaag cccccatcat aataggaaag gctgcaactg gattcaagcc
     8641 tagcaagtag acagttacca tcgttaacgc ataagaacca atgccgatat tgttcaatgc
     8701 accatagaca aagagtaaaa atcctgccaa gattaatttt ccgccataca attcactggc
     8761 tgttccgttt gatggaatcc agttcatttg tcctgcaaaa atcaacaatg cagcaatgac
     8821 caatcctatg ccgacaaaca gtttgatcgt tttctctggt aacttcacga caaatcttgg
     8881 tcctaaatat gcaccgataa tttggcagat aatacaaaca gctagtgtct tgatccctac
     8941 gttgatggca gtgatgtaag acaatgccat gacggctacc ggtactacac attgggtatt
     9001 caacgttccg ggtagttttt tcatgctgac ccatttcaac tttggataaa ggacggttcc
     9061 gatagcaaag tcggaaacgc caaatgttga aagaaagaag atgataaatg acgtgaaggg
     9121 caacagacgc atatcccctg gttcttcttt gaaattttgt ttgtttttga aaaggtctct
     9181 tagaaagtaa ataataaaat atagattgat tgcgacaatt agtgtcagca aaatattccg
     9241 aatcataaaa aattcctcct actagtaaat ctttctgatt ccctcaaagc cctcaccata
     9301 ttttgcttta agttcttcag gagattctgc aatcactttt agtgtatatg catcatgttt
     9361 gacatattct tttgctagat tgaccatggc gtggtttttt gtccaagtga catcggttga
     9421 ttgcagttct tcgttaatgg taccaaacac gacttcttgc tcatcggcga cgatgttgat
     9481 ccatcttgct ccaaagtcag caagtttgtc tgtttcaaaa ccatgaatat aatacttttc
     9541 taagggaaga tcatattcat gggtagaact gaataaaatc agtacaaact tatcaactct
     9601 tttttcagct tgctgcaatg cctttagtag tgaatccgtg aaattctcat gccaaacttg
     9661 aatgagcaaa ctttcttttg cgtttttcac caaatgctct actttatcca gaacatactc
     9721 gataccatca actttccaca gcatttcttc ttcatctttt tctttgatca tccctagatt
     9781 cttggtaaga aaagaaagat catttttaac acttttttct aatttctcta aaaactcatt
     9841 tgccgagatg gcatgataaa gtttaggttc tgatttatta acgacaatca aattcttttt
     9901 taatagtttt tccaaaatat tatatacttt cgagcgtggc acacctgaga ttttacttac
     9961 ttcatatcct gtaagattac ctttttctaa tagtgtggta tacactcttg tctccatctc
    10021 tgaaaaatca tattttttca tgatttgaat aatcgtttcc attgattaat cctcacttta
    10081 tttttagtag ctactacggt aacaactata aattcttttt gattatctgt caagagttag
    10141 ttcaataaca cacatataat aattagctta aaaacaatct ttttattaaa aagaaacatt
    10201 ataatatgaa tgtttgtgaa aaaataatac aacttagtca ttctttacaa cgatttatag
    10261 aaaaacaacc tctttaaata taaaaggtac gggttactga ttacttgtaa atctttacgt
    10321 tccagtttta ttctttctaa aaaatatact cacttctttt cttagacttc tttttttgat
    10381 tctcctcttt atggtcttgt ccttttttgc cttcaaaata tttaaataaa cccctttcaa
    10441 aagcatttct aggttttgga caataaggaa tgactttttt cttcatataa atacctcatt
    10501 taactatttg atatcatcac attatcaaaa aaaataggtt ttttattaaa actatcttga
    10561 cctttcggga gatttcactg tgatttattt ttattcccat acttttttcg ttggtcactt
    10621 tcgtatacct aatctaagcg atcatcggcg acggtgcttt tacaacgaat ggcaggacaa
    10681 aacaaggggc ggaaaaactt ttttgcctat ttcataaaaa actttttcct acagcaatcc
    10741 cttgttttgt cctaccacac gttgtgaacc caaagcacgc cgacgaacgc ttagctatac
    10801 gaaaatgacc tccaaaaaca aaaaagtgag gtgaatagaa acaaaaagaa attgaaatct
    10861 cccaaaatgt cgtccgcctc ggagacaact tgctgctgaa aatggttggt taattcattt
    10921 aagtattaaa taagataaat catcaaatta atcggaaaaa tagcatgatt ggccttatca
    10981 aaaataaact gtaaaaactt atatttctat gagtggtgga acaatgctaa taccaaatta
    11041 atttatcgat tacccacctg cacaaaccaa agatagcatt attaggaaac gaatcaatca
    11101 gcgaatccag cattataatg aggatactaa aaataaagtt ttcataatta taaatccgcc
    11161 ttcctacgaa aggcaccaat gaggtgatga aaaccttaga gcgctttctt tcttttttta
    11221 ttccgtgtta ccctttcctt gcaggtccct ggtacactac agaacccgag gaggtgagtg
    11281 gcggggctgt atagcggcaa ccccgcataa ttatatggtg tcggtcatcc gttaaggcat
    11341 caatgaatgg cgcaaacccg tagcccgtaa acttatctct tccgaagttg actcgatttc
    11401 gccggatgac cagtccctac caaacctgta ctattttttt acaggaggtt gctatgttca
    11461 aaatctttcg taaaaattgt tgtggcttgg atgtccacaa aacgtggatc tatgcgtgta
    11521 tcggaatcac agatgcgaat ggtcgtacag tgtataagca agctcgcttc tcttccttta
    11581 caaaaggtct caatgaatta gctgaatggc ttgctaaata caactgtaag gatgtgtgtt
    11641 tggaatcttc cggtaaattt tggattcctg tttttaatat tcttgaaaaa acttgttttg
    11701 tcacactggc tcatccgaag tacaccaaac cccagaaagg aaataaaaca gatcgcaaag
    11761 atgcaaaaat ggatttgtga tctctttatg tgcgacatga tcaaacccag ttttattcct
    11821 ggtcttgaaa tccgccagct tcgtgatcta atgcgttatc ggacgaaact gaccaacatg
    11881 aaaacaagtg aaaagaatcg tgctttaaac tgcttaaccg tttctaatct gaaactagat
    11941 gatgttttta gtgatgtgtt cggaaaatca tccacttcta tcatccaata cttgttagaa
    12001 catcccggtg aatcatttga tgttcgacca tttatccatg cccgttgtaa aactcccgtt
    12061 gaagaaatcc aagcggcagt tgagggggcg atttctgtac ctcaagcgat taagcttcgc
    12121 caatgcctca accatattaa cgagattgag aagcatcgag aagagatcaa tctagagatt
    12181 caagctcttg cagaaccctt ctcccctgtt ttggaactac ttcgtaccat accgggctta
    12241 gacactcagc caataacagc gattgcgatt ctttcagaaa taggaccgga tatgtccgtg
    12301 tttccaactt cgaaacactt tatttcttgg gctggatgct gtccaagaaa cgatcgcagt
    12361 gcccgacgaa caaaatcaac acgtatctct cgtgcagggc agtcgttgaa accgctactt
    12421 gtacagattg ccaatgctct tctcagatcc aaagaacatc cagaatttaa aaatcgctat
    12481 cgtagaatca aagcgaatcg tggacacaag aaagctatca ttgctatttg taagatgctc
    12541 ctgaccgcta tctggaatgt cttatcaaag gtggaaccct attctgctaa aggatatctg
    12601 gagccaaaac ctgcgaaaga agagaaagtt ctgacgcaat cccaagctct ggacctttta
    12661 agaaaacgcg gatataccat tatagatagt gcttaaacca aagttacgtt gattcttcct
    12721 aaatagactc gttgtcttcc atttcaaaaa ttttgaaaac ggtaggctta tttagtgtac
    12781 cgttcttcag gtatcttcaa atagatttat tttcaaactt atttcctttc ttcttttact
    12841 ctcctatagc ttagcattta agtgggcact cgtgtcacct tgctagatag ctatgaatgc
    12901 cgtaataagc ctcctagtat ataggcagac tgccaattat aactgattca atccacatta
    12961 tgataaaatc caccttttcg tttcatatgt gttgaaatat cacatatgtc aaaagtgcac
    13021 gagtcttttc attcagtgct ttgtaaatac tcaaaattat tccttttcac tttaaaaaaa
    13081 agataattag tacaaaatca gaaatgagga aagtattagg atggactaga aaggagttgt
    13141 tgaatttgtt gcaacagatt ggtcatttat caattaaaaa ttaggtgaag ttttattcat
    13201 cactgcagct gctgtttccc agagaataaa acgtctagaa gaaactggtc agttactgta
    13261 ttatcaagca gcaattgact acgaacaagc ttgattacta accaaagcgt ttatccacct
    13321 gttgattaaa tcaaatcaaa aatcaggttt ttatacgttc ataaaagcaa tctccagtgt
    13381 actcgaatgt gactgtatca taggtcctta ttcgatgtta atcaaaatgg ggtttccttc
    13441 taccgaatca ttggacagtt ttatcaatga tttatcaaag tttggacgaa ccaacaccca
    13501 aatcgttttc tcgactcttg tttccttaat ggtttatcag ttctggaatg tttatcatgc
    13561 gcttttatta aaatagaaaa gagctttttt catgcgacaa agcagtgatc atttattggc
    13621 acatgcaatc tttgtggatc cattttttga aaatacgata tttagttttg acccaaaaat
    13681 ctttaaatac gtatttgata atttatctta acaaccagat aattttattc agcaccgtat
    13741 tatctatatt gaattctcgg aatcaagata aatttttttc cgtttttgtt tcttttttaa
    13801 tcttcttctt gttattaata gtgattagga tgatattcct atattgatct ttgataatta
    13861 gatataattt ttgaataacg ttccctgcta ttcgagctag aaatacaagt acgccatgac
    13921 ctagttagcg acaactactt agagatttct tttgttctgg gacaacaaaa tgccatgaca
    13981 atagcagtaa ataatgatac ttccgtaagg ctcgccgtaa actggggaga ggtgaaattc
    14041 ctatgtgctt acccaaagcg gttcaaaaat tttaaactat tattttagga gatgatttta
    14101 taaaatcgaa agcctacttg aataaaaaac aagcctgtga atttctagga attgataata
    14161 atactttaga taagtggatt cgtgtatata attttccagt aataaaaatt gatggcgtag
    14221 ttcgctttga cgtagaggat atcaaagatt tcatgaatac attcaaaaaa aattgaaatt
    14281 ttcttctcgt cacatatact ataaatagcg aaatgtactg ccttactttt taaaggagtg
    14341 aggaagtttg cctacaaagc tttcaaatgg gaaatataaa actaatctga ggtatccaaa
    14401 aaaattcaga gaaatcactg gaattgcatc agagaagtat caaaaagtat ttccaactag
    14461 gcaattagct attaaggccg aaagggaaat gaaaaagaag attgaaacag ttttaagaga
    14521 ggagaatgca aactcactag aattgaaagg aaaaataaaa tttaaagatt tttatgaatc
    14581 aaaatggctt cctagatatg aactaggtca aactactagg tcaaacaggg ctccaagtaa
    14641 tgtaacaatt agtaatacta aggatatatt ccgattgcac atccttccaa tgtttggtga
    14701 atatgcaatg aattatctga attcaaatac tgagataata tcagatgaat taacaaagaa
    14761 atcgaaagaa tacgcaaata taaaaattat caaaggctat gttcgctcta tgtttgatat
    14821 tgccgaaata ctatgttata tcgaattcaa ccgaactacc aaaattattc aatgtattac
    14881 cgcgcctaag aaaatggctt tagaagaaaa aaggataagg gaagggaatc aagcattatc
    14941 atcagaagag ctgactgatt ggatagaagc agtaaacaat gacttaaata atcacttgct
    15001 tacacttcac gattacacac ttttcatgct taccttatat ctaggtgata gaaaaagtga
    15061 gacttatgct ctgcaatgga aatatattga ttttaataaa aatacggttc gtctcaaata
    15121 tgctttagat aaatatcaaa gaaagacaca tacaaaaggt tggaaagaca ccgttattca
    15181 ggcaccagaa gttgtgatga cattattaag agaatggaaa tcagctcagg ctgaacaatt
    15241 gttgaaacta aaaattatac aaacacctga ccagtacctc tttacctatt ccaaaccttc
    15301 tggagaagta aattgtcctg ttcacgctga ctacttaaac tatcgaatca atgcaataaa
    15361 aaaaaggcat cctgaattgg ctcaattatc tccacacaaa cttcgtcata cctatgcaac
    15421 tatagctagg gaaggtggag caaatatgaa tcaaatctct aatgctttaa cgcactcgga
    15481 tatttctact acaaaaatat acgtgaacac acctgacgta gttgataaat ctgtttttga
    15541 agcatttcaa aaaggattga ataagtgtga ttaacatgtg ttctcaaatg tgttcttatt
    15601 ataaaaaaag acactttcca aataattgaa aagtgtcctg aaatattgat ataaaaagaa
    15661 tattaacgtt ttgagaattg tgaagcttta cgagcttttt taagacctgg ttttttacgt
    15721 tcgaccatac gtgcgtcacg agtaagtaag ccagcacgtt taagagctaa acggaagtca
    15781 ggatcaactt ctagcaatgc acgagcgata ccatgacgga tagctcctga ttgtccagcg
    15841 tatcctccgc cgtttacgtt aacaaatacg tcatatgcgc cttttgtttc agtaacagcg
    15901 aaaggttgat tgattacttc acgcaagtca gcatgtggaa tgtattcttc aacatctttt
    15961 ttatttacag taattttgcc agttcctggt actagacgta cgcgagcaac tgcgttttta
    16021 cgacggcctg tgccgatata ttgaacttgt gccaatgaaa ttccctccta ttagattaag
    16081 tttgtgatgt ctaaaacttc tggttgttgt gctgcatgtg gatgttctgc tccaccgtat
    16141 acgttcaatt ttgtaaattg tttgcggcct aaagtgtttt taggaagcat accttttaca
    16201 gaagtttcga tcaaacgacg agaattttta gcacgcaatt cgccagcagt gattgatttc
    16261 aatcctcctg ggtagttgct gtgacggtaa taaattttgt cagtcgcttt tttacctgtt
    16321 aatttcactt tgtctgcatt gatgacgatg acgaaatcgc cagtatccaa atgaggtgtg
    16381 aaagttggtt tatttttacc acgtagtaca gatgcaacaa ctgttgaaag acgtcccaat
    16441 ggaacatcag ttgcgtctac tacataccat ttacgttcta cttcgcctgg tttggccata
    16501 tatgttgtac gcacgttatt ttcctccaat ttaagttctg atgtttgaca tacacttcga
    16561 gtttccgggg ctctttgtgg ggcaaacaat accatttaac atattacccg ctaatctagt
    16621 caatgtcaac gtttttctgc ttgattgaaa aatttatatg aaactttact tcttttcttt
    16681 cttttatata aaagggtcgt ttcctgtcaa aatgaaaaac aaaaagaggc tgtgacaaaa
    16741 gtcttgtcac aaccccttat tttgaataaa cggtgttctc ctgccaactc ctcggtcaca
    16801 agtcacaata tccataaaac ataagaagct gttttttaaa attgtttctt gtgactcgga
    16861 gctgaacggc aggct
//
LOCUS       DACJGB010000066        16726 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.067, whole genome shotgun sequence.
ACCESSION   DACJGB010000066 DACJGB010000000
VERSION     DACJGB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.067"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(39..1241)
                     /locus_tag="IYI34_002247"
     CDS             complement(39..1241)
                     /locus_tag="IYI34_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0560092.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            1358..1873
                     /locus_tag="IYI34_002248"
     CDS             1358..1873
                     /locus_tag="IYI34_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR0560093.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2072..2518)
                     /locus_tag="IYI34_002249"
     CDS             complement(2072..2518)
                     /locus_tag="IYI34_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAR0560094.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IYI34_002250"
     CDS             complement(2565..4043)
                     /gene="msr"
                     /locus_tag="IYI34_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAR0560095.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4397..5026)
                     /locus_tag="IYI34_002251"
     CDS             complement(4397..5026)
                     /locus_tag="IYI34_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAR0560096.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVTRDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5010..5474)
                     /locus_tag="IYI34_002252"
     CDS             complement(5010..5474)
                     /locus_tag="IYI34_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0560097.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5961..7229)
                     /locus_tag="IYI34_002253"
     CDS             complement(5961..7229)
                     /locus_tag="IYI34_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAR0560098.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7460..8197)
                     /locus_tag="IYI34_002254"
     CDS             complement(7460..8197)
                     /locus_tag="IYI34_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0560099.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8264..8833)
                     /locus_tag="IYI34_002255"
     CDS             complement(8264..8833)
                     /locus_tag="IYI34_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAR0560100.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            8961..9833
                     /locus_tag="IYI34_002256"
     CDS             8961..9833
                     /locus_tag="IYI34_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR0560101.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            10175..11047
                     /locus_tag="IYI34_002257"
     CDS             10175..11047
                     /locus_tag="IYI34_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0560102.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11044..13002
                     /locus_tag="IYI34_002258"
     CDS             11044..13002
                     /locus_tag="IYI34_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560103.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(13253..14104)
                     /locus_tag="IYI34_002259"
     CDS             complement(13253..14104)
                     /locus_tag="IYI34_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0560104.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14227..14694
                     /locus_tag="IYI34_002260"
     CDS             14227..14694
                     /locus_tag="IYI34_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAR0560105.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14957..15439)
                     /locus_tag="IYI34_002261"
     CDS             complement(14957..15439)
                     /locus_tag="IYI34_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR0560106.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15539..16402)
                     /locus_tag="IYI34_002262"
     CDS             complement(15539..16402)
                     /locus_tag="IYI34_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR0560107.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         4990 a         3470 c         2981 g         5285 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt tttcaaaccg atccgactag ataagaaacc tgtaaatagc
     1021 atcaccacac cagtgactaa catgtaacta gtaaataaca gggtcgtttc agctggcgta
     1081 gcgtttaact gtgcagcaat cgtttttaag attgggtcta ctagacccac tcccataaaa
     1141 gcgatcatac aggtagctgc tacaactagc gctgaacttt tcccctttgt ttcttcattt
     1201 gtttgttctt gtatatctat ttgattctcc aatatttcca tccctttctc tctattagca
     1261 tcatacatga tttattttac atgtaaatat gcacatgtaa aacgtggaaa ctttactgtg
     1321 atataataaa gaaaataaga cgagaggtag gatttttatg aatactttag cctatatatt
     1381 attatgcgtg cttttatcca aaccacgttc tgggtatgaa ttgaagcagc tgatcacaat
     1441 tttttgggaa gcccatcaca gtcagatcta tacaacgctt gccaagttag accagcaggg
     1501 ttatgtgacc gttttggaca aagacgagca ctctcaaaaa aagatctacc acttgactga
     1561 tgaaggacgt gctcttgtag aggaatggat caaagaggaa actcccgctc ctacgcaaaa
     1621 agatgaattt ctagcaaaaa tctatgcttt ttcgacgctc gataaaacaa cagctactgg
     1681 actgctattc actagagaac aacagctgaa caaaatcatg caaaaaaatc aaaaaaaatt
     1741 gaatgacctc tcgctagaca agaaacaaga atttggacgt tatgttgtga tccagcgcag
     1801 aattatgctt tgtcagcagg aactccaatg gtgtcagcaa gtaagagaac aaatgcagca
     1861 gttttttgag taagggataa acagaacctt tctagaaata tctattgaag agcaaattat
     1921 cttcaataaa agagcaatac agttggaaaa gaaaaagcga tccgaacgac aggccaaaac
     1981 ctatcgttcg gatcactatt tttctagaac tgcattcaag agactatgac aaaaagcatg
     2041 ccaaggcctt ttttcaaata acagtgttcc atcattcatt actttgaatt gcttttagca
     2101 cacttcttga aagcgggcca acgatgatca actgcaacgg taaagcggca atcacattca
     2161 ttttccaagc attcatataa gcagagaata attgatcagg tattccttgt tccatgatca
     2221 caccgaataa agacatacat gtcaccattc ctaaaaccat caagcatgaa atagttaaga
     2281 tcatttggac cttgctttct ggatttactg gtagtttaaa ggcaattttt ttcgccagca
     2341 caccgatgat gaagacatcc aaaatgaaag ccaccacgaa tccaggcact agaccaatca
     2401 acaaacttga taaatgaaga tcttgatgca gccataaatt atacgcgctc atccccaaga
     2461 ccatcaaaaa acacatcatt gttgtaaaaa ttattccttc ttttttattt gtaggcaatc
     2521 tgtaccactc ctttaaattt gtcacgaaaa tcatcgtagc acatttaaaa atttctcgta
     2581 agtacttttt ttattttctg cccttttatt tcataaactt catcagccgt acgctcgaca
     2641 aagtaagaat catgagaagt aaacaaaatc gttcccggat atatttgcat tagcttctct
     2701 aaagcttcga tcgttgccat atcaataaaa ttagtcggtt catctaacag caacacatta
     2761 cttggcttag taaaaagcaa agcaatcgtc agacgggtcg cttctccccc acttaatgta
     2821 cataacggtt tcgtcaaagt ttcctgagca aaccctaagt gattcagcaa actacggaca
     2881 agcgattctg aaaactctgt ttgtctcatg acaaaacgaa tgatggattc ttcagatgtc
     2941 atttgataac ccttttgctg atatacttga aagcttactt tcggagagag aaggattcct
     3001 tctccttgtt tgcggatatg ttctaataag gttgtcttac ctgaaccatt ttcaccgaca
     3061 atcgcgattt ttttacctaa agaaaactga aaatcaccat ttaccagtaa tgttctccca
     3121 cttctttcca attggacatt ttgtcccatg atgggatagc ggctgtggag ttcaagagat
     3181 tttggtactg gaaaacggat ttgtttcatc tgctgcggct tggttgtttc tcctattttt
     3241 tggagtcttc tctctaatgt tttcgcattc ttttggatgg ctttttgtac actgtctttt
     3301 tgtttagagg aagacaaccg atccggtctg atttgttgct ttttcttttt actggacact
     3361 ttttccaact tttccgcttg ttttttcttt tcttggatcg atttttccaa tcgttttttc
     3421 tctttcatga tcgactctgc ttctcgtagc tgagtctttt tctccaattc cttttgagtg
     3481 agataggcac tataattccc cgaaaactct cggatttctc cgtcttttac ttcccagatt
     3541 ttcgatgcta gctcatctag aaaatagcga tcatgactaa caaaaaccag cgtaccataa
     3601 taataacgaa gctcttcaat cagtcgatca atactttttc tatctagatg ggttgttggc
     3661 tcgtctagaa gcatcccttg ttcataagaa gaaagaagac gtatcaactc gatctttcgt
     3721 tcttcccctc cgctccattt ccgctctgaa gtttcttcca gtcttaactg gaaatgactg
     3781 gctaattcca aatttaggtc ctttgcttca gcgagatagg ttaattgagg caggtagttg
     3841 aaggtgatct ctgtttggat tttcccatga tcgggaaaaa gttcaccggc tattagtttg
     3901 agcaaagtcg atttaccgac accattttct cctatgatcc ctatacgatc ttgctgatag
     3961 acacgaagag aatcaataga tagttctagc tggttgccaa agctgacttg tagatttgtt
     4021 atatttactg ctaaattttc catatgtttt acactcctat ttcatgtaaa acaacaatta
     4081 tcgatactaa aaaagagcag aaaaatctgt ttgcaaaaaa actgcatcac atcttttcct
     4141 actctgaacg aaataactat gactaaatca ccaaatctgt catagctttc acctattcta
     4201 ctgtcgttcg aaaaaatgga catactccat ccatgaaatt gttgtttata catgtctttt
     4261 gaatttattt agttgttatt caaaagttcg aaacgtaatt tcatcgatgc agtcattttt
     4321 ccgacctcct tttttattag gttaccacac acgattcaaa aaaagcaata gctactgaat
     4381 agtaactgaa aaccttttat tcgaacgtag tgatcttttc tggtgctaaa cgaacagact
     4441 cgtaaccttc ttctaccgct tttcctattg ataaaataat aactggaaca taacggtctt
     4501 tatccaaacc aaatgcctct gccaactgat ctgcttcgac tccaccaatt ggatttgttt
     4561 catagccatg atcacgcgta actaacatga attgcatagc cgctaagcta gaatcgactt
     4621 tgactacatc gttcatttct tctcttgaaa aactcttgta ataaggaatg atcgcagcta
     4681 attgttgatc tctgacttct tttggcatct tccctgattc gtaagcttgg ttatagattt
     4741 cttcgccata ttcgtaacat tgcatatcgc caaaaatcat gaccattgca gaagaagtat
     4801 cattttgtct cgtattgaaa cggatcaaag gacgcaagat ttcttttgct tcctcgcttt
     4861 cagcgaccac aaagcgccat ggttgcatat tgacagaaga aggtgcagtc gtcgcttctt
     4921 tgatcatttc cagcatttct tcgtgtggga ttttgtatgt ttcatcatat acgcgaacgg
     4981 atcttctacc agtcataatt gttgaaaaat catttttttg ttccattatt attaacctct
     5041 ttcgtttctg ttgttatatt gcaaagttgg gtattcaatt tatctaaaag tgttagaaat
     5101 tgtttcattt ctacatcgct gaaagccggg gatagcacgg aacctgtatc tttatggtcc
     5161 atttcacaat gctcgatttt cttctgggct ttttctgtta gcgctacata cacttcccgg
     5221 ttgttctgag gatttctctg acgcgaaaca tagccatttg cttccaatac cttcagatga
     5281 cgagtaactg ctccttgatc aatctgcaac ttttcttgca aactgctttg tagcaatgga
     5341 ttttcttctt tcagtctcat catgatctcg tatctagtaa gacttatacc gatattttct
     5401 tcaaagatct ggacgatttt ttgatcggct aatttgattt gatataaaag gtgactaatc
     5461 tcttctaaag acataatttc ctcctttcta ataattgact cgtcaacgat tgacttgtca
     5521 ataatagaat aaaaaaagaa aaaagtcaaa gaatcagctg tgtaataaaa aaaacgctct
     5581 caatagaaaa gtatagagaa aagaaatttt ttgtagaatg aacggaaaaa atgataaaga
     5641 aagcacaacg ctaaaaccaa gattattgat tcttatcaaa ctatagaaga tagctcttct
     5701 gcagtagaaa attttacacc gccaaaaatt tccgaaatcg ccttttgcaa gaatcccgtc
     5761 gaatattaga aattggatca gtatgcttaa acagtaataa aaaagttatg acaagatgtt
     5821 gtcacaacct tttttgagta aacggtgttc aaaaacttgc atctgcggta ataagttcaa
     5881 ccaaacaaaa aacatgaaaa tctgtttttg tttggctgtt cttactactt gatgcagggc
     5941 agctttttca ctacctcttt ttaataacca taaaatccta ttccttcata tctccagccc
     6001 atcggtgtta gccaatctct ttccttagca ctcaaggtat agtgatgggt accagttttt
     6061 tcatttggat taaatagtcg ataaattggc ttggaaccac cagaataaaa acttagacct
     6121 tcataacgcc aaccgatttt tgttaaagag ttgatctcag attgtgccat tgtgtaatga
     6181 tggtctccac tgtaaggatt gtataaacgg tagacaggtt gtccgctgtt tggtgcttgc
     6241 caagcaatcc cttcatattc ccagcctaca gttggcagat aatttttttc tccggcattc
     6301 aatgtatata aatgttctcc tgtatgacgg ttatacagac gatacatcgg agaagttgaa
     6361 gttggtgctg tatcatactg tgtcagatta taggtttcga ttacattgtt caatgtagaa
     6421 ccataattag gcgcagtggc ataacgacct gttagccaag ccgtagcatc tttgtaagag
     6481 ttcgtattgc ttttccatgc accggaatag tagtaaacgc ctggctgaaa agaagtcgtc
     6541 ttcaataccg ttacgttatc ttgcaatgat tctttgtacg aaggatattt tctaaaagcg
     6601 gcattcaccg taatccattg cccattcaca tattccgaag taggcatcgt aacagattgg
     6661 ccattatagc ttcctttaat cccaaataga ttgtaattag gggcagaagc caaagtactg
     6721 ttcccccagc cactttcaac gatggcttga gcaatcatga ctgatgcata taagtcattg
     6781 gcacttgcca aactttgtgc agttgaggca atactatcaa tgaacgcttg ttgagaatta
     6841 gcagatgagc gagcataaaa agcatagcca gtatagtttt cgaattcaga caaatctgct
     6901 tgatgggtat gtgtcaatgt ttgcccatct gtcaattggt tcgtgatttc ttctgcattc
     6961 tctggattgt tctgagcttc tgctgatgta ctgtcttcgg tttgtgtttg ttctgttgtt
     7021 tcagttgttg gttcttcggt tgaaattgta ttttctgaga cagtagctga ctgcaaagta
     7081 ctctctgtat cgttttcaat tcctgtttct gaagaactcg atgtctgagt tgcgacagtt
     7141 tctgtcgatt cttcagctag cactgttgtg ttggcaactg ctagatttcc taaaagaata
     7201 acgctaaata ataaattctt tgctttcatc ttttttctcc tttgaattat gaaacttttc
     7261 ctaaataaca gtgtaactaa aaaagtaaaa gcaagtcaat gtccatattt aaataacatt
     7321 tgtttctttt tgataaattt gctttttcta cataaaaaga cgtaaatgaa gaatattctt
     7381 ctccattcac gccttaaatt gatggtcgtt tgaataacta tttaacaagt aattctcttt
     7441 ggatattatc cgttatggat tatacttcct gtgtcgtcgg acggataatc atgttactga
     7501 cagacatccg gtctggcgtt tctatcgcaa atgctacagc agaagctata tcttcggagg
     7561 tcaatcccca ttctttttgc gcttcatgaa gttcaagagc agcctttttg tcgctgatgg
     7621 tttgatataa ttcagtttga acagctcctg gagagatgat cgtagatttg atgttatttt
     7681 gccgttgctc ttgacgtaat ccttccatga ttgcacggac agcaaatttt gttccgcaat
     7741 aaacggcgga atcaggataa acaacatgtc ccgccacaga atcagttgaa atgatatgcc
     7801 cagatttttg tttttccatg attggaagta cggcggaaat tccatttaac acacccatga
     7861 tgttgatatc aagcatattt tgccattcat cacggtgacc ttcaactaat ggagcggttg
     7921 gcatgattcc tgcattattg tacaatacat cgattcgacc gaacttttcc atcgctaaat
     7981 cgataactgc ttggacttgt gcaaaatctc ttacatctgc tgtttgaata tatagctcag
     8041 cttctggtaa agattctttt attgctttta ggcgatcttc tcggcgtgca gctataacta
     8101 gtttagcccc tttttctgct aatagcttcg ttgttgcttc accgattccg cttgaagcgc
     8161 ccatgatgac aatcactttt tctgataaat cattcattct attctcctcc tgttttgaaa
     8221 aatctcttgc ttatactcac tatctcttat ttcatcttac tgttcaaata atccgttcga
     8281 caggtttgac gattttggca gggattcctg caacgatggt attgtctggg acatctttcg
     8341 taacagttgc attcgctgca acaatggcat tctcgccaat cgttacgcct ggcagaatag
     8401 ttgctcctgc tccgatccaa gcatttttct tgatgtggat tggctttact aacaagcctc
     8461 ttcgttcttt tggatcggtt agatggttca ccgtgatgag gttgacacga ggaccaatca
     8521 atactgaatc ttctaaacag atgccaccta agtcaacgaa catcacttga cgattgataa
     8581 agacattttt ccctatacta atatgtttgc cgaaatctgt ataaaaaggc agcagaacag
     8641 tcaccgtatc atctactttg tcactaatga tattttctag ccgtttgcgg atatctgctg
     8701 gttcatggta cccagtattc agcgctgcta aaggctcttc gttttcagct tggatctgat
     8761 ggatcacttc aaagagcgga tccgttactc gaatctcttt attgacgatt tgtttttcga
     8821 tgaggcttgt cattttgctc cctcctttta tccttacttt ataacggacg gcttatcatg
     8881 tctaatactt ataaaacata cttaactatt ccttttaact atacttagtg gtatgctttt
     8941 ttaaaaacag gagggaaaaa atggatattc gtatactgac gtatttttta gcagtagcta
     9001 gagaaaaaaa tatcagccga gcagcaaaat ctctgcatat cacgcaacct actctctcaa
     9061 aacaactgaa agatttagaa gaagaacttg gtgtgacact tttcactcgg ggctcccgag
     9121 agatacagtt gacggattct ggtcgatatc tttattcaaa aggaaaagaa atcttgtcgt
     9181 tggtagaaaa aacggcgaac aatttaacaa atgacgcaat catctctgga gaaatctaca
     9241 ttggcggagg cgaaactaaa gcaatctctt tcatttctga tagccttcat tcgttgatca
     9301 aggcgcatcc tgatatccag tttcatttat atagcgggaa tgcagatgat atcatagaca
     9361 aactggatag cggattactt gattttggac tcgtaatcga accgacaaac aaacaaaaat
     9421 accagtcatt tcgactgcca gcccaagatc gttggggact tttgatacat cgaaatcatc
     9481 ctttagcaaa gcaaaccgtc atccatccgc aggaactgaa aactgttcct ttgatcattt
     9541 cccggcaaac gtcagtggac agtcaactag ctgaatggct aggtttcagc atagatcagt
     9601 tatcgatcgt cggaacatat aacttattgt acaatgcttc tttgatggtc gataacggga
     9661 cttttggtgc attagctcta gatgggatca tcaatactta tgggacggaa aaaatattca
     9721 tcccactcga tccgccatta caagcttcac tgaatctcat ttggaaaaaa gaccagcctc
     9781 tttctcctgc tgctcgtaaa tttttagatg tcctaacgga gaaatacagt taagtagagt
     9841 ttttttcctt cgctttcagc tttttaacaa cgaataagat aagcaaaaga tccaacgatt
     9901 aaataacata agtttcttaa tttatttctt ccttttattt ctgataattc cagtaaatta
     9961 ctataattat aaaaaaagaa ttgacaaaac ataactgtct atataaaatt gattacgtta
    10021 acaataattc gtttcatgtg actagatcat actaggcatg aaaaatctct gaggaacatg
    10081 gtttccctag gaggtttttc gtgtctattt ttgtttggcc gcaatcaact ggtctagcaa
    10141 acgtgaaaac caaatcttca aaaagggtgc gcgcatgaaa atcaaaaaga tctttaatca
    10201 aaatgcagta ttggtagatg acggtggaca ggaaaaagta gctattggaa aagggattgg
    10261 atttgacaga aaaagaaatg atttgatttt tgatcgtgat gtcgaacgga tatttgtcat
    10321 ggaaccagaa ggtcaaatca agttgcaaaa tttacttaat cagattgatg aacgcttttt
    10381 atttgcagca gaaacgatca ttgatcatgc ggaaactgtt ttgatggaaa agttgaatga
    10441 acatgtattg atcgctttga ccgaccacat tgctttttct gcggagaaca ttaataacgg
    10501 tatcgtcatc cgcaacaaac ttttaagaga aatcgaagtg ctttacagtg atgaattcag
    10561 tattgctcaa tgggcagtag agtatctgac aaaagagctt gggattccat atacttatga
    10621 cgaagcagga tatatcgcaa tccatattca cagcggacga agcggtcgga acaataacca
    10681 tcgcagtatc cgtgaagtga cgattgtatc tgacatcatc catttagtgg aatcagaatt
    10741 ggaaacagat atccattcag aaccattttc actgaattat tcaagactgg tgaatcattt
    10801 acgactgctt ctacaacgaa cccatgcgca acaatatgct gttttagaca cggaaatcgt
    10861 agaaatggtc aaacgaaaat acccagaaag ctataagatt tctaaaaaaa tccgtgtgtt
    10921 gctgaccaaa gaataccagc tcgcgatcac gaaagaagaa ctgggttact tggccatcca
    10981 tatcgagcgt ttaagaagcg caatcgtaca aacaaatgtt aacaatcatg aggaggaaaa
    11041 aaattgaaag catatctaca acgaatggga cgttcccttc aacttccagt tgcggtctta
    11101 cctgctgctg ctttactagt agggatcgga aactggtggg catcttatag taacgacatt
    11161 gtcgctcatt ttttacaagc tggcggtaac gcagttttag gtcaattacc tttgctgttt
    11221 gccgttgggt tagcactagg gatgtcagtg gacaaatcag gtgctgctgc cttggcaggt
    11281 ttggtcgcat ttgaagttcc agtaaatgtt ttaaaaactg attcagtagc tactttattg
    11341 aatatcaaac cagaatcagt tgatccatct tttgctcaga tcagtaacgt atttattggt
    11401 attattgccg gattgattgc ggcagctctt tataatcgtt tccacgaagc aaaattaccg
    11461 atggctttat ctttctttag cggcaagcgt ctagtgccaa tcttggctgc ctttgtcatg
    11521 ttgctgatca gtgcagtgct actgctcgta tggcctccgg tttactctgc tttagtttct
    11581 tttgctaaat tcattttgag tttaggctgg ggtggtgcag gtctttatgg cttcttcaac
    11641 cgtttactga tccctacagg attgcatcaa gcaatgaact ccgtgttctg gtttgacgta
    11701 gcaggaatca atgatattgg taaatttttg gcttctgaag gaacaaaagg cgtaacagga
    11761 atgtatcaag ctggattttt cccaatcatg atgttcgggc ttccagcagg agcatatgct
    11821 atttacagaa atgcacgtcc agaaaacaaa gcaaaaacag cttccttgat gctagcagca
    11881 gcttttgctt cattctttac tggtgtgaca gaaccacttg aattttcatt tatgttcgtt
    11941 gcttggccat tatatgtctt gcatgcaatt ttcactggga tttctttagc agtgagtgca
    12001 ttcttccatt ggacagcagg atttgccttt agtgccggat ttgtcgatta cttcctatct
    12061 ttgaaaaatc cgattgcgaa tcagccattg atgttgatcg tccaaggatt agtaacagca
    12121 gtgatttact actttggctt taactttgct atcaaaaaat tccatctgat gactcctggc
    12181 cgcgaggaag cagatctttc agatgacgat actgcaacga ctaattcaga caataaatat
    12241 gcggcacaag caagcaaaat ctacgctgca ttaggcggag ccgacaatgt aacttcgatc
    12301 gataactgta ctacacgcct tcgtttgcaa gtcaaagata caggcctgat cgatcaaaat
    12361 aaaatcaaag ccactggcgt acctggtatg aagatcatcg acggaaaaaa tgcgcaagtc
    12421 atcgtaggta cagaagtaca attcgttgca gatgagatgg caaaactaca tggtggtgct
    12481 gcagcacgac ctgcacaaac aaacaccgtg gttaaaacag aaacatttgc cggtgaaacg
    12541 atcgaacaag atatttacgc tatcgcaaat gggaaactga ttccaatcac tgaagtttca
    12601 gatgatgtat tcgcggaaaa aatgatgggt gacggctatg ctgtattacc agaaaatgga
    12661 gaaatcttct ctccaatcgc tggtacgatc acaaacattt tcccaacgaa acatgctgtc
    12721 ggaatccaaa cagatgctgg tatcgaagtc ttgcttcata tgggcatcaa cactgttgac
    12781 ttaaaaggcg aaccattcac tttatatgta gaagaaggtc aaaaagtcgc aagaggtcaa
    12841 ttgattgctt tagttgatct tgccgctatt caatctgcag gcaaaaacac ggatatggtc
    12901 gttgtattca caaatggcga caaagtccaa agcttagaaa tcgaacccgc acgtgatgta
    12961 aaagcaaatg ataaaatcgg ctctgtttcc aacaaagcct gagccatcgt cttccccaga
    13021 cttttttatc gataaacaaa caggagctga gacaaaaatc ttgtctcagc tcctttttcc
    13081 gaataaaaac ggcgttctcc tgccaactcc gcggtaataa gtccagctaa acgaaacata
    13141 tgaagagcta ttttttattt gactcttctt attacttgat gcagaacagc tttttcacaa
    13201 aatttttctt catactcaaa actaatcggc tttttcacaa cctctttata cattattcct
    13261 gatccaatat ttcttttaat gctgcttcaa gagaagttaa tggttttccc aatacttttt
    13321 ctaaatcatc agaggaaata gctaactgat cctttttaat atcatgttgg atcgctaaaa
    13381 acatgtcagc tgcttcttga gggaatccgg cttcttttag attttcgaca aacgctgcat
    13441 cacttgcttc aataatttct aactctttac ctgtcgcacg ctctaatgcc ttggctaatt
    13501 cttcatagtc agttagattg ccggataatt ctaagatttc tggagagtca ctttctacta
    13561 gtacttttgc tgcagcttct gcatattcac gtttcaatgc ccaacctgtt tttccatttc
    13621 ctgcagcata aacgaattga ccagttttta aagcctgccc aatgataggt aattcattct
    13681 ctacatacca attattccgt aaaaatgtat gggaaatacc tgaatcctta atcatttttt
    13741 cagtgaattg gtgatctggc gctaatacac tgatcgagtg atcggcatct gcaaaacttg
    13801 tataagcgat ataagatacc ccagctgctt gtgctgcctt cactacattc ttatgttcgg
    13861 cttgacgatt tcctggaacg ccagagacaa aaagcaatcg atctattcct ttcaacgctt
    13921 ctttcattga gtctaaatca tcataatcac caatacgtat attaaatcca gcttctttca
    13981 gtgcttctcc tttttcttca ctacgtacta gtacatagat ttcattttgt ggaaccatct
    14041 tttttaagaa ttccagcgca taaccaccaa aacctccagt tgctcctgtt actaaataat
    14101 gcatatttgc cactccctat tctttttatt tgttaagatt tacagctgta catttataaa
    14161 ttacaccttt atcataaaga gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga
    14221 caactgatga agtattctct gcaatttagt gatgcaatcc atgtacttgc ctatattgaa
    14281 atgtttcaag atacccgatc actttctagt gagatgattg ccagtagtat cgaaacaaat
    14341 gcagcaaatg tacgtaaaat catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa
    14401 accggaaaac cgcagccttc ccttgccaaa agtccagatc agatttcttt actagatgtc
    14461 tatcgcagca tcgaaggtaa tacacagctt atccaagtcg atccaaaaac aaatccaaat
    14521 tgcgtcgttg gtgcgaatat ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa
    14581 aaggcagaag aagaaatggc taagatcaca cttgatagtc tgatacataa aatagccgtt
    14641 ttggaaaaag aaaaaagacc agaaaacaca ggcctcgtag agagattttt ataattctct
    14701 ctactgtgcc tgcatttttc tgatcggaat cgtctacata aattctttta ttctccgggg
    14761 gacaaaagcc ttgtcatgcc cctttttcct ctctaatgct caggtcaaag cgctgaagtc
    14821 atgacgtctt gataaacggt gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc
    14881 aaaatatgga aagctatttc ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt
    14941 cacgacctct atattatcat ggtttattga ctaccggtat ccagatttct cccatatatt
    15001 tcccgttggt aactcccatt tcgactacag catttggacc cccaacatag gcgtaatctt
    15061 ctacttctct taaaacatgc ccaaaggccg tattcgtcaa atcatttctt aattcttcag
    15121 gcgattctgc taaagcgctt acaacaagat acgttccttt tgggaatggg atgattcgcg
    15181 tagtgtttgg aacatcctga ctcgtttgaa caccgacata gtacatcatt ttcccgttat
    15241 agggttcgtt gactgtaaag aaataatgat tttcagcagc ttgtttcaat gcatctattg
    15301 ttccattacc gataactttt tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat
    15361 ccgtattgct actcttcaat tccacaccaa tacccgtcac cgtaaaatct ggtttttctt
    15421 cgatcctata ctttgccatt tctcccaccc tttctattat attaagttca tcttacataa
    15481 aattaaaggg aaatgacacg attacgctct tttttctagg gatattttta ttttttaact
    15541 atttttttgc tttcactgca atccaaatct cactgtaaac attactgaaa tcctctcccc
    15601 attttgtaaa agagatctcg ggagcttcga ctaacccata atctgaagaa ggaagccatt
    15661 ctgcatagat tttcccccaa gtatcttgta aagtttgggg aaatggccct ttattaggaa
    15721 aaatggccca agtgtgttct tctatagcaa gcatttctaa atcagcgtac ggattttctt
    15781 ttgtcgtagc aaagccaatc atgtgcatca aactgccctt ttcttccaag cggtctccat
    15841 caaatccata agaaacgttg attacttgat ttggatataa atctcctaac ttatgcatct
    15901 cttccctttg agtctccgtg atcgtctgcg ctaacttttg tatttcttga ttgactcctt
    15961 caaatttgac aggaactttt ttcgccacac ctgccacata aaactgttct ttcttttcga
    16021 tacgaaattc catcgatttt cctcctctga catcaataaa aaatgtcaag cgtggaaaag
    16081 atttttgtat gccgtatctg ctgatttctg aaggcagata tcctgaccaa tcacgaaaag
    16141 ctcttgaaaa accttcgact gtttcatatc catatctaaa cgccgtttct gtcactcttg
    16201 atccatctag aagatcgcga ttagccaaag ccaatctacg gtttttgata tagtccatta
    16261 gcgacatccc tgcaataaac gtaaagatgc gttttaattg atattcagag attcctgtat
    16321 tttcggctat tattttatag gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca
    16381 attggttcag atctttaagc atatttatct tcctccttga ttgccttttt atcatattat
    16441 caaagaaagt caattacttg atgaatcagg aaccaattta gtcagatgta gaaaatgttg
    16501 tatggatcta aagaaaattc tcaagcagcg tttcgtaacg gataattggt attatctgcc
    16561 tatctatgtt ttacctttag aaaaataaat tttggggaag acagattcta agtaaacaaa
    16621 gcggtaaaaa tatcttttat tcccttagtt aatccgctta ctacctactg tacaaagcaa
    16681 attgttgttc aaactgctga tcagaacgct gcttggattt ttcttt
//
LOCUS       DACJGB010000067        16697 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.068, whole genome shotgun sequence.
ACCESSION   DACJGB010000067 DACJGB010000000
VERSION     DACJGB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.068"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..415)
                     /locus_tag="IYI34_002263"
     CDS             complement(<1..415)
                     /locus_tag="IYI34_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAR0560108.1"
                     /translation="MYVEKLYEMILIVKYRVEVLFMVTRIFILDDSLVFEKMLVEILE
                     ESRILLSPWKFEIVQGLNVIQFVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLRE
                     KNEKCKIVYLTSHPNKAITVINRKITPSGYLIKEFT"
     gene            complement(600..1436)
                     /locus_tag="IYI34_002264"
     CDS             complement(600..1436)
                     /locus_tag="IYI34_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAR0560109.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1447..2376)
                     /locus_tag="IYI34_002265"
     CDS             complement(1447..2376)
                     /locus_tag="IYI34_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAR0560110.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2568..2921
                     /locus_tag="IYI34_002266"
     CDS             2568..2921
                     /locus_tag="IYI34_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAR0560111.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(2995..3651)
                     /locus_tag="IYI34_002267"
     CDS             complement(2995..3651)
                     /locus_tag="IYI34_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAR0560112.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            3877..5811
                     /locus_tag="IYI34_002268"
     CDS             3877..5811
                     /locus_tag="IYI34_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR0560113.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(5897..7120)
                     /locus_tag="IYI34_002269"
     CDS             complement(5897..7120)
                     /locus_tag="IYI34_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAR0560114.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7114..9012)
                     /locus_tag="IYI34_002270"
     CDS             complement(7114..9012)
                     /locus_tag="IYI34_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAR0560115.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9006..10124)
                     /locus_tag="IYI34_002271"
     CDS             complement(9006..10124)
                     /locus_tag="IYI34_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAR0560116.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10207..11040)
                     /locus_tag="IYI34_002272"
     CDS             complement(10207..11040)
                     /locus_tag="IYI34_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0560117.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11033..11950)
                     /locus_tag="IYI34_002273"
     CDS             complement(11033..11950)
                     /locus_tag="IYI34_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAR0560118.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12177..13301)
                     /locus_tag="IYI34_002274"
     CDS             complement(12177..13301)
                     /locus_tag="IYI34_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR0560119.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13484..14392
                     /locus_tag="IYI34_002275"
     CDS             13484..14392
                     /locus_tag="IYI34_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560120.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14533..16146
                     /locus_tag="IYI34_002276"
     CDS             14533..16146
                     /locus_tag="IYI34_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAR0560121.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5287 a         3260 c         2765 g         5385 t
ORIGIN      
        1 aagtgaattc ttttatcaaa taaccactag gcgtaatctt tcgattaata acagttattg
       61 ctttattcgg atgagacgtt agataaacaa ttttacactt ttcattcttt tctctaagca
      121 tttcgcctag ttcgataccg ctataatagg cgttcaaatc gatgtctagg aaaaaaatat
      181 ctgaggaata gatttcatta tttttaatga attcaacaaa ttgtataacg ttcaatccct
      241 gaacgatctc aaatttccaa ggacttaata aaattctgga ttcttccaaa atttctacta
      301 gcattttttc aaaaaccaaa ctatcatcca aaataaatat acgcgttacc ataaaaagta
      361 cctccactcg atattttact atcaaaatca tttcatacaa cttttcaaca tacatgttag
      421 gaataaattg taattctctt cacaatcttt tccaacacct ttgaaaccga tatcaaatac
      481 tttcaggatg ctgcttctaa aataaatatc aaacaaatga atacatcatg caaaaaaagc
      541 cagacaaccg acgtccttgc gtgtcagctg tctgagctat gtttatttat tttgttcttt
      601 taaaaagaac gatacacata ctgatttttc ggagcagaga ctttctcagc gtttgtaaca
      661 actacagatg ggatttcacg cttgaatggt tgataatagt ctgattggat ggttccttct
      721 attttatacc attcatcatt tttaaccgtt actccttccg gcatatgcgt caacaaacca
      781 aagactcctg aatctgccac acaatggata atgccgaatc ggaacaagaa gatatctgta
      841 ttgctatcgt tcggtgtttg gtaaacaaat ccttcatagc tgattttttt gccaatgaat
      901 tcgctagggt aattataaat tagctccata acttctaaat agttttcatc tgtgatcttg
      961 atagtttgct tatccccata cttttctttc aactcattca tttggtctaa gtagtctgat
     1021 ttgttgaaat aaatactggt atccggcttt aagtattgtg tctgcatctc cggatcccct
     1081 actgattctt tacttactgg gaaattaaat cctttggctt ctacgatcgt agtgtcgaga
     1141 ctaactgttg gaaacaacgt ccccacgatt actggcaaag ctagcagtac ataagcgatt
     1201 ccacgttgat aaggttttga caacccatgg tcatgatcgt gctcgtgtgc cttttcttct
     1261 gcttttccgt ttttgaccca caaatacagc tgaactaaag ccaaaagagc agataaaatc
     1321 attgaaagaa tagctaagta gcgataatgg acattgatat attgatcgag tttgccagaa
     1381 acttgcaagt acatcatcaa ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt
     1441 tccctcctaa ataaataatg tgaatccaat aacgctaagg gcaatcagtc cgatcagcga
     1501 taaaataaag cgtcctgtaa aatatcgttt catcatcagt agatttttga tatcgaccat
     1561 tgggccaaag accaaaaagg cagctacagg tgctgtgccg aacagactta acaaagaaga
     1621 tcctataaac gcatctgctt ctgagcaaag agacatcgta aaggccaata acagcatgat
     1681 gataatggct aaaatctttg ttgagccgag cgttagcata attctagttg gtaaataagt
     1741 ctgcatcgca gctgcgatta atccgccaat gatcaaataa cgacctgtat caaaaaattc
     1801 gtcgattgaa tgtgtcaaca catgcccagt ttgatggatc agctgatttt ttggttcatt
     1861 cttctcactt tgttcttctt catggacatg atgatggtga ttttctgcta ctttagacgt
     1921 gattttttct ttcaatacag cgtctttttg aaaataggca agccagacac caatcacaag
     1981 ggcaacaaaa aagctgccta tcacccgcca gcttgccatc tgccaagaat tgccaaatgc
     2041 gataaaagtg gaaaaaatca cgattggatt gatgatcgga gcagtcagca taaaagcaaa
     2101 cgctgtgtga actggtacgc cttttttaac aaattgatga acgatcggta cgatgccgca
     2161 ttcacatgaa ggaaagaaaa atcctaagat cgaaccagta aaaatagaga agatacgatt
     2221 ttcaggaagc catctcttca cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat
     2281 aagacagcct aataatacga aaggtaatgc ttcaataacg atcgataaga aaatcgtact
     2341 catctgcaaa accgaatttg gaataaaaga aaacatgagt ttcctccttt ttatttctag
     2401 ttcattcgct ttgcaaatat acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt
     2461 tacacaactc ttatttttta ccaaagcact tcaattgact ttttctgtta ttcccattaa
     2521 attaaggtct catacatatc acttctatca taaaagggag aataaaaatg aaacaaacca
     2581 ctaacttgac tgaagttcaa gacatactgc aacagtcatc cgttgccgtc atttatcttt
     2641 cgatgcctaa ttgcagtgtt tgtcacgctg ttcgtccacg actagaggaa cttctcacac
     2701 attatgatat tccggcactt catttggatg catttgaaac accagaagta gctagtaaat
     2761 ttgaagtgct gactgcgcca gttgtcctga tttttcatca aggaaaagaa gtctttcggc
     2821 aagcacggtt catcgacttt aaaaaaatcc gttatctact agatgaactg acagcagaag
     2881 atgattccct ttcttatgaa gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa
     2941 aagcgattga acaagtgaaa gttttgcctt catttgttca atcgcttttt atttttattc
     3001 tgctgtattt tcggaatgcg catttgcgtc agaatgcaag aaatagatca aggtttgcaa
     3061 ttcattcgtc aaatcaacat tttggatcaa tacatctgac ggagcattca atcgagcagc
     3121 ggtaaagttc aagattcctt tgattcctgc ttccgttaac tgattgacta cttcttgtgc
     3181 tctttcagcg ggtatcgtta aaattgctac ttcgatttgt tggatcttga tttgttctga
     3241 catatcttcc attggataaa cggggatacc gtctacgatg cgcccaacga tttcctcatt
     3301 cacatcaaaa gcacaactga tacgtatgct attactttga tggaatttgt attttagtaa
     3361 agcacttccc aagttcccta caccgatcag cgcaacattt gtcaattgat cgtcgtttaa
     3421 tgtctttgca aaaaagtgca taagatcttc tacatcataa ccatacccac gttttcctaa
     3481 ttcgccaaaa taagaaaaat ctcgacggat cgttgcacta tctacttgaa cggcttcact
     3541 taattcagtt gaagacactt ttttctttcc agcattattt aaaatcctta aatagcgata
     3601 atacaaaggc agtcttttag ctgtcgcctt tggaataata tgatctttca cgaaatgacc
     3661 tccacgtatt gtctaattac atctttgatt tctccatcat agcagtattt ataggaaaaa
     3721 atcaattcca ttgctcagat attgtgaaat tttattcttt attcatttat ttatcatttc
     3781 tgcaaatctc gcagtataat ggaatttatg gtagactact aattgaaaaa tagtttttta
     3841 tttgaggaga attcacaaag tagaaaagag gattccatga tattattaca agccaatcag
     3901 attgctcgcc attttggtgc agaagtctta tttgaaaaca tacatttaga aatcagtaca
     3961 ggcgctcgtg tcgctttagt tggcagaaat ggcgctggaa aatcgacttt attaaagatc
     4021 attgctggaa ttgaagcacc tgatgaagga aagatcgcta aaaataaaac agcgactctc
     4081 ggatacctcg ctcaggacac aggacttgat tccaatgaaa cagtttggaa cgagatgctg
     4141 aaagcttttg aacaagttag acaaatggaa gagcggatgc gcgagctaga gattttcatt
     4201 agcgaaggta cgccagacac acctgcttat agttcttatt taaaagaata cgaccggctg
     4261 caacacgatt tttcagatcg aaacggctat ggttatgaaa atgaaattcg ttccgtattg
     4321 catgggttta aattcgattc ttcattttat gatcagaaaa tcgatacttt atcaggcgga
     4381 cagcgcacac gtttagcttt agctcgcatg cttttgcaac accctgacat tttgattcta
     4441 gatgaaccaa cgaaccattt agatatcgag acactttcct ggcttgagaa ttatctgcaa
     4501 agctatactg gtgcattatt gattgtttcc catgaccgtt attttttaga taaagtagtc
     4561 aatgaagtct atgaattaag cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa
     4621 tatctagatt tgaaagcaga acagttagca agtgaatgga aggcttatga aaagcagcaa
     4681 acagaaatcc ataaattaga agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca
     4741 aaacgagcgc aaagccgcag aaagacatta gaaaaaatgg accggctgga tcgtcctcaa
     4801 ggggatgaaa aacaagcaaa ctttctattc agtatagaaa agccttctgg aaatgtagtc
     4861 ttgcaaatcg aagatgccgc tatcggatat gataatgtca ccttatctga accaatcaat
     4921 ctagatatcc gtcgtcaaga agcgattgct ctagtaggtc cgaatggtat cgggaaatcg
     4981 acgttgctga aatcaatcat tcaccagatc ccttttatcc gtggaaaaga aacgctaggg
     5041 acaaatgtca ccatcggcta ttacgatcaa ggtcaagctg atctgcatac aaacaagacg
     5101 atcctagcag aattgtggga tgagcatcct accactccag aaaaagagat ccgaagtgct
     5161 ttaggtgctt tcttattttc tggcgaagat gtagaaaaac caatccctct gctaagtggc
     5221 ggtgaaaaag cacgggttgc tttagccaag ttagcgatga acaaagaaaa ttttttgatt
     5281 ttggatgaac caacaaacca tttagatatt gacaacaaag aagtattaga aaatgcattg
     5341 atcgattatg acggcacact gctttttgtt tcccatgacc gatatttcat caatcggatc
     5401 gcgacaaaag tcgtcgaact ctctgaaaat ggcagcaaag tctatctagg agactatgat
     5461 tattatttag agaaaaagca ggaagaagaa gaaatcgccg aattgttggc acaagatgag
     5521 ccagaatctg ctcctgctcc cacgaacaaa tctgattatt accaaagcaa agaacagcaa
     5581 aagcttatcc gtagccttca acgaaaaatt actcaggttg aagaagaaat ggcgcggatc
     5641 gatgaatgga tcgatcagtt ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac
     5701 gtaaaactca atgagctgaa ccaagattta gaagctgcta gacaagatca agaaacaaaa
     5761 ttgacggaat gggaagaact aagcttggaa ttagaagaat atgaaaatta acgaaaaaac
     5821 caaaacagaa accattcatg ctgttttggt ttttcttttt ttattttatc aaacaaaaat
     5881 tccatattta cctttttcac caataagaag accaattact agtcattctc actaaagctt
     5941 ctaaataata gtaatcaccc caaatacaag gtgtatttac cccattattt ttgggcttat
     6001 tataaacaga ctgtgttaaa aagccatctt cttcttccgt tgtagcatat ctttctttca
     6061 attgtcgcat aattttccaa acagcagact ggtataagtt cttttctgtt ttgtctaata
     6121 gacctgattc tattaattct aataatccac aaaccatgat caccatacta gatgtatctt
     6181 tttcttctct gctagaatca tcaaagatca aatcccaata acaaatggta tcttctggca
     6241 accgattcat aaaataattg gctaatattt tgcttaaatc aaaatatttt tgttctttca
     6301 aataacgata acttaatgca aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg
     6361 aattatcact ataaccttga tgcgtacttc catatcttgg ttttccatct ttgacatcca
     6421 tataaaatgt atgataagtt gaaccatctt ctcttactaa ataatctgct gcattttgaa
     6481 tatgagcctg agcagctttt ttatattttt gatttccagt ttcttcactc gcccaataca
     6541 ataatggaag attcatgcaa caatccatga tcattctacc ttgttgtgaa ggatcactag
     6601 gatctcccca agcttgtata actcctgcct tctcccaaaa acgctgaagt aatgtttctg
     6661 cagcatcaat tgaagcttcc ttcatttttc gatttcccgt tattttataa tctgctacgg
     6721 ttgataaaga atacaaaaaa ccaagatcgt gatgattagt gataatttga cagtcaatcc
     6781 tgtggataaa atctatatct gctttttcag ctgtttgttg aaataaagga tcacccgtct
     6841 tctcaaataa caaccaatta attcctgtcc aaaatccttc tgtccaacca atattgtctc
     6901 ttaatggata acgtcctttt ataggacact cgcttgggaa ttgatactga ctgttactga
     6961 tacgtttgat taacctctcc gtgtgtctgc gacaacgctc gatctcatta ttccattctt
     7021 tttccattaa ttctttataa cattcattta tccatttttc tggatgtatt atctcttcaa
     7081 acatcacttt atcaagcatt tccccaccag cctttagcat aattttagtc tttttttatt
     7141 gtttttattt tggatagtta gcccggttgt cccatcaaaa tcaattttgg gacgattgtt
     7201 ccatacttct aaactataat cccgatcttg atgagcaacc actcctgttc cgagccaatg
     7261 ggttcctttt ccaaatgtac cctctaatcc aggaataaaa gtgttttccc atgtcatcaa
     7321 gtttgtattg ggcaaaccca taatagctgt ggattttcgt ttaatagtat cgtcacttaa
     7381 acagataata cctgaaaagc cagcttcact caccctatag ttttccttgt cttcttctat
     7441 gattattcct tctttctcag gagaacattc aatagcaaac cctgtctcat atagaacata
     7501 ttctgtatct aattctactt tgtgtacgcg aatctgccag ccttcaaaag gaataagcca
     7561 agttctgatt tttgcctgta aagtaggttc ccatgtagag gcaagtacct ctttcttaca
     7621 tacaatgtct cgtacattat ttcgaacgat gaactgtcca taggttttga tgctctcatt
     7681 cttaaaactg gtcgtgatat atgtgtccgc tcgctgaatc cctagcattg aatccattgc
     7741 catttctgct ttcaattgat tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc
     7801 aaattttaaa tatttttcat tggtcaatgt atggaaattt ggccctaatt gtcccgcatt
     7861 caaaaaaaat gtatgtccat tatcatgttg tatgagcaaa ttggcttctg aaagaagttt
     7921 agttccttca cgaataggca taggcatttc tttagccgtc caatactcat ggtcttcagg
     7981 tagcgccaac aataaaaaaa tcttattcag ccataatgga gataacgtac tattataggg
     8041 ttcagtcaat gtcaattgag ggtaggtata accaatattc aggatacctt tttcatcaaa
     8101 tatttctttt tgcatccacc atcttatatt ccgattgact aaccccttga tttcacctaa
     8161 tttaaaaggc aaaagatcgg cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg
     8221 ataaatcatg cttcgaccaa aaggcacatt tgcaccatct tccgcaaaaa aatgaatata
     8281 gtctctagaa aaatcaatag ctcggttctt atatatctgg ctaatgggct tttctggttc
     8341 tagtacgcta taaatcaaac cataataatg aaatgcaaaa ggattataat aatcttggcg
     8401 acctcttgaa ctgtctcgat accagccatt ttgaagataa caattttcta tattatcaaa
     8461 tgcttctttc agtgctgatt ctttaactgg ataaccaaat ttttggataa ttgttccgac
     8521 aattatctta aaaaattgcc aattaccatc tgcacattca tattcaaaaa tttgcgagaa
     8581 ccaatgaata atctggcttt tttctcctgt atctaatttt ttccataata attgttcatg
     8641 atgatataac gctaataaca ttgcgggcat ttctaccata cgctggtctc tttcatgtat
     8701 ttctccccaa taatcgggat gggaaggatc tgttccagct tttattcctg atagaattaa
     8761 tttcaaccat cgttcatcta attggtcaat tgcgggcccc gctccccata aaacccgaga
     8821 gaatccttcc attcctgcaa ttttatttcc atatcctacc ccttgatgta tataatcaat
     8881 ttgtgcgcca cttgctcgat aaaacggctg gagtggttga tttatctttt taaataagtc
     8941 aaagtaatcc tgtctggtcc ttaatagtgg tactggatgt ttcattgtat tttctcctct
     9001 ttttactacc aaatagctaa ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa
     9061 taatcagaat agatcagtgg cacattataa gtcagttctc tatggtatgc ttctgctccg
     9121 cctccaatga tcccatcttc atccatattc caatttgcat agtgcttttc tgtcttttgg
     9181 agtatattga tagcagcttc atgatacatt tttgatcgta cagaggaaac atatcctgct
     9241 aattccagaa gaccgcatgc agcgcatact ccagcactgg tatcaatatt cgaatctttt
     9301 acagcaaaat caatgagagg aatataacca gttttttcga ttttctgaat aaaatttact
     9361 gctatctttt ctgctatagc tagatagtac ggattttttg tgtaacgata acttaacgcg
     9421 aatccatata aagcccatgc atttcctctg ctccaacaag aattctcatt gtatccttgc
     9481 ccgccaagta accgaatata tttccctgat tctgcatcaa attctgcgat atgcccggtt
     9541 gatccatctt tcctcactag atacttagct gttgtatctg catgctgccg agcaatttta
     9601 gagaacctcg gatcaccagt ttccctcgaa gcccaataca gtaatggtaa attcatcata
     9661 ctatcaataa tcatccaacc aggtttatgg tcattccatg ctactaaata gttccctgaa
     9721 atattatagc gtccagcaag taccgtagct gcatgtaacc ctagttgtcg actatgtttg
     9781 ttttgagact gttgataatg tgcaatagcc gatggtaaga ataaaaaacc tacatcatga
     9841 tgaacgtctg aaaaatggta cagggcagat tctaataatt gttcttgttg tattgctgcc
     9901 tctttgaact tttctcgttt tgtcttgtga aagagctgcc ataaaatacc actccaaaaa
     9961 ccattggtcc accagtcgat tcctctgtcc tttattacgt ctgtatattt tccgttgtaa
    10021 gaaatatatg gaattttatt ccccattctg tctaattccg catctatctt ttcagaagct
    10081 acattcaaaa tttttcttga ccattccttc gtctgttcat tcatggatat cccgccattc
    10141 ttttaatttt tatgaatgtg gagttgctcc tagaacgaac tagatggtta acattcgtta
    10201 ttttttttac ttatttgcag ctccaatgtc attctcctta aatttagact gcgcccacac
    10261 ataaatcaag accattggga tcactgaagt tgctaaaatt agcataattg cgccataatt
    10321 aattggctca cctgtactag aaaccgtcat atttttgata attaagggaa ccgtatattt
    10381 atctttatct gaaagcatga ctaatggaaa tagatattga ttccaacttc ccataaagat
    10441 aagtaaacca ctagtaataa tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat
    10501 cacccactca ttggcaccat cgattctggc agcctccatc agtgctgtag gaaattgact
    10561 gctatattgt cgcatcataa acactgaagt tgtactggca atcgaaggga tgatcacacc
    10621 tgcataagta ttcgataatc ctaaacgact cataatgtaa aataaaggaa tcaatgtggt
    10681 aaaaaaaggc accatcctag atagcataac tgtattgaac aacagtttat tccccataaa
    10741 acgatacttt gctaatgcat aacccgccat tgtagaactg gctgtagcta aaactgtact
    10801 gattacagca ataacaatcg aattccaaat aatcaaggga taattatatc tcgccatgat
    10861 ggcttgccag ttttttaata gcatatcccc aaacttgaaa ttgattccgc cacttaaaat
    10921 ttcttgatta ctttgcgttg aagctacaat catatataaa tatggcatca aaaatattaa
    10981 ggcaccaaac acaataatga cgtacgtaaa aattctgctt tgtatctttt gtttagtcat
    11041 ttttatcacc tccgaattta aattgaatag ccgataatat aacagataca aacaccatcg
    11101 tccaggcaat agcagaggct tttccaaaat caaccatttc aaaacttgtt ttatataaat
    11161 tgactcctac gaccaaagct gaattttctg gtccattgga agtcccaaag aatatatttg
    11221 ggatattgaa attacccaag ccattgatag tggctaaaac tgtggccatt aaaatgattg
    11281 gctttagcat ggggatcgtt aataaaaaga attgccgaaa tttcgatgct ccatctattt
    11341 cagcactttc gtatatctct ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt
    11401 tatatcctac actaagccaa atcatcgtga tgatcattga tattcgtatc caaaaaggat
    11461 ttgttaacca ctgtacaccg ttaattccta gcgcattcaa aaatccattg aataacccag
    11521 tcggattgaa aaaagtcatg aatacggaag ctacagccac cgttgatgta atggttggaa
    11581 tataatagac agttctaaaa aatccttttc cttttaactg tgctgaattc aataaaacag
    11641 caaagtaaat agctaatagc atcgttattg gaattgatcc tgccatgata atcaaaacat
    11701 tcgtgtaaga tttaataaac aatggatctc ggaagatagc tagataattg tcaaatccaa
    11761 caaattcaaa tgagtagcca attttcataa aactaaaagc aaacgagata atgatgggaa
    11821 tcaaaatcgt cacaataaag aaaataacaa aaggtgtgac caatccataa gcactttttt
    11881 tatgagacgt atctgttgct tttttcttgc tgttctttat tgtcttgctt ggtaacgaag
    11941 attgcatcat cgttaagtcc tcctaatatt ttttaatcac cagttaattt tacgccgtac
    12001 ttatgagcaa aattttctgc ttcttttaga tatgaatttt caaaatcacc tgtttgccaa
    12061 tagttatatg cagcggtagt caaataagac gtagcatctg aataagtggc tggtaaaaca
    12121 gtatttgatg tattttcatt gattttattc aatagttcat agtatttttg tcctccttag
    12181 tagtcaaact ttttctcagc tgcttctgtt tgataggcca atgagttaga tgtagccacg
    12241 cccagctcca gtgcgttggc taagcattct tcatctgtca gcataaatgt aagaaattgt
    12301 tgtgccactt gtggattttt tgcatcttta gggacataaa aacttgatcc tcctgatact
    12361 gcaccttttc ctggattttc ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca
    12421 gatgcatcag gaaaattcaa agacatattc gtagcatacc agccgccagc aacaaacatt
    12481 gcagattctt gaaatgcttt ctcagcatct tgtgcaccgt agaaatgtac aatatctgag
    12541 tcgatcattt ctttgattaa ctttgcagca tgtttcgctt ctttagtatt cagacggata
    12601 tttccttctt catcaagcaa agattgattc tgctgggcta gcatgttgat aaacaattct
    12661 tggtccccag attctggcaa ggcaattcca aaactaccag ttttttcttt taatgctttt
    12721 gccacctgga tatattcttc ccaagaactg atttcttcaa aatctacacc tatttcagca
    12781 aatttttttg gttgataata acaaagtgct ggggtaaaat cactagccca aggaataatc
    12841 gtttcattac tcgtttgttg acgcaaaata ttcaattttt gatcataaaa ctgatcgcca
    12901 tatttgtcat aaaatccata ttcatcagca ctataaaaag catcttgaaa tttttccagc
    12961 acacctgctg ctgaagtgtc tgccatggag ataatatccg gcatatcttc gtgtgttacc
    13021 aattttgggg tgatcatttg tactgttgct gttcccgaag ctacattcac aatttttaag
    13081 tcaatatttg ctccagtttt gcggttatag atttttgcag cattttcaac atagtttgtc
    13141 agtttgccgt ttgcgacata tacttctaat gtagtggctt tttttatcat tggatctgcg
    13201 atgtttgctg ttacttcttc gttagttttt ccacatgcac ttaagctgaa taatagggta
    13261 atgtttagca aagctatcac caatttgtat ctttttttca tgtctatccc ttctttcttt
    13321 tcaagttctt atataaaacg ctttcactta tgtatattaa cggaattagt aacacaaata
    13381 tttatataaa aaaatatttt acttgtcgtt ttggcttttc atttttgtta taatagtcat
    13441 taattataaa tagaaagctt tttatataaa aagagaaagg aaagtggttc ttttgttaac
    13501 aaacaaccaa tcctccaatc ttgtcaaaaa aacactaacc tattccatag atacccatct
    13561 tcatttatct gctgctccct attttcgact actaataaat gaaagcgcta atttatatgc
    13621 tgaatttgca cataaatcta tagaactaga taatccttcc cttttattca ttgataaaca
    13681 gcaagaagtc caaatctttt ctactcaaaa ggaaacacaa acttttatca ttatccagtt
    13741 ttattttgat gagcaattat tagcacatat tacgggacaa tctttttcta tagatcaaga
    13801 aataacatct atcattactt ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca
    13861 tagtaagcca gctatttcct atgatcagga aatcaattct tttttcatac tatgccaaca
    13921 gatgagcatt catttgcttt ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc
    13981 ttccttacca aggcattatc aacaggaact tgctttattg acaatacctt caaatgagaa
    14041 agtcattcct aaaaaaaagc atattcttta ttcagatgaa ttagttgatc caatcatacg
    14101 atatttagaa gaacatcttt cagaaaattt tttgcttgaa caattagccc aaattttttt
    14161 tatcagtcca gccagcttaa aaaaacattt tagaaaagtg aatggaaaaa gtgttattag
    14221 ctattttaaa gaactaaaaa tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac
    14281 ctatacagaa attgcagata tattaggatt tcattcgatc catcattttt cttcagcttt
    14341 taaaaaatat acagggttat ccccttcaaa gtattatcta tccttaaaat agaaaaaaat
    14401 ttttaggttt tatccacaaa ttatttctct atattttctg tctattcaaa ataaacaata
    14461 attttccggc acaattattt gctgtttttt accataacat agtacgatca acgtagaaaa
    14521 ggagatgttt ttatgaaagt tgttgtcatt ggcggtgtag ctggcggtcc ttcatttgcc
    14581 actcgtttcc gtcgattgaa tgaagcgcac gaaatcatta tctatgaacg cggagaaaat
    14641 atttcttatg caagttgtgc tttgccttat tatttaggag gtgtgatcac tgaccgtgac
    14701 tcgctgatcg aacgtacacc agaaatattg aaaacaaaaa acaacatcga tgtctttact
    14761 aaacacgaag taacagcaat cgatccttct actaagcgat taacagttaa agacctatcc
    14821 acaaatgaag aaataaaaac agattacgat aagttgatca tctcttctgg tgctagacca
    14881 gattatccgg atattcccgg agtttttgaa gcagaaaacg gctttgtcct ccgtagtgtg
    14941 acggatgcgg atcgaatcaa atcgttcctt gaagaaaaaa atccacaaca tgtcgtcatt
    15001 cttggtgcag gtgttatggg tctggaatta gctgagaatt tcaagcatcg cggcttagac
    15061 gtgactttaa tcgatcaatt gccacaagtc gctttccctt atgatccaga aattgctaat
    15121 ttagtttatg acaaattgct gaaagaagga ttagccgttc atttagaaac aagagttact
    15181 gagatccgtg ataaaggtcg agaaatagta ttatcagatg gctccgtcct ttctgctgat
    15241 atgctaattt ttgctgttgg tgtttctccg aagaatgaag tggtgaaagc aaccggcata
    15301 caattatcag atacaggtca gatcattgtt gatgaccagt tacaaaccaa tctgccggac
    15361 atctatgcga ttggcgatat tatcgaaacg actagtgtag tgactggtca gccgatccaa
    15421 agtatgcttt ccagtgcggc caatcgtcaa ggacatatgc tggcagatat tttaaatggt
    15481 acgcctatgc gctatcgcgg atatattggt gcaggtgtcg caaaaatctt tgatcataca
    15541 gcaagttatg cgggaatgac agaacatgaa ttaaaagcat caggcataac aaattataaa
    15601 actgttttta tcactccctt tgaccatgcc tatttctatc cagaagctac aagattaaat
    15661 ctaaagctga tttttgatgc agatagcggt cgtattttag gtggacaagc atttggagaa
    15721 aaaggtgtcg ataaacggat gggagaactt tctgtagcaa tcaccggaaa cttgacagtc
    15781 tttgatttgc ctgatttgga gttgccttac tctccaccgt attctactac ccgtgacccg
    15841 ttgaatatag ctggttatgt cgcaatcaat caaatgacga atatcgtaga aacgatcaaa
    15901 gcaagtgata tacccgaaaa cgatttgaaa gaagcgttct ttttagatat acgtgaacct
    15961 aataaagcac cttctggcag tatctcggca acaaaaaata ttccaatgaa tgagttacga
    16021 gatcgaatca atgaaatccc aaaagataaa aaagtttaca ttactttcag aagaggattg
    16081 aatacttata cttctgcccg aatcttggca ggtttgggta tcaaagcgat tttgattgaa
    16141 gaataaaaaa acgaccgcgg tgggtagccg cggtctagga gttattttta cttgaggagt
    16201 aaaaatgaaa aagtatatag ggttgttggt tggtatgtat atagaataga agataaatgt
    16261 gaagaaattg tgcattattt cttcacattt attaggttat agtttaagaa agtttaaaca
    16321 ttgcgcaatt taattatttt agctgtttct tttcataaaa aaacatgttg tgactctgag
    16381 acaaatcata gtccatagtg atgtctctag agtaactacg cctaatatta cgaggcatca
    16441 tattaatcta tcagcactag tacctaaact agcatccctt tcgaatattt tttcttatat
    16501 aataaagttt gctttcactc ctctaatctc accaaatcga ctggcttgcg ttgtgtgata
    16561 ctaggaactg attggtttag aacgactttt ctattgtaat catatttttc gccatcctcg
    16621 ttgtcttcta atccttcaat gaatgatccc taatgtgtgt attaaataat ttaatttttt
    16681 tgttcctatt tatttaa
//
LOCUS       DACJGB010000068        16543 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.069, whole genome shotgun sequence.
ACCESSION   DACJGB010000068 DACJGB010000000
VERSION     DACJGB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.069"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..77)
                     /locus_tag="IYI34_002277"
     CDS             complement(<1..77)
                     /locus_tag="IYI34_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAR0560122.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYS"
     gene            complement(79..276)
                     /locus_tag="IYI34_002278"
     CDS             complement(79..276)
                     /locus_tag="IYI34_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAR0560123.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(670..2397)
                     /locus_tag="IYI34_002279"
     CDS             complement(670..2397)
                     /locus_tag="IYI34_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR0560124.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2390..3583)
                     /locus_tag="IYI34_002280"
     CDS             complement(2390..3583)
                     /locus_tag="IYI34_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAR0560125.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3770..4414)
                     /locus_tag="IYI34_002281"
     CDS             complement(3770..4414)
                     /locus_tag="IYI34_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0560126.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4508..4642)
                     /locus_tag="IYI34_002282"
     CDS             complement(4508..4642)
                     /locus_tag="IYI34_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAR0560127.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4644..6776)
                     /gene="feoB"
                     /locus_tag="IYI34_002283"
     CDS             complement(4644..6776)
                     /gene="feoB"
                     /locus_tag="IYI34_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAR0560128.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6773..7246)
                     /locus_tag="IYI34_002284"
     CDS             complement(6773..7246)
                     /locus_tag="IYI34_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAR0560129.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7647..7871
                     /gene="nrdH"
                     /locus_tag="IYI34_002285"
     CDS             7647..7871
                     /gene="nrdH"
                     /locus_tag="IYI34_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAR0560130.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8030..10189
                     /gene="nrdE"
                     /locus_tag="IYI34_002286"
     CDS             8030..10189
                     /gene="nrdE"
                     /locus_tag="IYI34_002286"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAR0560131.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10239..11204
                     /gene="nrdF"
                     /locus_tag="IYI34_002287"
     CDS             10239..11204
                     /gene="nrdF"
                     /locus_tag="IYI34_002287"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAR0560132.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11293..12432)
                     /locus_tag="IYI34_002288"
     CDS             complement(11293..12432)
                     /locus_tag="IYI34_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAR0560133.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12741..13454)
                     /locus_tag="IYI34_002289"
     CDS             complement(12741..13454)
                     /locus_tag="IYI34_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAR0560134.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13708..14409)
                     /gene="nagB"
                     /locus_tag="IYI34_002290"
     CDS             complement(13708..14409)
                     /gene="nagB"
                     /locus_tag="IYI34_002290"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAR0560135.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14643..16175
                     /locus_tag="IYI34_002291"
     CDS             14643..16175
                     /locus_tag="IYI34_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR0560136.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16366..>16543)
                     /locus_tag="IYI34_002292"
     CDS             complement(16366..>16543)
                     /locus_tag="IYI34_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0560137.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5131 a         3415 c         2867 g         5130 t
ORIGIN      
        1 tcagaatatt taggatcttt acttatagat atatcattat ataattcatg aacaatttgc
       61 ttagcatttt ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat
      121 agacattcct gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt
      181 gcaatacact ccatttccat aatattttcc actatgagtg gtacccccat agataagttg
      241 tgtctcttta atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg
      301 tttttagact attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt
      361 agcaaaataa ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc
      421 tttaatattt tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca
      481 tacctacgat cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt
      541 tgaacgtttc gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg
      601 aacatggagc ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg
      661 tctgtctttt tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt
      721 tgctgcttgc tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt
      781 ttctgaccaa tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc
      841 ttttcgaacc atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa
      901 gtatttcaga tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg
      961 ttcgtgtttt ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc
     1021 tactttccag tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac
     1081 accagctcct ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat
     1141 cgaatttaca tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc
     1201 acctaggtct tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag
     1261 ccaagctgaa accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt
     1321 atctaatggc gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt
     1381 tgttgaagca acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt
     1441 ggcagatgaa aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat
     1501 gatcagtcct tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt
     1561 tggctggttc aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac
     1621 cccgttgaca aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc
     1681 taagccgggt gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt
     1741 ttgattgatc cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt
     1801 ttgacgactt gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg
     1861 aatccctaaa ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac
     1921 ccctggaacc atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg
     1981 cattgtctgc ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc
     2041 catcaagatc cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag
     2101 tgtgacagta ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat
     2161 taatgtgaac aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa
     2221 taacaagata aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac
     2281 tgcctgtcca atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc
     2341 tgtgatattt tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt
     2401 cactcacctc gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt
     2461 ttaattttcc atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa
     2521 tatcacttac caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca
     2581 atggacgttt ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt
     2641 gtctctttct gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg
     2701 caacgtttgg tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag
     2761 ccgtaagcac tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg
     2821 gattggtcaa tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg
     2881 cgattctgtc cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt
     2941 tttgaacatt ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat
     3001 caagagccga gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg
     3061 ctaggccaac ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg
     3121 aaagcaaact agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta
     3181 caccacggac ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac
     3241 cgaagttttg gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc
     3301 ttaacttcga tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac
     3361 gattcaatag acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga
     3421 tttctccttc ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct
     3481 ttaaaatctc cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt
     3541 taccaaatat ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt
     3601 atgtaaattc atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca
     3661 ctttgtcaaa aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt
     3721 gtaaataaaa ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa
     3781 gctgatgctc cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa
     3841 aatgaacgat gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt
     3901 cattatgaga aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa
     3961 ttgaacggtc cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat
     4021 tcatcttatg tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat
     4081 tcagctcatc ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg
     4141 attgtacatc ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg
     4201 ccccacttcc atgccgtact tccatgattc caagatctac cagcacattg atagccttgc
     4261 gggtagtctc aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct
     4321 cacccacttt atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt
     4381 agcgcgggcg gacgattttt ttctttccag acattttctt tcctctttct ttttttactt
     4441 tccctcatct tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa
     4501 cgactgatca tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac
     4561 aattcttacc ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca
     4621 cacttaataa gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac
     4681 tggttcttgg attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata
     4741 gcaagatacg tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc
     4801 acaaaactaa ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc
     4861 ttggcattca tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa
     4921 ttgaaggcta acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag
     4981 atttctctgc cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata
     5041 atattttctt tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa
     5101 ccaagcggct gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct
     5161 tcagaaacag gttgagcata aaaattataa gaagagctga accagatcac gatactagaa
     5221 acaaagataa tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt
     5281 aggaccgctg accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta
     5341 tctccagcaa acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata
     5401 ccaagaaaat aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact
     5461 aaggcaataa tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa
     5521 cgacgatctt gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct
     5581 gtagcaatca gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga
     5641 tccatgacaa aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa
     5701 aacaatataa tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg
     5761 ttcaagatca gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca
     5821 tgttctggta accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct
     5881 actgtttgaa tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct
     5941 aaccaacgat tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca
     6001 tttgacacaa cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga
     6061 atcgtctcac tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct
     6121 ttttgctgag aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat
     6181 ttcattgcat accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt
     6241 aaagctgctt ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt
     6301 gcagactcct gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt
     6361 ggtaaaactg gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct
     6421 tttttgatga catccatcat attcagacca atgatcatag gaatgtctaa atccaataac
     6481 tgcgtagtta aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt
     6541 tcaggctgtt cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac
     6601 aaagaataga tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct
     6661 tctttttttt ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag
     6721 agattgaaca agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt
     6781 tttcctccct ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc
     6841 ctcgcactgt cagttcgatt ggatctccca gaggggctag ttttcggata actattttta
     6901 catttcgcgt caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg
     6961 ctacgacgat cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg
     7021 aagcagtctt tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat
     7081 gactttttac cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc
     7141 cggcaacgat cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt
     7201 cgacttgata aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct
     7261 tgtgaaataa atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac
     7321 gcaaataaat gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat
     7381 acttgtgata ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt
     7441 cttcatagtt ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg
     7501 ttttcagctc ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata
     7561 tgtagtgttt gttgtttgac ttttttacta agagttttat actatctatt atagataata
     7621 agaatgtgtt agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta
     7681 tgcaatgtaa gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca
     7741 atatcgatga acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc
     7801 ctgtcattac ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac
     7861 ttgccagcta acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga
     7921 cagcaccagt ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat
     7981 gatttgcaat caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa
     8041 aaatttacaa gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg
     8101 tcaaatccct ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa
     8161 accaaatacg atgacttttc catctattat ggaaaaaatc aattacttga tcgatcaaga
     8221 atatatagaa gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca
     8281 atttttggac gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc
     8341 tcaatatgcg ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt
     8401 agccttcaat gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga
     8461 cgaaatgatc catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa
     8521 acgtcggggc gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat
     8581 cgggcgctca atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac
     8641 tttatcaaat cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg
     8701 tgtcgttcca gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca
     8761 gcgtcaagga gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct
     8821 ttcagcaaaa aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt
     8881 gatcgtacct gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag
     8941 tccatatagt gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga
     9001 gtatgatcat ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct
     9061 tgaaaatgaa atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga
     9121 tacagcgaac cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga
     9181 gattttacaa gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg
     9241 aacagatatc tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa
     9301 ctttggtcaa tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga
     9361 tatcgatgtc gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg
     9421 tgccatgggc ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc
     9481 attagacttt acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa
     9541 caatatcgct cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga
     9601 cggcacttat ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa
     9661 agcattgttt gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc
     9721 agtaatgaaa gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat
     9781 ttcatatatc aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg
     9841 ccaagagaag aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat
     9901 tccgtattac acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaac
     9961 ggcacaacaa cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc
    10021 agaaggcatt tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt
    10081 aagacattac gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga
    10141 tgatgcagaa gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata
    10201 gattcgctta ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag
    10261 caataaactg gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag
    10321 agcaattttg gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat
    10381 tgtctcaatt ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata
    10441 ctgtccaatc tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag
    10501 aagccgtttt gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta
    10561 tttttagtac actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga
    10621 atccttatct gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc
    10681 tagaaaagaa aattgccagc gtctttttag aaacattctt gttctattca ggtttttata
    10741 ctcctcttta ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa
    10801 tcatccgaga tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca
    10861 acgagttacc tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg
    10921 agctttacga aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag
    10981 aagaagtcaa gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg
    11041 atccactctt tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa
    11101 caggcacaag caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg
    11161 tagaagcgat gaaagacgac gattatttga ttggattgga ataaggattt attttaattc
    11221 aaaaattttt cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac
    11281 tcttttttat gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga
    11341 aacatgttcc attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat
    11401 cactgcatag acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc
    11461 aatcaatacc aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg
    11521 cttgatcaaa tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc
    11581 ttgaaatgga gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc
    11641 gacatacggg ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg
    11701 atcgaatatt ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt
    11761 actgctgata taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt
    11821 acgaatcgct ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa
    11881 cataaatagc aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga
    11941 aatgattttt gagacacttg cagataaact gactaatatg atattgaaca aattactaag
    12001 agataaattt gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg
    12061 cgccaattca tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa
    12121 ttgaattgtc tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat
    12181 gattacgacc aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag
    12241 aaaaacaacg attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa
    12301 aattgccgaa agcattttca atattggact aaaaatattc ggcatttgta aaataaagta
    12361 cacgccgatg atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa
    12421 acgtgattgc atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa
    12481 gagttacgcg ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa
    12541 tgagtagacg agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca
    12601 gtcccttttt ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa
    12661 acgaaaaata taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct
    12721 attttacaac ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt
    12781 ttgtgtacgg acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc
    12841 gtatagatat ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct
    12901 tcgattacga tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta
    12961 tggataaatt ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact
    13021 gtgagtgcaa tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg
    13081 atggcgatcc ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc
    13141 gattgtttcg tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga
    13201 gcaatcagcc ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca
    13261 tctcttaatg caccactagc gatctcaact tggatcaatg caatgataca tgctcccata
    13321 caaactaaaa tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg
    13381 attgctccgc caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat
    13441 accatatcat tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca
    13501 aaaaaagtgc aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt
    13561 cctttttatg aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca
    13621 aaatatgggg agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc
    13681 acaacctctt tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat
    13741 cacaacaaca ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc
    13801 ttcaatcatc cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc
    13861 tttacttttc ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc
    13921 tttatcgaag aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt
    13981 cgttccgtca aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc
    14041 taagatttgg atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc
    14101 ttccagatct tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa
    14161 taattggaca tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta
    14221 ctcatctaag tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt
    14281 atataaagta actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat
    14341 accttctttg atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat
    14401 gatttccata gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt
    14461 tctttttaca ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat
    14521 aggaatccgt gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac
    14581 caagcaagtg attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta
    14641 ttatgagtat actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg
    14701 aaggtgtcga tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag
    14761 ccggtaaaag tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga
    14821 tccattggca aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa
    14881 cattaacaat cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa
    14941 aaattgcttt tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag
    15001 ctgggaaact ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg
    15061 ctgagatgag cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc
    15121 taagtggtgg acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg
    15181 ttctgatctt agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc
    15241 agtttcttca ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg
    15301 atcagatcac cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc
    15361 atctaaaaca agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc
    15421 atgctcaaca aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt
    15481 cccgatcaac gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc
    15541 ctccagcttc agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat
    15601 tagcagtgga aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca
    15661 atttgaccat ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat
    15721 ctactttatt aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc
    15781 cagataacac aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa
    15841 caccactaca atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc
    15901 aactagctaa agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg
    15961 gtggggaaca aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga
    16021 ttctcgatga accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga
    16081 tacagaaatt tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc
    16141 ttgtgcaacg agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa
    16201 aggagctgtg acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag
    16261 ctgaccttcg gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta
    16321 ttcttattac ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc
    16381 gagatttctc tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac
    16441 aagcccgcta accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa
    16501 tgaaccagcc gcacctccaa taaagtacat aaacataaat aca
//
LOCUS       DACJGB010000069        16357 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.070, whole genome shotgun sequence.
ACCESSION   DACJGB010000069 DACJGB010000000
VERSION     DACJGB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.070"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(141..797)
                     /locus_tag="IYI34_002293"
     CDS             complement(141..797)
                     /locus_tag="IYI34_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAR0560138.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            990..1268
                     /locus_tag="IYI34_002294"
     CDS             990..1268
                     /locus_tag="IYI34_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560139.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1287..2495
                     /locus_tag="IYI34_002295"
     CDS             1287..2495
                     /locus_tag="IYI34_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0560140.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2643..3008)
                     /locus_tag="IYI34_002296"
     CDS             complement(2643..3008)
                     /locus_tag="IYI34_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAR0560141.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3155..3928)
                     /locus_tag="IYI34_002297"
     CDS             complement(3155..3928)
                     /locus_tag="IYI34_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR0560142.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4102..4962)
                     /locus_tag="IYI34_002298"
     CDS             complement(4102..4962)
                     /locus_tag="IYI34_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAR0560143.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4962..6380)
                     /locus_tag="IYI34_002299"
     CDS             complement(4962..6380)
                     /locus_tag="IYI34_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560144.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6437..6886)
                     /locus_tag="IYI34_002300"
     CDS             complement(6437..6886)
                     /locus_tag="IYI34_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR0560145.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7331..7594)
                     /locus_tag="IYI34_002301"
     CDS             complement(7331..7594)
                     /locus_tag="IYI34_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAR0560146.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7693..8988)
                     /locus_tag="IYI34_002302"
     CDS             complement(7693..8988)
                     /locus_tag="IYI34_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAR0560147.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8985..10256)
                     /locus_tag="IYI34_002303"
     CDS             complement(8985..10256)
                     /locus_tag="IYI34_002303"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAR0560148.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10422..11291)
                     /locus_tag="IYI34_002304"
     CDS             complement(10422..11291)
                     /locus_tag="IYI34_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAR0560149.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11583..13193)
                     /locus_tag="IYI34_002305"
     CDS             complement(11583..13193)
                     /locus_tag="IYI34_002305"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAR0560150.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14386..15726)
                     /gene="glnA"
                     /locus_tag="IYI34_002306"
     CDS             complement(14386..15726)
                     /gene="glnA"
                     /locus_tag="IYI34_002306"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAR0560151.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15754..16146)
                     /locus_tag="IYI34_002307"
     CDS             complement(15754..16146)
                     /locus_tag="IYI34_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR0560152.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4668 a         3469 c         2780 g         5440 t
ORIGIN      
        1 ctcacaacct cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
       61 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggtc aaaacgctga
      121 tttcacaacc tctttgttca tcaaaagata atattgtctt ctaacaagtc catattcaaa
      181 ataacgatat gatggtcttt aacggtgata ataccatctt ttttcagttg ttttaaaatt
      241 ctgtttacac tgctagcagt tgagatgcca caaaatttgg cgatttcttc attcgtgatc
      301 gaaaaatcga tcagcatccc ttcttctgtc cgcacaccga ataaatcata taattcgtaa
      361 agttgcgtac aaacagctcc cagctttccg tttaatagca tttgctgcat ttttttcatc
      421 gaataaagca gacgatagcg gtaatactct tttacatagt gttgtagctt tgggtcttgg
      481 ttgatatttt gccaaaagcg cacacgattg attcgataaa actctgcata ttctgattca
      541 actcgaatat tatacggcga atcgatgaat tgtgaatatt cgtcttttaa aagagagacg
      601 atttcaagat ctttgatata gcgaaggtta aattctctcc catcatttga aataacactt
      661 gcttttaccg ttccctcttt caagatatac gtatgggaat cttccatccc ttcgaatgtc
      721 aaatatttat gatatccttt gcgtataatc ggaaaatcat ttttatccaa atattccttt
      781 aagactctac tatccaatga tgctccccca tccaaacttt catctaaaaa gatcatacag
      841 tactttaaaa ctttttaaag tccaaaggta ttttactaat gatattaaca tttgttaagt
      901 acctttttcc atcacttatg cgattttttt cctttttttt attcgttata ctgcagaagt
      961 aaaatttttt aagaatagag ggaaaaaaca tgaattactg gatgaacaca atcattaatc
     1021 gattagaaac agcatatcaa acaagatttg atatgaaagc ttctttagtc tttttaaatg
     1081 atgcgtatca aaactcgatc gaattgatca aagcagtgga tgagaatcca acaaacgaat
     1141 gcgaagaatt tttaaatctg tttatgtcta caagagattt atttattcgc caacttgttg
     1201 atcgctatcc atccaattat cacgatgtag aagttcaaat acaaaaatta aaagcatatt
     1261 cagcttaagg ggaatgtata acatacatgg aaaagaaatt atttaataaa tattttattg
     1321 gaatcacatt gatcaatttt attgtctacc tgatttatta tctgttgatg gtcatcatgg
     1381 cagtcattgc gcaagacacc ttacacgcca gcttgagcca agcaggtttt gcttctggga
     1441 tctatatcat cggaacattg ctggcacgct tattcatggg gaaacagtta gaactatttg
     1501 gtcggaagtt taccttaaga ggcggcgcga tcttctatct cctttcaaca ttagcatatc
     1561 tttatacgcc aactatcggg gcattgtatt tagttcgatt cttgaatgga ttcggctacg
     1621 gtacagtttc tacagctaca aatgctatcg ttactgcata tatccctgca tctaaaaaag
     1681 gggaaggcat caattattat ggattaagta ctagtttagc tgccgctgtt gggccatttt
     1741 taggaatgat tttgctgaat atgactgatt ttcgtttcat tatctggttt tcaattgtac
     1801 tggtcttctt tgttacgatt gcctgccttg tctttcctgt aaaaaatatt ccattatctg
     1861 aagaacaaaa aatatctctt cgctcatgga atatcaatag ttttattgaa aagaaagcac
     1921 ttttcattac aggaattgcc tttttgatgg gtctttctta ttcaagtgtt ttatcatttt
     1981 tatcttctta tacaaaagta atccacttag ttgctgctgg gtcatttttc ttcgtcgtat
     2041 atgctttggt catcacattt acacgtccat tgactggaag gattttcgat gtcaaaggag
     2101 aacaatacgt gatgtatcct agctatttat tcttaacagc aggattattt ttactgagtg
     2161 taacgacgaa tagtgtgaca ttgcttatct ctggagcatt agtcggccta ggttatggta
     2221 cgtttatgtc aaatggacag gctgtttgtt taaaaatcgt ccgcgaacat cgaatcagta
     2281 ttgctttatc tacctacttt attggtttag acctaggctt aggcgttggt ccttatttga
     2341 tggggatgct aaaatctctt acttcattta gaggattgta tgtgattgcc ggagtcattc
     2401 cattgatatg tacagcactc tatcttgtta atggccgaaa acatagtcat gaagcagagg
     2461 gcgaattgat tgaatcattg aaaaatgaaa tttgatcttg atgaatagat aaaaaaaacc
     2521 gttcagatct gaaaataatc tgagcggttt tttgagtaaa aatagtttat gattattctt
     2581 aattaaacat ctatttattt ttaaagcttt catttatcga taagaatagt ttaactgttg
     2641 atttattcca ttttccgttc atctggtacg atgccttgac ttagcaacca cttgtggaag
     2701 aaccattctg ccacaccgat caatagagca ggaatcaata ctttcatcca aatatcgact
     2761 gtatcattca taaaatatag atagagccac aagataactg cagctgaacc agcatctgcg
     2821 atcgtcgctg cgatgttccc tactttacgt aagatcaaca gatcccctag cacatagatc
     2881 atgactacaa taattacccc aacaatcgtt gaatccataa agctaacacc gtataaacct
     2941 gtcagcacga cccacagaat cgcccagatc atgattgcct tcactaccaa cgccttcaca
     3001 tgtttcattt tctgcactcc tttctcctgg ataaaaaagt aagattatct aacaagataa
     3061 ccttttcatt attataccat cggctgagag aagcgagcaa agataaggtc ctatctaaaa
     3121 atgaggaagc cacttagaac attcacgtaa taatttatcc gtacagacat tcgatatggt
     3181 gttcctttac tttcgcatac tgttcttttg tcagttcttt acttgtcacc agataatcga
     3241 tttcatgaaa agcaatggct tgatgcatga atttatgtgc aattttttgc tgtgtaacta
     3301 aaacggcaag ccggtctgat tgttttctaa aagctttttt gaccattgcc tctccttcat
     3361 gagtttctgt tactccttgg ctgaaatcta gcccgcgaca actcattatc gctaaatccg
     3421 cataatatga caatgcatcg ttataggctt ttgaaccgtt gattgttgct cgtttcggaa
     3481 caattgcccc tcccaataca gatacttttg ccgttgtata ttcactcaac atagcagctg
     3541 tgacgatgcc attcgtaatg atctgcaaac catcaaatct tgctaggttt ctgactaagt
     3601 gtaaagaggt tgtactggaa tccaagaata agaccatatc atctcgaatc aaagaagaag
     3661 ctgtatctgc aatcttacgt ttttctgttt cttcttctaa tttggtgatc ttcatcaggt
     3721 aaggcacgtc aatttttcga ttgtcaataa gcatggcgcc accgcgaact ctacggatca
     3781 atccactttt ttccagaaaa acaaggtcac gtttaatcgt ggcttcgcta taatttacta
     3841 actcgatcaa atcggtgact cttgcaaatt ggttgttttt tagataattc acaatctcct
     3901 gctgtctatt ctccacttca ttcaacataa aatcacccca tttactttat atagtaacag
     3961 gaaaaaagag gttgtgacag aagtgattgg cttcgagaaa taagaagaaa tttttcgaaa
     4021 aaactacttc tgaacatcgt gtgtggttaa gagggtatga caagactatt gtcataccct
     4081 cttgaccaca catttttatt tttagtataa actagccttg cctactgtgt tcaaaatttc
     4141 cattttatgt ttgaccactt cttgtacctt tttattagct gctggataaa ttgcattcgg
     4201 ctcgtacatc gatggatttt cactcaatat ttctctcaaa gcattgaaaa agacgctttt
     4261 aagatctgag ctcaaattca ctttggcaac tccgtatttt actgcttcgc tgacttctgc
     4321 gtccggatta cctgaaccgc catgtaaaac aaatggaaga tcgatttgtt ggttcaattc
     4381 tttcagtaag tcgatattta attttggagt tctgtttttc ggatacaagc catgcgctgt
     4441 tccaattgct acagcaagtg tatcgattcc tgtttctttc acaaaaatct tcgcttgatc
     4501 aggatctgta taaatgattg tttcagcacc tccttcagca gaaccattat tcccaatcgt
     4561 tccaagctca gcctccactg agacatttac cgcatgagct aacgcaacca cttttttcgt
     4621 aatctgtaca ttgtcgtcga atgatttcaa agacgcatcg atcatcacgg aagaatagcc
     4681 gtttcgaatc gcccgtaaga catcttctat cgtaccgcca tgatccaagt ggataacgat
     4741 cggcagttta cttgtataag cataggctct tactgccgca acaaactcat cacttaaata
     4801 ggcgatttca tcaggatgaa tttctaagat tactggtgct tctaatctct ctgcttcttc
     4861 gatgattgct ttcatcatat catagctgca aatattgaag gctggtactg caaattgatt
     4921 ctctttagcc actttcaata actctttcat tgtatataac attacgcttc cacctcattt
     4981 gttagatttt catttttcgt ccacttgtct tgttttttct ctttcactgt ttttttcaat
     5041 agtccaagca taaaggctgt agtgactgta cctgatgcaa ttgccaagac atacataggt
     5101 aaattgttca taacatttgg aattagcatc acccaaatcc ctccatgagg tgccattgaa
     5161 gtaactccta ctgccatgga aatccctgca gcgactgctg aacctgtcat caaactaggt
     5221 atcacgcgga tcgggtcaga taccgcaaac ggtatagcac cttctgtaat atacgaagcg
     5281 cctaaaatcc agcatgattg acctgaagtc cgttcttctt tggtaaattt atgtttgaat
     5341 aataaagtgg ctaaagcaat acctagggga ggtgtcattc ctgcagccat acaagcagca
     5401 atcggtgcat ttactccagc tgacatcaaa ccgatcgaga aagtcgaagt cgatttattg
     5461 atcggcccac ccatatcagc tgccatcatg atcccgataa gtaatccgaa caatagcccg
     5521 gatgtatttc ccaagctatt cagccaattt gtcaaagcat ccaacaccca tgtgatcggt
     5581 tgttcaatca caaataccat caaaagccca ataattaaga tcgataaaaa ggggacgaca
     5641 atcagctgat aaatagcaga aaattctttt tttacgctaa ttttatttgc cagaaaatca
     5701 cagatatacc ctgctagcac gccaccaatc attccgccaa gaaaacctga accacctgta
     5761 gaggccagta atccggcaac aattcctggg gcaaaacctg cttttccagc aatcgatgta
     5821 gcaatccccg cgcctaacgc aggaaccatc atggcaaacg ctgtatctcc tccgatcctt
     5881 gaaaatgcag ctgctaacac attatagtct gctgagttgg gatcagccgc tgtaatccca
     5941 aaggcaaaac tcagtgcgat gatgattccg cctgcgatca caaatggaag catgtaattc
     6001 acaccattca tgaaatgatt atagattgtc ggctttttct catttgcagc tggttctttt
     6061 tcattagaca caacgaatac gccaatatct ctcaatgctt cgtctattac tacttgcggt
     6121 tctttgattg ctcggcttgt agagacacgc tttacttttt tcccggcaaa tcggtccaat
     6181 tcgatttctt tatctactgc aagaatgatt gcatctgcct ctttgatttc ttgttcagat
     6241 aactgatttt ctgtttttac accttgtgtt tcgaattttg cttgatggtt tagatttctt
     6301 gctgttactt ctaatttttc tgccgacata taagtatgag caattccagt cgggcaagca
     6361 gttacaccaa cgattttcat gattattttc ctcctttttc ttcactatcg atactcggac
     6421 aaagactcta ctattttcat tcttgactaa tctgtctgat gatatctgct ggcgtagtgg
     6481 cttcttttat tccctttcta aaatctgcat tcatcaatcg tctggagagt gtgctgagaa
     6541 tctctagatg catatctgct gatttttcag gaactacgat ggcaaaaata tattcaattg
     6601 gcatttcatc tatcccgcta tagtccgtta atttggctgt atgaaaaaaa cacagtgctg
     6661 gttttgtcac atatttgctt tttgcatggg gaatcccaaa tccatcttct attcctgtgg
     6721 agatctcctc ctctcgtttg aatagtgcct taacgacttt ttgaacattt cttaccttat
     6781 ttgttttttt caacacatcc cctattcgct caaataatga tccttttgaa tctatcgatg
     6841 gatcgaaaac aatcagttct tctgcgatca attgatcaaa cctcataaaa aacctctccc
     6901 ccacaaaaaa tgttaacgtt ttcttggaac tgaggtcagt atagcatcgc tagctgtacc
     6961 gttcaatatt atgaatgaac agaggaaaaa gacccatcat ccgcgtgtat aagtcattaa
     7021 aatgaacgat ctagctcaca aacaatcgca agtctataac aaaacaaaaa gccttccttg
     7081 aaaggaaagc ttagcttcta attttaggta aatggttttt tacttcgaaa taacccctct
     7141 tcatttcgtt aatataaggt gtttttatca agtggataat taaaactata tactagtttt
     7201 tcattgtttt gtgcattatg atcctacgac tttttcagca tcaaacgaag ttttaaattg
     7261 gcaaaaaaca taaaaaaaca gactatccct atcagatagt ctgctaaacg ttgagttatc
     7321 agcgttttta ttattccgca gcgtttgcgt ctttgagacc gtattttttg ttgaaacggt
     7381 ccacacgtcc gtctgcttgt gtgaattttt gacgtccagt gtagaatgga tgtgagtcag
     7441 aagttacttc gacacggatg actgggtatg tattaccgtc ttcccattca actgtttctt
     7501 gtgaagtttt tgttgaacct gacaagaatt tgaaaccagt tgttgaatcc atgaatacta
     7561 ctgggtggta atctggatgg atgttttctt tcatgatttt ttcgctcctt tgccctgctc
     7621 catttgctga accagagttg atttgtcgat ttacaacatt aacatcttaa catgtttcag
     7681 tcaaggttgc aattatcttt tcagatttct ggtcggattt tttttaccaa aggagacatc
     7741 ttgaaattct ttgaaaaatt gctgattgtt ttttgtttta cgcaaaaagc ggatcagttg
     7801 ttccgtatat tctaatgaat ctccagtcat attttttcga agtttccaaa tttcttctaa
     7861 ttcttcactt ggcatcaata attcttcttt tcgggtacct gattttttga tatcgatggc
     7921 tgggaagatc cgacgttcag ataattcacg tgaaagttgt aattctagat ttccagttcc
     7981 tttgaattct tcatagatca cgtcatccat acggcttcct gtatcgacta aagcggtcgc
     8041 taaaatcgtc aaactgccac cttcttcgat gtttcttgct gccccgaaaa atttcttagg
     8101 tttatacaat gcggctgggt caatcccgcc actcaatgta cgtccgcttg gtggaacgac
     8161 taagttatag gcccgagcaa gtctagtaat actatccatc aagataacga catctcgttt
     8221 atcttcgact aaacgcattg cgcgttccaa tactaattca gaaactcgag tatggttttg
     8281 cggctgcaga tcaaatgtag aagataccac gtcgcctttc acgcttcgtt ctagatccgt
     8341 tacttcttcc ggtcgttcat caatcagtag aacgattaat tctacttccg gatagttttc
     8401 ggtgatacca tttgcgattt cttttaatac agaagttttc cctgcttttg gcggtgcgac
     8461 gatcaatcca cgctgaccaa aaccgattgg agaaaagata tcgatcattc gggtagatag
     8521 tctttgcggt gttgtttcca gttgaagctg tctttgcgga taaagaggtg ttaaggctgg
     8581 aaaatgcgga cgttcttttg cttcttccgg atcttttcca ttaacgcttt ccacgtgcat
     8641 caaaccataa taacgttccg attctttagg tggacgggct tttcctgcca ctttgtcccc
     8701 atttctcaaa ctgaatcgtc tgatttgtga ggcagaaata tagatatctt ctgcacttgg
     8761 tccataattg attggccgta aaaaaccgta accatcttga ccaacgatat ccaatattcc
     8821 ttccatataa taaaatcctt gtttctctgc ttgtgcacga atcacagcta gagataattc
     8881 ctttttattc atcttactgt aataaggaat tttaaattct ttggcataac tataaacttc
     8941 ttttaacgtt ttgttctcta gttctgccat tgtcaaataa tcgctcattc agctcccact
     9001 tcctctatca tttcaattga tgcccctaag gctgttagtt tctcgataat atgatcataa
     9061 ccgcgtaaga tataatctac attgtaaatc gtcgttgtcc cttctgccaa tagtccagca
     9121 atcactaaac aagcacctgc ccgaagatca gaagccacga cttctgtgcc gtgaagttta
     9181 tttggaccat tcacgatgat catatttcct tccacggtaa tatcagcgcc cattcgtgct
     9241 agttccggca catggttcac acgtttcgca tagatcgtat cgatgatttc tgaagtgcct
     9301 gtagtcatca atagtaacgc agtaagtggc tgctgcagat ctgtggcaaa tcctggataa
     9361 ggataggtca tgatgtttgc gggttttaat tctttcgagg ggtaaacttc gatttcatct
     9421 tcgctgattt tcatatggac accaatttcc tgcaattttg ctataaagct ttccaaatgt
     9481 tcaaagatca catttttcac tttgatacca tttcctactg cagctgctaa tgctagatag
     9541 gttcctgctt cgatgcggtc aggaatcatg aaatgccgac agccatgtaa tgtttcgact
     9601 ccttcgatac ggatcacatc tgtacccgct cctcgaactt ttgcccccat attattcagt
     9661 aaagttgcca catctatgat ttccggttct cgtgcagcgt tttcaattac tgtctgtcct
     9721 tttgctttta cagcagctaa catcacattg atcgttgctc cgactgaaac cacgtccatg
     9781 aaaatccgat tgccacgcaa tcctttattt tctgtccgta aatacatggc accatgttca
     9841 tttgtcactt cagcacctag agcttcaaat cctttgacgt gtaggtcaat cggtctaggg
     9901 cctaaataac agccacctgg caggcctact accgcttcgc caaatttacc aagcagtgtt
     9961 cccataaaat aataagaagc acgcaaacta ttgatttttc cgtgcggcat cggaatcgat
    10021 acgacttctc gaggatcgat ttctagtatg ttgttggaaa aatggacagt tgcgcccatg
    10081 atctctaaaa tttcaattaa tgaatgaaca tcttgtatgt ccggcacacc ttccaagatc
    10141 actggggaat cagctaagat tgcagctgga atcaatgcga ctgcactatt ttttgcaccg
    10201 ctgatcgtta cttcaccagc taaaggtcgg tttcctttga ttacgatttt tttcatctgt
    10261 ttccaccttg tttattaatc ttttttcatg gatatgcttg tattttatca tagataaaaa
    10321 aaaagaaaca agcatggtct gccgttttaa gtaaaaaaga atggagagcg acttcaaaag
    10381 tcgttctcca ttcctatata tcgtatctgt tatgtggatg cttacgcttt gttagctgaa
    10441 ccgaaccagt cgatatgaga tttagcatct tcaacgattg ctgctttacc tggagctaat
    10501 aatttacgag ggtcgaatcc tttaccttct ttatctttac cagcttcgat atatttacga
    10561 gtagctgcag caaatgataa ttggaattca gtgttaacat ttacttttga gatacccatt
    10621 gagatagctt tttcgatttg atcttgagga atacctgatc cgccatgtaa tactaaaggc
    10681 atgtctggac ctacagcatc agcaattgct tgtaagtggt caaaagctaa tcctttccag
    10741 ttttctggat attgaccatg gatgttaccg ataccgcaag ctaagtagtc gatacctgta
    10801 gcaaccattt gtttgcattc ttcaatatca gctaattcgc cgttaccgat gattccgtct
    10861 tcttctccgc cgattgaacc aacttcacat tcaactgaga taccttttgc atgtgctttt
    10921 tcaacaacgt ctttagctaa tttcaagttt tcttcaaatg gtagatgtga accatcgaac
    10981 attactgaag tatagcctaa ttcgatacat tccaaagcag cttcgtaatc accatggtct
    11041 aagtggatag caactggaac agtgatgttc attgcttcaa ctaaatcagt gattaaatct
    11101 ttacagattt tgtaaccacc catgtattta gctgcaccca ttgatgtttg gataagtact
    11161 ggtgcttgtt ttgcttcagc cgcttcaagg attgcttgag tccattccaa gttgtttgtg
    11221 ttgaatccgc cgacagcgta accgccttta cgagcagctt ttaaaaattc agctcctgat
    11281 actactggca taataaaatt cctcctaagt tttgtaaaac taaatttgtt aagacaattc
    11341 ttaaccaacg accctatttt accaaacatt tcggtagttt tctactaaaa tcaatgaaaa
    11401 tgaaaatttt caagaagttt tttgcaaaaa ataaaatatc caatagcgct ttaatcgaaa
    11461 aaagtctttt gctttctatc ttttttccta caaataataa caaatcagaa aaagagaaaa
    11521 gtgacaagaa aattgcttat ccccttctct tgctactttt ctctttctat caagttcaaa
    11581 aattattttg tttcgtgatc tgctaatgat gctccgacaa atgctttgat taatttttgc
    11641 gggcgatttg gacgagaaat caattctggg tggaattggc atcccacaaa gaattttttc
    11701 tctggcagct ctacgatttc tactaaacgg ttatctggtg agacacctga aaagactagc
    11761 ccttgttctt caaacaattg acgatatttg ttattgaact catagcggtg acggtgacgt
    11821 tcttgcacaa catcggctcc atcatatgct tcagccgtct tcgttccttt tttgatttta
    11881 catgggtata atccaaggcg taaagttcca cccaaattct cgatgttttc ctgatcagcc
    11941 attaaatcaa taatattgtt tgctgtatct ggattcgttt cagctgatga cgcatcttct
    12001 aatccaacga cgttacgtgc aaattcgaca caagccatct gcataccaag acaaatacct
    12061 aaaaacggaa cgtcattttc gcgagcataa cgaatcgctt cgattttccc ttctactccg
    12121 cgatcaccaa atccaccagg aaccaagatt ccatctgcgt ctttcaaacg ttcttctaca
    12181 ttttctctcg ttaattcttg tgctttgatc caatcgatct cgatgtctga attataggcg
    12241 aaaccagaat gtttcaatgc ttcaactact gagatatatg catctggtaa ctcgacatat
    12301 ttaccgacaa gcgcaatccg tacttttttc ttcaagttca agacacgttc ttctaattct
    12361 ttccattctg tcatgtctgc ttctggtgta tctagtttta aatggtcgca aacaatttga
    12421 tccattcctt gtgcttgtaa aactaatggg atggagtaaa gtgtatctac atctggcgac
    12481 tcgataactg cttctggttc gacatcacaa aattgtgcca atttgttttt cacgctttga
    12541 gaaacaggtt gctctgtacg taccaccaaa atattcggtt ggatacctaa accacgtagc
    12601 tcttttacac tgtgctgcgt tggtttagtt ttcatttctc cagcagcttt caagtaagga
    12661 atcaatgttg tatggatata cataacatta tctgtaccga catctgcttt catttgacga
    12721 agtgcttcaa ggaatggcaa agattctata tcccctactg ttccccctac ttctgtaatg
    12781 atcacatctg aatcagtcat tttagctgct cgcatgattt tttctttgat ctcatttgta
    12841 atatgaggaa taacttgaac agttgctccc aagtattcgc ccttacgttc tttacgcaaa
    12901 acttcagaat agatttttcc tgtcgtcacg ttggagtatt tgttcaaatt gatgtcaata
    12961 aaacgttcgt agtggcccag atccaaatct gtttcagcac catcatctgt tacgaatact
    13021 tctccgtgct gatatgggct catcgtcccc ggatccacgt tgatgtatgg atcaaatttt
    13081 tggatagtta cttttaagcc gcggtttttc aataagcgtc ctaaagaagc tgcaacaatc
    13141 ccttttccga ttgatgaaac gacaccgccg gtcacaaaaa tatattttgt cataagttca
    13201 aaaactcctt tttaatttat gtgcaggaaa agtctcagac aataaacaaa gctccctatt
    13261 cttcaataag aatagggagc cgaaatttct gtaaacttct gaccctctaa aagggtgccc
    13321 aaggtgtata atacaagctg actaagggaa agtcaagtac tcaaatgaat actatccgct
    13381 tacaattttt tctttttcga tatcctttga gaaattattt cttaagtcct ttatgagtgc
    13441 aaaaaaaatt taacccacat aataagcttg atataaacat agctaacgtt aaacctaagc
    13501 aaatcagaaa atcgttactg aaaaatttta agtgaaataa tagataaaaa aataaaacaa
    13561 acattatgat agataatatc ccacctaaca aagaactact ttttcctact tgcagcaaca
    13621 gtttatatag cttatcgtag aaacaatctt tttctttcac ccttatttac ctcctttacg
    13681 taaaaaacgt agtccacagt tctcggattt ttggcatatc ttttctatta aataatatca
    13741 aaaacaaaac aattccacta attttttcaa agatgacctt tttttcaaca aaagtgcctt
    13801 gtagggattc attgaaggta acgaatcaca taagtaaaaa ataaggagat gcgacaaaag
    13861 tcgtgcctcc ttatttctaa ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
    13921 caatatccat caaacatgag aagctgtttt tgaaattatt tcttgtgatt cggagttaaa
    13981 tggtaggctc acaacctctg gataaacggt attcaaaagc cagtttctcg gtattaagcc
    14041 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttaata ctcgaaacta
    14101 aacgtctttt tcaaaacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa
    14161 gttcagccaa ttcaaaaaca agaaaagctg tttttgcttg gctattctta ttacttgttg
    14221 cagggcagct ttttcacaac ctctccacta aacggtgttc aatcagctga ccctcggtat
    14281 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg ttagtttctc taatactcag
    14341 gtcaaaacgc tgatttcaca gcctctaatt cacggtattc ctgttttaat aaagttctag
    14401 atattgttct ctttcccact cggaaacagt ttgtctgaat gccgcccatt cgagacgttt
    14461 tgcttcaaca aaatttgaga agatatgttg tcctaatgcg tcaatcatta cattatcctt
    14521 acgcaattct ttgatagcat tatggatagt tgaaggtaag tcctggatat gtgcagcttc
    14581 tctttcttcc tcatccatta cataaatgtt acggtcaaca gctgctggcg gtgtgatctg
    14641 attacggata ccgtctagtc ctgcttgcaa caatactgcc attgccaagt atgggtttgc
    14701 agatggatca actgaccgca attccaaacg tgtggataaa ccgcgagatt ctggtacacg
    14761 tactaatggc gaacgattac ggccactcca tgcgacataa acaggcgctt cataacctgg
    14821 aacgagacgt ttataagaat tgactgttgg attgcaaacg gctgtgtagg cacgagcatg
    14881 tttcagcaaa ccacctaaga aatgataagc tgtttcactt agttccaatt caccgtcttt
    14941 atcatagaaa acatttcctt catcattgaa caaagacata ttgcaatgca taccagaacc
    15001 attgatccca aataacggtt tcggcatgaa agtcgcgtga agtccatgct tgcgtgcaat
    15061 cgttttgaca acgagcttga aggtttggat attatcacaa gcatctacca catctgcata
    15121 tttaaagtca atttcatgct gtcctggcgc tacttcatga tgtgaagctt ccacttcaaa
    15181 gcctagactt tctaattcca acacgatgtc acggcggcaa ttttcaccta aatctgttgg
    15241 tgcaaaatca aaataaccac ctttatcatt caaggtcgtt gtgatcccac cgtcttcgtc
    15301 taatttaaat aagaagaatt ctggttcagg tcctaaattg aaagaggtaa aaccaaggtc
    15361 gcgcatatct ttcaatgctc gtttcaaatt cccgcgagga tcgccggcaa atggtgtccc
    15421 atccggatta tagatatcac aaattaaacg agcaactttt ccatgagcac tttcccacgg
    15481 gaagatcatc caagtggaga gatctggata caaatacata tcgctttctt caattcgaac
    15541 aaatccttca atcgaagaac cgtcaaacat cattttattt tctaatactt tgtcgatctg
    15601 gctcactgga acttctacat ttttgatcgt acctaagatg tctgtgaaca tcaatcgtaa
    15661 aaatcgtacg ttttcttcct caatgattcg tttgatttct tcacctgtta tctgtttctt
    15721 taccatggta tccctcttcc ttttttaatt tattcagtca aagaattgtc tttgcaatcc
    15781 ttgcgactga taatgacctg ttttgcttaa cccaccttgc gccaaaatct catcacgaaa
    15841 aatttgtcga acgtcatgat ctgtcaacgg cttcttgttt tttgcttctt gttttttcgc
    15901 ctgtgccatt tcgtatacac gtttgatccc agccatattc aatccctctg aaagatagtc
    15961 tttaatctca agcaacatat caatatcatt cagcgaatac atacgtcggt ttccttcgct
    16021 tcgttccgga tgaactaaat cttgttcttc ataatagcga atttgccgag cagacaaatc
    16081 agtcagcttc ataactgtac caattggaaa tacggacata gatcggcgca attccttttc
    16141 tcccatcaga cgtccccttt cgttttataa gttggctaaa gtgtaccaat taaaaaaaat
    16201 attgtcaact gcatatgtta ggttttctaa catgagatct ctatttttta taaaataaca
    16261 aaaaaataag aacacaaaaa aggtatctag ttgattttcg taccagatac cttttatttt
    16321 ttattatact gtactatcct ttattagtgt ttttatt
//
LOCUS       DACJGB010000070        15229 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.072, whole genome shotgun sequence.
ACCESSION   DACJGB010000070 DACJGB010000000
VERSION     DACJGB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.072"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            278..787
                     /locus_tag="IYI34_002308"
     CDS             278..787
                     /locus_tag="IYI34_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0560153.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IYI34_002309"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IYI34_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAR0560154.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IYI34_002310"
     CDS             2322..3302
                     /locus_tag="IYI34_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0560155.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IYI34_002311"
     CDS             3424..4842
                     /locus_tag="IYI34_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAR0560156.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IYI34_002312"
     CDS             4913..6382
                     /locus_tag="IYI34_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560157.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IYI34_002313"
     CDS             6392..6859
                     /locus_tag="IYI34_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAR0560158.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IYI34_002314"
     CDS             6895..7767
                     /locus_tag="IYI34_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR0560159.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IYI34_002315"
     CDS             7848..10382
                     /locus_tag="IYI34_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0560160.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IYI34_002316"
     CDS             10538..11311
                     /locus_tag="IYI34_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0560161.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IYI34_002317"
     CDS             11304..12095
                     /locus_tag="IYI34_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR0560162.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IYI34_002318"
     CDS             12105..12596
                     /locus_tag="IYI34_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0560163.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IYI34_002319"
     CDS             12614..13024
                     /locus_tag="IYI34_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAR0560164.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IYI34_002320"
     CDS             13028..14428
                     /locus_tag="IYI34_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAR0560165.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IYI34_002321"
     CDS             14451..14825
                     /locus_tag="IYI34_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAR0560166.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DACJGB010000071        14991 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.073, whole genome shotgun sequence.
ACCESSION   DACJGB010000071 DACJGB010000000
VERSION     DACJGB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.073"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..608
                     /locus_tag="IYI34_002322"
     CDS             <1..608
                     /locus_tag="IYI34_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560167.1"
                     /translation="ENQLFAGDVLEIVLNDQAGEAAAKGVQNKPSTNDEIGNHNPSDT
                     PVVYHDAPPFAPATKIVVEGGLSFLAPSKLDCGQIKATGLTQEAFGQMDIQEKLWIYD
                     QRETKSPWILTIKLSQPFTTDAGFSMQDVLYFKKAEEYQLVNEEELEIVRGQNSTNVQ
                     TDYSEYLNNGRAFKLVLTKENQIAGNYSAEMTWTLADAPTE"
     gene            628..1656
                     /locus_tag="IYI34_002323"
     CDS             628..1656
                     /locus_tag="IYI34_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAR0560168.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1653..2411
                     /locus_tag="IYI34_002324"
     CDS             1653..2411
                     /locus_tag="IYI34_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAR0560169.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2497..3237)
                     /locus_tag="IYI34_002325"
     CDS             complement(2497..3237)
                     /locus_tag="IYI34_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560170.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3298..4551
                     /locus_tag="IYI34_002326"
     CDS             3298..4551
                     /locus_tag="IYI34_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAR0560171.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4852..5100
                     /locus_tag="IYI34_002327"
                     /pseudo
     CDS             4852..5100
                     /locus_tag="IYI34_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5222..5548
                     /locus_tag="IYI34_002328"
     CDS             5222..5548
                     /locus_tag="IYI34_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAR0560172.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5616..6329
                     /locus_tag="IYI34_002329"
     CDS             5616..6329
                     /locus_tag="IYI34_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAR0560173.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6639..7491
                     /locus_tag="IYI34_002330"
                     /pseudo
     CDS             6639..7491
                     /locus_tag="IYI34_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            7612..9477
                     /locus_tag="IYI34_002331"
     CDS             7612..9477
                     /locus_tag="IYI34_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560174.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9525..10934
                     /locus_tag="IYI34_002332"
     CDS             9525..10934
                     /locus_tag="IYI34_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAR0560175.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            11042..11743
                     /locus_tag="IYI34_002333"
     CDS             11042..11743
                     /locus_tag="IYI34_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0560176.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11902..12381
                     /locus_tag="IYI34_002334"
                     /pseudo
     CDS             11902..12381
                     /locus_tag="IYI34_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            13229..13804
                     /locus_tag="IYI34_002335"
     CDS             13229..13804
                     /locus_tag="IYI34_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAR0560177.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13804..14244
                     /locus_tag="IYI34_002336"
     CDS             13804..14244
                     /locus_tag="IYI34_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAR0560178.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14260..14961
                     /locus_tag="IYI34_002337"
     CDS             14260..14961
                     /locus_tag="IYI34_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAR0560179.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5065 a         2485 c         3161 g         4280 t
ORIGIN      
        1 cagaaaatca actttttgca ggagatgtat tagaaattgt attaaacgac caagcaggag
       61 aagctgctgc taagggtgtg cagaacaagc caagtaccaa cgatgaaatt gggaatcaca
      121 atccaagtga tacgccagtt gtttatcatg atgccccgcc ttttgcacct gctacgaaga
      181 ttgtagtaga aggtggtctc tcatttcttg ccccttccaa actcgattgt ggtcagatca
      241 aagcaactgg gttgacgcaa gaagcatttg gccaaatgga tatacaggaa aaattatgga
      301 tttatgatca gcgagaaaca aaaagtccat ggatcttaac gataaaatta tctcagcctt
      361 tcacaaccga tgcaggattt tctatgcagg atgtcctata tttcaagaaa gctgaggagt
      421 atcaattagt gaatgaagaa gaactggaaa ttgtcagagg acaaaattca acgaatgtgc
      481 aaacagatta ttcagaatat ttaaataatg gtcgggcgtt taaacttgtc ttaaccaaag
      541 aaaatcagat agctggtaat tactcagcag agatgacatg gacattagca gatgcgccga
      601 cagaataaag atcaaggaga atgattgatg aatcgaatat ggaataaaag tcatcgaaac
      661 aactgctctt tacttctgat ccttctcctt ggattcatcc tttggcagac acctgtccat
      721 gcctccgaat tcaattttgc ggtagatttt gaattaccgg ctaatcagat tgataagaac
      781 aaaacatata taaatgtaag gatggagcct gggaaaacac aggaaatccc ctacacggtc
      841 tttaaccgaa cagataaatc agtaacagtt gagacattga tccactccgc tacgacgaat
      901 tcaaacggtg tgatcgaata tggagaatca aaaagtaaat ttgatgaaac attaagttat
      961 gatatgaaaa acttagcaaa aacagtggga gaaataactg tccctcctca aagtgcggtc
     1021 actgagacac tttcactgaa agctccagaa aaagagctgg ctggtgtggt cgcaggagga
     1081 atcacgtttc aagaaaaaaa agagaacgtt tctaacaaaa gtacagttgg tgtcgtcaat
     1141 cagttttctc aagtgaaagc aatcttgatt cgaaatagcg aaaaagagtc ttccccaaat
     1201 ttgaaattaa aaaaaactta tgcggatcaa agcgatggaa gaaatgtgat taaggcgaat
     1261 cttcaaaatc caaatgcttc ttatatgttc ggcttgacta catcggttga aatctacaaa
     1321 aaagaaacaa aagagcctta tctttcaata aaaaaagaaa aaatagatgt agcacccaat
     1381 acaaattttg atttacttgt cccattagaa ggaaaaaaat tagctccggg acattatcaa
     1441 gcggttgtga agggtgcttg gaaagaaaaa cgctggaatc tccataccga ttttgaaata
     1501 actgatgatg cagcggacca attgaatgaa aaggatattg atttaaaaaa tcagccagaa
     1561 acatttgact ggcgatggct gatacttgtt ggagtcattc ttattggctt atttgctgga
     1621 ttatttattt taaaagaaag aaggaaaaag aaatgaaaaa aacaattgca ttaagtaccg
     1681 ttctagctgg aagtctatta ttatgtacac aaagtgtttt tgctgagaat aagagctcag
     1741 ccactacaga aggaacgatt tctgtaacaa aaccagatgc cgcaaatcct gaagacgttg
     1801 ttccagttcc tcctgttgat ccaaatgatc ccgatacgcc gtttacacct aacgaaccaa
     1861 atcagggaac atccggatta ctgacgattg accaagctcc agatcttgat tttggaacaa
     1921 tcaagttagg tacagcaaaa acagtatatg ctgcattaca aaacggaacg gacagtacag
     1981 ggacagcaac acaagtacag aatttcgtac aagtaacgga taaaagcgga atttatgccg
     2041 gttggaaact atcagtcaaa cgaacagaat ttattggtac aacatctgat acgaataaac
     2101 taactggttc aaaggtgaaa ttcaaaaacg ccgtgattcg aaatagcgca actaatagca
     2161 gtggaacgcc atctactatt tctggtactg gcgctgaagg aattgagatc ccagcagatc
     2221 aggcagtaga tctagtaact gcaggagcaa atgaagggat tggcacatgg atttattcat
     2281 taggagagaa tgtggaacaa gggagacaat cagtggaatt atttgttcct gtatccaatt
     2341 atgccgtaga caactacgtt tcaacattca catggtcgtt aacagatgct cctacagaag
     2401 ggaaagcgta aaatcaaatc aatgacttca gggagtggag ccgattcaag gccaactccc
     2461 tgaaatctgc tatgcttacg tctcagctct tattcatcaa ctcgaagcag ttttttggaa
     2521 gtcatggaaa cgttcctttg tcttttcaaa gatctttcga ccaacgagta aggagtcacc
     2581 attgaaaaat tctaacacat aatgagcaga atcaattttc tgcaattgcg ataagttgat
     2641 acagtaagat tttaatagta aaaagtaagc atacttttcc aactctgtct tcaattgttt
     2701 taaagaagta ttcactaatt tatcttctgt agttgttttg accataactt ggtttttcaa
     2761 acctttgata ctttcaatat ataaaatttc ccgaggatac aggacgagac ttccaccttt
     2821 ttgatcaata aagattttat cgacatttac gatttgctgt tctttccaat tgatctgttc
     2881 tagcacttga cgaatttgtt cagataaatt cctgtctttt ctgatatatc caatcggaag
     2941 gattttccga ttgatgatct ctactgcaaa attcgtataa gcggtgaaaa aaacaatatt
     3001 actatgtgca ttttttttac gtatttgttc ggcgacatca atacctgtga gtgattgctt
     3061 caagtcgata tcgataaaga aaacatcgtg attatcaata gtacccaact ggatcaactg
     3121 attgatttga gagatagaag tgagctgcac gatattctcg atatgatgat tgaccaattc
     3181 tttgatgatc cattcgctgt gaaactggtt atcttctatg ataaaatact ttttcatatg
     3241 caggctccta ttaaaaaaat atgtgtacat tttgaaacaa aacaatggag gaataagatg
     3301 gtcgcaacag tgacacttta tttgaccttt actttgattt tagatttagg tatctattta
     3361 ttcttttcga aacaactgct agtaaaaaac agctttggtt ttatagcttt gatggtggtg
     3421 acacttttat ttcctatccc taaagtgagc tttccttacc tagttggctt attattgatg
     3481 tttctttttt attttactac gaaaaacctg tttatcagct gtttgatcag taatcttttt
     3541 tttgctatca ctggactcag cttttttctg gtttttgatc tgatggatta tttaggaaag
     3601 agacatcttg gaacagcaag tttttttgca ctacttatcg catttatttt ctatctatta
     3661 gtaaaaagta tagacaaaca gacgagattt ctcaacttgc tttttactta tccaactgaa
     3721 cactattttg gaggtatttg tattttatta agctggggcg tgatttactt ggtattggga
     3781 agtgttcact tccgctcatc taattatctg cttctatctc ttttgtcgat agtcatcagt
     3841 tttttttctt tgtttttttc cattatgttg atcagtttcc aagtaaaaga aagacaacat
     3901 caggaatcgc ttcaactgta tcaaataaat gaagaatatt accatcatct agaggaattc
     3961 aaacatgatt ataaaagtct actgttttca ttaaaaatta ctcttcagga aaataaaaaa
     4021 gaaacgttgg aatttttaga aatcttagaa gatcattctg cagctatttt tgaaaaaccg
     4081 caaatacaac agctaacaaa tattgtatcc ccagctgtca ggggtgtctt tttaaaattt
     4141 ctagaaaaag cagaatcgga gaaaatcccg atccaagtag ttattcctgt cgaagtgaaa
     4201 acaatcccga ttgatctggt tgtttttatt cgctgtttat ctatttttat ttctaacgca
     4261 ttcgatcatc gtgtagccga tcagagtccg attacgatca atctgctgga agaaaaagag
     4321 ggattacgtt ttagtatagc gaacaaggca aatccgacga atttgacgct atcggagatg
     4381 gtcaaaagag gaaaaacctc taaaaaaaat ggcgggttag gtctatacag tgcgaaaaaa
     4441 atgttggatg tttacgaaaa tgcagaacta aaaatcgaat tttcaaaaca gaatcactat
     4501 ttttttgtag aaatgtatct ggcatctaga aagagcaaat cagcctactg acttactgtc
     4561 aaaggataag cttgaggaaa gtctacacgg agatagagac aacaacacag catattcaaa
     4621 agaaacagaa gcgtgatgac cgttgtcacc cttctgtttc ttttgctatg tagttaaaca
     4681 aagtagttga cacttccgct acttagtatt agtacgaaag tatacttcat gatactaagt
     4741 agataagaag tgaaaagcgg aacccacgat cgtcaaaatg ctaactgctt tgcttacacc
     4801 agataaaggg aagagcaata tcgcttattt taggcgtgga gaagcaagaa aatccctacg
     4861 atcacacaaa aaatgctgac ccagcagtta agagagttag aacaagatgg cattgtccat
     4921 cgaaaagtct ataaccaagt cccgcctaaa gtagagtact cgctcaccga agaaggaaaa
     4981 tcattaagag agattctagt agcgatgtct gtttggggag aagaacgtat cgaaaaacaa
     5041 aaagcctcag gggaagaagt cacattactg catacacatg gtggctacct aaattactga
     5101 tttctatgac atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat
     5161 tttttatgta aaaaaatggt atgattaatc aagtaagaaa tgattggaag aaggcaagca
     5221 aatgaatagc atggaaaaag ttaaccagtt tagagatgaa cgtaattggc gacaatttca
     5281 taacgaaaaa gatttggcta tttctatttc tttggaagca tctgaattgt tagaactttt
     5341 tcaatggaaa acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact
     5401 ggcggatgta ttgatctatt catacatgat ggcagacaac cttcagctgg atctagatga
     5461 gatcattgaa gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg
     5521 tgtacgaaaa aaatacacag agttttagaa atgtgtcatt cggctgttgg aaagagcaaa
     5581 agaaaaacag taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa
     5641 gatggctagt caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca
     5701 atgaacagat caaggattat cttgtcagag agacaggaga taaatatgct atagcgaatg
     5761 taacaaaaga agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg
     5821 tagaaccaat gacgacagaa ggggtcatga ggtcgcagat gagaggaaca gatctaccag
     5881 tgattgctag tatcgcagtc ccttcagttt caatcaattt accgattttc aaaggcttgg
     5941 ataatacgtc actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag
     6001 gaaattatgc cttagcaagc catcgtgcaa cgaaccctga attgttgttt acaccgctgg
     6061 aaaatttaga gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca
     6121 aaacattttt taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg
     6181 aaaaagaaat tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg
     6241 tacaaggaga actggaaagt gttacaccga taaaagatgc gacagatgat atgagatctg
     6301 ctttcaatct agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa
     6361 aacagccaaa ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agaggaacgc
     6421 aaagaggtgc atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat
     6481 tttttatatg aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga
     6541 aatggattgt tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat
     6601 tctaggtttt tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga
     6661 ataacaatgt cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag
     6721 gactagcttt tcaaaaaaaa gattggggac gagatttcgc cagaagcggt ccaaaaagaa
     6781 tttattttaa gagattcttt tgctaaaaac caattccagc agctgatggc tgatattccg
     6841 gcagaggaga tcgaatgggt caaacaagta gtcgaactgg ctgaagataa actaaaagtt
     6901 gaattttcgc ctaatattta tctgacattg accgatcatc ttcattatgc gatcacacga
     6961 gcaaaagaaa atatccaact gcctaatcca ttattatttg aaacaaaaaa attttatccg
     7021 aaagaatatc aagcagcgaa agaggctctt ttgttcgtga aacaaaagac gggtgtggaa
     7081 ctttctgagg atgaagccgg ttttattgcc tttcattttg tgaatagcca acaaggaaac
     7141 gaagacatgc aagtgacgat gaccgctacc acaatggtaa gagatatact gagtatcatc
     7201 agtaaattct tcggcatcgt gttcgatgaa gaatctttga attatcagcg tatcattact
     7261 catctgcagt tttttgccca gcgttatctc aaaggggaag gatctgacga gcaagatgaa
     7321 tttctctacg cattagttca aggaaagtat ccaaaagcgt ttcattgtgt cgaacggatc
     7381 aatgagtatc ttcgacaaac gcaaaaacag gaaatgggga tcgcagagca gatttattta
     7441 acgattcata ttcaaagagt agtaagtgag aaaaaaagca tccaatcata aggattgtta
     7501 ctgtatggca ggcaaaacct ggattgaaaa aataggttca cgaatgtttg tgactgtttt
     7561 tttcaatctg ggtttttatt ttttaaataa aaaagaaaga aggaaaaaac aatggataat
     7621 caagcagttg gtcgtcgtgt atgggaagcg gtaggaggac aaaagaatgt caaaagttta
     7681 gtacattgtg ctacgcggtt gcgattcaga ttaaaagatg aatctttagc agatacgcaa
     7741 aagttaaaag aagacccgga cgtgatccaa gtcgtacaaa gtggcggtca gtatcaagta
     7801 gtgattggca gtaatgtagc agatgtttat caatcgatcg tggatgagga aggtcttgca
     7861 gtttcggagg atggaaaaga agagtccaaa aatattttga atcgtctgat cgatattatc
     7921 tctagtatat ttacaccttt tctaggggcg atggcagccg ccggggtatt gaaaggattc
     7981 ttatcattgg caacggtgat gggctggctg acagctgata gcggtactta tcaaatattg
     8041 tttgctgcag cagatggtgt ctttaccttc ttacctgtca tgcttgcttt tacagcagct
     8101 aaaaagttca aagccaacca gtttttagca gtagcaattg ctatggcact tgtgtatcca
     8161 gctatcacag cagttgctgg tgcgggagaa gctatcagtt tctttgggat cccagtgatt
     8221 ctttcaccat ctggttatac ttcttctgtc ataccaatca ttctagccgt atgggttcaa
     8281 agtaaattag agccattcgt aaaaaaagtg attccgcaat ttctgcaaat gattttagtt
     8341 ccgcttgtcg ttttagtagt gatggttcca ttaacatttc ttgctttagg accaatcggg
     8401 acggttgccg gtaatgcttt aggcggtctc ttcaactcga tttatggatt tagtccaatc
     8461 gtagcaggtc taatcatggg aagtttatgg caagtattcg tgatgttcgg catgcattgg
     8521 ggattcgtac cgatcatgtt tttgaatatc gaacaatatg ggtttgatgt attgatgcca
     8581 atgcttcttc cagccatctt ggcacaaggt ggggctgctt tagctgtagc attgcgaaca
     8641 aaagatacga aacttcgagc attagggatt tcttctacag tgacttcgtt atttggaatc
     8701 actgaaccaa ctgtgtatgg tgtgacatta cctttgaaga aaccattcat tgccgcttgt
     8761 atttctggag gtattggtgg agctattatc ggattttccg gagtaaaagc tttctccagc
     8821 agtttggtca gcttattgac gattccgact ttcattaata cggtggatgg tgtagagtca
     8881 aatgtgacag tagcagtaat agctacagga attgcttttg ttcttgcatt cgtaggtaca
     8941 ttgattttag gatttgacga acaaacacaa gacaatcaat tagaaaacaa acatgcgaat
     9001 gcaggagaac cgattacttc agcacggcat accttaaaaa gtcctttaac gggtaaagta
     9061 ctgccattga gtgaagtacc agaccaagtt ttttcttctg gcgtgatggg aaaaggtatc
     9121 gctattgatc cagaagtagg agaactagtt gcacctgcag acggagagat cacgactatt
     9181 tttccaactg gacatgctgt agggatcaca acgactgacg gagcggagat tttgattcat
     9241 atcgggatgg atacagtaga attaaatggg aatggctttg agatcttagt caaacaaggg
     9301 gatctcgtca aagcaggaga tttattgatt cgttttgaca tcgaagcaat tagagcagct
     9361 ggctatagcg taatcactcc agttgtcatt acgaatactg atgcatttgc cgatatactt
     9421 gaattagacc aaaaagagat tattgcaaac gaagatgttc tggcaattgt aaaataagga
     9481 ggaaatcagg atgcaatcac gtttttcaga aaaattttta tggggtggag caactgctgc
     9541 caaatcaatt agaaggggct tatgatcgag acggaaaagg actatccgtt gcagatgcta
     9601 tgcctggagg taagcagcgc tttgcgatta tcggcagtga agaatttgac tggacgattg
     9661 atcaagaaaa atatatttac ccgaatcatc gaggaatcga tcattacgat cgtttcaaag
     9721 aagatcttgc cttgtttgcg gaaatgggat tcaaatgtta tcgtttttcg attgcctgga
     9781 cacgaatttt tccaaatgga gacgaaggta ctcctaacga agcgggatta gaattttatg
     9841 atcagctgat tgatgagtgc ttaaaatatg atattgaacc tgtgatcacg atttctcatt
     9901 acgaaatgcc tcttcatttg gcaaaagaat acggtggctg gaaaaatcga aaactgatcg
     9961 acttctatga acgatttgct cagacagtat tagagagata cagcagtaaa gtcaaatatt
    10021 ggatgacttt caatgaaatc aattcggcct ttcactttcc agctttaagt caaggattag
    10081 taaaaagcaa tggagcagga gagtaccaaa atattttcca agcatggcat aatcaattcg
    10141 ttgcaagtag caaagccgta aaaatcggcc acgaattgcg ttcggatatc caaatcggct
    10201 gcatgattat ctatgcaaca acttacagca tcgatgccaa tccagtcaat caggcagcaa
    10261 caatgatcca aaaccaagaa ttcaattttt tctgcacgga tgtccaagta cgtggagaat
    10321 accctgcata cacagcgcgt acccataaaa aatatggagt cgatccggaa aaattagagc
    10381 aaacggaaaa agacttccag ttgctaaaag aatatccagt ggactacatc ggatttagtt
    10441 attatatgtc tacggcaatc aatgagactg atccacaagc agcaacttca gaagggaact
    10501 tgctgggagg agtaaaaaat ccgtttcttg aggcaagtga atggggctgg cagatcgatc
    10561 cagaagggct gagaatcgca ttgaatgaat tatacaatcg ttaccagaaa ccattattca
    10621 ttgttgaaaa cggtcttgga gccattgatc atatggaaga ggatggcacg attattgatg
    10681 attatcggat cgattattta cgtcgtcata tcgaagcgat ggctaatgca gtagcagacg
    10741 gtgtagattt gatgggctat acgccttggg gatgtatcga tcttgtcagt gcatcaacag
    10801 gagaaatgag taaacgttac ggctttatct atgtggatct agacgatgaa ggaaatggga
    10861 cactgaatcg atcaaagaaa aaatcttttg attggtataa agaagtaatt gcctcaaatg
    10921 gcgtaaactt gtaattaaag cagctgaatg aaaaagaaca cacttctcac atgtgatttg
    10981 tgttcttttt ttatttttta tgtgctattc ttttagtaat agtaaagcaa agggtgacgt
    11041 tatgaaaagg acacgtgtac tatacttgga agtcgcagat aaaattaaag aagatatttt
    11101 ctcaggaaaa tatccagtcg gttcgatgtt gccgacagaa agtgaattag aagaactgtt
    11161 tcaagtcagc aaaatcactg tccgaaaggc aatcgaactg ctggcatcag atgaatatgt
    11221 cgcaaagaaa agtggtcgtg ggacgacggt tctaagcaat cgtccttaca atcgattatc
    11281 caaagcggca agctttacgc aaatcttgga gaactcccga tatgaagtcc ggaaagaaac
    11341 gctggatttg caattggtcc atataaagaa ggaagatcct ttgttttcct ttttcggtga
    11401 gcaagctgtt tgttttaagc gcttatatta tttgaacaat cagccttata tttattttgt
    11461 tcattatctt ccacaagaaa tgatgcaatt cagcagagaa gaatttggca aagaatcttt
    11521 gtatcgtttg ctgacgaaaa aaggatatac tattgactca ttttctgatg atttctcggc
    11581 agtattttta acagaagaag aacaaggaat tctgaaaaca gacgcacaga tcggcatcaa
    11641 acggatacgg aaatcgtcta cgcaaattgg ggaaatgatt gaatattcgg aggcggtcta
    11701 ccatacggag ctgcatcctt accatattga ttatgaaaca taaataaaaa aagaggttgt
    11761 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
    11821 ttcgtttggt taggcttatt accgcagata cacggctgtt tctggattac catttattct
    11881 ggataagggg gtgtggcacg attttcagaa ggaatctatg aaccaatcac tgtattgatt
    11941 ttagcttcga tggtaacccc attcggcttg acgatcgctt atttcttagg gaaaatcatt
    12001 cgtaaaaata tactcaatcg tcaagaaatc gatacattga agaccgcttt tcctatgggg
    12061 atctgtcaga tcacagaggg atgtttccct atcgtattga acgacttggc acgcaatgtc
    12121 atagcaacgg gagtcggtgg gacagtaggt ggcggattaa gcatgttttg gggcgcagat
    12181 agccgtattc cggcatcggg aatgtttgca gtcgctacga tgaccagacc atgggcattc
    12241 attggtgcat tgttagctgg ttcttttgtc acaggaataa ccattttatt attgaaaaaa
    12301 ccagtagatc cgaatgcaga aatcatccaa gaagaaaaag aggaagaaga catatcttgg
    12361 gatgatttga caattagtta ggagaggcag aatggattac aagaaaaaag tacaatagat
    12421 gtaccttgag acaggaatgg tatgaaagaa tcagagttag aaagaatcga atatacagat
    12481 tttggtttag gagcgatcga aactgtccag agcatcggca ccctccttga aatgacgatg
    12541 aaggaaaaat gagaaccttt cgttgtcgaa agggtctggt ataccttatg tcgaaggaaa
    12601 ggaaacaaaa aaactttcct gtactattcc aaaaaggaaa gaaagttatt atacagtaaa
    12661 aaaagaaatt attcttcatg ctagagatca gtacacgttc gagcccaata tcaaacacta
    12721 gttccaggca ggcgcaacag gtgcggttat cactgaattt tcttccagca gtgacgattc
    12781 aagtgatatt tttacaaatc cagctattta tcgttaacaa aaagattttt aggagaagct
    12841 gatgaacaaa aatgatcaaa ttttgatcgt ttagttcgtc ggctttttta tttagagaaa
    12901 catcatttat cagtgaaaca taacacgaga acaaaatgat tttttcgtta ataaaagaaa
    12961 caagtatttg ttgtttaaaa taatacacgt tattggcata taggacgaaa tcccagtatc
    13021 ttctataagt aagtcatttc atatcgatga aaacacgtta tttatttttg ttttatctat
    13081 aaaatggctg aaaaatgatt tttttctaaa tactatatat agtggcgtct atcctatata
    13141 ccactaaaaa actacaaaat ctagtctttt attttaaaaa aacacgtgta gaatgaagat
    13201 agacttttga gttagggagt gtttttttat gaaacttact gttttttgcg gttcccgttt
    13261 tggtaataaa gaaagttaca aatttattgc gcaaacgtta ggaaaataca tggcacaaga
    13321 agaaatcgaa ctggtttacg gtggttcata ctccgggatt atgggagtga ttagtaaaac
    13381 agtactggaa aataacggaa aagtcacagg tatctatccg aatgggcttt ttgaaactga
    13441 attgcctgga aaagatgtta ctacatttat tcctacagaa acgatcgatg aaagaaaggt
    13501 attattattt gaaaagggag atgctgtcct cgttttccct ggaggtctag gtacattgga
    13561 ggaattctca caacttcttt cttggatagc aatcggtctg acgcccgaca agccaatcgg
    13621 aatattgaat attggcggtt attacagcgg cttgcaacaa ttattagaaa cttttgcaaa
    13681 agaggatttt atggataaaa aatggttgga tcaagtaatc ttttccaaca atccattgga
    13741 actggtcaat gaattacgag cagtatctat taaaaatcag ttattattga aggaggcaaa
    13801 ataatgagtc aaatatattt agcaggacca tttttttcag aggagcaaat cgatcgagta
    13861 agtcgtatcg aaaaagcgtt ggaggaaaac aaaacagtca caagtttcta ctctccacgt
    13921 catcaccaag aaagcaatta tgaactattc agcgctggat gggcgcagga agtatacgaa
    13981 aaagatatgg aagaactgac aaatgcagaa tttgttgttg ctatccttga ctttgagcat
    14041 caaacaatcg atccaggaac ggcttacgaa ttaggtgtgg caactatgtt gaaaaaaccg
    14101 atgatcattg tacaagaaga gacggtacct acgaacttga tgattacaca aagtctccat
    14161 acgtatttaa aatcagacca agctgtacgt gaatatgatt ttgaaacatt gcctgttgaa
    14221 acttacgtag gcgagtactt atagaagagg aagggttgta tgtcagtgat tttgatttca
    14281 ggaacgattg gtgcaggtaa gagcagtttg acggatatgc ttgcaaaaga gattgattct
    14341 aaaccatttt atgaaaatgt cgaagataac gaagtattgc cgctctttta ttcaaatcct
    14401 gagcagtacg cttttttatt gcaaatcttc tttttgaaca agcgattttt agcaatgaaa
    14461 aatgctttag tgaacgatga caatgtgcta gaccgatcca tctatgaaga tagcctgttg
    14521 ttccatctaa acgctgattt aggtcgggta acggatatcg aagtacagca gtacgatagc
    14581 ttattagata cgatgctaaa cgagttagat gatgttgctc caaaaaaacg gcctgatttg
    14641 atggtccata tcaaagtttc tttggacacg atgctagaac gtatcaaaaa acgtgggcgt
    14701 gactatgaac agctagaaag cgatgagacg ttatatacct attatgaaac gttgaacacc
    14761 cgctataatc aatggtatga agattttgat gtatgtccta aaatacaagt ggatggggat
    14821 aaattcgatt ttgtagaaaa tccagaagat gcaaaaaagg tgatccgtca gatccaagaa
    14881 aaattagctg aattggaagg aaagcctgct ggaaaatatt ttacggataa ccagcatgca
    14941 aaaggtcaag aattaggcta aaaataaaaa accaaacgac gggaaagaaa a
//
LOCUS       DACJGB010000072        14795 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.074, whole genome shotgun sequence.
ACCESSION   DACJGB010000072 DACJGB010000000
VERSION     DACJGB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.074"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            143..400
                     /locus_tag="IYI34_002338"
     CDS             143..400
                     /locus_tag="IYI34_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560180.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1045)
                     /locus_tag="IYI34_002339"
     CDS             complement(632..1045)
                     /locus_tag="IYI34_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAR0560181.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(1414..2106)
                     /locus_tag="IYI34_002340"
     CDS             complement(1414..2106)
                     /locus_tag="IYI34_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR0560182.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            2191..2367
                     /locus_tag="IYI34_002341"
                     /pseudo
     CDS             2191..2367
                     /locus_tag="IYI34_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(2543..3112)
                     /locus_tag="IYI34_002342"
     CDS             complement(2543..3112)
                     /locus_tag="IYI34_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR0560183.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(3186..3509)
                     /locus_tag="IYI34_002343"
     CDS             complement(3186..3509)
                     /locus_tag="IYI34_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560184.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3663..4475)
                     /locus_tag="IYI34_002344"
     CDS             complement(3663..4475)
                     /locus_tag="IYI34_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560185.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4829..5677
                     /locus_tag="IYI34_002345"
     CDS             4829..5677
                     /locus_tag="IYI34_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAR0560186.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5822..6763
                     /locus_tag="IYI34_002346"
     CDS             5822..6763
                     /locus_tag="IYI34_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAR0560187.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6753..8924
                     /locus_tag="IYI34_002347"
                     /pseudo
     CDS             6753..8924
                     /locus_tag="IYI34_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(9036..11207)
                     /gene="copB"
                     /locus_tag="IYI34_002348"
     CDS             complement(9036..11207)
                     /gene="copB"
                     /locus_tag="IYI34_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAR0560188.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(11227..13413)
                     /locus_tag="IYI34_002349"
     CDS             complement(11227..13413)
                     /locus_tag="IYI34_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAR0560189.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="IYI34_002350"
     CDS             complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="IYI34_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAR0560190.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13635..14075)
                     /locus_tag="IYI34_002351"
     CDS             complement(13635..14075)
                     /locus_tag="IYI34_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAR0560191.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(14149..14688)
                     /locus_tag="IYI34_002352"
     CDS             complement(14149..14688)
                     /locus_tag="IYI34_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0560192.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4282 a         2991 c         2564 g         4958 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccctcaaag tgaactgaac cccaaaagtt
     1321 gaactattta atggactgtt tccgatattc tactggagat agtccattaa atagttcaac
     1381 ttttggggtt cagttcaaag agggggggat tttttatcgt tgtggaatat ttaaatacca
     1441 acgcttgtca tgaaaatctt gtgctccacc cttagtattt ccttctggat cgtttgtcgc
     1501 acgcatcatg acgtatactt tcttattagg gaaatcacgc atattgaaag atacatgata
     1561 gccaacattt cctaaagtat tataagcttg atttacgtct ggtctataaa tcccatcagc
     1621 tcttactcta gctagttctt ttccagtatt gtagtccata atgaaaatat actcgtattt
     1681 ataattagca atgtgccatc cagctacatg caagtttgcg ttttcgattt ctccgaactg
     1741 atcaatgtgg gcgtaatttg ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt
     1801 tggatcaatt actggtttat cctccgaagt agttggatta tcatccgtaa aaccatgagc
     1861 caaatcatag gctagttttt ctttacttac acccatttca gaaagataac cgtaaggatc
     1921 tgtatgatcg ccccaaatat tttgtgttac ccataaatgc gatttgattc ctggttggtt
     1981 ataaggagtg tctaatgtta atggaatacc atatttcatt gctgaatctc ttgccaattc
     2041 aacgtatact ttatagttct tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat
     2101 ttgcacagga cttttggctc acgaggttgt cctactctac atcttgctga tagatatgct
     2161 gaaaggaaaa caccaacatt atgttttgct ttttcagcta gaacgagaca agcaggaata
     2221 cctttagaag atatcaaagg ttttttaggt cataaagatg tttccactac acaagtctat
     2281 gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa
     2341 gagcaaataa aaaagcactc aaattgagtg cttttttaag ctcactatca ctaaaactat
     2401 cactaatttt atttataagc gaacatgttt ctatcactga ttttttaaat ctagcgaaaa
     2461 acccttatct taaaggcgtt cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc
     2521 tagaagtgcg aacatggttt tcttatagct catgtagaaa aattttcttg atgatgattt
     2581 tatccatcaa ttgagattga ctgttttgct ctcctatgat ttcagctatg attgtccgat
     2641 tttgcggttt tcttcttaac tggtagttga ttttcccagt ttccatcaac tcgctaagca
     2701 tatccacgac tccgccaaat ccggtctttt tcgtaataaa acctagtgct accattcgtt
     2761 catctagatt ttttgctgta atcgtcacac cagtaatgag cgagtgctgt aatgtttggg
     2821 ccagctcttc catcccttca gcattggata cgacggaagc tgttttgtta ataggttctt
     2881 cgtttatttc ttttggttct ttcctctcca ctttcactgt ttgacgaagg acatctagtt
     2941 cttgcaagag cgtttcgtat tgttcttggg tcagcatcac gccagctact ttttcacgat
     3001 taaatatata tacgccagtt cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc
     3061 gtttcacttc tgtgatcgaa gatgtgggaa cttctaattt ctttaattcc aatggatttc
     3121 ctcctttact aatcgctttt tattatagca cagactgact tgttttgatc ttttttccaa
     3181 aggttttatc ggtttttgcc taaccagtaa ttgatatcgg taagaagtct ttcgatacca
     3241 aatgaaggat ggtacagata cgttttttga tcttcgttat ggtctagtgg aaaaaaatga
     3301 tcgatgatca ctagatctgc ataccgaggc ggcttatttc gagtacatac atgtatagag
     3361 atccgttttc gatgtcttag cgttttgttc agtaccccag caagtcttct tgcttcttgg
     3421 tgttcatagc gaaaaaaaaa gacgattttc acttcaggag ggtggacatc taaataccct
     3481 cgttcctgga gtgttttcag taaaagcatc ccccactcta ctgcggggtg tgcaagtgct
     3541 tgttggatcg ctagccgaaa ttgatatagt ttatctttgg aattgggaaa tttttctaat
     3601 gtttcctgat aataacgaac ttcttctatc agccggtcat ggataccaat tacgtaacga
     3661 agtcattctt ggtaaagtac ttttgtcagc tttgatttgc tttcgctctc aaattcgcta
     3721 ttcagccaaa acagcgaatc aagcttttca gtcacaatcg tcaatttccg atagtcttgt
     3781 gtctcttcta tccgtttcca ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt
     3841 gctagataat ttttttcttt tattggcaaa atctgctcct ctctaaatag caattcagga
     3901 aacaattcaa agaatagtgt tcgactgtct cctaaaatct cgatttctgc cggatccttt
     3961 ctttgcttca ataattggat gaacatctca gaaaaggact gctctccatc atctgcaaaa
     4021 aaatgcggaa aatctttcaa aaagcaaaac agcagatatc ttacttttct ttctgaacca
     4081 aagatctgtg cttttttata agaaaaagaa aggtggtact cgccaaacca ctttctcaat
     4141 cgctcttgcg tagaacgatc gtacttctgc tcgtaaggtt cattcaatag cagatttttc
     4201 agtaaacaaa acgggggtga ttggatcaga aaaacaaaca agagataagg ttcgctatat
     4261 ccttgatcat aaactaactg aacggttttc ttatgaagaa tcagctgaat gggacagtcg
     4321 ctttccgcca aacgtttcag atgccgatcc actgttttaa catcgatttt cagttcttca
     4381 gctaaatcag cttttcgcca gatcaaatct ttcattagat tgattagtga aaataaattc
     4441 tgtgcatttt ttgataatag tttttgccat ctcatcgatc atttctcacc tgatttctaa
     4501 acatattgtt tgatcaaaat gggttcactc ctaaaaagat aaaaaactcc ctttctattt
     4561 atttaaatac gcaaatcttc ctcattattt tttattttac tcaatttaat cgtattctca
     4621 tttcaaaacg aatgtacttt aaaaaaacat aataagatca ctttgcttgt aggatgtatt
     4681 cccttccatt acaatgaaat aaatagcgat tttatgagaa ggtgatgtga aaatggaaga
     4741 aaaaatagaa gaatccaaac aaaaaatcga tcagttgata taagaattag aaaaagcggt
     4801 catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag gtggtcatgt
     4861 cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc
     4921 tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta gtgatattct
     4981 tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt
     5041 taccacgatc ttgttagcag atgagatcaa tcgtacgact cctcgtactc agtcagcact
     5101 tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc ctctagacaa
     5161 tcattttttt gtcttagcta cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc
     5221 ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt cttttgatga
     5281 cgaacttttg ctacttttgg ataaattcga aaaagtgttg gataacttaa atgaagtatt
     5341 gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg tcagtcccga
     5401 agtggcatct tatgccttgc agttagtgtc agcaacaaga agtcatgctg ccatccagct
     5461 tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt atgctttaat
     5521 ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt acgtgttttc
     5581 ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaagaaac aaatcattca
     5641 tactcttgtt gcacaaattc ctattcctgt gaggtagatt gaggtagaca atggaaaaaa
     5701 tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt gtgctttttg
     5761 ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact ctttatgtat
     5821 catggagctt gatttcatta agcagctttc taaagaaact ttcggttact tgtacattgc
     5881 agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca aaaaagagaa
     5941 ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa
     6001 acaagagatt ttgattctta caaataaacc gcaagaagtc caattgcttt ggcagccaaa
     6061 agaaagagga tgccatgaaa cattaactgt catttttaca gcctatgata gtctaggact
     6121 tttcagaaaa agtgcaaaaa gagagatctc ttttcctgtg actgttctgc ctgctttttg
     6181 taagattcaa gcagaaaaat tacagagagt tctggaaaaa aaacagcagc tgaacgcgta
     6241 ccaaagaggt cttgattttc gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg
     6301 aatcgattgg aaattgtctg ctcatactca agagtggtta taccgtgaat acgattatca
     6361 agaagaagaa tgttctcctc ttttttgctt ttggggttcg cccagtccaa aatttgaacc
     6421 tctgctagac ttgtattttt ctttatggca tttgagaaaa gaaaagcagc cggaacttct
     6481 gatattaggc aaccgcgcct ttcatggcaa agaccccggt catacctatt ttgcttcttt
     6541 ggaagctggc gatgagaaag cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat
     6601 cgtcctcttt gttcctgaga attctcctga agtctcagaa gctattgaaa cattgtcaaa
     6661 agatcgaacg gtttatcttg tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga
     6721 caagatatgt tcattgccag gaggtgaatg gattgacgat taaacgggaa aaatggctgc
     6781 gtggcgttta ctcctttgtg atactaagtg taggtgctcc gatatttgta gtagctaatc
     6841 aattgcaaaa cagctacctt cttatcctaa ctattggtgt cctatgcctt attgtctgct
     6901 gtattcctaa aaaatgggta cgtttgctgc tttctttgcc agtaatgata ggttgtttgt
     6961 ttttatattt tccttcgaag acattttcca tattatggat cttacagttt ttttcgtcag
     7021 tcacagagga attcttccat ctatcccaag gtaaacttta ttatttgcca gaaaagacag
     7081 ctttttttct cataatgttg ctgatctatt tatttattac attgaccgtg gattacgact
     7141 attggtacgc accctttctt tctttgatgg cttacttcat gatgcttacc gtttttcgga
     7201 aaaaagattt actgtacgaa atgattgcgg tagtaactgt tttgttcgtg tatctggcag
     7261 caaggcaatt tttctccatc cggttgttgt cgggaaacac tcgattccag tcattgacta
     7321 cagttctgct tatttgtttc ttaactttta gcgctatctt gcctttatat cttccgaaag
     7381 tacaccaatc tctcgaggaa acgagtgaac cgatccgaga atatcttaat gatgaagggc
     7441 tttatgacac actccatgag tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga
     7501 atgatgaaca gcttggtgga cctttatatg ataatggcga agttgttttt acagcgaacc
     7561 aaaaaggaaa tggctattgg aagatcgaaa ataaaaatca ttacaccggt tctggatgga
     7621 tagaagatca ggacgcaccc atccaaccaa tccaagaatt ccctgttgac atcacgagct
     7681 accaaaccgt tatagaagac aaagaaaaca ttacattgac cctaaaaaaa caagttttct
     7741 taccttatcc ttatggaaat actaccttga ctgatttttc attagctagt ggacaaacgc
     7801 tttactattt gcctgaaagc cagcgattca ttcctgtccc gaatgaatcg acacgttctg
     7861 caactttaca aacagaaagg ttagttttta cgagagaaga attaaaaagc agtcagttaa
     7921 aagctgatga tttagaaaat cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac
     7981 gtattgagga cttggcggtc gaattgacca aaaatgaagc aactttaatt cgtaaagtcg
     8041 aagctgtcta acagtattta aaaacaagcg gaaatttccg ctattccaaa acggataccg
     8101 ggcatactcc aaaagaacac gattatgtgg attatttcct ctttgattca aaagtcggct
     8161 attgtgacaa cttttcaact tctatggtcg tccttcttcg aacacttggt atcccagctc
     8221 gctggacaaa aggttttaca aatgggctca gaacaggcac agataatcaa gggaatgagg
     8281 aatacagcat tctcaatagt catgctcatt cttggccaga ggtatacttt cctggatttg
     8341 gctggcttcc atttgaacca accccttcat ttgccaatcc aatgggttca caaccagttg
     8401 ctgaaacaac cgaaaccgtc cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg
     8461 cagaaactat ttccagtgag agatctgagg tcattccatc agaagaaaca acactcacac
     8521 cagcttataa aatcgtctta ttcagccttg gagtgtgttt atccgtcttg ttgttctttc
     8581 tattgttatt cagaaaatct ctgcgctgga caagttatct tatctatctt catttttttg
     8641 cttctactaa tacgattgca tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc
     8701 gcaaacccgc agaatctttg caggaatata gcctgcgaat agaacaagaa ttcaagctag
     8761 atctgcgttt cattcgatta actactcaat atgaagcaga aatatatgga cgacaagcag
     8821 atccggtaaa attcgataaa aaaacctatt tagaaataac gcatataata agaaaaatgg
     8881 tatacaaaaa aattctgaca aaagggagcc gcttcaaaaa ataagataaa atttctggag
     8941 aatctacttc tggaatgccg agaaccagct gattgagtat cgattactcg aaaaaaaggt
     9001 tgtgacacgt tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca
     9061 tttagagcta caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat
     9121 atgataccaa ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct
     9181 gccccccacc agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa
     9241 tgcaaaatat cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata
     9301 tctgtccctg caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg
     9361 atcccatcac ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct
     9421 tctttatcat ctggtaataa accgccataa tattcttcga tccctaaata atctgctact
     9481 gcttgggcag cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc
     9541 aatgttcgga taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca
     9601 gctagatgat tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc
     9661 aataatgctt gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc
     9721 acttgttgtt ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca
     9781 gcttgataag gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga
     9841 tgattggcat tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca
     9901 tcatcaagta cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg
     9961 tccagcatga tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt
    10021 aatccgtttt tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct
    10081 agagcatgag gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga
    10141 tctgtcaaga acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac
    10201 agccattttg ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt
    10261 cgcaccattt ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt
    10321 tcgatcgttc catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg
    10381 attcctcttg attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt
    10441 acaccatctg ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact
    10501 tctttcaatg aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc
    10561 agtaattctg ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc
    10621 cagtgaccta gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg
    10681 tgtgtatgtg gattgagcag atttgcgata aacgaataga cactataaat atatgcaact
    10741 gtaatcccca tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta
    10801 gctccactta aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg
    10861 agccaatcag aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa
    10921 aaaagaatga ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc
    10981 atgtgcatgg aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt
    11041 tgtttgctgt ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga
    11101 ctatggtcat gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact
    11161 acttcttttt tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc
    11221 cttctcttac tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga
    11281 actaaacgcc atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg
    11341 aataccaatc gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt
    11401 caacctagac aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac
    11461 atcggctgtt tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa
    11521 tgctaaggcc ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt
    11581 ctgaagtttt tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt
    11641 tggatcaata ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag
    11701 cataaaaaca tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt
    11761 gatttgatca gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac
    11821 tgtttttccc tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt
    11881 caattcaatt aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct
    11941 gattccagca cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag
    12001 ttttccgtat tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata
    12061 aaaaatagat aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt
    12121 aatcgttcca gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc
    12181 tccgccttta attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt
    12241 tggagtcgct aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat
    12301 caatgcgagt tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat
    12361 gactaatacg atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc
    12421 tttgctccct tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc
    12481 gacttgtgta acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt
    12541 gtcatccgga ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc
    12601 actgcttcct gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag
    12661 ttgatcacca acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac
    12721 ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt
    12781 tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc
    12841 aaaataaaga tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc
    12901 agccgatgtt ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg
    12961 gtacgcacct ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa
    13021 ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc
    13081 aatcattgac aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat
    13141 ttttttcaaa tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa
    13201 aattgcacca tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc
    13261 atatttgaca cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg
    13321 tccgttcagc tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac
    13381 gaacgtatcc attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat
    13441 ccattgattg cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta
    13501 ccgtttgctt ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt
    13561 tcttctactc ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc
    13621 attttttctt cctcctattg atgttgttta cattcacatt gacctgggat acagttacat
    13681 tcgatcgtat ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct
    13741 tgagtcaaag tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag
    13801 atatgggaaa aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga
    13861 taataaatga atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga
    13921 cccagcaaag ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg
    13981 atttcttggg ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa
    14041 tcactgatct ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa
    14101 tgtaatcatt atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt
    14161 gctctctatt ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat
    14221 cagtttattt cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc
    14281 ttcaaaatgc cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa
    14341 tgattcaggc atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac
    14401 tactcggtag atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt
    14461 aatcctttcc tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac
    14521 cataaacaat tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt
    14581 atgccataaa aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc
    14641 gagggtcgat tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca
    14701 cttctctttg gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact
    14761 gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaa
//
LOCUS       DACJGB010000073        14703 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.075, whole genome shotgun sequence.
ACCESSION   DACJGB010000073 DACJGB010000000
VERSION     DACJGB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.075"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(106..1053)
                     /locus_tag="IYI34_002353"
     CDS             complement(106..1053)
                     /locus_tag="IYI34_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAR0560193.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IYI34_002354"
     CDS             1299..1763
                     /locus_tag="IYI34_002354"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAR0560194.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IYI34_002355"
     CDS             complement(1825..2868)
                     /locus_tag="IYI34_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAR0560195.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            3219..4082
                     /locus_tag="IYI34_002356"
     CDS             3219..4082
                     /locus_tag="IYI34_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR0560196.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(4211..5833)
                     /locus_tag="IYI34_002357"
     CDS             complement(4211..5833)
                     /locus_tag="IYI34_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0560197.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            6082..6603
                     /locus_tag="IYI34_002358"
     CDS             6082..6603
                     /locus_tag="IYI34_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560198.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(6789..8519)
                     /gene="spxB"
                     /locus_tag="IYI34_002359"
     CDS             complement(6789..8519)
                     /gene="spxB"
                     /locus_tag="IYI34_002359"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAR0560199.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            8684..9502
                     /locus_tag="IYI34_002360"
     CDS             8684..9502
                     /locus_tag="IYI34_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAR0560200.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(9605..9802)
                     /locus_tag="IYI34_002361"
     CDS             complement(9605..9802)
                     /locus_tag="IYI34_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560201.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            9971..10549
                     /locus_tag="IYI34_002362"
     CDS             9971..10549
                     /locus_tag="IYI34_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAR0560202.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(11594..12097)
                     /locus_tag="IYI34_002363"
     CDS             complement(11594..12097)
                     /locus_tag="IYI34_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAR0560203.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            12456..13481
                     /locus_tag="IYI34_002364"
     CDS             12456..13481
                     /locus_tag="IYI34_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR0560204.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            13804..14682
                     /locus_tag="IYI34_002365"
     CDS             13804..14682
                     /locus_tag="IYI34_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560205.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         4618 a         2592 c         2845 g         4648 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa taaacggtgt
     3001 tccagaagta gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt
     3061 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt
     3121 agaacttgcg aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga
     3181 cgataagggt aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt
     3241 cttgttgtga tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga
     3301 atctccaatt gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc
     3361 gctacatgaa tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc
     3421 gatatttaga atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt
     3481 tttccagtgg attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg
     3541 aagaatatga agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag
     3601 ggcttttagt attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc
     3661 tagagtggct ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga
     3721 aatatctaga acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga
     3781 gtgtaaagcc tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc
     3841 gaggaagaaa caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac
     3901 cacttgatca aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa
     3961 aacggctttt ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa
     4021 cgatcgccag tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat
     4081 aaaagcagta actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact
     4141 cttaaaagat agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct
     4201 aaataggcca ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa
     4261 attcatcata tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc
     4321 ggtttgccaa tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa
     4381 atgccatcaa gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat
     4441 catctaatac aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt
     4501 tctctccacc agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc
     4561 ttcctaagaa gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca
     4621 accagtctaa aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag
     4681 aattacttgt tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga
     4741 taactttgaa tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt
     4801 ctttgctcaa attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta
     4861 cattttctac ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg
     4921 gataacgtct agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac
     4981 gtgaagttgc ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga
     5041 gttctttgat ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt
     5101 gactagattc caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta
     5161 gatccgccat atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca
     5221 cagtattttc aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac
     5281 cgtttgttgg ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata
     5341 acacttttac cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag
     5401 ggatgttcag accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct
     5461 cagcaaattc tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt
     5521 tcatgtaaat cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga
     5581 ctgtttcaag tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc
     5641 gttcatctgg tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt
     5701 taaggaacgt tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg
     5761 taaatttaat attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat
     5821 ttacagtaat caattttttt cctcactttt atcattatta gttttgacaa gtcttataga
     5881 attatatagg ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga
     5941 aacagaatga aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta
     6001 gtttgcaaca gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa
     6061 gaaatgaaag aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt
     6121 ttgggaattt tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta
     6181 gttgcaattg tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt
     6241 gtacggaaca gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg
     6301 atcatcgata ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca
     6361 ttgccttttg tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct
     6421 gacttggcaa gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta
     6481 ggaattttat taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc
     6541 tttttagttg gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga
     6601 taagtaaaat caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg
     6661 gaagccggct tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa
     6721 atttatgcaa aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag
     6781 cgcagttgtc acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca
     6841 tatttttctt tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga
     6901 tcttctactg gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct
     6961 gattttgcag ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta
     7021 tttgccccga gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat
     7081 ttcttttgtt ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag
     7141 acaacattaa taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata
     7201 gcaaacccgc catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg
     7261 gcaattcctc ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt
     7321 tgtttaccgt tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg
     7381 acgaaaatcg catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga
     7441 atcggttctt cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt
     7501 tcttgatttg ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt
     7561 tgcaaagctg tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat
     7621 tttgacgcat cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca
     7681 aatgggaagt cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg
     7741 ggtttagtag cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt
     7801 ccttttgcta gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt
     7861 tgcggaaatc cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca
     7921 agataaggca aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga
     7981 gtgtgagcgg tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata
     8041 gtcacgatag ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc
     8101 ggaagacttt gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga
     8161 ttttcattta gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag
     8221 aacgctacta gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc
     8281 gcaccaggtc ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca
     8341 gcggctgcga gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt
     8401 tcttgatata gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg
     8461 tcgatacccc agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg
     8521 gtgtttcact cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt
     8581 tttatcgaaa atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat
     8641 atgctagagt agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt
     8701 gagacttgaa ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc
     8761 tcttggtgcc gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat
     8821 gttaggattt gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc
     8881 tattacaaaa gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg
     8941 tttaggcggt gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc
     9001 acaacatgaa gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc
     9061 tattacattg cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc
     9121 aactgcggat aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg
     9181 tatccaaaac ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag
     9241 tcgtaaaaag gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt
     9301 gattggagcg ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc
     9361 cgcaggagca atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt
     9421 gtccagcaaa catcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat
     9481 tttggatgtg gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg
     9541 aacttatatc gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca
     9601 tacattaata atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga
     9661 taagtgcgat tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga
     9721 caaaaagact gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta
     9781 agaacatatt tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa
     9841 agtataacag ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac
     9901 ttaaatgatc aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga
     9961 ggagaatatc atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat
    10021 catgactatt ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc
    10081 tgcattaggt taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg
    10141 gaacaatcat catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc
    10201 gaacagtttc tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga
    10261 gtacatcaat agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat
    10321 tgcgtattat atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt
    10381 gaaggcgatg ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg
    10441 aattgtctta gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct
    10501 agctgcttgg atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg
    10561 tttaaaattt aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata
    10621 ctagtattca aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta
    10681 gttttgtcac gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg
    10741 tcgtaggatt tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga
    10801 tgttctatct atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt
    10861 ttatttgtgg aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc
    10921 cacagtggat aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga
    10981 tttcttttga taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt
    11041 ataaaacggt gtggtttttg gaataaaaaa ataacatatc tcattttaca cttttgttat
    11101 tttatactta aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct
    11161 aaaaactact gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac
    11221 tggcagaatc aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta
    11281 attacttata aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa
    11341 gacggaattc ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt
    11401 taagctaaaa aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact
    11461 gcaagtcgtc ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc
    11521 aatgactgaa taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt
    11581 tttacgaacg attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca
    11641 gaaataagaa taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata
    11701 gatgtttgtt tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta
    11761 tcaaatcagt gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga
    11821 agcttacaag gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat
    11881 tcaaaagcac cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg
    11941 tcgtattttc aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag
    12001 tgagaaattg tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata
    12061 aataaatccc taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag
    12121 tataccatcg aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag
    12181 ttatcgtacg aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta
    12241 agatagcgag ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt
    12301 cgttttttgt tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc
    12361 aagaaatagt tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc
    12421 agcttttttt ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca
    12481 ataaaataca agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat
    12541 tatggttagg ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct
    12601 ttactttttt ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga
    12661 tcggttgcgg actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc
    12721 ttgaaccatt tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc
    12781 atatttctaa accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc
    12841 agctgccaat cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg
    12901 ccctgattgc ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa
    12961 aagcaacttt aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct
    13021 acagcgctgc cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt
    13081 attggttgac cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc
    13141 atcgacaagt ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt
    13201 catttgtgcc gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg
    13261 cacaatttct agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt
    13321 tgatgctatc tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt
    13381 tagggatcgt cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt
    13441 tgcaacatta tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc
    13501 aaaagaggtt atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga
    13561 ttctcagcat taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta
    13621 tatttattct atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg
    13681 tcaatgcttt ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat
    13741 agttagtgta caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa
    13801 acaatgaggt tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc
    13861 caaattctgg cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa
    13921 gcaaaaaaca tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat
    13981 aaagcaaagg cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca
    14041 ttagctattt atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag
    14101 attccaagaa ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat
    14161 ttccaagtaa caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt
    14221 ggcttgatct ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac
    14281 acggatctaa gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat
    14341 ttatctcaga ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat
    14401 tttcctgatt accattacca agtgatgtac acagatactt ggttactatc acccaatctt
    14461 actaaatggt tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta
    14521 tcagttgatg aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa
    14581 atacatgatt atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca
    14641 ggcgagcaca ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata
    14701 gtt
//
LOCUS       DACJGB010000074        14412 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.076, whole genome shotgun sequence.
ACCESSION   DACJGB010000074 DACJGB010000000
VERSION     DACJGB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.076"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            59..174
                     /gene="rrf"
                     /locus_tag="IYI34_002366"
     rRNA            59..174
                     /gene="rrf"
                     /locus_tag="IYI34_002366"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(227..697)
                     /locus_tag="IYI34_002367"
     CDS             complement(227..697)
                     /locus_tag="IYI34_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAR0560206.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(856..1314)
                     /locus_tag="IYI34_002368"
     CDS             complement(856..1314)
                     /locus_tag="IYI34_002368"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAR0560207.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1485..2630)
                     /locus_tag="IYI34_002369"
     CDS             complement(1485..2630)
                     /locus_tag="IYI34_002369"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAR0560208.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2643..3560)
                     /locus_tag="IYI34_002370"
     CDS             complement(2643..3560)
                     /locus_tag="IYI34_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAR0560209.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3988..4551
                     /gene="efp"
                     /locus_tag="IYI34_002371"
     CDS             3988..4551
                     /gene="efp"
                     /locus_tag="IYI34_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAR0560210.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            5037..6296
                     /gene="lysA"
                     /locus_tag="IYI34_002372"
     CDS             5037..6296
                     /gene="lysA"
                     /locus_tag="IYI34_002372"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAR0560211.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6283..7083
                     /gene="dapF"
                     /locus_tag="IYI34_002373"
     CDS             6283..7083
                     /gene="dapF"
                     /locus_tag="IYI34_002373"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAR0560212.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7086..7967
                     /locus_tag="IYI34_002374"
     CDS             7086..7967
                     /locus_tag="IYI34_002374"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAR0560213.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7964..8680
                     /locus_tag="IYI34_002375"
     CDS             7964..8680
                     /locus_tag="IYI34_002375"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAR0560214.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8686..9381
                     /gene="dapD"
                     /locus_tag="IYI34_002376"
     CDS             8686..9381
                     /gene="dapD"
                     /locus_tag="IYI34_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAR0560215.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9397..10398
                     /locus_tag="IYI34_002377"
     CDS             9397..10398
                     /locus_tag="IYI34_002377"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAR0560216.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10395..11756
                     /locus_tag="IYI34_002378"
     CDS             10395..11756
                     /locus_tag="IYI34_002378"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAR0560217.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11716..12867
                     /locus_tag="IYI34_002379"
     CDS             11716..12867
                     /locus_tag="IYI34_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAR0560218.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12842..14011
                     /locus_tag="IYI34_002380"
     CDS             12842..14011
                     /locus_tag="IYI34_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR0560219.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14186..>14412
                     /locus_tag="IYI34_002381"
     CDS             14186..>14412
                     /locus_tag="IYI34_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR0560220.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNV"
BASE COUNT         4661 a         2547 c         3072 g         4132 t
ORIGIN      
        1 tgacttcaaa tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg
       61 tggtggcgat agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct
      121 tagcgccgat tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc
      181 aaaaagatga actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt
      241 caattgcagc ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc
      301 catttctatc aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag
      361 gagcttcatt tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca
      421 attgatgcat agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg
      481 ctgaatctag ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg
      541 tctgataagc ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg
      601 ttgcagtgtt aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc
      661 cgtcttctaa agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta
      721 tataataaaa agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt
      781 tcagacaata aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt
      841 acgttctttt agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc
      901 acgagccaaa gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc
      961 attgcttgca ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc
     1021 taaaatgtct tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc
     1081 cattggagac cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg
     1141 ctcaagtgaa tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac
     1201 gtcgtattta atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata
     1261 aggtgctttg acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa
     1321 gactcctatc tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa
     1381 tttacaaaaa cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct
     1441 gaaatatttt ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg
     1501 tcaaatgcct gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga
     1561 cggatcaagc cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga
     1621 gtcatgacag aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta
     1681 aatagttgga gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa
     1741 atcattccgc taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt
     1801 aatcctggat agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca
     1861 aaagcatttt tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt
     1921 aaccagctat cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga
     1981 tcggctagat ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct
     2041 agatatttgg taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa
     2101 taaggagtag caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg
     2161 gcactttttt ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt
     2221 gcttttgtat tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc
     2281 gtgtactcga gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag
     2341 acatcatctc ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc
     2401 ccagaaagac cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt
     2461 tcttccaatg caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg
     2521 ccgacgccat cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt
     2581 gtgggatctt cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc
     2641 tcctataagt aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg
     2701 acacgatgac caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca
     2761 ctcgaacttc caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct
     2821 tctacatcag agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg
     2881 aattctacac cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag
     2941 ccctctggtt ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa
     3001 gttccagcaa aggttccacc actcccaatt cctgctacaa aactatggat attttctttg
     3061 atttcatgaa agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt
     3121 tcaaattgaa gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg
     3181 atggcaccga tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc
     3241 atcaattgtt gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg
     3301 tatgtgagtg cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc
     3361 gtcgtttttt cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat
     3421 ctatctttga tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct
     3481 ttaggcatag aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct
     3541 gtaattgtcg agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac
     3601 tattaaatca gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac
     3661 ctggtactca ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg
     3721 caccatacaa cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc
     3781 aagtaaaatt tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa
     3841 aaaatatttc gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc
     3901 ttaaaagata gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta
     3961 tgataaagaa aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg
     4021 actttcgtac aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt
     4081 aaaggaaata cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac
     4141 acaacgttca gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa
     4201 tatttgtaca cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat
     4261 gaaattcctg tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta
     4321 aaaatccaat ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta
     4381 cgtgttgtgg aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca
     4441 gcgacaatgg aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg
     4501 ttggaaatca atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac
     4561 ggattgataa gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc
     4621 ctttcaagag gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat
     4681 ttatattttg aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga
     4741 agaaattgaa atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc
     4801 gaaaatattt tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc
     4861 taatagttcg ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt
     4921 cgaaatatac tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc
     4981 tttatcaaat gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga
     5041 cagaattgat aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc
     5101 tgtatgtgta tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg
     5161 tatcgaaacg ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga
     5221 tgctgcattt agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct
     5281 ataccgcttt gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga
     5341 cgccagagga actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt
     5401 tgatggaagt tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atctgtatca
     5461 tgcttcggtt gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata
     5521 ttgattcaaa attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga
     5581 taaaagaaag cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt
     5641 ttgatgttac tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg
     5701 accaacctct ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc
     5761 agccgatccc agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg
     5821 aaaaacgaaa actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag
     5881 aagctggtac aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt
     5941 actttgtaga tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg
     6001 attgtgattt agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa
     6061 aaatgtgtga atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg
     6121 gtgatctttt agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca
     6181 atcgagcgac acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc
     6241 gcagacaagc attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat
     6301 aatggctgcg gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa
     6361 ttagccgctt ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa
     6421 atcgatccgt tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga
     6481 aatggcattc gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa
     6541 ttcaacgtag agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt
     6601 tattgcagcg tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa
     6661 acttatccaa taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt
     6721 ttatttttag gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg
     6781 agaaaaagag gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac
     6841 tttgtagaag tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt
     6901 tggacacttg cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg
     6961 aaaatttcag cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt
     7021 gaggatgcga tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa
     7081 tgaagatgat aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag
     7141 tagattatgc tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat
     7201 tattagtttt aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga
     7261 tccttcaact gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa
     7321 cgaataatac gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg
     7381 cacttttggt catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt
     7441 ttacagcaat tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa
     7501 caggcatgtc gattccaatc catgtattag taaatttagc agaacatcct aatatcattg
     7561 gtttgaaaga agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag
     7621 aagaattctt cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag
     7681 gaagcggtgt aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa
     7741 aagatacgca ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac
     7801 tgatccatgc cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat
     7861 tagacttacc ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga
     7921 aacaattagc tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat
     7981 tggcagtgga cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt
     8041 ccttctttta cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga
     8101 actgcttatt gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc
     8161 atatcaactt ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat
     8221 aaaagaacat gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat
     8281 ggtgatgaat caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact
     8341 gattgaaaaa catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct
     8401 tcaaacaatc caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag
     8461 agaagaaaac caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga
     8521 tgtattgttt gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg
     8581 aatctttgct gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta
     8641 ttacaaatta gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa
     8701 atcatccgct acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta
     8761 accgagccaa tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt
     8821 tttggtgact atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa
     8881 atcgaaatcg aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt
     8941 aatgctcgta tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc
     9001 gtgatcatga tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt
     9061 gatatgggtg ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca
     9121 ggagctgtct tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac
     9181 ggcgtactgg taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca
     9241 gtcgtggcag caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga
     9301 atacctgcac gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt
     9361 gctgcgttac gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt
     9421 gtgggggcaa ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt
     9481 ccagtaggac gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc
     9541 aagaaaaaga aacacactgt acaggaattg acagaaactt catttgaagg atatgattta
     9601 gcgctatttt ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag
     9661 ggactatttg tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc
     9721 gttccagagg tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt
     9781 tcaaccattc aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga
     9841 gtcaattatt cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt
     9901 catcgtacgc aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg
     9961 attccggaaa tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata
    10021 aatgagaccc gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt
    10081 gtgccaatcg aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg
    10141 acggaagaaa ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta
    10201 gcagcacata aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg
    10261 aggatcagaa aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac
    10321 atacgcaaag gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata
    10381 gcggaggtgg caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt
    10441 ctcatgaaga gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag
    10501 tgatcgttgt ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac
    10561 tttatttgat ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga
    10621 tttgtgatcg tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg
    10681 aactagcacg catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa
    10741 gtagaggtga atatctgact gctcaactga tggctgagta tcttggttac cagtttattg
    10801 acgcaaaaga cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac
    10861 aggaaatcac aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg
    10921 cgaaccctgc aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga
    10981 tactggcgaa gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca
    11041 tgatggctga tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag
    11101 aactgagaga gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag
    11161 ttcaagaggc tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga
    11221 caaaaatatc gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca
    11281 aggattttct ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct
    11341 ggaaagcaat gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga
    11401 tcgacaatat cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca
    11461 cgaaagaatt aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt
    11521 tgatctctgt cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat
    11581 cgatattgaa caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga
    11641 atctgatcat cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag
    11701 ggatggtgaa tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc
    11761 gggtttcatg aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc
    11821 gttatccatg aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa
    11881 acgattgctt ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct
    11941 tttgcttcta agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg
    12001 cttttagggt tgactgactt tataactaaa cacattgagg atcttattta taatgtagta
    12061 ctgatctatc agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga
    12121 ttgttggata aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct
    12181 aagggagaag ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt
    12241 atcattaaag gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag
    12301 gcagctgtac gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg
    12361 aatcatctaa tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga
    12421 gaagttattg tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag
    12481 aggggattag accatttagc agaacagttt cactctacag ctactatttc ttttcgctat
    12541 aatgacggct atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca
    12601 aacaaactac atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg
    12661 caacaatatc catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat
    12721 ccgacgttcg attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt
    12781 ctttttacag aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat
    12841 aatgatgaat ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat
    12901 acgacaattc gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga
    12961 accggatttt ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga
    13021 cttttctcac tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt
    13081 tcagcaacag cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg
    13141 tgcaacggaa gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct
    13201 tattccagcg cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt
    13261 gatcccgata gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc
    13321 atttgaacaa tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac
    13381 tgggacgaca ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc
    13441 ggtatttgtt ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc
    13501 catagctact tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc
    13561 gatgactggt tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat
    13621 taaagtccat caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga
    13681 agcgctaata aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag
    13741 agactatctg gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc
    13801 attttacttg ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt
    13861 agaattagcg gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg
    13921 agaaggttat atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa
    13981 acggatcaaa gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt
    14041 taattttgcg gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg
    14101 cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa
    14161 aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc
    14221 tcatggaggg catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat
    14281 cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt
    14341 agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca
    14401 atccaatgtt ca
//
LOCUS       DACJGB010000075        13949 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.077, whole genome shotgun sequence.
ACCESSION   DACJGB010000075 DACJGB010000000
VERSION     DACJGB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.077"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            131..505
                     /locus_tag="IYI34_002382"
     CDS             131..505
                     /locus_tag="IYI34_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAR0560221.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IYI34_002383"
     CDS             complement(619..1248)
                     /locus_tag="IYI34_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAR0560222.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IYI34_002384"
     tRNA            complement(1382..1454)
                     /locus_tag="IYI34_002384"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IYI34_002385"
     tRNA            1668..1741
                     /locus_tag="IYI34_002385"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IYI34_002386"
     CDS             1696..1845
                     /locus_tag="IYI34_002386"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560223.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IYI34_002387"
     CDS             2338..3666
                     /locus_tag="IYI34_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAR0560224.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IYI34_002388"
     CDS             3868..4701
                     /locus_tag="IYI34_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAR0560225.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IYI34_002389"
     CDS             4717..5454
                     /locus_tag="IYI34_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAR0560226.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="IYI34_002390"
     CDS             complement(5609..6577)
                     /locus_tag="IYI34_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAR0560227.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="IYI34_002391"
     CDS             6809..7642
                     /locus_tag="IYI34_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAR0560228.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="IYI34_002392"
     CDS             7676..8515
                     /locus_tag="IYI34_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0560229.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="IYI34_002393"
     CDS             8541..9101
                     /locus_tag="IYI34_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAR0560230.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="IYI34_002394"
     CDS             9300..11021
                     /locus_tag="IYI34_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAR0560231.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="IYI34_002395"
     CDS             11186..12328
                     /locus_tag="IYI34_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAR0560232.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="IYI34_002396"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="IYI34_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAR0560233.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4765 a         2305 c         2839 g         4040 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaa
//
LOCUS       DACJGB010000076        13819 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.078, whole genome shotgun sequence.
ACCESSION   DACJGB010000076 DACJGB010000000
VERSION     DACJGB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.078"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..222)
                     /locus_tag="IYI34_002397"
     CDS             complement(<1..222)
                     /locus_tag="IYI34_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560234.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDLYKVWDTEVVFLAIETSLLVN
                     LHYSNKNYFKIPAVKTRMKKGVYFLFDIVT"
     gene            complement(749..821)
                     /locus_tag="IYI34_002398"
     tRNA            complement(749..821)
                     /locus_tag="IYI34_002398"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(786..788),aa:Lys,seq:ctt)
     gene            1202..1771
                     /locus_tag="IYI34_002399"
     CDS             1202..1771
                     /locus_tag="IYI34_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAR0560235.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1758..2618
                     /locus_tag="IYI34_002400"
     CDS             1758..2618
                     /locus_tag="IYI34_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR0560236.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2808..3512
                     /locus_tag="IYI34_002401"
     CDS             2808..3512
                     /locus_tag="IYI34_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560237.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3517..5352
                     /gene="walK"
                     /locus_tag="IYI34_002402"
     CDS             3517..5352
                     /gene="walK"
                     /locus_tag="IYI34_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAR0560238.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5349..6665
                     /locus_tag="IYI34_002403"
     CDS             5349..6665
                     /locus_tag="IYI34_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560239.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6666..7535
                     /locus_tag="IYI34_002404"
     CDS             6666..7535
                     /locus_tag="IYI34_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560240.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7590..8399
                     /locus_tag="IYI34_002405"
     CDS             7590..8399
                     /locus_tag="IYI34_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR0560241.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8502..9131)
                     /locus_tag="IYI34_002406"
     CDS             complement(8502..9131)
                     /locus_tag="IYI34_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAR0560242.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9301..10593
                     /locus_tag="IYI34_002407"
     CDS             9301..10593
                     /locus_tag="IYI34_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0560243.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10669..11331)
                     /locus_tag="IYI34_002408"
     CDS             complement(10669..11331)
                     /locus_tag="IYI34_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAR0560244.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11556..11840
                     /locus_tag="IYI34_002409"
     CDS             11556..11840
                     /locus_tag="IYI34_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAR0560245.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11891..13516
                     /gene="groL"
                     /locus_tag="IYI34_002410"
     CDS             11891..13516
                     /gene="groL"
                     /locus_tag="IYI34_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAR0560246.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4628 a         2368 c         2938 g         3885 t
ORIGIN      
        1 agtcacgata tcaaacaaaa agtatacacc cttcttcatt ctcgttttca cagcaggaat
       61 tttaaagtag ttcttattag aataatggag attgactagc aggctagttt caattgctaa
      121 aaagactact tcagtatccc agactttata taaatctttg acaaattttt ctgatggatc
      181 gtatttaaac caaagcagat gttcctctag ctcgattgtc atgtttttca cctcaacgaa
      241 agtatacgaa ctaatgtttg ttttgtaaag gataaaaata gggtagcaac cggtacccaa
      301 actagtaccc atttatcaaa aacgacgaaa tttatgtaaa tagaaaaata agaataaaca
      361 ttgatgtatc aacatttata tatctttaga acaaatgaaa agggtggttc tcataagaaa
      421 aacatgttcg cacttctagg caaaccagga tttgaagacc ttgctaaaga attgaacgaa
      481 cgcctgtaaa ataaggattt gtcgctagat ttaaaaaatc agtgatagaa acatgtttgc
      541 tttaaaatga aactagtgat agttttagtg atagtgaact taaaaaaagc actcaatttg
      601 agtgcttttt tattgttatt aaaaattaaa aaactaaaat acatacccga atttcatacc
      661 cgaaatataa aaaatgtcat cacgtacatc tttttcgaaa ctaccgaagt agccaaagat
      721 gtactgatga cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc
      781 tgattaagag tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata
      841 atagtagcac taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt
      901 tacttcctta taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat
      961 accaacaaac aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc
     1021 tttcccgccg tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag
     1081 ccttcttgtt ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta
     1141 gattgaatgt ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat
     1201 gatggaaaaa atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct
     1261 gcctcactgg gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt
     1321 tgatcgttat ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat
     1381 ggtaaacaat tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa
     1441 agaacacgta gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga
     1501 aatcaaagaa gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct
     1561 attttgcgat gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc
     1621 tcatccgcaa aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac
     1681 tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt
     1741 aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg
     1801 atgtgccaga agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt
     1861 ataaagatca ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt
     1921 tgccagagga aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg
     1981 agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga
     2041 gcgggacata taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg
     2101 tactagatac gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat
     2161 ggctggaaga aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa
     2221 gaacaaaagt tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg
     2281 gtttagttgc ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg
     2341 aagaagggat ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa
     2401 cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc
     2461 atggggatgc gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac
     2521 aagcaaacca aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg
     2581 gattattggg tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag
     2641 gtgaaaatca gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg
     2701 cactttcatt ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata
     2761 cggtataatg agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt
     2821 tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag
     2881 gctatgatgt ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag
     2941 aacctgattt gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc
     3001 gtgaggtacg caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa
     3061 ttgataaagt cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca
     3121 accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg
     3181 aaccagaaga ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag
     3241 cttacatggt gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac
     3301 tcttttactt ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg
     3361 tttggggtta tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc
     3421 gtgaaaagat cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg
     3481 gttactattt gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt
     3541 cgatcagtca attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa
     3601 attattggtg cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa
     3661 gatatgaatc aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa
     3721 gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg
     3781 actagcgata tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc
     3841 gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc
     3901 acgactaaac gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa
     3961 ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc
     4021 gaacaaaaat attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt
     4081 gcggcattta tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc
     4141 ggtgaaatga gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa
     4201 gtgcatggac aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt
     4261 attgaagaag ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc
     4321 catatgacag acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat
     4381 atggctgttt ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca
     4441 cttttagata tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg
     4501 gtacttgacc gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt
     4561 tctatgatcc gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg
     4621 acagaacaag aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa
     4681 cttcgtacac cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg
     4741 aaagacgagg aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg
     4801 attcgaatga tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt
     4861 caattagaat atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg
     4921 atggtgggaa atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt
     4981 tgggtcgaag tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg
     5041 atcaagtatt caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac
     5101 gtggttttaa gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg
     5161 ttcgagcggt tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt
     5221 ggattagcta tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc
     5281 aaggaaggaa aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa
     5341 tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt
     5401 taagcatata cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg
     5461 tagatgagac aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct
     5521 ttcttccgct tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa
     5581 atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag
     5641 tagtttcagg agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt
     5701 tggcctataa tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag
     5761 atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc
     5821 aaggaaataa aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata
     5881 tttcgttgaa ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag
     5941 aaatgacaac agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac
     6001 tgaaaaagta tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt
     6061 tccagaaacc agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg
     6121 ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc
     6181 cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag
     6241 gcagttcttt agggaacctc agatattttg accgcagtga caatacgatc gattatcgga
     6301 tattcgtgga aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag
     6361 ttggcggtga aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc
     6421 aagtgccgat accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc
     6481 agttaaaagc taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat
     6541 ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat
     6601 ataaagataa ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga
     6661 attaattgga tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg
     6721 tgtttttatt cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta
     6781 atcagactga atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga
     6841 tttcgtctga gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt
     6901 cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag
     6961 acggtgatgt tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc
     7021 cagataaaat tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg
     7081 gtaatgatta tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc
     7141 aagcaagcca agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt
     7201 ctttagaaaa aaacgataat gattatagca tcacgaaata catgcagacg catttatctg
     7261 atatcgagca gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat
     7321 tgtacatcaa taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt
     7381 cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg
     7441 aaacaagcga taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga
     7501 caaacaatac tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt
     7561 tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca
     7621 gtatcctagc aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc
     7681 ttttggtcga tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc
     7741 ggacaccgga ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg
     7801 gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg
     7861 aagcgatgga accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg
     7921 ggaaagtcag aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag
     7981 cggctccgca attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca
     8041 ccggatattg cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg
     8101 aaagtaatca tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc
     8161 gtattttagg agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg
     8221 tgatcggtaa tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga
     8281 aagaacttgc tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac
     8341 atgattttga aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa
     8401 cagttcaatc aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc
     8461 ttctattttt atttttttag catattaaaa aaataaccgt attatcgact gactacactg
     8521 atgtgctgct tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct
     8581 gccatgctga tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa
     8641 taagtacctg tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa
     8701 tgtagatttt tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga
     8761 tagaagggtg catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag
     8821 aataacgaag aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca
     8881 atcaaatggg acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca
     8941 tcaacgacaa catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt
     9001 ataaacggaa catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa
     9061 tctctggaca atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt
     9121 gcaattttca taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat
     9181 tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca
     9241 ttgggtatag ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa
     9301 atgaaagagt ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga
     9361 cccttaactg ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg
     9421 ctgtttttct tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca
     9481 ggattttcta tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg
     9541 atcgtttttg ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc
     9601 ggcataacag ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat
     9661 actgtctgga caggtgctga agggatacta ggagcagtta caactgggct catcatcgga
     9721 tggggctatg cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct
     9781 gaaaatgttt ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg
     9841 cttatcggtt cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg
     9901 acatatgtcc agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta
     9961 ggtttttttg ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg
    10021 ttggttagtc ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga
    10081 caagagttga cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc
    10141 atgacggtta cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg
    10201 aaatcgaaga aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc
    10261 aatgaatcaa ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt
    10321 attttcgcac cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt
    10381 cctttattta cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta
    10441 gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt
    10501 tatttcccat ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa
    10561 aatgatggag cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag
    10621 attgggaaaa tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc
    10681 tttttggaca gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat
    10741 cgatgtccag atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa
    10801 gtagattagc agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc
    10861 tccgacccaa aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa
    10921 ctcttccatg atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat
    10981 catctggatg atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc
    11041 taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat
    11101 aacaatcttt ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg
    11161 cttaaaataa aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt
    11221 gatcaattca gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa
    11281 aattagacca tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct
    11341 ccttttaagg gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca
    11401 taatttcaat aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa
    11461 aaagagggag aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta
    11521 acagattaaa aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg
    11581 tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg
    11641 ctgctaaaga gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag
    11701 aaaacggtga aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat
    11761 acgccggtac agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata
    11821 tcatggcgat tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg
    11881 tgaatcaatc atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg
    11941 cggggtagat aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt
    12001 cgtactagaa aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa
    12061 agaaatcgaa ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc
    12121 atcaaaaaca aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc
    12181 aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg
    12241 cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt
    12301 tgattcaaaa gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg
    12361 acatttgatt gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga
    12421 atcaaaaggg atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta
    12481 cttatcacaa tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata
    12541 tatcttgatc acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca
    12601 aatcttgcaa caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt
    12661 gccaactctt gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc
    12721 tggtttcggc gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac
    12781 agtaattact gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa
    12841 tgcttctaaa gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa
    12901 agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga
    12961 ttttgaccgc gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt
    13021 taaagttggt gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc
    13081 attgaatgct acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct
    13141 agtgaacgtt atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat
    13201 caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta
    13261 tgaaggatca gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc
    13321 tgctactgga gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac
    13381 acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt
    13441 cgttgcagat aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat
    13501 gggcggtatg atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac
    13561 agataaaaag aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga
    13621 aattgctttt caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc
    13681 gctgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct
    13741 tttttgtgtg taattataat gaaaatagtt atttttaata gttataatat tttatctatt
    13801 aaaagtaaat atccattca
//
LOCUS       DACJGB010000077        13346 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.079, whole genome shotgun sequence.
ACCESSION   DACJGB010000077 DACJGB010000000
VERSION     DACJGB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.079"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(45..1364)
                     /locus_tag="IYI34_002411"
     CDS             complement(45..1364)
                     /locus_tag="IYI34_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0560247.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IYI34_002412"
     CDS             complement(1361..2014)
                     /locus_tag="IYI34_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560248.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IYI34_002413"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IYI34_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IYI34_002414"
     CDS             complement(2724..3440)
                     /locus_tag="IYI34_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAR0560249.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3490..3699)
                     /locus_tag="IYI34_002415"
     CDS             complement(3490..3699)
                     /locus_tag="IYI34_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560250.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3976..5249
                     /locus_tag="IYI34_002416"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IYI34_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IYI34_002417"
     CDS             complement(5356..6387)
                     /locus_tag="IYI34_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0560251.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IYI34_002418"
     CDS             complement(6394..7224)
                     /locus_tag="IYI34_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0560252.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IYI34_002419"
     CDS             complement(7221..8027)
                     /locus_tag="IYI34_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR0560253.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IYI34_002420"
     CDS             complement(8033..8857)
                     /locus_tag="IYI34_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAR0560254.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IYI34_002421"
     CDS             complement(8847..9947)
                     /locus_tag="IYI34_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAR0560255.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IYI34_002422"
     CDS             10125..10910
                     /locus_tag="IYI34_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAR0560256.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IYI34_002423"
     CDS             10943..11326
                     /locus_tag="IYI34_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAR0560257.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IYI34_002424"
     CDS             11402..11533
                     /locus_tag="IYI34_002424"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR0560258.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IYI34_002425"
     CDS             11502..11738
                     /locus_tag="IYI34_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR0560259.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IYI34_002426"
     CDS             complement(11816..12787)
                     /locus_tag="IYI34_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAR0560260.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4056 a         2912 c         2305 g         4073 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaaacaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DACJGB010000078        12742 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.080, whole genome shotgun sequence.
ACCESSION   DACJGB010000078 DACJGB010000000
VERSION     DACJGB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.080"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            101..544
                     /locus_tag="IYI34_002427"
     CDS             101..544
                     /locus_tag="IYI34_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0560261.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IYI34_002428"
     CDS             complement(550..1734)
                     /locus_tag="IYI34_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAR0560262.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IYI34_002429"
     CDS             2002..2700
                     /locus_tag="IYI34_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAR0560263.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IYI34_002430"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IYI34_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAR0560264.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IYI34_002431"
     CDS             complement(3510..4043)
                     /locus_tag="IYI34_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560265.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IYI34_002432"
     CDS             complement(4340..6721)
                     /locus_tag="IYI34_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAR0560266.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IYI34_002433"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IYI34_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAR0560267.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IYI34_002434"
     CDS             7422..7961
                     /locus_tag="IYI34_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAR0560268.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IYI34_002435"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IYI34_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAR0560269.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IYI34_002436"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IYI34_002436"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IYI34_002437"
     CDS             9068..10234
                     /locus_tag="IYI34_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAR0560270.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IYI34_002438"
     CDS             10280..11776
                     /locus_tag="IYI34_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAR0560271.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IYI34_002439"
     CDS             11857..12540
                     /locus_tag="IYI34_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR0560272.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3609 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta tt
//
LOCUS       DACJGB010000079        12734 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.081, whole genome shotgun sequence.
ACCESSION   DACJGB010000079 DACJGB010000000
VERSION     DACJGB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.081"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IYI34_002440"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="IYI34_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAR0560273.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="IYI34_002441"
     CDS             complement(323..892)
                     /locus_tag="IYI34_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAR0560274.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="IYI34_002442"
     CDS             1078..1980
                     /locus_tag="IYI34_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR0560275.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IYI34_002443"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="IYI34_002443"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAR0560276.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IYI34_002444"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="IYI34_002444"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAR0560277.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IYI34_002445"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="IYI34_002445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAR0560278.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="IYI34_002446"
     CDS             8548..8733
                     /locus_tag="IYI34_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAR0560279.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="IYI34_002447"
     CDS             complement(8795..9991)
                     /locus_tag="IYI34_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAR0560280.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="IYI34_002448"
     CDS             complement(10161..11597)
                     /locus_tag="IYI34_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAR0560281.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11890..12699
                     /locus_tag="IYI34_002449"
     CDS             11890..12699
                     /locus_tag="IYI34_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0560282.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3801 a         2673 c         2250 g         4010 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttccact ccctgaattt ttttctgatg gaaaactata
    11641 ataataaaaa ataagtaaaa tagggcaaat ccatcgaaca atttcaaatt atatagacgt
    11701 ttcattcgct tttcaacgtt tttttaacaa aaaaagcgaa aataatactt aacgttcgtg
    11761 tttagcttat gtttagctat ttatatagcc ataactgtag tttttgttag gaatagctga
    11821 ttgctcttgt taattaccta aaatatgcta gactaaaaca aatgctagtt gaggaaggaa
    11881 ggacccttat tgaataaaaa attaattgcc atcgatctgg acggaactac cctcaatcaa
    11941 gattcgatga ttacatctaa aacaaaagag actctaaaaa aagcaatcaa tgccggtcat
    12001 catgtcagta ttgctacagg acgcccattc cgaatgagcc atcagtttta ccaacaatta
    12061 gaattaacaa caccgatggt taattttaac ggcgcattag tacacatccc taatcagcac
    12121 tgggatggtg aaagagaaac gttgatcaat cgtgaaatcg cttttgaaat cttatcgcaa
    12181 aaaaagcagc taaatttaga ttttatcgct gctgaaaatc gtgatacttt ttttattgac
    12241 agttttgatt tctttgatga aaaaattttt gcttcttctc gtccaggaga gaagaatttg
    12301 ctttctccta aaaatttgac gacaaatcct acatctttgc ttgtccgaac agacaaacgt
    12361 tttgcaggag ctgtatctgc tgaattaaca cgtcaatttg gcagttatgt cgatgttcgg
    12421 acatggggtg gacctactgc catcttagag attgtttcaa aaggaatcca aaaagcaaaa
    12481 ggcgtgcaag agattgctaa ctacctgtct atcgatcaaa aagacgtcat tgcctttggt
    12541 gacgagcata atgatttgga attgttggat tatgcaggtt ggggagtagc tatggctaac
    12601 ggtactgacc aactcaaagg cattgcaaat gatgtcactc ctttgagcaa tcaggaagac
    12661 ggtttagctg tttatctgga aaaactactt aaactataat agagaattag attccacaaa
    12721 aaagaggttg tgac
//
LOCUS       DACJGB010000080        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.082, whole genome shotgun sequence.
ACCESSION   DACJGB010000080 DACJGB010000000
VERSION     DACJGB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.082"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            6..305
                     /locus_tag="IYI34_002450"
     CDS             6..305
                     /locus_tag="IYI34_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0560283.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IYI34_002451"
     CDS             378..1739
                     /locus_tag="IYI34_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAR0560284.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IYI34_002452"
     CDS             1761..2021
                     /locus_tag="IYI34_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560285.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IYI34_002453"
     CDS             2035..2787
                     /locus_tag="IYI34_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAR0560286.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IYI34_002454"
     CDS             complement(2881..3486)
                     /locus_tag="IYI34_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR0560287.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IYI34_002455"
     CDS             complement(3539..4513)
                     /locus_tag="IYI34_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAR0560288.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IYI34_002456"
     CDS             complement(4891..5670)
                     /locus_tag="IYI34_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0560289.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYI34_002457"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IYI34_002457"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAR0560290.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IYI34_002458"
     CDS             7161..8558
                     /locus_tag="IYI34_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR0560291.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IYI34_002459"
     CDS             8639..9079
                     /locus_tag="IYI34_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR0560292.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IYI34_002460"
     CDS             9227..9553
                     /locus_tag="IYI34_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR0560293.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IYI34_002461"
     CDS             9554..10303
                     /locus_tag="IYI34_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560294.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IYI34_002462"
     CDS             complement(10383..10838)
                     /locus_tag="IYI34_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAR0560295.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IYI34_002463"
     CDS             complement(11265..11540)
                     /locus_tag="IYI34_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560296.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IYI34_002464"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IYI34_002464"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAR0560297.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4054 a         2175 c         2588 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACJGB010000081        12432 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.083, whole genome shotgun sequence.
ACCESSION   DACJGB010000081 DACJGB010000000
VERSION     DACJGB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.083"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..190)
                     /locus_tag="IYI34_002465"
     CDS             complement(<1..190)
                     /locus_tag="IYI34_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560298.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="IYI34_002466"
     CDS             complement(200..1015)
                     /locus_tag="IYI34_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAR0560299.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="IYI34_002467"
     CDS             complement(1277..1720)
                     /locus_tag="IYI34_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAR0560300.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="IYI34_002468"
     CDS             complement(1854..2192)
                     /locus_tag="IYI34_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAR0560301.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="IYI34_002469"
     CDS             complement(2180..2557)
                     /locus_tag="IYI34_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAR0560302.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="IYI34_002470"
     CDS             2781..3974
                     /locus_tag="IYI34_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0560303.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="IYI34_002471"
     CDS             complement(4140..4562)
                     /locus_tag="IYI34_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0560304.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="IYI34_002472"
     CDS             4957..5151
                     /locus_tag="IYI34_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560305.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="IYI34_002473"
     CDS             5148..5594
                     /locus_tag="IYI34_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560306.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="IYI34_002474"
     CDS             complement(5804..6976)
                     /locus_tag="IYI34_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAR0560307.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="IYI34_002475"
     CDS             complement(7182..7997)
                     /locus_tag="IYI34_002475"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAR0560308.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="IYI34_002476"
     CDS             complement(8147..8593)
                     /locus_tag="IYI34_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAR0560309.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="IYI34_002477"
     CDS             complement(8590..9594)
                     /locus_tag="IYI34_002477"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAR0560310.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="IYI34_002478"
     CDS             complement(9634..9963)
                     /locus_tag="IYI34_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAR0560311.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="IYI34_002479"
     CDS             complement(10089..12416)
                     /locus_tag="IYI34_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAR0560312.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4217 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctcaata aa
//
LOCUS       DACJGB010000082        12163 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.084, whole genome shotgun sequence.
ACCESSION   DACJGB010000082 DACJGB010000000
VERSION     DACJGB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.084"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            158..730
                     /locus_tag="IYI34_002480"
     CDS             158..730
                     /locus_tag="IYI34_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAR0560313.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            962..1579
                     /locus_tag="IYI34_002481"
     CDS             962..1579
                     /locus_tag="IYI34_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006845390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAR0560314.1"
                     /translation="MNYIAEKAHTKQYQKGEFLFRSQEKEDTLYIINRGKIRIYRLAD
                     SGKEQLVRILNPGDFTGEWTLFNPDAVHEEYAEATRDTVVCMIQQKDVQDFLEQYPAI
                     SLKLLAEMSKRLESSERQTTQVAVESVITRLVLFLAENVEPEMGNSPTITLPMAKKDI
                     ASYLGTTPETISRKFGELEDEGLIQQLSGKRIKIQDLDDLLLYSE"
     gene            1807..2145
                     /locus_tag="IYI34_002482"
     CDS             1807..2145
                     /locus_tag="IYI34_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560315.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            2130..2984
                     /locus_tag="IYI34_002483"
     CDS             2130..2984
                     /locus_tag="IYI34_002483"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAR0560316.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            3131..3364
                     /locus_tag="IYI34_002484"
     CDS             3131..3364
                     /locus_tag="IYI34_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HAR0560317.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            3562..5466
                     /locus_tag="IYI34_002485"
     CDS             3562..5466
                     /locus_tag="IYI34_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAR0560318.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            5776..6138
                     /locus_tag="IYI34_002486"
     CDS             5776..6138
                     /locus_tag="IYI34_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560319.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            6155..6766
                     /locus_tag="IYI34_002487"
     CDS             6155..6766
                     /locus_tag="IYI34_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HAR0560320.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(6868..7776)
                     /locus_tag="IYI34_002488"
     CDS             complement(6868..7776)
                     /locus_tag="IYI34_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAR0560321.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(7818..8132)
                     /locus_tag="IYI34_002489"
     CDS             complement(7818..8132)
                     /locus_tag="IYI34_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAR0560322.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            8431..10362
                     /locus_tag="IYI34_002490"
     CDS             8431..10362
                     /locus_tag="IYI34_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAR0560323.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            10655..11206
                     /locus_tag="IYI34_002491"
     CDS             10655..11206
                     /locus_tag="IYI34_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR0560324.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            11519..12061
                     /locus_tag="IYI34_002492"
     CDS             11519..12061
                     /locus_tag="IYI34_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560325.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         3952 a         2034 c         2555 g         3622 t
ORIGIN      
        1 aatttaatag agaagcctgg cgtctattaa gaaataattt tcttgatgag cgtcaatttt
       61 tttaacggaa atctatccta tactaaattc agtaaagaaa aaggaggaat caaagaaagc
      121 tattttaaac aaaccaaaat tataggagga ataaatcatg actgaaaata aagctactca
      181 agaaagaaca ccccaagaaa gattgcaaga agaacaagaa cacaaggaac atgttcatca
      241 tacgaaaatt aatgcagcgg ccattgcgga tcatctttta ggcaacatgc atacattaca
      301 tgtgaaactg caccaatatc actggtatgt aaaaggagcc aatttcttta cgttgcatga
      361 aaaatttgaa gagttgtata acgaaaatga aaaatggttc gacaaacttg cggagcgtct
      421 aattacttct gggcataaac ctgcttcgac aacggttgaa tttgaaaaat attcgatgct
      481 ttcggaagat gcagttaata aatacgctaa agcagaagac atggttgaaa atattatcga
      541 ggacttcaga agcactcgtg acctaactat tcgcgcgatt cgtttagctc aagacgaggg
      601 tgacgatgct ttagaagata cattgattgc ttttaaaaat gacttagaca agaacatttg
      661 gatgctgcaa gcgttcattg gtaaagaagc attagaagat gatgacgctc ttgatgagga
      721 tgaagattaa ggtaaaagta gaagctgatt tagggattgg aattctttcc agtccttttt
      781 ccattttctt gatgtgcatc aatttttttg tagtaagaga cttatataat aaaagcagcg
      841 aatataaaca aaataaataa agggggagaa tgaattgact aaacataccc atcatgaaat
      901 tggcgaccac gcagcctgta tccgcttggt tccaatattc aatcacttag aggatagcgc
      961 gatgaattac attgctgaaa aagcccacac gaaacagtat caaaaaggag agtttctgtt
     1021 tcgttcacaa gaaaaagagg atactttata tattatcaat cgtgggaaaa tccgtattta
     1081 tcgattggca gactctggca aagagcagct agtgcggatc ttgaaccctg gtgatttcac
     1141 cggagaatgg accttgttca acccggatgc agtacacgag gaatatgccg aagcaacccg
     1201 agataccgtt gtctgtatga ttcagcaaaa agatgtccaa gattttctgg aacaataccc
     1261 agctatttct ttaaaactat tggcggaaat gtctaaacgg ttagaaagtt cagaacgtca
     1321 aacgacacaa gtagccgtgg agagtgtcat tacccgttta gttttgtttt tggcagaaaa
     1381 tgtggaacct gaaatgggca actctcccac catcaccctg ccgatggcaa aaaaggacat
     1441 tgcttcctat ttaggaacga cacctgagac catcagtcgc aaatttggag aattggagga
     1501 cgaaggattg attcaacagc tgtctgggaa aaggatcaag attcaggatt tagatgattt
     1561 attgctttac agcgaataat cggacacact tttttctaca cgttggtgtt ctgtcgctct
     1621 tcattaacaa cgggacagaa ctcagtgctt ttgcttccat ctgtccgttt ttggcttttt
     1681 taagacagaa cgaccttcat tcattggaaa aataaaagca caaacatgat aaccctcaag
     1741 tttttttgtt ttatttttct ttaagatagg gttgtcgaac aaaacgtaaa aataagtagg
     1801 gtgaatgtgc attgtttttg ggtgatagtt gccatcacgt tgtcagcagc gggggcattc
     1861 tttgtcgtcc ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt
     1921 gaggcagata ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc
     1981 ggacacggaa aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact
     2041 gtttggtttc catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg
     2101 cgaaaaattg aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc
     2161 atgtggtgga gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca
     2221 tattagcggc tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata
     2281 ccattatcga tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt
     2341 atgtgttatt tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga
     2401 atggcggcat tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg
     2461 caaagaaaaa aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt
     2521 tagcccagtc cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg
     2581 ttactcgtca attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata
     2641 tgaatggtac ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct
     2701 ttgtcctcat cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt
     2761 tgagttacgt tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca
     2821 gtttatggat tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg
     2881 gcgcgattgt agtatggttt atacgcagac aagattatcc accaatttct tattattctg
     2941 atggcaataa ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag
     3001 aagtgcaaaa cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg
     3061 taaagaggaa aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga
     3121 gataggaatt atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat
     3181 gcaaaaaatt gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt
     3241 attattcaac tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga
     3301 gattcaaaaa gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt
     3361 ttaacagatt aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt
     3421 ttttgttgca atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga
     3481 tgtcagtcaa ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa
     3541 ttaaatttgg agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc
     3601 gtcatcagtg gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta
     3661 ggactttcag agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt
     3721 caagcgtttc aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca
     3781 gcgattggtg cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc
     3841 ttattcggac actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa
     3901 ttgactgaaa tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa
     3961 gtagaagtgg atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt
     4021 ccagtagatg gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt
     4081 gaagctattc cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa
     4141 aacggaacga ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt
     4201 attgaactcg tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc
     4261 ttttctaaat actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt
     4321 caaaatattg agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc
     4381 ggagtgcctg tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta
     4441 aaagggagtg aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca
     4501 ggtactttaa ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct
     4561 gctgaaacgc ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa
     4621 gcggtcttaa atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg
     4681 aaaggcggcg gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc
     4741 ttgatggaaa aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt
     4801 gaacaacaag ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg
     4861 ggcattcgcg atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg
     4921 ggcgtgaaaa atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc
     4981 ggcgaacttg gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat
     5041 atcggaaaac ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat
     5101 agtccgtcct tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg
     5161 attgaaacat ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta
     5221 ggattagtaa aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta
     5281 gtgttggtct tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg
     5341 ttggttcatg aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat
     5401 aagttgaaag gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata
     5461 tcttaagaat aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg
     5521 aaactcagat ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga
     5581 gtatagagta tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat
     5641 aaaatttaaa ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat
     5701 taaggtagat tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa
     5761 tgtgaggtga gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta
     5821 aatgtaataa aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa
     5881 tgtttcagcg attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg
     5941 tgtgtctgtg atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta
     6001 cgttttttaa aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat
     6061 tctttagcga atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat
     6121 ctatctatga aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat
     6181 cgctgtttat atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca
     6241 aagccacaca aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat
     6301 tttggtagcc gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat
     6361 cattggtctt ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga
     6421 agaggaggaa gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt
     6481 attctggaca gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat
     6541 tccatacttt acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat
     6601 ctctgttgcg gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga
     6661 aacgttagaa aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt
     6721 tattatgata gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg
     6781 aaagataaaa agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa
     6841 tgaagtattt tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc
     6901 ttctttgctt gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac
     6961 ttctgcaatt tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac
     7021 cgtgatgata agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc
     7081 ttttacagca ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg
     7141 cgaaccattc aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat
     7201 ataaagtgca atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat
     7261 tagaatactc acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt
     7321 caacatgctc tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa
     7381 tgcaatagct acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat
     7441 caaacgtggc acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt
     7501 tatcagcgcg cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt
     7561 tttctctgaa taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc
     7621 atgaactgcg tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct
     7681 tatagagaaa ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg
     7741 attcgagtga ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg
     7801 ctacatacta attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga
     7861 atactaaaaa catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat
     7921 ttcactaagc gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg
     7981 tccagagaaa tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc
     8041 gacaatcgtg taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct
     8101 ttatcgattg gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt
     8161 ccgttcatat ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag
     8221 aagtggcgtt ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac
     8281 atatgattaa ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga
     8341 aaggagtcag aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg
     8401 aattataaat tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa
     8461 gaaaagcata atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc
     8521 gttttgtact ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat
     8581 cagttggtac aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt
     8641 cttcttgaag gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct
     8701 aattctcata ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg
     8761 gaaggaacat tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa
     8821 ggaagaagca aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta
     8881 atcctgcctg atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa
     8941 cttcaagtgt tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca
     9001 tccattgatg agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat
     9061 ggcgtcttcg ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa
     9121 aacaaagata cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa
     9181 acaaaagctg cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt
     9241 gccattcctc tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt
     9301 gtctatagag gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact
     9361 gtctcggcaa cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt
     9421 agttcttacc tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc
     9481 ttgaccaaag gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa
     9541 aacatgattg atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct
     9601 attctaagaa agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt
     9661 aaaggattga cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt
     9721 gatgatgttt cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca
     9781 gtcgtatacg tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg
     9841 agtgaatatg ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta
     9901 attactggtg actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa
     9961 gtgattgcga atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa
    10021 tacggcgtga ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca
    10081 gatgtgggaa tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt
    10141 ttaatgcaaa acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac
    10201 cgtgttattt ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt
    10261 agtttcttag gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg
    10321 gttgttattt tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt
    10381 caaattaatc gagtaataga gataattaag gattacacta cattttttat ctctattact
    10441 tgtttatatt tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg
    10501 cgggttgaat ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta
    10561 tagggttctt tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc
    10621 atgtattcga acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg
    10681 gtcgcaacag gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg
    10741 tgacgttttc ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa
    10801 actcaagaaa aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg
    10861 gaaaacacgc attttatact taagcagctt acaggacttg attcacggct ataccgcacc
    10921 cttagatccg gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac
    10981 ggtctaccag atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca
    11041 caccctacga aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt
    11101 aatggaaatc tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga
    11161 agatgaaatc agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat
    11221 agataagcac agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt
    11281 ggtatattat atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca
    11341 taagggtccg gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg
    11401 gtccggcggg tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc
    11461 tttttttatt gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat
    11521 gtctataaaa gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca
    11581 tctcttacaa gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt
    11641 agcgattatt ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg
    11701 cacacacttg ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat
    11761 caaaacggat gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt
    11821 ggggaagcat atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc
    11881 tattcgaaca aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat
    11941 ccctatctct ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg
    12001 catttttagc gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta
    12061 atcattaata gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa
    12121 taaagcattt taagacgatt tcaaatcatt catgtgggtt att
//
LOCUS       DACJGB010000083        11983 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.085, whole genome shotgun sequence.
ACCESSION   DACJGB010000083 DACJGB010000000
VERSION     DACJGB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11983
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.085"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            960..1928
                     /locus_tag="IYI34_002493"
     CDS             960..1928
                     /locus_tag="IYI34_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAR0560326.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1945..2469
                     /locus_tag="IYI34_002494"
     CDS             1945..2469
                     /locus_tag="IYI34_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAR0560327.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2845..3126
                     /locus_tag="IYI34_002495"
     CDS             2845..3126
                     /locus_tag="IYI34_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560328.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3128..3544
                     /locus_tag="IYI34_002496"
     CDS             3128..3544
                     /locus_tag="IYI34_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560329.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4174..5328
                     /locus_tag="IYI34_002497"
     CDS             4174..5328
                     /locus_tag="IYI34_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAR0560330.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5322..5744
                     /locus_tag="IYI34_002498"
     CDS             5322..5744
                     /locus_tag="IYI34_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560331.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            6055..6435
                     /locus_tag="IYI34_002499"
     CDS             6055..6435
                     /locus_tag="IYI34_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAR0560332.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            6417..7331
                     /locus_tag="IYI34_002500"
     CDS             6417..7331
                     /locus_tag="IYI34_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAR0560333.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            7291..7569
                     /locus_tag="IYI34_002501"
     CDS             7291..7569
                     /locus_tag="IYI34_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560334.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            7576..8265
                     /locus_tag="IYI34_002502"
     CDS             7576..8265
                     /locus_tag="IYI34_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560335.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(8386..8472)
                     /locus_tag="IYI34_002503"
     CDS             complement(8386..8472)
                     /locus_tag="IYI34_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAR0560336.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            9109..10053
                     /locus_tag="IYI34_002504"
     CDS             9109..10053
                     /locus_tag="IYI34_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAR0560337.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            10074..10607
                     /locus_tag="IYI34_002505"
     CDS             10074..10607
                     /locus_tag="IYI34_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAR0560338.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            11247..11471
                     /locus_tag="IYI34_002506"
     CDS             11247..11471
                     /locus_tag="IYI34_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAR0560339.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            11491..11778
                     /locus_tag="IYI34_002507"
     CDS             11491..11778
                     /locus_tag="IYI34_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAR0560340.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT         4570 a         2037 c         2070 g         3306 t
ORIGIN      
        1 cactttagtg agttttgtag aaagtacctt taaaggtact ttcacattac gaacgatacg
       61 tccgtaggac gtactgaaag ggtgggattt atgagcgaac taatgcaaaa tttggctagc
      121 aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat gaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgcctaga
      241 cttttaaaaa agtcggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgtctaagta tataaatata taagagtagg tttaagagtg cttaaatcga
      361 attttagagg gcattcagac aactttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgctttaag aacaagataa gggatttata ctaagactgt cttaaaacgt ctcaaacgaa
      481 ggctcatagc ttctcaaaga gttccttatg aacattttta gcaaaggagc cggaagcgac
      541 tgcgcagtat aatgtgagag cagagcgatc acaccttttt aagcgactgt agggagtaag
      601 attcccagtt gagaaaaatg cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc
      661 gggaaatttt gtccccctct tttataatct ctttttaaac ctctttttaa acctctttac
      721 cgtcggctat atccttactc tcccaaggga atgacgcttt tatctacatc gttttgtctg
      781 tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc tacatcgttt
      841 tgtctgtttg tacattgaat tagtacatcg acgatgttaa tataagatgt agataaacag
      901 gcattttgta tttcataacg tgtatctctt gtgcgagtaa gaaaggagct attttaaata
      961 tgtctaatga aatcgtaaag tatcatcatg aattaaatac aattccgttg cgaaaattta
     1021 caccagtcga aatgaatttg ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata
     1081 aaaaagtaca atttacattc gagcaattaa aagatttaag caattataaa gcaactgcaa
     1141 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     1201 gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt tttatttaat caatttaaaa
     1261 tcgacggaaa atctgatatt ccttttgctg aaatacaagt tcatgaaaaa gccttaccct
     1321 tacttaataa tcttgaagaa tgggtgcgct attctctcca acaattcaat gaattagaaa
     1381 gcagttattc aaaaacaatg tttcgattac taaaacaata cagaaccaaa ggtttcgctt
     1441 atttttcaaa agaagacttt catgaattac ttgatattcc taaaagttac aaccaaggta
     1501 cgattgatca acgtgtgttc aatccaatac gacaagaact aaccgcaatc tttaaaggtt
     1561 taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     1621 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgcccca acaaaagaag
     1681 aacaaaacaa agcattagaa ctagctaacc aacaagttca atctgaaaaa gcacctactt
     1741 cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga acttttacaa cagttagaaa
     1801 agttagaaag agaacaaaat acccaaaata aagaaaataa tgcacaaaaa tcagagataa
     1861 attccgatat tccttcagaa ctaaaaaaag acttattaga caatcttcaa aacttattca
     1921 aagattgagg ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa
     1981 ttaggagtta gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg
     2041 ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca
     2101 atgtttttta acgaaaacga aaactcaaat caacaactag ttggcgaaaa aacgaaaacc
     2161 gaaaaccaca aaaacgccaa ccaagttccc gaattagaag tcctttctct cctagaacaa
     2221 cagttaaatc aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa
     2281 aaattactcg accaccaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
     2341 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
     2401 tctgaagaaa cagaatcgat agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc
     2461 tggcaataaa accagcaatt taaattaata aaaattgctg gttttattct tctttttgct
     2521 tttgcgagca gagcaacatg gacccatgtt gcgatatttt ggtggcgccc caatccccgt
     2581 tttttcgttg aatcttactc ggactgcact cgttttacag ctgatttttc tgaattttga
     2641 aagagcataa ggaaacgtgg tatcactttc ttacgaaagg tgctagatag gctgtgccta
     2701 tcacgtttct cagaaaaaaa tttgctatca cttttttgcc attgtggcta ggctatgtat
     2761 agtgttttgg cgaaaaatag ctatacaaat actatatttg tatggtatac ataaattata
     2821 cgctgatacg aaaggaaaat ttccatggac attttagttc gaaatatcaa tcccaaaatt
     2881 attgcaagaa tagataaacg ttgtcaggaa ctcacaacca aaacgggcaa aaaatggagt
     2941 cgaaataaat atctaaaagt tctaatcgaa agtgattttg accgtcaact tatgaattac
     3001 aaaaaagatc aattcgattt attactcgaa aaatttattg ccattcaaac agaaaatacg
     3061 aacatactga atgactatgt tcacacaaac aaccaactca ttgaaacact tatcggagga
     3121 atataacatg agaaaaactt ctgttcaaaa acacgtaacc ctttatccca gtcacgttga
     3181 cgttttagat tccatcatcg aaaaaaatcc agacgtcaac aattattcag aagccattcg
     3241 ttttttatgt atgagttatg aacctgatac caatacaacc acaaccagta accaagcaaa
     3301 actcaatgcc atgggaaaag aaatttctat catgacagaa atcatggcag aatttgctga
     3361 tttatatcta aaagatactg gtgttcttat cggtacaaat tctaaagtgt atcaagaagt
     3421 gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga aatcaaccca aaaatcgtca
     3481 aaaagaaacc aatcaaacct ttacttctgg aaattttgac ccaacaaact ttctaaaaag
     3541 ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt ctttttaggg atcagtcttc
     3601 cagcaagatt tgcaagttgg taccaacttg cgaaaaaaaa gaaaaatccg cttatgcaac
     3661 ttcttctttt tgtattttgg ggcgctaccc caatccccaa gtatcggtta tttaatagcg
     3721 cggaaatcca aagaatagga cgtaccctga aacaaaaaaa gactgctctc acgagcagcc
     3781 caaaatgaga ttatcctatt catcaacata aaaatattac aaagtgaaga actagagaaa
     3841 gacaaaaaaa gcatatcaaa tcctctcaaa aataacaagt ctttaaaaaa tgacagataa
     3901 aaatgcacaa agaactaaaa aagagaaatc gcgtaaaatc aagcattaga agctagtaac
     3961 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     4021 tggtctagtc gacattatgt taactagctc gataaataac gattatgttc ccaaaacaat
     4081 attttatgaa ctataacaaa aatttgttat cgcatattgt atattttaat tatataatta
     4141 taggtgtata ccacttaaat ttaacacaaa gacgtgatta ttttgaaacc caaaaagaaa
     4201 atttatctag gtctcctctt ttccttgatg attggaattt ttagtatagg aattacaaat
     4261 acctatgctt cagaaaattc taatttcaat atcaactccc aaccaattga agaacaagca
     4321 attaattttg cgaaaactga ttttgaacac tccataactt caattctttt aatggaaaat
     4381 aacaccgaaa ctgatattaa ctatactctt ggttcgccat ttaaaatgct cgatggagaa
     4441 aatacggcaa atagttctat tttctttcca gtattttcag acgctgataa tacgattaaa
     4501 tatatttata ccgtcaaaaa aaatacaaat ggagaatatt caggaagtat ctcacaattt
     4561 ttagcaaaag aacttcaaaa tctagtagat agtaatataa cagatacaac cttatttttt
     4621 caagataata gcatttatta ttccgataat ggacaaatta aactactttg gaaatcacct
     4681 ttaagtacag aaaaaaatgg tgtttcttcc tttacagaaa aaaatgatct tatatccgta
     4741 aatataaaaa ataaaattaa aaatttctct aaaaaacaaa attttcctgc ggttaaacgt
     4801 gtaaaaagtg tctcctccgg agataacttt attatgatta attggcgtat agcagaaacc
     4861 caaacaagtt ttccgtggtg tgctgcttac gtagaagctg ctatcttatg taacaaaaca
     4921 gatgtacgtg ctacttcagc tagagccatt atgcattata cctatcctaa tttaaacaac
     4981 agtcaaattg aatcaaaaag tcttaatcaa gatcaaataa taaaatatgc aaattactgt
     5041 ggtgtttatc ctaaaaaagt agcacgaata ctaaaccttt cagaggtcca aacacaacta
     5101 agaaaaggaa atgccattta tatgggaact gctggttatg gaaattatag taacagccgt
     5161 catgcacttg cattatttgg gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg
     5221 aatccttggt ataactaccc aactgtaata agtgcaaatg caggaccagt tacaattcct
     5281 gtacctggag gtggttttac ttgggacaat accattacaa attggtaaat aaacccattg
     5341 ctttagttat gggtctagtg ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa
     5401 tacaaaagac tgatctgatt ccttttctag gaacttggca aactaaagat cattcagaac
     5461 gtctagtaat cactaattta aatacttcaa aaaatataga acaatatacc atcttagtaa
     5521 aagagaacaa tcataaaatt caaatcattg ataaaaattt aaatataaat gaacaagttt
     5581 tcatttacaa agcaacaaat aatttactaa cactgcgctc taataaaaaa caattagatt
     5641 acacttttag tttggattct ccaaaaaagc tcgtatttat tttgacaacg aatgatggga
     5701 cagaagggac agctaaacca attgaatttt ttaaagctga ttgatgaata gttgtttgat
     5761 attacaaaac aataatttca ccgaaggtga aacaagcgaa actttacaaa aagtggctag
     5821 catttgctag ccttttttgt attgtggagc gtgggttttg agggggattc aagggggagc
     5881 ttgcgcccct ttgacaagcg gttatttgca ccaaatacgg aggtatttgg ctagcaaata
     5941 tagagcttgc caagcccttt agtaagcttt agtgagcttt acttattgta cctttttagg
     6001 tacaatggta ttgcgaacga tacgtccgta ggacgtactg aaagggtggg acttatgagc
     6061 gaacaaaatc aatatttggc tagcaatcat tctaaaaaat atacctatcg atccgagcca
     6121 aaacaaatca gttttcgagt gagcgaatcc gaatttgcaa agctgaagca gtcagctgaa
     6181 gctttgcaaa tgagtgtgcc ggctttcgtg aaagccaagg cacaaaacgc gcgtctggtg
     6241 acaccgaaag tggcgccaga cattgctcag gcaatcgcgc gcgatttagc caaagctggc
     6301 gggaatatca atcaaattgc caagtggtgc aacacacacc aacacgacgt tgatcctggc
     6361 gacgcccaac gcttatccga aaacctaaaa atcatgcaaa aggagctaca aaagatatgg
     6421 caacagttaa agtaagccga accacttctt gtagccgagc gatcaattac gccgaaccac
     6481 gagcaaccgt caaaacaggg ataaactgtg acattgatta tgccaaaagc gaaatgaaac
     6541 aaattcgcat gctgtatgga aaagacgatc gtgtacaagc acacttattg atccagtctt
     6601 ttcgaccagg agaaattacc gctgaaaaag ccaatcaact aggggcagag tatgcgaaaa
     6661 aaatagcccc tgaccaccaa atcgcgattt acacacatac agacaaagac catattcata
     6721 accacattgt cattaactcc gtcaatctgg aaactgggaa caaatttcaa gcccatggtc
     6781 aagcattttt aaacaaatgt tatgacctaa atgacgagat ttgtcttgaa catgacttaa
     6841 caatcactga acgagggaag aaacccgaaa aacgaaccat gtctgaaatc aaattaaaag
     6901 aaaaaaatga acctgtttgg aaagacgaaa ttcggtttgc gattgatcaa acaatgaaaa
     6961 acaaaaaaac tcgaacgtat aaccaatttt gtgattctct taaaacattc ggcattcatt
     7021 gcttgaatcg tgggaaaaat tttacttacg aattgatcca caaaaagaaa aaagtacgat
     7081 ccaacaaact aggaaaggat tacgaaaagg agacgatttt acatgagctg gacagacgag
     7141 aacgagccta caatcaacaa cgaaccaacg agctacgaag agctcgagct agcattcgcg
     7201 aaactgcaaa agaagtacga agagaacaac gaactcttac gactgaacaa ccggaaatta
     7261 gaccgactcg aagaaaccat gaccaacacg ttgaaagagg agcagactac caacaagaat
     7321 tatcgttcta aaacgttaca agacatagaa aatgaaataa agaaacaacg tgcttttctt
     7381 gcaccttata ccaaagaaag aatacaaact acaattgatt cacaagtcaa aaaattccac
     7441 catgcgctat taaaccagcg aaataaagaa cagaaaaaag taattatgca gcgatcaatg
     7501 gatattcttg cccccttatg ttgcttaatc atgacactgt tagccttttg gctggtatgg
     7561 ggagaataaa tagaaatggc attctattta ccctatctac tcatttttgc cagtatttta
     7621 ggaattattt ggcttagcta taaggcttat caaacaagat attcattaaa aggctctaaa
     7681 attcgattta agaggttttt attgccaggg gttatattta cattatcaat cgttttaagt
     7741 ggtttattcg gcgttttaga gggaaataaa cgtatttcaa gaagtatttt attaggggat
     7801 atcactcaaa agtatgaagc agcatgtgat aaaaagaaaa aggagcaagt gttaacacaa
     7861 aaaatggaag agtttaccac ttgttatgag gaaatgaacg atatttttgt gaatcaagaa
     7921 aaacgtttga cggataaaaa catggaaaaa ctcactcgtc tttatcaaaa cttgccagaa
     7981 aaacagcaaa aagaggttca agacaactat gagcgaacca aaaaagacgt gcaatatttt
     8041 aaagatacgc agacagaaga agcttgttat aatttattca gtgatacaat ccctttttca
     8101 actagtgaac aggaacgaaa agaacggcaa caatcagtta cctacgaacg ttataaagca
     8161 ctgcttcagc aagcaacgaa catacaaaat ccaacgaaaa aagaaacggc attaaattat
     8221 ctaaaaagtg tcaaggaatg gttagatcag caacagcaaa attgaatttg atcggcaatc
     8281 atgttaaact aaacatgcaa atgatcaaac attagataca cgaaagcccc agagaggttg
     8341 gattctctgg ggcttttttt caggttgatg tcgcctttat tgattttact tcttgtttcg
     8401 gttattcaac caataactga ataaagagac acaaacttct ataagaaggg ggcaaatgaa
     8461 caattcaaac attagataca cctccttcca gtcgcaagac tgtcggatag gcgacaagtt
     8521 attatatctt aaaaaattaa acttgtgtgg tgtttttaag tatcgcttat tttagggttg
     8581 ttagggcttg ccctgaccgc ctgtaaggcg ctttaaagct agataaggat ttttatccaa
     8641 gactgtctta aaacggctta gataagcatt cagaaccgct caaaggcctc tttaccaaca
     8701 tgtttttagc aaaggagcct ttaggcgact gcgcagtaca atgtgagagc gtagcgatca
     8761 cacctttttg gcgaccgtag gaagcaaggt tttggattgg cagttttgaa aaatgcgcga
     8821 acctgtttaa agaaaaaaga agtggttttt aggcgggaaa attagccccc ctctttttat
     8881 atctcttttt aaacctcttt ttaaacctct ttaccgtaag ctgtgtcctt actctaccaa
     8941 tggatacaga atgttatgaa ggacattttg tctgtttatg aaggacattt tgtctgttta
     9001 tgaaggacat tttgtctgtt tatgaaggac attttgtctg tttgaacgtc aaatatgaat
     9061 gtcttgacat ttaaaaaatg tcattcataa ttggattgag gtgattatat ggctaatgag
     9121 attgtaaaat accaccatga attaaataca attcctttaa gaaaatttac accggttgaa
     9181 atgaatttgt ttttttccat cgtgtctaga atgcgtgaaa aaggggataa gacagttcgt
     9241 tttacgtttg accaactaaa agatttaagt aactacaaag caactgcaaa tgtacgattt
     9301 gtagaagatt taaaagcaac ttatgaaaaa atcttatctt tacgctttgg tcgtagaagt
     9361 gagtctgggc tttcttacga tatgtttgta atgtttacag aatttaaaat caacggggac
     9421 gtagaaaatc cttacgtaga tatacgaatt tacgaaaaag cactgccttt gttaaataat
     9481 ttagacgagt gggtacgcta ttctttacaa caattcaacg agctacaaag tggctatgca
     9541 aagactatgt ttcgtctgtt aaagcaatac agaaccaaag gtttcgcata tttctccaaa
     9601 gaagactttc ttgaattatt agatattcct aaaagttata agcaacctga tatagataaa
     9661 cgagttataa aacctatacg tcaagaacta actgcaatct tcaaaggact aacaatcaaa
     9721 aagaaatacg gaaaaggacg gggaaaacct gttattggct atcaatttac gttcaaacct
     9781 gagatcaaaa aagcagacga ctttaacaaa catgcaccaa ccaaagaaga ccaaaataaa
     9841 gcattggctt tagctgataa acaaaaagaa tacgaagaac ccgctacaac aattaacgaa
     9901 aaaatcaaac agaagaaacg agaacttgaa ctcctccaac aaatcaaaca actagaaaaa
     9961 gagaaaaaag ctcttgaaaa acaaacagag tccgcacttt cggaaaaaga gaaaaaaagt
    10021 ttattatctg atttagataa tttattccag tagcaaccta ggaggcaacc agtatgcaac
    10081 cacaagaaac ctttacaata aaggaattgt cagatctttt taagatgagt aggcaagcaa
    10141 tatcgaaaca tatccaaaaa ttagattctt ccatgatagc taaaaatgag cgtggttata
    10201 aggttgtatt gcgttctgga gtattgcaac ttgctcgcaa cttagacaat caaaggttgc
    10261 tagataagtt gcataaacat gaacaaaaca acgataaaaa tgcttatata ccaaacaaaa
    10321 acgtggttgc aaacaagttg ctagatcagt tgcgtgaaga aaatgaattt ttacgagaac
    10381 aacttgccct caaagagcaa aataattctc atatacaaaa actactcgac cagcaacaac
    10441 aactcaccct gcaagccaac caacaaatcg aaaaacttca ggagcaactg cagttgactt
    10501 atacggaaga gtcttcagag aatagttcaa cgactctttc tgaagcaaca gaaacaatgg
    10561 acgaacaaac aaagaattgg aaaaaatggt ggcagctatg gaaatgaaga aggaaatact
    10621 tatgacaaaa aaacacttgc tattcggttc tatcctacta ggaatattaa gctttagccc
    10681 aatgttatta tcagtggcca acaaaggaaa taaaaaaggg gaactaaaac tcaatcctaa
    10741 ttactacaac agtcaaaatg aggaagtatt tgtagaaaaa cttgataaat aagaaaagac
    10801 tgctcgtacg agcagtcttt ctgaaagaga ttatcctatt attaacctta gaaaagaata
    10861 ataaatgaat atgagatatg taaaaagcat aacaaacaca aacaaaaata acaagtattt
    10921 aaaaaatgac cgatcaatat tcacaaagaa ttagaaagaa acaaccgcag aaaaccaagc
    10981 cttaaaaaga gcttttaacc tagaccaata aaaattcaca aaatcagcgc ctggatccct
    11041 tggtacaggc gttacttggt agtatcgaca ttatgtctac tagctgttta aataactgat
    11101 cttataattt tatttaaacg attatgaatt caaaactact ttttatgacg ttaatcattg
    11161 ttatataata aaatgttata taataaaatt actaagatta tttaatataa caagcaattt
    11221 taaaaaaacg aaaaggaaaa acaatcatga aaaagaaagt attaaaacat tgtgttattc
    11281 taggaatatt aggaacttgt ctagctggca tcggtacagg aataaaagtt gatgcagcta
    11341 cttactatgg aaatggtctt tattgtaaca aagaaaaatg ttgggtagat tggaatcaag
    11401 ctaaaggaga aattggaaaa attattgtta atggttgggt taatcatggt ccatgggcac
    11461 ctagaaggta gtggtaggga ggttattata atggatttta ctaaagaaga aaaactttta
    11521 aatgcaatta gtaaagtata caatgaagca actatagatg actatcctga cttaaaagaa
    11581 aagctctttc tttattctaa agaaatcagt gagggaaaaa gtgttggtga agttagtatg
    11641 aaattaagta gttttcttgg aagatatatt ttaaaacata aatttggatt acctaaatct
    11701 ttaatagaat tacaagaaat tgttagtaag gaatctcaag tatatagagg atgggcttct
    11761 attggtattt ggagttaatt ctttggaata aaatgaaaat gggactgaag aatcaattct
    11821 gattcatcag tcccataaat tatagagccg aaaaattatc atatttatag atttattaat
    11881 agtaaatggc ataagaatct ttgaacaacg aaaaaaatgt agctctacct aattattgat
    11941 ttgcaacaat ttcaccgaag gtgaaacaag cgaaacttta caa
//
LOCUS       DACJGB010000084        11788 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.086, whole genome shotgun sequence.
ACCESSION   DACJGB010000084 DACJGB010000000
VERSION     DACJGB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.086"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            514..1077
                     /locus_tag="IYI34_002508"
     CDS             514..1077
                     /locus_tag="IYI34_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560341.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1138..1503)
                     /locus_tag="IYI34_002509"
     CDS             complement(1138..1503)
                     /locus_tag="IYI34_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560342.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1579..1824)
                     /locus_tag="IYI34_002510"
     CDS             complement(1579..1824)
                     /locus_tag="IYI34_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560343.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1894..2148)
                     /locus_tag="IYI34_002511"
     CDS             complement(1894..2148)
                     /locus_tag="IYI34_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560344.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2312..2635)
                     /locus_tag="IYI34_002512"
     CDS             complement(2312..2635)
                     /locus_tag="IYI34_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560345.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2822..2895
                     /locus_tag="IYI34_002513"
     tRNA            2822..2895
                     /locus_tag="IYI34_002513"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2856..2858,aa:Ile,seq:cat)
     gene            complement(3280..3777)
                     /locus_tag="IYI34_002514"
     CDS             complement(3280..3777)
                     /locus_tag="IYI34_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAR0560346.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3817..4626)
                     /locus_tag="IYI34_002515"
     CDS             complement(3817..4626)
                     /locus_tag="IYI34_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0560347.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4960..5817
                     /locus_tag="IYI34_002516"
     CDS             4960..5817
                     /locus_tag="IYI34_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAR0560348.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5810..6712
                     /locus_tag="IYI34_002517"
     CDS             5810..6712
                     /locus_tag="IYI34_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAR0560349.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6745..7095
                     /locus_tag="IYI34_002518"
     CDS             6745..7095
                     /locus_tag="IYI34_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAR0560350.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7129..8067
                     /locus_tag="IYI34_002519"
     CDS             7129..8067
                     /locus_tag="IYI34_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAR0560351.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8145..9038
                     /locus_tag="IYI34_002520"
     CDS             8145..9038
                     /locus_tag="IYI34_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0560352.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9076..9639
                     /gene="amaP"
                     /locus_tag="IYI34_002521"
     CDS             9076..9639
                     /gene="amaP"
                     /locus_tag="IYI34_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAR0560353.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9653..9841
                     /locus_tag="IYI34_002522"
     CDS             9653..9841
                     /locus_tag="IYI34_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAR0560354.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9854..10756
                     /locus_tag="IYI34_002523"
     CDS             9854..10756
                     /locus_tag="IYI34_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR0560355.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10788..11030
                     /locus_tag="IYI34_002524"
     CDS             10788..11030
                     /locus_tag="IYI34_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR0560356.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11286..11657)
                     /locus_tag="IYI34_002525"
     CDS             complement(11286..11657)
                     /locus_tag="IYI34_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560357.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4180 a         1985 c         2233 g         3390 t
ORIGIN      
        1 aaaaattcat tggtcgttga atgggctgaa tagtagtgta taaaacaatt ttgtggagcg
       61 tgaagctttt atggtagagg aaaatacgaa taaagtgatg ttgataaaaa gattcctatc
      121 ttgaatatga atcgagatgt cttgaaaatg aatgagtttg accaatcatt atcctatcga
      181 aatttacagt ttcgttcgat tggtctgtca attaatgaaa aagtaaaaaa gaatatgttc
      241 taggaagagg gcggcacatc taaattttta agtaacggac agttcaaata tcaagcattg
      301 aaagaggcta tcctactgtt tgaaggaata cgaatatata aaaacgacaa agaggaaaaa
      361 gtagttcatt aaatcccttc aagtgatgaa agaatgagat ctaataaaac ctgatctttc
      421 atctgttttt ttaataacgt aataattttt ttattttttg tgctttcgcc gttattgtag
      481 agtgatttag cattcttatt tttaaaaata actgtgttat atattaaaaa aggcataata
      541 gttataagct tttttaaaga atatttagaa attattaatt ttcgtctgaa aacaaaaaaa
      601 gaaagcgtta ttattttagt ctattttcag ggggggaacc ttatgataaa aaaactattt
      661 ttactatcaa tattaacgct agcaatggca ggttgttctg actctggctt agcaactgaa
      721 ctgatcacaa aatcaactga agccacgaca gaggagttaa aatgtggaaa cgatcacagt
      781 gactacagag gcttaaatcg acgatgcatg ggaatattag gcagaagcaa ttgtaagcat
      841 cgatgtagta atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag aaagatcaaa
      901 agaagcaatc tgtgctgtgc atcgtataca gacgtcgaag atgcggtact tttaaaccac
      961 gtcaggagat gttcatttat gtctagggaa gttggcgatg aaccaaaact agagtacgct
     1021 tttagttttg cacgtgagga tgaagataac cctaaaactt acgacatttt agcttgatct
     1081 aagatggtta aagcttttta atgaagtaag aggagataga aggcttttct agttatatta
     1141 ggtaagataa attccttttt gctccaaatt tgctaaacat tctgctagat aaacttcatc
     1201 gtgttccgga taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa atgcttcgca
     1261 tgatttgcca cgatacatag gatttttcca ctctggtgct gggctgtaac cacgtttttc
     1321 catttcatcc atgacaagct gatgatattg gaataatcta taaggagaat agtgaaaaac
     1381 ataatccaca gtagcatgtt tctttcccca gccatttcct cttaatgcac agcattcccg
     1441 atgttgtcct aaaagctgtt gacgaggaag ctttgaaatc aatttttcat gccataatcg
     1501 catagaaatg tctcctttat atatagattg ttaaccttag acgttaaatg aaaaaaccag
     1561 caccaaggct ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac caattaggta
     1621 atcaacggaa atatcgtaaa agtcagcgat atagattaat tcaatgatgc ttggaatttc
     1681 ttctccttct tcgtacttac gatatttgaa taaaggaata cttaacgcgc tagaaacagc
     1741 tacttgagat aaagatcggc cagttcttaa gaaattcaga cgacaacgta aagtatccag
     1801 taaaatagct tcttcgatac tcatttaatc acctttcatt atagtagaat agttataatg
     1861 actatagaaa gctacacatg caatcaatag tactcaatgt tttttattta aacgtatcaa
     1921 ataaagaatg acgatcaata aagacaaaac cagtcctgat aattcttcta taaaaacact
     1981 atcgggttca aagtagttgt aattaaaaac tttccacata aagtagatag ccggaagagt
     2041 caaaatagct aaataaaacc gttctttgtt ccaattcata agataaacaa tacttgcgac
     2101 cagtgcgacc gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt aacggactcc
     2161 tcaacgattc atataataaa agataacatg ttattgcata catcacaaat gaaatgctag
     2221 gaaaataaaa gaagaataga tcaatgaaaa atgaaaaagc agaacagttt tctgctgata
     2281 agcaggaact gttccgctga atgtgtctta tctatcgaga aaaaatcaat ggattgatat
     2341 ctaagtaatt caatacatat aaaaagagga cgatcgcatt cactaaaaag gaaagaatga
     2401 tgatcccata gcgaatggaa acatatttaa caaatacttt tgtttgaata cgggcatatc
     2461 tttttccttt atagctgagc atcatgacaa gaagcaaaag ggtaatcaca ccttgaaaaa
     2521 tcattccaat aaacagaatt cctgaggttt gataatttaa ttggagaaaa taagctgctg
     2581 ccgcaactaa atcgaataaa atcgcaataa atggcaaaat aatcactcct aacattgaga
     2641 ataaataaaa gaataacatt ttttctaaaa taagcaaata aaaattaaag taacaaaata
     2701 gttatgacac aagtaaaaga aataagaata acaatattat tttacttata ttttgaaact
     2761 tgtgagagtt tgtcattcgt gctatagttg aaaagtaaca aaaataaatt ttatttatga
     2821 tgtaccttta gctcagttgg ttagagcaga cggctcataa ccgtccggtc gtaggttcga
     2881 gtcctacaag gtacataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     2941 ccaagtaaaa acagcttctt atgtttttac ttggctgttc ttattactgc agatgcaagc
     3001 ttttgaatac cgtttaagga agaggttgtg aaatcagcgt tttgacctga gtattagctt
     3061 aacaaacgga aaaatagttc ctcatatttt gccgtttgtt aagctaatac cgagggtcag
     3121 ctgattgaac accatttaag aaagaggtcg tgagcctgcc gtttcactct gtgtcataaa
     3181 aaagccaatc tactttgata gaaaaagcac gtaggactaa aacagttctg cgtgcttttt
     3241 ttattgtgtg ttaaatatcg taaatgaaga tagatatttt tatgattcga cgatagagac
     3301 gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt tttctactgg agactaacat
     3361 tggatcaaca aaaaagtctg cttcaggaaa agcactctgc aggcctatca gattagagag
     3421 tacacacaag tttgtttcaa tcccgactac ttcgatctct ttttgttctt ctttgctttc
     3481 aaataaagtt tcctggattt cagctaaagt ttcaggtgga atcgtaaagt aatacttttt
     3541 aatcactcgt tcattttctt gtggagaaag aagagggatc aattgcaaat cttctcgttc
     3601 ttcttctttg tccttcagtt caactggtat atcccgtgtg aagagaatat attcattatt
     3661 ttggcgtgct ttagctaatc tttgagcaat acgttctgcc aattgatctg actctggaat
     3721 ataaaattcg ctgtttggat caagaatatg attttgcata tcaataataa ctaacatttt
     3781 ctttcctcct acattcatat cgtcatttta ttctttttaa tagaagtttt ttaaaatata
     3841 agcaacacca gcttcttcat tcgtcagtgt tacttggttt gcaatttctt ttacttgtcg
     3901 ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc aacatagcaa tgtcgttttc
     3961 ttgatcgcca aaagccatga tgtcactcga ttcgattcca aggtaatccg ccagaaaaac
     4021 aagtgaatga gctttagtga tacctgtagg cgtgatttct aagaaatgcg gatcagagaa
     4081 tactgattgg ataccatctg cttgcagatc tctttgtagc ttctgtagtt tttcttcact
     4141 cttatctgtt aatgctaatt taagatagct aagagaagga tcttccaaca tatttaaaat
     4201 ttgatcaaaa gaatggttct gtaaattgaa atgttcataa taagggtcat ctttgactag
     4261 atttgattca tcgaatgtca gctgatcatc ttgggtttct cggctagcaa tgatggatat
     4321 tttcgcttct tgagcataga agaaggcttt tctcagtgta tcgctatcta tttttgaacg
     4381 tctgagaact ttcgccattt ttccatcctt catttcagca atcagtgaac cattggaaga
     4441 aatcaaatag ccaccgattc ctaattcgga aaaaatatct ttagcagaaa tgctgttcct
     4501 tccggtagcg acggttacga ttccgccatt ttcatcaaat ttccgaagtg cttggatatt
     4561 ttcttctggt atctttccat ttcgatcaag caaggtaccg tctaaatcaa tcgctgctag
     4621 tttcatttta tcttcctact ttctttttac atatctattt tcctgttacc ttaagattaa
     4681 atgagaattt aaaaaaatga aactaacttg ctttgtcatt atgaattttt tcaaaaaaca
     4741 ttcttaagaa aaaggtggct atatatagta gtttttggtc aatacctttg aataaaaagc
     4801 catgaaaaac gaatagttag cgcttaccga tgcgttttgt gataaaaaaa ttttaaaata
     4861 ttcaatgaaa acaaagcata tattttgctt taaaaccaaa tgaggaatat tctagatgta
     4921 gaacaggtta gaaaaagatt agaaaaagga gtgattttga tggagaagag atacggcgta
     4981 gttcctgtgg taactgcttc agacgaaaat tatgctcctt acttgagcgt gatgatcgca
     5041 actgcattag aaaattgcaa taaaacaaga cgcataaaat tttatgtgat tgacgatggt
     5101 ctatcagaat atagtaaaga aggattggaa gaaacggtaa ataaatattc aagtaatgca
     5161 agtatccaat tcctgacagt ggaaaaagat atctacgaag acttcctagt cagtgaccat
     5221 atcacaacta ccgcttactt aagaatttct ttaccaaact tattagctaa agaggattat
     5281 aagaaagtcc tttacttaga ttcagatgtg ttagtactag acgatatcgt aaaactatat
     5341 gatgagccgt taaatggaaa aacaatcggc gctattatcg atccaggcca agtgaaagct
     5401 ttggaaagat taggaatcga ttccgatgac ttgtacttca attcaggagt gatggtgatt
     5461 gacattgatc aatggaataa aaaagaaatt acagaaaaaa ctatccatta cctcagtgaa
     5521 aatggcgatc gaatcatcta tcacgaccaa gatgctttga atgctgtact ttatgaagat
     5581 tgggaacaac ttcatccaaa atggaacatg cagacttcgc tgatttttga acgtcatcca
     5641 gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc ttcgatcgtt
     5701 cactttacag gacatgataa accttggaat acgttgaaag atcatccgta taccaattta
     5761 tacttaaaaa aattagcaca cagtactcta acgaaagtag gcgagatcaa tgaataaaaa
     5821 agaaatagca gtagtcgcaa gttgtaacac aaaattcgta ccacatttag ctgcattatt
     5881 tgtctcagta ttagataatt gcaacccgtc taaatttgtt cggttctatg tgattgacga
     5941 tgatatcgat tttgaaagta aacagctttt acgcttctca gtgaaaaacg cacgaatgaa
     6001 ctcagatgtg gaattcttga aaatcaataa agaattcttt acaaatgtgg taatcagcga
     6061 tcgtattcca gaaacagctt attatcgtat tgcgattccg gaattgttcc gcggcacaga
     6121 agttgaacgt atcttatata tggattgcga catgatcgct ttacaagata tctctaaatt
     6181 atggcgtctt gacttcggtg attcaatcgt agcagctgtt gaagacgctg ggttccatca
     6241 acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact caggtttgat
     6301 gctgatcaat gtgaaaaaat ggttagatga aaatattacg caaaaagtat tagatttcat
     6361 cgaacataat ccagaaaaat tacgattcca tgaccaagat gcattaaatg ctatcttaca
     6421 tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa ggctatatca tggcaaaagc
     6481 aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa gaaactcgca acaatcctta
     6541 cattatccat ttttcaggac atgtgaaacc gtggagcaag gattttgaag ggccaacgaa
     6601 gaaatattac gaaaaatatg cagggatgac ggctttccgt tgtgtagcta aattccctaa
     6661 atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat aatagaagac
     6721 aacaactgaa gagggggagt aggaatggaa attacaatgt ctaaaaatgc agtagagacg
     6781 ttaattgaaa aggttgggga aaatacaaaa atcgcactcg cgcttatcaa tgattccgat
     6841 ccatttctaa gggacaaagg tgcttttgca aaaggcagct tttttcaaat cattcctttt
     6901 gtttcggaat ttggagaata tgcgactaaa atcgagcatc cattattgga catctataca
     6961 tctaaattag agcaaaatta tttcggtaaa cgattgaaca tggacttcaa caaacagctt
     7021 gacagctttt cattagaaaa tgaaatcgca gtactcgatt ataatatcaa attaaagaac
     7081 tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt gagatactac
     7141 tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa ttggctcgta
     7201 cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga gcttcctgat
     7261 gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg cctttatgag
     7321 acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc agataatcgt
     7381 atcgaaaaaa aattaacgcc aatatctttt gtactttccg ataatctctt gattacttgt
     7441 atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa tcatttaaac
     7501 tcgtttgaaa agatcatcac gtatacatta ttagatattt acacccatta cgtgaaagaa
     7561 ctaagagaga tgaaaaagcg aattgacgct ttggataaag cagctcgtaa aacaacggaa
     7621 aatgaagaac tgtataaaca ggcagatctt gaaagagaca tcgtatatat cgatcatacc
     7681 ttaagagatc aacgggaaac aatggatcaa ttatggaatt ctgaaacttt catcaaacgt
     7741 ttagacgatg aacagctact ctatgatgtc catttgagac agcgacaaac agaaaaaatg
     7801 atcgagatct atcgagattt attagagagt attggtgatc tattcaacgg gatgatggac
     7861 aataatctaa atcatatcat gaaatatcta gattcagctg cattaatcat ttccgttccg
     7921 gcgttgttcg cagggatttg gggaatgaat acaggaggat taccgggtaa aagttctgct
     7981 ctaggcttta tactcgtagt ctttggctct ttgatagcag cagtagtttt aggttaccat
     8041 ttataccgga aagattatac aaattaaaga atctatttaa aacaggtatc aaacaaagaa
     8101 atttgttttg gcaatacaat ttaaaaacta ggaggaatac agcaatgaca gaaccaaaat
     8161 taatcgaccc gcgaaacttg taccatacgg agaaatttcc taaacaagat caggatacac
     8221 cagctttaca gcagaatatg attcctgttc ctgattgtgg agaagaatcc tatgaaggca
     8281 ataatcaact agaaaaccgc cgtgtactaa tcactggtgg agattcaggg atcggtcgtg
     8341 cagcagcgat tgcatttgct agagaagggg cagatatcgc tcttcaattc ttcccaggag
     8401 aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaagt tgcaggacgt aagattgtct
     8461 tactaccttt tgatcttcgt gacgaacaag cacctaaaga aatcatagat aaagccgtga
     8521 gtgaacttgg cgggctagat acgatggtac taaacgcagg acagcaaatt tcttgtcagt
     8581 ctattgctga gttaccactt gagcaagtca aagacacatt tatgataaac atcatcgcta
     8641 tgtttgcttt agtgaaagaa gctgttccgc atatcccagc aggaggagca atcgtaacaa
     8701 caacttctgt tcaagcattc aatccaagcg aacatttatt agattacgca gcaacaaaag
     8761 cttcaatcgc taactttacg atcggtttag caaaacagct tgcacctaaa ggaatccgtg
     8821 taaatggtgt agcacctggt ccaatctgga caccattaca actagacgaa ggacaacctt
     8881 ctgattcgat ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt cagccaggag
     8941 aattagcacc agtttatgtt ttcttagcat cgaataaagc aagttatgtt acagcccaaa
     9001 tttacggagt aactggtgga gaagcgatca acttataaga agttgatcgt ttcaccccta
     9061 tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg aacattactt
     9121 ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt gccttttcag
     9181 ctagagaagt ttcgattctt ttccatcact aattcatacg tgcaacaata tgtattttgg
     9241 cttgccgttg catttgctat tttgacaatt atcacgttac ttgttttcct gttttatcca
     9301 aaaacaatcg gaaactttct actgaaacat ggtgatgggg agttgacgtt ggataaaaaa
     9361 gcaattgaag gaatggttcg ttctcacttg catgaagatg aattcatcca ttcaccaaaa
     9421 gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg taaaagggga gttgaaacga
     9481 acttcagcac tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa tgaagtacaa
     9541 cacatattgg gaacaaaaga gccagtgaaa gtatcggtga aatacacagg ctttgaagaa
     9601 ccagctaaac gaagcaatga ccgcaaacga gtagaatagg gggggaccaa aaatgaaaga
     9661 actattttcg acatacaagc tccctatcat ctttggggta cttggattga tattagccgt
     9721 tttatggatc accgtgggct ttttcaagac cttattgatc ttgatcatgg cagcgatcgg
     9781 cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta gatcactatt ttaaatctta
     9841 ggaggaaaca tccatgaata acaataacaa aaataataac aataaaatga acaaccaaaa
     9901 accagtgtct gcacatccag acccaacgac accaaaccaa tcaaatggtg tgaacgatga
     9961 aaagaacaaa acagcaccag tatctgcaca tccagatcca gtagtcccta acgttcaaca
    10021 aggagtagaa gagaagaaac atgatgcaga aaagcatgca cctcatcatc caggaactcc
    10081 agaatctaaa gttccagaac aacatgatgc aatcgatcct tctaaacctg tggaagagca
    10141 aaaacatcct tctgaccatg cgaaagcaca agaaaaactt agttctgacc acacaaacgg
    10201 acaaggaaaa cttggttctg atcatacaaa taaccaagga aagcatgatt ctgaacagga
    10261 agattcaatg cgcggtgagc tgaattatga agataaagtt gtgcaaaaaa tcatcggaat
    10321 cgcaattgaa caagtagacg gtttattatc tgcaaacggt ggtttcttct ctaatgtggc
    10381 aggaaaactt gtgaatacag ataatgtaac ggctgggatc gaaacagaag tcggtaaaaa
    10441 acaagtagca gttgatatgg atgtcattgt ggaatatggc aaagatatcg aaaaaatctt
    10501 tgaagaaatg caagaagtaa tcggcaaaga agtaaaaaat atgacacatt tagaagtgat
    10561 cgaagtcaac gctaacgtag tggacatcaa aacaaaagaa gaatttgaaa aagatcaaga
    10621 aacggtgcaa gacaaagtaa ctgacgcagc gaaaaaaacc ggtgaatttg cttcgaatca
    10681 aacagataaa gtaaaatcag cagctggaag cggcgcacaa aaagtaaaag aaaatacaga
    10741 accacgggtt cagtaataag gaagtaaaac attaggagga acgaaacatg ggatttattt
    10801 ggtcacttat cgtcggagga attatcggag caattgcagg agcaatcacg aataaaggtt
    10861 cttcgatggg gattattgca aatgtcgtag caggtttagt tggatcagca cttggtcaag
    10921 cattattagg ttcttgggga ccaagtattg caggaatggc aatcgtgcca tcattgattg
    10981 gtgccatcat tttagtacta atcgtatcat tctttgctcg aaaactgtaa aaagactttt
    11041 aaaaagaggt cgtgaaaaag ctgttcttca tcaagtaata agaacagcca aacaaaaata
    11101 gcttctcata tttttcattt ggttgggcat attatcgaag atgcaagctt ttgaacactg
    11161 tttatacgga ataagaggct gtgacaagac ttttgtcaca gcctcttatt tatgcgggca
    11221 aattcccaat tagattttta aaagtttttc cttaaatgtg tctttattga tacagagaag
    11281 cttctttaat aaaacaatgc ctccctaaat tttcttgaga taggaaaggt gaacatacaa
    11341 aataatacga ccactttgaa aacccagatg atccacaaat ggatcgtatg atactgatga
    11401 agtaaaaagc aatggatcag aaactcaaag agattgaaaa agacaaggaa gaaataatat
    11461 ctctttttta ctcttgtctg ttttagccaa taatagctcg taaagtaatc tagacaaaca
    11521 aatagaagta aacaaagttt cgtaagagca gcaatcgtat catgttgctc tgctagacga
    11581 ttgacgagca ggaaaaatgt tggtaatgga atatagatca cgagtaatga aagaaaaaag
    11641 taaacaataa atggcatact attcctccta attgtgtgag ttcgccctca ttttaaaata
    11701 acgatatttg tttgtgaagc tttttcgtgt cgtaacaaat gttatctaat gtatttttaa
    11761 ataaataatc attattatca aaataatg
//
LOCUS       DACJGB010000085        11618 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.087, whole genome shotgun sequence.
ACCESSION   DACJGB010000085 DACJGB010000000
VERSION     DACJGB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.087"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            441..1046
                     /locus_tag="IYI34_002526"
     CDS             441..1046
                     /locus_tag="IYI34_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAR0560358.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1062..1634
                     /locus_tag="IYI34_002527"
     CDS             1062..1634
                     /locus_tag="IYI34_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560359.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1886..2143)
                     /locus_tag="IYI34_002528"
     CDS             complement(1886..2143)
                     /locus_tag="IYI34_002528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560360.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2269..3084)
                     /locus_tag="IYI34_002529"
     CDS             complement(2269..3084)
                     /locus_tag="IYI34_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560361.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3601..4926
                     /locus_tag="IYI34_002530"
     CDS             3601..4926
                     /locus_tag="IYI34_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAR0560362.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            4919..5269
                     /locus_tag="IYI34_002531"
     CDS             4919..5269
                     /locus_tag="IYI34_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560363.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            complement(6340..6615)
                     /locus_tag="IYI34_002532"
     CDS             complement(6340..6615)
                     /locus_tag="IYI34_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAR0560364.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6587..7540)
                     /locus_tag="IYI34_002533"
     CDS             complement(6587..7540)
                     /locus_tag="IYI34_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR0560365.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8152..9645
                     /locus_tag="IYI34_002534"
     CDS             8152..9645
                     /locus_tag="IYI34_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAR0560366.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9780..10076
                     /locus_tag="IYI34_002535"
     CDS             9780..10076
                     /locus_tag="IYI34_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAR0560367.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10177..11028
                     /locus_tag="IYI34_002536"
     CDS             10177..11028
                     /locus_tag="IYI34_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560368.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11049..11261
                     /locus_tag="IYI34_002537"
     CDS             11049..11261
                     /locus_tag="IYI34_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560369.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4015 a         1790 c         2148 g         3665 t
ORIGIN      
        1 agaggctgca aaaaaagatt taattgatat tcttagataa aatcagaatg acaagaatat
       61 ttacgttcta gattctaaaa taaaacacaa agagaccaac caggcaccct attgtttctt
      121 tgtagtatag agatattctt tggcttgtaa gagatcaata gagttaggtg accggagttc
      181 gaaataaagg aaatttttga ttgtatagga gaattcttta tccctaatat ttagaatttt
      241 gagtatgcca tgttgatgtt ttagattctc tataaatata gaggtgtatc aaaggtcaac
      301 tagactgtgc aaaatagaga cacttgaaaa atctatcggg ggtcagaaac ttaatagttt
      361 ttgacacata aacaaaatcg cgatataatg gggttgagag tgtttcataa acttatgtca
      421 taaagttaag gagatttcgc atgattttag agaataaatt gggattaacc aatcaagttg
      481 agttggctaa ggttgaagaa aaactcagta aacaaaaagc gaaagaattg tacgattgtg
      541 gaaaaataaa tgaaatcgaa gttggtacat ttaaaggttt atctgaaatt catgaatttc
      601 tattttcgga tatttacgat tttgccggaa aaatccgatc ggtcaatatt gcaaaaggaa
      661 attttcgatt tgcaccagtt atgtatttag aacattcgtt acagcatatt gatcagatgc
      721 cacaaacttc ttttgaagag attatcaaga aatatgtgga gatgaatatc gctcacccat
      781 ttcgagaagg aaatggccga agtactagaa tttggctgga tctcatttta aaggaagaat
      841 tgcaaaaagt tgtagattgg aacttaatcg ataaagcaga ctatctttct gcaatggagc
      901 gaagtcccat aaacgatttg gaaatacgct atctaatttc caatgctctg actgataaaa
      961 ttgacgatcg cgagttatat atgaaaggaa tcgatgtcag ctatttctat gaagggtatt
     1021 ctgagtatac gatagatgat ttgtaaaagt aggtggacca aatgacgaaa tatggatatg
     1081 cacgtgtctc aaccgcttct caaagtacaa aagaacaaat tcaacagttg cttgagaacg
     1141 gcgtagagaa gaagaatatt ttttcagaaa aatttacggg tacaaaacgg gatcggccaa
     1201 aattcaatca gctgttggct ggtctgaagc ctggagatga aatagttgtt acgaagcttg
     1261 atcgatttgc tcgtaacacg agagaagcat tagagatcat tgaaccttta ctcgatgata
     1321 acgtaacgat caaggttttg aatctaggaa cgattgaaaa tacgtccatg gggcgaatgg
     1381 tgacacggac gttgttgagt gtagcagaaa tggaacgtga tatgattgtg gaacgtaccc
     1441 aagaaggaaa aatgtttgca agaaagaata atcctaattt cagagaagga cgtccgaaag
     1501 caacaatcac accgaagaag aggcacgcat atgaactgct tacttcaggt aaatcgtata
     1561 aggaagttga agctataacc ggtttcagtc gcagtacgct ttttagaata aagaagaaaa
     1621 ttgaagaaag ttaaaaaaga agtctcagcg aaccagacag gtaaatactt aatgcgttct
     1681 agatatgagt ctattagcat gttgtgaaat ataatatttt tttatttact tgatttctgc
     1741 tcaaaaaatg tgtatggtaa ctgtaaaagg taaagtaatt aaaggtatgc tagccacgct
     1801 tactatatta ggataccatc atatatttat aatgaacgat ttgttcgtct accaaacaac
     1861 acatatcgtt cagcgtaagt cacgtttagg aacgtggctt ttttagtgtt attatcgata
     1921 aataatctat tgactctatt gattttatct ccatatccct acggtgttga ttgtctttag
     1981 atctttcatt taagaaccaa ataactatca taaaaatcag cactgctgct aatataataa
     2041 acagcattcc tattgtgata ttacctattg attttaccat aactacgatt aaagtaataa
     2101 tggcattcaa gtctcctttg ttctctatat ataaattagc cataaatcat cttcctttca
     2161 aattaagata cttgtttgat accaataaat agtaagcgtg gctagcatac ctttttctgt
     2221 aatagcaagt gttctaatag tccaaactta atgatggacc gatgctagtt atagtacagt
     2281 aatgttttct ttcttatcta gatttttagt aagctctatc agcaaatcta acaaatctga
     2341 gtccttttca gaaactaaat taaatctagt gtttacaaca aagttttcat gttctatatt
     2401 gtttagatta tttgcggaaa ggagggcttc tagatcttga cattcaaatc tatatgcatc
     2461 gtcataaatc ttactagaag gagtgcttaa ttcgaaatta cttattttct catcttggac
     2521 caagataaat ttatagatag tatctttttg tgtttttctt ttaaaaacac aacagagtgt
     2581 gttataagga acgttataat tttcatcagc ctcatttttt ctccattcaa acaaatgata
     2641 aactgcaacc aagttgccat caatttcatg gacagtgctc tcggtaacat cctttaaaga
     2701 actatcaagg gctaatttat agtaactaga gagaggaagt aaaattgtcg atttactgag
     2761 atggttatcg tctatacgtg caatcatacc taaatcaact aaggttttat tccaggtgtt
     2821 tatagtttta gttgtcactc ctagagactc tgctatggta ttgaccgaat gccaggaatc
     2881 cccgccttca ttctttgcct taaaacagta atacagatat aaattaatag ctttgttatt
     2941 cggtaaatat ttttcaagtt ctgttggtag aataaagtaa gaatccttaa tgttttttct
     3001 gaactgtctc cagtctctgt agttttgttt ataaagttgt gaaacggttt ttccaccttt
     3061 agaaagtcct gctatcttaa ccaatcttat tgctcctttc ttgaatctgt catgatcata
     3121 cagtattttt tacttgtagt ccagcacctt aatatattat cctatgtaat taatttcgta
     3181 ctagatagta attatttttt atttaattct ctagtcactt acaaataagt tatataaact
     3241 acgattaata aattattaca aaaactatta agaaaacttc tacatacaac tattacaaaa
     3301 ctattaagaa actgaatact cctatttttt gtttttaagg aaagtttttt cttttttgaa
     3361 tttcttattt ttttctaata taactaagca aaactggata atacgacact tattttcaaa
     3421 ttttcacaag actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac
     3481 aaatcaacag aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc
     3541 gattcttctt tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt
     3601 atgatggatt attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt
     3661 tacgcaagtg tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc
     3721 atgagtcgag cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag
     3781 aagaagtacg gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa
     3841 acgttacatg tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac
     3901 aatattttca gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg
     3961 atcttaaaag tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg
     4021 agaaagaagc tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct
     4081 acagtaggtg tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa
     4141 cataatgatg gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat
     4201 attcctgaaa tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa
     4261 attggtatca tgagtattaa agacttagct aattggaatc catacactat taagagccat
     4321 ttaggtgtga ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata
     4381 gcgatcccac cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga
     4441 gattatacca ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct
     4501 attcgtatca ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg
     4561 atcttagaaa cgagaccagg tttttcacac cagatgaaga tccctataac agataataca
     4621 aaggaattgc agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta
     4681 aggcacgttg gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta
     4741 tttagtgatc cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt
     4801 cggcaaaaat atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt
     4861 agtattacta gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat
     4921 gattgattat aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac
     4981 tgaaaaaatt gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat
     5041 gacgactgaa gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg
     5101 tattcaaaaa gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat
     5161 cttaggtagt gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat
     5221 acgtcatatc gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag
     5281 caatatgagc taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc
     5341 cagcgtttga taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca
     5401 ggataccata aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt
     5461 tatgttttac attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa
     5521 aaaaactagt tacaacctta ggacgttgta actagcttga agcatactag agctaactaa
     5581 aatagcacta gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag
     5641 cttattcagc taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac
     5701 tacgaataac gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac
     5761 taaacattgc gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag
     5821 ctttatgatg ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa
     5881 ttctcctttt taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc
     5941 atttgattga gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt
     6001 ataacataaa tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca
     6061 aacgatttat tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat
     6121 gtttgttaaa accaccatta cctgtagata aaaaccaata tatttactat actcactata
     6181 ttaaatatat ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc
     6241 ccaaacaata cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat
     6301 actaaatata tttactatat ctagtatact tactatattt taatcttttg atcgtctatt
     6361 atatataatc cgttcctcgt cgtttaaagt atcaacatag gcgtccattg caagtttgac
     6421 tatttcatac ataggaatat cttttgcata actaagatcc cgaatggttt ttagtacggg
     6481 aggatctact tgaactggtt ttctttcttt cgcagtgtac tttcgttccc ctaactcgac
     6541 attaatatca tctttaattg aaatatcttc agcaccaatt ttcttcttat ctttgttcgt
     6601 tggtaataat gccatttagt ttaatctcct ttccaagagg taggatatga ttggccattg
     6661 agtcagcata tttaggtgta taatcaaatc cttcaatatc accatgttgt tcatagtatt
     6721 caattctttc ttctaattct gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta
     6781 cataatcatt taattggata ccattctcac catacatttc taaccgatcc gagcctttaa
     6841 caatgctttt aaaaatgttt gattcaccat acttatcaat tgttttttga tagtttgcgt
     6901 gttgagagga acgtcgcact gagaaaagaa caggcaaaat tccaatgatt tgaagatgag
     6961 actcttcaaa attcgtgaca atcggaacta aaactttatt aataaaatct tctgttcctt
     7021 ccattgcgta ccgttttaat tcttgcattg caataatata atctgcagca gttaagaatg
     7081 cacgaaccac attatctgtt gatggtgggc agtcgataaa gatataatcg aaattttttc
     7141 tcaattcttc taaaggttta atgaaggcca atgatctctt acgacgatca ggatattttt
     7201 cggacaagta ttcattaagt tcttcagatc caggagagcc agccataata tataaatgtg
     7261 gacttacttg agtaatacct gaaacccagt cccctttttt gattgctgca gacatggatc
     7321 gatcaaagct agtaatttga tacgttgatc caatgttgga agtagtattt ctttgttggt
     7381 cataatcaat tagcaacacg tctttttcaa aaagttgact agaaacaatc gctaataaat
     7441 cattgtctgt agtcttacca atgccgcctt ttctacctgt aagcattaca acttttccta
     7501 gtttatctga tgttttaaat gcagtccgta agttttccat agttccctcc aatttcctat
     7561 actaaatata tttagtatac ttcatatact taatatattc gatatatcta atatactcca
     7621 aattgataag aattcagact ttataaggaa tatagtggat atactaaata tatttgctat
     7681 attcagtata tcagatatat tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa
     7741 tttagagtga aaagaggtaa acttaattct atagaaacca cagataattc actatttaca
     7801 aagtagcaaa agcagtggta ctctttaaac agaaaaatta cataaaaaaa agagacggaa
     7861 ataattgatt ttagcagaat caattatttc tgaatcacat ggttaggaca tgtgttcacg
     7921 ctcgtctcaa gaatatgtaa gtagctacca actacttata ttaaaagttg atcggatacc
     7981 ttttccgatt gcctttattc taccaaaaaa aggcgagcaa ctcaacagaa gaacgtttat
     8041 ttagtgtact taaattaaat attcttaaac actgaagaga gaacacatac tatggctgag
     8101 attaaagcta aactgtgttc tctttttgtt tgagaaagga cgttacaatc gatgttgagc
     8161 gaaatttatt cagctattct taaaaacggc ttacgtacat acaaatatcg aaacagcacc
     8221 atgaaacctg caggtcatat tgattccagt aggagtggtg ctatctttgc gtaccgtagc
     8281 aagcaattaa tgaatgttgg gcgtggtatg gtcattactt cggaagaagc tattttagag
     8341 aacgaaaaga aactaacgca ttggacacct aatacctatc gctttggtac ttatgcggat
     8401 gattatcgaa cagtggttaa aggtcattct gagaaaaact taagtcaaat caacacattt
     8461 gtagttgata tcgattcaaa agagaaccat caaggcgaaa ttattttagc ctgtttagat
     8521 caagtgggct acatgccaac gctaatttta gaatcagatc atggttatca agtttatttt
     8581 gtactaaaga caccaggcta tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca
     8641 aaaaaaattt caaaaacaat tcgtctacag ttggctgaaa aattgtccgg agtggatcta
     8701 ggatgtaacc acttcggaat tgctcgattc ccacaagaaa ataatattgt ttttaaagaa
     8761 atgaactatc agtattcttt tgctgactgg attcaatggt cgatgaagct tgaatcagat
     8821 caaaaaacaa aagaagatcg agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt
     8881 cgtcaagtag atgaaccgtg gttcgatatg ttacttcata aagcaaacat cgtcggcgga
     8941 gaaggacgtc ttggtcgaaa taatgtgatt ttcacgcttt ctttagctta ttactcttca
     9001 ggttatgcat tggatacttg cgaatacaat atgttcacat tcaacgaacg gttacaggaa
     9061 ccgctatctg aagcagaatt agtaaagatt gtacgtagtg cttattctgg taattatcaa
     9121 gcagcaagca gagaattcat tattgaattg tgccagcaat gggtttcacc aaccttaaaa
     9181 aataatgatc tgttcattca acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa
     9241 agaaaatata gtcataaaaa tgaatggaaa gaagatctat tggcttatct aagcgaaaaa
     9301 agtcttgcta gtgagcctta tttgaacctg tcaaaaaaag aattggcttc acaactaacg
     9361 atgcccaagc gaacacttga taagatctta aatgagttac gagaggagaa taaagtcttc
     9421 tacaaggtaa cacgaggaag aaatggcgga atgatcattg cttcagtcaa aacgttgttc
     9481 gcgagggtta ttcaactaag taaagaaaag agactagatt attcaacaag gatcacagag
     9541 gtatttggac tcaacgagag cttagtagaa gagacattta gacaattatc aacccccttg
     9601 aaccctctta aagaagtgaa tctttttgaa gtagatacag gttgaaaaat aaaaggtgca
     9661 aattgcctta tacaatatat ctatgatact tctgtaggtg ttgctccctt tgcctagcta
     9721 cgtgtaggtg gtatacttat aactgcgtat gcagtcttat gtatcatgga ggaagttgta
     9781 tgtcgttaaa aaattatgtc tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg
     9841 gattatctgt ggaagtgttc tgtgagcttt atggttttaa gcaggggact ttgtcttctt
     9901 gggttaccag ggaaaaaaca gttgcttctt tgccgattga gtttcttcat gggttgtcct
     9961 tagcttctgg ggagactatg gatcaggtat atgattgttt gtgtgtgtta gagcaggagt
    10021 atattgagta taaaatagct aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt
    10081 ttttatttac ttaaaaaatt ggttcattga gcaacctatc gctgctaaag tagcaaagga
    10141 aaacgttaaa ggaaagaatt tagggaagaa atcgaaatga aatcggacga ctggattgaa
    10201 cacacctggc ttcctctgaa aagaaatagc aaacgaaaga aattttgggt attgcaatta
    10261 tggccattag gaaaactttg tagttacggg gcacgactta ttcttgattt aaccgtttac
    10321 tcgattggag tatttggaac ggcttattac ttgataaatg atcccaatag gattgcagca
    10381 tcagacattt ttacagtgct aatgacgttg atttttgtca tgctattgtc tatttttact
    10441 caattagtca tgcaatttac caggcaaaaa ttaacagcag gaaaactttt caaaagccta
    10501 ttcgatttaa ttgttgtaaa tgcaattcta gcgactttaa taaaacaaat tgaacaatct
    10561 ggaagaacaa caaaaggagt gaaggatttt ttattgacgt ttattcactt tgtaaatgga
    10621 cacatgtatt tgtttgaagt gggtattatt ctcttttttg tcgggtatgt atccacgaaa
    10681 cttattaaac gttcaattga aaaagaaatt gaaattgacc tgattccgac acttcttggt
    10741 tatgagtaca atgttgattt tggaagatat gacgaattga tcaatacgga tatcgttcac
    10801 acgagtatcc atgaatctac tgttaagggc atcagatcta ttttaaaaaa tggccaggaa
    10861 atgaaagcaa tagatcgaag aaaagagatc tacaattttt atgaagtcga agcaagccta
    10921 cgatatggca tcggaaaatt gtggttagat ggaagtaata ttcgtcaaaa caaaccatac
    10981 attttttgga acagtagtga tttagaactt ccaatgaaag ataaataatt atggagaatc
    11041 gtttgaaaat gattcctcta tacaggattg tgcttatagt tggcatggag ttaaaaacct
    11101 taaagaagga gcagttattc aaagtgaaag aacgtcataa tcagttacta tgtagtaaaa
    11161 cgggtacgat gaacattgaa taccgaacag aatatttaga attaacgaat caaactgatt
    11221 ttttgttgat tcgaaagcaa caagagcaag aaaaaaatta atggctactt atagccaatt
    11281 gattgatgaa tgggaacaat tcaaggaatc taaaattcca gaacgtctct aaaagccttt
    11341 atttgtaaag agcataagga aacgtgggaa cactttttaa aaaaagtgtt ccaataggct
    11401 gtgcctatta cgtttctcaa aaataaatgt gggaacaaaa tatttaaaaa taggatagtg
    11461 aaaggatagt gaaggatagt aaaaggaggg tagtaggata gcaaaaagga taggctcctt
    11521 ttcgctatcc tttttaaatt ggaggaatta atatggaagt aagaataaga aatgtggatg
    11581 agaaaatcgt aagagaaatt gatcgaagat gtgacgaa
//
LOCUS       DACJGB010000086        11313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.088, whole genome shotgun sequence.
ACCESSION   DACJGB010000086 DACJGB010000000
VERSION     DACJGB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.088"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            544..1107
                     /locus_tag="IYI34_002538"
     CDS             544..1107
                     /locus_tag="IYI34_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560370.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="IYI34_002539"
     CDS             complement(1386..1673)
                     /locus_tag="IYI34_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR0560371.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="IYI34_002540"
     CDS             complement(1663..1992)
                     /locus_tag="IYI34_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR0560372.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="IYI34_002541"
     CDS             2297..2626
                     /locus_tag="IYI34_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560373.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYI34_002542"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="IYI34_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAR0560374.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="IYI34_002543"
     CDS             complement(3551..4462)
                     /gene="vanY"
                     /locus_tag="IYI34_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAR0560375.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="IYI34_002544"
     CDS             complement(4890..5498)
                     /locus_tag="IYI34_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAR0560376.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYI34_002545"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="IYI34_002545"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAR0560377.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="IYI34_002546"
     CDS             complement(6528..7496)
                     /locus_tag="IYI34_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAR0560378.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            7828..9120
                     /locus_tag="IYI34_002547"
     CDS             7828..9120
                     /locus_tag="IYI34_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR0560379.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(9218..10372)
                     /locus_tag="IYI34_002548"
     CDS             complement(9218..10372)
                     /locus_tag="IYI34_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAR0560380.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(10350..11045)
                     /gene="vanR"
                     /locus_tag="IYI34_002549"
     CDS             complement(10350..11045)
                     /gene="vanR"
                     /locus_tag="IYI34_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAR0560381.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
BASE COUNT         3397 a         2288 c         1946 g         3682 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ataggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tagttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatta tggctctata ctttttgatg ttggtggatg acaccagcat
     7741 cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg
     7801 cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta
     7861 gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa
     7921 gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac
     7981 tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta
     8041 atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc
     8101 aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc
     8161 agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag
     8221 atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat
     8281 cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa
     8341 tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc
     8401 ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt
     8461 tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta
     8521 tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc
     8581 caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa
     8641 agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt
     8701 caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct
     8761 catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta
     8821 ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt
     8881 gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg
     8941 ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca
     9001 cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc
     9061 ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa
     9121 aaaagatcaa gaggaactga tccacttgat ctccaattat tctaagattt ctctttacca
     9181 acatcatttg acaaagagcc aaaattatat acatctctta ggacctcctt ttatcaacca
     9241 agtctggcat cgctggaagc tctaccctaa acgtcgtata gttatcattg ctttccgcgt
     9301 aaatctgccc tccatgctga acaataattt cttttgcaat cgccaatcca agtcccgcgc
     9361 cacccgtatc ggaagaacga gcattgtcca gcctatagaa cttttcaaat atggcagcta
     9421 gcttatcttt tgggatgctt ccagtgttct tgaattcgat tgacaccaca tccccggaga
     9481 ggcccgcggt aatgtcaatg atgctgttat cctcactgta tgcagcggcg tttttcaaaa
     9541 tgttgttaaa gactctcgcg agtttatcag ggtcgccgga cacggtcaga tcctcggggg
     9601 cgtgaataac cgcctgtttt ccatgtgcgg aaagctgagg ataaaattca tcggtcatct
     9661 gcaccagcat atagtatagg tctatgtgcg tttttgttag cgttatcgtt tgtaggttat
     9721 accgtgtaat ctcaaaaaac tcgtcgatta gctgttcgag tcgatacgct ttgtccaacg
     9781 tgatatgcac atactttgcc ttttgatcta ccggcatgtc tggagcctcg tcaagcaggc
     9841 tcaaataacc gataatggat gtaaggggcg ttttaatatc gtgcgccaag tacataacaa
     9901 cgtcattttt tctttgttcg gccagctttg catcctgctc tcgcttttcc agagtccgtt
     9961 ttaatgtgtt gagcttttgt tccataacat ccatttccgc agaaagctca atttgtttat
    10021 cttcgttctg aataagtaca tcaatgccgg tatttatctc gtcaaagtat tttgcgaatt
    10081 ttgaaagcat gacgcgacat agaataagaa tactaatgac aatcgccaca taaataaaga
    10141 tatctatatt gttccgtatg gaatattgat ataatttcat cgcgtccagg tgatttaagt
    10201 catatttgtt ttccaaaata cttaagatcc aatccccaag tttccctcgg atcattgaac
    10261 gaatatacaa cacgaataca attgctacca caacaattgc aacgatatac atgtaaagtt
    10321 ttcgttctag tttggaatag tcgttttttt tatttttcaa ttttataacc aaccccccat
    10381 accgttttta tatatttcgg attatcaatg gtgtcgttca ttttttcgcg caaatgccgg
    10441 atatgcacgg tgatggtgtt gttgctcttg ctgaaatatt cgtcgcccca tatctcatga
    10501 aatagcagct cggagctaac cacattcccc ttgttttcac agaggattcg cagtattgaa
    10561 aactcggtgg gagtaaggga taactgcttc tcgttcagat aacactcatg ggtgttaaca
    10621 ttaatgacaa ggccggagtg gacgataaca ttttcgttct gctcctttac tccactgaat
    10681 tttttgtatc ggcgcaactg ggcctttacc cgagcaatta actccagtgg gcgaaagggc
    10741 ttcgttatat aatcatccgc gccgattgtt aaccctgtaa ttttatctac ctctgtatct
    10801 ttcccggtca gcatgataat cggataggtg tgcttgtccc ttattttttg acagatagta
    10861 aggccgcttg tgccgggaag catgatgtcc aatatggcaa ggtcaatctc agacttgtct
    10921 atacattcca atgcttcttt ggcggtatag tatttgaaaa ccgtataatt ctcgtttttt
    10981 aagtataatt caaccaaatc ggcaatttca tgttcatcat ccacaataag tattttatcg
    11041 ctcatagtta tcaccctttc acataagttt gcccttaata atttatcaga ttatagggcc
    11101 gaatttataa gcatttacat aataaatgac taagaatttc ttaagatgat gattcttttt
    11161 ttatcaaagg gcttttcggc atcgctagga ggctgttaat attttatagt ttccttttct
    11221 tctttaaaaa tattgcccat tagcggaata cagaatggct aattattcac ttctgataat
    11281 ttcctgtata atgaagccct agatacatta gta
//
LOCUS       DACJGB010000087        10660 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.089, whole genome shotgun sequence.
ACCESSION   DACJGB010000087 DACJGB010000000
VERSION     DACJGB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.089"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..537
                     /locus_tag="IYI34_002550"
     CDS             <1..537
                     /locus_tag="IYI34_002550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560382.1"
                     /translation="NMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNG
                     QPMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLD
                     MTIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQA
                     IIDSGRVTQWKKIQPFIF"
     gene            564..2375
                     /locus_tag="IYI34_002551"
     CDS             564..2375
                     /locus_tag="IYI34_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAR0560383.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEARPTDSMKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKR
                     IESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIH
                     ELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIAAWTKNGQTLDDK
                     EAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTLETPENKNI
                     VPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSE
                     EFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2436..3896
                     /locus_tag="IYI34_002552"
     CDS             2436..3896
                     /locus_tag="IYI34_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAR0560384.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3907..6111
                     /gene="topB"
                     /locus_tag="IYI34_002553"
     CDS             3907..6111
                     /gene="topB"
                     /locus_tag="IYI34_002553"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAR0560385.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6351..6944
                     /locus_tag="IYI34_002554"
     CDS             6351..6944
                     /locus_tag="IYI34_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAR0560386.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6957..7856
                     /locus_tag="IYI34_002555"
     CDS             6957..7856
                     /locus_tag="IYI34_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAR0560387.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7859..8356
                     /gene="ssb"
                     /locus_tag="IYI34_002556"
     CDS             7859..8356
                     /gene="ssb"
                     /locus_tag="IYI34_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAR0560388.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8722..8982
                     /locus_tag="IYI34_002557"
     CDS             8722..8982
                     /locus_tag="IYI34_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560389.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9427..9633
                     /locus_tag="IYI34_002558"
     CDS             9427..9633
                     /locus_tag="IYI34_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAR0560390.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9681..9884
                     /locus_tag="IYI34_002559"
     CDS             9681..9884
                     /locus_tag="IYI34_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560391.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9900..10337
                     /locus_tag="IYI34_002560"
     CDS             9900..10337
                     /locus_tag="IYI34_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560392.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10330..>10660
                     /locus_tag="IYI34_002561"
     CDS             10330..>10660
                     /locus_tag="IYI34_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560393.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVS"
BASE COUNT         4025 a         1784 c         2234 g         2617 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc aaccatttat tttttgaaag
      541 gctatcgaaa agttgcttta gaactgaatg aaaacggggg atttgaggaa tcaaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctagac
      661 caactgattc tatgaaggag aaagaaaacg atcatttcta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacattggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaggaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaaccaatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatacccat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaagggtt ttatgagaaa ggacgtggct ttgttcgaga aacagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaccttac tatacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc ctacgcagca tttagctaca agtattcatg
     1741 aattagccca tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc
     1801 aggcagaaat gacttcttat attgtgtgca aacattttgg aatggacacg tcggagaaag
     1861 ctattcctta tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg
     1921 cggaacgtgg gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca
     1981 tttctaatga aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga
     2041 agcaggagca taaaaatact ttagaaacgc cagagaataa gaatattgtg cccgttggtt
     2101 cactttggat cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac
     2161 atttgaagga tgcggcgttt attgaagatc cgcagcataa tcaagtaatt ttaaaaggaa
     2221 aaagttatgg aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatc
     2281 cattccgatt ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt
     2341 atgaacaaca attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa
     2401 aacggatagt cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta
     2461 ttgatcgatg taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag
     2521 tcaataaagg acgagattat cgcttagaag accatgattc atttgttttt gatcgaagaa
     2581 aacaacgttt ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac
     2641 tttttttcat agacaaagag atccaagacc ctaagcaaca atttaaagca gccactgatt
     2701 ttattctaaa gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gaaacagaaa
     2761 agtacaaaga tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga
     2821 aggaagaacg aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg
     2881 ccgaactaaa gccaaaatat gaacggcaaa atttcttagt tcgagacgat cgtttagatc
     2941 atgcggtcgc ctttttatgg aaagatccac agacaaaaga gacagtaggg gcgagttatc
     3001 aaggaacgtt cattgattat gaacgttttg gtgagagagg cacatataag catatagata
     3061 aaaattcgac agcgaatcat ggctttaacc taaagatcgg tgaccctaag cagataaaat
     3121 tttttgagag tagtattgat ttacttagct atgctgcact gaatcgtgat cagttaaacg
     3181 atacgtggtt agtctctatg gaagggttaa aacaccatgt gatcagtcat tattttggtg
     3241 aggcagtatc tgaattgaga aagaaacaag cctttcctca atccatcgag atttgtgtgg
     3301 ataatgaccg tgcaggacat attttctacg aaaaagaaca attaatgggc gcagtcgatc
     3361 catttacgaa tcagaaagta cgttgtgaac gaggaatcgc taatgattgg caagtgccaa
     3421 aagaatacaa aatcatttat gaagaagtcg caaaagaaga aaaggtcaca ccagaagcta
     3481 ttatggccat acacaagaca gaaaacaatc tgcaattaac gaaccaactg gtttccgctc
     3541 acaaagtaaa tgcgtttttt ggccagcagc tttctgttaa cgattcaata gaagccatta
     3601 atttgaaaga tatttgtaga gaagtcgcaa aagaattaaa agtttgtgaa cgcgtggatg
     3661 gtacgtacga ttttgatcgt ttttatcaag aaaaagggga tattaatgcg cagattcttt
     3721 tctcttataa agcagaacaa tactacaaag gctataaaaa tcatgaacat gaatttgtac
     3781 ctgaagtaaa aaaggactgg aacgatcaat tgaagcatga gatccagcaa caagaaatca
     3841 gaaagcaaaa acgtgccatg ttgtttcaac aaggcagaca acaagaaagg gagtgaaatc
     3901 gtcatgatga aaaccgttat tttagcagaa aagccgtcac aagcgaaagc ttatgccgat
     3961 agcttctcta aagccactcg aaaagacggg tattttgaaa tccaagatcg attgtttcct
     4021 ggtgaaactg tgatcacgta tggttttggt catttagtcg aattagattc gcccgatatg
     4081 tacgatgaaa attggaaaca atggtcatta gaacatttgc ctattttccc tacacattat
     4141 cactatcatg ttcctaaaga taagaaaaag caattcaagg tggtgaaaca gcaacttcaa
     4201 tcggccgaca ccattattat tgcgacagat agcgatcgtg aaggggaact gatcgcttgg
     4261 acgatcattc aacaagtagg cgccgatcaa ggaaaaacct ttaaaagact ttggatcaac
     4321 tccttagaaa aagaagccat ttatcaggga ttccagcagt tacgagacgc aaaagagacc
     4381 tatccaaaat ttgaagaagc acaagcacgt cagatcgccg attggttaat tgggatgaac
     4441 ggtagcccac tgtatagttt actcttgcag caaaaaggca taccaggaag cttttcctta
     4501 ggcagagtcc aaaccccaac gctttacatg atttatcaat tgcaggaaaa gattagaaat
     4561 ttcaagaaag aaccttattt tgaaggaaaa tccgaagtaa tcgctcaaaa tggcgcattt
     4621 gatgcgaagc ttgatccgaa tgaaacccaa gcgacgcaag aagcatttga agattattta
     4681 aaagaaaagg gggttcaatt gggaaaacag ccaggcatga ttcgccaagt tgaaaccgag
     4741 aaaaaaagcg cggcaagtcc acgtcttttt tctctatcca gccttcagtc taaaatgaat
     4801 cagctcatga aagctagtgc caaagacact ttggaagcca tgcaaggctt gtatgaaggg
     4861 aaatatctaa gctatccgcg aacggatacc ccttacatta cggaagggga atatgcgtat
     4921 ctgttggatc acttagacga atacaagcat tttttgaggg ctgaagaaat ttcgactcct
     4981 attcatacac cgaatagtag gtacgtgaac aacaagaaag tccaggaaca ttacgcaatc
     5041 attcctacaa aaacagttat gacagaagcg acgtatgaaa gtctgacacc tttacaacaa
     5101 gcgatttacg agcaagtttt aaaaacgaca gtggcgatgt ttgcggaaaa atatacctat
     5161 gaagaaacaa cgatcttgac gcaagtccag cagcttcagt taaaggcaat cggaaaagta
     5221 ccgatagatc taggttggaa gaagttattt ggcaaagaga gcgacggaaa agagaaggaa
     5281 gaagagccgc tacttcctaa agtgacaaaa ggagaaatgg tgacggtaga tctacaagtc
     5341 cttgaaaaag aaaccaagcc accacaacct tataccgaag gtacgttgat cactgcgatg
     5401 aaaacagctg ggaaaaccgt ggatagtgaa gaagcgcaat ccatattgaa agaagtggaa
     5461 ggaatcggta cagaagcgac acgggcgaat attattgaaa ccttgaaaca aaaggaatat
     5521 atcaaggtag agaaaaataa gcttgtggtg acgaataaag gggttttgct ttgccaagct
     5581 gtggaaaaag agccgctgct tacgagtgcc gaaatgaccg ccaaatggga aagctacttg
     5641 ttaaagattg gagaacgaaa aggaacacaa accacttttc tagccaatat ccaaaagttt
     5701 gtctctcact tattggaagt cgtcccagga caaatccaat ccattgattt tggatcgacg
     5761 ttacaagaag tgaaagcagc cagtgaaaag caagaagccg cgcgtcattt aggaatttgt
     5821 ccaaaatgcc aggaacaaga agtcttactc tatcaaaagg tggcagcttg tacgtctgaa
     5881 gcctgtgatt ttaaattatg gacaacgatt gccaaaaaga aactcacagc gacacagctg
     5941 aaggagatta ttcaaaatag ccgaacaagc caaccagtga aagggttaaa aggtcagaag
     6001 ggttcgtttg aagcaacgat tgttttaaaa gaagacttca ctacaggctt tgaattttct
     6061 gaaaagaaaa aaacgaactt caagaaaaga acacgacgca cgacaaaata atggagggtt
     6121 aatcttatgc gtaatacaac acacaaacaa caagaaatcg aaaatcaaaa agccaaaatt
     6181 cgttcagaac aagagaaatt acgtgccagt aaaagaagac ttcagcaatt aaagaaccag
     6241 caacggattc aaaaagaaat cgaattagat cgatagacat taaccttgtt acaaatacac
     6301 tatcatgata tatttaatgt agtgtatttg taacaaaagg ggggaagtaa atgcaagaga
     6361 aactgaaaga attgttggct attcgcgatg gaaatttatc tatgaaggaa gccagaaaat
     6421 taggaatcgc agcaaccact gtacaaagat tggtgaatca aggaaaattg atcaaagttg
     6481 atcgtggtta ttatgtagag gatggtcatg gaatagatga tttattttgg cttcagcaac
     6541 gtttttccag gggaatcttc tctcatgaaa cgactttaga tatttaccag ctttctacga
     6601 atatgccgaa attcattcac ttaacttttc cccatggata taacgttgat cgttcaatta
     6661 caaaagagca gcagttacaa tttcatttcg tgaagaaaga agtttatgaa ctaggaaaag
     6721 tagagggaac ttcctttcaa ggaaatccta ttactatgta tgataaagag cgtacacttt
     6781 gtgatatttg gaatccgaga tatgaaattg aatatgaaat gaaattagaa gcattaaaag
     6841 agtacatgga agacccattg agagatccaa gtaaattaag agattacatg acaaaactac
     6901 atgtagaaaa agaaatgaaa taccacatgc aaagcttata ttgatggagg aaaaagatga
     6961 caccgacaca atttgcagca aaaacaagaa atttagcaaa gaggacagga ttaaatgcac
     7021 agttagtaca acggcatttt atgatggatc gtttaattga acaaatttct gaatctaagt
     7081 ataaaaatga tttcattttg aaaggtggct ttttattagg atcaaaatat ggaatcgata
     7141 gaaggacgac aattgatatc gacacgacat ttagaaatag caaattaact gaagaaaaat
     7201 tgaagagtat ttttaatgat ttaactagga aacccacgaa agaaggtatt gttttctcta
     7261 ttcaaggaat gaaggaaaca agagaagccg atttttatcc tggatttcaa attaaattgg
     7321 tggctaattt agagaaagta cgtgtgccat ttaaaatgga cgtgacaaca ggagattcta
     7381 tttatcctga agtagataca cactatcacc aattaatgtt tgaagataaa aaggtggaga
     7441 ttccagctta tccaacagca caaattattg cagaaaagct aagtgctacg ttcagctttg
     7501 gcacagataa ttcgagagca aaagattact acgacttatt taccattccg aaactagaaa
     7561 atatcgatac gaaagagtta tacaaaagtg ttcgaaatac atttacaaaa cgtggggata
     7621 caaaaccgtt gtccttttat tatgaaagag aaatggataa gatcatagga aatgactatt
     7681 tggaaaagca gtgggaaaaa tatcgctcag ttaacacgtt tgcgaattcc atttcattta
     7741 cagatacagt caacgaaatt gatcacctaa tgaaaagtgt gatcaaaatt gaaaatcaag
     7801 aaagaaaacg gcagtttaaa agatcacaac aaatgaatca agaaatggag agataaagat
     7861 gatcaataat gtaactttag taggacgctt aaccaaagat ccagatttac gttataccgc
     7921 gagtggtact gcagtagcga cttttaccct agctgtcaac cgaaatttca cgaatcaaaa
     7981 cggcaatcga gaagctgatt ttattaactg tgtgatttgg cgcaaaccag cagaaacaat
     8041 ggtgacatta gctaaaaaag gtagcttgat tggtgtagtt gggcggattc aaacgcggac
     8101 ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtggccg ataatttcca
     8161 actattagaa agcaaagcag cgacagaaag ccgtgcgcac gctgatcaat cgtccaccag
     8221 tccaagcacc accacttttg aacaacggga tacagcgacg cctaataaca atggattgaa
     8281 cgctttacag aatccatttg ggaatccatt tggtggacaa tcaatcgata ttagcgatga
     8341 tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt tatgttataa
     8401 tttagtcaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg cgggctagtg
     8461 gttttctttt acgtttctat tataagaatt aacgggtagg cttactgtct ttcttggcaa
     8521 atttacaatc aagccatttg cagatatagt gcgcggctac ttctgccatg attgcaaaaa
     8581 caaaatcaaa aagaattttc aaacctataa cctcctcccc ttgccagatt aggccggggt
     8641 agcagacagc aagactatta tagcataatt gaaatatcca aatgattgga tttttgctat
     8701 aattttagcg aggtgattgt gatgaacaaa tatgttggtt tgcttgataa aatccgagta
     8761 ataaaaacgc aaccgttatt ggttcgcttt actttacaaa cgattcatga atcgattaat
     8821 tgtgtagttg cagatattga aattatcgat aaattgctaa ttatggatga tggaaaatac
     8881 aatattgccg tcacaggtca cttcaataaa cgaaatcagc tagttattgc tagcatgtat
     8941 gttagaaatc ccgatcattt tacgcgatcg atgggcatat aaaaaaagaa gagttgcaat
     9001 cctaatgtag gactgcagct ctttttgttt tacatagaaa caagaaaaat gaaaaggcct
     9061 ttttaggaag gcgcgagcgc cttctcaatt gaaaaacctc tctcactcca accgccaccg
     9121 ccgttcgaga agaaaggaag tgaacgttgc acgttcacta agataaaggg tcttgcaaag
     9181 caagacaaaa tagaaaaaac ttcctctcac caaaaggttc gttcctgttc gttatctctt
     9241 tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag
     9301 ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa
     9361 caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa
     9421 tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac
     9481 tatcatgcac cacatactgt cgaatgcgat tgtggacaaa gagcgaaatg tatcaaaagc
     9541 tatcgtttgt atcaatgtcc aacatgcgga aaaaaataca ccttgtctta tggcgattat
     9601 attttgattg atgagaaagg aaagaaacgc tgatgaaaac ataacataaa ttattgactg
     9661 taaaagaagt cgttcaaatt ttggtggaca aagaagtcat ttctgaaaaa gtagcagaag
     9721 ctttacttat tccgcggtac tggacgatag aaaccccttt agtagaggat gcaccgacta
     9781 tgctatttga agacctagtg aagattgcag gatatgaact gcattatcaa gaaaacatgg
     9841 aaacttatga aaacgaatat tggttaacca aattagagaa ctaaaaagga gaaaaaaata
     9901 tggctcaaaa ttactatgac gaatttgtca aattaccttt ggataaaatg gcacaaaaaa
     9961 tggaggatat gacattccta taccatgaga cacgtgttcc aaaaaaacat tataaggaaa
    10021 gattgtcggt agctgtggaa gaaatgattg aatcaggtgt cgaaatgaat ctcattgcga
    10081 cgtactatcg aacactggaa gaactgaaaa aacaaaatgc aaaatggttt ttccaagcct
    10141 tactatgctt ggaagtaggt gtcaaaccaa gcactattaa accttctgaa tatcaagctt
    10201 tagaactaac gtatgggaaa tttgtcgaaa cgaaaaaagc caagactgtt tcaagtgaat
    10261 ggttagatta ttttgaaaat atcaataagt atggcgccta ttacaccatg aaaaaggagg
    10321 acaacgaaaa tgaataaggt gatttatcaa ctaacaacgg aagatgtgtc acacttttta
    10381 acaaacaaag aacatttgaa tgaagtaatc agacggattc aaaaagactt tagttgtatg
    10441 gaagaaatga agtactttat tcaaagagtc gaagaacaat tgtatttgga aagtgaaacg
    10501 agtcaaacga aggtcacagt cagcaataat tatttagacg tgtataaaca tcgtcctgaa
    10561 acggtaaaaa aaggaacgga gcttctacga ctagaaacag aaaaatacat catgaatgtt
    10621 tacctagaaa ggagtgaact ggtcgaaaac tttgtcagct
//
LOCUS       DACJGB010000088        10357 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.090, whole genome shotgun sequence.
ACCESSION   DACJGB010000088 DACJGB010000000
VERSION     DACJGB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.090"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            172..516
                     /locus_tag="IYI34_002562"
     CDS             172..516
                     /locus_tag="IYI34_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAR0560394.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            517..936
                     /locus_tag="IYI34_002563"
     CDS             517..936
                     /locus_tag="IYI34_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAR0560395.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1028..1378)
                     /locus_tag="IYI34_002564"
     CDS             complement(1028..1378)
                     /locus_tag="IYI34_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560396.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1344..2675)
                     /locus_tag="IYI34_002565"
     CDS             complement(1344..2675)
                     /locus_tag="IYI34_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAR0560397.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2931..3497
                     /locus_tag="IYI34_002566"
     CDS             2931..3497
                     /locus_tag="IYI34_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAR0560398.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3642..3729
                     /locus_tag="IYI34_002567"
     tRNA            3642..3729
                     /locus_tag="IYI34_002567"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3674..3676,aa:Ser,seq:cga)
     gene            complement(3781..4986)
                     /locus_tag="IYI34_002568"
     CDS             complement(3781..4986)
                     /locus_tag="IYI34_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0560399.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5124..6012)
                     /locus_tag="IYI34_002569"
                     /pseudo
     CDS             complement(5124..6012)
                     /locus_tag="IYI34_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(6099..6299)
                     /locus_tag="IYI34_002570"
     CDS             complement(6099..6299)
                     /locus_tag="IYI34_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560400.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6328..6750)
                     /locus_tag="IYI34_002571"
     CDS             complement(6328..6750)
                     /locus_tag="IYI34_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAR0560401.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(6767..7189)
                     /locus_tag="IYI34_002572"
     CDS             complement(6767..7189)
                     /locus_tag="IYI34_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560402.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7480..7671
                     /locus_tag="IYI34_002573"
     CDS             7480..7671
                     /locus_tag="IYI34_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560403.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            7682..7948
                     /locus_tag="IYI34_002574"
     CDS             7682..7948
                     /locus_tag="IYI34_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560404.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            complement(8189..8668)
                     /locus_tag="IYI34_002575"
     CDS             complement(8189..8668)
                     /locus_tag="IYI34_002575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560405.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            8713..9420
                     /locus_tag="IYI34_002576"
     CDS             8713..9420
                     /locus_tag="IYI34_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAR0560406.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            9599..9940
                     /locus_tag="IYI34_002577"
     CDS             9599..9940
                     /locus_tag="IYI34_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560407.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            9933..>10357
                     /locus_tag="IYI34_002578"
     CDS             9933..>10357
                     /locus_tag="IYI34_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAR0560408.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFD"
BASE COUNT         3497 a         1702 c         1905 g         3253 t
ORIGIN      
        1 ataaaaaaag cgttagcaac tatgcttaaa aagcatatcg actgaataaa ctaagcattg
       61 gacaggctga aagactacat ttaaaatgaa atgcgaagaa acaatagaaa ataaagttgc
      121 cttagaacca tagttgaatc acctaacagt cgtgaaggga gaataagaga aatggcagaa
      181 aaacaaaccg caggacgaga tcaattagga gattttgcac caaaatttgc agaattgaat
      241 gatgatgttt tatttggaca agtatggtcc agagaagaat tatctgctca tcagcgtagt
      301 atcatcacca tatctgcatt aatttcagga ggaaattttg aacaattgcc agctcatctg
      361 aagattggca agcaaaatgg cattactagt gaagaaatag cagaaatcat tacccattta
      421 gctttttatg taggatggcc aaaagcttgg tcagctttta gtttagcaaa agaaatctat
      481 ttagaagaaa atagcgaaga taagaaagag gcgtaaatga cgacaaaaca cgaagaaata
      541 cacaatggcg tgatttttcc agcaggtgaa aaaaacgaag cttacgctca agtatttacc
      601 gggcaaagct atctgaatat gttagtgaat gatcccgaag tttctgttgg tgtaggtaat
      661 gtaatttttg aaccaggttg tcgcaatcac tggcatgtcc ataaagacgg ctatcaaatc
      721 ttacttgtga ccggaggaga aggctggtat caggaagaag gaaagccagc aagacgatta
      781 atggctggag atgtcgtcgt aacgcacaaa gggattaaac attggcatgg tgcgacaaaa
      841 gacagttggt ttgaacattt ggcgatcact gctggtaatt cagagttttt agaacctgta
      901 acagatgaag tgtatgaagg attggaaaat caataatgtc aagcttgaac agctcctgaa
      961 agtagatttt aatcgaactt tcaggagctg cttttttgat tcagtctttt ataaatagaa
     1021 aatagggtta ataataggaa tgagaagcac gtcgaaagat cttaatattg ataatcggct
     1081 gggcatacat gatttccacg tcatcaatca aactacctaa cagatgataa tcttcccgat
     1141 catgtcgggg attacctaat agttcttgat agtagatttc catactaggg tcccgaggat
     1201 tgttcagcaa atcagcaagt acatctaaat cttctggctg agtttctgcc gtaccctgta
     1261 tctctatgaa aagcccttcc tcagcaaatt ctactccaat acgtatttca gttatttcat
     1321 gggaatataa ataagtaaga agctcatcga tgattctttt tgataattct ttttccaagt
     1381 gtcttttttt cctttctgac agcggtaaag ttatctagta tcggtacaag gatacctgca
     1441 acgaaaccag ttgaaaaacc actgttatat agatttagtc ctccgtgcaa agtgctgacg
     1501 ttatggacaa gagcaatatg tagtacacca gctattacac cgtaaaaact gccgtaaaaa
     1561 ccgcttatgg gcgcgagacc tgtcccaaac acagctgcaa caagcatgcc agtactggtg
     1621 atctcatgaa aagttccaaa caaagaagca ataaatattc cgactagtat cggaaaactg
     1681 ttttttaaat gacaaccaaa agcactgaaa cccaccgcag ataaaatagc accgatgaca
     1741 ggtccattta actgaccgcc aaccagcaaa acataagaag acagcatcag accaactaag
     1801 cctatgttga tcaaagtagc accaatacca gaattagcaa tgaaatcagt tgtcaatttt
     1861 ccagatgaat cgaaaatttc tcgaatcccc aaaagccgaa aggaattata atagaaacca
     1921 attccgactg tcagcgaaaa aaagccaaaa ataatccaaa tgagaggaaa gtgatattca
     1981 ttgaaaacca atgtattttc gacgattgaa tagctgaata acctcaaaac agcagtaaaa
     2041 gtcatcgcaa tcaacccaga ggtgaatcca atattataaa gtgtgaatcc acgatggaaa
     2101 actaaagttt gagcagctaa gggcggaaga agtagaccaa ttgctatgcc agctaaattc
     2161 cccaataaaa tacctatcag taaaggaaaa cgcataccaa aagtgatata actaacgacc
     2221 ggtgataatg cgctaccaaa cagtccgatc atgatgtact gagaaaaagg tttattgaca
     2281 aatacagcgt atagatacac acctaaaatg attgagatag aattataaag gtttttgcca
     2341 aaaaaagaga aaccggtaac tgttagaatc gaagcaaata agggaccgtt taaaatcacc
     2401 ttgtgccgat aagctaaaag cacagaaagt aaggtcaaaa tccctacatt tatgaatgcg
     2461 cttccgaaac cgcctaaagc aaaataatct gtcaaaagat tgcttggcga ggtaagaata
     2521 gttagcaatc cactccaatt atcagtagga gtattagaaa gcaagccaat aaaaagaaaa
     2581 aaaacacctg taaaaccaag ataaaaatac tttcttttct gtgaaacgat tcgtttttga
     2641 ggatgaatat aaataacgtc agtttcgttt tccattacac accttctttt tcattttctt
     2701 taactataac ttgaaatgaa agatttggac aactatcatt taggtcaaaa ctaaaaatgt
     2761 aagggaaccc attgaattta accaaatttt tttaattttg caaaaaaaag ttaaagaaga
     2821 ctgaaaaaaa atttaaaata taacgttttg agtaaaatgt ttgcaaaaaa tgctaaaaat
     2881 agggtacaat aaaacaacat atactgcagt aaagaagtgg gggagtttta atgagaagaa
     2941 aagtctatac caaggatcaa attctaaaag ctgcttatga tgttgttgca aaggaagggt
     3001 ttaaaggatt tactgcgaga aatattgcta aaaagatggg gatttcgaca cagccgattt
     3061 atctggaatt caaaaacatg gaagatttaa aaaatacgtt gctggattct atctttgaac
     3121 aactaaaaga agatgttttc cctattgaac gtactggtga taaaatcatt gatctcggtt
     3181 tgaactatat ctactttgca aaaaagcatc atagtcttta catggcattg tacattgatg
     3241 aacatgggga cggaaagaaa atgcatgatt tttcatacaa ttatttccgt gaaatagtta
     3301 aagaagatga aagatacaat caattatcgg atgaatatgt cgatgctctg catactggta
     3361 catgggtagc agtgactggc ttagcgtcat tgatgtcatc aaacatcatg catccaacag
     3421 aagaacaaat cattgcactg atcaaacgga caattgactc gattttagca ttagataatc
     3481 ctgaaataat cagatagtgt tctatcagta tgaatttgat cggtaagtcc gttggctgca
     3541 aactgtcaat ggactttttt gctaaaaaaa gcgatttctg ttatttatct attagaaata
     3601 ggcttgacat ttcgtgaaaa aagtttaaaa atagtactta cggaaggttg gcagagtggt
     3661 aatgcaccgg actcgaaatc cggcgaaccg gctaataccg gcgcgcaggt tcaaatcctg
     3721 taccttcctt atatatcaac gattagacac gatttcgatc gtgtcttttt tgttgtatat
     3781 ttagcctaaa atgttttttg actccccttt tgactccccc tgagtactgt tcttcataaa
     3841 tgctacaaaa ttattgatga cttttttgtt ttgagtttca gttacatgag tgtaaatatt
     3901 tgaagtagtt tgaatgtctg catgccctaa acgttcctga acatctttta aagaagctcc
     3961 tgattcaaaa aggagagagg catgagtatg tcgaaaacca tgagtagtga ttcttttagt
     4021 caattctgga tgatgcttat atatgacatt aagccacgaa cgtgaaactg tagggttata
     4081 gagaagacca tcatggtgaa aaattaagtt ttccctttgg attgtaacga tcgttttgtt
     4141 ttttgtataa tactttctca ataaactgat caaatcattg tccagataaa tttttctttt
     4201 tccagatttt gatttaggag tatttacgat caatcttcct tgataacctc ttgttacagt
     4261 tttattgata ttgagtgttt tgctcttaaa gtcaatatcc gaccaggtca gagcaaaagc
     4321 ttctcctttt cttatacctg taaaagccaa tagggaaaag aataaatatc tttcatcatc
     4381 atcgtttctt attgcttcta aaaatttgat taattcttct ttagtataaa attcgatatt
     4441 tttatcttcg atatctagag cttctccacg tggaatagaa actaatttca taggattgct
     4501 agttataatt tgtaaagaag cagcgtaatc aaaaacatta gatgtgtaat tttttatttt
     4561 cttaaaaatt ttaggatggc tatcggacca agtgtttact gcttcttgac agaaaaaaac
     4621 gtctattttg tcaatgaact tatctccaaa cacttttaag atgtgtgtgt caaaaatttg
     4681 tttggtggag gaccaagtgc tttctttaac tgtcttttta taattttcaa accataagcc
     4741 gtaaacatat tcgaatttct tgctcttttg agctgtagaa gcttgcagtc ctttttcctg
     4801 tatatctgcc tccaatcttt ttaaagctct ctcagcggct aatggcgtac taaaacctcg
     4861 tctcgttgtt tttctttttt ttcctgttag aggatcaact cctaagtaca gactaaactg
     4921 atatttttct tcgccttttt ttgttaagtg ctttttaatt cgtttatcaa tttctttttt
     4981 tgccatcttt atctttcctt tcgtacgttt gttcggttgt acagcggatt tcgagatggt
     5041 aaaatagggt acaacaaata gacctacttt accgtagctc tttgcacatt tgcgttcttg
     5101 gtcgggcggc aaatgtgctt tttttatttc actcttagtt cttgtcctgg ataaagcatg
     5161 tagttgtttg gatccatacc gtttaatgca aacagttgat ctaccgtaat tccagctcgt
     5221 tcagctattt gtttggtccc ttcaccaggt tgcaaagtca aggtttcggt ttcagatgaa
     5281 gaaggtgccg ttggttgagc ttgattagct ggtacataat cttgtggtac tgattgctcc
     5341 gttgaactgg gagtgggtga aacttgttca acacttgaac taggagtagc ttctgcatag
     5401 ttttctacac tttcagcaga gtaagtacta ctagtttcag gaggcgtatt ctgcgcaata
     5461 gcagcttgtt ctacagtact agaagatgca atagtacttt ctgttgtagt cgaagatgat
     5521 tgttcgttgc tacttgttga gctagtagtt gttctagatt ctttagtttt tttccttttt
     5581 gtagggtttc aaaactaatt tctcattatc atcagatttg ttaggctttt ctggagttaa
     5641 aataatattt ttatcttctt tgctgacagt atatttggca tcattatctt tgtctttatc
     5701 atctttccaa gtcataacat cgccttcaag tgtatactca aatttgtaat tcatttggtc
     5761 gactaagctt ttagcaaatt cttctcccat agcctcccaa tcgttacttg aagttgattt
     5821 catagaactt gtgtcaacac taaaagatac gacgtgatca gaaaatgata cgatcatatt
     5881 aggttcgtca tctttggttg cttcaatcaa ccaatcatta gcttttaaat cttctgtagt
     5941 tactttttta ccacaagcag tgaaaagtaa cagagaagca aaaactaata aacttgttat
     6001 tacttttttc attttttatt cctcatttct atgatatgat ttttattggg gaatcttaga
     6061 aataaggttt cgagtccgtg ttgctgcacg ggcttttttt atttaaataa atcccaaaaa
     6121 ctgaatgtag ttttcttgta tactttattg taagcagcct tttttgggtc tttaatccag
     6181 ccgctacctt ttttcccata accaggaata actgcttttt ttacttttct ttttgctttt
     6241 ccagtggttc tagcgcttat ggattttttt atacttggtt ttctcattcc tattttcatc
     6301 tatatcctcc ttacgttgta ttataattca caagaaacga tcgaattcat ctggtaatcc
     6361 aaagcagacg agcttttcat attttgtcat atgatcaaag ccgtctaaag ttggatcaat
     6421 agtcagatga gttgcgaaat aatttgcttc tttttcaatt tttaattcag atactagcgt
     6481 ttttttagac aacataggtg tgttttcatt cggatgcaaa acacagtggc ctaattcatg
     6541 actacaagtt atccattttc ccatctcatc taaactagaa tttatgtgga tcatttttat
     6601 ctgtttgaat ttactatagt aaccataaat ttctcccaaa tcttcttcta aaactagtat
     6661 cttcaattct cgtgcaattc taaaaggatt gcgtgtttga tatagtttta ccagcttatt
     6721 aattttgtta tcaatttgag gtaaatacat aaacaacgta ctccttttaa tcgcgatatt
     6781 ttttaggtgt gtattttttc ttagcttcta ttttagctat tcgcaaagca ttctctaaag
     6841 aaataatgag agcttcacgt gtcgcttcac tcatttcatt atctttcttc gaaaaagcca
     6901 ttccatcagc attagataaa tcgtcgatta atgactgcaa ttctttttgg atatcacgct
     6961 cgtctttttc tgttaaatca tagtatcttt ttttctcagt acgtcctagc aaataatcta
     7021 cagatacgtc gaagtagtca gcaatttctt gtaacttttc ggcagttggc tgttttcctg
     7081 cgtttaaact ataaaagtaa ttctctccgt acttcaaatc tttagcgact tgttgaaggt
     7141 ttttattctt gtttttcgct agttctttta ttctttcaaa tgttgtcatt ccaacgttcc
     7201 tttccaacac gaaaaaataa tttacgaaaa agagtaaaaa tagctttaca attttacgat
     7261 atagagttat tatttatccg taagttaatt tgatagaaaa aagcaaacta aaaacacacc
     7321 ttatagcata aagtttggcg accaagtgca aataaaaggt tttgttatag gcttatttct
     7381 ctatgtattc attttactct atagtgtaaa aatgtcaatg gaaattatat tttttctatc
     7441 attttaactt acaaatttat aaaaaaagga agtgggttaa tgtcaaactt ggataacggt
     7501 cgtgcggcga tcaaagagtt tatgaaagag aatggaataa ctatggatga tttggctact
     7561 gcttatggtc acacgcgtgt acgaatgcaa caaattcttg atggacactg gagtggacca
     7621 aaggcaaaca gaactattct agagatcatc cgtgattaca gaattcgata ggaggcaagt
     7681 catgaatatt ctaagcgaag agtttttgac tcgtttaaga attgcaattg ttgaagttgt
     7741 aaaggacgca cttagtcaac tttcaaaaaa gaatttgtca gaaacacgat atttaaaaaa
     7801 gatcgaagct agaaaatatg tgggaggtgt aaacgatcaa ggttttgaga agttaatagc
     7861 tcacggttta aaagaaattc gtatagatgg ctttttaaga tatgacaaaa aggacatcga
     7921 tgaactgatg gctaaataca aaatttaaag gaggtcaaca tgggaaagtt taatcgaaca
     7981 ttagtattca gcgcgccact aattatctac gctttaggtc tatggggtag tagacaagca
     8041 ttaatcggca cgatcgttta catgctttgg attttcattg ggcttgatga agctgagtac
     8101 agagcgaaaa agccagtcgg gagggactga ctaaaaaagt ctaatgtcta taagaaaggt
     8161 ttgacaataa atgaaagaat tatttttttt aataactttc atcatttttt ctatcagtgt
     8221 tttctacatt aacctttgcg ttgctagatt tgctttgaaa agttttagga acagcaaacg
     8281 aaaatagagc tataaaagat acgaatgtag tagctacgtt aaattgctct ttagaaaacg
     8341 tcaatgtcat tggaatgata attgatatcg atgagaaaat ctttgcgata ataactatcc
     8401 atttttcaat tataaacatt tttaaattaa gcaccattag acaaaatgta gttcctatat
     8461 agaggataaa tgaagataga aataaacata ttagtccagt tttaaaaaaa gagaagtaat
     8521 ctatgtgggc aaattctgtt aatatgcttt tttgatttaa cacaatcaaa ctactgttgg
     8581 acaaaaatga cagtaaggaa agagttgctc cgattactaa ataaaaaata gttgttaaca
     8641 atattatatt atcaattttt ctgctcatgt tttcacccca ccagaattat atcaaaaagg
     8701 agagaagaaa taatgcaaga attagtaatt ttgaaaaata aagaagctgt gactacgagc
     8761 ttacaagttg cagaaagctt cgataaaaag cacagacatg ttttgacggc tattgatgaa
     8821 ctaaaagagg gggttgccga aaattgggca gacctatttt gggaagatac ttatgttcat
     8881 cctcaaaata aacaatcata ccgaattatt tatatgaata gagacggctt ctctctatta
     8941 gcaatgggct tcactggtaa gaaagcttta agttttaaac ttcaatatat tgaggccttc
     9001 aacaagatgg aaaaagaatt aaaagaccaa ttgccatcaa ttccagcaac taaacgagaa
     9061 atggcattac ttgctctagc tgcaaacgaa gaaacgaatg aaagagtaga cgctatcgag
     9121 tctgatttaa acgatttgaa gaacaatcaa cttctagctg aaccagatta ccgaacaatt
     9181 tctaatatgg ttcgcaacaa aattagagtt atttgtaatc agcagcattt aaatagcaaa
     9241 gcaaaagcag aattgtttaa agacttgaat ggcggaatta agcgaatcac tggagcagta
     9301 gctagaaatc gtatcaaggc aaaacagttc gatgatgtta tcgattttat taacaattgg
     9361 atgccttcta cagcgacgat gacaattatt aagcaaatgg agttgttaga ggatgaatag
     9421 agctgaagcg ctaaaaatag gcgtaataat tgctaatcgc tggtggagac acaataaacc
     9481 aaacatccta agccaacaac atattgataa gcaaaaagca tggcaacaaa taaaaaagtg
     9541 actcagccga ccaaagcaat gagtcacaaa gaaaatacat ctaaggagat gttaccacat
     9601 gaaaaaagaa ctttccactc tagatcaata tttgactgat cctagttggg gcaaatcgaa
     9661 tatcaaggaa acaagcaatc gaaaaatcag acgtaatctt ttgacggatg aagaactagc
     9721 atgtgatcaa gacgatttgg gaaattttgt gagtatttgg gatcatgtct atcttatcca
     9781 tttatcgagg aagtccaaaa aaactgaata tatctatgtc atcgaagatg gcttgattga
     9841 tgcgctagaa gagtatgaca gagataactt gattgatatc tcttattacg gatcaggtaa
     9901 gaaatacatt gctgaaatgg aggcagaatt tgatgagtga aaaggaacaa cctttgaaaa
     9961 atagaagtga taatacgctt tttaatacct tatacaaaat aaatgtgaaa gatgttactg
    10021 aaaaacgaaa taacctcact tatctttcgt gggcatgggc ttgggcagaa gttagcaaag
    10081 tgtgtgaggc agtagattat gaaatctatc atgatccaga aacatatctg ccgtatgtct
    10141 ttgataagaa aacaggctat atggttttta ccagtatcac agtcaacgga gtaaagcgtg
    10201 acatgtggtt accagtcatg gatggtgcaa acaaggcaat gaaagatgag ccatatacct
    10261 acgaagtcaa tgattatcag tggaataacg aaacgaagaa aaaagagatt gttggaaaaa
    10321 tcgaaaagcg agttgaagca gcaactatgt ttgatat
//
LOCUS       DACJGB010000089         9939 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.091, whole genome shotgun sequence.
ACCESSION   DACJGB010000089 DACJGB010000000
VERSION     DACJGB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.091"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IYI34_002579"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IYI34_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAR0560409.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IYI34_002580"
     CDS             1995..4661
                     /locus_tag="IYI34_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAR0560410.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IYI34_002581"
     CDS             5025..5777
                     /locus_tag="IYI34_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0560411.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IYI34_002582"
     CDS             complement(5799..6953)
                     /locus_tag="IYI34_002582"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAR0560412.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IYI34_002583"
     CDS             7138..9579
                     /locus_tag="IYI34_002583"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAR0560413.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9736..>9939)
                     /locus_tag="IYI34_002584"
     CDS             complement(9736..>9939)
                     /locus_tag="IYI34_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0560414.1"
                     /translation="QSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIKRIA
                     FGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         3189 a         1793 c         2132 g         2825 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttg gctcttcgtc
     9661 aaattgtgtt ggcgaagaca aatttgaata aaacttagtc cgatatgcgg cgcgttctgt
     9721 gcgccgcata tttttttagt tttcaaagat cagaccttgt tgtaaataga tccgtgcccg
     9781 aaagttataa aagttgcgat aaccaaacgc aattcgttta atcagcttga tcttgttatt
     9841 cagcccctca gtgacgccgt ttgagtgggg caccttaaag gcatttgaaa tacccgattg
     9901 gtagcgatgg aagatcgtga atttcttttt aaacgactg
//
LOCUS       DACJGB010000090         9869 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.092, whole genome shotgun sequence.
ACCESSION   DACJGB010000090 DACJGB010000000
VERSION     DACJGB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.092"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(18..263)
                     /locus_tag="IYI34_002585"
     CDS             complement(18..263)
                     /locus_tag="IYI34_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560415.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(377..955)
                     /locus_tag="IYI34_002586"
     CDS             complement(377..955)
                     /locus_tag="IYI34_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAR0560416.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1032..1562)
                     /locus_tag="IYI34_002587"
     CDS             complement(1032..1562)
                     /locus_tag="IYI34_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAR0560417.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="IYI34_002588"
     CDS             complement(1566..2426)
                     /gene="rlmB"
                     /locus_tag="IYI34_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAR0560418.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2419..2814)
                     /locus_tag="IYI34_002589"
     CDS             complement(2419..2814)
                     /locus_tag="IYI34_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAR0560419.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2811..4223)
                     /locus_tag="IYI34_002590"
     CDS             complement(2811..4223)
                     /locus_tag="IYI34_002590"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAR0560420.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4220..4756)
                     /gene="cysE"
                     /locus_tag="IYI34_002591"
     CDS             complement(4220..4756)
                     /gene="cysE"
                     /locus_tag="IYI34_002591"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAR0560421.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(5078..6538)
                     /locus_tag="IYI34_002592"
     CDS             complement(5078..6538)
                     /locus_tag="IYI34_002592"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAR0560422.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6708..7865)
                     /locus_tag="IYI34_002593"
     CDS             complement(6708..7865)
                     /locus_tag="IYI34_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAR0560423.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7899..9269)
                     /gene="radA"
                     /locus_tag="IYI34_002594"
     CDS             complement(7899..9269)
                     /gene="radA"
                     /locus_tag="IYI34_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAR0560424.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9332..9859)
                     /locus_tag="IYI34_002595"
     CDS             complement(9332..9859)
                     /locus_tag="IYI34_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAR0560425.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         2506 a         2213 c         1784 g         3366 t
ORIGIN      
        1 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
       61 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
      121 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
      181 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
      241 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
      301 taaaattttc aagtttacca taaaaaaaaa gaaatgcaat aaaaataaat aagaagctca
      361 ttaagatttt tctaaattat tcctcattat aaaatttcaa gtgttttttt attttgagtt
      421 ttactctccc gtaagcgctc attacttgct tgtgatccat tgctaaacat tcagccatgt
      481 ctacaaaatc aacgccttgc atataataat gaaaaatttt agcttctatt tttgaaaact
      541 ggatttcgca attttcgatt gcttctttta ataaaagatg ctgaatcgct aaaggttctt
      601 gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc tattttttgg tcgattgaga
      661 cttcatcttt ttgatcacga cgcttcagcg cgttctgctt cctcaacaga gaatagattt
      721 gattgcttat aatgctttta taataacttc caaagctaac acctttgctt ttttcgtaag
      781 ttctcaatgc ctgatataaa gcgatccttg cttcttgttc ccaatcctca gaaacataat
      841 ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg aaaatatttt tgatacaggg
      901 cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa taactcctca gacataaaat
      961 aattcctcca taaattcgtt tatggaggaa tcttatcatc aatgattcat cgaactgatt
     1021 agaacgatat gttatttatt ttttctcatt aaatcgtcca atttttggga caattttttt
     1081 aactgctctt cattccatgg agaattacgt cggaaatcaa taaaatgcat atctttattt
     1141 tcttgggcta ttgttttttc cgtttttttt atcgtcttat ataattcata agcagaagtt
     1201 cgtaatgctc cttgggaaaa gatcatccac tgttctgcta aatcgcttgt cgccactgtc
     1261 acttgtgtca aacggtcatt cagctgacca gcgattctct cgatataact gtcagccgtt
     1321 tcgtcttctt tcgtaaaaac aacatccaac tggtatttct tataattttg ttggatgccc
     1381 ggaactagtt gggcatcgaa gacaacgatg acttccattc cttcatattt cgcataattt
     1441 gataaacgat gtaaaagagc ttctcttgca tccgccattt tgtcctgctt tttaagctgg
     1501 acaagttccg gccacgaacc aatcatgttg tagccgtcta caatcaacag ctgttttttc
     1561 atggtctact ccccctgacg ttgggaaaaa gcctgataca taagcaaacc agcagcaaca
     1621 ctggcattta agctctggac gtgcccggtc atcgggatcg tcagcaattc atccatttct
     1681 tttttcaatc ctggactcat tcctttgcct tcattaccaa tgactaaagc aattgatcct
     1741 ttggcattcc atttttggaa gtttgtgccg gtcatatctg tgccaaatac ccaaaatcct
     1801 gtttctttga gctgttgcac agcttgtacc aaattcgtca cgcgtgcaac agggacatgt
     1861 tcgactgccc cagtggaagt ctttgttacg ataggtgtga ttccaactgc tcggtgtttt
     1921 ggtatgatca caccatcgac gccacttgcg tctgctgtcc gtaaaatcga cccaaaattg
     1981 tgtggatctt cgatgctatc caaaatcaaa aagaaggggt cttctttttt ctcttttgtt
     2041 cgttcaatca gttggtcaag ggataaatat tcataaggtg taatcgcaag aacgactcct
     2101 tgatgcacgc cgtgatcgct caaagtttct aatttttgtt taggaaccca tttgactggc
     2161 acagcttgtt cacgtgcgat ttctttcagt tcttctactt tttctcctcg tgaatcttct
     2221 tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt ctactgcggc atggtgccca
     2281 aagacgaaat tatcttctaa tgcttcttca ttttgatcag aaactgactg atttcgtcca
     2341 gtttgctttt tatttgaata tctattttgt tttttgtctt caaatccacg tttcgattga
     2401 tgtttgtttg ttctttcttt acccattttt ttcaccaact ttctgaatac accagcggat
     2461 caattcttcc atccgctcgg tttgttttgt caaatgcaga tagcccatga gcgcttcgaa
     2521 ccctgttgcg acccgatacg tcgtaacatc agcatttttt gcgctcgtat ggcttttagc
     2581 atttcttcct ctgcgataat aaagttcttc ttcttctgtc aaaagatgtt cttccagcat
     2641 ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa ttcgttgccg cacgatgcag
     2701 cttattgggt tttgtttctc cttgatcaat caggtggtca cgaacataga tttcgtaaat
     2761 cgcatctcca acataagcca aggctaaacc gttcaattgt gtgtaatctc tcatgcttct
     2821 cttctccatc gggttccttg cggtgtatct tctaataaga tccctttttc tttcaacaaa
     2881 tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc ttgcttgatt tcgttcttcg
     2941 atcaattgtt caatctcttc atctaataac tcttctgctg taaataaaat accgaagatc
     3001 gaaagccatt gcgagaataa agcttctcct tgtgccaaga cagcggcaga aactttcggc
     3061 tggtctagat aagtgttcag ccacttagca agctcataga ccactgtgat cccatttgct
     3121 gcattaaaat catcgtccat ttcttcgatg aatcgctgtt ctaaattttc taacaaagcg
     3181 attttatccg catcatctgc caaattctct gaggcagttt cttggcggaa tttcaaattg
     3241 tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt ctttcatcgt cgtttcactg
     3301 tagcggatcg gacgacggta ttgtgtggtc gccatgaaga aacgcaagac ttgtggatca
     3361 acgtttttga tcatttcatg tacagtgatg aaattcccta gagatttact cattttttca
     3421 tcgtcttcgc caatcgttac atagccattg tgcatccagt aattagcgaa ttttttccct
     3481 gttttcgctt cactttgtgc gatttcattt tcatgatgag gaaattctaa atcttgcccg
     3541 ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg ccataacgga acattcaata
     3601 tgccaaccgg gacgtccttt gccccatggt gaatcccaag aaatctcgcc aggttttgct
     3661 tgtttccata aagcgaaatc taatgggtct tctttttgct gctgttcttc ccctgtacgc
     3721 tgacttgctc cgacttccag ttcatcgatg gattggtcac ttaatttccc ataattttca
     3781 aatttacggg tacggtaata cacatcacct gaagattcat aagcatagcc tttttcaacc
     3841 aacacttgaa taaatgctaa aatatctggc atatgatcca tgactcttgg atgcagtgta
     3901 gcaggctcga cattcaacgc tttcgtatct tcttcaaatg ctcggatgaa acgatcagca
     3961 acttctggtg ctgtaatacc taattcgttt gctgtacgga tgattttatc gtctacatcc
     4021 gtgaaattcg atacgaaatc gactttatag cctcgatatt caaaatagcg tcgaattgta
     4081 tcgaaagcaa tggtgctacg agcatttccg atatggatat aattatacac agtcggtccg
     4141 cagacataca taccgacatg tcccttttgt atagggatga attcttcttt ttttctggtc
     4201 aatgtattgt agatttttat cattctgatc tcctcacttt ttctcctttg attcgaacga
     4261 cttttgctgg tactcctacc gctgtagcgt cgtctggaac atctttcaac acaacggcac
     4321 ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc taatatctgc gcattcgcag
     4381 agagcatggc tccttgttta acagttgggt gtcttttacc cgtttctttt cctgtgccgc
     4441 ctaatgttac gccatggaat aaaatcacat cgtcctcgat ttctgcggtt tcgccaatga
     4501 cgattcccat tccatggtcg atgaacacgc cagttccaat cgttgctcct ggatggatct
     4561 caacaccagt taggaaacgc cagaattgag cattgatctt cgccaataaa aagagccggt
     4621 gctgatataa aaaatgagag aaacgatgcc aaaacaaggc atgaaaacca gggtatgtca
     4681 aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct ctttgctgca tcaatcgctc
     4741 gggtaaacca actcatgact ttccactcct ttcgtaagaa ttttatgtag gaaatcatct
     4801 tcttataaag aaaagaagcg ccttttaaat aagtttcctt atcaaaagac gctttccgcg
     4861 tggttccact tttcttcata atcaatgatt atctctgaca gctaacgcct gttatacgtt
     4921 cttggctact ttgtttccta taaaacagtt tttcctgttt tctgtgtttc gccaagacca
     4981 ctcccagagg catttcggca accattcatt gataccttgc accacccggt atctctcttg
     5041 taatgatcag ctgcttactt ttttctgttc atcgtgttta gttcagtact tgatttaaat
     5101 gtgctaatga tttttcacga ccaagcaatt cgatcgtttc aggcaattct ggaccatgca
     5161 tttgtcctga tacagctaca cggattggca tgaaaagatt ttttcctttt acgccagttt
     5221 ctttttgaac agctttgatg gcagctttga cagttggagc atctactact tccatttgtt
     5281 ctagctgtgc tttgaatgcg tggagaacag taggaactgt ttcaccagca agtacttctt
     5341 ttgctgcttc attcaataca ggatgttcat tgaaaaatag cgtagacaga tcaacgattt
     5401 ctgcagcata gctcatttga ggttgataca ggctaacgat cttcttcacc cattcgattg
     5461 tcttttcatc tgggttttct gctacacgtc catcagcgat tagataaggc agacacatgt
     5521 cagtaaatgc atctagatcc attgctttga tgtaatggtt gtttacccat tgcagttttt
     5581 tcgcatcgaa agctgcgggt gatttactta ggcgttttgg atcgaacatt ttgatcaatt
     5641 cttggtgttc gaagatttcg tcttcgccca caggtgacca gcccaataat gcaataaagt
     5701 tgaacattgc atctggtaga tagcctaatt cgcgatattg ttcgataaat tgcaagattg
     5761 attcgtcacg tttgcttagt tttttacctg tttcactgtt gatgatcaat gtcatatgtc
     5821 cgaaggttgg tggtgtccag ccaaatgctt catagatcat cagttgtttt ggtgtattgg
     5881 caatatggtc atctccacgt aaaacgtgag tgattttcat caaatgatca tcgaccgcaa
     5941 cagcaaaatt ataagttggc ataccatcgc gtttttgtat gacaaagtcg ccaccgatat
     6001 tatcggattc gaacgaaata gcgcctttca ccatatctgt aaatgcgtat tctgtattgc
     6061 gaggcacacg gaaacgtaca acagattcta gaccttgtgc ttctttttct gcttgttctt
     6121 caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat ttctcctctt gcgcgttgtg
     6181 cttcccgttc tgcttctaat tcttcttctg tgcaatagca tttgtatgct cgattgctag
     6241 caagcagctg atcaatcagc ggttgataga tttctttacg ttcagattga cggtaagggc
     6301 cgtattctcc aggattttca ggagattcat cccaattcat tcccaaccaa gcaaggtttt
     6361 ccaactggct tttttcgcca tcttcgatat tacgtttttg gtcagtatcc tcaatacgaa
     6421 taataaagtc tccatcattg tggcgggcaa acaaataatt gaataatgca gttcttgcat
     6481 ttccaatatg caggtgccca gttggacttg gtgcatagcg tacgcgtact ttcgtcattt
     6541 tcttcctctt ctttcaaact agactttgac taacagcaaa aattccgtca gttacggctt
     6601 ctcaaatatc atttatttaa agccactcag cacaaatttt accttttcta aggtaaatag
     6661 taaagccaac tggttgaatt tccttaccag ttggctgaat ttctttttta ttttgattgt
     6721 ttagtctttt tctctgtctc ttttttattg tcgattcctc gtcctgcatg tgcaggttta
     6781 gcaaagatca tgcgtccagc agctgtttga agggcacttg ttacaacgac ttcgatacgt
     6841 tcattcatgt aatgctgtcc atcttctacg acaaccatcg tgccgtcatc aaggtaggct
     6901 actccttgtt ggcgttctgt cccggctttt actactagaa cattcatcgt ttcaccaggg
     6961 atgacgactg gttttacggc atttgccaac gcattgatat taagaacagg tacattttgg
     7021 aattcagaga ctttattcaa gttatagtca ttggttacaa tgacaccatc taaaagctta
     7081 gccaatttga tcaatttgct gtctacttca ctgatatctt caaagtctcc atcgtacatt
     7141 tcgacagata taccctcttc tttttgcaag gcatttagga tatctaatcc gcgacgacca
     7201 cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt gtagttcgta aagaacaaaa
     7261 ttagggatca tcaaggttcc ttctaagaat ccagtctttg taatatcata aatccgaccg
     7321 tcaatgatca cgcttgtatc caagatttta tatttatgga aattttctcc tgattttcgt
     7381 tctaacagtt gactctcaac ttgactagct gtttcttgct cgattttttt gcttcgtgaa
     7441 ccaaaaactt tcttccattc ttcgattcgt gtcgttccca ttctcaatcc cagatagcct
     7501 aagatgatca tcagtaaaat cggcaataca ctattgacga atggaatttc catattgtaa
     7561 agaggggtcg agatcacaac acctaaagtc aatccgatga tcgcaccgat cgcaccgaag
     7621 agaagataag tcaagctgac ctcacttagt ttttgttcta cttgtttgat aatagctgag
     7681 atatgtttgg ccaacagcaa agataataaa aagaaaataa gtgcgcctaa caggctgttg
     7741 gtaaatgtat tattcagcca tccgtttgac tgttgaccga ttgcctgcca agctgagggt
     7801 aaaaatgaaa tacccaagct tgcgccgaca atgatcatca gcaaagtaat gacacgtttt
     7861 tgcatcgttt ttcctccgtt tcgacaatca tttattagtt agcggaacac acgcttcaac
     7921 gtttcagcca aagttgctac tccaactatc tcgattcctt ttggcgcttc ccagttacct
     7981 aagttgtttt taggtaggta tacttttgtg aagccgagtt tttggacttc tttgactcgt
     8041 tgttcaatat gactgacacg tctgatttct ccagtcagtc caatttcacc gatgaaacat
     8101 tccgttggct gtgttccttt ttctttatag cttgaagcaa tactgacagc gatagctaaa
     8161 tcaatcgctg gttcatttaa ttttacccca cctgcagctt ttaagtaagc atcttgattc
     8221 tgaagaagca acccagctct tttttccagc actgccatga tcagagatac ccgattgaaa
     8281 tcaagacctg ttgttgttcg tttggcattt ccgaacatag taggtgtgac taatgcttgg
     8341 atctctacca aaattggacg tgtcccttcc attgccacaa cgatagcaga gccagttgca
     8401 ccgtctaggc gttcttctaa gaaaatctgt gaaggattag cgacttcttc caatccatgc
     8461 tcatgcattt caaaaatccc aatctcattc gttgagccaa atcggttttt tactgctcgc
     8521 aaaatccgaa aactatgatg ttgttcacct tcaaaatata acaccgtatc caccatatgt
     8581 tctaacattc ttgggccggc gatcgagcct tctttggtaa catgaccgac gataaaaatg
     8641 gctatcccat ttgttttagc gattttgagt agttctgcag tggtttctct tacttggctg
     8701 acacttcctg ccacacttgt gatgtctggc tgcgtcatcg tttggatgga atcaatgata
     8761 acataatcag gggagatatg ttcgatggca cgactgattt cgttcatatc tgtttcagcg
     8821 tatagataaa attctgtgtt gatacttccg aggcgttccg cgcgtaattt tatctgctct
     8881 gcgctttctt cgccggatac atataagact tttccgccta tggctgctaa ttgctgagaa
     8941 acttgtaaca gcaaagtgga tttcccgata cctggatcgc caccaatcaa gaccatagac
     9001 cctggcacca cacctccgcc taacacacgg ttcagttctt ctaattttgt tttgatccga
     9061 ggttcttttt tagggacgac gtctgcgatt tttgttggtt ttgctttttc tcctgtcaaa
     9121 cttgtccgag ttctgcggtc cgttgtatct tgttcgattt cttcgaccat cgtattccat
     9181 tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca catacccgca attttggcaa
     9241 acaaattgta cttttgattt ttttgccatt gtaccgcctc cttttatcct aatcatgaga
     9301 cattaaatca agcttaagta tctttaacgt tttatttact ggaagaacca aatccgcctt
     9361 gacgttgagc agtcgtttga tcttgatctg cttttaaaaa cggcaaaaag atcccttggc
     9421 cgatacgctc gccttttttg atcactacat ccttgaaacc aaagttggtg aattggaaca
     9481 tgatatggcc ttcatttgcc tcattattgt aataatcact gtcaatcaca cctactccgt
     9541 ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg gttagcaagc tgcaaaaatt
     9601 catcttctcc catgtaagct ttgattccag taggcacgag ggttggtttc aattgttttt
     9661 cattttctga tacatctttt ttcaatccat ctgcgactgt ttccaacccc tttttccagc
     9721 ctgctttcca gatacttgga atcacgacct cttctgctgc ttcaaaatca tagccagcag
     9781 catgatgtgt agcgcgctgc ggtagattga tattttgatc tttatattta gaaataactt
     9841 caaatcctct ttttctcacc ggttttcct
//
LOCUS       DACJGB010000091         9811 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.093, whole genome shotgun sequence.
ACCESSION   DACJGB010000091 DACJGB010000000
VERSION     DACJGB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.093"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(34..2154)
                     /locus_tag="IYI34_002596"
     CDS             complement(34..2154)
                     /locus_tag="IYI34_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR0560426.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="IYI34_002597"
     CDS             2384..3562
                     /locus_tag="IYI34_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAR0560427.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="IYI34_002598"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="IYI34_002598"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAR0560428.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="IYI34_002599"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="IYI34_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAR0560429.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="IYI34_002600"
     CDS             complement(4915..6168)
                     /locus_tag="IYI34_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR0560430.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="IYI34_002601"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="IYI34_002601"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAR0560431.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="IYI34_002602"
     CDS             7746..9092
                     /locus_tag="IYI34_002602"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAR0560432.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="IYI34_002603"
     CDS             complement(9400..9786)
                     /locus_tag="IYI34_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR0560433.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3127 a         1782 c         2011 g         2891 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactag tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttgtctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DACJGB010000092         9664 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.094, whole genome shotgun sequence.
ACCESSION   DACJGB010000092 DACJGB010000000
VERSION     DACJGB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.094"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..650)
                     /locus_tag="IYI34_002604"
     CDS             complement(<1..650)
                     /locus_tag="IYI34_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR0560434.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIG"
     gene            975..1391
                     /locus_tag="IYI34_002605"
     CDS             975..1391
                     /locus_tag="IYI34_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAR0560435.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1388..1858
                     /locus_tag="IYI34_002606"
     CDS             1388..1858
                     /locus_tag="IYI34_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAR0560436.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1872..2645
                     /locus_tag="IYI34_002607"
     CDS             1872..2645
                     /locus_tag="IYI34_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAR0560437.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2659..3471
                     /locus_tag="IYI34_002608"
     CDS             2659..3471
                     /locus_tag="IYI34_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAR0560438.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3525..4490
                     /locus_tag="IYI34_002609"
     CDS             3525..4490
                     /locus_tag="IYI34_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR0560439.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4606..7329
                     /locus_tag="IYI34_002610"
     CDS             4606..7329
                     /locus_tag="IYI34_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0560440.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7342..7974
                     /locus_tag="IYI34_002611"
     CDS             7342..7974
                     /locus_tag="IYI34_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAR0560441.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8873..>9664
                     /locus_tag="IYI34_002612"
     CDS             8873..>9664
                     /locus_tag="IYI34_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR0560442.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         3260 a         1477 c         1884 g         3043 t
ORIGIN      
        1 ccaatcatat ggaagccacc gattaggtat tttatgggtt tagtcggaaa tgtttcaaga
       61 gcaatttcca tcatatcgat aatgccatga tgactacagc cagtaaagac aatcagaccg
      121 tcttcttgct caattacgac caataattca tctgaaaaat catcataaac aaatttatca
      181 tccatctttt tgtataaatt cttggtggtc gagtttgctt tgcctagggg gatgatatgg
      241 aagctcggag caagttctgt tttcgttttt attgtatgta aattctcatg cattttaagg
      301 atttcatttt taggaatacc aacatacaga aacgtggtta aaatctttaa ataatggggt
      361 aaagttacac cttcagctgt ataaataggg gcgtttcctg attttaaata tcttaacccg
      421 ccggtgtggt cagaatgacc gtgtgaaata atcactgcct ccagatcatt caaagtttcc
      481 cccattttag tcgcattata gactaatttt ttattagcgc cagcatcgaa gagatatttc
      541 ttccctgagt agttaattaa gagagataac ccatgtccac ttttaaagtt caaatttatt
      601 cttgagtttt caaataaaac cttgatttcc actataacat ctcctttcac atgaacgtgt
      661 attcatatat tgtagcacat taagttctat tttttgttgt aattaccgaa aaaaatatta
      721 atggacgctt tttacgcttt atttcgtatg tggaagaaaa tttcttagga tgtggcgggg
      781 gattggggca aagctaaatt ccaattacag tgatcattta attagacacc tttaaatacc
      841 cacactttca tcaacactat aatttaggga aagaaaagtg ttggcatgat atagaaaaac
      901 ctttaattaa cagcgtttcc taaaattggc acgcatcttg cttatagaaa agcgtaagga
      961 taggaggaat gaaaatggca actataattc ttgccagcca tggggaattt gctcaaggat
     1021 taaagcaaac agctacaatg attattggag atagtgtacc aattcatgcc ttgtcagcat
     1081 ttagagatga agatgaatct attttggtac aaataaaaaa acttttagcc acaatagata
     1141 tagaagaaac ctacatttta actgatattc taggtggtag tgtgaataat gatatgctca
     1201 ctattattaa agagtatgag ggattgcaat taattactgg aatgaactta cctttagtta
     1261 tttctattgc aacctataat ggaaaaattg gggaaagtga gctagaaaag cttattaaag
     1321 agagtcaaca atcagtgatt gattgtagaa aattattagt agaacaactt actggaggag
     1381 atgacctatg attaagttag tacgtattga ccatcgtttg ttgcacggac aggtagtatt
     1441 ttcttggagt aaatcattaa atatcaatcg tattattatt gccaataaag aggcagcgaa
     1501 tgatgaattc aaaaaaatgt ctttgaattt atctaaaccg actggcatta aattaacaat
     1561 tttttcagtt gaacagttac tagaaaaaat gcctaaaatc gaaacactta acgaaaatat
     1621 catgcttatt tttggtaata caaaagaaat tcgtcaattt tgtgaagcct atcctaaagt
     1681 acaagaaatc aactatggag ggataatcaa aaaagaaggt tccaagcaat ttagtaatgc
     1741 cattttcttg aatgaatctg agcaagaaga tgccaaaaaa ttaaaagctc ttggcattaa
     1801 acaatttatt caacaggtac caacttctaa aaaagaagat ttgaatacaa tgttataaag
     1861 gaggaatgaa aatgctcgtt cagtcaattg gtttagggtt aatcggtgtt ttttgtattt
     1921 tagactcaag gcttttagga agaatgaatt ttgaacgacc gttgattgtc agtacgcttg
     1981 ttggtttggt acttggagac atggaaaaag gattaattgt tggagcctct ttggaattga
     2041 tgtccttagg attagtaaat atcggagctg ctgcaccacc tgatatgaat atggcatcaa
     2101 ttattgctac agcctttgcc attttatcaa atgcgaatgc agaagcagca ttaacgattg
     2161 ccattccaat ttcagtgtta ggtcaaatac taggtatctt aatgcggacg attttgtcaa
     2221 atttgactca cgttgcggat cgctttatcg aacaagggaa atttagccaa gcaaggagaa
     2281 ttcatatact ttatggctcg ggattatatg ctctcatgta ttttattcct atttttctag
     2341 caatttattt tggaacgaat cttgtttctc agattgttga tgcgattcct gagtggctaa
     2401 caaatggctt aacggttgca agtaaaattt taccagcata tgggtttgcg ttgttattac
     2461 aaacgatgtt aacgaaaaaa atggtgccat ttttattact tggattttta attactgctt
     2521 attctggatt gggaatcatt ggtattgcag ccttcgcatg tattgttgcg tttgtcatgg
     2581 ttcaagttca aggtaaaaac tcaaatggta accaaaatga gccagatcca ttagatgact
     2641 tataaggagg tagattaagt gaaagagaaa gatcaaaatg catggaaata tcggcagttc
     2701 ttatggcgtt catgggcaat tcaagcctca tggaactatg aacgacaaat gaacatgggt
     2761 tttatgtacg gaattgcacc aattctagat aacatttaca agaatccaga agatgaagca
     2821 atgaagaaag aagcttacga gcggcatatg atgtactaca actgtacccc tcaaacaagt
     2881 gcatttgtgt tagggttaac tgcttctatg gaggagcaat actacgaaga ccaagaaaac
     2941 tttaatccgg aatcaatcag tgcagtgaag acttctctga tgggaccgct atctggtgtt
     3001 ggtgattctt tctttcaagg aacgattcgg gtattagcct ttggactagg aattaattta
     3061 gcccaacaag gtagtatttt aggtccaatt ctagcaatgt taatttcctt tgtaccttcc
     3121 tttttagtta cttactatgg tgggaaaatt ggctataaca tggggaataa atacttgtct
     3181 aaactgtatc aagaaggttt gatggataaa gtaatgtatg tttgctccat tgtggggttg
     3241 atggtcattg gttcgatgat tgccagcatg atcgggatta caacaccaat tcaaatgggt
     3301 aaagcttttg tcttacaaga tgtattagat agtattatgc cacaattttt accattagga
     3361 ttaaccttct tcatgtactg gttattgcag aaaaaggtca aaacaggatg gatgttggca
     3421 atctgtttag tcggaggaat tatttttagt ttgttaggta tcttacaata agggaataac
     3481 caataaattt ggttgaataa tataaataag gaggaattca attgatgaat tcaaaagaaa
     3541 ttgttaaaga aatcgtagcg aaaaaagagg taaaatcaat cgtatttgtt ggctgtggcg
     3601 cttcaaaagc ggacctttat cctgcaaagt attttctaga ccaaaatgcg aagcagttac
     3661 ggattagtca ttacacagcc agcgaattta actatgcaac accagcatct gtagatgagt
     3721 caactgtcgt tatttcagct tctttaggag gtacgactcc agaaacagtt gaagctaacg
     3781 cagtagctaa agaaaaaggt gctacagtta ttagtctaac acgaagcatt gactcaccgt
     3841 taacaaaaga tgcagattat gtaatttatc atcgatttgc agagaactat gctgctaaat
     3901 tagaaaaaac aggctactgt ttagaattgg cagtagaact tttacatcaa gtagaaggtt
     3961 atgaccatta tgaagcaatg caagaaggat ttgctactat ctatgatctt gcccaaaaaa
     4021 gtgcagaatc agctcgtaaa gatgcgaaga aatttgctga acgctttaaa gatgaaaaag
     4081 ttatttatgt aatgagtagt ggtgctacac atgaagtagc atactcaaca tcaatttgct
     4141 tgatgatgga aatgcaatgg attaattctg gtaccttcca ttcaggagaa tttttccacg
     4201 gaccattcga aattgtggat aaagacgtac cgtttatatt attaatgaat gatggaaaaa
     4261 ctagaaatat agatgctcga gcattaactt tccttgaacg ctttgatacg aaaacagaag
     4321 ttgtagatgc cttggattat ggcttgtctt cacatattgc taaagaagtc ttagactact
     4381 ttaacccatt cgtgattact gcagtattta gagtttatgc agaagaattg tcagaagcaa
     4441 gagaacatcc attaactaag agacgttata tgtggaaact aacttattaa aaaatagatc
     4501 aaactaaata aggtggcttg agaaattaag ccaccttgtt ttcttcaatg tgattttata
     4561 catatggtaa aataactagt acatattgtg aaagcaggtg gcaagatgca gcgattaaat
     4621 gactatcttt atcaactaac attgcttttg aaatctacgc cttcagataa ttattcagct
     4681 cgcagtattt cgcaagcttt acagttgaat agaagtacaa tcagtagcta cttaaatgaa
     4741 ggtgttcgtg aagggctttt cataaaagta aaaagttacc cggttttatt tttacataga
     4801 acagctcttg aagaactaca tatcacactg aataactccg aaatagaatc aattgaaagc
     4861 ttgttaacaa tttctcaaaa acccgcatta gatcaagtaa ttggctctaa aggaagtttg
     4921 aaagaagcaa ttgatcaaat taaaacggca gtcttatatc ctggaaaagg gttgccttta
     4981 ttattgatcg gcgctagtgg atcagggaaa acctttctag cgaataaaat atatgaatat
     5041 gcagtagaag aaaaggtcat tcacacatcg gcacctttca tagattataa ttgtgcacaa
     5101 tatgtgagca atccagagtt actatcctct gctttatttg gctacacgaa aggagcattt
     5161 accggagcaa gccaggagca tactggtttg ctagaaaaag ccgatggagg agtattgttt
     5221 ttagatgaag tgcatcgctt atctgaagaa gggcaagaga aattatttac atttatggat
     5281 acgggtgaat tctctcctat gggggataat agtattcgga aaaaagccga tgttcgatta
     5341 gtctttgcaa caacagaaaa tatatatacg actttcttac caacttttct taggagattg
     5401 ccagttattg ttaatctacc tagatttcaa caacgtccgt cgtttgaacg tctgtcattg
     5461 attgatgaat tttttgtttc tgagagtaaa attttagcta aggaactatc tgtttctgat
     5521 gcattgattc atttcttaat gaatagtgat tttgaaggta acgtggggaa tattaaaaat
     5581 attatcaagt atgcttgcgg aaacgcatat atccaccaga agagtcagca aacaattcaa
     5641 gttaaattat tggatatgcc tttagagtac agttataaat tcaaggagca actaaataaa
     5701 cccagaaaga aaagagcaga tcgaatctac tatccagaag aaacacgaca agtgcaacta
     5761 gaaagaaaca atcagctttt tgttcaattt tttgaccaat tacttactgg attctctgaa
     5821 gtgattgaaa ataacaagga tattaaacta tttttagaag aaatggttac caaggtaaca
     5881 caagtaatgg atacgcttat ttttcaagag gattacgaaa aagagcagtc tttatacgcc
     5941 attctaaact atcatattcg tcaaagtatt gattttatgc gagaaagcta cgggtttgaa
     6001 caagatggta accgggtagt cgctttggcc aactatctat atgtcaagga taatcaagtg
     6061 atttttgaaa atagtcttca agaaaagaaa accaaagatc agcttaacga attttttgct
     6121 aatgttcttg aaacccctta ttggtatgca aaaaaaatct taagcctttt atctgagtgt
     6181 ttagatcaac ctgtgtatga agaggacatt ttctttatta cgctatattt ttatagtttg
     6241 aatgtcacga atgttaaaac aggtattaag agtattgtgc tagctcatgg ttattctaca
     6301 gctagtagta tggcgaatgt tgccaaccgt atgctgaata agaacgtttt tcaagcttat
     6361 gatatgcctt tagatattac attagagaaa attgaagcaa aattgattca attccttgga
     6421 gattatcgaa cagattcagg cttgatttta ttggtagata tgggttcact aaaccaacta
     6481 ggcggtttat tagtgaatca tctaaatgga ccactgttaa tcattgatca tatcaataca
     6541 ccgttattat tggaagtcgg ccaacaagta ttaaacaata aaagtatcac cgaaatagga
     6601 gcagaaattg aaacaagtct tactttcaaa aagcagttac tacttcccaa gaaaaagaaa
     6661 aagaaagcca tcattacttg ttgttataca gggattggta gtgcgaccca gattcaggag
     6721 attttaacga aatgcttgaa agatgcagtt agagaattag taattattcc gtatgattac
     6781 aaaaagctgt tagaaaacaa gaattttgag actccttttc aattatacga tgtcatcatg
     6841 attgtaggta cagaagatcc caaaattaat cagattccat atattggatt agatcgcctg
     6901 attaatgggg aagcaattgg cgaatttgtt cattacttgg aaaaagagct gactattgaa
     6961 gtgaacgatt tacaaaaaga attaattttc aatttttcaa ttagtaaaat tgtcgaaaat
     7021 ttaaccattt tggatccaga taaggtgcta tcttttgtac aaaaagcaat taaagaatta
     7081 tcgaagttat taaattttga tttttctaat aatcagctgt ttttgttgta ccttcactgt
     7141 agttgtatga ttgaacgaat tgtacgtaaa gaaagtgtgg atgagcaaga ggacattgaa
     7201 gaatatatgc aagcaaatgg agaaataatg gataaaattc atagcgcttt ttttgagatt
     7261 gaaagggaat acacaattga aataccagat ttagagttgc gattattgag cgatattgtt
     7321 caaggatagg agtgaattcg aatgacaatg ggagtaattt ttgatatgga tggggtactc
     7381 atagacagcg agacatttta ttttgagaga agaatgaact tctttcaaga actggagata
     7441 gatccaggat caaaccagat tgatgattat gtggggaaaa cagaagctgc aatatgggaa
     7501 acattagttc ctgaggacaa atcattaaga gaggaacttt atccgaaata tgtagattat
     7561 cgaaaaacgc atccaattga tttcgctaaa gatttacgaa aagaagtgaa accgttactt
     7621 gacttcctca agaagcggga tataccattg gcactggcat cttcttctcc agttgttgag
     7681 atagagagta tgctcattca atgtgaactt actgaatatt tttcctttgt tataagtggt
     7741 gagtcattaa aacaaagcaa accacatccc gaaatatatt taaaagcgaa acagcaatta
     7801 gactgtgact tctttattgc agtagaggat tcaacgattg gtattaaatc agcaaaggca
     7861 gcaaatatct atacggtggc actaaaacaa ccaatgcaaa tcgatcagag tgaagcagac
     7921 atacaaattg aaagtctaca agaactggaa caaattgttt tagaaaaagg ttagtgaatt
     7981 aatggaaata aggctatata tctgtgctag ctattttgac taaccaaaaa aacttgctat
     8041 aattcaggtg tagtaaaagg tgctggtaac accttttacg aaaatgcttc ggcagtgata
     8101 atgggattgg ctttttaagt tttttatttt gttcgcaatc cctaaggtca tgtgaatgat
     8161 cctagggatt gctagaatat tttgcaacat tctatctatt tcagaacgta accttcccca
     8221 gttcattggg attcattagc atacaaccaa catttcaaaa agaaattgca ctacaccatt
     8281 attagctatt ttgactaacc ccaaaaaact tgctataatt caggtgtagg taaaaggtgc
     8341 tggtatcacc ttttacgaaa ctgcttcggc agtggtaatg agtgtggctt aaaggactag
     8401 tcctttaagc tttttacttt gttcgcaatc tctaagatca agttaatgat cgataagatt
     8461 gctagaatat tttgcaacat tctatttcag aacgtaacct ttcccattcc actgtgattc
     8521 attggcaacc ctcctcacaa ccaacgtttc aaaagaaatt gtaaagttgt tggggtacca
     8581 cgaccgaaca atactacacc tgaatgatag taagttgtct cattaagtat agcatatcct
     8641 ctttttgggt aaatagaaat cggttacatt ttttatccaa gaccaagttt tttcatttgg
     8701 tcttatttgc tatacccaaa caattccgca gtatagtcga cggtattgat tttatcaggt
     8761 gagtaataga aacttgtttt ccttccccaa aacattacct ataaaggaga aaaaatggta
     8821 tcgtaattat ataggtatag gatgaaattt ttttctaaga ggagggttgt tgatgaaaaa
     8881 tttacagtta aaatttattg ttgatccatc gattgtacgc tggtttcgga ttatcaatca
     8941 atttgagcgc aatcaacttg ccacaaaagg agaattagca aagagtaatc atgtatctga
     9001 acgaaccatt caaacagatg tcaataaaat gaaggactac ttttctgaaa gtgttcaatt
     9061 tgtttcaacg aaaagtgggt attcttttca gaaggaaaga ccggttcttt ttctcaagca
     9121 gaaggagcaa ctactggaga acgaaatttt atttgagttg ttagggaatg tcttttatgg
     9181 cgaattggaa gatatgacgg agctaattga tcgttttcat ttatctgaag cgacttttcg
     9241 tcggtatttg aagcagattc aaccagtttt ggaagaatac ggcttagaat tgagtcttta
     9301 tcctcttgat ttaaaaggag aagaggcaaa tattcgcaag ttttttaaag acttctatta
     9361 tgaaggggag atgacgcccc atactcttgt tccaccaagg gatcttcatg aacttgtgca
     9421 agctacattc tatgataaat ttgagtattt ctcgattgga acagggactt ctcctgctga
     9481 attttattat agtttgtata ttgcgattga gcgtgtgaga caaggaaaaa cgatagccat
     9541 tcctcaaggt ctgattgagc gagtaattgc tagtgagaat tttcgcttaa tgtattcctt
     9601 gaaagaagcg attcaaacaa attatcaagt tgctttatca gaagaggagt tttgttggtt
     9661 gtat
//
LOCUS       DACJGB010000093         9556 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.095, whole genome shotgun sequence.
ACCESSION   DACJGB010000093 DACJGB010000000
VERSION     DACJGB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.095"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..345
                     /locus_tag="IYI34_002613"
     CDS             <1..345
                     /locus_tag="IYI34_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560443.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
     gene            426..1046
                     /locus_tag="IYI34_002614"
     CDS             426..1046
                     /locus_tag="IYI34_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560444.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            1747..2031
                     /locus_tag="IYI34_002615"
     CDS             1747..2031
                     /locus_tag="IYI34_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0560445.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            2034..2951
                     /locus_tag="IYI34_002616"
     CDS             2034..2951
                     /locus_tag="IYI34_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAR0560446.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            2944..3726
                     /locus_tag="IYI34_002617"
     CDS             2944..3726
                     /locus_tag="IYI34_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR0560447.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            3727..4407
                     /locus_tag="IYI34_002618"
     CDS             3727..4407
                     /locus_tag="IYI34_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560448.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            4400..5296
                     /locus_tag="IYI34_002619"
     CDS             4400..5296
                     /locus_tag="IYI34_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0560449.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            5491..7140
                     /locus_tag="IYI34_002620"
     CDS             5491..7140
                     /locus_tag="IYI34_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAR0560450.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            7734..8669
                     /locus_tag="IYI34_002621"
     CDS             7734..8669
                     /locus_tag="IYI34_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0560451.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            8662..9432
                     /locus_tag="IYI34_002622"
     CDS             8662..9432
                     /locus_tag="IYI34_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0560452.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
BASE COUNT         3056 a         1584 c         2040 g         2876 t
ORIGIN      
        1 tcaaatataa aaactagtac gaaattaatt tgtaaattat ttaagcttga taaaataagc
       61 aatatcgatg atgtaaagtc caagatatta acaagaaaag taagcgaacc agtaaagcta
      121 acggcacaag ataaaaaaaa tggaattgtt ctttatggtg atgatgcaaa gtataactat
      181 aattgggata cctcctcaga agctgaggta ttaagtattc ctactaccga tacttcaggg
      241 gaaaaagtca cctttaaaat aaattttata acggcaattt ctaagttgga gtatgggaag
      301 caaaaaaaga gagataacaa tgatcaatat ctacctaaaa aataacgaat taatgatgca
      361 tatgttaaag ttgttttttt gaattaaaca actcaattta ttcagttata tgttcatcgc
      421 agcaagtgaa cattaatcga gttataaaat taataggagc taaaaatata aataaagttc
      481 gatcagaatt tatttttcca gaagattttt ttaactggtt tttttatcaa ttcagtgaat
      541 gcaagcgact cttaggtgag aattattact taaaaggaat cggcggattt atggggaatg
      601 tggctgatga acaaaatatt ataaaaggaa tatctgaaca aacatcagag cttacagtaa
      661 ctaaagcatt tataagtaat ggtgaaagtt tgaaaaatgt aactgcgcga attagaaaag
      721 aaaatgaagt agacattgtt tttggaattg accatgaatg taattcagtg atatatgtaa
      781 cacaatcagc aaatttaatt ctttttgaga atggttgcaa ggacgatttt atggtggtct
      841 atttgtacga tatactaatt ccaaaacttc gttccttata tatgtcttct gaaagtattt
      901 ttgtgagtga acaaaaggga tctttctcga aaaaaatagg tattgaagta attgaatcga
      961 ttatgtgtca taatcatatt acgctagaag aattagaaat tttgataggg caaaaaaata
     1021 ataacacgaa aatacaagtt tcttagaaat cattttttat tgagatatct agataaatga
     1081 tttattcttc aatcaaccaa ctcgccaagc tgaggtaatg ttttgaggta atgataacat
     1141 acaacttttt ataccctagt aaaactctat aaacaaagac ttattaattt cgcatgaaat
     1201 acattcctgt aaaatgcaat aaaatagagt tttgtttaac caggtcttaa gaaagcccgt
     1261 aaagcttcac agttctcaaa acgttaattg cgattatcgt tcgagttcac gagttacgag
     1321 aatcgtactg ggcgaagcaa tgctgagcgc agttacctta atcctaactt ttctggcact
     1381 ttccacaatt tggaaagtgc cttttttgtg tttttagcta tgttaatagg taggattctt
     1441 ggtctataat atcattcgat ctataaacaa acgggaaaaa gataaagtat catcaaacaa
     1501 tattatttat ccgcttctga ggttgtgaca gaagtggtca gcttcaagaa ataagaagga
     1561 atttacaaaa attgcttctc atgtttgata aatattgtga cttgtgaccg aggagttggc
     1621 aggagaacac cgtttattcg gaagaaggga ttgtgaaaag tcttttgtca taacctctta
     1681 tttggtagct aattgtaaat cccaaacggc atgtattatt tttcataagt ccgtttttaa
     1741 ggattaatgt tttcttttgc tttttatatg gtctggcttc agcgagaagt tccaacgcat
     1801 atacaaggac gtctgatgtc aacgataaca acgattacca tggtggccag tccacttgga
     1861 ttagcactgt ttggtgcgct ctttgattgg cagacaactc aaccacgtct gcgagacagt
     1921 ctgatttttc ttgaaattca attggaaaat ttacactttc tttatacatt tctggctgct
     1981 ttctttatac ccgtcaacta tacttcactt atcaataaga aagaaggata agtatggatt
     2041 ttggaaatga aacggtcttg aaagcaactg gtataaccaa aaaatacggt gctgccaaag
     2101 cattagataa agtatcgatc gaaatcaaac gtggaatgat ctatggtttg attggggaga
     2161 atggagcggg taagtctacc tttatgagaa ctattatggg tttgatctcg attgatgaag
     2221 gttcgatcga attatttgga acgacggatt tgcaggcagc aagacgacgt atgggtcaat
     2281 cgattgaaac ccctgctctt tatccagaat taactgcaag agataaccta cgtatccaag
     2341 cagcgaacgg cggtgtgtcg gatagagaaa tcgaagacct gctgaaaatg atgcgattgg
     2401 aaaatacagg gaaaaagaaa gcaaagaatt tctctttagg tatgcgccag cgtttagcta
     2461 ttgcaaatgc actgatcacg aatccagaat ttttgatttt ggatgaaccg acgaatggga
     2521 tggatccagc agggatggca gaaatgcgag aaatcatcca acgtttggta aaagaacggg
     2581 gcatcaccgt cttgctttct agccacttgc tagatgaatt gtcacagatc gccactcact
     2641 acggtatttt gcatgaagga cacttgatca aagaattatc gaaagaagaa ttggcacaag
     2701 aatctcgtca atttataaaa attgacactt cggcgacgga acaagcagta acggttctcg
     2761 actctcttgg ttatagggat tattttgtcc aatctagccg tgttatccag ctttttgaag
     2821 gaattgacca agtagcagcc atcaatcaag cattggtaga ggcaaaagtt cctgtggatg
     2881 gtatccacct agttgggcaa aaactggaag attatttctt acaattaact ggaggcaatc
     2941 cacatgttta atctattaac tgctgaaaaa ataaaagtat ggaagagccg taaaatgtgg
     3001 gtctcactag gactgatggt cgtacttccg cttttttatt cttggaacga atggtatatg
     3061 catacaaaat acggcaatga attaaaccaa gcaacggata cagtcatcaa cggtgcaaca
     3121 gggattttaa tggtggaaaa aatggctgca tggattcttc tggctttcgc agcatttgcg
     3181 tgcttttata ttggggaaga atttcaaaac ggtacgattc gcaatacgtt atctttagga
     3241 cggaatcgaa tgacttatta tgtgtcgaaa cttctcgtta cgcttttgat cacaacactt
     3301 ggcgtggttt tgattacggg gctagccatg attgcttaca cacttgcttt cggatttggt
     3361 gaagttgagg gaatcaaaaa ctatggaaat tatgttctaa aagtatttcc tgtattgctt
     3421 ctgctgatat tagcgacatt atctgttcca gtggctttga cattcatcac tagaagtacc
     3481 agtgtcagtt tgcttctttc ttttctttat atcatgggaa cggccttcgt tcccggtgtt
     3541 ttcgcaaaaa tcaaaggctt ggaatttcta acggagtggt ttacggaaac gtggctcatg
     3601 tatacggact ttgcgcaaca agcaacttat tcgcaagcac cgaaaatgat tctggtcagt
     3661 ctagtaacaa tcgtggtatc atcagctcta ggaatgttta ttttccaaaa atctgacatt
     3721 aagtaggtgt tcctgctgga aactattttg attatcgaag acgatgaagc tgttcattct
     3781 ttactggagg aggttcttga acagagatac aaacttcttg atgcttattc aggtacggaa
     3841 ggaaaattat tgttaagcac atatcctgtc gatttgatcc ttctagatct catgcttcca
     3901 gggctttcag gtgaagcatt gttagcagaa atccgacaga catcgaatgt tccgatcatc
     3961 gttctttcag caaaaagcga tcaaagagat aaagtttcct tgttggcagc aggagcggat
     4021 gactatgtga cgaaaccatt tgatatcgaa gaactgttgt tacgaatcgg catccaactt
     4081 cgtcatcaga cttctgtcca agtaaaagat ctgtcgcaac gcatctatta caaggaaatc
     4141 ttactggaca aagagtcgag ggatgtaaat gtgaatggtg tggctgtcca tttgacagga
     4201 agagaattcg atctgctgaa gcttttttta gaacatccga aaaaagtatt ttcgcgagca
     4261 aatctttatg aaacagtttg gcaagaacca tattttgata gtgagaaaac agtgaatatg
     4321 catatttcca atcttcgagg gaaactagca aaggcacatg ctgtgtatat tcatacggtg
     4381 tggggagtgg ggtttagatt tgattaagtg gctgttgctt ctcgttgtta tctttatggg
     4441 aagttttatt ttcttcctgc aaagggaaat caagagatta actaagaaaa tcgccactct
     4501 ttctgaacga caagaatatg gtggtcgtct ttcagtcgaa tttcgagaaa agaatctaat
     4561 ggacctagta gatgcactga atacatgggt ggatgaaaat gaacaaaaag gacaagcatt
     4621 tcaagaaatg gaagaaaaca tcagactatc gatcgtcggc ctttctcatg atctcagaac
     4681 tcctttgact gctattaacg gttatgtaca attactggaa caggtagagg atacggagaa
     4741 acgggagaag tatttaacga tcatcaaaca atctgttgca agattactca gtatgacgga
     4801 tcagttttat gatcttgctc gaatagagac gaagcaaaaa gaaatggatc tccatacgct
     4861 ttctttttct aaagaggtgg aagaagtatt tttctctttt tatgaacaat tttctgagaa
     4921 aaatatcgag gtagcgttcg ctgaacactc gataaataca actgtattag cagatccgct
     4981 catgttgctg cgagtgctgc aaaatatcat acagaattta ttgcgttatg cagatggaca
     5041 agcaaaggtg acattttcaa aagaggattc attcattcga ttaacagtgg ccaatcctat
     5101 caaaccagga gcgcaaacct ctgttgaaaa agtcttccag cgtttttata ccgaaaatac
     5161 cagccgcaca aatacggaat ctagcggaat cgggctttac ctgtcaaaaa aattggtgga
     5221 aggaatggga ggagagatga ccgctagatt agacgggggc atcttctcca tctccgtcaa
     5281 gttgcggagg gtttaattgg ctctaattta cctgaagtga atgacaaaaa attgtatctc
     5341 gttttaaaga catcttcttt tttggaaggt gtttttattt tggtgtgatt gatacgaatt
     5401 aggtaacgaa gacagttatt tttgtacccc cttttcaaat agcgcaacag tgctatacta
     5461 aaactaacaa atcaaggaac ggagagggat atgggccaaa gagaatttat cgttttaatt
     5521 attattgcag ttgttatttt attagttgtt ttagacattt tttcacgttt gaaagtaaag
     5581 gaaacagttc gtagtaattg gggaaaaatt ccttatcaac cgagatttga taaagaagaa
     5641 agtttgaagg atgcgtggct cacggagaaa aaattccgtt catgggatag tgaaatcgac
     5701 gatcttactt ggtatgactt ggatatgttc gaagtatttg aagggatcaa cagtacctat
     5761 tctagtgtag gttcagaagc gttgtatcag cgtttacgta gctttgattt tggagaagat
     5821 tatcagttag aaaaactaat tgctttttat caggaaaatc ctcaactgag agaaagaatc
     5881 caatatcaat tcgctcgttt aggaaaaaaa gatcataatt ttgcaaaaca atatctagcg
     5941 gatggaaaaa gtaaaaagat tggacgcgta ggtttattta tttttcttgg cttgcttccg
     6001 ctggtgggtg taggtttgct tcttttaggg caagtactag gaatctatct agcaatcggt
     6061 ggcttagtat tcaatacggt ctattatcta gtgaagaaac aaacactaga aacagagctg
     6121 aatagcatga gttatttggt ttcaacgatt tcagccgcca atcagttagc aaaaatgacg
     6181 acaccacttc aaaaagagct gaaacagcaa actaaaccat taagagacat ccctaaatta
     6241 ggtttttcat ttcgaatgaa gagcgggagt gaagcagaat tgatttttga atatgtcaac
     6301 atcatgttca tgctgccatt tatctcctat catttcgttc tcactaaatt agaaaagcat
     6361 tcaactgaag ccatcaagct gtgggaattg ctgggagaat tggaagttgc agcagccgtt
     6421 ttgaattatc gcacctatat gccgatcact tgccgaccag agtttactga gggcggtgtg
     6481 acagcgaaag atatctatca tccattgtta aaagaagcgg tagttaatcc agtcaactgg
     6541 cagaagaata cattagtcac gggttccaat gcatcaggaa aatctacgta tgtcaaaagt
     6601 atagccatca gctgtattct agcacagacg atccaaacag cgattgcaga acagtttacc
     6661 ctgcaacctg gacatgtgtt gacttccatg gcaatcgaag atgatttatt tgaaggcgac
     6721 agttattttg tttcagaaat caaatcgatc aaacgtttgc tggaccaagt ggcttccaaa
     6781 gaacgctgct actgcttcgt agatgagatt ttaaaaggaa caaatacgat cgaacggatt
     6841 gcttcttccg cttcagtcgt tcggtggctt gcggagtatc catctttagc atttgtcgcc
     6901 acacatgata tcgaattgac agagatttta aagaattatt gtgagaatgt ccactttgaa
     6961 gaacaagtaa cagaaggaaa aggaatcagt tttgatttca aattaaaaga aggaccttca
     7021 aggacccgaa atgcgattgc cttgctgaaa gttttgaatt atcctaaatc catagttgaa
     7081 caggcccaaa aagaaagttt gttgttcgat gaacagcgtc agtggtatcc gtttgattaa
     7141 ctttatcaaa cgaagcatga tgcgtgggtc caaaaagacg attatcaaaa gaagtacatt
     7201 gatgccaaaa aagtggcaga attcaatggg aacgagtctg ctgtccagat gatcttggaa
     7261 gatttagcaa aagacgatac aaaataaacc acgctgtctt ccttctagca gcttttatcg
     7321 aaaaaactta gcatcagcga cgagacgcac gttccatggc aaagttctcc gtaaaacaaa
     7381 cagcgaaaac agaaattgaa aataactatt tccattttat ttatttcggt taaaataaaa
     7441 gaaaaaagct ttcgataaaa ggacggagga aagcttaaca tgaataaaaa agacaagaac
     7501 ctcagttata aggatcttct atatgtcatc gtagtagttg ctgtcggtac ttttatgatt
     7561 ttaaaagttc cagagttgtt tagatagtta ttctatcgaa gatgatcatg aactagaaaa
     7621 cacatctcct ctttgttagc aggtgtgttt ttgtgtggaa gccaagttgt tttttcatct
     7681 gctgtattcc atgatacaat taagaatcat gtctataagg agataggaaa taaatggcaa
     7741 atgcgcttga aataaataat ttgaaaaaga tctatggaag tggggtcgaa gcattgcggg
     7801 gaatcgatct ttcggtgaaa gaaggagatt tctatgcgct attagggcca aatggagctg
     7861 ggaagtctac aacgatcggg attattactt ctttagtcaa taaaacatct ggaaaagtga
     7921 atgtcttcgg ttatgatctg gacaaggatc tagttcgagc aaagcaacag attggattgg
     7981 ttccacaaga gttcaatttt aacccttttg aaacggtcca gcaaatcgtc gtgaatcaag
     8041 caggctacta cggtgtctct cgcaaagaag cgatgaaacg cagcgaaaaa tatttgaagc
     8101 agtccaatct ttgggaaaag agaaatgtcc gtgcacgtat gctttccggg ggaatgaagc
     8161 gaagactgat gattgctagg gcattgatgc atgaaccacg tttgctgatc ttggatgaac
     8221 caacagcagg agttgacatc gagttgagac gcgagatgtg ggagtttcta cgagaattga
     8281 acgaaaatgg tacaacgatc attttgacga cgcattatct agaagaagct gaaatgcttt
     8341 gtcgcaacat cggaatcatc caatcgggtg aactgatcga gaatacaagt atgaaagagc
     8401 ttctgtcaaa actgcaattt gaaacattta tctttgattt agcgccatat gataccaaac
     8461 ctgtgattga aggatatgct tgtacatttg aagatgagca gacaatcgct gtcgaagttg
     8521 aacgaaatca aggggtgaac ggaatcttcg atcagcttac aagacaagga atcaaagttc
     8581 tttctatgag gaataagtcg aatcgtttag aagaactgtt cttgaaaatc acagaagaaa
     8641 ataatcatac ggaggagaaa aatgtttaat ctttatttta ctgcattaaa aagtttagct
     8701 atcaaggaaa cgaaccgcta tttgcgtata tgggtccaaa cactggttcc gccagtgatc
     8761 acgacatcct tgtattttgt tattttcgga aaaatgatcg gtgggcggat cggtgacatg
     8821 ggtggttttt catatatgga attcatcgtt cctggtttga ttatgatgtc tgctatcact
     8881 agttcgtatg ccaatgtttc ttcgtccttc ttctctcaaa aatttcagaa aaatatcgaa
     8941 gaaatcttgg tagcacctgt tcctacgcat gtgattattt ggggctttgt gattggtggt
     9001 gtgggtcgca gtattctcgt cggctctctc gtaacgatca tttccttgtt ctttgtacca
     9061 cttcatgtgt attcatggtt catggtcatc attacattat tgatgacggc aatcttgttt
     9121 tcattagcag gactgctcaa cgggattttt gctcagtctt atgatgatgt atcgatcgtg
     9181 cctacatttg tcttgcagcc gttaacttat ctaggtggcg ttttctatgc catctctatg
     9241 ttaccgccct tttggcaagc tgtttcaaaa gtcaatccga tcgtttatat gatctccggt
     9301 ttccgttacg gattccttgg aacgatcgat gtgccagtag caacatcgat gatcgtactt
     9361 gtgttattca tcgttgtttt gtacgccgtt acttggtatt tgatcaataa aggaagagga
     9421 ttgagaagtt agaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
     9481 acgggaaaat agttcctcat attttgttgt tttttgagcg aataccgagg gtcagctgat
     9541 tgaacaccgt ttagtg
//
LOCUS       DACJGB010000094         9531 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.096, whole genome shotgun sequence.
ACCESSION   DACJGB010000094 DACJGB010000000
VERSION     DACJGB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.096"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..779
                     /locus_tag="IYI34_002623"
     CDS             <1..779
                     /locus_tag="IYI34_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR0560453.1"
                     /translation="RSVASAMSFIMMDGQSHALLDIVENRRLPYLECYFSRFSLATRE
                     AVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTARLKGTSH
                     SQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVDRLLSACPNLRQGY
                     QLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLPQIQNALHYSYSNG
                     PLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            899..1099
                     /locus_tag="IYI34_002624"
     CDS             899..1099
                     /locus_tag="IYI34_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAR0560454.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1175..1372
                     /locus_tag="IYI34_002625"
     CDS             1175..1372
                     /locus_tag="IYI34_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560455.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1669..3216)
                     /locus_tag="IYI34_002626"
     CDS             complement(1669..3216)
                     /locus_tag="IYI34_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAR0560456.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3300..4103)
                     /locus_tag="IYI34_002627"
     CDS             complement(3300..4103)
                     /locus_tag="IYI34_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAR0560457.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4106..4888)
                     /gene="phnE"
                     /locus_tag="IYI34_002628"
     CDS             complement(4106..4888)
                     /gene="phnE"
                     /locus_tag="IYI34_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAR0560458.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4885..5664)
                     /gene="phnE"
                     /locus_tag="IYI34_002629"
     CDS             complement(4885..5664)
                     /gene="phnE"
                     /locus_tag="IYI34_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAR0560459.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5636..6427)
                     /gene="phnC"
                     /locus_tag="IYI34_002630"
     CDS             complement(5636..6427)
                     /gene="phnC"
                     /locus_tag="IYI34_002630"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAR0560460.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6488..7417)
                     /locus_tag="IYI34_002631"
     CDS             complement(6488..7417)
                     /locus_tag="IYI34_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAR0560461.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7612..8295
                     /locus_tag="IYI34_002632"
     CDS             7612..8295
                     /locus_tag="IYI34_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HAR0560462.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8456..9499
                     /locus_tag="IYI34_002633"
     CDS             8456..9499
                     /locus_tag="IYI34_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0560463.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2921 a         1949 c         1892 g         2769 t
ORIGIN      
        1 ttcgaagtgt tgccagtgca atgagtttta tcatgatgga cggacaaagc catgcattat
       61 tggatattgt cgaaaatcga cgcttgcctt atttagaatg ctatttttct cggttttcat
      121 tagcgactcg tgaagccgtt caatggattg ttattgatat gtatgcgcct tatgtttcat
      181 tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga ccgtttccac atcgtccaac
      241 atattggcag aaccttcttg aatcatcgag tgaaagaaac cacggcacgg cttaaaggaa
      301 cttcccactc tcaacgaggg ttagggaaaa aattgaaacg ctattggaaa ctcctacaaa
      361 aagaagaagc aaagttaaac tacgaaaaaa gggtttggcg tgccagtttt aaagattatt
      421 tgactgaaag tgagattgtc gatcgactgt tatctgcttg tcccaacctg cgccaaggct
      481 atcagttata ccaagacatt ctttatgcgg taaagaaaag agatcagtca ttatttgagg
      541 actgtttaaa cagagaagtc agcggtcttc ctgaaaccta tacaaccact ttacgaacgt
      601 ttaaaaagtt tctgccacag atccaaaatg cgcttcacta cagctattca aatggaccat
      661 tggaatgttt aaacaaccac atcaaagtat taaaacgcaa tgcgtacggc tttcgcagct
      721 tttacaattt caaactacgg atcatgattc gtcatggcaa aacattttta accaaataaa
      781 cagaacagaa agagagtttc ttctttctgt tctgtccaaa ttattctaag atttctcttt
      841 accaacatca tttgacaaag agcctgtatt tattactgtc gaggacaatg gcatcggcat
      901 gattgaagag gaacaaaaaa acatttggga tcgctattat aaagccgatc cgtcaagtaa
      961 aaatacaaag ataggcgagt caggtcttgg attatcaatc gtgaaggaac tagtgcgcct
     1021 gcaccacggt tcaattgaag tgaccagccg aaaaaatgaa ggaacgacct tcctgataca
     1081 atttccaaaa gaaaaataaa attaccagta aaaaaccgtg gagagatagc tgctattgat
     1141 ttttgctatg tcttttcttt gagaggtatc cggtatggtg gagtgtctga aaaataaaaa
     1201 aatggaggcc aattttgtga aagctgttat ttctggaagt attgttttcc aagaagaata
     1261 tcgagatatg gaaaaagatt tcagtaaaaa aggcattgaa ttaattgatt atcctcgctc
     1321 atgtccgatc ctagacaaag agtatccaga aatcttgacg actttttttt aaatatcgca
     1381 gcagcggatt tattttttgt atttaatcaa gatcggaaag gggtattagg ctatattggt
     1441 gccgcaactt tttcagaaat gaagtattgt ctgatgcaaa atcttattca tggaaaaagg
     1501 atcaaaatag tcttatttaa ggaaccatca gaggaaaatt ttttgttttg atgaggtaga
     1561 tcgctggcta aagaatggct ggatggaaat cagacagcct aacaatttgg cataacaaag
     1621 gtgtgggcaa aaaactgctc acaccttttt gaatttttta tttatatact acaaaatcac
     1681 ttgaaaatta tgattcactt ttgcttcgat gcgtgggtga atattcaaat agtcggcaat
     1741 taattcggtc atgtcgattt ggatttctcg gacaattttc tccgcaccaa acatcgcata
     1801 atttcctccg ccgattgccc gatattgatt tgtcaccact tccagcattt gttgcggaag
     1861 aacggggtga cctttaaaac gaagttcggt aatccgttgt ccaacaggct gtttcatatc
     1921 aatagtgtac tgaatccctt catacatgtc ataattgtag tactggggct taggctgaat
     1981 aaaggctgga ttgaatacga tttggttatt ttctacagct aaataatttg cggtctgctc
     2041 caacgccgct ctcaaatctg caccatttat ttgaatcact gccagagtat tcggataaat
     2101 atagttggtg atgacgtccc gcatcgtgat cgtctcacca aagccttttc cttcattatt
     2161 gaataacgca gttccagaaa tctctgagcc gctggcaaat aattgtacac gattaataaa
     2221 ctctacatag ggatgttcct caagacgagc ctgcatcgga tcattaatac gcatatttcc
     2281 gttcacttta cctaagggtt gatccaacca tttctctagt ttttcttcta ccggtacgat
     2341 cgctgcaaga atttctggat caggctctga tttttcaact gagtgtaaag caacctggtg
     2401 atctgtaaca accacttgcc cctctttttt ctcgacggta agacgaattt caccgatgaa
     2461 ctcgccacga tagcctggtt gaacaacggg aactccatga accgtttcag caattttccg
     2521 gtgctgatga ccggtgaaaa gggcgtcaat gccttcaacc tgctgcaata gctcgtagcc
     2581 ttcattctct ccagttagcg cttctgtgtc ctcaccggtt aacaagtctt tttcaaatcc
     2641 gccgtggtaa ctaacgatta ccaagtctgc ttgtttcctt aatagaggaa catattcttt
     2701 tgctgtttcg acgatactct taaacgtaat tcctgctaag gtttctggct tttcccagtg
     2761 aggaatgtat tgtgtcgtta atcccaagat agcaattttt acacccacct tttcgataat
     2821 cttataggcg tttccaaagt atggttggtc atttttgttc accatattgg ctcctaatat
     2881 aggcgcttga taattcttaa tcgcttcctc gagataatcc acaccatagt taaactcatg
     2941 atttcccaac acttgaacat catagctcat ttggttaatc aagtctgtaa gctctttggc
     3001 tgaaccatat tctttagcaa gataataact caaaggagac ccttggatga aatcaccatt
     3061 ttcgatcgta attaccggtc catctgcttt tgcccgtaat tccttcagtt tcgacgatac
     3121 tttagccgtt cctaaggcca gatcggttct gccggaatag tctgttggtg aaacatagcc
     3181 atgcatatca cttgtctcta aaaacgtaat gttcattctg cgtgctcctt tagctgactc
     3241 aaaatagaaa aaccgaagcg tattcagctg ttcggtctct ttttttgatt tataatgcct
     3301 taatccttca tttgaagtgt ttttaaaatg ttgcctggaa taaagttttc tgttgtctgg
     3361 cgaacaatcc ttccttgatc gagatgataa aggttatacc catgacgttc ttcaaagcgt
     3421 acgaagccat cgccttcatc ttcaccaaca aaggacatac tcgatacagt aaaatagggg
     3481 atcccattaa tttcatcagt aaatgtgtga tgggtatgac cgtttaggat acagcgaata
     3541 tcgtaaggtg cgattagctt caagaaacgt tcggtaccag gccattttgg tatactgctt
     3601 tggtgagaca gtagatgatg atgggtgacc aagaaaatcg gttggtcttt ttcttgctct
     3661 aaggcttttt tcagccattg aaattgctga tcgtccacta taccatctgc gtatccatag
     3721 cttgaattat caaaggaaat cactgtaaac tcaggatatt tgactatctg gttgtacggg
     3781 gcagaagagg cggcttcatc gcaccagcct acccaaaaat gttctttgat atcgtgattg
     3841 cctagagtaa caagaatcgt cgtttctcct agcgcatttt ccagccaagt cttcaagtat
     3901 cgataatcgt ctatttcgcc atcttcggtt aaatctccgc tgataatcac aaggtccaag
     3961 ggtgtttgct gcaggagatg atgaatgcat tgctccagag ggataagggg attctgcatc
     4021 gtagccagca ttcccttata gccttcttca caagcctcat actctctgcg aaagtgtata
     4081 tctgatatat gcaatatatt cattcttatc tcaatctctt tcttataatt ccggttattt
     4141 gatctaaaat aaacacggtt acgactacgc caatcaaaat aatactcact ttgctccaag
     4201 accgagtttg aatcgcgaat atcaatggag caccgattcc gccagcgcca actaagccta
     4261 aggtagccgc actacgaacc gctatttcaa aatgatttaa tgctaaagaa caataaatcg
     4321 gcatcaaata ggggatgcga acaaagaaaa tcgtttgcca aaaatttgct cctaccgcat
     4381 gcatttcttc aacaagtcgc tcatccatcg cttccatttc ttctgtaaaa agtttgccaa
     4441 gcattccgat ctgatggacg ccaatcgcca taacaccagc aaaggggcca ggtccgacga
     4501 ctttcacaaa aataattgca aaaactagtt caggaaaagc tcgtaaaaca ttacagataa
     4561 gcttgccaat ttttgttacc catggatgtg cttgccagag gttaaccgca ctgatcaagg
     4621 taatgggaat cgctaatacc gtagcgatca ttgtccctaa acaggcgatt ccaatggtta
     4681 ataatagcaa actaatcaaa tcctcgccgc tgccatcata tagaaactgc caatcaggct
     4741 gacctaaccc tctcaaaacg tccattccca tggaaggaga gaaggtgctt aatccagagt
     4801 aatccagagt agcggccgac aaaataacga gaatcactaa agcaactaat gataaaaggg
     4861 ttcttctgtt catttcacga agcatcataa cagggacctc ctgacacgtt cacttaattt
     4921 atcaacgatc gcgacagtta ctaggatcgt tagaataatg attgaaactc gttcataatt
     4981 caaaagcgca agtgaggaat ttagaataac gccaatacca ccagctccga catatccaag
     5041 gacagtagaa gcacgaacat tcacctcaaa cgcgtaaaaa gcatagctcg caaactgact
     5101 caaaacttgt ggagcgatcg accaaatagc gatttgcatt ttatttgctc caacagccgc
     5161 agcggcttca atcgggccaa agtcaatggt ttcaattgct tgaaacatta attgtgacac
     5221 cataccaaaa gtaaagacag cgatagtcaa cacgccagtc gcttcaccaa ttccaaacac
     5281 agcaacaaaa agcgcggcta aaagcagggt agggatggtc cgtactaaac ctaaaagaaa
     5341 acgaaagatg gtagaaagaa gacggttttc tgtcactaca gtggtcgcta aaaaactaac
     5401 agggatcgct aaaattaagc caatgaccgt tccaagcaaa gacatttgaa gtgttttcac
     5461 cataggctcg actaatttag gtaaatagct aaagtctgga tgaatgaagc gtgataaaaa
     5521 gacagtcatt tgattggagt tttgaaaaac ctgagaaaaa tcagctgacg tgatccaggc
     5581 acttccttga aaacaaacaa ttaagaacag aagaataagc aggtgtttat accatttaaa
     5641 gtgaacggtt tctttcaagc tcatcgattt cgcctgcctt ttcttcaaag attgcattgc
     5701 cataaatttg ctctaagcgt tcatcggtta agtcgtctgg acgaccatca aagacaacct
     5761 gagcagcttt taacgcgagc acacgcgtag aaaactcacg agccagaggg actgaatgca
     5821 gattgacaat gactgtttta tccatcgttt gattgatatt ttttaggtcc atcataacct
     5881 tttgagtcgt gattggatct aatgatgcaa cgggttcatc cgctaaaata atgggggctt
     5941 gctgcacgag tgctcgcgca atggatactc gctgttgttg cccaccactg agctcatcac
     6001 tgcgtgtctg caatttatca gacaaaccaa ctttttccag tgcttccttt gtctgctggt
     6061 agtcttcctt cgtaaaaaga cctaaaatac ttttccaagt ggagtaatag cccaaacgtc
     6121 cagatagcac atttttctga accgtactac gctttaccag attgaagctt tgcgagatta
     6181 gaccaatctg gcggcgaatc tgtcgcagct cttttttatt cgcttttgta atagaggttc
     6241 cattaatgcg aatatctcct ttggtggttt ctgccaagcg attgatactg cgcagcagag
     6301 tgctctttcc agcaccactc aaccctatga tggaaacaaa ttctccatct tcaattgtta
     6361 agtcaatatc cttcaagcca acaacgccat tgggatagac cttcgaaaca ttgtcaaaaa
     6421 taatcattat ccttctccat ttttttaagt gtaagcaaaa aaaagcttgc tgacacttac
     6481 tgatgtttta ttctgctgct tttttcgtgt agtctctgat cgtgtcatag gcagaatcag
     6541 cagcttcttc atagccctga tgcccccaca aagccattgc ttctttgcca tctttatcat
     6601 ccttcatatt taagaaggtc tctttaattt tcgcttttaa atcttttgac atgtcaggct
     6661 gtacagcgat tgcgtcattc ggaatatcgc cttttgtcag gtacaggact tttaaatcct
     6721 taaataaatc attatctggg aaattcgaag caaaaacatt tcgcgcgcct tcaaaaacaa
     6781 acgccgcatc catttgcttg ttcaacactg cggtaatctc actcggaata tcattcacag
     6841 tggtcatctt tgtatctgca gtaggatcaa tcccagcatc tttcaattca gcgacaggat
     6901 agatgtagcc gctagcagag ttaggactta aggttgcgat attttttcct tttaaatcag
     6961 ttaacttatt caatccgcta tcggcacgaa ccaatacttc tcctttaaag gtgttcgtaa
     7021 gtttgtcctt taatggcaag ccagtttctt gatcataatc gccgagctga gaagtcagga
     7081 ttgcatctgc agctccagca tcctttgctt gaacataggc tgcaggcggc ataattccga
     7141 catcgacttg accagaagac atcgcttcga cgatcgttga ataatcagta gctaacgtca
     7201 cttctacctc acgatctaac tttttcgata aatacttagc aaatggtttt gcttttgcct
     7261 ccatactacc atcattgttt gttggaacga attgaacaac taatttttct ttgctctctt
     7321 tttctgagct gctacttgta ttcccagaac cgcaggccgc caaagtggtc aataacatga
     7381 ctccgatgaa cccttttgct aactgtttga actgcatttt tctcctcctg ttggatataa
     7441 tttctaatac gtctaaatca tagcagggaa tcaaaatcaa tgaagtgacg cacgtcctat
     7501 tttacacaac ttttacatac aatttacaaa caattataaa taattattcg tattgagccg
     7561 aaaaacaatc taagctataa taaagaaaaa cttcttagag gtgacgattt catgaaaaaa
     7621 atccatgata cgaagcaatt aactgcttac gttgagaagc acaacctgca ggattatatg
     7681 gattcagata tattggcaat ggcttcttta cttttttttg aaaaggaaga gcacctcatt
     7741 caaacgggaa aaacctcaga ttatttgtat tttttagtgg atgggacggt aatggtgtac
     7801 tcgtattctt ctgatacaca aaatatttgc atcgattacg caaagcccgc tacaccactc
     7861 ggcgaagcct cttcattgtg ggaattatcg ccgaaaagca gcgttaaagc agtgactaag
     7921 tgcatctgta tcagcattcc gctaaatcaa tatcgaacac agcttcaaaa agatgttcgc
     7981 tttttgcaga atatttgcca gttactaagt tatcgtttaa attccgggat aaatttggcg
     8041 aactcactga cagaacctgt tgaaactcgt ttagcaaagt ttattttgac ccatcacgaa
     8101 aacgaacgtt tctcatttcg gttaaccacc tgcgcctcaa tcttaaatgt cagttatcga
     8161 catcttttac gaacaatcac gaattttcgt gaggagaata ttatcgaaaa gaaaaagaat
     8221 tattacttga ttcatgatat cgaagcttta gaggacttag ctgagaatat aagtagtcca
     8281 gtaaaagcag aataacagct ataaaaaaac agcagtcaga agtgattgct ggttttttga
     8341 tagtggagaa attgggaaac ggaaaatctt cgttgaagag tagtgttcta tatattggta
     8401 tgctatactt ttaaaagttc aatgtgtcaa gacacataaa aaggggagaa aaattttgaa
     8461 gaaaatactt gtttcattgt tgtttttggt gtcattagta acgttagtcg gctgtagtca
     8521 gtctcagggg gagaagcaaa gtgaaaaaga gaaaagtaaa atcgctttgg ttcttggatt
     8581 tggcggggtt gatgaccgct ccttcaatca agctggctgg gaaggattag aggcttgggg
     8641 aaaagagaaa aagctgacag caaaaaaaga ttttacgtat atagaaacga acaatccaga
     8701 ggataacagt accaatatcg atcaggcaat cagaaacggc tttaccacca ttattagctt
     8761 gagctacttg caagcagagg aactggaaaa tgctgctaaa gataatccaa agttgaaatt
     8821 tgccttggta gatggtgaga ttactgggaa aaagaatatc tcatcagcaa cctttaaaga
     8881 tcaagaaagt gcgtatttag caggactagc tgccgcatat acgacaaaaa gtgatcaagt
     8941 aggctttatt ggtggaatgg aagggcccgt gatccaacgt ttccaaagcg gatttgaagc
     9001 tggcgttaag gcaggggcca cagcactgaa caaggaaatt actgttaacg tacaatatgc
     9061 tgcatcctat gattcgctag ataaaggaaa agctattgct gcttctatgt acaaagaggg
     9121 agtcgatatt ctttatcagg cggcaggaag tactggagct ggggtgttcc aagaagcaaa
     9181 agcccgcaat gaaaacgact atagcagaaa agtatgggtg attggtgtag atcgcgatca
     9241 agcggatgaa ggggattata aaatggagaa cggcgagaca gggaatttca cgttgacttc
     9301 tacaaagaaa aatgtcggtg cagaagtcaa agaatttgca gaaacttctc ataacgataa
     9361 atttcctggc ggcaagcatt tgacctttgg tttgaaagaa gaaggggtag atctgacaaa
     9421 aggacaatta tcagaggaag cctatcaggc agtttcagac gcaaaagaga aaatcatcag
     9481 tggtgatttc agactgtaga caaagtccat tttatggatg atgtctacag t
//
LOCUS       DACJGB010000095         9417 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.098, whole genome shotgun sequence.
ACCESSION   DACJGB010000095 DACJGB010000000
VERSION     DACJGB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.098"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..368)
                     /locus_tag="IYI34_002634"
     CDS             complement(<1..368)
                     /locus_tag="IYI34_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR0560464.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
     gene            complement(365..607)
                     /locus_tag="IYI34_002635"
     CDS             complement(365..607)
                     /locus_tag="IYI34_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAR0560465.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1153..1683)
                     /locus_tag="IYI34_002636"
     CDS             complement(1153..1683)
                     /locus_tag="IYI34_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR0560466.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1865..2521)
                     /locus_tag="IYI34_002637"
     CDS             complement(1865..2521)
                     /locus_tag="IYI34_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAR0560467.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2648..3121)
                     /gene="dfrF"
                     /locus_tag="IYI34_002638"
     CDS             complement(2648..3121)
                     /gene="dfrF"
                     /locus_tag="IYI34_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAR0560468.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3540..3758)
                     /locus_tag="IYI34_002639"
                     /pseudo
     CDS             complement(3540..3758)
                     /locus_tag="IYI34_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3789..5003)
                     /locus_tag="IYI34_002640"
     CDS             complement(3789..5003)
                     /locus_tag="IYI34_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAR0560469.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(5344..5586)
                     /locus_tag="IYI34_002641"
     CDS             complement(5344..5586)
                     /locus_tag="IYI34_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR0560470.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(5618..6175)
                     /locus_tag="IYI34_002642"
     CDS             complement(5618..6175)
                     /locus_tag="IYI34_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR0560471.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(6188..6376)
                     /locus_tag="IYI34_002643"
     CDS             complement(6188..6376)
                     /locus_tag="IYI34_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAR0560472.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(6389..6955)
                     /gene="amaP"
                     /locus_tag="IYI34_002644"
     CDS             complement(6389..6955)
                     /gene="amaP"
                     /locus_tag="IYI34_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAR0560473.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            8185..8658
                     /locus_tag="IYI34_002645"
     CDS             8185..8658
                     /locus_tag="IYI34_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAR0560474.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            8667..8894
                     /locus_tag="IYI34_002646"
     CDS             8667..8894
                     /locus_tag="IYI34_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560475.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT         2879 a         1847 c         1368 g         3323 t
ORIGIN      
        1 atatacgatg tactaggaat aatttcttca taaatcttgc cataagtttc atatatttta
       61 tccatagcac caatatgctc cacaaccaaa tatttataag acggtatttc tttatacaga
      121 aagccctctg gggtaatggt tctttgggga atagaacaga aataataaag ttttccattt
      181 cttctttcca taaaagcata ttttacccaa tttcctgatt gtgaaagata cgcttttttc
      241 aaactactgt taaactttct ccaaaactgt gtactaatct gaatattctt tttttgataa
      301 ttagtcagtt ctacttcctg cccaataact gcaaatgcgt ttaattcctt tatagaaaaa
      361 tttatcattt taccttgtga gcagtgataa cggtataact gtacgcattt accgttataa
      421 tacaattatc aatcgttata taccagtttt ttccttttct tgtgatttcc gcattagaaa
      481 ggctaatttt ttctttgcac cattcaatta cattttctac attcaaagat aagttctttt
      541 taattctaat aacgccaagt tcagttgtgt gaagtcttcc taagttttcc agtaattcat
      601 tttccatatt ttgatacctc atttttcagc aaatttattt gtatgtttta taccccaatt
      661 ataacattct catatcagca ctacaataaa aataattaag caccaataat cttattgaac
      721 atctccaacc gtaaatcttt cacaccatca gtgataaaca ctgagctgac agcaattacg
      781 acaactacca taattgcctg cacttaaatt tctgcaaaag aaaacgacaa tcaatttctt
      841 attttttgaa atcaactgtc tttctcatat ttactattca attttcatag tgatataggt
      901 acaatttata tcactactgt aaaaatgggg ttttgcctta taaatacagc gttttccgct
      961 tgttagtgta tagtgtttct tgttttttgt gccccccttg ttagataacg ggggctttga
     1021 acggctcgca agctcgccga accgccgaag cgaaccgccc ccttgcaagc atggcggaaa
     1081 aatcttaagg attttcccgc caacgtggtc ggatcgctcc gtcggtttaa cagcttttct
     1141 gtttctctct agttatctac gcatataaat caaatcggtt attccgttat aagtctgtgt
     1201 tttacaaagc cttaacttta tttccctgtc tatgttgccc aaaagacgaa ccccatttcc
     1261 taacagagta ggaataacag aaatgtaata tttatcaatc atatttttgc ttaccaactg
     1321 ctgaacaaga tttgctccgc cacaaatcca aatatccttt cctgcttctt gttttagccg
     1381 ttccaataat aaaacaggat tttcacttgt aaaatgaatt tgttgggaag aattgcactc
     1441 tttatgtgtt aatacataag ttgtaaattt attatatacc cattcttgcg gagaaagttc
     1501 agtaacaacc tgatgagaag tattccaccc cattaagaca gtatctatat ctttggtaaa
     1561 ttctgtataa gtgtcgatgt tttcattgtc atttccatga ccatttagcc aagctactcc
     1621 accattaccg tccgcaatat atccgtcaag gctcatggca ataaataaaa ctacttttct
     1681 cattttttct cttttataaa tacaaacagg aatttatttc tcttttttac tcataaggga
     1741 acaactgatt gctacaaata aacccattaa aatccatgga aatgctgctc ttataaaatc
     1801 actaaccata ctttattcct ccttaaattc caatttcctt agttctacaa actgaaattt
     1861 ttgcctatga atgtatcttg ttctttatac gcaaacaggc ttctgtgtgc ataatccaat
     1921 gtcttgaaaa cggcatttga attagtccac aaatatcttt attacaccaa taatcaatca
     1981 gccaaattgc tttttcattg tcgtttacta cattcaacga ttctaagtat ccttttcttt
     2041 ctttcggtat ttttcttttc aattcatcat aagataaccg ttcaagcatt ttctcagtgc
     2101 tttcccatac ttcgaaaata tataaatata attcttccag attaagctgt tttgaaaagt
     2161 ctgcaatctg ctgtttcatg agttcatttc ctgttgtaat gataggtgaa ttgatacgct
     2221 cttgataatt gcctgcaaaa aatacttgtt catcttcatt tatcactgta tgcacaacta
     2281 tatcttcaat gcggaaaata tgccaaatag aataagcaat cgtcttacta tgataaccat
     2341 cagcgtttat gaaggggatt gagttaaaat cttctctgca taattcttct ttgaaagata
     2401 ctaaggtttg tattaactga cttcgtaaag taagtaaagt atcaataccc ctcttataag
     2461 tatctttctt ttttatttgt gcctgcattg ttttattaag ttcagaccat tccttattca
     2521 tattcaatcc tctctcaaat tctgatttta tcgccccata caaactggaa tttgtcttac
     2581 aacaatttat atatgcataa aaagcaatat atttatgaaa acaccctaaa tccaaaattt
     2641 actcaattca ttttttcctt acataaaatg ttctcgtata tttcacttcc tcaccaagtg
     2701 tttcccctat caaaacttca aaatcattga tatcaaattc tggaaagaat gtatctccat
     2761 cttcaatatt taaatctact tctgtgatat acattttatc tactatttgc aaagcttcct
     2821 taaatagtcc atatccacca gatatgtata catctcgtcc tttagccaat aataatgcat
     2881 cttctaatga tttaactgaa actaaattat ctccttgata ctctgttgtg gtggaaacaa
     2941 caatattcat tctattaggc aacggatgac cgatttcttc ataagacttt cgccccataa
     3001 taaccacatt acccgttgtt aactctctaa attgcttttg ttctcccttt attttccatg
     3061 gtatattacc attcttgcct ataacattat tctttgacct cgcaacaatc aaacctatca
     3121 tctatttcat cctccataat atgatgtcgt acctgtgatt ccatcaagtg caccacatct
     3181 taacaaactc aaaattatct cgaactaacc agctcgacaa attccttttt tgtcttcatc
     3241 tgcattatat catattgtta taagcattgc catttctttt ctgcttttga aaagttactt
     3301 atcagcctat gttttgtaaa gggtgatatt cagcatttct cacgcaacaa gcccttgaca
     3361 aaccatagac taataagttt taaggtaatc aagcagaaat gaaatagtct gctgtttcac
     3421 ggcaacaaaa aagagatacc aacacactta tgtagtatct cttagtctag tagcaatatt
     3481 cttttgtttg caagcaggta aacatgggct gtttcttaat atctttttgt ttgggaaaca
     3541 cctttttccc atcctttcac cgttgccatc tctgctgcgc acaatgccac agggataaac
     3601 catagcaaac cgcctaataa caagaaaaat actaagctga agcctgaggt cgcgaatgtg
     3661 ggatattcat ataatgaaac gaccattgag atggtcatag ccaaaaagcc aaagaacgtt
     3721 aatttttttg caggaatttt cgttttgctt tgttgcatga atcttcctct tttctatttg
     3781 aatttgtttc aattgagttt gttgccaaag aagaaaaagt ccaatccttc ttcttgttct
     3841 tcttttgtag tagtgatcgt tgtaaagtgc atcgccgatt ttaaaatcac tgttgtcagc
     3901 acataggcat acacggcagt aatgatgaca ccaatcactt gaatccctaa gaagtaccag
     3961 ctgcttggat tcgttagtaa atggctgtta gatgcaaaga tccctatcag aatcgtacct
     4021 gtgaatccgc ccatgccatg aacaccccac acatcaagtg catcatccca ttgtttcttt
     4081 ttacgaaaat caacagcagc atgacagacg atacccgcaa tgatcccaat cagcatggca
     4141 ctttttgctt ctacataacc ggcacaaggc gtaatggctg ccaatccagc aacggatcct
     4201 gttagtacgt cgacaaaatc aaagccattt ccttttactt ttgcaatgat cagccaagta
     4261 atcatcgcaa atgcaagagc gatgaatgtt gaaccaaatg cattgactgc ttgttcatct
     4321 gctcgaagtg caccaccgga attaaaaccg aaccagccaa accaaagcag tcctgtaccg
     4381 atagcagctg ccataagact gctatgctga cttctttctc ctaaaggaat ctttctttga
     4441 ccaagtgtga ggatacatgc taaagagccg aatccagctg tcgtgtggat gactgctcca
     4501 ccagcgaaat ctctaaatcc catctgatcc aaaaatccgc caccccatat ccagtggcaa
     4561 acagggaaat aaatcagcag attccaaaaa ataacaagta taatatagcc ttttaaattc
     4621 aatctccctg caaaagctcc tgtcatcaaa ggaacggtaa tcacgataaa catcagttgg
     4681 taaagaaaga acattaaaaa tggaatcgtt gcaccatgaa acatgttggg gaagcttgta
     4741 acatgtctca tcaaaaagaa atctgccggg tttccaataa caccgccgat atcccgtcca
     4801 aaggccaatc cgaatccacc aaatacccac ataatcgttg ttatcccaac ggagagaaaa
     4861 gattgagaca tgatcgttag tatattattc ttccgtgcta atccgccgta aaaaaaagct
     4921 agaccaggag tcatcaggca aaccattgct gtacataaaa tcatgaatgc tgtatctcct
     4981 gtattgatag gtaatgtatt catttttttc gcctcctttc tacgattacg agtataaaag
     5041 aaggtgtagt tgtttttttc ttctgttttt ttgaattaat acttgcattt ataaaagaca
     5101 gacaattaat aaaagcttga tgtgttgtgc tctttttcgt ttttcacaca aataaagaca
     5161 aaaaataaac ggtcttcttt ttatagaaaa ccgtttattt tcgtcttttt ttatttttaa
     5221 agatattttt tcataaggaa taaagaatac ttcatataaa atatataaaa aagaaccgca
     5281 gcgagtgtcg ctacggttct caaatttgtt cttcatttca acataagttt ttcttcaatt
     5341 tgcttataat ttacgtgcaa agaatgaaac gactaatact aaaatgattg caccgatcaa
     5401 tgatggaatg atagccattc ctgcaattgt tggtccccaa gttcctagta aagcttgtcc
     5461 aattgctgaa cctactaaac cagcaatgat gtttgcgatg atccccattg atgatccttt
     5521 attcgtgatt gctcctgcaa ttgctccaat gataccgccg acgattaatg cccaaataaa
     5581 tcccatttta aattcctcct aactgacttc ttacttctta ttgtacacgt ggttcgttat
     5641 tttctttgac tttttgtgca cctttgccta cagcagattt tgctttatct gtttggcgtg
     5701 aagcaaattg tcctgtggat tttgctgctt ctgttacttt gtcttgtact gtttcttgat
     5761 ctttttggta ttcttcttgt gttttgatgt ctgcaacatt cacattcact tcgataacat
     5821 ctaaatgtgt catattgttt acttcagtac tgatcaattc tttgatttgg ttgtaaatat
     5881 cttcgatgtc tttaccgtat tctacgatca catctagatc cacagctact tgtttcttac
     5941 ctacttctgt attgatacct gctgttacgt tatctgtgtt gactaatttt tcagccatat
     6001 ttgagaaaaa tccgccatct aatgtcaata gaccatctac atctttcaat gcataaccga
     6061 tgattttttg gatgactttg tcttcataag tcaattcacc attcatcatg cggttttctc
     6121 ctttttcatt tgccaaaact gatccatttg ttgtattcat gtttttgttt tccatctttg
     6181 tttcctccta tgatttaaaa aattgatcaa ctattcctga ctgtttcaaa taaaaaccga
     6241 ttcctgcgcc aattgctgtc atgattatga tcaacaatgt cttgaaaaat ccaatcgtta
     6301 accataaaac agctagcagt agtccaagac ccccaaagat aatagggagc ttgtatgctg
     6361 aaaatagttc tttcattatt tggccctcct attccactcg agagtgcttg tagtctcttg
     6421 tatcttcttg ttcttcataa ccgctgtatg tgactgccac tttgaccgtt tcttttgttc
     6481 cgagtacttt agttacttct tcttcgatat cttgcatcaa tacacctgtt ttccctacta
     6541 atgaagaagt tcgtttcaag tctccgttga catggatatg gattcgattt ttcgttgaac
     6601 gtatccgtac tttaggtgaa tgaatgaact cttcttcgtg taaatgggaa cgtaccaacc
     6661 cttcgatcgc ttttttgtcg attgttagtt tgccatcttc ccgtttcata acgaaagttc
     6721 ctcttgactt tggatagaaa agaacgacta gcaagatgat aatagcaaga acggcaaata
     6781 ctacagctgc ccagaaaata tattgttgca tgtagtaatt cgttaaagta aagaagcgaa
     6841 atcgttccaa ttgttcaggc ataatatacg gcgcattgat caacgcaatc gctagaaata
     6901 ctgaaagtag cagtaaactc atgataacac cgattatttt taacccctta ttcattttcc
     6961 acctcctata aacttatttt taccctgtaa ataactctac cgacaatcac agtatagata
     7021 gcgaacagtt agcatagcat cagtaaataa agcgctacca aacattgctg gatcataacg
     7081 gcgttttatt gccagtctga tactgaaata acaattgctt acttattctc tatttacttt
     7141 tgattgctgg ctatacttaa aggatattat tcaagattaa aaattgcaaa gattatgctc
     7201 agttattatt acaagattct atttatttat taaatttttt cggagaatta aaaaatacaa
     7261 taaataactt tagttacttt aatgcgttct tactttattt ttatattatc atttttctta
     7321 ttcacttctt tggtttatac tattcttata aataaaaacg aggtatgtcc tatgatgaaa
     7381 agattgtact attcattgat cataacgatt ggctatttga ttgtatcaaa tctaggaaat
     7441 atggtatttg gtatcagtaa agaattcagt tggactacta cattatggga atctctattc
     7501 tttttcatct tcgtattttt gttacaaaat tatcgaaaaa aataaacatg tgccatctat
     7561 ctgttggcac atgttttagg atttatggta ctttttattc ggcaatccat tttatttttc
     7621 tacttcttct taatacctct aacacttttg atttataaac taatccaatg catctacttg
     7681 cgcttattaa gctatttcag ctagatagta ccaccccatg cgtcaatgct tgattttcct
     7741 tttgtataac attgtataag caataaagga caaataagtg gtgattacac caaaccattg
     7801 aataagaaaa attctgatac gtaaataaac atgaacttga aaaaattaat gaatcggtta
     7861 gctactacgt agttgtcttt tttctcatac agttctgtta acttacactt ctcttcgcta
     7921 gataataaaa aatcctccaa ctgtattgtc caatataatc ccaggcatct aaactagcat
     7981 tatagaagag acacacatag aatctaaaat aactggtttt aaatgtttct tacaatcaaa
     8041 aaaagctgtt aaatgcactc cacaccttca tactctatca ttttttataa acgatatatt
     8101 tttattgttt ttcgataaaa aatagttgtt ttacaataat tattctgcta tattttagct
     8161 atcaaagata aaagagggat aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt
     8221 ttaggtataa gtgccgtatt tattttgttc ggcgtacttt tttatactca gatcgctaca
     8281 agcgaaaaag gattcaattt tgattggcag accacattat ttgtttctgt catttacttt
     8341 gcccttctcc tagggatcgt tattgcttat ttcttattga aactattgaa aaaaatcgaa
     8401 gataaccaag ctttttccac tcaaagctta aacatactaa aaaaaatcga acgaaccatt
     8461 ttgtttattg gtttcgcctt ttttggctta cttcctaaat ttatgaaagt agcggattta
     8521 gacgatgctc caggtttgat gttggtagga atcgctgtcg tattcattcc ttttgcagta
     8581 tacacattaa caagcgtgct ggaaaaactg cttgaacatg cgattttact taagacagat
     8641 catgatttga cagtgtgagg tgcgacatgg caattatcgt taatttagat gtagtattag
     8701 ctcaaaaaaa aatgagtgtg actgaattga gtgagaaagt gggcattacg atggcaaaca
     8761 tctctattct aaaaaatgga aaagcaaaag ccatccgttt ttccactctc gaaaaaatct
     8821 gccaagtttt ggagtgtcaa ccaggtgatt tgttgaccta taaaaatgac tcgaatacta
     8881 taccagaaga ataaaataaa gtagaacaaa cagtaaataa aaaataatct gaatgataga
     8941 tgtgtgagac ttttagaagt tatttacgtc tattattcag attttttatt tcgattgaat
     9001 accgttttat ctataaataa gatatgtact gactcataca aagggggcct tttcttctcc
     9061 atgtactatc acttttcctg atacaactgg tttcctgtta cgttgtagtt ggatctgctt
     9121 ttagtataat actttattca ttccaccaga ctgatagcag tttgagcagt ctaaataatc
     9181 atgtagtatt acaggccatt ctgtttcata ccttgaagtg cttaaaactt cagctaatgg
     9241 cccattgtgt taactcttac tccattcttt tttagtaagc tcccatatat actgtaaagc
     9301 cttctatata ttatgtcatg ttgcttttta acatttacta tgtagttttg ataaaatgat
     9361 ttgaataaca gtgatggaca attctttttt atttttaaca ttattttgat aataatg
//
LOCUS       DACJGB010000096         8498 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.100, whole genome shotgun sequence.
ACCESSION   DACJGB010000096 DACJGB010000000
VERSION     DACJGB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.100"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="IYI34_002647"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="IYI34_002647"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAR0560476.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="IYI34_002648"
     CDS             complement(1098..2201)
                     /locus_tag="IYI34_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAR0560477.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /locus_tag="IYI34_002649"
     CDS             complement(2291..3226)
                     /locus_tag="IYI34_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAR0560478.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="IYI34_002650"
     CDS             3528..5462
                     /locus_tag="IYI34_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAR0560479.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="IYI34_002651"
     CDS             5919..6335
                     /locus_tag="IYI34_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAR0560480.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="IYI34_002652"
     CDS             6349..6915
                     /locus_tag="IYI34_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAR0560481.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="IYI34_002653"
     CDS             6938..7924
                     /locus_tag="IYI34_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAR0560482.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8498)
                     /gene="scm"
                     /locus_tag="IYI34_002654"
     CDS             complement(8079..>8498)
                     /gene="scm"
                     /locus_tag="IYI34_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAR0560483.1"
                     /translation="STDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2508 a         1637 c         1722 g         2631 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt tacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacaagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgct
//
LOCUS       DACJGB010000097         8493 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.101, whole genome shotgun sequence.
ACCESSION   DACJGB010000097 DACJGB010000000
VERSION     DACJGB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.101"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..173)
                     /locus_tag="IYI34_002655"
     CDS             complement(<1..173)
                     /locus_tag="IYI34_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560484.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IYI34_002656"
     CDS             complement(593..1714)
                     /locus_tag="IYI34_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAR0560485.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IYI34_002657"
     CDS             2042..3451
                     /locus_tag="IYI34_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAR0560486.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IYI34_002658"
     CDS             3448..4176
                     /locus_tag="IYI34_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR0560487.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IYI34_002659"
     CDS             complement(4304..5215)
                     /locus_tag="IYI34_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR0560488.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IYI34_002660"
     CDS             complement(5232..6239)
                     /locus_tag="IYI34_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAR0560489.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IYI34_002661"
     CDS             complement(6236..8365)
                     /locus_tag="IYI34_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAR0560490.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IYI34_002662"
     CDS             complement(8370..8477)
                     /locus_tag="IYI34_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560491.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACJGB010000098         8424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.102, whole genome shotgun sequence.
ACCESSION   DACJGB010000098 DACJGB010000000
VERSION     DACJGB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.102"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            471..2108
                     /locus_tag="IYI34_002663"
     CDS             471..2108
                     /locus_tag="IYI34_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAR0560492.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSMGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IYI34_002664"
     CDS             complement(2253..2993)
                     /gene="truA"
                     /locus_tag="IYI34_002664"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAR0560493.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3184..3819)
                     /locus_tag="IYI34_002665"
     CDS             complement(3184..3819)
                     /locus_tag="IYI34_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAR0560494.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IYI34_002666"
     CDS             complement(3966..5207)
                     /gene="sstT"
                     /locus_tag="IYI34_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAR0560495.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5405..6401
                     /locus_tag="IYI34_002667"
                     /pseudo
     CDS             5405..6401
                     /locus_tag="IYI34_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6417..>8424
                     /locus_tag="IYI34_002668"
     CDS             6417..>8424
                     /locus_tag="IYI34_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAR0560496.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKILTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQK"
BASE COUNT         2785 a         1547 c         1293 g         2799 t
ORIGIN      
        1 aactaatcag aatcgtacaa atatttaata accaaatata tagaacataa cttaatatta
       61 agttatttga aattccataa aaaacgactg caggaattat tgtaagaaaa ggataaaaat
      121 agttcagtcc atctccataa ttgttaaatg ttgtatagtt gattggccct tcaaaaatag
      181 aagataaccc tttgattcgt gaaagatgaa aagataaatc acctgttgta taaatttctt
      241 gagcatttct cataaaattt actgaatata aagtactaag taagataaga actactaaaa
      301 gaaatgcttt tttacttatt ttcatagatt tgttgttctc cttttttaat tctattttca
      361 gtatactaga attgtctttt tttaataagt tttgcgttaa atttttcata gcactagtat
      421 gatatactag aaaagaatca atttttatta cttttaaata ggagaaaaaa atgaaaaaaa
      481 acaaaaacca aataatagat atcattttta tgttatttct aagttttgct tacgtcatcc
      541 ctctattcat gtctatggga atctatcata gtgtcaatca ggatacttac tttcatttat
      601 ctcgaatcat aggactagat aatgtatgga gttctccagt aaacttcaat aacttcgatc
      661 atcatggaac aatgatgaat attttttatc catggcttac tctttatcca gcttttttat
      721 tttataaaat gatgggaaat cttgttttaa gttataatat ctactatttt tttatcactt
      781 ttttaacaat ggtcgtttct tatttttcta tgaaacaaat aaaaaacaat cgttatattt
      841 cgctgttatt ttcgattatt tatacatttt ccgcctatcg tgccattgac atattccatc
      901 gtgcttcatt gggagaagct gtcgctttaa catttttgcc tttgatacta atggggtgtt
      961 acgaaattta tattagagat taccaaaaat ggtactggtt gtctattggc atgacgctag
     1021 tagtgtatac tcatttactt tctgtcgcta tggtttctgt atttattgga ggaacgttgt
     1081 ttctttcttt ttatttttgg gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta
     1141 ccgtcctcac ctttttttta agtgcgggat tcttaattcc ttttattcaa caatctagag
     1201 cacaagaatt gaaagtccca ctgggaaaag aattaagtgg tatggctcct agtgatatgc
     1261 tgacacacat tttaaataat aactataaca actatactat tggattattc ttatttctcg
     1321 gattgattgg tgcttttatt tttataaaaa aattaactgc tgatgattta tttattttct
     1381 ttcttggtgt atttgttctt ttttgttcaa ccaatttatt tccttggcaa ctttttaacc
     1441 atacaccagt taagagtcta caatttgtct ggcggttaaa tggtttttct tctttattta
     1501 tagcctatac gatgagtata gtgatctatt atattttttc tactaaaaat aattcatgga
     1561 aaataatggt ctttacgttt cttgcactaa tattgcatct ctcaggagta agcaattcta
     1621 tccatgctaa caaagattca ttaacactta tagcacctga agacgctgtt gctatagctg
     1681 aaaattacaa tcatactgat tatgcaaaca aagagtcaat ctatcatcct gatatgatta
     1741 ataatgatca atatttacta ggtaaccagg aaataaatcc aacaactcat tttatgagta
     1801 acaaacttac aattgaatta gataattcga ataataaaaa tactgtcttg acgacaccta
     1861 tttatcgtta taaagggcaa gtggcttcta ttaatggcaa actagtacaa acaaaattat
     1921 ctaagtttgg cacaactgaa ttaactattc ctccaggtat aaataaagtc gttattactt
     1981 atcagtacac aaaactagca attgcttcaa gatatttatc tatcgttact ttgattttat
     2041 ttttacttta tcgatttact ttttctaaac aaaaacaacc gagaagagaa gtacaacatt
     2101 ctcactagat ttttatttaa gaaagacttt ttagctgtac acataaacag gctaaaaagt
     2161 ctttttttat atttctttaa tttgcttcat acattattta acatcgatat ttatactgtg
     2221 aaacatgcgt ttcatccatt atcaaagtat ttttatcgat aataaacttc atccaaaaac
     2281 agaccttgtg cagggacggt ttctcctgca tgttgccgca ctttgctttc aaatatttca
     2341 tcgattgttt ctatcggcat ggcacctgtg ccgatttcta gtaatgtccc catgataatc
     2401 cgcaccattt tatataagaa tccgtctccg acaaaagtaa aatgaagcat ttctcccttt
     2461 cgttcgatag ataattcatc aatcgtacgc actgtggatt tttttgattt tttcaatgca
     2521 gaaaaaccaa tgaaatcatg tgtacccact aatttttcgc aagctgcatt cagcttgtcc
     2581 atatccaact cttgagggaa ataaaagctg tggtttcgct caaatgctga aggaatcacg
     2641 ttgttccaaa cataatagct gtattttttg cccaccgcat tatatcttga atggaagcgc
     2701 tcaggcattt cttcgattct tttgatgaca atatcccgcg gaaggtaacg attcataaag
     2761 tcgatcattt ctgccagtcc aatttctgtg tttgttttaa aatttgctac ttgtcctcgc
     2821 gcatgggtgc cagcatctgt tcttcctgat ccaataattt ctatctcttc atttgtcatc
     2881 tgatgcaata ctttttcgat ttttccttga atggttttat cgtcatctcc aagtctttgc
     2941 catcctgaat atctttttcc gtcatattcg attgtcaact tgatgtttct catattttag
     3001 tggctcctct gtcttccatt tggtcttctc tctacctttt taagattgta gcacaaaata
     3061 tagctccttt cttctctaaa acagaagcta tacaagaaaa ctgtggaaat atatatttca
     3121 gaaaaacaaa aaacagccac cggatgaagt atccgctgac tgttcaattt gacggctcaa
     3181 gttttactgt ttgagatgca agatactcaa ccaggcaatg ggcacataat taaagaaaaa
     3241 actgattctt tggcaaaaac ctattttatt cagtaatggc caagaattta tagaaggaat
     3301 tcttgccagt ccataaatcg ccgcaaaaag caaactgatc acagacagtc caaggtataa
     3361 gtggcttacg tccatttgcc cattttttcg atagatcaag aaaagaagaa ctggaaacaa
     3421 taaaaaacca ctgtaaccta aacctgatcc gatattatgg acccatgtcg agacattcca
     3481 cgaagattca tgggtattga tactgaacaa acctgtaaag atacaatcac ctataccata
     3541 gaacgcgatg gctgctgtaa ctagggtagc taacacacga gatgtactta caaatgcagc
     3601 atacaatgca ggcaatgaca ggatgaaaaa gatccctgaa accacagacc aaataagaaa
     3661 cgcttttctt acaggactgg aaacctctcc aaatacactg atcacgctct tcatttgatc
     3721 taattcagga taatacaatc ctaatacgta cggagtcaaa aaatcactca gcacgcctaa
     3781 taataaaaag aaaaaaccaa atttacgtaa aaaatccatg atacgcctcc aataacaatt
     3841 acctatgctt ttaattgtta ttataagcga ctttatcttg atttaacaga aacctctatt
     3901 tacacgaaca tctatataac tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct
     3961 aacgttcaac gttcacttaa tccatctaaa caatctttca atttgatttt ttccccactt
     4021 cttttacgcg cgcctagctc tcctattgca gtaaacaaca gatcacttga cgaatttaag
     4081 gcagtttcca ttgaatcctg gataacaccg ataataaatc caataccgac gatttgcatc
     4141 gcgacatcat tagagatacc aaacaaacta catgctaatg gaatcaataa cagtgaaccg
     4201 cccgcaacac ctgaagctcc acaagcagct aaagcagaca aaatacataa aaggactttc
     4261 aaaacaaaag gtgcttcaat ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg
     4321 gtaatcgcgg caccgcccat attgatcgta gcacctaacg ggatcgatat ggaataagac
     4381 tcttttgtca atttcaatct ttcagccaac atcatgttta tcgggatatt tgcggcagaa
     4441 cttcgggtaa agaaagcggg aagtgcgctt tctttcaaac aaaagaaaat caatggatag
     4501 ggattttctt taataaacaa aaacgtgagg aatgggtaca caactaatgc gacaaacagc
     4561 atcgtaccga tcaaaacaag taaaagctgc agatactctg ccaatccagc tattccagtc
     4621 gtagcaattg attgataaac caatcctaaa atccctatcg gagcaaatgc aataatcact
     4681 tgtacgactt gtgacaacgc tatagatatc tgatccacca actcttttgt cgattctgaa
     4741 cgtgctcgaa aagctagtcc caacaataca gcccagaata agacgccaag gtaattcgca
     4801 tcaataagag attgaacagg attttgtgtg acattcgtca acatagtaga taaaacactt
     4861 tccaaacttt gcggtgcttt ttcagatact gtttcttgta aagtaatagg aacagtaaaa
     4921 attgaactta caataactgc tacaagtgca gaaagcaatg ttcccactaa atataagatc
     4981 aaaataggct tcacgaatgt cttattccca attttatgct tggcaattga cgccatcgtc
     5041 aagaaaaaaa ccagaatagg tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat
     5101 attgaaataa atgtccatgt ggggaaaaag ataccaaaca atattccgac taaaatacca
     5161 acaacaatac gttgaactaa cgacatatcc aaaaacttct ctaacaaatg accacccttt
     5221 cacaatttta acaatcattg tatcatttta tgcaaagaaa ctaaaggtat aattttctaa
     5281 aaattctaac aatacaaaca accatttctt gctaattttg tactttttct gtctacacgt
     5341 tgttttttga gatattctaa gttaggatgt tattaatggg caacttatca ggaaacgttt
     5401 tcttatgttt ttgtccaatt tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga
     5461 gagttatggc ttcctgacaa tctttgtcgc ttgtataatg atggttttac agcgtatatt
     5521 gatttcagca gtcactattc ctacatatgc aacaaaccct ggtcgattct acatctatac
     5581 atttttacaa acttccgttg ttattggtgc aaatttgatc ccactttaca tgggttacca
     5641 atatcaaaaa atcgcccgtt taaaagtttt acattattta agtcgttttg cctttatatt
     5701 cctaatagcc acactagttt gcaatatctt ttttttacgt acaagctggc agcttgaata
     5761 ttcgtgatta ttggcttatc ctaacaccta tcagtcagaa ttactttact tatgctgttt
     5821 cttgtacttt gcttttttgt agtattcctt atacagttgg atttttaaat aaattatcga
     5881 aaacaacaat taaatatcta ctattatttt taacaattgg attagttggc tgctctactc
     5941 tatttaataa agacccgttt gcattacaga atggacagag tattttatgg attttttatt
     6001 tatttttaat gggttatggt ttgaaagaat ggaactggaa agaaaaggtc cgctataagc
     6061 ttcctcaagt atttatcagc ttgattgttt tattcggttt gattattttg atgacacaaa
     6121 tttcactcat catcagagga aatacagata cagcgctgag gttttctaaa ccatattctc
     6181 tcttcgcaat gtattatact ttctcttcgt ttttattttt agaaatgcta tctgataaat
     6241 taggtataaa aggaagagga aattttcttg ctgtattttt gattgtcact caagttatat
     6301 gtaatttccc tttagtttct tatcgcgtat ctacatttat gaaacgtgaa tttccaaact
     6361 cagggaaggc atggctgata aatatcgggc agttttttat ttgctattta ttagcagtgt
     6421 ccctcctagt tatcttttta ttttatatac aaaaaatccg tttcattcat cgtttcatcg
     6481 aatacttcgc ttttgattcg attgaacaat taactcagcg ttggaaaaaa agaattcatt
     6541 ggctttttgt tcataaaagt tattttttag ttgcagcatt tttttactgc tttaccttcg
     6601 tgcagatttt cgttttagaa ccaaaagaag gttggaaaga aactatccag gttgccttaa
     6661 aaatatttac tcagcgacaa gcacccatga tcctgacaat catcattatt atgggatttt
     6721 tcttcttact attgttgatt tccaatcgtt tttggtacgc cttgactgct accttgatca
     6781 ttaacttact gctcactatt tcgacagtaa tcaaaatgga aatgcgggaa gagcctgtct
     6841 ttccttcaga tttaaaaata ctgaccggat tgtcagaact attatcgatg gttagcccag
     6901 tcttattaat cgtgggcgga ttgatccttc tcctattatt gattactagt attattgtac
     6961 aacgtagact acaaaaacag tattccttga agattcattg gaaacgtcga tttatcagta
     7021 tcgctgtatt attaggatgt ttttcgggtg tctttttcat taatcacaaa aattcgcctt
     7081 ctttcttgct gtttaattta tttaaagtaa acaagacttt ctttaaccag caaggcgccg
     7141 taaaagataa cggaccagtc attcaattcc taaacaatat cgacattaaa atcatggaag
     7201 aacctgctgg ctattcgaaa gaaaaaatta atggaataat gaaaaagtac aacacaaagg
     7261 cagatgaaat caaccaaaca agaagtgact ggttaaacaa tcagacgatc gttctcaatt
     7321 taagtgaaag cttcagtgat cctgcacgtg tccctaatct gacagtacct acagacccta
     7381 ttccttacat caaaagcatt ataacagaag gaacaggagg attgatgtta tccgtcggtt
     7441 atggtggcgg aacagctaac atggaatggg aaggactgac aggcttgagt atcagtaacc
     7501 tgtctgcttc gttagttaca ccttataccc agctagtgga gcgtcagaaa ataagtccaa
     7561 atattaccga tctattcgat gaaaaaatag ccatccatcc ttttacagct gctctttatc
     7621 gcagaaaaca agtgtttgat aaatttggtt ttgacaggtt ctattattta gacagtccgg
     7681 ataaattaac ttatacggat aaaatagaac aaagcccacg catatcggat gattcagcat
     7741 acaaagaaac cttgaaagct ttacaaagca atacagaaac gtcacaattc atccaattat
     7801 ccactatgca aaaccatatg ccttatgatg attactatag tgaattgaac tacactgctg
     7861 aaggaactgc tgtcattcct aatagaagaa aagaattaca aacttatatg caaggccttc
     7921 actataccga tacagcagta aaaaaattca tccaagaaat cgacaaaatc gataaaccga
     7981 ttacctttgt tttttatggt gaccatttac ccgcaatcta tagtgggaat gattcgaaaa
     8041 aatatggatt agaacagcac cagacagatt atttcattta taacaacaag tatagtcgtg
     8101 aacaagcagc tacgttaaat aaagaagttg ttgctcctaa taactttggt gctatggctc
     8161 ttgagcatgc gaatatcaaa gtaactcctt actatgcgtt gttgacagaa gtatctaatc
     8221 atctacctgc attaacaatt gatcctacag aaagcttgtc taatcgtttt aatggaaaac
     8281 aggtctttgt tacagaggat agtcaaatta tccaagaaaa aaatttaact gagaaacaaa
     8341 aagaaattct agaagattat cgtctgatac aatatgatct tgttgcaggt aaccagtaca
     8401 gtgcaacatg ggcgacacaa aaaa
//
LOCUS       DACJGB010000099         7978 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.103, whole genome shotgun sequence.
ACCESSION   DACJGB010000099 DACJGB010000000
VERSION     DACJGB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.103"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IYI34_002669"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IYI34_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAR0560497.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1227..2330
                     /locus_tag="IYI34_002670"
     CDS             1227..2330
                     /locus_tag="IYI34_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR0560498.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2523..3590)
                     /gene="rlmN"
                     /locus_tag="IYI34_002671"
     CDS             complement(2523..3590)
                     /gene="rlmN"
                     /locus_tag="IYI34_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAR0560499.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3820..6267)
                     /locus_tag="IYI34_002672"
     CDS             complement(3820..6267)
                     /locus_tag="IYI34_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAR0560500.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6456..7892)
                     /locus_tag="IYI34_002673"
     CDS             complement(6456..7892)
                     /locus_tag="IYI34_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR0560501.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2213 a         1736 c         1358 g         2671 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa aaaaaaccaa ttatgaattg acaaataaaa cctgttttca tataataatc
      961 ttaattttta gaatttttag ataattacaa aaaagcaaag ggaatcggta gaaatactaa
     1021 gaggaacaaa aattcattat tgtcgttttc aatgtttttg tacttgttcg tttttaggat
     1081 acttattgat gatttcaagg tgttcatgcc accttattca tcacttatcc tgtcttaggt
     1141 tcacctctac tccatctaca gccgcagtca gatcgatcac tctttaaaat tttaacccgt
     1201 ttcaacgaag aaagaaggaa atgaatatga gtcaaagtgt tttcttacca aattatacca
     1261 ttggaacaga tgcgtatgaa aaagcaaatg gaatcgtcaa agaatatggg acaaaagtag
     1321 tgctgattgg agggaagact gcactttcaa aggcagaaaa caaaataaaa gtagctttga
     1381 cacaggaaat agaggttgtc gccagtctct ggtacggggg taattcaact tatggaaata
     1441 ttgaacagtt gatccagcat gaaagtgtcc aaaaagcaga cgtcctgttt gcagtcggtg
     1501 gtggccgtgc ttgcgatact gtaaaagtca ttggtgaaaa actagctaaa ccagtcttca
     1561 cctttcccac tattggttcg aactgttcgc ccgtcactgc ggtatgtgtc atctataacg
     1621 aagaaggcgt aatggacgga ctcttctttc cttcaaaacc acctgtacac accttcattg
     1681 atacagcaat tattgccgaa gcacctgata aatacttatg ggcaggtatt ggcgatgctt
     1741 tatccaaaga agtcgaatct actttttctg ctcgtggcga tgatctaggc tacacggata
     1801 gattaggaat ccaaatcgga aaaatgtgca ttgatcgtct cttgacttat ggtacgcaag
     1861 cattgaaaga tagtgccgac gatcgtccct catatgcaat tgaacaagtc gtttcggata
     1921 tcattgttac taccggattg gtttctgttt tagttaacaa taactataat tctgctatgg
     1981 cacactcatt ctattacggt acgactgttc tagaaaaaat ggagggatac cttcacggag
     2041 catctgtcag ttatggtgta ctgaccttat tgatgatgga tcagcagttc gatttgtttg
     2101 ataaaatcta tgtgtttatg aaaaagaacc aacttccagt atcgttaacc gacatgaatc
     2161 tcaatgtcac gaccgattta gaagcaatct tagataaagc catggcaaca aatgatatta
     2221 aacatgtgcc ttatgaaatc acccgagaaa aatttaaaca ggccattctt gacgtagaaa
     2281 cttatcatgc tacacaagaa agccagcaag gatataagaa aatcagttaa ataattgcta
     2341 aaacccccat caagcaggct gtcatcaaat tgaggacatt tctttgcttg atgagggttt
     2401 ttcttgtcaa aaaaagcttt atagctttcg ctatagaatc ttttctctta tttaagttga
     2461 acagttcctt aattaagaaa actgctcctt ttatggtaat tgaaaaaacg agtaggatac
     2521 gattatttgg caactgtttt tttcatttgc ttgcttcgca attgtccgca agctgcatca
     2581 atatctgtac catgttcttt acgtatcaca cagtttacgc cgtttttctt caatacatca
     2641 taaaatctca atacatcagc ttttggactg cgagaatatt gatcatgttc actgactgga
     2701 ttatatggaa tcaagttgac atatgctaat tttttcttat cttttaatag atctgctaat
     2761 tgttgcgcat gctctggtct atcatttact tgattcaaca tgatatattc aaacgtgatt
     2821 cgacggtttg ttttctctaa atactcatcc acagcttcca tcaatttctc aattgggaaa
     2881 cttcggttga tccgcatgat cgatgttcgc acctcattat tcggcgcgtg caaggaaata
     2941 gccagattga cttgcaagcc gttattagcg aattccttga ttttgtgtgc taatccacta
     3001 gtggaaactg taatgtgccg agcaccaatt gccaatcctt tggcatcatt gatgatatgc
     3061 aaaaagtcca ttacattgtc gtaattatca aatggctcac cgatccccat tacaacgaca
     3121 tgtgaaacgc gttcgccaag attgcgctca tcaaaataat gttggaccat catgatctgt
     3181 gcaacaattt ctccagcagt cagatcacgg ttctttttga gcagcccgct tgcacaaaac
     3241 gtacagccga tattacatcc tacttgtgtc gtcacacaaa ctgacaagcc atattcttgt
     3301 ctcatcagta cagtctcaat catgttttta tcagggagtt caaacaaata ttttactgta
     3361 ccatccgaag cttcttggac gaccatctgt tttagaggat tgatgataaa atgctccgat
     3421 aatttttcga tcaattgttt cgatagattc gtcatttctg tgaaattact tacacgtttt
     3481 tgatacaacc attcccaaac ttgggcagct cggaattttt tctcgccgtt ttctataaac
     3541 caattgatta attcttcatt cgtcaagcca taaatggact gtttttccaa agtattttcc
     3601 ctctttttct caaagtttcg ctacatacta tacatgattc atttccttaa tgcaatactt
     3661 cacaaataaa tcgaatagaa ctttaatctc acaaaaacta aaaagacaag acatcatacc
     3721 gtattagaaa agatggaaaa aaaccatttt ttcttttggt atcatcatgc cttgtctttt
     3781 tattggttat tcgtttttca acccttcatc acacaagttt tacatgtcat tatcgtccgg
     3841 caacgtttct gatgtttctt cctgctcaat gaatactttc cgaggcttgc ttccttctga
     3901 cgggccaaca actccatgag cttccagttc atctactaaa cgtgctgctc ggttgtaacc
     3961 gatccgaaat cttctttgga gaagggaaat acttgccgtt tgcatttcaa cgacaagtgc
     4021 ttttgcttct tcaaacagct catcttgtgg ctgttctgga gcacctgtcg tttcaacttc
     4081 atctgtcggc atcattttct cttcataatg agcttcttgc tgatctgtaa caaattgaac
     4141 gacccgttct acttcgtggt cagaaatgaa tgctccttgc acacggatcg gtttattctc
     4201 acccattggc aagaatagca tatctcctcg ccctaagagc ttttctgccc cattagaatc
     4261 aatgatcgtc cgagaatctg taccgcttga taccgcaaat gccattctgg aaggtacatt
     4321 tgctttgatg attcctgtga taacatccac acttggacgt tgagtagcta ggatcatatg
     4381 aatccccgct gcccgagcca tttgcgctaa acgaataatc gcatcttcaa cttcattgct
     4441 ggctaccatc atcaaatcag ctaattcatc tacgatcact acgatgaatg gtaaggttgg
     4501 atgtttcgtt ccgttttcca agtttttctg ttgaacaaat tcattgtaac cagaaatatt
     4561 tcttactccg gttgccgcga atttttcata tcgttcttcc atttcttgta ctactttttg
     4621 taaggcttgc gctgcttttc tagggttcgt cacgaccggc gtcaacagat gaggtatgcc
     4681 gttgtacata ttcaattcaa ccattttcgg atcgatcatc atcagtttta cttcatgggg
     4741 cttagcacgc attaatatac ttgtgataat cccattaatt gctactgact tcccactacc
     4801 agtcgaacca gccacgagta agtgaggcat cttcgaaaga tcagccgttt gtacccgtcc
     4861 agaaacatca cgtcctaaag gtacttctag taatttatct gggtggtttg gttgtgcttc
     4921 gatgatttct ctaaaggaaa ccatactgat ctttccattt ggtacttcaa taccaatcag
     4981 tgatttacct ggaatcggtg cttccatccg gacatctttc gccgctaaag caagcgcgat
     5041 atcatctgtt aagttcacaa ttttacttac ttttacccct acagccggct ggacttcgaa
     5101 ttttgtgacg gatggtccta agctggcttt gacgaccttg gcatcgacac cgaaactttg
     5161 gaaagtttgt tctaatacgc cgatattttt ttcaattttt ttatactcat cactttgatc
     5221 cacagttggt atagaatcaa gcaaatcaac tgtcggtaat tcatagtctt gattttcctg
     5281 ttctgcttca atctcaaatt ctaatgaggt accgtcatcc tctggcaact catctgaaaa
     5341 accttctgct gtattggtct gttttggtgt atctggtact ggtttctcta gattcgcagg
     5401 atggatattt tcttgaaaac tgtcgattgg aacaaaactt agctgctccg gctctgtatt
     5461 ttctggttcc tgccattcat cagcagtatt ttccctttgc tggctcaccg cttttttctt
     5521 ctcatattct gcagcttctt tttctgcagc ttcttttgca gctaagcgcc ttgcttcttt
     5581 cgcttctgcg cgctgtttca ttctctcttc ctttgccgct tgctttcgag cttgcttggc
     5641 aggatcacct tccaataatt cctgtactcg ttctccaatt gattggatac catttaacag
     5701 ctgatagcct tccagattac tgaacaggaa tgctcctcct gccagtaata gcactgctgc
     5761 taaataactt ccaggctgtg caatcaagaa gtaagtaaaa tgataaagga ttgctccaat
     5821 catcccacca ccaacgttat tgtctacttg actttgtttt atatcactgg ctaacaaatc
     5881 ccatgtcgtc cccatgatat ttggttcccc agtatggagt ttgccaaata aatgggcatg
     5941 taacaataat aagataccga aataaaagag tatgccgcca aaccagcgtc ggtttttacc
     6001 aattgaaaac tctgtattct taatgacaat ccaaaatcct aatacggcta ataaaagaca
     6061 gaggatttgg taagtatttc ctgcaatgat ccgaaagccg tttgcaatca atattcctaa
     6121 aaaacctaat tttaaaaagc cgaagacagc aaataaaatc aaggcaaacc caacaccaat
     6181 cgtcagcatt tgttcctgct gctgtttttg ctttttcgtt tttttcgtct ttttcctttt
     6241 tttagtaggc cgtttctttt gtgccattta ttatccctca tttcatcaag tttcattata
     6301 caaaaaaaag taagggaact aaagttttgt ttgaaagaac tgacgctttc tttcattttt
     6361 cagcttattt tacagttttt cttaatttct aaatgatttt atcaagaaaa ggcacggcca
     6421 tatccagccg taccttcttg ttttaagtat gtgaactaaa ggttctctcc attttcttca
     6481 atcagttttt ggtaccagta ataactgtct ttcttatatc gttccaaaga tccttcttgt
     6541 gtttcgtctt gatcaacata tacaaatcca tatcgctttt gataaccgtt cagccagcta
     6601 agcaaatctg tataagacca ggtgcaatag cctaggactt ctgctccatc cgaaattgct
     6661 tctttgatgg ctttgacgtg gctttgtaag taattgattc gatagtcgtc atggatttgt
     6721 ttatcttctg tcaacttgtc atactctcct aagccatttt ccgtaatcag taccggcatg
     6781 cggtaacggc ttgtgatacg acgcagtccg atccttaatc cttctgggtc aatctcccag
     6841 tcccaatttg ttcgttcgac aaatggattc tcaacttttt tatacattcc aggaactcca
     6901 gtttcttctg atgaaccttt ttttcctgtc gtattcattt ttccagcacc aactccgttt
     6961 aatggattga atgcattcgt cgccgtttga tagtaattga tcccaagaaa atctggttta
     7021 gcagattcta gcaattctgc atcgccagct tcaatcgttg gtgcccatcc tttttcttct
     7081 aagaacctca tggcagcaat cggatatttt ccccacatat agacatccat ccaataattg
     7141 gccatcaggt cttctgcatt ttcagcagcc agaacatttt caggattact gtcaattgga
     7201 taatttggcg tatacgcaaa gcttggtccg atttttccag gcatttctaa ctcgtgaaat
     7261 ttattgatga cagatgcatt tgctaaatta gcaatatggt taacttcata cattcgtttc
     7321 gggtctgtca cacctggcgg atgtgctgct aacaaataac ctaatgaagt gaagatattt
     7381 tgttcattca aactaatcca gtaatggact ttcccgcgga atgcctcaaa taatgtttct
     7441 gcataatgag tgaaatcttc aatgatctga cgtgattccc aaccattata ttcatcttgc
     7501 aatgcttgtg gaagatccca atgatataaa gtcaacatag gttcaatatc gttttcaatc
     7561 aattcgtcga tcaatctctc ataaaaagcg agcccagctt gattgacttc tcctcgtcca
     7621 ttaggaaaaa tccgtgtcca agctacagag aagcggtaag ctttcagacc ttgttctttc
     7681 attaaagcaa tatcttcttt ataacgatga tagtggtcta ctgccaaatc accgttcgtt
     7741 cctttaaatg tctttccagg tatccgtaca aatcgatccc agactgattc tcctttccca
     7801 tcttcttgcc aagctccttc tacttgatac gctgcagaag cagagcccca cagaaaatct
     7861 tttggaaatg aggtaagttc tttgtgttcc attcatctct tcctttccta taaccttcaa
     7921 tcgttcctct tttagaataa cacgaaaccc cttccacaac aaaataaaat tatgtaaa
//
LOCUS       DACJGB010000100         7918 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.104, whole genome shotgun sequence.
ACCESSION   DACJGB010000100 DACJGB010000000
VERSION     DACJGB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.104"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..669)
                     /locus_tag="IYI34_002674"
     CDS             complement(<1..669)
                     /locus_tag="IYI34_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0560502.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRN"
     gene            complement(666..1607)
                     /locus_tag="IYI34_002675"
     CDS             complement(666..1607)
                     /locus_tag="IYI34_002675"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAR0560503.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(1784..2371)
                     /locus_tag="IYI34_002676"
     CDS             complement(1784..2371)
                     /locus_tag="IYI34_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAR0560504.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(2592..3905)
                     /gene="obgE"
                     /locus_tag="IYI34_002677"
     CDS             complement(2592..3905)
                     /gene="obgE"
                     /locus_tag="IYI34_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAR0560505.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            4193..5203
                     /gene="gap"
                     /locus_tag="IYI34_002678"
     CDS             4193..5203
                     /gene="gap"
                     /locus_tag="IYI34_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAR0560506.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(5288..6223)
                     /locus_tag="IYI34_002679"
     CDS             complement(5288..6223)
                     /locus_tag="IYI34_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAR0560507.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            6409..7376
                     /locus_tag="IYI34_002680"
                     /pseudo
     CDS             6409..7376
                     /locus_tag="IYI34_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            7699..7815
                     /locus_tag="IYI34_002681"
     CDS             7699..7815
                     /locus_tag="IYI34_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAR0560508.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT         2344 a         1695 c         1351 g         2528 t
ORIGIN      
        1 attgcggacg atcatttttg ccaacttggc aggttctaaa acgatttgtc cgactttttc
       61 caaatagctt ccgcttggat cagccttatc gaagaaattt gtctcgattg gacctggatt
      121 cactgtcgta acagctacgc caagtggttt caattctaat cgcaaagcgt tggagaagcc
      181 aaggactgca aatttcgtgg cagaataaac cgttgatttt gcagttgcca tttttccagc
      241 catagaggcg atattaatga tatgaccttg tcttctttct gccatatcga tcgcaaattt
      301 ttgtgtcaaa accatcattc ctaagatgtt gacttcaaac atatcgtatg cttttccaag
      361 gtcgaatgta aggaagtctt cgaaaatccc aaaacccgca ttgtttacca gaatatcgac
      421 gcgcccgact tcttccatca ccttttcata taattcgtcc acgctttcag gattgctgac
      481 gtctaccttg aaagcataag cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc
      541 ttctacaaat gccattcttc gtgcgcaact tataacgatt gctccttgct ttgctgcttc
      601 ataacagatt tgtgcgccca gaccgcctga actacctgtt actaaaacaa ctttatcttc
      661 taaattcatg cctcttcctc ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata
      721 atcgtcgtgt tttcaaacac ttcttgtgct tctttttcaa gctctaaagc tgcttttcct
      781 aaatatctcg cactgatatg agtaagtagc agccgttttg cctgcgcttc ttttgcaact
      841 tcagcagctt gatgagtcgt ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta
      901 ttaaatgtgc tttcatgtac cagaatatcg ctattttccg caagcacaac ggaattatgt
      961 gtttttctcg tatcgcctaa aatagtcacg attcgtcctt ttttatctgg acctacgaaa
     1021 tcttttccat tgatcgtccg cccatcttct agttggatcg tttcaccttg cttcagtttg
     1081 ccgtacagtg gtccagctgg aatacctaat tcttttagac gatccacctg caattctcct
     1141 ttatgatcat gttcaaccac acgataacca aaactgtcga tcccatgatt tagttttttt
     1201 gtataaacag aaaattgctg atcagtaaaa atcggggctg tttcatttag ctcaatgaat
     1261 ttcagcggat aacttaaacg cgtttgcgaa atacctaaag agacttttat gtattcctct
     1321 atcccttttg gcccgtaaat ctctaatggc gtatccccac cttgaaatga ccggctgctg
     1381 atgagcccgg gaagtccaaa aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg
     1441 atttttctcg gacgaatagt cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg
     1501 aacaaccaaa cttcattgcg ctcgtctaaa agtttcaatg ctatacttgt cacatttcta
     1561 tgttttgcag gaacacctgc acctgttcct aaaaattgta attccataat ctacctgact
     1621 ttctttaagt tcctctttaa ttttagggta aaaagaaggg ataatcaacc gtaagtcgag
     1681 aatgggttca aattaaaaat aacgttctaa agcgatagcg ttttctttga aaattactat
     1741 aaaaacagca gcgaacccgt ttgagatcct ctgctgtttt ttattaattt gaagatccag
     1801 aactttcatt gtcgttggaa gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt
     1861 ctttcgcatg attttcagct tctttcgcat catgcacatc tttttcgaca tcttcataat
     1921 ccaagcgagt aatttctccg cctaattcca tcattaccaa ttgattcagt gggcgattgt
     1981 cctcttcatc agcaatcaag atcagtcctg tagctccttc accgatttta tttcctacaa
     2041 agtcgaacac ggtctgggca tcttttattt ctttggcatc ttgtgtcgca ccgatcattc
     2101 ctccggcaaa ccatcctaat aaaatcccta gcggaccacc taatacacca accaacattc
     2161 cgattaatcc acccgtagaa gatttgttat taccggtaaa gtcgataaaa tcttctattt
     2221 tgaattgatg tgtcccatta ggatcatggc tgacaactgc catctgttct cctttaatcg
     2281 aacgaacagc atgtaacttt ttgatttctg aaaatgcctg atatgcctgg ctttctactt
     2341 gaaaatttaa aatgattact cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt
     2401 atttatttta cctttaattg aaaaataaga aaagaaaagc actctttttt tccaataaaa
     2461 tagggctgtg acaaaagtcg tatcacagct cctttttccg aataaacggc gttcttgaag
     2521 ctgatcactt ctgtcacaac ctcccttaaa tctgaatcta tattttttgg tatttgtttt
     2581 tatttgcttg cctattcgac aaattcaaat tcaaactcac cgatacgtac aaggtcgcca
     2641 tctttcgcac cgcgtgcccg aagagcttca tccacaccca taccacgtaa ttgacgagca
     2701 aaacgcatca ctgtttcatc atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc
     2761 tcaccagata agatccacgt tgcatctgaa tcacgatcga tttggaactc aggaccttct
     2821 ggattgaatc catagtgtac agtttcttct tccagttctt cttcgtacaa tgggaattct
     2881 ggcgttactt ctaacaaatc tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc
     2941 actccagaaa ttggaaatac tgggatgtca tcagcaaatt catcttcttt ttccttgttc
     3001 agctgttctt tgaatttcac tagattttcc ggtgcatccg gcatatccat tttgtttgcc
     3061 acgatgattt gcggacgctc tagcaagcgt aagttataag tagataattc tttgttgatg
     3121 gcaagatagt cttcataagg atctcgtcct tccatcccac tcatatcgat cacatgtaaa
     3181 attactcgtg ttctttcgat atgacgaagg aattgtgtac ccagcccaac accttgcgaa
     3241 gctccttcaa tcaagcctgg caagtctgct gcggcaaaac ttctgccatc acttgtagta
     3301 accattccta gattaggtac gagcgtcgtg aaatgataag cgccgatttt tggacgagca
     3361 gatgaaataa cggatagtaa agtcgatttc ccgacagatg gaaaacctac aagtccaacg
     3421 tctgccaata ctttcaattc tagttcgatt tttcgctctt gtcctggttc accgttttca
     3481 gcaatttccg gtgcaggatt ccgaggagag gcaaaacgga tattccctcg tccaccgcgt
     3541 ccaccttttg ccacaactag tgtttgacca ttttcaatta aatctcctaa aagagcacca
     3601 gtctccgcat ctcggacagt ggttccttgt ggtactttaa cataggtatc ttctgaacca
     3661 cgaccgtgca tccctttact catcccattt tcacctggct gcgctttgaa atggcgattg
     3721 aaccgaaaat ccatcaatgt acgtaaccct tcatcgacga tcaagatgac atcgccgcca
     3781 cgaccaccgt ctccgccagc cggtccgccg tcaggaacgt atttttcgcg acgaaatgca
     3841 accattccgt ctccgccctt gccagctttc acatcgatcg ttacttgatc taaaaacatg
     3901 gacataatta agtcctccta ttattcattt atttatttga ctgcctgaat ttccacaata
     3961 aacaaaagtc ttacttattt tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa
     4021 gattgtttac cttcttcttt ttttatcatt tggtaaggtt tccaaccttt tttctgctac
     4081 caagtataat tttttgacaa aatattcttt gcgtagcaga ataacagtgt attaattgta
     4141 ttaattgagg atgccgtttc acagcacccc taataaagga ggaacttttg atatgacagt
     4201 aaaagtaggt attaacggat ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga
     4261 agtctcagat gatattgaag tagtagcaat caacgactta acaagtccaa ctatgttagc
     4321 acaattattg caatttgact ctactcatgg tacttatcct ggcacagtta ctgcaacaga
     4381 agactcaatc gtagtggatg gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa
     4441 aatcccttgg gcaaaagaaa acggtgtaga tatcgtacta gaatgtactg gtttttatac
     4501 atctgaagaa aaagcaaaag cgcatttaga tgctggtgtc aaacgagtag ttatttctgc
     4561 cccagctggt gctatgaaaa ccatcgtcta taacgtcaat gatgatacat tagatgaaaa
     4621 cgacaaaatc atctcagcag gttcatgtac aacaaactgt ttagcaccta tggcttattt
     4681 cttaaataat gaatttggga tcgaagttgg tacaatgaca acagttcatg cctacacttc
     4741 cacacaaatg ctcttagatg gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc
     4801 tgctgacaat actatcccac attctactgg tgctgcgaaa gcaatcggct tagttatccc
     4861 tgagctacaa ggaaaattac aaggacacgc acaacgtgtt ccagtcgttg atggttcatt
     4921 aactgaattg gtttcagtat tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat
     4981 caaaaaacac acagttgaca atccttcatt cgggtatgat gaccgtcaaa tcgtttctag
     5041 cgatgtcatt ggaacaactc aaggttcgat ctttgaccca acgcaaacag aagtaacaac
     5101 tgctggtgat ttccaacttg ttaaaactgt tgcatggtat gacaatgagt atggatttac
     5161 ttgccaaatg attcgcttac tagaaaaatt cgctaacttg taatttgata gatcaatcga
     5221 taaaagaagc ctttgactgg atccagtcaa aggcttcttt cttttcatcc cctactttta
     5281 aactcgttca atgaacatat tggcttaaaa attgttggac tacttgctga tatttttcag
     5341 gatctgtctt gtatgcttcc gcatgctcgg ctccttttac aacatacttt tcttttggtc
     5401 ccttcgttgc acgatagacc ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc
     5461 catgtataaa gagcattggg cgctggtttt tcttcaattg ttccactgca tttgcttcac
     5521 cgaaaaagta gccagcacgg acttttgtca ctagactcgt tacttgcata agcggaaatg
     5581 ctggaagact aaacatatcg tctagctgat aagcaagctc atcatgaacg gaagaataac
     5641 cgcaatcttc aatgattgct ttcacattat ctggtagttt ttctccgcta gtcatcataa
     5701 ctgttgctgc tcccatgctg acaccgtata gaacgatttc ttgcgatttt ccattgtttt
     5761 ctagcacttt gtttatccac tgtacatagt ctttacgctc tggccatcca aatccaatgt
     5821 aatccccttc gctttttcca tgtcctcttg catcaggaac aaggacattg taacccatat
     5881 catgaaacat ctttgcgtaa ttggtcatcg tttcagcgtt tcccatatag ccatgagcca
     5941 caagagctgt tttttcactt ttcgtttcag caggaagata aatcgctgat aatttcagac
     6001 cgtcttttga agtgatggtc caatcgcttc ggttttcttt tgaactaaac catttatgtg
     6061 catccgtttc tttggatgtc tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct
     6121 cagaaggcac aacagcataa ttataaaaat aattccctgc aaacactaac gcaacaatca
     6181 gagctaaagc gacgcctaaa aggattttca tccaaagttt cataaatcat ccatctcatt
     6241 tctctttttt catataacct tgaattagta taatcaaaga atctctacta tgcaatgttc
     6301 atttttataa cccgcgaatt gtaaaattaa gttagaaaaa taaaaaggca tttatggtac
     6361 actcaaattg tatttccgac gaaagaaaac aaaggagtgt aaccataaat gacctacaaa
     6421 catcttacca tagacgaact gacaatgata gaatcatatt atcttcaaca taataagccg
     6481 gttgaaatcg ctaaccgaat gggacgtgct atacaaacta tttataatgt agtcaataag
     6541 ttcaagcaag gcaagactgc tcttgattat tggcaccagt ataaagaaaa taagaaaaaa
     6601 tgtggtagaa aagtcattca attacctgct catgaagtag attacattaa agagaaagtc
     6661 actcttggtt ggacgcctga cgtcattatc gggcgaaaag aaaggcctgt ttcatgcggt
     6721 atgagaacac tttatcgttt attttctaaa ggaatatttg atattgacac actaccgatg
     6781 aaaggtaaaa gaaaacccaa tggccatcag gaaaaacggg gaaaacaaca gtatcagcgc
     6841 tcaatccatg atcgacctga taattatcct catttcaatt ctgagtttgg tcaccttgaa
     6901 ggtgatacga tcgttggcat tcatcataaa agtgccgtca ttactttagt tgaaagatta
     6961 tctaaagtca ttatcacgat taaacccaac ggccgtaagg cattagatat tgaaactgcc
     7021 cttaatcaat ggttttctcg cttccctaaa aacttcttta aatctattac gctattacgt
     7081 ttgactgtgg aaaagaattt tctaactgga aagccattag taaccaacat gatattgata
     7141 tatattttgc ggaccctgga acaccttctc aacgcccatt aaacgagaat tctaacggga
     7201 ttctgcgtcg taatggactg ccgaaatcaa tggattttag agaagtgaat cagacattta
     7261 tttccagtgt cagcaatcaa cgtaatcata ttccaagaaa atcattgaat tacagaacac
     7321 caatcgaaat atttttgagc tatgtacaag aagcatttta ttctagctta atttgacaaa
     7381 tcataaataa taaagaattg aacttctgag aaaacaaaga atcttttttc tttttttgaa
     7441 ttgactcatg gattggcgtt aaaattttat tttgaaaaag ttatttctgg tttaactcaa
     7501 aaaaagagat atgaatctaa tagtgataga aaggatgtta tttttcatga aatggttgtt
     7561 ttagatttaa tacatataac taataaatgc tattgaatgt atttaaacta attaacaaaa
     7621 gaatttgaat tgttaataaa atccaataaa atatattaat aaagtagata gaaataaaaa
     7681 aacaaagact aaacatatat aattggatta aacgcaatat ctgcaaaata ttgtaaattt
     7741 aaagcctttg tagaaaaatt tactgaaaga caaactgata agattattca gtatcatatt
     7801 acctatactg attaaaattt tttgaaacgg gatactaaat aaaaaattta tttataacag
     7861 aactaattat ttaatgcgga tgcgccaata cttggtcctg caagagctat ttattttg
//
LOCUS       DACJGB010000101         7750 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.105, whole genome shotgun sequence.
ACCESSION   DACJGB010000101 DACJGB010000000
VERSION     DACJGB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.105"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IYI34_002682"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IYI34_002682"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAR0560509.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IYI34_002683"
     CDS             complement(2032..3273)
                     /locus_tag="IYI34_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAR0560510.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IYI34_002684"
     CDS             complement(3291..4214)
                     /locus_tag="IYI34_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAR0560511.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IYI34_002685"
     CDS             complement(4435..6927)
                     /locus_tag="IYI34_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAR0560512.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IYI34_002686"
     CDS             complement(6934..7401)
                     /locus_tag="IYI34_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAR0560513.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IYI34_002687"
     tRNA            complement(7537..7610)
                     /locus_tag="IYI34_002687"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IYI34_002688"
     tRNA            complement(7668..7741)
                     /locus_tag="IYI34_002688"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2154 a         1690 c         1246 g         2660 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa
//
LOCUS       DACJGB010000102         7641 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.106, whole genome shotgun sequence.
ACCESSION   DACJGB010000102 DACJGB010000000
VERSION     DACJGB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.106"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(124..648)
                     /locus_tag="IYI34_002689"
     CDS             complement(124..648)
                     /locus_tag="IYI34_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560514.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(641..1114)
                     /gene="tsaE"
                     /locus_tag="IYI34_002690"
     CDS             complement(641..1114)
                     /gene="tsaE"
                     /locus_tag="IYI34_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAR0560515.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1237..2217)
                     /gene="pta"
                     /locus_tag="IYI34_002691"
     CDS             complement(1237..2217)
                     /gene="pta"
                     /locus_tag="IYI34_002691"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAR0560516.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2370..3050)
                     /locus_tag="IYI34_002692"
     CDS             complement(2370..3050)
                     /locus_tag="IYI34_002692"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAR0560517.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3176..4045
                     /locus_tag="IYI34_002693"
     CDS             3176..4045
                     /locus_tag="IYI34_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0560518.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4071..4586
                     /locus_tag="IYI34_002694"
     CDS             4071..4586
                     /locus_tag="IYI34_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560519.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4633..5196)
                     /locus_tag="IYI34_002695"
     CDS             complement(4633..5196)
                     /locus_tag="IYI34_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560520.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5251..5430)
                     /locus_tag="IYI34_002696"
     CDS             complement(5251..5430)
                     /locus_tag="IYI34_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560521.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5507..6016)
                     /locus_tag="IYI34_002697"
     CDS             complement(5507..6016)
                     /locus_tag="IYI34_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560522.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6112..6492)
                     /locus_tag="IYI34_002698"
     CDS             complement(6112..6492)
                     /locus_tag="IYI34_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAR0560523.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6822..7571
                     /locus_tag="IYI34_002699"
     CDS             6822..7571
                     /locus_tag="IYI34_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAR0560524.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2173 a         1585 c         1358 g         2525 t
ORIGIN      
        1 cagctgattg aacaccgttt attcaaaaaa aatggactgc gacacgactt ttgtcacagt
       61 cccttcgtgt gtttttctgc cttctcaaaa ggatgacagt ttctcactct tttcctattt
      121 cttctattca atcaaataac tcattttata tacatctaaa aattcaccta gatctgttct
      181 tgcccctcta ggcatgaccg cttcgatttg gaagcccatt tttcggtaaa ggtgtacggc
      241 tctttcatta cgaacttgaa cgtcaagctc caatcgaaaa agtacaccca tttccttcgc
      301 ccaactgatg atttcttcta gcataagagt gcctagtccc attccccaat attcctgcag
      361 aatactgata ccgacttctc ccacgtgaga caaacgaaat tgactgtctg ccttgacaga
      421 tgctgttcca ataatcgtcc cttcatagat tgcaagcagc aataaattat tattgctttc
      481 gtatatataa tccaactctt cttctaaggt ttctggtggt aacaggagtt ctgcttcatc
      541 caaaactaaa aattctgtct ccttattaac ttgtcccatc atcaaaagaa tgtcttttgc
      601 atctcctggc actgcttccc gcaagatgat cccaacttct tcactcatga ttttcagccc
      661 atttttctag aattcgctgt ttgaaggttt cactttgctc cccgtatgag attagtttca
      721 cataccgctt ttctaaatct gtatcacttt tttctaaaat cagttccaaa taatcctccg
      781 gcagtgcttc ttctaataga tttccccatt caacaacgga aagcccatca ccttcaaaat
      841 attcatctaa ccctaaatct gctccgctag ctgctacccg ataaatatcc atatgataca
      901 acggaatccg accttgacta tattctctaa taatcgtgta tgtcggactt ttgatcattt
      961 gcgttatccc taatccttgg gcaattcctt tcgtcaacgt tgttttaccc gcacctagat
     1021 caccagtcaa tatcagattg tcacctggca ttgcggaatg accaattgcc tctgcgaatt
     1081 gttctgtcat tgttaatcct gatagttcga tcatttttca tcttctcccg ctcttctttc
     1141 tttttgccag tatatcgcgt aatgaagttt ttttaaagaa aaaatgaact agtcaagaac
     1201 agcagctgct gtttttggct agttcgcgtg tatttgttaa tcaatcaatg tttgtgctgc
     1261 tgtgataatc gataatttat atacatcttc ttcatttgct ccacgagaca aatcagaaac
     1321 cggtttgttc aagccttgta gaattggtcc gattgcttca aagttaccga aacgctgagc
     1381 aattttatag ccgatatttc ctgactgcaa ttctggaaat acaaagactg ttgcttgacc
     1441 agctacttct gaatctggcg ctttcagttg tgcaactgaa ggaacataag aagcatcaaa
     1501 ttgcaattcg ccgtcgatca tgtattgtgg cgctagtttt ttagcgattt tagttgcttc
     1561 aactactttg tctacttctg gcgatttcgc tgagcctttt gtcgagaagc tcatcattgc
     1621 tacttttggt tcgatatcga acaattctgc tgtttttgca gaatctacgg cgatttcagc
     1681 caattcttgc gcagttggat tcacgttgat cgcacaatcc gaaaagacat atttttcttg
     1741 atctcttcca cgtaccatga tcatcgctcc gcttgtccga ctgatgccag gctttgtttt
     1801 gatgatctga agtgctgggc gaacagtgtc gccagtcgaa tggatcgctc cagagaccat
     1861 tccgtctgca atccccatat acgtaagcat cgtaccaaag taattcacat cttttaaaat
     1921 cttttctgct tgttctttgg atgcctttcc atttctacgt tcaacaaaag tatccaccat
     1981 ttcatcccac ttatcgtaat tatcagggct gatgatcgta aagctagatg ttttgattcc
     2041 tctggcatgc gctgcatttc ttatttcttc ttcgttccct actaaaatgg gttccacaag
     2101 ttcttctgct ttcaaccggg cagctgcgcc taaaatacga gcatcagtag cttccgggaa
     2161 aacgattttg atcccacggc gaacaatttt aaatcttaaa ctatcaaaca attccacaag
     2221 atgacctcca gacgttttta tatgtattat catacactat tccagaaaaa aagaacagtt
     2281 tattttatct gtatcagtca aaaatgctgg aaaaattaag tttttcacat acaaagcgaa
     2341 ctgcactata acaggaaagc gctgttttat tacactgttt caggcagttg ccaatcaatc
     2401 ggttcttctc ctaacgcaag gagcgcatca ttcgttttcg agaagggttt ggaaccaaaa
     2461 aagccacgac ttgcagataa cggactggga tgtggagatg ttatgatgat atggcgactc
     2521 ttatcgatca ttttgatttt ttcttgtgcc ggttttcccc acaagataaa cacgattggt
     2581 ttttcccgtt catttaattt ttcaatgatc gtgtctgtta atctttccca acctttcccg
     2641 cgatgagaat acgcttgccc ttcccgaacg gtaagaacgg tgttgagtaa aaggactccc
     2701 tgtttagccc atgacactag atttccatgt tgaacaggtg cgattccaag atcatcataa
     2761 agctctttat agatatttct taacgatggc gggatcttta cgccaggttg cacagaaaaa
     2821 gataagccat gagcttgatt ttctccatga taaggatctt gtcctaaaat caccacttta
     2881 acttcttcat aaggagtcaa ttccaatgct tcatagatat gatacatatc tggatggatc
     2941 ttttgggacg catattcttt ttttaaaaat tctcttagat gcagataata atcttttgaa
     3001 aattctgagg acaaaacttc ttgccaactg ttatgaatga tttctttcaa aaactccacc
     3061 tcctaataaa aagaataact cacattttct tcaatcacaa gggacaatct tcaccctcag
     3121 cacttgatta atatggtaaa ctaggaacaa gaagcagaaa ggaacgataa tcattatgat
     3181 caaattaatt gcatcagata tggacggcac attattagat gcccatatga gtgtttctcc
     3241 tgaaaatgcc gaggcgatac gttttgcaaa tgatgccggt gttgagttca tggtcgctac
     3301 tggtcgaaac tatcaagaag ctcgcgcagc tttagatgag gtaggaatcg attgtgcgat
     3361 gatcactttg aacggggcac aggtatttga taaagaagga aattcattgt ttaccgtgtc
     3421 gattccaaat aatcaagcaa tctctgtttt agatattcta gatgctaacg gtatttacta
     3481 tgaagtagca accaacgatg gtctttattc tgaaaatcaa gcgaaacgaa ttgaaagttt
     3541 tgcttccatg gtggctaccc atttacctca ccttacgtat aaaatggcga ttgccatggc
     3601 ctcagctaag ctggagttat tgcacatcac atacgtggac agtattcgtg ctattttgga
     3661 agaaaagaat ctggaagttt taaaaattat ttgttttcat acagaagggc cagctgtatt
     3721 aggtccagtt ggaaagcaga tttctctttt aggcgatcta gccgttactt cctctggtca
     3781 aaacaatatc gaagtgaacc ataaaaacgc tcaaaaagga attgctgttg cccatgtcgc
     3841 tcacgagcgt gggatttctt tagaagaagt catgacgatt ggcgataact tcaatgatgt
     3901 cagcatgctg caagtagcag gagtcagttt cgcaatggga aatgcagaaa tcgaagtaaa
     3961 agattatgcc aaatatacaa cggacacaaa ccttgaatct ggcgtaggaa aagccatatt
     4021 gcgcgcgatt gcagaagact tgtagtttta tctagaaaag agggatatag atgtcagaat
     4081 ttttcattca ggaaaagcag cttggacgtg caactcggac aattgtcaaa aacgaaaaag
     4141 gacattccct gtttttgatg gttggtcgct ggggaacaaa gggcgatgca ttgtctcttt
     4201 atgccatgaa tggcgatctc gttgctcaca tcaagcagat aagcctgaca ttcggtacgc
     4261 gttttgagct gtataaagag ttcgaaaagg tgggaaccat gcagaaaata ttcaattggc
     4321 ccggtgactt ttattatatt cgtagacttc actggacagt ccaaggtgat atctataatc
     4381 atcaatatca aatccatcat ttcaatcagc aaatcatgaa aatggataaa gcaacacttt
     4441 tttcaggaga ttactatgta ttggatgtct caaatgatga agatgctcca gtttgtattt
     4501 gtattgctgc ggttttagat tactggctct accgtaaaga caaaagtcgc gataagcctg
     4561 tttcttaccg acttaactta agttaaaaag atgtacctga aatgcggaaa tacgcatttc
     4621 aggtacatct ttttagatat tcttttgcat atgatcataa agatgaccac tgatcatccg
     4681 ctgaccgaca actttgaagt catggcgttc gtataatttt ttagcctttg gatttttttc
     4741 atccacactt agaccgatga cggagcgatt agctttcttc gccaatttag gtaatgcttc
     4801 taataatcgc gaaccgatgc cttgtcctct aaaatcttct gatacactga tagagtccaa
     4861 ataccattcg ttgggaaacg tttctttatc aatgaacaaa cgtgtagacg catctagctg
     4921 atatttctct aagataggta ccagtggctg atcgatgatt gattcttctt catctgtata
     4981 accgtaagca actcctgcaa ctttaccgtc tatttcttct acgatacctc gtgaataact
     5041 gtaacgataa tttggatcag tgattgcttc ttctaaaatc tctaaagtct tttgttcccc
     5101 tgtctgcttc aaaaatggca gttccatatc ttttagaatc actaagatca atggggcgat
     5161 ttctggagca tcttcttttc tcgcaaaacg aatcatagtt tcctctcctt ttctccattc
     5221 taacatgcag aaaaagaaat cgcaaaatcg ttattttaaa aaatcttcaa tcttttcagt
     5281 aatgaaataa ccatgagtaa cgattcttgc cgcttcagtt cggcaataag ggcagacgac
     5341 tggcatttct ttatcattat gttcgactac attattgact tcttcgtatg caaatttttt
     5401 tccgcaatta gggcatttca ttttttccat gttatctcaa cttcctttca tctttactct
     5461 cttagcttac tcttatattg taaaaaagag aagcaatatc cttcttctat gcagatatcc
     5521 atacctgacg gtcctgttct ccatctatta atcggacttt ataataagga cgttcatctt
     5581 tgactagaag tgtccactct ttcacttttg cttttttccc gtagtagctg actgcttcct
     5641 tcatcgctgc ttgcggttgt ttgaactctt ccgttttttc tatatcaaaa agatgttctg
     5701 cggccttttc cgtgtcattt cgtacggttg ttttcccagt ggatggattg actgctatga
     5761 cttgtccttc acaaaaaaac tggtattcat atgttgcatt cgtaggattc tccaaatctt
     5821 tttcaactgc aaaatgaata gcagatatgt tatgttcttt tgtttcgttt tttacgattg
     5881 ctgctgcttt tttcaatgag atccgatatt cctggtcact aatatcatcc atgtgaaaag
     5941 cggcattgag cagtcccgtc ataccggtaa tccctattac gatgagggca gcaacaccaa
     6001 acatcatttt tttcattctt cattctcctg tttctttttt ttaaagaata tcaagaaatg
     6061 aacgaatgaa aaatcgaatg aaacgaaaaa aaagtgacaa ttttgttatc tttattcgtg
     6121 cagttctaat ggcaatccgt ctggatcaaa gaaaaacgtc attttttttc ctgtaaaggt
     6181 atcttctcta atcggttctg attcgatccc taaggactgc aattctgcaa ttgtttcttc
     6241 aatatcgtct actttgaaag caagatgacg caagccacaa gcttctggat agtttggtcg
     6301 ttgtggcgca ttagcaattc caaaaatctc caattcagca cttcctagtt tcagatctaa
     6361 tttatagtca tctcgttctt tgcggtaatt ttctctgatt acttcaaatc ctaatttatt
     6421 aacataaaaa tcacgtgatt tctgataatc tgaaacaatg atggctatat gatggatcgt
     6481 tgtaaggttc atatgagcac tcctattctt cgtttaagct cggatctata ttaatctcca
     6541 gttccattgt aaatgtgtca gattgaaaaa aatagctgtt tttccttttt ataagaaaaa
     6601 aggcaaaaaa aacagaaagt aggtcctcca agacactctt cctgttccca atataataaa
     6661 tgaaagtaag aaattatatt ttcaagaaaa caaacagaaa attatcataa cacaaattat
     6721 ttataattga aagactagtt ttttttcaag aaaagcatac aatagattgt gttagtaaat
     6781 acagaacaca ctacatttag tgtcttaagg agtcctcata catgaagtca tattttcagt
     6841 ttttagctca tcagctaaaa ggctggccgc aacaaaatta ttatcttttc tttttcagtc
     6901 ttggctgcca ggtcatgact cttgtcaata gtcccatcac ttggctgtcc gttattacat
     6961 ttatcggtac cacgcttggt gtattgtgta ttttatccat caatgcggca aaatcagtca
     7021 acggtatttt aggtattttg tctgcgattt gttttattat cgttgggttt tcagcaaaga
     7081 actatttaag tatcggtgaa cagattgctt atatgatcac attggatatc cccgtattgt
     7141 taagtaaaga atggaatcat aatatggctt caaaaatccg aaaattcact ggaaaaagct
     7201 ggtttatcgc aatcatcagt actttccttg tctatcttgt ttcaggattc ctgattggtc
     7261 gttatacaga tgatcctcgg ccgtgggtag atgccattgc tttttctatt agcttgactg
     7321 ctggtatcat cagtttcttg cgttacaaca accaatatta ctggtggtta gcttcagggc
     7381 ttgctcaaat ggtcctttgg tatatttcat ttacgcatgg atctgcaagt ttggcgatgt
     7441 tcgtgaacag ctctgtttac ttgctgaatg atgtccttgc atttaccgtt tcaccttggt
     7501 ataaccaaaa agaacgtgaa cgtatgcaaa aaatggaaga agaatactac aacgcacaac
     7561 aacacgcata aaaaaactga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct
     7621 aaacacgtat aaacggcgga a
//
LOCUS       DACJGB010000103         7474 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.107, whole genome shotgun sequence.
ACCESSION   DACJGB010000103 DACJGB010000000
VERSION     DACJGB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.107"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            275..1753
                     /locus_tag="IYI34_002700"
     CDS             275..1753
                     /locus_tag="IYI34_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAR0560525.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            2030..4090
                     /locus_tag="IYI34_002701"
     CDS             2030..4090
                     /locus_tag="IYI34_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560526.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            4173..4742
                     /locus_tag="IYI34_002702"
     CDS             4173..4742
                     /locus_tag="IYI34_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560527.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(4917..5723)
                     /locus_tag="IYI34_002703"
     CDS             complement(4917..5723)
                     /locus_tag="IYI34_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560528.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            5861..7375
                     /locus_tag="IYI34_002704"
     CDS             5861..7375
                     /locus_tag="IYI34_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAR0560529.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         2683 a         1226 c         1459 g         2106 t
ORIGIN      
        1 taaaagagca ggacggtggc cgtcctgctc ttttttgtgg ttgtttacgc aaatggcgca
       61 aatcttgtac attttaacac aataaaacaa aaaatataga atatttcctc ataaattttt
      121 ccattttttg caatattctc aaaaaaaaga taaaaaatga gaataactga tgaaaaaaca
      181 agcataatcc atcataaaat cagtataggt gaaaagatga actttcatct gcatatcaag
      241 taattggtat gaggcatttt ttgaggtgta ggatatgaag ttggattacc ttatagaaga
      301 ggaagataga gaaaaactgc tgctagctag atatttagaa tttgagaaaa agaattatgt
      361 agtgctgcct aagttatatg aaacattagg tttgtctaaa tttaaaacaa gaaattatat
      421 catagaatta aataaagaat ttactgaatt caaaacaaag ccagagatcc aaatttcaga
      481 agaagcagag atccatgtcg aacgtctgaa tttgacggat atcaaaaatt taagaagaat
      541 ctactttcaa cgatcaaaaa ttgctcgact gttaacggag atcgtggatt caaaagtaac
      601 gatcgaaaaa tttgcagaga atcatttttt aagcatatca agagcctatg taaaaagaag
      661 agaattagtc tcttttcttg aacagcatca aataaagtta aagaaaaaca tattagttgg
      721 ggaagaaata aacatacgga acgtattatt tggggtatat ttcggggtct ttaatggttt
      781 ttctatgcca tttagagaaa gcgtcatgaa tgaagcaaag agtatcgtgc aatattttga
      841 atatctgttc aatttaaaac tgtctgcaac aaaacgaatc aaattcaatt tgctaaccac
      901 tattagtttg ttgagggcct atgggaatca accaatcgga aaacgtttct tttcagatga
      961 atactttaag agctcagaag gaatgaagat catcaaggat ttatcacata tttcttttct
     1021 tcatatccct aaagagcaga tcattgatga gatttcttat atcatgttgt ttttgatcac
     1081 ggaagaagaa tttgaattag atggcgcatt tggacttgcg gaagctcatt ttcatgatat
     1141 ccacatgatt agtcaaaaaa tgtcagtgga attgattgat caattaccat tttcgattga
     1201 aatctctgaa gaagaaagac tatatatacg aaaagaactt cgtatgggta tgatgcatgt
     1261 caatcgaaaa aattggatat tcgactttga gatgactagc tttacgtcta aaaagcagct
     1321 acagtttttc ttcgagacgt atccaatatt cagtacagca atccaaaagg tcatacaaga
     1381 gaataaacgc tatttcttgg gaaaagatga acgtttcatg attcgcttat tttatgatta
     1441 tctcttttta ttggtcggta tttgtccggt atcatatttt gaagcaccga ttcatgtctg
     1501 tattgatttt tcgcaaggat ctgcttatac cgattacatt atttctcaag tgaaaggatt
     1561 taaaaattac aatattgtga ttgagagtcg aatcacttct cgtacgaata tttatttatc
     1621 tgattgttta cttgaaaaat ttaataaaga acagataatt tggaaaaatc caccgactcc
     1681 agatgactgg gaatattttg gaaataccat catcagagaa aaaaatagac atttaactga
     1741 aaaaatcagc tagtatttat ggatggcagc tttgtctcac atatttatta gctgatttat
     1801 gtttttttca aagaaaaggt aactagatat aactagatat aactagatat attacttaac
     1861 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
     1921 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
     1981 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
     2041 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
     2101 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
     2161 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
     2221 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
     2281 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
     2341 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
     2401 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
     2461 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
     2521 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
     2581 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
     2641 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
     2701 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
     2761 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
     2821 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     2881 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     2941 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     3001 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     3061 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     3121 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     3181 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     3241 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     3301 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     3361 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     3421 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     3481 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     3541 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     3601 gatagatcaa aaagaacaaa aaagacaaga agaaagcttg tcagaaaaaa aacaacaaat
     3661 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     3721 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     3781 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     3841 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     3901 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     3961 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     4021 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     4081 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     4141 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     4201 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     4261 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     4321 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     4381 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     4441 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     4501 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     4561 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     4621 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     4681 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     4741 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     4801 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     4861 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     4921 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     4981 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     5041 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     5101 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     5161 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     5221 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     5281 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     5341 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     5401 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     5461 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     5521 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     5581 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     5641 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     5701 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     5761 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     5821 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     5881 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     5941 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     6001 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     6061 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     6121 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     6181 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     6241 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     6301 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     6361 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     6421 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     6481 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     6541 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     6601 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     6661 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     6721 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     6781 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     6841 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     6901 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     6961 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     7021 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     7081 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     7141 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     7201 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     7261 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     7321 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     7381 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     7441 ttgaacactg tttagtgaag aggttgtgaa aaag
//
LOCUS       DACJGB010000104         7442 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.108, whole genome shotgun sequence.
ACCESSION   DACJGB010000104 DACJGB010000000
VERSION     DACJGB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.108"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IYI34_002705"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IYI34_002705"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAR0560530.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IYI34_002706"
     CDS             complement(2260..2511)
                     /locus_tag="IYI34_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAR0560531.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IYI34_002707"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IYI34_002707"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAR0560532.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IYI34_002708"
     CDS             3461..3679
                     /locus_tag="IYI34_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560533.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IYI34_002709"
     CDS             complement(3968..4480)
                     /locus_tag="IYI34_002709"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAR0560534.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IYI34_002710"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IYI34_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAR0560535.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IYI34_002711"
     CDS             complement(6933..7367)
                     /locus_tag="IYI34_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAR0560536.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
BASE COUNT         2063 a         1636 c         1332 g         2411 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tt
//
LOCUS       DACJGB010000105         7357 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.109, whole genome shotgun sequence.
ACCESSION   DACJGB010000105 DACJGB010000000
VERSION     DACJGB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.109"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="IYI34_002712"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="IYI34_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAR0560537.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="IYI34_002713"
     CDS             complement(1804..2697)
                     /locus_tag="IYI34_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0560538.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IYI34_002714"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="IYI34_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAR0560539.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="IYI34_002715"
     CDS             complement(join(3472..4509,4511..4573))
                     /gene="prfB"
                     /locus_tag="IYI34_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAR0560540.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IYI34_002716"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="IYI34_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAR0560541.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1962 a         1634 c         1284 g         2477 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatt
//
LOCUS       DACJGB010000106         7204 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.110, whole genome shotgun sequence.
ACCESSION   DACJGB010000106 DACJGB010000000
VERSION     DACJGB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.110"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..206
                     /locus_tag="IYI34_002717"
     CDS             <1..206
                     /locus_tag="IYI34_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAR0560542.1"
                     /translation="KVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGY
                     RNQRLAQRILDNELGCANASEAV"
     gene            341..562
                     /locus_tag="IYI34_002718"
                     /pseudo
     CDS             341..562
                     /locus_tag="IYI34_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            562..909
                     /locus_tag="IYI34_002719"
     CDS             562..909
                     /locus_tag="IYI34_002719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560543.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            906..1955
                     /locus_tag="IYI34_002720"
     CDS             906..1955
                     /locus_tag="IYI34_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0560544.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1970..2176
                     /locus_tag="IYI34_002721"
     CDS             1970..2176
                     /locus_tag="IYI34_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560545.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            2197..2793
                     /locus_tag="IYI34_002722"
     CDS             2197..2793
                     /locus_tag="IYI34_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HAR0560546.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3745..4344
                     /locus_tag="IYI34_002723"
     CDS             3745..4344
                     /locus_tag="IYI34_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR0560547.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4393..5109
                     /locus_tag="IYI34_002724"
     CDS             4393..5109
                     /locus_tag="IYI34_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HAR0560548.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            5123..5902
                     /locus_tag="IYI34_002725"
     CDS             5123..5902
                     /locus_tag="IYI34_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAR0560549.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5985..6341
                     /locus_tag="IYI34_002726"
     CDS             5985..6341
                     /locus_tag="IYI34_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR0560550.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            6331..6597
                     /locus_tag="IYI34_002727"
     CDS             6331..6597
                     /locus_tag="IYI34_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560551.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6756..7034
                     /locus_tag="IYI34_002728"
     CDS             6756..7034
                     /locus_tag="IYI34_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560552.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(7031..>7204)
                     /locus_tag="IYI34_002729"
     CDS             complement(7031..>7204)
                     /locus_tag="IYI34_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAR0560553.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         2626 a         1028 c         1408 g         2142 t
ORIGIN      
        1 tgaaagtaga atttgtctat caatacacat ttgaaacctt acaacaattg gatttggagt
       61 tatttgacta tgtcaattgg tggaaccacc ttcggttgca cggtacactt ggctacgaga
      121 caccggttgg ttaccgtaac cagagattgg cgcagcgaat ccttgataat gagctcggat
      181 gtgctaacgc tagcgaggca gtctaacttt aactgttagc tcctgccgaa gatcgtcaca
      241 tccgaggagg ctcattgtca aggacaatcg gaatagcata cggaaagaag ttctgcacct
      301 tataaaattt gtcaaaaaaa ctgttgccat tccacttaaa acaactgaat ttgatattct
      361 acacgctcta atgtccacac ctaataaagt cttttctcgt agtgatttga tagagaaagt
      421 taagggaata gaatttgatg gactagatag gagcatcgat tctcatataa aaaacttaag
      481 acataaaatt gagaatgatg ctaaatcacc tatgtatatt ttgacagtcc atggtacagg
      541 atacagattt ggtggccact aatgagaaaa agaacgataa aaactcaatt agctgtttcc
      601 tttttagcaa tcgcaacatt aataatagga tcgattagtt tagtcgcatt aagtttgatg
      661 aacaatcatt ttagtaaata cgtggaagag cggcaagaag atttgttgaa tcagtatgta
      721 tatacaattg atttattgtg gttaaatagt ggggaaacat ggaatagtga agaacttgca
      781 gcactatctg agaaagtatt agagaataat atttattttt ctatagaaga cgaacaaggc
      841 aacatggttt gggaacttac tggaaaagat ttgaagtcag cacaggagaa actgaaaaaa
      901 atgcattgaa agtttctgag aaaaacagtg tgaaactaga cgaaacaata gaagtgaaaa
      961 agaagttaat taatgatgga aacgagtttg gaaaagtaac gttttattat ttcggacctt
     1021 ttgcttatac agagcacgat gccttgttta tctccagtat gaaacaaagt ttaatgtatg
     1081 ttgcaattgc agctcttctt gtatcattca tacttgcttc gtggatctct gctagattag
     1141 gattgccact aaagcatgta agcgatttta cacataaatt aactcgtggg gaatatgctg
     1201 ataaaatccc acaagaaact tcaattattg aaataaattc cttaattgat tccctaaatg
     1261 atttaagtaa tcagttggag aaacagcatg ggcttcgaaa aagattaact accgatattt
     1321 cacatgaatt aagaactcca ttagctacgc ttaaaggcaa tgtggaagga atgattgacg
     1381 gagtctggaa aataactccc gagcgcctac aatcttgtta tgacgaaata gatcgtctaa
     1441 ctcgtttaat tggaaatata gaaataatca acaaaattga agcaaaatat gatcatttaa
     1501 ataaaactga gtttaacata tataagttga ttgaatccgt tattgaaaat ttcgccagca
     1561 aaattgagtc aaaaaacctt catgttgaaa tccaagggga taacatcaat atatctgcag
     1621 ataaagataa aatgaaccaa gttgttatca atcttttgaa taatgctatt aaatttacgc
     1681 aaaaggaggg aaccattaag ttttctataa gtaaaaataa agaccatgtt cttttaattg
     1741 ttgaagacaa cggcatcggt atagaaaaag atcagcaact tcatattttt gatagatttt
     1801 atatggctga cccctccaga agtagagctt taggggggca aggtatcgga ttagctattg
     1861 taaagagcgt tattgaagcg cataaaggta gtataactgt aaaaagtaaa ttaggtctag
     1921 gaactaaatt tgttataaag ttaccatttc aataaaggag gagagttatt tgtttcaaaa
     1981 acagataaaa attgctaaac tagtatgtga atgttgggca aaaaaaatat tttttcaact
     2041 gatttcgata gatagtgtcg aaaaagttca tttagatatc ttatcaaatt ctgtagtaat
     2101 cttttctaag aaggacttat caatagctac aattaaaaaa gaagtttttg gattacaaat
     2161 ttatgagatg acctaaatgt gattaattaa aggaggatga agacagtgaa aaagatgaca
     2221 aaattagtat ttgcaagtct tggaattatt gtatttttag gagcgtgtag caatcaaagt
     2281 gaatcgaata attctaatag cacaaacgag gaatcgactt ctacagcgtc tagtgaaatg
     2341 aatagtatgg agggaatgaa tcacgaaggt atggttcctt cttcaatgaa agatgctgcg
     2401 aaccctaaat tcccggtggg aagtaatgtt atactgctag gcgatcatat ggaagggatg
     2461 aaaggtgcaa atgctcaagt tgtaggggcc tttgatacta caatgtatga agtaagctat
     2521 gagccaaaaa ctgggggacc aatggttaac aatcatcgat gggtagtaca agaagagtta
     2581 aaagatactg agactgtagc aaatgagggc gatactgtta tcttgaatgc cgatcacatg
     2641 gatgggatga tgggagctga agcaaaagta gataaatcta ttacaggaac tgtttacgtt
     2701 gttaattaca ctccaaccga tggtcaagaa gaagttaaga atcatatgtg ggtaactgaa
     2761 gatgagatgg aatatgacga aaataatgag taattttata tactaaacca acaagctggt
     2821 acatggtctt cgtattctca acatgaggaa tatgagctct ttgttaataa ggactgatga
     2881 gttgtgcaaa atcaaatctg ggtcagaacg aacctcattc tggctcagat ttttggctct
     2941 tcgacattta gtgctggtag agagacaact gaaaatttag tcataaccag ttaggcagag
     3001 tgcttaactg gtttttgttc gcatttatta ttcttttgat attggctgct tgcccttcaa
     3061 ttttatttgg tttcttaatc aactcttcat ataaccccaa atcatatatt aatgaggtgc
     3121 ctcctattcc tataaatcta tctcttcttt aatagtatga gaaaagtcta ctcttgtaga
     3181 tttaaattgt caacaaaaaa tttacagatg taaatgataa gttgaataga gaaagataaa
     3241 ttattttttt ggtattcata taaggttaat tatgtattat cacaaaatga ttgttgtcga
     3301 ataactaaaa gtgtcaaaac aatgatgaaa tcatttagtt cttataaatt agacaagaga
     3361 aaataaatat tccatatcta ctttattgga tccataacat gactaaattc tgaatacttt
     3421 ttaaagctta tcatttggta aaactgaata taagttaatt atatagactg aaaattttga
     3481 attttcagaa tagaataact taaaagtact gacaaatttt ttaaaggggc aatattaatg
     3541 ttctgagata atcaaatctt accgaaaata tcaaaagtcc aatgtgacac aattggattt
     3601 tgtcacattc gttaaaatcg ctccatcctt agaggctcta ataggaaatc acttttcttt
     3661 tagcgccttt tttctatagc ttgtttaaat gtgacttatt atctgttata atcgtattaa
     3721 gtaacatttg agaggtgtct aaaaatgaaa agcgtggaac cgattcgcga caaaaaaaag
     3781 atcgatgcca tgaaggccat tttggcttcc ggaaaatatg ggcaacgaaa tttggtgctt
     3841 ttttcgatcg ggatcaatac agcctatcga atctccgatt taagacagtt aaaattaagg
     3901 gatgtgctag aaatttcccg tgggcgggtg atcgtcaaag aacgtctggc catgaaggaa
     3961 caaaaaacgg ccaaacacaa ttcggtcttc atttcgaaca aattgcgaaa agtgattctt
     4021 gattatgttc aatccgaatt tcctgagcag ttgcaagcac aagacttcag caagtatttg
     4081 tttccaagtc gaaaaggagc agatacgcct ttgacccgac aaagcctttg gcggatcatt
     4141 catgaagcag gaactgcagt tgggttaaaa gaaattggtc cccattcgat gcgtaagacc
     4201 tttggctatt ttttatacaa gcaaggaacc aaaacagaga tcattcagtc gctgctaaac
     4261 cattcttcac aacgggagac cttacgctat attggaatca cccaggaaga caaagatact
     4321 gcagtaaaga gtttggattt atgacagaat aatgccctat ataatagaag gaaaaattca
     4381 agaggtgaga aaatgaacga agaaaagcag cctaactttc ctgataaata tcacttatct
     4441 cgcaaagagt ctgtgtattt attgaagaaa aacattgtag aactggtgta taatgcgggg
     4501 aaatttgaag ggttaaatac aacgttgtta caaacagaag ggattattaa gtataatcgg
     4561 gccaacaacg tggctgtaga tgatgtcctg accgtcgtta acttaaaaag aggctttgaa
     4621 atgcttttaa atgacgtaca ggagcccctg ttagagacaa gtaagcggat taatcggatc
     4681 gttgcagcag aggatgcgct ttttcccggt gaaatacgaa ccggtggggt agaattatcg
     4741 acgattcaag gaagatatgt gccaccaatg ttaactgaag acgaggtaaa caatcaatat
     4801 ggtgagataa tgaatcaaga gatatcagat acggaaaaag ccctgcgact gtttctattc
     4861 atttcgaaaa atcaaatatt ctggaatggt aatgtgggta cagccttgtt gattgcgaat
     4921 aagattatgt ttagcaacgg tttaggacta ttatcaatac ccgaaaatgt atttatgaaa
     4981 tttaacgaac ttttatcaaa gtattgcaac agtaatctat tagtcaatga gtcaaatatt
     5041 atatcatttt tatatgaaca gtgtatcttt ggtattaatt atttgaaaat aatccaacat
     5101 acaatttaag aggtataagt aaatgatgac tatagacgaa gcaaaacaat tagcaactta
     5161 tttttcagtt ggttcagcgg gatatatttt agactttact aatgtaacgt ggaacatatt
     5221 tacaaagaaa atcgtaggga ttgaaataca aaatcattat gggggctcaa agggagtatc
     5281 ctttcaaaag tttctgcttg acgatcaatt tgatgattcg ttaaagaatc ggttaatcca
     5341 atctttaaaa ggtattatgg atcgagataa aaaatatgaa tctactgaaa tgaatcccat
     5401 agttcagttg ctttttgagg aagtatttaa agaagatact tcaactgcat taagaaaaat
     5461 gaaattttca agcgagattg atttacttta tattcggcag ctacccatta ggattcaaac
     5521 agatattaaa aacaaggatt ttgactctgt tttgacaaaa gcaaatactt taatagatga
     5581 agttctaaaa tttattattg aaaataccga aggaccgagt gttaatatta atttatttaa
     5641 gtcaaaagaa ttaagaaaag aagtatttgg ccgcctcaat attaaaactg aaaaagatat
     5701 ggataataga atcaaagccc ttgttggagc actcaataca ttatcagata aaatactcga
     5761 aatgagaaat agtcaaggag atgctcatgc acatggctca aatagaatag taattaagga
     5821 agaagaagca attcttgttg caaattcttc tatggttttg tgcgagtatg tgtttactaa
     5881 gtataaagga ttaaatagat aataaaatgc atattttata gtatacaatt cgaattttta
     5941 tgttatatac tataatataa aatcaatata tgatataatt gggtatggaa aaaatatctt
     6001 ttgaaacatt taaaagaccc aatggtcatg atgaattttt agaatggttg gaaacattac
     6061 caaaaaaaga tagtgcgaag cttttacgta caatagaaga aacagaaaag aatgggatgc
     6121 tagtagctca acggttgaaa tgggtgaaaa aattagacac taatttgtac gaattaagaa
     6181 gtaaggtagg atcaaacatc caaagagcca tctatttcca tgtggacaat ggaaggtatg
     6241 tcattactca cggatttact aaaaaaacgg agaaaacacc aataactgaa ctcaagcatg
     6301 caaaagaact tagagaggag tggttcacaa atgatgatta aaaaattgat tgaagaaaag
     6361 aaaaaagata aagattttgc agaagcatat gaccgtgaag gaagaaaact cgaaactgcg
     6421 gttgctcttt atcatgctcg ggaggaagca gggctaactc aagcggagct tgcacaaaaa
     6481 gcacatacaa ctcaatctac tattgctcga attgagagag gggataacgt atctttcgat
     6541 aaacttgatg atatcgccag ggcattaggt aaaaagctgg tagtagaatt tgcttaaaag
     6601 cgagttgaaa atatatctat aataggctcc taactaatca attagtttag tgggcctttt
     6661 tttactagat agataaaggt tgtactatag tgtaaaagac aataaatttt atactaaaat
     6721 tactttaaag agaactagag agaaaggtac aataaatgaa aaccataaat cttataaaag
     6781 tatttatagc atcaccaggt gatatagcag caaaaaggaa tgaagtagaa gaaaccatat
     6841 atagatggaa tatagaaaat acagataata caaaagtgat tttgatgcct atacgatggg
     6901 aaactaatgc gattcctact tatgtaaata atagctctgg gcaaagtgtt attaataaac
     6961 aaattgtaga aaatagggtt ctgctgcaaa gttttaaaaa taaaatcagt ttttgtatta
     7021 tttcttcatt ttaagctgga attcccaata atatcctgat ttcagtgcag actgaaaagc
     7081 cgaagagagt gccttctttt cggcttttct tgtagattcc tcgaatggtt tccatccctt
     7141 taattgtggt ggaagctgta cgtagacttt gatacaattt atgtcgacgt tttatcgatc
     7201 gatg
//
LOCUS       DACJGB010000107         7173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.111, whole genome shotgun sequence.
ACCESSION   DACJGB010000107 DACJGB010000000
VERSION     DACJGB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.111"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(1145..1543)
                     /locus_tag="IYI34_002730"
     CDS             complement(1145..1543)
                     /locus_tag="IYI34_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560554.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIKFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1759..2814
                     /locus_tag="IYI34_002731"
     CDS             1759..2814
                     /locus_tag="IYI34_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAR0560555.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQDI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDESLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2834..3187
                     /locus_tag="IYI34_002732"
     CDS             2834..3187
                     /locus_tag="IYI34_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560556.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3369..3665)
                     /locus_tag="IYI34_002733"
     CDS             complement(3369..3665)
                     /locus_tag="IYI34_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560557.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3669..4487)
                     /locus_tag="IYI34_002734"
     CDS             complement(3669..4487)
                     /locus_tag="IYI34_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0560558.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4713..4934
                     /locus_tag="IYI34_002735"
     CDS             4713..4934
                     /locus_tag="IYI34_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAR0560559.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4936..5202
                     /locus_tag="IYI34_002736"
     CDS             4936..5202
                     /locus_tag="IYI34_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR0560560.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5315..5854
                     /locus_tag="IYI34_002737"
     CDS             5315..5854
                     /locus_tag="IYI34_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560561.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
     gene            6063..6245
                     /locus_tag="IYI34_002738"
     CDS             6063..6245
                     /locus_tag="IYI34_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAR0560562.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            6348..6632
                     /locus_tag="IYI34_002739"
     CDS             6348..6632
                     /locus_tag="IYI34_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560563.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6648..6884
                     /locus_tag="IYI34_002740"
     CDS             6648..6884
                     /locus_tag="IYI34_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560564.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6906..7160
                     /locus_tag="IYI34_002741"
     CDS             6906..7160
                     /locus_tag="IYI34_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560565.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
BASE COUNT         2491 a         1187 c         1106 g         2389 t
ORIGIN      
        1 cgtgttaagt ttctttacat tcctacgcac cttgcgtgta aaatttcttt attcctccca
       61 cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa ccgaacaagt
      121 ttttacctat tcccaaaatt aatttattcg ttattttttc attgattaag aataattatt
      181 tgatgcttaa attagctaaa aaaacgattt tttactgata taatcatgat attaaagctg
      241 aattaggcgg aaagataaaa tgttcgcaaa atcaaagtat caattatctt gtttttttat
      301 aaatttgtaa gtaaaaatct aaaaagtttt cttttaattg gtatatgtat taaaatcaca
      361 ttcaatcact cataaaagta tgcgctttca ttttttttgg atgagattaa tatcaataaa
      421 taacgatgta aaaaccatta attatgctct tttataagga tattaattaa tctaatttga
      481 aaaaataatt caaaatttaa atgttttaat attacaaata aaatcatgtt atcgtaaaat
      541 ggtataaaaa agctaaatat tctatttttg ttattttatt agcagggaaa tttaaatagt
      601 ttacaagaat ccgtagaaat atattggttt tgccttcaga ttaatactgg atcatattaa
      661 tctgaatata atatatgaac taataaatag cttaacctcg ctattttgtt ttttctgatc
      721 actatctata cagcattagt aaatagaatt ttaaaaataa aaaaattaag acaaaaaaag
      781 tatcaacata aacatataca tatacacgtt tatgttgata cacttaattt gtcctttaaa
      841 aagcacttat tttaatatta tgttatttta taatcttatt acaagctgat tgttagacgt
      901 tgtcctttca ttacatttat taacggttat tttatcgttt aatttattaa ttatttttga
      961 tctaaaactt agcttaacaa ataaataatt aaccaaccgc cagataaaaa tgcgatcatt
     1021 gctaatacat aagcaaagat aaaaatatgt tcgatcagct taaaccctga tgcgattaag
     1081 cagaccaagt aaaatagaaa ttctcttaca gataatggta attttctaac tagcatactc
     1141 ataattaaca ccccaaagta atattgtatt ttgaacattt tacatccaat acagcatcaa
     1201 tcaaccattg ccaatcaaaa atacaaggct tcattgaagt atgtttttca gatgaaaaga
     1261 tataatataa tttcttcgga ataatatttt cagttgttgg gatcaagcta aatttaatac
     1321 catctaactc atagctacca gctttctctc cattaaaaat ttccttagca ctttcttcta
     1381 aagaatcaaa atatttttcg acagcaacct ttgaagcttt tactattgaa gcatttccct
     1441 tttcgataaa atctgctttt gttttaccga caataagtaa ttcttgatca gtaaaatctt
     1501 ctaaaataat agtagtgtaa ttcttatttc ttctcaatcg catcaggatc actccttaat
     1561 ataatttatg cttttataat atcataataa gataaatttg caagtgttta tttacacctt
     1621 tttatttaca ctttattgta attgattata cttttttgat tgcatttttt tcttataaac
     1681 agttgcaact attagtaaat aggtgtaaac tattttcgta ccgaaatacc ataaacacaa
     1741 acagaaagag gtcataccat gaaagaacca ttattgtttt ccgttgactt agggaataaa
     1801 tcagtaaaag aaatgagtac acacgtttct ccactctcgc ttccctctcg tattttgaac
     1861 aaaaatatca ttaattcagg acgtttttct aatataggtt cctcattaaa acgatcacta
     1921 aacgtttctg aatatagcct agtaaacact actgaaagct attattttgg gaaggatata
     1981 cacgagctag gaaaagatga ctacgtgata gaatcactag gttttgggat cagcagatac
     2041 aagcaggaaa cctaccaaga tatcctatct ttctctttag ctaacctagc acgtttagaa
     2101 gatcaatctg atgacgtttt aaccgttaat ctagttttag gtctgccttc atccgactat
     2161 accgaaacaa tcgtcaaata cgtgatgcaa caagccacac gacaacattc agttgaagtt
     2221 gacggtaaag cttataatat aaacgtagat accgtttggg tactgccaca gccgattgga
     2281 acactatata acctcctcct tgctgatgat ggtagtgtac aagatgaatc attgatcagt
     2341 cagaagatcg gttttgttga tattggtggt ggcacgaaac tttttgatgt tgtccaaaat
     2401 tttgaaattg acgaaaacaa ccgtgaacaa aaggataccg gtatttttac tttatataat
     2461 cgtatcgtaa atatgcttga ccttccgatc agtcctaatg ctttccaact tgaaaagatt
     2521 atccgagaag gcaaagaaaa aggcgtatat ctatttaaac caagcggaaa tgaaagtttt
     2581 gatatttcag aagtagttga aagagaaata aaaacataca ccaatatggt tgttgcagaa
     2641 gttttagcta cattcaaaaa cttcacaacc ttagataaag ttgtctttac tggtggtggg
     2701 gcttctatta tcgacaaaca acttgtaaaa gataaaattc cgcatacctt gtttgttgaa
     2761 gacagtgaat tttctaatgt aaaaggcttt tacaagtttg gtttaactca ataaaacagc
     2821 gaaggagatc aacatgaata agggaaaatc agagcgttat gtagttataa tcaatagaga
     2881 aaaggttagc gagttacctt tagcagagtt tcttgaatct gaatatgaaa cactaggcgt
     2941 taaatactct cagacgatca aaaaagcttt gaatctatat cggcgtgtaa agaacaatga
     3001 agttacaatt attgaagcag gcaacgatag aaacgatagc aaagtagctg aagtaaagaa
     3061 taaaccaact gatcataacc ctattcaaca agcgcatcct tcaacggtta catccgatca
     3121 ttcagcaaag aaagaaaagc caatgctaaa agggttcaat gcaccaagta acaaagactt
     3181 actttaaaaa taaaaaggcg aggttacaat acctagcctt tttattatgt acaaatacaa
     3241 acataaacat atacgtatac atgtatataa acatgtatac gtatatatcc ataaacacgt
     3301 atacgaaatt tagttaagct atacctaacc gcttattcca ataaaaaaag tgatcagctt
     3361 taaactgatc actcaccaaa aacttcatca tacaagttat ctatcatttc accaagtaaa
     3421 gctgaatctg atcgaattcc taacttttcg ctgattttag atagtttctt tctttgtgat
     3481 ggttgaagcg tgaattgata atttttcttc ttttcagaac tatcactttt tagtttagct
     3541 accaaagaac ttgttgtttc tttaggtgtt tgtttaggtt cttcaactgc ttcaaccact
     3601 tcaggaactt cttctacttc agcttcagcg aattttttaa atctattttc tttcacttca
     3661 atcactcctt attcttgtgc cgtatcaatt attttgttgt atagcgtaaa cgtgctttca
     3721 aagaaatcct tattcttacg atagatattc ttatccttag cgaactcagc aatattctta
     3781 ccttctaagc tagttgcatt aagcaattcc ttctcttgta catatccaag ataattagat
     3841 aacgttggta ccaattctag gaactcacga cttgaattag tattatgttt gatcctgcta
     3901 ccgatcgtag cgtatttagt ttccatcagt gtttcaccag tcttaatatc aatcaactgt
     3961 tttgctagtg catcaatatc tttaccaagc ttaagcaaag cttcaaaacc atctttagat
     4021 ggatcagaaa tgcctacgac aagatcagat acagcccaag cattttgagc tagtagacct
     4081 gcatcattat gagtatcaat cagaattaga tcatacttct ctttaagctg ttcatagtta
     4141 acaacgatcc atctaaacag tagggttctg ttgttaggca tatcacgcat ttgcatctca
     4201 acttgtttta aatcttcacc tgctactaaa atatccatgt tctcatttat agctaaaggt
     4261 tcagctgatc catgaagata taaatgattg attgtattct ctcttttcgc tttactgaaa
     4321 gcatcaggaa atcgttttga taggttttgc gacttatctt catcaatata cagcaccttc
     4381 atacctaaat tttcgacagc atatccacct agattgtaga ttgttgttgt tttacctgtt
     4441 ccaccttttt caatgtttcc agtaatgatt gacaacttct tttccatgat taaacctccc
     4501 ttattaatct tttgtaactc cattatacac tataaacacg tttatgtata tatttatata
     4561 cataaacgtg tttatattta tatttgcttt tatattctac aacataaaca tatatatgta
     4621 tatgtttatg tttatgtata tattcaaata tataactatt gcatttgtca tacaatcgta
     4681 gtacaatgta attgaaaagg agttgataaa ctatgccagt aataagtatt cgtgtatcta
     4741 aagaagaaaa agattttctt gaaaaagttg ctgaattcaa cggagataac atttctgaat
     4801 ttatgcgtag taattccctg caaacagcag aaacgttagt agactttgcc acctacaagc
     4861 aactaatgaa agagcatgag gaaaacgatc aaagtatttc ccacgaagat atgctaaaag
     4921 agttaggctt ataagatgac ttaccaagta agatatgaac gtgaagctca aaaagcgcta
     4981 aaaaaaatgg ataagtttca ggcaaaggtt atcctaaact ggatcgagaa aaatcttgta
     5041 ggcactgatg atccaagaaa gcatggtaaa ggtctaacag ctaataaatc aggctactgg
     5101 cgatataggg taggtgcata ccgtataata gctgatatta gcgagcatga agtgacaatt
     5161 cttatattgt ctattggtca cagaagtgaa gtttataagt aacagacaag ctgataaggc
     5221 ttgtctgttt ttttgttaac ccaacaaaat cactgtttac gatcccactt tacctaaaaa
     5281 agaacaatat cctcttgata ttatacacac cagtgtgtat aatataagtg taaacaagct
     5341 aattaacttt caaaaggagc gattttttat gggatggtat ggtgattatt catcagcaaa
     5401 tgaagtgatc gaagaaaaga aagggtacgc caaaaatggt ttggaaatcg taggtaccaa
     5461 agtaacgaag acctatggaa ttatcctttt caaagatgca gaaggaacct acgatattga
     5521 cagcttcatc tttcgagatg aaatgtacaa accaatctac ttcttaaatg acagtcctgc
     5581 ttactttaag aaaattccga aaaaatggtt gaaattcttt gtcggtgatc aagaaaaaat
     5641 ggttaagcta caactagata gaattaaaga agaaagcaat aaaccaaaac ttgatgactt
     5701 attagttgca ggtaaaactt actcaatctg ggatggatcg tatgtcgctg tctatcagta
     5761 taaactcaaa cgtagcttta tttttcgact acaagatgga tcactcactc ggttcactcg
     5821 actatcagca ggagatgtta aggaagtaac ctaaaactta aatacataaa cataaacgtg
     5881 tatacgttta tgtttatgta tatgtatatg aatataaacg tattaggtac gaacactttt
     5941 aaattctttt ctgggctttg tttttagggt agggctaaaa aaaacatcac catctgatcc
     6001 aaaacagctc agaaacgcat ttaaacgtat ctgataccaa tattacagaa ggagtgtatc
     6061 gtatgattaa cctatttctt ttagtgacag ctagtacatt attctttact ggaatatcac
     6121 tgataatttc ctatttgatc tataagaaag ccgaaaatca cacaagcaaa gcaatcggac
     6181 tagccgtttc atccttttta actttgctga ctttatttgt tactttatcg ttttcttttg
     6241 tttagaacag ctgatccatt agcacctttt tctctttaca ttatacacac tgttgtgtat
     6301 aatgtatttg taaacaagct aattaacttt taggaaggag tgacaagatg ctaacagcta
     6361 ctttacagca acctactcac gttgctattg cagaaagtta tacaattaat gcagggatcg
     6421 ttaaacagct gatcaaggat cgagaattca aaaagtttaa cagtcggcaa gcgtataaca
     6481 gacagctaaa gaaagctatt ctaaagcaca aagattcctt catcgggaac ttggaaagtg
     6541 tagatgttaa aactgtttca acggcatttt cagttgttga agtgactatt gacggtcgct
     6601 tgatttctct atttgctttt ggaattaatt gatagggagt gtgaactttg gaaggtaaga
     6661 cttttgcaga aatttggact gaatttattg aggtaccaac tcgcattgat ccgataactt
     6721 ctgaaaaggt attggatgca gacttctaca atctcaaagc aggtactagc cttgaatgcg
     6781 tttggatgta tttagaagag catttcaaag gcgtaacgct cggtcacgtc ttataccaca
     6841 atggtgccga gtgggatgca attggaaata tccatttttc ttaatataag aaaggagaac
     6901 acaatatgaa ttataccgat gaagaacaaa tcgctttgga atacgttttc acaaatgaag
     6961 aaggggagga aattgaaaaa gtcttgatca tttcatcaaa agacttggag aaggctttgg
     7021 cttatgaacc atacgaatca accgtaactg tcattgaatt cttagatacc tatacttcta
     7081 atgatacttg ggaaattatg aatatttgca acgctaataa cattcaattt gctgaaaaaa
     7141 ccaataaatc attttattag ttttgcaata aaa
//
LOCUS       DACJGB010000108         7082 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.112, whole genome shotgun sequence.
ACCESSION   DACJGB010000108 DACJGB010000000
VERSION     DACJGB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.112"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            933..1607
                     /locus_tag="IYI34_002742"
     CDS             933..1607
                     /locus_tag="IYI34_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAR0560566.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            1604..1945
                     /locus_tag="IYI34_002743"
     CDS             1604..1945
                     /locus_tag="IYI34_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560567.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            1975..2334
                     /locus_tag="IYI34_002744"
     CDS             1975..2334
                     /locus_tag="IYI34_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAR0560568.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            2538..2717
                     /locus_tag="IYI34_002745"
                     /pseudo
     CDS             2538..2717
                     /locus_tag="IYI34_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            2859..4943
                     /gene="cadA"
                     /locus_tag="IYI34_002746"
     CDS             2859..4943
                     /gene="cadA"
                     /locus_tag="IYI34_002746"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAR0560569.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            5234..6700
                     /locus_tag="IYI34_002747"
     CDS             5234..6700
                     /locus_tag="IYI34_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0560570.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2341 a         1155 c         1516 g         2070 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag tgccttgacc tgagtattag agaaacaaaa cggaaaaata
       61 gcttctcata ttttaccgtt tttttagcta atatcgaggg tcagctgatt gaacaccgtt
      121 tatatcaaga ggttgtgcgc ctgccgttca gctccgagtc acaaggagta atttcaaaaa
      181 acagtttttc atgtttgatg actattgtga cttgtgaccg aggagttggc cggagagcaa
      241 cgtttattta aaaaaaagaa ctgcgaactc tcgcagttct ttttttaaaa tacgggataa
      301 aggaagtttc cagcagacaa gagaagaaag caaaagaaat tgctatttga ttcacaaggt
      361 tctataacga aatcgcaatc aatcaaacat atacaaagaa ggttattcaa atggaaatct
      421 gatttgatga tgcttctgct gttgaaataa agcagcatct agtacctgaa aacaaactaa
      481 tagtaacatt cgaagatgga gtaggtccgt attttcagca tgcaatgatc catatgtaag
      541 tttaattcat ccttgaaatc atcggaccag ataaagttat gaccaatcta tcccaagcaa
      601 tctagaagag atattagtaa aagaacattt actggaagtc ttagatgagc ctataaatat
      661 atgttctgat ggcgtcatca gtggtctgat tgacggaagc ttaggtgtga tcgatttctc
      721 tggaccaaac aacgaaggaa tcaaggggaa tccggcacgt taagaaaaaa agtataaaaa
      781 actagacact tgatgagcgt tcattcaatt gattgaaggg actaggctgg tgtcattttt
      841 tatacatata ttttctcttt cgtcttgata gggcttatgg gataatataa ggaagaaaat
      901 catcgtttta ttcaaaaagg agtttttgac aaatgaaaaa acgtatatta tttatcggtg
      961 acagcattac agacgcagga agagaccgag cagaagcaac tgatctaggt aaaggttatc
     1021 cacttttgat tgccggaaaa ctaatctccc aatatccaac gatgaaattt gaatgcatga
     1081 accgtgggat aggaggagac aaaattgcag atctgcaagt acgctggcaa gaagattgtt
     1141 tagctttaca acctgactat gtatcgattt tgatcggaat caatgatacc tggcataata
     1201 ctagcgatca ggctatattc ggaactgaga aatctgcgca gcaatttgaa tccgtttatc
     1261 gaaaattact aaatgaactt caaacagcgg aaatctgcaa catcatcttg atggagccgt
     1321 ttgtacttcc tataaccgac gaccgtcatg gctggcgtaa agacttagac cctaaaatcc
     1381 aaattgttcg taaacttgct aaggaatatg gcgcattgct ggttccttta gatggcatat
     1441 tcaatgcttt agggattgct tatggttttt cgaaatatac aagagaagat ggggtccacc
     1501 caacgattgg cggacatgag atcattgcac aaagctggat agatgcaatt gccgcagtcg
     1561 atttttttag tggagataaa aatgagcgaa agaaaagtga gaaatgatgg aaatacggtg
     1621 ggcagtaaaa gaagatttac ctcaaatcaa tcagctgatt actgatcatc cagaatcatt
     1681 cgtaaaagca aaaacggcag ctgacctaaa tgataagaat aaactattgc tcgttgctgt
     1741 aacaaaagaa aaacggatta tcggatttgc gcaagcggac gtgcaacagt cagaagaacg
     1801 ggtggatcag cgaccatcag gaaaactaat ccaacacaca ttagtaacaa aagaaaatac
     1861 ggaagaaatc caaaaaaaat ttgcagaata cttgatgctg gaagctaaaa aaagaagaat
     1921 aaaatcaatt tttgcccagt tttaatcaaa aacagtaata gaaggagaaa tacaatgagt
     1981 tgtttaggaa atgtcatctg gtttatattc ggcggactaa tcggtggtct tagctggtta
     2041 gcagcgggga tactttggtg tatcacgatc ataggtatcc caatcggtat gcaatgtttt
     2101 aagatcgcct ccctttcttt tgcgcctttt ggcaaagaag taatttatca ccagtctggc
     2161 atgtcattga tcgttaatat catctggctg atcgtttcag gcctaccaat tgctctcgca
     2221 catgtcgtca gcggcatcct tctatgtatc acgatcatag ggataccttt tgccaagcaa
     2281 tctttcaaac ttgctcgatt ggcgttgatg ccattcggtg cagaaattgt ataaaaagag
     2341 aataataaag gttagaaaac gcatagagtc aggtgggata catgtatttc acctgactct
     2401 atgtgttttc ctgtataagt tctcccatgt gacttttacg tcttttattt atatgggatt
     2461 actgtttatg gaaagttgaa ataattttca ttaaagaact ctttctatat ttttgaaagc
     2521 gtagtggagc ttcgaatatc tgggaaggac attttgcagg gattgaaaaa agatattgtc
     2581 ctatcgaaaa attaatgaat ttagacaaat tgccagcaac aggcttcaaa cttagttgct
     2641 atcctgtcaa gatcaagaag gcaagtgcgg gttggataag agcaggggca atgatcgaag
     2701 agaaaaagaa agaataagcc aaaacatctg tatggaagaa attttccgat gcagatgttt
     2761 ttcatttttt ctttcggata ccttgacata tgaataacca ttcatttata attagttatg
     2821 aagagatgtt catatgttga ggcgaggagg tcattaaaat gaaaagttac agattagaag
     2881 gtttagattg tgcgaactgt gcaatgaaaa tcgaaaaagg agttcagaag atcaatggag
     2941 tcaaggaagc aacggtaaac ttcacttctg gaaaactaac gattgacgca gaggaagatc
     3001 atttggctac gattgaacaa gaaacaaaaa aagtagtgaa ggaattagaa ccagatgtca
     3061 aagtaacaga aatagataag gaaaaagtat ctgaacatgg aaatgaaaaa gagagaaata
     3121 cgctgttccg gattttattt tcccttgcag gtatcgcttt attgctatta tttgatttta
     3181 atgaaccaat tcgcttgata gggtatttat tgatttatct gctgatagga tatgatgtag
     3241 tgaagaaggc agtgatgaat atcgtcaaag gaaaaatctt tgatgagaat tttcttatgt
     3301 ctgttgctac gattggcgct atgttgatcg gtgaatatcc agaagccgta gcagtgatgc
     3361 tattttatca aatcggcgag tatttccaag gattggcagt cagtcattca agaaaatcga
     3421 ttcgcgaatt gatggcgatc cgtccagaaa cagctcacgt gcagacgacg gaaggattga
     3481 tgaccgtgaa ccctgaagac gtgcagatcg gtcagtttgt acttgtgaag cctggtgaac
     3541 gtgtaccgtt agatggaacg atcatcgaag gagaatcatt agtcgatacg tcagcactta
     3601 caggagaatc agtccctaaa tctgtatatg ttggtgaaac agtattaagc ggatttatca
     3661 ataaaaataa accattttta gttcaagtag aaaaatctta tgaaaattct actatcagca
     3721 aattattaga attagtagaa aatgccagca gtaaaaaagc accagctgaa aatttcatta
     3781 ctaaattcgc gcgctattac acaccagtcg ttgtaggatt ggccgtttta ttagctgttt
     3841 tacctccact tgttgtttct ggagcggcgt tttctgaatg gatctatcga gcattgacat
     3901 ttttagtgat ctcatgtcca tgtgcattag tgatttccgt gccacttagt ttctttggcg
     3961 gtatcggagg cgcaagtaaa atcggtgtat tagtaaaagg ttcaaattac ttggagttgc
     4021 ttgcgcaaac cgagacagtg gtattcgata aaaccggcac attaacgaaa ggtgactttt
     4081 cgatccagac gatcgataca aaaatcgatc caaagaaatt catgcaatat attgcgagtg
     4141 cagaacagtt ttctacacat ccgattgctc aatctgtttt agaaggctac gcaggtccgc
     4201 ttttaccaac aactaatatc caggaatttg caggtgaagg gatcttagct gaagttgatg
     4261 gcaaacaagt attagcaggt aatcacaaat tgatggaaag atttgaaatc tcttttcctt
     4321 taagtcaaga aatcggtaca ttgctttatc tagcgatcga tcaaagttac agtggttatc
     4381 ttgttattgc agataccttg aaagaagatg ccgtagacgc attggttcaa ttaaaacagg
     4441 ctggtgtgaa gaacacagtc atgttgacgg gcgactcgaa aaaaattgct gatcatatcg
     4501 ggaagcaagt aggcgtggac aagatatata gcgaattgct tcctgaagat aaagtgcagc
     4561 ggttagagga aatcttacaa agaaacaata aaaagaccgc atttgtcgga gatggaatca
     4621 acgatgcacc ggtgctagca agagcagatg taggaatcgc aatgggtgga ttgggaagcg
     4681 atgctgcaat tgaagcagca gatgtagtca tcatgaatga tcaaccatcg aagattgcag
     4741 aagcaatcca tttagcaaag aaaacattga aaatcgtcaa acaaaacatt gtctttgcta
     4801 ttgggataaa aattttggta cttgccttag gtgcttttgg ttttgcatca atgggagatg
     4861 cagtatttgc agatgttggg gtgacagtat tagctgtttt gaatgccatg cgaagcttgc
     4921 atgtaaaaaa agaggacgaa tgaacgtcct ctttttttta tatccttttc ttaatgaaaa
     4981 atgtaaagaa aatagatagg attataaaat aactaatgtt tttaattttg cagaaaattc
     5041 aaaatagctt gaaattcaat aaggtcaatg aaataaaaga aaataactta tttttttgat
     5101 ggaaaaaata tgaaaaaacg atgtgtatta aataaaagaa tacacacata aaataacact
     5161 gtcttgtaag tttgttaaaa attgaatatg attacatctg gataacaaat ttttatcata
     5221 aaggagagaa cgaatgaaca acaaacaatt acgctggttt actgttgcgt taattgcatt
     5281 taacatggtt tggggcatgg ggaatgtagt taataactat gctcagcaag gagtatcagt
     5341 tgtcacctct tggctattga ttttggccat ttattttatt ccatacgcat tgatcgttgg
     5401 acaattagga tcagccttca aggatagtaa aggaggcgtc agttcatggg tcgaaaatac
     5461 gacatctaat aaacgactgg catactatgc cgcttggact tactgggtcg tccatattcc
     5521 ttatttggca caaaagcctc aagcaattct gattgctggt ggttgggcag tccaaggaaa
     5581 tggaaatatc gtaaatacga tgtcagtaca aatggtagca gttatttcac ttattatatt
     5641 tcttgccttt ttatatttat caacaaaagg actttctact ttgaaagtga ttggcggact
     5701 ggccggaaca gcgatgttcg tcatgtcgat cttatttatt cttttagctg taacggcacc
     5761 gttcatcaat ccaacgatgg aatttgccac accacatatg aatcaagtga aaacatatat
     5821 tccaacattt gatttcagtt atttcacaac cgtttcgatg ctagtatttg cagttggagg
     5881 ggcggaaaaa atatcccctt atgtcaactc tacaagaaat cctgcaaagg aatttcctaa
     5941 agggatgatc ttcttagcag caatggtagg gatttgtgct gtattaggct cagtcgcaat
     6001 gggtatgctg tttgctagtg ataatattcc taaagacttg atggcaaatg gggcatacag
     6061 tgcttttcaa attttaggta attactatgg tatcggtaac tcattgatga tcatttacgc
     6121 attgacaaac atgattggac aaatctccgc attagccttt tcaattgatg caccactaaa
     6181 aatcttgtta gcagatgcgg atccagaatt tgtaccaaac tggctacgta aacgtaatcg
     6241 taaaggtaca ttggtgaatg gttatcttct gacaggtata ctagtaggaa tcattatttt
     6301 gttgccgatt tttggtatca aagatatgaa tgaattagta aaatggttga cgaacttgaa
     6361 ctcagttgtc atgccaatgc gttacctatg ggtgttcttg gcatatatga tgttgaataa
     6421 agcttataag aaattcaatt ctgaatataa atttgttaaa aatccaaaaa tcggttttat
     6481 ctttggtgct tggtgctttg ccttcactgc gtttgcatgt atcctaggaa tggtgccaaa
     6541 agtagattat actgctgatc ctaaagcatg gtggttccaa ttatttacga atatcatcac
     6601 accaatcgtc ttgatcttat taggtatgat cttgccagca attgctcgtc gagacaaaaa
     6661 caaagaaatc actgaaccag aaacagtggt tcagccttaa aatagaaata cttgttacaa
     6721 gaggttgtga cagaagtggt cagcttcaag aaataagaag gaatttacga aaattgcttc
     6781 tcaaattttt gtgaagtttc ggcttgtttt tcgaagaagc tacttctgga acaccgtgaa
     6841 tcagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagc
     6901 ccctcatatt tttcgtttgg ctgggcttat taccgcagat gcaagctttg gaacaccgtt
     6961 taaggaagag gttgtaaaat cagcgatttg acctgagtat tagagaaaca aacggaaaaa
     7021 tagctcctca tattttgccg ctttttgagc taatgctgaa ggtcagctga ttgaacacca
     7081 tt
//
LOCUS       DACJGB010000109         7033 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.113, whole genome shotgun sequence.
ACCESSION   DACJGB010000109 DACJGB010000000
VERSION     DACJGB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.113"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(43..342)
                     /locus_tag="IYI34_002748"
     CDS             complement(43..342)
                     /locus_tag="IYI34_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAR0560571.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNEWFNRIRQKSPILYRWVMNAVRYEGYRW"
     gene            complement(961..1293)
                     /locus_tag="IYI34_002749"
     CDS             complement(961..1293)
                     /locus_tag="IYI34_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAR0560572.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2140..3180)
                     /locus_tag="IYI34_002750"
     CDS             complement(2140..3180)
                     /locus_tag="IYI34_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAR0560573.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
     gene            4992..5210
                     /locus_tag="IYI34_002751"
                     /pseudo
     CDS             4992..5210
                     /locus_tag="IYI34_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5470..5724
                     /locus_tag="IYI34_002752"
     CDS             5470..5724
                     /locus_tag="IYI34_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR0560574.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            5725..6042
                     /locus_tag="IYI34_002753"
     CDS             5725..6042
                     /locus_tag="IYI34_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR0560575.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            6601..>7033
                     /locus_tag="IYI34_002754"
     CDS             6601..>7033
                     /locus_tag="IYI34_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560576.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
BASE COUNT         2384 a         1128 c         1065 g         2456 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaaccattca ttaccttcat catcttcagg tagcaagccg aatccaattg gattatcagt
      181 caatttttga tagtataaat catcctcgtc taatgctaaa acatctaatc ctttacaatt
      241 tccgcctctt ctaaacgtca caaatccttt gtaatcccaa cgttggaaag aaaccttaat
      301 agtatgtgtg cccaatcgta tattcggatc ataatttttc atttgtcatt cctccattac
      361 gtaaatttca ttttctctat gttttatatc aattttttca ctttattcag gactcgttca
      421 tattttatcg tttttcgttt acttaaaaat acataaaaaa tatcgactcg tttatggcgt
      481 ccgaaacgag aaaccctagt agaaatcggt aaatgctcga ttgatccaat gaccaacgtc
      541 ctcgcaagta tctacatgta ctgcagcaca cgacacaatt tcttgtgcgt cctccccatt
      601 acgagaagac ctactcctta ttccatgagt ttgcgtcaaa gcctagatcc cttagtagct
      661 gctcatgatt aatctaggcg ctattctaac ttgtctaact tctaaattcc ctcttgttta
      721 ctctgtgcat attatttaca caaattcatt tcaccactat ccaaacaagg gcatttactc
      781 gattgaccaa ttttttacgt taccaatcgt aaaaacctac cccgtcaccc cccgaaagga
      841 attgttgata gatcaagtct actccttatt tttctagtaa ttctaagttc ttttagaagt
      901 ctcgacaata ttttcaagca agcgaaaaac gataaaatac aagttatgag aatgatcaat
      961 tcagctgatt aaacgcccca tcccttttgc ggtacaacgc aagactccat tttttcttcg
     1021 accaatcagt acaccgtttt ggtcataaac atgtttgatt gttggatata gttcttttaa
     1081 agggatagga tcttttaaaa ttatttgctt ttgtttacca gcgtaataaa tcatttcctc
     1141 tgagactttg tctttctgga ttatatattt cttacactgc acaacggttt tactttggcc
     1201 agttgatagt ttcttcgtga ttttctctgt agcggtacac cgtttaccgt agtgacaaat
     1261 atcttcgaga ctcaaaattc ctgtatatga cataaaataa tccacccact tactttaaat
     1321 atttttaata agtttttctt gttatttttc aaaaatagag aattgatttt cgttcatgac
     1381 aaacaaaaaa gagggaacgc atccaaacat gcgttccctc taatttcagc gttcgtcata
     1441 cggcaaagag ccacacggac atttttataa cattatgctt aggcatagat tcatcgtaat
     1501 atcacgtatt tttaacctta acacagccaa aaaaagtggt atcacttttg aatatctgtt
     1561 atactaaatc cgttgcgggt aataccgtaa cactgaaagg taaacgcatt aagggattaa
     1621 aggttgtctt aatgccacct gtggacgtcg acgaaggtta ccagccttcc gagtctcaca
     1681 gggcctttct tttttttgta ttttcaattg tccaaattta tcgtccaaaa gtgcagaacg
     1741 aaaattaatt catcacaagc gtagcagaca attaaattta aaacaactaa atttatacaa
     1801 aaaaatgtaa gcgaataata ttcgtattgc ctgaaaagaa gtaatatcaa ggtttttagc
     1861 ttttttaaaa aatgaaatta cctctcttcg cgtattatta taactaaaaa acagcaacat
     1921 gcaaacgttt gttctctttt ttgtgttgga aattaattat taactattaa cacttatgac
     1981 gtaaaaagct acgtttgtga tttttatcat aatattttaa ctcataaatt ctattaaaat
     2041 agctatttct ttgtaacatt tgtaatataa ttagaataat aaaaaatttt taattttaat
     2101 gctactccat gaagcaacaa tcaatttgtc agtgaaattt cactctaacc agtctttcgt
     2161 aatagttttt gggaagcttt cagaattcaa acgtttttca cttgatactt tagcagctat
     2221 tggtttttcg ataaaccaat tttttacata gacaaaaatc aggttctgga tattttcaac
     2281 tttttcggtt tttaattttt gataggcttt taacagtgtc gtgtataatt cttgttctgc
     2341 attgacataa taactatcga gttcttcaaa aattattgaa ataccacttt ctgtttcggc
     2401 ttttttcttt gcattatgaa tagtctggac agttttttga gcttcactgt aacttgaaga
     2461 aaatgttttt attaaattta atactcgttc agggataaac gtagcaatgc tgtcgtttgt
     2521 cataattgtc tcaaaattgt ctaacaacaa ttgatcttgt aggcggtctt tttcagtgtc
     2581 tatattgtgt ctattagtgt ctaatatatt attattaagt tctggtatga ttttcatacc
     2641 ctctgattta tcaacgtttt ggcacggtat gattttcata ccctcttgag ccccaaggga
     2701 ttcagcgtct gatttttcgt cgagggtggg tatgattttc ataccctctg atttatcaac
     2761 gttttcccta ttctctctat caaatttttc gagtaagtag atatcatttt ctgagacttt
     2821 taattctgct agatatagtc tattaggatt atttttccct aactgtttgt taaatcccat
     2881 ctgcttttgt aataataaac caaaggattc caactctttt ttgattgcta tcgctttttg
     2941 tttttggcag tttaatacgc tacatagttc atttgtcgta taagtaaaat aaacattacc
     3001 ttcttcatcg atttgccgtt tgctgattgc gatctctaat cttgctttta aaagcatata
     3061 ggcaatctta gttgaatctt tcattttttt gtattcgtca gaaacaataa agacttttgg
     3121 aaattgataa aataattcgt tgtaaactct atccgcctta aaaaaattaa aatcagtcat
     3181 tgtagatcct ctactttcac gttgttttat ttatgtaaaa ttacctactc ttttcgaaaa
     3241 tagcaaagat aaataaaaaa tgcaaacgca ttaaaactgt tttttaatta aatactacct
     3301 aaaatcagaa ataaaatgaa ttatgtacta cctttaaagt cattaagaac tactttaaat
     3361 aaatattatt tataattaaa tactaccaga agaacgtatg aataaaattt agttttaaaa
     3421 ttaaagataa ttaagtacta ccagtgtcaa aacaaaaaat aaattaagaa ctaccttatg
     3481 catataaaac atactaccat tactagtaat atatactacc ttatttcagt agtatatatt
     3541 atattcttct tataagacaa ggctttaggc tagaaaacaa tagattatat actactaaaa
     3601 aatacaataa gtactaccaa tgtcagatat atataataat tatgtactac cattaaaatt
     3661 aacttattta ataagatact actgtgttaa tctgaaaaaa attactttaa agtagttaca
     3721 tactacctgt taaattagat actaccatta atacttaaat tagatactac ctatatttat
     3781 attaagtact accgttctag gaataagata ataaaaaaaa actaccaaat tgcaattaaa
     3841 tactacttaa aatgtaaaaa ataagaacta cccaaattag gtagttctta ataagagaat
     3901 atcagataaa tttgtacaag tcataaaaaa attaatctta aatttttaaa tatatatacc
     3961 tgttaattat aaactacttt atttaggtag tacataatta acagataaaa ctacatgctt
     4021 gtagtttgtc tagctaatga gttgcatatg atttatataa aggtgtgcag cataacttag
     4081 ttgaagttaa gagaatagaa taaatatttt ttgttttgct aataattaaa cactacctaa
     4141 atatggtagt gtttaattat ataatattca ttttgttaca aataatcgta ttataaaagc
     4201 ataacaattg ggataactat tttttagatt atttaagtac tacttaaatt aggtagtact
     4261 taaattttgg tttaaggtag taggtaatta agaaataata tatatcttct tttataatag
     4321 aaggggagag gcctagttaa aaacagcgac aattaataac aatgtattta atgcttcaca
     4381 aaatccattt ggtggacaat gcattgttat tagcgatgat ctaccgttct aagtagtttt
     4441 ttctattggt tgcattcgtg actaattatg gtataattat gagtaacaaa gaggtatttc
     4501 attatatcat caaaccccat cagcgtgtag gctgatgggg ttttttttac gtttctatat
     4561 tatagaatta actggtaggc ttactgtctt ttttggcaat tttgcaatca agccacttgc
     4621 agatgtagtg tgcagttacc tctgcaaatg gcttgattgc aaaaacaaaa tcaaaaaggt
     4681 atttcattat atctcctcct ccccttgcca gattaggccg gggtagcaga cagcaaaact
     4741 attataacat aactgaaata cctaaatgac atatacagat atgataattt atgtattgat
     4801 ttttctaaca aaagcacata ttttaatata tactacctat tttaggtagt atatattttt
     4861 agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg ctacatagag
     4921 ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg
     4981 atgaaattgc tattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt
     5041 ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa
     5101 ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga
     5161 acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat
     5221 atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct acttaaatgg
     5281 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
     5341 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
     5401 acaccgttgg caattgtcgt tataacatgt tataatttat ttatcaaaaa ctaaaagaag
     5461 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
     5521 cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac
     5581 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
     5641 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
     5701 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc
     5761 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct ttctccagcc
     5821 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
     5881 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
     5941 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
     6001 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt
     6061 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
     6121 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
     6181 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
     6241 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     6301 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     6361 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt gttttaaaag
     6421 aggaagtcca atatatctat gggaaacttc ttaaatatta tgttggtaca tgaaaatata
     6481 catccctgct agactattta caacatttta ctttgtacgt attcttgttt taaaaggaat
     6541 gggataaaat ataggtacaa taactatttg atgctaaata taattaatgg aggagagata
     6601 gtgaacatac ttaatgaaaa aagatatgta tggtttgctg gtaaaaaata tattactact
     6661 ttcaaaagat tatccgagtt aatgcagcag actgttaaaa gtgctatgcc tgaaggatac
     6721 aagtttaatt ttgttcaatc ttcagtatct actgaaagta tctatttttc aattctttct
     6781 gagagtaggc tttttcattt tagaataagt tctcatgtaa agaaaactga tgttgatata
     6841 tatacgttcc gtacaaataa ttataaaaat ttaaatgatt tgagtagaag tatcagtgaa
     6901 tatctcaagg ggaatagcta tctcaagatt cgattttcac atatattaat tttactacca
     6961 tatttgaatg agaatttcac cctaattcaa gtagataagt ctttcattgc ctcaggtaat
     7021 caaggaaata tga
//
LOCUS       DACJGB010000110         6902 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.114, whole genome shotgun sequence.
ACCESSION   DACJGB010000110 DACJGB010000000
VERSION     DACJGB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.114"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..444
                     /locus_tag="IYI34_002755"
     CDS             <1..444
                     /locus_tag="IYI34_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAR0560577.1"
                     /translation="KEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRK
                     VEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKS
                     MTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            444..1643
                     /locus_tag="IYI34_002756"
     CDS             444..1643
                     /locus_tag="IYI34_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAR0560578.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1659..2303
                     /locus_tag="IYI34_002757"
     CDS             1659..2303
                     /locus_tag="IYI34_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560579.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2314..3120
                     /locus_tag="IYI34_002758"
     CDS             2314..3120
                     /locus_tag="IYI34_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560580.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3142..3372
                     /locus_tag="IYI34_002759"
     CDS             3142..3372
                     /locus_tag="IYI34_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560581.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3886..4194
                     /locus_tag="IYI34_002760"
     CDS             3886..4194
                     /locus_tag="IYI34_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560582.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4196..4618
                     /locus_tag="IYI34_002761"
     CDS             4196..4618
                     /locus_tag="IYI34_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560583.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4638..6227
                     /locus_tag="IYI34_002762"
     CDS             4638..6227
                     /locus_tag="IYI34_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560584.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6239..6574
                     /locus_tag="IYI34_002763"
     CDS             6239..6574
                     /locus_tag="IYI34_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560585.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6715..>6902
                     /locus_tag="IYI34_002764"
     CDS             6715..>6902
                     /locus_tag="IYI34_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560586.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2648 a         1036 c         1419 g         1799 t
ORIGIN      
        1 aaagaaaaac aaatggaaga acaagacttc aaacgcttct tggcgatgat tgatgagtgc
       61 cataatgcga ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga
      121 gaaatgcgta aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg
      181 ccagaaaata tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct
      241 acgaagatct ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt
      301 aaatcaatga cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg
      361 atcaattttg gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta
      421 aaattgtttg atggaggaaa ataatggaaa accaaaatga aagtttaatc aaacagtatg
      481 ttaagcgtcg tgcaaagcgg cgcttatttt tgtgggtgtt tggtacttct gcaggtttga
      541 ttactatatt gatcactgtg tttgtgaccc tttttctaat acttgcggca ggttctattg
      601 ataattctga ttcaagtagt ggtggcgaag cttttacagg agaatattcg gaaggcttac
      661 ccatttataa agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgctg
      721 taggtgcagc agtgaagtat aaattattac caagtgtgat cctttcgcaa tacggttatg
      781 aatcagcgtt tggtacgtcg gcgtccgcaa gaaatgatct caattatttt gggattacgt
      841 ggtttgatgg gtgtctgttt cctaaaggaa ctgcgcgtgg aattggtggc attgaaggtg
      901 gctggtacat gaagtttccc aactcaaaag ctgccttctc ctattacggc tttatggtag
      961 ccacacaatc aaattttaat gcttgtgtcg gaaataaaag ccctggcgca agcttattga
     1021 tcttaggacg aggagggtat gccgccgcag gaattactga agacagtgcc tattataaaa
     1081 attgtatgtc tattattaat acaaataaat tgatggagta cgatgagttt gcgattaaac
     1141 actggagaga aggcggcgga actggtggta cgattacagg aagctggggc aatccattcc
     1201 caggaagtag cttagataaa aattcgttca gtggaggaca actttttggg aaaaatccag
     1261 gaggagaatt ccgacctaac gggttccatg atggactaga ctttggttca gtcgatcatc
     1321 caggaagtga gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta
     1381 tttcaggttt gggtgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc
     1441 aagaatttgc aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg
     1501 gtcaagtaat tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt
     1561 ggcgtcaagc acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat
     1621 ttttgaatag cagcaaaaaa tagatcagga gaaaaaatat ggaaaataaa accttaaaaa
     1681 tcgtcttaac agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc
     1741 ttaaagaaga acaagagaca aatacctata aaattgaaga agtggctaaa aacgcgtctg
     1801 ataagctcaa aaaaaattct tctgaggcac aggcgaagat cgacaagtta aatcaaacca
     1861 tcaatgagaa aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag
     1921 aggaagtaac tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata
     1981 aatcattaag cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga
     2041 tcgacaaatt aagtcctgca gacgatgatc accaacatga cgactacgga agttactctt
     2101 ttacattagg tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt
     2161 ttgtcgtatt tgttgattat acaatcggca atcctcaatt taaagatgtc aaaccacaaa
     2221 aaataaaagg tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcacag
     2281 actttagtta tttcactcgt taggaggaag aaaatgagta agaaaaaagg agcaagacaa
     2341 cgttttaaaa aacgaaaaag ttatcttaga tggctgggga tctttatgat tctcggcttt
     2401 ttgattcttg ctagtccgat ttacacacat aaaaaggtag acataccaga tgaagtaagc
     2461 acgaacgcat ttttaaacac gaaggaactg acgatggttt caaaagaaaa aggagcgaac
     2521 cagcaattag tttttgaatt tgcgatcgac gatgaagatc agagcttatt aaaagaacta
     2581 gctaatctta attatcgtgt agaagtaaag acagctaaag gggattatca agcaataaaa
     2641 gccaaagtga tcaaggtgtc tgatgattat tttgtagtga agttagcaca tgtaccagaa
     2701 gaatatatcg ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg
     2761 caagaaccac aagatctgat ttattacgtt cacgaagata aggttaaaga tcaagtgaaa
     2821 gatgaagatt atgaacaaca tgcgatcaat tacaaagtga aaggatacaa aaaagaacaa
     2881 aaagcagcac aaaaacaaat ggaatccttc caagcaacca ttgatttgaa tgaagaatta
     2941 gtgaagaagc taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa
     3001 aacaagatta atagctacca gcaagaaatt gaaacatcga aaacgcaaat gaaagatcaa
     3061 gaagagatca ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag
     3121 aaggacaggt ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct
     3181 gagcagaaag tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga
     3241 gaagtgaatt tatctaatac aacaatatct gagttgaaga atggtcgaaa gaatttagat
     3301 actttaaaat tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct
     3361 gataacaaat aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga
     3421 atcagctata tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc
     3481 taacgcaaac atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca
     3541 accaaacagg aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca
     3601 tatgacaaaa tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac
     3661 acaaataacc ttaatttata aagagcataa ggaaacgtgg gaacactttt taaaaaaagt
     3721 gttccaatag gctgtgccta ttacgtttct caaaaataat tgtgggaaca aaatatttaa
     3781 aaataggata gtgaaaggat agcaaaggat agtaaaagga tagaagcagg atagcgaaaa
     3841 ggataggctc cttttcgcta tcctttttaa attggaggaa ttaatatgga agtaagaata
     3901 agaaatgtag atgagaaaat cgtaagagaa attgatcgaa gatgtgacga actaagtaac
     3961 cgaacgggta ataaatggag tcgtaacgat tatttaaagc tactcgttga aaatgatttt
     4021 gatcgtcctt tgatggaata taaacaagat aaatttgatc aattattaaa tcagttaata
     4081 gataaatttt cttttgttca aagtcataat acaaaagtat tagaagaata tatttgtcag
     4141 aataatcaaa ttatcgaact tttaattgaa caaagtagag gaggagaagt ttaacatggc
     4201 caaagttata gacaagcata tttattttta tgaaacacat atagatgttc tagatgaaat
     4261 tattgcaact catgcagagg ttaaaaacta tagtgaagcc gttcggtttc tatgtatgaa
     4321 ttatgattca gaagtggaga aaaaagtaaa ttccaatcaa gcaaagttga atgcaatggg
     4381 aaaagaaatt tctattttaa ccgaaatggt ttcagagttt gcggatctcc atttgaaaga
     4441 tacagggatt ttaattggta caaattcaac tgtttaccaa gaagcaaaag agcgtgtgga
     4501 aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga aaacaataag
     4561 cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggttcttaa aacgataaaa
     4621 attagaagga gaatgtgatg agtaaatcac ctgcagtcat tctcacttgt caatttacct
     4681 tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa aaagcgctgg
     4741 aagaaaagga acgacgtacg cccgaggaag aacaggaact gcgtctaatc aagaatgctt
     4801 tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg ggtaaagaat
     4861 tcagcgaaac gtcgaatgaa gcgcaatcgt tgatgaaaca tggaatcaat tttgacaagt
     4921 tagaagaaca agattttacc aaatatcttt cttatatgac acgtcactat gcgttagaac
     4981 aaaagaaagt actgacgcat agtgaacaaa aagaatacca acgcttgaac caagcaataa
     5041 aaaaatatca aacagagcca cacaaaaaag cttcaggaaa tcttcctgga gttttttcta
     5101 tggcttcaga cgaagtgaaa ggaagtgatc tgaaggagat caagcaaacc ttccaaagag
     5161 gacaacaaaa aggctcaatc atttatcaag atgttgtctc tcatgatcat tcgtatcttg
     5221 aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagaaggt ttaaaagcag
     5281 ctggaagaaa aatgatggag acattgttcg agaaagagca actgaatgaa acaggctact
     5341 ggatggcaac gattcaccga aataccaaac atatccatat tcattttgca gttgttgaaa
     5401 aaaaaaatac gcgtaaacca tacactgaga ttgaaaatgg tgtgagttat atcgtaccga
     5461 aagggaagag aaggcaagca acattagacg aaatgaagac taattatgta catgagttag
     5521 aacgtttttc ttatgaaaaa aatcgaatgg gtatggagaa acgaaattta ttgacacgga
     5581 aaagtgattt gcgtaacacc ttaaccaaag tagtgaaaga gccagtacgt tatgatcgac
     5641 acgcaatgag cttattaact gaagtatata aaaacttacc taaaaagcgt tcggaatggc
     5701 attacgggga tgaaaatcga accaaacttt caccatacac aagagagaaa ctcgatgagt
     5761 taacccaata tgtgttaaca aaagaacctg gatatcaaga atatatacag atttcaaaag
     5821 cattgaaaga agaagcaaaa agtctttatg gggaaagcaa gagagaatca aaggatgcag
     5881 aaaaaaatgc tctctttgat ttaaaaaagc gcacaggaaa tgccattcta acagaactga
     5941 agaaacagga tagtcaaata aaacctttaa tagaaaggat ggatcagatg caatctattt
     6001 acaatgaacg atcctttcga acacttaaac agttgccaac gaatgaatac aaatatagtt
     6061 acgaagaatt catgaagcgc aggaaacaat tccaacaaag agttgtcacg caacggagtt
     6121 tgcgtcaatt aaatcgaacg attaatgccc atgaagaaaa ataccacgca agtaaagcat
     6181 acgaagagca acaacgaaga attcaaatgg aacaagaaag acagtaggag gaatagcaat
     6241 gaaacaatat ttagatcaat ggaaagttat agaaggatca ttaagagaag aacgaatcga
     6301 acaacttccc gattgtttag aaaaagagca tttgtttcag ataagagaaa tgttgataaa
     6361 cgaacagttt gatccaaatc aatttttagt cgttgagtac ccagctactg gtgtgtactg
     6421 ttgtaatcat gtgaagggtg aaaaatattt tatcattcaa gaatatgagg gcaagttagc
     6481 gccttattat actacttggg aaatgaatga agaggggatc aataacttcc cgtgtaaaag
     6541 cattgaagag tctatttcac tcacagaatg ttagttgtta agtgaataaa agaagtaaaa
     6601 gagtcctgaa cgtgattgtc caggactctt ttttttcact gaattgtttt ccgccaattc
     6661 attgagtggt gcgatttccg tctacattat atcaaaagaa aggaggaaga agaaatggct
     6721 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atcttgtttt tgtgacagaa
     6781 attcaaacag gcagagcttt tgtagtcaca gacaaagcgg caaaagcata tcaaagtggc
     6841 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca
     6901 tt
//
LOCUS       DACJGB010000111         6840 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.115, whole genome shotgun sequence.
ACCESSION   DACJGB010000111 DACJGB010000000
VERSION     DACJGB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.115"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(105..395)
                     /locus_tag="IYI34_002765"
     CDS             complement(105..395)
                     /locus_tag="IYI34_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAR0560587.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(531..710)
                     /locus_tag="IYI34_002766"
     CDS             complement(531..710)
                     /locus_tag="IYI34_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560588.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(707..1057)
                     /locus_tag="IYI34_002767"
     CDS             complement(707..1057)
                     /locus_tag="IYI34_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560589.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(1050..2375)
                     /locus_tag="IYI34_002768"
     CDS             complement(1050..2375)
                     /locus_tag="IYI34_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAR0560590.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            2825..3439
                     /locus_tag="IYI34_002769"
     CDS             2825..3439
                     /locus_tag="IYI34_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560591.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(3604..4428)
                     /locus_tag="IYI34_002770"
     CDS             complement(3604..4428)
                     /locus_tag="IYI34_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560592.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(5066..5323)
                     /locus_tag="IYI34_002771"
     CDS             complement(5066..5323)
                     /locus_tag="IYI34_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAR0560593.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(5316..5585)
                     /locus_tag="IYI34_002772"
     CDS             complement(5316..5585)
                     /locus_tag="IYI34_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAR0560594.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(5716..5895)
                     /locus_tag="IYI34_002773"
     CDS             complement(5716..5895)
                     /locus_tag="IYI34_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560595.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(6276..>6840)
                     /locus_tag="IYI34_002774"
     CDS             complement(6276..>6840)
                     /locus_tag="IYI34_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560596.1"
                     /translation="EQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETEK
                     YIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDD
                     YYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYT
                     YDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         2098 a         1299 c          997 g         2446 t
ORIGIN      
        1 ttgttactaa taattccctt cttatagtgc attttcttag aaaaaaacaa tattacgata
       61 tcatgatatt aagatatcgt aatattatac aattgtgatt gatattatat atgttttttt
      121 atttttttat catgtttaat cttgaaggaa tcatattctt tttctaaaga caggagcttt
      181 tcataaacat cagacatgtt taaactagag gccaggctta aatcataaac gaatgaagca
      241 ggtaatgatt ttattctacg ttgtcgggtt atccagcttg caaccgttcc ttgtgggtag
      301 gcatataact cacaaaattg ttctacagtg attcctaaat tcttgataat gtaaacattg
      361 atcgggtgcg agtaaacaaa tgtattgata gacatggtgc gtaagcctac tttcttttat
      421 tattgcgttt gcaataatag tttaacacac gcattattct agtatctact cctatttgta
      481 aaaacaaaaa aggattcata gagataactc tatagaatcc gtaaaatacc ttatatatat
      541 aacgggaaga tataaaacct aaaattatga taacctgctg cagcttctac atagaaagag
      601 aagcgctcaa caccaacctc atttatcaaa cgttggccat aaccccaaat ctcttctgat
      661 agctgatcaa aggttatata ctttagctca tattgctgga taatgttcat tctaatcgac
      721 tccatttatt atcctggtaa aattcgatat gacgtatttc gtcgtagtga attttgtctt
      781 ttccaacgta gatccccagc tcatcactac ctaagatctt gccaatgata tcttctggat
      841 agtatccttc agcgtttcgt tcttcttttt gaatagatac tgatctattt tttatgactg
      901 cagcgttcaa tacttccgca atctcttcag tcgtcatttg ttcttttgct aaattttgac
      961 tggttcgttg ttcattgtcg gcatcaattt cttcagtatg atcagataaa taaaaccctg
     1021 cccactttaa acgtccacga tcattgtaat caatcatttt taattccccc atttccgccc
     1081 gcatgaccac caacaagtgt gcttctagta atgcttctag cgccttctaa cattgaagaa
     1141 gcatgtacaa tcgacgtaaa accatatttt tgccgaatct tgtcaataat tttatctaaa
     1201 ttttcttcgt caatttgctt ttgtggatca ctaaataaat ccagctgaag cgaatcggtg
     1261 tacacaagct tgctataggt tattccaaca tgccttactt cttgtccttc ataatactta
     1321 tcaaacaaga aaagacaata attctgcaat tcctttgtat tatctgtgat agggatcttc
     1381 atctggtgtg aaaaccctgg tctcgtttct aagatcgacg tgccaatgtg taaatggaca
     1441 caacctgttt tacagttatg ctgcctaatg cgtatcgcta cttgttccgc catttctttt
     1501 accaccagct cgatttcatt tcttctagta taatctcgtg gtaacacttg tgaatttcca
     1561 tatgattttt cctttgtcgg ttctggtggg attgctatat ctgttcgatc gatcccatta
     1621 gcatggaagt acagctgtaa gccaatcaca cctagtctat tcttaatcgt ataaggattc
     1681 cagttagcaa gatctctaat gctcataatc cccatctgat tgagtctttt tttcattcga
     1741 gagccaattc cccaaaagtc cgtcatttca ggaatattcc atactttttc aggaacgttt
     1801 tcgtacgtcc attccgcaac aaatccttca ttatgctttg cttcgttgtc taaggctagt
     1861 ttagccaaaa ggggattatc tccgacacct actgtagcaa tcaatcctag ctcttcttta
     1921 atattctcct ggatcatttg agccagcttc tttctccgtt gacttcgtgt tccttcagtc
     1981 gtaaaaagat tcagtgatcg ggtcactttt aagattgatt catcgatcga gtaaatcagt
     2041 agatcttcat cagccacata tcttctgaaa atattattta cctgcatatt tcgcttgata
     2101 tataggttca tacgtggtgg aacaacgtgt agtgttttag gaaatggttg tggtaagtca
     2161 cgtggtctac tcacatttgt aataccatac cgcttttttg cttcaggaga agaagctaat
     2221 atcaatcctg aaccagtatt atcagctcga ctcataacaa caagttctgt tgttaatgga
     2281 tctaaatttc tttctataca ttcgacactt gcgtaaaagc ttttcatatc gattaaaaag
     2341 taatcattta ctggctcttt tgaataatcc atcataaata acactccatt ctttcacatt
     2401 atacaaacat atgttcgata ttgtaaagaa gaaatagcta aatacataaa aaaagaagca
     2461 taggtatgct atcaccctgc ttcttctttt gatttgtatg atcaatttat tctatcactt
     2521 tactaattaa tttgaaaatc taataaaaac tctcactcta aaagagtgag agactgcaaa
     2581 ttagagtacg gacttttttg gagttccgtt ctttaatatt aacaaatgaa taaaatcctt
     2641 acaacatttg agaataagtt atcagtagac acttacaaaa aattctcata ctatttcctc
     2701 catcattagt ttttgtctat ataaggttta ccctaaatag acactggtat aacattgaaa
     2761 aatatggctt cctcgtgtat gattagggtg tattttaaat agttgcagaa agtaggtaaa
     2821 agttttgaga gttgcataca tacgtgtctc atcgatcgat caaaatgaac aacgccagat
     2881 tgaagaaatg aaaaaattcg gtgcagagag aattttcatc gagaaacaat ctggcgcaac
     2941 gattactcat cgacctgttt tccaagaagc attagattac gtccgagatc aagatatttt
     3001 tattgtggaa gctattgatc gacttggaag aaattatgac gagatcatcg cttcagtcaa
     3061 ttacttgaag aagaaaaatg tgaagctgat cattactagt ttaccgatca tggctgaagc
     3121 aattggcaat cctctcctgg ataaattcat taaggatctg atcatccaaa ttttggccat
     3181 gatcgctgaa caagaacgga ctgaatcgaa acgtcgccag gcacaaggaa ttaagatcgc
     3241 aaaagcaaac ggcgtctaca aagggcggcc caaattatac agtgcagacg caaaagatcc
     3301 ccaacgtcgc cttgtttaca aaagcatcgt tgaagatctt aaaaatggtg ttgcgattgc
     3361 taagattgct aaagactaca atgttacgag acaaaccgtg tatagaataa aaaaagacag
     3421 catggtcaat gacaaatagt tgatcatgct gcttttatta tcttctcatc tttaatttcc
     3481 tacatataat tactatctat aaaattaagt cttactcaga acagataaag cataaaattc
     3541 caacaaacca ttttttactt aactaaataa agagtctgtt aatataatct tcacttattt
     3601 acgttaatcc cctactgtat ttgttggttc cttatctata ttttctacaa aatccttatt
     3661 atcttgtgat ttagcttcgt ataattcttt gtatgtcgac acattggtta tactaattcc
     3721 tatagctagt tcttgagact ctacttttct ctctagctcc tttgacaaag tgatttgaga
     3781 tttatttggt tccgttggag tcaattctat agattcttca taaaaatctc tatacataaa
     3841 gctatcatta attttataaa aaaaattaaa tgtttttagc actgccatat aacattcaaa
     3901 acattcttct atagttggtt tacttccttg aggagaatga accaatttat tacgtttttc
     3961 tatgaaattt ttaatttcct ttcttatttc tttcggaaac tcaatattaa agtacccttt
     4021 aaacatgctc cctaataatt tgtctacagg gggagattgt aaattctcaa atattagagt
     4081 taaatcaggt ttctcttgtt gataaaattc ctttacccct atttcacagg ctgtaattat
     4141 cgaaataaac ctatctctag tatttgatat atttttactt cttttaagga gtataagaga
     4201 aaaggggata ctttcgtgtc catgcatgta attaactaat tgttgagctt cagaagttat
     4261 atctctaatt atatagttaa cggaattagg aagtgtcttt aactctaatg aaatttctat
     4321 ccaattacta tcttctaatc tgtatatgct cgaagtataa tttgattcgt cagcaagcat
     4381 cgctacatcc caaacactga tcataaaatc ttttaaacgt tcaatcattt cagcaatatt
     4441 gctaaaatca gctaattttt ctttagaata aaaaaaaaca tttatatcag aatcaataaa
     4501 ttctattttt ttattactat atcgtcatca ttatcttgac catttatgtt tattgttccc
     4561 tgagtgggga gtacttcttt caaattcaag ttaaatttat attccattac ttctcctcca
     4621 atatataaaa attacttaac tgtatccaaa tttaatatgt aaataaatca ttaattttta
     4681 gctaaattct atgagaagtt tatttttgtt gtttttgcta attaactaaa actagtacaa
     4741 aaaatttaaa atagattatt aaacctattg cttattctat tcagtcatat atataagcgt
     4801 catcctttag taactaacat cccacactac aatagtatta taacatatct attcaacttc
     4861 aaattgttga aatttaagcc atttatattt acttttcctc aactttagaa atgattttac
     4921 taaccttata tccgtaatac gcgtaacaaa cacattcatt aataacaatc aacactaagc
     4981 tatattagct ttttaaatga ctagggtaaa taaaataagg atcaaaaact tttaagtttc
     5041 tgaccctttc cttacttccg attggttaat agtgatcttt tgcagaataa ataaatatcg
     5101 tttcattttc aactctatat atcagtctat gttcatctgt aattcttctg gaccattttc
     5161 cagataaatc atgctttaat ggctcaggtt ttcctaatcc agcaaagggg gaacgatcga
     5221 tatcttttat taacttgtta atctttttta tattgctttt gtttccttgc tcatgccaat
     5281 aaagataatc atcccaagca tcatcagacc aagccttaat catcagattc aacctcgatt
     5341 aagtcatgtg ttttaaatgc acctttggag aattgttcat ctcctcgacg aattttttcc
     5401 atgacgtaat tattagaaag tgttctcaac gtttcttgca tagaatcata atctcttttt
     5461 gataatacaa caactgtatc ttctacatct ttacttgtta caataagtgt ttcagcatcc
     5521 tcattaactt gtttcatata actacgtaaa ttttggcgga aatttgaata agctactgct
     5581 tccattcctt tcacccctta tttatttcta taaaacaatt gtacattata ttgtacaatt
     5641 aagcaaatga ttaatattct aacttcttat aattaaaagt gctatcactt ttagttttta
     5701 ggataaaaag tgacgtcact ttttgagtgc tggtacctca aatcctgctt tttcttgggc
     5761 aatcaataaa tctaacgcaa tacacataat cctggattca cttttgccgt acttctgctt
     5821 tagttgctgc aattcttttt gtctcttttc atccaacgta acgggtaacc gtttatttcc
     5881 tattgcaatt ggcattagta ctctctcctt tcatcgaaat aaccctatga aaaatatggc
     5941 tgcaatcatc ttgaatagta gactcacaag ggctaataca aagcgccaaa agatcaaaca
     6001 aaataccagt attgccaaaa atactacgaa gtttaaatgc acagctgcag catggcgcat
     6061 aagtatttcg ccaacaaata ggactaacaa tagaggaatg attttattca tatggatctc
     6121 cttctttctc ttaggttatc gggtgttgcc cgcttatttc gcaccaccta agcggcgggg
     6181 gcggttggag cgattggtgc gaaataagaa ccattccctt agttactagg ttttttgtcc
     6241 ttatttcaca cattctcact tttcggtata aatgattatt gccttttttt cctttcaaat
     6301 cgttgatatg gcattcgttt acggtcaagt tcattttgga tttcttctgt atcgtcgtaa
     6361 gtataggtgt ctaaaaaatc agttaaagtc gtgtaccagt cgtcgcctgg gttatctact
     6421 tcttctacat attccctcaa aaatgcttcg ggtacagcga gttcataatc cattcccatt
     6481 aacacgatag attccgttcc acacgtcaaa aatctagtaa atacaaggta ataatcgtct
     6541 atcgtcgcat ctttgctgtt tagctcttcc atttcttctt ccattaattc catgctctca
     6601 aaaattgaat aatattctga ttctgagtaa tagtgtttcc tgcaaatcgt gtagctgaca
     6661 aagttttcga ccagctcact cctttctagg taaacattca tgatatattt ttctgtttct
     6721 agtcgtagaa gctccgttcc tttttttacc gtttcaggac gacgtttata cacgtctaaa
     6781 cgattatggc tgactgtgac cttcgtttga ctcgtttcac tttccaaata caattgttct
//
LOCUS       DACJGB010000112         6667 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.116, whole genome shotgun sequence.
ACCESSION   DACJGB010000112 DACJGB010000000
VERSION     DACJGB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.116"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            275..871
                     /locus_tag="IYI34_002775"
     CDS             275..871
                     /locus_tag="IYI34_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAR0560597.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(929..1411)
                     /locus_tag="IYI34_002776"
     CDS             complement(929..1411)
                     /locus_tag="IYI34_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560598.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1517..1714)
                     /locus_tag="IYI34_002777"
     CDS             complement(1517..1714)
                     /locus_tag="IYI34_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560599.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2009..2767
                     /locus_tag="IYI34_002778"
     CDS             2009..2767
                     /locus_tag="IYI34_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAR0560600.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2827..3255
                     /locus_tag="IYI34_002779"
     CDS             2827..3255
                     /locus_tag="IYI34_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560601.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3301..3373
                     /locus_tag="IYI34_002780"
     tRNA            3301..3373
                     /locus_tag="IYI34_002780"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3334..3336,aa:Gln,seq:ctg)
     gene            complement(3523..3894)
                     /locus_tag="IYI34_002781"
     CDS             complement(3523..3894)
                     /locus_tag="IYI34_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAR0560602.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4109..4294
                     /locus_tag="IYI34_002782"
     CDS             4109..4294
                     /locus_tag="IYI34_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560603.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4377..4565)
                     /locus_tag="IYI34_002783"
     CDS             complement(4377..4565)
                     /locus_tag="IYI34_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560604.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4589..4852)
                     /locus_tag="IYI34_002784"
     CDS             complement(4589..4852)
                     /locus_tag="IYI34_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560605.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5010..5267
                     /locus_tag="IYI34_002785"
     CDS             5010..5267
                     /locus_tag="IYI34_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAR0560606.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5338..5529)
                     /locus_tag="IYI34_002786"
     CDS             complement(5338..5529)
                     /locus_tag="IYI34_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560607.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5670..5990)
                     /locus_tag="IYI34_002787"
     CDS             complement(5670..5990)
                     /locus_tag="IYI34_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560608.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6013..6447)
                     /locus_tag="IYI34_002788"
     CDS             complement(6013..6447)
                     /locus_tag="IYI34_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAR0560609.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2088 a         1300 c         1019 g         2260 t
ORIGIN      
        1 atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta tatatcagct
       61 gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata actaagaaaa
      121 tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta ttttttgtga
      181 gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat caattaaaaa
      241 aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg acactttgaa
      301 aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg aagaattaac
      361 aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc aatcaacacg
      421 cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg aagaagcact
      481 tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag ctggtttcaa
      541 aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag gtctacaaga
      601 acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa acggaatcgc
      661 aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact tgcaacatta
      721 caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa actggaaact
      781 acgttcgcaa ttagtattcg gttcaccaga aacaccggct gaggaaaaag aatacatgaa
      841 cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa cagaggttgt
      901 gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata tcaatctctg
      961 cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat gcagaaaatt
     1021 tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt ttagaacctt
     1081 ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt ttgtcaaagc
     1141 tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa ctaataaaaa
     1201 tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg atgagtgtat
     1261 accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga tttcgcacag
     1321 tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca acaacgaata
     1381 aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt tcttctccag
     1441 aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca gcacgccgca
     1501 cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg cttgcttttt
     1561 catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg gcgcagtttg
     1621 tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt ttgcatgcat
     1681 attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac tgctggaact
     1741 tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt ttatttacag
     1801 ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa ttaaaacagc
     1861 tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa aactaattat
     1921 ttcctacata ttaaaatata atacttccat ttccaataaa aatacattta taataggaaa
     1981 gataaacagc tgaaaaggga gatttcaaat gaagaaaaaa acgttcaaaa aactattgtt
     2041 tctagggggc acagctgctc ttttattaag cggtacaagt tattacctac atatccattc
     2101 tccagaacat acaatgaaca ctgcacaaaa aagttcaaca caaaaaacga aaaagacaaa
     2161 agagaaagca gaagttttac tagatgttcc attagagagc cagtttgatt ctccttcttt
     2221 agaaaatggc tgtgaagtca cttcgctttc tatgctttta tctttttatg gttatgaaac
     2281 aactaaaaac cagttagctg agcaattgga ttacgtccct gttttcaatg cagatgggac
     2341 tcatggcgat cctaatgaag gatttgttgg tgatatcagt ggcggagact gggctatggt
     2401 cgtctatgtt cccccagttg cctcccttgc tcagcaaatc gtccaaacgg attatcacac
     2461 ttttccaaaa accgatgctc aactcgatga tatcaaaaaa gcactatcta aaggaaatcc
     2521 tgtctggact tctgtgacta tcaattttga agtcccagat gaaactgatt ttatgacttg
     2581 gaccacaaat aatggagaag taagagtcac tcctcttgtt catgcttgtg tagtcactgg
     2641 atatgacaaa gaaaatattt atgtcaacga tccctatggt gtaaaaaatc gagcagtacc
     2701 gatcaacgat ttttcggcga ttttcactgc aatgggtgga cagatgctaa ctttggagaa
     2761 aagctagtaa ttcaaacaaa aatcccttat actcaagata ccaaaccttt gaagggagcg
     2821 tagataatgg aaaaattcac acataaaaaa atggatccaa atgaaattcc gattattttt
     2881 gtccgtgact gcaagggaaa tgttcaaggt aaagtctcta ttaatgagtg gaacgagcgt
     2941 cgcagacctg ctaccctcaa tgaactggaa atcaaattgt atcgtcaatc tcttgtttat
     3001 tacgcagatc aagagtacga gaaagccacc gatttattga aatttctaat tgctcgaaca
     3061 gagtatactc gctttgaata tattgaacgt ttagcaaaca tctatcatat tatgaatgaa
     3121 cctgtcaaag aatatcagtt attggacacc gtcttgtcag tagcagaact gattgccctt
     3181 cctgctggat tagaaaagaa actagtacgc cgccttttac gagtaaagca acaactatcc
     3241 gatcaagaaa aataacttgc gtttttttag gaaagtgcta tgattatttt gttgaataaa
     3301 tgggatgtag ccaaactggt aaggcacttg actctgaatc aagcaattgt aggttcgaac
     3361 cctgccatcc caatttgatg tgcttgctga aaaaatgaac cccctgtaaa agaacaatct
     3421 ttttgcaggg ggattattgt attattttta ctacttgtat ttagtttaca taattaaatt
     3481 atgaataata aaatcgggcg tattcttaat acaaaaactg atttacccaa taattgctaa
     3541 tgctttctcc ggaacttttt ctctaggaac ttcctcatac gtttctatgt gtgctccttt
     3601 attgctaatt ttcaaataat gattttcttt taacacagaa agaccattaa attcaaccgt
     3661 tcgttctttc ccattggcat cagcagcttt ctgcacatac cttgctgtac catattcatt
     3721 tacttcctct ggtttttgtg tctttacata gacctctcct ttttcaacta atggatttag
     3781 ctgatcaagt ataccaggga tttctccata acttccttca gtataatatt tcagtccaaa
     3841 tagcaaaata ccactaatcg ccgccagtcc aatcaaatat ttaatcactt tcatctttct
     3901 acctccttgt tatatattca gtatatgggt cagttaaaga aattccatcc gtcccaagtt
     3961 ggagattttt ctcctacttt agaagcgaga atcgagtgtt tttttagtaa aaatgatcgt
     4021 tatttgatat tattgacgtg tggataaatc agtttttctc gttttgtcca tgtaaaaaat
     4081 catgttgata gcaaaggatg tgttcaaaat gaatcaagaa gaattatttc aaaaagtcaa
     4141 ggaaatgatt aagaacggca atttcgatgg cgcaaaacga tttatcgaag aacataaaga
     4201 acaattagga ccttataaag agaaagccca aaatctttta aaagatgtaa atatcgacag
     4261 tgtcaaaaac aaatttaaaa atctattcaa ataagtaaaa ctttcacttt tgtaaaatca
     4321 aaggaggaca atgctgctgc atcgtcctcc tttggttctt ttctgaatac ttcatactaa
     4381 aataaacgtg tcggatcgta atgataagtc acattgccgt tcagtgaggc ttttcccacc
     4441 acatatttac gattctccaa cttcagccac gcttctcgaa aagccattaa ttcttctctt
     4501 tttacatcaa tcttttcgat ttttccatcg atcaaatctt gaataagttg ttcatatgga
     4561 ttcatttttt tcctccatct gctgtcaatc aataagttgt aaaaataggt tcatatgctt
     4621 ttttggtatc tagatcttct ctgactttga ctgtcgtata aataattttt tctactggga
     4681 tactataata acttccttca ccattattga atcggtgaac gtctttcttt gctaaaatac
     4741 catttatttt attcgtcagt acacgttgtt cgacactgtt acttacaaca tagtaaatca
     4801 cccgaatatt attgatcaaa tgcaaatcga tttctaacac tacatgatcc atgaaaatcc
     4861 cactccttct ttcactttga ttatagcaag tttctcctct aatttcaaaa tcgatacaga
     4921 ttttaccgga taaatccaca aaactgcttg cgccatttcg cacaaagtgt tattttgtat
     4981 catggacttt atttatcgga ggcaatagta tgatcgcgat cggtcaattt gttttttata
     5041 ttcctttttt tattatgata agcattcttt tctattacat aaaatggaca aaaaagaagt
     5101 tttcagtttt actggcctct ttacctgctg tatattttac ttatcaaatt ttttctttta
     5161 ggcattggga aacaacgtct gtattaataa cacacatcat cgaattgacg ctttcagtaa
     5221 tttttcttat tatttggatc tattttttat ataagaatca gaattgaatc cctcgcatgt
     5281 ttaaaaagaa aacgattaaa gatgcagcaa atttctgccg atctttaatc gttttttcta
     5341 ttctgttata ccaaaaaaat gtttgaatcg gtgaaggatc gttccttgtt ttaattctat
     5401 aaattcttgt gcgatcgggt acttttcttc caaattttct ttgttctttt cgatcaggct
     5461 tttcgcttct tcatattttt tatctgcaca tagtgctttt acttcctgta atacttttcc
     5521 tgcttccatt tgcctcaccc tcttgccatt ttattgcttt aagttttatt taagttatac
     5581 gcattattct agtttcatcc caaataacct tttttcattt ttactcctcg aactaatcag
     5641 ttttctaaag gctgattggt tcgtcttttt tatttttcct ccatttcatt ctgctcttct
     5701 gttaatttat ctaatgcttt gattgatgct gcggctcttg cataatcata gctgtataat
     5761 tccataaatt tatcaggcag aacaaaccac gctggatgat aaccgttagc tgtgacaggg
     5821 aatcctttat cttgttggtt atctccatcc catctcagag caacaccttt ttttcctttc
     5881 cactttacta aagcgatcga gtatcccttc tttgtttgtg tgccgtcttc aataattgct
     5941 aaaatttcta ttttttcttt tggcgcacgg acttcttgag gacgaaccat tttctattcc
     6001 tcccttatct cattacttca cgaccaacac actgcaaggg gcatgtgata cgacataaga
     6061 agcggtcgat ccaactaatg cttgttgtat agcacctttt cctgtcgctc ccataacgat
     6121 caaatcaata ttttcttgtc cagggatgac attggcaatc aaatttcttg gatctccagt
     6181 ttcaactatt gcgtggatct cagcaatgcc gtactctttg gcatcattga tttttttcag
     6241 catggctact tctgttcttt gtttttcatc tgcatagatt tttgaaaagg cataggcact
     6301 atgggtcagc tcaactttgt tgatgattga cacgataaat aagtgggctt cattgtcctt
     6361 tgctaagtca agggcttcag cgaacgcttt gatcgattgt cttgatccgt caactgctac
     6421 catgatgttc tggtatcttt tactcattgg aaccgcctcc tttttctttt attgtaacat
     6481 ctcaagcaaa aatacaaaaa atatacgcaa tatgtaaata aaatgatgaa caaaagcaat
     6541 ttctttcaag ttatttatac atttcttttc tcatcagaac acctgttcta atcccattat
     6601 ttctgcatta tgtgaaattc ctcatcattt ttcacatcga gcgaacatca ttctgttaca
     6661 aaaaacg
//
LOCUS       DACJGB010000113         6643 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.117, whole genome shotgun sequence.
ACCESSION   DACJGB010000113 DACJGB010000000
VERSION     DACJGB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.117"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(1..859)
                     /locus_tag="IYI34_002789"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IYI34_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IYI34_002790"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IYI34_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAR0560610.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IYI34_002791"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IYI34_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IYI34_002792"
     CDS             complement(2143..2373)
                     /locus_tag="IYI34_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAR0560611.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IYI34_002793"
     CDS             complement(2603..3421)
                     /locus_tag="IYI34_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAR0560612.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IYI34_002794"
     CDS             3582..4271
                     /locus_tag="IYI34_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAR0560613.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IYI34_002795"
     CDS             4285..5787
                     /locus_tag="IYI34_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560614.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IYI34_002796"
     CDS             5800..6276
                     /locus_tag="IYI34_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAR0560615.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IYI34_002797"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IYI34_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACJGB010000114         6387 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.119, whole genome shotgun sequence.
ACCESSION   DACJGB010000114 DACJGB010000000
VERSION     DACJGB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.119"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(560..1600)
                     /locus_tag="IYI34_002798"
     CDS             complement(560..1600)
                     /locus_tag="IYI34_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAR0560616.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="IYI34_002799"
     CDS             complement(1949..2275)
                     /locus_tag="IYI34_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560617.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3050)
                     /locus_tag="IYI34_002800"
     CDS             complement(2262..3050)
                     /locus_tag="IYI34_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR0560618.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3482..>6387)
                     /locus_tag="IYI34_002801"
     CDS             complement(3482..>6387)
                     /locus_tag="IYI34_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAR0560619.1"
                     /translation="LHEKDLELMETIRRPATRLGFALQLVSLRFLGTFILDLTNIPQN
                     VLSYVATQLNIDPGEIIYYSRRQTRYQHTKLIKSHYGYQDFNQPEIETYLANWLLNRA
                     LYTTESSDMLFDMLLKKCLDEKIVLPGITTFSRFVASAIEKAEEHLYRQLSLVPTEDE
                     RKRLLNHLELIGTPVYGAIIKMDILRTPLADDSHKEIARGFDRLKQFQAFSTEKWKME
                     SIPEGKIKVLANYAFKAKAQLIQRMSEQKKLALLVAFIFIYKRKAMDEQILALSNFFE
                     TVFRRAKNKESKERLRTIKDLDRAASTLSDIVDLVMSSHSDTNELSLRDRILEKYSEE
                     NVTEAVSQVRLLVKNDREPIAISELLNTYRKFRKFIPDILTTLTFDSNDYGNNCKELW
                     TFINNRFPKPITYKMFGAIKHTLPKKWIYYIHEYPEQTNKCVLIAAVELLIQSLKRHD
                     IYIPQSVLYNNPMDCLIDEKTWVQQKYVLLEQLDLPSSANEMSLWLKNDLELSYKEAL
                     NRWPTSKMARIENADRLVVSRLKKTYEKKEDKSFRRRIQQLLPKIDLSDLLLEVNQQL
                     NLTQSFNHLSDKDTKMKDLDISLLAVLLSESCNIGFSPVSKEGFDSLKYDRLVYVNHH
                     YIRVDTLSGANQKIIHAHRKLATSKIWGDGHMASADGIRYITPQRSLYSRSNPKYFGR
                     GRGITFYNFVSDQYIGFHGMVVSGTLRDSLYLLEGFLNQPSELTPNQIMTDTAGYSDM
                     IFGLFGLLGFQFSPRIANDKGTKLWRIDANAHYGELDTLSQNSINMELIYTHWEDILR
                     VAGSLKSGKVNATELTKSLQRNGQPTPLGKAITEYGKVYKTKHQLRYISDEIYARQIL
                     EQLNKGEARHALCRNIFYGRKGKLYQTYYDGMEEQLNALGLVTNAIIYWNSLYLEKVF
                     EQMKAEGFDCSEEMIRRQSPLISDHINFVGKYTFRYEAELSNGKLRPLNVEEF"
BASE COUNT         1948 a         1119 c          995 g         2325 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgtggg gtacatggaa catagaaacg aaatagtgcg gtagcacaaa
     3361 agtaaaaaca caagaaacgt tgatttaacg ctgttctcgt gtttttctct ttttctattc
     3421 gaagtgattc gctggaacag tcatacgaaa tagtgcggta gcatgcaaaa cccgaacatt
     3481 gctagaattc ttctacattc aatggacgta acttaccgtt ggataattca gcttcgtatc
     3541 taaacgtata cttaccaaca aaattgatgt gatcactaat tagtggagat tgcctgcgta
     3601 tcatttcttc ggaacagtcg aatccttcag ctttcatttg ttcaaatact ttttctaaat
     3661 acagtgagtt ccaatatatg atggcattag taactaatcc taatgcattg agctgctctt
     3721 ccatcccatc ataataagtc tggtagagtt ttccttttcg tccataaaaa atattacggc
     3781 ataaggcatg ccgagcttcc cccttattca attgttctaa aatttgccgt gcatatattt
     3841 cgtcagaaat ataacgaagc tgatgtttcg tcttataaac ttttccatac tcagttattg
     3901 cttttcctaa aggcgtgggt tgcccattcc gttgaagaga tttcgttaat tctgttgcat
     3961 tcacttttcc agatttcaac gaacctgcaa ccctaagaat gtcctcccaa tgggtataaa
     4021 tcagttccat attaattgaa ttttgagaca acgtatcaag ttccccgtag tgagcgttag
     4081 cgtcgattcg ccaaagtttg gttcctttat cattggcaat tcttggacta aattgaaacc
     4141 ctaataagcc aaagagacca aaaatcatat cgctataacc agctgtatca gtcatgatct
     4201 gattaggagt taattcgctg ggttgattga ggaatccttc tagaaggtaa agcgaatctc
     4261 gcagtgtccc tgaaacgacc attccgtgga accctatata ttgatcagag acaaaattgt
     4321 agaatgtgat cccacgacca cggccaaaat attttggatt acttctagaa tatagggaac
     4381 gttgtggtgt gatataacga ataccgtcag ctgaagccat atgcccatca ccccatatct
     4441 tagaggtggc aagtttcctg tgtgcatgta ttattttttg attagctcct gaaagtgtat
     4501 ccactcgaat ataatgatga ttgacataga caagacgatc atactttaaa gaatcaaatc
     4561 cttcttttga aaccggggaa aaaccaatat tgcaggactc tgataaaaga actgctaata
     4621 aactaatatc caagtctttc attttggtgt ccttgtcact taagtggtta aatgattgag
     4681 taagatttaa ctgttggttt acttccaata acaaatctga caaatcaatt ttaggaagta
     4741 actgctgaat gcgacgtcta aaagacttat cttccttttt ttcatatgtc tttttaagtc
     4801 tagagacaac caggcgatct gcattttcta ttctggccat tttagaagta ggccaacgat
     4861 ttagcgcttc tttataagac agttccaaat cgttctttaa ccataaagac atttcatttg
     4921 ctgaagaagg aagatctagt tgttctaata aaacgtattt ttgttgaacc caagttttct
     4981 catcaatcaa gcaatccata ggattgttat ataagacact ctgaggaata taaatatcat
     5041 ggcgttttaa agattgaatt aacaattcga cagctgcaat caatacacat ttattagttt
     5101 gttcagggta ttcatgaatg taataaatcc atttctttgg aagagtatgt ttaatagctc
     5161 caaacatttt gtaagtaatc ggcttaggaa atctattatt aatgaaggtc cagagttcct
     5221 tacagttgtt accgtagtca ttgctgtcaa atgttagtgt agttaaaata tccggaataa
     5281 attttctgaa ctttcgataa gtgttcagaa gttcagaaat ggctattggt tcacgatcat
     5341 ttttaaccaa tagccgaact tgcgatacag cttcggtaac attttcctca gaatattttt
     5401 ctaagatacg atcgcgaagg gatagttcat tagtatcaga atgactggac attactaaat
     5461 ctacaatatc tgaaagtgtc gatgcggctc gatctagatc ttttatggta cgaagacgtt
     5521 ctttagattc cttatttttg gcgcgtctaa aaacagtttc aaaaaaatta gatagtgcta
     5581 aaatttgttc atccatagcc ttacgtttgt atatgaaaat aaatgccact aacaacgcca
     5641 gttttttctg ttcactcatt cgttgaatta attgagcttt agctttaaat gcataatttg
     5701 caagaacttt tattttccct tcaggaatag attccatctt ccatttttca gtagagaaag
     5761 cttgaaattg ctttagtcgg tcaaaacctc tggctatttc tttatgacta tcgtcagcta
     5821 acggggttct caaaatgtcc attttgatta ttgccccata aactggagtt ccaattaatt
     5881 ctaaatgatt gagaagccgc tttctctcat cttcagttgg aaccaaagac aattgtcggt
     5941 ataaatgctc ttcagctttt tcaattgcac tagcaacaaa acgagaaaaa gtagtaattc
     6001 caggtaaaac aatcttttca tccagacatt tttttagaag catatcaaac aacatatctg
     6061 aagattctgt tgtatacagc gctctattta aaagccagtt agctaggtat gtttctattt
     6121 ccggttggtt gaaatcttga tatccataat gtgattttat caattttgtg tgctgatacc
     6181 tggtctgtct cctgctatag tagataattt ctccaggatc aatatttagt tgagttgcta
     6241 cataactcaa tacattttgg ggaatattgg taagatctaa tataaaagtt cctaaaaaac
     6301 gtagtgagac taactgtagt gcaaaaccta atctagtagc tggtcgtcgt atagtctcca
     6361 tcaattctaa atctttttca tgcaaat
//
LOCUS       DACJGB010000115         6377 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.120, whole genome shotgun sequence.
ACCESSION   DACJGB010000115 DACJGB010000000
VERSION     DACJGB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.120"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            320..1165
                     /locus_tag="IYI34_002802"
     CDS             320..1165
                     /locus_tag="IYI34_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0560620.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1446..2666
                     /locus_tag="IYI34_002803"
     CDS             1446..2666
                     /locus_tag="IYI34_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAR0560621.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2663..5383
                     /locus_tag="IYI34_002804"
     CDS             2663..5383
                     /locus_tag="IYI34_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0560622.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5367..6311
                     /locus_tag="IYI34_002805"
     CDS             5367..6311
                     /locus_tag="IYI34_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAR0560623.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2372 a          986 c         1229 g         1790 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt
      601 cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta
      661 taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta tcaaagagct
      721 aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga
      781 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
      841 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      901 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      961 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
     1021 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
     1081 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
     1141 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
     1201 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
     1261 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
     1321 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
     1381 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
     1441 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
     1501 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
     1561 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
     1621 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
     1681 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
     1741 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1801 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1861 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1921 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1981 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     2041 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     2101 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     2161 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     2221 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     2281 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     2341 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     2401 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     2461 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     2521 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     2581 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     2641 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     2701 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     2761 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2821 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2881 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2941 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     3001 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     3061 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     3121 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     3181 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     3241 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     3301 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     3361 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     3421 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     3481 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     3541 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     3601 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     3661 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     3721 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3781 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3841 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3901 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3961 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     4021 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     4081 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     4141 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     4201 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     4261 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     4321 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     4381 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     4441 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     4501 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     4561 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     4621 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     4681 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     4741 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4801 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4861 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4921 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4981 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     5041 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     5101 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     5161 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     5221 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     5281 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     5341 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     5401 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     5461 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     5521 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     5581 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     5641 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     5701 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     5761 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5821 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5881 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5941 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     6001 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     6061 tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt ttagcacacc
     6121 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     6181 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     6241 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     6301 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     6361 tcgctttttt tgttttc
//
LOCUS       DACJGB010000116         6230 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.121, whole genome shotgun sequence.
ACCESSION   DACJGB010000116 DACJGB010000000
VERSION     DACJGB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.121"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            133..633
                     /locus_tag="IYI34_002806"
     CDS             133..633
                     /locus_tag="IYI34_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAR0560624.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            730..2577
                     /locus_tag="IYI34_002807"
     CDS             730..2577
                     /locus_tag="IYI34_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAR0560625.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2580..3476
                     /locus_tag="IYI34_002808"
     CDS             2580..3476
                     /locus_tag="IYI34_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0560626.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3526..3915
                     /locus_tag="IYI34_002809"
     CDS             3526..3915
                     /locus_tag="IYI34_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAR0560627.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3902..>6230
                     /locus_tag="IYI34_002810"
     CDS             3902..>6230
                     /locus_tag="IYI34_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAR0560628.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2106 a          925 c         1336 g         1863 t
ORIGIN      
        1 gagttttcca aactgattgg cttcttggtg attgctttga ttgcggtagg tctggtcttt
       61 aatgcttctg gtgtgaagga tgtcttactc aatttgttta accgaattgt cggtgcctga
      121 gggggaatca caatggaaca gatacgtgtt tatattgcga accttggaaa atacaatgaa
      181 ggtgagttgg tgggggcatg gtttacccca cccgtcgatt ttgatgaagt gaaagaacgg
      241 attggattaa atgacgaata tgaagagtac gcaattcatg attatgaact gccttttgag
      301 attgatgagt atacaccaat tgaggaaatt aatcggttgt gtggtttagc ggaagagctt
      361 aaaggaacgc caattggtga ggtcgcttca gagattcaac atgccttctt caattctttc
      421 gaggaaatgg tagagcatgt ggatgatatc atctattatc ccgattgtaa tgacatgagt
      481 gatgtagcgt attacttgat tgtagaagct ggtgatttag gcgaagtacc agaacacttg
      541 aagacgtata ttgattttga tgcgtgtggt cgagacttgg agattggcgg gaattatttg
      601 gttacgagtc gaggggtttt tgaactaatg taaatcattt gttagaattg accagaaatt
      661 tctatcgtac ttttactttt ctatttgata tactgaaata gaaaatattt gttgataggg
      721 gtgttactaa tggttggcaa taagtggtac aaatgtgatc ttcatattca ttcaactgcg
      781 agtcagtgct ttcaggataa aaaggttact gctaaggaat gggtagaaga atgtttaaac
      841 aaaggactcg attgtgttgc attaactgat cacaatacag gggaaaatat tgatgaatat
      901 aagaaagtgg ctatcgaaaa tggcttagat ttttttcctg gagtggaatt aacgtgtgga
      961 gaaaatggaa ctcatttact agtcctattt gataaaagtg catgtacaaa tgatatctct
     1021 gatttcttaa ccaagatggg tataaaaaga gaggattggg ccagcgctgc agcttgctca
     1081 atgaaatcag ttttagaagt tattgatttt gctgagcacg agggcttgct cgttattcct
     1141 gcacatatag atgagtttaa tggaatttct tcagcaaaat ttggaaatct aggaaagata
     1201 tttgaatcag aaaatatata tgcagtacag acggttcaaa aagaattttt tgaaaatcgc
     1261 tcacaaatga ttccctcatc taagcgtgac actatctatg atagtgtgaa tgccagatat
     1321 gatggtagag ttggaaaaga tactttggaa tcttggtata aatcagttgt tgaggcagaa
     1381 aaaaataata tgacatttct tagcttttct gataacccac actcgaaagg aaattcaaaa
     1441 catggacttt ggggaatagg cacaagatat tcctatataa aaatgaagga cgaacctgac
     1501 ttgggatcac ttcgcgatgc tttaatgcta ggcgaaactc gagttcattc tgatttttct
     1561 aatttctcca ttaatgaaaa tgaagtttta ttggaaaaat taagcttttc tgggaccact
     1621 cttagcacaa aagaagttgt agtcgaattc agcgataatt taacttcaat tattggagga
     1681 agaggaacag gcaaatcttg tattacaaga ttcttggtct atgtccttgg aaaggaagca
     1741 gaacttgatc aattttctga aatacaaagt gattatcaga actttgcaca aattgaacat
     1801 aatggctctg gaatattttt gaaggataca attgtgaaac tgaatatttt ctataagggg
     1861 acaaaatatc aaataataag aacacaggat agacattcta tttatgaatt tacacaagag
     1921 aatggtctgg ttgaaaaaga aacagaacgg ttgagaatga tttccgataa ggtgagtata
     1981 tactctcaaa aacaaattta tgaaatttca aaaaatcaaa ctagtatact agaattacta
     2041 gatggatata actctgattt gatttcagag tataaaaatg agattgaaac atgtgtaaac
     2101 gaaataagaa aattaaattg ggatattgtt tctgtgaaaa aagaaataca agataaggcg
     2161 aaggtagagc ttgaaattga agatttaaaa aaacaagtag aaaagctatc tcataagagt
     2221 tataaagatg tttatgatga gtattcaaaa gagacagata tttataggga actgaaacgt
     2281 gatgtggagg cacttaagga aattcccaaa aatctaactg atagtgttga caaaattgac
     2341 tttggcaatg gtataaaagt tacagacgaa attgatgaac accgcggaga attgattaag
     2401 aatttagtga ccaatcgggc taagattcaa acaataatta atgaaatgtc agatgaaatt
     2461 gatagttata gagcagagct gaataaatca gattggaaag ttaactataa tgaggtttca
     2521 aagaggtatc aaagaggtat caaaaactta gtgaagagtt atctcaagat gaattaaaaa
     2581 tgttacaaaa ccttgattct ttatatcaat ctttatcaaa aactgaagag cgattggaag
     2641 aaattattca acaagaaata aagcttaaag attaccaagt tgaggttgac tcagcaatgt
     2701 caagaatgaa taaatcatat caaaatctct attctgagag aagtagttcc tcgcgcgaaa
     2761 ttttttctga tattacagga atagaggcta ctattcaacc aaatagggat ttcaataatt
     2821 atgttaaaga gctcagagct tgtattaata aggaggacac ttttgacagt gagtttggat
     2881 ctcttattga taagctgaca aaaaattcca ttactaatca agatatatat aatgacatac
     2941 ttgatataag agttagtggg gaaagtagta ttttttcaga taaaaaatta atcaattcta
     3001 taagaaaatt gaatgatgca caagttcttg aattacaaac ccttaagcca aatgatttag
     3061 tagttatccg acttgaacta aatggaaaaa aagttagttt atcgaatgct agtgctggtc
     3121 aaaagacatc tgcaattctt tctatgattt tggcgtatgg aaattcacca ctcatattgg
     3181 atcagcctga agatgatttg gatagtcaat taattaataa cttaatcgta agaagtatca
     3241 ttacaaaaaa ggaaaacaga caaattttaa ttgtaactca taatgcaaat atcccagtta
     3301 atggtgattc agaatggttg attagcatgg gagattcaaa agaaatatcg gctgatcagt
     3361 gcgggtcggt agatgaaccc aatatcaagc caagaatatg tactgtgatg gaaggtggcg
     3421 aggatgcttt tacaaataga gcgaaacgct atggttttaa acaaattgta gaataacact
     3481 agagggtgag acatattatt atgtccgccc tttttaggag gaaaaatgaa aaaaataaaa
     3541 agctatacca gtatctgggc agttgaaaaa gtcatttacg caatcaatga ttttcagttg
     3601 ccttttcctg tgacttttaa ccaaatggct tggttcgttt tatctttact gtttgtcatc
     3661 gtatttgccc atgtgcctcc actatctatg atagaggagg tctttttaaa atacttaggc
     3721 ataccggtgg ctgtcacttg gtttatgtcg caaaagacgt ttgatggcaa gaaaccactg
     3781 ggatttttac gatcctttat cagctatcac ttgcgattta aggtgacttt tgcaggaaag
     3841 aaagtcaaag aacagaagaa acgatgggat gaacccagta ctcttgtaag gagtgtgaac
     3901 tatgtatccg attaagtata ttgaaaataa tctcgtcttt aaccaagaag gcgagtgttt
     3961 tgcctactat gaattagtgc cttacaatta ctcctttttg tcacccgaac agaaatttca
     4021 agtgcatgat aactttcgcc aactaattgc gcaaaatcgt gaaggcaaga tccatgcgtt
     4081 acaaattgcc acagaaagta gtattcgcgc cacacaagaa cgatcaaaaa aagaaattac
     4141 cggtagactc aaagaagtag cgaaacaacg aattgatata cagacagatg cattagtatc
     4201 catgattggc gatagtcaga ttgattatcg cttttttatt ggctttaaac taattgcgac
     4261 agatgaagaa gtcaatctaa agagtctcaa gaaatctttc ttttctgggc ttcaagaatt
     4321 tgtctatgga gtgaatcatc atttgatggg cgattttgtt tccttatcca atgaagaaat
     4381 tcgctgctat tctaagcttg aaaaattaat ggaaagcaaa ttggctcgaa gatttaaagt
     4441 caggcgagtc acaccgagtg acctgacgta tttgattgaa catatttatg gcgaaaaagg
     4501 gataccgttt gaagaatacg aatttcagct accaaagaaa aagctaaaat cagaaacact
     4561 ggtgaaacgt tatgacttat tacgtccaaa tcgttgttta attgaagaaa aaccaaggtg
     4621 cttacggatg gaacatgaga atcatgagtc gtacgtagcg tatttaacaa ttaatacaat
     4681 tgtgggggag atggaatttc cttcctcaga acttttctat taccagcaac aacaatttac
     4741 attcccaatc gacacaagta tgaatgtaga gattgtgacg aataaaaagg ctttagctac
     4801 cgttcgtaat aagaaaaaag aactgaaaga tttagacaat catgcttacc aatctgacaa
     4861 tgaaacgaat tccaatgtct tagatgcttt ggattccgtg gatgaattgg aaaccacctt
     4921 ggatcagtca aaagaatcta tgtacaaact gagttatgtg gttcgagtga gtgccgaatc
     4981 agtggatgaa ctaaagcgac gctgtgatga agtgctcgat ttttatgatg ataccaatgt
     5041 aaaactagtc cggccctttg gcgatatgat ggggctacat gaagaattct taccctcaag
     5101 taaacgatat atgaacgact atattcagta tgttacgtct gattttttgg cggggcttgg
     5161 ttttggggct actcagatgc taggagaatt agaagggatt tactttggct acaatgtgga
     5221 tacaggtcgt aatgtttacc tgaaaccggc attggcttca caaggagtca agggttcagt
     5281 aaccaatgcg ctggccgccg ctttcctggg ttctcttggt ggaggtaaat cctttagtaa
     5341 caatctctta gtctactatg ctgttttgtt cggtggtcaa gctgtgattg tcgatccaaa
     5401 aggggagcga ggagggtgga aagaaacctt gccagaaatt gcggatgaaa tcagtatcct
     5461 caacttgacg agtaagtcag aaaatcaagg tctgcttgat ccttatgtga ttatgaagaa
     5521 aaagaaagac tcggaaagtt tagcaattga tattctgact tttttaacag gaatttcaag
     5581 tcgagacggg gagaagtttc ctgtgttacg taaagccatt cgaacggtga ctcaaagtga
     5641 tcaacggggg ttattacaag ttattaacga attgcgaaaa gacgggtcac caatcgcgga
     5701 aaatatcgcc gatcatatcg agtcctttac cgattatgat tttgctcatt tgcttttctc
     5761 agacggctcg attacccaat cgattagttt agataaacag ctaaatatca ttcaagtggc
     5821 ggatttggtt ttaccggatg cagaaacgac ttttgaagaa tataccacca tggaattgct
     5881 tagtgtttcg atgctaatcg tcatttccac ttttgccttg gactttatcc atagtaaccg
     5941 agaaatcttc aaaattgtcg atttggatga ggcttggagt tttcttcaag tggctcaagg
     6001 aaaaacgtta tcgaataagc ttgttcgtgc tggtcgggcc atgaatgctg gggtctattt
     6061 cgtcactcaa aatagcgacg atttattaga tgaacgttta aaaaataata tcgggttgaa
     6121 gtttgctttt cgcagtaccg atattcatga aatcaagaaa accttggaat tctttggttt
     6181 ggatcaggag gatcaaagca atcaaaaacg cctgcgagat ttggaaaacg
//
LOCUS       DACJGB010000117         6220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.122, whole genome shotgun sequence.
ACCESSION   DACJGB010000117 DACJGB010000000
VERSION     DACJGB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.122"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(203..1165)
                     /locus_tag="IYI34_002811"
     CDS             complement(203..1165)
                     /locus_tag="IYI34_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0560629.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1167..2606)
                     /locus_tag="IYI34_002812"
     CDS             complement(1167..2606)
                     /locus_tag="IYI34_002812"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAR0560630.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2791..4737
                     /locus_tag="IYI34_002813"
     CDS             2791..4737
                     /locus_tag="IYI34_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAR0560631.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5001..5873
                     /locus_tag="IYI34_002814"
     CDS             5001..5873
                     /locus_tag="IYI34_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAR0560632.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1986 a         1059 c         1106 g         2069 t
ORIGIN      
        1 caatttcgta aaacaaccgt aacagttgtg taaaaatatc attttagagc atgaaaaaag
       61 gagtggattt ctccactcca aaaaagtcta acttttgggg gtcactacaa gttgacaact
      121 cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg aattttatag
      181 acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag attggtaaaa
      241 tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca acaataagac
      301 ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat ttctcaatga
      361 aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt ttaagttgat
      421 gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata ccatctggtt
      481 tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac agattattag
      541 gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca cgtaattctg
      601 tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa ccattttttt
      661 gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca atggaaacat
      721 taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca taatcagcaa
      781 ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca agcatttcaa
      841 tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt ttatatccat
      901 gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata ttactaatat
      961 taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt gctaaattat
     1021 ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt ttttctgata
     1081 aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca gctaattcag
     1141 cgacatcagt taattttgct accattttaa tattttaatt caaagtattt tccagtaggt
     1201 tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg actagtaaac
     1261 actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa aatgttagca
     1321 gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt ggcatattga
     1381 atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac aatcaagcta
     1441 agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt tggaggaaaa
     1501 gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg ttcagaacta
     1561 gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc ttttattgaa
     1621 agttctttta caagactcat tgctccctga taatcatact tatcagttgg ataatcgata
     1681 tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt gaatccttga
     1741 gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc cacaagtttt
     1801 actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat attcccataa
     1861 tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt tttgtctaca
     1921 aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa atcaaggttg
     1981 ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt gataaagcct
     2041 gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata attaaacatt
     2101 tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac attctcacct
     2161 ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc acgttcccaa
     2221 ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat ttcatatgca
     2281 gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc tgctcctgtt
     2341 acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa cccatgagca
     2401 gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa agaaaaacca
     2461 ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg ccaaggtgat
     2521 cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga ataagcacga
     2581 tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt ttacaactag
     2641 tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata aactatatga
     2701 caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag tgaaccgttt
     2761 tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc ttccgacgta
     2821 atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac acgtcttcgt
     2881 ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa tccagatgtg
     2941 aaaagcacat ttaaaattga tggacaatat caagtgatta tcggtgcagg tgatgttaat
     3001 ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac agatgattta
     3061 aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat taaattatta
     3121 tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt aatggcatta
     3181 caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga aatgtatcct
     3241 gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt catgttctta
     3301 ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt tcttggtgct
     3361 gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata ttggaatatt
     3421 tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt tttagcagct
     3481 gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc atctattgac
     3541 tttacattca cgccattatt atcagtaatg attactggat ttttgacttt cacagttatt
     3601 ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg gctatataac
     3661 actactggat ttattggaat gggtattttt ggaggaacat attcacttat agtcatgact
     3721 ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg gacaaatggt
     3781 atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc tcaaggtgct
     3841 gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct tgcttcctct
     3901 gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt taacttaaaa
     3961 taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc agtagcagga
     4021 ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt tctttcaatt
     4081 aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt agttgctttt
     4141 gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc agaagaaatt
     4201 gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat atctactgat
     4261 gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat ttctggtaaa
     4321 gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat gggaaaaggg
     4381 attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac tattcaactc
     4441 acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga agttctaatt
     4501 catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa agttactact
     4561 aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat gattgagaaa
     4621 gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta ttcagaagta
     4681 aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat caaataaata
     4741 tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag attaatcaaa
     4801 tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa aaattattag
     4861 aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac ttgtcatgtt
     4921 actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga tttcattcta
     4981 aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc acaaaatttg
     5041 tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt ccgacaacga
     5101 cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa gcagatttag
     5161 cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc gccacttatg
     5221 ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc ggtaccatga
     5281 aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct gcctatggtg
     5341 agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg atcggtacgg
     5401 gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt catccagaaa
     5461 tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc tgtccatttc
     5521 atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaccgc ttagggaaaa
     5581 aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc tatattgctc
     5641 aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt ggcggtggtg
     5701 tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa ttggtcaatg
     5761 ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta gaagacaacg
     5821 ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca taaacgaaaa
     5881 aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt agtcataacc
     5941 agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc ttgaatgaga
     6001 taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggccacttg tattgtgcaa
     6061 tagtttttta caaaaaaaag ggggtgcgga tatttttttg gaccttttta acaaataggt
     6121 tctgttgcaa agttttaaat ctactatcag ataaggtaga ataatagaaa aagatagcag
     6181 gaggaatgac gatgagtcat tttaaaggaa agcaatttca
//
LOCUS       DACJGB010000118         6131 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.123, whole genome shotgun sequence.
ACCESSION   DACJGB010000118 DACJGB010000000
VERSION     DACJGB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.123"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            509..2353
                     /locus_tag="IYI34_002815"
     CDS             509..2353
                     /locus_tag="IYI34_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560633.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSNTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2409..3875
                     /locus_tag="IYI34_002816"
     CDS             2409..3875
                     /locus_tag="IYI34_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR0560634.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3912..4754
                     /locus_tag="IYI34_002817"
     CDS             3912..4754
                     /locus_tag="IYI34_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0560635.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
     gene            complement(4971..>6131)
                     /locus_tag="IYI34_002818"
     CDS             complement(4971..>6131)
                     /locus_tag="IYI34_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR0560636.1"
                     /translation="PEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRF
                     KCKSCQRTFVADTSVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVM
                     KRFYRPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLE
                     RYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRV
                     KETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADR
                     LLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQ
                     IQNALHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         1979 a         1027 c         1282 g         1843 t
ORIGIN      
        1 cgctaatcta attcactgta ttatcatttt ttagtagtat agcacaatta tgaatgcctt
       61 ttctttatat caaataaagt ttatataaaa aacctcatcc tgcaagaaag aaaaaatgaa
      121 cccattcttt ttatactagg tcttaaaaag acagaacatc tgctagaaga tagaagagaa
      181 taagagttat atatgttgga agatattctt agaaaaaaga caaaactttt agaacacgaa
      241 aactatattg aacgaataaa atgaaaaaat gattaaaaaa tcattttatt atttacgttt
      301 acattctaat atgttaaatt gaatataact taaacaggca tgtgactggt aatgcaggca
      361 ggacctaaat aagttgaata acaatgtatg cgtttcaata cacgtttgcc ttgttgtcaa
      421 tttatttagt ttctgcctgt tttttgtgtt gaggatggcc acttcgactc aaaagttcat
      481 cactcaaaaa agaaaagagg gaaacaccat gaattacaaa caaacagcag aagaagtatt
      541 gcagtacatc ggtggggaag aaaacatatc ccatttggag cattgctcaa ctcgtttacg
      601 ttttacgtta aaagacaaca gcaaggtaaa tatgagccag ttagaaaaag tggatggcgt
      661 tatgggcgtt cgccaaaatg tgcaatgcca agtaatcatt ggaaacgatg ttgttgaagt
      721 gtatgatgaa gtgaaagcgt tattaggtga caatgtttca aatactgaag aagcaccaaa
      781 acaaaaacaa aaattaggtt cagttctatt agattttatt atttctattt tccaaccttt
      841 aattcctgcg attgcaggtg gaggagtatt gaagtccttg ttgctactag cttctatctt
      901 agggctgatt agtgaccaat ctcaaaccta tcagatttta aatttggtcg gcggggcacc
      961 gctttatttc ttgcctattc tggtggcaat gactacggct aataaattga aagtcaatca
     1021 attggttgca gtatctgctg tcggtgcatt gatccttcct gaaatgacag ctatgttggc
     1081 ggaaggtgct acattcttat cttttggatt ggataatata gcgtatgcat ctcaagtatt
     1141 cccagccatc ttaactgttt tattttatgc tcaacttgaa aaatggttta caaaagtttc
     1201 accaaaacca attcgtattt tctttgtacc tatgatgagc ttattgatta cagttcctat
     1261 tgctttgttg cttctaggac cattaggtta taatgtagga acagctttct cttctatcat
     1321 tgtgtctttg tacaatcaat ttggatgggt tgcaaccgga gtcttagcag ctgtactgcc
     1381 attaatggtt gtaacgggta tgcataaagc aatgatccct tacgctgttt catccatgag
     1441 tgaattgggt gcagaacttc tttatctccc ggcttcattg gcacacaata tttcagaagc
     1501 tggcgcatgc tttgctgtca gtatcaaaac aaaagacaaa aaattgaaat ctacggctgt
     1561 ttctgcgggt atatctgcat tattcggaat tactgaacca gctctttacg gggtaacgat
     1621 tctgcacaaa cgtgttttat atgccgttat gttttctagc ctgatcgggg gagcatttgc
     1681 aggaatcgtt gcacttcaag cctttgcttt agtgggtcct gggattgcaa gtatcacgat
     1741 gttcgctgac aaagctgacc caatgaatct ggtttgggca tttgcagtat cggggatttc
     1801 atttgtggtt gcttttgctg cagcgttggt tctttataaa gataaagaag ttgtcaatga
     1861 agttgaaact aagatggaaa cggccgttgt ttcagatgct gttctttcaa gtcccgtgaa
     1921 tggaaaagta atctcactat cagaagtaaa agacactgtt ttttctagtg gtatggtagg
     1981 tgatggagta gcaatcattc ctgaagaagg gatattagta gctcctgaag atggagaaat
     2041 cacaatggta tttgaaacga agcatgctat tggtttaaaa acggctaatg gtgcagaact
     2101 tctttttcat attggtattg atactgttca gttagaagga aaagggtttg aggcatttgt
     2161 tcgttctggt gaaaaagtag ccaaaggaca aaagctgatt gcatttgata ttgatttaat
     2221 caaagaagca ggattcgacc ccacagttat ctgtgtggta accaataagg ataattacac
     2281 tatccgttca tttaacctag aaaaattggt aaccaatgat agtgatctct tagctatcag
     2341 tccgatagct taaacacaag atagtgaaaa aaaatatatt gaatgaagga atagaggaga
     2401 atagaataat gacaaacaca acaagttttc caaaaggatt tttatggggc ggcgcgatag
     2461 cagcaaatca agtagaaggt gcttggaatg tagacggtaa aggtctttca gtagcggata
     2521 tggctactta taaaccaaat ttagacaaca aagattttgc tgctcatatg catgtcaccc
     2581 tgcagtctat aaaagaagct agtgaagaca aatctgataa atggtaccca aaaagaaggg
     2641 gcgttgattt ttatcatcac tataaagaag atttagcttt atttgccgaa atgggcttta
     2701 aaaccttgcg cctctctatt gcttggactc gcttgttccc tacaggagaa gagctggaac
     2761 cgaatgaaaa aggggttgca ttttatgaaa acgtctttaa agaaatgcaa cgtttaggaa
     2821 ttgagccgat tgtgacacta tctcattatg aaatgccatt agctttagca gttaaatata
     2881 atggctgggt ggagcgtcga gtaattgatg attttgttcg cttcgctaac gtttgttttg
     2941 atcgcttcgg caaatacgta aaatattggc tgacatttaa tgagatagac agcatccacc
     3001 gtcatccatt tacaacagct ggaattatcc aagagaaaag cgcagaaggt caagaagctc
     3061 aagatatcta tcaagcattg catcatcaat ttgttgcgag tgcgttagtg acaaaagatg
     3121 ctcatgaaaa aattccagga agccaagttg gttgtatgtt aacgaaattg atgacttatc
     3181 cattaacttg cgcaccagaa gacgttgagc tgaccttgaa aaagaactta gaaaataatt
     3241 tttatgcaga tgttcaagtt aagggagaat atcctgctct aatcgaaaag gatttggaaa
     3301 atcggggaat tcagattaaa atggaagatg aagatcgaga aattttaaag aaacataccg
     3361 tagattttgt ttctttcagc tattacatgt ccatggcaga atcagctgat ccaaacgcag
     3421 aacgcacacc gggaaatact gttttagggg ttaagaatcc tcatttgcct tcaacagatt
     3481 ggggctggca aatcgatcca aaaggtttga aaatttcgtt gatcgaactt tatgatcgtt
     3541 accaagtacc gttgatgatt gtggaaaatg gtatgggagc agtcgatcag gtagtagatg
     3601 gtaaaatcca tgatccgtac cgcatcgact actttaaagc acacttccaa caaatgaaag
     3661 aagcaatcga tgaaggtgta gatttgatag cttatactag ctggggacca attgatttag
     3721 ttagtgcagg aacttctcaa atgtctaaac gatatggatt tatttatgtg gatgcagacg
     3781 atgaaggaaa tgggacatat aaccgcttga aaaaggattc gttcaactgg tacaaagaag
     3841 tgatcgaaac aaacggtgca tccttattcg agtagtctcg atctttttaa gcagcgatag
     3901 ggggataatg agtgatttca ataaaaaaag tattgaattc aagtgtggta cttgttgaag
     3961 aaaatggcaa agaaatgatt gcattaggta aagggatagg ctttggacag aaagctgggg
     4021 atgaaatatc aggagataaa gtagataaaa tatttctgcc ttttgaagaa aagaaatctt
     4081 cacaactatt agagttagtt gaagaaatac ctattgaatt ttttgaaatc actcgtacta
     4141 tcgtttttga agcagaaaaa ggattagata aaaaactgaa tcctggagtc tatttaacct
     4201 taacagacca cctgcatttt gcagttgaac gcgcacaaaa aggggtaaat attgcaaacc
     4261 gtttgtactg ggaaataaaa atctactatc ccaaagaatt tcaaattggg aagtacgcac
     4321 tcaccctttt gaacgagcaa tatggaattg atcttcctaa agaggaagcc tcaaacattg
     4381 ctttccattt gatcaatgca caatcggaag atcctgatag cggcgctgga ttcaagtatg
     4441 caaaaatgat cggcgggatc gtcaatttgg tccggtattc gttgcagaaa gatatcgata
     4501 ctaattcgat ccattatgcc cgcttcatta cacatgtacg tttctttgta gaaagatatt
     4561 attctgatgc tttgttggat gatggtgagg aagaattcta caatcaaatg tggatcttat
     4621 acccgtctgc catggaaagc gcagctaaga tcaaagacta cattgaaaaa gtttatcata
     4681 aaacaattcc gaacgatgaa gttgtatacc taggtgtcca catcaatcgg ttgatgaaac
     4741 atgctaatgg gtgagcgctc agtaatacta ttgatgcttt taaaatcttt agagatggat
     4801 tatagtaaaa acaaggtgat ttccagctgt ttgttatcta taagagacat ttaaaaagga
     4861 ttaacgtaag ataataaaaa atctcggctc tttgtcaaat gatgttggta aagagaaatc
     4921 ttagaataat ttggacagaa cagaaagaag aaaacctctt tctgttctgt ttatttggtt
     4981 aaaaatgttt tgccatgacg aatcataatt cgtagtttga aattgtaaaa gttgcgaaag
     5041 ccgtacgcat tgcgttttaa tactttgatg tggttgttta agcattccaa tggtccgttt
     5101 gaatagctgt agtgaagcgc attttggatc tgtggcagaa actttttaaa cgttcgtaaa
     5161 gtggttgtat aggtttctgg cagaccgcta atgtctctag ttaaacaatc ctcaaatagt
     5221 gactgatctc ttttctttac cgcataaaga atgtcttggt ataactgata gccttggcgt
     5281 aagttgggac aagcagctaa cagtcgatcg gcaatctcac tttcagtcaa ataatcttta
     5341 aaactggcac gccaaacccg tttttcatag tttaactttg cttcttcttt ttgtaggagt
     5401 ttccaatagc gcttcaattt tttccctaac cctcgttgag agtgggaatt tcctttaagt
     5461 cgtaccgtgg tttctttcac tcgatgattc aagaaagttc tgccaatatg ttggacgatg
     5521 tggaaacgat cgatgatcaa ctgagcattc gggaaaagct ttttcaccaa cgaaacataa
     5581 ggcgcataca tatcaataac aatcaattga acggcttcac gagtcgctaa tgaaaaccga
     5641 gaaaaatagc gttctaaata aggcaagcgt cgattttcga caatatccaa taatgcatgg
     5701 ctttgtccgt ccatcatgat aaaactcatt gcactggcaa cacttcgaac cgatttgaat
     5761 tcatcaaagc ataagacctt tggcaaagtc tgtttgaaag gattcaatgg gcggtagaat
     5821 cgcttcatga cacgatagac cgaagaagtg gaaatatttt tctgacgagc gatttccgtc
     5881 atggaagtat tctcttttaa gcgagcaatg acagaccaac gaactttttg actaatgaaa
     5941 cagtgttttt cagcaacaga ggtatccgct acgaatgttc tttggcaaga cttacattta
     6001 aatcgttgtt tcttcagctc cagataagtg cgatactcag acacatcatt cagtaacaac
     6061 cgaacggttg tcgtccccca acgaatgatc ctagagggga cgacacaaag acaatgggaa
     6121 cactcttctg g
//
LOCUS       DACJGB010000119         6073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.124, whole genome shotgun sequence.
ACCESSION   DACJGB010000119 DACJGB010000000
VERSION     DACJGB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.124"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(1..382)
                     /locus_tag="IYI34_002819"
                     /pseudo
     CDS             complement(1..382)
                     /locus_tag="IYI34_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            403..1605
                     /locus_tag="IYI34_002820"
     CDS             403..1605
                     /locus_tag="IYI34_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HAR0560637.1"
                     /translation="MCTKLLQNTAHKNPYKTVDEATQLDLVIVVNQLLTGYDSKWVNT
                     LYLDKSLEYQNIIQAFSRTNRLNGPEKPFGTIRYYRKAHTMEQNIEEAIELYSGKQAK
                     GLFVEKLYNNLEKLNDIFEEIKSVFNISGIENFSHLPEVPGEIKQFVNLYNQLIHCYE
                     SAMIQGFTWAKSTYEIQDYEPITVVITEYEFNILAQRYSEIEREPRDGNDSIAYDIDY
                     QLPEGSRSRINYEYLNTNFKKYMNIKEERDSNLIQSLLNDLHRTFAQLPVELQPYAEM
                     ILNDVNNGELVIKEGWEFTDYLNEYQLSEEDDFIQDLVDSTNINEALLREMLDLRLTE
                     VNINEYSRFDKLKSSVNVGHFSGYIEKNLGKMVIPIKVNMLIDRLLRAFLLEDVFDVT
                     EYIKNYFN"
     gene            2338..3306
                     /locus_tag="IYI34_002821"
     CDS             2338..3306
                     /locus_tag="IYI34_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAR0560638.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADARNHDLDVRRYMGKVEY"
     gene            3317..4132
                     /locus_tag="IYI34_002822"
     CDS             3317..4132
                     /locus_tag="IYI34_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAR0560639.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKKIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            4200..4910
                     /locus_tag="IYI34_002823"
     CDS             4200..4910
                     /locus_tag="IYI34_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAR0560640.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            4946..>6073
                     /locus_tag="IYI34_002824"
     CDS             4946..>6073
                     /locus_tag="IYI34_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAR0560641.1"
                     /translation="MKQVLRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         2067 a          920 c         1237 g         1849 t
ORIGIN      
        1 gtgcctccca tgtcgtggaa tctttgagta agagtttcca atatcgcttg agccgacggt
       61 acttcttctg attttcagag tgtgaggttc gaaaacgatt catgatctgg acacgtaatt
      121 gattgaagct gcgattgatg tgttggacaa tatgaaagcg gtcagttacg atctccgcat
      181 ttggaaagac cgttttaagc aactgacaat agctggcatt catgtccatc actaggtact
      241 tgacctttaa acgggccttt ctaggaaagc gcaggaagta ttgagttagt ttgtaaagtc
      301 tgcgatcttc aagggtcgta aatagctgat tggttgtccc atcaacacaa ataaaactca
      361 tggaaccaga agtggcaaca ttgtgtactt ttctttgcaa tcttgtgtac aaaactattg
      421 caaaacacag cgcataagaa tccttacaaa acagtagatg aagcaaccca attagatctt
      481 gttatagttg ttaatcagct cttaacgggt tacgattcaa aatgggtcaa caccctatac
      541 ttagataagt cacttgaata tcagaacata attcaggctt tttcacgcac aaatcgtctt
      601 aatggtcctg aaaaaccgtt cggaaccatt cgttattatc gtaaagcaca tacgatggaa
      661 cagaacattg aagaggctat tgagttatac tcaggcaaac aagcaaaagg gttatttgtt
      721 gaaaaattat ataataactt agaaaagttg aacgatatat tcgaagaaat taagtccgta
      781 tttaatattt ctggcataga aaacttcagt catttaccgg aggttccagg ggaaattaag
      841 caatttgtaa atctttataa tcaactaatc cattgttatg aatcagcgat gatacaggga
      901 ttcacatggg caaaatcgac ttatgaaatc caggattatg aacctataac agtagtaatc
      961 acagaatatg aatttaatat cttggcacaa cgttacagcg agatagagag agaacctaga
     1021 gacggaaatg atagtatagc gtatgacatt gattaccaat tacctgaagg tagtcgtagt
     1081 cgtattaatt atgagtattt aaacacaaac ttcaaaaaat acatgaatat aaaagaagaa
     1141 cgtgattcga atttaattca atcattgtta aacgacttac atcgcacatt tgcgcaatta
     1201 ccagtggagt tacagccata tgctgagatg attctaaatg atgtaaataa tggggaatta
     1261 gttatcaaag aaggatggga atttactgat tacctcaatg aatatcagct tagtgaagag
     1321 gacgatttta tccaagattt agtggattca accaatatta atgaggcatt attacgtgag
     1381 atgttggatt tacgcttaac cgaggtaaac attaatgaat atagtcgatt tgataaatta
     1441 aaatcaagtg ttaacgtagg tcatttcagt ggatatatcg agaaaaattt aggtaaaatg
     1501 gtaataccaa tcaaggtaaa tatgctaatt gacagattat tgcgtgcatt tttattagaa
     1561 gatgtattcg atgttacaga gtacataaag aattacttta actagagtac acctacatct
     1621 tatagtacat aaaaaaattc atctttttta gtttaataat tcaccaaaaa ggtgagtaat
     1681 gccatttttg aaaattgagc ttgttttatg tctacatctg tatccgtata taatccaaag
     1741 gattataaac tcagaagagt ctttattttt tctgaatata actactgtat caaaaacaat
     1801 cgattatgag acaaaacaaa atgacctctt ttatttaaga aaggaggtca ttttttgtat
     1861 cataaattgt attaaaatca atgtatcact ttttaatcaa ttttaagacc ataaattgcg
     1921 tactaaaaga aattctgcaa ttcaaaataa agcattttaa gccattttca aatttaagtg
     1981 cacccatcta cccgtcaaat cactttattt tccactccag tagtttccta attcaactgt
     2041 gtgcaaaacg atttcttcta aacaaatcaa gacaaactta taaaccttca tcccagcacc
     2101 ataattattt aaatcatagg catttttcga aattagtata tgtactcaag tttcactgca
     2161 gattatttat attttttaga gaaataaatt tttcctattg acaaacacaa agaaagcgga
     2221 tacaatacaa ttatgtcaca tgacgttata tgacgttatg taatatcttt acatttaatc
     2281 agttactttt attttggata aaattaacat ttttaaaatt ttaaggagga aatgttgatg
     2341 gatgtagcaa aaacggttaa cgcaattgta gaaaaaaatg atggagagat ttctggaatt
     2401 tatttggtgg cttgtggcgg ttcgctggta gatatgtatg tctcagatta ttttcttaaa
     2461 gccgaagcat cgaagtgtat tacaggctta tatacagcga acgaatttaa ccatgtaacc
     2521 ccaaagcaat tagatgataa atctgttgtc attctatgtt ctcatggagg aaatacagca
     2581 gaaacggtaa aagcaggaga agtcgctcaa gctagaggag ctgtgactgt tggtctgact
     2641 cataataaaa ctgcggattt attaaaggtt tctgatcata gtttccttta cgaatgggga
     2701 gatgatgcaa aaatagtcaa taatcctatg gccatcattc ttgatttaac tgtatctttg
     2761 gtcaatgtta ccgaaacatt tgaacattac ggtaaattca gagaaggcat gaaggttatc
     2821 gataccgtaa ttgaaaatgg cattaagcag gtgcaggatc gtattaaagt gtttgctgat
     2881 aagtacaaag atgaaacaat gttttatatc ttaggtagtg gaccatcata tggacatgct
     2941 tatggttttt caatttgctc tttgatggaa atgcaatggc ttgattcaac ttctgttcat
     3001 tctggagaat tcttccatgg accttttgaa gtaacagaca aagatacgaa ttttattctc
     3061 ttgatgagca ttggacgcac tcgtgcttta gatgaacggg caaaacgatt ccttgataaa
     3121 tatgcagaaa gagtagaaat cgttgatgca aaggaactag gattggatat gatatcagat
     3181 gaagtttctg aattcttcaa tcccatttta ttctacagta ttctttcaga atatcgttca
     3241 gctttagcag atgcacgcaa tcatgattta gatgttcgtc gctatatggg gaaagtggag
     3301 tactaggagg attaaaatga aggtattagg cttaggtgat aacgtcgtcg ataaatatga
     3361 aaatctggga attatgtatc ctggaggaaa ttcattgaat tttgcagttt ttgcaaaaaa
     3421 actgggacat gaaagtagct ttatgggagt ttttggctca gatgaggagg cagctcatgt
     3481 tttatcagca ataaaaaaaa taggactgga caacagtcac tctcgctttg aaaaaggcga
     3541 gaatggctgt gccagagtga agattgatga gggcgaccgg attttccttg gcagcaattc
     3601 gggaggtgtt actcgtgaat acccgattca gcttactgaa gaagacagag aatatttaaa
     3661 ccagtttgaa ttgattcata tgggattata tagccatgtc aatcatctat tagaagagtt
     3721 acaaaatgtt tctggaaaga tttcttatga tttttctgac gactttacgg aagaagaggt
     3781 tcagcgggca attgataaag ttgattttgg cttcttttct atagaagact tttccgatga
     3841 agaagtaaag cagtttttga tgaagcattt ttgtgagcga aatgatgtat tgattgcaac
     3901 ccgaggagaa aaaacggcta ttggttatga tggagcaaag ttttatgaag tgaaccccgt
     3961 gctagaagaa ccagttgata caatggcagc gggagattct ttcttaacag ctttcctact
     4021 ttattttctc gaaggggacg atatagtcac cgcgatgaaa aaggggaatg aatttgctgg
     4081 gaagtcttgt ctggttgacg gttcgttcgg ctttggtatt cattacgatt gagcttgttt
     4141 taaattcctg catttgataa cctaaagaaa gaaatcccgg taaagtggag gttaaaaaaa
     4201 tgctggatac aaatgcacaa ttaccattgt acttacaatt aaaagaaata ttaaaaaata
     4261 aaatcaatac aggggagtat cccgagaata cgaaaattcc cgctgaatca gagctgatag
     4321 aaagttatga ggtcagccga atcacagtga gaaaagcagt agaagaactg gtagaagaag
     4381 ggtacctact taaaaaacaa gggaaaggaa cttttgtcaa ccggcataaa gtttttcgta
     4441 aaattgaata tgttatgggt ttcagtgaga gttgtaaatc taatggtttt acaccgacta
     4501 gcattgtttt ggaacgcaag atcattccag caactgaagg gatggcaaaa aaactcgata
     4561 tcgctcaagg tgatgatgtt ttatatatcc agagaaagcg aatggcggat aacatcccca
     4621 ttatgttaga aaacaattat ttttcttatg aaagatatag tttcttaatg gaggaagagc
     4681 tgactgattc tttgtatgaa gtacttaaag tccaaggtac cttagccatc aatcctgggg
     4741 aaactgcact tgaaattgtc acagctgatg accaaattgc caaaataatg gaagtagctg
     4801 ttgggacgcc ctttttcttt atggatactt taatcaacga tcaaaacaac ataccaattc
     4861 atgtaggtaa tcaatattac ctgggagaag tatatcgttt ttacttatga tttcctgttt
     4921 tgatttcaag gagtagattt tatttatgaa gcaagtattg cggtcaaaaa tgatttttga
     4981 tggagagcag ccacctttca aaggttatat tgtaatcaat gaagggatca ttgaaaaggt
     5041 ggaaaacaac tggaattaca gtgatctatt aaatgaaaac acggaattaa ttgcttatga
     5101 tgatcaattc gttatgccag caattcatga taatcatgta tttttcagtg gttatttggc
     5161 tatgaatgct ggcatagatt taagcgatac atcttcagct aacgaggctg tagagcgtat
     5221 taaagctgta atgaaccata aaaaagcaga tgaaccaatt tatgctcatg gctgggatgc
     5281 tgccctgtgg aaaactgacc catcagaaga gctattgaat gaaataaacc actttggtcc
     5341 aatcacagcg atcgacaaaa atcgcagcca ttgttggatg aatgctattg ctaagcagac
     5401 ttatggtttt acagaaaaag aaaccagtgc tgaaagtcga gtattattaa ttaaggaaat
     5461 gctagcaaac aaaaagcttt tagcagaggt ttacgctgaa tttgaagcat tactgctttc
     5521 aaaaggtgtt atatcaatga aagaaatcgt ttttgatgac gcagaatttc tttctaatat
     5581 acctcagagg aaaatactta gtaacctata tgtgcaggca gtccaagaac ctctacagca
     5641 tgaacttcta tatgagtata aggaaaaagt tttccctgaa aaggtccgct ttggtggagt
     5701 gaagatcatg gtagatggtg ttgttgcgga tagaagtgga gatatttacg gtgtttatca
     5761 gtcaggagta gcttcacccg agattgacta tgaggcaatt aaagcagaag ttgctgcatt
     5821 caatgaagta gagattcctt gctgtttgac aacggaagga gataaagcgg gttataaagc
     5881 tgcgtcaatt cttgtcgaat ttggaaagcg gttacctgaa ggcgttttta attcgattag
     5941 tgatttggaa atggttactc aaaagacagc agaaaaaatg aacgaaggac aagtagtagc
     6001 agaggtatat ccacaaattc tcggcttgaa tcaaacgtta gaagaggctt acatgtcaac
     6061 tgttataaaa aat
//
LOCUS       DACJGB010000120         6020 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.125, whole genome shotgun sequence.
ACCESSION   DACJGB010000120 DACJGB010000000
VERSION     DACJGB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.125"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(60..362)
                     /locus_tag="IYI34_002825"
     CDS             complement(60..362)
                     /locus_tag="IYI34_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560642.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(582..948)
                     /gene="ssrA"
                     /locus_tag="IYI34_002826"
     tmRNA           complement(582..948)
                     /gene="ssrA"
                     /locus_tag="IYI34_002826"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1119..1892)
                     /locus_tag="IYI34_002827"
     CDS             complement(1119..1892)
                     /locus_tag="IYI34_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAR0560643.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2036..2386
                     /locus_tag="IYI34_002828"
     CDS             2036..2386
                     /locus_tag="IYI34_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAR0560644.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2367..3083
                     /locus_tag="IYI34_002829"
     CDS             2367..3083
                     /locus_tag="IYI34_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560645.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3083..4531
                     /locus_tag="IYI34_002830"
     CDS             3083..4531
                     /locus_tag="IYI34_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0560646.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4602..5111)
                     /locus_tag="IYI34_002831"
     CDS             complement(4602..5111)
                     /locus_tag="IYI34_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560647.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5151..5825)
                     /locus_tag="IYI34_002832"
                     /pseudo
     CDS             complement(5151..5825)
                     /locus_tag="IYI34_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2045 a         1050 c         1170 g         1755 t
ORIGIN      
        1 cccttttata agtatggtga taaaatccgt tccgggctac cttttttagg tagcctactt
       61 taatctttat acctttctgg atcaacgaaa gtatacttca aatagtcata acgccgatga
      121 tcgctccgtg cgtctggcac atcagtcacg atatcaaaca aaaaatacac atctttcttc
      181 attctggttt ttgttgccgg aattttaaag tagttcttat tagagtaata gagattgatt
      241 aataagctat cttcgattgc taaaaagaaa acttctgaat cccacacctt ataaaaatct
      301 ttgacaaatc tattcgaagg atcaaattta aaccataatt gtgtttttcc ttccatcaac
      361 ataatgttca cctcaaaaag agtatacgaa ctaatgttct ttttgtaaag taggaatatc
      421 attgataata tggatggaac atatatatta aaaagtgttc tttttgaatg ctattttgaa
      481 tgccattttc attcatttaa cgaaaatctg cgaaaataaa aaatgctcaa acccttataa
      541 aataaggatt taagcatcta gcgaaatctg ctgaaatctc acaatggaga cggcgggagt
      601 cgaacccgcg tccaaacaca tcgccactta aaaatctacg ttcatagtca actcatttaa
      661 ggattcactt tacagcttgc cgagtgacag gcattctgta tcgctagtct gatgttctct
      721 ttttcaactt acagacggaa agttgaaacg tatcccacta aagttaggac ccttactcga
      781 gcacatgggc gatgccgaga ggatctacgc taactgtttt taggcagcta aagcgtaaga
      841 gttgttttcg tttttagcag ttatatttaa ctgtaacgtt tttacgtaga cgtaacctac
      901 gaaacgcaat tcaagctcga ctgtgcctgt cgaatccgta acgtccccgt tagctaacaa
      961 gggttacgtg tatttttcat acaaaattag tatagcatat attgggataa attcaataaa
     1021 aaagcgaatc atatcccatt atttatataa taactgcccc tttcttaaga aaaaataagc
     1081 gattttctta agaaaagagg tagcttcaaa aaaagatctt aaatcagatc aaaatacttc
     1141 aatgcgtgga caattccgcc ttctgtattt ttctttgtga taaaggtaga aagttctttt
     1201 aatccgtcat aggcgttgcc catagcgatt ttgttgtcgc aagcttccaa caaggcaaaa
     1261 tcattcggac catcgccaaa tccaaaagta ggaacttttt ccagctgaag ttctttttgc
     1321 agacgtttga cacctgcccc ttttgagatt cctttcttca ctgtatcgat cgagtaagga
     1381 ccattccgat aaaaagttag ctcaggaaaa tgttcgtgat agtattcgtc acctgattga
     1441 gataagatca gcaacatatt tactgtgttt tctttaaagc ctaaaggatc aatttccgga
     1501 acatctgaat gaatgaagtc gtaagcattg atcattgttt ggctatgacc ggtacaccag
     1561 attttctgat cgttatagta agaaagttca tgaccgttca actgtacatg atcatacatt
     1621 ttttgacatt cttctgaaga aaatgtttga gaatagattt cttcgccgtc tactcgaatg
     1681 aaagaaccat tcatggtgac cgcagaagta atgcccgcat cttccatgat agagtggatt
     1741 tctgcttctg tacgtcctgt agcgatgacg gggagaacat tgttttcttt taatgcgttc
     1801 atagcttttg ccacttcggg tgtgattttt gatttcgcat ctagtaatgt tccatcaaga
     1861 tcaaagaaag tgattgcacg ataatttgac acaaataaaa ctcctttttt attggaataa
     1921 gtttatctta acaaagaatg aacaaaagac aaacctttcg tgaagaattc ctgaacatat
     1981 tggttcagtc aacaatttag agtatactat aagaagagaa tttaggaggg aaagaatgat
     2041 ttttttcagt attggtatct tattcttaat aattggcagt ctttttcgga ttctgccctc
     2101 aaaaggaaat ctgccttttt atgggtatca ttcgcctctt gccgcaaaaa cagacgctca
     2161 ttggcggctg gctcaaaaga caagcggaaa ctggtttttt cttatgggct tactgatggc
     2221 attgatcggc tattatttaa agacatctgg gcatacgaat tattttctga ttgaaatgct
     2281 tctgctagta tttccaatta tgcctatttt tattatgaca gaaaagaaat tacagaaata
     2341 tgacctggaa actggaggga acgataatga atattttaat gattgaagat aatcaatctg
     2401 tttctgaaat gatgcagatg tttttcttaa atgaaggttg ggaagcaact ttcaagtatg
     2461 atggaaaaga agggctcgat gcctttttag aaaacccaca acattgggat atgattacac
     2521 tagacctgaa tttgcccaca ctggacggta tgctagtagg aagagagatc cgcaaagttt
     2581 caagtactgt gccgattatc atgctgacag ctagagattc tgaaagcgat caggtgatcg
     2641 gcttagagat gggggcagat gattatgtca caaaaccttt cagtcctctg acgcttattg
     2701 ctcgaatgaa agctttgcat cgccgtagtg agttggcaga agcaagagaa gaagcagtag
     2761 tttcagatga tcattttgat atcgagacta gtcatttcaa gatgaatacg aaaacacgag
     2821 aagcgtatct ggacaaccgg ccaattgaag gactgacgcc taaagagttc gatctattgt
     2881 ttactttagc aaaaaaacca cgtcaagtat tctctcggga gcagttgctt gaattagtct
     2941 gggattatca gtactttggt gatgaacgga cagtggacgc ccatatcaag aaattacgac
     3001 aaaaaatcga aaaggtcgga ccacaagtca tccaaactgt ttggggagtc ggctacaaat
     3061 tcgatgattc tggtgttgcc taatgcgtta tttgtatcag caactcttag ctttctgcgg
     3121 aatggtcgcg ttgatcttat tgatcgttgg agtttctttt acacaactga caaaacaaac
     3181 gattgaagaa aacaattacc agcaactgtt tggatatgct gaatcagttg aaaagacaac
     3241 gcaaacctat gccgatacgc tcccgcagtt tagtcaagat caggcgtttc aaaatgcatt
     3301 gctgctgaca gagcaagtac tgacagaaca aaatgtgaac tttatattta tcgataaaaa
     3361 cgaacgtgtc atttatccga ctaatacagc tttactgaat tttaccttaa cagctgatca
     3421 atggaaaaat ctgaggaatg gacgtcaaga aaaattcaca tcgaataaaa atattttagg
     3481 acaagcacag gcgacctctt acgcacttgt gccatttaat ttgaatcatg aattctatgg
     3541 agcacttgta gttagtcaac ctgcgcgaaa tatcgataac agcgttcgtc caattacact
     3601 gaacctgttc aaaggattta ttttttccag tatcgttgca gttattgcca gctatgcttt
     3661 tgcagcattc caagttaagc gtatcaatcg gttaaggaat gcgacgaaag aagtgacgaa
     3721 tggtaatttt gatgtacagc tccccgtaca tgacaaagat gaatttgatg agttagcaga
     3781 tgatttcaat aaaatgacga attcattgaa agaatctcaa gcagaaattg aagaacaaga
     3841 aaatcgtcga agacaattta tggcagatgc ttctcacgag atgagaacac ctcttacgac
     3901 aatcaacgga cttctggaag ggttagaata caatgcaatc cctgaaaatc agcgagaaaa
     3961 cgcgattaaa ttgatgaaaa atgaaacaga gcgtctgatc cgtttggtga atgaaaactt
     4021 ggattatgaa aaaatccgta cgaatcaaat cagtatggtc attaaaaaat tcaatggaac
     4081 agaaacattg aaaaatattg ttgcacagtt agaagctaaa gcagaagcag caggagatac
     4141 attgacattg aaagccgttg atgatattga tgtttacgct gattatgacc gatttgtcca
     4201 aatcatggtc aatattattc aaaacgcgat acagtttact gaaaacggcc aaatcacgat
     4261 tactttagaa aaaggctatc ttgaaacgat catcacgatt gaagatacgg gaattggaat
     4321 gtctgaacaa caaatgaaaa gtatctggga tcgttattat aaagtagatc cttcaagaaa
     4381 aaatacaaaa tatggtgaat caggattagg tttaccaatc gtgcagcaac ttgtacgctt
     4441 gcataaagga aaattagaag tagaaagcga actaggaaaa ggaacaaaat tcgctgttcg
     4501 tttgcctgac gtagaaatca aaaaagagta aaaaacaagc ggtataaaag atcatgaatc
     4561 gatcttctat accgcttgtt ttttactata aaatgattga tttagcccgc gggctctaaa
     4621 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4681 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4741 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4801 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4861 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4921 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4981 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     5041 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     5101 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa agtgaaaggg
     5161 aatagactaa gaatgagaca gcatccactg agcccaataa gagtttgggc aaatccctta
     5221 agtatatctg tgaaaaaaag atcattttgg aaatcgatgg taaaaaaagt ctgttgatcc
     5281 atcggaattg taagtccgct taccgaagtc aagatatcac ggatatctgg cagttcagta
     5341 gtctttaaga caagaaaatt gatcctttga agaagcgctt cataaactga ctggaagctt
     5401 acgatccaca ataaaaccaa gatacaacaa agaaagagag gaaagaacag ctgccagaaa
     5461 ataaaaagga ataatttggt tttagaaaga ccagtcaggt gccaattgat caattcctgc
     5521 ttgttttgcc tgactgaata gtaaaagaaa aagataaaaa ggaaggcaaa tagaaaaaat
     5581 aaactgaata tgccttttat ataaaatcgg ctagctaatt ctaaagccgg aagaagctct
     5641 gtagatgcag aagcgccaat aaatgaaagc cgatcttgga cttgattggc taaagccgat
     5701 tgcaaatggt aaagagtcag gcatgctatc aataaaaaag cagaaaagct tgaaaataaa
     5761 caatagagta ttgtctgctt tttgtaataa attaatcgag cagtagcata gcggattatt
     5821 ttcatacttt tcacctctat aaaaagtata gaaaatgaat atgaataaag tatgaaattc
     5881 ctttagtgaa atcacgaatt aaaaccagaa aatattttgt aaaaagcaag aggttgtgaa
     5941 gaagctgccc tattctatta ccgtagatgc aagcttttga acactgttta ttcgaaaaga
     6001 ggagcagcga caataatttt
//
LOCUS       DACJGB010000121         5924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.126, whole genome shotgun sequence.
ACCESSION   DACJGB010000121 DACJGB010000000
VERSION     DACJGB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.126"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(75..1169)
                     /locus_tag="IYI34_002833"
     CDS             complement(75..1169)
                     /locus_tag="IYI34_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAR0560648.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="IYI34_002834"
     CDS             1358..2119
                     /locus_tag="IYI34_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAR0560649.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IYI34_002835"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IYI34_002835"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAR0560650.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="IYI34_002836"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="IYI34_002836"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAR0560651.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4772)
                     /locus_tag="IYI34_002837"
     CDS             complement(3759..4772)
                     /locus_tag="IYI34_002837"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAR0560652.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4937..5857
                     /locus_tag="IYI34_002838"
     CDS             4937..5857
                     /locus_tag="IYI34_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAR0560653.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1180 c         1063 g         1875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DACJGB010000122         5808 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.127, whole genome shotgun sequence.
ACCESSION   DACJGB010000122 DACJGB010000000
VERSION     DACJGB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.127"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            549..1151
                     /locus_tag="IYI34_002839"
     CDS             549..1151
                     /locus_tag="IYI34_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560654.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1167..1844
                     /locus_tag="IYI34_002840"
                     /pseudo
     CDS             1167..1844
                     /locus_tag="IYI34_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2356..2652
                     /locus_tag="IYI34_002841"
     CDS             2356..2652
                     /locus_tag="IYI34_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HAR0560655.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2653..3069
                     /locus_tag="IYI34_002842"
     CDS             2653..3069
                     /locus_tag="IYI34_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAR0560656.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3089..4633
                     /locus_tag="IYI34_002843"
     CDS             3089..4633
                     /locus_tag="IYI34_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560657.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4776..5000
                     /locus_tag="IYI34_002844"
     CDS             4776..5000
                     /locus_tag="IYI34_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560658.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5015..>5808
                     /locus_tag="IYI34_002845"
     CDS             5015..>5808
                     /locus_tag="IYI34_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAR0560659.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLY"
BASE COUNT         2072 a          923 c         1233 g         1580 t
ORIGIN      
        1 tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca tactatgata
       61 cgtgtttgtt tttctgtgct gtttagtgaa tgattagcag aaatatagag agtaagattt
      121 taattaatta ttagggggag aaggagagag tagcccgaaa acttttagtt ggcttggact
      181 gaacgaagtg agggaaaggc tactaaaacg tcgaggggca gtgagagcga agcgaacact
      241 tgatctttta agttgctatc tttttaaggt caatagagta tacttatttg tcctaatatg
      301 gttttagcag tataattgac ttggtgaata ggtcatttaa gttgggcata ataggaggag
      361 taaaatgaaa aaatttattt atcgagtttt agaaaatgac gaagtggtgg ctatttttaa
      421 tgagcaacaa tatgcgcaag attttatcgc ttacgaaaag acaatttctg ataagcaatt
      481 tgaaattgaa aaagtagata tagctgattg gttattgcaa ccgagagaat tttagaggtt
      541 ggttgaaaat ggctaaaatt ggttatgcac gtgtcagtag caaagaacag aacttagata
      601 gacaactgaa agcgttagag ggcgtttcta aggtcttttc agacaaatta agcggtcaat
      661 cggtcgaacg cccacaatta caagctatgc ttaactatat tcgtgaaggg gatatagttg
      721 ttgttactga attagatcga ttaggacgaa ataataaaga attaacagaa ttgatgaatc
      781 aaattcaaat taagggggca accctggaag tcttaaattt accctcaatg aatggtattg
      841 aagatgaaaa tttaagacgg ctgattaata atttagtgat tgaattgtat aagtaccaag
      901 cggaatctga acgcaaacga attaaagaac gccaagccca aggaattgaa attgctaaga
      961 aaaaaggaaa attcaaaggg cgacaaccta aattcaaaga aaatgatcca cgtttacaac
     1021 acgctttcga tttatttttg aatggttgtt ccgataaaga agttgaagaa caaactggaa
     1081 ttaatcgccg aacgtttaga aggtatcgat caagatacaa cgtgacagta gatcaaagaa
     1141 aaaaacaatg aaaagaggga tagttaatga gtacggttat tttagctgaa aaaccaagcc
     1201 aggcattagc ctacgcaagt gctttaaaac aaagcaccaa aaaagacggt tattttgaga
     1261 tcaaagaccc actatttaca gatgaaacgt ttatcacctt tggttttggg catttagtcg
     1321 aattagcaga accaggtcat tataacgaaa agtggcagaa ctggaaactt gaatctttgc
     1381 cgatttttcc tgatcgatac gattttgaag ttgcaaaaga taagggaaag cagtttaaaa
     1441 ttgttgctga acttctcaaa aaggcaaata caattattgt cgcaacagat agcgacagag
     1501 aaggtgaaaa tatcgcctgg tcgattatcc ataaagcaaa tgccttttct aaagataaaa
     1561 cgtataaaag attatggatc aatagcttag aaaaagatgt gatccgaagc ggttttcaaa
     1621 atttgcaacc aggaatgaat tactatccct tttatcaaga agcgcaaaca cgccaaattg
     1681 ccgattggtt gatcggcatg aacgcaagcc ctttatatac cttgaattta caacaaaaag
     1741 gcgtacaagg tacattttca ttaggacgtg tacaaacacc gactttatat ctcatttatc
     1801 aacgccagga agcaatagaa aactttaaaa aagaaccttt tttcctgaat aattcatagt
     1861 tttactaata gtctttccta acattgattt aatagtcttt aaagtattct gcttcatcac
     1921 ccattaggtg atgaaaaaag aacccctttc tgatttagcg gacaaatact ttgacctaat
     1981 ctcagaagat ataaaagaaa aaataataaa atatccgatt tatactttgt ataaagaact
     2041 aaagtagtta gcaaatcatt tacagcaaat cactatgttc cacataccat tttacatact
     2101 taccttatcc acgacacatg ttgagatagt atcgaagata aagaaagttg ctccatgttg
     2161 tttttgcgag atggggaaat caaagccaca ttgacaacca tgatgaataa gcttgctttc
     2221 agtcataagc taatcctaga accacttttc aagtcagtta gccaaatcga tgaagaaagc
     2281 gaccgtgaaa gaatggacgc tattgataag ctaatggagc aactattgga agaacgcaat
     2341 acccttattg cactgatgtc caaaggtttc ctagaacctg ctctttttaa tcaggaacga
     2401 aatgttttag atagtgagat taaaaatctt actaccgaaa aaacaaacct ggtaacgaac
     2461 tctgcgagtg gggttttgcg agcaaacgag ataaaggacc tcattaatta tgtgtcagca
     2521 gataatttta atggtgacta cacggaagaa ctatttgaag aatttgtagt gaacatcatt
     2581 gtaaattcca gggatgagct gacattcaat ttgaaatgcg gtctttccct gaaagaaaag
     2641 gtggtgagat aaatggcata tattccatat ggatacaaaa ttcaagatgg agtggttact
     2701 gtcgatgaaa aggcagcagg tcaagtaaag gtattctttg agaaatacat atcaggacta
     2761 tcccttacag tggctggcga acaggcaggt attgaaaaga cacattctgt gatggggcgc
     2821 attttgaaaa acgtcaacta ccttggaaat gatacgtatc cagcaattat tgataaagag
     2881 atatttgata aagctgaaga agttagagat aaacgtgcaa aggatttagg acgagtggta
     2941 gagcttgccg ctttcacctc tccccctccc aaagaacgat tcaaaatgaa aaaggcagat
     3001 aataagatgc cagtggatcc ttttgaacga gcagaatact tatacagtct gatagaaagc
     3061 gaggaataaa gtgacagaga aaaatataat ggttattcct gctcgtaaaa gagtaggaag
     3121 tacagccgca aaagaaaaga taaagaaact tcgtgttgct gcctattgcc gtgtttctac
     3181 agaaacagaa gaacaaaatt ctagctatga ggttcaggtc tcacactata cagagtttat
     3241 aaagaaaaat aatgaatggg agtttgctgg catctttgca gatgatggta tctctggtac
     3301 gaacactaaa aagcgtgacg aatttaatcg catgattgca gagtgtatgg acggtaacat
     3361 cgacatggtt attacaaaat ccatcagccg atttgcgcgt aacaccctag actgccttca
     3421 atacattaga cagctcaagg ataagaacat atcagtttat tttgagaaag agaacatcaa
     3481 caccacggat gccaaaggtg aggttttgct gactattatg gcatctttgg cacagcagga
     3541 aagccagagc ctttcacaaa acgttaagct tgggctacag taccgatacc aacaaggaaa
     3601 ggtgcaggtc aaccataagc gatttatggg ctacacgaaa gatgaagatg gaaatttaac
     3661 tattgtcccc gaagaagctg agattatcaa acgcatctac cgagaatacc ttgagggtca
     3721 gagtctagtg ggcattggtc gagcccttga aaaggatggt attttaacag cagcgggaaa
     3781 accaagatgg cgaccagaat cagttaagaa gatacttcaa aacgaaaaat acatcggaga
     3841 tgcccttctg caaaagactg tcacagtaga ttttctgacc aagaaacgag tgaaaaatga
     3901 agggcatgtt ccccaatatt atgttgaaaa tagccatgaa gcgattattc ctaaagagtt
     3961 atttttgcag gcgcaagaag aaattcatcg gagaagcaat atctacacag gagaaggcaa
     4021 gaacaaaaga atttacagta gcaagtacgc tttaagtgcc atcaccttct gtggagattg
     4081 tggcgatatt tatagacggg tctattggaa tatccacgga agaaaagaat ttgtctggcg
     4141 atgcgtgact agaatcgagc aaggtcctga agtttgtaag aaccgaaccg taaaagaaga
     4201 tgaactttat ggtgcagtaa tgactgcgat taataagcta cttgcaggtg ggaacaatat
     4261 gataaagaca ctggaagaga atattcatgc tgtgattggc gaaaccacag aataccaaat
     4321 ttcagagatt aacaactcac tagaggaaaa acaaaaagaa ctaatcaagc tggcgaacaa
     4381 gggtcaagac tatgaccact tagcagatga gattgatgag ctgagagaca agcgacagat
     4441 ccttttagta gaagatgcct ccctcagtgg cgagaacgag cgaatcaatg agctgattga
     4501 atttatccgc aagaacaaat tccgcacctt agaatatgat gataagcttg taaggaagat
     4561 aatccagagc gttacagtct atgaaggcca cttcgtcata gccttcaaat ctggcatcga
     4621 aatggaaata tgaaaaccag aagtaaatag cccatgactc tacagtagag ttgtgggttt
     4681 tcttttgctt gctgattata ataatatttt aataactatt gtgtttatca accaaatggt
     4741 ttataatatt cttagcgtaa acttggaggt gctgtatgac tacggcagaa atgattaaag
     4801 aactgtgtga gcaaatgaat ataagtgttt ccgaacttgc tagacgtatt ggccagactc
     4861 cacagaattt caataaaaaa ttacaacgag aaacagtaac cttggatgag ttgaaggcca
     4921 ttgctgatgt gctaggtgtc aagtttgtgc aggcatttat tttaccagat ggagaagaaa
     4981 taaaaatatc taacgaataa aggaggatgt ttatatggta ggtaatgtta ttcaaatagt
     5041 aacagagaaa ttatcatctt tgccttttat agaaggcatc gtattagggg gctcacgtgc
     5101 aagaggcacc catacagaaa attctgatat agatatcgga atctattaca attcagactc
     5161 atttgacctg acagcgatta atcaaattgc tacagagttg gatgatgaga atagaaacaa
     5221 ccttgttgta cctcccggag cgtggggtga ttgggttaat ggcggtggat ggttagttat
     5281 aaacgggtgt catgttgact tgatattacg tgatataaaa cgggtggaac aaataatcaa
     5341 agatacggag caaggaattg ttactgccaa ttatcagacc gggcatcccc atggttatat
     5401 aagtgctatg tatcgtggag aattagcgat tagtaaaata ctatatgcta agaacgaaag
     5461 cttatgcgaa ttaaaaaagc aggcagaaat ttaccctact gctctaaaga aaagcttgat
     5521 gaactttttt atatttgaag ctgaattctc tttaatgttt gtaaaagcaa atgcgggggc
     5581 agaggataaa tattatattg caggccatgt ttttcgtatt atttcatgct taaatcaagt
     5641 actatttgca tgtaataatg cttattgcat taacgaaaag aaagctataa aactgcttga
     5701 aacttttgaa tataaacctg aaaaatatgc cgaaagagtt aatcatattt ttgaagtact
     5761 cggtctttct ctttttgaat gctacgacat gaccgagaaa ctttataa
//
LOCUS       DACJGB010000123         5769 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.128, whole genome shotgun sequence.
ACCESSION   DACJGB010000123 DACJGB010000000
VERSION     DACJGB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.128"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(1014..1304)
                     /locus_tag="IYI34_002846"
     CDS             complement(1014..1304)
                     /locus_tag="IYI34_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560660.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1646..1942
                     /locus_tag="IYI34_002847"
     CDS             1646..1942
                     /locus_tag="IYI34_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560661.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="IYI34_002848"
     CDS             1949..3901
                     /locus_tag="IYI34_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAR0560662.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="IYI34_002849"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="IYI34_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAR0560663.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="IYI34_002850"
     CDS             complement(4797..5489)
                     /locus_tag="IYI34_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAR0560664.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DACJGB010000124         5697 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.129, whole genome shotgun sequence.
ACCESSION   DACJGB010000124 DACJGB010000000
VERSION     DACJGB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.129"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            127..579
                     /locus_tag="IYI34_002851"
     CDS             127..579
                     /locus_tag="IYI34_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560665.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IYI34_002852"
     CDS             593..1282
                     /locus_tag="IYI34_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560666.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IYI34_002853"
     CDS             1407..3758
                     /locus_tag="IYI34_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAR0560667.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IYI34_002854"
     CDS             3838..4233
                     /locus_tag="IYI34_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560668.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IYI34_002855"
     CDS             4558..5175
                     /locus_tag="IYI34_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560669.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IYI34_002856"
     CDS             5216..5515
                     /locus_tag="IYI34_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560670.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
BASE COUNT         1990 a          758 c         1149 g         1800 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgata
//
LOCUS       DACJGB010000125         5648 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.130, whole genome shotgun sequence.
ACCESSION   DACJGB010000125 DACJGB010000000
VERSION     DACJGB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.130"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(114..1889)
                     /locus_tag="IYI34_002857"
     CDS             complement(114..1889)
                     /locus_tag="IYI34_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAR0560671.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(2102..3004)
                     /locus_tag="IYI34_002858"
     CDS             complement(2102..3004)
                     /locus_tag="IYI34_002858"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAR0560672.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            3283..5628
                     /locus_tag="IYI34_002859"
     CDS             3283..5628
                     /locus_tag="IYI34_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAR0560673.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
BASE COUNT         1801 a         1117 c         1073 g         1657 t
ORIGIN      
        1 tagtcctcaa ggctttgttc gaactttctt tcactcttca ctataatcta aaaatagaaa
       61 atagaggaga acatcattcg tcaatgatgt tctcctctaa tcttagtatg tttttatttg
      121 gaagataaag cttttttcag attcaattta ctgaaaacta gcatgcttgt tactacgatg
      181 aattgcgtta atggcacgcc tgcgccagtg tagacactta aaaaataatc ctttttttct
      241 ggcgcataag aacaaaacca cgagacgccg aaaagaaaaa ccaattgtgt cagtattaat
      301 ccggtgacta caatcaaaga ggaactataa taggcacgat cagcttttgc aaatgcggtt
      361 cctagtatca taagtaatat ccctgctatt gaagcaatca gcacgggaat ggccagaact
      421 tggtaaattt ttatcatgac attcgcgact tttgctgttt ttgtcaactg gaaatcaggt
      481 gttctcggat cactgctatc gatccaatta tccattagtg gaagattcaa taattcttgg
      541 gcctgttcga cttgttcttg cgtaccgaaa ctataattcc ccccttgatg gaaatctttg
      601 tatcgccatg cataggcata tcctgatttc agaaaagcaa atacgattgg cagatcattc
      661 atcgtctttc catcccctga tcccatcaga tagaattctt ttttcttttt gatagttccc
      721 ttttgatagg cttcttctaa ttcttgattg acttttttcc aaaaatcatt ggtttctttt
      781 gcattgtttt ggtaataacc agattcgctt accacatctc gcaatgccca aaaaatgata
      841 tctcctggta attcttttac ttttccacct tgggtccacg catgagtcgt atacaattcg
      901 tcaatacgag tagaaacagc atgaaatgta ggagatgcgt ctaatgcttt atttaatgca
      961 gcatgcgaga cccatatctc actgttttct ttatttaagt tcgtttcgtc atctattcgg
     1021 atcagctgtt tcatcaaacg tccaaattcg ccactcgaac ggtcattgat ggcggcaata
     1081 ctatatgttg tctcgttttt atattcgatc cactgataga cgatggagaa accgactaga
     1141 gggagtaaaa acaatgaaag tttcagccat tcttgtttcg aaaggagaaa tggtcgttct
     1201 tttaacttct ttgcttcttt gaaagaaaag tcttgtccta gcaagatctg gacgaaactg
     1261 atgcctaacg cgactacagt aaaaggaaga agccagatgg aatcttcgcg aatgaaccaa
     1321 aagaaaggaa aagaaacagc tagccctaaa aaccacggca gcattttttt gctggaacta
     1381 tatttacggt aataaatagc tagtaaacaa gcgataatga tctcaacagc cggaatcacg
     1441 agggcattac gatacgttcg agtggaatat tcatgtgtaa aactaatcgg agaaaacaaa
     1501 aaataaaaat agaaacttaa aagaagatac ttgttcttca cgatcggttt aaacgcgagt
     1561 gcaaccaata tactggcaaa aatactgaac atgccgaaca aaactgcata agaaagatgc
     1621 agtttatgtg caagtactaa aaataagctg taggagattc ctttggaaag tgttgtagtc
     1681 gtatactccc ctagccattt accgtccaaa atattttgag catagcgaat cagcagctgg
     1741 tcatcatagg ttgccgacat gtctgttcca tagattgctt tactatataa atacaaacga
     1801 ataattgtaa ataatagggc tatgccaaga ataaagtatg tatgtttctt ttttagttgg
     1861 ttgatcaagg gttttaaagt gttcagcatc ttttcgtcct tccaatcacg ttttgccact
     1921 tgcttgtgaa acttttcgtt tttttacttt tctaccagta taccctgttt gtctaaattt
     1981 aaaaagaggg gggagaagat gcatgatgaa aaagactgca aaaagcactc ctttgtttct
     2041 taaacaaaaa aggacaaggc aatgtgcctg gtcctttttt gctgctgtca gatagaaaat
     2101 cttagagtat cacttcttct gctaataact caggaaacac ttctggatcg aatatttgtg
     2161 ctttgaacat ggcgatttct tttccatatg gtgcgtaagc tttcttttta tctttgccga
     2221 catagggagt ttccaagatt ttcgaaatat tgtcaaacat tggatcatgg acgatcttat
     2281 tcagcgtgtc gaatccgatt gttccaaaac caatattggc gtgccggtct ttatgcgaac
     2341 cttgcggatt tttagaatca ttgatatgga gtactttaag ccgatctaac ccgatgatct
     2401 tatcaaactc ttccattaca cctgaaaagt cttctttaat attataaccg gcatcattcg
     2461 tatgacaggt gtcaaaagta acggataatt tttcattcaa ttttactcca tcgatgattt
     2521 gcgctaattc ctcaaatgtt cgaccgattt ccgtaccttt acctgccatt gtctccaaag
     2581 cgatttgtgc cgtctgttct tttgtcaaaa cttcattcaa gccttcaata attctagcaa
     2641 ttccggcttc tgttcctgcc cctacatgag cacctgggtg aagtgtaatt tgagtagcgc
     2701 ctaaagcttc cgcacgaaca atctcttctc tcagaaattg gacggcaaaa ggaaacattt
     2761 ctggtttgat cgtattcccc aaattaatga tataaggggc atgaacgacg atatttgtta
     2821 gaccgttttc tgccatgtac gcttttccgg catcaatatt catctcttcg atggcttttc
     2881 ttctagtgtt ttgaggggcg cctgtataga tcataaaagt cgaagatcca taactggctg
     2941 cttcttctgc tgctcctaac agcattttct ttccgctcat gctgacgtgt gagcctaata
     3001 acatggatgg attcctcctt gaaaggcaat tgttactaac aattcctata tattggtaag
     3061 atgatataca aagaaaatga ttgatttttc tttgccctgt tctccatagt aactgaaatt
     3121 ttctctaaag aaaagcttaa gaactctttt ataaaaaaga tcattttctc acgttttaac
     3181 tcttttaaaa aagaaattcc tgccaaaaac agccttgtct gtttttttgt tctattattt
     3241 gctataatta gcgaaataaa accattagtt gaggtgaccc ttttgccacg caaaaatact
     3301 cgaaaaaaac agaatagaag aaaaaaagaa aaatggtttg ttcctaagat tatttttaga
     3361 gtttttcaat cattgactgt ttttattact gttcttcttt tgatgttcgc agctctaggt
     3421 attggaatag gcgctggcta ttttgcctat ctcgtcgaag acacacaatt gccgactaaa
     3481 aaagcactgc agacagaact aggaaacatt acagaaacgt caaagattgt ttatgcagat
     3541 aatacagaga tatcaaaaat ccagacagat ctgatgcgca cgactatttc atctgataaa
     3601 atttcacctt tattgaaaac tgcaattatc agcacagagg atgaatactt cgataaacac
     3661 caaggttacg ttcctaaagc tgttttacga gcattagtat ctgaagctac tggtattggc
     3721 tcttctggtg gatcaacttt gacacagcaa ttagtcaaac agcagatcct tacagatgaa
     3781 acaacattca aacgtaaagc aaacgagatc attctttctg cacaagtaga aaaatacttt
     3841 tcaaaagatg agatcattgc tacgtatttg aatgtctcac catttggtcg taataataag
     3901 ggacaaaaca ttgctggagt ccaggaagct gcaagaggaa tctttggtgt agatgcaaaa
     3961 gatgtgacgc tgccgcaagc tgcttatatc gctggattac cccaaagccc gatcacgtat
     4021 agtccataca cgaacacagg tgcgctaaaa gaagatcttt ctgccggcct tgccagaaaa
     4081 gattttgttt tattcagtat gtatcgtgaa ggccaaatta cgaaagaaca atacgaagaa
     4141 gcaaaagcct atgaccttac aaaagacttt ctaccacaac aaatcgcaga acagactgac
     4201 agagaattct tgtattatac tgtgatgaat gaagcaacaa gaatcattgc acaacaattg
     4261 gctgaaaaag acaacgctga tatgtctgat tcatcggtca gtgacgctta ttatcaaaag
     4321 gcacagcaaa cgatccaaaa taaaggatat acaatccatt cgacaatcga taaagatgtc
     4381 tatgctgcat tgcaaaatgg cgtagaaaat tatggataca tgttagatga tggtgctggc
     4441 agtcaagtag aaaccggaaa tgtattgatg gataacagaa ctggccgcat ctacggattc
     4501 gttggcggac ggaattacag tcagaatcaa aataaccatg ccttcgatac agaacgtcaa
     4561 gctggctcat caatcaaacc agttcttgtt tatggacctg caatcgacat ggggcttatc
     4621 ggatcagaat cccgtgtatc agattatgca acaacttggc aagaaggaga aaatgctggc
     4681 gagaaaatcg tcaatgctac taacgaagga tcgaacacct tccaaaccgt ccgtgagtca
     4741 ctagaatggt ccaacaatat cccggcttat cacttatatc aagatgtgtt gaattccggt
     4801 ggatcgaaac aatatgctta cgagcactac ttggcaaaga tgaactaccc ggcaaatgac
     4861 aattggggcg ttgaatctgc tcctttaggt acagtagatg tcacgacgct tcaacagacc
     4921 aatggatttc aggctttagc aaacggcggc gtctatgaag aaggctatat catcgattcc
     4981 atcacagata acgcaggaaa tgtaatttat aaacacgaaa gcaactctgt ccggatctat
     5041 tcggaagcaa cggcatcgat catgaatgac atgatgcgca gtgtgatcaa tgcaaaaatc
     5101 actacaccat tcaaagatgc catctcaagc ttaaacggaa atctaggaaa agcagattgg
     5161 gtcggaaaaa ctggttctac caatgaatac cgagattcat ggctaattgt ctctactccg
     5221 tctattacca tcagtagctg ggcagggcac gatgacaata ctggaatgga cagcaaagca
     5281 aggatacgtt cggctaatta tcttgccaat ctgatcaatc aagcttacca agcaaaacct
     5341 gacatttttg gcactgatga gaaattcgaa ttatcatctg atgtgatgaa gaaaaaagta
     5401 tctggattta ctggtcaaac acctggaaaa gttactgtca ataaaaaatc catccaaaca
     5461 ccaggaaaaa cagttgaatc gctatgggcg aaaaatggac cgaagaaaag cgcctttaaa
     5521 tttggcgttg gcggtaccga tgaaaactat acagattact ggaacaccgc ttcagcttat
     5581 gcaagagcga atcaagcaac caaagaatca gatgagaaag atgattgatt taatagaata
     5641 cacagtaa
//
LOCUS       DACJGB010000126         5598 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.131, whole genome shotgun sequence.
ACCESSION   DACJGB010000126 DACJGB010000000
VERSION     DACJGB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.131"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..2096
                     /gene="acm"
                     /locus_tag="IYI34_002860"
     CDS             <1..2096
                     /gene="acm"
                     /locus_tag="IYI34_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAR0560674.1"
                     /translation="HVYADAGRDISSNVTSLTVDPTNITDGGNIKVKFSFDEKKQNIQ
                     PGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVFNENIKNLDSVE
                     GWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSVFYYKTGDMLPE
                     DTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLGEQKVYRGEEGI
                     QQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPEQINFENHSEAW
                     FKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIGIPNVTFELRRA
                     DEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPLSSNELKFSINE
                     NDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNWEPVPDAETKRL
                     DNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENGLVVTNENKEVI
                     SVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYRFTNLPKYRDGQ
                     EIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNFPEKTEREKIRN
                     LFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKKSSFAVIIGLLI
                     VTICAGIFLQKYKKV"
     gene            complement(2368..2853)
                     /locus_tag="IYI34_002861"
     CDS             complement(2368..2853)
                     /locus_tag="IYI34_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAR0560675.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            3333..3974
                     /locus_tag="IYI34_002862"
     CDS             3333..3974
                     /locus_tag="IYI34_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAR0560676.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            3993..4628
                     /locus_tag="IYI34_002863"
     CDS             3993..4628
                     /locus_tag="IYI34_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAR0560677.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4646..5461
                     /locus_tag="IYI34_002864"
     CDS             4646..5461
                     /locus_tag="IYI34_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAR0560678.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1970 a          912 c         1155 g         1561 t
ORIGIN      
        1 ttcatgtcta tgcggatgca ggcagagata tcagcagtaa tgtcacttcg ttgaccgtgg
       61 acccaacgaa tattacagat ggaggaaaca taaaagtaaa attctcgttt gacgaaaaaa
      121 aacaaaatat ccaaccaggt gattatcttt ggatcaattg gccaagtgaa ggaaatattc
      181 gcggtgaagg attccaaaaa gaaatcccat taatgattga aaataaaaat gttggaactt
      241 taacggtcag aaaagattct gcgcaggttg tgtttaatga aaatattaaa aacctagact
      301 cagtagaagg ttgggggcaa tttgagatac aggccagaaa cgtaaccgat accggtgaag
      361 aaaatacggg accattcacc gtgacgagcg gtgataaaac agctactgta aatgtgacaa
      421 agccagcttc tggttcttct tctagcgttt tttattataa aacaggagac atgctacctg
      481 aagatactaa gcacattcga tggtttttga atattaacaa taacggcact tatgttgaac
      541 aaccagttaa aatcagtgat gaaatccaaa gtggacaaag attagatcca agtacgtttg
      601 aaattaatca aatacactta ggtgaacaga aggtttatag aggagaagag ggaatccaac
      661 aatttttaca agatttcccc agcgctacat ttaatttttc agtaactgat aattacattg
      721 aaataactat accaaaaaac tttgtcaatc ttaggaaaat aatggtgagt tataagacga
      781 tcatagaaaa cccagaacaa ataaattttg agaatcattc ggaagcttgg tttaaagaat
      841 ttaataaacc tgctgttgat ggagaaagtt tcaatcatac agttaaaaat atttcagctt
      901 ctggtggagt caacggtaca gttagaggcg aattgaagat tttcaaatat atcaatgaca
      961 cagagattgg tatcccaaat gtgactttcg aattaagacg agcagatgaa cagcctattc
     1021 aaggacagtc tagtatcctg ttgacttcga atgaacaggg agaagctagt atcaaaggcc
     1081 tgcaagtagg agactatgtt gtaaaagaaa aagaagcacc caattggatt gattttgatc
     1141 cactaagttc aaatgaactc aaattctcca taaacgaaaa tgatacagag ggagtcagtt
     1201 tacctattta taacaagaaa aaggttacga acatcacagc aactaaaatt tggaacggtg
     1261 gaaccactcc aagaccgagt atttatttta aattattcag agcaagtaca aacaattggg
     1321 aaccagttcc tgatgcagaa acaaaacgat tagacaatgg aataacttca gtgacttggg
     1381 aagatatcca acagtatgat gatagtggaa acgaatatac gtttaaagtg caagaagtag
     1441 atcaaaacgg aaatgattat gtcccttcag gttatcaaaa aattgaaaac gggctagtcg
     1501 ttacaaatga gaataaagaa gtcatttcag tctcaggaca aaaaacatgg gaagacaatg
     1561 agaaccaaga cggcaaacga ccgacgacga tcacagtaaa tctattagcc gacggtcaac
     1621 cgatccaaca caaagaagta agtgaaaaag atgattggcg ctatcgtttc acaaatctgc
     1681 caaaatacag agacgggcaa gaaatcatct atactgtaac ggaagacaac gtgcccgaat
     1741 acagtacgac gattgacggg tataacatca aaaacagtta tacaccggaa aaaacaaata
     1801 tatctatacc aggtagccat ataacaccgt ggaaaaattt tccagagaag acagagagag
     1861 agaaaattag aaatctattc tctcaaaaat tgcaaatatt tgctcataca ggagaatcca
     1921 gcgaacaaag aagcaattat attagtaaga gagctgtacc taaacagata acaaataaat
     1981 caaaaagcat tcttcctaaa acaagtagta aaaaatctag ttttgctgta atcattggtt
     2041 tattgatagt aactatctgt gctgggatat tcttacaaaa atataaaaaa gtttagttag
     2101 aatataatat tttttgttaa atatgactga aatcaaaaga ataaaattcc gatttataat
     2161 cgaaaaaaga agcattttta tatatgcttc ttttttcgta gaatcaataa atatggattt
     2221 ggctaactat ggaagaatac tcgtattagt tttcagcgtt catactagtt gttacattgt
     2281 ttgattggcc aaaagaattt ctgatatatt ttgtgctaaa taaagaaaaa gacgcttgaa
     2341 ttgggaaata agaaaactac tctatcatta caaattttca taaactggca gatgttctaa
     2401 gtaaacaagt atcttttcaa agtcggaaaa atccataatc aacgtagtgg tattgatatt
     2461 tggatgaaag cctacggtat cttgacgatc aatagcagca tcgatcacga cttggatttt
     2521 tttctctttg tcgtgcatca atgctaatgg agtcactgta cctgctggaa cgcctaggag
     2581 gtcaggaagc tgttcttcag aaacaaaaga taagcgcttt tcttttaatt gttcagctag
     2641 ttgttttaaa tcggcgtgct tttcatcagg caagatgaca agatagattt gttttccttt
     2701 tcttgatttc aataatagat tttttacttg cggaccaggt agggaaaaag ggacattttt
     2761 gactgacgta atcgcaggat gatcgacttt ttgaaaagag atacccagtt tttttagtag
     2821 ttcataagct tcttgttcgg ttgcacgact catcgcgcat caccctcctt tttttattag
     2881 tgtatcataa aaaagagttg acaaatgaaa aataatcatg taaattacac gtaaagttaa
     2941 aacaaatgac atcgaaaaga agagtagccg ttaggtgttt ttagagagtc tctggttggt
     3001 ggaaagaggc agcagttaac cgtgaaacac atcttggagt cgattttctg aagcgtatag
     3061 taggaaaatc cggggagccc gttacagctg cggtcgttgc tagatgacca gataaggctg
     3121 gcttttccgt gaggagtcgg taaaaaaagg tggaaccacg tgattaacgt cctttgatct
     3181 tcggatcaaa ggactttttg ttttcatctg aacggcaacg actgtgtgag gttttttctg
     3241 caaggaaaaa ataaacagag gtggaaccac gtataatacg tcctcttggc atttgccaag
     3301 aggatttttt tattttaaag gaggaaaaga agatggagta tttgattgaa gttatgccgc
     3361 aattattagt aggagcactc acaacattga aattattttt atttaccttg attggctcat
     3421 taccattagg gattgtttta gcattaggat tgtctttgca ttttaagccg ctacgctact
     3481 tgcttcaaac atatgtatgg gttatgcggg gaacaccgct gttgttgcaa ctgattttta
     3541 ttttttatgg gttgccgacg attgggatcg tttttgatcg tttcgaagca gcactcattg
     3601 cctttatctt aaactatgca gcttattttg cagagatctt tcgtggaggg atacaatcga
     3661 ttcctcaagg tcaatatgaa gcagcgcaag ttttgcaatt aagtcccttt caaacggtga
     3721 cgaaaatcgt catcccacaa gtgacaaaga ttgttttacc ttctgtaggg aatgaagtta
     3781 tcaacttggt caaagattct tccttgatct atatattagg tttaggtgac gtaatgcgtg
     3841 ctgggaaaat cgcgatggaa cgcgacgcga ctttactgcc tttgctaggc gtggcaatca
     3901 tctatcttac attgactggt attctaacaa tcgtaatgaa acaagttgaa ggaaaattaa
     3961 attattaccg ctaactgata ggaggaagaa atatgattga attgaagaaa gtgaacaaag
     4021 catttggtga aaaaaaaata atcaaagatt tgaatctaat cattccagat gggaaaacat
     4081 tagctatcgt aggtccttcc ggtggcggta aaaccacgct tttacgtatc ttggctggct
     4141 tagaaagttc tgacagcgga gaattttatt tagacggtgt accgttcaac ccattcgagc
     4201 ttaaaatgaa agaacaagtt gtaggagtcg tattccaaga cttccagcta tttccacatc
     4261 tgaatatttt tgaaaacatt accttagcac caaaaatggt attgaaacag acaaaagaag
     4321 aatacaccaa aaaagcagaa gaattagcgg cttcattagg gattagtgat ttattgaata
     4381 tctatcctta tcagctttct ggcggacaaa aacagcgggt agcgattgcc agagcattgg
     4441 ctatgaatcc acgggtattg gcttatgatg aaccaacaag tgctttagat cctgaattac
     4501 gccagcaagt cgagcgtttg atcctatcat tgaaagaaga tggggtaacc caaattattg
     4561 tcacccatga tttagtattt gcagaaaatg tcggtgatca attactaaaa gtttccccaa
     4621 ttcattaaga aaggagaaga tagagatgaa aaaaaccagt tttctattcg ctttgatcgc
     4681 aggtcttctt ctttttgcag gctgttcgaa caaaaaaact tcagcagata attgggcgac
     4741 tatcaacgaa gagaaaaaaa tcgttatagg tttagacgat acatttgttc ctatgggatt
     4801 tagagataaa gacgggaagc tcacaggatt tgatattgat ttagccaaag cagtctttga
     4861 acaatatggg attacggtag actttcagcc cattgactgg tcaatgaagg aatttgaact
     4921 gaacaatgga acgatcgatt tgatatggaa cggctattcc aaaacgccgg caagagaaaa
     4981 gaaagtgcaa tttacaaaac cttatatgga aaacgatcaa gtgctgatca cacctaaaga
     5041 atcaggaatc acaaactttg gacaaatgaa taataagaga ttaggtgcgc aaaatgggtc
     5101 atctggttat gatgtattta cgaaacaacc agaagtatta aaagagatag tcaaaaacca
     5161 agatgctgtt ttgtatgata gttttaatga agcattgatc gatctgaaaa gcagacgggt
     5221 cgatggtctg ctgatggata aagtatacgc agactattat ttgaaacaaa gaaacgaact
     5281 agaagacttc aacataacaa aaggttctta tgagagtgag gattttgcag ttggtgcacg
     5341 caagtcagat agtacattag tgaaaaaaat caatgaagcc ctagatcaac tagagaaaac
     5401 aggtgaattc caaaaaatat ccgataaatg gtttggcgaa gatgttacgc cgagaaatta
     5461 acaaataggg gactgtgaca aaagacgtgt cacagtccct ttttccgaat aaacagtgtt
     5521 ctcctgccaa ctccttgtga ctcggagctg aatggcaggc tcacaacctc ttggtaaacg
     5581 gtgttcaaaa gccagttt
//
LOCUS       DACJGB010000127         5505 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.132, whole genome shotgun sequence.
ACCESSION   DACJGB010000127 DACJGB010000000
VERSION     DACJGB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.132"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            279..1127
                     /locus_tag="IYI34_002865"
     CDS             279..1127
                     /locus_tag="IYI34_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560679.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(1298..1639)
                     /locus_tag="IYI34_002866"
     CDS             complement(1298..1639)
                     /locus_tag="IYI34_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAR0560680.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            1725..2432
                     /locus_tag="IYI34_002867"
     CDS             1725..2432
                     /locus_tag="IYI34_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAR0560681.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2419..2742
                     /locus_tag="IYI34_002868"
     CDS             2419..2742
                     /locus_tag="IYI34_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAR0560682.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(2831..3868)
                     /locus_tag="IYI34_002869"
     CDS             complement(2831..3868)
                     /locus_tag="IYI34_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR0560683.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(4315..5298)
                     /locus_tag="IYI34_002870"
     CDS             complement(4315..5298)
                     /locus_tag="IYI34_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAR0560684.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
BASE COUNT         1609 a         1142 c          982 g         1772 t
ORIGIN      
        1 tctttgcgat acatcatacc tcttttatca attggaacaa ttatttattt ctaacaaaat
       61 cacttcattt ttgtcagata aataaaaaac aaaaaaagtt ccatctttaa aacacgctat
      121 tcttttactt tttctcacat tctctaagaa aagaaaagga gaatgaattt ttgacaattc
      181 gagcaaaagt gattatcaaa tatgtaaaat cagctacttt tttcttaaac gtttctttat
      241 actaaatata aataagtgtt tcatgaaagg agaactagat gaataattgg cttgagtatt
      301 ttcctgaaaa cgttttggaa cgcggttaca gctatcatct gcacggtttt gtccgtcatt
      361 tgaactatac aagcaagtac ttgtccgcaa cagtttctgg cacggaagat tataaggtag
      421 tgatcacttg ggatgaaaag accaacatga cttgtgattg tctatatgcc atagaaggaa
      481 aaaaatgcaa acacatggct gctgtcttat tcgcttacga agaacgtcca ataaaaaaat
      541 ccaactactc actttccgaa ctctcgtccc ttgtttcctc cgctagttcc tctcttgtac
      601 gggagctttt aacagagatt cttatagaac atccgcactt tatcgaacgc ttcaaagtta
      661 agatgccatt tcatgcaata aattattcag ataaattaac aactatcatc cataaatacg
      721 atcatattat caagaaaaac aaaaacagga aaacagcgaa gttcatcatg gagatgcgaa
      781 agttcatcca agaagcagtt gaatcgctta ttcagcaaaa tgcctatctt cccgcattcg
      841 aattgatcaa tgaagtgatt gctactctcg aaacattcta ttgggagcct gaggatgaac
      901 gaacccttct tctgatagag gactgttatt atttatggaa agaattatta gcagaagcac
      961 ctcatgcaga aaaaaggcaa atgttctctt ggttcgtctg ccaagtcgat catactgacg
     1021 cctcttattc aaaacgctac tctataaaaa tcctgaaaga agactttcgg gaaaaagaat
     1081 tttctaacca aaagaaaaaa atagataaaa aactaaagaa acggtaaaga aagacgattt
     1141 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
     1201 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
     1261 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
     1321 tcgttgctgg atagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
     1381 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
     1441 tgtcgaacca ccctaaccct agtgcagcaa tcattgttgc gcctaatgca ggtccttcct
     1501 ccacacttaa acaatagacc gttgcatcga aaatatcagc ttggatttgt agccatgcaa
     1561 gatttttcgc gcctcctccc accgaaacaa tacgtgtaaa tctgcgaccc gctagttctt
     1621 tacgtttcaa tcccaccatt gacaaatgct cattaaaaat ttagaatatt cacaagttta
     1681 acgacaatta tgaaaattta tgtcgtgcta aaggagtttt gttattggat gaaaagttag
     1741 atataaaaac tgcgatcaaa gacacgttac ctaccgtttt cggttatatc ggtattggac
     1801 ttgcatttgg tatcgttggg aaagctgccg gatttcatcc actagtcgtc acgttgatgt
     1861 ccctgctggt ctatgctggt tctgcccaat ttatcacagt cagcatgctt gctagtcaca
     1921 gcccattgct ttccatcgtt ttctcgacct ttctagtcaa ttcccgaatg attctgatga
     1981 gtatgacgat tgctccttat ttcaagaaaa atcggctgct tcaaaatctg ctgattggca
     2041 cattgctgac agatgaaagt ttcgcactgg gaatgaataa attgaactat actggacaaa
     2101 agttaaactt tcgttggatg aatacagcta attggatttc ttatttgacg tgggtgggtt
     2161 cttctcttgt tggcgcactt ttagggaatt ttattactga tccgaaaaag tttggtttgg
     2221 atttcgccat cgtcgctatg tttatcgggt tactttatct tcaagtcatt tctgatagaa
     2281 acacctctaa acgattacag ttgatcctga ttggtctcac tttgattctg gtatatgtcg
     2341 gactgatttt tatcccaagc aacttagtga ttgtagttgt aacactgatt gggtgcggat
     2401 tgggggtgtg gatcaaacat gccttctttt gagtttacat tgctgactat tttaggttgt
     2461 actttagcaa cttggctttc tagggtccta ccatttgtct tactgaaaaa attcgattta
     2521 ccgcaaccgt tgcttgagta tttaagtttt gtcccgatcg tcattatgtc tgctttatgg
     2581 ttcagcagtc tatttaccca aaatatcgga catctgccgc agatcaatct cgaaaatgct
     2641 ttggcttcag ttcctacact tttagctgct attctttcaa aaagcttatt ggtgatcgtc
     2701 ttggcaggaa ttctttccct ttcgttgatt cgcttattat aaataaaaaa acaagagctt
     2761 atggcaaatt actgtcatgc gctcttgttt ttatcaaagt caaataagaa aaaaatcggc
     2821 gtattcttgc ttatccgttg aacggaccac taacaacgac ttttcctaac atatgtcctg
     2881 tttctacgtc tttggttgct tgtttcaagt tcttcgcatt catcccatct gaatagacct
     2941 tgctcaatgt actgcggata atcccttcat ttgctaaagc agcaatgtgc gctaaagctt
     3001 ctccttgcgt ttcgatttca tagttatagt ctgttttagc gaacatatac tcccagtcga
     3061 agctgataga ttggtttttc caaacaccta tatctaacgg ctctttgatc cctacgatcg
     3121 ttcccacatg tccaaatggt cggacaaggt gcttgatttg atcaaagtag cgggtaatat
     3181 cgaataatac agcgacatac tctacttgat cgattcctaa ttcacgaaaa cttttactta
     3241 agtcgtgatg ataatcgatt ggatagtcta cacctgtttt tttcagccat tcaaaattct
     3301 tcggacttgc agtcgcgtag acttctaggc cagcccaatg cgctagttga ttcaaaatcg
     3361 aaccgacgcc cccggatccg ttgatcacta agattttttt cccgcgattg gcattttctt
     3421 ctggagttag accaaatttt tcaaataaca attcataagc ggtcaatgag gtcaacggca
     3481 aggctgcagc ttcttcatct gaaagatttt ttggtgcaag agcgacaata cgttcgtcaa
     3541 ctagttggta ttcttgattg cttcctgctc ttgttgttgt acctgcgtaa aaaacgcgat
     3601 cgccgacaga aaatttttgc acttcgtcac ctacagcaac aactttcccg actccatcaa
     3661 aacctaagat acgtaaagca tttcttatcc cgttatcttg gcgaagtttc gtatcgacag
     3721 gattaacaga tactgcgttg acttttacca gaagatcccg tgaactgggt acaggtatct
     3781 gttctttttc atcaataaag ctttttggat catcgatcgg caacccttca taaaaaccga
     3841 ctaccttcat taattccttt tctgacatac gaattcctcc ttagcttcat caagttggag
     3901 catccataca gtgttcattt caatatatat agtatgctat ttgttccacc ttttttattc
     3961 atcataatta ttctcttact taaagtaaac tatttgcact tttttggtta aaaaagaggt
     4021 tgcgacaaga cttttgtcac aacctcttat aaacggtgtt ctcctaccaa ctcttcggtc
     4081 acaagtcaaa atattcatca aacatgagaa gctgtttttt gaaattgctc cttgtgactc
     4141 agagttaaac ggcaggctca caacctctga tataaacggt gttcaatcaa ctgaccctcg
     4201 gtattagctc aaaaaacgac aaaatatgaa gagctatttt tgtttggctg ttcttattac
     4261 ttgatacagg acagcttttt cacgacctct aattcacggt gttcaagcaa gaaatcattg
     4321 ttcactgaaa agcgttggat gttctttttc aaaggtctct aagatctctc gtacttcatc
     4381 agtagatttc gtattcatca actgaactcg caagtcattt gctcctggaa atcctttgat
     4441 atagatttta aagaaacgat gtagtcctgt gatggaacgc gggatgattt ctgaataatg
     4501 atcttgcaga tctagttgca gacgaagtag tccgatcagt tctttttctg tatgttccct
     4561 cgggtctttt tcaaatgcat aaggattctt gaaaatccct cggccaatca tgatgccatc
     4621 aacgccatat ttctcggcca attcttctcc tgtctggcga tcggggatat ctccattgat
     4681 cgtgattagt gtttgtggag cgatttggtc acgtaaagca atgattttag gaatgacttc
     4741 ccaatgtgca tctactttac tcatctcttt acgcgtacgc aaatggacag aaagattggc
     4801 tatatcttga tgaagtaggt gactgatcca ttcttccatc tcagacattt ccgtatagcc
     4861 gatacgcgtc ttgacgctga caggcaaacc gccagcttta gctgcttcaa tcaattgtgc
     4921 cgctacttct ggacgaagga tcaagccact gccttttcca cgttctgcta cattagggac
     4981 tgggcagccc atgttgatat cgattccttg gaagcccatt tctgccatag caatgctcat
     5041 ttctctaaaa aattctggct tgtctcccca aatatgggca accatcggct gttcatcttc
     5101 tgtaaatgtt aatcttcctc gtacactgtc ttttccatct ggatgacaga aactgtctga
     5161 gtttgtaaac tcagtaaaga agacatccgg tgctgctgca tgctttacga catggcgaaa
     5221 tacaacatct gttacatctt ccattggtgc taaaacaaaa aaaggctttg gcagctcttt
     5281 ccaaaaatta gtagtcattt atttgttact ctccattcta tcaagcagtg aagtcgcaat
     5341 ctttctcaaa aacattgtca ttatactagg aaacaaaaaa agaagcaaag tgaacagctt
     5401 aaaagatgtt tctgattgag caaaaaaaag actgtgacaa aaatcttatc acagtccatt
     5461 ttccggaata aatggtgttc aaaagcttgc atctgcggta ataag
//
LOCUS       DACJGB010000128         5497 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.133, whole genome shotgun sequence.
ACCESSION   DACJGB010000128 DACJGB010000000
VERSION     DACJGB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.133"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..954
                     /locus_tag="IYI34_002871"
     CDS             <1..954
                     /locus_tag="IYI34_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560685.1"
                     /translation="STATPYQPNLLDDPYWLGKAPLGENIANKSVTFPIKSSAYSLYQ
                     ANMEEEFVLGQTYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTFTP
                     QKVGVNYPKRLTIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPN
                     DYSWDVTPEYTEKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKD
                     NSQSFIDGYATFIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPS
                     GTSSVSKAMYVRKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            957..1403
                     /locus_tag="IYI34_002872"
     CDS             957..1403
                     /locus_tag="IYI34_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560686.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            1405..1542
                     /locus_tag="IYI34_002873"
     CDS             1405..1542
                     /locus_tag="IYI34_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAR0560687.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            1579..1872
                     /locus_tag="IYI34_002874"
     CDS             1579..1872
                     /locus_tag="IYI34_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560688.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            1895..2092
                     /locus_tag="IYI34_002875"
     CDS             1895..2092
                     /locus_tag="IYI34_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAR0560689.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            2103..3122
                     /locus_tag="IYI34_002876"
     CDS             2103..3122
                     /locus_tag="IYI34_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR0560690.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            3379..4026
                     /locus_tag="IYI34_002877"
     CDS             3379..4026
                     /locus_tag="IYI34_002877"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR0560691.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            4309..4382
                     /locus_tag="IYI34_002878"
     tRNA            4309..4382
                     /locus_tag="IYI34_002878"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4343..4345,aa:Ile,seq:cat)
     gene            4928..>5497
                     /locus_tag="IYI34_002879"
     CDS             4928..>5497
                     /locus_tag="IYI34_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAR0560692.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1946 a          866 c         1085 g         1600 t
ORIGIN      
        1 tcaacagcca ccccatatca gccaaactta cttgatgacc cttactggct aggtaaagcg
       61 cctttgggtg agaatattgc taataagtct gttacgtttc caatcaaatc tagcgcctac
      121 agcctatacc aagctaacat ggaagaagaa tttgttttag gtcaaaccta tacaattacc
      181 atgaaggcaa ccaaaccacc tatccaaacg ttcattgttt acaacgagga tagtggagat
      241 tatagatatg gtaacttaga gccagtagaa gggttagttg atacatgggg tctgactttt
      301 acaccgcaga aggttggagt taactacccc aaacggttaa ctattatcca gtatccccaa
      361 tcaacaacag gtgcatgtca aattgactgg ctcaagatcg aaaaaggcga cacacgaacc
      421 ccgaatatta gtcagtttaa atactttggt gaaggcttga aagacagtaa caatcccaat
      481 gattacagtt gggatgtcac acctgaatat actgaaaaag gcttgaatga tgctgttaat
      541 gtgtatgatc ctcagagagt tgaaggtttg aaaaactttg ccgatggtat tcaaatagca
      601 ggagataaag tgatcagtga aaatgattgc actgtttata cattaactaa agacaacagt
      661 caatcgttta tcgatgggta tgcaacattt attaaacacg gaaaagaagt tattgtaaat
      721 ggtacagtaa agttcaaaaa agcttatgct tttggtgtac cacttgatga tgaagtccca
      781 gatgaatttg tcgcaaaaat tgttcatggc atgcttacag gtccgtcagg aacaagtagt
      841 gtatcaaaag cgatgtacgt gcgaaaagac ttaggaacaa ttattacgaa tagcgaattt
      901 gctgcaaatg aatggtttac attccatggt aattattggg taggggagga ataaaaatga
      961 aaaatatttg gaaatacgga cgaacaggtg gagagtatgc tggacaagtg cttgatgata
     1021 tggttatgac tgttccattt accgatgttc caccactcga aggaattcgt gctgatgggg
     1081 aaccactaac gattgctgat cagatgtttg atcctaaact gaaccaatgg attgttttag
     1141 tgaatgtact agatcacaac gatttaaaca atctcaaagc gatgtatgag tcgttagaaa
     1201 atgagaacgg cgatttaaaa cagctcaacg ccaaactcat gctaagcgat gtagcgatta
     1261 aacaggaaaa tactgcattg aaagaaaaag cggatggttt agcacaaatc aattcaaaaa
     1321 cgatgcttgc ttcgttccaa aatagcaaag acattgcaga aattaaagag caactaaatc
     1381 cagcttcaaa gggaggtgag tagtatgttt agttttagcg atgtgaaaat gatgtatgat
     1441 tggggctgtt ttactgacga tcaagttcga ctattcgttc cactatgcat tacagacgaa
     1501 gaagcagaaa aaatcattaa taaagataag agcgcatctt aagtgatgcg tttttattta
     1561 aggtaaagga gttgtcacat gattaattta ggagaatggg gaacaatcgc aggatcaatc
     1621 actgcgattg tttctttgat tttattagta ataagaccag tcactgcatc tttctcgaag
     1681 attactgaaa ctctttcaaa agtaagtcac aatttagatt tgctgactaa agatttagaa
     1741 tcgagcaaat cagatcgatt gatgattcat gaagaactaa agaaacacga tgaaagatta
     1801 gatacacata cagaaaaatt ggtagaacac acacaacaaa tcaaaacttt atttagagaa
     1861 agatctcggt aaaaaagaaa ggagttaaga agaaatgatt ttacccgata agtattatca
     1921 agtcattaaa tggacagttt taacagtatt gccagctgca tctgtattag ttgcaacact
     1981 aggcaaagcg tatggatgga atggaacaga tatgacagta ctcactatca atgcagtagc
     2041 aacattttta ggcgttatca ctggcgtgtc ggcttataat ttgaaaaaat aggaggaaac
     2101 aaatgaaaaa gaaaattact attactgcga tgagcctatt aacggctctt tttttattgc
     2161 caattaatgg gtttgcctat acgattaaca atgaatttaa tttaggccca aacgaaggta
     2221 gctcacaagt agcaaataat cagtacattt tactgcatga aacggctaat gaaacagcaa
     2281 caggacgcaa tgaagcgcag tatatgcaac gttcatggac tagcgcttat actgcttata
     2341 ttgtgggaga cggcggaatt gtttatcaag tcggtcaacc tggttatgta cagtacggtg
     2401 ctggttcgta tgctaatgca aatagtcctg tgcagattga gttacaacac acacatgata
     2461 aagcaacgtt tgagaaaaac tacaaggcat acgttgaatt agctagagat tcagcaatga
     2521 aatatggtat tccacttaca ctagacacac cgtataatca accagggatc aaatcgcatt
     2581 tatgggtaac acaaaatatt tggggcgatc atacagatcc ttacggttat ctttctgaaa
     2641 tgggcgtaag taaagaaaaa ttagcctatg atttggctca tggatttacc gatgaaaatc
     2701 cgacaacttc agatgataaa ccagtcattg atccaactcg agcaggtgct gcaaatccta
     2761 ccctgacaga tggaacaaat tacgcccaca ttgatcagtt cggagaaatc gaaaacgcaa
     2821 acttacatgt ggctggttgg cacatcgcta actataaata cgagtatatc ttcattatgg
     2881 attacaatac tgggaaagaa ttagctcgag taaaagctga tggaatatat agaccagatg
     2941 taaatcaagc ttataatact ttaggaaatg ttggctatca tgtatctttt aacatgcgca
     3001 acttccctaa taagaaagtc tatgtaatga tgcgggcaac gaatgatcca gaagggaaca
     3061 ctaaaggcgg agcacaagat tttcatgata aacgctggta tttaaatatt ccgcaacgat
     3121 aaaaatagcc cctcgttttt gaggggaagt acataatgca aatttgtcca taacttattg
     3181 attatcttgt aaaaatgcgg taatttaata gttagtgaga agaggtgctt aattatagtt
     3241 aaaaaagagg aaatagaagc agcatgtaac caagcaaatg ttaggtttat tggagaagct
     3301 ttatctggat atcctaagta taaatactta cacaactggt tacaaagtaa aagtttttat
     3361 cttaataaag aaagatgcat gcctaataaa aattataggg tttataaaag aggaactatt
     3421 gtatatgttg attttggggt gaacgtgggt tatgagttat ctggtaatca ttttgctatt
     3481 gttttaaata ataaagataa ttcgaaaaat ggtgttgtat gtgtagtacc aatctcttca
     3541 aaaaagaaaa gtaattatgt atctgtagga aaaatattag aagttgctag tattaaacaa
     3601 tttataagcc aagctgataa attaaaagag aaacttagga taattatttt actttcatta
     3661 aatagaggca tcattgatga aagcgatttg aatagtttta ttaaagatat tgaagctaaa
     3721 ggtgaaaagc tttcagtgga agaagtagaa aaacgagctg taaaacataa tttggtttgc
     3781 gagacaaggg aacaagtaat taaagaaata aaatcattat ctgaagatat ggtaaaatat
     3841 cagaaggtgt ttaattgcta caagaaatat acacaagaat cctatgtaat gccacttaat
     3901 atccaaacga ttagtaaaaa tagaattaga agaattaaca agtttgatcc ttctggaaat
     3961 atgaaggcac ctggaaatgt cctcaatgaa gtagatgaaa gtttaaaaaa atattttaca
     4021 aagtaaacga tatttttgtt gacatttttc tgttcgttag ttaccatgta gttatagaag
     4081 gacgtaagcg cctattttta atgtacacat cgggacgtat gcgcccacat agaaacatag
     4141 ttgagagaca tctatatggt gtctcttttt ttattaatat gcaaaaaagt ctagtttttt
     4201 caataatatt tatgtaataa taaattagcc accacaacaa agaatgaaac ccattattat
     4261 ctagtctatg tccattcttt ttgtttgcag tagttgtgat ggctttccgt actcttagct
     4321 cagttggtta gagcagacgg ctcataaccg tccggtcgta ggctcgagtc ctacagggta
     4381 catactctaa gtgatttaaa ttgcgaataa aatataaaaa tgactcccct tttgactccc
     4441 ccaagcagta tatggatgtc tctaaaagtt gtttttttag ataataaaaa gctgataata
     4501 agcgaaaaac acttaaaatc agctttaaac cctattctcg aaatccggcg aaccggctaa
     4561 taccggcgcg caggttcaaa tcctgtacct tccttatatc aacgattaga ggttgtgaag
     4621 aagctgtcct gcatcaagta ataagaacgg ccaaacaaaa atagcttttc ctattttttc
     4681 gtttgattgg gcttattacc gcagatgcaa gcttttgaac accgtttgtt cggaataagg
     4741 ggctgtgaca agacttttgt cacagcccct tattgttgta cttttaacct acaatatttt
     4801 ttgattcccc ctgagtacta ttttttataa atgctagtaa atcaatcttc tttggccttt
     4861 ttttgataaa aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg ttttaggagg
     4921 ggaaacgatg tgtaccgcaa tcacttatgc aacgaaagac cattattttg gaaggaattt
     4981 tgattatgag atgtcttata atgaagtggt cactattact ccaagaaatt atcgtttcga
     5041 ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg ataggaatag cagctggggt
     5101 atccaactat ccactctatt atgaggctac aaatgaaaaa gggttgagta tggcaggact
     5161 gaattttcct ggcaatgctg attataaaga attacaagaa ggaaaagata acgttgcacc
     5221 atttgagttt attccatgga tactagggca atgctcaacc atagacgaag ctaaagaatt
     5281 attggctact atcaatctag tcaatatcga ttttagtgaa aaactaccct tgtcaccttt
     5341 acactggtta ttagctgata aagaaaaatc gatagtaatt gaaagtatga aagatggact
     5401 tcatttatat gataatcctg tcggtgtgtt gacaaataat ccgccatttg attatcaatt
     5461 atttaatctg aacaattatc gctcgctatc aaatgga
//
LOCUS       DACJGB010000129         5350 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.134, whole genome shotgun sequence.
ACCESSION   DACJGB010000129 DACJGB010000000
VERSION     DACJGB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.134"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(30..425)
                     /locus_tag="IYI34_002880"
     CDS             complement(30..425)
                     /locus_tag="IYI34_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008688205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560693.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(415..627)
                     /locus_tag="IYI34_002881"
     CDS             complement(415..627)
                     /locus_tag="IYI34_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560694.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1699..2301
                     /locus_tag="IYI34_002882"
     CDS             1699..2301
                     /locus_tag="IYI34_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560695.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2656..2892
                     /locus_tag="IYI34_002883"
     CDS             2656..2892
                     /locus_tag="IYI34_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAR0560696.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2889..3302
                     /locus_tag="IYI34_002884"
     CDS             2889..3302
                     /locus_tag="IYI34_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAR0560697.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3821..4771
                     /locus_tag="IYI34_002885"
     CDS             3821..4771
                     /locus_tag="IYI34_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HAR0560698.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4768..>5350)
                     /locus_tag="IYI34_002886"
     CDS             complement(4768..>5350)
                     /locus_tag="IYI34_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560699.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1913 a          764 c          903 g         1770 t
ORIGIN      
        1 caacagaacc tttgtatgaa cctttatttc tacatttttt tctcaagcac actactgata
       61 atgattaaaa taatcggctg gattgcaatg atacttccga caataatagc tggaatgatg
      121 tagtctaaac aaataaatat aactgttgcc acccctaaca gtattgtgtt aaacatattt
      181 gttatatttc ccgctttttc tttgattatg atattccgtt cgtccataac attgattttg
      241 tagtctgcat catttttaat cattttatca tgcaaaattt gaacatgaga aacgccaaaa
      301 acggcactga ataaaatagc aagtccaata tctaccattt taggaaaatc tgtaaccaaa
      361 attgtcacaa tcaaaaacaa cgaaacaata taaccgatat accaaatctg acccttattt
      421 ttcatcttca ctttcctcct catagataaa aatttcttca atgctcattt gaaagtacct
      481 tgcaatttta aatgccaata taatagaggg attataacgt ccattttcta aagaacctat
      541 tgtttgacga gatacttcaa gagcagaagc taaatcttct tgtttaattc ctctttgttt
      601 tcgtaattct tccaaacgat ttttcacaac atgctccttt ctaaaaaatg gaaagtttac
      661 tttcctttcg tgcttttata atactcctgc gagcacaaaa tgtcaaggaa gctttccata
      721 taaattttga ttttaatgtt ttagttactg tttttatgac acatgaacac acttattctt
      781 atttcctctt atctgctatc aacagaaaag aaaaagccct gtcaagagcc gattagatca
      841 agccagtcga gaaacgatgt tagaccaaat tgattttggg taatgaatgt ttagaactta
      901 gtagtcatcc aaaaacgact actttttttg ttgaatcgtc caaggacgat tcgatgcttt
      961 tgacttgtaa gaacaggatt gaatgaaacc atactgttcg catattttta gtggatgaac
     1021 aaacaaaata cgagagattt tttgttcgtt catccatggt tttagaaaaa agagggacga
     1081 ttttggaaga agaaaatcgt ctcttttttt tcttcttttt gtatgacaaa aagaaagatc
     1141 ttttgcccat tttattttta taaaatgggt aggtggcgtt cgcgtaaagc aaatcgatac
     1201 aatccaatgg ttttggcgaa tcagatttac cattctaggc ttgtttaata cagacatcga
     1261 ttgttatttt gagaatttat atccttattg gcaatgaaat ggatattctc cataccaata
     1321 gaaatcttca agataatttt cggaaatata taagagttaa tcaattatga tcatatagta
     1381 ggtaaaaatt aggacagcct tcaattatat gggaaaccaa tataatcaag taattattcg
     1441 ggtaacacaa atactagagt actcatttct tataactgtt ttacacctat tagggatcag
     1501 tattattaat agatctagat aaattaatac gtaattatat aataatttat gtttgtgcca
     1561 ttaaacaatc ttaacagata tttcatttta tttatgtaaa acaacacgaa taattattgc
     1621 ttcgtaaagt tatatgagta taagttaatt tactggaggt agtgcaatgc ttttttctga
     1681 aagatttggt tttaaaaaat ggaggatatc atactgagag atagattgac agaccctctt
     1741 aaaaatagtt tagcaaatgt gatttatgat atattacgag aggctgataa agtttatagt
     1801 aattataatg gaaaagtctc atgggtaata tattcttttt taattaaaga gttttttaaa
     1861 gaacagataa ataatagcta tgagttgggt tatcatttta ctgtttatta tgaagatatt
     1921 atcaagaggt tagaagactt ggagtggtat gcttattatg atttattaga ggtttttgca
     1981 agtgctaaaa tcttggataa aaagaaaaga gaggaagtaa ataaaattct ggagtcggaa
     2041 aattctggtt acagattaag cgaaagcaat gaatttattc caatcacaga tgatatttct
     2101 attaagtcta taaatgctgt atctgaatca ccatttaatt acgcaaaaaa tcatatccat
     2161 aaatctctta catatataag cgagaaggaa aatcaagatt ataatactgt agtgagagaa
     2221 gcaattagtg ctgtcgaaag ttgtcttatc gaattttcag gattaccagc taataaaaaa
     2281 atactttggg aactgctata aaagcaatta aagacaaatc gaataacaat gtcgatctta
     2341 catttttaaa accatttgag acaatgtatg gtttggcgag taataatgga atacgacatg
     2401 cagaaaatga aaaaacaatt gtctcagatt tacctgatgc agttttggtg ttaacaacat
     2461 gttcagccgt aattaattat ttgggagtaa aaatagcata tgactccaat aaatcatgaa
     2521 aagttaaaat tcatccaaga gctacattat aactattaat ttccaactct ttaaaagttt
     2581 agtaattgaa ggaagtaata agggtgattg aacaatacgg atatacggtg tatacttatc
     2641 gtaggagttg atgatatgaa tattaaaaaa cgcaaaatca gagaagtagg taattcaata
     2701 gttgtaactt tgtcaaaaga gagtttattg caaaaaggtt tgaaacctgg agatactatt
     2761 tttattgatg aagataaaat gatggaagct attgtaaaag aggaaagcaa gatagatctt
     2821 gaaattgata tgtatgttaa ccaagcttta tctgagtatg atgcagcttt caaggaatta
     2881 gttgaaaaat gatctatcta agtgaaaaag atattgtaaa attgaatgtt attcaaataa
     2941 agaaatattc accgcgagaa acaatcggag tcattgacaa aaatgcgctg agtatggctg
     3001 taaatcaacc taaacagcat atttttggaa aagatctgta tcctgataaa tttagcaaag
     3061 cagctgttct gctaataaat ctgatcaaaa agcatccatt tcataatgct aacaaacgta
     3121 cagcattttt agccgcgtat gtttttctca agctaaatgg ctattcacta aaaatgaaaa
     3181 atcaggaagt agtagaattt gtagttgaaa ttgctacgta tcagggtgat tttgatgact
     3241 taaaagaggc aacaacagat atactaaaaa agaattcaac aaagaccgat tcaaaaaagt
     3301 agaagcaaac tagggattca gttaaatctg aagatttagt ttgttcttta ttttagttta
     3361 cataagatac attatgcgaa gttcattatt ttaaaagctt aaataataaa cttttttata
     3421 ataccaattt atttatgtaa accaatgacc ttaatcgctt aagcaagcat gttatgtaat
     3481 actctactct ctccaaaact aatgactatc tattttcaaa gaagctagaa tgattatctg
     3541 atcaaatgat aatttatatt taattcctgg tctactgctc ttcttttaat caattaatgt
     3601 ctaacgtact gtacacgata aagtaggaag acttattttc aacttcggat tcattttttt
     3661 aattcggtga aaatcactaa attaagagtt tatccaagtt cattttagct gagaatacgc
     3721 ttgaaaataa ttttgatgaa ttttcctgaa ttaaaattta ttaacttgtt gagtgatata
     3781 attgtaatgt attaccaaat agttagcact tagattagtg atggaaagga atagtgagaa
     3841 caagatgact ggtagaaatc ccaaagtatt tatatcatat tcatgggaga ataaggaaaa
     3901 taaagaatgg gctagagaac tagcagacaa attacttgaa aacggtattg atgcatatat
     3961 agatcagtat gatttaagtt taggtgatag attacctcag tttatggagc agcaaatatc
     4021 cgattcagat tatgttctaa ttatatgtac accagagtat aaaaaaaagg caaataatcg
     4081 tacaagtggt gttgggtatg aaggtcatat tatctcagca gaattgcttt ctggaaaaaa
     4141 tgaacgtaaa tttatcccaa taattaaaga aggacgtgca agcgaagtac ttcctacttt
     4201 tcttgctgga aagcttggaa ttgatttatc agatagccag cattatgatg aaaacttaga
     4261 tgatttaatt acaactatat ttggtgttaa gagaaaacca gcattaggtg atatacctag
     4321 gcatattaaa ggcagtcctt tactacctat agaacataat gaaaataaag atattgaaat
     4381 cttaggaatt atcactgata aagtaactgt acctaaaatg gatggctcaa ggggttctgc
     4441 attatataaa attccattta gattaaatag agtaccaagt cagctttgga aagagttgtt
     4501 tgttagaaat tggaattcac caccacgata tacaacaatg catagaccag gaattgcttc
     4561 tgttgtagac gatgaaattt tacttaatgg aacaactatt gaggaagtcc gggaatatca
     4621 tcgggaaact ttaaaacttt gcattgaaaa aagtaatact caagaaaaaa aaatattgct
     4681 agaggaggaa caaaaaaaag agagagagaa tgagcgagtc agatcacata aagaaagtgt
     4741 tcagaaatta gcagatgaat tagagttcta gttgatagtc agttctttct taattcgata
     4801 cacagtttgt ctagttacat tatagtctgt tgctattttt gaaatagcaa caccattctc
     4861 ctggtcctga acgatgcttc tataaacaag gcgacgttga ggatcttttg tgttcgcact
     4921 gtataactta ggacgtcctt tatagacgcc gtttgcttta gcaatcttta tcccttgtgc
     4981 ctggcgtcgt tttgattcag tacgctcttg ttcagcaatc atggccaaga tttgaatgat
     5041 taggtctttg ataaatttat ccaggagagg atttccgatt gcttcagcca tgatcggtaa
     5101 attagtgata ataagtcgca catttttctt ctttagataa tttactgaat cgataatctc
     5161 gtcataatta cgtccaagtc gatcgatagc ctctacaata aaaatatctt ggtctctaac
     5221 aaaatctaga acttcctgaa aaatcggtcg atgagtaata gtcgctccag attgtttctc
     5281 gatgaaaatt ttttcagcac caaattttct catttcctcg atttgacgtt gttcattttg
     5341 atcgatcgat
//
LOCUS       DACJGB010000130         5313 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.135, whole genome shotgun sequence.
ACCESSION   DACJGB010000130 DACJGB010000000
VERSION     DACJGB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.135"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="IYI34_002887"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="IYI34_002887"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAR0560700.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="IYI34_002888"
     CDS             complement(1298..2134)
                     /locus_tag="IYI34_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560701.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="IYI34_002889"
     CDS             complement(2318..3535)
                     /locus_tag="IYI34_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAR0560702.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3918..4001
                     /locus_tag="IYI34_002890"
     tRNA            3918..4001
                     /locus_tag="IYI34_002890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5238)
                     /locus_tag="IYI34_002891"
                     /pseudo
     CDS             complement(4241..5238)
                     /locus_tag="IYI34_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac ata
//
LOCUS       DACJGB010000131         5207 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.136, whole genome shotgun sequence.
ACCESSION   DACJGB010000131 DACJGB010000000
VERSION     DACJGB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.136"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            34..1368
                     /locus_tag="IYI34_002892"
     CDS             34..1368
                     /locus_tag="IYI34_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560703.1"
                     /translation="MDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAY
                     GLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVV
                     NLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITT
                     YTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQF
                     PWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQS
                     KHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAAD
                     NNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEIT
                     TKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1355..2989
                     /locus_tag="IYI34_002893"
     CDS             1355..2989
                     /locus_tag="IYI34_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560704.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            3164..3997
                     /gene="msrA"
                     /locus_tag="IYI34_002894"
     CDS             3164..3997
                     /gene="msrA"
                     /locus_tag="IYI34_002894"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR0560705.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQGERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            4077..5066
                     /locus_tag="IYI34_002895"
     CDS             4077..5066
                     /locus_tag="IYI34_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAR0560706.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT         1825 a          806 c          951 g         1625 t
ORIGIN      
        1 agtttttatt atggagaaaa gttttttaaa aagatagatg ctgctttttt atttagttgt
       61 ttatatactt ttgcaactta tcgaacagtt gatatctatt atcgatccgc aattgcagaa
      121 gcaatcgctc ttacgattat cccagctgtt ttatattatg cttatggatt gatattcaat
      181 agagaaaaaa aagtcatcgc tttatctgtc agtataagtc tacttgtgta tactcatgtc
      241 ttaactacac tattgtgtag tgcattgatt gcttgtatga ttatagttcg tttgttttta
      301 agcaagcaaa taaaaaacga tataaaattt gtggtcgtga atttagtaaa ggctgttttt
      361 ttgacttgtg cattaactag ctatttttgg tatcctatgt tgcagcagat gaattatcaa
      421 gaaatacaca gtcccttcaa aacagatttg cagctagaag cactttctat ttcagatagt
      481 atcattcaag cgttaaataa cgatattaca acatacacta tggggttatt aggattggtt
      541 gcgttgattt ttcctttatt catactgaaa gaaatggaga aaaaagaaaa aatcatctat
      601 gttggcgtag tttctacatg ggtatgcgta actaatttat ttccatggtt tttgtttcaa
      661 aaaacaccga taagcttgct ccagtttcct tggcgtatct taggtatcca aatctttttt
      721 tcttcaatga tcataacgct tgtttttatg aaaaagacta tgaataaaaa atattcatgg
      781 ttaatcattg gcgctactgt ggtgtttcta ctcgtcacgt ctatagcagc aaaagaaaat
      841 tacgcaaaag ttatccaatc aaaacacggt catattgtta tcaatgaaga aacactccca
      901 cgttatacaa catcagatat gggaggatta tacgattacg ccccaacaga agcgataaaa
      961 gaaaggcctc acttagaaaa acatgaagta aaagtaggag aatcgtggga aaaagggaag
     1021 tataaagctg cagataataa cattacttac acagtggcaa gcaacaaaaa gcaaaaagtg
     1081 acagtaccag tatatgctta tttaggtaca caggtaaaag taaacggtaa agtcgtaaat
     1141 gactcttata aaaaacatgg cttagtgaat gtggatgtaa atgctggaga aaataagata
     1201 gaaattacaa caaaatatac tccaactgct ctcatggctt tatggatcag tattctgact
     1261 tattgtttag tggtgtattc aagttttaaa cgatttgcac ctaataagtt gaaagattct
     1321 cgatatgtga ctaaaaagga gataaacaag aagaatgaaa ataaataaaa aagtagtaat
     1381 attgagtgga ttaattatag ttagtagtat atattctttc atctttttca aaaatacaca
     1441 gagtatttat accactggag atctttcttt tcacttatct cgaatcaagg ggttatcgac
     1501 tatttttacc agtccgatta attatgagac attcaattat actggatatg gtgtaaatta
     1561 tttttatccc tttctgactt tttttccagc tgtaatgtta tattggatga caaaaaattt
     1621 gattgtaagc tatattattt acgtttggtt attgaacttg tgtacaacac tcgtagctta
     1681 tcattatggc gagcggtttt taaaacaaaa aaaagcggca tttttattta gttgtctcta
     1741 tattttctct gcttatcgga cagtagacat ctactatcgt tccgcaattg ccgaagctat
     1801 cgcaataaca cttgttatcc ctgtattatt ttatgcttat caaatcatct ctggaaaaga
     1861 agaaaagtat ccgtctgtaa agctagcatt gagtatgagt ttgctagtgt acagccatgt
     1921 actatcaaca ttaatgagta cagcattaat tatcattttt atttttattc gtttggtttc
     1981 taaaggtttc aagaatgcag attttattgc tatatttaaa aaattattta gtgcggcagg
     2041 aatgacattg gttctgacga gtgctttttg gtatccgatg tttgaacaaa tgctctacca
     2101 aaaaatcaac aaacctagcg tgacaaattt atatgctcat gcatctaatg tgttcgatag
     2161 tctgactgaa gctatgaaca acgacttaac tacttactca atgggactag ttggtctgtt
     2221 atctttatgt attcccctga ttttatttaa aaaattaaca aacatagaaa aaaagattta
     2281 ttatggtacg tgtttgactt ggctagctac aactagtttg gtgccatggt atcttctgca
     2341 aaatacgcca gccaaattgt tacaatttcc ttggcggatc ttaagcttgc aaataatatt
     2401 ctcttctctg atattaacga tgatattttt caaaaataga agatacaata agacaagaga
     2461 actattttat ttatttatga ctgttttgtt attaatcact ttaactgttt ctagtaaaaa
     2521 aaatttcaat caaaaaataa tttctcagcc gggacatatt gttgtaaaca aagaaacagt
     2581 agaggcgtat accactaaag gaatggatta ttttttatat gattattctc ctatcgcttc
     2641 attggagaat aaggagcata taataaaaca cgaagcaaag attggcagtc aatgggtaaa
     2701 ccttccttat aaaacaactg gtagttctat catatataga gtaactaatg agaagagcca
     2761 agtaatcaca cttcctgtat atgcttattt aggaacgaat gtagaaataa atcatcaacc
     2821 tgtaaaggag caggtgaatg ctaatggatt ggtacaaata agcggagtca aaggagttgc
     2881 tgatattaaa gtaagcacaa gttatacagt aacgactatt atttcttatc ttgttacatt
     2941 aactgcatat ttatgtttat tttttaaaat agttttccat aaaaggtaaa ttaacttttc
     3001 atttataaaa taaattgatt aaaaaggaga atctgtcgat aaggattctc tcttttttat
     3061 acgaaaaatc tgtaggggga tgaagtaatc gcttatttcc tcactttatg ataaattaca
     3121 gctaaagtca ctagcttgtc ttcttcggaa aggaggagaa tctatggatg aggaacaaat
     3181 attcattcaa acactttata atctgatttt gaatccgaat acacgagatt gggaacgaaa
     3241 agtattgatt cagacaaaga atgacataag agaaaatata tccgttaaag aacagttatc
     3301 aaaactagaa gcaacactac gtccattagc tatccgaatg aatctgacgc ctgatgtaat
     3361 ggacttttat ttattgatta cggaaggatt tgataaggag caaaaatatg acttttccaa
     3421 gcatgccatg caagatgctg actatcaaga acgtgcagta tttgcagggg gatgtttctg
     3481 gtgtatggtc gagccgtttg aatcgaaaaa agggatccta tctgttttgt caggatacac
     3541 gggcggacat gttgaaaaac caaattatga tcaagtaagt ggtggttaca caggtcatgt
     3601 ggaagctgtg gagatcattt atgacacaag agaaataagt tatagcgagt tgctgacaat
     3661 ctattggcaa attactgatc ccacagatac atttggacaa tttcaagata gagggaagca
     3721 atatcgtcca gtgatttttt atcaaggcga acgacaaaaa gaattagcgg aacaatcaaa
     3781 acagaaacta gacagttcag gaactttcca ccaaccaatc gtcacaaaaa tcgaaccagc
     3841 cggtacgttt tggccagcag aaaactatca tcagcaattt tacaaaaaac aaccgaaacg
     3901 ttataaaaaa atccagcaag cacgtaatca atttctgatc tatcaacgag taaaaaacaa
     3961 atggcaaaaa aatatacgaa aaaatcattt tgattaactc cttttcttta aaatgtagta
     4021 aactaaaaat agaaaaaaga aagcgtttta aaaagatttg atgaggagga gaaaaattgt
     4081 atattgcaga tagtgcacgt tatgaaaaaa tggaatatcg taaatccggt cattcaggtt
     4141 tgaaattacc gattttatct ctcggattat ggcaaaattt tggtgattat gatcctatcc
     4201 ataatcaaag agagatttta agaggcgcat tcgacatggg aattacccat ttcgaccttg
     4261 cgaataatta tggaggccct gctggagctg cagagaaaaa ctttgggcgt atttttagag
     4321 aagattttca agcctaccgt gatgaactga ttatttctag caaagcaggt tatcatatgt
     4381 gggaagggcc atatggggaa tggggctctc gtaaatccat catttcaagt tgtgaccaaa
     4441 gtctccaaag aatggggcta gattatgttg acatcttcta tcatcatcgc ccagaccctg
     4501 atacaccttt ggaagaaaca gcagaagctt tgatgcaact tgtaagacaa ggaaaagcat
     4561 tatatattgg tatatccaac tataatggag aagatacaaa aaaaatgact gaaattttaa
     4621 aaagaaagga agcaccgttc attattcatc agatgcgcta caatatgttt tctcgtgcac
     4681 tactagaaga cgatctatct ccagtattag aagaagaagg gctaggggca atcacgttca
     4741 gtccgcttgc tcaagggttg ctgacaaatc gttatctaca tggtattcct gaagattcta
     4801 gagcacatcg taaggaaata ccattccttt cagaagaaca agttggatca actctggaaa
     4861 aaatcaaagc actgcaaaca atcgccgtat cacgaggaca atcattagcg caaatggccc
     4921 ttgcatggaa tttgcgacaa aaatctgtta caagtgtact ggtcggagca agtcggttaa
     4981 gtcagttaca agaaagtgtt cgaatgatgg ataatttaga tttctcacca gaagaagaat
     5041 tgagaatcga ccaaattcta gaatagaaac gatgtaatca aactttttat ccatatatta
     5101 aaaaaaacaa aagatgacat caagaatcga atgaagatcg tctgttattg aacatcatat
     5161 tttttgtcgt tcaaaataaa aatgatcgaa ggctaggagt gaatttt
//
LOCUS       DACJGB010000132         5061 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.137, whole genome shotgun sequence.
ACCESSION   DACJGB010000132 DACJGB010000000
VERSION     DACJGB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.137"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..476)
                     /locus_tag="IYI34_002896"
     CDS             complement(<1..476)
                     /locus_tag="IYI34_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAR0560707.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKEHKNVLRDIEKLKEDVLNF
                     EQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMEVLGG"
     gene            530..991
                     /locus_tag="IYI34_002897"
     CDS             530..991
                     /locus_tag="IYI34_002897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560708.1"
                     /translation="MKKLLYILLFSTFAIVALLLLFEHLKRVNLDGTLLEEQIAILNT
                     PKLLIVFFFMCMLSALIFMFYISTFWVYKEIKNKEFTDKDMIFMGFLVKILFIFSIVI
                     TLLFCLNETQFSVATTLISFIAIFSFIFSDNQKKSINGFLQKIKKKINGEP"
     gene            complement(1189..1440)
                     /locus_tag="IYI34_002898"
     CDS             complement(1189..1440)
                     /locus_tag="IYI34_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0560709.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(1454..1645)
                     /locus_tag="IYI34_002899"
     CDS             complement(1454..1645)
                     /locus_tag="IYI34_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560710.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            1938..2258
                     /locus_tag="IYI34_002900"
     CDS             1938..2258
                     /locus_tag="IYI34_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560711.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2276..2698
                     /locus_tag="IYI34_002901"
     CDS             2276..2698
                     /locus_tag="IYI34_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAR0560712.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            2781..3416
                     /locus_tag="IYI34_002902"
     CDS             2781..3416
                     /locus_tag="IYI34_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAR0560713.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            3534..4745
                     /locus_tag="IYI34_002903"
     CDS             3534..4745
                     /locus_tag="IYI34_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0560714.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         1789 a          837 c          833 g         1602 t
ORIGIN      
        1 cctcctaaaa cttccataac tttcatattt cctttttgct taatttcaaa ctcttgttgt
       61 ccgctaattc gcatagtatc tttaagcatg gagacatctt ttttaacatc tttcatttct
      121 tctagttggt agatcatcat atcttcaatt gtttgaggaa cagtattttt ccgaataaca
      181 tcttccattt cgttgaatgc ttcaatgtac ttctgtttga agtaaatagc tttctttcct
      241 gtaaatccca tagccaacaa gaaaaatcca tctctgctaa tgaagaaaac tcgccgattt
      301 ctaccgtatg aatctggttc attaccttcc acaaacatct gctcaaaatt gagcacatct
      361 tcttttaact tttcaatatc tctcaaaaca tttttgtgtt ctttttcaaa actatctgca
      421 acttgcaaac tcgtagtcac agcttcttta tttttcaaaa ttactaattc ttgcattttt
      481 tcttctctcc ttttgatata atttaggtaa aaaatggtgg tggaaatatt tgaaaaaatt
      541 gctatatatt ttactttttt caacctttgc aattgttgca ttattgttgc ttttcgaaca
      601 tcttaaaaga gtaaacttgg atggaacttt gttagaagag caaattgcga ttctaaatac
      661 tcccaaatta ctaattgtat tcttttttat gtgtatgtta tctgctctga tatttatgtt
      721 ctatatatca actttttggg tatacaagga gattaaaaat aaagagttta ctgataaaga
      781 tatgattttc atgggttttt tagtcaaaat tctttttata ttttccatag taatcacttt
      841 attattttgt ttaaatgaaa cacaattttc tgtagcgacc actttgattt catttattgc
      901 tattttttcc tttatatttt ccgataatca gaaaaaaagt attaacggat ttctgcaaaa
      961 aataaagaaa aaaatcaatg gagaacccta atataaaaat caggaaaaaa atattgatta
     1021 ctcctcttag gaagaccatt agtcagtccc tcccggctgg ctttttcgct ctgtactcag
     1081 cttcatcaag ccccataaaa atccaaacca tgtaaacgat cgttcctatc aacgcttgcc
     1141 tgcttcccca aagccctaaa gcgtagatga ttagcggtgc gctgaatact agtgctctgt
     1201 tgaatttgcc catgtttttc ctccttttag atactgatac aaagcgatac tactaaacct
     1261 ccactctctg ccaactttcg ctgctggaat ctttccagat tcggcatctt tagttagcgt
     1321 gcgtgtcgtg gttttcaaat attccgcagc ttgctttgta tcccacactt catttgcaat
     1381 ctcagattct gccaaagaag ctttgagggc tgagaggtta actaaagcta gttgttccat
     1441 tgattaattc ctcctatcga attctgtaat cacggatgat ttctaagata gttttattag
     1501 cttttggtcc actccaatgg ccatcaataa tttgctgcat tcgaacacgt gtgtatccat
     1561 atgcagtagc caaatcttcc atagttactc cgttttctcg cataaacttc ttgattgcag
     1621 cacggccgtt atctagattt gacatctatt cactcccctt acatatagat ttgtaagtca
     1681 aaatgataga aaaaacgtat aaactattga ctaatagtat acaatcgtat actatataaa
     1741 catagttaaa taagcctaca acaaaccctt tattatgcaa tcggtcgcca aacttaatgc
     1801 tataaggtgt atttttagtt tgcttttttt ctatcaaatt aacttacaaa caaataataa
     1861 tacaaactta tacttatgtc aacagtataa tttacatttt gtgtactttt atttgtttag
     1921 gattggagaa cattattatg acactgtttg aaaggataaa atcattagct agccaaagag
     1981 ataaaagcat gaaagaagtc gctttagaat taggatttag cgaaaatctt ttctatcgat
     2041 ggaaaacaac agaacccaaa gcaagagatt tgcaaaaggt agctgactat ttcaatgtct
     2101 ctgtagacta tcttctaggt agagaagaaa aagaaacgcc taaacatgtg gatttatcag
     2161 aagatgatac tgtattttct tttgatggaa aagaaatatc taaggagaca atgcgtaaag
     2221 cgattgcaat tgctaaagct ttagaggaaa atgaatagtt ggagtgatgg gttgtatgta
     2281 tttaaagttg aaagaaatgc tgagtgagta taatttaaag ttaatctata tggaaatgga
     2341 agaaccaggt ttttattatc caaaaccaag aatagtattt ttgaatgaaa aactacacga
     2401 agacagttct gaagcttttc atttagccca tgagctcggt catttcattg cttcacattt
     2461 tgaatattca gtactgtacg ataactctac aacttttcat tcaaaattcg aagctgaagc
     2521 tgatagaatc gcaattatga ttttactaaa tatctttatt gagaacgaat tgactgatga
     2581 atctcagttc aaattagaaa atttcatgga attctactct atcaataata agttaagaac
     2641 agaatgtttt aatgtttgcc agtcatattt caagaaaaaa tactcttatg cacagtaaaa
     2701 aaagcccgtg ctgcaacacg gactcatacc tcatttctga gatcacaaat atattataac
     2761 aagaagtgag ggatatttaa atggcaaaaa aagttatggg tcaagatggg aaaatgtata
     2821 aggttaaaaa accgttttat aaacgggtat ggttttgggt attaatagta cttttgtttt
     2881 ttggtattgg tggtgctcta agtggcggtg aaaacaaaga ttcagctact gcaacatcaa
     2941 caagtactaa taaagaaact acaaaagaaa ctacaacaaa agaagaaaag acttataaaa
     3001 tcggtgatga cgttgctgta gggaaaatgg aatacaaagt aaactccgtt gaagtagtaa
     3061 aacaagttgg cccatctgta cttcctacta acgcaaaaga cactttctta gtagttgact
     3121 tatctgtaaa aaatgcaggt gataaggctg tgactgttga tagctcattc ttcaaattaa
     3181 aagcagatgg aaaaactttc gaagctgatt cggctgcatc tatgtcggca aatcaagatg
     3241 aaaatggtaa tatcactaac tctttcttta tggaaagctt aaatcctgat atgcaacaaa
     3301 ctggtaaaat agtttttgat atctctgaag cccaagcaaa tgcacaaaat aatgtattgc
     3361 aagcacaaac aggttacttt ggaacagaga cagtttcaat tgctcttcat aactaattag
     3421 tataaaaaac acgccccacc gaccaaagcg agcgtgttct aagaaaaaac aaacctacac
     3481 aataggcttc tttatagtac ctattgtatc agagaaagag agctgattca attatgtcag
     3541 aagtaacagg atacttagaa caagtagaca atgaaacata tagactaaga gctgtattag
     3601 ggtacaaacc tgatggctcg gcaaaaagaa aaagcaaaac tataaaagcg aatagtaaaa
     3661 gagcggcata taaagaattg aacatctggt tagagcaatt cgaaggaatg accgatgatt
     3721 cgttggattt attcaatata acttttggtg aattttatag aaaaatatgg ctttcagagg
     3781 ccgaaaaaaa tttagagcca aagtcttatc ataactataa gcgaatgatc gagaatagat
     3841 ttcttgataa atttgatttt atccctctaa tcgatataaa accatacatg ataaagaaaa
     3901 ttgttgttaa tgctcaaaga atcaatacaa aagatcctgg aagaaactct gataaacctt
     3961 tatcaagaaa tacaaagctt cgtatgttat acgccgttaa caacttattt ttgatggcta
     4021 aaaatgagta cggagcaatt aaagaaaatc ctgtggaaaa tgtaaaaata ccaaaagaaa
     4081 aaggcgtaaa aaagaatatc gaagaacctt attcggaaga agaaattcat gcaatgctaa
     4141 aagcagcatt tgaggaaagc atagaaatta aaactttaat tgttctagca tttattacag
     4201 gagcaagaca aggagaaatt gcagctttag aagaaaaaga tattgacttt gacaaacaag
     4261 aaataagatt tcatcagcgg atttcagaag tggatggaaa atcagatatt cggttgctgc
     4321 caggtttgaa aaatgatgat gatgaaaaaa tagtaaccgg tcctgcttat ctatttgata
     4381 tgttagatga attgatcaaa gagaataaaa aaattcgttg gaaattaaat ataaagaaat
     4441 taagacacta ttttatattt gatacaaaac aagacggaac tttaccacgt ggaagttatc
     4501 tgtataagaa gttcaaacga ttcaccaaac gtcacaattt gcgtcacatt cgttttcacg
     4561 acattagaca tacatcagct acctatttgc ttagcgatcc taatatgact ccaaaagagc
     4621 ttcaaaaacg attaggacac cgagatttta atactacaat gaatgtttat ggccatgtct
     4681 tacgcaaaga aaaagataca gcaactacag cgtttgaaaa actattaaaa aaagatataa
     4741 aataacacta aaaaattata ttttcgggta taaactcggg taggatatat aaaaacttgg
     4801 gataatttgc ggtatataag catttaaaaa tgctgtaaat tcgcttatct tcatttaaaa
     4861 taccattaaa taacgaactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
     4921 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
     4981 gctgttcttt ttttattttc gccgtttgta taattaagcg agacaaataa aaaagccatt
     5041 tatgttacac tcaaattgta a
//
LOCUS       DACJGB010000133         5024 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.138, whole genome shotgun sequence.
ACCESSION   DACJGB010000133 DACJGB010000000
VERSION     DACJGB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.138"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            282..521
                     /locus_tag="IYI34_002904"
     CDS             282..521
                     /locus_tag="IYI34_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560715.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="IYI34_002905"
     CDS             complement(883..1143)
                     /locus_tag="IYI34_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560716.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="IYI34_002906"
     CDS             complement(1328..1825)
                     /locus_tag="IYI34_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAR0560717.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IYI34_002907"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="IYI34_002907"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAR0560718.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IYI34_002908"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="IYI34_002908"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAR0560719.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..>5024)
                     /locus_tag="IYI34_002909"
     CDS             complement(4547..>5024)
                     /locus_tag="IYI34_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAR0560720.1"
                     /translation="CIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIV
                     SVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAF
                     YYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1042 c          771 g         1682 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaa
//
LOCUS       DACJGB010000134         4947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.139, whole genome shotgun sequence.
ACCESSION   DACJGB010000134 DACJGB010000000
VERSION     DACJGB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.139"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            128..1771
                     /locus_tag="IYI34_002910"
     CDS             128..1771
                     /locus_tag="IYI34_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAR0560721.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(1844..2389)
                     /locus_tag="IYI34_002911"
     CDS             complement(1844..2389)
                     /locus_tag="IYI34_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAR0560722.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(2412..2897)
                     /locus_tag="IYI34_002912"
     CDS             complement(2412..2897)
                     /locus_tag="IYI34_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAR0560723.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(2887..3699)
                     /locus_tag="IYI34_002913"
     CDS             complement(2887..3699)
                     /locus_tag="IYI34_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAR0560724.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(3838..>4947)
                     /locus_tag="IYI34_002914"
     CDS             complement(3838..>4947)
                     /locus_tag="IYI34_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAR0560725.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT         1593 a          977 c          946 g         1431 t
ORIGIN      
        1 aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt ctagttaccg
       61 gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt taaaggagtg
      121 aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc aaatctatcc
      181 tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga tccgtgaaaa
      241 acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga tctatcaatc
      301 tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag agagattcgg
      361 aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga tcaaagtgat
      421 tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag aatcgaaaaa
      481 atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag atggcaaaga
      541 accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc atgaaactgg
      601 acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg aaagcgagaa
      661 attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga tagatggatt
      721 tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag ccacagagaa
      781 ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg atttgaaaca
      841 tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca ctgcagaaga
      901 aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat ttgatcatat
      961 ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga aacttgcatt
     1021 aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata tggaaaacca
     1081 tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt gggctatgtc
     1141 tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta tttatcaagg
     1201 acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg atgcagtaga
     1261 ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag aagcattgga
     1321 tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga cttcggaaca
     1381 atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata ccaaaacgat
     1441 caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata agaacatgat
     1501 ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat atcttcatga
     1561 acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc ttattatggt
     1621 aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc agtcctggac
     1681 attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac tagcacctta
     1741 tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc attagttgga
     1801 ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa ttcgctctag
     1861 gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt ccaagggttt
     1921 tcaaacgagg tctgacaggt acttggacat gtttgatatg cataccgatt gatgtgtcac
     1981 cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca ttgaactggt
     2041 caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca gagacaattt
     2101 cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc aaatgttcac
     2161 aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac gtacggatga
     2221 tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg acttcgcttg
     2281 tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc aatatgtcat
     2341 taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt ttctaaaacc
     2401 ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc agttatagga
     2461 aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga agccactcca
     2521 gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat aactaagctt
     2581 cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga accagcaaca
     2641 tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata tcctgaaatc
     2701 aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg accataaaga
     2761 aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt agccggtaca
     2821 gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc atagatcgtt
     2881 aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc agacacttcc
     2941 ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt ttttactttc
     3001 tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg cagaagaaaa
     3061 ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa atacctcata
     3121 atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg tgccgccttt
     3181 gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga tcatttctgt
     3241 taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat taggcgtaat
     3301 catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt gattgatcac
     3361 agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat tttgttttgt
     3421 ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc caattttgat
     3481 accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca attccaaaga
     3541 tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag tcaaggcgct
     3601 tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc ctccaccagc
     3661 aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct ccatgaataa
     3721 gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt atctcatcca
     3781 atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct taaatagtta
     3841 ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca ttgtagctgt
     3901 tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa gttcatggac
     3961 ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa taatcggttg
     4021 tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta acgaatctaa
     4081 taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca cttgccgatc
     4141 tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat cccaccaatc
     4201 gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag cccgtccttt
     4261 agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa ctgaatctgt
     4321 aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat cacttgtttt
     4381 caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt ggccatatac
     4441 ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca ttgtctggtt
     4501 gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt ctggatgctc
     4561 cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg caattggccg
     4621 gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag gcatcatagg
     4681 aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca ctggagagat
     4741 gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt gccatttcgt
     4801 ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag ttgtaccaga
     4861 agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa cagacaaagc
     4921 aggttgttcc attaaataaa gaatagg
//
LOCUS       DACJGB010000135         4924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.140, whole genome shotgun sequence.
ACCESSION   DACJGB010000135 DACJGB010000000
VERSION     DACJGB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.140"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            83..970
                     /locus_tag="IYI34_002915"
     CDS             83..970
                     /locus_tag="IYI34_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAR0560726.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1110..2036
                     /gene="cysK"
                     /locus_tag="IYI34_002916"
     CDS             1110..2036
                     /gene="cysK"
                     /locus_tag="IYI34_002916"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAR0560727.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2272..2718
                     /locus_tag="IYI34_002917"
     CDS             2272..2718
                     /locus_tag="IYI34_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAR0560728.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2991..4343
                     /locus_tag="IYI34_002918"
     CDS             2991..4343
                     /locus_tag="IYI34_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAR0560729.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4514..4801
                     /locus_tag="IYI34_002919"
     CDS             4514..4801
                     /locus_tag="IYI34_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560730.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1693 a          813 c         1030 g         1388 t
ORIGIN      
        1 ttaaaagtat aatttaatta attaagtttg ttttaagaag agaacttttt attaagtttt
       61 tttattttga ggaggaattt gaatgcaaga gctgaagacc cgcagtgccc ggcaccatag
      121 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
      181 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      241 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      301 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      361 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      421 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      481 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      541 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      601 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      661 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      721 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      781 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      841 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      901 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      961 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
     1021 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
     1081 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1141 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1201 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1261 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1321 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1381 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1441 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1501 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1561 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1621 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1681 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1741 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1801 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1861 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1921 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1981 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     2041 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     2101 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2161 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2221 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2281 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2341 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2401 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2461 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2521 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2581 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2641 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2701 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2761 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2821 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2881 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2941 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     3001 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     3061 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3121 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3181 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3241 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3301 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3361 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3421 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3481 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3541 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3601 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3661 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3721 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3781 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3841 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3901 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3961 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     4021 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     4081 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4141 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4201 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4261 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4321 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4381 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4441 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4501 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4561 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4621 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4681 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4741 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4801 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4861 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4921 cccc
//
LOCUS       DACJGB010000136         4734 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.142, whole genome shotgun sequence.
ACCESSION   DACJGB010000136 DACJGB010000000
VERSION     DACJGB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.142"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(16..1101)
                     /gene="xerS"
                     /locus_tag="IYI34_002920"
     CDS             complement(16..1101)
                     /gene="xerS"
                     /locus_tag="IYI34_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAR0560731.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3177..3695)
                     /locus_tag="IYI34_002921"
     CDS             complement(3177..3695)
                     /locus_tag="IYI34_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAR0560732.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1555 a          777 c          717 g         1685 t
ORIGIN      
        1 tattttttac tacatttaca aagcgttcaa agcatcaatt gtagctgcat tgtctatatg
       61 agtatataaa gttgttgtat ttgtacttgt atgtcccaat tgttgggcag ttaataccaa
      121 tgaatttgtc tgttgataga gtctagtagc caaagtatga cgtaatttat gtggactgac
      181 ttggactctg aatccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
      241 acgcttggct tctccttgat aaattgctag aaataaagct ttttcgtttt ctgttacttt
      301 gtatcgttct ttccgaatag ctagataatt agctaaatat tccattgcaa atgctgctat
      361 atttactgcg tctctcttat ttccttttct agttactacc acattattct tttctaaaga
      421 taggtcctct aaattaatat ttacaacctc ggagagtctt aatccagatc ctaaaatcaa
      481 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttgct
      541 caaagttttt ttatattcat tggcaatata atctagataa ttttgtgtat cttcgtctag
      601 aaataatttt ttctcgattg cgtttgcacg ggcagctaac gtagttttat ctgtgatagt
      661 tgctacttga tccataggat tattttctaa gtatggtttt cctaaaggat tattggaact
      721 tttcgaaaga tatttaaata gaacctttaa tgctgtgata tttctattga tagtagaagt
      781 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
      841 ttcctttgtg cgacgtttca aatgagattt gtacaagttt agatcatagg cagttaactg
      901 tcttatcgag gtgattgaag tatcgggaac gatagactca ctcagaagcc attcataaaa
      961 gagcttataa ttttttagat attctaaagt agttcgaggt gattgatctt tatcctcacg
     1021 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
     1081 atatatttct gtatttttca tgttattcat cctttcgata agtaggggag aggagtatct
     1141 ctataattgg actataccaa ttatagagtg ttttgtcaat tggtatactc tgaaatttat
     1201 taatttattt caaaaaagca gataaatagg tatagattta ataatttaaa ataaatggta
     1261 tacatgaata ggaaaggaaa cagaaggagg caatcttaat tttttaaatt gtctaaatta
     1321 tatagaaaga acaatctttc tatataatta gacataagag agactacggt tgatttgata
     1381 ttaacaagcg tatatgaatg tatatacaaa taaaaaaggg gagggagaag ttaaagaaaa
     1441 ttcagagaag tggggcactc agtagtatta acaatttaaa aggaatttct taaagaaaat
     1501 gggttgcatc ctggagatac aattttttta gataaagaaa aaattcaagc aacaattcaa
     1561 aaaaatctta actgctgaga ttgatgtagc aatatctcaa tcatcaacaa agtacgatcc
     1621 tgcctttcaa aagcttattg ataaatgttt attcattata aaaataaaag aaaaatttat
     1681 gtcagctttt ttcatcaaag tggtaatgat aattcgtcta ttaattgtat tttttaggaa
     1741 atatggtata attgttttaa cgaaaaggta attcatgata caccaaaaac cactagctgg
     1801 cgggctagtg gttttctttt acgttctatt cagattaacg ggtaggctta ctgtcttttt
     1861 tggcaataat tttgcaatca agccatttac agatgtaatg cgcggatact tctgctaaga
     1921 ttgcaaaaaa catatcgaaa aggtaattca taatacaacc tcctcccctt gccataatag
     1981 gccggggtag cagacagcat aaatattata ccataactga aaatttcatt ttagtacatt
     2041 tcaatatatt gatgaaccag cacaataaaa catgccatga ctaacagaaa aaaacttatt
     2101 aaaccagata taacttgtaa acatccaact atatagaaaa cgtaaatttt ctatatagtt
     2161 ggattaagat aacgactatt tattttatca tatttttact ataccctata cttttacttg
     2221 gtatatttat gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat
     2281 aaaaatacta aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt
     2341 atttagataa cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct
     2401 actcaatcga aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag
     2461 accttttaca tcttgcagaa atcgttactg ggattaaact aagcaataaa aactaatgtt
     2521 cccctcattc tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac
     2581 cttcaggatc ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag
     2641 gataggcgat aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg
     2701 tatgttggct ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa
     2761 tcgttcttaa caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt
     2821 gccttaccct tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc
     2881 ggcggtctga aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc
     2941 ctttgtagcg gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt
     3001 tggccaacca aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc
     3061 acttgcattc acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct
     3121 tactgttaac atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac
     3181 aaagctaatc tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta
     3241 tttacatgaa taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat
     3301 tttcttttgt gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata
     3361 tcatcaatat tacgattgag aatttcctct aattctttca atggtagatg tgcataggta
     3421 tttgtaaacc acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt
     3481 ttaacagata atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca
     3541 acttgatatg ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc
     3601 aattcttcag gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct
     3661 ttttttaatt cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat
     3721 tgtctccttt aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat
     3781 atctgatatg cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc
     3841 tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg
     3901 tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact
     3961 actatcaatt actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa
     4021 gtgaggatag ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc
     4081 tttatatagg attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg
     4141 aagtaaaaat tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac
     4201 gaaatgatca actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg
     4261 ctcgacgaac tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag
     4321 ctttttatgt tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc
     4381 tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta
     4441 aaattatgaa taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct
     4501 ggtgttatag attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa
     4561 accgatactc tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa
     4621 acttcactaa cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt
     4681 atcaaaaatg ctgctcattt caacttatat gttttagtga actaccccca ctta
//
LOCUS       DACJGB010000137         4587 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.143, whole genome shotgun sequence.
ACCESSION   DACJGB010000137 DACJGB010000000
VERSION     DACJGB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.143"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            186..2855
                     /locus_tag="IYI34_002922"
     CDS             186..2855
                     /locus_tag="IYI34_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAR0560733.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="IYI34_002923"
     CDS             complement(2946..3566)
                     /locus_tag="IYI34_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0560734.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="IYI34_002924"
     CDS             3680..4219
                     /locus_tag="IYI34_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560735.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="IYI34_002925"
     CDS             4216..4554
                     /locus_tag="IYI34_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAR0560736.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DACJGB010000138         4548 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.144, whole genome shotgun sequence.
ACCESSION   DACJGB010000138 DACJGB010000000
VERSION     DACJGB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.144"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            82..1031
                     /locus_tag="IYI34_002926"
                     /pseudo
     CDS             82..1031
                     /locus_tag="IYI34_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(1076..1709)
                     /locus_tag="IYI34_002927"
                     /pseudo
     CDS             complement(1076..1709)
                     /locus_tag="IYI34_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            complement(1969..2517)
                     /locus_tag="IYI34_002928"
     CDS             complement(1969..2517)
                     /locus_tag="IYI34_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560737.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2518..3375)
                     /locus_tag="IYI34_002929"
     CDS             complement(2518..3375)
                     /locus_tag="IYI34_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR0560738.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYVEVE"
     gene            complement(3661..3948)
                     /locus_tag="IYI34_002930"
     CDS             complement(3661..3948)
                     /locus_tag="IYI34_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAR0560739.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(3938..4267)
                     /locus_tag="IYI34_002931"
     CDS             complement(3938..4267)
                     /locus_tag="IYI34_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAR0560740.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(4344..4490)
                     /locus_tag="IYI34_002932"
                     /pseudo
     CDS             complement(4344..4490)
                     /locus_tag="IYI34_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
BASE COUNT         1379 a          877 c          658 g         1634 t
ORIGIN      
        1 aagaggacag catatcgata ttgcttcctt ttttttgcta tttatttaag ggatagaagt
       61 ttagaaagga ctgtttataa gatgaaaaat gatatagtga cttatgcaaa cacaaaatgt
      121 gaagaaattg gcatcagtaa tttagaagag tgcttttttg acacaaatga cctgtcgcaa
      181 tttttaagaa cgggaagaac aaatgccagt aaacttgcca atgacttagt tgcaaagaac
      241 gttctattta aaatcaatac tcgtccagtg ttatttgcca tcaaagagcg ctttgaagag
      301 cactttttga ttccggtaaa agatgtatac gaaagtatat ctgaattaaa aacagacttg
      361 gagcccaatt ctacagattc cattctacaa aagatgattg ggtatgatat gagcttatcc
      421 gatgcggtgg aacaaatgaa aactgctgtc ttctatcctg gaagagggct tcctatgatg
      481 atcactggag atactggagt tggaaaaagt ttttttgttc atactgctta tcagtttatg
      541 aaaaaagtga acgttattca tgagaatgct ccttttaaag tacttaattg tgcgcaatac
      601 cataataatc ctgaattatt gtcggggata ctatttggct atgttaaagg tgcttttacc
      661 ggcgcagatg aagatcgaaa tggattgttg caagatgcag acggtggagt actgttttta
      721 gacgaagtac acagattgcc tcctgaaggg caagagaaac tatttgttta tatggatggc
      781 ggaacttatt ctagagtcgg tgaaactatc catcacgcct ccaatgtacg tttagttttt
      841 gcgactacag aaggcaaaga ggattttctg gaaaccttta tgcgaagaat ccctatacat
      901 atccatatcc caaatattga ggaacgaggt attttagaaa aaaacaaatt atcgaaaata
      961 ctctctttag cgaaagcaaa aactttagca aggctattga tgtttctcct aaagtcacta
     1021 gtgttgcata aatatagtca gaatattctg aattattatt ttcttgaaaa aagtaaaaaa
     1081 aatcgtatcc caaactttca tctacatttc cagaaagatc ttcatgttga taatcaattc
     1141 ctgaatcaat gaccgctacc ttcacatcct tagaaccctt ggtttcttcc caagcggatg
     1201 gcatgctaat ctctcttaaa ttagcttgtt tactccaatt aggatcgtta tcgatttcct
     1261 aaagaatcag ttgtatttgg ctcctcaaca tcattcaacg gctctagttc aaccacatag
     1321 tttggctctg cataattgac catatcagaa gcattaaatg ttcttactgc ctcttccaca
     1381 ctaatttctt cgttcaattt cactaataat acttgatctc gcttattata atcgctttca
     1441 tttgtcctaa gagtcaaatc tttttccacc ataactttta cacccaataa attttgcggt
     1501 ttatttttct ctgttgaaat tctttgtgta gcctttaaac tgacaattag ctcattgggt
     1561 acatactcta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
     1621 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
     1681 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
     1741 gttagctcta ttacttattt actaactcaa caacagatta tcatacactt attaatgaaa
     1801 tcacaatatc tcattaaaac acaccctaaa atcataaccg ttcctatgct cattttttgg
     1861 attttttaat cacgtaacca gaaaaataaa tcccctctat ttcacgcaaa tcaaagaaga
     1921 actaagtaac taccgtttaa aggtagttac ttagttcttc tttgattatt atctaacttt
     1981 attattcatt ccccaccctt tgcgcagtga gtcttcggta tcttgtttta tctgactggt
     2041 agttcttatt atttctccac gatcatctaa ttctggaaca attggatatt tttctctgaa
     2101 caagtctagt tctttcacca cgtcattaaa caaatcttgt ttatcttctt gcgtaagatt
     2161 ctgtttaata tactcatcta aagcttttct aagcaattca tctcttgtca tattagtctc
     2221 atacttttct actaaaactc tggaagtcgc atttaacaaa gtcgacagtt ttaaaggatg
     2281 cttggtggaa taaaccttat caaactttct gattggtgcg ccaacttttt ttgttcttcc
     2341 tctactctgt ctttcaacat tggaggaagt ttttatttct tgaacttggt gcacttctaa
     2401 ttgcttattt tcagatggtg taattgcttg ctgctttttc tttgcttctg cttctttttt
     2461 tattttggcg agtcctttgt tgttcccaaa aatatcaagt cctttgatgt cagccaatta
     2521 ctccacctcc acataccaat tctcgtgttc ttccaattct ttcaaaatac tgataaataa
     2581 ttcgtggctt ttgcgatccc attgttttaa gagacctttc tttgtcttct ccatcgtaat
     2641 acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt acaacatttt taatcaaatg
     2701 ttccccataa atctcttggg cttgtttata catttcctga tctactgcgt caccgctttc
     2761 caacatgaaa ggaataacac cagcaacata taaatttgta tcaaattctt tcacaaaaaa
     2821 gtccatatac tcaagatatt gcatcgcatt tctcatcgat tttacttggg tctgtaagac
     2881 gacgattgcc caatccgcaa tgtacatcgc ggatttacta tatacagaaa tagaaggtgg
     2941 gacatctaaa tagatgacgt caaactcatc aaataatggt tctagctttt ctttcatata
     3001 tttgaattgg gataatgaat cattatattg gtctttgatc caatcatcat acatttcaaa
     3061 ggacatgtct gctggaagta tatagaggtt attgattact ttatagacag cctctttagg
     3121 tggaagattg tttatcaaca cgtcagtcac agtattttcg taaacaatcg gttcaccttc
     3181 tccgcctcct tcttcgttag tgaagttcat atgcgttaag ttcatcacat cagaagtatt
     3241 tccttgcata tccaagtcaa ttatcaaggt tctttttcct tcattaactg ctgattcaaa
     3301 ccctaacatt tgcgcgcacg tacttttacc tacgccacct ttaaaattac cgattacata
     3361 tttcttcgct accacgttcc tacctctttt cttttagcaa tattcatttg atactattat
     3421 aacatattta tttttagtta ctaccaaaaa aaagatatat atgtactacc ttataattaa
     3481 taagtaaaag gtagtacata aataaggaaa agctgaaata tattgattta tagctttcct
     3541 caaagtaatg acgttattta atttatcgag aagcagagaa tactcgcgac tttagtcatg
     3601 tagacgaatc agattttcga taaaaaagaa gccgtccttc ttaagaacga caacaaatca
     3661 ctagattaat tttttcaact gttcataaaa gttttcatgc gttccacata acaatactgg
     3721 aataaccttg ttttctataa tctcgtaagc tactctatat gttgtgcccg catatttgaa
     3781 cccactagac caaatacctg ataaatcccc agttttttgt tcacccttat atggatcaat
     3841 cgcaatttca tcataaatta aattcaaaaa tttttctttc aatggcttct cttttagctt
     3901 ttttaaatac ttaatgacat gttttgaagg aacgatttca taagccaatt tctgttgcca
     3961 actcctcttt agtcattact ttagaattag taagtgtatc ttcagcaata tctctaaagg
     4021 aagaatgcat tttgtttttt ctattcttaa attctacaag aatctcatct ttatcgtagc
     4081 cttctgcaat tatatcagac aaaatatctt cgctaaaatc ataaaattct ttttgtggtt
     4141 caacaaattc ataaagaata ccattctcta ccaactttgc ttcaacttcg actccattag
     4201 gaacattaaa atctttaggt actgtcaaca tgattgaatt gccttgtttt cttgtttttg
     4261 taatcattta aatccctcat ttctaacagt atgtaattac ttagtaaata cattatacca
     4321 cgagataatg tatttacaag taattactgt gactcgtaat gcgtccttgc tgaataaatt
     4381 tcaaccacgt gttcatcttc atctacctta taaacaaccc ggtgttgtct attcaatctt
     4441 ctaaaatacc taccttcatg tgtaggtttt aacttctcaa atgattgggt tctgttgcaa
     4501 agttttaaat aaagaataaa atcccttacg gtatctatga tttaagct
//
LOCUS       DACJGB010000139         4477 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.145, whole genome shotgun sequence.
ACCESSION   DACJGB010000139 DACJGB010000000
VERSION     DACJGB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.145"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(832..1125)
                     /locus_tag="IYI34_002933"
     CDS             complement(832..1125)
                     /locus_tag="IYI34_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAR0560741.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1528..2706)
                     /locus_tag="IYI34_002934"
     CDS             complement(1528..2706)
                     /locus_tag="IYI34_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0560742.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="IYI34_002935"
     CDS             complement(2810..3124)
                     /locus_tag="IYI34_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0560743.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="IYI34_002936"
     CDS             3231..3446
                     /locus_tag="IYI34_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560744.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="IYI34_002937"
     CDS             complement(3746..3988)
                     /locus_tag="IYI34_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0560745.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="IYI34_002938"
     CDS             complement(3975..4403)
                     /locus_tag="IYI34_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAR0560746.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DACJGB010000140         4444 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.146, whole genome shotgun sequence.
ACCESSION   DACJGB010000140 DACJGB010000000
VERSION     DACJGB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.146"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(459..884)
                     /locus_tag="IYI34_002939"
     CDS             complement(459..884)
                     /locus_tag="IYI34_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560747.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1258..2229
                     /locus_tag="IYI34_002940"
     CDS             1258..2229
                     /locus_tag="IYI34_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAR0560748.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2261..3658
                     /locus_tag="IYI34_002941"
     CDS             2261..3658
                     /locus_tag="IYI34_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAR0560749.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            3817..4440
                     /locus_tag="IYI34_002942"
     CDS             3817..4440
                     /locus_tag="IYI34_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560750.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
BASE COUNT         1556 a          714 c          904 g         1270 t
ORIGIN      
        1 aattactacg tgatgtagaa taagcctttc tatcgtttgg ttattttata ttcaaatatt
       61 tgaaataaat aaatctttac tctttgattt gtaaactgaa atgatccact gtttgtttta
      121 ctgatgaagc ttgatcaaag gaagaagctg agtttgaagt tcataaaaat cagccatagt
      181 gagttttgct tggatttgtt tatcttcttc tactttttac ataattttga acaggataag
      241 attcaaatgt tttaaatgaa tctttgacta gtttgtgaca gttgtcgcat atgctcaagc
      301 ttccttggtc attaaattct gtatggatat ttttgttgtt attacatgta caataatcag
      361 gactatcgat catccatttc gtctccttta caatcatctt gcttctttac tatagcagaa
      421 aagaagggga gaaacaaaag aagatccaac agacggcact atagactgta gatgttgcca
      481 tcctcatcct tcaaacggta attaccgatt tttgtaaata tgactgttgt ttctttttta
      541 ttttcttttc ctgaaataat atattcgtca tctttggtag tgatcttgat atctgtcagc
      601 actgtttttt cttcagatga ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc
      661 cattccattt ttttatctgt aaatgtcaac tgtcccgcgg ataaatccat atctttacgt
      721 acttttagcg tatatacact gttggtggta tctgcttttt ccgcttttaa gacagaagaa
      781 tcggttgttt cgtccgagga ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc
      841 aaaagtcccg cagacaaaat aaatagtacg attaatttct tcaaaatact tcccccattt
      901 gataggataa agaaattata ccattggaat gtaaaacttc aaacggatgg aagagaaaaa
      961 cgcacaaaac acccacaaat agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat
     1021 ccgccacttt cagaaagcgt tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt
     1081 aaaaatgaac cggttcaaat acttgtttaa gtataattaa tagtgagagg aagttgctct
     1141 actgaagact ttagttatat ggatattgat gacaagcaac actatgcaaa gaatgcggtc
     1201 atcataatcg aacaacaaga aagagaaatc aaatgtaact ggcaggagtg agaatgtatg
     1261 gcatcgatcc gtgatgtggc taagatggct ggtgtttcag tgggaacagt ttcccgttac
     1321 ttgaatggtc agcaactgaa ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg
     1381 ttggactata aagaaaacat cattgctaaa gggttgaaga ataatcgaag cttctctatt
     1441 ggacttttga taaacgggat ttccagccgt tttgggtcgg aagttgtctc agggatcgaa
     1501 agagtagctg aagaaaatgg gtatagctta ttattaagcg ggtttgccga tcagccagaa
     1561 aaaattgaac aaaaaattga atacctgatg aagcatgtga tcgatgggct gattgtcttc
     1621 ttatccgaag aagagtggtc tggatttgaa atgctcacaa aaatgagtat tccagttatc
     1681 gcattgaatt gtccagaagg accagcaggt attgatacaa tattagtgaa tgatcgagaa
     1741 agtgtagcaa aagtgatcgc tcatcatggt gaacaaggac ataaaaaaat cggatttatt
     1801 gctgccactc aaacagacta cgtagcaaga gaacgtttag caggagccaa agaagcggtc
     1861 caaaatgatc catcgatcca attagaggtc tttacaggag actattctag aaaaagcggc
     1921 tattacgggg caaaagcatt attagaaaaa ggggcaactg ctatttttgt cagcaattac
     1981 aatatgtcga ttggtgccat cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat
     2041 attgcgttca gtcactatga ttatttagat aaaaacgatc tgagtatcct tccgaaaatc
     2101 acgatcacgc caccaacaga taaaatcggg gaacgagcag cagaacagct gttaaaacga
     2161 atcaaaaaag aagaggaaga aaatgaaaaa gtgattgtga tggaaaatat catacatgga
     2221 atcaactaaa acgaactaaa aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc
     2281 agcaaatttt tggtggggtt ctgcagcaag tggcccacaa acagaaggaa ggatttcagg
     2341 cgatggaaaa ggagagaaca tttttgatta ttggtacaaa aaagaaccgc aaaagttctt
     2401 tgatcaagta ggacctgaaa aaacctcgca agtttatacg aagtatcaag aggatgtaca
     2461 gttgatgaaa cagactgggc ataattcatt tcgaacatct atccaatgga gccgtttgat
     2521 acctgatgaa tctggaacaa tcaacgaaga agccgttcgt ttctatcatc gttattttga
     2581 tgagttgatt gaaaacggta tcgaaccctt tgtgaatctg tatcatttcg atatgccgat
     2641 gtatctgcaa gaaaaaggcg gatggttgaa ccgagaaacg gttgaagcct atgaaaaata
     2701 tgccaaaagt tgttttgaat tgtttggttc gaaagtaaaa aaatggttta ctcataatga
     2761 accaatcgtt ccagtcgaag caggttattt atatcgatgg cattatccag aagagtcaga
     2821 tatgaaaaaa gcgatccaag ttggctatca tgaagcgttg gcttcagcgt tagccatcaa
     2881 atcttaccat gaaatggagc aaggtggtgt tattggtatc atcttgaatc tgacaccaag
     2941 ctatccaaga gacgaatcaa accctgaaga tgttaaagca gcgcaaatag cagatgcgtt
     3001 cttcaatcgt tcattccttg atccagctgt aaaaggcgag tttccaagtg aattgattga
     3061 gatagtaaaa gaacttgatc tcatgcctgt gatccatgca gaggatttga cggttatcaa
     3121 agaaaataca gtagatcttt taggtatcaa ttattaccag ccacgccggg taaaagcaaa
     3181 agaaacacca atcgatacta aaaacggtcc aatgcctgag gattatttcg acaattatga
     3241 aatgcctggt cgtaaaatga atccttatcg cggatgggag atctatgaac aaggaatcta
     3301 tgatattttg atcaatgtac gagatcatta tggaaatatc gactgcttta tttctgaaaa
     3361 cggtatgggt gtagaaaacg aagcacgatt tatcaaagaa gatggcatga tccacgatga
     3421 ttatcggatc gaatttgtcc aatctcatct gaaatatgtc catcaagcca ttcaagaagg
     3481 tgccaattgt ttaggctatc atatgtggac atgtatggat aactggtcgt ggaccaatgc
     3541 atataagaat cgatatggct ttatttcagt agacttagca caagatggaa aaagaacaat
     3601 caaaaaatcg ggtcattggt tcaaagacgt atcagataaa aacggatttg aggcttaaga
     3661 aagaatagtt gagagcaaat gcgtgaaatt ctccattctt accattttgt gaaaaagaag
     3721 ttcaattatt tacgatcttt ttcaactttt ttgtctttct atttcatttt ttgtatataa
     3781 tagaatagtc aaaattaaat aggagtggag gaaagcatga ggaaaaaaag accgtttctt
     3841 ctcttttggt tattcggttt tttcttactg cttattttat ccgcagcatt ttcaggaaaa
     3901 atgacagcag atcttgctca gaaagaaaca gaaaaaatcg tagcaagcaa agaatcagta
     3961 gataatttca aaaaagaatt ctcaacttat tggtgggcgg agtataaaat aactgccaat
     4021 tacgaaaaag gcgaattgga catttatttg ccaaaaggaa aagaagaaaa tcaatttatt
     4081 aatatcgatg acgtacttag tgcgatttct ctcagacaat atcaatttaa agacaagcat
     4141 ttgaccgttt tgcttttgac tcatgatggc gacaaagtag cccgtttgaa aaatggaaaa
     4201 gtgatcaccg cttattctga aaaagtagac aaaaaagtgg tcgccgatcg tttagcggaa
     4261 tggaaaaaga caaatgaaca agtcaagaaa acaactgtct cttctcaaaa aaacgagtcg
     4321 acaggaacaa gtccttcaga ttctggcagt ggacaaacga taacgggaag tgaagaaaat
     4381 caagcctcaa ttattacgaa tgagtcatca caaggacaat atcaatccta tggacaatga
     4441 aaaa
//
LOCUS       DACJGB010000141         4433 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.147, whole genome shotgun sequence.
ACCESSION   DACJGB010000141 DACJGB010000000
VERSION     DACJGB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.147"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(543..2048)
                     /locus_tag="IYI34_002943"
     CDS             complement(543..2048)
                     /locus_tag="IYI34_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0560751.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IYI34_002944"
     CDS             complement(2045..2731)
                     /locus_tag="IYI34_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560752.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IYI34_002945"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IYI34_002945"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAR0560753.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACJGB010000142         4226 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.148, whole genome shotgun sequence.
ACCESSION   DACJGB010000142 DACJGB010000000
VERSION     DACJGB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.148"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..85)
                     /locus_tag="IYI34_002946"
     CDS             complement(<1..85)
                     /locus_tag="IYI34_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0560754.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            161..316
                     /locus_tag="IYI34_002947"
                     /pseudo
     CDS             161..316
                     /locus_tag="IYI34_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            335..1612
                     /locus_tag="IYI34_002948"
     CDS             335..1612
                     /locus_tag="IYI34_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0560755.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="IYI34_002949"
     CDS             1622..2278
                     /locus_tag="IYI34_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAR0560756.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="IYI34_002950"
     CDS             2278..3654
                     /locus_tag="IYI34_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAR0560757.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..4116
                     /locus_tag="IYI34_002951"
                     /pseudo
     CDS             3784..4116
                     /locus_tag="IYI34_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT         1662 a          480 c          745 g         1339 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatcgcag actgtagaca aaagctgttc
     4141 caaactcgta actcgagctt ggaacagctt tttatctact tcgtttcgaa tttttgaaca
     4201 aaaaaataaa ggctccttgc ctttcc
//
LOCUS       DACJGB010000143         4223 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.149, whole genome shotgun sequence.
ACCESSION   DACJGB010000143 DACJGB010000000
VERSION     DACJGB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.149"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(383..1669)
                     /locus_tag="IYI34_002952"
     CDS             complement(383..1669)
                     /locus_tag="IYI34_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAR0560758.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1662..2930)
                     /locus_tag="IYI34_002953"
     CDS             complement(1662..2930)
                     /locus_tag="IYI34_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAR0560759.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            3182..3643
                     /gene="mscL"
                     /locus_tag="IYI34_002954"
     CDS             3182..3643
                     /gene="mscL"
                     /locus_tag="IYI34_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAR0560760.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3702..4148)
                     /locus_tag="IYI34_002955"
     CDS             complement(3702..4148)
                     /locus_tag="IYI34_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560761.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT         1195 a          863 c          740 g         1425 t
ORIGIN      
        1 ccaaccaagt taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc
       61 agggcagctt tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat
      121 aagccgaaat atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
      181 agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag
      241 ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag
      301 aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag
      361 acttttcctc ataaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
      421 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
      481 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
      541 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
      601 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
      661 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
      721 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
      781 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
      841 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
      901 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
      961 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
     1021 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
     1081 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
     1141 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
     1201 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
     1261 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
     1321 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
     1381 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
     1441 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
     1501 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
     1561 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
     1621 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
     1681 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
     1741 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
     1801 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
     1861 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
     1921 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
     1981 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
     2041 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
     2101 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
     2161 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
     2221 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
     2281 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
     2341 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
     2401 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
     2461 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
     2521 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
     2581 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
     2641 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
     2701 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
     2761 tgatccggat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
     2821 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
     2881 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
     2941 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
     3001 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
     3061 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
     3121 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
     3181 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
     3241 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
     3301 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
     3361 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
     3421 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
     3481 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
     3541 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
     3601 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
     3661 agaaagccga ttttcagtgt cggctttctt tttttagatt cttaacttgc tacttgttcc
     3721 tttttcatgt cgatatgtgg aatatcatct tccaaataca catctgagat tgccttgaat
     3781 ccaaacgact cataaaaagc ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct
     3841 ggaaaatttt tctggatcat gttaatcgtt tcttgaacaa tctttcttcc taaaccatct
     3901 ttacggttgt cctgatgaac aaggacacgt ccaaaatgga tactctccgc ttcttcatat
     3961 actcgcgtgt aggcaaggat cttcccatca gtatcttgaa aaaacgtatg ccaagcttgt
     4021 ccatcaatgt catcaacttc ttgataagga cactgttgtt ccacaacaaa aactgcgatt
     4081 cgttctttga cgatcgcgta aaactctgct gttgacaatt cttcaaattt ctttacctga
     4141 tattccacta agcaaacgcc ccctgctttt ttattcttag cataccgatt cgcacacaaa
     4201 aaagcaaacg aaccttacga atc
//
LOCUS       DACJGB010000144         4151 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.150, whole genome shotgun sequence.
ACCESSION   DACJGB010000144 DACJGB010000000
VERSION     DACJGB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.150"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..106
                     /locus_tag="IYI34_002956"
     CDS             <1..106
                     /locus_tag="IYI34_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR0560762.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IYI34_002957"
     CDS             970..1182
                     /locus_tag="IYI34_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560763.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IYI34_002958"
     CDS             complement(1460..3259)
                     /locus_tag="IYI34_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAR0560764.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IYI34_002959"
     CDS             complement(3383..3979)
                     /locus_tag="IYI34_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAR0560765.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1226 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt t
//
LOCUS       DACJGB010000145         4132 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.151, whole genome shotgun sequence.
ACCESSION   DACJGB010000145 DACJGB010000000
VERSION     DACJGB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.151"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            412..591
                     /locus_tag="IYI34_002960"
     CDS             412..591
                     /locus_tag="IYI34_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560766.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            916..1179
                     /locus_tag="IYI34_002961"
     CDS             916..1179
                     /locus_tag="IYI34_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAR0560767.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            1173..1523
                     /locus_tag="IYI34_002962"
     CDS             1173..1523
                     /locus_tag="IYI34_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAR0560768.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1547..2161)
                     /locus_tag="IYI34_002963"
     CDS             complement(1547..2161)
                     /locus_tag="IYI34_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560769.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            2475..2897
                     /locus_tag="IYI34_002964"
     CDS             2475..2897
                     /locus_tag="IYI34_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560770.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            2907..3110
                     /locus_tag="IYI34_002965"
     CDS             2907..3110
                     /locus_tag="IYI34_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560771.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            3321..3905
                     /locus_tag="IYI34_002966"
     CDS             3321..3905
                     /locus_tag="IYI34_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560772.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1421 a          613 c          752 g         1346 t
ORIGIN      
        1 atcaacgatt tgaaaggaaa aaaaggcaat aatcatttat accgaaaagt gagaatgtgt
       61 gaaataagaa caaaaacctt gaaattaagg gaatggttct tatttcgcac caatcgctcc
      121 aaccgccccc gccgcttagg tggtgcgaaa taagcgggca aagcccgata acctaagaga
      181 aagaaggaga tccatatgaa taaaatcatt cctctgttgt tcgtactgtg tattggcgag
      241 atacttatgc gtcatggtgc agctgtacat ttaaacttcg tagtattctt aggaatacta
      301 gtattttgtt tgattttttg gcgctttgtg ttagcccttg tgagtctact attcaagttg
      361 attgcagcca tatttttcat agggttattt cgatgaaagg agagagtact aatgccaatt
      421 gcaataggaa ataaacggtt acccgtgaca ttagatgaaa agagacaaaa agaattgcag
      481 caactaaagc agaagtacgg aaaaagtgaa tctaagatta tgtgtattgc gttagatcta
      541 ttgatcgcgc aagaaaaagc aggatttgat ataccagcac taaaaaagtg acgtcacttt
      601 tcatttgaag aaacaaaagt gatagcactt aattgttcct actatttaga atattttcta
      661 tgtccttttt attccatttt gtatcaataa ctatgaaatc tttaaagtat ccagatcaat
      721 ttttttctta cctgattcta aagattttgt tttcttaaac atattatgat ttcctttcga
      781 ctattttcat ataaatgtta acatagttta tggattcatg gaatgttaat ctaatttaat
      841 agaaaaggat aattaagtat atacgttttt tcttatgtat gttatactat gtatatacag
      901 aaagaggtgt ctactatgga aatgttacct ataaaaaaat ggggaaatag taatggcctt
      961 agattgccaa agtatattat ggaatatctt ggaattcata cagaagacaa agtgaagata
     1021 atacaagaag aagtaaacgg tcagaagcgg ctaattatcg aagcggctaa tatagaaaat
     1081 gaattaacaa ttgaacagtt atttgaaaat tacactgatg aaagaaatca tgtgacgatt
     1141 caaaatttag gtgaagcagt aggtaatgaa aaatggtgag aaaacctaga caaggcgata
     1201 ttctattatt gaatacagca cctcgatctg gacacgaaca gacaggtaaa agaccttata
     1261 ttgttttaag tcatgatatt attgcggatt atagtaacgt tgttacagtt gctccaattt
     1321 ctagtacaac tagaaactat ccgctatatg taaatataaa ctcagaatat gaaatgaaga
     1381 caacaggaaa agtattatta gatcaactaa cgacaattga ttatgaagcc agagagtgta
     1441 tttttttgga aaaagcgcat gatagtttag ttgaggagtt attgatgaaa gttagaacag
     1501 tttttcaaaa agtaagcaaa taaaatagaa acagcagcat gatcaactat tcgtgattga
     1561 ccatgctgtc tttttttatt ctatatacgg tttgtctcgt aacattgtag tcttttgcaa
     1621 tcttagcgat cgcaacacct ttttttagat cctcaacgat gtttttataa acaagacgac
     1681 gttggggatc ttttgcattg gcactgtata gcttaggacg tcctttataa acaccgttcg
     1741 cttttgcaat cttaatgcct tgtgcctgac gtcgtttaga ttcggtacgt tcttgttcag
     1801 cgatcatggc caaaatttga atgatcaaat ctttaatgaa tttatccagg agaggattgc
     1861 caattgcttc ggccatgatc ggtagacttg tgatgatcag ctgcacattt ttcttcttca
     1921 agtaattgac tgaagcgatg atctcgtcgt aatttcttcc aagtcgatca atagcttcca
     1981 caataaaaat atcttgatct cggacgaaat ccagtgcttc ttgaaaaaca ggtcgatgag
     2041 taatcgttgc gccagattgt ttctcaatga aaattctctc tgcaccgaat tttttcattt
     2101 cttcaatctg ccgttgttca ttttgatcga tggatgagac acgtatgtat gcaactctca
     2161 aaatttttac ctactttctg caactattta aaaaacaccc taatcataca cgagaaaacc
     2221 agatttttca atgtcgcggt cgtgtctagt tagggtatac cttatataga caaaaactaa
     2281 tgatggagga aatagtatgt gtcacttttc tttttaaatg gcaatatttt ttgtaaataa
     2341 atgtcaatca agaaaattta aacgattatg acattaaaaa tatattttat gaattttcta
     2401 gtatataact tgatttatct gttaagattt attaagaaaa cgttttctta tttttgattt
     2461 cgaggtgata gacaatgaaa aataagttta ttttatcttt aggtatttta actattggaa
     2521 ttagtgtaac tggaactaca attcctacat ttgctgatag tacaaatgaa ccaactactg
     2581 tagtagataa taaccaagaa ttaactcctt tagataggaa cggaatagcc attggtaacc
     2641 caactaacga cttaactgtt actgaaaatt atttaatagt ttctccatta cttgctcgag
     2701 cagcttctaa aacgatacga gtgacaggaa cgatgatttt tgataaagtt ggttggcctt
     2761 ctagacctcc tcaatcacgg aattggaaag aaaaaagaaa tggttattgg tactatggaa
     2821 aattgtattt acaaagttat aaatcagctg gaaacaattg gattgcgact tattcaggaa
     2881 ccttaactag aaagtaggga ttttttatgc cagaatttgt gaacacacca aatttttatc
     2941 aagatagcga tattctaagt aaaattaatt taccgaaaga agaatcagct ttacaagttc
     3001 agcgaacttt acaggagaca caaaaaattc aaaaatcaat ttctaatcta gaagattctg
     3061 aagaaaaaag aaaaaaaaga gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa
     3121 aaaagagtgt ctagtcgaca ttcttttttt ctaattgaaa gtgttcataa aatcacccat
     3181 ttctggacac tttctttttt tgctttattt ttcaaaaaaa caattcattt catgttcata
     3241 aacccataac aatatctatg ttcagttaaa ctatatggat aaagttatgt tacaataaaa
     3301 ttaatgaagg agtgattttt ttgaaaatag gatacgcccg ggtatctact ggtttacaaa
     3361 atttggattt acaagaagac agcctaaata aatttggttg tgaaaaaatt tttactgatc
     3421 atatgagtgg ggcaaaaagt aatcgaccag gattagaaat ggccattgat tttgttcgct
     3481 ctggcgatac acttgtcgtt tggcgcttag atcgtttagg tagaaatatg gaagatttga
     3541 tttcaatcgt caatcgactg aatgagcgtg gtgtgagttt tcatagtatt caagaaaata
     3601 ttacgatgga caaatcaagt tctacaggac agctcatgtt tcatttgttt gcggcatttg
     3661 cagaatttga gcgtaatttg atcctggaac gatccgcagc aggacgagca gctgctcgtg
     3721 caagaggacg gtttggtggt cgtcctgaaa aattgtctga acaagatttg gaattactga
     3781 agactttagt ggatagcggt acacctatta agactattgc ggaacggtgg aacgtctcaa
     3841 gaaccaccat ttatcgttat ttggcaaaaa tggaggagaa aaatggaaag acaaattctc
     3901 attagttttt gtcatttaat gctgaaaatt tttgccattt atctagaaaa gtgacaatag
     3961 tatgagaatt ttttgtaagt gtctactgat aacttattct caaatgttgt aaggatttta
     4021 ttcatttgtt aatattaaag aacggaactc caaaaaagtc cgtactctaa tttgcagtct
     4081 ctcactcttt tagagtgaga gtttttatta gattttcaaa ttaattagta aa
//
LOCUS       DACJGB010000146         4117 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.152, whole genome shotgun sequence.
ACCESSION   DACJGB010000146 DACJGB010000000
VERSION     DACJGB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.152"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..435)
                     /locus_tag="IYI34_002967"
     CDS             complement(<1..435)
                     /locus_tag="IYI34_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0560773.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREA"
     gene            complement(843..1547)
                     /locus_tag="IYI34_002968"
     CDS             complement(843..1547)
                     /locus_tag="IYI34_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560774.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(1734..1979)
                     /locus_tag="IYI34_002969"
     CDS             complement(1734..1979)
                     /locus_tag="IYI34_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560775.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(1996..2847)
                     /locus_tag="IYI34_002970"
     CDS             complement(1996..2847)
                     /locus_tag="IYI34_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAR0560776.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            3013..4017
                     /locus_tag="IYI34_002971"
     CDS             3013..4017
                     /locus_tag="IYI34_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAR0560777.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1263 a          719 c          795 g         1340 t
ORIGIN      
        1 tgcttcacgc aagatccgtg tcaccgtccc atccgagacg aagtacttct tagcgattag
       61 tttacgtgat tgattttcgt acagttccat gagaatcgcc tgcttcagtt cctttgttag
      121 ttgatgatgt tcatcaacta agtccgtttt cgcagaaaag gtccgtccac aatcatggca
      181 gagaaaacgg gtacgtttta attctaaaat agtcaacttt tctttgactt tcaacatctg
      241 agtgcgtgtc gtataggaac cgttgcggat aatacgaatc gatccacagt gttcacaatg
      301 ggaaggtgag tcatccaatc tccctttaat aaactgcgct tgaacgcctt tatattcttt
      361 ttcaatcagc cattcttcat caaaaaataa atgtttatct gttaatccga gtaattttct
      421 agtatgatta tccattgaga gactcctttg gtagggtgac gttttggtcg actaaatttt
      481 accaaaagtg agtctctttt tgagttgaaa cgaagaaaaa agtgttagta gaaaatcgct
      541 ttcgattttc caccaacaca aaatattata gaaccgcttt tttctctatc agaaaatatg
      601 tttattatta gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa
      661 gatatcttca ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga
      721 caggtcaagc tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa
      781 attattatat gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat
      841 ttttatcgat cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat
      901 ttgactttgt atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata
      961 gaaatagttg ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt
     1021 tcattttgtt ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa
     1081 cttaaagcgg cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt
     1141 tccgtttttt cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc
     1201 gcagttggtt catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga
     1261 agattgtcac ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca
     1321 gtattgaagt tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct
     1381 aatccgatcg ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca
     1441 taacgtcgca agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat
     1501 gaaggacgga attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt
     1561 cgctcatacc ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa
     1621 aacttgcgta tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga
     1681 aaaataaaaa atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt
     1741 ttcggtatca aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag
     1801 ggtttgacca ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt
     1861 gtccagtttc ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg
     1921 gaatgaaaag tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata
     1981 aaatcacctt tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc
     2041 atctaactga tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt
     2101 attttctttg attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag
     2161 cgaccaacgc aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat
     2221 ttcaggcaca tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc
     2281 atgttttttt gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg
     2341 attgaccatc ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa
     2401 attgctcaca ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca
     2461 tgtatctgca tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat
     2521 caaaaacaga tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat
     2581 cacaagttca tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct
     2641 aggaattcca ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc
     2701 agtatcgata tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc
     2761 cccatcaggt gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag
     2821 acgataagtg cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata
     2881 agtgtagcgc atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt
     2941 taaaagaaaa tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa
     3001 gggaatgaga aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa
     3061 actggttctc cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat
     3121 tttcttcaaa agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca
     3181 gaagaaagca tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt
     3241 gaagcaatcg ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca
     3301 gatgtatttg ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc
     3361 aattatcgtg aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc
     3421 ttgattttga atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct
     3481 gtgaatttga ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa
     3541 aaagagcttt atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa
     3601 aatgtaaaag tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa
     3661 tttgatatca tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg
     3721 ttggatcatg agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct
     3781 tttaaagaag aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta
     3841 caagaaattt ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa
     3901 ctgatcgtac cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg
     3961 gatggtacga tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa
     4021 tgactagaat attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa
     4081 ggatcggaaa agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DACJGB010000147         4105 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.153, whole genome shotgun sequence.
ACCESSION   DACJGB010000147 DACJGB010000000
VERSION     DACJGB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.153"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..357
                     /locus_tag="IYI34_002972"
     CDS             <1..357
                     /locus_tag="IYI34_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HAR0560778.1"
                     /translation="LNNDLADVDCENWEEDTPFKDPRELYDFLKTEKPEEELVFSHGD
                     LGDSNIFVKDGKVSGFIDLGRSGRADKWYDIAFCVRSIREDIGEEQYVELFFDLLGIK
                     PDWEKIKYYILLDELF"
     gene            complement(465..653)
                     /locus_tag="IYI34_002973"
                     /pseudo
     CDS             complement(465..653)
                     /locus_tag="IYI34_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            633..1205
                     /locus_tag="IYI34_002974"
     CDS             633..1205
                     /locus_tag="IYI34_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0560779.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            1545..2399
                     /locus_tag="IYI34_002975"
     CDS             1545..2399
                     /locus_tag="IYI34_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAR0560780.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMIERI
                     IQLENEKE"
     gene            2498..2707
                     /locus_tag="IYI34_002976"
     CDS             2498..2707
                     /locus_tag="IYI34_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAR0560781.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            2724..2996
                     /locus_tag="IYI34_002977"
     CDS             2724..2996
                     /locus_tag="IYI34_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAR0560782.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            2998..3861
                     /locus_tag="IYI34_002978"
     CDS             2998..3861
                     /locus_tag="IYI34_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAR0560783.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
BASE COUNT         1511 a          637 c          804 g         1153 t
ORIGIN      
        1 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaagacac tccatttaaa
       61 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
      121 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
      181 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
      241 tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
      301 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagtac
      361 ctagatttag atgtctaaaa agctttaact acaagctttt tagacatcta atcttttctg
      421 aagtacatcc gcaactgtcc atactctgat gttttatatc ttttctaaaa gttcgctaga
      481 tagagttcta tattagagat gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt
      541 ataaccaggg tgcaatgaga agcactaaca gcagctagct taagaacgca taggatatac
      601 tttttatgga agtcacagag gaggaataaa atttgaacag gattaacaga gtaacctcta
      661 ttcttattca gctgcaatca aaaaaaataa ttcctgccaa agaaattgca cagcgattta
      721 atataagctt aaggacagtt tacagagata tccggacact tgaagaagcc ggaataccaa
      781 ttggatctga ggccggaaaa ggatattttc ttgtagaggg cttcctactt ccgccggtaa
      841 tgtttacagc ggcggaagtg ggtgcattga ttacagcagg gaaattttta aattgccatg
      901 gagatgaatc atttataaag gattttgatt cagctatgta taaaatcaaa tctattttga
      961 agcatggtga aaaaaactat gcacaggagt tggagaacag tattaacgtg tacagcacat
     1021 ctggacagaa aaatacattg gcggataacg ttattgcagc aattcagact gcaatctgca
     1081 ataaaagggt aatatcaatt caatatcctg catcaggagg gcaagaaccg gaaagcagaa
     1141 tgatagagcc tatatcactg gggttttatg aacagaactg gtacttaatc ggttttgcag
     1201 gctaagaaat gaatatagga attttcgtgt ggatcggatt aaaagcattt gcatagaaga
     1261 agagctctgt caaagccatg atggctcttt ggaacaaata ttaaaacaaa tgctttcata
     1321 taaaaaacta tatgccctac aatgccgttg taaaaattgg ggaaaaggga tatattgatt
     1381 ttatatcttt ctcaaaataa atataaaagc cctttaaaga gggcttttat atattaatca
     1441 caaatcactt atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgattct
     1501 taatgataca atattactat acaaaaaaag aatggggcgt agttatggag aaggaaaaac
     1561 taaaaatact tgaagaatta agacgtattt taaacaataa aaatgaagca attattatct
     1621 tgaataatta ctttaaaggt ggtgttggaa agtccaaatt atcgactatg tttgcttact
     1681 tgacagacaa attgaattta aaagttttaa tgatcgataa ggacttacag gcaacattga
     1741 caaaagactt agcaaaaact tttgaggtag aattgccacg tgtcaatttt tatgaaggct
     1801 tgaaaaatgg aaacttggct tcttctatta ttcatttgac tgataattta gacttgatcc
     1861 ctggcacgtt tgatttgatg ttactgccaa aattaactcg ctcatggacg tttgaaaatg
     1921 aaagtagatt gcttgctact cttttagcac ctttaaaaag tgactatgat cttattatta
     1981 ttgatactgt accaacgcca agcgtttata caaataatgc aatcgtggcg agtgattacg
     2041 ttatgatccc tttacaagca gaagaagaaa gtacaaacaa cattcaaaac tatatttcct
     2101 atttgattga tttgcaagaa cagtttaacc ctggactaga tatgattggt tttgttcctt
     2161 atttagttga tacggacagc gcaacgataa aatcaaacct ggaagaactg tacaaagaac
     2221 ataaagagga taatttggtt ttccgaaata ttatcaagcg aagtaataaa gtaagtactt
     2281 ggtctaaaaa tggcattaca gaacacaaag gctatgacaa aaaagttttg tctatgtatg
     2341 agaacgtatt ttttgaaatg attgagcgaa tcattcaatt agaaaatgaa aaagaataga
     2401 atcacaaatc acaagtgatt aatcacaaat cacttgtgat ttgtgatagg tgatgataaa
     2461 ataaatagta agaagaaata gaaagaagtg agcgatcgtg ggaaatttag gcgcacagaa
     2521 agcaaaacga aatgatacgc caatcagcgc aaaaaaagat ataatggggg ataagacggt
     2581 tcgtgttcgt gccgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa
     2641 cgtaaaagaa gttatggatc aagccttaga agaatatata cggaaatatt tacctgacaa
     2701 actttaaaag gagtgaaaat gaaatggcag ttacgtatga aaaaacattt gaaattgaaa
     2761 tcattaatga gttatcggca agtgtttata atcgggtatt aaattatgtt ttgaatcatg
     2821 aactagatac taaaaatact cgtttactag aagtgaatct tttaaatcaa ttagaagtgg
     2881 cacaagaagt tgatttattt caacaaccat ttgaagaatt acaagctatt catgagtatt
     2941 ggcggtcaat gaatcaatat tcaaaacaaa ttttgaataa agagaaagtg gcttaacatg
     3001 gcgaatatta ctgacttcac cgaaaagcaa tttgaagatc gtttagaaaa gaatgttgaa
     3061 cgactaacta aaaatagact agcggttgaa tcgccaaccg cttttttact tggtgggcaa
     3121 ccagggtcag ggaaaaccag tttgcgatca gcaatttttg aagaaacaca agggaatgtt
     3181 attgtcattg ataatgatac ctttaaacaa cagcacccaa attttgacga attagtgaaa
     3241 ctctatgaaa aagacgtagt aaaacacgtt accccttatt cgaatcgcat gacagaagca
     3301 cttattagcc gtttaagcga tcaaggctat aatttagtga ttgaaggaac aggacgaaca
     3361 acagacgttc ccattcaaac agccacaatg cttcaatcta aaggttatga aacaaaaata
     3421 tacgtcatgg cagtaccgaa aattgaatca tacttaggaa cgattgaacg atatgaaacc
     3481 atgtatgcag atgatccaat gacagctagg gcaacaccaa aacaagcgca tgatattgtt
     3541 gtcaaaaact taccgaccaa tttagaaacc cttcataaaa cgggcttatt tagcgatata
     3601 aggctttaca atagagaagg agtgaaactc tattcaagct tagaaacgcc ttccattagt
     3661 ccgaaagaga ccttagaaag agaattaaat cgtaaagtat cagggaaaga aattcaaccg
     3721 actttggaga gaatagagca aaaaatggtt caaaatcaac accaagaaac ccctgaattt
     3781 aaagcaattc aacaaaaaat ggaaagcttg cagctaccta caccaccaat acccaaaaca
     3841 cctaaacttc caggtattta aaccttaaaa aaggaaaaga gtagttacca aaaaacggta
     3901 actactcttt ttttataaaa acatttcctc atgttggggt cccgagcgcc tacgaggaat
     3961 ttgtatcgat aagaaataga tttaaaaatt tcgctgtttt tttctaattt cattttgagg
     4021 tgaattaatt ggtagttatt agaggtgccc tatacctaat aatttatcta cattcccttt
     4081 agtaacgtgt aactttccaa attta
//
LOCUS       DACJGB010000148         4067 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.154, whole genome shotgun sequence.
ACCESSION   DACJGB010000148 DACJGB010000000
VERSION     DACJGB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.154"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            86..1435
                     /locus_tag="IYI34_002979"
     CDS             86..1435
                     /locus_tag="IYI34_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0560784.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="IYI34_002980"
     CDS             complement(1563..2153)
                     /locus_tag="IYI34_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAR0560785.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="IYI34_002981"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="IYI34_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAR0560786.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1173 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaaa
//
LOCUS       DACJGB010000149         3959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.155, whole genome shotgun sequence.
ACCESSION   DACJGB010000149 DACJGB010000000
VERSION     DACJGB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.155"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(609..1166)
                     /locus_tag="IYI34_002982"
     CDS             complement(609..1166)
                     /locus_tag="IYI34_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560787.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1345..1956)
                     /locus_tag="IYI34_002983"
     CDS             complement(1345..1956)
                     /locus_tag="IYI34_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560788.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2069..2326)
                     /locus_tag="IYI34_002984"
     CDS             complement(2069..2326)
                     /locus_tag="IYI34_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560789.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2923..3201)
                     /locus_tag="IYI34_002985"
     CDS             complement(2923..3201)
                     /locus_tag="IYI34_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560790.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3235..3486)
                     /locus_tag="IYI34_002986"
     CDS             complement(3235..3486)
                     /locus_tag="IYI34_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560791.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1200 a          810 c          526 g         1423 t
ORIGIN      
        1 tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa tggtgtccaa
       61 aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat tatctccacc
      121 aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat tttcatacaa
      181 aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac tagtatccca
      241 atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct tttatgtatg
      301 ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa caagttgttt
      361 ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa tgcatacgca
      421 tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc tcagctaata
      481 gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat ttcaatggtt
      541 ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa tggattagta
      601 gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact aataagacgg
      661 attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc tatttcaggt
      721 gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt aggattaggc
      781 tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac ataatatatt
      841 cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc acttgctaaa
      901 tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat
      961 aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata ttctaatcta
     1021 aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa tattattcta
     1081 cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat gattaatatt
     1141 ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc tcacatatta
     1201 gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt cgctatgaat
     1261 tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat gatttggctt
     1321 gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg gttcagatat
     1381 ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt ctccgaaatt
     1441 aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc ttttaaaacc
     1501 ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta cattttcccc
     1561 catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc caaaaggtag
     1621 atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca tagaacctgc
     1681 gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt taatctgtcc
     1741 atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg gttcgggtat
     1801 tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca aaaaaactcc
     1861 tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc tcattgatcc
     1921 attgtattcc attttattcc ccctattact attcatatcc gcctatctat atcgatcttc
     1981 caatgcagag ataattgctt ccataccttg attttttact gagttaccta tgcgagatat
     2041 cattctccac taaggtgcat catattgtct atctaacatg attaattctc caataggtgt
     2101 atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct accatgtttg
     2161 ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct ggattaaata
     2221 taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct tttgaatgtc
     2281 cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt taccctgacc
     2341 attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc tcctaacttc
     2401 actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc atttaattcg
     2461 gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt atcaataaac
     2521 tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca taatatcatt
     2581 gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat tgacgaaaaa
     2641 gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa ctatttgtaa
     2701 tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga ttcccaagca
     2761 aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc ttatataaat
     2821 tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg cttatatcac
     2881 aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt tttcaaattg
     2941 tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt taacattatc
     3001 atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta taattaacct
     3061 tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat ctctcctaat
     3121 ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat acatactgcc
     3181 tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc gttcttactt
     3241 ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca acaataagaa
     3301 tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat aagtaggatt
     3361 agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag cacaaacacc
     3421 aaagataaca actccaaaca ttgaattttt atacaatgac catttattta taggcttttc
     3481 tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag ttcataagcc
     3541 ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc ttgttttaag
     3601 cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg ccgaaaagtc
     3661 tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac tatttgtgaa
     3721 acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag tagctcttaa
     3781 ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat cccctctaaa
     3841 aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa tagagcatta
     3901 ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca attatttgt
//
LOCUS       DACJGB010000150         3935 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.158, whole genome shotgun sequence.
ACCESSION   DACJGB010000150 DACJGB010000000
VERSION     DACJGB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.158"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            453..1142
                     /locus_tag="IYI34_002987"
     CDS             453..1142
                     /locus_tag="IYI34_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAR0560792.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1129..2337
                     /locus_tag="IYI34_002988"
     CDS             1129..2337
                     /locus_tag="IYI34_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAR0560793.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2631..3902
                     /gene="serS"
                     /locus_tag="IYI34_002989"
     CDS             2631..3902
                     /gene="serS"
                     /locus_tag="IYI34_002989"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAR0560794.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1285 a          677 c          791 g         1182 t
ORIGIN      
        1 tatcgtttgt tatttgtcaa gaactttttt gaagtttttt tcgatagaat caagcttcgc
       61 aagtggttct tttttaacga ctcagttatc ttatcatgtc atttccaaca tgtcaacaac
      121 ttatttttta ttatttaata agttattggt gttcttttga acaaataata atatatcaac
      181 atcacatgtg aaagtcaata agaaaatgct tatttatcaa catttctagc ttacatgatc
      241 attggttagt tctggtcctt tttcgttcgt ctttataact ccgagagacg taaaatcctt
      301 ttgttttcag tttaatgttc atgttcttgt cttattcaca ccaactagat gtctcacctt
      361 ctaaaatttt ccttaacaaa gtgctgcttt tatctatttc tgcttttttt tgctagaata
      421 atagtaacaa tatgactctg gaggatttat ttatgaaaat tttgattgct gacgatgata
      481 aagaaatcgt tgaattatta agtatctatg ttcacaatga ggggtatgaa gcagtaaagg
      541 cttatgatgg gaaagaagca ttatctaaaa tcagaacaat ccctgatatt gaattactta
      601 ttttagatat catgatgcct gagatggatg ggatgcaagt agtgaaagag ctacgaaaag
      661 aatcccaaat accaatcatt atgctgacag caaaaactac tgatatggat aagatcaaag
      721 gactggtcgc tggtgcagat gattatgtga ccaagccatt taatccgtta gaagtaatgg
      781 ctagagtaaa gtctttatta cggcgtacaa aaatgcagat tgccccagat cagcctgatg
      841 tattagaagt aaatgcatta atcatcaata aagactctca tgaagttaaa acaatcgatg
      901 ggaaagaaat ccagttaaca gctttggaat tcggtatcct ctacttgctt gctagtcatc
      961 ctaatcgagt atttagcgcg gatgaaattt ttgagcgtgt ttggaaacaa gaaagtattg
     1021 tctctgctaa aactgtcatg gttcacgtta gtcatttaag agataagatt gaagaagcaa
     1081 ctggtggtga aaaagtcatt caaaccgttt ggggagtggg ctataaaatt gacgcaagat
     1141 agtaaacgag caactgaaaa acgccgtcgg atcaatttga cttcaaaaga aatcagcgaa
     1201 ttgttagcgg aaggaatcat cacgattatc cttttgcttc tattaaatgt ttctattctg
     1261 gttgttatca gttcggtgat caacagttca ccttctttga cgaatgcgat ttgggattca
     1321 aagaatattt ttgcagaacg attgaacact gatttgttct ggaacggccg gaattttatt
     1381 attccctttt tctttctatt agatattggt gttttgtatt ggcgcttgat tcgaagatat
     1441 cgccaaatgc aattgcggca tatcatcagc gaacttcact acatcgcaaa cggaaattat
     1501 gatcatcgta tcccttttga actgagtggt gacttaagcc gagtggtaac aagtatcaat
     1561 ggattggtgg atagtaccgt agcagcgatt gaagacgaac gtaaaatcga aaaatcaaaa
     1621 gatgaattga tcacgaatgt cagtcatgat atccgtaccc cactcacgtc catcattgga
     1681 tacctaggac tgatagaaga tggccagtat cactcagaag aagatctttt aaagtatacg
     1741 catactgctt atatcaaagc aaagcagatg aaatctttag tggacgactt atttgaatac
     1801 actaaggtac gccagcctgc tgtcccggtg aatttttctg cctttgacat gatccagttg
     1861 atcgaacagt tagcagctga ttttgagttg gaagcttcta aaaaaaatat ccaaatactt
     1921 gttcaatcaa aagtagactc ccttatcatg gatggcgata ccgaaaaact tgtacgtgtc
     1981 ttcaataatc ttttgacgaa tgcgttgaaa tacggaaaag gagcaacgaa gatcgtaatc
     2041 gaagtagaac gtatcggttc agaagttgtt gcaacagtca aaaataacgg agcaatgatc
     2101 ccacaacagg caatcgataa tctattcgac cgtttttatc gtgtagaaga atctcgttca
     2161 caagcaactg gaggaactgg acttggttta gcaatcgctc aaagcattgt ggctttgcac
     2221 ggcggataca tttatgcaaa atcagataag caatggactt ctttcatcat gcaccttcca
     2281 atcaagaaaa atgaaaaatt acctattgat acacaggaag ttattgacga aagctgaaat
     2341 attcgctatt cttattacaa ttattagaat aaatgaaaaa cacagaaccc agtacacttt
     2401 attctttttc agagagctga tggcaggtgt aaatcagcaa aggataattt ggaagttcca
     2461 gttcgtttac gtgccctttc gaaataagaa aggtcggttc aacagccgtt atgtgttcaa
     2521 caagtgcagc tgacgctgaa cttgggtggt accgcgaatt tcagactgat tcgtcccaag
     2581 aataatggga tggatcagtc tttttttatt ttcaaaaagg aggaaatcat atgttagatg
     2641 taaaaatgat gcgacaaaat attgaagaag tacaagaaaa attgatgact cggggcgtaa
     2701 aaaaagaaac attagctgaa ttcatggaac tagatgaaaa tcgtcgtaag ctactagtca
     2761 aaagtgaaga attaaaaaaa tatcgaaatg atgtttctgc tgagatcgcc caattgaagc
     2821 gtgcaaaaga agatgcttct gctaaaatcg ctgaaatgaa agaagtcggt ggcaacatca
     2881 aagcattgga tactgaaatt gctgaagtgg atgaaaaatt gaaacaaatt gcaactactt
     2941 tgccaaacct tccccatgat tctgtgccag ttggaacaga tgaagaggac aatgtagaaa
     3001 tccgtcgttg gagcgaacca cgtacatttg cttttgaacc aaaaccacac tgggaaattg
     3061 cagaaaatct tgatatttta gattttgaac gtggcgcaaa agtagccggt agccgttttg
     3121 tctattacaa aggattaggt gctcgattag aacgtgctgt ctacaacttc atgcttgatc
     3181 agcatattta cgaacacggc tatactgaaa tgatcactcc ttatatcgtc aacagtaaag
     3241 cgatgtttgg tacgggacaa tttccaaaat tcaaagaaga tgttttccaa ttggagaata
     3301 ctgatctaac attaatccca acagctgaag taccattaac taactattat aatgatgaaa
     3361 ttttagatgg tgcagattta cctatttatt tcactgcact aagccctgct tttcgttctg
     3421 aagccggaag tgctggtcgg gatacacgag gactgattcg tcttcaccaa ttcaataaag
     3481 tagaaatggt caaattttcg gatgtagagc attcttacga agaattggaa aaaatgacag
     3541 ctgatgcaga agatatcttg aaaaaattgc atttgcctta tcgtgtgatg gccttatcaa
     3601 ctggagacat gggcttttct gcagctaaaa cttatgactt agaagtatgg atacctgcac
     3661 aagatactta tcgtgaaatc agttcttgtt ctaactgtga agatttccaa gcacgccgcg
     3721 caatgatccg ttatcgcgat aatgatggaa aaatccacta tactcatact ctgaatggtt
     3781 caggacttgc agttggccgt acagtagctg ccattttgga aaactaccaa aatgaagatg
     3841 gttctgtcac agttccagaa gcgctcgttc cttatatggg gaatctgaaa gttattaaat
     3901 aaagaggctg tgaaaaagcc gcttagtttc gagta
//
LOCUS       DACJGB010000151         3910 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.159, whole genome shotgun sequence.
ACCESSION   DACJGB010000151 DACJGB010000000
VERSION     DACJGB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.159"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            4..1872
                     /locus_tag="IYI34_002990"
     CDS             4..1872
                     /locus_tag="IYI34_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0560795.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            1865..2356
                     /locus_tag="IYI34_002991"
     CDS             1865..2356
                     /locus_tag="IYI34_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAR0560796.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            2344..2898
                     /gene="srlA"
                     /locus_tag="IYI34_002992"
     CDS             2344..2898
                     /gene="srlA"
                     /locus_tag="IYI34_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAR0560797.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            2916..>3910
                     /locus_tag="IYI34_002993"
     CDS             2916..>3910
                     /locus_tag="IYI34_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAR0560798.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1361 a          630 c          797 g         1122 t
ORIGIN      
        1 cacgtgacgc tagtaaacag atggtaccaa ttattgaaat tactagtcac tcacaagaaa
       61 gtttcaatcc aggaagtgca aaaagaaatc aaaaccagta atcaaaccat aaaaaaagat
      121 attggggagt tgaatgaaga aattgccggt attgcaaaaa tcatccaaaa aaacaaacat
      181 tttgaacttg aaatttatga ttttgatttt tttgatgaga tcatgcaagg aaaattgaaa
      241 acagccagtg atttcaattc ggcatccaaa agaatcgctt atttactaaa taggctgatc
      301 gaaacagagg atttcttacg tatggatgat ttgtctgaag aagtcggtgt cagcagagga
      361 acagtggtca aggatctaaa gacattaaaa gagatgatca atgattttga tgtaaagatt
      421 acaggcacac caaataaagg actaagaata ataggagatg agctagaatt acgactactt
      481 ttattttatt ttgtttctcc ctacttttca gagacgaaaa cagaacagtt tctagaagaa
      541 aaacaagcaa tcaaacattt tcagaagaag acgaatgcta gtaaacaaga aatcaccctt
      601 ttaacgaaag ttatcggtat ttctttgaat agaatctcta gtaactatct gttgaagaag
      661 cccatcaatc gttacagtgg gtgttttgaa tatatccaag aattcaatga actgatcaat
      721 cagttggaag aaatctatga actaactttg agccaatatg aaaaagattt tatcagtttt
      781 cctttgaaca tcatcaacac aagtataacg atgataagga aagctaactc tgatttgcgc
      841 ttatatttcg accccatgat gatgcgtatt cgacgattag atctcattga tctggatgag
      901 tccttcttat atcaagagat gaaacagcac ctctctttat tgataaatcg ggtaattttt
      961 cgttatcgat tgactgattt attttatggt gagatagaaa aaaagtatcc tttttcttac
     1021 caaatcgcta gcgaatgcat acaagcatta gaagctatct tgcaacataa gatctctaaa
     1081 gtagagatca gctacctaac gttgtatttg gagttagcat ttcgatatca aaaagagcat
     1141 ttttcctcta aaaaagtagc cattgttcat acgacaggaa aaggaacagc attaatgatc
     1201 caaagacaaa tcaaaaggat attggggagg gatatacaaa taacacatgt ttcagaagaa
     1261 caatatgaaa aagtagatct aaacaaatat ttcgctgttt ttacaacgat tccgttaaaa
     1321 aatgtaaatg aagagacacc ggtgatccat ttgactaatc tttttgatga ctcttggtta
     1381 agggcacaat ggcaaaaagc aaaaaaaaag cgattagccg ggtttcattc tactgttttt
     1441 ttctatcaaa tatggaaaga aaaaaagacg tatcaagaat atttaaacaa attagtcgct
     1501 cctttgatca gaggctatta tgtggatgaa gaatttgaaa aaaggatctt gaaaagagaa
     1561 tatcagcaaa gcacatttgt taacaaagaa tttgcttttc ctcacacaac caaccagcta
     1621 tctcaacaca ttgttttatc agtagggcta ataactgatc aaggtttagc agaaacagga
     1681 ggaaatggag ttattttttt atttgccata cctgaagaaa tgacggctga agttgagaat
     1741 gaattatttg agctatacga tatcctattt gaactcataa aaatgggaat agatgagttt
     1801 attgaattag caccgacaca agaatcattt ttaaaactaa tcaaaagtaa aggagagaga
     1861 cttcatgaat gatatgtatc tattcatagt tttcgcagct tcagcttatg tgctgcaagc
     1921 agttatcagt tggaagcaac taaagcattt caatcaaaca tatagcgaac tgcgaagaaa
     1981 aggaaaagta gcgattggta aacgacctgg gaaaatccgt tcaggcacga ttgttatgct
     2041 agctatagac gatcatgatc aagttttaga agtcagaaaa atgcaagggg ttacatcttt
     2101 ggcaaaattt cattcattac ctgtctatgc gggtgaagat atccattaca tcgatcgtta
     2161 ccatccctca gttcgagcgg aagatcgttt gacacaagca gctttagaaa atgccagaga
     2221 agtatactta cgaatagagg caggaaatga aaaaagtgaa caaccttcat tgccttttat
     2281 tgctatgctc aataattttc ggatatggaa aagtacgatc aaagaaaaaa ggagtgacaa
     2341 acgatggata tcataacaaa atttgctgag ggatttatga ggctgttcca aacaggtgca
     2401 gaaacattca taagctggat gacaggaatc gtaccagttg tcttgatgct gatggttgca
     2461 atgaatacgc tgattgcttt tttaggtgaa gaccgagtaa cgaaagtagc caaattatca
     2521 gcgaaaaatc cgcttactcg ttaccttgtg ttaccgttct tatctgcttt tatgttggga
     2581 aatcctatgt catttacaat ggctcgtttc cttccagaat actacaaacc aagctattat
     2641 gctgctcaag ctcaattttg tcatacgagc aatggtgtat tcccccatat taatcctgga
     2701 gaattgtttg tatggatggg gattgctcaa gggatcgaaa ccttagggct aaattcgatg
     2761 gagctggcta tccgatactt gcttgtggga ctgctgatga actttattgg tggttggata
     2821 actgatttta ctactggatt tgtatgcaga cagcaaggaa tcgttcttag caaaaaagta
     2881 gagctttcag ttgattagag aaaggagagt tttaaatgta tggtgatatt aagatcgtaa
     2941 gaggaaacgg cgggtatgga ggtcctttga ttattaggcc gacaaaagat aaacacaagt
     3001 tcatctatgt cacaggtgga ggtgagaggc caatgatcgt agataaaata gaagaactga
     3061 caggtatgga gtctatcaat gggttcaaaa catcaatacc tgatagtgaa atcgcgcttg
     3121 caatcgttga ctgtggaggg actcttagat gtggaattta tcctaaaaaa gggataccaa
     3181 ccatcaatat cgtttccaca ggaaaaagtg gcccattagc acaatatatc actgaagata
     3241 tttatgtttc agcagtaggg ttagaacaaa tacagccact aatggaaact gatgataaca
     3301 aagaaacagt aaatactgca tctccagata aaaagtacaa atatgatact tctaaaaaga
     3361 taaccgagca aaaagcagag cagcaaaact ggatagcaaa aattggaatg ggcgcgggaa
     3421 aagtagttgc tacattcaat caatcagcaa gagaagcagt agatacgatg atccggacag
     3481 tgatcccttt tatggctttc gtttcccttt tgattggtgt gatacaaggt tctgggatcg
     3541 gtaatgtatt tgctaacatt atggcacctt tagcaggaaa tgcagttgga ttaacaatca
     3601 ttggctttat ttgttcactg ccatttttat ctcctttgct tggtccaggg gcagttatta
     3661 gccaagtgat tggaacattg atcggtgtag agattgggaa aggaaatatt cctccccaat
     3721 tagcattacc ggcactattt gctatcaata ctcaaaatgc atgtgatttt attccagtcg
     3781 gtctaggttt ggaagaagca aaagctgaga cagtagaagt aggtgtacca tcagttcttt
     3841 attcaagatt tcttaacggc atcccacgag ttatcattgc ttgggtagca agttttggat
     3901 tatatagctg
//
LOCUS       DACJGB010000152         3763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.160, whole genome shotgun sequence.
ACCESSION   DACJGB010000152 DACJGB010000000
VERSION     DACJGB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.160"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            288..554
                     /locus_tag="IYI34_002994"
     CDS             288..554
                     /locus_tag="IYI34_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560799.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            612..1124
                     /locus_tag="IYI34_002995"
     CDS             612..1124
                     /locus_tag="IYI34_002995"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0560800.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="IYI34_002996"
     CDS             1121..2158
                     /locus_tag="IYI34_002996"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAR0560801.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            2318..2494
                     /locus_tag="IYI34_002997"
                     /pseudo
     CDS             2318..2494
                     /locus_tag="IYI34_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            2549..3763
                     /gene="aph(2'')-I"
                     /locus_tag="IYI34_002998"
                     /pseudo
     CDS             2549..3763
                     /gene="aph(2'')-I"
                     /locus_tag="IYI34_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT         1429 a          562 c          719 g         1053 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacaccgac catcgtccag taaagagacg
     2341 caataaattc tatcgaagtt tacgcactgc ctctacaacg attaaaggca tggaagccat
     2401 ccgaggatta tataagaaaa cccgaaaaga aggcactctc ttcgggtttt cggtctgtac
     2461 tgaaatcaag gtattattgg gaatcccagc ttaaatcata gataccgtaa gggattttat
     2521 tctttattta aaactttgca acagaaccga atcattaaaa aaacattata cagagccttg
     2581 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
     2641 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
     2701 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
     2761 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
     2821 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
     2881 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
     2941 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     3001 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     3061 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     3121 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     3181 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     3241 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     3301 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     3361 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     3421 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     3481 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     3541 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     3601 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     3661 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     3721 tgagaaagca aaagaatatc aagatatagt tgaagaatat tat
//
LOCUS       DACJGB010000153         3698 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.161, whole genome shotgun sequence.
ACCESSION   DACJGB010000153 DACJGB010000000
VERSION     DACJGB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.161"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            5..1087
                     /locus_tag="IYI34_002999"
     CDS             5..1087
                     /locus_tag="IYI34_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0560802.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="IYI34_003000"
     CDS             complement(1305..2447)
                     /locus_tag="IYI34_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAR0560803.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="IYI34_003001"
     CDS             complement(2452..2637)
                     /locus_tag="IYI34_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAR0560804.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="IYI34_003002"
     CDS             complement(2991..3236)
                     /locus_tag="IYI34_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0560805.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="IYI34_003003"
     CDS             complement(3230..3631)
                     /locus_tag="IYI34_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAR0560806.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1210 a          672 c          565 g         1251 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gccaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaa
//
LOCUS       DACJGB010000154         3678 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.162, whole genome shotgun sequence.
ACCESSION   DACJGB010000154 DACJGB010000000
VERSION     DACJGB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.162"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            40..792
                     /locus_tag="IYI34_003004"
     CDS             40..792
                     /locus_tag="IYI34_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAR0560807.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            789..1703
                     /gene="pfkB"
                     /locus_tag="IYI34_003005"
     CDS             789..1703
                     /gene="pfkB"
                     /locus_tag="IYI34_003005"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAR0560808.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1721..3652
                     /locus_tag="IYI34_003006"
     CDS             1721..3652
                     /locus_tag="IYI34_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAR0560809.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
BASE COUNT         1184 a          660 c          843 g          991 t
ORIGIN      
        1 aaaaaatatg ctattatttg tattgaggtg atcgtgaaaa tgcttacaga ggaacgcaga
       61 caaaaaatcc tagacttgct tgaacagcag aaaatcgtga aatcacaaga tcttgtaaac
      121 cttttgaatg cttctgaatc aacgataaga cgtgatttac aagagctgga gaatagtggg
      181 tttctagaaa gagttcatgg cggagctaag aaagagcaat tattgaattt tgagcagaat
      241 atgagtgaaa aatcactcaa aaacgttcac gaaaaacagc aaatcgccaa atcggcagct
      301 gaaaccatta atgacgaaga tgtcatttat ttagacgctg gctctactac gttagaaatg
      361 attccttttc tgcaaggaaa acagatcaca gtagtgacaa attccgttca acatgcggca
      421 aatttagttg atttggagct tgctaccatt attttaggtg gaacgataaa gctggcaact
      481 aatgctgttt taggatctaa tgcgatagaa caactaaaaa attttcattt taataaagca
      541 tttatgggaa tgaacggtgc ccatcttgaa caaggattca cgactccgga tccagaagag
      601 gcagctgtta aacgtttagc catgaaaaat tccgaagaga catttgtatt gattgatcat
      661 accaaattca accagttgtc cttcacctct gttgcacctt tggaagcagc cacgatcata
      721 accgagcgtt gtccattaga attcgttaat gaattccgtg aaaagacaac aattaaggag
      781 gcaatacaat gatttacaca gtaacactta atccatcaat tgattatatc gtacgcgtag
      841 atgggttaaa actaggcgat ttgaaccgaa tgaaggaaga cttcaagtta cctggaggaa
      901 aagggatcaa tgtttcccgt attttgaaac ggatcgattc tcaatctaca gcactagggt
      961 ttttaggagg gttcactggc gaatttattt cgaagtggct tgaaaaagaa caaatccagt
     1021 ctttgttcac aaccgtaaaa gaagatacaa gaatcaatat caaactaaaa tcagacactg
     1081 aaactgagat caatggctta ggcccatcta tttcagatga agaaatcgat gagttgaagc
     1141 aagcattagg aacagtagga caaggagata tcgtcgtatt gtcaggaagc gcgccagcta
     1201 gcctgcgaaa aggattttat caagaactga ttgatatcat tcgagaaaaa ggtgcagatt
     1261 tcgtgattga tacaacaggt gatgacctaa aaaatgcact tgttaagaaa ccgttgttga
     1321 tcaaaccaaa caatcacgaa ttagctgagc tttacaacac aaccttccaa tctgtagaag
     1381 aaatcattcc gtatgggaaa aaattactag ctgaaggggc acagaatgtg ctgatctcaa
     1441 tggctggaga cggtgctttg ctcttcacgg aacaaggaac ttataaaacg aatgtattag
     1501 ttcgcccatt gaaaaattca gtaggtgcag gggattcaat gattgctggc tttattgggg
     1561 cttacagccg tacgaaagat ccaatcgaag cctttaaatg gggagttgct tgcggaagcg
     1621 caacagcttt ttctgatgac ttggcagctc gcgaattgat tgatgagttg ctgccagaag
     1681 ttgaaatcac aaaaattgat tagtttcaag gaggataatc atgaatatca aggatttaat
     1741 ggtcaaagaa gcgatgatca tggatttgca agcgactgat aaaaaaggcg caatcgatga
     1801 aatggtccaa aaaatgtttg atgccggccg gatttccgat atcgaaacat ataaagaagg
     1861 aattctagcc cgcgaagcac aaacttctac aggtcttggt gatggaatcg ccatgcctca
     1921 cgcgaaaaac agtgcagtta aagaagcaac agttttattc gcaaaaagcc aaaaaggagt
     1981 agattatgaa gccttagacg gacagccaac ttatctgttt ttcatgatcg cagctccaga
     2041 aggcgcaaac gacacacatt tgcaagcatt agcagcacta tctcgattgc tgattgatgc
     2101 agatttcgta ggaaaattaa aagaagcaaa tacaccagaa gaagtccaag acttgttcca
     2161 aacagctgaa gagcaaaaag aagcagaaga aaaagcagaa caagaaacac aagctgcaga
     2221 agcttctgct catccagaaa gaaaattcgt tgttgccgtt acagcttgtc caacagggat
     2281 cgctcacact tacatggcag aagatgcttt gaagaaaaaa gcgaaagaaa tgggtgtgga
     2341 gatcaaagtc gaaacgaacg gttcagaagg aatcaaaaac cgattgactg ctgaagacat
     2401 cgcccgtgca gaaggtgtaa tcgttgcagc agacaaaaaa gtagagatga atcgctttga
     2461 cggaaaagaa ctgatcaatc gtcctgtcag tgatggtatc cgtaaaccag aagagttgat
     2521 caatctagcc cttagtggta ctgcaccagt attccatggt gatggaaaag aaagcagttc
     2581 agaagaagga aatgcggatg gtactatcgg acaaagaatt tacaaagatt tgatgaacgg
     2641 cgtatcacac atgttacctt tcgttattgg tggaggaatc gctattgcgc tttctttcat
     2701 gatcgaccaa tttattggcg tgccacatga tcaattatca aatctaggga attacaatca
     2761 agctgctagc tggttcaacc aaatcggaca agctgcattt ggtttcatgc tgccggtatt
     2821 agcaggattt attgcttcaa gtatcggtga ccgccctggt ttgatcgttg gatttgctgc
     2881 tggtgcatta gctaatacag gcggtgcagg attcttaggt gcactgattg gtggattctt
     2941 agctggttat gtcatcgtat ttttacgtaa attattcaag aacttaccaa aatcattgga
     3001 tggaattaaa acgatcctct tttatccagt gtttggctta ctgattactg gtttcttgat
     3061 gttgctggtc aatgtcccta tgaaagcaat caatgatgca ttgaacggtt tcttgacaag
     3121 tatgagtggt tcgaatgctg ctttacttgg tgcattatta gctggaatga tggcggcaga
     3181 tttaggcggt ccaatcaaca aagccgctta tgtgtttggt acagcaactt tagctacgac
     3241 tgttgcaact ggcggaagtg tcgtgatggc ttctgttatg gctggaggaa tggtacctcc
     3301 attggcaatt ttcgttgcta cacgtttgtt taagaataaa ttcagcaaaa cagatcaaga
     3361 tgctggtttg acgaacatcg tgatgggact atcgtttgtc acggaaggat cgattccatt
     3421 tgcagcagct gacccgattc gtgccattcc aagttttatt attggttctg ctttaactgg
     3481 cggattagtc ggagctttcg gaatcaaatt actggctcct cacggcggga ttttcgttgt
     3541 attcttactg agtcacccgg tcatgtattt ggtgtttatt gctatcggag cagtcgtatc
     3601 tggtgtgatt tatggtttat tgagaaaagc tcctgcagta gcagaagcat agaaataaaa
     3661 aagaagaggc tgggtttt
//
LOCUS       DACJGB010000155         3640 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.163, whole genome shotgun sequence.
ACCESSION   DACJGB010000155 DACJGB010000000
VERSION     DACJGB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.163"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(136..426)
                     /locus_tag="IYI34_003007"
     CDS             complement(136..426)
                     /locus_tag="IYI34_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560810.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="IYI34_003008"
     CDS             complement(544..804)
                     /locus_tag="IYI34_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560811.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="IYI34_003009"
     CDS             complement(941..1354)
                     /locus_tag="IYI34_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAR0560812.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="IYI34_003010"
     CDS             complement(1810..2295)
                     /locus_tag="IYI34_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560813.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="IYI34_003011"
     CDS             complement(2434..2649)
                     /locus_tag="IYI34_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAR0560814.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..>3640
                     /locus_tag="IYI34_003012"
     CDS             2873..>3640
                     /locus_tag="IYI34_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0560815.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVT"
BASE COUNT         1326 a          579 c          630 g         1105 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg
//
LOCUS       DACJGB010000156         3376 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.164, whole genome shotgun sequence.
ACCESSION   DACJGB010000156 DACJGB010000000
VERSION     DACJGB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.164"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            254..580
                     /locus_tag="IYI34_003013"
     CDS             254..580
                     /locus_tag="IYI34_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560816.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IYI34_003014"
     CDS             complement(697..1041)
                     /locus_tag="IYI34_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560817.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IYI34_003015"
     CDS             complement(1117..2364)
                     /locus_tag="IYI34_003015"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAR0560818.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IYI34_003016"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IYI34_003016"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAR0560819.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          665 c          671 g         1053 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgtagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagt
//
LOCUS       DACJGB010000157         3353 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.165, whole genome shotgun sequence.
ACCESSION   DACJGB010000157 DACJGB010000000
VERSION     DACJGB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.165"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..189
                     /locus_tag="IYI34_003017"
     CDS             <1..189
                     /locus_tag="IYI34_003017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560820.1"
                     /translation="DLEKLTKQRDMKNFNNCVVLIIITELTLFTNIYYIHEAQSSLIE
                     DWAIFMAKEGKEMFQNKG"
     gene            211..477
                     /locus_tag="IYI34_003018"
     CDS             211..477
                     /locus_tag="IYI34_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAR0560821.1"
                     /translation="MDSLPVELQALCWNLIDQNVQKQFPLDYLQIFEFSTEKGNQKLV
                     HRQEEPEERKEYLISPKLRLKSVSQKIWAIDSGEYQTMLLPEEY"
     gene            504..803
                     /locus_tag="IYI34_003019"
     CDS             504..803
                     /locus_tag="IYI34_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAR0560822.1"
                     /translation="MLETAIEVLEKCAQLVTASEEWGYESVTMEKEEIEMGMLPEDVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTDE"
     gene            977..1528
                     /locus_tag="IYI34_003020"
     CDS             977..1528
                     /locus_tag="IYI34_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAR0560823.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFLRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1674..1967
                     /locus_tag="IYI34_003021"
     CDS             1674..1967
                     /locus_tag="IYI34_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560824.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1960..2256
                     /locus_tag="IYI34_003022"
     CDS             1960..2256
                     /locus_tag="IYI34_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560825.1"
                     /translation="MAKLIVLGGLIYGGYWLYQYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHTGYRHYWERID"
     gene            2331..3329
                     /locus_tag="IYI34_003023"
     CDS             2331..3329
                     /locus_tag="IYI34_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAR0560826.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKATIETDTTIETKKSKTQS
                     TEQTTTEMKQSVETSASSITESSSEEVKNTLWDTNKASKLETFVTQWGKTLGQEYKSY
                     NLQNNVSLYGTPLPQAVINGDWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVETGE
                     YLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFSDIVNGQTPQTP
                     VVEESTQQATTTTTQSTSSSGISSWEAAKESVLNNKALWLYDSPDKNVSNTNLISTNE
                     YMKDLMQDENGSYYSINIVTDDDGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK"
BASE COUNT         1261 a          459 c          664 g          969 t
ORIGIN      
        1 gatttagaaa aacttactaa acaaagagat atgaaaaact ttaataattg tgtagttttg
       61 ataattataa ctgaattaac tttatttaca aatatatatt atatccatga agcccaatct
      121 tctttaatag aagattgggc tatttttatg gcaaaggagg gaaaagaaat gtttcaaaat
      181 aaaggataac gttatgtcac aaagggagta atggacagtt tgccggtgga attacaagct
      241 ttatgttgga atctaatcga tcaaaacgtt caaaagcaat ttcctttaga ctatcttcaa
      301 atttttgaat tttctacgga aaaaggaaat caaaagttgg ttcatcgtca agaagaaccg
      361 gaagaaagaa aagagtatct gatttcacct aagcttcgtt tgaaatcagt gtctcaaaaa
      421 atatgggcca ttgattcggg tgagtatcaa acgatgttat taccagaaga atactagtat
      481 ctaaaagaaa ggaacgaaag gacttgttag aaacggctat cgaagtactg gaaaagtgtg
      541 cacagctggt cactgcttct gaagagtggg gctatgaatc cgttacgatg gaaaaagagg
      601 aaattgaaat gggtatgctt cctgaagatg ttcatctacc acgtttagtg atgacacatc
      661 tttatattta ttgcgcacca gaagatggaa aagactatgt agtttatttc attacagata
      721 ttaccagcca gcgagaattt gttcgtgggt tactggtaga aggacgattg gtttggagtc
      781 agattggagg aacggatgaa taagatcgat tatttgagaa tgattgcgaa atgtttagaa
      841 gatatcctta ccgcgattca tcaaattctg aagaaaaaag gagaaagaat atgaaatatt
      901 ttcaactagt attgacactt ataacggcaa tggtaattga agtgcttgaa caaaaaatag
      961 cataggagaa aaagagatga ttgaaaaaga attagtgacc acacaaacag aagtttggcg
     1021 agatcctcat catacccatc gctatgtttt caaacgacaa tgggcgatca aaggaaaaga
     1081 agcggaagat aaattagcag tagtcattac tattcgtcca acagatacag aaccatttac
     1141 gaatgatttg agtatgttat taatcgaaaa gaatatccga caattaggat ttactggttt
     1201 tattgctgtg aatctttttt cgtacacaaa agcgaaaagt cctcgggtat ttcttcgagg
     1261 aaatgatgaa caatctttgg aaatattaac gactgttttt acagaaaaga aaatcaatac
     1321 cattattttt gcttgtggtt cgattacagc gacgagtcaa gtggcttatg aacaagcaaa
     1381 gactatttac gatcaattga gtgcgaaaca aaagaagatg accaaagtat tgataaatgc
     1441 agaaggtaag ttatcccacc cactaagtat tcatgttcgt aatgaatggc aattggcaga
     1501 tagtcaatta ttatttcaag aaaaataaca ttttattttc tcatgttact tataagtagt
     1561 tttacatgct ttttgacttt aaaaatacat gtgatgatac atgttatatg aattatcaat
     1621 gtacccagta atatgctatt ttcattatgt aaaaaataac agggggtgtg tagatggaga
     1681 ttgatgaaac ttgtttgagt aactcttata aaagtatgaa tgactttttt gaggaaatgg
     1741 aactggattc agaattgctc tatcaatttt atcttgcttt tcgagggcaa cagatttctt
     1801 ttccaatgaa aatgtacgat cgtgagttag ttagaaaaag aatcgaaaac atgatagaac
     1861 aggaaaaaac tgtagatatc cgtcaattga cagaagttta tggtttctct gcaagatgga
     1921 ttcaaaaagt aattaaacaa aaagaaagga gacgagtaca tggctaagct gattgtatta
     1981 ggtggtttaa tttacggtgg atattggtta tatcaatata tgcttgcgca tccgaatata
     2041 gataaaggaa aagtaattaa gacagttggg gtaatcatag ttggactcat agctctaact
     2101 aagaaaaata atactaatag cccaaaaata atgagaactt atcgctgtag aggatgtgga
     2161 agggaaattt ctactagttt taatccatct gagagtcctc aaggaagaga ccgctgccat
     2221 acgggctatc gacattattg ggaaagaatc gattaaaaga ttaccattta gatcaattaa
     2281 ttgtctgata gaataagtac gtgagggaga aattgaaagg gggaaatata atgaaaaaat
     2341 ggctgttatt actaatagga atgggaattt tattcactgg ttgtacatct gagaatacga
     2401 ggactaaagc gactatagaa actgatacaa caattgagac taaaaaaagt aaaacgcaat
     2461 caactgaaca aactacaaca gaaatgaagc aatctgtgga aacaagtgca agttcaatca
     2521 ctgaatcttc ttctgaagag gttaagaata ctttgtggga tacgaataaa gcttccaaat
     2581 tagaaacttt tgtcacacaa tggggaaaga cattaggtca agaatataaa agctacaatc
     2641 ttcagaataa tgtaagtctg tatggcactc ctttaccaca agccgtgatt aatggcgatt
     2701 ggaaaatggc aatcaatgaa gcaccagtta ccgtacaatg gtcagaagat ggaactggtc
     2761 atgcagattt caatttagta gcagtttatt ctgatgtaga aactggagaa tatttagggc
     2821 agcatgttta ctttttcggt tttcaaaatg gacaacctaa agtttattta acgcaacaaa
     2881 atcaaggtaa tgaaaataat tatctgtact ttaacgaaac tcaaaatcaa cagttaaaac
     2941 aaggtttcag tgatattgtg aatggacaaa ctcctcaaac accagtagta gaagaatcta
     3001 ctcaacaagc aacaactaca acgacacaat ctacaagtag ttcaggaatt tcaagttggg
     3061 aagctgctaa agaatctgtg ctaaataata aagctttatg gttgtatgat tctcctgata
     3121 aaaatgtatc aaatactaat ttaattagca caaatgaata tatgaaagat ctaatgcaag
     3181 atgaaaatgg aagctactat tcaattaata ttgtaacgga tgatgatgga aacacgacca
     3241 tggctggtgg aaaacaattt cgtgtttata cagatggaag aatttctcaa cgtattggaa
     3301 tgactaacaa ttttcaaatg attaaataat tgaaaatatt atttatcaaa gaa
//
LOCUS       DACJGB010000158         3219 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.166, whole genome shotgun sequence.
ACCESSION   DACJGB010000158 DACJGB010000000
VERSION     DACJGB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.166"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            50..1438
                     /locus_tag="IYI34_003024"
     CDS             50..1438
                     /locus_tag="IYI34_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HAR0560827.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>3219
                     /locus_tag="IYI34_003025"
     CDS             1776..>3219
                     /locus_tag="IYI34_003025"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAR0560828.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          583 g          948 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgaaca
     2581 tgattttgca tggggttccc attgccaacc aacacttgca taatgcggat accttggatc
     2641 aagactggcc aacagaagag ccaacgaact tcgatggggt gctcatgaac ccaccgtatt
     2701 ctgcaaaatg gtcggctgat aaaggcttct tagatgaccc acgcttctct gcctatggcg
     2761 tattagcccc taaatcaaaa gcggactttg ctttcttatt acatggctac tatcatttga
     2821 aagatacggg cgtcatggcc attgttttgc cacatggggt gctctttaga gggggcgcag
     2881 aagggaagat acgcaaagcc ttgttagaaa atggtgccat tgacaccgtc attggcttac
     2941 ctgctaacat cttctttaat acgtccattc caacgaccgt aattatcttg aagaaagacc
     3001 gtgcgaaaaa agatgtgctc tttattgatg cgtcacaaga ttttgaaaaa ataaaaacgc
     3061 aaaatacgct acgagattac catattgacg ctattttaga ggcctataaa acccgtacaa
     3121 ttaaagacaa gtatgcttat gtcgcagaat ttgatgaaat tgtggagaat gattacaact
     3181 taaacattcc ccgttacgtg gatacctttg aagaagaag
//
LOCUS       DACJGB010000159         3064 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.167, whole genome shotgun sequence.
ACCESSION   DACJGB010000159 DACJGB010000000
VERSION     DACJGB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.167"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            581..1540
                     /locus_tag="IYI34_003026"
     CDS             581..1540
                     /locus_tag="IYI34_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR0560829.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="IYI34_003027"
     CDS             complement(1527..2132)
                     /locus_tag="IYI34_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560830.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
     gene            complement(2372..>3064)
                     /gene="tnpB"
                     /locus_tag="IYI34_003028"
     CDS             complement(2372..>3064)
                     /gene="tnpB"
                     /locus_tag="IYI34_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAR0560831.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          998 a          509 c          478 g         1079 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc ttgggaagag
     2281 agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac caagctatcc
     2341 ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag tgtagcctta
     2401 gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga gtatgacaaa
     2461 tagggcaagt ccattttcga atttcgagag atttcttgcc gtctttatgt ccacaattcg
     2521 aacagatttg gctagatgga aaccacttat ctactttgat gattttccga ccgtaccagt
     2581 cagccttgta ttgtaattta gacacaaaac tagaccaaga aacatccgaa atactttttg
     2641 ctagtttatg attacgcaac atgccttttg tgtttaagtc ttcaatacag atgatatcgt
     2701 gatttttgat aatttctgta ctcaacttat tcagaaaatc agtacgttga ttcattactt
     2761 tttcatgcaa tcgtgctact ttacgttttt gtgtttgata atttctagct tcaaacagat
     2821 tgataccttt ttgtttagct aataaggcac gtcttgacaa cttacgttgt tcacgtttga
     2881 gtttcttttc cattttggac gtaaatttat tattatcaaa tttttgaccg gtagaaagaa
     2941 ttgcaaagtc tataatacct aggtcaatac caacagcaga attagattta ggtagttcgc
     3001 gaacttcttc tttgcataac aaagaaatat aatatttacc gctagaatga cgtgatattg
     3061 tagc
//
LOCUS       DACJGB010000160         3035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.168, whole genome shotgun sequence.
ACCESSION   DACJGB010000160 DACJGB010000000
VERSION     DACJGB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.168"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(28..963)
                     /gene="murB"
                     /locus_tag="IYI34_003029"
     CDS             complement(28..963)
                     /gene="murB"
                     /locus_tag="IYI34_003029"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAR0560832.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1036..1956)
                     /locus_tag="IYI34_003030"
     CDS             complement(1036..1956)
                     /locus_tag="IYI34_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAR0560833.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2069..2821
                     /gene="xth"
                     /locus_tag="IYI34_003031"
     CDS             2069..2821
                     /gene="xth"
                     /locus_tag="IYI34_003031"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAR0560834.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          854 a          611 c          525 g         1045 t
ORIGIN      
        1 tttacttatt ttaagttcca ttacttatta attggctaaa cttctgcttg tttctgatat
       61 tgcttgtttt tctccaatga tccgcacttc tgtttctaaa gacacatcaa atttttcttt
      121 gattacttct tggatgtgcg caatcaattc aatataatct gtcgctgtcg catgatcgat
      181 attgacgatg aatccagcat gtttttcaga gacttgtgca ccgccccatt tcaggccttg
      241 caatcctgct tgttggatca gctgacctgt atagtgtcct actgggcgct taaagacact
      301 tccgcatgaa gggtattcta atggctgttt tgattgacgc aattcggtca actcatccat
      361 tcttgctttg attgcttcat aatcccctgt ttgcaaactg aatcgagctt ccaatacgat
      421 agcacgtaat tcttggactt tactgtgacg ataagaaaat accatttcgt cttttgtcaa
      481 agttttcaat gttccatctt caagcaaaag atccacttca gcaaaaacat ctttgatctc
      541 tccgccatac gcacctgcat tcatataaac cgctccaccg acacttcccg ggatgccgca
      601 agcaaattca aatccagtca atgattctgc caaagctgca taagtcgtat caatcagtga
      661 ggcacctgct tccgcaatga ccatcgttcc tttgacatga atctgtttca tttcagtcag
      721 catgatgact acaccgcgaa tcccgccatc ttgaacgatc agattgcttg catttcctaa
      781 taccatccaa ggtatatcat ggattcggca ataatcgatg atttgcttta cttcttcttg
      841 ttttttcggg aatgcaagga catcagctgg tccacctgtt ttcgtaaacg tatagttcat
      901 caatggttca tcaaacaata tagtaatcgt ggctaagctt tcaactattt cttctttatt
      961 cactgaggat tttcctttct tttctcaaac gtcctcatta ttctatcacg cttaatacaa
     1021 tcttgccagt cttgttcaaa gttgatgacg gtagtgatct ttaagcactt cctcacataa
     1081 ttcatgactt cttacaactt ctttttgttt aagcaattct gtttccgatt tacctaaacg
     1141 gatctgagaa aagaaggcct ctaccattgg ctcaaacccg cgtttgacta gcgtgtttgt
     1201 ccagtcgcca aattctttca ctttttttcc atcagcatct tgaatgacaa gctgggtcag
     1261 atcgtttact tcataagtac catttttact tgttacttga tatttttcta tattagcgcc
     1321 acttgctaaa tccatcgtta aaatagccgt ttgattttgt gtttctaatt gtaaaatggc
     1381 atattccaaa aattcctttg tttctcttat ctggtatttt gttctcaaaa tgggttcatc
     1441 taataaatac acagctgtat caataagatg taaaaacaaa tcataaagga cgaactctgt
     1501 cgtttctttt gccgctattc gatttttttg caggtggatc aaccgcttat ctgggatacg
     1561 tttcaattct tctaccatag gagcaaagcg ccggttgaaa ccgatcatca aaattatttg
     1621 gttcttatca gctaattctt gcaattcacg aacttcattt agatggacac tcagtggttt
     1681 gtcaatataa acatggatac cattttctag acattttttg gctaattcat aatgtacttt
     1741 tgttgctgcg tggatcatac atgcttcgat tttttcttct aaaagctcat cgatcgtttc
     1801 atatagatgt tggaacccgt actctttttg aattctttct tttgtttctt tatttcttgt
     1861 tgcaaaataa aaatcaagtg ttccttgttt tttcgcataa gttggtaaat atgctttttc
     1921 tgcgatattc cctacaccga ttacaccaat cttcatctac tttcctcctg gatcatttag
     1981 ttactttcat cttacaccat tttttactaa aatgaggtac aatagtatcg caaaggtaag
     2041 gaaattaatt cgaaggagtg tgcttttatt gaaatttatc tcttggaacg taaacgggct
     2101 tcgtgccatc gtaaataaaa attttttaga agtttttcat gagttagacg ccgatttttt
     2161 ctgtctgcag gaaacgaaac tccaagcagg acaaattgat ttagatcttc caggttatta
     2221 tcaatattgg aattacgcgg aacgaaaagg ctattccggc acagcgattt ttgctaaaaa
     2281 accagcattg aatgccactt acggaatggg tatcgatatc catgatacag aaggccgtct
     2341 gattactttg gaatattccg atttctttct tgtaacctgt tacacgccaa attctcaaag
     2401 cgaattaaaa cgtttagatt accgattaga atgggaagaa gccttttata attatttgga
     2461 aaatctaaaa aaacaaaaac cagtcatcgt gtgtggggat ctaaatgtcg ctcatcaaaa
     2521 gattgatttg aaaaactgga aaacgaatca aaaaaatgct ggattcactc cagaagaaag
     2581 agcggctctt tctcgactgt tggataacgg gtttatcgat acattccgtt atttttatcc
     2641 aacccaggaa ggtgtctact cttggtggaa ctaccgattc aattcaagga aaaacaatgc
     2701 tggttggcgg atcgattact ttttgacgag caaagattta gagccaggac tagctgatgc
     2761 aaagatccat acggacatta tgggcagtga tcattgtcca gtagaattag atttgaaata
     2821 aagaggttgt gaaaaagtcg tttagtttcg agtattaaga aaaattttgt gaaaatggct
     2881 ttccacattt tgcataaatt tggcataata ccgagaaact ggcttttgaa cactgtttat
     2941 cgagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaaa caaaaatagt
     3001 tcctcatatt tttcgtttgg ttgggcttat taccg
//
LOCUS       DACJGB010000161         2952 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.170, whole genome shotgun sequence.
ACCESSION   DACJGB010000161 DACJGB010000000
VERSION     DACJGB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.170"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            89..406
                     /locus_tag="IYI34_003032"
     CDS             89..406
                     /locus_tag="IYI34_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAR0560835.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="IYI34_003033"
     CDS             complement(497..862)
                     /locus_tag="IYI34_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560836.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="IYI34_003034"
     CDS             1070..1552
                     /locus_tag="IYI34_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560837.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /gene="pstS"
                     /locus_tag="IYI34_003035"
     CDS             1749..2639
                     /gene="pstS"
                     /locus_tag="IYI34_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAR0560838.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DACJGB010000162         2947 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.171, whole genome shotgun sequence.
ACCESSION   DACJGB010000162 DACJGB010000000
VERSION     DACJGB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.171"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(449..865)
                     /locus_tag="IYI34_003036"
     CDS             complement(449..865)
                     /locus_tag="IYI34_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560839.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IYI34_003037"
     CDS             complement(867..1148)
                     /locus_tag="IYI34_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560840.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IYI34_003038"
     CDS             1948..2922
                     /locus_tag="IYI34_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAR0560841.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACJGB010000163         2857 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.172, whole genome shotgun sequence.
ACCESSION   DACJGB010000163 DACJGB010000000
VERSION     DACJGB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.172"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            562..843
                     /locus_tag="IYI34_003039"
     CDS             562..843
                     /locus_tag="IYI34_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560842.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IYI34_003040"
     CDS             845..1261
                     /locus_tag="IYI34_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560843.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IYI34_003041"
     CDS             complement(1422..1688)
                     /locus_tag="IYI34_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560844.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IYI34_003042"
     CDS             complement(1646..2620)
                     /locus_tag="IYI34_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAR0560845.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACJGB010000164         2796 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.173, whole genome shotgun sequence.
ACCESSION   DACJGB010000164 DACJGB010000000
VERSION     DACJGB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.173"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IYI34_003043"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IYI34_003043"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAR0560846.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1086 a          435 c          568 g          707 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaaa aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattgg
//
LOCUS       DACJGB010000165         2736 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.174, whole genome shotgun sequence.
ACCESSION   DACJGB010000165 DACJGB010000000
VERSION     DACJGB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.174"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            35..769
                     /locus_tag="IYI34_003044"
     CDS             35..769
                     /locus_tag="IYI34_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0560847.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            802..1710
                     /locus_tag="IYI34_003045"
     CDS             802..1710
                     /locus_tag="IYI34_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAR0560848.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1707..2249
                     /gene="sat4"
                     /locus_tag="IYI34_003046"
     CDS             1707..2249
                     /gene="sat4"
                     /locus_tag="IYI34_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAR0560849.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2342..>2736
                     /locus_tag="IYI34_003047"
     CDS             2342..>2736
                     /locus_tag="IYI34_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAR0560850.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCP"
BASE COUNT          951 a          406 c          634 g          745 t
ORIGIN      
        1 gagataaata actttttaaa cgaggagagt tcagatgaaa gaaaacaaat atgatgataa
       61 tatatttttt caaaaataca gtcaaatgag tcgctcgcag aaaggactgg ctggtgcggg
      121 agaatgggag actttgaaaa agatgctacc tgattttaag ggtaagcgtg tgcttgattt
      181 aggatgcggc tatggatggc actgtatata tgcgatggaa aacggtgctt cctctgtagt
      241 aggtgttgat atttctcata aaatgctcga agtagcaaaa ggaaaaaccc attttccaca
      301 gattgaatat gaatgctgtg ccatagaaga tgtggatttc ccagaggaga gctttgatgt
      361 aatactaagt tcgcttgcgt ttcattatgt agcagactat gagaatttaa taaaaaagat
      421 atataggatg ctgaaggctg gtggcaattt agtttttaca gttgaacatc ctgtttttac
      481 tgctcatgga acacaagact ggtattataa cgaaaaagga gaaatactgc atttcccggt
      541 ggacaattat tattatgagg gcaaacggac agctatgttt ttggaagaaa aggttacaaa
      601 atatcataga acactgacca catatctaaa tacactgctt tcaaatagtt ttataataaa
      661 tcagattgtg gagccacagc cgccagagaa catgatggat attccgggga tggcggatga
      721 aatgcgacgc ccaatgatgc tgattgtatc ggcaaaaaag aagatgtaat aatatagaaa
      781 aaataaacga ggagtatgta aatgagatca gaaaaagaaa tgatggattt agtactttct
      841 ttagcagaac aggatgaacg tattcgaatt gtgacccttg aggggtcacg cgcaaatatt
      901 aatataccta aagatgaatt tcaggattat gatattacat attttgtaag tgatatagaa
      961 ccgtttatat ctaatgatga ctggcttaat caatttggga atataataat gatgcaaaag
     1021 ccggaggata tggaattatt cccacctgaa gaaaagggat tttcctatct tatgctattt
     1081 gatgattaca ataaaattga tcttacctta ttgcccttgg aagagttaga taattaccta
     1141 aagggcgata aattaataaa ggttctaatt gataaagatt gtagaattaa aagggacata
     1201 gttccgactg atatagatta tcatgtaaga aagccaagcg caagggagta tgatgattgc
     1261 tgcaatgaat tttggaatgt aacaccttat gttattaaag gattgtgccg taaggaaatt
     1321 ttatttgcta ttgatcattt taatcagatt gttcgccatg agctgctgag aatgatatca
     1381 tggaaggtcg gcatcgaaac aggctttaaa ttaagtgtag gcaagaacta taagtttatt
     1441 gaaaggtata tatccgagga tttgtgggag aaacttttgt ccacctaccg gatggattcc
     1501 tatgaaaaca tatgggaagc attatttcta tgccatcaat tgttcagggc ggtatccggt
     1561 gaggtggcgg aaaggcttca ttatgcctat ccggagtatg ataggaatat aacaaaatat
     1621 accagggaca tgtataaaaa atacactggt aaaaccggct gcctggatag cacatatgcc
     1681 gctgatatag aagagaggcg ggaacagtga ttacagaaat gaaagcaggg cacctgaaag
     1741 atatcgataa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     1801 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     1861 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     1921 agataatata tctttactac caagacgata aatgcgtcgg aaaagttaaa ctgcgaaaaa
     1981 attggaaccg gtacgcttat atagaagata tcgccgtatg taaggatttc agggggcaag
     2041 gcataggcag cgcgcttatc aatatatcta tagaatgggc aaagcataaa aacttgcatg
     2101 gactaatgct tgaaacccag gacaataacc ttatagcttg taaattctat cataattgtg
     2161 gtttcaaaat cggctccgtc gatactatgt tatacgccaa ctttgaaaac aactttgaaa
     2221 aagctgtttt ctggtattta aggttttaga atgcaaggaa cagtgaattg gagttcgtct
     2281 tgttataatt agcttcttgg ggtatcttta aatactgtag aaaagaggaa ggaaataata
     2341 aatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat accgctgcgt
     2401 aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa
     2461 cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg tggaacggga
     2521 aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga
     2581 acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc tttgctcgga
     2641 agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag
     2701 gctctttcac tccatcgaca tatcggattg tcccta
//
LOCUS       DACJGB010000166         2447 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.176, whole genome shotgun sequence.
ACCESSION   DACJGB010000166 DACJGB010000000
VERSION     DACJGB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.176"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            415..652
                     /locus_tag="IYI34_003048"
                     /pseudo
     CDS             415..652
                     /locus_tag="IYI34_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(796..1572)
                     /locus_tag="IYI34_003049"
     CDS             complement(796..1572)
                     /locus_tag="IYI34_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAR0560851.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            1695..2102
                     /locus_tag="IYI34_003050"
     CDS             1695..2102
                     /locus_tag="IYI34_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAR0560852.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT          845 a          392 c          375 g          835 t
ORIGIN      
        1 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
       61 attttgccgt ttgttgagct aataccgaag gtcagctgat tgaacaccgt ttaagaaaga
      121 ggtcgtgagc ctgtcattta actctgagtc acaagtaaca atttcaaaaa acagcttctc
      181 atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt
      241 acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttaatgagga
      301 cagtcggaac aggtttgtca tttttatatt taaatggcac aaatttttct aattatttgt
      361 catttagcgc tgaaaatttt tgctatttat ctaaaaaagt gataactact taagatgaag
      421 cagaatattt acgccatacg gcatacaacc gcgaaaaata taaaaaaaag aaaacgattg
      481 aacgcgtttt tgcagtcgca aaggaaaagc atggaatgcg ctacatcaag tatcgagact
      541 tggaaaacaa tcaggtgtat acgctgattg tttttgctgc aatgaatctg aaaaagttgt
      601 ccacttggca gttggaacat atgtcttcat atcttttatt atacaaaaat agcaaaagca
      661 ccagtctaca ctcttagtaa agaggaaact gatccttttg tctacattct taaagtactt
      721 aaactaagta ctttatttta ctctttagga ttgatacagt agcatctatc cctttattaa
      781 taatctttta tttttttaaa taaagatgtt gtctccatct tcaggcacta aaactttttc
      841 ggaaattcct ttttctttat tgaaagactt taattcttct cttgacagtc cccaatggtt
      901 tacagcttcc atatgaacgg caataattgt ggaatctggt attgctttat gcaattcata
      961 taaatcgtct ttccccataa taagagattc tccaactaaa aaatgattat ctccagcatt
     1021 tgctactatg atttctggat tatactgatc aatagtttgt ttaacagctt cataccaaac
     1081 tgtatctcct gtaaagtata gtgtcttctc agtcgaatgt ttgaaaacaa taccgcaaac
     1141 attacctaca taattcaaga tttctcctct gccatgtttg ccttttgttt taaccaattg
     1201 tatagattca aaaactgtgt cttctcgtag tacctccaca tttttaaaac catatagtcg
     1261 aatttcattc gcatcatttt catcttgcac aaataatttt atttctttag gtaggacctc
     1321 tttcgctata tcgtcccaat gatctagatg taaatgtgtt acaataacag catcaatacc
     1381 gttgataata tcttcgactg gcattggcaa atcaactaga ggattcattt gatcttttct
     1441 tggtgatgga aaaggacctt tcgaaataaa tggtggaagt gttccttttt ccgctaacat
     1501 tggatcaatt aaaaattttt tacctccata gtctaagata gctgttgcat tacgaatgtg
     1561 atgtatattc ataaacgttg tctccttttt gttgatatga aatattttta gtataattca
     1621 attccataca ataaataaac cgaacaaaaa aagtctttta ctatttttac ttaatttatg
     1681 aaaggttgtt taccatgtta gaccaaacag atataaatat cattgaggaa ctatcaaaaa
     1741 atagtcgaat cacaatgaaa gaacttggag aaaaagtaca tttgactgga cccgctgtct
     1801 cggcgagagt gacaaaacta gaggaaagtg gagttattga aaattataca gtaaaattaa
     1861 atatggaaaa gctagggttt tcgattcacg cttttttaat tataattacg caaaatttaa
     1921 atcataaacc ctatttatca tttattaaaa atcatgaaaa attcttgatt agaaattata
     1981 agattagtgg agggggctgt tatcttttgg aatgtagatt tacttcaaaa aaatcaatga
     2041 acagcttttt ggaacagctt aatgaatatg cgaattataa attatctatt gttatagatt
     2101 aataataaaa ctagcagatg gtaaaaatac taatatgcga gacacacatt caaccataac
     2161 tatataactt atcaatttaa aactaaactg atggaaacaa gtaaggcaaa aacatttata
     2221 ttttgcccta cttatttttt taattaaatt cattgatgat tcatatttag aaaattctat
     2281 gaaggccaaa tgagtatcct gtcacttttt tagataaata gcaaaaattt tcagcgttaa
     2341 atgacaaata attagaaata tttgtgccat ttaaatataa aaatgacacc aagtatatca
     2401 tgaacgcaca aaaagaccaa cagggtatac cctgttggtc taaataa
//
LOCUS       DACJGB010000167         2417 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.177, whole genome shotgun sequence.
ACCESSION   DACJGB010000167 DACJGB010000000
VERSION     DACJGB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.177"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            152..412
                     /locus_tag="IYI34_003051"
                     /pseudo
     CDS             152..412
                     /locus_tag="IYI34_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
     gene            575..1240
                     /locus_tag="IYI34_003052"
                     /pseudo
     CDS             575..1240
                     /locus_tag="IYI34_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            1276..1521
                     /locus_tag="IYI34_003053"
     CDS             1276..1521
                     /locus_tag="IYI34_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAR0560853.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1624..>2417
                     /locus_tag="IYI34_003054"
     CDS             1624..>2417
                     /locus_tag="IYI34_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAR0560854.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYC
                     INEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTEKLY"
BASE COUNT          853 a          352 c          475 g          737 t
ORIGIN      
        1 taaatttgga aagttacacg ttactaaagg gaatggagat aaattattag atatactact
       61 gacagcttcc aagaagctaa agaggtccct agcgcctacg gggaatttgt atcgataagg
      121 ggtacaaatt cccactaagc gctcgggacc ccttgtagga aaatgtccta agtgtggcaa
      181 caatattgta ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaagtttac
      241 tttagctgaa cattttagaa agaaaaaact aaccaaaacg aatgtaaaag aattactgga
      301 gggaaaagaa accctggtaa aaggaatcaa aaacaaagag aaaaagccct acaatgccgt
      361 tgtaaaaatt ggggaaaagg gatatattga ttttatctct ttttcaaaat aaacatgggg
      421 ctgacccaaa agtagctcct atatgaaaaa tagaggtctt tccatttaga tagtagtgga
      481 aagacctcta tttatctttt gaaagtgaat acaaaaataa agcacactct gctataatat
      541 aggtgattaa tccatttgag agaggtgctt tatcatgtat caaaattata ccataaacca
      601 actttgtttg cctatagatc ttgaaattaa attagaagaa aatgattttg ctcatgccat
      661 tgtgcagttt gttgactcta ttcccgatga agtctttctt ccttattatc aatctatggg
      721 tagaccccaa tatcatcctc gtatgctact ttccattatt ctttgtgcct atattcaagg
      781 ggtttattcg ggccgccaaa tacaaaatat gctgatagat agtattcgga tgcgctactt
      841 atctcaagag caattcccta actttcgtac tattaatcgt tttcgcgttc atccgattat
      901 gaacgatatt ctagatcatt catttgttca atttcgtgag ctactggttc aaagtggctt
      961 aatcagtggc agtgctctat acattgatgg tacaaaaatt gaagcggatg cgaataaata
     1021 ctctttcgtt tggaaaaaaa gtattttaaa gtataaggat agccttgatc aaaaagccct
     1081 tgaaaactac caacaaatgg tggaaacaaa aatcttacct gagttaatag atgatttaaa
     1141 ggatgaactt tctatagaag agattgaaag gattcgtcaa tctttggaag aaaaggaaaa
     1201 tcaattaatt gaagcgattg accaaaccga aacagtagaa gggggctgac ccattattaa
     1261 taaggagtgg acaacatgat tgatgtaaat gagctattgg aagaagctat tagagaaact
     1321 gaaaatctaa atgatggcga agtttttctt gttaaagatt tattcaaggg atatgtgggg
     1381 aataggatac ccagaaagga tcgacttctg cttggaacgt tatttttaaa ccatgttagc
     1441 aaaatgaatg gtaatttaaa ggctattgaa aagacttcat ctaatcagca gaggtataaa
     1501 aagacaattg ataagtttta actatctttt gtgaaattaa aaaaattata aatatgttga
     1561 gtagaagcaa aagatttggc ttggaatttt tacagcagtg aatcgataaa aggagcatgc
     1621 ttgatggtag ataatattat taaatcagta gcagagaaat tatcctctct gtcttatata
     1681 gaaggtattg ttttaggtgg ttcacgtgca aggggcaccc atacagagga ttcggatata
     1741 gatatcggca tctattacaa ttcagaatca tttgacataa atactattaa tcaattcgct
     1801 acaaagctgg atgatgagca tagaaataac cttgttgtac ctcccggagc atggggtgat
     1861 tggattaatg gcggcggatg gttagtcata aacgggtatc atgtggattt aattttacgt
     1921 gatattaaac gtgtggaaca aataatgaaa gatacagagc acggaattgt tactgccaat
     1981 tatcagactg ggcatcccca tggttatatt agtgcaatgt atcgaggaga attagcgatt
     2041 agcaaaatac tatatgctaa gaatgaaagc ttatgcgaat taaaaaaaca ggcagaaact
     2101 tatcccaatg ctttgcagaa aagtttagtt aactttttta tgtttgaagc agggttctct
     2161 ttaatgtttg taaaagcaaa ttcgggaaca gacgataaat attatattgc gggtcatgtt
     2221 tttcgtatag tttcatgttt aaatcaagtg ttatttgcat gtaataatgc ttattgtatc
     2281 aacgaaaaga aagctataaa actgcttgaa acttttgaac ataaacctga aaaatatacc
     2341 gagaaggtaa atcatatttt tgaagtactc ggtatctcac tttttgaatg ctacgacatg
     2401 accgagaagc tttataa
//
LOCUS       DACJGB010000168         2398 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.178, whole genome shotgun sequence.
ACCESSION   DACJGB010000168 DACJGB010000000
VERSION     DACJGB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.178"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            207..2069
                     /locus_tag="IYI34_003055"
     CDS             207..2069
                     /locus_tag="IYI34_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0560855.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IYI34_003056"
     CDS             2069..>2398
                     /locus_tag="IYI34_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAR0560856.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACJGB010000169         2288 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.179, whole genome shotgun sequence.
ACCESSION   DACJGB010000169 DACJGB010000000
VERSION     DACJGB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.179"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..607
                     /locus_tag="IYI34_003057"
     CDS             <1..607
                     /locus_tag="IYI34_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAR0560857.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSASAISSIVIPPK"
     gene            620..1528
                     /locus_tag="IYI34_003058"
     CDS             620..1528
                     /locus_tag="IYI34_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HAR0560858.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            1528..2148
                     /locus_tag="IYI34_003059"
     CDS             1528..2148
                     /locus_tag="IYI34_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560859.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          789 a          339 c          537 g          623 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
      541 tggaaagtta gatcgccagt attatagtgc aagtgcaatt tcttccattg tcataccacc
      601 aaaataggga ggtgatttaa tggcaaagtg gaatgtcata ttaagtacaa cagaacccta
      661 taattacgtg gggatgattc aagttcgaca aggcaataag aatacagagg ttatggaagc
      721 gactatagtt gaaaatggtc ttccctacga tttatcagaa tgtaaggtat attttgaatc
      781 agttgtaggt gggaaatatc cagtccaatt agaaacaaaa attgtggatg ctaaaaaagg
      841 gaaaattaac tatatttttg ataaatattc catgcagtgt ttacatcgac aaacagccaa
      901 tttcattata tttaaaggag aagacttgat tggaacaact caagacttct cttattttgt
      961 cattaatgct gtttcaaaaa cagaaggaga aatgggttct tattggcaat caatcgaaga
     1021 tttaattgcg gatatgacgg actttattaa tgaaaataaa ggcgatttta cggactggat
     1081 gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa caggaacaca gtttccaaga
     1141 ttggagagaa ggacaagaat cagactattt ggcatggttt gaatcaatca aggatatttt
     1201 aaagacgatt gatccaggtg gagtcatgtt agccgaacta acggatgcgc gagtagatat
     1261 tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg gcagatatgg actatttata
     1321 tcagaaattg cgagcaacgc ttttcacgat tgaatacggg gagattgaag tgactgatat
     1381 tttgcaggat gatctctttt cagataatca tgaagtcgaa aaagttggaa ctgtagcatt
     1441 cccgattgaa gaaggagcct tgatcatcgc aaccgttgat gatccaaagc aaaatgtgtt
     1501 cactcttgag aaagttgggg tgatctaatg gctaaaacta aacgaatgat ggaaacggat
     1561 gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac atgcttccgc agttcttggg
     1621 ttagaagaaa taatagcagg agaagcaaca gttttatctg ttaatggaaa aattggagcc
     1681 gtcgtcatta ctaaagaaga tttgggatta gagaatgttc tcacagaatt accctatgca
     1741 agtgaagaag atgacggcat tatcacggca gaaatgtatc aaaaaatttt aaacagtgga
     1801 gaaggtgact acgtgttgcc agttgccact atcgaccgtt tgggtggcat aaaagttggt
     1861 gaattattga cgattgatga gacagggaaa gtctctgcag tcagacaatc tgatgtcaat
     1921 ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa attatactgc tggagaaaac
     1981 atcactattg atgaagatgg aacaattagt tctacaggtg gtagtgggac tggtggtgtc
     2041 aatcagtcat atgtagatca aaaattccaa gaagctgtaa atcaagcaga aaattacaca
     2101 aacgaaagaa ttccaaattt tacttttgaa aaaattgggg aggtatagag aatgacagat
     2161 atcgtaaaaa taaaacaaag caatgtacag gtttatccac aaactcattg gaatgctata
     2221 gaaggcaaac caacaacggt gaagggagat aaaggcgatc ctggccaagc agcaacaatt
     2281 accatagg
//
LOCUS       DACJGB010000170         2262 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.180, whole genome shotgun sequence.
ACCESSION   DACJGB010000170 DACJGB010000000
VERSION     DACJGB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.180"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(151..2148)
                     /locus_tag="IYI34_003060"
     CDS             complement(151..2148)
                     /locus_tag="IYI34_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAR0560860.1"
                     /translation="MIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKA
                     LVTNKANQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHL
                     KEATKRLSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVY
                     IKNSGVTDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADE
                     AEKYLAKGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGS
                     NLMLSMNAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHE
                     QGSKEITENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKS
                     SVFNRSGQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRD
                     SFKEFGLGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYT
                     PIQLAQYASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMA
                     ILREGFHQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGP
                     TDNPEIAISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          576 a          505 c          411 g          770 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aa
//
LOCUS       DACJGB010000171         2211 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.181, whole genome shotgun sequence.
ACCESSION   DACJGB010000171 DACJGB010000000
VERSION     DACJGB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.181"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IYI34_003061"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IYI34_003061"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAR0560861.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          588 a          480 c          405 g          738 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg cggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtgtat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACJGB010000172         2056 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.183, whole genome shotgun sequence.
ACCESSION   DACJGB010000172 DACJGB010000000
VERSION     DACJGB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.183"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            493..714
                     /locus_tag="IYI34_003062"
     CDS             493..714
                     /locus_tag="IYI34_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560862.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="IYI34_003063"
     CDS             715..972
                     /locus_tag="IYI34_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560863.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="IYI34_003064"
     CDS             complement(1108..1710)
                     /locus_tag="IYI34_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAR0560864.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="IYI34_003065"
     CDS             complement(1777..1932)
                     /locus_tag="IYI34_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAR0560865.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DACJGB010000173         2003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.184, whole genome shotgun sequence.
ACCESSION   DACJGB010000173 DACJGB010000000
VERSION     DACJGB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.184"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..378
                     /locus_tag="IYI34_003066"
     CDS             <1..378
                     /locus_tag="IYI34_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAR0560866.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IYI34_003067"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IYI34_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAR0560867.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IYI34_003068"
     CDS             1859..>2003
                     /locus_tag="IYI34_003068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAR0560868.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACJGB010000174         1981 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.185, whole genome shotgun sequence.
ACCESSION   DACJGB010000174 DACJGB010000000
VERSION     DACJGB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.185"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..159
                     /locus_tag="IYI34_003069"
     CDS             <1..159
                     /locus_tag="IYI34_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAR0560869.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="IYI34_003070"
     CDS             242..859
                     /locus_tag="IYI34_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560870.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="IYI34_003071"
     CDS             1023..1355
                     /locus_tag="IYI34_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560871.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="IYI34_003072"
     CDS             1679..1840
                     /locus_tag="IYI34_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560872.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..1981
                     /locus_tag="IYI34_003073"
                     /pseudo
     CDS             1872..1981
                     /locus_tag="IYI34_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          705 a          250 c          400 g          626 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 g
//
LOCUS       DACJGB010000175         1840 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.186, whole genome shotgun sequence.
ACCESSION   DACJGB010000175 DACJGB010000000
VERSION     DACJGB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.186"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            465..1637
                     /locus_tag="IYI34_003074"
     CDS             465..1637
                     /locus_tag="IYI34_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAR0560873.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          640 a          341 c          301 g          558 t
ORIGIN      
        1 aagttcaaaa gatcaagtgt gcgcttcgct cccactcccc tcgacgtttt agtagccttt
       61 ccctcacttc gttcagtcca agccaactaa aaagttttcg ggatacattc tccttctctt
      121 cctaataatc aattaaaatt ttaatcttta tatttctgct aatcactcag gaaacagtac
      181 agaaaaacaa acacgtatca tagatataaa tgtaatggca tagtgcgggt tttattttca
      241 gcctgtatca tagctaaaca aatcgagttg tgtgtccgtt tttgggcgtt ctgctagctt
      301 gtttaaagtc tcttgaatga atgtatgttc taagtcaaaa aactctgaca gcgcctttat
      361 atagataaag tccgtataat tgtgtaaaag taaaaaggcc atataacagt ccttttacgg
      421 tacaatgttt ttaacgacaa aaacataccc aggaggactt ttacatgacc caagtacatt
      481 ttacactgaa aagcgaagag attcaaagca ttattgaata ttctgtaaag gatgacgttt
      541 ctaaaaatat tttaacaacg gtatttaatc aactaatgga aaatcaacga acagaatata
      601 ttcaagcaaa agaatatgaa cgaacagaaa accgacaaag tcaacgaaat ggctattatg
      661 agcgcagctt tacgacacgt gtaggcacgc tagaattaaa agtacccaga acacgtgatg
      721 gccatttttc acccacagtg tttgaacgtt atcaacgaaa cgaaaaagcc ctcatggctt
      781 caatgttgga aatgtatgta tcaggcgttt caactcgtaa agtatcaaaa attgtggaag
      841 aactttgtgg taaatccgtc tctaagtcct tcgtttctag cttaacagaa cagctagaac
      901 ctatggttaa cgagtggcag aatcgtttat tatcagaaaa aaattatcct tacttaatga
      961 ccgatgtact ctatataaaa gtacgagaag aaaatcgagt actctcaaaa agctgtcata
     1021 tagcgattgg aataaccaaa gatggcgacc gtgaaattat cggcttcatg attcaaagtg
     1081 gcgaaagcga agagacctgg acaacatttt ttgaatacct aaaagaacgc ggtttacaag
     1141 gtacggaact cgttatttct gatgcgcaca aaggattagt ctctgccatt agaaaatcct
     1201 tcaccaacgt aagttggcaa agatgccaag ttcacttcct aagaaatatc tttaccacca
     1261 ttcctaaaaa aaattcaaaa tctttcagag aagctgttaa aggaattttt aagttcacag
     1321 atattaactt agcgcgtgag gctaaaaatc gattgattca tgattatatc gatcaaccaa
     1381 aatattcaaa agcttgcgca tcattggatg atggattcga agacgccttt caatataccg
     1441 tacaaggaaa ttcccacaat cgactaaaga gtaccaatct aattgaacga ctgaatcaag
     1501 aagtacgcag aagagaaaag attattcgca tcttccccaa tcaaacatca gccaatcgct
     1561 taattggagc cgttcttatg gacctacatg atgaatggat ttattcttca agaaaataca
     1621 tcaattttga taagtagaaa tggtaaaaac attgtatagc attttacaca ggagtctgga
     1681 cttgactcac ttcctttatt atttttcatt tttttaaact atttaatact aatgtctttt
     1741 ataatagctt ttcatatatt atataatcaa tctttataag tccttttata aatttctttt
     1801 ctaccatttt cgataaattc ctgtttaata tttttaattc
//
LOCUS       DACJGB010000176         1838 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.187, whole genome shotgun sequence.
ACCESSION   DACJGB010000176 DACJGB010000000
VERSION     DACJGB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.187"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..974
                     /locus_tag="IYI34_003075"
     CDS             <1..974
                     /locus_tag="IYI34_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR0560874.1"
                     /translation="LPINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARN
                     VAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDK
                     ALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGY
                     LSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFG
                     EIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGY
                     HVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1164..1237
                     /locus_tag="IYI34_003076"
     tRNA            1164..1237
                     /locus_tag="IYI34_003076"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1198..1200,aa:Ile,seq:cat)
BASE COUNT          607 a          317 c          346 g          568 t
ORIGIN      
        1 tattacccat taatacgttt gcttatacta ttaatgacga gtataattta gcgccgaatc
       61 aaggagactc cagattagca attcctaaca aaattatttt gcacgaaact ggaatagatg
      121 caccagcaag aaacgtagcc gccaacatga aaaataatta taacggaagc aattcttata
      181 ctacagatgt tattggtgac ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt
      241 cgtggggagc tggtaacgct aatccttatg cgcctgtaca gattgaatta cagcgcacat
      301 atgataaagc attgtttgaa aaaaactatc gagcttatat tgaatataca agagatagtg
      361 caaaaaaata tggaattcca ttgactcttg atcaaggaac ttctttattt acaaaaggaa
      421 tcatttctca tttgtgggtg acaaattatg tttgggggaa ccacacagat ccatatggtt
      481 acttatcgca aatgggagtt agcaaagaaa agcttgctta tgatttagct catggattta
      541 ccgatgaaaa tccaacaact tctgaaaaca agcctgtcat tgatccaaca cgagctggtg
      601 cagctaatcc tacgctgaca gatggaacaa atcacgccca cattgatcag tttggggaaa
      661 tcgaaaacgc aaacttgcat gtggctggat ggcacattgc taactataaa tacgagtata
      721 ttttcattat ggactacaat actgggaaag aattagctcg agtaagagct gatggaattt
      781 atagaccaga tgtaaaccaa gcttataata cttctggaaa tgttggttat catgtatctt
      841 tcaatatgcg taattttcct agtaagaaag tctatgtaat gatgcgggca acgaatgatc
      901 cagagggaaa cactaaaggc ggtgcgcaag atttccatga caaacgttgg tatttaaata
      961 ttcctaaacg ataaaaatag ctcctcgttg aggagcagta cataactata ttgacaacta
     1021 taaaaattat tcgataaaat agtgatgtta tcgcatatct tcactatcac ccataaatag
     1081 tcacactcca agctatgcga taacaggttt gttgccacac attctactgg ttgattgttt
     1141 atggctttat gtggcaacaa ccagtaccct tagctcagtt ggtcagagca gacggctcat
     1201 aaccgtccgg tcgtaggttc gagtcctaca gggtacatta acgtagccat ttgaatcgtt
     1261 ctgtgttaga atttttttga agagtattat acaagctaaa gcttttcttc attgccactc
     1321 aaatgagtgg cttttttatg tatcctttta tggattaatg aaaggatgtt tcacatagtt
     1381 atacttctgt atatttgaaa agttttactt tgatttttaa atagaaagac atttgggtta
     1441 aattgtgaga taataataaa gaagagttta aagcgcaccc caaaccactt ccccataagt
     1501 gtgttacgct ttaaactctt ttatatttga agccattaaa aagcatacca tatttttgaa
     1561 aaaaagtgag aaaaaaggct tacaattgga gtggtagtta attagtgact tatttttgat
     1621 tttatagcac tgatactata aaatatagat atcatcatat tacacaatct taatactaac
     1681 ttaaaaaata tctcctttca caagtatggt gataaaattc gttccgggct acctttttag
     1741 gtagcctact ttaatcttta tacctttctg gatcaacgaa agtatacttt atatagtcat
     1801 aacgccgatg atcgctacgt gcgtccggca cgtcagtc
//
LOCUS       DACJGB010000177         1732 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.189, whole genome shotgun sequence.
ACCESSION   DACJGB010000177 DACJGB010000000
VERSION     DACJGB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.189"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..1026
                     /locus_tag="IYI34_003077"
     CDS             <1..1026
                     /locus_tag="IYI34_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560875.1"
                     /translation="PTATPYQPNLLDAPYYLSKVALGENIADPTVSFPIKTSAYRLYG
                     VNMLEEFKVGQRYTITIKGTKPATQDFWVFNSGDVSLGKMKPVEGLSDIWTLTFTPNA
                     VNSSTPKLLAIYQTPQSTAGACQIDWLKIEKGDTRTPNIGEYKYRGTGMRDSNNPKDY
                     VWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTK
                     ANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSS
                     GGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYT
                     FAITYTI"
     gene            1189..1635
                     /locus_tag="IYI34_003078"
     CDS             1189..1635
                     /locus_tag="IYI34_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560876.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1637..>1732
                     /locus_tag="IYI34_003079"
     CDS             1637..>1732
                     /locus_tag="IYI34_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAR0560877.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT          623 a          301 c          360 g          448 t
ORIGIN      
        1 ccaacagcca caccatacca gccaaattta ctcgatgcac cgtattattt gagtaaggtg
       61 gctttgggcg agaatattgc tgatccaaca gttagcttcc caattaaaac gagtgcatac
      121 cgtttatacg gtgtgaacat gctagaagag tttaaagttg gtcaaagata tacaattaca
      181 ataaaaggaa ctaaaccagc aactcaagat ttttgggtgt tcaattctgg agatgtttca
      241 ttaggaaaaa tgaaaccagt agaaggctta agtgatatat ggacgttaac ttttacacct
      301 aatgcagtta atagtagcac acctaaacta ttagctatat atcaaacacc acaatcaaca
      361 gcaggcgcat gccaaattga ctggcttaag attgaaaaag gcgacacccg aaccccgaat
      421 attggtgagt ataaatatcg tggtactggt atgcgtgatt caaacaatcc aaaagattat
      481 gtttgggatc tagcaccaga atatgtcgaa gataatttgg ccacagatat taaaatttct
      541 gaaattactg gtaaagcaaa caattatacc gatgggaaag tatcggagat taattcgcag
      601 ttgactgctt caattaatga agtagacacc acagctaagg atgctcaaac aaaagcgaat
      661 gctaatgcga ctgctataga tgaattagac aataagatcg atgaacgcat taatgataca
      721 gctactacca cattaacagt tacaaacggg aataccggat cagcaaagct ttatcgtgaa
      781 ggaaaaacag tttctatata ttttgtggct ttaaacggaa aaagcagtgg tggaaatgat
      841 tcaacgatac taacaattcc agaaggctat cggccaccaa ttagttttga gcaactggtt
      901 ggctcgatag accgttctac tttgaacagt gctcagttat ctattggtgc agatggagcc
      961 attaaatggc gaagaaactc aagttatgga tcggattata cctttgcaat tacttacacg
     1021 atttagaaaa gcgtgaatcg atatgaaggc agcatataga ccaattgaac cttacggatt
     1081 cgagcaaatc attgtgaatg atgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1141 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1201 tggaaatatg gacgtactgg cggagagtac gcaggaaaag tattggacga catgcttgta
     1261 tccgttcctt acacagatca gccaccgctt gaaggaattc gtgctgatgg cgaaccgtta
     1321 acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat ggattatttt agcgaacgca
     1381 ctagatcaca acgatttaaa caatctcaaa gcaatgtatg agtcgttaga aaatgagaac
     1441 ggcgatttaa aacagatcaa tgccaaactc atgctaagcg atgtagcaat taaacaggaa
     1501 aatactgcat tgaaagaaaa agcggatagt ttagcacaaa tcaattcaaa aatgatgctt
     1561 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1621 aagggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1681 tttcactaac gaacaagtaa tggttttcgt tccgttgtgc attactgaag aa
//
LOCUS       DACJGB010000178         1654 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.190, whole genome shotgun sequence.
ACCESSION   DACJGB010000178 DACJGB010000000
VERSION     DACJGB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.190"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..1144
                     /locus_tag="IYI34_003080"
     CDS             <1..1144
                     /locus_tag="IYI34_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAR0560878.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1425..1654
                     /locus_tag="IYI34_003081"
                     /pseudo
     CDS             1425..1654
                     /locus_tag="IYI34_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          591 a          293 c          356 g          414 t
ORIGIN      
        1 taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta ctagcgcatc
       61 agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg gtgtcaaacc
      121 aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc gtggatacaa
      181 ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa tcgctgtcgt
      241 aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg ggaaagaagt
      301 cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg agatgacaga
      361 tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg caaaaatcga
      421 agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag aaatcccagc
      481 agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa aagggatcgt
      541 cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga atcttccaga
      601 tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca aaaaaatgcc
      661 gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac atctttttga
      721 agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa gtgtctttga
      781 ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag gagtagatgt
      841 acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag aacaaggaaa
      901 tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc taggaggagc
      961 agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag cgatccaaaa
     1021 caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag gctttgaccg
     1081 acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac catttgtaga
     1141 ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat caagtaataa
     1201 gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta ttaccgcaga
     1261 tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat ttgtgtaccg
     1321 acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt tatggttaaa
     1381 tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa aggcgattgg
     1441 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg gagaagcaca
     1501 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
     1561 aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca tacacgcgta
     1621 aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DACJGB010000179         1630 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.191, whole genome shotgun sequence.
ACCESSION   DACJGB010000179 DACJGB010000000
VERSION     DACJGB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.191"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(52..1518)
                     /locus_tag="IYI34_003082"
     CDS             complement(52..1518)
                     /locus_tag="IYI34_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAR0560879.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          547 a          336 c          294 g          453 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta
//
LOCUS       DACJGB010000180         1622 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.192, whole genome shotgun sequence.
ACCESSION   DACJGB010000180 DACJGB010000000
VERSION     DACJGB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.192"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            1..117
                     /locus_tag="IYI34_003083"
                     /pseudo
     CDS             1..117
                     /locus_tag="IYI34_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          614 a          189 c          327 g          492 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacga ttatcgaaca
       61 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaattt aatttgacaa
      121 ttaagaaagt attaacttgt tttgtgaaaa agagaatttg aaaaacgatt ggcgaacaaa
      181 tgctcggtag gtatggtata aatacagagc taattggtgt gatgaaatca gatattcgcg
      241 tgagattcag taaagtagga gttcgaaaac agctaggtga caaacagtat tgctttggta
      301 cttcggaatg gtttatcaaa ttcttatcaa cagttcactt ctctttatgt ttgagtgatg
      361 attcgttagg ttattgcttg gaaagaccgg atgagagtag aagagtttaa ttttataaag
      421 tgaaaagagg taatgtacga tatacgtagg gctgaaccaa tagaggataa gcaaacgatg
      481 ttacaggcgc aaaattcata gcttactgtt ttggagataa cagaagagga aataatgcag
      541 aagtaaggcg aaaaaaggac ggaagcctaa cagcatatga aataaagaaa aatattataa
      601 ctactgataa atcggtattg agcaaagtac taataggagt caaaaacatt cataagctat
      661 gagtgtttct tggctacttt tttattttta tgtatctaca aaagtttcca ggggcagaac
      721 ctgtgattat taatggaaga acctcaagta ttgtcttaaa tcattgttat gaaaatggat
      781 taaaagagtt catcaaggtg tgattaacaa gttacttttc attacatcga atttttcaga
      841 agttgcttat attggtgatg atattaatga tttatcatgt atgacatctg tgaagaagcg
      901 ggaggaatag tagaatgtcc aaaaaatgcc acaagaaatg agattgaatt atctgatttt
      961 attgtaactc acagtggtgg tgacgaatct gtgatagagt ttattgagtg gatattggaa
     1021 tcgatatagt gtagtagtac agaaaaatta tcatacaatt aatactaggc ataagaatat
     1081 tggcatattc tagagttaaa agtaactatt cgaaaaggat aaggttagaa ttttttgttt
     1141 tgccagtgga agaatataaa aagggcattg gaaattaata tattagaatg ctaagcggag
     1201 aaaaaaatat atgaaaagta cgtccttgtg atgggatgga aaaatacact tatttcaaac
     1261 aacgataata taattatgtg aaccgaagta tactcgcttc taattcaatc ctcaaatccc
     1321 gacagtcgcg acttcaaaaa agcgtatagt tttaatgtat ccccatatga tccgaaaaat
     1381 ggtaatttac aattaacagt ttgatgatgt tgaagatgtg gaaatgctga aattaccatg
     1441 atgagaaaca cattaattaa agggaattaa ttaactagaa tataatctga ttcaatttga
     1501 attaagcaaa aaaaggctga gttttaaata atcgcgaatt gtaaaattaa gttagaaaaa
     1561 taaaaaggca tttatggtac actcaaattg tatttccgac gaaagaaaac aaaggagtgt
     1621 aa
//
LOCUS       DACJGB010000181         1527 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.193, whole genome shotgun sequence.
ACCESSION   DACJGB010000181 DACJGB010000000
VERSION     DACJGB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.193"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            289..696
                     /locus_tag="IYI34_003084"
     CDS             289..696
                     /locus_tag="IYI34_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560880.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="IYI34_003085"
     CDS             710..1111
                     /locus_tag="IYI34_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560881.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="IYI34_003086"
     CDS             1113..1484
                     /locus_tag="IYI34_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560882.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          578 a          186 c          264 g          499 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta tttttttatt taatctt
//
LOCUS       DACJGB010000182         1489 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.194, whole genome shotgun sequence.
ACCESSION   DACJGB010000182 DACJGB010000000
VERSION     DACJGB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.194"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            44..1411
                     /gene="nhaC"
                     /locus_tag="IYI34_003087"
     CDS             44..1411
                     /gene="nhaC"
                     /locus_tag="IYI34_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAR0560883.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
BASE COUNT          391 a          283 c          327 g          488 t
ORIGIN      
        1 tttttcatgg cagggccttt tcatttgaaa taggagatcg acgatgaata aaaatatttc
       61 tttaaaagaa agtatcgctg tattgatcgt gttgttggca ctcctaggag tactgatcat
      121 cggattccag ctctcgccac atatcccgat ccttgcagct ttcatgtgtt tgttgtttta
      181 tggcagattt aaaaagttca catgggatga gatccatgac gggatcatca aaggaatcac
      241 tccagggatc attccaatcc tgatttttct tttgattggt gtcttagttg cttcatggat
      301 tttatctgga accatcccta cgattatggt ttatggattt aaacttgttt ctgtgcgttt
      361 ctttttaccg acagcatttc ttgtttgtgc aattgtcgga gtgactgtcg gcagttcatt
      421 tacaacgatt tcgacgattg gaattgcttt tttggggatc ggtcatttac ttggattcaa
      481 tgatgcaatg actacaggcg cagtcgtttc cggtgccttt ttagggaata actgctcgcc
      541 tttatcagat acgaccaact tggcagcggg tattggagga gttaatctat tcgaacatat
      601 cgctggtatg cgttatacgg atttcccagc attcgtcctt tcgttgattt tttatacgat
      661 attaggacat aacagtcatt cagccgatct agctagtatc aatgaaatga tccaaaacat
      721 gaaagcgagc ttttggatct ctccttggac attgattcca ctagttattt tatttggggg
      781 ggcaatcaaa aaaattccgg ctatccctac attactagcg ggatcgacag ctgctctcgt
      841 tctttctttt atccatgaga aaagtttgac ggtcggaaaa gcagcagaca tcttgatgaa
      901 tggttatgtc gcaaatacac ctgatccaaa gctagatgaa ctgctttctc gaggcgggat
      961 catgagtatg ttaggttctg ctagtttgat tttacttgca ttggcattag gtgggttact
     1021 gatcaaatat ctgatcgttg ataccatcgt tcaagaatta aaagaaaaga tggaccgacc
     1081 ttctcgttta gtcgggttta ctgccttgag ctgtatcgga atcaatctga tcgttggaga
     1141 acagtatctg tccatcattc ttccaggaga aacattcaag cgttcttttg atgacgttgg
     1201 cttatcaaaa acctatttga cacgaacact agcggattca ggtgcgaccg tcaattctct
     1261 cgtaccgtgg ggagtcagcg gcacattcat tatgggaacg atgaaagtta gcgcgttgca
     1321 ttacttgcca ttcgcctttt tctcgattct tgcacctgtc tttacaatat taggcggttt
     1381 tttattgaat cgtcgacgtg taaaaaattg aaggaaggaa aaaagaggct gggacagaag
     1441 cgtttaactc caagaaataa gaagaaactc aagaaaattg ctttttaaa
//
LOCUS       DACJGB010000183         1486 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.195, whole genome shotgun sequence.
ACCESSION   DACJGB010000183 DACJGB010000000
VERSION     DACJGB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.195"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            30..707
                     /locus_tag="IYI34_003088"
     CDS             30..707
                     /locus_tag="IYI34_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAR0560884.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
     gene            complement(844..1260)
                     /locus_tag="IYI34_003089"
     CDS             complement(844..1260)
                     /locus_tag="IYI34_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560885.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1263..>1486)
                     /locus_tag="IYI34_003090"
     CDS             complement(1263..>1486)
                     /locus_tag="IYI34_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560886.1"
                     /translation="ELTNKTGEKWSRNKYLKVLIESDFDRQLTTYKKDQFDLLLDKFI
                     AIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          464 a          221 c          264 g          537 t
ORIGIN      
        1 cggaattaac agtcaaatat caaaatgaat tgaatttagt accgctgaaa aatttcaatg
       61 ctaagcagat ggatttattt tttgcattat gcgctcgtat gaaagatcgt ggtttgaaaa
      121 aagttagatt ttctttcgag gaattaaaag agttaagtga ttataagatg actgctacaa
      181 aagcctttgt cggtgattta gaaaagctct ataaagatat gcttaatctt acttaccgta
      241 ctgaagatga ggattctatt cgctactttg ttttgtttac tggttttgaa atcaataaaa
      301 caaaaaagtt tgttgaagtc agtgttaatc cggacctaga atatattatt aatggtttaa
      361 cagctgaatt tagtagattt gaattatctg ctttcacgtc gttgcgttct acgtatgcaa
      421 aaactctatt ccgactgctt atgcagtacc gttctactgg ttattatgta gttcgtattg
      481 aagagtttag gagcttaatg gatatcccag aatactatca aatgggtaac attgatcaaa
      541 aagtattaaa tcctgcatta cgagaactaa aaaaatactt caatgatctt aaagtcacaa
      601 aaataaaagc aaaaaagggc aataaaatcg ccaaattaga atttactttt tcaagcctaa
      661 cgtcagattt gccaaagatt tctctgcgta actggttaga agaataaata aaaaagaact
      721 cgcccccctt acattaagca agtagacaag tcccttacaa ccgactaaga ttgctagtac
      781 attctagcat attgtatggg gcttctaaat aaaaaacaga taggaataac tcctatctgt
      841 tttttatcgt tttagaaaat ttgttgggtc gaaatttcta gaagtgactg tttgactggt
      901 ttctttttga tgattctttg gtcgatttcg atttgatgga tttagtttag cttcgatttt
      961 ttctttcact tcttgatata ctttagaatt tgtgccaata agaacgcctg tatcttttag
     1021 atataaatca gcaaattctg cgatgatttc agtcatgatg gaaatttctt ttcccatagc
     1081 atttagtttt gcctgattgc tggtagtggt cgtgttggta tcgggttcat aactcataca
     1141 taaaaagcga atggcttccg agtagttatt gatatctgga tttttttcga tgatggaatc
     1201 taaaacgtca acatgactag ggtacaaagt aacatgtttt tggacagaag tttttctcat
     1261 ggttatatca ttcctccgat aagcatttca atgatttgat tatttgtgta gacatactcg
     1321 cttaatattt gagtattttc tgtttggata gcaataaatt tatcgagcaa taaatcgaat
     1381 tggtcttttt tgtaagtcgt aagttgacgg tcaaaatcac tttcgattaa aacttttaga
     1441 tatttatttc gactccattt ttctcctgtt ttattcgtga gttctt
//
LOCUS       DACJGB010000184         1426 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.196, whole genome shotgun sequence.
ACCESSION   DACJGB010000184 DACJGB010000000
VERSION     DACJGB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.196"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..721
                     /locus_tag="IYI34_003091"
     CDS             <1..721
                     /locus_tag="IYI34_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAR0560887.1"
                     /translation="LETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTT
                     QDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWR
                     EEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVRHESIS
                     ERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGAL
                     IIATVDDPKQNVFTLEKVGVI"
     gene            721..1338
                     /locus_tag="IYI34_003092"
     CDS             721..1338
                     /locus_tag="IYI34_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560888.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          532 a          188 c          296 g          410 t
ORIGIN      
        1 attagaaaca aaaattgtgg atgctaaaaa agggaaaatc aaatatattt ttgataaata
       61 ttccatgcag tgtttgcatc gacaaacagc caatttcatt atatttaaag gagaagactt
      121 gattggaaca actcaagact tctcttattt tgtcatcaat gctgtttcaa aaacagaagg
      181 agaaatgggt tcttattggc aatcaattga agatttaatt gctgatatga cagattttat
      241 taatgaaaat aaaggcgatt ttacggactg gatgaatgaa aggaaagaag aatttgatcg
      301 ctggagagag gaacaggaga atagtttcca agattggaga gaaggacaag aatcagatta
      361 cttgtcatgg tttgaatcaa tcaaggatat tttgaagtct atcgatccag gtggagtaat
      421 gttagccgaa ttaatggatg cacgtgtaga tcttcaaggt gtacgccatg aatcgatttc
      481 tgaacgtttc ctatccgatc taaattattt gtatcaaaaa atgaaagcag cacttttcac
      541 gattgaatac ggtgaaattg aagtgactga tattcttcaa gacgatctct tttcagataa
      601 tcacgaagtc gaaaaaattg aaacagtaga atttccaatt gaagaaggag ctttgattat
      661 tgcaactgtt gatgatccaa aacagaatgt tttcactctt gagaaagttg gggtgatttg
      721 atggttaaag taaagagaat gatggaaact gatgaatctg gtgttaaacg tcaatttatg
      781 ccaattacac atgtctctgc agttttaggc ttagaaaaaa tgatatcagg acaatcaaaa
      841 gttctttcgg ttaatggtaa atatggtgca gtcattttaa cgaaagctga tttaggatta
      901 gagaatgcaa ttactgaact accttatgca agcgagacaa gcgatggtat tcttactgct
      961 gaaatgtttc aaaagatcgt aaatggagaa ggtggaacat atattttgcc gattgctact
     1021 cctgaacaat taggaggtat aaaagttggg gaactcttag aagttactga agaaggtgtt
     1081 ttatctgcaa ctaagcaaac agacttcaat ttctctgaag aattaaaagg aaaattagaa
     1141 tctctaaaaa tattaaaagc aggtgcaaat atatcaattg cagaagatgg aacaattagt
     1201 tctacaggtg gtagtgggac tggtggtgtt aatcagtcat atgtagatca aaaattccaa
     1261 gaagctgtaa atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa
     1321 aaaattgggg aggtatagag aatgacagat atcgtaaaaa taaaacaaag caatgtacag
     1381 gtttatccac aaactcattg gaatgctata gaaggcaaac caacaa
//
LOCUS       DACJGB010000185         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.197, whole genome shotgun sequence.
ACCESSION   DACJGB010000185 DACJGB010000000
VERSION     DACJGB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.197"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(150..1337)
                     /locus_tag="IYI34_003093"
     CDS             complement(150..1337)
                     /locus_tag="IYI34_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAR0560889.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACJGB010000186         1364 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.198, whole genome shotgun sequence.
ACCESSION   DACJGB010000186 DACJGB010000000
VERSION     DACJGB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.198"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            29..1330
                     /locus_tag="IYI34_003094"
     CDS             29..1330
                     /locus_tag="IYI34_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR0560890.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          443 a          274 c          252 g          395 t
ORIGIN      
        1 ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca aactactact
       61 tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca ttaagaatat
      121 caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc ctttgtgtaa
      181 acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc tcattcaact
      241 taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct accactgtcg
      301 tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact gctttatcgc
      361 taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa tgacgacaat
      421 tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt ctctgatccc
      481 catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg aatttcgttc
      541 actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata ctggaaaatt
      601 actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta tgcaatttga
      661 acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt cttatgcctc
      721 tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc acattgtcaa
      781 acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct ttgatcgaaa
      841 cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc ttctgatcaa
      901 acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg gttgggcgta
      961 tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat tacgaatcgc
     1021 ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg acactttctt
     1081 taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta taaaaaaagc
     1141 ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt caaatgccaa
     1201 aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg gctttcgaag
     1261 ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct atgaatcaaa
     1321 aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DACJGB010000187         1340 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.199, whole genome shotgun sequence.
ACCESSION   DACJGB010000187 DACJGB010000000
VERSION     DACJGB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.199"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..112
                     /locus_tag="IYI34_003095"
     CDS             <1..112
                     /locus_tag="IYI34_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAR0560891.1"
                     /translation="ASITADDSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            118..804
                     /locus_tag="IYI34_003096"
     CDS             118..804
                     /locus_tag="IYI34_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560892.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            811..>1340
                     /locus_tag="IYI34_003097"
     CDS             811..>1340
                     /locus_tag="IYI34_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HAR0560893.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAK"
BASE COUNT          507 a          216 c          274 g          343 t
ORIGIN      
        1 ggcttctata accgctgatg acagtaaatg gaccaagaga gacttgggca ttctaaaacg
       61 aggcgttaac tggattgaag atcagtacag agaagaaaca aaagagaagt gatatgagtg
      121 tttaaaccat tgatcgattc atattcagcg gttctgaaaa agttcaaagg aaaagacata
      181 ggtgcaacga tcaatgaaga agtgaacatt gatcgactaa agacgatgta tgacggatat
      241 gatggcgatc ggattattga agttcgattt attgatccac gtcgatttac tgtgcagcaa
      301 cgaaacttca tctatgcgct gataggcgat attttcatcg atacaggcat gccaacggac
      361 ttctggaagg aattctttta cttccgtttt gaaggcgtca cagggcgcaa aataagcctc
      421 aaagacgaat cgaatacaac tgtgagtgat gctaatgtct tagcaaatat catcttagat
      481 ttcatctttg aacatcatat tcctttcaaa gaaggctatg agattttacc agcgaatcaa
      541 gaatattact tctacaaatg catcacaaaa agagtctgct gcatctgtgg caaaacagga
      601 gctgacatcg atcactttga caaagcgcta ggaagacgaa agcgcaaaga agttgatcat
      661 tcagagtaca catttgcagc actctgcagg attcatcaca cggagaaaca caatataggt
      721 gtgatcaatt tcaaaaataa atatcaaatc aaagggatca agttaaacca ggaaacaatt
      781 aagaaactta gaataggagg gtaaaaaata ttgtctgaca acaaacgcta ctactattta
      841 aaactaaaag agaatttttt cgatagtgac gagatggttc tcttagaaag tatgccagat
      901 ggctatattt attctaatat tcttctcaaa ctttatttaa gaagtctaaa acacgaaggt
      961 aagttgatgt ttaatgacag gattccattt aactctacaa tgctcgcaac tattacaaga
     1021 cattctgtag gagtcgtaga aaaagcggta caaattttcc gtgatttgca gcttattgac
     1081 gtattagata acggagcaat ttatatgtct gatatacaaa gtttcattgg aaaatcctca
     1141 actgaagcag atagaaaaag agaatacaga aagaaaatag aagaggcaaa acggaattta
     1201 ataactggag gacaagtgtc ggacaaatgt ccagacaaaa ctacaccaga gttagagata
     1261 gagatagaga aagatataga tatagataaa gaagaaaaga aaggtaagta ttctgacgaa
     1321 cacttacgcc ttgctaaaaa
//
LOCUS       DACJGB010000188         1338 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.200, whole genome shotgun sequence.
ACCESSION   DACJGB010000188 DACJGB010000000
VERSION     DACJGB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.200"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            108..794
                     /locus_tag="IYI34_003098"
     CDS             108..794
                     /locus_tag="IYI34_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560894.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            797..>1338
                     /locus_tag="IYI34_003099"
     CDS             797..>1338
                     /locus_tag="IYI34_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAR0560895.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSN"
BASE COUNT          504 a          223 c          282 g          329 t
ORIGIN      
        1 accgctgatg acagtaaatg gaccaagaga gacttgggca ttctaaaacg aggcgttaat
       61 tggcttgaag atcagtacag agaagaaaca aaagagaagt gatatgagtg tttaaaccac
      121 taatagattc atactcggcg gtactaaaga aatttaaagg taacgacatt ggtgcaacaa
      181 tcaacgaaga agtaaatatc gaacggctga aaacgatgta tgacggctat gatggcgatc
      241 gagtcattga aattcgtttt attgatccta gacgtttcac cgtacagcaa cgaaacttca
      301 tctatgcact cataggcgat attttcatcg atacaggcat gccaacggac ttctggaagg
      361 aattcttcta ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg aaagacgaat
      421 cgaatacaac tgtgagtgat gccaatgtct tagcaaatat catcttagat ttcatctttg
      481 aacatcatat tcctttcaaa gaaggctatg agattttacc tgcgaatcaa gaatattact
      541 tctacaaatg catcacaaaa agagtctgct gcatctgtgg caaaacagga gctgacatcg
      601 atcactttga caaagcgcta ggaagacgaa agcgcaaaga agttgatcat tcagagtaca
      661 catttgcagc actctgcaga atccatcaca cagagaaaca caaaataggt gtgatcaatt
      721 tcaaaaataa atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa
      781 ggataggagg ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga
      841 cgatgaaaaa atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg
      901 gattcgatta ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag
      961 gaacatgcct tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt
     1021 tcgcttagcg ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact
     1081 aattgccatc agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct
     1141 aaaaaatgct gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt
     1201 ggaaaatgac gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa
     1261 agatatagat aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa
     1321 aaagttgcaa agtaattt
//
LOCUS       DACJGB010000189         1337 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.201, whole genome shotgun sequence.
ACCESSION   DACJGB010000189 DACJGB010000000
VERSION     DACJGB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.201"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..>1337
                     /locus_tag="IYI34_003100"
     rRNA            <1..>1337
                     /locus_tag="IYI34_003100"
                     /product="23S ribosomal RNA"
BASE COUNT          358 a          293 c          406 g          280 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggactta
//
LOCUS       DACJGB010000190         1324 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.202, whole genome shotgun sequence.
ACCESSION   DACJGB010000190 DACJGB010000000
VERSION     DACJGB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.202"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            211..753
                     /locus_tag="IYI34_003101"
     CDS             211..753
                     /locus_tag="IYI34_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560896.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            909..1283
                     /locus_tag="IYI34_003102"
     CDS             909..1283
                     /locus_tag="IYI34_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAR0560897.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          536 a          151 c          258 g          379 t
ORIGIN      
        1 agccgattca aattgatgaa gcagatccca aagggctgct tcatcaattt tttcgtaagt
       61 gtccgtacac ttactgtgct tgctcatata ttaatcgatc atatactaat cgtgtaagaa
      121 caactttaag cataatactt atattgttat accatagttt ggaaaaataa aaaattatat
      181 ttcatcaacg tttcataaaa ggtgtgtaga atgttattga aagtagctat aaaatgctat
      241 tgtgtaaaaa ataaaggagg aataatttat gaaaaaatag taccaagagg agcatgccta
      301 ttacttgcat tatgtttgag cttaagcgca gttgggtgtc aaaaaagtaa tgaaaacaat
      361 aaaataaaag aaggacaatc cataagttct gaagaggaag gcatgtcaat taatgcaaat
      421 ggcaaaaatg aaacttttaa gccatcagat tatacattgg aggcaaagaa agaatatgtt
      481 tatgaatatt tagggcttaa atttaaactc tcagacaaat ttagaaatta tatagctgat
      541 aaaaagatag cgatgttaga tgatcaaagt cctatagata aagaactaaa gtatgctatt
      601 ttgacatttg agaaaatgac agaagaacaa aagaatgcag ttatagaaaa gatgggcgat
      661 ggatataaaa attggcaaaa tgagcttgaa agaattggga ctattggtat atttgaaaaa
      721 aatacatcag aagaaaaaaa tctaaaatta taaaatgtga tactcatact aaaataggag
      781 tttcaagtga tggaaaatac gattgctatt taagcacaaa tagcggagct gaaagtaatc
      841 ttctggatga attaaaaaga actgagattc aaattataga taaaaaagaa cgtcctaaaa
      901 atggttttgt gctatcagaa aaaactgatt tagaaaatac agaagcattt aacaaagaat
      961 cggttaaaga tttacgtaaa ctaccaacaa aggacataaa tggcaaggat tttacaagta
     1021 aagattttga gaagtatgat cttactatgg taaatgtatt tgcaacttgg tgtacagctt
     1081 gtgtaaagga aattcctgat ttggttgagg ttcagaatga aatgaaaagc aagggtgtaa
     1141 atatcgttgg agttgttaca gatcctgttg atgataacgg tgaaaacaag gaagcaattg
     1201 aaaagtcaaa gttaatacat gaaaagacaa aggcttcata tcctttctta atgccagatg
     1261 tggaatggca acactttttt tgacaaaaat tataaggtgt tgtgaatgct gcggtgttat
     1321 ggct
//
LOCUS       DACJGB010000191         1273 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.203, whole genome shotgun sequence.
ACCESSION   DACJGB010000191 DACJGB010000000
VERSION     DACJGB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.203"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            32..529
                     /locus_tag="IYI34_003103"
     CDS             32..529
                     /locus_tag="IYI34_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560898.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(566..1249)
                     /locus_tag="IYI34_003104"
     CDS             complement(566..1249)
                     /locus_tag="IYI34_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560899.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIK
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKANGVTELAIYQKG
                     KRIMVPLPV"
BASE COUNT          443 a          216 c          224 g          390 t
ORIGIN      
        1 tatagtatac tatgtcaaaa ggaggagtta gatgaacaat caaatcacac tagcgtattt
       61 agattatgat gatctagatg ctttacaagt tgtaagtgtt gtcagttttt atgaatcgtt
      121 tatcgatggc gcagatccac ttgatatgca aggatattta cagacacaat tgacaactga
      181 aatattggca gtagaattgg cacagtcaac cagtaaattt attggtatta aggatcatca
      241 aatactcatt ggttatatga aagtcaatga tgaaaaagat gctatagaga ttcaacggct
      301 ctatctgctc aaagattatc aaaacaaagg gttagggcaa cggttactcg atgaagcgaa
      361 tcgttatgct aaaacgaaac aaaaacgtta tttacgtttg actgtttatg aaaaaaatca
      421 tgcaggtatt cgtttttatg aacgtcatgg atttaaaaaa atcgggatca aacattttcc
      481 tttaggaaaa caagatcgta tttgtccgat tttagaaaaa gaaatctaaa aaaacgtcct
      541 cacttttgat gctgaggacg tttttttaga ctggtaatgg aaccataatt cgctttcctt
      601 tttgataaat cgctaattcc gtcacaccat ttgcttttaa cagagcaaaa agatcatcga
      661 aatggaaacg gaaatgttca aggtaatgcc catctgatcc aacactaaac aaggtaccac
      721 cacgtgaaag atataaatcg atgacatatt catataacgc taaattgtca tacaagtaag
      781 cgctcttagc atttaattcg aatgccaatt tatttttgat tacttgatct agaatcggta
      841 aaaactgcgc ttcatattgt ttaaattcag caacggatag accaaatacc cgaaaaccat
      901 agtcaaaatg agcaaagaca tctgcttcta taacaactag tgcttctgaa agttgtgcaa
      961 aatactgagg aatcacaata gcaggatcca tatctttgac ttcgtcatct aaataatcaa
     1021 attgcccatt atgatgcaca ctgagtaatt ttaaatcgta atctttatcg gctaaatagt
     1081 cgatgatccg atccttttca gaagcgatgt aaccgatttt gatgcctttt aaaacacgat
     1141 tgccaaaacg cttatttaag ctatcgattg tttgactgta catttcataa tcggggatat
     1201 catcgacttt agtaacagga ttggctaagt caaaatgttc agttgtcacg acgtaaccat
     1261 cgtagtttgt taa
//
LOCUS       DACJGB010000192         1235 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.204, whole genome shotgun sequence.
ACCESSION   DACJGB010000192 DACJGB010000000
VERSION     DACJGB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.204"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            648..743
                     /locus_tag="IYI34_003105"
     CDS             648..743
                     /locus_tag="IYI34_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAR0560900.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          367 a          262 c          205 g          401 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tacccttctg
      181 tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag ggttctaaaa aatctttaaa
      241 aaataaataa aataaacgcg cgcgcgcgag caaaatttcc cgccaaaaaa tcacccactt
      301 tttaaaataa acaggttcgc gcatgatatt aaactgccaa tctagctccc tatggtcgct
      361 gtaagaagga gtgatcgcta cgctctcaca ttttacttgc agcagtcgcc tttaggctcc
      421 ttcgccaaaa ataattcatt agggactcct ttgtggcgtc ctgagagctc gtctaagccg
      481 ttctaagaca agattcgata aaaactatgt ccttcgttct aaagcgcctt acaggcggtc
      541 agggcaagcc ctaacaaccc caaggcaata aaaaacttag gcttattttt gcttttttat
      601 tgtataattg attgtcgcct atccgacagt cttgcgactg gaaggaggtg tgtttcgtgt
      661 tcactaactt tatttgctcc cttcttgttg ggcttttaag cgctttgttc agttactggt
      721 tgaataaccg aaacaagaag taaaacttaa ttaaggcgac tcagcccaca aaaaaagccc
      781 cagagaatcc gacctctctg gggatttcgt gtgtttcgtg ttcacttatt tgcaatcaaa
      841 ttataacaga aataaatatt tatttcaaaa atatcctaca aaagtctaaa atccgtaatt
      901 atttcttata tgcaacattt acaaataaag tcgctctgag agctcgtatt tttcgattta
      961 agacgtttta tttagttttt ggtgtatttc attggtccaa gctaaaaaac tcttagaagc
     1021 gattttagag ccatttaaac ccacttaatt atattctcca tgaaattcgc tccatgcgtc
     1081 aaaagctgcg tctgaaattc cgccgtcttc gttcactagt tgcattaatt tttccaacga
     1141 tcgttttctt tcgggtgtca tatttttata ttccaacaat ttttcgtctg ttaaatttgg
     1201 ttatcgatta cacgtgcagc actccccccc gaatt
//
LOCUS       DACJGB010000193         1220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.205, whole genome shotgun sequence.
ACCESSION   DACJGB010000193 DACJGB010000000
VERSION     DACJGB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.205"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            49..1062
                     /locus_tag="IYI34_003106"
     CDS             49..1062
                     /locus_tag="IYI34_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAR0560901.1"
                     /translation="MESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHE
                     DYAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDC
                     LKAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDG
                     MGNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDL
                     LKGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPY
                     TIERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIEN
                     LII"
     gene            1077..1196
                     /locus_tag="IYI34_003107"
     CDS             1077..1196
                     /locus_tag="IYI34_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAR0560902.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
BASE COUNT          386 a          200 c          291 g          343 t
ORIGIN      
        1 ggtggcgtaa ccgcatcgac aaaaaccatg aggaaaattt ttgaccaact ggaaagtttc
       61 aatcccgagt tacctttaac gttactgttt gactatgtaa ggattcgctt tcccacaacg
      121 gatgcgagga aaatcattca agagattctc catttgaaat ttgattatat gctccacgaa
      181 gattacgcct tttactccta tcaagaacaa tatgttatgg gagatattgt ggtgatgttg
      241 tcacatgaag aagataaagg cgtcctttta gaattaaagg gtcgtggttg tcggcagttt
      301 gaaacttttt tactcgccca aaagcgtagc tggtatgact ttttcgaaga ttgtctaaaa
      361 gccggtggcg tgatgaaacg cttagacctg gcaatcaatg atcgagttgg cctgttagat
      421 attcctgatt taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttc
      481 aaaagctatc gttccgggga actgttgaaa cctgatgata aagatggtat gggcaatact
      541 ttgtatattg gcagtcttaa gagtgaggtc tatttctgtt tgtacgaaaa agactatgag
      601 caatatatca agttagggat tcctttagac cagaccgaaa cgaaaaatcg ttttgaaatc
      661 cgactgaaaa atgatcgagc ttatcatgca attcaagatt tgttaaaagg tcgcagtatt
      721 gaaagtacca cattttccat tattaaccgg tacttgcgat ttgcggataa ggtggaaggc
      781 aaaaggcgaa ctaactggcc cttgaatgaa caatgggggc gcttcattgg acggaatcag
      841 aaggaaattc aattaacttc tgaaccgaag ccttatacca ttgaacgaac gttgaactgg
      901 cttggacgac aagtcgcccc tacgtggaag atggccaagg agttggatcg gttgaatcag
      961 acaacgtaca ttcaggatat ggtaaataat actcgactat cagaccggca caaaaagatt
     1021 ttggagcaac aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtgatgg
     1081 aaattgtgaa agatatttta ttggtgtttg gtggcagtat gcttggtgtt actgtgatgt
     1141 gcttgatgca tgcaagtgca gcggcggacc gagcaatgga aaagaaagga aagtgatgaa
     1201 atgaatttcg gtcaaaattt
//
LOCUS       DACJGB010000194         1220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.206, whole genome shotgun sequence.
ACCESSION   DACJGB010000194 DACJGB010000000
VERSION     DACJGB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.206"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            49..1062
                     /locus_tag="IYI34_003108"
     CDS             49..1062
                     /locus_tag="IYI34_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAR0560903.1"
                     /translation="MESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHE
                     DYAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDC
                     LKAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDG
                     MGNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDL
                     LKGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPY
                     TIERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIEN
                     LIV"
     gene            1077..1172
                     /locus_tag="IYI34_003109"
     CDS             1077..1172
                     /locus_tag="IYI34_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAR0560904.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT          392 a          191 c          286 g          351 t
ORIGIN      
        1 ggtggcgtaa tcgcctctac aaagaccatg agaaaaattt ttgatcaact ggaaagtttc
       61 aatcccgatt tacctttaac gttactattt gactatgtaa ggattcgctt tcccacaacg
      121 gatgtgcgga aaattattca agaaattctc catttgaaat tcgagtatat gcttcatgaa
      181 gattacgcct tttactccta tcaagaacaa tatgtcatgg gcgatattgt agtgatgtta
      241 tctcatgaag aagataaagg tgtccttttg gaattaaaag gtcgtggttg ccggcagttt
      301 gaaacttttt tactcgccca aaagcgtagc tggtacgact tttttgaaga ttgtctaaaa
      361 gccggtggcg tgatgaaacg cttggattta gcaatcaatg atcgagtggg gctactggat
      421 attcctgatt taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttc
      481 aaaagttacc gttccgggga actgttgaaa gctgatgaaa aggatggtat gggcaatacc
      541 ttatatattg gcagcctcaa aagcgaggtt tatttttgtt tgtacgaaaa agattatgaa
      601 caatatatca agttagggat tccattggac cagaccgaaa cgaaaaatcg ttttgagatt
      661 cggttgaaaa atgaccgtgc ttattatgcg attcaagatt tgttaaaagg tcgtagcatt
      721 gaaagtacca cgttttccat tatcaatcgg tatttgcgat ttgcggataa agtagatgga
      781 aaacggcgaa ccaattggcc cttgaatgaa caatggggtc gttttattgg acggaatcgg
      841 aaagagattc aattaacttc tgaaccgaaa ccgtatacca ttgaacgaac attaaattgg
      901 ctaggtagac aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcaa
      961 acaacgtata tccaggatat ggtacagaat gcgcgactct cggaccggca taaaaagatt
     1021 ttggagcaac aaagtatggc aatcgaaaac ttgattgtat gaaaagagga attttgatgg
     1081 aaattgtaaa agatatgtta ttggtacttg gtggcagtat gcttggtgtt accgtgatgt
     1141 gcttgatgaa tgcaagtgca gcagcggatt gagcaatgga aaagaaagga aagtgacaaa
     1201 atgaatttcg gacaaaattt
//
LOCUS       DACJGB010000195         1215 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.207, whole genome shotgun sequence.
ACCESSION   DACJGB010000195 DACJGB010000000
VERSION     DACJGB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.207"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            414..947
                     /locus_tag="IYI34_003110"
     CDS             414..947
                     /locus_tag="IYI34_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAR0560905.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACJGB010000196         1195 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.208, whole genome shotgun sequence.
ACCESSION   DACJGB010000196 DACJGB010000000
VERSION     DACJGB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.208"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..1076
                     /locus_tag="IYI34_003111"
     CDS             <1..1076
                     /locus_tag="IYI34_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR0560906.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DACJGB010000197         1132 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.209, whole genome shotgun sequence.
ACCESSION   DACJGB010000197 DACJGB010000000
VERSION     DACJGB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.209"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            24..983
                     /locus_tag="IYI34_003112"
     CDS             24..983
                     /locus_tag="IYI34_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAR0560907.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          384 a          211 c          224 g          313 t
ORIGIN      
        1 taacggctct ttttttattg ccaattaacg gatttgccta tactatcaac aatgaattta
       61 atttgggcgc aaatgaaggt agctcacaag tagcaaataa tcagtatatt ttactgcatg
      121 aaacggctaa tgaaacagca acaggacgca atgaagcgca gtatatgcaa cgttcatgga
      181 ctagcgctta tactgcttat attgtgggag acggcggaat tgtttatcaa gtcggtcaac
      241 ctggttatgt acagtacggt gctggttcgt atgctaatgc caacagtcct gtgcagattg
      301 agttacaaca cacacatgat aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat
      361 tggctagaga ttcagcaatg aaatatggta ttccattaac gttggacact ccttataacc
      421 aaccgggaat caaatcgcat ttatgggtaa cacaaaacat ctggggcgat catacagatc
      481 cttacggtta tctttctgaa atgggcgtaa gtaaagaaaa attagcatat gatttagctc
      541 atggatttac cgatgaaaat ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac
      601 gagctggtgc agctaatcct acactgacag atggaacgaa ttacgcccac attgatcagt
      661 ttggagaaat cgaaaatgca aatttgcatg tagctggatg gcacattgct aactataaat
      721 acgagtatat cttcattatg gattacaata ctgggaaaga attagctcga gtaagagctg
      781 atggaattta tagagcagat gtaaatcaag cttataatac ttctggaaat gttggctatc
      841 atgtatcttt taacatgcgt aacttcccta ataagaaagt ctatgtcatg atgcgggcaa
      901 cgaatgatcc agagggaaac actaaaggcg gtgcgcaaga tttccatgac aaacgttggt
      961 atttaaatat tcctaaacga taaaaatagc tcctcgttga ggagcattac ataactatat
     1021 tgacaactat aaaaatcatt cgataaaata gtgatgttat cgcatatctt cactatcacc
     1081 cataatagtc acactccaag ctatgcgata acaggtttgt tgccacacat tc
//
LOCUS       DACJGB010000198         1127 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.210, whole genome shotgun sequence.
ACCESSION   DACJGB010000198 DACJGB010000000
VERSION     DACJGB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.210"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(178..251)
                     /locus_tag="IYI34_003113"
     tRNA            complement(178..251)
                     /locus_tag="IYI34_003113"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(215..217),aa:Ile,seq:cat)
     gene            complement(806..1120)
                     /locus_tag="IYI34_003114"
     CDS             complement(806..1120)
                     /locus_tag="IYI34_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560908.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          427 a          176 c          171 g          353 t
ORIGIN      
        1 aatcaaagta aaacttttca aatatacaga agtataacta tgtgaaacat cctttcatta
       61 atccataaaa ggatacataa aaaagccact catttgagtg gcaatgaaga aaagctttag
      121 cttgtataat actcttcaaa aaattctaac acagaacgat tcaattggct acattaatgt
      181 accctgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct gaccaactga
      241 gctaagggta gtggttgttg ccacataaag ccataaacaa tcaaccagta gaatgcgtgg
      301 caacaaacct gttatcgcat atcttggagt gtgactattt atgagtgata gtgaagatat
      361 gcgtctccat atatgtatgg ttgagacaga taaacatgta tgaaaaacgt tgttaaatca
      421 acgttttttt attttggtca aggagagaaa agtagagaaa tacaaaattt tttgcattga
      481 ttttgcattc aaaaaaacat tcaaacattt agaaatcccc aatacgtatt caggaaaatc
      541 tattttaagg tttacaaaaa gaacttttgt tcgtacactc tttttgaggt gaacttttat
      601 gctgatggaa ggaaaaacac aattatggtt taagcatgtt ccttcaaata agttttttaa
      661 ttataaactc tagagaaatt aattcatcct aacataaaat aattgttcac tcaaaagttt
      721 ttctagaaca attaaaacag caggcttcac attcgctata catttgtaag aagtaataat
      781 tcctgtacta aaaggcataa ttgctttaat tattttttga cttctcttca aacacaagaa
      841 gtagttgtgt aataattgca gaaataagga ttccaataat agatataaaa tttgacatgg
      901 aaacgtctat aacattaaaa gaataaagtg ttgaagaaag taaactgatt gaaataaccc
      961 ctccgtaaaa aatagtctgt gaatataccc actttttttc agaagacgta cttcgtttgg
     1021 ttctaaaacc tatccctgca tttctactct tatcaaaaaa ccaaatattt agaagtaata
     1081 atattgctat tattaagata aaattactgt aaaaatccaa aagctat
//
LOCUS       DACJGB010000199         1069 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.211, whole genome shotgun sequence.
ACCESSION   DACJGB010000199 DACJGB010000000
VERSION     DACJGB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.211"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            37..588
                     /locus_tag="IYI34_003115"
     CDS             37..588
                     /locus_tag="IYI34_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HAR0560909.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
BASE COUNT          412 a          144 c          204 g          309 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa atggttctgt tgcaaagttt tccaaaaaat ctattttagt
     1021 gtaaaattga gaaaaaagac agagaggaca gagtaatgaa tcattttaa
//
LOCUS       DACJGB010000200         1062 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.212, whole genome shotgun sequence.
ACCESSION   DACJGB010000200 DACJGB010000000
VERSION     DACJGB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.212"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(37..993)
                     /locus_tag="IYI34_003116"
     CDS             complement(37..993)
                     /locus_tag="IYI34_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560910.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DACJGB010000201         1048 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.213, whole genome shotgun sequence.
ACCESSION   DACJGB010000201 DACJGB010000000
VERSION     DACJGB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.213"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            12..506
                     /locus_tag="IYI34_003117"
     CDS             12..506
                     /locus_tag="IYI34_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560911.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            520..951
                     /locus_tag="IYI34_003118"
     CDS             520..951
                     /locus_tag="IYI34_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560912.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            953..>1048
                     /locus_tag="IYI34_003119"
     CDS             953..>1048
                     /locus_tag="IYI34_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAR0560913.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEE"
BASE COUNT          388 a          150 c          227 g          283 t
ORIGIN      
        1 gggacggttt aatggctgat attgtgcaat taaaagaaga cggagttgct aaatacttaa
       61 aaacgcatgt agaagctatt gatggtaaag aagcattggt gcagacagat ggagaccaag
      121 ccattgcagg acataagaat ttttcaggtt ctgtaacaat caataataaa cgtgttttga
      181 cgactgatga taacagatat gaagtggtaa atctggttgt cactaacggt aatacaggga
      241 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
      301 aaagtagtgg cgggaatgat tcggttattt taactgttcc agaaggctat cggacaccaa
      361 ttagttttga acaactggtt gggtcaatag accgttctac tttgaacagt gctcaactat
      421 ctattggcgc agacggagcc attaaatgga gaagaaattc aagttatgga tcagcttatt
      481 catttgttat cacttattca atttaaggga ggaaatctaa tgaaagtagt ttacaaatca
      541 atcaagcctt acggattcga gcaaatcatt ttgaacaatc aagaaaatat ccctgaaaac
      601 tgtacagaga ttaaaccacc agttcctaac tggagaccaa gatttgattt tgataaaaaa
      661 cagtgggttg agttagctac tgaagaagag aaaaaaggga atgctgttga tagcgtagat
      721 gaattggcta atattaaggc attatacgaa acgctgaagg cagaaaatga tgaattgaaa
      781 cagctaaatt ctaaagcaat gctaaacaat gtagcaatca agcaagaaaa tgtcttattg
      841 aaagaaaagt cagaaagttt agcgcagttg aattcaaaaa cgatgcttgc ttctgtacaa
      901 aataccaagg aaatcgaaga aatcaaaaaa caattacaag gtgggaagta acatgtattc
      961 atatgaagat atcaaactaa tgtatgactg gggctgtttc actaacgaac aagtaatggt
     1021 tttcgttccg ttgtgcatta ctgaagaa
//
LOCUS       DACJGB010000202         1045 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.214, whole genome shotgun sequence.
ACCESSION   DACJGB010000202 DACJGB010000000
VERSION     DACJGB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.214"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            6..980
                     /locus_tag="IYI34_003120"
     CDS             6..980
                     /locus_tag="IYI34_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAR0560914.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DACJGB010000203         1035 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.215, whole genome shotgun sequence.
ACCESSION   DACJGB010000203 DACJGB010000000
VERSION     DACJGB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.215"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..>1035)
                     /locus_tag="IYI34_003121"
     rRNA            complement(<1..>1035)
                     /locus_tag="IYI34_003121"
                     /product="16S ribosomal RNA"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DACJGB010000204         1024 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.216, whole genome shotgun sequence.
ACCESSION   DACJGB010000204 DACJGB010000000
VERSION     DACJGB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.216"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..292
                     /locus_tag="IYI34_003122"
     CDS             <1..292
                     /locus_tag="IYI34_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAR0560915.1"
                     /translation="QKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFKKQI
                     KKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT          368 a          182 c          132 g          342 t
ORIGIN      
        1 gcaaaaaact tatcctaaag tgacgcagtc actgataaaa aatcaagatt tattgacttt
       61 ttatgagttt ccgcctggta tccgccgaag catttactca actaatttaa tcgaatcttt
      121 caagaaacaa atcaagaaat acagccgcag aaaagagcag tttcaaaatg aagaatcact
      181 agagcgtttc ctagtatcca tctttgatac atacaatcaa aaattcttaa atagaagcca
      241 taaaggcttc caacaagtga cggatacatt agcttcaatg tttactgagt aacccattat
      301 tttgcagaag gacgagttat ttacacaaaa ttattgacgc tccctccaaa ttacttcatc
      361 aaaacattga tataattttt attatatcag tatttattct tgatacataa atgagaaaat
      421 aaatacttta aatctattga ctacaagtag tcattaagtc ttcataagat gtacaatatg
      481 acattaaaaa aataaaagct tacgaccatc cgttaaatct gacactaaat aatggactgc
      541 tttcttttgt attagcaggt tacgttgact atgaatattc accagattat ttggagaaac
      601 ctcttcaaca cgagaacaat gatattaatt ttagatttgt ctacgagtca aaaagtaaaa
      661 catggttagt tgatcaatta agcacgataa acacaagctt ccatgaatta cctaaacctg
      721 aaaatgaagt tgagatggtc attaaaatct tctaaaaaag aagcgttgaa ggggaaatat
      781 tcctttcaac gctctttttt atatgcttag aaacataaat aaattgtata ctttcttttt
      841 agcccctatt acttttcacg aaaaatatct ttttttcgac gctagcgttt ctctacttct
      901 aatactgtac acagtttttg tattattgaa aaattactca ttatttagca ttaatccaca
      961 tcgttttatc aatcgacctt catactaaac tactttaaag tcatcttcct tctcgttttt
     1021 catt
//
LOCUS       DACJGB010000205         1003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.217, whole genome shotgun sequence.
ACCESSION   DACJGB010000205 DACJGB010000000
VERSION     DACJGB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.217"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..>1003
                     /locus_tag="IYI34_003123"
     CDS             <1..>1003
                     /locus_tag="IYI34_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR0560916.1"
                     /translation="GKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLVTP
                     FCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPRCL
                     LFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHYIV
                     TDMNAPYFSLVKECFPNAQVIVDRFHIVQHLNRFFDAVRKRVMKTLDQKDPIQAKQYR
                     QLKSLFKLLLKSEDRLDYNTLTKRRNFNWRLLTETEVVDRLLKISPELKTAYRYYQDL
                     LTAYQEKDPDTFFQLLRAMPGEVPLELHHIKKAFFKYEKGIRLALLKKYSNARLENLH
                     "
BASE COUNT          300 a          209 c          191 g          303 t
ORIGIN      
        1 tggcaagaag atctctatga ttctcctgaa tcgtagcggt aataaacgga cctatcttcg
       61 gttgaagaaa cagcgatacc tctgccgagc atgtaaaaaa tattttaccg ctcggacgta
      121 tctcgttacc ccattctgct ttatttctaa gcagattcat tacaagatct tagaagaatt
      181 gacagagcgt caatctataa aagcaattgg taaacattgc gatgtttctg tgacaactgt
      241 tcagcggaca ttgtccgcct tgtcatcaga tatccgtcag aatcttgagt atttacctcg
      301 gtgtctatta ttcgatgagt ttcgttctct ttctacatgg cacggtaaat tttctttttc
      361 ttgtatggac ggtgagaccg ggaaactgtt tgagatctta cctagccgcc gtaaaaaaga
      421 tctcgttgct tttttcatgc gtttttctct caaagcgagg ttgggtgtac attacatcgt
      481 aacggatatg aacgctcctt atttctcact agttaaagag tgttttccaa atgcacaagt
      541 gatcgtcgat cggtttcaca ttgttcaaca tctgaaccgc ttcttcgatg cggttcgaaa
      601 acgtgtgatg aaaacacttg atcaaaaaga cccaatacaa gctaaacagt atcgccaatt
      661 gaaatcctta ttcaaattac ttttaaaatc agaggatcgt ttagattaca atacactaac
      721 gaaaagacgc aatttcaatt ggagactatt gactgaaaca gaagtagttg accgccttct
      781 aaagatttct cctgaactta aaacagctta tcgttactac caagacctgc ttactgcgta
      841 tcaggaaaaa gatccagata cttttttcca gctgcttcga gcaatgcctg gagaagttcc
      901 attggaactg caccatatca aaaaagcctt tttcaaatac gagaaaggga ttcggctggc
      961 attactgaag aagtattcga atgcacgact ggaaaatctc cat
//
LOCUS       DACJGB010000206         1003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.218, whole genome shotgun sequence.
ACCESSION   DACJGB010000206 DACJGB010000000
VERSION     DACJGB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.218"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
BASE COUNT          379 a          121 c          137 g          366 t
ORIGIN      
        1 tttcggtgtc tactgaaatc aaggtattaa tgggaataac agcctaagat atttggagtt
       61 cagagagggc gcgtttgatt ttcaaacttc gcaacagaac ccattagcaa tatataaaag
      121 aaggtatcaa gagtcacagt gaatttattc cgaaaagtga aaagagcaaa tataaaattt
      181 aataaaaata agaactaaat aggaaaacgt agacgtaatt ttctacgttt tccttttcat
      241 atatattaaa tattacggaa aattatataa actaatttag tgttttgttt gtaattccta
      301 attttgctcc acttttagat aatttttagg tctatttaac tcattagaca ttctatgctc
      361 atttttctcc aaatttgttt caactctttc gtacttaatt ttgcgtattt gaaaattctt
      421 cttttagagt acttttatta cgatactagt atgatatata tctacagcaa acagttaaaa
      481 tcttcctaaa ataagcgaat agcattccta aaacctataa aatttttaga tgaagcgaca
      541 agtggacaat taataaacta cttgaacatg aaattatgga tcacctttca aatttagaaa
      601 atggaattct tatacttatt gcgcaacatt tattgattgc aaaattatgt gacaaaaaga
      661 actagtagaa gaaggaataa tggcttgata ataaagtact acctattaaa ggtagtactt
      721 tatttaattt agtataaatt aacatactac tatattttag gtttcatatt atataaatta
      781 ataatatact accatttgtt ggtagtataa tttaaaggta ataagttatt cttttattac
      841 tatgtttggg ttttatagca aagaaagttt aataagagtt tggacctagc aaacaatatt
      901 aatgaggtca ttaaatctta ttatctttag tgatataatg aaatatctac acgacaaaat
      961 tatgatcttg cgtaaagtta taaaatatta ataaaaatat ttt
//
LOCUS       DACJGB010000207         1000 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.219, whole genome shotgun sequence.
ACCESSION   DACJGB010000207 DACJGB010000000
VERSION     DACJGB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.219"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            369..1000
                     /locus_tag="IYI34_003124"
                     /pseudo
     CDS             369..1000
                     /locus_tag="IYI34_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          294 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac
//
LOCUS       DACJGB010000208          998 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.220, whole genome shotgun sequence.
ACCESSION   DACJGB010000208 DACJGB010000000
VERSION     DACJGB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.220"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(80..634)
                     /locus_tag="IYI34_003125"
     CDS             complement(80..634)
                     /locus_tag="IYI34_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR0560917.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DACJGB010000209          980 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.221, whole genome shotgun sequence.
ACCESSION   DACJGB010000209 DACJGB010000000
VERSION     DACJGB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.221"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            2..>980
                     /locus_tag="IYI34_003126"
     rRNA            2..>980
                     /locus_tag="IYI34_003126"
                     /product="23S ribosomal RNA"
BASE COUNT          304 a          194 c          284 g          198 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg
//
LOCUS       DACJGB010000210          976 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.222, whole genome shotgun sequence.
ACCESSION   DACJGB010000210 DACJGB010000000
VERSION     DACJGB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.222"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            82..>976
                     /locus_tag="IYI34_003127"
     CDS             82..>976
                     /locus_tag="IYI34_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAR0560918.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHY"
BASE COUNT          331 a          182 c          192 g          271 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatg
//
LOCUS       DACJGB010000211          973 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.223, whole genome shotgun sequence.
ACCESSION   DACJGB010000211 DACJGB010000000
VERSION     DACJGB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.223"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            227..568
                     /locus_tag="IYI34_003128"
     CDS             227..568
                     /locus_tag="IYI34_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560919.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETNNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLWKQPRKPEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            561..>973
                     /locus_tag="IYI34_003129"
     CDS             561..>973
                     /locus_tag="IYI34_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAR0560920.1"
                     /translation="MSEGTKRNDNKLFNSLYKITVNDVVEKRNKLTYLSWAWAWAEVS
                     KICDEVDYEIYRDPETNRPYLFDEKTGYMVFTSITVNGVKRDMWLPVMDGANKAMKDE
                     PYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFD"
BASE COUNT          375 a          160 c          210 g          228 t
ORIGIN      
        1 gatcaactga acagacaaca aagacttttc ggtgatgaaa atgaatagag ctgaagctct
       61 aaaaatcggt aaagtgatgg ctgataattg gtgggcaaat agccgtccta ttattttaag
      121 caagcaacat attgataagc aaaaagcatg gcaacaaata aaaaagtgac tcagccgacc
      181 aaagcaatga gtcacaaaga aaatacatct aaggagatgt taccacatga aaaaagaact
      241 ttccactcta gatcaatatt tgactgatcc tagttggggc aaatcgaata tcaaggaaac
      301 aaacaaccga aaaatcagac gaaatctttt gacagatgaa gaactagcat gtgatcaaga
      361 cgatttgggc aattttgtga gtatttgggg acatgtctac cttatccatc tatggaagca
      421 gccaagaaaa cctgaataca tctatgtcat cgaagatggc ttgattgatg cactagaaga
      481 gtacgacaga gataacttga ttgatatctc ttattacgga tcaggtaaga aatacattgc
      541 tgaaatggag gcagaatttg atgagtgaag gaacgaaacg caacgataac aaattattca
      601 atagtctgta caagataacc gtcaatgatg ttgtcgaaaa aagaaacaaa ctaacttatc
      661 tgtcctgggc atgggcgtgg gcagaagtca gcaaaatctg cgatgaagta gactacgaaa
      721 tctatcgtga tccagaaaca aatcgtccat acctctttga tgaaaaaaca ggctatatgg
      781 tttttaccag tatcacagtc aacggagtaa agcgtgacat gtggttacca gtcatggatg
      841 gtgcaaacaa ggcaatgaaa gatgagccat atacctacga agtcaatgat tatcagtgga
      901 ataacgaaac gaagaaaaaa gagattgttg gaaaaatcga aaagcgagtt gaagcagcaa
      961 ctatgtttga tat
//
LOCUS       DACJGB010000212          970 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.224, whole genome shotgun sequence.
ACCESSION   DACJGB010000212 DACJGB010000000
VERSION     DACJGB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.224"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            65..>970
                     /locus_tag="IYI34_003130"
     CDS             65..>970
                     /locus_tag="IYI34_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAR0560921.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKG
                     ITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFES
                     ILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          346 a          172 c          168 g          284 t
ORIGIN      
        1 gttggtaaat caacaaaaaa acgcatatag tagtctcaaa actcccaaga aatgaggact
       61 acatatgcgt tcatcaaaat atactgaaca ttatacagat acttttataa cattcaagga
      121 aattatgaga actgtttcta acatatacca caattgtgta ccagataaga ttaaaaaccg
      181 aagaaatact gatcaactga agcaacgcga tacagtgatt attgcttgtg ttatatgggg
      241 cataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt ccgtcttatt
      301 tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa acttagctta
      361 cactatcaag attattcgat actttttcat caagaagctc accaaaggtg aactagtcgg
      421 aattatagat agttttccta gtccattatg taaaccagtt agaaatagac aagcaaaact
      481 attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt tttatggtct
      541 taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa tcacgaatcc
      601 aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc ttcccaatat
      661 cctaggggac aaaggatata ttagccataa aatccatgaa aagcttgccc ttaagggcat
      721 tactatatcc gttccgccac gtaaaaacat ggataaatca gaaaaactag accacagctt
      781 acttggaaaa caaagaaaaa cagttgagac tgttttttct tccttagaaa aattaggttg
      841 tcaaaatttc aactcacgtt ctgttaaagg gctagagagt agatttgaaa gcatattact
      901 ggcttacagt gttctattaa gtcgagcaca acgacgtttt gaaggaactt tgagatattc
      961 tttaggatat
//
LOCUS       DACJGB010000213          864 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.225, whole genome shotgun sequence.
ACCESSION   DACJGB010000213 DACJGB010000000
VERSION     DACJGB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.225"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..434)
                     /locus_tag="IYI34_003131"
     CDS             complement(<1..434)
                     /locus_tag="IYI34_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAR0560922.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /locus_tag="IYI34_003132"
     CDS             complement(451..855)
                     /locus_tag="IYI34_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAR0560923.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          261 a          140 c          136 g          327 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atca
//
LOCUS       DACJGB010000214          842 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.226, whole genome shotgun sequence.
ACCESSION   DACJGB010000214 DACJGB010000000
VERSION     DACJGB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.226"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..>842
                     /locus_tag="IYI34_003133"
     CDS             <1..>842
                     /locus_tag="IYI34_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAR0560924.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLK"
BASE COUNT          367 a          108 c          136 g          231 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 tt
//
LOCUS       DACJGB010000215          838 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.227, whole genome shotgun sequence.
ACCESSION   DACJGB010000215 DACJGB010000000
VERSION     DACJGB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.227"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..284)
                     /locus_tag="IYI34_003134"
     CDS             complement(<1..284)
                     /locus_tag="IYI34_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0560925.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVA"
     gene            complement(431..>838)
                     /locus_tag="IYI34_003135"
     CDS             complement(431..>838)
                     /locus_tag="IYI34_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAR0560926.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          270 a          163 c          138 g          267 t
ORIGIN      
        1 taagcgacat tggtatcgac ttccttaatg gtttgtcgca tggaacggat accgaataaa
       61 tactgaataa acaccaattt gatcagaatc actggatcga tacttgggcg acctaaatcg
      121 gagtaagtgg cttcgacgat tggataaata aattcaaaat caagcgcggc atcaatcttt
      181 cgaacgagat gatcttgagg aactagatca tcaatcgtaa gcatcgcaaa ttgatttcta
      241 ccattcgtat tgtttttaga catcatagct atcactcctt gcattcgttt tcataaatct
      301 attataaaag aaaagactgt agatatcatc cataaaatgg actttgtcta cagtctgaga
      361 cacgacctaa agatcgtgtc taattatgac tgtatcagaa aatatgatac caaacggatc
      421 tattcattaa ttaattgaca tagtttatct gttgctcttt tatgactgga taactgacca
      481 attcatgacg atccaagtct tctttattca tatttacagc agtaatttga ctgtcttcat
      541 acgtacgata ataataaatc cctttatcaa cgttacagca agaagaataa atcgtatatt
      601 cgtattttcc ttctccagta tcgcatactc ctttttgttg ctcgactgag cctagtatat
      661 gaaaaaattg actgatactt tcagattctg aatcacctga tgcagcattc attttagtaa
      721 atgtggcttt tacaaatcga gaaacagaag agagatctcc aggtaagccc aatcctccca
      781 tcccacgact ataaacattc aagctgatct ggtttgaaaa atgattttct ggtgttcc
//
LOCUS       DACJGB010000216          814 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.228, whole genome shotgun sequence.
ACCESSION   DACJGB010000216 DACJGB010000000
VERSION     DACJGB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.228"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(25..630)
                     /locus_tag="IYI34_003136"
     CDS             complement(25..630)
                     /locus_tag="IYI34_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAR0560927.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          251 a          144 c          122 g          297 t
ORIGIN      
        1 aaagcgaatg aatgtagtat atttctacaa atcattcgct ttctttattt tataaatagt
       61 tggtcgagag atcccgtttt cttttgcaat ctctgcaact ggaatgcctt gctttaacat
      121 gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa tagaatactc
      181 gattggtcgt cccttatatc ggccggcttt ttgagccaat aatactcctt gccgctgacg
      241 ttcacgaatt ttctcacgct cgttttgtgc aatataactt agcagtgata aaaacatatc
      301 gaacatagcg gtatccagga gttcatttcc agtattaaaa tctaaaaact gtgcatctaa
      361 tatttttaac gctacttttt tctgtttcat aaaagcaact gtatttttta tatcttcata
      421 atctcttcct aaacgatcta gtgatgtaac gatgacgcaa tcaccttcac ggatataatt
      481 tagtaatttt tgaaactcag gacgctcagt atctttacca ctggcttttt cttggaataa
      541 tttatcaatg ttaagtcctt ccatcattgc caattgtcga tccaaatttt gatcttgtga
      601 agatactcga atatagccaa tttttcccat aaaaaactcc tttttaaaag tgatataatt
      661 tacatgtaaa atttacttgt agttttactg ctatcatagc atttttaaaa ataaatttta
      721 tgtagactta aaatttactt catggagggt ttaaatggat gaaatcaaaa gtaacaaaaa
      781 agcatttggc atttatttag gagaacctag taca
//
LOCUS       DACJGB010000217          804 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.229, whole genome shotgun sequence.
ACCESSION   DACJGB010000217 DACJGB010000000
VERSION     DACJGB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.229"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..693
                     /gene="tnpB"
                     /locus_tag="IYI34_003137"
     CDS             <1..693
                     /gene="tnpB"
                     /locus_tag="IYI34_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAR0560928.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          126 c          148 g          234 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct ccca
//
LOCUS       DACJGB010000218          800 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.230, whole genome shotgun sequence.
ACCESSION   DACJGB010000218 DACJGB010000000
VERSION     DACJGB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.230"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(324..524)
                     /locus_tag="IYI34_003138"
     CDS             complement(324..524)
                     /locus_tag="IYI34_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAR0560929.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          287 a          125 c          127 g          261 t
ORIGIN      
        1 tctttttcgt tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata
       61 aattcttcca tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa
      121 aggtggaatt tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc
      181 aaaaaaaatc gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct
      241 tataataaca aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc
      301 tgtatccttt gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg
      361 ctatgtccta aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat
      421 gcatgcgtat gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt
      481 actaaagaaa aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga
      541 attaaacgat ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa
      601 ctctagcggt ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt
      661 tttattattt ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc
      721 atataaaaaa acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc
      781 ctttattcta tgctttatat
//
LOCUS       DACJGB010000219          783 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.231, whole genome shotgun sequence.
ACCESSION   DACJGB010000219 DACJGB010000000
VERSION     DACJGB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.231"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            24..716
                     /locus_tag="IYI34_003139"
     CDS             24..716
                     /locus_tag="IYI34_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAR0560930.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATVATTTTTSTESKSTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNADKSAQLEAF
                     MTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSESGTGQA
                     DYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELK
                     NGFNQIVYGQAN"
BASE COUNT          281 a          151 c          159 g          192 t
ORIGIN      
        1 tgaaaaaaag gattttagta acaattggtc tagctttttt gttagctggt tgtaccaacg
       61 ggaatgaatc gaaaaaagaa tcaacaaaag aaacagcgaa agcaacagtt gcaactacta
      121 caacaactag cacagagagc aagtcaactg cagcaagtac agaaacagca gatacctcta
      181 cttcgtcaga gtcttccaaa acgactgaaa caactcaaac atctacttca agttcttctg
      241 tagaggcttc atcttcctct tcagaaagca gcgaacaagt tgaacaaaca ctgtggaatg
      301 cagacaaatc cgcacaatta gaggcattta tgaccaaatg gggccaaaca atgggacaac
      361 aatacaaaag ttatacgaat caaatgagtg tggacttata tggtcttaaa gtccctcaag
      421 ctattttgaa cggggagtgg aagatggcta ttggtggagt gcctgtttct gcagaatggt
      481 cagaaagtgg gacaggtcaa gcagactatc aaatcacagc cgtctattcc gatgcagaga
      541 cagaaccata tctgaaaaag cacgtctatc tattcgggtt tcagcaaaat cagccaaaag
      601 tattggtgac acaacaaaat caggggaatc ccgataatta tctttacttc aacgagacag
      661 cgaataatga attgaaaaat ggctttaatc aaatcgttta tggacaagca aattaatgag
      721 ataaacaagg ggttgtgaca tgtcttttat cacaacccct tgctcagttt atttgttagt
      781 ctt
//
LOCUS       DACJGB010000220          729 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.234, whole genome shotgun sequence.
ACCESSION   DACJGB010000220 DACJGB010000000
VERSION     DACJGB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.234"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..>729)
                     /locus_tag="IYI34_003140"
     CDS             complement(<1..>729)
                     /locus_tag="IYI34_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0560931.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCTKSKNHQRVITRHVWGDLMDEVEHLRLTDLNKS
                     IYKKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKE
                     PLFFCSKIRNEVDKKLFQARVTSL"
BASE COUNT          202 a          148 c          134 g          245 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat gactcgttga tgatttttac ttttggtaca ttggtttagc
      361 agaggacagt tcacacattc ttttggattg gatttgtatt ctcggtacct ttctcgagta
      421 accgttgaaa aagcgagcat ttgatcttgc ggacaaatat aacaatcaaa atactcgtca
      481 tagagaaact cattttttga taatagcccc tttttccctt tgggccttga ataaggcaaa
      541 caaggtcgaa ggtttaaatg agtcaaaaag tgaacaaact taggtgtctt atactcagca
      601 tcagcgacaa cacaattgat ttcaggaaaa agtctcttcg attgtttgat gagctgaaag
      661 gcgacttgac tgtcatgaat atttctagga gtgatgattg tgctaagaat aaatccattg
      721 tcgtcgcat
//
LOCUS       DACJGB010000221          649 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.235, whole genome shotgun sequence.
ACCESSION   DACJGB010000221 DACJGB010000000
VERSION     DACJGB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.235"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..>649
                     /locus_tag="IYI34_003141"
     CDS             <1..>649
                     /locus_tag="IYI34_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAR0560932.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DACJGB010000222          569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.236, whole genome shotgun sequence.
ACCESSION   DACJGB010000222 DACJGB010000000
VERSION     DACJGB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.236"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
BASE COUNT          185 a           81 c           81 g          222 t
ORIGIN      
        1 tcttgatgtg atatccttgt cttttttgta atgtttttaa cttattgctt ttgcaaaatt
       61 tatttacctg ttttctggat caacgatagt gtacattatg ctatccttgt ttggatttta
      121 ggacgtttaa agaggcactt ttttacataa cttaatccat agacctttta agcatgctgt
      181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtatta
      241 cgctttaaac tcttttatat ttgaattcac taagaagcat accatatttt tgaaaaaaag
      301 tgagaaaaaa ggcttataat taggatttag agtaattaat tagatgttta ttttatagga
      361 ctgatactat agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg
      421 tgaataagat aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac
      481 ctttctggat caacgaaagt atactttata tagtcataac gccgatggta actatgcact
      541 gtaacgataa tgctgctaaa atatctttt
//
LOCUS       DACJGB010000223          559 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.237, whole genome shotgun sequence.
ACCESSION   DACJGB010000223 DACJGB010000000
VERSION     DACJGB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.237"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            75..>559
                     /locus_tag="IYI34_003142"
     CDS             75..>559
                     /locus_tag="IYI34_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAR0560933.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          209 a          103 c           90 g          157 t
ORIGIN      
        1 tccatacaaa aaaggaagtc ccttctgtag aataaagtta acgacaacca attcacagaa
       61 aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag
      121 gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga
      181 ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta
      241 attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg
      301 aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa
      361 caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg
      421 aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca
      481 acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata
      541 agtatgtcgc tatttttat
//
LOCUS       DACJGB010000224          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.238, whole genome shotgun sequence.
ACCESSION   DACJGB010000224 DACJGB010000000
VERSION     DACJGB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.238"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(5..90)
                     /locus_tag="IYI34_003143"
     tRNA            complement(5..90)
                     /locus_tag="IYI34_003143"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IYI34_003144"
     tRNA            complement(107..178)
                     /locus_tag="IYI34_003144"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IYI34_003145"
     tRNA            complement(190..262)
                     /locus_tag="IYI34_003145"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IYI34_003146"
     tRNA            complement(280..361)
                     /locus_tag="IYI34_003146"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IYI34_003147"
     tRNA            complement(369..441)
                     /locus_tag="IYI34_003147"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IYI34_003148"
     tRNA            complement(445..517)
                     /locus_tag="IYI34_003148"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACJGB010000225          524 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.239, whole genome shotgun sequence.
ACCESSION   DACJGB010000225 DACJGB010000000
VERSION     DACJGB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.239"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..>524
                     /locus_tag="IYI34_003149"
     CDS             <1..>524
                     /locus_tag="IYI34_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAR0560934.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPA"
BASE COUNT          155 a           73 c          120 g          176 t
ORIGIN      
        1 ttattttaag tctgaaaagg atacatcgtc tctaacgtta actttagagg aaggagttga
       61 acctgacaag gtgacctcta aagtgataaa ccaattgaaa gaaaaaggta gtttgaaaca
      121 tttgggggaa tatgaagtgt tagatacggc tatgttaacg aaaggaattg gtcaaatcct
      181 cagtacgatc acttatttta taacagctgt agcaggtata tctttattta tcgcaggggt
      241 aggtgtaatg aacatgatgt atatatctgt atctgaacga acaaaagaga tcggtataag
      301 aagagcccta ggagctactc gaaaatcgat tatgctgcag ttccttttag aaggtttgat
      361 tttaactctt tcaggagggt taattggcta tttattagga atggctcttg cttatagtgt
      421 tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac tagcggttgg
      481 ggtatcttcg gttattggac tggtcttttc tgtaatgccc gctt
//
LOCUS       DACJGB010000226          509 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.240, whole genome shotgun sequence.
ACCESSION   DACJGB010000226 DACJGB010000000
VERSION     DACJGB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.240"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..132)
                     /locus_tag="IYI34_003150"
     CDS             complement(<1..132)
                     /locus_tag="IYI34_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR0560935.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACJGB010000227          503 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.241, whole genome shotgun sequence.
ACCESSION   DACJGB010000227 DACJGB010000000
VERSION     DACJGB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.241"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            10..375
                     /locus_tag="IYI34_003151"
     CDS             10..375
                     /locus_tag="IYI34_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0560936.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
BASE COUNT          201 a           35 c           86 g          181 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac att
//
LOCUS       DACJGB010000228          496 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.242, whole genome shotgun sequence.
ACCESSION   DACJGB010000228 DACJGB010000000
VERSION     DACJGB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.242"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..133
                     /locus_tag="IYI34_003152"
     CDS             <1..133
                     /locus_tag="IYI34_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAR0560937.1"
                     /translation="AATYYGNGLYCNKEKCWVNWGQSWSEGLKRWGDNLFGSFSGGR"
     gene            133..483
                     /locus_tag="IYI34_003153"
     CDS             133..483
                     /locus_tag="IYI34_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAR0560938.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          200 a           55 c           83 g          158 t
ORIGIN      
        1 cgcagctact tactatggaa atggtcttta ttgtaacaaa gaaaaatgtt gggtaaattg
       61 gggacaatca tggtcagaag ggttaaaacg ttggggagat aatctttttg gtagttttag
      121 tggaggacga taatgacttc aataaacatt gatcacacaa aaaaagtcat acatgaatta
      181 tacaatagtt taatgacacg acctaatcag acgaaagcat tattagatat tactgatgtt
      241 ttatttcagg tatataaaaa aattgataca gcaaaatatc ctgaaattct tgtaaataaa
      301 ttagttaatt atatttacat cgtgggtttt gataataaaa tacgctttat tgataatgat
      361 gaaaaattat taatagaatt aggggatatt tctaaaaaag ctggaattaa tagtaaatac
      421 aaagcaaact ttacagataa atcgcaattt tatagttatt cagaaacaat gcctcaacga
      481 taaaatatag tatact
//
LOCUS       DACJGB010000229          493 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.243, whole genome shotgun sequence.
ACCESSION   DACJGB010000229 DACJGB010000000
VERSION     DACJGB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.243"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(54..>493)
                     /locus_tag="IYI34_003154"
     CDS             complement(54..>493)
                     /locus_tag="IYI34_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAR0560939.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          133 a           92 c          115 g          153 t
ORIGIN      
        1 tcgtaaaaaa ttttcgatct gggcaaagaa gtggtatact gtaagtggtt tagttaatgg
       61 tccactgaga ccgcgtacgc atagtcgtac tctgtatgtg ccctaaccat agcgtgcata
      121 catttcagaa gcttgttcat acaagcaact gtggcaacct tatctttctt agggataggt
      181 tgctttttta atttgtagta ataatcgaca atatgattag gagctgcgcg ttgctggcga
      241 atcatatttc gaatggtgaa gaaaataatt ttccgtgctt tgggattacc acgcttattg
      301 atgcggtctt ggccagtata cttacctgaa tgataccgtc ggatgtcgat tccgacaaag
      361 gcattcaatt gtttatgggt tttaaatcgt gtgatatctc ctaattcacc gagcaataaa
      421 gcggctgtga gtgcgccaat cccagggaac gtttggtaca acaaaaagca aggagagcct
      481 tcggcttttt tga
//
LOCUS       DACJGB010000230          493 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.244, whole genome shotgun sequence.
ACCESSION   DACJGB010000230 DACJGB010000000
VERSION     DACJGB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.244"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            411..>493
                     /locus_tag="IYI34_003155"
     CDS             411..>493
                     /locus_tag="IYI34_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAR0560940.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRV"
BASE COUNT          159 a           84 c           86 g          164 t
ORIGIN      
        1 tttatcacgg atgagcttga aatcgtcttt tatacgtatc aaattgaaat gttcggcata
       61 caacctatcg ttgttactac attactaatt gtcacttgtt aaggaacttg gatcctttct
      121 aaacattccc tagagataaa ttttacttta tccctccgtt aatatccaat ttaagcacct
      181 ttccgattca gggttaattt gcatatcaag tgcagcttgt gttctagtaa gattagccct
      241 tttagtccgg ctgagttgac ttatggatat aagatatggt catagcaaat agatactacg
      301 tatagtctgt taatttagtg tcattaatct taagttggat aggctacaat taactagaca
      361 gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg aggaattttt atgtctaaga
      421 gaacacgaag aactttttca caagaattca agcaacaaat cgtcaatctt tacttagctg
      481 gaaagccacg tgt
//
LOCUS       DACJGB010000231          470 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.245, whole genome shotgun sequence.
ACCESSION   DACJGB010000231 DACJGB010000000
VERSION     DACJGB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.245"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..>470
                     /locus_tag="IYI34_003156"
     CDS             <1..>470
                     /locus_tag="IYI34_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAR0560941.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DACJGB010000232          468 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.246, whole genome shotgun sequence.
ACCESSION   DACJGB010000232 DACJGB010000000
VERSION     DACJGB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.246"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..240
                     /locus_tag="IYI34_003157"
     CDS             <1..240
                     /locus_tag="IYI34_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAR0560942.1"
                     /translation="SDILEVLQKVYLKLEKQKYELDPSPLINRLVNYLYFTAYTNKIR
                     FTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            344..>468
                     /locus_tag="IYI34_003158"
     CDS             344..>468
                     /locus_tag="IYI34_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAR0560943.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
BASE COUNT          194 a           44 c           81 g          149 t
ORIGIN      
        1 tctgatattc ttgaggtttt acaaaaggta tatttaaaat tagaaaaaca aaaatatgaa
       61 ttagatccca gtcctttaat aaatagattg gtgaattatc tatattttac tgcttatact
      121 aataaaataa gattcactga atatcaagag gaattaataa gaaatttgag tgaaattgga
      181 agaactgctg gaataaatgg tttatatcga gcagattatg gagataaatc tcaattttaa
      241 tgatacttat aatattaaat atgtctcaaa attttttgat tagaaaaata ggtatattga
      301 atcagtgaca taaaagtatt tatagtttgt agggggaatc gtaatggaag aaaaaaatag
      361 actaaatgct aaacaatgtt cagatcaaga gcttaaaaaa attaaaggag gtgctggaac
      421 aaaacctcaa ggaaaaccgg catcaaattt agtggaatgt gttttttc
//
LOCUS       DACJGB010000233          462 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.247, whole genome shotgun sequence.
ACCESSION   DACJGB010000233 DACJGB010000000
VERSION     DACJGB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.247"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(<1..430)
                     /locus_tag="IYI34_003159"
     CDS             complement(<1..430)
                     /locus_tag="IYI34_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAR0560944.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DACJGB010000234          460 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.denovo.248, whole genome shotgun sequence.
ACCESSION   DACJGB010000234 DACJGB010000000
VERSION     DACJGB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.denovo.248"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(20..91)
                     /locus_tag="IYI34_003160"
     tRNA            complement(20..91)
                     /locus_tag="IYI34_003160"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IYI34_003161"
     tRNA            complement(113..200)
                     /locus_tag="IYI34_003161"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IYI34_003162"
     tRNA            complement(225..298)
                     /locus_tag="IYI34_003162"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IYI34_003163"
     tRNA            complement(312..382)
                     /locus_tag="IYI34_003163"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IYI34_003164"
     tRNA            complement(384..459)
                     /locus_tag="IYI34_003164"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACJGB010000235        16749 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.066, whole genome shotgun sequence.
ACCESSION   DACJGB010000235 DACJGB010000000
VERSION     DACJGB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.066"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..414
                     /locus_tag="IYI34_003165"
     CDS             <1..414
                     /locus_tag="IYI34_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560945.1"
                     /translation="EEMIYFYQLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGM
                     MFHFVWNLQAYPFDFQLKNFFVWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILV
                     LEKIGIIDGYQHPNEKELLRTMTVFQQIVSEIRGN"
     gene            416..1675
                     /locus_tag="IYI34_003166"
     CDS             416..1675
                     /locus_tag="IYI34_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAR0560946.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1681..2262
                     /locus_tag="IYI34_003167"
     CDS             1681..2262
                     /locus_tag="IYI34_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAR0560947.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2344..3219)
                     /locus_tag="IYI34_003168"
     CDS             complement(2344..3219)
                     /locus_tag="IYI34_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAR0560948.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3367..3699
                     /locus_tag="IYI34_003169"
     CDS             3367..3699
                     /locus_tag="IYI34_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAR0560949.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3811..5019
                     /locus_tag="IYI34_003170"
     CDS             3811..5019
                     /locus_tag="IYI34_003170"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAR0560950.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            5173..5628
                     /locus_tag="IYI34_003171"
     CDS             5173..5628
                     /locus_tag="IYI34_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560951.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5990..6265
                     /locus_tag="IYI34_003172"
     CDS             5990..6265
                     /locus_tag="IYI34_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAR0560952.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6278..8356
                     /locus_tag="IYI34_003173"
     CDS             6278..8356
                     /locus_tag="IYI34_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAR0560953.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8516..10402
                     /locus_tag="IYI34_003174"
     CDS             8516..10402
                     /locus_tag="IYI34_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAR0560954.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            10414..11361
                     /locus_tag="IYI34_003175"
     CDS             10414..11361
                     /locus_tag="IYI34_003175"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAR0560955.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11381..11908
                     /locus_tag="IYI34_003176"
     CDS             11381..11908
                     /locus_tag="IYI34_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR0560956.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11971..12624)
                     /locus_tag="IYI34_003177"
     CDS             complement(11971..12624)
                     /locus_tag="IYI34_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAR0560957.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12756..13598
                     /locus_tag="IYI34_003178"
     CDS             12756..13598
                     /locus_tag="IYI34_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAR0560958.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13756..14652
                     /locus_tag="IYI34_003179"
     CDS             13756..14652
                     /locus_tag="IYI34_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAR0560959.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14655..15368
                     /locus_tag="IYI34_003180"
     CDS             14655..15368
                     /locus_tag="IYI34_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAR0560960.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15388..15906
                     /gene="msrA"
                     /locus_tag="IYI34_003181"
     CDS             15388..15906
                     /gene="msrA"
                     /locus_tag="IYI34_003181"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAR0560961.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15903..16130
                     /locus_tag="IYI34_003182"
     CDS             15903..16130
                     /locus_tag="IYI34_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAR0560962.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16282..>16749
                     /gene="lepB"
                     /locus_tag="IYI34_003183"
     CDS             16282..>16749
                     /gene="lepB"
                     /locus_tag="IYI34_003183"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAR0560963.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRS"
BASE COUNT         5813 a         2828 c         3468 g         4640 t
ORIGIN      
        1 gaagaaatga tctattttta tcaattagag gcagaaaacc tgtcaaatta tggtaaactg
       61 tttgaacaag ctttttatct gaccgaaaat ggccatctac tttctgctag tatgaaaaaa
      121 tttttaggaa tgatgtttca tttcgtgtgg aatctccaag catatccttt tgatttccaa
      181 ctaaaaaact tttttgtgtg gctggacttg cttggctatc caatccactg gctggaacag
      241 atttcaaaga tggagtattt ggaaaaagtt ctttttttgg cagaaaaaag caaaaaaata
      301 cttgttttag aaaaaatagg tataatagac ggataccaac atccaaacga aaaagaatta
      361 ttgagaacaa tgacggtatt tcaacaaatc gtcagtgaga taagagggaa ttgaaatgaa
      421 tacaaatagc gaaaaaatgt tgcaagcttt atcagatgaa gacttgtcac aagcacaaat
      481 ctttttagaa aaagcattaa aagaagaccc agcagatata ttagctgaac taggcgaaga
      541 attgctggct attggctttt tgatcgaagc aaaacagatt tttgaacaac tgatcaacca
      601 atacccagaa aatgacggac tgaacatccc gttagcagaa attgcaatcg aagataatga
      661 gatcgactta gcttttgaat acctggaaaa agtggataag caaagtgatt actatccgca
      721 aagcttattg gcgatagctg atttgtacca agttatcgga atccctgaag tgagtgaagc
      781 aaaattgaaa gaagcagctg ggttgcttcc tgatgaacca ttgatccagt ttgcgctagc
      841 tgaattgtat ttttcagttg atcgtttcaa agaggcagca gtggtttatg aaacattgtt
      901 ggcaagcggg ataaatgaaa tttcaggaat ctcgatgcag gaacgtttag gacaatcctt
      961 aagtatgcaa ggagaattcg aagcagctat cgtaccttta gaagaagcac tgaaagaaga
     1021 aaggacagat gatcgactgt tccagctagc ttttacgaat cttcaattga aagaaaatca
     1081 gcaggcaatc aactatctgg aagaacttcg agaagttaat ccgcattatc aatcgttata
     1141 tctatatcta ggacaagctc ttcaagaaga agaaatgatt gaagaagcac aaacagtgtt
     1201 agaagaagga atcaaagaaa atccttacca agtcgaactt tatcacctgg cctcggaaaa
     1261 tgctttccgt ctgcatgata gacagcgagc agaagaactt ttactgaaag ctttagaagt
     1321 aggagacaaa caagatgaaa cattacttac tttaagtaat ctttacttgg atgatgaaag
     1381 atatgaagat gtgatacaag tgatcaatca gatggaagaa actgctaatc cttatgcaga
     1441 atggaaccta gcccatgctt ataatgaatt agaagacttt accgttgcag ctgtacacta
     1501 tgaacaggct tatcatgaac tatcacatga accagatttc ttgaaagagt atgccttttt
     1561 cttaagagaa gaagggcagc tgaaaagagc gcaagaatta ttgacttatt atttaacact
     1621 tgaaccagga gatatggagg cactgtcatt attagatgac ctaacagaga ggtgagaaaa
     1681 atgctaattg atgtacggga aaagaagaat tttttaacgt ggatggtcaa tcatgtatct
     1741 tttagccgaa gagaagtttt ttggatcttg aattatcttg ccaatcatga agcgatttta
     1801 agcaatgtcc actttgttga aggggcgaat tttgcggatc gaggactgca gatcagtgat
     1861 ctatctgttg aaggggaacc catgaaattg tttttgcagg gaaaagaatt tacggacaca
     1921 gaccagattt ttcatgaaat ccgtttgaat tggaaaaagc ctttatatgt ggaatgtata
     1981 ttcgaaaatg cttggcagac aagagaatat ctatctatct tagaagacaa tccattttct
     2041 tcttgggatt cttcagtcag tgaagaagat actgaattaa ttgaacgata ttttcaagaa
     2101 aaagaagaag aggcaaaact aaaaatgctt tatgctcaaa tcgatcaagc actagaagat
     2161 ggagataggg atgctttttt gaaattatct gatgaagtta accgaaagat ccttttgcag
     2221 acacaaaaga aaagtaaccg ggtaagtgaa cataacaatt agttaaaaat agtatagtac
     2281 taaaaaagac aaaggtcagc tggatgtgga atctttcctc atagcctatc tttgtctttt
     2341 ttcttatagt tttttcagta atcttgtctg tggcttcaaa taagtaaagc cttgcccttc
     2401 cacttcatgc acattgatga ttgaaaggaa agctttttcg tcaatctcgt ggacgatcct
     2461 cttagcttca gttaattcac tagagctaac gaccatatag atgatttttt ttgaattacc
     2521 agagaatgcg ccttctccag aaaggaaagt cacacctctt tgcagaccgg tcatcagtag
     2581 tggtgcaatc tcttcgcttt tgttggagac gacaagtaga ccttttgcag aatatccgcc
     2641 atctaagatc gaatcaatca cacgactgaa aacatacgaa acaatcaacg tatacatcat
     2701 acgtttcaag tcgatatagg ttaatgaagc aagtaagacg atgatatcaa atgccaatag
     2761 ggaacgcccc atagtgatgc cgtaattttg ttctaaaatc cgagcgataa tatcactgcc
     2821 gcctgttgtt ccacccatgc ggtagacgat cccgcttcct acacctgcaa ctaaacccgc
     2881 aagtagagaa acgatcagta gatcatgctg taaattgatt tctagtggaa ccctttgcca
     2941 gatccataaa aagaatgaca aagaaagtgt acctagaaca gtataggcca aggaacgttt
     3001 accgaggatc tttcctccta gtaaaatcaa tgggatgttg attaataaag tcgtataagc
     3061 tggatcaata ccaaataaag ctcttaagat caaggtgatc cctgagactc ccccttctgc
     3121 taaatgatta gcgatgttca agtaaacaag accaaatgca taaatacttg ttcctataat
     3181 gataaaaaga acttctttta ctgtttttcg tttgaccata tgaattccag ctttcaatta
     3241 tgtattttca cagaatttcc atttagaaat tccttcctaa atgtagcatg gaaattcgat
     3301 tttctcaatt gctcttatct aggatttgcg ttaaagtttt ggtaagatag ttttggaggg
     3361 aaaaagatgg ataaaaagcg ttctttatac agtatgcagc aggaagtgga tgaatatatc
     3421 cagcaattca aggtggggta tttctctcca ttagctcaga tggctcgatt aactgaagaa
     3481 gtaggtgaat tggcaagaga agtcaaccac acttatggag aaaaaagcaa aaaggcttct
     3541 gaaccagtta actcggtagc agaagaactt ggtgatgttt tattcgttac gatgatcatg
     3601 gctaattcat tgaatatcga cttaactgaa gtttttgaaa aaaacatgga aaaattcaat
     3661 caaagagacc ataatcgctt tgagcgtaag gacgagtaaa caagatgaag tggtcctggc
     3721 cttgaagact ctgaaacgga cttgtcaaac agtcaccatg caactgagtc tttatctcag
     3781 gacaagaaac acgcaggaaa ggttggaaaa atgaaattaa aacaattacc tctggaatac
     3841 caaaaggcaa ttccagttct gaaaaaatta gagaatgcag gatatgaagc ttattttgta
     3901 ggtggaagtg ttcgtgatat cttgctgaac cagccaatcc atgatgtgga tatcgcaaca
     3961 agcgcatttc cagaagaaat caaacagctt tttccaaaaa cgatagatat tgggatcgaa
     4021 catggaactg tgttggtatt agaaaatgat gaacaatatg agatcaccac atttcggacg
     4081 gaatcaactt atcaggacta ccgtagacca gatcatgtcg aattcgttcg ttcgttggca
     4141 gaagatttga aaagaagaga ttttacgatc aatgcgtttg ctttgaaaga agatggagaa
     4201 atcatcgatt tgttcgatgg tctgcaagat ctagaagaac agatcttgcg agctgtaggc
     4261 aatcctcatg agcggttcca tgaagatgcc ttacgaatga tgcgcgggtt gcgttttgtc
     4321 agtcaactag ggttcaaatt agaggaagaa accttctacg ccatcaaaga aaaccacgag
     4381 ctattaggta aaatatctgt agaacgtata aatgtggaat tcatcaagat gctcttggga
     4441 aatgaacggg ccgcgggaat ccatgcattt attgaaacag agtgctacat ttgctgtccc
     4501 ggtttgaaag aagaaggcga agcactttta cgttttgctg aattaccatc cgtcccattg
     4561 aaagaagaga gccaagcatg ggcattattg atcagccaac tggatctaaa agaaaatgag
     4621 attcgttcct ttttgaaatc gtggaagtgt tctaaccaaa tgattaaaga cgttcaagca
     4681 cttgtccaag gactacgaca acgagaaact ggagctttgg aaccattcga attgtacttc
     4741 ttaggcaaaa aacatgcact gcaagtagaa gaattgatga tctattttga cgaaacacct
     4801 cagatggatg ccgtaggaga atcctatgat cgtttaccta tcaagtcatt aagcgaatta
     4861 gcagtagatg gtaaaatctt gctagaagaa actggaaaaa aacctggtaa gtgggttagt
     4921 gaagctttgc aattagctga aaaagccgtc gtagaacaaa gaataaaaaa tgataaaaaa
     4981 caggttttgg attttttatc gaaagaaaaa ttgatttaaa aaacactaga ctgtatctgc
     5041 tcagctttgt tggctactta aagtgggcag atacgtcatt agaagaaaaa acataagatg
     5101 tgaccactta cataaatttg tgctaaactg aggtagtaca agaataaatt acatcataaa
     5161 ggagtctttt taatgagtaa agaaatgaca gcgctaaaat tttattttag aaatggagaa
     5221 acctggacga tcaaccgccg tcatatcggc gatctttgga tcaaacaaat cacaacaagt
     5281 tttggacgaa tcaatggcag tgaatttgtt gaaatccatc cgtgtgctgg atttaaaatc
     5341 gaaatcttcc atgaagggga cgcagttgcg acacatgata tcaacctagg cggactggaa
     5401 atgggcatgt ttaatcgcgc attgaaatac gaagatatcg agcgaatgga aatcctttac
     5461 cgaaatggca cacctgatct tgtgtacttc ccatatctag ataaaggaac agaaggatta
     5521 gacaatcaat atcaatcaac aaaaatcagc gaaaaaacag gtaacttgta tatcgtgatc
     5581 aatccagatc aacgtgtaga agatgtatac ggggaatttt tcgaataaat cgatttttac
     5641 gcaaaaatga ggctgtgaaa gagatgtgtc ttattactac tgataggctg tctcttccac
     5701 accgtttatt cagaataagg gactgtgaca agatttttgt cgcagcccca tttttttgtt
     5761 gtgataaaag aacaagaaag gcaggaatgg gaaagtaaac atccttttgt cagaaacttt
     5821 tatctttctt aagttttact tgatatctga tactatttgg aatagaatga ggcgttgaaa
     5881 aatttatgga aatttctaac tagcaataaa cgatgaaaca atgaaatatt tttcatccaa
     5941 aaagaacttt gctataatga tggaagtttg tgtataggag atctggaaaa tgagagagag
     6001 ttttgaacaa caaaaaaaac tgttgcatga tcgctatggc gccctttcga tggatgaccg
     6061 ccgtcaaata ctttgtaagt tgcgtaaaag aaatatattg atgtatcgtc agttggaacg
     6121 tctgaaacat gatctgcttc gtttggagtc aaaacgtgta cagtgtgaat tagaggggaa
     6181 ccaaacacag gttgaagtag tcgaaaccaa aattttaaag aaaaaagaac aatttttaaa
     6241 aatgcttaca caaaataaaa aataaagggg atggacgatg gaatttgctg agttagttat
     6301 tgtatttgca gtaacgatca ccttatcgaa catagctagc cgcattttgc caatgattcc
     6361 agctccactg atccaaattt tccttggagt ggtattaggt ctgacagagt ggggacaatc
     6421 aatcgatttt gaacctgaat tgttcttagt tatgattatt gcaccattgt tatttagaga
     6481 aggagaaaaa gcagatattt cttcgattct caaaaatttc gggacaattt tgttcttggc
     6541 atttggagga gtcattttga cccttgtcgg ggtaggtgcg acgttatcat tcctgttgcc
     6601 aagtgtccca cttgctgcct gctttgcttt tggagcagcg ttaggtccga cagatgcggt
     6661 agcagtcagt tctctttctg gacgagtgaa tatccctaaa aaggcgatgc atattttgga
     6721 aggagaaggt cttttgaatg atgcatctgg tgttactgcc ttccagtttg ctcttggtgc
     6781 gctgatcacc ggatcttttt cagcagtaaa cgcggggatg agtctagtag tttctagtat
     6841 tgggggagct ttgatcggct ttttgcttgt ctggttcaaa caaaaaataa ttcatttgat
     6901 cgaaaaagca tcagcacaag atgtcacagg gtatctgctg atcgaacttt tgcttccttt
     6961 tctagcctat gtattggcag aggcatttga tgtatctgga atcattgctg cagtggcagc
     7021 aggtattctc caagcttctg gttttaggaa aatctctgtg tttgatgcag aattgtcgag
     7081 cttgtcccac agtacatgga caactattgc ttttacactg aatgcattag tatttatatt
     7141 tttaggaatc gaattgacgc aagtgttctc gccagtatgg ggagacggtt tatatccaaa
     7201 cggtctttta ttagcgatta tcgtattaat ctctgtcatg ctttttgtga tcagattcat
     7261 ttctatcagt cttttttatg tattcaaaga tggcagtaaa aagttcaaaa aacagttgaa
     7321 cgagatcttg atcttaacat ttggcggtgt aaagggaaca gtcagtttgg ctacgatttt
     7381 tattttgcca ttttcaatca ataatatgat gttttaccag cggtctttgc ttttgtttct
     7441 gacggcgggt gtcatcttag tgactttggt catagggatc attgtcttgc ctatgctgac
     7501 ggaaacagag gaagcgaaaa gcacggattt gaatgctttg atgattttag aagaggtcgt
     7561 tgaagttttg cgaaaagaaa taaaggaaat tgatcgtaat acaaaagaat ttttagccac
     7621 tgaagcagtg atagaaaatt atcaagaacg gatacgtgat ctgtacttgg aagatttaac
     7681 cgatgatgaa aagcaagaag tacaagaaat acaagcactg attttgtcga tcgaacggga
     7741 tggattggat gaaagttatc gttcgggcaa gctttcttct aatggttatc gtttttactc
     7801 ccgttttctt tctcgattcg aacattctat cacaagccag attttgtctt ttatcggctt
     7861 ttggttcatt gttgtccgta ggttaatgcg aatcgttctc catccgaaaa tgttttggca
     7921 aagaagatat gctgatcgtc agacatttat tagtgatgaa gatatccaag aaatcagaga
     7981 gacatatcaa aaaaatactc agttgattat tgaaagttta gataacttga ctgatattta
     8041 tgatgaaacg atggttcaat tttttatcca gcaacgcaaa cttgaaggaa taaaaatgaa
     8101 ctcggggaat ttgatttctt cgtggatgat ccagcaagat agtctgttta cgaaaaaaat
     8161 gctaagaggt tattatcttg aaagaaaaat cattgatgaa tacgaagttg ctgaaaaaat
     8221 cacgacgttt tctgcgaaca gctatcgcag gaatatcaat ttgttagaat cttatacgat
     8281 gaacaaacca tctgataact tttctttccg atttgcgttt cgagcaaagt caaaaaaaac
     8341 gagtccaata aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa aacatatcag
     8401 aacaggatcg aatcggtcct gttctttttt tgagaacaca aaaagaacaa ataccaaaaa
     8461 aattttcctg aagttcacta tttttatgat acaatatcca agataaggag ggaacatgtt
     8521 gaaagaatta aaagtaactg acctcaaaaa aacttatggt gaaaaagatt tgtttgacca
     8581 gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga acgggacggg
     8641 taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg atcggcaaac
     8701 agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg aattttccga
     8761 tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc aaactgttcg
     8821 agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa
     8881 acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta cagataccaa
     8941 cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa tcagtacttt
     9001 atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg aacccgattt
     9061 actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt ggttggaaaa
     9121 ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt attttctaga
     9181 tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt ataaaggaaa
     9241 ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga
     9301 aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg tacaggcacg
     9361 tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga acttgtacca
     9421 agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag ggaaaaaagt
     9481 actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag aacatttaga
     9541 tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg cgggaaaatc
     9601 gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa tgtctatcgg
     9661 tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac cagataaacg
     9721 gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg gcagtcaaat
     9781 cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg ggacactgat
     9841 aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc tgattcagca
     9901 acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa cattgacgat
     9961 tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc atgaccgtta
    10021 tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa aacaagaact
    10081 ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc agcctgctga
    10141 aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat tatcttacat
    10201 ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc
    10261 attgctccaa gaagagatga atcatcaagg agataatttc actcgattac aagaattgca
    10321 aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt
    10381 gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga
    10441 agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc
    10501 ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa
    10561 cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat
    10621 atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac
    10681 atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca
    10741 gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta
    10801 accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg
    10861 cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca
    10921 atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt
    10981 cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg
    11041 ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt
    11101 gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa
    11161 tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa
    11221 caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca
    11281 gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc
    11341 aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc
    11401 acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga
    11461 tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt
    11521 cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc
    11581 ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga
    11641 aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct
    11701 gccattttgt acgatattat accgtacagt catccatcat gtattcgatg gagatgctta
    11761 ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga
    11821 tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa
    11881 aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga
    11941 gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat
    12001 aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt
    12061 caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac
    12121 accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga gccaccccat
    12181 aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca acgtcaacgc
    12241 tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag tccaacctag
    12301 ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca aaagaaaaat
    12361 cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa
    12421 caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat aagagacaat
    12481 atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc ccgcgatact
    12541 caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata tttcatacgt
    12601 tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa
    12661 atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt taacatgaat
    12721 aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa atcgtaacag
    12781 attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc
    12841 tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac ggcgaaagat
    12901 ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca ccaattggag
    12961 aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt tcgatccata
    13021 tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat ctaacaaaaa
    13081 cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt caggtaattc
    13141 gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa
    13201 aagtacaaga caatacaaaa ttatacatcg gaatatccac actagataac ttggtaaaag
    13261 gtggaagaat cagccgaaca actggtttgc tgtctagtgt tttgaacata cgcgtggtga
    13321 tgaatttcgc ccattctgaa ttaatccctg taactaaggg gcgaggaaag aaaacgttcg
    13381 ataaatggtt taataatttg aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg
    13441 gtatttcaca tgcggatgca aaagaattgg cgcaagaatt caaagagggt ttgcaagaat
    13501 tattcccgga catgcatatc cctgtactgc atacgaatcc ggttatcgct acgcatactg
    13561 gtaaagacgc gtttgcaatc atgtattatg tagattagtc gaaaagagga taagccctca
    13621 gcctgaaagg cttgcagctt attctctttt tctttataaa attaggaaaa taattgtttt
    13681 cttttttcat aaagactacc tttttgataa aatttttgat aacattagaa ccaatcgaat
    13741 acaagaggtg aaaaaatgaa attaacgaaa cgacatttga tcctcgtttt gttcccatta
    13801 ttaattgcga tcatcatcta tgcaggttta gctgcttcta taccgaaagc tggcccacta
    13861 atgaaaccag taactactgt gagagaagat ccaaataaaa aagaaaaact tcactacata
    13921 gctatcggag attcgctgac tgaaggagta ggagatcaaa ccaagcaagg aggattcgtt
    13981 ccccttgtcg caaatgatct aaaagaccgt tataatttga ccgcggttga aacagaaaac
    14041 tatggtgtaa atggtgaacg aagtgaccag atcttaaagc gagtaaaaac aaaagaagaa
    14101 attcgaaaca atatcaaaac agctgatttt atcacactga ctgttggcgg taatgatttg
    14161 atgaaagtta tccaaaataa tttattcggg ttaacggtca attcctttaa aaagccactg
    14221 aaaaactatc aggaaaatgt cacagaacta ttggaagaat ttcgatccct caataaaacc
    14281 gcacctattt acattttagg catctacaat ccattttatt tgaactttcc tgagatcact
    14341 gacatgcaga caattgttaa caattggaac aaaggaacag aaacggtgac aaaaaaaatg
    14401 ccgaactgct actttatccc aatcaatgat ttgctttatc aaggattgaa tcaggaagta
    14461 ggtgtcacag aagaaggaac agatcaaaca actagcacga cagaaaatct aaatggcatc
    14521 aaaaataatg tgctctatga agaagatcat ttccatccaa ataatttagg ttatcaattg
    14581 atggcaaatg cagtgagaga tcagctgatc gagacacaaa accaatggtt agagaaggag
    14641 gatgatcaat aaacatgaaa acaagatctg acgtatcttc aaacaaaaca aagaaaacaa
    14701 attactggaa attcgccttc cttatccttc ttggggtagt acttggttcg accgtatttt
    14761 taggcagccg tatttttgct aatcgggaac cagaactccc agaaatacca gctcttacgg
    14821 aaagacaagg agatcctgtc ttaacgatca attcaaataa agaaaaagtc aatcaaatca
    14881 tcagcttttt ccttagtgag tatcaaaaag atagtgatat tgaatataaa ttttatttgg
    14941 aaaatgaagc actactcaat ggtactttcg aagtactagg ttttcctatc gatttctatt
    15001 tatattttga tccgtatgtg atggaaaacg gaaatgtcca actaaaagct agaagtcttt
    15061 ctatcggaac attgagtttg ccaatcaaag atgtgatgaa tatgatcaag agaaactata
    15121 aattaccaga ttggatcgaa gtcaatacag atgatctgac tatcatgctt aggttggatc
    15181 aattccgaat gcaaaacggg atgtatatta aagcagataa aatcgactta gtaaatgatg
    15241 acattcggtt tagtctatat ttgccaaaag atacctctga tgcttcaaac aactcaaata
    15301 ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa agaaaataaa aaatcatcgg
    15361 atgagtagta ttagaggggg aatcataatg gaacgagcaa tatttgcagg aggctgcttc
    15421 tggtgtatga tccagccttt cgacacgctt cctggtatcc atacagtgat gtcaggatat
    15481 actggagggc atgtaccaga tccgacttat gaacaagtaa aaagccaaac gactggacat
    15541 acagaagcag tggagattat ctatgatcca acattgattt catatgaaca attagtagaa
    15601 ctttactggc agcaaactga tccaactgac gctttcggac aattcgagga tcgcggtgac
    15661 aattatcgcc cggtgatctt ttatttgaca gaagaacagc ggatcattgc tgaaaaaagt
    15721 aagaaacagt tagctgaaag tggacgtttc gaacagccaa tcgttacaaa aatcgaacca
    15781 gcccaaccat tttatctagc agaagaagac catcaagagt attataaaaa gcatcctgaa
    15841 aattttaaaa gaaaccatgc acgccgggct gcctttatcg aagaaaattg gaggaacgaa
    15901 gggtgaatcg aagctttttt cattatttaa tgacactgcg aggcggtggc acacatgatg
    15961 ctttatctgt tttcgctaca gaagccggta aagacatcca atttcctaag cacagctcta
    16021 gctacgaaga aatttctaat tatctggaac tgaatgtcga ctatttgccg tccatggata
    16081 tctttgacga agcatgggaa aaatatttag aaaataatca tgacatataa gagatagttt
    16141 tgtactttat gatcaaaaaa gttttttgtc caatagccta attgaaagaa ttatttttat
    16201 ttcagctatc tagtcgcatt tttttgatat ttttcttata ataaagaaag aatgaaaaaa
    16261 agagggatgg attgatagag attgacaaaa aaacaacgct atattaatcg cttctggctt
    16321 gtattcaaat acttacttgt ttctgtattc cttgcgttca tgttgcgggg atttttattc
    16381 atcccggtac cagtggaagg caattcaatg gaaaacgtgt tgaagcaagg tgatatggtt
    16441 gtgatggaaa aattttcgga gatcagacga tttgatattg ttgtttttca attagcagat
    16501 ggcacgattt atatcaaacg agtaatcggt cttccaggag aaaatgtcag ctaccaaaat
    16561 gatcaattga agatcaatgg gaaagtagtt aaggaacctt atctaactaa aaacctgaaa
    16621 tcagatcatg caaatgcatc ctatacgacg gactttacac tacaagagct aacgggacaa
    16681 tccaagcttc cggaagataa ttattttgta cttggagata atcgaagagt ttcaaaagat
    16741 agtcgttcg
//
LOCUS       DACJGB010000236        15884 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.071, whole genome shotgun sequence.
ACCESSION   DACJGB010000236 DACJGB010000000
VERSION     DACJGB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.071"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(134..577)
                     /locus_tag="IYI34_003184"
     CDS             complement(134..577)
                     /locus_tag="IYI34_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAR0560964.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IYI34_003185"
     CDS             complement(795..2135)
                     /locus_tag="IYI34_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0560965.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IYI34_003186"
     CDS             2404..3300
                     /locus_tag="IYI34_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAR0560966.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IYI34_003187"
     CDS             complement(3450..4391)
                     /locus_tag="IYI34_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAR0560967.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IYI34_003188"
     CDS             4517..5638
                     /locus_tag="IYI34_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAR0560968.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IYI34_003189"
     CDS             5896..7398
                     /locus_tag="IYI34_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAR0560969.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IYI34_003190"
     CDS             7465..7992
                     /locus_tag="IYI34_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAR0560970.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IYI34_003191"
     CDS             8203..9264
                     /locus_tag="IYI34_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAR0560971.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IYI34_003192"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IYI34_003192"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAR0560972.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IYI34_003193"
     CDS             10177..12159
                     /locus_tag="IYI34_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAR0560973.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IYI34_003194"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IYI34_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAR0560974.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IYI34_003195"
     CDS             12881..14305
                     /locus_tag="IYI34_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAR0560975.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IYI34_003196"
     CDS             complement(14486..15259)
                     /locus_tag="IYI34_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAR0560976.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..>15884)
                     /locus_tag="IYI34_003197"
     CDS             complement(15256..>15884)
                     /locus_tag="IYI34_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAR0560977.1"
                     /translation="YDHLKLFSETNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKL
                     GIALALLNHPSIVILDEPMNGLDPQATRDVRKIITDLATQGTSFLISSHLLSELEKMV
                     DELILINQGKIIKQCTMAELNQTAQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLL
                     KENEELLGTVIQLLSAQNIHVTDVQHLTNDLESSLLEILDSVKGEEEK"
BASE COUNT         5111 a         2881 c         3308 g         4584 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagc
//
LOCUS       DACJGB010000237         9497 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.097, whole genome shotgun sequence.
ACCESSION   DACJGB010000237 DACJGB010000000
VERSION     DACJGB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.097"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(565..792)
                     /locus_tag="IYI34_003198"
     CDS             complement(565..792)
                     /locus_tag="IYI34_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAR0560978.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(801..1274)
                     /locus_tag="IYI34_003199"
     CDS             complement(801..1274)
                     /locus_tag="IYI34_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAR0560979.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2504..3070
                     /gene="amaP"
                     /locus_tag="IYI34_003200"
     CDS             2504..3070
                     /gene="amaP"
                     /locus_tag="IYI34_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAR0560980.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3083..3271
                     /locus_tag="IYI34_003201"
     CDS             3083..3271
                     /locus_tag="IYI34_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAR0560981.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3284..3841
                     /locus_tag="IYI34_003202"
     CDS             3284..3841
                     /locus_tag="IYI34_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAR0560982.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3873..4115
                     /locus_tag="IYI34_003203"
     CDS             3873..4115
                     /locus_tag="IYI34_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAR0560983.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4456..5670
                     /locus_tag="IYI34_003204"
     CDS             4456..5670
                     /locus_tag="IYI34_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAR0560984.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5701..5919
                     /locus_tag="IYI34_003205"
                     /pseudo
     CDS             5701..5919
                     /locus_tag="IYI34_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6338..6811
                     /gene="dfrF"
                     /locus_tag="IYI34_003206"
     CDS             6338..6811
                     /gene="dfrF"
                     /locus_tag="IYI34_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HAR0560985.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            6938..7594
                     /locus_tag="IYI34_003207"
     CDS             6938..7594
                     /locus_tag="IYI34_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAR0560986.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            7776..8306
                     /locus_tag="IYI34_003208"
     CDS             7776..8306
                     /locus_tag="IYI34_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAR0560987.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            8852..9094
                     /locus_tag="IYI34_003209"
     CDS             8852..9094
                     /locus_tag="IYI34_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HAR0560988.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            9091..>9497
                     /locus_tag="IYI34_003210"
     CDS             9091..>9497
                     /locus_tag="IYI34_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAR0560989.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIGSLSNDVGKEKS"
BASE COUNT         3343 a         1380 c         1866 g         2908 t
ORIGIN      
        1 ctcgagttac gagtttggaa cagcttttgt ctacagtctg tcattattat caaaataatg
       61 ttaaaaataa aaaagaattg tccatcactg ttattcaaat cattttatca aaactacata
      121 gtaaatgtta aaaagcaaca tgacataata tatagaaggc tttacagtat atatgggagc
      181 ttactaaaaa agaatggagt aagagttaac acaatgggcc attagctgaa gttttaagca
      241 cttcaaggta tgaaacagaa tggcctgtaa tactacatga ttatttagac tgctcaaact
      301 gctatcagtc tggtggaatg aataaagtat tatactaaaa gcagatccaa ctacaacgta
      361 acaggaaacc agttgtatca ggaaaagtga tagtacatgg agaagaaaag gccccctttg
      421 tatgagtcag tacatatctt atttatagat aaaacggtat tcaatcgaaa taaaaaatct
      481 gaataataga cgtaaataac ttctaaaagt ctcacacatc tatcattcag attatttttt
      541 atttactgtt tgttctactt tattttattc ttctggtata gtattcgagt catttttata
      601 ggtcaacaaa tcacctggtt gacactccaa aacttggcag attttttcga gagtggaaaa
      661 acggatggct tttgcttttc cattttttag aatagagatg tttgccatcg taatgcccac
      721 tttctcactc aattcagtca cactcatttt tttttgagct aatactacat ctaaattaac
      781 gataattgcc atgtcgcacc tcacactgtc aaatcatgat ctgtcttaag taaaatcgca
      841 tgttcaagca gtttttccag cacgcttgtt aatgtgtata ctgcaaaagg aatgaatacg
      901 acagcgattc ctaccaacat caaacctgga gcatcgtcta aatccgctac tttcataaat
      961 ttaggaagta agccaaaaaa ggcgaaacca ataaacaaaa tggttcgttc gatttttttt
     1021 agtatgttta agctttgagt ggaaaaagct tggttatctt cgattttttt caatagtttc
     1081 aataagaaat aagcaataac gatccctagg agaagggcaa agtaaatgac agaaacaaat
     1141 aatgtggtct gccaatcaaa attgaatcct ttttcgcttg tagcgatctg agtataaaaa
     1201 agtacgccga acaaaataaa tacggcactt atacctaaaa cgacaaattt taatagcaat
     1261 gttttaaatt tcatttttat ccctctttta tctttgatag ctaaaatata gcagaataat
     1321 tattgtaaaa caactatttt ttatcgaaaa acaataaaaa tatatcgttt ataaaaaatg
     1381 atagagtatg aaggtgtgga gtgcatttaa cagctttttt tgattgtaag aaacatttaa
     1441 aaccagttat tttagattct atgtgtgtct cttctataat gctagtttag atgcctggga
     1501 ttatattgga caatacagtt ggaggatttt ttattatcta gcgaagagaa gtgtaagtta
     1561 acagaactgt atgagaaaaa agacaactac gtagtagcta accgattcat taattttttc
     1621 aagttcatgt ttatttacgt atcagaattt ttcttattca atggtttggt gtaatcacca
     1681 cttatttgtc ctttattgct tatacaatgt tatacaaaag gaaaatcaag cattgacgca
     1741 tggggtggta ctatctagct gaaatagctt aataagcgca agtagatgca ttggattagt
     1801 ttataaatca aaagtgttag aggtattaag aagaagtaga aaaataaaat ggattgccga
     1861 ataaaaagta ccataaatcc taaaacatgt gccaacagat agatggcaca tgtttatttt
     1921 tttcgataat tttgtaacaa aaatacgaag atgaaaaaga atagagattc ccataatgta
     1981 gtagtccaac tgaattcttt actgatacca aataccatat ttcctagatt tgatacaatc
     2041 aaatagccaa tcgttatgat caatgaatag tacaatcttt tcatcatagg acatacctcg
     2101 tttttattta taagaatagt ataaaccaaa gaagtgaata agaaaaatga taatataaaa
     2161 ataaagtaag aacgcattaa agtaactaaa gttatttatt gtatttttta attctccgaa
     2221 aaaatttaat aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt
     2281 aatcttgaat aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa
     2341 gcaattgtta tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg
     2401 gtagcgcttt atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt
     2461 agagttattt acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa
     2521 aataatcggt gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat
     2581 caatgcgccg tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa
     2641 ttactacatg caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat
     2701 catcttgcta gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga
     2761 agatggcaaa ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca
     2821 cgaagaagag ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca
     2881 tatccatgtc aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt
     2941 gatgcaagat atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt
     3001 ggcagtcaca tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg
     3061 agtggaatag gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat
     3121 ctttgggggt cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac
     3181 attgttgatc ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc
     3241 aggaatagtt gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat
     3301 gaatacaaca aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg
     3361 tgaattgact tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt
     3421 agatggtcta ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa
     3481 cacagataac gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga
     3541 tctagatgtg atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga
     3601 attgatcagt actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa
     3661 tgttgcagac atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa
     3721 agtaacagaa gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa
     3781 atctgctgta ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata
     3841 agaagtaaga agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg
     3901 gcggtatcat tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca
     3961 tcgcaaacat cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt
     4021 ggggaccaac aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag
     4081 tattagtcgt ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg
     4141 aaatgaagaa caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
     4201 tatatgaagt attctttatt ccttatgaaa aaatatcttt aaaaataaaa aaagacgaaa
     4261 ataaacggtt ttctataaaa agaagaccgt ttattttttg tctttatttg tgtgaaaaac
     4321 gaaaaagagc acaacacatc aagcttttat taattgtctg tcttttataa atgcaagtat
     4381 taattcaaaa aaacagaaga aaaaaacaac tacaccttct tttatactcg taatcgtaga
     4441 aaggaggcga aaaaaatgaa tacattacct atcaatacag gagatacagc attcatgatt
     4501 ttatgtacag caatggtttg cctgatgact cctggtctag ctttttttta cggcggatta
     4561 gcacggaaga ataatatact aacgatcatg tctcaatctt ttctctccgt tgggataaca
     4621 acgattatgt gggtatttgg tggattcgga ttggcctttg gacgggatat cggcggtgtt
     4681 attggaaacc cggcagattt ctttttgatg agacatgtta caagcttccc caacatgttt
     4741 catggtgcaa cgattccatt tttaatgttc tttctttacc aactgatgtt tatcgtgatt
     4801 accgttcctt tgatgacagg agcttttgca gggagattga atttaaaagg ctatattata
     4861 cttgttattt tttggaatct gctgatttat ttccctgttt gccactggat atggggtggc
     4921 ggatttttgg atcagatggg atttagagat ttcgctggtg gagcagtcat ccacacgaca
     4981 gctggattcg gctctttagc atgtatcctc acacttggtc aaagaaagat tcctttagga
     5041 gaaagaagtc agcatagcag tcttatggca gctgctatcg gtacaggact gctttggttt
     5101 ggctggttcg gttttaattc cggtggtgca cttcgagcag atgaacaagc agtcaatgca
     5161 tttggttcaa cattcatcgc tcttgcattt gcgatgatta cttggctgat cattgcaaaa
     5221 gtaaaaggaa atggctttga ttttgtcgac gtactaacag gatccgttgc tggattggca
     5281 gccattacgc cttgtgccgg ttatgtagaa gcaaaaagtg ccatgctgat tgggatcatt
     5341 gcgggtatcg tctgtcatgc tgctgttgat tttcgtaaaa agaaacaatg ggatgatgca
     5401 cttgatgtgt ggggtgttca tggcatgggc ggattcacag gtacgattct gatagggatc
     5461 tttgcatcta acagccattt actaacgaat ccaagcagct ggtacttctt agggattcaa
     5521 gtgattggtg tcatcattac tgccgtgtat gcctatgtgc tgacaacagt gattttaaaa
     5581 tcggcgatgc actttacaac gatcactact acaaaagaag aacaagaaga aggattggac
     5641 tttttcttct ttggcaacaa actcaattga aacaaattca aatagaaaag aggaagattc
     5701 atgcaacaaa gcaaaacgaa aattcctgca aaaaaattaa cgttctttgg ctttttggct
     5761 atgaccatct caatggtcgt ttcattatat gaatatccca cattcgcgac ctcaggcttc
     5821 agcttagtat ttttcttgtt attaggcggt ttgctatggt ttatccctgt ggcattgtgc
     5881 gcagcagaga tggcaacggt gaaaggatgg gaaaaaggtg tttcccaaac aaaaagatat
     5941 taagaaacag cccatgttta cctgcttgca aacaaaagaa tattgctact agactaagag
     6001 atactacata agtgtgttgg tatctctttt ttgttgccgt gaaacagcag actatttcat
     6061 ttctgcttga ttaccttaaa acttattagt ctatggtttg tcaagggctt gttgcgtgag
     6121 aaatgctgaa tatcaccctt tacaaaacat aggctgataa gtaacttttc aaaagcagaa
     6181 aagaaatggc aatgcttata acaatatgat ataatgcaga tgaagacaaa aaaggaattt
     6241 gtcgagctgg ttagttcgag ataattttga gtttgttaag atgtggtgca cttgatggaa
     6301 tcacaggtac gacatcatat tatggaggat gaaatagatg ataggtttga ttgttgcgag
     6361 gtcaaagaat aatgttatag gcaagaatgg taatatacca tggaaaataa agggagaaca
     6421 aaagcaattt agagagttaa caacgggtaa tgtggttatt atggggcgaa agtcttatga
     6481 agaaatcggt catccgttgc ctaatagaat gaatattgtt gtttccacca caacagagta
     6541 tcaaggagat aatttagttt cagttaaatc attagaagat gcattattat tggctaaagg
     6601 acgagatgta tacatatctg gtggatatgg actatttaag gaagctttgc aaatagtaga
     6661 taaaatgtat atcacagaag tagatttaaa tattgaagat ggagatacat tctttccaga
     6721 atttgatatc aatgattttg aagttttgat aggggaaaca cttggtgagg aagtgaaata
     6781 tacgagaaca ttttatgtaa ggaaaaaatg aattgagtaa attttggatt tagggtgttt
     6841 tcataaatat attgcttttt atgcatatat aaattgttgt aagacaaatt ccagtttgta
     6901 tggggcgata aaatcagaat ttgagagagg attgaatatg aataaggaat ggtctgaact
     6961 taataaaaca atgcaggcac aaataaaaaa gaaagatact tataagaggg gtattgatac
     7021 tttacttact ttacgaagtc agttaataca aaccttagta tctttcaaag aagaattatg
     7081 cagagaagat tttaactcaa tccccttcat aaacgctgat ggttatcata gtaagacgat
     7141 tgcttattct atttggcata ttttccgcat tgaagatata gttgtgcata cagtgataaa
     7201 tgaagatgaa caagtatttt ttgcaggcaa ttatcaagag cgtatcaatt cacctatcat
     7261 tacaacagga aatgaactca tgaaacagca gattgcagac ttttcaaaac agcttaatct
     7321 ggaagaatta tatttatata ttttcgaagt atgggaaagc actgagaaaa tgcttgaacg
     7381 gttatcttat gatgaattga aaagaaaaat accgaaagaa agaaaaggat acttagaatc
     7441 gttgaatgta gtaaacgaca atgaaaaagc aatttggctg attgattatt ggtgtaataa
     7501 agatatttgt ggactaattc aaatgccgtt ttcaagacat tggattatgc acacagaagc
     7561 ctgtttgcgt ataaagaaca agatacattc ataggcaaaa atttcagttt gtagaactaa
     7621 ggaaattgga atttaaggag gaataaagta tggttagtga ttttataaga gcagcatttc
     7681 catggatttt aatgggttta tttgtagcaa tcagttgttc ccttatgagt aaaaaagaga
     7741 aataaattcc tgtttgtatt tataaaagag aaaaaatgag aaaagtagtt ttatttattg
     7801 ccatgagcct tgacggatat attgcggacg gtaatggtgg agtagcttgg ctaaatggtc
     7861 atggaaatga caatgaaaac atcgacactt atacagaatt taccaaagat atagatactg
     7921 tcttaatggg gtggaatact tctcatcagg ttgttactga actttctccg caagaatggg
     7981 tatataataa atttacaact tatgtattaa cacataaaga gtgcaattct tcccaacaaa
     8041 ttcattttac aagtgaaaat cctgttttat tattggaacg gctaaaacaa gaagcaggaa
     8101 aggatatttg gatttgtggc ggagcaaatc ttgttcagca gttggtaagc aaaaatatga
     8161 ttgataaata ttacatttct gttattccta ctctgttagg aaatggggtt cgtcttttgg
     8221 gcaacataga cagggaaata aagttaaggc tttgtaaaac acagacttat aacggaataa
     8281 ccgatttgat ttatatgcgt agataactag agagaaacag aaaagctgtt aaaccgacgg
     8341 agcgatccga ccacgttggc gggaaaatcc ttaagatttt tccgccatgc ttgcaagggg
     8401 gcggttcgct tcggcggttc ggcgagcttg cgagccgttc aaagcccccg ttatctaaca
     8461 aggggggcac aaaaaacaag aaacactata cactaacaag cggaaaacgc tgtatttata
     8521 aggcaaaacc ccatttttac agtagtgata taaattgtac ctatatcact atgaaaattg
     8581 aatagtaaat atgagaaaga cagttgattt caaaaaataa gaaattgatt gtcgttttct
     8641 tttgcagaaa tttaagtgca ggcaattatg gtagttgtcg taattgctgt cagctcagtg
     8701 tttatcactg atggtgtgaa agatttacgg ttggagatgt tcaataagat tattggtgct
     8761 taattatttt tattgtagtg ctgatatgag aatgttataa ttggggtata aaacatacaa
     8821 ataaatttgc tgaaaaatga ggtatcaaaa tatggaaaat gaattactgg aaaacttagg
     8881 aagacttcac acaactgaac ttggcgttat tagaattaaa aagaacttat ctttgaatgt
     8941 agaaaatgta attgaatggt gcaaagaaaa aattagcctt tctaatgcgg aaatcacaag
     9001 aaaaggaaaa aactggtata taacgattga taattgtatt ataacggtaa atgcgtacag
     9061 ttataccgtt atcactgctc acaaggtaaa atgataaatt tttctataaa ggaattaaac
     9121 gcatttgcag ttattgggca ggaagtagaa ctgactaatt atcaaaaaaa gaatattcag
     9181 attagtacac agttttggag aaagtttaac agtagtttga aaaaagcgta tctttcacaa
     9241 tcaggaaatt gggtaaaata tgcttttatg gaaagaagaa atggaaaact ttattatttc
     9301 tgttctattc cccaaagaac cattacccca gagggctttc tgtataaaga aataccgtct
     9361 tataaatatt tggttgtgga gcatattggt gctatggata aaatatatga aacttatggc
     9421 aagatttatg aagaaattat tcctagtaca tcgtatattg gctctttgtc aaatgatgtt
     9481 ggtaaagaga aatctta
//
LOCUS       DACJGB010000238         8782 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.099, whole genome shotgun sequence.
ACCESSION   DACJGB010000238 DACJGB010000000
VERSION     DACJGB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.099"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..511
                     /locus_tag="IYI34_003211"
     CDS             <1..511
                     /locus_tag="IYI34_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAR0560990.1"
                     /translation="FEVIHQIQAENEEPLAALNTGYHEPADIRKRLENIISDKVDDTV
                     TVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLEDSVLIGPRVNLITVNHLTDPK
                     ERRGLLVKPIHIKKNAWIGAGATILPGVTIGENAIVAANATVTKDVPDNTIVAGIPAK
                     IVKPVERII"
     gene            578..1315
                     /locus_tag="IYI34_003212"
     CDS             578..1315
                     /locus_tag="IYI34_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAR0560991.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            1546..2814
                     /locus_tag="IYI34_003213"
     CDS             1546..2814
                     /locus_tag="IYI34_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAR0560992.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            3301..3765
                     /locus_tag="IYI34_003214"
     CDS             3301..3765
                     /locus_tag="IYI34_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAR0560993.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            3749..4378
                     /locus_tag="IYI34_003215"
     CDS             3749..4378
                     /locus_tag="IYI34_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAR0560994.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVTRDHGYETNPI
                     GGVEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            4732..6210
                     /gene="msr"
                     /locus_tag="IYI34_003216"
     CDS             4732..6210
                     /gene="msr"
                     /locus_tag="IYI34_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAR0560995.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            6257..6703
                     /locus_tag="IYI34_003217"
     CDS             6257..6703
                     /locus_tag="IYI34_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAR0560996.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6902..7417)
                     /locus_tag="IYI34_003218"
     CDS             complement(6902..7417)
                     /locus_tag="IYI34_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAR0560997.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            7534..8736
                     /locus_tag="IYI34_003219"
     CDS             7534..8736
                     /locus_tag="IYI34_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAR0560998.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         2811 a         1535 c         1836 g         2600 t
ORIGIN      
        1 ctttgaagtg atccatcaga tccaagctga aaacgaagag cctttagcag cgctgaatac
       61 tgggtaccat gaaccagcag atatccgcaa acggctagaa aatatcatta gtgacaaagt
      121 agatgatacg gtgactgttc tgctgccttt ttatacagat ttcggcaaac atattagtat
      181 agggaaaaat gtctttatca atcgtcaagt gatgttcgtt gacttaggtg gcatctgttt
      241 agaagattca gtattgattg gtcctcgtgt caacctcatc acggtgaacc atctaaccga
      301 tccaaaagaa cgaagaggct tgttagtaaa gccaatccac atcaagaaaa atgcttggat
      361 cggagcagga gcaactattc tgccaggcgt aacgattggc gagaatgcca ttgttgcagc
      421 gaatgcaact gttacgaaag atgtcccaga caataccatc gttgcaggaa tccctgccaa
      481 aatcgtcaaa cctgtcgaac ggattatttg aacagtaaga tgaaataaga gatagtgagt
      541 ataagcaaga gatttttcaa aacaggagga gaatagaatg aatgatttat cagaaaaagt
      601 gattgtcatc atgggcgctt caagcggaat cggtgaagca acaacgaagc tattagcaga
      661 aaaaggggct aaactagtta tagctgcacg ccgagaagat cgcctaaaag caataaaaga
      721 atctttacca gaagctgagc tatatattca aacagcagat gtaagagatt ttgcacaagt
      781 ccaagcagtt atcgatttag cgatggaaaa gttcggtcga atcgatgtat tgtacaataa
      841 tgcaggaatc atgccaaccg ctccattagt tgaaggtcac cgtgatgaat ggcaaaatat
      901 gcttgatatc aacatcatgg gtgtgttaaa tggaatttcc gccgtacttc caatcatgga
      961 aaaacaaaaa tctgggcata tcatttcaac tgattctgtg gcgggacatg ttgtttatcc
     1021 tgattccgcc gtttattgcg gaacaaaatt tgctgtccgt gcaatcatgg aaggattacg
     1081 tcaagagcaa cggcaaaata acatcaaatc tacgatcatc tctccaggag ctgttcaaac
     1141 tgaattatat caaaccatca gcgacaaaaa ggctgctctt gaacttcatg aagcgcaaaa
     1201 agaatgggga ttgacctccg aagatatagc ttctgctgta gcatttgcga tagaaacgcc
     1261 agaccggatg tctgtcagta acatgattat ccgtccgacg acacaggaag tataatccat
     1321 aacggataat atccaaagag aattacttgt taaatagtta ttcaaacgac catcaattta
     1381 aggcgtgaat ggagaagaat attcttcatt tacgtctttt tatgtagaaa aagcaaattt
     1441 atcaaaaaga aacaaatgtt atttaaatat ggacattgac ttgcttttac ttttttagtt
     1501 acactgttat ttaggaaaag tttcataatt caaaggagaa aaaagatgaa agcaaagaat
     1561 ttattattta gcgttattct tttaggaaat ctagcagttg ccaacacaac agtgctagct
     1621 gaagaatcga cagaaactgt cgcaactcag acatcgagtt cttcagaaac aggaattgaa
     1681 aacgatacag agagtacttt gcagtcagct actgtctcag aaaatacaat ttcaaccgaa
     1741 gaaccaacaa ctgaaacaac agaacaaaca caaaccgaag acagtacatc agcagaagct
     1801 cagaacaatc cagagaatgc agaagaaatc acgaaccaat tgacagatgg gcaaacattg
     1861 acacataccc atcaagcaga tttgtctgaa ttcgaaaact atactggcta tgctttttat
     1921 gctcgctcat ctgctaattc tcaacaagcg ttcattgata gtattgcctc aactgcacaa
     1981 agtttggcaa gtgccaatga cttatatgca tcagtcatga ttgctcaagc catcgttgaa
     2041 agtggctggg ggaacagtac tttggcttct gcccctaatt acaatctatt tgggattaaa
     2101 ggaagctata atggccaatc tgttacgatg cctacttcgg aatatgtgaa tgggcaatgg
     2161 attacggtga atgccgcttt tagaaaatat ccttcgtaca aagaatcatt gcaagataac
     2221 gtaacggtat tgaagacgac ttcttttcag ccaggcgttt actactattc cggtgcatgg
     2281 aaaagcaata cgaactctta caaagatgct acggcttggc taacaggtcg ttatgccact
     2341 gcgcctaatt atggttctac attgaacaat gtaatcgaaa cctataatct gacacagtat
     2401 gatacagcac caacttcaac ttctccgatg tatcgtctgt ataaccgtca tacaggagaa
     2461 catttatata cattgaatgc cggagaaaaa aattatctgc caactgtagg ctgggaatat
     2521 gaagggattg cttggcaagc accaaacagc ggacaacctg tctaccgttt atacaatcct
     2581 tacagtggag accatcatta cacaatggca caatctgaga tcaactcttt aacaaaaatc
     2641 ggttggcgtt atgaaggtct aagtttttat tctggtggtt ccaagccaat ttatcgacta
     2701 tttaatccaa atgaaaaaac tggtacccat cactatacct tgagtgctaa ggaaagagat
     2761 tggctaacac cgatgggctg gagatatgaa ggaataggat tttatggtta ttaaaaagag
     2821 gtagtgaaaa agctgccctg catcaagtag taagaacagc caaacaaaaa cagattttca
     2881 tgttttttgt ttggttgaac ttattaccgc agatgcaagt ttttgaacac cgtttactca
     2941 aaaaaggttg tgacaacatc ttgtcataac ttttttatta ctgtttaagc atactgatcc
     3001 aatttctaat attcgacggg attcttgcaa aaggcgattt cggaaatttt tggcggtgta
     3061 aaattttcta ctgcagaaga gctatcttct atagtttgat aagaatcaat aatcttggtt
     3121 ttagcgttgt gctttcttta tcattttttc cgttcattct acaaaaaatt tcttttctct
     3181 atacttttct attgagagcg ttttttttat tacacagctg attctttgac ttttttcttt
     3241 ttttattcta ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa ggaggaaatt
     3301 atgtctttag aagagattag tcacctttta tatcaaatca aattagccga tcaaaaaatc
     3361 gtccagatct ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga
     3421 ctgaaagaag aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa
     3481 ggagcagtta ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga
     3541 aatcctcaga acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc
     3601 gagcattgtg aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat
     3661 gtagaaatga aacaatttct aacactttta gataaattga atacccaact ttgcaatata
     3721 acaacagaaa cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat
     3781 gactggtaga agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat
     3841 gctggaaatg atcaaagaag cgacgactgc accttcttct gtcaatatgc aaccatggcg
     3901 ctttgtggtc gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa
     3961 tacgagacaa aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta
     4021 cgaatatggc gaagaaatct ataaccaagc ttacgaatca gggaagatgc caaaagaagt
     4081 cagagatcaa caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat
     4141 gaacgatgta gtcaaagtcg attctagctt agcggctatg caattcatgt tagttacgcg
     4201 tgatcatggc tatgaaacaa atccaattgg tggagtcgaa gcagatcagt tggcagaggc
     4261 atttggtttg gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt
     4321 agaagaaggt tacgagtctg ttcgtttagc accagaaaag atcactacgt tcgaataaaa
     4381 ggttttcagt tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata
     4441 aaaaaggagg tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac
     4501 aactaaataa attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt
     4561 ttttcgaacg acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt
     4621 atttcgttca gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc
     4681 ttttttagta tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat
     4741 ttagcagtaa atataacaaa tctacaagtc agctttggca accagctaga actatctatt
     4801 gattctcttc gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt
     4861 aaatcgactt tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa
     4921 acagagatca ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta
     4981 aatttggaat tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg
     5041 agcggagggg aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg
     5101 atgcttctag acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa
     5161 gagcttcgtt attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat
     5221 gagctagcat cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat
     5281 tatagtgcct atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag
     5341 tcgatcatga aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg
     5401 gaaaagttgg aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagacc ggatcggttg
     5461 tcttcctcta aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta
     5521 gagagaagac tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt
     5581 tttccagtac caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc
     5641 caattggaaa gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt
     5701 aaaaaaatcg cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc
     5761 cgcaaacaag gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa
     5821 aagggttatc aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag
     5881 ttttcagaat cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg
     5941 acgaaaccgt tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt
     6001 tttactaagc caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca
     6061 acgatcgaag ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct
     6121 catgattctt actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa
     6181 ataaaaaaag tacttacgag aaatttttaa atgtgctacg atgattttcg tgacaaattt
     6241 aaaggagtgg tacagattgc ctacaaataa aaaagaagga ataattttta caacaatgat
     6301 gtgttttttg atggtcttgg ggatgagcgc gtataattta tggctgcatc aagatcttca
     6361 tttatcaagt ttgttgattg gtctagtgcc tggattcgtg gtggctttca ttttggatgt
     6421 cttcatcatc ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa atccagaaag
     6481 caaggtccaa atgatcttaa ctatttcatg cttgatggtt ttaggaatgg tgacatgtat
     6541 gtctttattc ggtgtgatca tggaacaagg aatacctgat caattattct ctgcttatat
     6601 gaatgcttgg aaaatgaatg tgattgccgc tttaccgttg cagttgatca tcgttggccc
     6661 gctttcaaga agtgtgctaa aagcaattca aagtaatgaa tgatggaaca ctgttatttg
     6721 aaaaaaggcc ttggcatgct ttttgtcata gtctcttgaa tgcagttcta gaaaaatagt
     6781 gatccgaacg ataggttttg gcctgtcgtt cggatcgctt tttcttttcc aactgtattg
     6841 ctcttttatt gaagataatt tgctcttcaa tagatatttc tagaaaggtt ctgtttatcc
     6901 cttactcaaa aaactgctgc atttgttctc ttacttgctg acaccattgg agttcctgct
     6961 gacaaagcat aattctgcgc tggatcacaa cataacgtcc aaattcttgt ttcttgtcta
     7021 gcgagaggtc attcaatttt ttttgatttt tttgcatgat tttgttcagc tgttgttctc
     7081 tagtgaatag cagtccagta gctgttgttt tatcgagcgt cgaaaaagca tagatttttg
     7141 ctagaaattc atctttttgc gtaggagcgg gagtttcctc tttgatccat tcctctacaa
     7201 gagcacgtcc ttcatcagtc aagtggtaga tctttttttg agagtgctcg tctttgtcca
     7261 aaacggtcac ataaccctgc tggtctaact tggcaagcgt tgtatagatc tgactgtgat
     7321 gggcttccca aaaaattgtg atcagctgct tcaattcata cccagaacgt ggtttggata
     7381 aaagcacgca taataatata taggctaaag tattcataaa aatcctacct ctcgtcttat
     7441 tttctttatt atatcacagt aaagtttcca cgttttacat gtgcatattt acatgtaaaa
     7501 taaatcatgt atgatgctaa tagagagaaa gggatggaaa tattggagaa tcaaatagat
     7561 atacaagaac aaacaaatga agaaacaaag gggaaaagtt cagcgctagt tgtagcagct
     7621 acctgtatga tcgcttttat gggagtgggt ctagtagacc caatcttaaa aacgattgct
     7681 gcacagttaa acgctacgcc agctgaaacg accctgttat ttactagtta catgttagtc
     7741 actggtgtgg tgatgctatt tacaggtttc ttatctagtc ggatcggttt gaaaaaaaca
     7801 ttgatgacag gattgtttat catcgttgta ttcgctggtc ttggcggatt atctgggaat
     7861 atcggaatgc tgattgggtt acgtgccggc tggggtgtcg ggaatgcttt atttgtctct
     7921 actgctttag caacgatcgt cagtatttta gttggtaaca cagaaaaagc aatcatgatg
     7981 tatgaagcag cacttggctg cggaatggct gttggccctc tagtcggtgg ggtcttgggc
     8041 agtggttcat ggcgttatcc attttttggg gtagcatttt tgatgttttg cggttttata
     8101 gccctagcgg tacttttacc tgaaactgag aaaccaaaga gaaaagtagg cctttgggaa
     8161 ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg gactaatcgc attgttatat
     8221 aatttcggat tctttacttt acttgcgtat gcgccatttt tattgattgg ctattctgaa
     8281 ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc tggctattgc ttcgattttc
     8341 gttgcaccgg ctcttgagcg gaagtttacg acttatcgga caattttagc agcatttgtc
     8401 ttattcatta tttgtttgct gttattagga gctggaactt ctatgccagt ccttgtaccg
     8461 atcagcattg tgttagcagg atttttccaa ggaattatca atacactgtt gacgacgatt
     8521 gctatggaaa taccaggtct gcaacgaaac gttgcttcgt ccagctatag ttttgtccgc
     8581 tttttcggtg gagctttagc accatttatt gcaggtgtga ttggtgaaca tattggtgaa
     8641 agctatgcgt tttattttgc aggtgccatt gttcttatca gtgtatgttg tatcatttat
     8701 caccgtaatt attttgctac aaatgaagaa gaataagaaa tcagaggttg tgaaaaagct
     8761 gtcctgcatc aagtcataag aa
//
LOCUS       DACJGB010000239         6571 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.118, whole genome shotgun sequence.
ACCESSION   DACJGB010000239 DACJGB010000000
VERSION     DACJGB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.118"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            complement(75..1367)
                     /locus_tag="IYI34_003220"
     CDS             complement(75..1367)
                     /locus_tag="IYI34_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAR0560999.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            1699..2667
                     /locus_tag="IYI34_003221"
     CDS             1699..2667
                     /locus_tag="IYI34_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAR0561000.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            2660..3691
                     /gene="vanA"
                     /locus_tag="IYI34_003222"
     CDS             2660..3691
                     /gene="vanA"
                     /locus_tag="IYI34_003222"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAR0561001.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            3697..4305
                     /locus_tag="IYI34_003223"
     CDS             3697..4305
                     /locus_tag="IYI34_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAR0561002.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            4733..5644
                     /gene="vanY"
                     /locus_tag="IYI34_003224"
     CDS             4733..5644
                     /gene="vanY"
                     /locus_tag="IYI34_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAR0561003.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            5797..6282
                     /gene="vanZ"
                     /locus_tag="IYI34_003225"
     CDS             5797..6282
                     /gene="vanZ"
                     /locus_tag="IYI34_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAR0561004.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT         2053 a         1139 c         1437 g         1942 t
ORIGIN      
        1 ttgtcaaatg atgttggtaa agagaaatct tagaataatt ggagatcaag tggatcagtt
       61 cctcttgatc ttttttagtt gaaaatcaat gcgttgccat gacgaatcat gatgcgcaat
      121 ttgaaattat aaaagctacg aaagccatat gcatttcgtt ttaacacttt gatatggttg
      181 ttcagacatt caagtgggcc atttgaataa ctttgttgta aagcattttt gatttgtggc
      241 agaaatttct ggaacgttct caaggtcgtc tgatagcctt cgggaagcat gatattattg
      301 gtaagtagtt cttcaaacga tacgtagtct cgttccttta cagcgtaaag aaagtcttga
      361 tataactgat agcctacacg caaagcaggg ctccctttca gtaagcgatc cacaatatcg
      421 gtctctgtta aatgagcttt gaagccaggt cgccaaaggc gtcgagtata atcaagcttg
      481 cgttcatctt tttgaagaag tttccagtat ctttttaatt gcttacctaa ttgatagtgt
      541 ttttgttctt tgctttttaa tagttggttc gtttctttga ttcgatgatt gcgaaaggta
      601 cgaccaatat gttggacgat gtgaaaccgg tcaataatca gctgtgcttt tggaaacagc
      661 tttttaacga gcgatacata aggagcgtac atatcaatca cgatccattc gaccgcttca
      721 cgaatttctc gtgaaaaatg actaaaataa cgttttaaaa aaggcagttg tcggttctcg
      781 acaatgtcca ataagcgctg tgtttgtcca tccatcatga tgaaactcat agcacctgaa
      841 acaccccgaa ctgatttgaa ttcatcaaaa caaagtacct taggtaacgg tgtccgaaaa
      901 ggattcaacg gacgataaaa tcgcttcata atacggtaaa cagaagagac cgatagattt
      961 ttctgagcgg cgatctctgt cattgaggta ttttttttca gacgagtcac gacagaccag
     1021 cggactttct cactgatgaa acaatgtttt ttcgcaacag aagtatctgc gacaaaggtc
     1081 cgctgacaag ctttgcattt gaagcgttgc tttttcaatt cgaggtaggt acggtattca
     1141 gaaacatcgt tcattaacaa ccggacggtt gttgtacccc aacgcacaat atctgaagca
     1201 atctggtgtt tacagtggat gcattcttgg gctgtataaa tcaagtttcc acgataaata
     1261 tgacagattt gccctttgta tttttctttt gtgagacaat tttcgttaaa agtaactgat
     1321 aagtctagaa tatctaatgt ttctttgata aggtgagtgt aagacatctt gattcctcct
     1381 tttgggttgg ttcgccaaat taattctaaa ggatttcaag gtgtcttttc tatatgtttt
     1441 ggaaaataaa aaagatgctg gtgtcatcca ccaacatcaa aaagtataga gccataattt
     1501 tttaggaaaa tctcaaggtt atctttactt tttcttagga aattaacaat ttaatattaa
     1561 gaaacggctc gttcttacac ggtagactta ataccgtaag aacgagccgt tttcgttctt
     1621 cagagaaaga tttgacaaga ttaccattgg catccccgtt ttatttggtg cctttcacag
     1681 aaagggttgg tcttaattat gaataacatc ggcattactg tttatggatg tgagcaggat
     1741 gaggcagatg cattccatgc tctttcgcct cgctttggcg ttatggcaac gataattaac
     1801 gccaacgtgt cggaatccaa cgccaaatcc gcgcctttca atcaatgtat cagtgtggga
     1861 cataaatcag agatttccgc ctctattctt cttgcgctga agagagccgg tgtgaaatat
     1921 atttctaccc gaagcatcgg ctgcaatcat atagatacaa ctgctgctaa gagaatgggc
     1981 atcactgtcg acaatgtggc gtactcgccg gatagcgttg ccgattatac tatgatgcta
     2041 attcttatgg cagtacgcaa cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc
     2101 aggttggaca gcgaccgtgg caaggtactc agcgacatga cagttggtgt ggtgggaacg
     2161 ggccagatag gcaaagcggt tattgagcgg ctgcgaggat ttggatgtaa agtgttggct
     2221 tatagtcgca gccgaagtat agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat
     2281 agcgatatcg ttacgcttca tgtgccgctc aatacggata cgcactatat tatcagccac
     2341 gaacaaatac agagaatgaa gcaaggagca tttcttatca atactgggcg cggtccactt
     2401 gtagatacct atgagttggt taaagcatta gaaaacggga aactgggcgg tgccgcattg
     2461 gatgtattgg aaggagagga agagtttttc tactctgatt gcacccaaaa accaattgat
     2521 aatcaatttt tacttaaact tcaaagaatg cctaacgtga taatcacacc gcatacggcc
     2581 tattataccg agcaagcgtt gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat
     2641 tttgaaagga gacaggagca tgaatagaat aaaagttgca atactgtttg ggggttgctc
     2701 agaggagcat gacgtatcgg taaaatctgc aatagagata gccgctaaca ttaataaaga
     2761 aaaatacgag ccgttataca ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa
     2821 accttgcgcg gaatgggaaa acgacaattg ctattcagct gtactctcgc cggataaaaa
     2881 aatgcacgga ttacttgtta aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc
     2941 attttcagct ttgcatggca agtcaggtga agatggatcc atacaaggtc tgtttgaatt
     3001 gtccggtatc ccttttgtag gctgcgatat tcaaagctca gcaatttgta tggacaaatc
     3061 gttgacatac atcgttgcga aaaatgctgg gatagctact cccgcctttt gggttattaa
     3121 taaagatgat aggccggtgg cagctacgtt tacctatcct gtttttgtta agccggcgcg
     3181 ttcaggctca tccttcggtg tgaaaaaagt caatagcgcg gacgaattgg actacgcaat
     3241 tgaatcggca agacaatatg acagcaaaat cttaattgag caggctgttt cgggctgtga
     3301 ggtcggttgt gcggtattgg gaaacagtgc cgcgttagct gttggcgagg tggaccaaat
     3361 caggctgcag tacggaatct ttcgtattca tcaggaagtc gagccggaaa aaggctctga
     3421 aaacgcagtt ataaccgttc ccgcagacct ttcagcagag gagcgaggac ggatacagga
     3481 aacggcaaaa aaaatatata aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt
     3541 tttacaagat aacggccgca ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc
     3601 atacagtcgt tatccccgta tgatggccgc tgcaggtatt gcacttcccg aactgattga
     3661 ccgcttgatc gtattagcgt taaaggggtg ataagcatgg aaataggatt tactttttta
     3721 gatgaaatag tacacggtgt tcgttgggac gctaaatatg ccacttggga taatttcacc
     3781 ggaaaaccgg ttgacggtta tgaagtaaat cgcattgtag ggacatacga gttggctgaa
     3841 tcgcttttga aggcaaaaga actggctgct acccaagggt acggattgct tctatgggac
     3901 ggttaccgtc ctaatcgtgc tgtaaactgt tttatgcaat gggctgcaca gccggaaaat
     3961 aacctgacaa aggaaagtta ttatcccaat attgaccgaa ctgagatgat ttcaaaagga
     4021 tacgtggctt caaaatcaag ccatagccgc ggcagtgcca ttgatcttac gctttatcga
     4081 ttagacacgg gtgagcttgt accaatgggg agccgatttg attttatgga tgaacgctct
     4141 catcatgcgg caaatggaat atcatgcaat gaagcgcaaa atcgcagacg tttgcgctcc
     4201 atcatggaaa acagtgggtt tgaagcatat agcctcgaat ggtggcacta tgtattaaga
     4261 gacgaaccat accccaatag ctattttgat ttccccgtta aataaacttt taaccgttgc
     4321 acggacaaac tatataagct aactctttcg gcaggaaacc cgacgtatgt aactggttct
     4381 tagggaattt atatatagta gatagtattg aagatgtaag gcagagcgat attgcggtca
     4441 ttatctgcgt gcgctgcggc aagatagcct gataataaga ctgatcgcat agaggggtgg
     4501 tatttcacac cgcccattgt caacaggcag ttcagcctcg ttaaattcag catgggtatc
     4561 acttatgaaa attcatctac attggtgata atagtaaatc cagtagggcg aaataattga
     4621 ctgtaattta cggggcaaaa cggcacaatc tcaaacgaga ttgtgccgtt taaggggaag
     4681 attctagaaa tatttcatac ttccaactat atagttaagg aggagactga aaatgaagaa
     4741 gttgtttttt ttattgttat tgttattctt aatatactta ggttatgact acgttaatga
     4801 agcactgttt tctcaggaaa aagtcgaatt tcaaaattat gatcaaaatc ccaaagaaca
     4861 tttagaaaat agtgggactt ctgaaaatac ccaagagaaa acaattacag aagaacaggt
     4921 ttatcaagga aatctgctat taatcaatag taaatatcct gttcgccaag aaagtgtgaa
     4981 gtcagatatc gtgaatttat ctaaacatga cgaattaata aatggatacg ggttgcttga
     5041 tagtaatatt tatatgtcaa aagaaatagc acaaaaattt tcagagatgg tcaatgatgc
     5101 tgtaaagggt ggcgttagtc attttattat taatagtggc tatcgagact ttgatgagca
     5161 aagtgtgctt taccaagaaa tgggggctga gtatgcctta ccagcaggtt atagtgagca
     5221 taattcaggt ttatcactag atgtaggatc aagcttgacg aaaatggaac gagcccctga
     5281 aggaaagtgg atagaagaaa atgcttggaa atacgggttc attttacgtt atccagagga
     5341 caaaacagag ttaacaggaa ttcaatatga actatggcat attcgctatg ttggtttacc
     5401 acatagtgcg attatgaaag aaaagaattt cgttctcgag gaatatatgg attacctaaa
     5461 agaagaaaaa accatttctg ttagtgtaaa tggggaaaaa tatgagatct tttattatcc
     5521 tgttactaaa aataccacca ttcatgtgcc gactaatctt cgttatgaga tatcaggaaa
     5581 caatatagac ggtgtaattg tgacagtgtt tcccggatca acacatacta attcaaggag
     5641 gtaaggatgg cggaatgaaa ccaacgaaat taatgaacag cattattgta ctagcacttt
     5701 tggggtaacg ttagcttttt aatttaaaac ccacgttaac taggacattg ctatactaat
     5761 gatacaactt aaacaaaaga attagaggaa attatattgg gaaaaatatt atctagagga
     5821 ttgctagctt tatatttagt gacactaatc tggttagtgt tattcaaatt acaatacaat
     5881 attttatcag tatttaatta tcatcaaaga agtcttaact tgactccatt tactgctact
     5941 gggaatttca gagagatgat agataatgtt ataatcttta ttccatttgg cttgcttttg
     6001 aatgtcaatt ttaaagaaat cggattttta cctaagtttg cttttgtact ggttttaagt
     6061 cttacttttg aaataattca atttatcttc gctattggag cgacagacat aacagatgta
     6121 attacaaata ctgttggagg ctttcttgga ctgaaattat atggtttaag caataagcat
     6181 atgaatcaaa aaaaattaga cagagttatt atttttgtag gtatactttt gctcgtatta
     6241 ttgctcgttt accgtaccca tttaagaata aattacgtgt aagatgtcta aatcaagcaa
     6301 tctgatcttt catacacata aagatattga atgaattgga ttagatggaa aacgggatgt
     6361 ggggaaactc gcccgtaggt gtgaagtgag gggaaaaccg gtgataaagt aaaaagctta
     6421 cctaacacta tagtaacaaa gaaagcccaa ttatcaattt tagtgctgag gaattggtct
     6481 ctttaataaa tttccttaac gttgtaaatc cgcattttcc tgacggtacc cccaaactaa
     6541 gatttcatag atagattttc tgataacttt a
//
LOCUS       DACJGB010000240         4740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.141, whole genome shotgun sequence.
ACCESSION   DACJGB010000240 DACJGB010000000
VERSION     DACJGB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.141"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..982
                     /locus_tag="IYI34_003226"
     CDS             <1..982
                     /locus_tag="IYI34_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAR0561005.1"
                     /translation="GSGKSVAINGIITSILMRAKPHEVKLMMIDPKMVELNMYNGIPH
                     LLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQQKNLENGTKHPT
                     LPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQRPSVDVITGIIKA
                     NVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIRVQGAFISDHEVE
                     RVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVVEMQTASISLLQR
                     RFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPDDNDM"
     gene            1212..2279
                     /gene="rlmN"
                     /locus_tag="IYI34_003227"
     CDS             1212..2279
                     /gene="rlmN"
                     /locus_tag="IYI34_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAR0561006.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(2472..3575)
                     /locus_tag="IYI34_003228"
     CDS             complement(2472..3575)
                     /locus_tag="IYI34_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAR0561007.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            4127..4675
                     /gene="aac(6')"
                     /locus_tag="IYI34_003229"
     CDS             4127..4675
                     /gene="aac(6')"
                     /locus_tag="IYI34_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAR0561008.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1569 a          813 c          998 g         1360 t
ORIGIN      
        1 tggtagtggg aagtcagtag caattaatgg gattatcaca agtatattaa tgcgtgctaa
       61 gccccatgaa gtaaaactga tgatgatcga tccgaaaatg gttgaattga atatgtacaa
      121 cggcatacct catctgttga cgccggtcgt gacgaaccct agaaaagcag cgcaagcctt
      181 acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa ttcgcggcaa ccggagtaag
      241 aaatatttct ggttacaatg aatttgttca acagaaaaac ttggaaaacg gaacgaaaca
      301 tccaacctta ccattcatcg tagtgatcgt agatgaatta gctgatttga tgatggtagc
      361 cagcaatgaa gttgaagatg cgattattcg tttagcgcaa atggctcggg cagcggggat
      421 tcatatgatc ctagctactc aacgtccaag tgtggatgtt atcacaggaa tcatcaaagc
      481 aaatgtacct tccagaatgg catttgcggt atcaagcggt acagattctc ggacgatcat
      541 tgattctaat ggggcagaaa agctcttagg gcgaggagat atgctattct tgccaatggg
      601 tgagaataaa ccgatccgtg tgcaaggagc attcatttct gaccacgaag tagaacgggt
      661 cgttcaattt gttacagatc agcaagaagc tcattatgaa gagaaaatga tgccgacaga
      721 tgaagttgaa acgacaggtg ctccagaaca gccacaagat gagctgtttg aagaagcaaa
      781 agcacttgtc gttgaaatgc aaacggcaag tatttccctt ctccaaagaa gatttcggat
      841 cggttacaac cgagcagcac gtttagtaga tgaactggaa gctcatggag ttgttggccc
      901 gtcagaagga agcaagcctc ggaaagtatt cattgagcag gaagaaacat cagaaacgtt
      961 gccggacgat aatgacatgt aaaacttgtg tgatgaaggg ttgaaaaacg aataaccaat
     1021 aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt tttttccatc ttttctaata
     1081 cggtatgatg tcttgtcttt ttagtttttg tgagattaaa gttctattcg atttatttgt
     1141 gaagtattgc attaaggaaa tgaatcatgt atagtatgta gcgaaacttt gagaaaaaga
     1201 gggaaaatac tttggaaaaa cagtccattt atggcttgac gaatgaagaa ttaatcaatt
     1261 ggtttataga aaacggcgag aaaaaattcc gagctgccca agtttgggaa tggttgtatc
     1321 aaaaacgtgt aagtaatttc acagaaatga cgaatctatc gaaacaattg atcgaaaaat
     1381 tatcggagca ttttatcatc aatcctctaa aacagatggt cgtccaagaa gcttcggatg
     1441 gtacagtaaa atatttgttt gaactccctg ataaaaacat gattgagact gtactgatga
     1501 gacaagaata tggcttgtca gtttgtgtga cgacacaagt aggatgtaat atcggctgta
     1561 cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct gactgctgga gaaattgttg
     1621 cacagatcat gatggtccaa cattattttg atgagcgcaa tcttggcgaa cgcgtttcac
     1681 atgtcgttgt aatggggatc ggtgagccat ttgataatta cgacaatgta atggactttt
     1741 tgcatatcat caatgatgcc aaaggattgg caattggtgc tcggcacatt acagtttcca
     1801 ctagtggatt agcacacaaa atcaaggaat tcgctaataa cggcttgcaa gtcaatctgg
     1861 ctatttcctt gcacgcgccg aataatgagg tgcgaacatc gatcatgcgg atcaaccgaa
     1921 gtttcccaat tgagaaattg atggaagctg tggatgagta tttagagaaa acaaaccgtc
     1981 gaatcacgtt tgaatatatc atgttgaatc aagtaaatga tagaccagag catgcgcaac
     2041 aattagcaga tctattaaaa gataagaaaa aattagcata tgtcaacttg attccatata
     2101 atccagtcag tgaacatgat caatattctc gcagtccaaa agctgatgta ttgagatttt
     2161 atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg taaagaacat ggtacagata
     2221 ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa aaaaacagtt gccaaataat
     2281 cgtatcctac tcgttttttc aattaccata aaaggagcag ttttcttaat taaggaactg
     2341 ttcaacttaa ataagagaaa agattctata gcgaaagcta taaagctttt tttgacaaga
     2401 aaaaccctca tcaagcaaag aaatgtcctc aatttgatga cagcctgctt gatgggggtt
     2461 ttagcaatta tttaactgat tttcttatat ccttgctggc tttcttgtgt agcatgataa
     2521 gtttctacgt caagaatggc ctgtttaaat ttttctcggg tgatttcata aggcacatgt
     2581 ttaatatcat ttgttgccat ggctttatct aagattgctt ctaaatcggt cgtgacattg
     2641 agattcatgt cggttaacga tactggaagt tggttctttt tcataaacac atagatttta
     2701 tcaaacaaat cgaactgctg atccatcatc aataaggtca gtacaccata actgacagat
     2761 gctccgtgaa ggtatccctc cattttttct agaacagtcg taccgtaata gaatgagtgt
     2821 gccatagcag aattatagtt attgttaact aaaacagaaa ccaatccggt agtaacaatg
     2881 atatccgaaa cgacttgttc aattgcatat gagggacgat cgtcggcact atctttcaat
     2941 gcttgcgtac cataagtcaa gagacgatca atgcacattt ttccgatttg gattcctaat
     3001 ctatccgtgt agcctagatc atcgccacga gcagaaaaag tagattcgac ttctttggat
     3061 aaagcatcgc caatacctgc ccataagtat ttatcaggtg cttcggcaat aattgctgta
     3121 tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga gtccgtccat tacgccttct
     3181 tcgttataga tgacacatac cgcagtgacg ggcgaacagt tcgaaccaat agtgggaaag
     3241 gtgaagactg gtttagctag tttttcacca atgactttta cagtatcgca agcacggcca
     3301 ccaccgactg caaacaggac gtctgctttt tggacacttt catgctggat caactgttca
     3361 atatttccat aagttgaatt acccccgtac cagagactgg cgacaacctc tatttcctgt
     3421 gtcaaagcta cttttatttt gttttctgcc tttgaaagtg cagtcttccc tccaatcagc
     3481 actacttttg tcccatattc tttgacgatt ccatttgctt tttcatacgc atctgttcca
     3541 atggtataat ttggtaagaa aacactttga ctcatattca tttccttctt tcttcgttga
     3601 aacgggttaa aattttaaag agtgatcgat ctgactgcgg ctgtagatgg agtagaggtg
     3661 aacctaagac aggataagtg atgaataagg tggcatgaac accttgaaat catcaataag
     3721 tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac aataatgaat ttttgttcct
     3781 cttagtattt ctaccgattc cctttgcttt tttgtaatta tctaaaaatt ctaaaaatta
     3841 agattattat atgaaaacag gttttatttg tcaattcata attggttttt tttaaagcaa
     3901 aagataatct atatcatgat gaacaaacag agttttttcc tccaattgat ggctttcctg
     3961 atctttccta ttttgaataa agtgtttctg tataattttt tattttataa gtaagataaa
     4021 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
     4081 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
     4141 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
     4201 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
     4261 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
     4321 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
     4381 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
     4441 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
     4501 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
     4561 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
     4621 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
     4681 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DACJGB010000241         3950 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.156, whole genome shotgun sequence.
ACCESSION   DACJGB010000241 DACJGB010000000
VERSION     DACJGB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.156"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            56..790
                     /locus_tag="IYI34_003230"
     CDS             56..790
                     /locus_tag="IYI34_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0561009.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            823..1731
                     /locus_tag="IYI34_003231"
     CDS             823..1731
                     /locus_tag="IYI34_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAR0561010.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1728..2270
                     /gene="sat4"
                     /locus_tag="IYI34_003232"
     CDS             1728..2270
                     /gene="sat4"
                     /locus_tag="IYI34_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAR0561011.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2363..3157
                     /locus_tag="IYI34_003233"
                     /note="aph(3')-IIIa"
     CDS             2363..3157
                     /locus_tag="IYI34_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAR0561012.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(3265..3453)
                     /locus_tag="IYI34_003234"
                     /pseudo
     CDS             complement(3265..3453)
                     /locus_tag="IYI34_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3433..>3950
                     /locus_tag="IYI34_003235"
     CDS             3433..>3950
                     /locus_tag="IYI34_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0561013.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEP"
BASE COUNT         1351 a          600 c          919 g         1080 t
ORIGIN      
        1 gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag ttcagatgaa
       61 agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga gtcgctcgca
      121 gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac ctgattttaa
      181 gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga
      241 aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa
      301 aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt
      361 cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta
      421 tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac
      481 agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg
      541 agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt
      601 tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct
      661 ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga
      721 tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa
      781 gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa
      841 atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat tgtgaccctt
      901 gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta tgatattaca
      961 tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa tcaatttggg
     1021 aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga agaaaaggga
     1081 ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt attgcccttg
     1141 gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat tgataaagat
     1201 tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag aaagccaagc
     1261 gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta tgttattaaa
     1321 ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat tgttcgccat
     1381 gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa attaagtgta
     1441 ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga gaaacttttg
     1501 tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct atgccatcaa
     1561 ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta tccggagtat
     1621 gataggaata taacaaaata taccagggac atgtataaaa aatacactgg taaaaccggc
     1681 tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg attacagaaa
     1741 tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag gtgataggta
     1801 agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc tatgaagcgc
     1861 catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca gattgccttg
     1921 aatatattga caatactgat aagataatat atctttacta ccaagacgat aaatgcgtcg
     1981 gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat atcgccgtat
     2041 gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg
     2101 caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt
     2161 gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     2221 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     2281 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     2341 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     2401 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     2461 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     2521 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     2581 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     2641 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     2701 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     2761 gaatagctta gacagccgat tagccgaatt ggattactta ctgaataacg atctggccga
     2821 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     2881 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     2941 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     3001 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     3061 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     3121 aataaaatat tatattttac tggatgaatt gttttagtac ctagatttag atgtctaaaa
     3181 agctttaact acaagctttt tagacatcta atcttttctg aagtacatcc gcaactgtcc
     3241 atactctgat gttttatatc ttttctaaaa gttcgctaga tagagttcta tattagagat
     3301 gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt ataaccaggg tgcaatgaga
     3361 agcactaaca gcagctagct taagaacgca taggatatac tttttatgga agtcacagag
     3421 gaggaataaa atttgaacag gattaacaga gtaacctcta ttcttattca gctgcaatca
     3481 aaaaaaataa ttcctgccaa agaaattgca cagcgattta atataagctt aaggacagtt
     3541 tacagagata tccggacact tgaagaagcc ggaataccaa ttggatctga ggccggaaaa
     3601 ggatattttc ttgtagaggg cttcctactt ccgccggtaa tgtttacagc ggcggaagtg
     3661 ggtgcattga ttacagcagg gaaattttta aattgccatg gagatgaatc atttataaag
     3721 gattttgatt cagctatgta taaaatcaaa tctattttga agcatggtga aaaaaactat
     3781 gcacaggagt tggagaacag tattaacgtg tacagcacat ctggacagaa aaatacattg
     3841 gcggataacg ttattgcagc aattcagact gcaatctgca ataaaagggt aatatcaatt
     3901 caatatcctg catcaggagg gcaagaaccg gaaagcagaa tgatagagcc
//
LOCUS       DACJGB010000242         3950 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.157, whole genome shotgun sequence.
ACCESSION   DACJGB010000242 DACJGB010000000
VERSION     DACJGB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.157"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            56..790
                     /locus_tag="IYI34_003236"
     CDS             56..790
                     /locus_tag="IYI34_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAR0561014.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            823..1731
                     /locus_tag="IYI34_003237"
     CDS             823..1731
                     /locus_tag="IYI34_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAR0561015.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1728..2270
                     /gene="sat4"
                     /locus_tag="IYI34_003238"
     CDS             1728..2270
                     /gene="sat4"
                     /locus_tag="IYI34_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HAR0561016.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2363..3157
                     /locus_tag="IYI34_003239"
                     /note="aph(3')-IIIa"
     CDS             2363..3157
                     /locus_tag="IYI34_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAR0561017.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(3265..3453)
                     /locus_tag="IYI34_003240"
                     /pseudo
     CDS             complement(3265..3453)
                     /locus_tag="IYI34_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3433..>3950
                     /locus_tag="IYI34_003241"
     CDS             3433..>3950
                     /locus_tag="IYI34_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAR0561018.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEP"
BASE COUNT         1350 a          601 c          919 g         1080 t
ORIGIN      
        1 gaagtgaatg aaattgtatc ggagataaat aactttttaa acgaggagag ttcagatgaa
       61 agaaaacaaa tatgatgata atatattttt tcaaaaatac agtcaaatga gtcgctcgca
      121 gaaaggactg gctggtgcgg gagaatggga gactttgaaa aagatgctac ctgattttaa
      181 gggtaagcgt gtgcttgatt taggatgcgg ctatggatgg cactgtatat atgcgatgga
      241 aaacggtgct tcctctgtag taggtgttga tatttctcat aaaatgctcg aagtagcaaa
      301 aggaaaaacc cattttccac agattgaata tgaatgctgt gccatagaag atgtggattt
      361 cccagaggag agctttgatg taatactaag ttcgcttgcg tttcattatg tagcagacta
      421 tgagaattta ataaaaaaga tatataggat gctgaaggct ggtggcaatt tagtttttac
      481 agttgaacat cctgttttta ctgctcatgg aacacaagac tggtattata acgaaaaagg
      541 agaaatactg catttcccgg tggacaatta ttattatgag ggcaaacgga cagctatgtt
      601 tttggaagaa aaggttacaa aatatcatag aacactgacc acatatctaa atacactgct
      661 ttcaaatagt tttataataa atcagattgt ggagccacag ccgccagaga acatgatgga
      721 tattccgggg atggcggatg aaatgcgacg cccaatgatg ctgattgtat cggcaaaaaa
      781 gaagatgtaa taatatagaa aaaataaacg aggagtatgt aaatgagatc agaaaaagaa
      841 atgatggatt tagtactttc tttagcagaa caggatgaac gtattcgaat tgtgaccctt
      901 gaggggtcac gcgcaaatat taatatacct aaagatgaat ttcaggatta tgatattaca
      961 tattttgtaa gtgatataga accgtttata tctaatgatg actggcttaa tcaatttggg
     1021 aatataataa tgatgcaaaa gccggaggat atggaattat tcccacctga agaaaaggga
     1081 ttttcctatc ttatgctatt tgatgattac aataaaattg atcttacctt attgcccttg
     1141 gaagagttag ataattacct aaagggcgat aaattaataa aggttctaat tgataaagat
     1201 tgtagaatta aaagggacat agttccgact gatatagatt atcatgtaag aaagccaagc
     1261 gcaagggagt atgatgattg ctgcaatgaa ttttggaatg taacacctta tgttattaaa
     1321 ggattgtgcc gtaaggaaat tttatttgct attgatcatt ttaatcagat tgttcgccat
     1381 gagctgctga gaatgatatc atggaaggtc ggcatcgaaa caggctttaa attaagtgta
     1441 ggcaagaact ataagtttat tgaaaggtat atatccgagg atttgtggga gaaacttttg
     1501 tccacctacc ggatggattc ctatgaaaac atatgggaag cattatttct atgccatcaa
     1561 ttgttcaggg cggtatccgg tgaggtggcg gaaaggcttc attatgccta tccggagtat
     1621 gataggaata taacaaaata taccagggac atgtataaaa aatacactgg taaaaccggc
     1681 tgcctggata gcacatatgc cgctgatata gaagagaggc gggaacagtg attacagaaa
     1741 tgaaagcagg gcacctgaaa gatatcgata aacccagcga accatttgag gtgataggta
     1801 agattatacc gaggtatgaa aacgagaatt ggacctttac agaattactc tatgaagcgc
     1861 catatttaaa aagctaccaa gacgaagagg atgaagagga tgaggaggca gattgccttg
     1921 aatatattga caatactgat aagataatat atctttacta ccaagacgat aaatgcgtcg
     1981 gaaaagttaa actgcgaaaa aattggaacc ggtacgctta tatagaagat atcgccgtat
     2041 gtaaggattt cagggggcaa ggcataggca gcgcgcttat caatatatct atagaatggg
     2101 caaagcataa aaacttgcat ggactaatgc ttgaaaccca ggacaataac cttatagctt
     2161 gtaaattcta tcataattgt ggtttcaaaa tcggctccgt cgatactatg ttatacgcca
     2221 actttgaaaa caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga
     2281 acagtgaatt ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta
     2341 gaaaagagga aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact
     2401 gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa
     2461 gctggtggga gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac
     2521 cacctatgat gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt
     2581 tccaaaggtc ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc
     2641 cgatggcgtc ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga
     2701 gctgtatgcg gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac
     2761 gaatagctta gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga
     2821 tgtggattgc gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga
     2881 ttttttaaag acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga
     2941 cagcaacatc tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg
     3001 cagggcggac aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg
     3061 ggaagaacag tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa
     3121 aataaaatat tatattttac tggatgaatt gttttagtac ctagatttag atgtctaaaa
     3181 agctttaact acaagctttt tagacatcta atcttttctg aagtacatcc gcaactgtcc
     3241 atactctgat gttttatatc ttttctaaaa gttcgctaga tagagttcta tattagagat
     3301 gtaaagagtt ttggtgaaga ggtttgtgag tatggctttt ataaccaggg tgcaatgaga
     3361 agcactaaca gcagctagct taagaacgca taggatatac tttttatgga agtcacagag
     3421 gaggaataaa atttgaacag gattaacaga gtaacctcta ttcttattca gctgcaatca
     3481 aaaaaaataa ttcctgccaa agaaattgca cagcgattta atataagctt aaggacagtt
     3541 tacagagata tccggacact tgaagaagcc ggaataccaa ttggatctga ggccggaaaa
     3601 ggatattttc ttgtagaggg cttcctactt ccgccggtaa tgtttacagc ggcggaagtg
     3661 ggtgcattga ttacagcagg gaaattttta aattgccatg gagatgaatc atttataaag
     3721 gattttgatt cagctatgta taaaatcaaa tctattttga agcatggtga aaaaaactat
     3781 gcacaggagt tggagaacag tattaacgtg tacagcacat ctggacagaa aaatacattg
     3841 gcggataacg ttattgcagc aattcagact gcaatctgca ataaaagggt aatatcaatt
     3901 caatatcctg catcaggagg gcaagaaccg gaaagcagaa tgatagagcc
//
LOCUS       DACJGB010000243         3025 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.169, whole genome shotgun sequence.
ACCESSION   DACJGB010000243 DACJGB010000000
VERSION     DACJGB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.169"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            <1..1311
                     /locus_tag="IYI34_003242"
     CDS             <1..1311
                     /locus_tag="IYI34_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAR0561019.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
     gene            complement(1441..2613)
                     /locus_tag="IYI34_003243"
     CDS             complement(1441..2613)
                     /locus_tag="IYI34_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAR0561020.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1059 a          389 c          538 g         1039 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg agtcaagtcc agactcctgt gtaaaatgct atacaatgtt tttaccattt
     1441 ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat gtaggtccat
     1501 aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc gaataatctt
     1561 ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct ttagtcgatt
     1621 gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat ccaatgatgc
     1681 gcaagctttt gaatattttg gttgatcgat ataatcatga atcaatcgat ttttagcctc
     1741 acgcgctaag ttaatatctg tgaacttaaa aattccttta acagcttctc tgaaagattt
     1801 tgaatttttt ttaggaatgg tggtaaagat atttcttagg aagtgaactt ggcatctttg
     1861 ccaacttacg ttggtgaagg attttctaat ggcagagact aatcctttgt gcgcatcaga
     1921 aataacgagt tccgtacctt gtaaaccgcg ttcttttagg tattcaaaaa atgttgtcca
     1981 ggtctcttcg ctttcgccac tttgaatcat gaagccgata atttcacggt cgccatcttt
     2041 ggttattcca atcgctatat gacagctttt tgagagtact cgattttctt ctcgtacttt
     2101 tatatagagt acatcggtca ttaagtaagg ataatttttt tctgataata aacgattctg
     2161 ccactcgtta accataggtt ctagctgttc tgttaagcta gaaacgaagg acttagagac
     2221 ggatttacca caaagttctt ccacaatttt tgatacttta cgagttgaaa cgcctgatac
     2281 atacatttcc aacattgaag ccatgagggc tttttcgttt cgttgataac gttcaaacac
     2341 tgtgggtgaa aaatggccat cacgtgttct gggtactttt aattctagcg tgcctacacg
     2401 tgtcgtaaag ctgcgctcat aatagccatt tcgttgactt tgtcggtttt ctgttcgttc
     2461 atattctttt gcttgaatat attctgttcg ttgattttcc attagttgat taaataccgt
     2521 tgttaaaata tttttagaaa cgtcatcctt tacagaatat tcaataatgc tttgaatctc
     2581 ttcgcttttc agtgtaaaat gtacttgggt catgtaaaag tcctcctggg tatgtttttg
     2641 tcgttaaaaa cattgtaccg taaaaggact gttatatggc ctttttactt ttacacaatt
     2701 atacggactt tatctatata aaggcgctgt cagagttttt tgacttagaa catacattca
     2761 ttcaagagac tttaaacaag ctagcagaac gcccaaaaac ggacacacaa ctcgatttgt
     2821 ttagctatga tacaggctga aaataaaacc cgcactatgc cattacattt atatctatga
     2881 tacgtgtttg tttttctgta ctgtttcctg agtgattagc agaaatataa agattaaaat
     2941 tttaattgat tattaggaag agaaggagaa tgtatcccga aaacttttta gttggcttgg
     3001 actgaacgaa gtgagggaaa ggcta
//
LOCUS       DACJGB010000244         2544 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.175, whole genome shotgun sequence.
ACCESSION   DACJGB010000244 DACJGB010000000
VERSION     DACJGB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.175"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            322..1014
                     /locus_tag="IYI34_003244"
     CDS             322..1014
                     /locus_tag="IYI34_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAR0561021.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="IYI34_003245"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="IYI34_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAR0561022.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..>2544)
                     /locus_tag="IYI34_003246"
     CDS             complement(1910..>2544)
                     /locus_tag="IYI34_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAR0561023.1"
                     /translation="ELRLSDAHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFK
                     TKFLRTLASHYLTELLTSHQFHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCR
                     TDLSALEPNEIAKMLLNVNDHSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNP
                     KYAQFAITILRTYYNFCRPFKSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          959 a          351 c          454 g          780 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctt
//
LOCUS       DACJGB010000245         2192 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.182, whole genome shotgun sequence.
ACCESSION   DACJGB010000245 DACJGB010000000
VERSION     DACJGB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.182"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            310..1005
                     /gene="vanR"
                     /locus_tag="IYI34_003247"
     CDS             310..1005
                     /gene="vanR"
                     /locus_tag="IYI34_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAR0561024.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            983..2137
                     /locus_tag="IYI34_003248"
     CDS             983..2137
                     /locus_tag="IYI34_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAR0561025.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          735 a          405 c          465 g          587 t
ORIGIN      
        1 tatttgatag tagatttaaa actttgcaac agaaccgaaa ttactaatgt atctagggct
       61 tcattataca ggaaattatc agaagtgaat aattagccat tctgtattcc gctaatgggc
      121 aatattttta aagaagaaaa ggaaactata aaatattaac agcctcctag cgatgccgaa
      181 aagccctttg ataaaaaaag aatcatcatc ttaagaaatt cttagtcatt tattatgtaa
      241 atgcttataa attcggccct ataatctgat aaattattaa gggcaaactt atgtgaaagg
      301 gtgataacta tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg
      361 gttgaattat acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa
      421 gcattggaat gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc
      481 ggcacaagcg gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc
      541 atgctgaccg ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat
      601 gattatataa cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg
      661 cgccgataca aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc
      721 ggccttgtca ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt
      781 actcccaccg agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc
      841 tccgagctgc tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc
      901 atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa
      961 tatataaaaa cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc
     1021 caaactagaa cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt
     1081 cgtgttgtat attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt
     1141 ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg
     1201 gaacaatata gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg
     1261 cgtcatgctt tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact
     1321 tattcagaac gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa
     1381 gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca
     1441 aagaaaaaat gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat
     1501 tatcggttat ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa
     1561 gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt
     1621 tgagattaca cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata
     1681 ctatatgctg gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca
     1741 ggcggttatt cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag
     1801 agtctttaac aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga
     1861 cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat
     1921 cccaaaagat aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc
     1981 ttccgatacg ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca
     2041 tggagggcag atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc
     2101 agcgatgcca gacttggttg ataaaaggag gtcctaagag atgtatataa ttttggctct
     2161 ttgtcaaatg atgttggtaa agagaaatct ta
//
LOCUS       DACJGB010000246         1781 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.188, whole genome shotgun sequence.
ACCESSION   DACJGB010000246 DACJGB010000000
VERSION     DACJGB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.188"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            24..416
                     /locus_tag="IYI34_003249"
     CDS             24..416
                     /locus_tag="IYI34_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAR0561026.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IYI34_003250"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IYI34_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAR0561027.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DACJGB010000247          772 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.232, whole genome shotgun sequence.
ACCESSION   DACJGB010000247 DACJGB010000000
VERSION     DACJGB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.232"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="IYI34_003251"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="IYI34_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAR0561028.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          287 a          138 c          120 g          227 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      241 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
LOCUS       DACJGB010000248          772 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate V609
            SAMEA5368263-rid9531353.guided.233, whole genome shotgun sequence.
ACCESSION   DACJGB010000248 DACJGB010000000
VERSION     DACJGB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA5368263
            Sequence Read Archive: ERR3171090
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000699573.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000699573.1
            Genome Coverage       :: 29x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/08/2020 06:24:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,252
            CDSs (total)                      :: 3,194
            Genes (coding)                    :: 3,122
            CDSs (with protein)               :: 3,122
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 32 of 72
            Pseudo Genes (incomplete)         :: 38 of 72
            Pseudo Genes (internal stop)      :: 10 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA5368263-rid9531353.guided.233"
                     /isolate="V609"
                     /isolation_source="Hospitalized patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2013"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="IYI34_003252"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="IYI34_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAR0561029.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          286 a          137 c          121 g          228 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      241 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
